LOCUS       DATLGG010000001         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_94990, whole genome shotgun sequence.
ACCESSION   DATLGG010000001 DATLGG010000000
VERSION     DATLGG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94990"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..2811
                     /locus_tag="VM638_00005"
     CDS             259..2811
                     /locus_tag="VM638_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVM10843.1"
                     /translation="MKRTLALPVVLLAVLLAPTPASPSNDGDVTVRDSSYDPPTVTIS
                     PGQKVRWTSQGGLPHTITADDGSFDSHPACGSGGACMTAGQTYERTFPTAGTFRYYCK
                     LHGSPGGSGMAGTVVVTAPAEDLTDTSSTVATLSGSATTGAVSGTATFGGQKPVVVAD
                     DPANDANITPRVTDRDGTEAGVDLLNATMSVSNPNQPELLVRWQVADLPTERIVPEGV
                     RYTLPFKVGNAVYVVQAKFSNVTDPDHATNAAGHAGRLGASFELRGACNPSVQGTGIA
                     HCSHRGWLQGSVDPVSSTISVRVPVGSSMAPEIVPGAQLRRNDSSNPDLNDIIAGYQL
                     ASLTPSTPLDDRATFGPANAEGFSFTIPAKRVSLGTAPKGSDPRDVEYTTEATLQLNG
                     SFSGTVPVQGVAPGTFDIFARACFAQNCGYRSLGGATLATGNVTAALSLPEEGQADRS
                     YAFWRGGEVGDRIHYALPSQPQPFANCTSSPCFTFTVRVDTPGAARLRVALDASKRND
                     GFLLQVTSPGGASQSATNSNRWNSELFFSNPGTGVWTILVRPVSAEHAEFGMRAKLER
                     EIPARVADENGLLPPNLRPTAPFELGFEGPALFPANGPFIAPDDANPPAGVAGHGAYS
                     CTPDETLDGEAIRCLRFSFGLSNVGPGPFVIRGDGGNSTDVRPLFQCVERADGSALSR
                     PAGSQQFHPQHLHNHYLDLIELDLQRADPQAGTMTPVGDGRKLGYSPIEQAIGDWYAF
                     DQIPGTNNLACAGGDRRFALSIGWGDGYRYQRIGNFVDFGTNLDGHYVVRLKIDPLNH
                     VLESDERDNIAYTYIRVSQLDRIEILEYGRGEGPFDPNKQVLQLRRKGGVPW"
BASE COUNT          453 a         1096 c          980 g          392 t
ORIGIN      
        1 gttctggacg atgttcgtga tcggggagac cgtcgtgacg acccgcagcc ccggttccgg
       61 atcgaccgtg ccgtcggcgc agcctggaag cagagcagcc gcgaccacca ggacccccgc
      121 acgccgagcc acggcgcgag cataggacct tgttgcaatc gcttgcaaca agaccccccc
      181 tttgtgcacc tgcccaaagg gaaagtccga ggtcgcggcg aaggaaacga gcaggaccgt
      241 cgccgacctg gaggagtcgt gaagaggacg ctcgccctgc ccgtcgtgct gctcgcggtc
      301 ctgctcgccc cgacccctgc ctctccctcg aacgacggcg acgtgaccgt gcgggatagc
      361 tcctacgacc cgccgacggt gacgatctcc ccgggccaga aggtccgctg gacgagccag
      421 ggcggtctcc cccacacgat caccgcggat gacggctcct tcgactccca cccggcgtgc
      481 ggatccggcg gcgcgtgcat gaccgccggc cagacgtacg agcgcacctt ccccacggcg
      541 ggaacgttcc gctactactg caagctgcac ggctccccgg gcggcagcgg gatggcgggg
      601 accgtcgtcg tcacggcgcc agccgaggac ctgaccgaca cctcctcgac ggtcgcgacg
      661 ctctccggct cggcgacgac cggggccgtc tccgggaccg ccaccttcgg cggccagaag
      721 cccgtcgtgg tggccgacga cccggccaac gacgcgaaca tcacgccgcg ggtgaccgac
      781 cgcgacggga ccgaggcggg ggtcgacctg ctgaacgcca cgatgtccgt ctccaacccg
      841 aaccagcccg agctgctcgt gcggtggcag gtggccgacc tcccgaccga gcgcatcgtg
      901 cccgagggcg tgcgctacac gctgccgttc aaggtcggga acgccgtcta cgtggtgcag
      961 gcgaagttct cgaacgtcac cgaccccgac cacgcgacga acgcggccgg gcatgcaggg
     1021 cgcctcggcg cgagcttcga gctccgtggc gcctgcaacc ccagcgtcca ggggacgggg
     1081 atcgcgcact gctcgcaccg cgggtggctc cagggctcgg tcgatccggt gagctccacg
     1141 atctcggtgc gggtgccggt gggctcctcg atggcgccgg agatcgtccc gggagcgcag
     1201 ctgcgccgca acgactcgtc gaaccccgac ctcaacgaca tcatcgccgg ctaccagctg
     1261 gcctcgctca ccccgtcgac gccgctcgac gatcgcgcga ccttcgggcc cgcgaacgcg
     1321 gaggggttct cgttcacgat cccggcgaag cgcgtctcgc tcgggacggc gccgaagggg
     1381 tccgaccccc gcgacgtcga gtacacgacc gaggcgacgc tccagctgaa cggctcgttc
     1441 tccggcacgg tccccgtgca gggggtcgcg cccggcacct tcgacatctt cgctcgcgcg
     1501 tgcttcgccc agaactgtgg gtaccgcagt ctcggcggcg ccacgctcgc gaccgggaac
     1561 gtgaccgcgg cgctctcgct gccagaggag ggacaggccg accgcagcta cgccttctgg
     1621 cgcggcggcg aggtcggcga ccggatccac tacgcccttc cgagccagcc gcagccgttc
     1681 gcgaactgca ccagctcgcc ctgcttcacg ttcaccgtcc gggtcgatac gcccggcgcg
     1741 gcgcggctgc gggtcgcgct cgacgcgagc aagcgcaacg acggcttcct cctccaggtg
     1801 acatccccgg gcggcgcctc gcagtcggcg acgaactcga accggtggaa cagcgagctc
     1861 ttcttcagca accccggcac gggggtctgg acgatcctcg tccggccggt cagcgctgag
     1921 cacgcggagt tcgggatgcg cgcgaagctc gagcgggaga tccccgcgcg cgtagccgac
     1981 gagaacgggc tcctgccgcc gaacctgcgg ccgacggcgc cgttcgagct ggggttcgag
     2041 ggaccggcgc tcttcccggc gaacgggccg ttcatcgcgc cggatgacgc caacccgccg
     2101 gcgggcgtcg ccggccacgg cgcctactcg tgcacccccg acgaaaccct ggacggcgag
     2161 gcgatccggt gcctgcggtt ctccttcggc ctgtcgaacg tcgggcccgg accgttcgtg
     2221 atccgcggcg acggcggcaa ctccaccgac gtccgtccgc tcttccagtg cgtcgagcgc
     2281 gccgacgggt cggcgctctc gaggccggcg gggtcccagc agttccaccc ccagcacctc
     2341 cacaaccact acctggacct gatcgagctc gacctccagc gcgcggatcc ccaggccgga
     2401 acgatgacgc cggtcggcga cgggcggaag ctcggctaca gcccgatcga gcaggcgatc
     2461 ggcgactggt acgccttcga ccagatcccc gggacgaaca acctcgcctg cgcgggcggc
     2521 gaccgccggt tcgcgctgag catcggttgg ggcgacggct accgctacca gcgcatcggc
     2581 aacttcgtgg atttcggcac gaacctcgac ggccactacg tcgtgcgcct gaagatcgac
     2641 ccgctgaacc acgtcctcga gtccgacgag cgcgacaaca tcgcctacac ctacatccgc
     2701 gtctcgcagc tggaccggat cgagatcctc gagtacgggc gcggcgaggg gcccttcgac
     2761 ccgaacaagc aggtcctcca gctccggcgg aagggaggcg tcccgtggtg aggtctcgga
     2821 tcctcctcct cgcgctcgtc acagcggcct cggttccggc gggggccgac gccgccggtc
     2881 gcacgaacgc cacgcgccac gaggtggtga ccgccgggaa c
//
LOCUS       DATLGG010000002         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_95027, whole genome shotgun sequence.
ACCESSION   DATLGG010000002 DATLGG010000000
VERSION     DATLGG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95027"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VM638_00010"
     CDS             complement(<1..558)
                     /locus_tag="VM638_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10844.1"
                     /translation="MKRGMVVLAMAAMTAVPVAAPVSAGAGDPAGWYRVSFEDRLTQE
                     DRDALSRAGASDLQYLPVHAYRAWLPSPGAVRRLPGVASVRPLSAAERIDPSLPTSGP
                     APVLIGLRALAEGVSVAALARLGDVLSVEDADPAGEARHVVMRVPAGLIPSIAARSEV
                     AHVGPASPGLVPEDEASDQITAGNLS"
     gene            complement(608..2158)
                     /locus_tag="VM638_00015"
     CDS             complement(608..2158)
                     /locus_tag="VM638_00015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HVM10845.1"
                     /translation="MKRLALMLATLLLPPVAATAQEPDEPPVRTGADDATVVAVIDSG
                     ISPYHWDYLASRMPQATDEDPTNDLPLDAAPHTWLPGFPDPSAFASYNRFDLTLDEVN
                     AGRSITALDSQDSAKWNAVRQSTATAGHYYWFPQTKIIGGMTFASGGRIRAAASTHGM
                     GTASVSVGNIFGTCAECLLVFIQYGSVADGERAIEWALRQPWIDLISNSYGFSLVERD
                     RLYSGSDTALQRQASLRGQTVLFSAGNGISNTFTIPNSTLFSSQEGPDWIVTVGGVNP
                     NTGGSYTGHGKPADISGLGSAYPSSYGAGTVGNAGSNFSGTSNATPQVAGMYAKALFA
                     ARRSLPGPSRAQADGVIAVQDPDERLEERPFACAAARPSCELADGILTAKELRTRFFH
                     GATHTPAGMEVGQTSGLVGTPAIGEDEFLNEGHGTYYGKKNGAAAFAQEMERIVAPMQ
                     GRAQTLQRPAGEREWMIVDSYCRQEIWGDWTDGYFVRGQTELPGATPPGPLRLALQAS
                     CPALFPPV"
     gene            complement(2223..>2405)
                     /locus_tag="VM638_00020"
     CDS             complement(2223..>2405)
                     /locus_tag="VM638_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10846.1"
                     /translation="LAGKTLTVKAGSTTVTATTAGDGTFSANLGLTEGSREVTVTWSD
                     PTGIYRTARVTQTVSA"
BASE COUNT          319 a          854 c          865 g          367 t
ORIGIN      
        1 gctcaggttg ccggccgtga tctggtcgct cgcctcgtcc tcgggcacga ggccggggga
       61 tgccgggccg acgtgcgcga cctctgagcg ggcggcgatc gatgggatga gcccggcggg
      121 gacccgcatc acgacatggc gggcttcgcc cgcgggatcg gcgtcctcga cgctcagcac
      181 gtcccccaag cgtgccagcg cggccacgga gaccccttca gcaagtgcgc ggagcccgat
      241 gagcaccgga gcgggcccgg acgtcggaag ggacgggtcg atcctctccg cggcgctcag
      301 ggggcgtacg gacgctacgc ccgggagacg tcggaccgcg cccgggctcg gcagccaggc
      361 gcggtaggcg tggaccggaa ggtactggag gtcactcgcg cccgctctag agagagcgtc
      421 gcggtcctcc tgggtcaggc ggtcctcgaa cgagacgcgg taccagcctg caggatcccc
      481 ggcgccggcg ctcaccggcg ccgccaccgg gaccgcggtc atcgcggcca tcgccagcac
      541 gaccatcccc cgcttcatcc gcacctcctg tcagcgtgac ggtcccgcga ccgcccctcc
      601 ccctcgttca gaccggcggg aacagcgccg ggcacgacgc ctggagggcc aggcgcaacg
      661 gtccgggcgg cgtcgcaccc gggagctcgg tctgcccacg cacgaagtag ccgtcggtcc
      721 agtcgcccca gatctcctgg cggcagtagg agtcgacgat catccactcc cgctcgccgg
      781 ccgggcgttg gagcgtctgg gcgcgcccct gcatcggcgc gacgatccgc tccatctcct
      841 gtgcgaacgc cgcagccccg ttcttcttgc cgtagtacgt cccgtggccc tcgttcagga
      901 actcgtcctc gccgatcgcc ggcgtcccga cgaggccgct cgtctggccc acctccatgc
      961 cggccggcgt gtgcgtggcc ccgtggaaga agcgagtgcg aagctccttg gccgtcagga
     1021 tgccgtcagc aagctcgcag gacgggcggg cggccgcgca ggcgaacggc ctctcctcca
     1081 gccgctcgtc gggatcctga accgcgatca ccccatccgc ctgggcgcgg ctcgggccgg
     1141 gcaggctgcg ccgcgcggcg aacagcgcct tggcgtacat ccccgcgacc tgcggcgtgg
     1201 cgttcgaggt cccgctgaag ttcgaccccg cgttcccgac cgtcccggcg ccgtaggagc
     1261 tcggataggc gctgccgagc ccggagatgt ccgccggttt gccgtgaccc gtgtaggagc
     1321 cccccgtgtt cgggttgacc ccgccgaccg tcacgatcca gtccggtccc tcctgcgagc
     1381 tgaacagcgt gctgttcggg atcgtgaacg tgttggagat gccgttcccg gcggagaaga
     1441 gcaccgtctg cccgcgcagg ctcgcctgcc gctggagggc ggtgtcggag ccgctgtaga
     1501 ggcggtcgcg ctcgacgagg ctgaacccgt aggagttcga gatcaggtcg atccacggct
     1561 gccggagcgc ccattcgatg gcccgctcgc cgtccgcgac cgacccgtac tggatgaaca
     1621 ccaggaggca ttcagcgcag gtcccgaaga tgttgccgac ggagacgctg gccgtcccca
     1681 tgccgtgcgt gctggcagcc gcgcggatcc ggccgccgct cgcgaacgtc atccccccga
     1741 tgatcttcgt ctgggggaac cagtagtagt gcccagccgt tgccgtgctc tgtcggacgg
     1801 cgttccactt cgccgagtcc tgcgagtcga gcgccgtgat gctgcgtccg gcgttcacct
     1861 cgtcgagcgt gaggtcgaag cggttgtagc tggcgaacgc cgatgggtcg gggaagcccg
     1921 gaagccaggt gtgcggcgcg gcgtcgagcg ggaggtcgtt cgtcgggtcc tcatcggtcg
     1981 cctgcggcat cctcgaggcg aggtagtccc agtggtaggg actgatcccg gagtcgatca
     2041 cggccacgac cgtggcgtcg tcggcgccgg tgcggaccgg cggctcgtcg ggctcctgcg
     2101 cggtcgcggc gaccggcggg agcaggagcg ttgcgagcat cagggcgagc cgcttcacgt
     2161 ttcgggcctc ctctcgaacg agcgatgagg ggcggcccgg aggccgcccc tcatcgcatc
     2221 acctatgcgg agacggtctg ggtgaccctc gcggtgcggt agatccccgt ggggtcgctc
     2281 caggtcaccg tcacctcccg gctgccctcg gtgagaccga ggttcgccga gaaggtgccg
     2341 tcgccggcgg tcgtggccgt gacggtcgtg ctgcccgcct tcacggtgag ggtcttgccg
     2401 gcgag
//
LOCUS       DATLGG010000003         9579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_31760, whole genome shotgun sequence.
ACCESSION   DATLGG010000003 DATLGG010000000
VERSION     DATLGG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31760"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VM638_00025"
     CDS             <1..1480
                     /locus_tag="VM638_00025"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVM10847.1"
                     /translation="GRASAARAKVLRMLDVVREAVEAARAAGADYADARFVSEDEESI
                     TVKNQEMEGIDRSHTEGVGIRVLAGGHWGFAATARTDAADLRRTAELAVGIARAAARL
                     PGEPVRLAEVEPVRDSWQTLIQEDPFSVPLDEKVALLMEASRRMQQVDGLAFAEASLD
                     FFRRSTTFVSSEGAEIDQLIVNSGGGLEATAIGDGELQRRSFPNSFRGHLAAAGYEHI
                     RTLGLAEEAERVGSEAVRLLSAPDCPSEEATLIVDGPQMVLQIHESIGHPIELDRVLG
                     MEEAYAGTSFLSPDDRGRLRYGSDLVSIYADATLPGGLGTFGYDDEGVPAQRVPIIEE
                     GIFRNFISSRETAAVLGESSNATMRADGWSNLPLIRMTNISIAPGDGTLAELIGDTKD
                     GIFMSTNSSWSIDDKRVNFQFGCEAAWRIRDGRLTEMYKNPNYTGITTEFWGSCDAVC
                     GPEDWTVWGTPNCGKGQPGQTARVGHGAAPARFRGVRVGVRR"
     gene            1477..2826
                     /locus_tag="VM638_00030"
     CDS             1477..2826
                     /locus_tag="VM638_00030"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVM10848.1"
                     /translation="MTGPIGPDEARSVAEAALDLKGAEVEVLFAHEWGGLTRFAESSI
                     HQSTWREDTGLRIRVVAGGRVGVTATNDFTKDGAAKAARSALELAETAAPDPRFPGLA
                     PPAEVPERPGAYDDATAGATPEERAEAVEALVGRCGAGFHAAGAYETTAMEAAILNSE
                     GHFCYAASTKATVTTVVSGGEGGAGFADAASTRRADIDPAEVGARAAAKAEGSQRPRD
                     LDPGRYEVVLEPAAVTTLAAFLAYMGFGGRMIAEGRSCFSGRLGEQLMAPSVTIYDDA
                     LSPETIGLPFDFEGTPKRRVDLVRDGVILGGVHDRRSAAEAGTASTGHALPPPNPEGP
                     FPLNLFMAPGEATVEEMVGGVERGLLVSRFHYSNIVHPTEAVITGMTRDGTWWIEGGE
                     IAYPVKNLRFTQSIIEALHDVVDVGRETHLASEFFFEASRVPPLRIGSFQFTGKSDH"
     gene            complement(2843..3055)
                     /locus_tag="VM638_00035"
     CDS             complement(2843..3055)
                     /locus_tag="VM638_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVM10849.1"
                     /translation="MSASRLDPLTPRQREVLALVADGRTTHEIARILRLSPHTVKNYL
                     ERIYERLGARDRTQAVAIALRGGFLD"
     gene            3117..4112
                     /locus_tag="VM638_00040"
     CDS             3117..4112
                     /locus_tag="VM638_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10850.1"
                     /translation="MLTTLGLLASGGIVLYLGAETAVRGASAFARRAGIPVFVLGALL
                     FGLDVESMGTAVTAASRGATQVAAGSIFGSVLFLFSAGFGAALLVSRRPIPSPGNAMV
                     IAPCLPLGAATIALTDRFVSGSEGLILIGFYAIYVALLFRLRRADEEAPVEARAGEDS
                     IDDLPDDGAETSEVHASEASPPPSPRLAPAGAALAGLALLAAGAWLLVEGGTRLIETT
                     TLFPGFVGAAVVGVLTSLDEVLLEIVPVRRGTPELATGNLFGTIAAFSSGLLGLAAVV
                     RPLDLDGAANAAFLGAVFLYAVVAVTFVLRGRAGRLLGGFVLAAYAAWLVATGSV"
     gene            4152..4610
                     /locus_tag="VM638_00045"
     CDS             4152..4610
                     /locus_tag="VM638_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVM10851.1"
                     /translation="MQVGDIMTKDVKTVSAEDTFAHASKVLHDNGISSVVVAGEAGPA
                     GIVTERDLVNLVAEGADPREVRVGDRMTKELATVEPRTDLTEASRLMADRRIRHLPVV
                     DRGTLVGIISIRDLTRWAVGELTGGHELPDIERSQAALSAAVEAKREPAS"
     gene            complement(4607..5266)
                     /locus_tag="VM638_00050"
     CDS             complement(4607..5266)
                     /locus_tag="VM638_00050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVM10852.1"
                     /translation="MPVEVVFFDVGGPLYGDRPYYEALLRAIREARPDADADAYWTEF
                     EAARRDQNGSFTRRLTRLFVAPGEVEAVIARGQQLWEYPPETLQPDAAACLEALHGRY
                     RLGVLANQEPWIRETLARDGLVGYFDVWAVSGELGVEKPDPAIFRHALDSADALPERC
                     VMVGDRLDNDVTPARAHGMRAIWLLRGEAPDDPTAAQLAEADAAIRGLDELPETLQRL
                     G"
     gene            5299..6498
                     /locus_tag="VM638_00055"
     CDS             5299..6498
                     /locus_tag="VM638_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVM10853.1"
                     /translation="MNRIPSLVAVGITLAACSPEPRIIEPAPTSPTPGLPSPPQELHL
                     DELELELEPFADGFEAPLFLTHAGDGSGRTYVVEQGGRIRVVEPDGTVRPRPFLDIVD
                     RVTSGGEQGLLGLAFHPEDRSRVFVNYTGRGGETVVAEYRAGSPTQADPGSERVLLRI
                     EQPYSNHNGGMLAFGPEGYLWIGTGDGGGGGDPEGNGQDPSTLLGAILRISVTGDPYG
                     IPGGNPFALGGGGREEVWAYGLRNPWRFSFDRRTGDLWIGDVGQHELEEINVHPSAMS
                     GAANFGWNIMEGSRCYEAPDCDTDGLVLPVTEYPTSLGCAVTGGYVYRGRRFPQMRGA
                     YLFADYCAGQIWGLDAAEEGVYDGAVEHRELLDTGLSISSFGEDELGELYVTDLAGGG
                     VHRVVAR"
     gene            complement(6500..7333)
                     /locus_tag="VM638_00060"
     CDS             complement(6500..7333)
                     /locus_tag="VM638_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVM10854.1"
                     /translation="MTIDLHTHTTFSDGTFTPAEMVRLALQRGLTAISISDHDSTEGV
                     SEAVAAAEGTGLEIVPGVEFSTVYDGGGIHVLTYWPDLGESEFQEELRRLRDDRWTRG
                     EGMVRKLRELGYPIEFERVREIARGNSIGRPHVAQALVEAGVVPTIKDAFSPDLIASG
                     GRAYVEKHALDPVEAVRLIKRAGGLAVLAHPVHWRDGLPVPDEVIERMVEAGLDGIEV
                     AHPDQDPSDRARYAEMAKRLGLVATGSSDCHGARYEPIRMGSVTTDPGEFARLKERKG
                     L"
     gene            7459..7725
                     /locus_tag="VM638_00065"
     CDS             7459..7725
                     /locus_tag="VM638_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10855.1"
                     /translation="MNEQLTTRDREAATEPADGLLLAPVKGRSAWDAVQALRAEMQRL
                     AASGEPHATFVTAIATDGREVTATDPAAEVLALGYEVRPAARAI"
     gene            7846..>9579
                     /locus_tag="VM638_00070"
     CDS             7846..>9579
                     /locus_tag="VM638_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVM10856.1"
                     /translation="MRSHRPVLAATLGLALLGSGVAAASPAPREPHLFTRPAYPTLVE
                     PRASAMADMDGDGREDLVHAVGLASSQPSIGISVMRGRAVGFEHPTLHPTGALTHALQ
                     VLDIDADGHRDVATLRRIDQGSATTAVLPGTGNGSLGDSIAMPFPLHRDATFGRFTAG
                     ALPGAVVAGESSLRAWRQLPGYTFLQDRVVLAGSAEHLLALDLEGDGVDELALVADGR
                     LRLFRNLSEAASAPTPPLRRLEALDVTGDGRPEIVALTSDGSVRAFDGALGTVRTMAG
                     TQQGVRVAAGDVDGDGAIDLVRLNEGISASVAFASGGRVEVPLARAGLAVYVHDATGD
                     GNPDLLVPSTASSVVEVVEGDGRGGFSRQREPVFAQPRGWAVGVADFNRDGKRDFVAG
                     ALNYLLDGYATFSTATIMLGDGAGRFTPRTTISTQGASTGLDVGDVNGDGNPDVLVSD
                     YHSGSVMQHLGRGDGTFEERIPVPGCALADTVVIDDLNGDGIDDAAAACRGTIWRAYV
                     SVWLGSPAGLVPTGQIHVSQNSAALWIRTGDVNGDGNRDIAIGSFDHFPVRPECAPDP
                     LCLIGTTGRGIG"
BASE COUNT         1338 a         3390 c         3502 g         1349 t
ORIGIN      
        1 tggccgagcg tcggcggccc gcgctaaggt gctgcgcatg ctggatgtgg tccgggaggc
       61 cgttgaagcc gcgcgtgcgg cgggggcgga ctatgccgat gcccgcttcg tctccgaaga
      121 cgaggagtcc atcaccgtca agaaccagga gatggaaggg atcgaccgct cccacaccga
      181 aggcgtcggg atccgcgtcc tggccggagg gcactggggc ttcgcggcca cggcgcgcac
      241 ggacgccgcc gaccttcggc gcacggcgga gctggccgtg gggatcgccc gagcggccgc
      301 gcgcctgccg ggcgaacccg tgaggctcgc cgaggtcgag ccggtccgcg acagctggca
      361 gaccctgatc caggaggacc ccttcagcgt cccgctcgat gagaaggtcg ccctcctgat
      421 ggaggcgtcc cggcggatgc agcaggtgga cgggctcgcc ttcgccgagg cctcgctcga
      481 tttcttccgg cgtagcacga ccttcgtctc ctcggagggc gccgagatcg atcagctgat
      541 cgtgaactcc ggtggcggcc tcgaggccac cgcgatcggc gacggcgagc tccaacgccg
      601 gtccttcccg aactccttcc gtgggcacct cgccgcggcc ggctacgagc acatccgcac
      661 gctcggactg gccgaggagg ccgagcgggt gggcagcgaa gcggtgcggc tgctctcggc
      721 cccggactgc ccgagcgaag aggcgaccct gatcgtcgac ggaccgcaga tggtcctgca
      781 gatccacgag tcgatcgggc acccgatcga gctcgatcgc gtcctcggga tggaggaggc
      841 ctacgcggga acctcgttcc tctcgccgga tgaccgcgga cggctccgct acggatcgga
      901 tctggtttcg atctacgccg acgcgacgct cccgggcggg ctcggaacct tcggctacga
      961 cgacgagggg gtgcccgccc agcgggttcc gatcatcgaa gagggcatct tccggaactt
     1021 catctcgagc cgcgagaccg cggcggtgct cggggagagc tcgaacgcga cgatgcgcgc
     1081 cgacgggtgg tcgaacctcc cgctgatccg catgacgaac atctcgatcg cccccgggga
     1141 cgggaccctc gccgagctga tcggcgacac gaaggacggc atcttcatgt cgacgaactc
     1201 ctcgtggtcg atcgacgaca agcgggtgaa cttccagttc ggctgcgagg ccgcctggcg
     1261 gatccgcgac ggccgactga ccgagatgta caagaacccc aactacaccg gcatcacgac
     1321 cgagttctgg ggctcctgcg acgccgtctg cggcccggag gactggacgg tgtggggaac
     1381 gcccaactgc ggcaagggcc agccgggcca gacggcgcgc gtcggccacg gcgccgcgcc
     1441 ggcacggttc cggggggtcc gtgtgggggt gcggcggtga ccggaccgat cgggccggac
     1501 gaggcgcgct ccgtcgccga agcggcgctc gacctgaagg gcgcggaggt cgaggtcctg
     1561 ttcgctcacg agtggggcgg cctgacgcgg ttcgccgaat cgtcgatcca ccagagcacc
     1621 tggcgcgagg acacagggct gcggatccgg gtcgtcgccg ggggacgcgt cggcgtgacg
     1681 gcgacgaacg acttcacgaa ggacggcgcg gcgaaggcgg cgcgcagcgc cctcgagctg
     1741 gcggagaccg cggcgccgga cccgcgcttc ccgggcctcg ccccaccggc ggaggttccc
     1801 gagcgaccgg gggcctacga cgacgcgacc gccggcgcga cacccgagga gcgagccgag
     1861 gcggtcgagg ccctcgtcgg acgctgcggg gccgggttcc atgcggccgg cgcgtacgag
     1921 accaccgcga tggaggccgc gatcctcaac agcgagggcc acttctgcta cgcggcctcg
     1981 acgaaggcga ccgtcacgac ggtcgtctcc gggggcgagg gtggggcggg cttcgccgat
     2041 gcggcgtcaa cgaggagggc cgacatcgac ccggcggagg tgggcgctcg tgccgcggcg
     2101 aaggcggagg gttcacagcg gccgcgcgac ctcgaccccg gccggtacga ggtcgtgctg
     2161 gagccggcgg ccgtgacgac cctggccgcg ttcctcgcct acatggggtt cgggggcagg
     2221 atgatcgccg agggccggtc ctgcttctcc ggccgcctcg gcgagcagct catggcgccg
     2281 agcgtgacga tctacgacga cgcgctctcg ccggagacga tcgggctgcc cttcgacttc
     2341 gagggaacgc cgaagcggcg ggtcgacctc gtccgcgacg gggtgatcct cggcggcgtc
     2401 cacgatcgcc ggagcgcagc cgaggcgggc acggcgtcga cgggtcacgc cctcccgccg
     2461 ccgaacccgg agggcccgtt ccccctgaac ctcttcatgg cgccgggcga ggccaccgtc
     2521 gaggagatgg tcggtggggt ggagcgcggc ctcctcgtct cacgcttcca ctactccaac
     2581 atcgtccacc cgacggaggc ggtcatcacc gggatgaccc gcgacgggac atggtggatc
     2641 gaggggggcg agatcgcata cccggtgaag aacctgcgct tcacccagtc gatcatcgag
     2701 gcgctccacg acgtggtcga cgtcgggcgc gagacgcacc tcgcgagcga attcttcttc
     2761 gaggcgtcgc gcgtcccgcc gctgcggatc ggcagcttcc agttcaccgg gaagagcgac
     2821 cactaggccg ctccaggggt cgctagtcca ggaagccgcc gcggagggcg atggcgaccg
     2881 cctgcgtccg gtcccgcgca ccgagccgtt cgtagatccg ctcgaggtag ttcttcaccg
     2941 tgtggggcga gagccgcagg atccgggcga tctcgtgggt cgtccgcccg tccgcgacga
     3001 gcgcgaggac ctcccgctga cgcggcgtga gggggtcgag ccgtgaggcg ctcatggatc
     3061 cagtttcccc cggatcgagg gcgattccca gcaagccatc cgctaggctc ccgcccgtgc
     3121 tcaccaccct cggtctcctc gcgagcggcg ggatcgtgct ctacctcggg gctgaaaccg
     3181 ccgtccgcgg cgcctcggcc ttcgcccgcc gggcggggat ccccgtcttc gtcctcggcg
     3241 cgctcctgtt cgggctcgat gtggagagca tggggacggc ggtcacggcc gcgtcccgag
     3301 gtgcgacgca ggtcgccgcg ggttccatct tcgggtcggt cctgttcctg ttcagcgccg
     3361 ggttcggcgc cgcgctcctc gtctcgcggc gacccatccc ttctcccggg aacgccatgg
     3421 tcatcgcacc ctgcctgccg ctgggggccg cgacgatcgc gctcaccgat cgattcgtct
     3481 ccggctccga aggactgatc ctgatcggtt tctacgcgat ctacgtcgcg ctcctgttcc
     3541 gcctgcgtcg tgcggacgag gaggcccccg tcgaggcgcg ggcgggcgag gacagcatcg
     3601 acgacctccc ggacgacggg gcggagacct ccgaggtgca cgcgagcgag gcgtcgcccc
     3661 cgccgtcgcc gcggctggcg ccggcagggg cggcgctggc ggggctcgcg ctcctcgccg
     3721 ccggcgcctg gctcctggtc gaagggggga cgcggctgat cgagaccacc accctgttcc
     3781 ccgggttcgt cggcgcggcg gtcgtcgggg tcctcacgag cctcgacgag gtcctgctcg
     3841 agatcgtgcc cgtccgacga gggacgccag agctggcgac ggggaacctc ttcggcacga
     3901 tcgcggcgtt ctcctcgggg ctcctcgggc tggcggccgt cgtccggccg ctcgacctcg
     3961 acggcgcggc gaacgcggcg ttcctcgggg cggtcttcct ctacgccgtc gtggcggtga
     4021 cgttcgtgct tcggggccgg gccgggcggc tgctcggggg gttcgtcctc gcggcctacg
     4081 ccgcctggct ggtcgcgacg ggctcggtgt aggctggcgg tccgcccact ccaccgtgca
     4141 aggaggttcg catgcaggtc ggcgacatca tgacgaaaga cgtcaagacg gtgtcagcgg
     4201 aggacacctt cgctcacgcg tcgaaggtcc tgcacgacaa cggcatctcc tcggtcgtcg
     4261 tcgccggcga ggcgggcccg gcggggatcg tcacggagcg cgacctcgtg aacctcgtcg
     4321 ccgagggagc ggacccccgc gaggtgcggg tcggagaccg gatgacgaaa gagctcgcga
     4381 cggtggagcc gcggacggac ctgaccgagg catcgcgtct catggccgac cgccggatcc
     4441 ggcacctgcc cgtcgtcgac cgcgggaccc tcgtgggcat catctcgatc cgcgacctca
     4501 cgaggtgggc cgtcggcgag ctcacgggcg ggcacgagct cccggacatc gagcgcagcc
     4561 aggcggcgct gagcgccgcg gtcgaggcca agcgggagcc ggcgtcctag ccgagccgct
     4621 ggagcgtctc gggcagctcg tcgagaccgc ggatcgccgc atccgcctca gccagctgcg
     4681 ccgccgtggg gtcgtcgggt gcctcgcccc gcaggagcca gatggcccgc atcccatgcg
     4741 ctcgtgccgg ggtcacgtcg ttgtcgagac ggtcgccgac catgacgcag cgctcgggga
     4801 gcgcgtcggc cgaatcgagc gcgtggcgga agatcgccgg gtcgggcttc tcgaccccga
     4861 gctcgcccga gaccgcccag acgtcgaagt acccgaccag gccgtcgcgc gcgagggtct
     4921 ctcggatcca gggctcctgg ttggccagga cgccgaggcg gtagcggccg tggagggcct
     4981 ccaggcaggc ggccgcgtcc ggctggagcg tctccggcgg gtactcccac agctgctgtc
     5041 cccgcgcgat caccgcctcg acctccccgg gcgcgacgaa cagacgggtg aggcgtcgcg
     5101 tgaagctgcc gttctggtcg cggcgggcgg cctcgaactc cgtccaatac gcatcggcat
     5161 ccgcgtcggg gcgagcctcg cggatcgccc gcaggagcgc ctcgtagtag ggccggtctc
     5221 cgtacagcgg cccaccgacg tcgaagaaga ccacctcgac cggcacgcgc gcagtctagc
     5281 cccggcaggg gatgggccgt gaaccgcatc ccgagcctcg tcgccgtcgg catcacgctg
     5341 gccgcgtgct cgccggagcc caggatcatc gaaccggctc cgacatcccc gacccccggc
     5401 ctcccctcgc cgccgcagga gctccacctg gacgagctcg agctcgagct cgagccgttc
     5461 gcggatgggt tcgaggcgcc gctgttcctg acccacgctg gcgacggatc cggccgcacg
     5521 tacgtggtgg agcagggcgg gcggatccgt gtggtggagc cggacggcac cgtccggccc
     5581 cggcccttcc tcgacatcgt cgaccgcgtg acctccgggg gcgaacaggg cctgctgggc
     5641 ctcgccttcc atccggagga ccgctctcgc gtgttcgtca actacaccgg ccgcggcggc
     5701 gagaccgtgg tcgccgagta ccgagccggg tcccccacgc aggcggaccc gggctcggag
     5761 cgcgtgctgc tccggatcga gcagccgtac tcgaaccaca acggcgggat gctggctttc
     5821 ggcccggagg gctatctgtg gatcgggacc ggcgacggcg gcggaggcgg ggaccccgag
     5881 ggcaacgggc aggacccgag cacgctcctg ggggctatcc tgcggatctc ggtgaccggc
     5941 gacccctacg gcatccccgg gggcaatccc ttcgctctag gcggtggcgg tcgggaggag
     6001 gtctgggctt acggtcttcg caacccgtgg cgcttcagct tcgaccgacg gacgggcgac
     6061 ctgtggatcg gtgacgtcgg ccagcacgag ctggaggaga tcaacgtgca tccatcggcc
     6121 atgtcgggag ccgccaactt cggctggaac atcatggagg gcagccgctg ctacgaggcc
     6181 ccggattgcg acaccgatgg gctggtcctg cccgtgaccg agtaccccac ctcgctcggg
     6241 tgtgccgtca ccggcggcta cgtctaccgg gggaggcgct tcccccagat gcgcggggcg
     6301 tacctgttcg ccgactactg cgccgggcag atctgggggc tggatgccgc tgaggaaggg
     6361 gtctacgacg gggcggtcga gcaccgggag ctgctcgata ccggcctgtc gatctcctcg
     6421 ttcggggagg acgagctggg ggagctgtac gtgacggacc tcgccggcgg cggcgtccac
     6481 cgcgtggtcg cccgttgagc tagaggccct tccgctcctt cagacgggcg aactcgccgg
     6541 gatcggtcgt gacgctgccc atccggatcg gctcgtaccg cgcgccgtgg cagtcggagg
     6601 accccgtcgc gacgagcccg aggcgcttcg ccatctcggc gtagcgcgcg cggtcggagg
     6661 gatcctggtc ggggtgggcg acctcgatgc cgtcgagccc cgcttcgacc atccgctcga
     6721 tgacctcgtc ggggaccggc aggccgtcgc gccagtggac gggatgggcc agcaccgcga
     6781 ggccgcctgc ccgcttgatc agccggaccg cctcgacggg gtccagggcg tgcttctcga
     6841 cgtacgcgcg tccgcctgag gcgatcaggt ccggggagaa ggcgtccttg atcgtcggga
     6901 cgacccccgc ctcgaccagg gcctgcgcca cgtgcgggcg cccgatgctg ttgccccggg
     6961 cgatctcgcg gacccgctcg aactcgatcg ggtagccgag ctcccgcagc ttgcggacca
     7021 tcccctcacc gcgcgtccag cggtcgtcgc ggagccgccg gagctcctcc tggaactcgc
     7081 tctcgccgag atcgggccag tacgtgagga cgtggatccc cccgccgtcg tagacggtcg
     7141 agaactcgac gcccgggacg atctcgaggc cggtcccctc cgcggccgcc acggcctcgg
     7201 acaccccctc ggtcgagtcg tggtcgctga tggagatcgc ggtcagcccg cgttggagcg
     7261 ccaggcggac catctcggct ggggtgaagg tcccgtcgga gaaggtcgtg tgggtgtgca
     7321 gatcgatcgt caagggacgc gcatcctccc acacggcgcc gacacacatc cagaaaaaga
     7381 tcctcgatca cccgctgggt cggggctcct ccttcgttct atcgggtgaa gggcaccaca
     7441 cgagcgctgg aagggaccat gaacgagcag ctgacgacca gggaccgaga ggccgcgacc
     7501 gagccggccg atgggctgct cctcgcgccc gtgaagggcc ggagcgcctg ggacgccgtc
     7561 caggccctcc gggcggagat gcagcgcctg gccgccagcg gcgagccgca cgcgaccttc
     7621 gtcacggcga tcgcgaccga tggccgtgag gtcaccgcca cggaccccgc cgccgaggtg
     7681 ctcgccctcg ggtacgaggt ccgccccgcc gcccgcgcca tctgagcgaa cccacgccgg
     7741 gcccggcgcc tgaaatctgt gcatctgccc aaaggaccct gccccctcca ggtcgtatcc
     7801 ctccgcgccc ggggcgctcg cgccccctga ccgaaggagg gaacgatgcg gagccaccga
     7861 cctgtcctgg ccgccacgct gggcttggcg ctgctcggga gcggcgtcgc cgccgccagc
     7921 cccgcgccac gggagcccca cttgttcacc cgtcccgcct acccaacgct cgtcgagccg
     7981 cgcgcgagcg cgatggcgga catggacggg gatgggcggg aggatctcgt gcacgccgtc
     8041 ggtctggcct cgagccaacc ctcgatcggg atctcggtga tgcggggtcg cgcggtcggc
     8101 ttcgagcacc ccaccctcca cccgacgggc gcgctgacgc acgccctgca ggtgctggac
     8161 atcgacgccg acggacaccg tgacgtcgcg accctccgcc ggatcgacca agggtcggcg
     8221 accaccgccg tcctgccggg cacggggaac gggagcctcg gcgacagcat cgcgatgccg
     8281 ttcccgctgc accgggacgc gacgttcgga aggttcacgg cgggagccct gcccggcgcg
     8341 gtcgttgcgg gcgagagctc cctccgcgcg tggcgccagc tccccggtta cacgttcctc
     8401 caggaccggg tcgtgctcgc ggggagcgcg gagcacctgc tggccctcga cctggagggc
     8461 gacggggtcg acgagctggc cctggtcgcc gacgggcgtc tgcggctctt ccgcaacctc
     8521 agcgaagccg ccagcgcccc cacacccccg ctccgccgcc tcgaggctct cgacgtcacc
     8581 ggtgacgggc ggccggagat cgtcgcgctg acctcggacg ggtccgtgcg agcgttcgac
     8641 ggcgcgctcg ggaccgttcg gacgatggcg ggaacccagc agggcgtccg cgtcgcggcg
     8701 ggcgacgtgg acggggatgg agcgatcgac ctcgtgcgcc tgaacgaggg gatctccgcg
     8761 agcgtcgcct tcgcctcggg aggacgggtc gaggtcccgc tggcccgtgc ggggctcgcc
     8821 gtctacgtgc acgacgccac cggcgacggc aatccggacc tcctggtccc atcgacggcg
     8881 agctcggtgg tcgaggtcgt ggagggggac ggtcgcggcg gcttcagccg tcagcgcgag
     8941 ccggtcttcg cccagccgcg ggggtgggcg gtcggcgtgg cggacttcaa ccgcgacggg
     9001 aaacgggact tcgtcgcggg cgcactcaac tacctgctgg acggctacgc aaccttcagc
     9061 accgccacga tcatgctggg cgacggggcc gggcgcttca cgcctcgcac gacgatctcg
     9121 acgcagggcg cgtcgaccgg cctcgacgtc ggtgatgtga acggtgacgg caacccggac
     9181 gtcctcgtga gcgactacca ctcgggcagc gtgatgcagc acctcggccg tggcgatggc
     9241 accttcgagg agcggatccc agtgcccgga tgcgcgctcg ccgatacggt cgtcatcgac
     9301 gacctcaatg gcgacggcat cgacgatgcg gcggcagcgt gccgcgggac gatctggcgg
     9361 gcttacgtca gcgtgtggct cggatcgccg gcggggctgg tgcccacggg tcagatccat
     9421 gtctcgcaga acagtgcggc gctgtggatc cggaccggcg acgtcaacgg ggatggcaac
     9481 cgcgacatcg cgatcggctc gttcgaccac ttcccggttc ggccggagtg cgcgccggac
     9541 cccctctgcc tgatcgggac aaccggtcgg gggatcggc
//
LOCUS       DATLGG010000004         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_15982, whole genome shotgun sequence.
ACCESSION   DATLGG010000004 DATLGG010000000
VERSION     DATLGG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15982"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VM638_00075"
     CDS             complement(<1..185)
                     /locus_tag="VM638_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10857.1"
                     /translation="MSLSDAPANRPERVDVLVRGCDAVTMDDAGTVVSDAAIAVAGGR
                     IVWIGRSRDAGALPKAET"
     gene            complement(201..1220)
                     /locus_tag="VM638_00080"
     CDS             complement(201..1220)
                     /locus_tag="VM638_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM10858.1"
                     /translation="MRQARRDFLKLAAASGVAAPLFGRELLAQTRPKVRVQIGWIPNV
                     QYAGEWIALDRGLLTAQGIDVEWYPGGPNALPAPVMLAAGRADLGYSTWFPFIDAVAQ
                     GNDLVMIAAVFPKNPLGIISLAKKPIREPKDLVGARILAQGPNEKTAIEATLSLAGLP
                     VQWTQVPAGFSPEPLLAGDGDGYTAFGTNQTITLERMGLVRGKDFHFVSFDELGFRTY
                     GAVLMTTRRYLDANRPQVVAYVRGIIKGWEQNEADPKVAAELAVKKYGADFGLDLPQQ
                     IRQNELQIPLTRYEGAPRPRLALDREVIEGPMFRAARATGRTKLPDVDRAVDFSLVPE
                     AHRGL"
     gene            1359..2225
                     /locus_tag="VM638_00085"
     CDS             1359..2225
                     /locus_tag="VM638_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HVM10859.1"
                     /translation="MPERIRQGYPGLTGALRAFADFVLREPVRAARMSIHAAVEAAGV
                     SVATGNRFARAIGFENYPAFRADLIESLASAYEPVKRLEQQISKRSSSHDIMVGSLRE
                     DVSNLEETVGGLDEATCRRAVEMILGARRIFVLGFDNGACLAQILANGLMLLKDNVAH
                     VANGGGGAGVARHLSGFDGSDLVIAIAFPRYMKDTISLAGLARSREVPVLAITDSHHS
                     PLAALATVSLHACSRRQFASVSNAAALAVVEALLAAVAHRTPKSVHRAEAFTACALPW
                     IEAGPARRPRSD"
     gene            complement(2263..3036)
                     /locus_tag="VM638_00090"
     CDS             complement(2263..3036)
                     /locus_tag="VM638_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HVM10860.1"
                     /translation="MSEVEWWRLRAAEIRERSRPETVVIVPVASTEQHGPHLTTEVDC
                     ALVGEIARRAARIAEAQCPVLVTPVLWTGLAEHHMSLGGTITLDFATFSALVKGVCRS
                     LVRQGFGRILLLNGHGGNIAALTVIVNEIAPELDSPIATATYWLLAAKEFGAILEDQP
                     NVRHACEAETSMMLAIRPERVDMLAAAETVGPTGREVADVIGSEAAHRWRSFEARTSH
                     GVIGVPRRASAAKGEALLQAAAEAVSRCVIAREFWDLEA"
     gene            complement(3033..3818)
                     /locus_tag="VM638_00095"
     CDS             complement(3033..3818)
                     /locus_tag="VM638_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVM10861.1"
                     /translation="MTDSLVRLQGVVKRFGPETVLDRVTLSVEPAQVLVVIGPSGSGK
                     STLLRCIAGLEAIQRGRIWIADSLVAVGEEEPDGRSAPRRTKRHDVGMVFQQFNLFPH
                     LTVLRNITLAPQRVRGVSREQAEAIAVDLLRRVGLEDKKDAYPGRLSGGQQQRAAIAR
                     ALAMEPRIMLFDEVTSALDPELVGEVLKTMRGLAEQGMTMLVVTHEMGFARDVADDVI
                     FMDRGAIVEQSSPDRIFAEPQEARTRAFLERLLEREGGTRRKA"
BASE COUNT          610 a         1349 c         1346 g          612 t
ORIGIN      
        1 gtttccgcct tcggcagcgc acctgcgtcg cgcgagcggc cgatccagac gatccgcccg
       61 cccgcgacgg cgatggcggc gtcggagacg accgtgcccg catcgtccat ggtcacggcg
      121 tcgcagccgc gcacgaggac gtcgacgcgt tccggccggt tcgccggagc atcactcaag
      181 gacatcgcgg tactcctcgg tcagagaccc cgatgcgcct cgggaacgag gctgaaatcg
      241 accgcgcgat ccacatcggg cagcttggtg cgtccagtcg cccgcgcggc gcggaacatg
      301 ggcccttcga tcacctcccg atcaagcgcg aggcgcgggc gcggagcgcc ctcgtatcgg
      361 gtgagcggga tctgcagctc gttctggcga atctgctgtg ggagatcgag gccgaagtca
      421 gccccgtact tcttgacggc aagctcggcc gccactttcg gatctgcctc gttctgctcc
      481 caccccttga tgatcccgcg cacgtaggcg accacctgcg ggcggttggc gtccaggtag
      541 cggcgcgtcg tcatcagcac cgccccgtag gtgcggaagc cgagctcgtc gaagctgacg
      601 aagtggaaat ccttgccgcg cacgagcccc atgcgttcga gggtgatggt ctggttggtc
      661 ccgaaggcgg tgtagccgtc gccgtcgccc gcgagcagcg gctccggcga gaagccggcc
      721 ggcacttggg tccattggac cggcaggccg gcgagcgaga gagtcgcctc gatcgcggtc
      781 ttctcgttcg ggccctgggc gaggatgcgc gcgccgacta ggtccttcgg ttcccggatc
      841 ggcttcttgg caagagagat gatgccgagc gggttcttcg gaaacaccgc cgcgatcatc
      901 acgagatcgt tcccctgcgc gaccgcgtcg atgaacggga accaggtcga atagccaaga
      961 tcggcccggc cggccgcgag catcaccggc gccggcagcg cgttcggccc gccgggatac
     1021 cactcgacgt cgatgccctg tgctgtgagg aggcctcggt cgagggcgat ccattcgccg
     1081 gcgtactgga cgttgggaat ccagccgatc tggacccgca ccttcgggcg agtctgcgcg
     1141 agaagctcgc gtccgaacag gggcgccgcc actccggacg ccgctgccag tttgagaaaa
     1201 tcgcgacgcg cctgtctcat gggtccctcc tccggtcggg cggggaacaa gggttacagg
     1261 actaggctac caaatgtaac gtgggataca actgagatcg tgcaggaatt tttggcagag
     1321 cgacgcccat ggatgccgaa agattcagcg gaatggcggt gccggaacgc atccggcagg
     1381 gctatccggg cctgaccggc gcgttgcgcg ccttcgcgga tttcgtcctg agggagccgg
     1441 tgcgggccgc ccggatgagc atccatgccg cggtcgaagc ggccggggtg tcggtcgcca
     1501 ccggcaaccg cttcgcgcgg gcgatcggct tcgagaacta tcccgcgttc cgggcggacc
     1561 tgatcgagag cctcgcttcg gcttacgagc cggtgaagcg cctcgagcag cagatctcga
     1621 agcggtcgag cagtcacgac atcatggtgg ggtcgctgcg cgaggacgtg tcgaacctcg
     1681 aagagacggt cggcgggctc gacgaggcga cctgccggcg ggcggtcgag atgatcctcg
     1741 gggcccgacg gatcttcgtg ctcgggttcg acaacggggc ctgcctcgcg cagatcttgg
     1801 cgaacggcct gatgctcctc aaggacaacg tcgcccatgt ggcgaacggc ggcggcgggg
     1861 ccggagtcgc gcgccacctc tccggcttcg acggctccga cctcgtgatc gcgatcgcct
     1921 tccctcgcta catgaaggac acgatcagcc tcgccgggct ggcacgctcc cgcgaagtcc
     1981 ccgtcctcgc cattaccgac agccaccatt cgccgctcgc ggcgctcgcg accgtcagcc
     2041 tccatgcctg ctcgcgtcgg cagttcgcct ccgtttcgaa cgccgccgcc ctggcggtcg
     2101 tcgaagcgct gctcgccgcc gtggcgcacc gcaccccgaa atccgtccac cgcgccgaag
     2161 ccttcacggc ttgcgcgctg ccgtggatcg aagccgggcc ggcccggcga ccccgaagcg
     2221 attagtgcgc tcgggctccg tgccgatggc gagtcgccag ccctatgcct ccaggtccca
     2281 gaactcgcgg gcgatgacgc agcgggacac ggcctcggcc gcagcctgaa gcagcgcctc
     2341 gcctttggcg gcggaggcgc ggcgcggcac gccgatgacg ccgtgggagg tgcgcgcctc
     2401 gaacgagcgc catcgatgcg ctgcctccga cccgatcacg tcggcgacct cgcgcccggt
     2461 cggaccgacg gtttcggcgg ccgcgagcat gtcgacgcgc tcgggacgga tcgccagcat
     2521 catcgaggtt tccgcctcgc aggcgtgccg aacgttcggc tgatcctcca ggatcgcgcc
     2581 gaattccttc gcggcgagca gccaatacgt cgccgtcgcg atcggactgt cgagttcggg
     2641 ggcgatctcg ttcacgatca cggtgagggc ggcgatgttg ccgccgtggc cgttgaggag
     2701 gaggatgcgg ccgaacccct ggcgcacgag ggagcggcaa acgcccttca cgagggccga
     2761 gaaggtcgcg aaatcgagcg tgatcgtgcc gccgaggctc atgtggtgtt cggcgagacc
     2821 cgtccacaga accggcgtca ccaggaccgg gcactgggcc tcggcgatcc gggcggcccg
     2881 gcgggcgatc tcaccgacga gggcgcagtc gacctcggtg gtcaggtgag gcccgtgctg
     2941 ctctgtcgag gcgaccggca cgatcaccac cgtctccggc cgggatcgct cgcggatctc
     3001 ggcggctctc agcctccacc actcgacctc gctcatgcct tcctccgggt gccaccctcg
     3061 cgctccagca gccgctccag gaaggcgcgc gtccgcgctt cctgcggctc cgcgaagatg
     3121 cgatcgggtg aactctgctc gacgatcgcg ccgcggtcca tgaagatgac gtcgtcggcg
     3181 acgtcccggg cgaagcccat ctcgtgggtg acgacgagca tcgtcatccc ctgttcggcg
     3241 agcccccgca tcgtcttcag cacctcgccg acgagttccg gatcgagggc ggaggtgacc
     3301 tcgtcgaaca gcatgatccg cggctccatg gcaagcgcac gcgcgatcgc ggcccgctgc
     3361 tgctggcccc cggagagccg tccgggatag gcgtccttct tgtcctcgag gccaacgcgc
     3421 cggaggaggt cgacggcgat cgcctccgcc tgctcccggg agacgccgcg cacgcgttgc
     3481 ggggccaagg tgatgttccg caggacggtc agatgcggga acaagttgaa ctgctggaag
     3541 accatgccga cgtcgtgccg cttcgtcctg cgcggtgcgg atcgcccgtc cggctcttcc
     3601 tcgccaaccg ccacgagcga gtcggcgatc cagatgcgcc cgcgctggat ggcttcgagt
     3661 ccggcgatgc agcgcagcaa cgtgctcttg ccggagccgc tcggcccgat cacgacgagg
     3721 acctgggcgg gttcgacgct gagcgtcacg cggtcgagca cggtttccgg gccgaaccgc
     3781 ttcacgaccc cctggaggcg cacgagcgaa tccgtcatct cagagtcggc tcgccgcgaa
     3841 ccgccgctcg atccacgcga acaggagagc gagcgggtag gtcagaacga aataggcgac
     3901 cgccacgatg gtcaggg
//
LOCUS       DATLGG010000005         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_16007, whole genome shotgun sequence.
ACCESSION   DATLGG010000005 DATLGG010000000
VERSION     DATLGG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16007"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VM638_00100"
     CDS             <1..1582
                     /locus_tag="VM638_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVM10862.1"
                     /translation="MLEVPAAQGMDPSDGLLRIVTAHAREVEHVASLVLDRSAPGAEA
                     DPPIPHPLEDGAGDGPWTPEVREAFLAVLTDPAGGARTLETLDRLGRLVRLIPEWEAV
                     RCRPQRDPYHRFPVDTHLVRTAEGAARILTGRADDPMLQEAAQSVSDRNAVVLGAFLH
                     DIGKRGEGPHVPVGTRVATAVLHRMGIEGDTAERARFLVQEHLLLADTAVRRDLSDPS
                     VVDEVASRVGDRERLAMLHVVTAADARATGPLAWTPWRQALVRDLVARVDRLLLRRPD
                     PAGEMLAARKAGLRERLGPAAETFLRRVPRAHLVGTALADLLHHASMLERPLGRTEVR
                     TWARAGAARGVVALTVVAADRPGLLATIAGALSLGGLDIASAEVITTLDGVALDTFHV
                     ATPGGGDVDEARWRRFRTDLRRALEGRIDLGHRIRERRRHYPPPDPRIPVDVVVDNAA
                     SPGTTVLEVSAPDRVGLLFDLAEALHGLTLDVRVAKVATYGARVVDVFYVRDAAGRAI
                     EGAEAERVRRLVAEAARG"
     gene            1579..2223
                     /locus_tag="VM638_00105"
     CDS             1579..2223
                     /locus_tag="VM638_00105"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HVM10863.1"
                     /translation="MKRLSVRTPDASVSAVLSGDGEPLLVLAHGAGGSIDSPFLEGVA
                     EGLAEHEVASLRFNFPYREQGRGAPDREPVLRRTWAAVFEHAATLRRTVWAGGGSMGG
                     RIASMEVADGLPAAGLVLIAYPLHPPGRPERLRAAHLAGIEVPTIVIQGTRDAFARWD
                     LLTRAIERSGAPITLHVVEGADHSFRVPGTKRDDLAQGREIGRIAGGFVRAHAY"
BASE COUNT          274 a          807 c          843 g          307 t
ORIGIN      
        1 gatgctggaa gtgccggcgg cccaggggat ggacccgtcc gacggtctcc tgcggatcgt
       61 cacggcgcac gcccgcgagg tggagcacgt cgcatcgctc gtcctggacc gttcggctcc
      121 gggcgccgag gccgaccccc cgatcccgca ccccctcgag gatggcgcgg gtgacggacc
      181 gtggacgccc gaggttcggg aggcgttcct cgcggtgctc accgacccgg ccggaggagc
      241 gagaacgctc gagacgctcg atcgcctcgg gaggctggtg cgcctgatcc cggagtggga
      301 ggccgtccgg tgccggcccc agcgcgatcc ctaccatcgc ttcccggtcg acacccatct
      361 cgtccggacc gctgagggag ccgcgcggat cctcaccggc cgggccgacg atccgatgtt
      421 gcaggaggcg gcgcagagcg tctcggatcg gaacgcggtg gtgctcggcg cgttcctgca
      481 cgacatcggc aagcgaggtg aggggcccca cgtgccggtc gggacccggg tggcgacggc
      541 cgtccttcac cgtatgggga tcgaaggtga cacggccgag cgagcgcgct tcctcgtgca
      601 ggagcacctg ctgctcgccg acacggccgt ccggcgcgat ctctcggatc cctcggtcgt
      661 cgacgaggtg gcgagccggg tcggcgaccg cgagcgactc gcgatgctgc acgtcgtgac
      721 ggctgcggac gctcgtgcca ccgggcccct cgcgtggaca ccctggcggc aggccctcgt
      781 gcgcgacctc gtcgctcggg tggaccgtct cctgctgcgc cgcccggacc cggcgggcga
      841 gatgctcgcg gctcggaagg ccggcctgcg ggagcgcctg gggccggccg ccgagacgtt
      901 cctccggcgg gtcccccgag cgcatctcgt cggcaccgcg ctggctgacc tcctccacca
      961 cgcctcgatg ctcgaacgtc ccctcggccg cacggaggtg cggacctggg cacgcgccgg
     1021 cgcggcgcgg ggggtcgtcg cgctgacggt ggtcgccgcg gaccggccgg gactgctcgc
     1081 gacgatcgcg ggcgcgctct ccctcggcgg actcgacatc gcctccgccg aggtgatcac
     1141 cacgctggac ggggtggcgc tcgacacctt ccacgtcgcg acgcccggag gcggcgacgt
     1201 cgacgaggct cgctggcggc ggttccggac cgatctgcgg agagccctcg agggcaggat
     1261 cgacctcggc catcgcatcc gagagcggcg ccggcactac ccgccgccgg atccgcggat
     1321 cccggtggac gtcgtggtcg acaacgcggc ctccccggga acgaccgtgc tcgaggtgtc
     1381 ggccccggat cgcgtcggac tgctcttcga cctcgccgag gccctccacg ggctcactct
     1441 cgacgtgcgc gtcgcgaagg ttgcgacgta cggtgcacgg gtggtggacg tcttctacgt
     1501 cagggatgcg gccggccgcg cgatcgaggg agccgaggcc gagcgggttc gacgactggt
     1561 cgccgaggcg gcacgcggat gaagcgcctg tcggtccgga cgccggacgc ttccgtttcg
     1621 gccgtgctgt ccggcgacgg agagccgctc ctggtcctgg ctcacggggc gggagggtcg
     1681 atcgacagcc cgttcctcga gggggtcgcc gaaggcctcg ccgagcacga ggtggcgagc
     1741 ctccgcttca acttcccgta ccgcgagcag ggccggggcg cccccgaccg cgagccggtc
     1801 ctgcgtcgga cctgggcggc ggtcttcgag cacgccgcga cgctccggcg aaccgtgtgg
     1861 gcgggcggcg ggtcgatggg cgggcggatc gcgtcgatgg aggtggccga tggcctgccg
     1921 gccgcgggtc tcgtcctgat cgcctacccg ctgcacccgc ccggccgacc cgagcggctc
     1981 cgggccgcgc acctcgccgg catcgaggtg ccgacgatcg tgatccaggg aacccgcgac
     2041 gcgttcgctc gctgggacct gctcacccgg gcgatcgagc gctccggggc gccgatcacg
     2101 ctccacgtcg tcgagggggc ggaccactcg ttccgggttc cggggacgaa gcgcgacgac
     2161 ctggcgcagg gacgtgagat cggtaggatc gccggcggct tcgtccgcgc ccacgcatac
     2221 tgacggcatg c
//
LOCUS       DATLGG010000006         4456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_158478, whole genome shotgun sequence.
ACCESSION   DATLGG010000006 DATLGG010000000
VERSION     DATLGG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158478"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="cgtA"
                     /locus_tag="VM638_00110"
     CDS             <1..757
                     /gene="cgtA"
                     /locus_tag="VM638_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HVM10864.1"
                     /translation="PNAGKSTLLAALTAARPKVADYPFTTLSPNLGVAEDEDARYVLA
                     DVPGLIEGAHEGRGLGHRFLRHVSRCGGLAYVVDLSAEDPAADLRVIRAEVEAFDPAM
                     RTRPSIVIGTKADLVDEGLLAERAAALGPGSVVVSAVAGRGLDELAASLARLATRGAE
                     AVPREPYVVLRPARESFSVRREGGRFRVVGPSVERWVAEADLDDEASVARLQKRLKRG
                     GVERRLAEEGARPGDEVLIAGRAFEFHPDEEDG"
     gene            775..1860
                     /gene="proB"
                     /locus_tag="VM638_00115"
     CDS             775..1860
                     /gene="proB"
                     /locus_tag="VM638_00115"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HVM10865.1"
                     /translation="MRRLVVKVGSSSLVGPEGAVSRPRLSKVVRELAEVAAGRGCVLV
                     SSGAIATGLAPLGLGRRPRDMPSLQAAAAVGQGRLLAEYLRLFERRGLIAAQVLLTQD
                     DVVRRRHFVNARNTLERLLLGGVVPIVNENDTVGTEEIRFGDNDRLAAMVAVMIEADL
                     LVLLSDVDGIYTSDPRRGRGVLLSELRNPLAVDAGGSRSGLGSGGMASKVEAAAIASA
                     AGVGVVVAGARRRQILPRVLAGEELGTWVPPRSGRKRARKAWLAFASGPSGRIVVDAG
                     AERALRRDGRSLLAAGVVAAEGDFRAGQTVELVGPSGDAFGRGVANYSSEELPLLAGR
                     SSAELASLRGGPFEREVVHRDELVILD"
     gene            1873..3126
                     /locus_tag="VM638_00120"
     CDS             1873..3126
                     /locus_tag="VM638_00120"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HVM10866.1"
                     /translation="MRPMGALEPLVTPARMAARALATADREAKDACLAEMASALERRS
                     DEILWANREDVATAERDAAPGAVIDRLRLDEERVRAVAEGLRDIARLPDPVGEVIEER
                     TAPSGIRLQKVRVPLGVIGVIYEGRPNVTVDAAGLCLKAGSAVLLRGSRIARSSNEAL
                     VAVLRDAAEVADLPADVVQAVPPTRESAKEMMRARGLIDLLIPRGGNDLIQTVVNESV
                     VPVVETGIGNCHVYVDRAADLDRAQRILVNAKVQRPGVCNAAESFLVHRDVADAFLPG
                     ALEALRAEGVEVLGDEEVQARGEDVAPASDDDHRAEFLDLRISGKVVGSLDEAIEHIA
                     EYGTRHTEAIVTEDAEAAERFVREVDAAAVVVNASTRFTDGGEFGMGAEIGISTQKLH
                     ARGPMALSEITTYKWIVRGDGHVRG"
     gene            3204..3779
                     /gene="nadD"
                     /locus_tag="VM638_00125"
     CDS             3204..3779
                     /gene="nadD"
                     /locus_tag="VM638_00125"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HVM10867.1"
                     /translation="MGGTFDPIHIGHLVTAEGALATFELDAVVFVPTGQPWMKEGRDV
                     SPAEHRYLMTVIATASNPHFDVSRLEIDREGPTYAIDTLRSLRDHHGGETELFFITGA
                     DAVLEIFHWKDPEEVLALAHVIAATRPGYDIARFEAEAPTRHPRISVMTIPALAISST
                     DIRRRVRDAEPIRYLVPEGVSAYIEKWRLYR"
     gene            3776..>4456
                     /locus_tag="VM638_00130"
     CDS             3776..>4456
                     /locus_tag="VM638_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10868.1"
                     /translation="MRDAEDLLREVHDLLDGGVVQEPAAPAEDPPPRQPEREQVPVVP
                     RRAVAGRRHRPRRRAARVVLVIVVALAAVAAGVLTVRELRTSGPRNGDRPATAPAVPQ
                     DVLAWTVWTEQPTRAYVAVLATSEDLEPVALTVPGHLIVSIPGRGVGTIGDAAAGGNA
                     ELVGTTVSSVLGVEVDETVASSLEELGGIVDALGGIDIGEGRSSGARVVRYLRAKDRD
                     ADFRAIRWQ"
BASE COUNT          599 a         1526 c         1722 g          609 t
ORIGIN      
        1 gccgaacgcc ggcaagtcca cgctcctcgc ggcgctcacc gcggcccgtc cgaaggtggc
       61 ggactatccc ttcacgacgc tgtccccgaa cctcggcgtg gcggaggacg aagacgctcg
      121 ctacgtcctg gccgacgtcc cggggctgat cgagggcgcg cacgaaggac gcgggctcgg
      181 ccaccgcttc ctccgtcacg tctcacgctg cgggggactc gcctacgtcg tcgacctctc
      241 cgcggaggat cccgccgccg acctgcgggt gatccgcgcg gaggtcgagg cgttcgaccc
      301 cgccatgcgg accagaccct cgatcgtgat cgggacgaag gccgacctcg tcgatgaggg
      361 gttgctggcg gagcgtgccg ctgcgctggg gccgggctcc gtcgtcgtct ccgcggtggc
      421 tggccggggc ctggacgagc tcgccgcgag cctcgctcgt ctcgccacgc gcggggccga
      481 ggccgtcccg agggagccgt acgtggtcct ccggcccgcg cgcgagtcct tcagcgtgcg
      541 ccgggagggt ggccggttcc gagtcgtggg gccctccgtc gagcgttggg tcgccgaagc
      601 cgacctggac gatgaggcgt ccgtcgcacg cctgcagaag cgcctcaagc gcggtggggt
      661 cgagcgccgc ctcgcggagg agggggcccg gccgggcgac gaggtcctga tcgccgggcg
      721 ggcgttcgag ttccatcccg acgaggagga cggctgacgc gcgccctgcc ggagatccgg
      781 cgtctcgtcg tcaaggtcgg gagctccagc ctggtcggtc cggagggggc ggtctcgcgt
      841 cctcgcctgt cgaaggtcgt gcgcgagctt gcggaggtgg ctgccgggcg aggatgcgtc
      901 ctcgtgagct ccggcgcgat cgccacgggt ctcgcgccgc tcgggctcgg ccggcgaccg
      961 cgcgacatgc cgagcctgca ggccgcagcc gcggtggggc aggggcgcct cctggccgag
     1021 tacctgcggc tgttcgagcg ccgcggattg atcgccgccc aggtccttct gacccaggac
     1081 gacgtggttc ggcggcggca cttcgtcaac gcgcggaaca ccctggagcg gctcttgctc
     1141 ggcggcgtcg tcccgatcgt gaacgagaac gacaccgtcg ggaccgagga gatccgcttc
     1201 ggtgacaacg accggctcgc ggcgatggtg gccgtgatga tcgaggcgga cctgctcgtc
     1261 ctcctgtccg acgtcgacgg catctacacg agcgacccgc gccgaggccg gggggtcctg
     1321 ctgtcggagc tccggaaccc gctcgcggtc gatgcggggg ggagccggag cgggctcggc
     1381 tccggcggga tggcctccaa ggtggaggcg gcggcgatcg cctcggccgc aggcgtgggt
     1441 gtggtcgtcg cgggcgcacg ccggcgccag atcctgccgc gggtcctcgc cggggaagag
     1501 ctgggaacct gggtgccgcc gcggtccggc cggaagcgcg cgcggaaggc ctggctcgcg
     1561 ttcgcgagcg gccccagcgg ccgcatcgtg gtcgacgcgg gcgccgagcg tgccctccgg
     1621 cgcgacggcc ggagcctcct cgccgcgggc gtcgtcgcgg ccgagggtga cttccgcgcc
     1681 gggcagacgg tcgagctcgt cgggccgtcg ggcgacgcgt tcggacgtgg ggtggcgaac
     1741 tactcgagcg aggagcttcc gttgctcgcc gggaggtcgt cggcggagct cgcatccctg
     1801 cgaggcggtc ccttcgagcg cgaggtggtc caccgcgacg agctcgtcat cctcgactga
     1861 ggggggcgta cgatgaggcc gatgggagcg ctcgagccgc tcgtcacgcc cgcgcggatg
     1921 gcggcgcgcg cgctggccac cgccgatcgg gaggcgaagg acgcgtgtct cgccgagatg
     1981 gcatcggccc tcgagaggcg cagcgacgag atcctctggg cgaaccgcga ggatgtcgcg
     2041 acggccgagc gcgacgcggc gcccggcgcc gtcatcgacc ggctccgcct cgacgaggaa
     2101 cgcgtgcggg cggtcgccga gggcctgcgc gacatcgcgc ggctgccgga cccggtcggg
     2161 gaggtgatcg aggagcggac ggcgccgagc ggcatccgcc tccagaaggt gcgcgtgccg
     2221 ctcggggtga tcggcgtgat ctacgaaggg cggccgaacg tgaccgtgga cgcggcggga
     2281 ctctgcctca aggcgggcag cgccgtcctc ctccgggggt cgcgcatcgc ccgctcctcg
     2341 aacgaggccc tggtcgccgt cctgcgcgac gcggccgagg tcgcggatct gccggcggac
     2401 gtcgtccagg cggttcctcc gacccgtgag agcgcgaagg agatgatgcg cgcgcggggg
     2461 ctgatcgacc tcttgatccc tcgaggcggg aacgacctga tccaaacggt cgtgaacgag
     2521 tcggtcgtgc cggtcgtgga gaccggtatc gggaactgcc acgtgtacgt cgaccgcgcc
     2581 gcggacctgg accgtgcgca gcgcatcctc gtgaacgcga aggtgcagcg cccgggcgtg
     2641 tgcaacgctg cggagagctt cctcgtccac cgcgacgtgg ccgatgcgtt cctgccgggc
     2701 gcgctcgagg ccctgcgcgc ggagggggtc gaggtcctgg gagacgagga ggtgcaggcg
     2761 cgcggcgagg acgtcgctcc ggccagcgac gacgaccacc gcgccgagtt cctcgacctg
     2821 cggatcagcg ggaaggtcgt tgggtcgctc gacgaagcga tcgagcacat cgcggagtac
     2881 ggcacgcgcc acaccgaggc gatcgtgacg gaggatgccg aggcggccga gcggttcgtg
     2941 cgcgaggtcg acgcggcggc ggtcgtcgtg aacgcgtcga cgcgcttcac cgacggcggt
     3001 gagttcggca tgggggccga gatcgggatc tccacgcaga agctgcacgc ccgcggaccc
     3061 atggccctgt cggagatcac gacgtacaag tggatcgtcc gcggcgacgg tcacgtccgc
     3121 ggctgagctc gcgggaaatc cacggttggg cgggggcccg gacctaggat agaggcgtgg
     3181 agcctcccag ccgcctcggc gtcatgggcg ggaccttcga cccgatccac atcggccacc
     3241 tggtgacggc ggagggcgcc ctggcgacgt tcgagctcga tgcggtcgtg ttcgtcccga
     3301 ccggccagcc ctggatgaag gagggcaggg acgtctcgcc cgccgagcac cgctacctca
     3361 tgacggtcat cgcgaccgcc agcaacccgc acttcgacgt gtcgcggctc gagatcgatc
     3421 gcgaaggtcc gacgtatgcg atcgacacgc tgcggagtct ccgcgaccac cacgggggcg
     3481 agacggagct cttcttcatc acgggcgccg acgccgtgct cgagatcttc cactggaagg
     3541 atcccgagga ggtcctcgcc ctcgcccacg tcatcgccgc gacccgccct gggtacgaca
     3601 tcgcacgctt cgaagcggag gcgccgaccc gccatccgcg catctccgtg atgacgatcc
     3661 ccgcactcgc gatctcctcg accgacatcc ggcgacgcgt ccgcgacgcg gaaccgatcc
     3721 ggtacctggt cccggagggc gtcagcgcct acatcgagaa gtggaggctc taccgatgag
     3781 ggatgccgaa gatctgttgc gcgaggtgca cgacctcctc gacggcggcg tggtgcagga
     3841 gccggccgct cccgccgagg acccgcctcc gaggcaacct gagcgcgagc aggtgccggt
     3901 cgtcccgcgg cgggcggtcg cgggccgccg gcatcggccg cggcggcggg ccgcgcgcgt
     3961 cgtactggtg atcgtcgtcg cgctggcggc ggtcgccgcg ggcgtcctga ccgtccgcga
     4021 gctgcggacg agcggaccgc gcaacggcga caggccggcc acggctccgg ccgttccgca
     4081 ggacgtcctg gcgtggaccg tgtggaccga acagccgacg cgcgcctatg tggccgtcct
     4141 ggcgacgagc gaggacctcg aaccggtcgc cctcacggtg ccgggtcacc tgatcgtcag
     4201 catcccgggt cggggcgtcg ggacgatcgg cgatgcggcc gcgggcggga acgcggagct
     4261 ggtcggaacg accgtctcga gcgtcctcgg ggtcgaggtc gacgagaccg tcgcgagctc
     4321 gctcgaggag ctcgggggga tcgtcgacgc cctcggcggg atcgatatcg gggaaggtcg
     4381 atccagcggc gcgagggtgg tccgctacct gcgggcgaag gaccgcgatg ccgacttccg
     4441 cgcgatccgg tggcag
//
LOCUS       DATLGG010000007         5320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_190120, whole genome shotgun sequence.
ACCESSION   DATLGG010000007 DATLGG010000000
VERSION     DATLGG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190120"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VM638_00135"
     CDS             complement(<1..202)
                     /locus_tag="VM638_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVM10869.1"
                     /translation="MRVLDTFLDAMGNTPLVRLRSVTKGLRPTILAKLEMLNPGGSVK
                     DRIGLRMIEAAEREGKLRPGGTI"
     gene            complement(246..1307)
                     /locus_tag="VM638_00140"
     CDS             complement(246..1307)
                     /locus_tag="VM638_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVM10870.1"
                     /translation="MAGRFSILVLFLTLPLTLFPAPSQAAVGRESVVPCPESRQPCWP
                     AAFAISPDGTTIFYGERFTGRIVRVDVATGEERLWGRIRGLATDGEQGLLGLAVDPDW
                     PSARWVYAYYTKASPLSNRVIRARRVDGSLRKQHLATIPAASYHNGGAIHFGADRRLY
                     VVTGDTGRPGRAQRIGNRAGKVLRMNRDGSAPRTNPYEGKLTFSFGHRNSFGITHDPQ
                     TGRLWQTENGPECNDEVNRIIGGRNFGWGGSSRCPNTNASGPRPVPPRSLFASVVAPT
                     GAAFCEGCGLGSANEGTLLMASWNDRAIRRLELTSDRKNVVDRQTIYTNPRGILGMAR
                     GPDGRVYFSDERGIYRLVP"
     gene            complement(1309..2514)
                     /gene="hutI"
                     /locus_tag="VM638_00145"
     CDS             complement(1309..2514)
                     /gene="hutI"
                     /locus_tag="VM638_00145"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HVM10871.1"
                     /translation="MTSSGALAAPVVETGVPGTAAIQHAVVAWEAGRITYVGPRDGYP
                     GEVPFEAAGLVVPGFVDCHTHLPFVGWRDDEYEARLAGATYRDLHGRGGGIARSARML
                     AEASDEEVLAFSRGLTDEMLEHGTTAFEMKTGYGLSVEAELRQARLARRLAEEIPQTV
                     TVTLLAGHAVPEGSSREAWVERATNELIPRAAAEGLVDAVDVYVEDIAFTVEDLRRVA
                     EAAAVAGLPVRCHAEQLGRTGAAEAAAELGARSADHLNHLDAAGIEALARTATTAVLL
                     PASTLLLRAEPPPVAELVAREAEIALASDLNPGTSPVLSMPEVLALGGALYGLAPERM
                     LPAVTATPARVLGLDDRLGTIEVGKRADLLVLDTLGFGSVPYRPGHNPVRRVVVGGVL
                     REEGPWYRR"
     gene            2708..>5320
                     /locus_tag="VM638_00150"
     CDS             2708..>5320
                     /locus_tag="VM638_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM10872.1"
                     /translation="MREDVASGTAIVGRDAELATVVSLLEGGLEGPRAIVIDGEAGIG
                     KTTLLDELTRAAEGRSYRVLRCRPAEAEMSLPYVALGDLLALVGDEELLSLPDLQRQA
                     LEVALVRADGRLPADPLAVGMGLASLLMDLAEAGPLLVAVDDVQWLDADSARILEFAV
                     RRLRIPMLIALARRTEGEEEPVPLALDRWMPAEGIRRVRLGPLSLAAMHDVFNRRLGT
                     LFHRPTLVRLQELSGGNPFFGLAIAGELVRRGREPVAGERLPVPRTLQELVSARLEAL
                     SPAARQVLLVASALSRPAARVIEDALGGTIRIEDALIEVEEAGIVTVDDRGRIRFTHP
                     LLASAAYSSASPQRRRRLHGRLAEVATGEERARHLGLSVTGPDEQAAALIEAAGRAAE
                     RRGAQAAAAELYGSAHRLTPADRPERGGRRALLAASALFATGELQRAQALAEEAAGSA
                     GGNLRAEALLLLGRIAHVAGTDPVAVDLFERAASEASDDRRLRGRIHAHLSWSHPFDE
                     ARAVEHAEVAIGLLDPDEDPDLVAFTLLTKFFFDAQLGRGVDRRLLERGLELERREAE
                     ERTGPINHLPLIWFKSTDEFEAARSRFRDEEAWARLRGDEGSRPNRVAQLAEVELRAG
                     NWELAERYIEEACAGIGEAGLGGPLSMPLRIRAMIDAHRGRIDRARETLDALIEATEA
                     RGNVWWTALCLSTSGSVSLTAGEAEAADGAFTRMRALLDEMGVVDALAERSQPDHIEA
                     LLLLGAPERAADVLARLKERDRYLSRLWIRLALPRCRALIRASAGDLPAALEVVAEAV
                     EDPSAGELPFELARAFLVKGQLHRRTRQKGAADRALRSAAEGFERVGSPIWAARARDE
                     LGRVGLRPGSPSELT"
BASE COUNT          723 a         1901 c         1937 g          759 t
ORIGIN      
        1 tgatcgtccc gccgggcctg agcttcccct cgcgctccgc cgcctcgatc atccgcagcc
       61 cgatgcggtc cttgacgctg ccgcccgggt tcagcatctc gagcttcgcg aggatcgtgg
      121 gccgcagccc cttcgtcacg gaccgaaggc gaacgagcgg cgtgttcccc atcgcgtcga
      181 ggaaggtgtc gaggacccgc atcggcccat tgtgcgccac ggcccccggt cgcgtcgacg
      241 cggcctcagg ggacgagccg gtagatcccg cgctcgtcgc tgaagtagac ccggccgtcg
      301 gggccgcggg ccatgccgag gatgccgcga gggttcgtgt agatcgtctg gcgatccacg
      361 acgttcttcc gatccgaggt gagctccaac cgtcggatcg cacggtcgtt ccacgacgcc
      421 atcagcaggg tgccctcgtt cgccgacccg aggccgcacc cctcgcagaa ggcggcgccg
      481 gtgggcgcga cgacgctcgc gaacagcgag cgcggcggga cggggcgagg cccggaagcg
      541 ttcgtgttcg ggcaccgcga cgacccgccc cagccgaagt tccggccccc gatgatccga
      601 ttcacctcgt cgttgcactc gggcccgttc tccgtctgcc acaggcggcc ggtctgcggg
      661 tcgtgggtga tcccgaacga gttccggtgc ccgaacgaga aggtcagctt cccctcgtac
      721 ggattcgtcc ggggcgcgga cccgtcgcgg ttcatgcgca ggaccttgcc cgcgcggttc
      781 ccgatccgct gcgcccgacc aggacgaccg gtgtcgcccg tcaccacgta gaggcgccgg
      841 tccgcgccga aatggatcgc gcccccgttg tggtagctgg ccgcggggat cgtcgcgagg
      901 tgctgcttgc gcagcgatcc gtcgacccgg cgcgcgcgga tcacgcggtt ggagagcggc
      961 gacgccttcg tgtagtacgc gtacacccac cgagccgacg gccagtccgg gtccaccgcc
     1021 aggccgagca ggccctgctc gccgtccgtc gcgagaccgc ggatccggcc ccagagccgc
     1081 tcctctccgg tcgcgacatc gacgcggacg atgcgcccgg tgaaccgctc tccgtagaag
     1141 atcgtcgtcc cgtcggggct gatcgcgaag gccgccggcc agcacggctg tcgcgactcc
     1201 gggcagggca cgacggactc ccggccgacc gccgcctgcg acggcgccgg gaacagcgtg
     1261 agagggagcg tcaggaagag gacgaggatc gagaaccgtc ctgccacgtc agcgacggta
     1321 ccacgggccc tcttcccgta ggacgccccc gacgaccacc cgccgcacgg ggttgtgccc
     1381 ggggcggtag gggaccgaac cgaagccgag cgtgtcgagc accagcaggt cggcgcgctt
     1441 cccgacctcg atcgtgccga gccggtcgtc gaggccgagg acccgagcgg gcgtcgccgt
     1501 gaccgccggg agcatccgct ccggagcgag cccgtagagc gcgccgccga gcgcgaggac
     1561 ctcgggcatc gacaggaccg gagacgttcc ggggttcagg tccgaggcga gggcgatctc
     1621 ggcctcgcgt gcgacgagct cggccaccgg cggcggctcg gctcgcagca gcagcgtgga
     1681 ggcggggagc agcaccgccg tggtcgcggt ccgagccagt gcttcgatcc cggcggcatc
     1741 caggtggttc aggtggtcgg cgctgcgggc cccgagctcc gctgccgcct ccgccgctcc
     1801 cgtccggccg agctgctcgg cgtggcaccg cacgggcagc ccggccaccg ccgccgcctc
     1861 ggccacgcgc cggagatcct cgaccgtgaa cgcgatgtcc tcgacgtaga cgtcgaccgc
     1921 gtcgacgagc ccctccgccg ccgcccgcgg gatcagctcg ttcgtggcgc gctcgaccca
     1981 agcctcccgt gacgagccct cggggaccgc atggccggcc aacaacgtga ccgtgacggt
     2041 ctgcgggatc tcctcggcga gtcggcgggc gagccgcgcc tgccgtagct cggcctcgac
     2101 cgacagcccg tacccggtct tcatctcgaa ggccgtggtg ccgtgctcga gcatctcgtc
     2161 cgttagcccg cgcgagaagg ccaggacctc ctcatccgag gcctcggcga gcatccgggc
     2221 gctccgagcg atcccgccgc cgcgcccgtg caggtcgcgg taggtcgccc ccgccagccg
     2281 ggcctcgtac tcgtcgtccc gccagccgac gaaggggagg tgcgtgtggc agtcgacgaa
     2341 gccggggacg acgagcccgg cggcctcgaa cggcacctcc cccgggtagc cgtcgcgggg
     2401 cccgacgtag gtgatgcgcc cggcctccca cgcgacgacg gcgtgctgga tcgccgccgt
     2461 ccccgggacg cccgtctcga ccacgggcgc ggccagtgcc ccggagctag tcacggcgaa
     2521 ccatgccgag atgatccgtc cgggggctca acttccgcgg ccacgtgccg agaagataga
     2581 tgaagcagtg ccacgagagg gcgctcgggc gctgcatgtg ccaggagggg gattcgcggg
     2641 cagtccccct ccttggcttt tcctgggccg ggggtccggc tggcctccct atactggctg
     2701 cgaccgcatg cgtgaagacg tggcctccgg caccgccatc gtcggacgag acgcggagct
     2761 cgccacggtc gtgtcgctcc tcgagggcgg gctcgaaggc cctcgggcca tcgtcatcga
     2821 cggggaagcc gggatcggga agacgaccct gctcgatgag ctgactcgcg ccgcggaggg
     2881 acgctcctac cgagtgctcc gatgccggcc cgccgaggcc gagatgagcc tcccctacgt
     2941 tgcgctcggg gaccttctgg cgctcgtcgg cgacgaggag cttctctctc ttcccgatct
     3001 tcagcggcag gcgttggagg tggccctggt tcgcgcggac gggcggctcc cggccgatcc
     3061 cctcgcggtc gggatggggc tggcatcgct cctgatggat ctggctgagg cggggcccct
     3121 gctggtggcg gtcgatgacg tccagtggct cgacgcggac tccgcgcgca tcctggagtt
     3181 cgccgtgcgg cgtctccgca tccccatgct catcgctctc gcgcgtcgga ccgagggaga
     3241 ggaggagccg gtccccctcg ccctggaccg gtggatgccg gccgagggca tccgccgggt
     3301 gcggctcggg ccgctctcac tggcagccat gcacgatgtg ttcaaccgcc gcctgggaac
     3361 gctcttccat cgccccaccc tggtacgcct ccaggagctc tctggaggga accccttctt
     3421 cgggctcgcg atcgccggcg agctggtccg ccggggccga gagcctgtgg ccggggagcg
     3481 gctgccggtg ccgcggacgc tccaggagct cgtctcggcc aggctcgagg ccctctctcc
     3541 cgcggctcgc caggtcctgc tggtcgcgtc ggcgctctcc cgccccgcgg cccgggtcat
     3601 cgaggacgcg ctcgggggca cgatccggat cgaggatgcg ctgatcgagg tcgaggaagc
     3661 gggcatcgtc accgtggacg atcggggacg gatccgcttc acgcaccccc tcctggcctc
     3721 cgcggcgtat tcgtccgcat ccccgcagcg acgtcggcgc ctccacggcc gactcgccga
     3781 ggtcgctacc ggtgaggagc gggcgcgcca cctcggcctc agcgtcaccg gaccggacga
     3841 gcaggcagcg gccctgatcg aggctgcggg gagagcggcg gagcgccgcg gagcccaggc
     3901 ggcggcggcc gagctttacg ggtcggccca tcggctcacc cccgccgatc gcccggagcg
     3961 cgggggccgg cgcgcgctgc tggctgcgtc ggcgctgttc gctaccggcg agcttcaacg
     4021 cgctcaggcg ctggccgagg aggcggcggg gtccgctggc ggcaacctgc gtgcggaggc
     4081 gcttctcctg ctcggcagga tcgctcacgt cgccgggacg gacccggtgg cggtcgacct
     4141 cttcgagcga gccgcgtcgg aggctagcga cgaccgccgc ctccgcgggc gcatccacgc
     4201 ccacctgtcg tggagccatc cgttcgacga ggcgcgcgcc gtcgagcacg cggaggtcgc
     4261 catcggcctc ctggacccgg atgaggatcc cgatctggtt gcattcacgc tgttgacgaa
     4321 gttcttcttc gacgcccagc tcggccgagg agtggatcga cggctgttgg agcgagggct
     4381 cgagctcgaa cggagagaag ccgaggagcg aaccggaccc atcaaccacc ttcccctgat
     4441 ctggttcaag agcacggacg agttcgaggc cgcacggtcg cgcttccgtg acgaggaggc
     4501 gtgggcgagg ctgcgaggcg atgaggggtc acgacccaac cgggtagccc agctcgccga
     4561 ggtggagctc cgtgcgggga actgggagct ggccgagcga tacatcgagg aggcctgcgc
     4621 ggggatcggg gaggcggggc tcggaggacc gctgtcgatg ccccttcgca tccgagcgat
     4681 gatcgatgcc caccgcggac gcatcgaccg ggcccgggaa accctcgatg cgctgatcga
     4741 ggccacggag gcgaggggga acgtgtggtg gacggcgcta tgcctttcaa cgtcgggttc
     4801 agtctcgctg accgccggcg aggccgaagc tgccgacgga gccttcaccc gcatgcgcgc
     4861 gctcctggat gagatggggg tcgtggacgc ccttgcggag cgcagccagc ccgatcacat
     4921 cgaggctctc ttgctcctcg gggcgccgga acgggcagca gacgtgctcg caaggctgaa
     4981 ggagcgcgat cggtacctat cccgcctctg gatccggctt gccctgccca ggtgccgtgc
     5041 gttgatccgg gcgagcgctg gagatctccc ggccgcgctc gaggtggtcg cagaggcggt
     5101 ggaggacccg tcggcagggg agctcccgtt cgagctcgcg cgggcgttcc tggtcaaggg
     5161 gcagctccat cgccgcacga ggcagaaggg cgcagccgat cgggccctca ggtccgcggc
     5221 ggaggggttc gagcgagtcg gaagtcccat ctgggcggct cgtgcccgag acgagctcgg
     5281 tcgtgtgggg ctgcgcccgg gctccccctc ggagctgacc
//
LOCUS       DATLGG010000008        14715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_174463, whole genome shotgun sequence.
ACCESSION   DATLGG010000008 DATLGG010000000
VERSION     DATLGG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174463"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..285
                     /locus_tag="VM638_00155"
     CDS             70..285
                     /locus_tag="VM638_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10873.1"
                     /translation="MDCWHCRRTAVGTCRFCGRGLCENHVATKPFILEVFGRETTRAL
                     VVEEALFCGTCRPRPDPVDLPELDDPA"
     gene            378..506
                     /locus_tag="VM638_00160"
     CDS             378..506
                     /locus_tag="VM638_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10874.1"
                     /translation="MADKKITPKEPTAKPATEDDERSEATKVSRVKRVKRVKRAKR"
     gene            623..1291
                     /locus_tag="VM638_00165"
     CDS             623..1291
                     /locus_tag="VM638_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVM10875.1"
                     /translation="MRRTPLLGEVDDAAVEAFATSGVMQLFRKGTYLLHQGDEGTDVF
                     FLWRGRIELSSVSSTGHRQLHTRIDPPQFFGEFSVLGQMPRSVTALALEDSEVWVVPG
                     DRYLGFLETEPAASRAMLRVLARQIHAHEAFVEDLLHLDLKGRVAKRLLSLVTASFED
                     LPPDGAIVPSIVTHADLASLSGGSRENVTRILSEFQRRGLVARDGRRYVLRDIRGLAR
                     LAGL"
     gene            1317..2912
                     /locus_tag="VM638_00170"
     CDS             1317..2912
                     /locus_tag="VM638_00170"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HVM10876.1"
                     /translation="MRVALGQVNPTVGDLDGNVELLAGWAERAGAEGADLVCFPELSI
                     TGYPPEDLVLRPAFVEENQRALAELARRVAAGPAVLAGFVDRTERGIHNAAALLREGR
                     VEATYHKQRLPNYGVFDEQRTFVPGEEACHLRLADSDLGISICEDAWRPGGPFASYAG
                     RVQAIPNLNGSPYHRGKTRERLAICAERARETGAWILYVNCVGGQDELVFDGGSMVVS
                     PAGRVTARAPMFEEALLVVDLPDGGGPEVEWPEGAEEVYRALVLGLDDYVRKNGFAEV
                     VLGLSGGIDSALTAVLAADALGPAAVRALAMPSPYSSSESLEDARDVAERLGIRLDVI
                     PIDGAFAAYREALAQVFAGSEPGVAEENLQARIRGNLLMALSNRFGSMVLATGNKSEL
                     AVGYSTLYGDMAGGFSPLKDVPKTLVYELARWRNAQGSPPIPDRVLTKAPTAELRPDQ
                     KDTDSLPPYEVLDPIIQAYVEEDRSPEEIVADGADAATVQRVVAMIDRAEYKRRQGAP
                     GVKITPKAFGKDRRMPITNRYRV"
     gene            complement(2926..4374)
                     /locus_tag="VM638_00175"
     CDS             complement(2926..4374)
                     /locus_tag="VM638_00175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVM10877.1"
                     /translation="MRGGDMKITRSASAVTALAAALLLPSSVSAGGNADVRINQPGAG
                     ETLPVCNLQGDVGMARSGATVVATWFDAGRCEAKGRAPVPPLPDISFVGYAWSEDGGA
                     TWTDAGTIEPPAGTSAIGAPAIAAGPEGYFAVAFRAEIAGGSIIAVARSTDGGRSFAP
                     AGRASGPADDDIFRYRQAITVSGSDLYVAWEEHVHARRSETSQLLLSRSTDGGATFRP
                     PVAVAAPTAGSPSRPDLAVDPDDTLHVVWSQGGVVWTARSTDRASTFGPRSRVMDAPR
                     TGHQAACLIGGWQQVLNGNIVASQAPSLAVDVGGSSDPDHPAFNPRRGTLYVAVAGAP
                     RAGDEADILFTRSSDAGRTWSAPRLVNDDATATDQFHPQIEVGDSGRVGVTWYDRRRS
                     TADEPNWQIDVYGAVSSDGGESFSPNARITDVAHEPGSLFPFLDGADVCAYVERNAMV
                     PAGGGFLVAWSDNRSGTHEAPDLDVYADILGT"
     gene            complement(4604..5869)
                     /locus_tag="VM638_00180"
     CDS             complement(4604..5869)
                     /locus_tag="VM638_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10878.1"
                     /translation="MGREQVRRIQGCIAATVLAAALAGGVPAGAQEQGWRPTAGGWVS
                     GPVEYITTVAREVGTAVDAVLHERRLYVTTWRSFSIYDVSDPLAPRHLSTRQLGPSLY
                     NEQPQTNGEILLISRDARYAPAVPGLPPGTNGGALEIYDVRDADNPQRIGVYVTPPGQ
                     RDHLWTCVFDCAYAYSASGTILDLRDPTAPRVVGNWSQQAPYRPQRFHHVIEVAPGIV
                     MTGSLPSYVLDARVDPTAPTVLASVEPKTAAPMPGAVMPSPLPKPMEESLPARVDWPG
                     ATKGRLALISMETPFSGPCTEESGDFQTFLTTGWRQSGTFKAADSYDLRKNGTFTDGR
                     PPANLLGCSSYGMDVHPRFAQAGGSVALSFFEHGIRVLGVDARGQISERAGFLPHAGN
                     SARPVWVTDEILYVIDLHRGIDVLRVSGA"
     gene            5949..6623
                     /locus_tag="VM638_00185"
     CDS             5949..6623
                     /locus_tag="VM638_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10879.1"
                     /translation="MEAQEREVDPQERGVRSGVALMLAVAAVVAAALAGRASLRAESA
                     FDAWQQAGRAEVKHGAALVEDVRFVYADEGPSALRALEARVRAEELREAARRSSGRVR
                     ATLLLEARTAEALANALEESGFDLGREAYRNPDGGLAIHRRLADVRGEDPALVALDPE
                     AHQARGNRAGDRAVLGLAATIPVGVAFLFGALGQALPRRRRGLVLAGWGCIAGALVIA
                     VLLEVL"
     gene            6623..7291
                     /locus_tag="VM638_00190"
     CDS             6623..7291
                     /locus_tag="VM638_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10880.1"
                     /translation="MDLDLELAHRESRTRFDVRLAALVGSAAIAAALLGTLEMHSGKR
                     EERAFTMASRLAVQVSSGTAVSGLRSSLQASAITQGAAGGLESAGRAIAAIEAGSPEG
                     GAIAAAEERASERLLELAQALLEAPGPDTGLDARTRGALEATPETLTLLVAEQNRQVD
                     LAERYGNRENAAIFGLSLVAIAAVLLGLAGILGEGRQGRMILTVATVVLGASVLWGGW
                     ALLR"
     gene            7322..7394
                     /locus_tag="VM638_00195"
     tRNA            7322..7394
                     /locus_tag="VM638_00195"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7356..7358,aa:Glu,seq:ttc)
     gene            7424..7498
                     /locus_tag="VM638_00200"
     tRNA            7424..7498
                     /locus_tag="VM638_00200"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7459..7461,aa:Asp,seq:gtc)
     gene            7516..7588
                     /locus_tag="VM638_00205"
     tRNA            7516..7588
                     /locus_tag="VM638_00205"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7549..7551,aa:Phe,seq:gaa)
     gene            complement(7648..7836)
                     /locus_tag="VM638_00210"
     CDS             complement(7648..7836)
                     /locus_tag="VM638_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HVM10881.1"
                     /translation="MTPQKPGEKLLTPAEVAALFRVDPKTVTRWAKAGKLSSIRTLGG
                     HRRYWENEVRSLLEDSGA"
     gene            complement(7972..8778)
                     /locus_tag="VM638_00215"
     CDS             complement(7972..8778)
                     /locus_tag="VM638_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10882.1"
                     /translation="MIATAYLRVFRPLAVMPEGERSHWEAYIAAGEHERPARPRYRHG
                     WTHGRLGIITSEEEGRADVRLIDGEWYVCPWRTRIRTLASLLSLRETIPREVADELVP
                     EVEARRAARELARIRRRDPAAIPTLLQSAWHVPVRWFVLVADEERRIVPRGPDRYRLS
                     YWTTLPEARTRTMRALEVLRRSELDVVADLVEDMASWLSVFPDRAAVELDYDRVSDLF
                     GPLELDEDHSARDLHASLDALEAGDPDRAGAMYERVAMRWAEAKTRESLN"
     gene            8857..9951
                     /locus_tag="VM638_00220"
     CDS             8857..9951
                     /locus_tag="VM638_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HVM10883.1"
                     /translation="MGVFEQLATEYEEVVFFHDAPSGLRAIVAIHSTQLGPALGGARF
                     YPFATEEEALRDVLRLAKGMTYKAAAAGLDLGGGKSVIIGDPRRDKSEELLRAFARMV
                     DTLGGRYITAEDVGTTREDMDVVRRETRWVTGVSKRLGGSGDPSPVTAYGVYNALRAC
                     AEEAFSAASLEGLHVVVQGVGKVGYHVAKHCVDAGARVTVADVDLDAVARAVKDLGAE
                     TVDSDKAHALECDIFAPCALGGVIRDDTIPELKCRVIAGAANNQLDRPELGETLADAG
                     ILYAPDFIVNAGGLINVADELMGYDRERAMKQVEDIFRTLREVFRISKKEDVTPARAA
                     ELVAEERIRSVSRLRKLYVPGERGHGTLRR"
     gene            10002..11069
                     /gene="pdhA"
                     /locus_tag="VM638_00225"
     CDS             10002..11069
                     /gene="pdhA"
                     /locus_tag="VM638_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HVM10884.1"
                     /translation="MTDDTVRILNPDGGVDPQARQALDLKDEDLLELYRSMVLTRNVD
                     LECTALQRQGELAVYPPAIGQEAAQVGSAFALGDQDMVYPSYREVGVAIVRGLDIVEY
                     MHFHRGTWHGGVDDPVDRQFGMISVPVGSQTLHATGHAMGTKLDGKDVVTITYFGDGA
                     TSQGDVHEAMNFAGVFGAPVVFFCQNNQWAISVPLERQTAGEIYRKAEGYGFPGVRVD
                     GNDVLAVYRVTREAVERARNEHQPTLIEAVTYRMGPHSTADDATRYREKADVDRWQEL
                     DPIARYRTFLEAEGIADQSFFLEAEADGKELAATVRAGIQGAPPRQVEDLFRWVFADL
                     PPHLARQEEEMLRLAKEEEDG"
     gene            11062..12036
                     /locus_tag="VM638_00230"
     CDS             11062..12036
                     /locus_tag="VM638_00230"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HVM10885.1"
                     /translation="MAELTMAQALNRALGDALAEDDRVLILGEDVGKLGGVFRITDGL
                     QDRFGTSRVFDTPLAESAIAGVSVGLSQAGWKPVCEMQFEAFSYPALDEVISHIAKYR
                     YRSKGTTQVPMVMRIPYGGGIGAAEHHSESPETYYAHTAGLKVVVPSTPLDAYTLLRR
                     AIDDPDPVIFFEPKSRYWSKEPGEPQMEGPGIGEARVAREGSHATLIAWGAMVTRCLE
                     AAAAAGEDGVEVEVLDLRSLVPLDLHALATSVQKTGRAVVVHEAPMTAGFGAEVVSRI
                     VEDSFDHLEAPPLRVTGYDTPYPPATLEEHYLPSVDRILLALEKVLTH"
     gene            12036..13370
                     /locus_tag="VM638_00235"
     CDS             12036..13370
                     /locus_tag="VM638_00235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HVM10886.1"
                     /translation="MAERTFNLPDLGEGLEEAEIVEWKVSAGDTVELNQPLVDVNTAK
                     ALVEIPSPWAGTIVKTYGSDGEVLKVGEPLVTIDVGGEAEAGAAPPTDEPKKREAVLV
                     GYGVDQETKSARRRRLRPPGPRQAPAAASAVSADTGAAYTDVEPQPSPPAGRALAAPP
                     VRKLAKELGVDLSTIAGSGPNGRVRREDVEAAAGRGTAPPTPLRGIPITISTGSETRV
                     PLRGVRRLIAEKMARSWAEVPHVTTFHVADATNVEALRRELTEEAGAKVTALAVVVRA
                     FAEICTKHPELNARHDPEANELVLHGSKHVGIATDTDEGLVVPVVRDVQAKGIVTVAR
                     EIAGLVEAARTRRATPDQLTGGTVTVTNVGTFGSDFGTPIINVPEVAILALGTIKHRP
                     LVVDGSVVARPTVTLSLSFDHRFIDGAHADRAMTDLRKLLESPFKLGALPRG"
     gene            13367..14281
                     /locus_tag="VM638_00240"
     CDS             13367..14281
                     /locus_tag="VM638_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HVM10887.1"
                     /translation="MKIVSLLPSATEIVFALGLGDNLEGVTYECDHPDEARTKPVVVE
                     GALPQDRPLSAREIEDAVREHMDAREPIYTLHKDLIQKIQPDLILAQDLCRVCAVPSG
                     QVEDALAELGTEAEVLSLDPHDLEGILASIVRVGRATGTEERAAEVVEGLRQRLEALR
                     ATAVRLPTIRTLALEWSDPPYVGGHWVPEMVQLVGGENLLNAPKERSRVVTWRQVADA
                     QPEVIVFMPCGYYLEEAEAEGARLFENPELRATTAVASGAVFAVDATSYFSRPGPRIV
                     DGAEILSWAIHPEAFPEPDLDRIVRITP"
     gene            14420..>14715
                     /locus_tag="VM638_00245"
     CDS             14420..>14715
                     /locus_tag="VM638_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM10888.1"
                     /translation="MALATIVAASLLGTLAPAAQAASRPTCLGKPATIVAKRNQTVVK
                     GTPKADVIVGSSIGQTIYGYGGNDRICAGGGNDKVYGGAGADRLHGGSGNDRLYG"
BASE COUNT         2191 a         5203 c         5267 g         2054 t
ORIGIN      
        1 cgttcgggcg gggaacgtcc gacccgaccg cccccgtcga ccgggacaag ccgcactgag
       61 aggagcgaga tggattgctg gcactgcagg agaacggccg tcggcacctg ccggttctgc
      121 ggccgggggc tgtgcgagaa ccacgtcgcg acgaagccgt tcatcctcga ggtcttcgga
      181 cgcgagacga cccgcgcgct cgtcgtggag gaggcgctct tctgcggcac gtgccggccc
      241 cggccggacc cggtcgacct gccggagctg gacgatccgg cgtgacgaac gtcacatatc
      301 accgcgtgac gagcgacaca gccggccgcg ggccccgggt gccatgatga acgcgcccga
      361 tacgaaggag gacgcccatg gcagacaaga agatcacccc gaaggagccc accgcgaagc
      421 ccgcgaccga ggacgacgag cggtccgagg cgacgaaggt ctcccgcgtg aagcgggtca
      481 agcgggtcaa gcgcgcgaag cgctagacct tccgcacctg atcgaacggc gagccccacg
      541 gggctcgccg ttccacgtcc gccccactcc ggggacgcgg tagcctcacg ccgtgcgccc
      601 gacccccgat gtcgcccgag cgatgcgacg aacccccctc ctcggggagg tcgatgacgc
      661 tgccgtcgag gcgttcgcca cctccggggt gatgcagctg ttccgcaagg ggacctacct
      721 gctgcaccag ggtgacgagg gcaccgacgt cttcttcctc tggaggggcc gcatcgagct
      781 cagctccgtc tccagcaccg ggcaccgaca gctccacacg cggatcgatc ccccgcagtt
      841 cttcggcgag ttcagcgtcc tcggccagat gccccgaagc gtcacggcgc tggcgctcga
      901 ggactcggag gtttgggtgg tccccggcga ccggtacctc gggttcctcg agaccgagcc
      961 ggctgcctcg cgcgcgatgc tgcgcgtgct cgcccgccag atccacgcgc acgaagcttt
     1021 cgtcgaagac ctcctccacc tcgacctcaa gggccgggtc gccaagcgcc tcctcagcct
     1081 cgtgaccgcg agcttcgagg acctcccccc cgacggggcg atcgtcccct cgatcgtcac
     1141 ccacgccgac ctcgcgagcc tctcgggcgg gagccgggag aacgtcacgc ggatcctgtc
     1201 ggagttccag cggcgtggac tcgtcgcgcg cgacggccgg cgctacgtcc tgcgcgacat
     1261 ccgcggactc gcccgcctcg ccgggctctg aaccgctccg gcggtacgct tcggacgtga
     1321 gggtcgcact cggccaggtg aatccgaccg tgggggacct cgacggcaac gtcgagctcc
     1381 tcgcggggtg ggcggagcgt gccggagcgg agggcgccga cctcgtctgc ttcccggagc
     1441 tctcgatcac cggctatccc cccgaggacc ttgtgctgcg gccggcgttc gtggaggaga
     1501 accaaagggc tctggcggag ctggcgcggc gcgtcgccgc ggggccggcg gtcctcgcag
     1561 ggttcgtcga ccggacggag cgcggcatcc acaacgccgc cgcgctcctg cgagagggcc
     1621 gggtcgaggc gacgtaccac aagcagcgcc tcccgaacta cggcgtgttc gacgagcagc
     1681 ggacgttcgt cccgggcgag gaggcgtgcc acctgcggct cgcggactcc gacctcggca
     1741 tctcgatctg cgaggacgcc tggcgtcccg gcggtccgtt cgcgtcgtac gcgggacggg
     1801 tccaggcgat cccgaacctc aacggctccc cgtaccaccg cggcaagacg cgggagcgtc
     1861 tcgcgatctg cgccgagcgc gcgcgggaga ccggcgcatg gatcctgtac gtcaactgcg
     1921 tcggcggaca ggacgagctc gtcttcgacg gcgggagcat ggtggtctcc cccgcgggac
     1981 gggtcacggc gcgcgcgccg atgttcgagg aggccctgct cgtggtcgac cttccggacg
     2041 gcgggggccc ggaggtcgag tggcccgagg gagccgagga ggtctaccga gcgctcgtcc
     2101 tcgggctcga cgactacgtc cggaagaacg gcttcgccga ggtcgtcctg ggtctgtcgg
     2161 gcggcatcga ctccgccctc accgcggtcc tcgccgccga cgccctggga ccggcagcgg
     2221 tccgcgcgct cgcgatgccc tcgccgtact cgtcgagcga gagcctcgag gatgcgcggg
     2281 acgtcgcgga acgtctcggc atccgactcg acgtgatccc gatcgacgga gcgttcgcgg
     2341 cgtatcgcga ggcgctcgcg caggtcttcg ccggatccga gcccggcgtg gccgaggaga
     2401 acctccaggc gcggatccgc ggcaacctgc tgatggctct gtcgaaccgc ttcgggtcga
     2461 tggtcctggc gaccgggaac aagagcgagc tcgcggttgg ctactcgacg ctctacgggg
     2521 acatggccgg ggggttctcg cccctgaagg acgtgccgaa gaccctcgtc tacgagctcg
     2581 cccgctggcg gaacgcgcag gggtcgccgc cgatcccgga ccgcgtgctg acgaaggcgc
     2641 cgacggccga gctccgtccc gaccagaagg acacggactc gttgccgccg tacgaggtcc
     2701 tcgatccgat catccaggcc tacgtcgagg aggaccgctc accggaggag atcgtggcgg
     2761 acggcgccga cgccgcgacc gtgcagcggg tcgttgcgat gatcgaccgc gccgagtaca
     2821 aacggcggca gggtgccccc ggcgtgaaga tcacgccgaa ggcgttcggc aaggaccgac
     2881 ggatgccgat cacgaaccgc taccgggtct gacgctcccg gaggctcagg tcccgaggat
     2941 gtccgcgtag acgtcgaggt ccggcgcttc atgggtgccc gatcggttat cgctccacgc
     3001 gacgaggaac ccgccgccgg ccggcaccat cgcgttgcgc tcgacgtagg cacagacgtc
     3061 cgcaccatcc aggaagggga agagcgaccc gggctcgtgg gcgacgtcgg tgatccgcgc
     3121 gttgggcgag aagctctcac cgccgtcgct ggagaccgcg ccgtagacat cgatctgcca
     3181 gttcggctca tccgcggtgg agcggcgacg gtcgtaccac gtcacaccga cacgtccgct
     3241 gtcccccacc tcgatctggg ggtggaactg atccgtcgcc gtggcgtcgt cgttcacgag
     3301 ccgcggcgcc gaccacgttc gcccggcgtc gctcgatcgc gtgaacagga tgtcggcctc
     3361 gtcgcccgca cgaggagcac cggcgaccgc cacgtagagc gtccctcgcc ggggattgaa
     3421 ggctggatga tccgggtccg acgacccccc cacatccacc gcgagggagg gagcctggga
     3481 agcgacgatg ttcccgttca gcacctgctg ccagccgccg atgagacacg cagcctggtg
     3541 acccgtacgt ggcgcgtcca tcacacgcga acggggcccg aaggtcgacg cccggtccgt
     3601 cgaccgggcc gtccagacga ccccgccctg ggaccagacc acgtggaggg tgtcgtctgg
     3661 atccaccgcc aggtcgggac ggctggggct tccagcggtc ggggcggcga cggcgaccgg
     3721 cggccggaag gttgcgccac cgtcggtcga gcggctcaac aggagctggg aggtctcgga
     3781 gcggcgcgca tgaacgtgct cttcccacgc cacgtacagg tcggagccgc tgacggtgat
     3841 ggcctggcgg taccggaaga tgtcgtcgtc agcggggccg ctcgcccgcc cagcgggggc
     3901 gaaggatcgg cctccatccg tggaccgcgc gacggcgatg atcgacccgc ccgcgatctc
     3961 ggcacggaag gccacggcga agtacccctc gggaccggcg gcgatggccg gcgcgccgat
     4021 ggcggatgtg ccggccggcg gttcgatcgt cccggcatcg gtccacgtcg cgccaccgtc
     4081 ctccgaccac gcgtagccga cgaacgagat gtccggcagt ggcggcacgg gggctcgacc
     4141 tttggcctcg catcgaccgg catcgaacca ggtcgcgacg accgtcgctc ccgagcgagc
     4201 catcccgacg tcgccctgca ggttgcacac cggcagggtc tcacccgctc cgggctgatt
     4261 gatccggacg tccgcgttcc ctcctgcgga cacggaagac ggcagcagga gcgccgcagc
     4321 cagcgccgtc accgcggagg cacttctcgt gatcttcatg tctccccctc gcacgtcgtc
     4381 acccgggact tcggcgagag gatgaccgag ccctccgcgc cccgcggcgg tccgaccgcc
     4441 acggctgcca tccagcgaac ccgccggggc cgcgctacga tcgtgcacgg gcgttagctc
     4501 agcctggtta gagcagtggg cttttaaccc atgtgtcggg ggttcgaatc cctcacgccc
     4561 caccacccga ccgggcgcga cggatgcctc gctgctgcgc ccactaggcc cccgacaccc
     4621 ggaggacgtc gatcccccgg tggaggtcga tcacgtacag gatctcatcg gtcacccaga
     4681 ccggccgcgc gctgttgccc gcgtgcggga ggaagccggc ccgctcgctg atctgtcccc
     4741 gggcgtccac cccgagcacg cggatcccat gttcgaagaa cgacagcgcg acgcttccgc
     4801 cggcctgcgc gaaacgcgga tggacgtcca tcccgtagga cgagcagccg agcaggttcg
     4861 cgggtgggcg gccgtcggtg aacgtgccgt tcttgcgcag gtcgtagctg tcggccgcct
     4921 tgaacgtccc gctctggcgc cagccggtcg tcaggaaggt ctggaagtcg cccgactcct
     4981 cggtgcaggg accgctgaac ggggtttcca tcgagatcag cgccaagcgg cccttcgtgg
     5041 cgccgggcca atcgacgcgc gcggggagcg attcctccat cggcttcggc agggggctcg
     5101 gcatcaccgc gccgggcatg ggcgctgccg tcttcggctc gaccgaggcg agcacggtcg
     5161 gcgcggtggg atcgacccgc gcgtccagca cgtacgacgg gagcgacccc gtcatcacga
     5221 tgcccggcgc cacctcgatc acgtggtgga accgctgggg acggtaggga gcctgctggg
     5281 accagttccc gacgaccctg ggcgcggtcg ggtcacgcag atcgaggatc gtcccgctgg
     5341 cgctgtacgc gtacgcgcag tcgaagacgc aggtccacag gtgatcgcgc tgacccggtg
     5401 gggtcacgta cacgccgatc cgctgcgggt tgtccgcgtc gcggacgtcg tagatctcga
     5461 gcgctccgcc gttcgtcccg ggcggcagac cgggaacggc cggcgcgtag cgcgcatcgc
     5521 ggctgatgag gaggatctct ccgttcgtct gcggctgctc gttgtacagg ctcggcccga
     5581 gctgccgcgt ggacaggtgg cgcggcgcca gggggtcgga gacgtcgtag atcgagaacg
     5641 atcgccaggt cgtcacgtag aggcggcgct cgtgcaggac ggcgtcgacc gccgttccca
     5701 cctcacgcgc aacggtcgtg atgtattcga ccgggcccga cacccatcca cctgccgtcg
     5761 gacgccatcc ctgttcctgc gcaccagctg gaacgccgcc cgcgagcgcc gcggccagca
     5821 ccgttgccgc gatgcaaccc tggatcctcc gcacctgctc cctccccatc gatcggactc
     5881 cagggcttct tcgacggtcg aggcgggaaa tcctgccaac ccgcccggac ccgtataccg
     5941 tggcggccgt ggaagcccaa gagcgagagg tcgacccgca ggaacgaggc gtccgctccg
     6001 gcgtcgcgct gatgctcgcg gtcgcggcgg tcgtcgcagc ggcgctcgcg gggcgcgcgt
     6061 cgctgcgggc cgagagcgcc ttcgacgcgt ggcagcaggc cggccgagcg gaggtgaagc
     6121 acggcgcggc gctcgtcgag gatgttcggt tcgtgtacgc ggacgaggga ccgtcggcgc
     6181 tccgggccct cgaggcgagg gtccgggccg aggagctgcg cgaggccgcc cgtcggtcct
     6241 cgggacgggt ccgggcgacc ctgctcctcg aagcacggac cgccgaggcg ctggccaacg
     6301 ccctggagga gagcggcttc gacctcggcc gcgaggcgta tcggaacccg gacggcggcc
     6361 tcgcgatcca ccggcggctc gcggacgtgc gaggggagga tcccgccctc gtcgccctcg
     6421 accccgaggc gcaccaggcg cgtgggaacc gcgccggcga ccgagccgtc ctcgggctcg
     6481 ccgcgacgat ccccgtcggc gttgcgttcc tgttcggcgc cctcggtcag gccctccccc
     6541 ggcgacgccg cggcctcgtg ctcgcgggct ggggatgcat cgccggcgcg ctcgtgatcg
     6601 ccgtcctcct ggaggtgctc tgatggatct ggacctcgag ctcgcgcacc gggagagccg
     6661 cacgcgcttc gacgtccgcc tcgcggcgct ggtgggctcg gcggccatcg ccgccgctct
     6721 gctggggacc ctcgagatgc actccggcaa gcgcgaggag cgggcgttca cgatggcgtc
     6781 gcggctggcc gtgcaggtgt cgagcgggac cgccgtgagc ggtctgcgca gctccctgca
     6841 ggcttccgcg atcacgcagg gcgcggcggg cggactcgag tcggccggcc gagcgatcgc
     6901 ggcgatcgag gcgggctccc cggagggcgg ggcgatcgcc gcggcggagg agcgcgcgtc
     6961 ggagcgcctg ctcgagctgg ctcaggccct cctggaggcg ccgggaccgg acaccgggct
     7021 cgacgcgcgg acgcgcggtg ccctcgaagc cacccccgag acgctgacgc tcctcgttgc
     7081 ggagcagaac cgtcaggtgg acctggccga gcgctacggg aaccgcgaga acgccgcgat
     7141 cttcggactg tccctggtcg cgatcgccgc cgtcctcctc ggcctcgccg gcatcctcgg
     7201 cgaggggcga caggggcgga tgatcctcac cgtcgcgacg gtcgtcctcg gagcgtccgt
     7261 gctctggggt ggatgggccc tgctgcggtg acggctatac tcgtgctcgc cccttggggc
     7321 tgcccccatc gtctagcggc ctaggatccc gccctttcaa ggcggcggca cgggttcgaa
     7381 tcccgttggg ggcacgcgac gcggtatcat ggttcggtta cctgggcctg tggagcagtc
     7441 tggagtgctc gccaccctgt caaggtggag gtcgggggtt caagtcccct caggcccgct
     7501 ctcgttcgga gcagcggcga ggtagctcag ttggcagagc actcggctga aaaccggggt
     7561 gtcggcggtt cgaatccgtc cctcgccacc gaatggaaga agcccggccg ctcggccggg
     7621 cttccgtggt tctgatcccc gggggtgcta cgcgccggag tcttcgagca ggctgcggac
     7681 ctcgttctcc cagtagcggc ggtggccgcc gagggtgcgg atcgaggaca gcttgccggc
     7741 cttggcccac cgggtcacgg tcttcggatc cacgcggaac agggccgcga cctccgccgg
     7801 cgtgaggagc ttctccccag gcttctgcgg cgtcatcgtg ctcatgtcgt gccctccctt
     7861 cgcctgatcc ttcatgtgca cattgtcgga tgcccgtttc acccggtaca tagacaatgg
     7921 gggtggaaat cccgtagccc ggaataacta gttcggacgg gtccgtcccc gctagttcag
     7981 ggactcgcgc gtcttcgcct cggcccagcg catcgccacg cgctcgtaca tcgcgccggc
     8041 gcggtcgggg tctcccgcct cgagcgcgtc gagcgaggcg tggaggtcgc gggcggagtg
     8101 gtcctcgtcg agctcgagcg gtccgaacag gtcggagacc ctgtcgtagt cgagctcgac
     8161 ggcggctcga tcggggaaga ccgagagcca cgacgccatg tcctcgacga ggtcggcgac
     8221 gacgtcgagc tcgctgcggc gcagcacctc gagggcccgc atcgtgcgcg tccgagcctc
     8281 gggcagggtc gtccagtagg agaggcggta gcggtcgggc ccccgcggga cgatccggcg
     8341 ctcctcgtcc gccacgagca cgaaccagcg gaccgggacg tgccacgcgc tctgcagcaa
     8401 cgtcgggatc gcggcgggat cgcgccgacg gatccgggcg agctcgcgtg cggctcgccg
     8461 ggcctcgacc tcggggacga gctcgtcggc gacctcgcgc gggatcgtct cgcgcagcga
     8521 cagcaggctg gcgagggtcc ggatgcgggt ccgccagggg cagacgtacc actccccgtc
     8581 gatcaagcgg acgtcggccc gcccttcctc ctcgctcgtg atgatgccga gacggccgtg
     8641 cgtccaaccg tggcggtagc gaggacgggc ggggcgctcg tgctcccccg cggcgatgta
     8701 ggcctcccag tgggagcgct ccccctccgg catgaccgcg aggggacgga agacccgcag
     8761 gtaggccgtc gcgatcaccg ttggcccatg atagccggac ggcgtcggga gccgcttgct
     8821 atagtcgtcg cccgggcgag ttgcccggtt caccccatgg gcgtattcga gcagctcgcg
     8881 acggagtacg aggaggtcgt cttcttccac gacgctccgt cggggctccg cgcgatcgtc
     8941 gcgatccact caacccagct cggcccagcc ctcggcggcg ctcgcttcta cccgttcgcg
     9001 accgaggagg aggcgctgcg tgacgtcctt cgcctcgcga aggggatgac ctacaaggcg
     9061 gccgcggcgg ggctcgacct cggcggcggc aagtccgtga tcatcggcga cccacggcgc
     9121 gacaaatccg aggagctgct gcgggcgttc gcgcggatgg tcgacacgct cggcggccgc
     9181 tacatcaccg ccgaggacgt ggggacgacc cgcgaggaca tggacgtcgt tcgccgcgag
     9241 acgcgatggg tcaccggagt ctcgaagcgc ctcggcggct ccggcgatcc ctccccggtc
     9301 accgcctacg gcgtctacaa cgcgctccgc gcctgcgccg aggaagcgtt ctcggcggcc
     9361 tccctcgagg gcctgcacgt cgtcgtccaa ggcgtcggga aggtgggcta ccacgtggcg
     9421 aagcactgcg tcgacgccgg ggcgcgggtg acggtcgccg acgtcgacct cgacgcggtc
     9481 gcgcgcgcgg tgaaggacct cggcgcggag accgtcgact ccgacaaggc gcacgccctc
     9541 gagtgcgaca tcttcgcgcc gtgcgccctc ggcggcgtga tccgcgacga cacgatcccc
     9601 gagctgaagt gccgagtgat cgccggcgcc gcgaacaacc agctcgaccg ccccgagctc
     9661 ggggagacgc tcgccgacgc cgggatcctc tacgcgccgg acttcatcgt gaacgccggc
     9721 gggctcatca acgtcgcgga cgagctgatg ggctacgacc gcgagcgggc gatgaagcag
     9781 gtcgaggaca tcttccgaac gctgcgcgag gtcttccgca tctcgaagaa ggaggacgtc
     9841 accccggccc gggcggcgga gctcgtcgcc gaggagcgga tccgcagcgt cagccgcctc
     9901 cgcaagctct acgtcccggg cgagcgcggc cacgggacgc tccgccgcta ggcccgctcc
     9961 cggcccctcg gctatcctgt tcgtccggac gaggagacgc gttgaccgac gacaccgtac
    10021 gcatcctgaa ccccgacggc ggggtcgacc cccaggcccg ccaggcgctc gacctcaagg
    10081 atgaggatct cctggagctg taccgctcga tggtcctcac acgcaacgtc gacctcgagt
    10141 gcaccgccct ccagcgccag ggggagctgg ccgtctaccc gccggcgatc ggccaggaag
    10201 cggcgcaggt cgggagcgcc ttcgccctcg gggaccagga catggtgtac ccgtcgtacc
    10261 gagaggtcgg cgtcgcgatc gtccgcgggc tggacatcgt cgagtacatg cacttccacc
    10321 gcggcacctg gcacgggggc gtcgacgacc cggtcgaccg gcagttcggc atgatctcgg
    10381 tcccggtcgg gagccagacc ctccacgcga ccggtcacgc gatgggcacg aagctcgacg
    10441 gcaaggacgt cgtgacgatc acctacttcg gggacggagc gacctcgcag ggcgacgtgc
    10501 acgaggcgat gaacttcgcc ggcgtcttcg gggcgccggt cgtcttcttc tgccagaaca
    10561 accagtgggc gatctcggtg ccgctggagc gccagaccgc gggcgagatc taccgcaagg
    10621 ccgagggcta cggcttcccg ggcgtccgcg tcgacgggaa cgacgtgctg gcggtctacc
    10681 gcgtgacgcg cgaggccgtc gagcgagcac gcaacgagca ccagccgacc ctgatcgaag
    10741 ccgtcaccta ccggatgggg ccccactcga cggcggacga tgccacccga taccgcgaga
    10801 aggccgacgt cgaccggtgg caggagctcg acccgatcgc gcgctaccgg acgttcctcg
    10861 aggcggaggg catcgccgat cagtcgttct tcctcgaggc cgaggccgac gggaaggagc
    10921 tggcggccac cgtccgcgcc gggatccagg gagccccgcc gcggcaggtc gaggacctct
    10981 tccggtgggt cttcgccgac ctccccccgc acctcgcgcg gcaggaggag gagatgctcc
    11041 ggctggcgaa ggaggaggag gatggctgag ctgacgatgg cccaggcgct gaaccgggcg
    11101 ctgggcgatg cgctcgccga ggacgaccgg gtgctgatcc tcggcgagga cgtcggaaag
    11161 ctcggcggcg tcttccggat caccgatggg ctccaggacc ggttcggcac atcgcgggtc
    11221 ttcgacacgc cgctcgccga gtcggcgatc gcgggggtct ccgtgggtct ctcgcaggcc
    11281 ggctggaagc ccgtgtgcga gatgcagttc gaggcgttct cctacccggc gctcgacgag
    11341 gtgatctcgc acatcgcgaa gtaccgctac cgctcgaagg gaacgacgca ggtgccgatg
    11401 gtgatgcgca tcccctacgg cggcgggatc ggcgcggccg agcaccactc cgagagcccg
    11461 gagacgtact acgcgcatac cgcgggcctg aaggtggtcg tgccgtcgac gccgctcgac
    11521 gcctacaccc tcctccggcg ggcgatcgac gaccccgacc cggtcatctt cttcgagccg
    11581 aagagccgct actggtcgaa ggaaccgggc gagccgcaga tggaggggcc cgggatcggc
    11641 gaggcgcgcg tcgctcgcga gggttcgcac gcgaccctga tcgcgtgggg cgcgatggtc
    11701 acgcgctgtc tcgaagcggc tgcggccgcc ggagaggacg gggtcgaggt cgaggtgctc
    11761 gacctccgct cgctcgtccc cctcgacctc cacgcgctcg cgacatcggt ccagaagacg
    11821 ggccgggcgg tcgtcgtgca cgaggcgccg atgaccgccg ggttcggcgc cgaggtggtc
    11881 tcgcgcatcg tcgaggattc cttcgaccac ctggaggcgc cgccgctgcg cgtgaccggg
    11941 tacgacacgc cctacccgcc ggcgacgttg gaggagcact acctcccgag cgtcgaccgg
    12001 atcctcctgg ccctcgagaa ggtcctgacg cactgatggc cgagcgcacc ttcaacctgc
    12061 ccgacctcgg tgaggggctc gaggaagccg agatcgtcga gtggaaggtc tccgcgggcg
    12121 acacggtcga gctgaaccag ccgctcgtcg acgtgaacac cgcgaaggcg ctcgtcgaga
    12181 tcccctcccc ctgggcggga acgatcgtga agacgtacgg ctcggacggc gaggtcctca
    12241 aggtcggaga accgctcgtg acgatcgacg tcggcggtga ggccgaggcc ggggcagcac
    12301 ctccgaccga cgagccgaag aagcgtgagg cggtcctcgt cggctacggc gtggaccagg
    12361 agacgaagag cgcacgacgc cggcgcctcc gccccccggg gccgcggcag gcgcccgcgg
    12421 cagcctcggc cgtgagcgcc gacacggggg ccgcatacac cgacgtcgag ccccagccgt
    12481 cgcctcccgc tgggcgggcg ctcgcagcgc cacccgtccg gaagctcgcg aaggagctgg
    12541 gcgtggacct gtcgacgatc gccgggagcg gccccaacgg gcgcgtcagg cgggaggatg
    12601 tcgaggccgc cgccggccga ggcacggcgc cgcccacgcc cctccggggg atccccatca
    12661 cgatcagcac gggctcggag acgcgcgtgc cgctgcgcgg ggtccggcgc ctcatcgccg
    12721 agaagatggc gcgatcgtgg gcggaggtcc cccacgtcac gaccttccac gtcgcggacg
    12781 cgaccaacgt cgaggccctc cgccgcgagc tcaccgagga ggccggggcg aaggtgaccg
    12841 ccctcgcggt cgtcgtccga gcgttcgccg agatctgcac caagcacccc gagctgaacg
    12901 cccgccatga ccccgaggcg aacgagctcg tgctccacgg atcgaagcac gtggggatcg
    12961 ccaccgacac cgacgagggc ctcgtcgtcc ccgtcgttcg ggatgtccag gcgaagggga
    13021 tcgtgaccgt ggcgcgggag atcgccgggc tcgtcgaggc ggcgcggacg cgtcgcgcca
    13081 cccccgacca gctgacgggc gggacggtca ccgtcacgaa cgtcggaacg ttcggctccg
    13141 acttcgggac gccgatcatc aacgtccccg aggtggcgat cctcgcgctg ggaacgatca
    13201 agcaccgccc actcgtggtg gacggatccg tcgtcgctcg gccgacggtg accttgtcgc
    13261 tctccttcga ccaccggttc atcgacggcg cccatgccga ccgggcgatg accgacctcc
    13321 gcaagcttct cgagagcccc ttcaagctcg gcgcgctccc ccgcggttga agatcgtctc
    13381 gctcctgcca tcggcgaccg agatcgtctt cgcgctcggc ctgggcgaca acctcgaggg
    13441 cgtgacctac gagtgcgacc atcccgacga ggctcggacg aagccggtcg tggtcgaggg
    13501 ggcgctcccg caggatcggc cgctgagcgc acgcgagatc gaggacgcgg tccgcgagca
    13561 catggacgcg cgcgaaccga tctacaccct gcacaaggac ctgatccaga agatccagcc
    13621 cgacctgatc ctcgcccagg acctctgccg cgtgtgcgcg gtgccttccg ggcaggtcga
    13681 ggacgcgctc gccgagctgg ggacggaggc cgaggtgctg tcgctggacc cgcacgacct
    13741 cgagggcatc ctcgcatcga tcgtgcgggt cggccgcgcg accgggacgg aggaacgcgc
    13801 cgccgaggtc gtcgaggggc tgcggcagcg gctcgaggcg ctgcgcgcga cggcggttcg
    13861 cctccccacg atccgcacgc tcgcgctcga gtggtcggac ccgccctacg tgggcggtca
    13921 ttgggtcccc gagatggtcc agctcgtcgg cggggagaac ctgctgaacg ccccgaagga
    13981 gcggtcgcgg gtcgtgacct ggcgccaggt cgccgacgca cagcccgagg tgatcgtatt
    14041 catgccctgc ggctattacc tcgaggaggc ggaggccgag ggggcacggc tcttcgagaa
    14101 ccccgagctc cgggccacga ccgcggtcgc gagcggagcc gtcttcgcgg tcgatgccac
    14161 gtcgtacttc tcccgcccgg gtccgcgcat cgtcgacggc gccgagatcc tgagctgggc
    14221 gatccatccg gaggcgttcc cggagccgga tctcgaccgg atagtccgga tcacccccta
    14281 accgtccccc atctggggta aagggcctcg ccgatcggcc gattcattta gggagaagga
    14341 ccctccagtg cccgccgggg ggacccagga tcgagatgga aggcgggctt tgaagaccca
    14401 ggcgggacga tccatcggcg tggcgctcgc gacgatcgtg gcggcctcgc tcctcggcac
    14461 gctcgccccg gcagcccagg cggcgtcacg cccgacgtgc ctcgggaagc ctgcgacgat
    14521 cgtggcgaag cggaaccaga ccgtggtgaa ggggacgccc aaggccgatg tgatcgtcgg
    14581 gtcttcgatc gggcagacga tctacggcta cggcggcaac gaccggatct gtgccggggg
    14641 cgggaacgac aaggtctacg ggggtgccgg cgccgaccga cttcacggcg ggagcgggaa
    14701 cgaccgcctc tacgg
//
LOCUS       DATLGG010000009         3804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_221966, whole genome shotgun sequence.
ACCESSION   DATLGG010000009 DATLGG010000000
VERSION     DATLGG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221966"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1088)
                     /locus_tag="VM638_00250"
     CDS             complement(39..1088)
                     /locus_tag="VM638_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVM10889.1"
                     /translation="MTAAPRDERRWPPVSYWMKATAGVLVVLALARGVIAVANVLVLV
                     VIAAVLAIGFEPAVRFLQGRGFRRGWAVASIFLAGTLVVAGFVAVVLPIIVREIADLV
                     REAPGFFRRAQHESDLVRRLEEQFDLSNRLRSLGTQLPATALALVRSVTALVFNSITV
                     AILTIYFMVAMPSLRRGIARMLDRDDREDFESILETSTQRVGGYVLGNIAVSIVAGVV
                     SFIALLVIGIPYAAALAFWVALTDLVPTIGAILGMLAAGLVAAFVGPVELVATLAFFL
                     VYQQIENYVIAPRVMRKAIDMSPAAVIVAVLIGGTLAGVVGALLALPVAAIAKVAVTE
                     LYLRDRIDAVDAAEG"
     gene            complement(1085..1942)
                     /locus_tag="VM638_00255"
     CDS             complement(1085..1942)
                     /locus_tag="VM638_00255"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HVM10890.1"
                     /translation="MRVGFVVHPARERAVEAAGKLVAWCRERGLVTRSLAEADVGADE
                     SLAPGSFVADLALVVSVGGDGTLLRAARLADLAEAPILGVNVGRLGFLTECEPDDAPE
                     VLEAFLADRHVFDERTALVAEVEGADAAEPEWALNEVVVEKRSPRRMVTLATAVGDSE
                     ITTFSADGVIVATATGSTAYSFAARGPIVSPSLACLIITPVAPHMVFDRALVLGPDEQ
                     VRLEVRGSEPGLLSADGRPERELPVGARVTIRRAERSARLVRRAGAESFYSLLRRKFS
                     LPAGSPGRS"
     gene            complement(1939..2724)
                     /locus_tag="VM638_00260"
     CDS             complement(1939..2724)
                     /locus_tag="VM638_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HVM10891.1"
                     /translation="MRRRLDQELVRRGLTRSRSEAREAIDAGRVTVAGRPALKAAGLV
                     APDEPVRLEGPARRYVSRGGEKLAAALDRFGVDPRGRRCLDAGASTGGFTDVLLQRGA
                     AHVVAIDVGYGQLAWSLRSDPRVTPIERTNIRDVGSTDLPYRADLLVADLSFIRLGKV
                     LPALAALAASDAEAVLLVKPQFEAGPGAVGRGGVVADPSVWRRVLADVLEDARAAGFG
                     PQGLMASPLRGPAGNAEFLLHARRGVVGNDVDVDAAIREVPEP"
     gene            complement(2721..3128)
                     /locus_tag="VM638_00265"
     CDS             complement(2721..3128)
                     /locus_tag="VM638_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10892.1"
                     /translation="MRRYVEAGMGVLSSRRAEELARKMVEQGQTGKDQVTKVARDLID
                     WSKKNGERLAKQVRSEVRKQIVALGVATKSDVETLRRRIDKLEGRGSAARSTGATGSS
                     PKKSTAKKSTAKKTTTAKPTAAKSTTRKTTRPS"
     gene            3221..>3804
                     /locus_tag="VM638_00270"
     CDS             3221..>3804
                     /locus_tag="VM638_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVM10893.1"
                     /translation="MRRGHRFLASTLGNLAAAGAGRGLRSARRSGVEDEEPLGTIRGN
                     VEWVSGTGGSRIYTESFPGSDPRGGTVVLTHGWCLTEAAWHHQKRDLGRGRHTVVTWD
                     LPGHGRSTGGADGASLELATDALAAVLRRHEGRPILLVGHSLGGVVTLAGLARGLSAD
                     VRGAILVSTPLMHLAASAAGSWLGAGLETRLLARA"
BASE COUNT          544 a         1465 c         1314 g          481 t
ORIGIN      
        1 gcccccggtt cccgccgggg gcccctcttt ttcccccttc agccctcggc ggcgtcgacc
       61 gcgtcgatcc ggtcccgcag gtagagctcg gtcaccgcca ccttggcgat cgccgcgacg
      121 ggcagggcca ggagcgcgcc cacgaccccc gcgagggtcc cgccgatcag gaccgcgacg
      181 atgacggccg ccggcgacat gtcgatcgcc ttacgcatca cccgcggggc gatgacgtag
      241 ttctcgatct gctggtagac gaggaagaac gcgagcgtcg ccacgagctc gacgggaccc
      301 acgaaggccg cgacgaggcc ggccgcgagc atcccgagga tcgcgccgat cgtcgggacc
      361 aggtccgtca gcgccaccca gaaggcgagc gcggccgcgt acgggatgcc gatcaccagc
      421 agagcgatga acgagacgac gccggccacg atgctgacgg cgatgttgcc gagcacgtag
      481 ccacccaccc gctgcgtcga cgtctccagg atcgactcga agtcctcccg gtcgtcgcgg
      541 tccagcatcc gcgcgatgcc gcgtcggagc gacggcatcg cgaccatgaa gtagatcgtg
      601 aggatcgcga ccgtgatcga gttgaacacc agggcggtca cgctgcggac gagggcgagc
      661 gccgtcgccg ggagctgcgt tccgagggat cgcagccggt tcgagaggtc gaactgctcc
      721 tcgaggcgcc gaacgaggtc gctctcgtgc tgggcgcgac ggaagaaccc cggcgcctcc
      781 cgcacgaggt cggcgatctc ccggacgatg atcggcagga cgacggcgac gaaccccgcc
      841 acgacgaggg tcccggccag gaagatcgag gccacggccc agccccggcg gaacccgcga
      901 ccctgcagga agcgcacggc cggctcgaag ccgatcgcca ggacggccgc gatcacgacc
      961 aggaccaata cgttcgcgac cgcgatcacg ccgcgggcga gcgcgagcac tacgaggacg
     1021 cccgccgtcg ccttcatcca gtacgagacc ggcggccagc gacgctcgtc ccgcggggcc
     1081 gccgtcacga ccgccccggc gacccggccg gaagggagaa cttccggcgc agcagcgagt
     1141 agaacgactc cgcccccgcc cggcggacga ggcgcgcgga tcgctccgcc cggcggatcg
     1201 tgacgcgcgc cccgaccggg agctcccgtt cgggacggcc atccgccgac aggaggccgg
     1261 gctcgctccc tcggacctcc agccgcacct gctcgtcagg gccgaggacg agggcgcggt
     1321 cgaagaccat gtgcggagcc accggcgtga tgatgaggca ggcgaggctc ggcgagacga
     1381 tcggcccgcg ggccgcgaac gagtacgcgg tcgacccggt cgccgtcgcc acgatcacgc
     1441 cgtcggcgga gaacgtcgtg atctccgagt cgccgaccgc cgtcgcgagc gtgaccatcc
     1501 tccgcgggga gcgcttctcc acgaccacct cgttcagggc ccactcgggc tcggccgcgt
     1561 ccgctccctc gacctcagcc accagcgcgg tccgctcgtc gaagacgtga cggtcggcga
     1621 ggaacgcctc gaggacctcg ggggcgtcgt ccggctcgca ttcggtcagg aacccgaggc
     1681 ggccgacgtt cacgccgagg atcggggcct ccgcgaggtc ggcgaggcgc gcggcgcgca
     1741 ggagcgtccc gtcaccgccg accgacacga cgagggcaag gtcggcgacg aaggatcccg
     1801 gcgccaggga ctcgtccgcg cccacgtccg cctccgcgag cgagcgggtg acgaggccgc
     1861 gctcgcggca ccaggcgacg agcttccccg ccgcctcgac cgcccgctct cgcgcgggat
     1921 gcacgacgaa gcccaccctc acggctcggg cacctcccgg atcgcagcgt cgacgtccac
     1981 gtcgttcccg accacccctc gcctcgcgtg caggaggaac tcggcgttgc cggccgggcc
     2041 ccgcagcggc gatgccatga ggccctgggg accgaacccg gccgcccgcg cgtcctccaa
     2101 gacgtcggcg agcacgcggc gccacacgct gggatccgcc acgacccccc cgcgacccac
     2161 ggccccgggc ccggcctcga actgtggctt cacgagcagc accgcctcgg cgtcgctcgc
     2221 ggcgagcgcg gcgagcgcgg gaagcacctt ccccagccgg atgaacgaga gatccgccac
     2281 cagcaggtcg gcccggtagg gcagatccgt cgagccgaca tcgcggatgt tcgtgcgctc
     2341 gatcggcgtc acgcgcggat cgctgcgcag ggaccacgcc agctgcccgt acccgacgtc
     2401 gatcgccacg acgtgcgcgg cgccgcgctg cagcagcacg tcggtgaacc cgcccgtcga
     2461 tgccccggcg tcgaggcagc gtcggccgcg cgggtcgacg ccgaaccggt cgagcgccgc
     2521 ggcgagcttc tcacccccgc gcgagacgta gcggcgggcg gggccttcca gcctcaccgg
     2581 ctcgtcgggc gccaccaggc ccgcggcctt gagcgccggc cgcccggcga ccgtcacccg
     2641 tccggcgtcg atcgcctcgc gtgcctcgga tcggctccgc gtgaggccgc ggcggacgag
     2701 ctcctggtcg agccggcgcc tcaactcggt ctcgtggtct tcctcgtggt ggacttcgcg
     2761 gccgtcggct tcgccgtggt cgtcttcttc gccgtgctct tcttggccgt cgacttcttc
     2821 ggggacgatc cggtggcgcc cgtggagcgc gccgccgacc cccggccctc gagcttgtcg
     2881 atgcggcgcc ggagcgtctc gacgtcgctc ttcgtggcca ccccgagcgc gacgatctgc
     2941 ttccggacct cgctccgaac ctgcttcgcg aggcgctccc cgttcttctt cgaccagtcg
     3001 atcagatcgc gagcgacctt ggtcacctgg tccttgcccg tctgtccctg ctcgaccatc
     3061 ttccgcgcca actcctccgc gcgccgcgag gagagcacgc ccatccccgc ctccacgtac
     3121 ctgcgcatcg cgtccagcat cccgcctccc tggtccgacg tcggcagaag gataacaacg
     3181 ggcggtcgcg ctatgatcgc gctcgatcgg cacccttccg atgagacgag gacatcgctt
     3241 cctggcgtcg acgctcggca acctcgccgc cgccggcgcg ggacggggcc tccgaagcgc
     3301 gcgacggtcg ggcgtcgagg acgaggagcc gctcggcacg atccgcggca acgtcgagtg
     3361 ggtctcgggg accggcggtt ctcggatcta taccgagtcc ttcccaggct ccgacccccg
     3421 gggcggcacg gtcgtcctca cccacggatg gtgcctgacc gaagccgcct ggcaccacca
     3481 gaagcgcgac ctcggccgag ggcgtcacac ggtcgtgacg tgggacctcc ccgggcacgg
     3541 ccgctcgacc gggggtgccg acggcgcctc cctcgagctg gcgaccgacg ccctggcggc
     3601 ggtcctgcga cggcacgagg gccggccgat cctcctcgtc gggcacagcc tcggcggcgt
     3661 cgtcaccctc gcggggctcg cccgcggtct ctcagccgac gtccgcggag cgatcctcgt
     3721 ctcgacgccg ctgatgcacc tcgcggcgtc cgcagcgggt tcgtggctgg gggcgggcct
     3781 cgagacgagg ctcctcgctc gggc
//
LOCUS       DATLGG010000010         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_47999, whole genome shotgun sequence.
ACCESSION   DATLGG010000010 DATLGG010000000
VERSION     DATLGG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47999"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VM638_00275"
     CDS             <1..552
                     /locus_tag="VM638_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HVM10894.1"
                     /translation="AVTLPEPEEVDADRFMPSSRSIAFRREVFEKAERYPEWLDIGED
                     MYLNHRLRELPIRMDFAPEAIAYWRPRPNVSETWRQYFRYAEGDAVAGMYPERHAVRF
                     ATYGLLAWALARRNRALLALLGAGATTLVGRRARRLFGLLHDPSDRARAMLSLPGHIA
                     LADLAKMAGYLSGLSRRSARERR"
     gene            549..1661
                     /locus_tag="VM638_00280"
     CDS             549..1661
                     /locus_tag="VM638_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVM10895.1"
                     /translation="MTTSLDDFGKLFPALERYTWLNTATVPPGCEPVLRAAREALEGW
                     ERGEFSWQAWEGEAYATRELFARLIGVEARTVALMSSVAEAASTVAAALPPGKVVVGE
                     REFHSNIFPWTALRERGFEVEEVQAEDDVVSTDALIAAVDERTVLVAVSEVQSSNGYR
                     VDVRRLAEACRAAGARLFVNLTQSLGALRFDAAEVGADYVAAHGYKWLLGMRGAAWMH
                     VREDRLEALRPIAPNWHSIRDPYADYYGSEFDPAPGAGWLDTSIDWMPWIGSRAALEL
                     VASLDREQVERHALALSAAFRAEVTARGFEVVREEVPTQIVSLRVPDPEALVERLRER
                     RVVGAVRGGSLRLGFHGFNRGEDVETALDALEASAR"
     gene            complement(1658..1933)
                     /locus_tag="VM638_00285"
     CDS             complement(1658..1933)
                     /locus_tag="VM638_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10896.1"
                     /translation="MAHPAPLKKMQEQTRVERASQRRQAPGPRCVQSGRIQVTDICSE
                     VKTLSEMLLICERRYANALSAGNDEKAGRWAAAGEALLVALRPVKRD"
     gene            complement(2043..>2274)
                     /locus_tag="VM638_00290"
     CDS             complement(2043..>2274)
                     /locus_tag="VM638_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVM10897.1"
                     /translation="HKANVWIELSGWAPRYLPREVVREAKKRLNERTLFGSDYPFIPL
                     ERWFGEFDQLDIDGDAAENIRLRNAQRLLGLG"
BASE COUNT          297 a          803 c          816 g          358 t
ORIGIN      
        1 gcagtgacgc tgcccgagcc cgaggaggtg gacgccgacc gcttcatgcc gtcctcgcgg
       61 tcgatcgcgt tccggcggga ggtcttcgag aaggccgagc gctacccgga gtggctggac
      121 atcggcgagg acatgtacct gaaccaccgc ctgcgtgagc ttccgatccg gatggatttc
      181 gcccccgagg cgatcgcgta ctggcggccc cggccgaacg tgagcgagac ctggcggcag
      241 tacttccgct acgccgaggg cgacgcggtg gccggcatgt accccgagcg gcacgcggtg
      301 cggttcgcca cctacgggct cctcgcgtgg gcgctcgcac ggcggaaccg agccctcctc
      361 gcgctcctcg gagccggcgc cacgacgctc gtcgggcgac gggcccgccg gctcttcggg
      421 ctgcttcacg atccctccga tcgcgcacgg gcgatgctct cgctccccgg ccacatcgcg
      481 ctggccgacc tcgcgaagat ggcgggctat ctcagcggcc tttcccgccg atctgcgcgc
      541 gagcgccgat gaccacctcg ctcgacgact tcggcaagct cttccccgcg ctcgagcgct
      601 acacgtggct caacaccgcg accgtcccgc cgggctgcga gccggtcctg cgcgccgcgc
      661 gtgaggcgct cgaggggtgg gaacgcgggg agttctcttg gcaggcctgg gagggcgagg
      721 cctatgcgac ccgtgagctg ttcgcacgct tgatcggcgt cgaggcacgc acggtcgcgc
      781 tgatgtcgtc ggtcgccgag gcggcctcga cggtcgccgc cgcgctgccg cccgggaagg
      841 tcgtcgtcgg cgagcgcgag ttccactcga acatcttccc gtggacggcg ctccgcgagc
      901 ggggtttcga ggtcgaggag gtccaggcag aggacgacgt cgtctcgacg gacgcgctga
      961 tcgcggcggt cgacgagcgg acggtcctgg tcgccgtcag cgaggtgcag tcctcgaacg
     1021 gctaccgcgt ggacgtgcga aggctcgcgg aggcgtgccg cgcggccggg gcgcggctct
     1081 tcgtgaacct cacccaatcc ctcggggcgt tgcgcttcga cgcggccgag gtcggcgccg
     1141 actacgtcgc cgcacacggc tacaagtggc tcctcgggat gcgcggcgcc gcctggatgc
     1201 atgtgcggga ggaccgtctc gaggcccttc gcccgatcgc tccgaactgg cacagcatcc
     1261 gcgaccccta cgccgattac tacggcagtg agttcgaccc cgcaccgggc gctgggtggc
     1321 tcgacacgtc gatcgactgg atgccgtgga tcggatcacg cgccgcgctc gagctcgtcg
     1381 cctccctcga ccgcgagcag gtggagcggc acgccctggc gctgtctgcg gcgttccgcg
     1441 cggaggtgac ggcgaggggc ttcgaggtcg tgcgcgagga ggtcccgacc cagatcgtga
     1501 gcctgcgggt gccggatcct gaagcgctcg tcgaacgcct gcgcgaacgg agggtcgtgg
     1561 gggcggtccg cggcggatcg ctccgcctcg ggttccacgg gttcaaccgc ggcgaggacg
     1621 tggagaccgc cctcgacgcc ttggaggcgt cggcgcgtta gtcgcgcttg acggggcgga
     1681 gcgccaccag cagcgcctcg ccggcggccg cccagcggcc ggccttctcg tcgttgcccg
     1741 ccgacagcgc gttggcgtaa cgccgctcgc agatcaagag catctccgag agcgtcttca
     1801 cctccgagca gatgtcggtg acctggatcc gcccggactg gacgcaacgg ggcccgggcg
     1861 cctgcctccg ctggctcgct cgctcgactc tcgtctgctc ctgcatcttc ttcaatggcg
     1921 ccgggtgcgc catcctctct gaccagggat ttcgccaccg cccccgcgat gtcctgcccg
     1981 gcaagcggaa tctcttcccg cggcggaccc ggatgccggg aaccgcccga tttttcccgg
     2041 gctcagccga ggccgaggag gcgctgggcg ttgcgcaggc ggatgttttc ggcggcgtcg
     2101 ccgtcgatgt cgagctggtc gaactccccg aaccagcgtt cgaggggaat gaaggggtag
     2161 tcgctgccga acagcgtccg ctcgttcagg cgcttcttcg cctcccggac gacctcgcgc
     2221 gggaggtagc ggggcgccca gccggagagc tcgatccaca cgttggcctt gtgg
//
LOCUS       DATLGG010000011         5266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_16450, whole genome shotgun sequence.
ACCESSION   DATLGG010000011 DATLGG010000000
VERSION     DATLGG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16450"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VM638_00295"
     CDS             complement(<1..128)
                     /locus_tag="VM638_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HVM10898.1"
                     /translation="MSTMPFYVAPEQIMKDRSEYARKGISRGKPVVALEYADGILF"
     gene            complement(125..898)
                     /gene="prcB"
                     /locus_tag="VM638_00300"
     CDS             complement(125..898)
                     /gene="prcB"
                     /locus_tag="VM638_00300"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HVM10899.1"
                     /translation="MPIGANPSFFDLIRDFDRQPDPVRMPGDAPPPGAPHGTTVVALR
                     FRDGVVMAGDRRAVEGYTIADNEMQKVFPADDHSAVGIAGVAGQAIEMVRLFQTELEH
                     YEKVEGEELSLEGKANRLAQMLRGMFPMVMQGLVVVPIYGGFDVRRTEGRIFRYDVIG
                     GRYEESDYAATGSGGMHAKASMKKRWRRGLDRAGALEVAVEALVDAGESDTATAGPDL
                     IRGIFPTVHVITAEGVEEVPDEEVRGAAERILRTREESR"
     gene            complement(910..1110)
                     /locus_tag="VM638_00305"
     CDS             complement(910..1110)
                     /locus_tag="VM638_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HVM10900.1"
                     /translation="MPEQEQKRIQKKDGGEGAGDEAGQSTATTKGEKLKEEMDDILDE
                     IDEVLEENAEEFIKSYVQKGGE"
     gene            complement(1173..2657)
                     /gene="dop"
                     /locus_tag="VM638_00310"
     CDS             complement(1173..2657)
                     /gene="dop"
                     /locus_tag="VM638_00310"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HVM10901.1"
                     /translation="MAIPKVMGIETEYGISAPGSSEFNPVLASSIVINSYAGALRRIR
                     WDYEQESPLRDARGFEPVQDREPGDEDLGLANVILPNGARYYVDHAHPEYSTPECADP
                     RDLVVHDKAGERILERSIELAQETLPPGERIAIYKNNTDGKGNSYGTHENYLVDRATP
                     FARIVRDLMPFFVTRQVFTGAGKLGIEGSRVNGDAPIYQLSQRADFFEAEVGLETTLK
                     RPIINTRDEPHADPEKYRRLHVIIGDANMAEVATFLKVGSTAIILKMIEDDLLPDLSL
                     ENPVNALHRISRDPSLRATVRLADGRTVTALELQWEYLDHAKAYVKENDDTPRNSEVL
                     ARWESVLTALEDDPSTLAGQLDWVAKRRLLEAYRERDGLAWDDPKLALVALQYHDVRR
                     DRGLYYKLVDSGKIERMVAESEVQEAVSHPPEGTRAYFRGTCIRRFGENITAASWDSI
                     IFDTGQDALQRVPMREPLRGTREMVGELLETVEGATELVEAIRS"
     gene            complement(2694..4445)
                     /gene="arc"
                     /locus_tag="VM638_00315"
     CDS             complement(2694..4445)
                     /gene="arc"
                     /locus_tag="VM638_00315"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HVM10902.1"
                     /translation="MQEDPQRRDEERDKELHELQSQVKFLEEELALVRRRLHSSPRQV
                     KVLEERLLEAKSEVARALAQNEKLAAALKTERERIEALREEVEKLTQPPASFGVFLGT
                     NDDDTVDVFTSGRKMRVNVAPDVNRDDLVRGAEVILNEGLNVIEVLPQDRIGEVVTVK
                     EILGDDRVIVLGRGDDEMVALISRALRGTPFRVGDALLYDSRSGHVLERLPKEEIEEL
                     VLEEIPDVSYQDIGGLAGQIEDVRDAVEMPFLYSELFHEHQLRPPKGVLLYGPPGCGK
                     TLIAKAVANSLAQQVAQKTGRSGVHSYFLNVKGPELLNKYVGETERQIRVIFQRAKEK
                     AQEGFPVIVFFDEMDSIFRIRGSGISSDVENTIVPQLLSEIDGVETLKNVIVIGASNR
                     EDLIDPALLRPGRLDVKIKIERPDADSARDIMSKYLTPQIPIHPDDVKAANGDAAAAV
                     KRIIDRTIEQMYSDGDHNRFLEVTYQNGDKEVLYFKDFNSGAMIENIVARAKKMAIKR
                     FLQSGEKGVTMEDMLQAVRLEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLLGDR
                     EDGAGRQVEQVRAGQYL"
     gene            complement(4488..>5266)
                     /locus_tag="VM638_00320"
     CDS             complement(4488..>5266)
                     /locus_tag="VM638_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HVM10903.1"
                     /translation="DHRPLAAGDRIVLVDSRGRRYLLNLADGGVFHWHGGTIAHDEVI
                     GQPEGTRVRAPSGAALTAFRPRLADFVLKMGRGAQVIYPKDLGPMLIYGDVYPGASVL
                     EAGTGSGALTMALCRAVGAEGRVVSCELRPGFADVARRNIGSFFGSIPPQLELRTADV
                     RELDGERFDRMLLDLPDPWAALPAATRVLEPGGVFCAYLPTTAQVQSLVLNLEKAGFG
                     EVETFEVLHRSWHVSARSVRPDHRMVGHTGFLTTARRTSA"
BASE COUNT          731 a         1852 c         1763 g          920 t
ORIGIN      
        1 atgaagagga tcccgtcggc gtactcgagc gcgacgacgg gcttgccacg cgagatgccc
       61 ttgcgggcgt actccgagcg gtccttcatg atctgctcgg gcgcgacgta gaacggcatc
      121 gtgctcatcg gctctcctcc cgggtgcgga ggatccgctc ggcggctccg cggacctcct
      181 cgtccggaac ctcctcgacc ccctcggcgg tgatcacgtg gaccgtcggg aagatcccgc
      241 ggatgaggtc cggtccggcc gtcgccgtat cggactcgcc ggcgtccacc agcgcctcga
      301 cggcaacctc gagcgccccg gcccggtcga ggccgcgacg ccaccgcttc ttcatgctgg
      361 ccttcgcgtg catgccaccg gacccggtcg ccgcgtagtc ggactcctcg tagcggccgc
      421 cgatcacgtc gtagcggaag atgcggccct cggtgcggcg aacgtcgaaa cccccgtaga
      481 tcgggacgac gacgagaccc tgcatgacca tcgggaacat cccgcgcagc atctgcgcga
      541 ggcggttcgc cttgccctcg agggagagct cttcgccctc gaccttctcg tagtgctcga
      601 gctcggtctg gaagagccgc accatctcga tcgcctggcc ggcgaccccg gcgatcccga
      661 cggccgagtg gtcgtcggcg gggaagacct tctgcatctc gttgtcggcg atcgtgtagc
      721 cctcgaccgc acgccggtcc cccgccatca cgacgccgtc gcggaagcgg agggcgacga
      781 ccgtggttcc gtgcggggcc cctggcgggg gcgcgtcacc cggcatgcgg accggatccg
      841 gctgacggtc gaagtcgcgg atcagatcga agaagctcgg gttcgcgccg atcggcattc
      901 cgtcgccgat cattcgccgc ccttctggac gtagctcttg atgaactcct cggcgttctc
      961 ctcgaggacc tcgtcgatct cgtcgaggat gtcgtccatc tcctccttga gcttctcgcc
     1021 cttcgtggtc gccgtggact ggccggcctc atcgccggcg ccctctccgc cgtccttctt
     1081 ctggatccgc ttctgctcct gctctggcaa cgttgctcct tacgctcgtg gacctggcct
     1141 attgtaggcc cgcctccggg cggggtgccg ggtcaggatc ggatcgcctc gacgagctcc
     1201 gtcgcgccct cgaccgtctc gaggagctcg cccaccatct cccgtgtccc acgcaggggc
     1261 tcccgcatcg ggacccgctg gagggcgtcc tgacccgtgt cgaagatgat cgagtcccac
     1321 gaggccgccg tgatgttctc gccgaagcgc cggatgcacg tgccccggaa gtaggcgcgc
     1381 gtcccctccg gcggatggct cacggcttcc tgcacctccg actccgcgac catccgctcg
     1441 atcttccccg agtcgacgag cttgtagtag aggccgcggt cccgacggac gtcgtggtac
     1501 tggagcgcga cgagcgcgag cttcgggtcg tcccatgcga gcccgtcacg ctcccggtac
     1561 gcctccagca gtcggcgctt cgccacccag tcgagctgac cggcgagcgt cgagggatcg
     1621 tcctcgagcg ccgtcagcac ggattcccac cgcgcgagca cctcgctgtt ccggggtgtg
     1681 tcgtcgttct ccttcacgta cgccttcgcg tgatcgaggt actcccactg cagctcgagg
     1741 gcggtgacgg tccggccgtc ggcgagccgc acggtcgcac gcaggctcgg atcccgggag
     1801 atgcggtgga gcgcgttcac ggggttctcg agcgagagat cgggaaggag gtcgtcctcg
     1861 atcatcttga ggatgatcgc ggtggagccg accttcagga aggtcgcgac ctcggccatg
     1921 ttcgcatcgc cgatgatcac gtggagccgg cggtacttct ccggatcggc gtgcggctcg
     1981 tctcgcgtgt tgatgatcgg gcgcttcagc gtggtctcga gcccgacctc agcctcgaag
     2041 aagtccgccc gctggctcag ctggtagatc ggtgcgtcgc cgttgacgcg gctgccctcg
     2101 atgccgagct tcccggcgcc ggtgaacacc tgccgcgtca cgaagaacgg catcaggtcc
     2161 cggacgatcc gcgcgaacgg cgtcgcacgg tccacgaggt agttctcgtg cgtcccgtag
     2221 gagttgccct tgccgtcggt gttgttcttg tagatcgcga tccgctcgcc cggcgggagc
     2281 gtctcctgcg cgagctcgat cgaccgctcg aggatgcgct cccccgcctt gtcgtggacg
     2341 acgaggtccc gggggtcggc gcactccggc gtggagtact cggggtgggc gtggtcgacg
     2401 tagtagcgcg cgccgttcgg caggatcacg ttcgccaggc cgaggtcctc gtccccgggc
     2461 tcgcggtcct ggaccggctc gaacccgcgc gcgtcccgca gcgggctctc ctgctcgtag
     2521 tcccagcgga tccgccggag cgcgcccgcg tagctgttga tcacgatcga cgaggcgagc
     2581 accgggttga actcggagct cccgggagcc gagatgccgt actcggtctc gatgcccatg
     2641 accttgggga tcgccatgga cctaggatac ccgcccgtcc ggacgcgacc gcgctacagg
     2701 tactggccgg cgcggacctg ctcgacctgg cgccccgcgc cgtcctcgcg atcgcccagg
     2761 agcgtgcgga cgtacacgat ccgctcgccc ttcttgccgg agatccgagc ccagtcgtcg
     2821 gggttcgtcg tgttcggcag gtcctcgttc tccttgaact ccaggcggac ggcctgaagc
     2881 atgtcctcca tcgtcacgcc cttctcgccg ctctggagga accgcttgat ggccatcttc
     2941 ttcgcccggg cgacgatgtt ctcgatcatc gccccggagt tgaagtcctt gaagtacagg
     3001 acctccttgt caccgttctg gtaggtgacc tcgaggaagc ggttgtgatc cccgtcggag
     3061 tacatctgct cgatcgtgcg gtcgatgatc cgcttcacgg cggccgccgc gtccccgttc
     3121 gccgccttga cgtcgtcggg gtggatcggg atctgcgggg tcaggtactt cgacatgatg
     3181 tcccgcgccg agtccgcgtc gggacgctcg atcttgatct tcacgtccag gcgccccgga
     3241 cggagcagcg ccgggtcgat caggtcctca cggttcgagg cgccgatcac gatgacgttc
     3301 ttcagcgtct ccacgccgtc gatctccgag agcagctgcg ggacgatcgt gttctcgacg
     3361 tcggatgaga tgcccgagcc acggatccgg aagatcgagt ccatctcgtc gaagaacacg
     3421 atgacgggga acccctcctg cgccttctcc ttcgcgcgct ggaagatcac gcggatctgc
     3481 cgttcagtct caccgacgta cttgttgagc agctcgggcc ccttcacgtt gaggaagtac
     3541 gagtggacgc ccgagcggcc ggtcttctgc gcgacctgct gagcgagcga gttggcgacg
     3601 gccttcgcga tcagggtctt cccgcagccg ggcggcccgt agagcagcac gcccttcggg
     3661 gggcgcagct ggtgctcgtg gaagagctcc gagtagagga acggcatctc gacggcgtcg
     3721 cgcacgtcct cgatctgtcc cgcgaggccg ccgatgtcct ggtagctgac gtcggggatc
     3781 tcctcgagga cgagctcctc gatctcctcc ttcgggaggc gctcgagcac gtggcccgag
     3841 cgcgagtcgt acaggagcgc gtcgccgacc cggaaggggg tcccgcggag ggcacgggag
     3901 atcagcgcca ccatctcgtc gtcaccgcgg ccgaggacga tcacgcggtc gtcgccgagg
     3961 atctccttga ccgtcacgac ctcgccgatc cggtcctggg gaaggacctc gatcacgttc
     4021 aggccctcgt tgaggatgac ctccgccccg cgcaccaggt cgtcgcggtt cacgtccggc
     4081 gcgacgttga cccgcatctt gcgtccggag gtgaacacgt cgacggtgtc gtcgtcgttc
     4141 gtgccgagga agacgccgaa cgacgcgggc ggctgcgtga gcttctcgac ctcctcgcgg
     4201 agcgcctcga tccgctcgcg ctcggtcttc agggcggctg cgagcttctc gttctgcgcg
     4261 agggcgcgcg cgacctcgct cttcgcctcg agcagtcgct cctcgagcac cttcacctga
     4321 cgcggcgagc tgtgcaagcg acgccggacg agcgcgagct cctcctcgag gaacttcacc
     4381 tggctctgga gctcgtggag ctccttgtcc cgctcctcgt ccctgcgctg cggatcttcc
     4441 tgcatcccga cctcccactc tcgcgcgtcc gagtataccc gcgcccccta cgcggacgtg
     4501 cggcgggcgg tcgtcaggaa cccggtgtgc cccaccatgc ggtggtcggg ccgaacgctg
     4561 cgcgcggaga cgtgccacga ccggtggagg acctcgaagg tttcgacctc gccgaagccc
     4621 gccttctcga ggttgaggac gagcgactgc acctgcgcgg tcgtcggcag gtaggcgcag
     4681 aagacgccgc ccggctcgag cacccgggtc gcggcgggga gcgccgccca tggatccggc
     4741 aggtcgagga gcatccggtc gaaccgttcg ccgtcgagct cgcgcacgtc cgccgtccgc
     4801 agctcgagct gcggcgggat ggagccgaag aacgagccga tgttgcggcg ggccacatcg
     4861 gcgaagcccg gccgcagctc gcaggagacg acgcggccct cggcgccgac cgcccggcag
     4921 agcgccatcg tcagggcgcc ggatcccgtc cccgcctcga ggacgctggc gccgggatag
     4981 acgtcgccgt agatcagcat cggtccgaga tccttcgggt agatcacctg cgcgccgcgt
     5041 cccatcttca gcacgaagtc ggcgaggcgc ggccggaacg cggtcagcgc cgcgccggag
     5101 ggcgcccgga cgcgggtgcc ttcgggctgc ccgatcacct cgtcgtgcgc gatcgtgccc
     5161 ccgtgccagt ggaacacgcc gccgtccgcg aggttgagga ggtaccgacg gccacgcgag
     5221 tcgaccagga cgatccgatc accggccgcg agcggccggt gatcgg
//
LOCUS       DATLGG010000012         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_190252, whole genome shotgun sequence.
ACCESSION   DATLGG010000012 DATLGG010000000
VERSION     DATLGG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190252"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /gene="steA"
                     /locus_tag="VM638_00325"
     CDS             complement(<1..801)
                     /gene="steA"
                     /locus_tag="VM638_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HVM10904.1"
                     /translation="MGLRLAGPRERALAGAACRVDARTKRLLTRIRPGEVAVIDHEDL
                     DRVAAEGLVARGASAVVNASPSITGRYPNLGPEILLEAGIPLVDAVGPLLLRKVREGT
                     ILRIEGDRVYAGDELVGVGVRQDAASVRQAMVEAEARLSGAMDAFARNTLEYMERERD
                     LLFGGVGVPEIGHDLNGRHVLVVVRGPRYREDLAALRAYIRDVKPVLVGVDGGADALL
                     EEGYRPDIVIGDMDSVSERALRAAREVIVHAYRDGRAPGRGRPDTRGRS"
     gene            complement(851..2488)
                     /gene="recN"
                     /locus_tag="VM638_00330"
     CDS             complement(851..2488)
                     /gene="recN"
                     /locus_tag="VM638_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HVM10905.1"
                     /translation="MLRELHLSGLGVIDDATLELAPGLNVLTGETGAGKTMVTVGLAL
                     ALGQRARAHLVAEGRARASAQARFDVAGPAFDEWAEDGDLVLSRVIAADGRSTGRAGG
                     QLAPLSTLAELGANLVEVHGQHQAQRLLSPATHLDLLDRFAGRDHLAAVGAYRRAFTD
                     LRQARARLEELDERGREREREKDLLAYQVREIEGVGLSPGEIGALLAEEGRLANVERI
                     REGLADARGLLDGGAADGLAAAARSIAEAADLDEEARPLAERIASLAEEARDAAGEVR
                     DRLERAELDPARLEAVRERLAAIRQLERKYGEGEAAIFAFLGEARSRLAALEGAGEER
                     DALVVEVRRRSEEVEASAAGLAGTRREAAGRLAAALEAEVHELGMAGARLVVAVRPLE
                     APGPDGSESVELLLGGGPGQEPRPLASAASGGELSRTMLACRTVLADLDEVPTLVFDE
                     VDAGIGGKAGVAVGRRLARLARSRQVLVVTHLPQIACFADRHFRVTKDAGRTVVEELG
                     EAERVEELSRMLSGMPTSEAAAHAGQLLAEAGREKAG"
BASE COUNT          330 a         1035 c          877 g          316 t
ORIGIN      
        1 tgatcgtcca cgcgtatcgg gacggccgcg ccccggggcg cggccgtccc gatacgcgtg
       61 gacgatcacc tcgcgcgcgg cgcgcagcgc acgctcggag acgctgtcca tgtccccgat
      121 cacgatgtcc ggccggtacc cctcctcgag gagcgcgtcc gcgccgccgt ccacaccgac
      181 gaggacgggc ttcacgtcgc ggatgtacgc gcggagggcg gcgagatcct cacggtagcg
      241 ggggccccgg acgacgacga gcacgtgccg cccgttcagg tcgtggccga tctcgggaac
      301 gccgacaccg ccgaagagca ggtcccgctc ccgctccatg tactcgaggg tgttgcgcgc
      361 gaacgcatcc atcgcgccgg atagccgcgc ttccgcctcg accatcgcct gccggacgga
      421 cgcggcatcc tgccgcaccc ctaccccgac cagctcgtcc ccggcgtaga cccggtcccc
      481 ttcgatccgc aggatcgtcc cctcacggac cttgcgcagc aggagcggcc cgaccgcgtc
      541 gacgaggggg atccccgcct ccagcaggat ctccgggccg aggttcggat agcgacccgt
      601 gatcgagggg ctcgcgttca ccacggcgga ggcaccacgt gccaccaacc cctcggccgc
      661 gacccggtcg aggtcctcgt gatcgatgac ggcgacctca cccggccgga tccgggtcag
      721 caggcgcttc gtgcgcgcgt cgacccggca cgcagcgccc gcgagcgcgc gctccctcgg
      781 ccccgcgagt cgcaacccca tcggtgcacc tcccggcggc gccgtcggcc gccggggaag
      841 acgatatacc tcagccggct ttctctcgcc ccgcttcggc gagcagctgg ccggcgtgcg
      901 cggcggcctc gctcgtgggc atccctgaga gcatccgcga cagctcctcc acccgctcgg
      961 cctcgcccag ctcctccacg accgtccggc cggcgtcctt cgtcacccgg aagtgccggt
     1021 cggcgaagca ggcgatctgg ggaaggtgcg tgacgacgag cacctggcgc gagcgcgcga
     1081 gccgcgccag acggcggccg acggcgacgc ccgctttgcc gccgatcccg gcatccacct
     1141 cgtcgaagac gagcgtcggg acctcgtcga ggtcggcgag gacggtccga caggcgagca
     1201 tcgtccggga gagctcgccg cccgacgccg ccgaggcgag ggggcgtggc tcctgccccg
     1261 ggccgccgcc gagcaggagc tccaccgact cgctcccgtc gggccccggc gcctccagcg
     1321 gccgcacggc gacgacgagg cgggcacccg ccatcccgag ctcgtgcacc tcggcctcga
     1381 gcgccgccgc gagccgcccc gccgcctccc gacgcgtccc ggccagcccc gcagcggacg
     1441 cctcgacctc ttcggagcgg cgtcggacct ccacgacgag cgcgtcgcgc tcctcccccg
     1501 ctccctcgag cgcggcgagg cgggatcgtg cctcgccgag gaaggcgaag atcgccgcct
     1561 cgccctcccc gtacttccgc tcgagctgtc tgatcgccgc gaggcgctca cggaccgcct
     1621 cgagacgcgc cggatcgagc tccgcccgct ccagccggtc gcgtacctcc cccgccgcgt
     1681 cccgcgcctc ctccgcgagc gacgcgatcc gctccgccag cggccgcgcc tcctcatcga
     1741 ggtcggcggc ctccgcgatc gagcgcgccg cggcggccag gccgtccgcc gcccccccgt
     1801 ccaggaggcc gcgagcgtcg gcgaggccct cccggatccg ctcgacgttc gccagccggc
     1861 cttcctcggc gaggagggca ccgatctcgc cgggagacag gccgaccccc tcgatctccc
     1921 gcacctggta ggccagcagg tccttctcgc gctcgcgctc gcgcccgcgc tcgtccagct
     1981 cctcgagacg tgcccgcgcc tgtcgcaggt cggtgaacgc gcgacggtag gcgccgacgg
     2041 cggcgaggtg gtcgcgcccc gcgaagcggt cgagcaggtc gagatgggtt gccggcgaca
     2101 ggagccgctg ggcctggtgc tgcccgtgga cctcgacgag gttcgccccc agctccgcca
     2161 gggtcgagag cggcgcgagc tgtccccccg cgcggcccgt cgagcggcca tcggccgcga
     2221 tcacacgcga caggacgaga tctccgtcct ccgcccactc gtcgaaggcg gggccggcca
     2281 cgtcgaaccg cgcctgggcg gaggcgcgcg cccgcccctc cgccacgaga tgggcgcgcg
     2341 cgcgctggcc gagggcgagg gcgagcccga cggtgaccat cgtcttgccg gctccggtct
     2401 cgccggtgag cacgttgagc cccggcgcga gctcgagcgt ggcgtcgtcg atgaccccca
     2461 gcccggagag atggagctct cgaagcatcg gcggcatcgt accgcccggc accgacacgt
     2521 cggccccgga aaactttctg catggatttg tttagggg
//
LOCUS       DATLGG010000013         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_32452, whole genome shotgun sequence.
ACCESSION   DATLGG010000013 DATLGG010000000
VERSION     DATLGG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32452"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1109)
                     /locus_tag="VM638_00335"
     CDS             complement(27..1109)
                     /locus_tag="VM638_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVM10906.1"
                     /translation="MRRSLAALLATALLTSCAALEDLGLPVTGPTFSPAPPAPAGSDT
                     IVRVVEAVRPAVVNVTTNVVTPGALTGEAQGGTGTGFIVDESGIIVTNFHVVEGGLTI
                     RVITDDGRRFDARVIGGDPNADLAVLQVEADDLPTVDLGDSDHVRLGETVVALGFALA
                     LEGGPSVTSGIISAKGRTITASDPQVGERTYEDLLQTDAAINPGNSGGPLVDLSGRVV
                     GINTAGVQAGAAENISFAIAINRAKEVIRDAIRDPEAEVAYIGVSTRTVDSSLSFQLN
                     LPVDQGALVVDVAPGGPADRAGIRPGDIIIGIGGRPVTDDEAVRSAIVEHEPGERIQV
                     SIVRRDGSRETMTVALGVRPLPIPTG"
     gene            complement(1145..1924)
                     /gene="fabI"
                     /locus_tag="VM638_00340"
     CDS             complement(1145..1924)
                     /gene="fabI"
                     /locus_tag="VM638_00340"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HVM10907.1"
                     /translation="MLLEGKRLLITGVLTPQSIAFAVARQAQEAGADVVLTGFGRGLS
                     ITQKSARRLPEPCDVLELDVNRPEQVEEVAAALGERWDALDGVLHAIAFAPEDALGGN
                     FLTAPWESVATAVRTSAYSLKELVVPLLPLLSVRGGSVVSLDFDATVAWPAYDWMGVS
                     KAALESVSRYLARDVGRRGVRVNCISAGPLRTVAAKGIPGFDRFIDDWHRRAPLGWDV
                     SDPGPVAGTALFLFSDLSRGISGELIHVDGGFHAMGTDLSE"
     gene            1989..>2766
                     /locus_tag="VM638_00345"
     CDS             1989..>2766
                     /locus_tag="VM638_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10908.1"
                     /translation="MRSRLPLLLLLSLLGPIAPVAAEGEVSGSATLAISPTTARAGRT
                     IALNGALEVAEPCLAGRAVVIEHLPATGAGWGSVAATATSADGSFLRTVLAQENGSFR
                     ARITAEERDGVACAEVVSPSVGVAVRAFVTARFPRPRLGAGGCAPLAIEVLPPKPGQA
                     VAVELRSGGSWTPLATLTLDDASTASFPRCHGWRSIGETFELRATWQQQDLAHVAGAS
                     SAAVLEVVKASWMRRLDRLTDGTSVSISVRDAFTTFRTAAE"
BASE COUNT          366 a         1018 c          992 g          390 t
ORIGIN      
        1 ccgatcaccg gccgcgagcg gccggctcat cccgtcggga tcgggagcgg ccgcaccccg
       61 agggcaaccg tcatggtctc gcggctgccg tcgcgccgca cgatcgagac ctggatccgc
      121 tcccccggct catgctcgac gatcgccgag cggaccgcct cgtcgtcggt gacgggtcgc
      181 ccgccgatgc cgatgatgat gtcgccgggt cggatccccg cccggtccgc cggaccaccc
      241 ggcgccacgt ccacgacgag ggccccctgg tcgaccggga ggttgagctg gaaggacagg
      301 ctcgagtcga cggttcgcgt cgagacgccg atgtaggcca cctcggcctc ggggtcgcgg
      361 atcgcgtcgc ggatgacttc cttcgcgcgg ttgatcgcga tcgcgaagct gatgttctcg
      421 gcggcgcccg cctgcacgcc cgccgtgttg atgcccacga cccgaccgct cagatcgacg
      481 agcggcccgc cggagttgcc ggggttgatg gcggcgtcgg tctggagcag gtcctcgtac
      541 gtgcgctcgc cgacctgcgg atcgctcgcg gtgatcgtgc gccccttcgc cgagatgatg
      601 ccggaggtga cggaagggcc gccttcgagc gcgagcgcga acccgagggc aacgaccgtt
      661 tcgccgaggc gcacgtggtc ggagtcgccg aggtccaccg tcgggaggtc atcggcctcg
      721 acctgcagga ccgcgaggtc ggcgttcggg tctcccccga tcacgcgcgc atcgaagcgg
      781 cggccgtcgt ccgtgatgac ccggatcgtc agccccccct ccacgacgtg gaagttcgtc
      841 acgatgatgc cgctctcgtc cacgatgaac ccggtcccgg tgcccccctg cgcctcgccg
      901 gtcagggccc cgggcgtcac gacgttcgtc gtgacgttca cgaccgccgg gcgcacggcc
      961 tcgacgacgc ggacgatcgt gtcgctgccc gccggcgccg ggggggcggg cgagaaggtc
     1021 gggccggtca ccggcagccc gaggtcctcc agcgccgcac aggacgtcag cagggccgtc
     1081 gcaaggaggg ccgcgagcga tctgcgcatg gcgcgaggat agcaatcggc cgcgcggtgg
     1141 ccgttcactc ggacaggtcg gttcccatcg cgtggaaccc gccgtcgacg tggatcagct
     1201 cgccgctgat gcccctcgag aggtcgctga acaggaacag cgccgtgccg gcgacgggcc
     1261 cagggtcgga cacgtcccac ccgagcggag cgcgacggtg ccagtcgtcg atgaaccggt
     1321 cgaacccggg gatccccttc gcggcgaccg tccgcagcgg ccccgcggag atgcagttca
     1381 cgcggacccc gcgccgtccg acgtcgcgcg ccaggtagcg cgacacggac tcgagggccg
     1441 ccttcgacac gcccatccag tcgtacgcgg gccaggcgac ggtcgcgtcg aagtcgagcg
     1501 agacgacgct cccaccgcgc accgacagca ggggcagcag cgggacgacc agctccttga
     1561 gcgagtacgc cgacgtccgg acggccgtgg ccacgctctc ccagggcgcg gtgaggaagt
     1621 tcccgccgag ggcgtcctcg ggggcgaagg cgatcgcgtg caggacgccg tcgagggcgt
     1681 cccatcgctc cccgagggcg gcggcgacct cttcgacctg ctccggacgg ttgacgtcga
     1741 gctcgagcac atcgcacggc tccgggaggc ggcgcgcgct cttctgcgtg atcgagagcc
     1801 cgcgcccgaa gccggtgagc acgacgtcgg cacccgcctc ctgagcctgt cgcgccacgg
     1861 cgaacgcgat gctctgcggc gtgaggacgc cggtgatgag cagccgcttc ccctcgagga
     1921 gcatcgccgt agcctagccg acgctcgccc cgcttgagcg agccgccacc gtcgccgatg
     1981 atgcggaggt gcgctcccgc cttcccctcc tcctcctcct ctccctcctc gggccgatcg
     2041 cgcccgtcgc ggccgagggt gaggtcagcg gctcggccac cctcgccatc tcccccacga
     2101 ccgctcgagc cgggcgtacg atcgccttga acggagccct cgaggtcgcg gagccgtgcc
     2161 tcgccgggcg ggccgtggtg atcgagcacc ttccggcgac cggagcgggc tggggatcgg
     2221 tcgcggcgac cgccacctcg gcggacggat cgtttcttcg gaccgtcctc gcgcaggaga
     2281 acgggtcgtt ccgtgcccgg atcaccgccg aggagcgaga cggtgtggcg tgcgccgagg
     2341 tcgtctcccc gagcgtcggc gtcgccgttc gcgcgttcgt gaccgcgcgg ttccctcgtc
     2401 cgcgcctcgg cgccggcggc tgcgcgccgc tcgcgatcga ggtcctcccg ccgaagccgg
     2461 gacaggcggt cgcggtcgag ctgcgatccg gcgggtcgtg gacgccgctg gcgacgctca
     2521 cgctggacga tgcctcgacg gcctccttcc cgcgttgtca cgggtggcgt tcgatcggcg
     2581 agaccttcga gctccgcgcg acgtggcagc agcaggatct cgcacacgtg gccggagcct
     2641 cttcggcggc ggtcctggag gtcgtgaagg cgtcctggat gcgccggctc gaccgcctga
     2701 ccgacgggac ctccgtgagc atctcggtcc gggacgcctt cacgaccttc aggaccgccg
     2761 cggaag
//
LOCUS       DATLGG010000014         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_190405, whole genome shotgun sequence.
ACCESSION   DATLGG010000014 DATLGG010000000
VERSION     DATLGG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190405"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1426
                     /locus_tag="VM638_00350"
     CDS             356..1426
                     /locus_tag="VM638_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10909.1"
                     /translation="MRETTVVLGLEDEALQEAVLHYLDRRPHVRVVGTEAEGAALARR
                     ISEARPKAAVVSPEVLREAPDLDGAALLVVTERETTEALRTAIRSGARGFYLWPEERE
                     SLGRETERLGLVDAPRAETSGRVVSIFGPRGGAGTTFLATHLAAACVDHASSPALVDL
                     DAFYNDVGSALGVPSDAPTAAALAPVMGEVTAEHLERVLYRHPGGFRVLLGPADPTAL
                     SAEELTATVGAVRTVCDPVILHLPRSMDVARGAIEASDLVLVVVTLDVLAFRAAKRAL
                     DHLRELGLDRRCRLVVNRAAPAEVAPEDVERVFGMPPVAVLRRDPAVLRAQNRGELLL
                     RRSGPLGRRVRELAGWICGGGA"
     gene            1402..>2134
                     /locus_tag="VM638_00355"
     CDS             1402..>2134
                     /locus_tag="VM638_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVM10910.1"
                     /translation="MDLRGRRMSLRWHPAGARTRLRTLVAERVRERLDVEALGRCAAA
                     ERRLRVREETLSVLAEQRAVLPARELNALINDISDELVGFGPIESLLKDPEVTEVMVN
                     GADDVYVERKGRLERVGHGLFEGEESVHHLIERIVGPLGLRVDESCPYVDARLPDGSR
                     LHAVIPPLSLCGPVVTIRKFGVTALGPDDLLRLGSLTEPMLGELARAVEGRANILVSG
                     GASSGKTTLLGALAGLIPAEERIVTI"
BASE COUNT          240 a          765 c          813 g          316 t
ORIGIN      
        1 ggtcctcatc gctcgtcgcg acggcgaccg gcgccagcgc acggtgctgc tcctcgtgat
       61 gggatcctcg gccggcggga tcgtcggcat cctctccccg ctgggagtgg tcctcgggat
      121 ggcctccggg ctgggagcgg ccgtcgcctt cgccgcgctc acccgcaggc cgtaagggcc
      181 ccaggcgggg ccgccccgtc cccgaacggg ggacccacag gttccgccgc ggtcgccccc
      241 gtccgggcga tgcgccgccg cggcagccgg ccgtatcgtc gcctcggcgc gttcggcgca
      301 gcttccccca ccttccccag aggccttccc cgcctggttc gaccgaggag gcgtggtgcg
      361 cgagacgacg gtggtgctgg ggctggagga cgaggcgttg caggaggcgg tcctgcatta
      421 cctcgaccgg cgcccgcatg tccgcgtcgt cggcacggag gcggaaggcg ccgcgctcgc
      481 gaggcgcatc agcgaagccc ggccgaaggc cgcggtcgtc tcccccgagg ttctgcgcga
      541 ggcgccggac ctcgacggcg ccgccctcct ggtcgtgacg gagcgcgaga cgacggaggc
      601 gctccggacg gcgatccgct cgggcgcgcg tgggttctac ctgtggccgg aagagcggga
      661 gtcgctgggt cgagagaccg agcggctggg gctcgtcgac gcgccccgcg ccgagacctc
      721 cggccgcgtc gtgtcgatct tcggcccacg gggtggtgcc gggacgacgt tcctcgcgac
      781 gcatctcgcg gccgcctgtg tcgaccacgc gtcctcaccg gccctcgtgg acctcgacgc
      841 cttctacaac gacgtcggaa gcgcgctcgg tgtgccgtcg gacgcgccca cggcggccgc
      901 gctcgccccg gtcatggggg aggtcacggc cgagcacctc gagcgtgtcc tgtaccgcca
      961 ccccgggggc ttccgcgtgc tgctcgggcc cgcggacccg acggccctct ccgcggagga
     1021 gctgaccgcg acggtcgggg cggtccggac cgtgtgcgac cccgtgatcc tgcatctccc
     1081 gcggtcgatg gacgtggccc gtggggcgat cgaggcgtcg gacctcgtgc tcgtcgtcgt
     1141 gaccctcgac gtgctggcgt tccgcgcggc gaagcgcgcg ctcgaccatc tgcgggagct
     1201 cggcctcgac cgccggtgcc ggctcgtcgt gaaccgcgcg gcgccggcgg aggtcgcacc
     1261 ggaggacgtc gagcgggtct tcggcatgcc gccggtcgcg gtgcttcgcc gggacccggc
     1321 cgtcctgcgc gcgcagaacc gcggcgagct gctgctgcgg cgatcgggcc cgctgggccg
     1381 gcgcgtccgc gagctcgcgg ggtggatctg cgggggcggc gcatgagcct gcgatggcat
     1441 ccggcagggg cgcggacgcg acttcggacg ctcgtcgcgg agcgcgtccg ggagcggctc
     1501 gacgtcgagg cgctcggtcg ttgcgccgcc gcggaacgtc ggctgcgcgt ccgggaagag
     1561 accctctccg tcctcgcgga gcagcgggcg gtccttcccg cgcgggagct gaacgcgttg
     1621 atcaacgaca tctccgacga gctcgtcggc ttcggtccca tcgagtcact cctgaaggac
     1681 ccggaggtga ccgaggtgat ggtgaacgga gccgacgacg tctacgtgga gcggaagggg
     1741 cggctcgagc gggtcggaca cgggcttttt gagggggagg agtccgtgca ccacctgatc
     1801 gagcggatcg tggggccgtt gggtctccgg gtcgatgagt cgtgcccgta cgtggacgcc
     1861 cgacttccgg acggctctcg gcttcacgcc gtgataccgc ccctttcgct ctgcggcccc
     1921 gtcgtcacga tccggaagtt cggcgtcacg gcgctcgggc cggatgacct cctccgtctc
     1981 ggctcgctga ccgagccgat gctcggcgag ctcgcgcggg ccgtggaggg tcgcgcgaac
     2041 atcctcgtct ccggcggcgc gtcatccggc aagacgacgc tcctcggcgc cctcgcgggg
     2101 ctgatcccgg ccgaggagcg gatcgtgacg atcg
//
LOCUS       DATLGG010000015         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_190472, whole genome shotgun sequence.
ACCESSION   DATLGG010000015 DATLGG010000000
VERSION     DATLGG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190472"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VM638_00360"
     CDS             complement(<1..539)
                     /locus_tag="VM638_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10911.1"
                     /translation="MEKRVFGILLLMALTGCAGGVDSAATADDDDDTTSPGYKLTDGS
                     YQYVVDEVPNDTCWAPPKTHPSVPMTVNAEVVVEGNIVHVTPDPVGGVSQTFDITRSG
                     DSLSGASAGNVDLNSQGLDCILHIESTLTGAVTDDDQFDATIVLNVSEAGGTLCGFLV
                     GSLDANQLDQLPCSLTLAGT"
     gene            complement(716..>2343)
                     /locus_tag="VM638_00365"
     CDS             complement(716..>2343)
                     /locus_tag="VM638_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVM10912.1"
                     /translation="PRVLGPMSTVEALDEVSARGSSDWSRYVRLRVVRNLTDVIRRWF
                     RVEITFADETGYVRAFPGNRIFEPTNPVCRQVLADREGFRRCGGSSAEAHGLVRRIGR
                     PGDRRPRPAESVCHAGFRQIVIPVEVGGRVVGSVIAGGILPDADPEEIVARVSALGID
                     AESVRQALPHVPRIDELSLQYLLELVALVVQEVVRFHGELAEKEAALARLSQELQGKY
                     GFGAIIGQSRPIREMLATLEKVCASDSTILVQGENGTGKELIARAIHWNSPRRDKPFV
                     TQSAAAFNDNLLESELFGHVKGAFTGAIKDKKGLLEAADGGTLFLDELGEMTAAMQVK
                     LLRVLQEGTFMPVGSTETKRVDIRLVCATNRDLRRMVERGEFREDLYYRINVINVHVP
                     PLRDRREDIPLLVRHFLKQLAVEKKTTPKEVSDEVLERFYDYEWPGNIRELENEIERL
                     VVMTGDEPRVTAELLSQRIKDRSMRANVRGVRVEKTLDQILEEVERRVLMEGLRKHRW
                     NKTQLARQLGISRKGLIAKVQRYQLDRRREPRA"
BASE COUNT          320 a          810 c          829 g          384 t
ORIGIN      
        1 gtccccgcga gcgtgagaga gcaggggagc tggtcgagct ggttcgcgtc gaggctgccg
       61 acgaggaagc cgcacagcgt gccgcccgcc tcgctcacgt tcagcacgat cgtcgcgtcg
      121 aactggtcgt cgtccgtcac cgcgccggtg agggtgctct cgatgtgcag gatgcagtcg
      181 agcccctgcg agttcaggtc gacgttcccg gcgctcgcgc cggagaggga atcgccgctc
      241 cgcgtgatgt cgaaggtctg gctgacgccg ccgacggggt cgggggtgac gtgcacgatg
      301 ttcccctcga ccaccacctc cgcgttgacg gtcatcggga cggaggggtg cgtcttcggc
      361 ggcgcccagc aggtgtcgtt cggcacctcg tccacgacgt actgatacga gccgtcggtg
      421 agcttgtagc cggggctcgt cgtgtcgtcg tcgtcgtccg cggtcgcggc ggagtcgacg
      481 cccccggcgc agccggtgag cgccatcagc aggaggatcc cgaagactct cttctccatg
      541 gccgccgtcc gtgtgcaacg gcgaggcctt gtccggagtc atggaattcc gcgacgttgc
      601 gccgacttcc ggtggcgtac ggctgtccaa cgcctggtca cctgcgccgc gatgtcgcag
      661 cccgcccccg cgcgtgatgc aacacacgca actccgaccc ggttctcgtg cggggtcagg
      721 cgcggggttc gcggcggcgg tcgagctggt agcgctggac cttcgcgatg aggcccttgc
      781 ggctgatgcc gagctggcgg gcgagctggg tcttgttcca gcggtgcttc cggaggcctt
      841 ccatgaggac ccggcgctcg acctcctcga ggatctggtc gagggtcttc tcgacgcgca
      901 cgccgcggac gttcgcccgc atggagcggt ccttgatgcg ctgcgagagc agctccgccg
      961 tcacgcgcgg ctcgtcgccg gtcatcacca cgagccgctc gatctcgttc tcgagctcgc
     1021 ggatgttgcc gggccactcg tagtcgtaga agcgctcgag gacctcgtcg gagacctcct
     1081 tcggcgtcgt cttcttctcg acggcgagct gcttcaggaa gtgcctcacc aggagcggga
     1141 tgtcctcgcg ccggtcgcgc agcggcggca cgtgcacgtt gatcacgttg atgcggtagt
     1201 agaggtcctc gcggaactcg ccgcgctcga ccatccgccg caggtcgcgg ttcgtcgcgc
     1261 acaccaggcg gatgtcgacc cgcttcgtct ccgtggagcc gacgggcatg aaggtcccct
     1321 cctgcaggac gcggaggagc ttcacctgca tggcggcggt catctcgccc agctcgtcga
     1381 ggaacagcgt gccgccgtcg gccgcctcga gcagcccctt cttgtccttg atcgcgccgg
     1441 tgaaggcgcc cttcacgtgg ccgaagagct cgctctcgag gaggttgtcg ttgaaggcgg
     1501 ccgccgactg cgtcacgaag ggcttgtcgc gccgcggcga gttccagtgg atcgcgcgcg
     1561 cgatgagctc cttgccggtg ccgttctcgc cctgcacgag gatcgtggaa tcgctcgcgc
     1621 agaccttctc gagcgtcgcg agcatctcgc ggatcggccg cgactggccg atgatggcgc
     1681 cgaagccgta cttcccctgc agctcctgcg agaggcgcgc gagcgccgcc tccttctcgg
     1741 cgagctcgcc gtggaagcgg acgacctcct gcacgacgag cgcgacgagc tcgaggaggt
     1801 actgcagcga cagctcgtcg atccgcggga cgtgggggag cgcctggcgg acgctctccg
     1861 cgtcgatccc gagggccgag acgcgcgcga cgatctcctc cgggtccgcg tcgggcagga
     1921 tcccgccggc gatcaccgag ccgacgacgc ggccgccgac ctcgacgggg atcacgatct
     1981 ggcgaaagcc cgcgtggcag accgactccg cggggcgggg gcgccggtcg ccggggcggc
     2041 cgatccggcg cacgagcccg tgcgcctcgg cggaggagcc cccgcagcgc cggaacccct
     2101 cccggtcggc gaggacctgg cggcagacgg ggttcgtcgg ctcgaagatc cggttgccgg
     2161 gaaaggcccg gacgtagccg gtctcgtcgg cgaacgtgat ctccacccgg aaccagcggc
     2221 ggatgacgtc cgtcaggttc cgcacgaccc ggagccggac gtagcggctc caatcgctcg
     2281 aacccctcgc gctcacctcg tccagtgctt cgaccgtcga catgggaccg agcactctag
     2341 ggc
//
LOCUS       DATLGG010000016         4955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_96188, whole genome shotgun sequence.
ACCESSION   DATLGG010000016 DATLGG010000000
VERSION     DATLGG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96188"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VM638_00370"
     CDS             <1..315
                     /locus_tag="VM638_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HVM10913.1"
                     /translation="ARARDLFARLPLILDADAWVALPGGAGTLAEVALCWNVLQYDRP
                     VGRPLVLVGDRWRHSFDALREHLVVVDPADHDLVGRVETPAEALGLVMEAMAQAKNSS
                     TA"
     gene            complement(288..677)
                     /locus_tag="VM638_00375"
     CDS             complement(288..677)
                     /locus_tag="VM638_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVM10914.1"
                     /translation="MTHPNEDLARGASEALAKGDMETFLDFHSDDVVVRVQGRNPSAG
                     EFRGREEILRSAEQLTSLLDEPPTWETHDVMAGDDHAIVLGTQRFVRGGKTLEDHQVV
                     IAHIREGKANEIWVCSTDPYAVDEFFA"
     gene            815..3391
                     /locus_tag="VM638_00380"
     CDS             815..3391
                     /locus_tag="VM638_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10915.1"
                     /translation="MRRIRSVLAAALVAASLVTPGASAQQPEGLTASDNVRFVTNLKF
                     QNKYPQSSRRSVDMDFTTTEVVKLSEGATGDPVCVVDEGQADGCARDDAGEIVYQTEE
                     REFAYVGTYMNGLQTVDITDPENPVVVAVYDCAIAQSDVDVFHRPDLGRTFLTYSSDE
                     ISGQTNFSSRCHRDNGVMPGNYGAFIVDITDPRKPESVSFIAFPRGSHQLSVHPGGRW
                     VYSSPGALVNAQGAFHVSDVTDPEKPGPAVTVPLVTGLDAHDIIFGEGGNRAYVAALS
                     HTVIMDTTNPGKPVAIGRILDPTVNIHHEAHPYTTVDELTGIRHTFLLLSDEFAGASG
                     NEVCPGGGIHVYDITGHLERAPVKVGLYLMPHVGRAEGAPQGTAGLRRCTAHVMQMHP
                     EHDIATVAWYAAGTRVLDLSGILGASAGVTEQTGSQGAGIKEIGYARFNDGDVWATKT
                     NRIEDDGTFYLYSADTARMLDVFHVDLGRKPAGVSLSPGGGFTTRNVEWVRNIAAHAG
                     TSGGTRVGDYYYMTDPRGVYIYDISNPALPVPVGALPAIQSTTHVVFAQEEPETNGKI
                     LLVNAIAPGPSGAPPANGWLVVVDVRNKSAPTVVGSLNLYDHTWSCVLDCTYAIGRTG
                     PIVDLRDPANPVQVGNWRNQVSGASYMHDFTEVRPGYLIGSGQPTFYLDMTEPPVVKE
                     IARISPGFHSLGYHGAAWANDGTDPLLLMGAEVAPAGTTNLAGSDCTGTDVHAVATYD
                     ATELVDAPAGRKTFRKLDEWRVSGRGTYTDGKAPAHVLYCGHWFDPHPSWDGGGVLAT
                     AHYNWGTRFLRVGKDGKMREIGWFTPIGGDAGSAKWITDDILYVHDYRRGLDVLRFTG
                     RP"
     regulatory      3500..3702
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3830..4777
                     /locus_tag="VM638_00385"
     CDS             3830..4777
                     /locus_tag="VM638_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM10916.1"
                     /translation="MPRFVRRYLVAPMAVALGLVAAACGDTQPTPEAAPTPTGEAAFP
                     VTITAANGDVTIGARPERIVSMSATATEMLFAVDAGEQVVAVDSTSNFPPEAPTTDLS
                     AFEPNPEAVAELDPDLVIVSDDINEIIASLEALEISVIHQPAAATLEDTYAQIEQIGA
                     ATGNVPEAADLVASMRSEIERLSASVPAFEEPPTYYHELDQTFFSVTSETFIGQIYAL
                     AGLENIADGAADSGPYPQLSAEFIVDADPDLIFLADTKCCGMTVEKAARRPGWDRLTA
                     VERGAVVELDDDVASRWGPRVVDLLRIVVESVSELERAA"
     gene            4774..>4955
                     /locus_tag="VM638_00390"
     CDS             4774..>4955
                     /locus_tag="VM638_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006552926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVM10917.1"
                     /translation="MSAAPAAAGVRPMRLRPRLLLVAAVCLFGAVLVGVLVGPVPLRP
                     AAVLRDLAARLPLVDLP"
BASE COUNT          757 a         1760 c         1690 g          748 t
ORIGIN      
        1 gcgcgggccc gcgacctctt cgccagactc ccgctgatcc tcgacgccga cgcctgggtc
       61 gccctgcccg gcggagcggg gacgctcgcc gaggtcgcgc tctgctggaa cgtcctccag
      121 tacgaccgcc cggtcgggcg acctctggtc ctggtcgggg atcgctggcg acactccttc
      181 gacgcgctcc gcgagcatct cgtggtcgtc gatcccgcgg accacgacct cgtcggccgg
      241 gtcgagacgc ccgccgaggc gctcggcctc gtgatggagg cgatggctca ggcgaagaac
      301 tcgtcgaccg cgtaggggtc ggtcgagcac acccagatct cgttcgcctt cccctcgcgg
      361 atgtgcgcga tcaccacctg gtggtcctcg agcgtcttgc cgccgcggac gaagcgctgg
      421 gtcccgagca cgatcgcgtg gtcgtcgccg gccatcacgt cgtgcgtctc ccaggtcggc
      481 ggctcgtcga gcaggctcgt cagctgctcg gccgagcgga ggatctcctc gcggccacgg
      541 aactccccgg cgctcgggtt gcgtccctgg acccggacga ccacgtcgtc gctgtggaag
      601 tcgaggaacg tctccatgtc tcccttcgcg agcgcctcac tcgccccgcg tgccaggtcc
      661 tcgttcggat gcgtcatatc gccctccttc ggtcgactgc acgccgaccc taccccgccg
      721 aggaggccgt agcgcatccg gaccttccgg gaggaatccg gccacccgat gtcgaaccaa
      781 tgggcaggaa gcgatccttg agaggtgatc atccatgcgt cggatccgat ccgtgctcgc
      841 ggcggccctc gtggcggcct cgttggtgac tccgggagcc tcggcccagc agcccgaggg
      901 gctcacggcg agcgacaacg tccggttcgt caccaacctg aagttccaga acaagtatcc
      961 gcagtcctca cggcggtcgg tggacatgga cttcaccacc accgaggtgg tcaagctgtc
     1021 cgagggcgcc accggcgacc cggtgtgcgt ggttgacgag gggcaggccg acgggtgcgc
     1081 ccgcgacgac gccggcgaga tcgtctacca gaccgaggag cgcgagttcg cctacgtcgg
     1141 cacctacatg aacggcttgc aaacggtgga catcaccgat ccggagaacc cggtggtggt
     1201 agcggtgtac gactgcgcga tcgctcagtc cgacgtcgac gtgttccacc ggccggacct
     1261 cggccgcacc ttcctcacct acagctccga cgagatctcg ggccagacga acttcagctc
     1321 tcgctgccac cgggacaacg gcgtgatgcc cgggaactac ggggcattca tcgtggacat
     1381 caccgacccg cgcaagccgg agagcgtgag cttcatcgcg tttccccgtg ggtcacacca
     1441 gttgagcgtg caccccggcg ggcgctgggt ctacagctcc ccgggcgcgc tcgtgaatgc
     1501 ccagggggcc ttccacgtct ccgacgtgac cgacccggag aagcccggac ccgcggtcac
     1561 cgtccccctg gtgacgggcc tggacgccca cgacatcatc ttcggtgaag gtgggaaccg
     1621 cgcctacgtc gccgccctgt cccacaccgt gatcatggac acgacgaacc ccggcaagcc
     1681 ggtcgcgatc ggacggatcc tcgatcccac cgtgaacatc caccacgagg cgcaccccta
     1741 cacgaccgtg gatgagctca ccgggatccg ccacaccttc ctgctcctct ccgacgagtt
     1801 cgccggtgcg agcggcaacg aggtatgccc cggcggcggc atccacgtct acgacatcac
     1861 cgggcacctg gagcgcgcgc cggtcaaggt gggcctgtac ctcatgcccc atgtgggccg
     1921 tgccgagggg gccccgcagg gaaccgccgg ccttcgccgg tgcacggcgc acgtgatgca
     1981 gatgcacccc gagcacgaca tcgccaccgt cgcctggtac gcggcgggca cacgggtgct
     2041 cgatctgtcg ggcatcctcg gcgcgtcggc cggcgtgacc gagcagacgg ggtcgcaggg
     2101 cgccgggatc aaggagatcg gctacgcgcg cttcaacgat ggagacgtgt gggccacgaa
     2161 gaccaaccgg atcgaggacg acgggacctt ctacctctat tcggccgaca ccgcccgcat
     2221 gctcgatgtg ttccacgtcg acctggggcg gaagccggcc ggcgtgtccc tctcgcccgg
     2281 cggcggattc accacccgca acgtcgagtg ggtccgcaac atcgccgcgc atgccgggac
     2341 ttccggtggg acgcgcgtgg gggactacta ctacatgacc gatcctcgcg gcgtgtacat
     2401 ctacgacatc tccaacccgg ccttgccggt gccggtgggc gcgcttccgg ccatccagtc
     2461 gacgacccac gtggtgttcg ctcaggagga acccgagacc aacgggaaga tcctgctggt
     2521 gaatgccatc gcgccggggc ccagcggggc tccgccggcg aacggatggc tggtggtggt
     2581 ggacgtgcgc aacaagtccg cacccacggt cgtgggctcc ctgaatctgt acgaccacac
     2641 ctggtcgtgc gtgctggatt gcacgtacgc gatcgggcgg accgggccga tcgtcgacct
     2701 gcgcgacccc gcgaaccccg tgcaggtcgg gaactggcgg aaccaggtat cgggcgccag
     2761 ctacatgcac gacttcaccg aggtccgtcc gggctacctg atcggatccg ggcagcccac
     2821 gttctacctg gacatgacgg agccgccggt cgtcaaggag atcgcccgca tcagccccgg
     2881 cttccactcc ctcggctacc acggtgcggc gtgggcgaac gacggaaccg acccgctgct
     2941 gctgatgggc gcggaggttg ccccggcggg caccacgaac ctcgcgggca gcgactgcac
     3001 gggcacggac gtccacgcgg tggccaccta cgacgccacc gagctggtcg acgcgccggc
     3061 cggccgcaag acgttccgca agctggacga gtggcgggtg tcgggtcgcg ggacctatac
     3121 ggacggcaag gcgccggctc acgtgctgta ctgcggccac tggttcgacc cgcatccgtc
     3181 ctgggacggc ggcggggtgc tggccacggc ccactacaac tgggggacgc ggttcctgcg
     3241 ggtgggcaag gacggcaaga tgagggagat cggctggttc accccgatcg gcggcgacgc
     3301 cggatcggcc aagtggatca ccgatgacat cctctacgtc cacgactacc gccgcggact
     3361 cgacgtcctc cgcttcaccg gacgccccta gcgcaccgag aggctggcgg accgaaggca
     3421 cgctcctcgg cgttcttcgg acggtggagc ctgggcgtgt tccgggttgc ctccgcgttc
     3481 ggcgtcggca agaatcggca cgcgaagaac gacccgaggt ggccccggcg cggacggcat
     3541 cccgccgggg ctgaaaaggg aagccggtgc gagtccggca cggtcgcgcc actgtgaccg
     3601 gggagcgaac ccgagaaatg ccactgggac cgatggtcct gggaaggctc ggggagcggt
     3661 gatccgggag tcaggagacc tgcctcgggg tcgaggatgc cgtccctcgc gcgaaggcga
     3721 cgggcccggg gccgcacgtc tccggccgag atcggaggcg gttcccgttc cctagccctc
     3781 cgcggcgggc ggccgagcgg ccgcgcgttc ggccggagag gagggaacga tgccacgctt
     3841 cgtccgccgc tatctcgtcg ccccgatggc ggtcgccctt ggcctcgtcg cagcagcctg
     3901 tggtgacacc cagccgaccc ccgaagccgc accgacgccg acgggggagg cggcgttccc
     3961 ggtcacgatc acggcggcca acggcgacgt gacgatcggg gctcgccccg agcgcatcgt
     4021 gtcgatgtcc gccaccgcga ccgagatgct gttcgcggtc gacgccggcg agcaggtggt
     4081 cgcggtcgat tccacctcga acttcccgcc ggaggctccg acgacggacc tctccgcctt
     4141 cgagccgaac cccgaggccg tcgccgagct ggaccccgat ctggtcatcg tctccgacga
     4201 catcaacgag atcatcgcct cgctggaggc gttggagatt tcggtgatcc accaaccggc
     4261 cgccgcgaca ctcgaggaca cctacgcgca gatcgagcag atcggtgccg cgaccgggaa
     4321 cgtgccggag gcggccgacc tcgtggcgtc catgcggtcg gagatcgagc ggctctcggc
     4381 ctcggtcccc gcgttcgagg agccgcccac ctactaccac gagctggacc agaccttctt
     4441 ctccgtgacc tcggagacgt tcatcgggca gatctacgcg ctcgccggcc tggagaacat
     4501 cgccgacggg gcggcggaca gcgggccgta cccccagctg tccgcggagt tcatcgtcga
     4561 tgccgatccc gacctcatct tcctcgcgga cacgaagtgc tgcggcatga cggtcgagaa
     4621 ggccgcaaga cggccgggct gggaccgcct caccgcggtg gaacgaggag cggtggtgga
     4681 gctcgacgat gacgtcgcct cgcgttgggg cccgcgcgtc gtggatctcc tccgcatcgt
     4741 ggtcgagagc gtctcggaac tcgagcgggc cgcatgagcg cggccccggc ggccgctggg
     4801 gtccgtccga tgcggcttcg gccgcgcctc ctgttggtcg ctgcggtctg cctcttcggt
     4861 gccgtgctcg tcggggtcct cgtcgggccg gtgccgctcc ggcccgcggc ggtcctgcgg
     4921 gacctcgccg cacgtctccc gttggtcgac cttcc
//
LOCUS       DATLGG010000017         2711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_80316, whole genome shotgun sequence.
ACCESSION   DATLGG010000017 DATLGG010000000
VERSION     DATLGG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80316"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="ribD"
                     /locus_tag="VM638_00395"
     CDS             <1..711
                     /gene="ribD"
                     /locus_tag="VM638_00395"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HVM10918.1"
                     /translation="ERQNRAFLTHARTGRPFVSLKMAASLDGKAAARDGSSRWITGPQ
                     ARADVQRMRAAADAVLVGAGTALRDDPSLTVRDPGYTGDPKLRVVVDGRGIVPPSHRL
                     FRDGAAPTLVATTEGAPGERRDAWRAAGAEVLVLDDAGSGLIPLDALLSELGKRDVQH
                     VLLEGGPTLAWEAVASGVVHEVVVFIAPILVGGEDAPSVLMGGGMPTIGDAVRLRIRE
                     VTRVGEDTKVVADVHRDR"
     gene            692..1288
                     /locus_tag="VM638_00400"
     CDS             692..1288
                     /locus_tag="VM638_00400"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVM10919.1"
                     /translation="MFTGIVEHLGVVRSLARDGEGARLEVEAGPVGERATLGASVAVD
                     GVCLTVVEREGGRLRFDLAEETLGRTTLGELTPGARVNLEGPLTLSSPLGGHLVQGHV
                     DGVGSVVAVEGGPSGTRMRVAATEAMARYLVEKGSVAVDGVSLTVAATDGAGTFEVAL
                     VPHTLEVTTLGARRTGDRVNLEVDIVAKYVERLVREDR"
     gene            1285..2526
                     /locus_tag="VM638_00405"
     CDS             1285..2526
                     /locus_tag="VM638_00405"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HVM10920.1"
                     /translation="MKEDVRTEVFAAVEDAVEDIREGKIVIVVDDADRENEGDLILAA
                     EKVTPEAITFMVRHTSGVICMPIVGERLDELRLPLMVTDSNDVQRTAFTVSVDVIEGT
                     TTGISAADRAATIRALVDPRTRASDLQRPGHIFPLRYREGGVLKRAGHTEAAVDLARL
                     AGLYPAGVLCELVNDDGSMARLPDLIRFAEEHGLKILSIADLIEYRRRRERLVTPVAE
                     ARIPTIYGEFRAHAYESLVDGRTHVAFVMGEVADGEDILVRVHSECLTGDVFQSRRCD
                     CGTQLDDALSKISAEGRGVVLYIRGHEGRAIGLTHKLRAYRLQEDGRDTVEANLELGF
                     PPDSRDYGIGAQILYDLGVRTMRLLTNNPSKRAGLEGYGLSIVDRIPLETEPTAENLE
                     YLRAKREKLGHLLSNLEEAER"
     gene            2523..>2711
                     /locus_tag="VM638_00410"
     CDS             2523..>2711
                     /locus_tag="VM638_00410"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVM10921.1"
                     /translation="MRVHEGTLEARGRRFAIAAASFNRIVTDRLVEGATAAFRRHGVS
                     EDDLELAWAPGSFELPLVA"
BASE COUNT          389 a          911 c         1034 g          377 t
ORIGIN      
        1 gagcggcaga accgcgcctt cctcacgcac gcgcggaccg gtcgcccctt cgtctcgctg
       61 aagatggccg cgtccctcga tgggaaggcg gcggcgcgcg acggatcctc ccgctggatc
      121 accggcccgc aggcgcgtgc cgacgtccag cggatgcgcg cggcggcgga cgccgtcctc
      181 gtgggagcgg ggaccgctct ccgcgacgac ccctcgctga cggtgcgtga tcccgggtac
      241 acgggggacc cgaagctccg ggtcgtcgtg gacggccggg ggatcgtgcc gccctcgcat
      301 cggctgttcc gcgacggcgc cgcgcccacg ctcgtcgcga cgaccgaggg tgcgcccggt
      361 gagcggcgtg acgcctggcg cgcggccggc gccgaagtcc tcgtcctcga cgatgccggc
      421 tcggggttga tccccctcga cgcgttgctc tcggagctcg gcaagcgcga cgttcagcac
      481 gtgctcctcg aaggggggcc caccctcgcg tgggaggcgg tcgcgagcgg ggtcgtccac
      541 gaggtcgtgg tcttcatcgc gccgatcctc gtggggggcg aggatgcccc ctcggtgctg
      601 atggggggcg ggatgccgac gatcggggac gccgtccggc tccgcatccg cgaggtgacg
      661 cgcgtgggcg aggacacgaa ggtggtggcg gatgttcacc gggatcgttg agcatctcgg
      721 cgtcgttcgg tcgctcgcga gagacggcga gggcgcgcga ctcgaggtcg aagcggggcc
      781 ggtcggtgag cgggcgacgc tcggggcctc cgtggccgtg gacggggtct gcctcacggt
      841 cgtcgaacgg gaggggggcc ggctgcgctt cgacctcgcg gaggagaccc tcgggcgcac
      901 gacgctcggc gagctcacgc cgggggcgcg cgtgaacctc gaggggccgc tgacgctctc
      961 ctcgccgctc ggcggccatc tcgtgcaggg tcacgtcgac ggggtgggaa gcgtcgtggc
     1021 ggtcgaggga ggaccgtccg ggacgcggat gcgcgtcgcc gcgacggagg ccatggcgcg
     1081 atacctggtg gagaaagggt cggtcgcggt cgacggcgtc agcctgaccg tcgccgcgac
     1141 cgatggggcg ggaacgttcg aggtcgcgct cgtcccgcac acgctggagg tgacgacgct
     1201 cggcgcgcgg cgaacgggtg accgggtgaa cctggaggtg gacatcgtgg cgaagtacgt
     1261 cgagaggctc gtgcgggagg accgatgaag gaggacgtgc ggaccgaggt cttcgcagcg
     1321 gtcgaagacg cggtcgagga catccgagaa gggaagatcg tgatcgtcgt cgacgacgcc
     1381 gatcgcgaga acgagggcga cctgatcctg gcggcggaga aggtcacgcc cgaggcgatc
     1441 acgttcatgg tccgccacac gagcggcgtg atctgcatgc cgatcgtcgg cgagcgtctg
     1501 gatgagctcc gccttccgct gatggtcacc gacagcaacg acgtgcagcg caccgcattc
     1561 acggtctccg tcgacgtgat cgagggcacc accacgggca tctctgcggc cgaccgtgcc
     1621 gcgacgatcc gggccctcgt cgacccgagg acgcgcgcga gcgacctcca gcggccgggg
     1681 cacatcttcc cgctccgcta ccgcgagggg ggcgtgctca agcgcgccgg ccacaccgag
     1741 gccgccgtcg acctcgcgcg cctcgcgggg ctctacccgg ccggggtcct ctgcgagctg
     1801 gtcaacgacg acggctcgat ggcccgtctc cccgacctga tccgtttcgc ggaggagcac
     1861 gggctgaaga tcctctcgat cgccgatctg atcgagtacc gccgccgccg cgagcggctc
     1921 gtgacgccgg tcgcggaggc acgcatcccg acgatctacg gcgagttccg cgcgcacgcc
     1981 tacgagagcc tcgtcgatgg gcggacgcac gtcgccttcg tgatgggcga ggtggccgac
     2041 ggcgaggaca tcctcgtgcg cgtccactcc gaatgcctca cgggcgacgt cttccagtcg
     2101 cgccgctgcg actgcgggac ccagctggac gacgcgctct cgaagatctc ggccgaggga
     2161 cgcggcgtcg tcctgtacat ccgcgggcac gaggggcggg cgatcgggct cacgcacaag
     2221 ctgcgggcgt accggctcca ggaggatggt cgcgacaccg tcgaggccaa cctggagctc
     2281 gggttcccgc ccgattcgcg ggactacggg atcggggccc agatcctcta cgacctcggg
     2341 gtgcggacga tgcgcctgct gacgaacaac ccgtcgaagc gcgcaggcct cgaaggctac
     2401 ggcctctcga tcgtcgaccg gatccccctc gagaccgagc cgaccgcgga gaacctcgag
     2461 tacctgcggg cgaagcggga gaagctcgga cacctgctgt cgaacctcga ggaggcggag
     2521 cggtgagggt gcacgaggga acgctggagg ctcgcggccg gcgattcgcg atcgccgcgg
     2581 cgagcttcaa ccggatcgtg accgatcggc tcgtcgaggg ggcgacggcg gccttccgac
     2641 gccacggcgt ctccgaggat gacctcgagc tcgcatgggc gccggggtcc ttcgagcttc
     2701 ccctcgtggc g
//
LOCUS       DATLGG010000018         6992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_32927, whole genome shotgun sequence.
ACCESSION   DATLGG010000018 DATLGG010000000
VERSION     DATLGG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32927"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VM638_00415"
     CDS             <1..653
                     /locus_tag="VM638_00415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM10922.1"
                     /translation="IPELTGAVRNSLIVAAVAMVVATALGTLMALALTRYRFRGRGAT
                     NLLIFVPMATPEVVMGASLLSLFVTINIARGMTTILIAHIMFNVSFVVVTVRARTAGF
                     NREWEEAAKDLGASPWVAFRTVTFPLIFPGVLAAAVLAFALSIDDYVITLFNAGQTTT
                     FPLWVFGAARFGTPPQVFVMGTLIFVTGVALALGSVWFQRGRLHDAASPMPGARGS"
     gene            668..2023
                     /locus_tag="VM638_00420"
     CDS             668..2023
                     /locus_tag="VM638_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVM10923.1"
                     /translation="MDERMDQRAKEIATRERARLQERTPASRRLFERAVGSLPMGVAS
                     TFHADDPYPIYVGRGEGASVWDVDGNRYLDFHNGFGTMAVGHAHPKVVEAIERAARTG
                     THFAATTEAAVALAEELCRRFRLDRVRFVNSGTEATMDAVRIARAATGRDTIAKIAGS
                     YHGHHDTVMFSVVSNADMVGGRDLPPDRPMSKGIPPEMAKRTLVVPFNDADALQALFS
                     DRGEEIACLIMEPVMMNIGIALPQPGYLERVRELCTQHDVILIFDEVKSGATIAEGGA
                     TERFGVQPDLACFAKAIGGGVPAGAFGGRADIMEIIEHGAAQQGTFNGNPLAAHAGLA
                     ALTEVLTKDAYEHFADIGSRLADGCRRAIEDHGVPAHTVDLGCKGCVSYTEEPLRDYR
                     DFLGAHSALFEASWPWTVNRGVFMTPGDEEQWTLSVQHTEEDIDRYVDVFAAFCAELA
                     R"
     gene            complement(2020..3186)
                     /locus_tag="VM638_00425"
     CDS             complement(2020..3186)
                     /locus_tag="VM638_00425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVM10924.1"
                     /translation="MLRLRPLLVAFLVGLPALPAGAAGEVTLEAVPAASIPSGGTVTF
                     RGAVPSASDGDTVQIVNADDPTESLAPAATISGGAYDVVSVPLTRNVRAKAVWGLFES
                     EPLLVLVRPKLTIALSDVRLFDRALVRGTIDPPHDGQTVTVQLLRKGVVMREATATIS
                     DGRFRARLGVRKPGTYRAKVIFPADVDHAEASKRSDERTTPLPTLRTGSRNRFVRLLE
                     RRLRDLRYRVPAPDTHFDHRTADAVLAFRKVQGIRRTRIVTASTWRKLASPRKPRARS
                     SRPWFHIEVDQTKQVLYLVRGGRITSIVHVSTGGPGIGTTYDGTWRVHRRLAGYSPGR
                     LYYPAYFHGNRAIHGWPDVPPTPASHGCVRVPMWTAQWLYDQAPVGRVIKIYHS"
     gene            complement(3196..4365)
                     /locus_tag="VM638_00430"
     CDS             complement(3196..4365)
                     /locus_tag="VM638_00430"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HVM10925.1"
                     /translation="MKAYAAVFGASALTAFVTTPLVRRFAVRFGLIDRPSDRKVHPKP
                     TPTLGGLALWAGAVVGLGVAYALPQFGSLFEASSEPLGAVLAGTIVVALGAFDDIRGI
                     SAPAKAAGQVLAAGVLILAGVQFIYFWLPGLGILSLSPDLALPLTVIWVLVMVNAVNL
                     IDGLDGLAAGVVTIAAVAFFVYAEDAGAPLWDGPSPATLLTVIVAGVAIGFLPWNFNP
                     ARIFMGDSGSMLLGVLLAAATISGVGRTDAPTNPDFAALAIPVAIPLLVLAVPLTDVA
                     FAILRRIRRGRPVTRPDKEHIHHRLLEIGHSHRAAVLMMYLWSALLAGSALFITFIRS
                     RTLAVALIVGVGVILVGTSWPRMRRRNGRARGTSPALGSNGEVAGTSRDSVGRTR"
     gene            complement(4362..5618)
                     /gene="glyA"
                     /locus_tag="VM638_00435"
     CDS             complement(4362..5618)
                     /gene="glyA"
                     /locus_tag="VM638_00435"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVM10926.1"
                     /translation="MREWAADVEELERVDPEVADAIRGELERERSTIRLIASENYTSP
                     AVLAALSSSMNNKYAEGYPFRRYYGGCRWVDVTESLAIERAKELFGAEHANVQPHAGA
                     QANMAAYFAFMQPGDTLLGMSLPHGGHLTHGSPVNFSGKWFNVVPYELDKDTELIDMD
                     RVRDLAREHRPKVLVAGATAYPRTIDFAAFREIADEVGAIFMVDAAHFIGLVAGGAYP
                     SPVPHADVVTFTTHKTLRGPRGAMILCREQHAKAIDKTVFPMMQGGPIENAIAAKAVA
                     LKEAMAPGFRDYAERVVRSARALAAGLQDQGLRLVSGGTDSHLALVDLQPIGVTGADA
                     EAACDAVGITLNKNAIPYDPLPPMTTSGIRVGTPGPATLGMDEGEMKEIAETIGEVLR
                     APDDEAVRERGRARVRDLTSRFPAYP"
     gene            complement(5615..6067)
                     /gene="rpiB"
                     /locus_tag="VM638_00440"
     CDS             complement(5615..6067)
                     /gene="rpiB"
                     /locus_tag="VM638_00440"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HVM10927.1"
                     /translation="MRIAIGSDHAGYALKEDLKGFLKEQGHQVEDLGTHSEEAVDYPP
                     FCAAVGRAVRDGAADRGIVLGGSGQGEQIAANKVRGVRAALCNDLFTAALAREHNDAN
                     VLSIGARIVAPALAHAIVDRWLSTPFEGGRHVRRLDLIREIEDEEATT"
     gene            complement(6069..6620)
                     /locus_tag="VM638_00445"
     CDS             complement(6069..6620)
                     /locus_tag="VM638_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HVM10928.1"
                     /translation="MLRVLAVCTGNICRSPLAEAFSAACSDLVPGGLSVRSAGTWAND
                     GMPATRESRLAGRERGVDLEGHRSSRLTSAAIREADLILGMTAEHVEGILALDPSAAA
                     RTFTLKELAAILRGLPGTGDVRARLAEADRHRDPALVDDVADPLGLGMEIYRHIALEV
                     DVAVDEILAGLGAGTGAARTAEA"
     gene            complement(6663..>6992)
                     /locus_tag="VM638_00450"
     CDS             complement(6663..>6992)
                     /locus_tag="VM638_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HVM10929.1"
                     /translation="TAASEGWDLGDAGGTVGIRVPDHEMTLSLLARTGPLAVTSANRS
                     GEPTPDQCEELVAALGEGVAIYLCAGPARHKVPSSIVDLTHAPPRVIRHGAVPLEAIR
                     EVLGEVE"
BASE COUNT         1022 a         2462 c         2527 g          981 t
ORIGIN      
        1 cgatccccga gctcaccggc gcggtgcgca actcgctgat cgtcgcggcg gtcgcgatgg
       61 tggtcgcaac cgcgctcgga acgctgatgg ccctcgccct gacccgctac cggttccgag
      121 gccgcggcgc gacgaacctg ctgatcttcg tcccgatggc gacgccggag gtcgtgatgg
      181 gggcctcgct gctgtcgctg ttcgtcacga tcaacatcgc gcgcgggatg accaccatcc
      241 tcatcgccca catcatgttc aacgtctcgt tcgtcgtcgt gacggtgcga gcacgaacgg
      301 ctggcttcaa ccgcgagtgg gaagaggcgg ccaaggacct cggggcatca ccctgggtgg
      361 ccttccgcac ggtgacgttc cccctgatct tccccggggt cctcgcggcg gcggtcctgg
      421 cgttcgctct gtcgatcgac gactacgtga tcacgctgtt caacgcgggg cagacgacca
      481 cgttcccgtt atgggtgttc ggggcagcga ggttcggaac gccgccgcag gtgttcgtga
      541 tgggaacgct gatcttcgtg acgggcgtgg cgctggcgct cggcagcgtc tggttccagc
      601 gcgggaggct gcacgacgcg gcgagcccga tgccgggcgc ccgtggatcc tgacggaagg
      661 agcgagcatg gacgagcgta tggaccaacg agcgaaggag atcgcgacgc gggagcgggc
      721 gcgcctgcag gaacggacgc cggcgtcgcg tcggctcttc gagcgagcgg tgggttccct
      781 tccgatgggc gtcgcctcga cgttccacgc cgacgacccc tacccgatct acgtcggccg
      841 gggcgaagga gcatcggtct gggacgtcga cggcaaccgc tacctcgact tccacaacgg
      901 gttcgggacg atggcggtgg gacatgccca cccgaaggtc gtcgaggcca tcgagcgcgc
      961 ggcgcgcacc ggcacgcact tcgccgccac gacggaggcc gcggtcgcgc tcgcggagga
     1021 gctctgccgg cgcttccggc tcgatcgcgt ccggttcgtg aactccggga cggaggcgac
     1081 gatggacgcg gtcaggatcg cccgtgcggc gacgggccgc gacacgatcg cgaagatcgc
     1141 gggctcctac cacgggcacc acgacaccgt gatgttctcg gtcgtctcga acgccgacat
     1201 ggtcggcggc cgcgacctgc cgccggaccg gccgatgtcg aaggggatcc cgccggagat
     1261 ggcgaagcgc accctcgtcg tcccgttcaa cgacgccgat gcgctccagg cgctcttctc
     1321 ggatcggggt gaggagatcg cctgcctgat catggagccg gtgatgatga acatcggcat
     1381 cgcgctcccc cagcccgggt acctcgagcg cgtccgggag ctgtgcaccc agcacgacgt
     1441 catcctgatc ttcgacgagg tgaagagcgg cgcgacgatc gccgagggcg gagcgacgga
     1501 gcgcttcggc gtccagccgg acctcgcctg cttcgcgaag gcgatcggcg gcggcgtccc
     1561 ggccggagcc ttcggcgggc gtgccgacat catggagatc atcgagcacg gcgcggcgca
     1621 gcagggcacc ttcaacggga accccctggc ggcccacgcc ggcctcgcag cgctcaccga
     1681 ggtcctgacc aaggatgcct acgagcactt cgcggacatc ggatcgcgtc tcgcggacgg
     1741 atgtcgccgc gcgatcgagg atcacggggt cccggcgcac accgtcgacc tcggctgcaa
     1801 gggatgcgtc tcctacaccg aggagccgct ccgcgactac cgcgacttcc tcggcgctca
     1861 ctcggcgctg ttcgaggcgt cctggccgtg gaccgtgaac cgcggcgtgt tcatgacgcc
     1921 cggcgacgag gagcagtgga cgctctccgt ccagcacacc gaagaggaca tcgatcggta
     1981 cgtcgacgtc ttcgcggcgt tctgcgcgga gctcgcgcgc taggagtggt agatcttgat
     2041 gacccggccg acgggagcct ggtcgtagag ccactgcgcc gtccacatcg gcacgcggac
     2101 gcagccgtgg ctcgcgggcg tgggcggcac gtccggccag ccgtggatcg cgcggttccc
     2161 gtggaagtag gccggatagt agaggcggcc ggggctgtac cccgcgaggc gccggtgcac
     2221 gcgccacgtg ccgtcgtacg tcgtcccgat ccccgggccg ccggtcgaga cgtgcacgat
     2281 gctcgtgatc cggccgccgc ggaccaggta caggacctgc ttcgtctggt cgacctcgat
     2341 gtggaaccac ggccgggagc tgcgcgcccg gggcttccgg ggtgaggcca gcttccgcca
     2401 ggtcgaggcg gtcacgatcc gggtccgccg gatgccctgg acctttcgga acgcgagcac
     2461 cgcgtcggcc gtccgatggt cgaagtgcgt gtccggcgcc ggcacgcggt accggaggtc
     2521 gcggagacga cgctcgagga ggcggacgaa ccggttgcgc gagcccgtcc gaagggtggg
     2581 cagcggcgtg gtgcgctcgt cgctccgctt cgaggcctcg gcgtggtcga cgtcggcggg
     2641 gaagatgacc ttcgcgcggt aggtcccggg cttgcggacg cccagacggg cgcggaagcg
     2701 cccgtcgctg atcgtcgccg tcgcctcccg catcacgacc cccttgcgca ggagctgcac
     2761 cgtgacggtc tggccgtcgt gcggagggtc gatcgtgccg cggacgagcg cccggtcgaa
     2821 gaggcgcaca tcggagagcg cgatcgtgag cttcggccgg acgaggacca gcaggggctc
     2881 gctctcgaag aggccccaga cggccttcgc ccgcacgttg cgtgtgagcg gtacggacac
     2941 gacgtcgtag gccccgccgc tgatcgtcgc ggccggcgcc agcgactccg tgggatcgtc
     3001 ggcgttcacg atctgcacgg tgtcgccgtc cgaggcgctc ggaaccgctc cccggaacgt
     3061 caccgtcccg ccactgggaa tgctcgcggc cggaacggcc tcgagggtga cctcccccgc
     3121 ggcgcccgcc ggcaacgccg gaaggccgac gaggaacgcg acgagcaggg ggcggaggcg
     3181 cagcatcgcc cgatgctacc gggtccggcc cacgctgtcg cgggacgtcc ctgcgacctc
     3241 gccgttcgat ccgagggcgg gcgacgtccc ccgtgcgcgg ccgttgcgcc gccgcatccg
     3301 cggccacgag gttccgacga ggatcacacc gacgccgacg atcagcgcga ccgccagcgt
     3361 ccggcttcgg atgaacgtga tgaacagcgc cgaccccgcg agcagggcgc tccacaggta
     3421 catcatcagg acagcggccc gatgcgagtg gccgatctcg agcaatcggt ggtggatgtg
     3481 ctccttgtcg gggcgtgtca cgggacggcc ccggcggatc cgccgcagga tcgcgaaggc
     3541 aacgtcggtc agcgggaccg cgagcacgag cagcgggatc gcgacgggga tcgcgagcgc
     3601 ggcgaagtcc gggttggtcg gggcgtccgt gcgccccacc ccggagatcg tcgcggcggc
     3661 gagcaggaca ccgagcagca tgcttcccga gtcgcccatg aagatcctcg cggggttgaa
     3721 gttccacggg aggaagccga tcgcgacgcc ggcgacgatg accgtcagga gcgtggcggg
     3781 tgacgggcca tcccacagcg gcgcgcccgc atcctccgcg tagacgaaga aggccaccgc
     3841 cgcgatcgtc acgacgccgg cagcgaggcc gtccaacccg tcgatcaagt tcacggcatt
     3901 caccatcacg agcacccaga tcaccgtgag tgggagcgcg aggtcgggcg agagcgagag
     3961 gatgccgagg cccggaagcc agaagtagat gaactggacc ccggcgagga tcagcacgcc
     4021 cgccgccagc acctggccgg cggccttcgc cggcgccgat atcccgcgga tgtcgtcgaa
     4081 ggcgccgagc gcgacgacga tcgtcccggc gaggacggcc ccgagcggct ctgaagacgc
     4141 ctcgaacagc gaaccgaact gcggcagcgc gtaggcgaca ccgagcccga cgaccgcccc
     4201 cgcccacagc gcgagaccac cgagggtcgg cgtcggcttc gggtgcacct ttcggtccga
     4261 cgggcggtcg atgagcccga accggaccgc gaaccggcgg acgagcggcg tcgtgacgaa
     4321 ggccgtcagg gccgacgcgc cgaagaccgc cgcgtacgcc ttcacggata ggcggggaag
     4381 cggctcgtga ggtcgcggac ccgcgcccgc ccccgctcgc gcacggcctc gtcgtcgggc
     4441 gcacggagca cctcgccgat cgtctcggcg atctccttca tctcgccctc gtccatgccg
     4501 agcgtggcgg gaccgggcgt gccgactcgg atcccggagg tcgtcatggg cgggagcggg
     4561 tcgtagggga tcgcgttctt gttgagggtg atcccgacgg cgtcgcaggc ggcctccgcg
     4621 tcggcgccgg tgacgccgat cggttggagg tcgacgagcg cgaggtgtga gtccgtgccc
     4681 ccggagacga gccgcaggcc ctggtcctga agcccggcgg cgagcgcccg ggcgctgcgg
     4741 acgacccgct cggcgtagtc gcggaagccc ggcgccatcg cctccttcag cgccaccgcc
     4801 ttcgccgcga tggcgttctc gatcggcccg ccctgcatca tcgggaagac cgtcttgtcg
     4861 atcgccttcg catgctgctc gcggcagagg atcatcgcgc cgcgcgggcc gcggagggtc
     4921 ttgtgggtcg tgaacgtgac gacgtcggcg tgcggcacgg ggctcgggta ggcgccgccg
     4981 gcgacgagtc cgatgaagtg cgcggcgtcc accatgaaga tcgcaccgac ctcgtcggcg
     5041 atctcgcgga acgcggcgaa gtcgatcgtc cgagggtagg cggtcgcgcc ggcgacgagg
     5101 accttcgggc ggtgctcccg cgcgaggtcc cggacgcggt ccatgtcgat gagctccgtg
     5161 tccttgtcga gctcgtaggg gacgacgttg aaccacttcc cggagaagtt cacgggcgag
     5221 ccgtgcgtga ggtgaccgcc gtgcgggagg ctcatgccga gcagggtgtc tccgggctgc
     5281 atgaacgcga agtacgccgc catgttcgcc tgcgcgcctg cgtgcggctg cacgttcgcg
     5341 tgctcggcgc cgaagagctc cttcgcccgc tcgatcgcga gcgactccgt cacgtcgacc
     5401 cagcggcacc caccgtagta ccggcggaag gggtagccct cggcgtactt gttgttcatc
     5461 gacgaggaga gcgcggcgag caccgcgggg ctggtgtagt tctccgacgc gatcagccgg
     5521 atcgtgctcc gctcgcgctc cagctcgccg cggatcgcat ccgcgacctc gggatcgacc
     5581 cgctcgagct cctcgacgtc ggccgcccac tccctcatgt cgtcgcctcc tcgtcttcga
     5641 tctcgcggat caggtccagt cgccgcacgt ggcggccccc ctcgaacggc gtcgagagcc
     5701 atcggtcgac gatcgcgtgc gcgagcgccg gggcaacgat ccgagcgccg atcgagagga
     5761 cgttcgcgtc gttgtgctcg cgtgcgagcg ccgccgtgaa caggtcgttg cagagcgcgg
     5821 cacgcacgcc gcgcaccttg ttcgccgcga tctgctcccc ctggcccgac cccccgagca
     5881 cgatcccgcg atcggcagcg ccgtcgcgca ccgcgcgccc gaccgccgcg cagaagggcg
     5941 ggtagtcgac cgcctcctcg gagtgggtcc cgagatcctc gacctggtgc ccctgctcct
     6001 tcaggaagcc cttgaggtcc tccttcagcg cgtagccggc gtggtcggac ccgatcgcga
     6061 tccgcacctc aggcctccgc cgtccgcgcg gcgcccgtgc ccgcgccgag ccccgcgagg
     6121 atctcgtcga ccgccacgtc gacctcgagc gcgatgtggc gatagatctc catgccgagc
     6181 ccgagcgggt ccgcgacgtc gtccacgagc gccgggtccc ggtgccggtc ggcctcggca
     6241 aggcgcgctc taacgtcccc cgtccccggc aggccccgca ggatcgccgc gagctccttg
     6301 agcgtgaacg tgcgggcggc ggccgagggg tcgagggcga ggatcccttc gacgtgctcg
     6361 gccgtcatcc cgaggatcag gtccgcctcg cggatcgccg ccgacgtcag acgcgacgac
     6421 cgatgtccct cgaggtcgac gccacgctcc cggcccgcca gccgcgactc gcgcgtcgcc
     6481 ggcatgccat cgttcgccca ggttcccgcc gaacgcacgc tgaggccgcc ggggacgagg
     6541 tcggagcagg cggcggagaa cgcctcggcg aggggggacc ggcagatgtt gccggtgcag
     6601 acggcgagga cgcgcagcac tccggggagt ctaacaaagg gggttccgcg gcccggtggg
     6661 gctcactcga cctcgccgag cacctcgcgg atcgcctcga gcggcaccgc cccgtgtcgg
     6721 atcacccgtg gcggcgcgtg cgtcaggtcg acgatcgacg aggggacctt gtgccgcgcg
     6781 ggcccggcgc agagatagat ggccacgccc tccccgagag cggcgaccag ctcctcgcac
     6841 tggtccggcg tcggctcgcc ggagcggttc gcgctcgtca ccgcgagagg cccggtccgg
     6901 gcgagcagcg agagcgtcat ctcgtggtcc ggcacgcgga tcccgacggt cccgccagcg
     6961 tcgccgaggt cccacccctc cgaagccgcg gt
//
LOCUS       DATLGG010000019        10429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_64052, whole genome shotgun sequence.
ACCESSION   DATLGG010000019 DATLGG010000000
VERSION     DATLGG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64052"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1735)
                     /locus_tag="VM638_00455"
     CDS             complement(137..1735)
                     /locus_tag="VM638_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM10930.1"
                     /translation="MTNGRIRILVLIAMAGAVVGSTPLPAASGIDDASAADCALPGID
                     DRCEKWARRYTNGTGEAPFANHTNDFTVTGAVNRAGTRAFTVGERVGGPGTVKFEMQT
                     VAYDLVTGERLWVAHEPGPSEDWHARSRAVAMSPDDRTIFVAGHVTTSGKADVLIVAY
                     DAATGQRRWRTVYDGPTGGVDLARAVAASASGELYVAASVEASDLKTISGGADLDFAL
                     LTLDASSGNITARMMYGGAPFKVDGRDRASSDVPSHIAMSPDGSQLVVAGSTFGRAAY
                     AQGRAQVSVVAWEPRDDASQGLRQRWEHELPGTAIGVAALDDGSAVLAGASSEPSSTE
                     RPHSVVRFAPDGSLRWKTRFELGRHAHFVEEPVDLLMDHERSRVYLPGLSRHPTESYG
                     TMGAVALDAAGGTVVWTAWARDARWETMRIFDDGDYFPVGALDPASGHVQLAGTREGM
                     IATVSLDRRTGAKRWVGWYDHPLREGPYLEQSAYDWPMAMRAAAGRVVVFGASGVPNR
                     QMCPFGPAFLPCSREDVLTIAYEV"
     gene            complement(1738..3252)
                     /locus_tag="VM638_00460"
     CDS             complement(1738..3252)
                     /locus_tag="VM638_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10931.1"
                     /translation="MPGLDDRCEDWVAHHTKTEGEAPFARSSHDVAIGGALSPAGDVA
                     FAFGNHYDFSSDLRRQLQTVAYDVRTGARRWVAHEPPHQPGWETQARDIVVSPDGTTV
                     YVLGAVADGSAGSEDRRDLLIAAYDATNGQRRWRTVYDGPARRYDDAHALAVSPDGQR
                     VLVAAGLGASALPVREGGPSRDLDFGLLDLDAETGEVQRTTTYAGGTFAIDGTTYASA
                     DEPRHIAVSADGKRVALAGVTAGRESYRTDRAHLTVATWERPSEDAGWRLSWARELRG
                     VALGFARVGEDFALTGAAASNATSSNPYATILLDGDDGATRWETRFEMGDRFPIAENP
                     RGLLVDEQRGRILVHGLSPNAGSADGTSGVVMLGAANGQVLWRRPLHDARNDLTLFFD
                     YDRDGSPVAGFDASTGHVLVSANKEARVITYAIDGATGTRRWTAIHDHPVRSGSYLEQ
                     SDWNFAAGVEAANGRAVIFGHAYVPRRDTCPNPNPLLPPTPCPKNDFLTIAYEV"
     gene            complement(3529..3900)
                     /locus_tag="VM638_00465"
     CDS             complement(3529..3900)
                     /locus_tag="VM638_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVM10932.1"
                     /translation="MARSEERPGADRLPPPPPGWLFLDPAAVPERWRGRAITMAMVPL
                     MPDEAAHILNGGSAAPGLEPVDEPLARLLARGTNTEDMAAILGMSMRTTQRRIAALRR
                     KIGARSKTELERMLAARGYAS"
     gene            complement(4114..7029)
                     /locus_tag="VM638_00470"
     CDS             complement(4114..7029)
                     /locus_tag="VM638_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM10933.1"
                     /translation="MEVPTARRTAGPTDAPTRASSPSLVGRDRELGLLLDTVASPPAV
                     VVIEGEPGIGKTRLVQEAVQAVDPLPTLLGRCHDLREPFPLGPVVEALRGCIAALPRD
                     AELTSNAGALHELLPELAERLPPAPPRAGTAAASRHRVFRAIRELLERIGPCLLVLED
                     LHWADRSTADLLRFLVPDMPEGLAICLTYRPDDLPSDLGIDLLRTRLPRGVLGATIGL
                     RPLSREEVGALVSAILEVDHVSPAFAGYLHERSDGLPLAVEELIRLLRDRRDIVRQGG
                     AWARRAIENIALPDALRGLVLERLGRLSREARLVVEAAAILGDAADEGLLGRVSRLSP
                     GPRVAALSEGVEAGLLEDDPPGSFDVRHSLTREAIEAVLPSPRRRELHLRAAEALEEQ
                     DPPPFARIARHFGEAGRTAELVDYAEKAAGLALERGDDATAVRVLADALRVENLDPET
                     RARLGLQLATAALHALAHVDAIPLLEAILRDDLPPDRRGELRLALGRLLVQAGRGADA
                     EIQFELAVEELRHRPDFVARALANLALPTTLEPKASHLRWAERAVEVVEATPDPATRA
                     AVVGDRAATLLYLGHRDAWRAIDDLPPPGPGLEQRRELVRVLHNLAEAALYLGHYDRA
                     RRFAERCAELAGELRYERFASHLQAIEIALAWAQGAWVGLDERATELSASAASAGPAA
                     DADLVRGLLSLVRGSAGEARDLLASVAETAELAAWLPLLTAARAGLARLALMRADPET
                     AMTEVGLVLDAIRRKGVWAWAGEMLPVAVEAQVHAGDVAAAERLVAEAALGIRGGLAV
                     ATRAAVTVSRGMIAEVRGDLDASERGFAAAEKTWQQLGRPYDATRAHAARGNVLLLSG
                     RRAGRDLLISALEDFERLGAAWDAGRLRAMLRRHGISAPRAGGRPAYGRELSPREREV
                     AELVSEGRTNREIAEALFVSTRTVEFHVANLLRKLDVTNRTALALALRSPQRAEKTP"
     gene            7152..8261
                     /locus_tag="VM638_00475"
     CDS             7152..8261
                     /locus_tag="VM638_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10934.1"
                     /translation="MRKAAIILALALPLGLAPASAGERGGKAVQGFSVDPEVLCEIRD
                     QWIDQLLAENPRGGWVPMKMEPTDQQLAMIGLPPANVLRSRSYEQPTIVAADGRTATI
                     SMAELQAAMRAQPVAAYAGTGCLGIRPGAWLLLLDQGVSWCSLAHVYGSPGSYDISTA
                     GHCGKTGTTATVIAAFGNRGGIAGPVLLDFGKFAKSQDGGLGKDWALIDIYSSAQGLV
                     SPTMCFWGGPKGMYTKTGDVAAFNWGGKGPSVSVTPDPLLAQGVAHYGHGLGVGLGGT
                     PRAGAAIAWRSTHFMFTGAISPGDSGSGSNAVVGDMTSGGPMEAAGINTHLWIDPIMR
                     SGIGIMGGTRATQVTGTLANGQLIPYPAPCAGCPG"
     gene            8358..8651
                     /gene="gatC"
                     /locus_tag="VM638_00480"
     CDS             8358..8651
                     /gene="gatC"
                     /locus_tag="VM638_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVM10935.1"
                     /translation="MPVEIDIEHVARLARLALTPEELERFRTQLPMILEHAERVGEVA
                     AQDVPPTGHPVPLTNVYREDEPRPSLAHEEALANAPETEDGRFKVPRITEAES"
     gene            8651..10123
                     /gene="gatA"
                     /locus_tag="VM638_00485"
     CDS             8651..10123
                     /gene="gatA"
                     /locus_tag="VM638_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HVM10936.1"
                     /translation="MALSDRTGHELTELLRSKEVSALEIVESCLDRGDAIDDRVQAFL
                     ERTPELAREQAGEIDVRLASGEDLPPAAGIPLALKDVFTTRGVTTTCGSQILRSYVPP
                     YDATCWARLRAAGSPLLGKTNCDEFAMGSSNENSSFFPTRNPWDLETVPGGSSGGSAA
                     AVAVGEAVWALGSDTGGSVRQPAALCGVVGMKPTYGLVSRYGLIAFASSLDTVGTFTR
                     DVRDCATLLSTIAGHDPMDSTSLPEPPRDYTGGLDQGVRGLRVGVLRDWFGEGLQPGV
                     RSAVEGAIERLAGLGAEIGEASLPNAEYALSAYYLIAPAECSSNLARYDGVRYGHRAE
                     DPRDVLDMHLRTRGEGFGQEVKRRIMLGTYSLSAGYYDAYYGQAQKVRTLVIRDFEQA
                     FASYDLLLSPTSPTVAFRIGEKADDPLAMYLSDIFTIPANLSGVPAISIPCGLGEGGL
                     PVGLQLMGRTRDEATVLRAAYALEQDLGFDARPALLTEVA"
     gene            10120..>10429
                     /locus_tag="VM638_00490"
     CDS             10120..>10429
                     /locus_tag="VM638_00490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM10937.1"
                     /translation="MSQRPVARQFGRVADIYAEAEMRRYPPDLVLGLADPDHEDHHLD
                     VGTGPGTLARMIAPHVRWSAGTDVTPEMVDLCTTRAPATACVMADAHRLPFDAATFTL
                     V"
BASE COUNT         1452 a         3723 c         3740 g         1514 t
ORIGIN      
        1 ggaaccacgc aatcgacggg ggtcggatcg agcgtgactc cggtggtccc gaggaccggc
       61 gcgatgggca tcagaacgag agcgatcgat gccatcggga tgacggatct tctcatcgag
      121 gatctccttc cacggctcat acctcgtagg cgatcgtcag cacgtcttcc cgcgagcagg
      181 gcaggaaggc ggggccgaag ggacacatct gccggttcgg cacgccggag gccccgaaca
      241 cgacgacccg gcctgccgcg gcgcgcatcg ccatcggcca gtcgtacgcg gactgttcga
      301 ggtacggacc ctcgcgcagc gggtggtcgt accagccgac ccatcgcttc gcgcccgtac
      361 ggcggtcgag cgagaccgtg gcgatcatgc cttcgcgcgt ccccgccagt tggacgtgtc
      421 cggaagccgg gtcgagggcc ccgaccggga agtagtcccc gtcgtcgaag atgcgcatcg
      481 tctcccatcg ggcgtcgcgg gcccaggccg tccagacgac cgtgccgcca gcggcgtcga
      541 gggcaaccgc tcccatcgtt ccgtagctct ccgtcgggtg acggctcagc ccggggaggt
      601 agacgcggct ccgctcgtgg tccatgagca ggtcgacggg ctcctcgacg aagtgggcgt
      661 gcctgccgag ctcgaacctc gtcttccacc ggagcgatcc atccggggcg aagcgaacca
      721 ctgagtgcgg acgctccgtg ctggacggtt cgctgctcgc cccggcgaga acggcggagc
      781 cgtcgtcgag agcggcgacg ccgatcgcgg ttccgggaag ctcgtgttcc catcgctgtc
      841 gcaggccttg tgatgcgtcg tcccgaggct cccaggccac gacgctgacc tgcgcgcggc
      901 cctgagcgta ggcggcccgc ccgaaggtgc tgcccgccac gaccaactgg ctcccgtcgg
      961 ggctcatcgc gatgtgggag ggcacgtcgg acgaggcacg gtcgcgtcca tcgaccttga
     1021 acggagcacc gccgtacatc atccgcgcgg tgatgttgcc ggagctcgcg tcgagcgtga
     1081 ggagcgcgaa atccaggtcg gctccccccg agatggtctt gaggtcgctc gcttcgacgg
     1141 atgcggcgac gtacagctct ccggaggcgg aagcggccac cgcccgggcg aggtcgacac
     1201 ctcccgtcgg gccgtcgtac acggtgcgcc aacggcgctg tcccgtcgcg gcatcgtagg
     1261 ccacgatcag gacgtcggct ttgcccgacg tggtgacgtg gcctgccacg aagatcgtcc
     1321 ggtcatccgg gctcatcgcg acggcgcgcg acctcgcgtg ccagtcctcg gagggaccgg
     1381 gctcgtgggc gacccatagg cgttcgccgg tcaccaggtc gtaagcgacc gtctgcatct
     1441 cgaacttcac cgtgccgggc ccgccgaccc gctcaccgac cgtgaaggca cgcgtgcctg
     1501 cccggttgac cgcacccgtg accgtgaagt cgttcgtgtg gttggcgaac ggggcctcgc
     1561 cggtgccgtt cgtgtacctg cgtgcccatt tctcgcagcg gtcgtcgatg cccggcaagg
     1621 cgcagtccgc cgctgatgcg tcatcgatgc cggacgctgc tggtagcggg gtcgagccga
     1681 cgacggcacc cgccatcgcg atcaggacga gtatgcggat ccttccgttg gtcatcctca
     1741 cacctcgtag gcgatcgtca ggaagtcgtt cttcgggcag ggggtcggtg gcagcaacgg
     1801 gttggggttc gggcaggtat cccggcgcgg gacgtaggcg tggccgaaga tgacggccct
     1861 cccgttggca gcttcgacgc ccgccgcgaa gttccagtcc gactgctcga ggtaggagcc
     1921 cgagcggacg ggatgatcgt ggatcgcggt ccaccggcgc gtcccggtcg ccccgtcgat
     1981 ggcgtacgtg atgacgcgcg cctccttgtt cgcggagacg aggacgtggc ccgtcgaggc
     2041 gtcgaagccg gcgaccggcg agccgtcgcg gtcgtaatcg aagaacagcg tcaggtcgtt
     2101 gcgcgcgtcg tgcagcgggc gcctccacag aacctgaccg ttcgccgccc cgagcatgac
     2161 gacccccgag gttccatccg ccgagcccgc gttcgggctg aggccgtgca cgaggatccg
     2221 gccacgctgc tcgtcgacga ggagcccccg tgggttctcc gcgatcggga accggtcgcc
     2281 catctcgaac cgtgtctccc accgcgtagc gccgtcgtcg ccgtcgagca ggatcgtcgc
     2341 gtagggattc gacgaggtgg cgttgctggc cgcagcaccg gtcagcgcga agtcctcacc
     2401 gacccgagcg aacccgaggg cgacgccccg cagctcccgt gcccaggaaa gcctccaccc
     2461 cgcgtcctcg ctgggccgct cccaggtcgc gaccgtgagg tgcgcacggt cggtccggta
     2521 cgactcgcgt ccggcggtca cccccgcgag cgcgacgcgc ttcccatcgg ccgagaccgc
     2581 gatgtgccgg ggctcgtcgg cggaggcgta ggtcgtgccg tcgatggcga aggtgccgcc
     2641 cgcgtacgtc gtggtccgct gaacctcgcc ggtctctgcg tcgaggtcca gcaggccgaa
     2701 gtccaggtcg cgtgagggac cgccctccct caccggaaga gcggaggccc cgaggcctgc
     2761 ggccaccagc acgcgctgtc catccggcga aaccgcgagc gcgtgcgcgt cgtcgtaccg
     2821 ccgggcggga ccgtcgtaga cggtgcgcca tcgacgctga ccgttcgtgg cgtcgtacgc
     2881 cgcgatcaga aggtcgcgcc gatcctctga ccctgcggac ccgtcggcga cggcgccgag
     2941 gacgtagacg gtcgttccgt ccgggctcac cacgatgtct cgcgcctggg tctcccaacc
     3001 gggctggtgc ggcggctcgt gcgcgaccca gcgccgggcg cccgttcgaa cgtcgtaggc
     3061 gacggtctgg agctgccgcc tcaggtcgga ggagaagtcg taatggttcc cgaacgcgaa
     3121 tgccacgtca ccggccggcg acagcgcacc accgatcgcg acgtcgtgac tgctccgggc
     3181 gaagggagcc tcgccctcgg tcttcgtgtg gtgcgccacc cagtcctcgc agcggtcgtc
     3241 taggccgggc acggggcagt ccgcggccac ctgtgccccg gccggtagag gcgctagcaa
     3301 gctgcccagc accgcaacga ggatcgccgc actggatcga cgaatgactg ggacgatcgc
     3361 gaacgtagct ttcctgctcg ggctcatgcc gtttccctcc ctctgatcct gccggggcga
     3421 cgagatcctt gccccccggt ggaactgccc cgggggattc gtcaggttgt gctcggtttc
     3481 ctgcctgctt cgccaagtcc tggcgagcgt ggccacgcca tccgccattc acgaggcata
     3541 tccccgtgcg gccagcatcc gttccagctc tgtcttcgat cgggcgccga tcttgcggcg
     3601 gagcgccgcg atcctgcgct gcgtcgtccg catactcatc ccgaggatcg cggccatgtc
     3661 ctccgtgttc gtgccgcggg cgaggaggcg cgccaacggc tcgtcgacgg gctccagtcc
     3721 cggcgcggcg ctccctccgt tgaggatgtg cgctgcctcg tccggcatga gcgggaccat
     3781 cgccatggtg atggctcgtc ctcgccagcg ctcagggacc gcggccgggt cgaggaacag
     3841 ccaccccggc ggcgggggcg gcagacggtc ggctccagga cgctcctcag agcgggccat
     3901 acccctcctc gaagtgcgga tcgtcgctgg gctcttcagc ggttccctcg accgggtccg
     3961 gtggttcggc gatcggggcc gatccgtacg acaggatctc cgggccctcg gatgcagggt
     4021 cgttcgctgc tcggtcgtga gcgggtcggc ggttcggtcc tggccgcatg acccgatcct
     4081 gccggggctc ggcgagggcg ccaactgtgg aacctacggg gttttctcgg cccgctgcgg
     4141 cgagcggagc gccagcgcga gggcggtgcg gttggttacg tccagcttcc gcaggaggtt
     4201 cgccacatgg aactcgacgg ttcgggtcga cacgaagagc gcctcggcga tctcccggtt
     4261 cgtcctgccc tccgagacca gctcggcgac ctcccgttcg cgaggcgaga gctcacgacc
     4321 gtaggcggga cgccctccgg cgcggggcgc ggagatgccg tggcgtcgga gcatcgcccg
     4381 gaggcggccg gcgtcccacg cggccccgag ccgttcgaag tcctccaggg cggagatcag
     4441 caggtctcgg ccggcccttc gcccgctcag cagcaggacg tttcccctgg cggcgtgagc
     4501 tcgcgtggcg tcgtacggcc ggcccagctg ctgccacgtc ttctcggccg cggcgaaccc
     4561 tcgctcgctg gcatcgagat cgccccggac ctccgcgatc atcccgcgtg agaccgtgac
     4621 ggcggcccgg gtcgcgaccg cgagaccacc gcggatcccg agcgctgcct cggcgaccag
     4681 ccgctcggcg gccgcgacgt cgcccgcgtg gacctgggct tccacggcca cgggcagcat
     4741 ctctcccgcc caagcccaca cccctttccg tcggatggca tcgagcacca ggccgacctc
     4801 ggtcatcgcg gtctccggat cggcccgcat cagcgccagc cgagcgagcc ccgctcgtgc
     4861 ggccgtgagc agcggtagcc aggcggccag ctcggccgtc tcggcgacgg aggccagcag
     4921 atcacgggcc tcgccggccg atccgcggac gagggagaga aggcctcgca ccaggtcggc
     4981 gtccgccgcc ggcccggccg aagcggccga ggcgctgagc tcggtagctc gctcgtccag
     5041 gcccacccac gcgccttggg cccaggccag cgcgatctcg atcgcctgga gatggctcgc
     5101 gaaccgctcg taccgcagct ccccggccag ctcggcgcag cgttcggcga acctgcgcgc
     5161 gcggtcgtag tgcccgaggt agagcgccgc ctcggcgagg ttgtggagca cgcgaacgag
     5221 ctctcgccgt tgctcgagcc ccggtccggg cggcggcagg tcatcgatcg cgcgccatgc
     5281 gtcccggtgt ccgaggtaca agagcgttgc ggcccggtcc ccgacgaccg ccgccctcgt
     5341 cgccggatcg ggcgttgctt cgacgacctc caccgcgcgc tccgcccagc gcaggtggga
     5401 cgctttcggc tcgagcgtcg tcggcaaggc gaggttcgcc agcgcccgcg cgacgaagtc
     5461 gggccggtgg cggagctcct cgacggccag ctcgaactgg atctcggcgt ccgccccgcg
     5521 gcccgcctgc acgagcaggc ggcccagcgc gagccggagc tccccgcgac ggtcgggcgg
     5581 gaggtcatcc cggaggatcg cctcgaggag cgggatcgcg tccacgtgtg cgagcgcgtg
     5641 aagcgccgcg gtggcgagct ggagcccgag acgcgcgcgt gtctccgggt cgaggttctc
     5701 gacccggagg gcgtcggcga gcacgcgcac ggccgtcgcg tcgtcgccac gctcgagcgc
     5761 gagccccgcg gccttctcgg cgtagtccac cagctcggcc gtgcgacccg cttcgccgaa
     5821 gtgacgcgcg atgcgtgcga acggcggagg gtcctgctcc tcgagcgcct ccgcggctcg
     5881 cagatggagc tcgcgtcgac ggggcgacgg aaggaccgcc tcgatcgcct cgcgggtgag
     5941 cgagtggcgc acgtcgaacg agccgggcgg atcgtcctcg aggagccccg cctcgacccc
     6001 ttccgacaat gccgccacgc gaggtccggg ggacagcctg gacacgcggc ccaacaggcc
     6061 ctcgtcggcg gcatccccaa ggatcgctgc agcctccacg acgaggcgcg cttcgcgcga
     6121 caggcggccg agacgctcga ggacgagccc gcgcagcgca tccgggagcg cgatgttctc
     6181 gatcgcgcgg cgggcccagg cgccgccctg acgaacgatg tcgcgacgat cgcgcaggag
     6241 ccggatcagc tcttcgaccg cgagcggcaa cccgtcgctc ctctcgtgca gatagccggc
     6301 gaacgccggg gagacgtggt cgacctccag gatcgccgag acgagcgcgc cgacctcctc
     6361 ccgcgagagc gggcggagcc cgatcgtcgc gccgaggacc cccctcggca ggcgcgtccg
     6421 caggaggtcg atgccgagat cggagggaag gtcgtcggga cggtacgtga ggcagatcgc
     6481 gagcccctca ggcatgtccg ggaccaggaa gcggagcagg tctgcggtcg accggtccgc
     6541 ccagtggaga tcctcgagca ccaggaggca gggtccgatc cgctcgagca gctccctgat
     6601 cgcccggaag acgcgatgcc ggctcgctgc ggcggtgccg gctcgcggcg gcgccggagg
     6661 aagccgctct gcgagctcgg ggaggagctc gtggagggcg ccggcgttgc tggtgagctc
     6721 cgcgtcccgc ggcagcgccg cgatgcaccc ccgcagggcc tccaccaccg gcccgagggg
     6781 gaagggctcc cggaggtcgt ggcagcggcc cagcagcgtc gggagcgggt cgaccgcctg
     6841 caccgcttcc tggacgaggc gggtcttccc gatcccgggc tctccctcga tcacgacgac
     6901 cgccgggggg gacgcgacgg tgtcgagcag gagcccgagc tcccggtcac ggccgacgag
     6961 cgacggtgac gaggcccgcg tcggcgcgtc cgtcgggccg gctgttcggc gtgcggtcgg
     7021 gacctccacg gctgacaggg ttcgccaccg gaggcgggca ttcctgccgg gcgggggaga
     7081 gcgatgtgcc tgattcgtcc cggttaccgc ggggtaggtt tcctggtgga cccgcacgga
     7141 aggaggctcg catgaggaag gcagcgatca tcctggccct ggcgctcccg ctggggctcg
     7201 caccggcgtc cgccggtgag cgcggcggga aggccgtcca gggcttctcc gtcgacccgg
     7261 aggtgttgtg tgagatccga gaccagtgga tcgaccagct gctcgccgag aaccctcggg
     7321 gcggctgggt cccgatgaag atggagccca ccgaccaaca gcttgcgatg atcgggctcc
     7381 cgccggcgaa cgtcctgagg tctcgttcgt acgagcagcc gacgatcgtg gcggccgacg
     7441 gccggaccgc gacgatctcg atggccgagc tccaggccgc gatgcgcgcg cagcccgtcg
     7501 ccgcctacgc ggggaccggc tgcctgggca tccggcccgg cgcctggctc ctgctcctcg
     7561 accagggcgt gagctggtgc agcctcgccc acgtctacgg ctcgcccggg agctacgaca
     7621 tcagcaccgc cggccactgc ggcaagaccg gcacgaccgc gacggtgatc gcggccttcg
     7681 gcaatcgcgg cggcatcgcc ggaccggtgc tcctcgactt cgggaagttc gcgaagtcgc
     7741 aggacggcgg gctcgggaag gactgggcgc tgatcgacat ctacagctcg gcgcagggtc
     7801 tcgtgagccc gacgatgtgc ttctggggcg gcccgaaggg gatgtacacg aagaccggtg
     7861 acgtcgccgc gttcaactgg ggcggcaagg gcccgagcgt gagcgtcacg cccgatccgc
     7921 tcctcgccca gggcgtggcc cactacgggc acggcctcgg tgtcggcctc ggtgggacgc
     7981 cgcgagccgg cgcggcgatc gcctggcggt cgacgcactt catgttcacg ggcgcgatct
     8041 ccccgggtga ctccggctcc ggcagcaatg cggtggtcgg cgacatgacc agcgggggcc
     8101 cgatggaggc cgccgggatc aacacccacc tgtggatcga cccgatcatg cgcagtggga
     8161 tcgggatcat gggcggcacg cgcgcgaccc aggtgacggg gacgctggcc aacggccagc
     8221 tgatcccgta cccggcgccg tgcgccggct gccccggtta gcacgagcgt gacggcaggc
     8281 tcccggctcg accgggggcc tgccgtcccc acgcctgacc cgtcgggccc cggtgggcgg
     8341 ttgctagact cgccggcgtg ccggtcgaga tcgacatcga acacgtcgcg cgtctcgcgc
     8401 ggctcgcgct gacccctgag gagctcgagc gcttccgcac gcagctcccg atgatcctcg
     8461 agcacgcgga acgtgtgggg gaggtcgccg cgcaggacgt tcctccgacc ggccatccgg
     8521 tgccgctgac gaacgtctac cgcgaggatg agccgcggcc gtcgctcgcg cacgaggagg
     8581 cgctcgcgaa cgcgcccgag acggaggacg gccgcttcaa ggtgccgcgc atcaccgagg
     8641 cggagtcgta gtggcgctgt ccgaccggac gggccacgag ctcaccgagc tcctgcgctc
     8701 gaaggaggtc tccgcgctcg agatcgtcga gtcgtgcctc gaccgcggcg acgcgatcga
     8761 cgaccgggtc caggcgttcc tcgagcgaac gccggagctc gcgcgcgagc aggcggggga
     8821 gatcgatgtt cgactggcct cgggcgagga tctcccaccg gccgcgggga tccccctggc
     8881 gctgaaggac gtcttcacga cgcgtggcgt caccacgacg tgcggctcgc agatcctccg
     8941 ctcctacgtg cccccgtacg acgcgacctg ctgggcacgg ctgcgcgctg cgggctcgcc
     9001 gctgctcggc aagacgaact gcgacgagtt cgcgatgggc tcgtccaacg agaactcctc
     9061 gttcttcccg acacggaacc cgtgggatct cgagaccgtg ccgggcgggt cctccggcgg
     9121 atcggcggcg gcggtggcgg tgggcgaggc ggtctgggcg ctcggaagcg acaccggtgg
     9181 ctccgtccgt cagccggcgg cgctgtgtgg tgtggtcggg atgaagccga cgtacggcct
     9241 cgtgtcccgc tacgggctga tcgcgttcgc atcctctctg gacaccgtcg gcaccttcac
     9301 gcgggacgtc cgggactgcg ccacgctgct ctcgacgatc gccgggcacg acccgatgga
     9361 ctcgacctcg ctcccggagc ccccgcggga ctacaccggg gggttggacc agggtgttcg
     9421 cgggctgcgg gtcggcgtcc tccgcgactg gttcggtgag gggctccagc cgggcgttcg
     9481 gtcggccgtg gaaggcgcga tcgagcgcct cgccggactc ggtgccgaga tcggcgaggc
     9541 gtccttgccg aacgcggagt acgcgctctc ggcttactac ctgatcgcgc cggcggagtg
     9601 ctcctcgaac ctcgcgcgct acgacggcgt ccgctacggc catcgggccg aggatccgcg
     9661 cgacgtcctc gacatgcacc tgcggacgcg cggcgagggc ttcggccagg aggtcaagcg
     9721 ccgcatcatg cttggcacgt actccttgtc ggcggggtac tacgacgcct actacggaca
     9781 ggcacagaag gtccggacac tcgtgatccg cgacttcgaa caggccttcg cttcgtacga
     9841 cctcctgctc tcgccgacct cgccgacggt cgcgttccgg atcggcgaga aagccgacga
     9901 cccgctggcg atgtacctct ccgacatctt cacgatcccg gcgaacctct cgggtgtccc
     9961 cgcgatctcg atcccgtgcg ggctcggcga gggcggcctt cccgtcggtc tccagctcat
    10021 ggggcggacc cgggacgaag cgacggtcct ccgcgcggcc tacgcactcg agcaggacct
    10081 cggtttcgac gcgcgaccag cgctcctgac ggaggtggca tgagccagcg gccggtcgcc
    10141 cgtcagttcg gccgcgtcgc cgacatctac gccgaggccg agatgcgccg ctacccgccc
    10201 gacctcgtgc tcgggctcgc ggatcccgac cacgaggacc atcacctcga tgtcggcacc
    10261 ggcccgggca cgctcgcccg gatgatcgcg ccgcacgtgc gctggagcgc cgggaccgac
    10321 gtcacacccg agatggtgga tctctgcacg acgcgcgcgc cggcgaccgc gtgcgtgatg
    10381 gccgacgccc accgactgcc gttcgatgcc gcgaccttca cgctcgtca
//
LOCUS       DATLGG010000020         3162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_190600, whole genome shotgun sequence.
ACCESSION   DATLGG010000020 DATLGG010000000
VERSION     DATLGG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190600"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VM638_00495"
     CDS             <1..1031
                     /locus_tag="VM638_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HVM10938.1"
                     /translation="IITVLVLTGFREAVLNAIPMAMKKAIGIGIGLFIALIGLVNAGI
                     VVHPEAGQPILHIASDLTTLRILTFVVGLVLTSALVARRARGALLIGILGTTVFAIAI
                     NYLFGGQGIWEGLPGVGLLPTAIAQSPDFSLVGQFSFDVFRVVGVATALALILSVMLS
                     DFFDTMGTVIGLGDQAGLLDEDGRLPGIRRVLLVDSLGAVAGGAASSSSNTTYIESAA
                     GVAEGGRTGLTAVVVGILFLLAMFLSPLAGVIPPEATAPVLVIVGYFMMQHVRDIEWA
                     DPGIGIPALLTVILMPFTFSITNGVAGGFLAYTVIALLRGRWRDVHPLMYLVSAVFIW
                     YFAGGYVA"
     gene            complement(1089..1346)
                     /locus_tag="VM638_00500"
     CDS             complement(1089..1346)
                     /locus_tag="VM638_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10939.1"
                     /translation="MAQTLDGATDRIVSRVDSVKLYLASPEGQRLRKALATGLIVSAP
                     IITRLPAFRRSMVGRLIGLAGGAAVIAAAARWLRDWEPELV"
     gene            complement(1346..1615)
                     /locus_tag="VM638_00505"
     CDS             complement(1346..1615)
                     /locus_tag="VM638_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVM10940.1"
                     /translation="MEIVWWLLIGLVAGALARLLVPGRDRMSWGGTLLLGLIGSVVGG
                     FLANVLLESDGEFSPAGLLGSIIGAILALLVWRAVAGRGARRGAF"
     gene            complement(1672..>3162)
                     /gene="acnA"
                     /locus_tag="VM638_00510"
     CDS             complement(1672..>3162)
                     /gene="acnA"
                     /locus_tag="VM638_00510"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HVM10941.1"
                     /translation="GGEPPAGDEPPFRSAPVELDGATEELRDGAVVIAAITSCTNTSN
                     PSVMVGAGLLARAAVERGLSVKPFVKTSMAPGSPVVMDYLRNAGLLQPLERLGFGLVG
                     FGCTTCIGNSGPLPEPIARAIDEHAISAAAVLSGNRNFEGRIHPQVRMSFLASPPLCV
                     AYALAGTVDIDLTTEPLGLDTEGQPVYLRDIWPSSEEVAEVVAGALDPEAYRERYAQI
                     FDGDERWRSLPIPEGELYEWDPDSTYITEPPFVAHIGPEPEPPKALRGARVLVSVGGS
                     VTTDHISPAGTIPPDSPAGRWLQERGVERRRLHSYGARRGHHEVMVRGTFGNIRLRNL
                     LAPGTEGGVTIHLPSGEQTSIYEAAMRYREEDTPLLVLAGTEYGAGSSRDWAAKGTAL
                     LGVRAVIAESYERIHRSNLAQMGVLPLQFLDGESRESLGLTGRETFDLEVGDLAPGMT
                     IPIVARRDDGTEIRFEVRCRLDTQTEVRYVQEGGILPAVVRELARR"
BASE COUNT          445 a         1124 c         1099 g          494 t
ORIGIN      
        1 cgatcatcac ggtcctcgtc ctcaccggat tccgtgaggc cgtcctcaac gcgatcccca
       61 tggcgatgaa gaaggcgatc gggatcggga tcggcctgtt catcgcgttg atcgggctgg
      121 tgaacgccgg catcgtcgtg catccggaag ccgggcagcc gatcctgcac atcgcgagcg
      181 acctgacgac cctgcggatc ctcaccttcg tggtcggtct cgtcttgacg tcggcgctgg
      241 tggctcggcg agcacgaggg gcgctgctga tcggcatcct cggcacgacg gtattcgcca
      301 tcgcgatcaa ctacctcttc ggcggtcagg gcatctggga gggcctgccg ggggtcggac
      361 tcctgccgac ggcgatcgcg caatcgccgg acttctcgct cgtggggcag ttctcgttcg
      421 acgtcttccg cgtggtcggc gtcgcgacgg ctctcgccct gatcctctcg gtgatgctct
      481 ccgatttctt cgacacgatg ggcacggtca tcggcttggg ggaccaggcg gggctgctcg
      541 atgaagacgg tcggctgccg gggatccgga gggtcctgct ggtggactcg ctgggtgcgg
      601 tcgcgggcgg ggccgcgtcg tcctcgtcga acaccaccta tatcgagagt gccgcaggcg
      661 tggccgaagg cggccggacg ggcctgaccg ccgtggtcgt cgggatcctc ttcctgctgg
      721 cgatgttcct atccccgttg gcgggcgtga tccctccgga ggcgacggcg cccgtcctgg
      781 tcatcgtcgg ctatttcatg atgcagcacg tgcgcgacat cgagtgggcg gacccaggca
      841 tcgggatccc tgcgctgctg acggtgatcc tcatgccgtt caccttcagc atcacgaacg
      901 gcgtcgccgg cggcttcctc gcctacacgg tgatcgcgtt gctgcgggga cgctggcggg
      961 acgtacaccc gctcatgtac ctggtctcgg ctgtgttcat ctggtacttc gccgggggtt
     1021 atgtggcctg agggggtccg tacgccggaa gggggcgcct gcgggcgccc ccttccgtgc
     1081 tcgtcctcct agaccagctc cggctcccag tcccgcagcc atcgcgcggc cgccgcgatc
     1141 acggccgctc cccccgcgag gccgatcaga cgcccgacca tcgagcgccg gaacgccggg
     1201 agccgggtga tgatcggcgc ggagacgatg agcccggtcg ccagcgcctt ccgcagccgc
     1261 tgtccctccg gcgaggcgag gtagagcttc acgctgtcga cgcgcgagac gatgcgatcc
     1321 gtcgcgccgt cgagggtctg tgccatcaga acgcccccct gcgggccccg cgccccgcca
     1381 cggcgcgcca cacgagcaac gcgaggatcg ctccgatgat cgagcccagg aggccggccg
     1441 ggctgaactc gccgtcgctc tccagcagca cgttcgccag gaaccctccg accaccgagc
     1501 cgatcaggcc gagcagcagc gtcccgcccc agctcatgcg gtcacggccg ggcaccagca
     1561 gtcgcgcgag cgcacccgcg accaggccga tgagcaacca ccagacgatc tccatggaac
     1621 ctcctccgct cggtatgcgg tgaccttgcc cagcgctccc agccaggaaa cctagcggcg
     1681 cgcgagctca cgcacgaccg caggcaggat cccgccctcc tgcacgtacc ggacctcggt
     1741 ctgggtgtcg aggcggcacc ggacctcgaa gcggatctcg gtcccgtcgt cgcgacgggc
     1801 cacgatcggg atcgtcatgc ccggagcgag gtcaccgacc tcgaggtcga aggtctcccg
     1861 tccggtcagg ccgagcgact cgcgcgactc cccatcgagg aactgcaggg gcaggacgcc
     1921 catctgcgcg aggttgctgc ggtggatccg ctcgtaggac tccgcgatca ccgcgcggac
     1981 ccccaggagt gccgtgccct tcgccgccca gtcgcggctc gacccggcgc cgtactccgt
     2041 tccggcgagc acgagcaggg gcgtgtcctc ctctcggtag cgcatcgccg cctcgtagat
     2101 cgatgtctgc tcccccgacg ggaggtggat cgtcaccccg ccctcggttc cgggggccag
     2161 caggttccgc aggcggatgt tgccgaaggt tccgcggacc atgacctcgt ggtgcccgcg
     2221 ccgggccccg tacgagtgaa ggcggcggcg ctcgacgccg cgctcctgga gccagcgtcc
     2281 ggcgggtgag tccggtggga tcgtgccggc gggcgagatg tggtcggtcg tgacggaccc
     2341 gccgaccgag acgaggaccc gtgcgccccg caacgccttc ggcggctcgg gctcgggccc
     2401 gatgtgcgcc acgaacggag gctcggtgat gtaggtcgag tcgggatccc actcgtacag
     2461 ctcgccctcc gggatcggga gcgagcgcca gcgctcgtcc ccgtcgaaga tctgcgcgta
     2521 acgttcccgg tatgcctccg ggtcgagcgc gccggcgacg acctcggcga cctcctccga
     2581 gctcggccag atgtcgcgga ggtagaccgg ctgcccctcc gtgtcgagcc cgagcggctc
     2641 agtcgtcagg tcgatgtcga ccgtcccggc gagcgcgtag gcgacgcaga gcggcggcga
     2701 ggcgaggaac gacatccgga cctgcgggtg gatccggccc tcgaagttcc ggttgcccga
     2761 gagcacggcg gcagccgaga tcgcgtgctc gtcgatcgcg cgcgcgatcg gttcgggcag
     2821 gggcccggag ttaccgatgc aggtcgtgca gccgaacccg acgagaccga agcccaggcg
     2881 ctccaacggc tggagcagcc ccgcgttgcg gaggtagtcc atgacgaccg gcgaccccgg
     2941 cgccatgctc gtcttcacga acggcttgac cgacagtccg cgctcgacgg cggcgcgcgc
     3001 gagcagcccc gcccccacca tcaccgacgg gttcgaggtg ttggtgcagg aggtgatcgc
     3061 cgcgatcacg accgcgccgt cgcgaagctc ctcggtcgcg ccgtcgagct cgacgggagc
     3121 gctgcggaac ggcggctcat cgcctgcggg aggctcgccc cc
//
LOCUS       DATLGG010000021         5589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_80597, whole genome shotgun sequence.
ACCESSION   DATLGG010000021 DATLGG010000000
VERSION     DATLGG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80597"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VM638_00515"
     CDS             <1..573
                     /locus_tag="VM638_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVM10942.1"
                     /translation="LVASWAGFAISRLPRRAAATVVVASLLALMIPATALLVSRFALF
                     RTLQLTDTFVPLVAPALIGTSPFYVLLYAWSFRRLPTELLEAARLEGLTPFQTWRRVA
                     MPLVRPVTVAVGVLAFVVTWSNFLDPLIYLFDEQRFTLPLGLRALARLEPRNFPLFLA
                     GAVTATAPVVAAFLVVQRFFLGREARWLRS"
     gene            558..1844
                     /locus_tag="VM638_00520"
     CDS             558..1844
                     /locus_tag="VM638_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018797082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVM10943.1"
                     /translation="MAPKLRRTAVALVAALVTVACGGTGEVADGDAVRFLVFGDPEEL
                     RGYRDAIAAFHEREPEVRVQLVEASDRDDLIARLSTSIAGGSPPDLFLINYRFFGQFA
                     AKDALAPAGPRLDASGSLDEEDFYEEPMEAFRWENELMCLPQNVSSLVVYFNRDMFEA
                     GGVPLPEPGWTWEEFVDAAAALTVDDNGDGTAEVHGVGIEPSIIRVAPFVWSNGGDLV
                     DDEERPTRLALDSPEAKEALQRLFDLGRIGVMPSEEEREAEDEETRFLNGRLGMVLSS
                     RRSTPNFRSITAFEWDVAPLPVMGEPAGILHSDAYCMTAASGRKDDAWRFVEFALGAE
                     GQRIVAASGRTVPSLREVAESEAFLDPSVPPASSRVFLDTIPYIRRVPNISTWPEIED
                     AAEGIIELGLLEGHSVDRVVSQLIEVTRPMFARAER"
     gene            1848..2909
                     /locus_tag="VM638_00525"
     CDS             1848..2909
                     /locus_tag="VM638_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM10944.1"
                     /translation="MAGLAFRDVHKDYGVAHALRGVDLEVAEGELLVIVGPSGSGKST
                     ALRVAAGLEPVSSGRVLIGGRDVTDLPPAARNVAMVFQSYALFPHLDVSENIGFGLRA
                     RRVPREEARRQVMEAAELTGCADLLDRRPDQLSGGERQRVALARALAHEPDVFLLDEP
                     LSSLDLQLRVETRAEIKALHQRLGSTMLYVTHDQLEALSIGDRVAVLHAGEVQQVGTP
                     EEVYRRPTNRFVGEFLGSPRMSILEGQVEGEVLRAGPFEVPLPGAAAALTGRAVEVGI
                     RPEHFQVDEGGPRLEVQVVESAGSDVYVHLRAGDRAVVARLASGIRPSVGSRLPLSIV
                     DGGVHLFDPKTGEALWPRP"
     gene            2936..3784
                     /locus_tag="VM638_00530"
     CDS             2936..3784
                     /locus_tag="VM638_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVM10945.1"
                     /translation="MLLPYLVGLVALVAAPAAVTFALSLFDYDLVRPARWAGGANFAE
                     LARDGVFHISLRNSLMFALFAVPLRLLGALGLGLLLHRRFRGVGAYRTAAYLPTVVPD
                     VAYALLWLWILNPLYGPLNLFLEGVGLPSPQWLTKPAAAQAAVVFMSLFQIGEGFLVA
                     MAVRQMIPGDLYEMAAVEGARPWHVLRRVTLPMMAPALLLLLARDTIYSFQYNFVPAL
                     VVTGGGPPPYATTYLPLFVYRTGFEYLRYGYAAAATLVMFAVTALIVLIQWQIVKRWR
                     RAFTTI"
     gene            complement(3978..4979)
                     /locus_tag="VM638_00535"
     CDS             complement(3978..4979)
                     /locus_tag="VM638_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVM10946.1"
                     /translation="MSRALSRDLVAPDPALERAVRSRLDEVEDALHRAVRSETRLVAE
                     AGRYLIDAGGKRFRPMLALLSGHFGNPEDPRLITGAVAVELTHLATLYHDDVIDEARF
                     RRGIPSVNARWGNTVAILTGDFLFAKSSEVASELGREVTKLLAVTIGKVAEGQIGEVE
                     VAGRVDIDEATYRHVIGRKTASLIATSCQVGGILSGADPDVQGSLARFGEGLGMAFQL
                     SDDIMDLISDEETLGKEPGVDLKEGVYTLPVILALKSDDGDELRDLLGDEELEGDRFH
                     RALEIVRSERTLDEAREAVADEVRGALAEAARLPDGSPREALEHLARFLATRCGAGV"
     gene            complement(4984..>5589)
                     /locus_tag="VM638_00540"
     CDS             complement(4984..>5589)
                     /locus_tag="VM638_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVM10947.1"
                     /translation="ALSMVIGSTLAIAQTEIKRMLAYSSISHAGFVLVGVVAANQQGI
                     SGAMFYLAAYAAMVIGAFGVVLLVSRRGEQATSLGSYAGLARRSPLLAALLTLFLLSL
                     AGLPPAAGFMGKLLVFRAAVDAGDWMLVVVAVLASVVAAFFYLRVIVLMYMQEPEEQT
                     ALEPAAGPGVALAVAAIVTLVLGVFPGVLLDVLDAASVLRW"
BASE COUNT          749 a         1959 c         2039 g          842 t
ORIGIN      
        1 ctggtggcct cctgggcggg gttcgcgatc tcccgtctgc cccggcgagc cgcggcaacg
       61 gtcgtcgtcg cctcgctcct cgcgctgatg atcccggcga ccgccctgct cgtgagccgg
      121 ttcgcgctct tccggaccct ccagctgacc gacaccttcg tcccgctggt cgcccccgcc
      181 ctgatcggga cctcgccgtt ctacgtcctg ctgtacgcgt ggagcttccg ccggctcccc
      241 accgagctgc tcgaggcggc acggctcgag gggctcacgc cgttccagac gtggcggcgc
      301 gtggcgatgc cgctcgtccg cccggtgacg gtggcggtgg gcgtgctggc gttcgtcgtc
      361 acctggagca acttcctgga cccgctgatc tacctgttcg acgagcaacg cttcacgctc
      421 cccctcggcc tgcgcgcgct ggcgcggctc gagccgcgga acttcccgct gttcctcgcg
      481 ggcgcggtga ccgcgacggc gccggtggtg gcggcgttcc tcgtcgtgca gcggttcttc
      541 ctcggacggg aggcgcggtg gctccgaagc tgaggcggac cgcggtcgca ctcgtcgctg
      601 cgctcgtgac cgtggcgtgc ggcggcacgg gcgaggtcgc cgatggagac gcggtgcgct
      661 tcctcgtctt cggcgacccg gaggagcttc gcgggtaccg cgacgcgatc gcggccttcc
      721 acgagcggga accggaggtg cgggtgcagc tcgtcgaggc gagcgaccgc gacgacctga
      781 tcgctcggct gtcgacgtcg atcgccggcg ggtcgccgcc ggacctgttc ctgatcaact
      841 atcggttctt cggtcagttc gccgcgaagg acgcgctcgc gccggccggg ccgcggctgg
      901 acgcgtcggg ttcgctcgac gaggaggact tctacgagga gccgatggag gcgttccggt
      961 gggagaacga gctgatgtgc ctcccgcaga acgtctcgag cctcgtcgtc tacttcaacc
     1021 gcgacatgtt cgaggcgggc ggggtgccgc tgcccgagcc gggctggacg tgggaggagt
     1081 tcgtcgacgc cgccgctgcg ctgaccgtcg acgacaacgg cgatgggacg gcggaggtgc
     1141 acggcgtcgg gatcgagccc tcgatcatcc gcgtggcccc cttcgtctgg tcgaacggcg
     1201 gcgacctggt cgacgacgag gagcggccga cccggctcgc gctcgactcg cccgaggcga
     1261 aggaggcgct ccagcggctg ttcgacctcg ggcgcatcgg cgtgatgccg agcgaggagg
     1321 agcgcgaggc cgaggacgag gagacgcgct tcctcaacgg ccggctgggc atggtgcttt
     1381 cctcgcgccg ctcgaccccg aacttccgct cgatcaccgc cttcgagtgg gacgtcgcgc
     1441 cgctgccggt gatgggcgaa cccgcgggga tcctccactc cgacgcgtac tgcatgacgg
     1501 cggcctccgg gcggaaggac gacgcctggc ggttcgtcga gttcgcgctc ggcgcggagg
     1561 gacagcggat cgtcgcggcg agcggacgga cggtcccatc gctgcgggag gtcgcggagt
     1621 cggaggcctt cctcgacccg agcgtgccgc ccgcgagctc acgggtcttc ctcgacacga
     1681 tcccctacat ccggcgggtg ccgaacatct cgacctggcc cgagatcgag gacgcggctg
     1741 aggggatcat cgagctcggc ctgctcgagg ggcactcggt cgaccgggtc gtctcgcagc
     1801 tgatcgaggt gacgaggccg atgttcgcgc gcgccgagcg gtagccggtg gcggggctcg
     1861 cgttccggga cgttcacaag gactacgggg tcgcgcacgc cctccgcggg gtggacctcg
     1921 aggtcgccga gggtgagctg ctcgtgatcg tcgggccgtc ggggagcggg aagagcacgg
     1981 ccttgcgcgt ggcggctgga ctggagccgg tgagctccgg gcgggtgctg atcgggggac
     2041 gggacgtgac cgacctgccc ccggccgcgc ggaacgtcgc gatggtcttc cagtcctacg
     2101 cgctcttccc ccacctcgac gtgagcgaga acatcggctt cggcctgcgg gcgaggcgcg
     2161 tgccgcggga ggaggcgcgg cgacaggtga tggaggcggc ggagctgacg ggatgcgccg
     2221 acctgctgga tcgccggccc gaccagctct ccggggggga gcggcagcgg gtggcgctcg
     2281 cccgcgcgct cgcccacgag ccggacgtgt tcctgctcga cgagccgctc tccagcctcg
     2341 accttcagct cagggtcgaa acgcgagcgg agatcaaggc cctgcaccag cggctgggga
     2401 gcacgatgct ctacgtcacc cacgaccagc tggaggcgct ctcgatcggc gaccgcgtcg
     2461 cggtgctgca cgccggcgag gtccagcagg tcgggacccc ggaggaggtg tatcgccgcc
     2521 cgacgaaccg cttcgtcggg gaatttctcg gcagccccag gatgagcatc cttgaaggtc
     2581 aggtggaggg tgaggtcctg cgcgcgggtc cattcgaggt gcccttgccc ggggcggccg
     2641 ccgccctgac cggccgcgcc gtcgaggtgg ggatccggcc cgagcacttc caggtggacg
     2701 agggggggcc ccggctcgag gtgcaggtgg tcgagagcgc cgggagcgac gtctacgtcc
     2761 acctgcgggc cggggatcgg gcggtggtgg cccggttggc ctcggggatc cggccttcgg
     2821 tcgggtcgag gctcccgctg tccatcgtgg acgggggcgt ccacctgttc gacccgaaga
     2881 ccggggaggc gctgtggccg cgaccctgac gtccgagcgc cggcggctgt ggatgctgct
     2941 cctcccctac ctcgtgggac tcgtcgcgct cgtcgcggcc ccggcggccg tcacgttcgc
     3001 gctgtcgctc ttcgactacg acctggttcg cccggctcgt tgggccggcg gagcgaactt
     3061 cgccgagctc gcgcgggacg gcgtgttcca catctcgctc cggaactcgc tgatgttcgc
     3121 gctcttcgcg gtcccgctgc ggctgctggg cgcgctcggg ctcgggctgc tcctgcaccg
     3181 gcgcttccgc ggggtcggcg cgtacaggac cgcggcgtac ctgccgacgg tcgttcccga
     3241 cgtcgcctac gcgctcctgt ggctgtggat cctgaacccg ctgtacgggc cgctgaacct
     3301 gttcctcgag ggggtgggcc tgccgagccc gcagtggctg acgaaaccgg ccgcggccca
     3361 ggcggcggtg gtgttcatga gcctgttcca gatcggcgag ggcttcctcg tggcgatggc
     3421 ggtacggcag atgatccccg gtgacctcta cgagatggcg gcggtcgagg gggcgcgccc
     3481 gtggcacgtc ttgcggaggg tgacgctgcc catgatggcg ccggcgctcc tgctgctgct
     3541 ggcgcgcgac acgatataca gcttccagta caacttcgtc cccgcgctcg tggttaccgg
     3601 cggtggcccg ccgccgtacg caaccacgta cctgccgctc ttcgtctacc gcacgggctt
     3661 cgagtacctg cgctacggct acgccgccgc cgccacgctc gtgatgttcg ccgtcacggc
     3721 cctgatcgtc ctgatccagt ggcagatcgt gaaacgctgg cgccgcgcct tcaccacgat
     3781 ctgatcgggc tcacggatgc ttcggcccgc gacgacgcgg cgtcactgca gccgacgaac
     3841 cagcggcgag atcgccgacg ggccgcttag gcttcgccgg cctgccgccg ggctccgaga
     3901 gcgagggcga tgtcggtgag ctgctgccgc atccccttca gggcgtacca gcctccagcc
     3961 gatccttcca tccggcgcta caccccggcg ccgcagcggg tggcgaggaa cctcgccaga
     4021 tgctcgaggg cctcgcgggg ggagccatca gggaggcggg ccgcttcggc cagggctcca
     4081 cgaacctcat cggccaccgc ctcgcgcgcc tcgtcgaggg ttcgctccga gcggacgatc
     4141 tccagggcgc ggtggaaccg gtcgccctcc agctcctcat ccccgagcag gtcccggagc
     4201 tcgtcgccgt cgtcggactt cagcgcgagg atcacgggca gggtgtagac cccctccttc
     4261 aggtcgaccc cgggctcctt ccccagcgtc tcctcgtcgg agatcagatc catgatgtcg
     4321 tcggagagct ggaacgccat acccagcccc tcgccgaacc tcgcgagcga tccctgcacg
     4381 tcggggtcgg cgccggagag gatcccgccg acctggcacg aggtcgcgat gagcgacgcc
     4441 gtcttccgtc cgatcacgtg ccgataggtc gcctcgtcga tgtccacgcg gccagcgacc
     4501 tccacctccc cgatctgccc ctcggcgacc ttgccgatcg tcaccgcgag gagcttcgtg
     4561 acctcacgtc cgagctccga ggcgacctcc gacgacttcg cgaacaggaa atcgcccgtg
     4621 aggatcgcga ccgtgttccc ccaccgcgcg ttgacggagg ggatgccgcg acggaagcgc
     4681 gcctcgtcga tcacgtcgtc gtggtaaagc gtggccaggt gcgtgagctc gacggcgacc
     4741 gcccccgtga tcagccgcgg gtcctccggg ttgccgaagt ggccgctgag gagcgcgagc
     4801 atcggacgga agcgcttccc cccggcgtcg atcaggtagc gcccggcctc ggcgacgagg
     4861 cgcgtctcgc tccgaaccgc ccggtggagc gcatcctcga cctcgtcgag ccgggagcgg
     4921 accgctcgct cgagcgcggg gtccggagcg acgaggtcgc ggctgagcgc gcggctcacg
     4981 ccgtcaccac cggaggaccg acgcggcgtc gaggacgtcg aggagcactc ccgggaagac
     5041 gccgaggacg agcgtgacga tcgccgccac cgcgagggcg acgccgggcc cggcggccgg
     5101 ctcgagcgcc gtctgctcct ccggctcctg catgtacatc aggacgatga cgcgcaggta
     5161 gaagaaggcg gcgacgacgc tcgccaggac ggcgacgacc acgagcatcc agtcgccggc
     5221 gtcgacggca gcgcggaaga cgaggagctt gcccatgaag ccggcggccg gagggagacc
     5281 ggccagcgag agcaggaaga gcgtcagcag cgcggcgagg agcgggctcc ggcgggcgag
     5341 cccggcgtac gaaccgagcg aggtcgcctg ctccccgcga cgcgacacga gcaggacgac
     5401 gccgaacgcg ccgatcacca tcgcggcgta cgcggcgagg tagaacatcg cccccgagat
     5461 cccctgctgg ttcgccgcga cgacacccac caggacgaac cccgcgtggg agatcgacga
     5521 gtacgcgagc atccgcttga tctcggtctg ggcgatcgcg agcgtcgagc cgatcaccat
     5581 gctgagcgc
//
LOCUS       DATLGG010000022         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_175155, whole genome shotgun sequence.
ACCESSION   DATLGG010000022 DATLGG010000000
VERSION     DATLGG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175155"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="dnaN"
                     /locus_tag="VM638_00545"
     CDS             <1..713
                     /gene="dnaN"
                     /locus_tag="VM638_00545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVM10948.1"
                     /translation="FAQAVGQVVKAASRDEARPILTGVLLEANREGLTMVATDSYRLA
                     VRELSATGEGEAKALVPERAISEAGRAAATEERAEVRMVLGDSQASFRVGGVTMTSRL
                     IDGEFPNYRQLLPEPGGNRLTAPRQELLEAVRRVGLLARENSPVRLELNALGVRLTSS
                     SPDLGGAVEVLEASYEGDELTAAFNPTYLGDGLAGVPADRVSLELRDGLKPALLRGEG
                     DDAFTYLVMPVRLPAPVG"
     gene            732..1808
                     /locus_tag="VM638_00550"
     CDS             732..1808
                     /locus_tag="VM638_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HVM10949.1"
                     /translation="MRLERLELRDFRNHRETRLEVPGGLVAAVGPNAQGKTNLLEGAY
                     YLMTLGSPRVATDQPLVRAGATSAYLRGEIRTASGRALVEVEVRSSGANRVRLNGSGI
                     RRKRDLRQRVRAVMSVPEDLAVVQGDPEERRRFLDEAAETLWPATEAARRAFDRALRQ
                     RNRLLKEHEPGFPPAELGVWDRELATHGAAVTRNRARAVDRLAPAASEAYREVAGHPL
                     RVAYRPSVEGEDLEASFLERLAERRGDELIRRRTLVGPHRDELELGVGELTARRFASH
                     GESSAAAVCLRLGLAEAVAAEADEPPVLLLDDPFSGLDPERRRRLTDGLGRRDQVLVS
                     MPDEAIVPPGATVWEVRDGAVVAR"
     gene            1786..2115
                     /locus_tag="VM638_00555"
     CDS             1786..2115
                     /locus_tag="VM638_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HVM10950.1"
                     /translation="MARSSRGEERGDREPRPIGDVLRGMRTAGPLGPGLALGHLVRRW
                     EELAGEELAGHTAPAALSGGTLIVRADSSGWAARTRFVAGELQRAAERVVGRGVVRSV
                     RVVVGDA"
BASE COUNT          298 a          828 c          912 g          278 t
ORIGIN      
        1 gcttcgctca ggccgtgggg caggtcgtga aggcggcctc ccgagacgag gctcgcccga
       61 tcctcaccgg cgtgctcctc gaggcgaacc gtgagggact gacgatggtc gcgaccgact
      121 cgtaccggct cgccgtgcgc gagctctcgg cgacgggaga gggcgaggcg aaggccctcg
      181 tccccgagcg cgcgatctcc gaggcgggac gggccgcggc gaccgaggag cgcgcggagg
      241 tccggatggt cctcggcgac tcccaggcct cattccgggt cggcggcgtc acgatgacct
      301 cgcggctgat cgacggggag ttcccgaact accgccagct cctccccgag ccgggcggga
      361 accgactgac ggccccgcgc caggagctgc tcgaggccgt ccgccgggtc gggctcctcg
      421 cccgcgagaa ctcaccggtg cggctggagc tcaacgcgct cggggtgcgc ctgacgtcgt
      481 cgtcccccga cctcggcggc gcggtcgagg tgctcgaggc gagctacgag ggcgacgagc
      541 tgacggcggc gttcaacccg acctacctcg gggacggcct cgccggcgtg cccgccgatc
      601 gggtctcgct cgagctgcgc gacggcctga agccggcgct cctgcggggg gagggcgacg
      661 atgccttcac ctacctcgtc atgccggtcc ggctgcccgc gccggtcgga tagcgcggac
      721 gagcagggcg ggtgcggctc gagcggctgg agctccgcga cttccggaac caccgcgaga
      781 cgcggctcga ggtgccgggc ggcctcgtgg cggccgtggg gccgaacgcc cagggcaaga
      841 cgaacctgct cgagggggcg tactacctga tgacgctggg ctcaccgcgc gtggcgaccg
      901 atcagccgct cgtgcgcgcc ggagcgacga gcgcctacct ccggggcgag atccggacgg
      961 cgtcgggccg cgcgctcgtg gaggtcgagg tgcgctccag cggcgcgaac cgcgtgcggc
     1021 tgaacggcag cggcatccgc cgcaagcgcg accttcggca acgggtgcgg gccgtgatgt
     1081 ccgtcccgga ggatctggcg gtggtccagg gcgaccccga ggagcggcgg cgcttcctcg
     1141 acgaggccgc cgagaccctg tggccggcga ccgaggccgc gcgccgtgcc ttcgaccgag
     1201 ccctgcgaca acgcaaccgg ctgctcaagg agcacgagcc cggcttccca cccgccgagc
     1261 tcggggtgtg ggatcgggag ctcgccacgc acggcgcggc ggtgacgagg aaccgagcgc
     1321 gcgccgtcga ccgactcgcg ccagccgctt ccgaggcgta ccgcgaggtc gccggtcacc
     1381 cgctgcgcgt cgcgtaccga ccctcggtcg agggcgagga cctcgaggcg tcgttcctcg
     1441 agcgtctcgc cgagcgacga ggcgacgagc tcatccggcg acgcaccctc gtcggaccgc
     1501 accgcgacga gctcgagctc ggggtcgggg agctcaccgc gcggaggttc gcctcgcacg
     1561 gggagtcgtc ggcggccgcc gtctgcctgc gcctcgggct cgccgaggcc gtcgccgccg
     1621 aggcggacga gccgccggtc ctgctcctgg acgacccgtt ctccgggctc gacccggaac
     1681 gtcgccggcg gctgaccgac gggctgggcc gccgcgatca ggtgctggtc tcgatgcccg
     1741 acgaagcgat cgttccgccg ggcgcgaccg tctgggaggt gcgcgatggc gcggtcgtcg
     1801 cgcggtgagg agcgcgggga ccgggagccg agaccgatcg gtgacgtcct ccgcgggatg
     1861 cgcaccgccg gcccgctggg ccccgggctc gcgctcgggc acctcgtccg gcggtgggag
     1921 gagctcgccg gggaggagct cgccgggcac acggcgccgg cggccctgag cggcgggacc
     1981 ctcatcgtcc gcgccgacag ctccgggtgg gcggcgcgga cccgcttcgt cgccggcgag
     2041 ctccagcgcg cggcagagcg ggtcgtgggg cggggggtcg tgcggtcggt ccgggtggtc
     2101 gtcggggacg cctgaggccg ggatcggcgc cggggagggg cccccctgat ggtagaatcg
     2161 aggtgctcct cgagcccccg cgtcgcgccc ctcgccgcat cgtgggctcg tcacgtccga
     2221 cggagggtgt gtgccgaagg caacagggaa gaacgcaaag acgacgaggt ccccccggcc
     2281 cgcgaagtcc acgaagaagg gcaagagcgg ctacac
//
LOCUS       DATLGG010000023         4133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_222632, whole genome shotgun sequence.
ACCESSION   DATLGG010000023 DATLGG010000000
VERSION     DATLGG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222632"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VM638_00560"
     CDS             complement(<1..579)
                     /locus_tag="VM638_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVM10951.1"
                     /translation="MRVMVWNVHGFRAGVRAVAAVVQEAQPDVLLLNEARFRWRLRRL
                     ARRLGMRAHHGMHGRRRVPNAILVRPPWRIVEGWSAPLSPTRRMKRRGLVAANVRHGG
                     ERLTVACVHLGVSDPERVRHAREVTDMLAGAPPPLVLGGDLNEGPDRPAARWIAERYF
                     DTFAGNGGDTFPATEPRARIDYVFVTEDLEVTA"
     gene            complement(599..1531)
                     /gene="arcC"
                     /locus_tag="VM638_00565"
     CDS             complement(599..1531)
                     /gene="arcC"
                     /locus_tag="VM638_00565"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HVM10952.1"
                     /translation="MTSRGRAVLALGGNALLRRGEDDTPDVMRSNARLAAERVADIAG
                     AGWEVVLTHGNGPQVGRILLQNEAARDQVHPLPLDVLGAESQGQIGYLLQVSIGDVFY
                     ERGMERPVATVLTLTRVPEDDPAFQQPTKPIGPYYDHVEAAGLAAERGWEMAEVTGGW
                     RRVVPSPRPYSIVEAPVIRQLAESGAIVIAAGGGGVPVVERGPRLVGVEGVIDKDRAA
                     GILARDVEAEVLAILTDVDGVYERYGQPDERVIDELTVDEAARLLEDLPEGSMRPKLE
                     AALGFVRDGGKLAVIASLDAAPEAIEGKAGTRIV"
     gene            complement(1528..2835)
                     /locus_tag="VM638_00570"
     CDS             complement(1528..2835)
                     /locus_tag="VM638_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HVM10953.1"
                     /translation="MTRVLIMGAAGRDFHNFNVVYRDDPDSQVVAFTATQIPFIDQRR
                     YPPELAGPRYPDGIPIHPESEVTRLIGELGVDEVVFAYSDVSHEHVMHAASIALAAGA
                     DFRLLGPDSTMLESEKPVVAICAVRTGSGKSQTTRAVVRALTAMGRRPAVVRHPMPYG
                     NLVAQRVQRFASFEDLDEANVTIEEREEYEPHLASGTVVYAGVDYEEILKKAQAEADV
                     VVWDGGNNHLPFFRPTVHFVVADPHRPGHETTYHPGEANLRRGDVIVINKVDTADPAA
                     VDAVEATARELNPSAVVIRAESPVTVDDPAAIAGKRVLVIEDGPTLTHGEMTYGAGVV
                     AARAGGAEIVDPREWAAGSLAAIYEHYPVGPVLPAMGYSPEQIAEMERTIEAVPCDVV
                     VVATPVDLTKIARFSKPSVRVGYELAEAEGGPTVASILAERLA"
     gene            complement(2886..3818)
                     /locus_tag="VM638_00575"
     CDS             complement(2886..3818)
                     /locus_tag="VM638_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM10954.1"
                     /translation="MKVGLVLPLFSGNPARVLEVAREAERLGFDGAFVFDHLFPPGAP
                     RDRPSLEAFTTLAAVGAATERLEIGTLVTRASLRPTGMLAKLGAWLDAATGGRVILGI
                     GTGDPLDFPEHEAYGIPILDRAGRRAQLDETVRALKALFAGETFSGGEHVPPLTGPLV
                     PAPVRPGGPPIWLGAQAERVIRMAARIADGWNGWGLEPKAFRAKVEILEEEAAAADRS
                     AEATWAGIALVGADEAETKELLERRRRRGLEDEAWVGTADELVAHLGGLADAGATWAV
                     LVPAGPKDRPALIAERVLPALRAPAVPGSAPSVG"
     gene            complement(3815..>4133)
                     /locus_tag="VM638_00580"
     CDS             complement(3815..>4133)
                     /locus_tag="VM638_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVM10955.1"
                     /translation="AGPTGEDVGRAAADGDPEAAAIVREVGRRLGEGLAGLVNILDPE
                     AVVVGGGVAELGEALLGPAREAFALAVEAPEHRPEVPILPAALGSDAGAIGAALLALE
                     TAS"
BASE COUNT          539 a         1558 c         1481 g          555 t
ORIGIN      
        1 ggccgtcacc tcgaggtctt ccgtcacgaa cacgtagtcg atcctggcgc gaggctcggt
       61 cgccgggaag gtgtcgccgc cgttgccggc gaaggtgtcg aagtagcgct cggcgatcca
      121 gcgggcggcg ggtcgatcgg ggccctcgtt gaggtcgccg ccgaggacga gcggcggcgg
      181 cgcgccggcc agcatgtcgg tgacctcccg ggcgtggcgg acccgttcgg gatccgagac
      241 gccgaggtgg acgcaggcga cggtcagccg ctccccgccg tgccgcacgt tcgccgcgac
      301 caggccgcgc cgcttcatcc gtcgcgtcgg cgacagcggc gccgaccacc cctcgacgat
      361 ccgccacggc ggccggacga ggatcgcgtt cggtacccgt cggcgtccgt gcatcccgtg
      421 gtgcgcccgc atccccaggc gccgcgcgag ccgcctcagc cgccaccgga accgcgcctc
      481 gttcagcagc aggacgtccg gctgcgcctc ctgcaccaca gccgccacgg cacgcacccc
      541 cgcccggaac ccatgcacgt tccagaccat cacccgcatg cggatgagcg tatcgacgct
      601 agacgatgcg tgtgccggcc ttgccttcga tggcttcggg agcggcgtcg agggaagcga
      661 tgaccgcgag cttgccgccg tcacggacga agccgagggc ggcttcgagc ttcgggcgca
      721 tggagccttc ggggaggtcc tcgaggagcc gcgcggcctc gtcgaccgtg agctcgtcga
      781 tcacccgctc atccggctga ccgtaccgct cgtagacccc gtcgacgtcc gtgaggatcg
      841 ccagcacctc ggcctcgacg tcgcgggcca ggatcccggc cgcgcggtcc ttgtcgatga
      901 caccctcgac gccgacgagg cgtgggcctc gttccaccac cggaacccct cccccgccgg
      961 ccgcgatcac gatcgcgccg gactcggcga gctggcggat cacgggcgcc tcgacgatcg
     1021 agtacgggcg cggcgacggg acgacccgcc gccagccgcc ggtgacctcg gccatctccc
     1081 agccgcgctc cgctgcgagc ccggccgcct cgacgtggtc gtagtacggc ccgatcggct
     1141 tcgtgggctg ctggaaggcc gggtcgtcct cgggcacgcg cgtcagcgtc agcaccgtcg
     1201 cgacgggacg ttccatcccg cgctcgtaga agacgtcccc gatcgagacc tggaggaggt
     1261 agccgatctg gccctggctc tcggcgccga gcacgtcgag cggcagcggg tggacctggt
     1321 cgcgcgcggc ctcgttctgc aggaggatcc gcccgacctg ggggccgttc ccgtgcgtca
     1381 acacgacctc ccagcccgcg ccggcgatgt ccgcgacgcg ctcggccgcg agccgcgcgt
     1441 tcgaccgcat cacgtcgggc gtgtcgtcct cgccgcgacg cagcagcgca ttgccgccga
     1501 gcgcgaggac ggcgcgtccg cgagaggtca cgccagccgc tcggcgagga tcgaggcgac
     1561 ggtcgggccg ccctcggcct cggcgagctc gtagccgacg cgcaccgacg gcttcgagaa
     1621 gcgcgcgatc ttcgtgagat ccaccggggt cgcgaccacc acgacgtcgc aggggaccgc
     1681 ctcgatcgtc cgctccatct ccgcgatctg ctccggcgag tagcccatcg cggggagcac
     1741 cggcccgacc gggtagtgct cgtagatcgc ggcgaggctc cccgccgccc actcgcgagg
     1801 gtccacgatc tcggcaccgc cggcgcgcgc cgcgacgacc ccggcgccgt acgtcatctc
     1861 cccatgcgtg agcgtcggcc catcctcgat cacgagcacg cgcttgcccg cgatcgccgc
     1921 cgggtcgtcc acggtgacgg gactctccgc tcggatcacc acggcgctcg ggttcagctc
     1981 gcgggccgtg gcctcgacgg cgtcgaccgc ggccgggtcg gcggtgtcga ccttgttgat
     2041 cacgatcacg tcaccccggc ggaggttcgc ctcccccggg tggtacgtcg tctcgtgccc
     2101 cgggcggtgc gggtcggcga cgacgaagtg gacggtcggg cggaagaacg gaaggtggtt
     2161 gttgccgccg tcccagacga cgacgtccgc ctcggcctgc gccttcttca ggatctcctc
     2221 gtagtccacg cccgcgtaga cgaccgtgcc gctcgcgagg tgcggctcgt actcctcacg
     2281 ctcctcgatc gtcacgttcg cctcgtcgag gtcctcgaac gacgcgaacc gctggacgcg
     2341 ctgcgccacg aggttcccgt acggcatcgg gtggcggacg acggccggcc gccggcccat
     2401 cgcggtcagc gcgcgcacca cggcgcgggt cgtctggctc ttgcccgaac cggtccggac
     2461 cgcacagatc gcgacgacgg gcttctcgct ctcgagcatc gtcgagtcag ggccgagcag
     2521 gcggaagtcc gcgccggcgg ccagggcgat gctcgccgcg tgcatcacgt gctcgtgcga
     2581 gacgtcggag taggcgaaga cgacctcgtc gacgccgagt tcgccgatca gccgcgtcac
     2641 ctcggactcg gggtggatcg ggatgccgtc ggggtagcgc ggccccgcga gctccggcgg
     2701 gtaccgacgc tggtcgatga aggggatctg cgtcgcggtg aacgcgacga cctggctgtc
     2761 gggatcgtcc cggtagacga cgttgaagtt gtggaagtcg cggccggccg cgcccatgat
     2821 caagaccctg gtcatcgatg ttcctccgag ttcgtgtggg agatgggcgc tcggcccggt
     2881 cgattctagc cgaccgaggg cgcggagccg ggcaccgcag gggcgcggag cgcgggcaac
     2941 acgcgctcgg cgatcaaggc cgggcggtcc ttcggtccgg ccgggacgag gacggcccat
     3001 gtcgcgccgg cgtcggccag gccgccgagg tgcgccacca gctcgtcggc ggtcccgacc
     3061 cacgcttcgt cctccaggcc ccggcgccgg cggcgttcga ggagctcctt cgtctccgcc
     3121 tcgtctgcgc cgacgagcgc gatcccggcc cacgtcgcct ccgccgatcg atcggcggcc
     3181 gccgcctcct cctcgaggat ctcgaccttg gcccggaacg ccttcggctc cagtccccag
     3241 ccgttccacc cgtcggcgat ccgcgcggcc atccggatca cgcgctcggc ctgcgcgccg
     3301 agccagatcg gcggaccgcc gggtcgcacc ggcgcgggca cgagggggcc ggtgagcggc
     3361 gggacgtgct cgccgccgga gaacgtctcg ccggcgaaga gagccttcag cgcgcggacg
     3421 gtctcgtcga gctgtgcgcg gcggccggcg cggtcgagga tcgggatccc gtaggcctcg
     3481 tgctcgggga agtccagcgg gtcgccggtc ccgatcccca ggatgacccg gcccccggtc
     3541 gccgcgtcga gccaggctcc gagcttcgcc agcatccccg tcggacgcag tgacgcgcgg
     3601 gtgacgagcg tgccgatctc gaggcgctcg gtcgcggcgc ccaccgccgc gagcgtcgtg
     3661 aacgcctcca gggaggggcg gtcccggggc gctcccggcg ggaagaggtg gtcgaagacg
     3721 aaggcgccgt cgaagccgag gcgctccgcc tcacgcgcga cctccaggac ccgcgcaggg
     3781 ttgcccgaga agaggggcag gacgaggccg accttcacga ggcggtctcc agcgcgagga
     3841 gcgccgcgcc gatcgccccg gcgtcgctgc cgagcgccgc gggaaggatc ggaacctccg
     3901 gccggtgctc cggagcctcg acggcgagcg cgaacgcctc ccgggcgggc cccagcagcg
     3961 cctcccccag ctcagcgacg ccgccaccga cgacgaccgc ctccgggtcg aggatgttca
     4021 cgagccccgc gagaccctcg ccgagacggc gaccgacctc tcgcacgatg gccgccgctt
     4081 cggggtctcc gtcggccgcc gcccgtccca cgtcctcgcc ggttggcccg gcg
//
LOCUS       DATLGG010000024         5574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_64229, whole genome shotgun sequence.
ACCESSION   DATLGG010000024 DATLGG010000000
VERSION     DATLGG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64229"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VM638_00585"
     CDS             <1..227
                     /locus_tag="VM638_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10956.1"
                     /translation="PLPRRPSGMPPPDTQAPGYTVRQVTRAEKAYVCPGCNQPIPVGV
                     VHLVVYRDDEPDLRRHWHTPCWRRELKLLR"
     gene            complement(231..1544)
                     /locus_tag="VM638_00590"
     CDS             complement(231..1544)
                     /locus_tag="VM638_00590"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVM10957.1"
                     /translation="MRRSLILCLTVAAGSTLLTGPTGAGADEPDAPACDQRAPTFRPP
                     LRLGNGYGYEPGIEMDSQGTIFVTAHKLSLVAEGDRLASWLWRSTDDGRSFQDMSGVD
                     GFPKLLWALEGDFAVDEKDRLYWVDTWAADNHFIRWSNRGTTVDFLRPFVASGQPVDD
                     RPWLAAHGDGYVYYMGNMAVGTPAHARLFVNRSTDGGQTFDPVGFSFPNSNWGFLDAD
                     PTSPYVYAVMDETNTSNMVAWVSPDRGSTWTRRVIAPVSPARAGSDLEFPSMAVSPVD
                     GTVYALWDNNSGIFLAESRDRGTTWTVHNVTPFAGGYSFPWVTVGPTGDVGIIFSADP
                     QSVAGNGYFVYGMVWRPGSDCLLDPENPGSMCTGPSRIYGRLQPGSTGDRASQADFFQ
                     VEMSADNALNVPWTDRNQGSIIEYTRQSAGPNMDGTPTCGLVGTP"
     gene            complement(1631..2659)
                     /locus_tag="VM638_00595"
     CDS             complement(1631..2659)
                     /locus_tag="VM638_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HVM10958.1"
                     /translation="MRWDNLRLDVTQADDRLTPALFEQGAVARTFDTPEFRGMTFYEV
                     QARSIINRVPDASHVPFRWTINPYRGCQHACGYCFARNTHTYLDMDYGEDFDRKVVVK
                     VNAPDRLRKELARASWKGEHIAMGTNVDPYQRAEGRYRLMRGILEALRDFANPFSILT
                     KGTLILRDLDLLREAAQVTDVGTNVSVGSVDREAWKLIEPGTPSPLKRLDACRALNEA
                     GIPCGVLMAPILPFLTDDDEHLEETIRAIAAAGATHVSPIALHLRPGAREWFLGWIGD
                     HRPDLLPRYEELYRGRTYAPKAYQHDLAERVAKLARRHGIGRTSARNRRRIADPPRPD
                     GPEQLSMA"
     gene            2715..3689
                     /locus_tag="VM638_00600"
     CDS             2715..3689
                     /locus_tag="VM638_00600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM10959.1"
                     /translation="MGHVEKNRRWWEAAAGSYQRQHGERLRRRAAAWGVWRIPESQVG
                     ILGEVEGLDVLELGCGAAQWSVALDRLGARPVGLDVAAAQLDAARRVLDREGRSFPLV
                     QASAEVLPFADGSFDLVLSDHGAMSFADPRRTVPEVARVLRPGGALVFCTDTPWHFVC
                     WDRSTQRVGTELREGYFGMRTGEDEETVQFQLPYGAWIELFREHGLEVERLAELRPPA
                     GARTTYEDWVPLEWARRWPAEQVWRAVRRSEEKVPDLVGVAEAAALLGWDKRRVATYV
                     ARGSFPAPVAELAGGRVWRRSDVRAFGERFRARRESRRRPANPRGILP"
     gene            3717..4100
                     /locus_tag="VM638_00605"
     CDS             3717..4100
                     /locus_tag="VM638_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HVM10960.1"
                     /translation="MAVKFLSEEWAAAVKEALNSSDAFKSAAGGQTARIQNVVTTSEG
                     EAKNYLTVEGGAADLGVGEVDSPDVTISQDYETAVKLWRNEITGTAAYMSGKIRVSGD
                     LMKLMQLQGVIGALPAAVSGIDVEY"
     gene            4148..4645
                     /locus_tag="VM638_00610"
     CDS             4148..4645
                     /locus_tag="VM638_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVM10961.1"
                     /translation="MSVEVVRSPRRRKTVQAERRGRTVVVTLPAGMTRAEERHWVDTM
                     VERLLRKERLQRLNGSQDLERRAQELNRRYFGGRLRWASIRYVRATRSRFASCTPEDR
                     TIRVSERVADLPSWVRDYVLVHELAHLRIAGHTPAFWRLVERYPLTERARGFLLALGP
                     DGPLG"
     gene            complement(4642..5256)
                     /gene="upp"
                     /locus_tag="VM638_00615"
     CDS             complement(4642..5256)
                     /gene="upp"
                     /locus_tag="VM638_00615"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HVM10962.1"
                     /translation="MRTVVDHPLAAHLLTRLRDRTTPPPVFRTLTRRLTAILALEATR
                     DLPAETRTVETPLEPAEGRELAGIVAIPILRAGLGMLEAVSELFPEVAVGYIGLERDE
                     ATLRPASYYEKLPTVEGRHVLLLDPMLATGGSAVRACEGVKAGRPASIRMVCVVAAPE
                     GIAALERAHPDVRIVAAAIDRGLSDRGFILPGLGDFGDRLYGTD"
     gene            5352..>5574
                     /locus_tag="VM638_00620"
     CDS             5352..>5574
                     /locus_tag="VM638_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HVM10963.1"
                     /translation="MRSIRVMRFGVVVLVLALATAACGGGGQRRGGGGGTAQGPCDPA
                     GNAAEDGVKVGIAFDVGGLGDQSFNDAAKR"
BASE COUNT          769 a         1927 c         2069 g          809 t
ORIGIN      
        1 cacccctccc gcgccgcccg agcgggatgc ccccgcccga tacgcaggcg ccgggctaca
       61 ccgtccgtca ggtcacgcgg gccgagaagg cgtacgtctg ccccggctgc aaccagccga
      121 tcccggtcgg ggtcgttcac ctcgtcgtct accgcgacga cgagcccgac ctccggcgcc
      181 actggcacac cccgtgctgg cggcgcgagc tcaagctgct gcgctgagcg ctacggcgtt
      241 ccgacgaggc cgcaggtcgg cgtcccgtcc atgttcgggc cggcggattg ccgcgtgtac
      301 tcgatgatcg acccctggtt ccggtccgtc cacggcacgt tcagcgcgtt gtccgcggac
      361 atctcgacct ggaagaagtc cgcctgcgac gcgcggtccc ccgtcgatcc cggctggagt
      421 cgcccgtaga tccggctcgg tccggtgcac atggatcccg ggttctccgg gtcgagcagg
      481 cagtcggagc ccggccgcca gaccatcccg tagacgaagt agccgttgcc ggcgacagac
      541 tgcggatcgg ccgagaagat gatgccgacg tcgccggtcg gtccgaccgt cacccagggg
      601 aacgagtagc caccggcgaa cggggtcacg ttgtgcaccg tccaggtcgt gccgcggtcg
      661 cgcgactcgg cgaggaagat gccgctgttg ttgtcccaca gcgcgtacac ggtgccgtcc
      721 accggggaga ccgccatgct cgggaactcg aggtcgctgc ccgcccgtgc ggggctgacg
      781 ggcgcgatca cccggcgcgt ccacgtgctg ccgcggtcgg gcgagaccca cgcgaccatg
      841 ttgctggtgt tcgtctcgtc catgaccgcg tagacgtaag gggaggtggg gtcggcgtcg
      901 aggaagcccc agttcgagtt cgggaaggag aaaccgaccg ggtcgaacgt ctgcccgccg
      961 tcggtcgagc ggttcacgaa gagccgcgcg tgcgctgggg tccccaccgc catgttgccc
     1021 atgtagtaga cgtagccgtc tccatgcgcc gcgagccacg ggcggtcgtc gaccggctgg
     1081 ccgctcgcga cgaagggccg gaggaagtcg acggtcgtcc cgcggttcga ccaccggatg
     1141 aagtggttgt ccgcggccca cgtgtcgacc cagtagagcc gatccttctc atccacggcg
     1201 aagtcgccct ccagcgccca caggagcttc gggaaaccat cgaccccgct catgtcctgg
     1261 aacgaccggc cgtcatcggt ggagcgccag agccaagagg cgagacggtc cccctcggcc
     1321 accagcgaca gcttgtgggc ggtcacgaag atggttccct gggagtccat ctcgatcccg
     1381 ggctcgtatc cgtacccgtt ccccaggcgg agcggggggc ggaaggtggg agcgcgctgg
     1441 tcgcaggcgg gagcatcggg ctcatcggct cccgcgcccg tgggcccggt gagcagcgtg
     1501 gagcccgccg cgacggtcag gcacaggatc agggaacgac gcatggcgag ctcctatcgg
     1561 acggtctctc taggtcttcg ccgccgaagg gccggttccc tgctccgtcg cccgatgtgg
     1621 agcgagcgcc tcaggccatc gagagctgct cgggcccgtc cgggcggggc ggatcggcga
     1681 tccgtctcct gttgcgggcc gacgttcggc cgatcccgtg gcggcgtgcg agcttcgcca
     1741 cccgctcggc gaggtcgtgc tggtacgcct tcggcgcgta cgtcctcccc cggtagagct
     1801 cctcgtacct cgggagcagg tcgggccggt ggtcgccgat ccagccgagg aaccactcgc
     1861 gcgcgccggg tcgcaggtgg agcgcgatcg gcgagacgtg cgtcgctccc gcagccgcga
     1921 tcgcgcggat cgtctcctcg aggtgctcgt cgtcgtcggt gaggaacggc aggatcggcg
     1981 ccatcaggac cccgcagggg atcccggcct cgttcagcgc ccggcaggca tccagccgct
     2041 tcagcgggct cggcgtcccg ggttcgatca gcttccacgc ttcgcggtcg acggacccga
     2101 ccgagacgtt cgtgccgacg tcggtgacct gcgcggcctc gcgcaggagg tcgaggtcgc
     2161 ggaggatcag cgtccccttc gtgaggatcg agaacggatt ggcgaagtcc cggagggcct
     2221 ccaggatccc gcgcatcagc ctgtatcgac cctccgcccg ctggtaggga tcgacgttcg
     2281 tccccatcgc gatgtgctcg cccttccagc tcgcccgcgc cagctctttc cgcagccggt
     2341 cgggcgcgtt caccttcacg accaccttcc ggtcgaagtc ctcgccgtag tccatgtcga
     2401 ggtacgtgtg cgtgttccgc gcgaagcagt aaccgcacgc gtgctggcag ccgcggtacg
     2461 ggttgatcgt ccagcggaag gggacgtgag aagcgtcggg gacccggttg atgatcgagc
     2521 gtgcttggac ctcgtagaag gtcatccccc ggaactccgg cgtgtcgaag gtgcgtgcga
     2581 cggcaccctg ctcgaagagg gccggcgtca atcggtcgtc ggcctgcgtg acgtcgagtc
     2641 ggaggttgtc ccagcgcacc ctggaacgat agaacgtgtg ttcgattgcc gcaaggggct
     2701 acgatgccgc tcggatgggc cacgtcgaga agaaccggcg ctggtgggag gcggccgccg
     2761 ggtcctacca gcggcagcac ggtgagcggc tgcggcggcg agcggcggcc tggggcgtgt
     2821 ggcggatccc ggaatcgcag gtcggcatcc tgggcgaggt cgaggggctc gacgtcctcg
     2881 agctcgggtg cggcgccgcg cagtggtcgg tcgcgctcga ccgcctcggc gcgcgacccg
     2941 tcggcctgga cgtcgcggcc gcgcagctgg acgccgcgcg ccgggtcctc gaccgcgaag
     3001 gccgctcttt cccgctcgtg caggcgagcg ccgaggtcct cccgttcgcc gacggctcgt
     3061 tcgacctcgt gctctccgac cacggcgcga tgtccttcgc cgacccgcgc cgaacggtgc
     3121 ccgaggtggc gcgggtcctg cggcccggcg gcgccctcgt cttctgcacc gacacgccgt
     3181 ggcacttcgt ctgctgggac cgctccacgc agcgcgtcgg gaccgagctc cgcgagggct
     3241 acttcgggat gcgcaccggc gaggacgagg agaccgtgca gttccagctc ccgtacgggg
     3301 cgtggatcga gctcttccgc gagcacgggt tggaggtcga gcggctcgcc gagcttcgcc
     3361 cgcccgcggg cgcgaggacg acgtacgagg actgggtccc gctcgagtgg gcgcgccggt
     3421 ggccggcgga gcaggtctgg cgcgccgtgc gtcgctcgga ggagaaggtg cccgacctcg
     3481 tgggggtggc ggaggcggcg gccctgctcg gctgggacaa gcgccgggtc gcgacctacg
     3541 tggcccgcgg ctccttcccg gcgcccgtcg ccgagctggc cggtggccgc gtctggcgtc
     3601 ggagcgacgt ccgggcgttc ggcgagcgct tccgggcgcg gcgggagtcg cggcgacggc
     3661 cggcgaaccc tcgcggtatc ctcccgtgac cgaacgccca gacgaggagg agatccatgg
     3721 ccgtgaagtt cctgtccgag gagtgggccg cggcggtcaa ggaggccctg aactcgagcg
     3781 acgccttcaa gagcgccgcc ggcgggcaga ccgcgcggat ccagaacgtc gtcaccacct
     3841 ccgaggggga ggcgaagaac tacctgacgg tggagggcgg cgcggctgac ctcggggtcg
     3901 gcgaggtcga cagccccgac gtcacgatca gccaggacta cgagacggcg gtcaagctct
     3961 ggaggaacga gatcaccggc accgccgcct acatgagcgg caagatccgg gtcagcggcg
     4021 acctcatgaa gctgatgcag ctccaaggcg tgatcggcgc cctcccggcg gcggtctccg
     4081 gcatcgacgt cgagtactga gcgttcccgg accgacggtg tcggggcccg cgcctacgat
     4141 gggggccatg tcggtggagg tcgtgcgcag cccccggagg cggaagaccg tccaggccga
     4201 gcggcgcggc cggacggtcg tcgtgaccct cccggccggc atgacccgcg ccgaggagcg
     4261 tcactgggtg gacacgatgg tcgagcggct cctgcgcaag gagcgcctcc agcggctgaa
     4321 cggctcgcag gatctcgagc gccgggcgca ggagctcaac cgccgctact tcggtggccg
     4381 cctccgctgg gcctcgatcc gctacgtgcg agccacccgg tcgcggttcg cctcgtgcac
     4441 gcccgaggac cggacgatcc gcgtgtccga gcgcgtcgcc gacctgccgt catgggtccg
     4501 cgactacgtg ctcgtccacg agctcgcgca cctgcgcatc gcgggccaca cgccggcctt
     4561 ctggcgtctc gtcgagcggt acccgctcac cgagcgcgcc cgcggcttcc tcctcgcgct
     4621 ggggccggac gggccgctcg gctagtcggt cccgtagaga cggtcgccga agtccccgag
     4681 gcccggcagg atgaagccgc gatcgctcag gccgcggtcg atcgcggctg cgacgatgcg
     4741 gacgtccgga tgcgctcgct ccagcgcggc gatcccctcg ggtgccgcca cgacgcagac
     4801 catccggatc gacgctgggc gcccggcctt cacgccttcg cacgcgcgga cggcggatcc
     4861 cccggtggcg agcatcgggt cgaggaggag cacgtggcgt ccctcgaccg tcgggagctt
     4921 ctcgtagtag gaggcgggtc ggagcgtcgc ctcatcgcgc tcgaggccga tgtagccgac
     4981 cgcgacctcc gggaagagct cggacacggc ctcgagcatc ccgaggcccg cccgcaggat
     5041 cgggatcgcg acgatgccgg cgagctcgcg cccctcggcg ggctccagcg gcgtctcgac
     5101 cgtgcgcgtc tccgccggga ggtcgcgtgt cgcctccagc gcgaggatcg ccgtgagccg
     5161 ccgcgtcagc gtgcggaaca cgggcggcgg ggtcgtgcga tcccgcaagc gggtgagcag
     5221 gtgcgcggcc agcgggtggt cgacgacggt gcgcatcgcc gggatggtac cggttcgtcc
     5281 ccccgccgtt tgacgccgtg gaaccagcgg accagaatcg tcgcgctgac ggtgacggag
     5341 aagggggcgg gatgcggtcg atccgtgtga tgaggttcgg tgtggtggtc ctggtgctcg
     5401 cgctggcgac ggcagcgtgc gggggcgggg gacagcggcg cggcggcggc ggcggaacgg
     5461 cacagggacc gtgcgacccc gccggcaacg cggcggagga cggggtgaag gtcggcatcg
     5521 ccttcgacgt gggcggtctc ggcgaccagt cgttcaacga cgcggcaaag cgcg
//
LOCUS       DATLGG010000025         3979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_175491, whole genome shotgun sequence.
ACCESSION   DATLGG010000025 DATLGG010000000
VERSION     DATLGG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175491"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VM638_00625"
     CDS             complement(<1..788)
                     /locus_tag="VM638_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10964.1"
                     /translation="MSERHEKKRRFAPLVAMLSIAMVGWMAPAGANHSIMKPGELPKW
                     MQPDAGDGSVQEAQPRSGLREVGFHGLENKGFNTDIAPWASMNGGVYAATGTWGSFPV
                     LGAEADGDACPSERDPSGQSRSGVWLIEATDPANPEIVAKLPTIPGAQNNDPKVQHLP
                     TVDGGRDLLIHGLEPCGVEGVLHQIPGSPFVDVATTLQREAKLDQTGFQVYDVSDPSN
                     PVRLGTWNNGGIGTHNLLPFTHGDRSYVAAVFNKVDYIGAEESEL"
     gene            complement(922..1548)
                     /locus_tag="VM638_00630"
     CDS             complement(922..1548)
                     /locus_tag="VM638_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HVM10965.1"
                     /translation="MDDHQLIWQPTLLSAAEPLDFDRRFTRLTRVQLDDRSWVDHAPG
                     WVSGSDELFAQVLEGRGWEQRARTMYDQKVREPRLTAPWNARSGERLEPPILEEIRQA
                     LSARYDRDFDSVGFNLYRDGRDSVAWHGDRIPKEIEDPIVALVSLGEPRRFLLRPKGG
                     GISHTFLLGRGDLLVTGGRTQRDWDHAVPKVAKAGPRISLAYRHGFRS"
     gene            complement(1615..2514)
                     /locus_tag="VM638_00635"
     CDS             complement(1615..2514)
                     /locus_tag="VM638_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM10966.1"
                     /translation="MLRPLRVGIQLPEVERVVRWPEVVAMARTAEEVGFDSIWVGDHL
                     LYRGHGRPERGPWEAWTTLAALAAVTSRVRVGPLVACAAFHPPGIVAKMAATLAEVSV
                     GRFVLGIGAGWNREEFEAFGIPYDRRVDRFEEAFGIVRRLLAGERVTVQGRFHRTCDA
                     VILPSPRHRPTLMIGSNGPRVLGIALPHVDAWNTWFSWTGNTPEGFEARSHAVTTACR
                     QAGRDPSEVARSVCVLVALDREAAERPLTDEAPPVEGPPEAIAHVLRAYAEAGADEVI
                     VVPTPNDETSIRALAPALELLDR"
     gene            complement(2534..2791)
                     /locus_tag="VM638_00640"
     CDS             complement(2534..2791)
                     /locus_tag="VM638_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10967.1"
                     /translation="MQTRHGTTRGHRPLRHFLIRAAWVAVPLVVAGELGHSVLQALGR
                     EIAHHFFHIVFGVGAAVVFALYVAIDIRRHGWPSFTLRRGS"
     gene            complement(2792..3208)
                     /locus_tag="VM638_00645"
     CDS             complement(2792..3208)
                     /locus_tag="VM638_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HVM10968.1"
                     /translation="MGDGYGGRGVARAYGFIFGPAYLAVALVELLTMRGGLSIGGFEA
                     LSFGGLHNVIHWATGLAVLGSAIAGEGPARTVARVVGIVFLLVTIWNIVAPDAYASFV
                     GIEGDLPITYTIVHAATAAGALFAGFASYKGEAARA"
     gene            3472..>3979
                     /locus_tag="VM638_00650"
     CDS             3472..>3979
                     /locus_tag="VM638_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM10969.1"
                     /translation="MDDAREATDAGLVVALARSRQDALAEVYRRHGGALFGMARRVIG
                     HESLAEEVVQEVFLHLWRHPDRFDPGRGSLRSYLLAIGHARSVDAVRSEVARRRREEK
                     DAVQDVREDAGAEERVAARLDAARSVGDALDSLPDEERRPIELAYFGGRTYREVAAFL
                     GEPEGTIKS"
BASE COUNT          583 a         1402 c         1417 g          577 t
ORIGIN      
        1 agctccgact cctccgcgcc gatgtagtcg accttgttga acacggccgc gacgtaggag
       61 cggtcaccgt gcgtgaacgg gaggaggttg tgcgtcccga tcccgccgtt gttccaggtg
      121 ccgagccgga ccgggttcga tgggtccgag acgtcgtaga cctggaagcc cgtctggtcg
      181 agcttcgcct cgcgctggag ggtcgtggcg acgtcgacga acggcgaacc ggggatctgg
      241 tgcagcacgc cctcgacgcc gcacggctcg aggccgtgga tcaggaggtc cctgccgccg
      301 tcgacggtcg ggaggtgctg gaccttcggg tcgttgttct gggcaccggg gatcgtgggc
      361 agcttcgcga cgatctcggg gttggccggg tcggtggcct cgatcagcca gacaccggat
      421 cggctctggc cgctcgggtc acgctcggag gggcaggcgt ccccatccgc ctcggcgccg
      481 aggaccggga agctccccca ggtgcccgtc gcggcgtaga cgccaccgtt catcgacgcc
      541 catggcgcga tgtcggtgtt gaaccccttg ttctcgaggc cgtggaagcc gacctcgcgg
      601 agcccgctgc gaggctgagc ctcctgcacg gacccgtcac cggcgtcggg ctgcatccac
      661 ttcggcagct cgcccggctt catgatgctg tggttcgctc ccgcgggggc catccagccg
      721 accatcgcga tcgacagcat ggccaccagg ggagcgaacc gccgcttctt ctcgtggcgt
      781 tccgacatcg gaaaccacct cctcgtgcgg agcgagggtc cgggtcgggg gcccggtgcc
      841 gcgctcctcg gctacatcca gcggcacctg aatcctaccc agatggtcgg atttcatccc
      901 cccttggggc cttcgaggct tctagctgcg gaagccgtgg cggtaggcga ggctgatccg
      961 cgggcccgcc ttcgcgacct tggggacggc gtggtcccag tcgcgctgcg tgcggccccc
     1021 ggtgacgagg aggtccccgc gccccagcag gaaggtgtgg gagatgccgc cccccttcgg
     1081 gcggagcagg aagcgccggg gctccccgag ggagacgagg gcgacgatgg gatcctcgat
     1141 ctccttcggg atgcggtccc catgccaggc gacgctgtcg cggccgtcgc ggtagaggtt
     1201 gaaccccacg gagtcgaagt cgcggtcgta ccgcgccgag agcgcctgcc ggatctcctc
     1261 gaggatcggg ggctcgagcc gctccccgga cctcgcgttc cacggcgccg tgaggcgcgg
     1321 ctcccggacc ttctggtcgt acatcgtccg ggcccgctgc tcccagccgc ggccctcgag
     1381 gacctgggcg aagagctcgt cggacccgct gacccacccg ggagcgtggt cgacccagga
     1441 gcgatcgtcg agctggacgc gcgtcagccg cgtgaagcgg cggtcgaagt cgagcggctc
     1501 cgctgccgac agcagggtcg gttgccagat gagctggtgg tcatccacgc cttccatctt
     1561 tcccatcggc gcggtcagcg agcggccctg gcgagcgcgc cgctcggtgc ccgcctagcg
     1621 gtcgagcagc tcgagggcgg gcgcgagggc gcggatcgac gtctcgtcgt tcggcgtcgg
     1681 cacgacgatc acctcgtcgg cgccggcctc agcgtaggca cggagcacgt gggcgatcgc
     1741 ctcgggcggt ccctcgaccg gcggtgcctc atcggtcagc ggccgctcgg cagcctcgcg
     1801 gtccagcgcc acgaggacac agacgctgcg ggcgacctcc gacggatcgc ggccggcttg
     1861 ccggcatgcc gtcgtgacgg catggctccg ggcttcgaat ccctccggcg tgttcccggt
     1921 ccagctgaac cacgtgttcc acgcgtcgac gtgcggcaac gcgatgccga ggacgcgcgg
     1981 accgttcgac ccgatcatga gcgtcggacg gtgccgtggc gagggaagga tgaccgcgtc
     2041 gcacgtgcgg tggaaccggc cctgcaccgt cacgcgctcg ccggcgagga ggcgccggac
     2101 gatcccgaac gcctcctcga accggtcgac acggcgatcg tacgggatcc cgaacgcctc
     2161 gaactcctcc cggttccagc ccgcgccgat gccgaggacg aagcgaccga cgctcacctc
     2221 ggcgagcgtc gcggccatct tcgccacgat ccccggcggg tggaaggcgg cgcaggcgac
     2281 gagcggcccc acgcggacgc ggctcgtcac cgccgccagg gccgccagcg tcgtccacgc
     2341 ctcccatggt ccgcgctccg gacgtccgtg cccccggtag aggaggtgat cgccgaccca
     2401 gatcgagtcg aaccccacct cctcggcggt gcgggccatc gcgaccacct cgggccaccg
     2461 cacgacgcgc tccacctcgg gaagctggat cccaacccgg agggggcgaa gcatgcgcgc
     2521 gactctagcc cgctcagctc ccgcgccgaa gcgtgaagct cggccacccg tggcgacgga
     2581 tgtcgatcgc cacgtacagg gcgaagacga ccgccgcccc cacgccgaac acgatgtgga
     2641 agaagtggtg ggcgatctct cggcccaggg cctgcaacac cgaatgcccg agctccccgg
     2701 cgacgacgag gggcaccgcc acccacgccg cgcggatgag gaagtggcgc agcggccggt
     2761 ggcccctcgt cgttccgtgc cgggtctgca tctaggctcg cgccgcctcg cccttgtagc
     2821 tcgcgaaccc tgcgaacagg gcccctgcgg cggtggccgc gtgcacgatc gtgtacgtga
     2881 tcggaaggtc tccctcgatc cctacgaagc tcgcgtaagc atcaggcgcc acgatgttcc
     2941 agatcgtgac gagaaggaag acgatgccca ccacgcgagc gacggttcgc gccggcccct
     3001 cgcccgctat ggccgagccg aggacggcca aacctgtggc ccagtggatc acgttgtgca
     3061 gcccaccgaa ggacaatgcc tcgaacccgc cgatcgagag cccgcctcgc atcgtgagga
     3121 gctccacgag cgccacggcc aagtaggcgg gaccgaagat gaaaccgtag gcgcgggcta
     3181 cgccgcggcc cccatagccg tcccccatag ctatccctcc ttctgctccc ctgtcgcggc
     3241 catcggggag gagtcgtctt ggtcgccgcg ccggcggaga gcctgcccac tcggacatgc
     3301 ttcgaaaccg acgccgctgc tgggggtagc cacacggcat ccgagggggc cggtggcgcc
     3361 gaagacccag tgatgtggga tgcagagcgc gggttcgcgc cgaggccgag ggtccttcgg
     3421 gggcgtcacc tccccggtcg gcgaggcacg tttcgtgggt atcgtctggc cgtggacgat
     3481 gcccgggagg ccaccgacgc gggcctggtg gtagcgctcg cccggtcgag gcaggacgcc
     3541 ctcgccgagg tgtaccgtcg gcatggagga gcgctgttcg ggatggcacg tagggttatc
     3601 ggccatgagt ccctggcgga ggaagtcgtc caagaggtct tcctgcatct ctggcgacac
     3661 cccgatcggt tcgaccccgg tcgtgggagc ctgcggagct acctcctcgc gatcggtcac
     3721 gcgcgcagcg tcgatgcggt ccggtccgag gtggcccgtc gccggcgcga ggagaaggat
     3781 gccgtccagg acgtgcgcga ggacgcagga gcggaggaac gcgtcgccgc ccggctcgac
     3841 gcggctcggt ccgtcgggga cgcgctcgac tcccttccag acgaggaacg ccggccgatc
     3901 gagctggcct acttcggggg ccggacgtac cgggaggtcg cggccttcct cggcgagcca
     3961 gaagggacga tcaagagcc
//
LOCUS       DATLGG010000026         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_96886, whole genome shotgun sequence.
ACCESSION   DATLGG010000026 DATLGG010000000
VERSION     DATLGG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96886"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VM638_00655"
     CDS             complement(<1..540)
                     /locus_tag="VM638_00655"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HVM10970.1"
                     /translation="MRELPRRILRATGTTLLVVGFVLVAYIVWLLWGTGIYTGRAQQE
                     LRDGFNRRVAAAEQEAPVPVPGEAFAVLQIPAIDLDVIVLEGTAIEDLKRGPGRYRET
                     DYPWEDTGTVGIAGHRTTYGAPFWSLNEVRPGDEITLHTQRGRFRYEVTDTRDVAPSE
                     VSVLDPTEEPTLVLTTCSPR"
     gene            673..1830
                     /locus_tag="VM638_00660"
     CDS             673..1830
                     /locus_tag="VM638_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HVM10971.1"
                     /translation="MEIEIGKGKIGRRAYGFDEVAIVPSRRTRDPDDVDISWEIDAYR
                     FDLPLMASAMDAVVSPTSAAEIGRLGGLAVLNLEGLQTRYEDPDPIFEEIAELPDDKA
                     TRRMQELYREPIKEELIGQRIRELKEAGIVAAASLTPQRTERYAKLVLEAELDILVIQ
                     GTVVSAEHVSTRTEPLNLKRFIATFDLPVIVGGCASYSTALHLMRTGAIGVLVGVGPG
                     QACTSRGVLGIGVPQATAIADAAAARMEHLRETGRYAHVIADGGMRTGGDIAKAIACG
                     ADAVMIGSPLAKAHEAPGRGYHWGMATFHPELPRGARVKTRQIGSLQEILTGPAHEND
                     GTLNLFGALRMSMATTGYANVREFQKAEVLVAPSIRTEGKDLQRQQGVGMA"
     gene            1827..3365
                     /gene="guaA"
                     /locus_tag="VM638_00665"
     CDS             1827..3365
                     /gene="guaA"
                     /locus_tag="VM638_00665"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HVM10972.1"
                     /translation="MTADPRPVLVVDFGAQYAQLIARRVRECHVYSEIVPFDISPEEV
                     AARRPAGIILSGGPASVYEQGAPALDPRLLGLGAPVLGICYGQQAMAQVLGGEVAPTG
                     VREYGKTDLEATDPGLLLRELPAEQSVWMSHADAVVRPPEGFRVTARTGAIPIAAMED
                     PERGLYAVQFHPEVAHTPHGTEMLKRFLYDACGLLPDWTPVNVIDRAVERIRTQVGSG
                     RVLCALSGGVDSAVAALLVYRAVGDQLTCVFVDTGLLREGEPEQVEETFARHFQVPLV
                     HVKAEDRFLARLEGVEDPELKRKRIGEEFIRVFEEVAREHQDARFLVQGTLYPDVIES
                     GSRTAAKIKSHHNVGGLPEVMDLDLVEPLRDLFKDEVRRVGEELGLPEEIVWRQPFPG
                     PGLAVRIVGEVSRDRLAMVRGADRIVQEELRRGGLGREIWQAFAVLLTVRSVGVMGDG
                     RTYQHPVILRAITSEDAMTADWARLPHEVLEAVSSRIVAEVPGVNRVAYDISSKPPAT
                     IEWE"
BASE COUNT          484 a         1219 c         1220 g          476 t
ORIGIN      
        1 cctcggcgag caggtcgtca gcacgagcgt cggctcctcg gtcggatcga gcaccgacac
       61 ctccgacggc gcgacgtcgc gcgtgtccgt cacctcgtag cggaacctcc cgcgttgcgt
      121 gtgcagcgtg atctcgtcgc cggggcggac ctcgttcagc gaccagaacg gcgccccgta
      181 cgtggtgcgg tgcccggcga tcccgaccgt gccggtgtcc tcccagggat agtcggtctc
      241 gcggtaccgc ccgggcccgc gcttcaggtc ctcgatcgcc gtcccctcga gcacgatcac
      301 gtcgaggtcg atcgccggga tctggagcac ggcgaaggcc tcgccgggga cggggacggg
      361 ggcctcctgt tcggcggcgg cgacccggcg gttgaagccg tcgcggagct cctgctgggc
      421 ccggcccgtg tagatcccgg ttccccacag gagccagacg atgtaggcga cgaggacgaa
      481 cccgacgacc agcagggtcg tcccggtcgc gcgcaggatc cgccggggga gctccctcac
      541 cgctccatta tccgcgaggg tcgccccgct ccgtggagcg gctgacgcgg cgtggtaccg
      601 tgaggccgat cacgtcgaga gccggcgcca gccgcttccc cggtcgcgtc cggccgaacc
      661 gggggaggtc aggtggagat cgagatcggc aaggggaaga tcgggcgccg ggcgtatggg
      721 ttcgacgagg tggccatcgt gccgtcccgc cggacgcgcg accccgatga cgtcgacatc
      781 tcctgggaga tcgacgccta ccgcttcgac ctgccgctca tggcctccgc gatggacgcc
      841 gtcgtctcgc ccacgagcgc ggccgagatc ggacggctcg gcggcctcgc ggtcctgaac
      901 ctcgagggcc tccagacgcg ctacgaggac cccgacccga tcttcgagga gatcgcggag
      961 ctccccgacg acaaggcgac ccggcggatg caggagctgt accgcgagcc gatcaaggag
     1021 gagctgatcg ggcagcggat ccgcgagctg aaggaagccg ggatcgtcgc ggcggcctcc
     1081 ctcacccccc agcggaccga gcgatacgcg aagctcgtcc tcgaggccga gctcgacatc
     1141 ctcgtgatcc agggcacggt cgtctccgcg gagcacgtct ccacgcggac cgagcccctc
     1201 aacctcaagc gcttcatcgc gaccttcgac cttccggtga tcgtcggggg atgcgcgtcg
     1261 tactcgaccg cgctgcacct gatgcgcacc ggcgcgatcg gcgtcctcgt gggggtcggc
     1321 cccgggcagg cttgcacgag ccgcggggtc ctcgggatcg gggtgccgca ggcgaccgcg
     1381 atcgccgacg cggccgccgc gcggatggag catctgcgcg agaccggccg ctacgcgcac
     1441 gtgatcgccg acggcggcat gcgcaccggc ggcgacatcg cgaaggcgat cgcctgtggg
     1501 gccgacgcgg tcatgatcgg gtcgccgctc gcgaaggcgc acgaggcgcc gggacgcggc
     1561 taccactggg ggatggcgac gttccacccc gagctccccc gcggtgcgcg cgtgaagacg
     1621 cgccagatcg gctccctcca ggagatcctg accggcccgg cgcacgagaa cgacgggacc
     1681 ctcaacctgt tcggcgcgct gcggatgtcg atggcgacga ccggctacgc gaacgtccgc
     1741 gagttccaga aggcggaggt cctcgtcgcg ccgtcgatcc ggaccgaggg gaaggatctc
     1801 cagcgccagc agggcgtggg gatggcgtga ccgcggaccc gcgcccggtc ctcgtcgtcg
     1861 acttcggggc gcagtacgcg cagctgatcg cgcggcgcgt ccgcgagtgc cacgtgtact
     1921 ccgagatcgt cccgttcgac atctcgcccg aggaggtcgc ggcacgtcgc cccgccggca
     1981 tcatcctttc gggcgggccg gcctccgtct acgagcaggg cgcgcccgcg ctcgacccgc
     2041 ggctgctcgg actcggcgcc ccggtcctgg ggatctgcta cggccagcag gcgatggcgc
     2101 aggtcctcgg cggcgaggtc gcgccgaccg gcgtgcggga gtacggcaag accgacctcg
     2161 aagccaccga cccggggctg ttgctccggg agctgccggc cgagcagagc gtctggatga
     2221 gccacgccga cgcggtcgtc cggccgccgg aggggttccg tgtgaccgcc cggaccggcg
     2281 cgatcccgat cgcggcgatg gaggaccccg agcgaggcct ctacgccgtc cagttccatc
     2341 cggaggtcgc ccacaccccg cacggcaccg agatgctgaa gcgcttcctc tacgacgcct
     2401 gcggcctgct gcccgactgg accccggtca acgtgatcga ccgcgccgtg gagcgcatcc
     2461 gcactcaggt ggggtcgggg cgcgtgctgt gcgctctctc gggcggggtc gactcggcgg
     2521 tcgcggccct gctcgtctac cgcgccgtcg gggaccagct cacctgcgtc ttcgtcgaca
     2581 ccggcctcct gcgggagggg gaacccgagc aggtcgagga gacgttcgcc cgccacttcc
     2641 aggtgccgct cgttcacgtc aaggccgaag accgcttcct cgcgcgcctc gaaggggtcg
     2701 aggatccgga gctcaagcgc aagcgcatcg gcgaagagtt catccgcgtc ttcgaggagg
     2761 tcgcgcggga gcaccaggac gcgcggttcc tcgtccaggg cacgctctac ccggacgtga
     2821 tcgagtccgg cagccgcacc gcggcgaaga tcaagagcca ccacaacgtc ggcggcctgc
     2881 ccgaggtgat ggacctcgac ctcgtcgagc cgctccgcga cctcttcaag gatgaggtcc
     2941 gtcgcgtcgg cgaggagctc ggcctcccgg aagagatcgt ctggcgtcag cccttcccgg
     3001 gacccgggct cgccgttcgg atcgtggggg aggtctcgcg ggatcgcctc gcgatggtgc
     3061 gcggggccga tcggatcgtg caggaggagc tccgccgcgg cgggctcggt cgcgagatct
     3121 ggcaggcgtt cgccgtcctg ctgaccgtcc gcagcgtcgg ggtgatgggc gacgggcgca
     3181 cctaccagca cccggtcatc ctccgggcga tcaccagcga agacgcgatg accgccgact
     3241 gggcgcgtct cccgcacgag gtgctcgaag ccgtctcttc ccgcatcgtc gccgaggtcc
     3301 ccggggtcaa ccgggtcgcc tacgacatct cctcgaagcc gcccgcgacg atcgagtggg
     3361 agtgagcgcc gctagccctg cttcggcctg agcatcgtc
//
LOCUS       DATLGG010000027         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_222733, whole genome shotgun sequence.
ACCESSION   DATLGG010000027 DATLGG010000000
VERSION     DATLGG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222733"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VM638_00670"
     CDS             complement(<1..613)
                     /locus_tag="VM638_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVM10973.1"
                     /translation="MSRVLLIGWDGADWRILDPMLEAGLLPNLASLVRRGAKGVLRST
                     VPNHSWSAWPSFLTGLDPANHGVYDIFERDFSTTKSLPVTYRSIKEPTILPDLADAGA
                     TTVMTNVPLTFPPPRVEGKLVSGGVLPKWRAFTYPEGLAAELEVAGAPYPINGMSWTT
                     FRNRPEPFLAEAMDITAARIRANEHLLSTTEWRFACLVYVATDR"
     gene            complement(851..1483)
                     /locus_tag="VM638_00675"
     CDS             complement(851..1483)
                     /locus_tag="VM638_00675"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HVM10974.1"
                     /translation="MTAAAPRAARLAETLRTRPGPRRALNVLSILLLVGGIALFAQPF
                     ITNLYANWRQSSLQDQLGSAANRNAYAGGALREGDPVTRLRIPKLGVDTIVVEGTTLS
                     ALRAGSGHYVQTPLPCEPGNSAIAGHRTTYSKPFARTHELERGDRIVLDTPVGRCVYR
                     VHDTWVTLPTDATVLAPARGSWLTLTTCDPPGSARERLIVRAKLVRSTPA"
     gene            complement(1480..>2538)
                     /locus_tag="VM638_00680"
     CDS             complement(1480..>2538)
                     /locus_tag="VM638_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10975.1"
                     /translation="FRTISYTAVSDGSGIRITLGAEGQVDRGGAITGAKVTSKPRSEG
                     FAAGVLGSDEATVSTKAPPDGPAKTAAGTEGAQTVGPFEVSGHSYEARSKSEAEDGTP
                     SSTNTATFGGLSIGGGAEAPLGALVEISPTGTSAGANAKGKNVTSASSSTGFIVTLSL
                     SAVALKEAAEQIQEPLCGGLAERNAQLGEVCNAAFSSTEQRVEFLRLEIGEGNVACTW
                     DGKTPDAKGSGATVTPFLLGQALPAISPTSEPVTLGVGTPLETTFGAGSFSKETEDAG
                     SNGTDKVDAIAGGAFARLLGGQVDVRSSTSTCSIAAPMSTDEVLAATGSVVLWYLIGG
                     AALVAASLGLRRFLRPTP"
BASE COUNT          339 a          885 c          952 g          362 t
ORIGIN      
        1 cccggtcggt cgcgacgtac acgaggcacg cgaagcgcca ctcggtcgtc gagagcaggt
       61 gctcgttggc gcggatccgc gccgccgtga tgtccatcgc ctcggcgagg aacggctccg
      121 gccggttccg gaaggtcgtc cacgacatcc cgttgatcgg gtacggagcg cccgcaacct
      181 cgagctctgc ggcgagcccc tcggggtagg tgaacgcacg ccacttcggg aggacccccc
      241 cggagacgag cttgccctcg acgcgcggcg gcgggaacgt gagcgggacg ttcgtcatga
      301 cggtcgtcgc gcccgcgtcg gcgaggtccg gcaggatcgt cggctccttg atcgagcggt
      361 aggtgaccgg gaggctcttc gtcgtcgaga agtcccgctc gaagatgtcg tacacgccgt
      421 ggttcgcggg gtcgagcccg gtcaggaacg acggccacgc ggaccaggag tggttcggga
      481 ccgtcgagcg caggaccccc ttcgcgccgc ggcggacgag cgaggccagg ttcggcagca
      541 gcccggcctc gagcatcggg tcgaggatcc gccagtcggc cccgtcccac ccgatcaaca
      601 gcacgcgcga catccggcgt agtctagccg ccccctcgag tgccggcctt cccgaggcgg
      661 tcccaggtgg ggcggttggg cggcggcctc cggtgtcgtg cgggcgggcg gaggcgcgcc
      721 tctggccgcg cccgctggga gcaacggagc cgaggtgttc atgcaggcgt cgcggtcggg
      781 cgcggcctgc aagcgccgga gcccgccggc cggccgtccc gggcgtgccg gacacagcga
      841 gcggccgctg tcaggcgggg gtggagcgga cgagcttggc gcggacgatc aggcgctccc
      901 gggcgctgcc ggggggatcg caggtggtga gcgtcagcca ggagcccctg gccggggcga
      961 ggacggtcgc gtcggtcggc agcgtcaccc aggtgtcgtg gacgcgatac acgcagcgcc
     1021 cgaccggcgt gtcgaggacg atgcggtccc ctcgctccag ctcgtgcgtg cgcgcgaagg
     1081 gcttcgagta cgtcgtccgg tgcccggcga tcgccgagtt ccccggctcg cagggcagcg
     1141 gggtctggac gtagtgcccg gatcccgcgc gcagcgccga gagcgtggtt ccctcgacga
     1201 cgatcgtgtc gacaccgagc ttcgggatcc gaagccgcgt cacggggtca ccctcacgga
     1261 gcgcgccacc cgcgtaggcg ttgcggttgg cggccgagcc gagctggtcc tggaggctcg
     1321 actggcgcca gttcgcgtac aggttcgtga tgaagggctg ggcgaacagc gcgatgccac
     1381 cgacgaggag caagatcgag agaacgttga gggcgcgtcg cgggccgggc cgggtccgaa
     1441 gggtctcggc gaggcgggcg gcgcgcgggg ccgccgccgt cacggggtgg gccgcaggaa
     1501 ccggcggagc ccgagcgagg cggcgacgag ggccgccccc ccgatgaggt accacaggac
     1561 gaccgagccg gtggcggcga gcacctcatc ggtcgacatc ggcgccgcga tgctgcaggt
     1621 cgaggtcgac gaccgaacgt ccacctggcc gccgaggagc ctggcgaagg cgccccccgc
     1681 gatcgcgtcg accttgtcgg tcccgttgct gccggcgtcc tcggtctcct tcgagaagct
     1741 cccggcgccg aaggtcgtct ccagaggtgt acccacgccg agcgtcacgg gctcgctcgt
     1801 cggcgagatc gccggcaagg cctgccccag caggaacggc gttacggtcg cgcccgatcc
     1861 cttcgcgtcc ggggtcttcc cgtcccaggt gcaggcgacg ttcccctcgc cgatctcgag
     1921 gcggaggaac tcgacgcgct gctcggtcga ggagaacgcg gcgttgcaca cctcacccag
     1981 ctgcgcgttt cgctcggcga ggccgccgca cagcggctcc tggatctgct cggcggcttc
     2041 cttcagggcc accgccgaca gcgacagcgt cacgatgaac ccggtcgacg acgacgccga
     2101 ggtcacgttc tttcccttgg cgttggcgcc tgcggaggtt cccgtcgggg atatctcgac
     2161 cagcgcgccg agcggcgctt cggcgccgcc gccgatcgag agaccaccga acgtggccgt
     2221 gttggtcgac gacggggtgc cgtcctcggc ctcgctcttg gagcgtgctt cgtagctgtg
     2281 tccgctcacc tcgaaggggc cgaccgtctg ggctccttcg gttccggccg ccgttttggc
     2341 cggaccgtcg ggtggggcct tcgtggaaac ggtggcctcg tcgctcccga ggacacccgc
     2401 cgcgaacccc tccgaccgcg gcttcgacgt caccttcgcg cccgtgatgg caccgccgcg
     2461 gtcgacctgc ccctcggcgc ccagcgtgat ccggatgccg ctgccgtcgg agaccgccgt
     2521 gtacgagatc gttcggaa
//
LOCUS       DATLGG010000028         6620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_206803, whole genome shotgun sequence.
ACCESSION   DATLGG010000028 DATLGG010000000
VERSION     DATLGG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206803"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VM638_00685"
     CDS             complement(<1..1050)
                     /locus_tag="VM638_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine-synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HVM10976.1"
                     /translation="MRTSPEVLGRTLAAAPDPDLARLALSRVGEDRAAREALEDHLPA
                     AAALLGFSTAAADFLSRHPGETADVLRADPRTRTQLLAEAEGTVARLGPEAGLRRFRR
                     RALWRLATRDLLGAEVDEVVAEMTGIAEACLEVAAAAVRAPLAIIGMGKLGGAELNYA
                     SDVDVIFVHGDDPERGAAAARRLLALLSAPTADGVALRVDTDLRPEGRAGPLTRSAPA
                     TLEYYRRHAATWERQALLKARSVAGERGLGERLLEDLASVIYPERIDPGAIDDVRGMK
                     VRIEEYVRARGKESTEVKRGRGGIRDVEFAVQLLQMVHGRRDPALRTANTLAALAALG
                     SEGYVAVADAVALADS"
     gene            complement(1047..2360)
                     /locus_tag="VM638_00690"
     CDS             complement(1047..2360)
                     /locus_tag="VM638_00690"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVM10977.1"
                     /translation="MDPAAQRALAEIEGLGARFVRLWFTDVLGFLKSFAIPSEELEKA
                     FREGVGFDGSSIEGFARAEEADMLARPDPATLGVWPGGGQARMFCDITHPDGVAFAGD
                     PREVLRRVLARADGLGYRVLTEPEVEFFLSRSPERFEPLDRGGYFDLAPSEDAERVIA
                     TATSVLEALGIHVALTHHEDAPSQHELGLAPDGALATADALVTLRMVVKEAAQSAGLH
                     ATFMPKPAGGIQGSGLHTHLRLLEGDRNALYDPTGAGGLSKIGRSFVAGLLEHAPAIT
                     AVTNQWVNSYKRLVPGFEAPVYVCWGRRNPSALVRVPADRPDDERACRIEYRAPDPAC
                     NPYLAFALIVAAGLDGVERELEPPPELNEDVRGLDPRELRAAGVRPLPEDLRGALEAM
                     ERSDLVPSVMGEHVFEWFVRNKREEWERARAEVTPAELERSFPLL"
     gene            2471..3166
                     /locus_tag="VM638_00695"
     CDS             2471..3166
                     /locus_tag="VM638_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10978.1"
                     /translation="MRTRRSNGRMAGATLALVLLVAGPAAAHHKSDHSTGSAQAGAPA
                     KQTRGGVTEDNDSDGVRNNIRDAGDNRHPSRKDRSVEHGRSGDQGRSASTPDQDGRGP
                     ERDVGGIDQPDGPGGRDIIDQDRNNGCGNDDDFDDDNEGRCLGPLRTRPSDLRVEDAL
                     DDEEVLDEVIRRPTRGGPVVRGLVITRPAPPDHPRIGALAGTGAPAGTLGVLALALLA
                     GGAALRRSGRREI"
     gene            3213..4541
                     /locus_tag="VM638_00700"
     CDS             3213..4541
                     /locus_tag="VM638_00700"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVM10979.1"
                     /translation="MPPSNDAEFVLRTVEERGIRFIRLWFTDVLGFLKSFAITPHELE
                     TAFAEGMGFDGSSIEGFARIQESDMVAWPDPSTFTLTPWRSEQQVARMFCDIRNPDGT
                     AFHGDPRHVLKRQLDRAAAMGFTFYVGPELEYFYFKDARGTEFLDHGSYFDLTPLDVA
                     TDFRKRTVEYLEAMGIPVEYVHHEVAPSQHEIDLRYTDALTMADNVMTYRLTVKEVAQ
                     EFGVHATFMPKPVRGVNGSGMHVHQSLFEGDRNAFFDAADEYHLSKVGRGYIAGVLHH
                     AREITLVLNQWVNSYKRLVPGYEAPVYICWARRNRSALVRVPMYKPGKEQAARVELRS
                     PDPACNPYLAFAVMLAAGLKGIEEAYDLPPEASNNIYEMAEDERRDAGIDSLPEDLHE
                     AIRLAEGSELLRDTLGEHVLSYLVRNKREEWDEYKSYVTPFELERYLPLL"
     gene            4582..>6620
                     /locus_tag="VM638_00705"
     CDS             4582..>6620
                     /locus_tag="VM638_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVM10980.1"
                     /translation="MSASGIWETLAERLDPGSFRPKLLEDIEVKEFPQRWGNDYAIIA
                     NPRDLTHYQLDVGELEILRRMDGSRTVKELVIERFEGSGDLELSRVTDLVRTLERGNF
                     LERRFIDVYGLIGERLQPTSTFRRKLRQFATTLSVDWQGADRLVRWLYHHGLKWLFRR
                     WALLPGAAFALFGIVAFFILVRSGRFELTGESLALGVVVLLVLDYLMVFVHEIGHASV
                     LVHHGRRVRGAGFQIYFGSPAFFVDSSDGLMLDKKHQIVESLAGPYAQMLMGAVASTI
                     ALLAPDWILSETLYRFAVINYLVLFLNLIPLLELDGYYVLADLIQVPDLRPRSLSFLR
                     FDLWHKIRARERFSAQDVGLGVYGIAGIAFTIFSFYTAYFYWETVFGSLVSKLWAGGT
                     VARVLLVILALFLLAPVARGGARLARTLASRLAAVVRRIRFRLERSWRIEAAELIDDL
                     PLFDDLPGDVLSDLAGRVRLRTVDAGRPVVRQGDRADAFYVVRRGTLVALEEDRRTGA
                     ERTLRTFGRGEAFGELGLVEAAPRRATVRAAEASEVFELDKGTFDRLLVGSVHLPRFE
                     PTLQKLEELRALPSFAHLEHDELAELLQEGAWVDLPAGSDLMVQGAEGDAFYAIGAGQ
                     VEVIRDGARVATLGPGAAVGETALLLGVPRTATVRSLTPVRAFRVEREGFDRLIAR"
BASE COUNT          951 a         2388 c         2329 g          952 t
ORIGIN      
        1 ggagtcggcg agagcgaccg cgtccgcgac ggcgacgtat ccctccgacc ccagcgcggc
       61 cagcgccgcc agcgtgttcg ccgtccgcag cgcgggatcg cgacgtccgt ggaccatctg
      121 caggagctgg acggcgaact cgacgtcgcg gatcccgcca cggccgcgct tgacctcggt
      181 cgactccttc ccccgcgctc gcacgtactc ctcgatccgc accttcatgc cgcgcacgtc
      241 gtcgatcgcc ccgggatcga ttcgctcggg gtagatcacc gaagcgagat cttcgagcag
      301 gcgttcgccg agcccgcgct cccccgcgac cgagcgggcc ttcagcagtg cctgccgctc
      361 ccaggtcgcc gcgtgccgcc ggtagtactc gagcgtggcg ggggcggagc gcgtgagcgg
      421 gcccgcgcgc ccctccgggc ggaggtcggt gtcgacgcgc agggccaccc cgtcggccgt
      481 cggcgcggac agcagggcga ggagtcgccg cgccgcggcc gctccccgct cggggtcgtc
      541 gccgtggacg aagatcacgt cgacgtcgct ggcgtagttg agctccgcgc cgccgagctt
      601 ccccatcccg atgatcgcga ggggcgctcg gacggccgcc gcggcaacct cgaggcaagc
      661 ctcggcgatc cccgtcatct ccgcgacgac ctcgtccacc tcggcaccga ggaggtcgcg
      721 cgtcgcgagc cgccagagcg cccggcgacg gaaccgccgg aggcccgcct ccggcccgag
      781 gcgggcgacg gtcccctccg cctccgcgag gagctgggtc cgcgtccgcg ggtcggcccg
      841 gaggacgtcc gcggtctctc ccggatggcg cgagaggaag tcggcagcgg cggtcgagaa
      901 gccgagcagc gccgcggccg ctggcaggtg gtcttcgagg gcctcgcgcg ccgcccggtc
      961 ctcccccacg cgggacagcg cgaggcgagc gaggtccgga tccggggcgg ccgcgagcgt
     1021 cctcccgagg acctccggcg aggtcctcac agcagcggga aggaccgctc gagctccgcc
     1081 ggggtcacct ccgcacgtgc ccgctcccac tcttcgcgtt tgttgcgaac gaaccactcg
     1141 aagacgtgct cgcccatgac ggatgggacc aggtcggagc gctccatcgc ttcgagcgcg
     1201 ccgcgcaggt cctccggcaa gggccgcacc cccgcagcgc gcagctcgcg cgggtccagc
     1261 ccgcgcacgt cctcgttcag ctcggggggc ggctccagct cccgctccac gccgtcgagg
     1321 cccgcggcga cgatcagcgc gaacgcgagg taggggttgc aggcgggatc gggcgcgcgg
     1381 tactcgatcc ggcaggcccg ctcgtcgtcg ggccgatcgg cggggacgcg cacgagcgcg
     1441 gaggggttgc gcctgcccca gcagacgtag accggcgcct cgaacccggg cacgagccgc
     1501 ttgtaggagt tcacccactg gttcgtcacg gcggtgatcg cgggcgcgtg ctcgagcagg
     1561 ccggcgacga acgagcgtcc gatcttcgac aggccccccg cgcccgtcgg gtcgtagagc
     1621 gcgttgcggt cgccctcgag gagccgcagg tgcgtgtgga ggcccgagcc ctggatgccg
     1681 ccggccggct tcggcatgaa cgtcgcgtgc aaccccgcgg attgcgccgc ctccttcacg
     1741 accatccgca gggtcacgag cgcgtcggcg gtcgcgaggg ccccgtcggg ggcgagcccg
     1801 agctcgtgct gggagggggc gtcctcgtgg tgcgtcagcg cgacgtggat cccgagcgct
     1861 tcgagcacgg aggtcgcggt ggcgatcacc cgctcggcgt cctccgacgg tgcgaggtcg
     1921 aagtagccgc cccggtcgag cggctcgaac cgctccgggg accgggagag gaagaactcc
     1981 acctcgggct cggtcagcac ccgatagccg agtccgtcgg cgcgcgcgag cacccggcgg
     2041 aggacctcgc gcggatcccc ggcgaaggcg acgccgtcgg ggtgggtgat gtcgcagaac
     2101 atccgcgcct ggccgccccc cggccagacg ccgagggtcg cggggtccgg gcgcgcgagc
     2161 atgtcggcct cctcggcgcg ggcgaagccc tcgatcgacg atccgtcgaa gcccacgcct
     2221 tcgcggaacg ccttctccag ctcctccgac gggatcgcga acgacttcag gaaccccagg
     2281 acatcggtga accacagccg gacgaaacgg gcaccgagtc cctcgatctc ggcgagggcg
     2341 cgctgcgcgg cggggtccac gccgtcatcg taccgccgcg accggcggcg gacggacggg
     2401 gcagggaacc ggctcgctac gtcaaatacc gtaatcagtg gctagtgcga accggcggag
     2461 ggcggacagg atgaggacac ggcgatcgaa cgggcggatg gcgggagcca cgctcgccct
     2521 cgtcctcctg gtggcggggc cggccgcggc ccaccacaag tcggaccact cgaccgggag
     2581 cgcgcaggcg ggcgcccctg ccaagcagac gcgcggcggg gtcaccgagg acaacgactc
     2641 cgatggcgtc cgcaacaaca tccgcgacgc cggcgacaac cggcatccgt cgcgcaagga
     2701 tcggtccgtc gagcacggac gctccgggga ccagggccgg tccgcctcga caccggatca
     2761 ggacggccgg ggcccggaac gtgacgtggg aggaatcgac cagccggacg ggcccggggg
     2821 ccgggacatc atcgaccagg accgcaacaa cggctgcggc aacgacgacg acttcgacga
     2881 cgacaacgaa gggcgctgcc tcgggcccct gcgcacccgg ccctccgacc tccgcgtcga
     2941 agacgcgctc gacgacgagg aggtgctcga cgaggtcatc cgccgtccaa cgcgcggcgg
     3001 gcccgtcgtg cggggcctcg tcatcacgcg ccccgctccg cccgaccacc ctcgcatcgg
     3061 cgccctcgcg ggaaccggtg cgcccgccgg aaccctcggc gtgctcgccc tggccctgct
     3121 cgcaggcggc gccgcgctcc gccgcagcgg tcgccgcgag atttaacgcc gacgtaaccg
     3181 cgcggggagg ggcacgccga taccctgggg cgatgccgcc gagcaacgat gccgagttcg
     3241 tcctgcggac ggtggaggaa cgcggcatcc gcttcatccg cctctggttc acggacgtcc
     3301 tcgggttcct gaaatccttc gccatcactc cccacgaact ggagacggcc ttcgccgagg
     3361 gcatggggtt cgacgggtcg tcgatcgaag ggttcgcgcg gatccaggaa tccgacatgg
     3421 tcgcctggcc ggacccctcg acgttcacct tgacgccgtg gcggtcggag cagcaggtcg
     3481 cgcgcatgtt ctgcgacatc cggaaccccg acggcaccgc gttccacggc gacccgcgcc
     3541 acgtcctcaa gcggcagctc gaccgcgccg ccgcgatggg gttcaccttc tacgtcggcc
     3601 ccgagctcga gtacttctac ttcaaggacg cgcgcgggac ggagttcctc gaccacggga
     3661 gctacttcga cctgacgccc ctggacgtcg cgacggactt ccgcaagcgc acggtcgagt
     3721 acctcgaggc gatgggcatc ccggttgagt acgtgcacca cgaggtcgcg ccgtcgcagc
     3781 acgagatcga cctgcgctac acggacgcgc tgacgatggc cgacaacgtc atgacctacc
     3841 ggctcacggt gaaggaggtc gcccaggagt tcggcgtgca cgccacgttc atgccgaagc
     3901 cggtccgcgg cgtgaacggc agcgggatgc acgtccacca gtcgctgttc gagggcgatc
     3961 gcaacgcgtt cttcgacgcc gcggacgagt accacctgtc gaaggtcgga cgcggctaca
     4021 tcgccggggt cctgcaccac gcccgagaga tcacgctcgt gctcaaccag tgggtgaatt
     4081 cgtacaagcg gctggtcccc gggtacgagg cgccggtcta catctgctgg gctcgccgca
     4141 accgctccgc cctcgtccgc gttccgatgt acaagcccgg caaggagcag gccgcccggg
     4201 tcgagctccg ttctcccgac ccggcctgca acccctacct cgcgttcgcc gtcatgctcg
     4261 ccgcggggct gaagggcatc gaggaggcgt acgacctgcc gcccgaggcc tcgaacaaca
     4321 tctacgagat ggccgaggac gagcgccggg acgcggggat cgactcgctg ccggaggacc
     4381 tgcacgaggc gatccgcctc gcggaggggt ccgagctgct ccgcgacacc ctcggcgagc
     4441 acgtcctctc ctacctcgtg cggaacaagc gcgaggagtg ggacgagtac aagtcctacg
     4501 tcacgccgtt cgagctggag cgctacctgc cgctgctctg aggcccttgc catcggaggg
     4561 ccgcgcacgc acgatgttgc ggtgagcgcg agcggcatct gggagacgct cgcagagcgc
     4621 ctcgatcccg ggagcttccg gccgaagctg ctcgaggaca tcgaggtgaa ggagttcccc
     4681 cagcggtggg ggaacgacta cgcgatcatc gcgaaccccc gggacctcac ccactatcag
     4741 ctcgacgtcg gcgagctgga gatccttcgg cggatggacg gctcccgcac ggtgaaggag
     4801 ctcgtcatcg aacgcttcga aggctcgggg gatctcgagc tctcgcgggt caccgacctc
     4861 gtgcgcaccc tggagcgcgg gaacttcctc gagcgtcgct tcatcgacgt ctacgggctg
     4921 atcggcgaac ggctgcaacc cacctcgacc tttcgtcgca agctccggca gttcgccacc
     4981 acgctgagcg tcgactggca gggggccgac cggctcgtgc gttggctcta ccaccacggc
     5041 ctgaagtggc tcttccgacg ctgggcgctc ctgcccggcg cggccttcgc cctcttcggc
     5101 atcgtcgcct tcttcatcct cgtgcgctcg gggcgcttcg agctgaccgg cgaatcgctg
     5161 gcgctcggcg tcgtggtcct gctcgtgctc gactacctga tggtgttcgt ccacgagatc
     5221 ggccacgcgt cggtcctcgt gcatcacggt cgtcgcgtgc gcggcgcggg gttccagatc
     5281 tacttcggct cacccgcctt cttcgtcgac agctccgacg ggctgatgct ggacaagaag
     5341 caccagatcg tggaatcgct cgcgggtccg tacgcgcaga tgctgatggg ggccgtcgcc
     5401 tcgacgatcg cgctcctggc ccccgactgg atcctgtccg agacgctcta tcgcttcgcg
     5461 gtgatcaact acctcgtcct gttcctgaac ctgatcccgc tgctcgagct cgatgggtac
     5521 tacgtcctcg ccgacctgat ccaggtgccc gacctgcggc cgcgatcgct ctcgttcctg
     5581 cgcttcgacc tgtggcacaa gatccgggca cgggaacgct tctcggcgca ggacgtcggg
     5641 ctgggcgtct acgggatcgc ggggatcgcc ttcacgatct tctccttcta cacggcctac
     5701 ttctactggg agacggtgtt cgggagcctg gtctcgaagc tgtgggcggg cgggaccgtc
     5761 gccagggtcc tgctcgtgat cctcgcgctg ttcctgctcg cgcccgtcgc gcggggaggg
     5821 gcgcgcctgg cgcggaccct ggcttcccga ctggcggccg tggtccgccg gatccgtttc
     5881 cgcctcgagc gctcgtggcg gatcgaggca gccgagctga tcgatgacct gccgctgttc
     5941 gacgacctgc ccggcgacgt cctgagcgac ctggcgggcc gcgtccggct ccggaccgtc
     6001 gatgccggcc ggccggtcgt gcggcaaggg gaccgggcgg acgccttcta cgtcgttcga
     6061 cgcggcacgc tcgtggcact cgaggaggac cggcggaccg gagccgaacg caccctccgc
     6121 accttcggcc gtggcgaggc gttcggcgag ctcggcctcg tcgaagccgc accgcgtcgt
     6181 gcgaccgtcc gcgccgccga agcgtcggag gtcttcgagc tcgacaaggg cacgttcgac
     6241 cggctgctcg ttgggtcggt ccacctcccg cggttcgagc cgacccttca gaagctcgag
     6301 gagctgcggg cgcttcccag cttcgcgcac ctcgagcacg acgagctcgc ggagctcctg
     6361 caggaggggg cctgggtgga tctgccggca gggagcgatc tcatggtcca gggagccgag
     6421 ggcgatgcgt tctacgcgat cggcgcgggt caggtcgagg tgatccgaga cggcgcgcgc
     6481 gtggcgacgc tgggtcccgg agccgcggtc ggagagacgg cactgttgct cggggtcccc
     6541 cggacggcta ccgttcgctc gctgacgccg gtccgcgcgt tccgcgtgga gcgtgagggg
     6601 ttcgaccgac tgatcgcccg
//
LOCUS       DATLGG010000029         3470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_81478, whole genome shotgun sequence.
ACCESSION   DATLGG010000029 DATLGG010000000
VERSION     DATLGG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81478"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VM638_00710"
     CDS             complement(<1..929)
                     /locus_tag="VM638_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HVM10981.1"
                     /translation="MNVLGINCFAHDTAACLVQDGRVVAFVEEERFNRDKHTKAFPDA
                     AIAYCLAEGGISIHDVDAVAFAHRAGVDFGRGAADALKRGAPKRLAAQAYVDLNLVRK
                     ERAFRRRWSYTGRSFNVGHHDAHAASAFFASPFEEAAVLTLDRGGDFLSTTLQHGRGN
                     RLRSLAEVRNPDSLGEVYSALTWYLGFHPNSDEGKVMGLAPYGRDRLVPQLRDLLRLT
                     PDGLFRVNLAWFGYQREGRPVSKRFLERYGPPRVPESEITELHEDLAFAVQDLIEEAG
                     LHVTRAIQRMTGSRNLCLAGGVALNSVMNARLL"
     gene            1061..2686
                     /gene="groL"
                     /locus_tag="VM638_00715"
     CDS             1061..2686
                     /gene="groL"
                     /locus_tag="VM638_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133521.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HVM10982.1"
                     /translation="MAGKIIAFDEEARRALERGMDQLANAVRITLGPKGRNVVLEKKW
                     GAPTITNDGVSIAKEIELEDPEEKVGAELVKEVAKKTNDVAGDGTTTATVLAQAMVKE
                     GLRNVAAGANPMSLKRGIEAAVEAAVEAIRNQAKEIEDKDEIAHVAAISAGDAEIGQQ
                     IAEAMDKVGKDGVITVEESQTFGMELDLVEGMRFDKGYISPYFITDPERMEVVLEDAY
                     VLLANQKISAVKDLLPVLEKVMQSGKPLLIIAEDVEGEALATLVVNKIRGTFRSAAVK
                     APGFGDRRKAMLQDIAILTGGQVISEEVGLKLESVGLDMLGQARKVVVTKDETTVVEG
                     AGSEDEIKGRINQIKAEIDKTDSDYDREKLQERLAKLAGGVAVIKVGAATEVELKEKK
                     HRIEDAVSATKAAVEEGIVPGGGVALLGAQQPLEKLDLEGDEATGGRIVFKALEEPVK
                     QIAVNAGLEGGVIVEKIRSENTPGWGLNAATGQYGDMFKAGVIDPAKVTRSALQNAAS
                     IAALFLTTEAVVAEKPEKPQATPPMPGGGDMDF"
     gene            2795..3028
                     /locus_tag="VM638_00720"
     CDS             2795..3028
                     /locus_tag="VM638_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HVM10983.1"
                     /translation="MPRRRLHLTLAGRMRDEPVIASLARGFDLRVNLGRAHIEEDSGW
                     VIVDMDGSDEELERATQWLRTQGVDVEELPGEG"
     gene            3029..3394
                     /locus_tag="VM638_00725"
     CDS             3029..3394
                     /locus_tag="VM638_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM10984.1"
                     /translation="MARKRILLIDHRPEHMRQPVLRLQLDGYDVDTASDGDEGLKMLH
                     EGEYDLLMVDGDLRGADGWDVLKRVREDETLAELKIIVFMAAQGETGKLHLVPVDAEL
                     RRPFPIGELLEKVRKVLGD"
BASE COUNT          605 a         1105 c         1297 g          463 t
ORIGIN      
        1 tgcaggagcc gcgcgttcat cacggaattc agggcgacgc cgccggcgag gcagaggttc
       61 cgcgacccgg tcatgcgctg gatcgctcgc gtgacgtgga gcccggcctc ctcgatcagg
      121 tcctggacgg cgaaggcaag gtcctcgtgg agctccgtga tctccgactc cgggacgcgc
      181 ggtgggccgt accgctcgag gaaccgcttc gacacggggc gaccctcccg ctggtagccg
      241 aaccaggcga ggttcacgcg gaagaggccg tcgggcgtca gacgcaggag atcccgcagc
      301 tgggggacga gccggtcacg gccgtagggc gcgagaccca tcaccttgcc ttcgtcggag
      361 ttcggatgga acccgaggta ccaggtgagc gcggagtaca cctcgccgag cgagtcgggg
      421 ttgcgcacct cggcgagcga ccggagacgg ttgccgcgtc cgtgctgcag ggtggtcgag
      481 aggaagtctc cgccccggtc gagggtcagc acggcggctt cttcgaaggg gctcgcgaag
      541 aaggcgctgg cggcgtgggc gtcgtggtgt ccgacgttga acgaacgccc ggtgtaggac
      601 cagcgccggc ggaaggcgcg ctccttccgg acgaggttga ggtcgacgta ggcctgggcg
      661 gccagtcgct tcggggctcc gcgtttcagc gcgtcggcgg caccgcggcc gaagtcgacg
      721 ccggcacggt gcgcgaaggc caccgcgtcg acgtcgtgga tcgagatccc gccctcggcg
      781 aggcagtacg cgatcgcggc atcggggaag gccttcgtgt gcttgtcccg gttgaaccgc
      841 tcctcctcga cgaacgcgac cacccgcccg tcctggacga ggcaggcggc ggtgtcgtgg
      901 gcgaagcaat tgatcccgag gacgttcacc ggcgtatcct ccccgcccgc gcccgggggg
      961 cgcaacctgc ggttgtcgcc ggcggccggc ccgcgctatc ctcttagcac tctccggggg
     1021 agagtgctaa ggagagccgc gaagcaacga ggagggaagc atggcaggca agatcatcgc
     1081 cttcgacgag gaggcgcgcc gcgcgctcga gcgcgggatg gatcagctcg cgaacgccgt
     1141 tcgcatcacc ctcggcccga agggccgcaa cgtcgtcctc gagaagaagt ggggggcgcc
     1201 gacgatcacg aacgacggcg tgtcgatcgc gaaggagatc gagctcgagg acccggagga
     1261 gaaggtcggg gccgagctcg tcaaggaggt cgcgaagaag accaacgacg tcgccggtga
     1321 cggcacgacg acggccaccg tcctcgccca ggccatggtc aaggagggcc tgcgcaacgt
     1381 cgccgccggt gcgaacccga tgtcgctcaa gcgcgggatc gaggcggccg tcgaggccgc
     1441 ggtcgaggcg atccggaacc aggccaagga gatcgaggac aaggacgaga tcgcgcacgt
     1501 ggcggccatc tccgccggcg acgccgagat cggccagcag atcgccgagg cgatggacaa
     1561 ggtcggcaag gacggcgtga tcacggtcga ggagtcccag accttcggga tggagctcga
     1621 cctcgtcgag gggatgcgct tcgacaaggg ctacatctcg ccgtacttca tcaccgaccc
     1681 ggagcggatg gaggtcgtcc tcgaggacgc ctacgtcctg ctcgcgaacc agaagatcag
     1741 cgcggtgaag gacctcctcc ccgtgctcga gaaggtcatg cagtccggga agccgctcct
     1801 gatcatcgcc gaggacgtcg agggcgaggc gctcgccacc ctcgtcgtca acaagatccg
     1861 cggcacgttt cgctcggctg ccgtcaaggc gcccgggttc ggtgaccgcc gcaaggcgat
     1921 gctgcaggac atcgcgatcc tcaccggggg gcaggtcatc tccgaggagg tcgggctcaa
     1981 gctcgagagc gtcggcctcg acatgctcgg acaggcccgg aaggtcgtcg tgacgaagga
     2041 cgagacgacg gtcgtcgagg gcgcgggctc cgaggacgag atcaagggcc ggatcaacca
     2101 gatcaaggcc gagatcgaca agaccgactc ggactacgac cgcgagaagc tccaggagcg
     2161 tctcgcgaag ctcgctggcg gcgtcgcggt gatcaaggtc ggcgcggcca ccgaggtcga
     2221 gctgaaggag aagaagcacc ggatcgagga cgccgtctcg gcgacgaagg cggccgtcga
     2281 ggagggcatc gtccccggtg gtggggtcgc ccttctcggg gcgcagcagc cgctcgagaa
     2341 gctggacctc gagggcgacg aggcgaccgg cggacggatc gtcttcaagg cgctcgagga
     2401 gccggtgaag cagatcgcgg tgaacgccgg gctcgagggc ggggtgatcg tcgagaagat
     2461 ccgcagcgag aacacgcccg gctggggact gaacgcggcc accggccagt acggcgacat
     2521 gttcaaggcg ggcgtgatcg accccgcgaa ggtcacccgc tccgcgctcc agaacgcggc
     2581 ctcgatcgcg gcgctgttcc tcaccaccga ggcggtcgtc gcggagaagc cggagaagcc
     2641 ccaggcgacg ccaccgatgc ccggcggcgg cgacatggac ttctgagtcg gggcaccacg
     2701 cggaagggcc cggggcgcgt ccccgggccc ttcgtcgttc gggggcttcc gtctcgtggt
     2761 gtcggtccgt gcgggagcat gcgaggatgg gcgcgtgccc agacggcggt tgcacctcac
     2821 cctcgccggc cggatgcgcg acgagccggt catcgcgagc ctcgcccgcg gcttcgacct
     2881 ccgcgtgaac ctgggccgag ctcacatcga ggaggattcg gggtgggtca tcgtggatat
     2941 ggatggctcg gatgaggagc tcgagcgggc gacccagtgg ctccgcacgc agggcgtgga
     3001 cgtcgaggag ctgccggggg aagggtagat ggcgaggaag cggatcctcc tgatcgacca
     3061 ccgtcccgag cacatgcgcc agccggtgct ccggctccag ctggacggct acgacgtcga
     3121 caccgcctcg gacggcgacg aaggcctgaa gatgctccac gagggggagt acgacctgct
     3181 gatggtcgac ggggacctca ggggcgccga cggctgggac gtcctcaagc gggtccgcga
     3241 agacgagacg ctggccgagc tcaagatcat cgtcttcatg gccgcgcagg gggagaccgg
     3301 gaagctgcac ctcgtgcccg tcgacgcgga gcttcggcga ccgttcccga tcggcgagct
     3361 gctcgagaag gtgcggaagg tcctggggga ctgagctcct aggtgccgat caggcgccgt
     3421 gcccagacgt agcgatcgcg gtaccagccg tcgcccaccc agtcgatccc
//
LOCUS       DATLGG010000030         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_223000, whole genome shotgun sequence.
ACCESSION   DATLGG010000030 DATLGG010000000
VERSION     DATLGG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223000"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VM638_00730"
     CDS             complement(<1..216)
                     /locus_tag="VM638_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10985.1"
                     /translation="MLLRRPTAAGAYAAAPLILLAAPSLDHVLGDRLTARRAVEAAFR
                     SDRTVWGHRWALAVSHAGLMVAVATAFP"
     gene            complement(216..659)
                     /locus_tag="VM638_00735"
     CDS             complement(216..659)
                     /locus_tag="VM638_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HVM10986.1"
                     /translation="MEELYKEVILDHYKRPRNKRELPAATVSCHHNNPLCGDDITVHA
                     RLDEGRVAEVAFEGQGCSISQASASMMTEAVAGTAVEEAEALVTDFRSMMEGTTDPDE
                     DEMGDLVALKGVVKYPVRIKCAVLAWDTLQEALRDGRASGSAPHA"
     gene            complement(665..1888)
                     /locus_tag="VM638_00740"
     CDS             complement(665..1888)
                     /locus_tag="VM638_00740"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HVM10987.1"
                     /translation="MSVDVEAIRKDFPILERTVHGRPLVYLDSTATSQKPRAVLDALN
                     DFYERRNANVHRGIYALAEEATAAYEGAREAVARFIGAPSAGSVVFTRGTTEAINLVA
                     HAWGRRFLREGDEIVLTEMEHHSNIVPWQLVAQETGAELRYAGITDDGLLEIDAIQGA
                     MTERTRLVAVTGQSNVLGTITPIQRIADLAHAAGARLLVDGAQMVSHLPVDVGALDVD
                     FLTFSGHKMLGPTGSGGLYGRPELLEEMGPYHGGGEMILEVFHDHSTYKPAPYRFEAG
                     TPPIAQAVGLGAAVEYLSAIGMEAIREHERDLTAYALERLEGVGARVFGPTDLDRRGG
                     AISFWIDGVHPHDLATLVDREGVAIRAGHHCAQILMRRLGVPATARASFYLYNLREEV
                     DALVEALERAKGVFA"
     gene            complement(1885..2661)
                     /gene="sufC"
                     /locus_tag="VM638_00745"
     CDS             complement(1885..2661)
                     /gene="sufC"
                     /locus_tag="VM638_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVM10988.1"
                     /translation="MTNILEIDDLHVSVEGKEILRGVTLSVPQGEVHALMGPNGSGKS
                     TLANVVMGRPGYEVTGGDIRYRGTSITELTPDERAQAGLFLAFQYPVEVPGVSVVNFL
                     RAAYNASKQQEMSALAFRKRLKEKMALVGVDDDMVRRYVNQGFSGGEKKRMETLQLAV
                     LEPEIAILDETDSGLDIDALKAVATGIDDLRGDTLGILLITHYQRILDYIAPDRVHVL
                     MAGRVVKSGGPELARELEAKGYEGIRAELGIGVEGEPARA"
     gene            complement(2658..2972)
                     /locus_tag="VM638_00750"
     CDS             complement(2658..2972)
                     /locus_tag="VM638_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HVM10989.1"
                     /translation="MTLTTLGRVGDVPEGEVRRFDVGGHAIAVVNLGGGEFRAVGDVC
                     SHAEAYLHEGDVDVEDRTVECPRHGSVFDLDSGAPKTLPATVPVPTYMLTIEDDEIKV
                     EL"
     gene            complement(2969..>4173)
                     /gene="sufD"
                     /locus_tag="VM638_00755"
     CDS             complement(2969..>4173)
                     /gene="sufD"
                     /locus_tag="VM638_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HVM10990.1"
                     /translation="ALPMPSQETEEWRYTDLSGLELEAYETFAPARRGSTLDDLDEGL
                     LEAAGAIGDRGGLAVQHNSETAMVHLDPDLAGKGVVFTGLDEAIEAHGEMLEKRLHRL
                     VPPERSRLVAQHAAYRTGGMFLYVPAGVTVELPLQSLTYLDADRTAVFPHTVVVVEED
                     AEVTLIERFVSSRLDDAVSNAVTEIFAAPNARVRHISLQDWGSGVRHFGVQRAQLERD
                     AQLRSLVVSFGGSLSRTEAESIMAAPGAYSEMLGVYFADGDQHFDFRSLQDHAAPHCT
                     SDLLYKGALRDRSRAVYSGWVIVRPGAQKTDAFQTNRNIVLSDAAKADSIPNLEIEAN
                     DVRCGHAASVGPVERDEVFYLQTRGIPEAEAERLIVTGFFQEVLDRVGIDEVRAGLEE
                     AIQVELRR"
BASE COUNT          610 a         1463 c         1465 g          635 t
ORIGIN      
        1 ggggaacgcc gtcgcgacgg cgaccatcag accggcatgc gacacggcga gcgcccagcg
       61 atgaccccag acggttcggt ccgagcggaa cgcggcctcc accgcgcgcc gagccgtgag
      121 ccggtccccc agcacatggt cgagcgacgg agcggcgagg aggatcagcg gcgcggcggc
      181 gtaggccccg gccgcggtcg ggcggcggag cagcatcagg cgtgcggggc cgagccgctc
      241 gcccggccgt cgcgcagcgc ttcctggagc gtgtcccagg cgaggaccgc gcacttgatc
      301 cgcaccgggt acttcacgac gcccttgagc gcgacgaggt cgcccatctc gtcctcgtcg
      361 ggatcggtcg tgccctccat catcgagcgg aagtcggtga cgagcgcctc ggcctcttcc
      421 acggcggtgc ccgcgaccgc ctcggtcatc atcgaggcgg aggcctgcga gatggagcag
      481 ccctggccct cgaacgcgac ctctgcgacg cgcccctcgt cgaggcgagc gtgcacggtg
      541 atgtcgtccc cgcagagcgg gttgttgtga tggcaggaga ccgtcgccgc ggggagctca
      601 cgcttgttcc gcggtcgctt gtagtgatcg aggatcactt ccttgtacag ctcctccacc
      661 gccactacgc gaacaccccc ttcgcccgct cgagcgcctc caccagcgcg tccacctcct
      721 cgcggaggtt atacaggtag aacgaggcgc gcgcggtggc gggcacgccc agccgccgca
      781 tcaggatctg cgcgcagtga tggcccgcgc ggatcgcgac gccctcgcgg tcgacgaggg
      841 tcgccaggtc gtgggggtgc accccgtcga tccagaagga gatcgcgccc cctcgccggt
      901 ccaggtcggt cgggccgaag acccgcgcgc cgacgccttc caggcgctcg agcgcgtagg
      961 ccgtgaggtc gcgttcgtgc tcgcggatcg cctccatccc gatcgcggag aggtactcga
     1021 cggcggcgcc gaggcccacc gcctgagcga tgggcggcgt ccccgcctcg aaccggtagg
     1081 gcgcgggctt gtaggtcgag tggtcgtgga agacctcgag gatcatctcg cccccgccgt
     1141 ggtagggccc catctcctcg agcagctccg gccggccgta gagcccgcct gagcccgtcg
     1201 gacccagcat cttgtggccc gagaacgtca ggaagtccac gtcgagcgct ccgacgtcga
     1261 ccgggaggtg cgaaaccatc tgcgcgccgt cgaccaggag cctcgccccg gccgcgtggg
     1321 cgaggtcggc gatccgctgg atcggggtga tcgttccgag tacgttcgac tggccggtca
     1381 ccgcgaccag acgggtccgc tccgtcatcg caccctggat cgcgtcgatc tccaggaggc
     1441 cgtcgtcggt gattccggcg tagcgcagct cggcgccggt ctcctgggcg acgagctgcc
     1501 acgggacgat gttcgagtgg tgctccatct cggtgaggac gatctcgtca ccctcgcgga
     1561 ggaagcgtct gccccaagcg tgcgcgacga ggttgatcgc ctccgtcgtc ccgcgcgtga
     1621 acacgacgct ccccgcggag ggcgcgccga tgaagcgggc gacggcttct cgcgcgccct
     1681 cgtaggcggc agtggcctcc tccgcgagcg cgtagatgcc gcgatgcacg ttcgcgttgc
     1741 gccgctcgta gaagtcgttg agcgcatcga ggacggcgcg cggcttctgg gaagtggccg
     1801 tggaatcgag gtagaccagc ggccgcccgt gaacggtccg ctcgaggatc gggaagtcct
     1861 tgcggatcgc ctcgacgtcc acgctcacgc cctcgccggc tcgccctcga cgccgatccc
     1921 gagctcggcc cggatcccct cgtacccctt cgcctcgagc tcgcgggcaa gctcggggcc
     1981 gccggacttc accacgcggc ccgccatcag gacgtggacg cggtcgggag cgatgtagtc
     2041 gaggatccgc tggtagtgcg tgatcagcag gatgccaagg gtgtcgccgc ggaggtcgtc
     2101 gatcccggtc gcgacggcct tcagcgcatc gatgtcgagc cccgagtcgg tctcgtcgag
     2161 gatcgcgatc tccggctcga gcacggcgag ctggagcgtc tccatccgct tcttctcgcc
     2221 accggagaac ccctggttca cgtagcgccg gaccatgtcg tcgtcgacgc cgacgagcgc
     2281 catcttctcc ttgagacgct tgcggaacgc gagcgccgac atctcctgct gcttcgaggc
     2341 gttgtaggcg gcacgcagga agttcacgac cgagacgccg ggcacctcga ccggatactg
     2401 gaacgcgagg aacaggccgg cctgggcccg ctcgtcgggc gtcagctccg tgatggaggt
     2461 cccccggtag cggatgtcgc cgccggtcac ctcgtagccg ggccgcccca tcacgacgtt
     2521 cgcgagggtc gacttcccgg agccgttcgg ccccatgagc gcgtgcacct ccccctgggg
     2581 gacggagagg gtcacgccgc ggaggatctc cttgccttcc acggacacgt gcaggtcatc
     2641 gatctccagg atgttcgtca tagctccacc ttgatctcgt cgtcttcgat cgtgagcatg
     2701 taggtcggca ccgggacggt ggccgggagg gtcttcggcg cgccgctgtc gaggtcgaac
     2761 acggagccgt gacgcgggca ctcgaccgtg cggtcctcga cgtcgacgtc gccctcgtgc
     2821 agataggcct ccgcgtgcga gcagacatcg ccgacggcgc ggaactcgcc cccgccgagg
     2881 ttcacgacgg cgatcgcgtg accgcccacg tcgaagcggc gaacctcgcc ctccgggacg
     2941 tcgccgacgc gaccgagcgt ggtcagggtc atcgccgcag ctccacctgg atcgcctcct
     3001 cgagcccggc gcggacctcg tcgatcccca cgcggtcgag gacctcctgg aagaacccgg
     3061 tgacgatcag gcgctccgcc tcggcttccg ggatgccgcg cgtctggagg tagaagacct
     3121 cgtcccgctc gaccggcccg acgctcgccg cgtgcccgca gcgcacgtcg ttcgcctcga
     3181 tctcgaggtt ggggatcgag tccgccttcg ccgcgtcgga caggacgatg ttgcggttcg
     3241 tctggaaggc atccgtcttc tgcgccccag gacggacgat cacccagccg gagtaaacgg
     3301 cgcgggagcg gtcgcgcagc gcgcccttgt acaacaggtc gctcgtgcag tgcggagcgg
     3361 cgtgatcctg gagggagcgg aagtcgaagt gctggtcacc gtccgcgaag tagacgccga
     3421 gcatctcgga gtaggccccg ggcgccgcca tgatgctctc ggcctcggtg cgggacagcg
     3481 agccgccgaa ggagacgacg agggagcgca gctgcgcgtc gcgctcgagc tgtgcgcgct
     3541 gcacgccgaa gtggcgaacc ccgctccccc agtcttggag cgagatgtgc cgaacccgcg
     3601 cgttcggcgc ggcgaagatc tcggtcacgg cgttggagac ggcgtcatcg agtcgagatg
     3661 agacgaagcg ctcgatcagc gtcacctccg cgtcttcctc gacgaccacg accgtgtgcg
     3721 ggaagacggc ggtgcgatcg gcgtcgaggt aggtcaggct ctggagcggg agctccaccg
     3781 tcaccccggc ggggacgtac aggaacatcc cgcccgtgcg ataggcggca tgctgcgcga
     3841 cgaggcggct gcgctccggc ggcaccagcc ggtggaggcg cttctcgagc atctcgccgt
     3901 gggcctcgat ggcctcgtcg agcccggtga agaccacgcc cttccctgcg aggtccgggt
     3961 cgaggtgaac catcgcggtc tcggagttgt gctgcacggc gaggccaccg cgatcgccga
     4021 tcgctccagc cgcctcgagc aggccctcat cgagatcgtc gagcgtcgag ccgcggcggg
     4081 cgggggcgaa cgtctcgtac gcttcgagct cgagccccga gaggtcggtg tagcgccact
     4141 cctcggtctc ctgcgacggc atcgggagcg cct
//
LOCUS       DATLGG010000031         3256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_143882, whole genome shotgun sequence.
ACCESSION   DATLGG010000031 DATLGG010000000
VERSION     DATLGG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143882"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VM638_00760"
     CDS             <1..440
                     /locus_tag="VM638_00760"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HVM10991.1"
                     /translation="VFLISDGDVAGAIVAAAPERQAADMLMGIGGTPEGVVAAAALKC
                     MGGAMQGKLYPRNDEERETLVGTGFDLDQVITIDDLVAGDDAFFAATGVTNGALLRGV
                     RYRTDHVITQSMVMRAKSGTVRYIEGHHQLDKLMRFSQIDYRS"
     gene            complement(437..661)
                     /locus_tag="VM638_00765"
     CDS             complement(437..661)
                     /locus_tag="VM638_00765"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HVM10992.1"
                     /translation="MSDGVLEAALRVLGEEGEPLHWTKIQDLALRRGYLDPFATPDVR
                     KRIVAALAGAARRTDGPVERVSTGVYALRR"
     gene            complement(658..1659)
                     /locus_tag="VM638_00770"
     CDS             complement(658..1659)
                     /locus_tag="VM638_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM10993.1"
                     /translation="MRIGLALPHYDFSSPEGGPVTWDGLAAAARRAEALGFDSVWVSD
                     HFFLSLARYGGPDEPFGTVEPLSALAGLAVATERVRLGTLVLGAGFRHPAILAKAATS
                     IDLLSGGRLDLGIGAGWYEDEYRAFGYGFGTAGERFEILEETLEVLRRLFAEGPSTWE
                     GQRFRLEEAFNRPEPVQEGGPPIWVGGKGGPRLRRLVATHADGWNTVWAWTPEAYGER
                     VAALREACERAGREMVRLSLGLYTLVGEDERDLVSRYRSLQEWTPGGALDGALIEEWG
                     RDKLVGTPEQVLERLATFGAIGVEEVIVCAASIPFAVHDVSTLDVIAETVIPRARDL"
     gene            complement(1667..3016)
                     /locus_tag="VM638_00775"
     CDS             complement(1667..3016)
                     /locus_tag="VM638_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HVM10994.1"
                     /translation="MAAVSDIVGIGLAGGQGVRARPLTLKAPGYLRSKAAMAFLGQRL
                     IRWVVMILSAEGVKEYWVVAHGKENRYQIKVLLGYGEALGVDVQYSPVKFDALSTGSA
                     DSTLRMIEHWGLERPALVFPTDSIIDFDLEPMYRQHLETGAAVTIAAMVRAPAEVAEK
                     YGVMLTGSERRIHEFVEKPSLGALREHFQVPDPAEFDRLPLLTNAGFYLLDPAAMRRA
                     IADPEVAAMRRERLDFGKDLLPWLVDRGYPVHAFPVRRLGDLGNVEDYVETAVEVLHG
                     RFESVARLMGPPYDPEANVWIAPESLHLRDAASGKTLAEKMAEGLVTVGPAVRIGRFC
                     EIGPGVVIVESNLDDDVEVREGAYVARSTIRDGAMVGPASIVRNSVVGSMSEIRSEVG
                     RPTVIEDYVALGDEAVVHAGVRLASGVSVYPRLKVPAGVRVAPGTELTGPQDILDSL"
     gene            complement(3024..>3256)
                     /locus_tag="VM638_00780"
     CDS             complement(3024..>3256)
                     /locus_tag="VM638_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM10995.1"
                     /translation="AGGLDADRGPYRAVLEGGGRRRIGRLEPSMPGELAVYRYFRRDV
                     SAARSVAVRDAAGRAVLRGTVVAEQAPRGADV"
BASE COUNT          489 a         1221 c         1127 g          419 t
ORIGIN      
        1 gcgtcttcct catctccgac ggggacgtcg ccggggcgat cgtcgcggcg gcgccggagc
       61 ggcaggcggc ggacatgctc atgggcatcg gggggacgcc ggagggggtc gtcgcggcgg
      121 cggcgctgaa gtgcatgggc ggagcgatgc agggcaagct ctaccctcgc aacgacgagg
      181 agcgcgagac gctggtcgga accggcttcg accttgacca ggtcatcacg atcgacgacc
      241 tcgtcgccgg cgacgacgcc ttcttcgccg cgacgggcgt gacgaacggc gcgctcctcc
      301 gcggggtccg ctaccgcacg gaccacgtga tcacgcaatc gatggtgatg cgcgccaagt
      361 cggggaccgt ccgctacatc gaggggcacc accagctcga caagctgatg cggttctcgc
      421 agatcgacta ccgctcctag cggcggaggg cgtagacgcc ggtcgacacg cgctcgacgg
      481 gaccgtcggt ccgccgcgcc gctcccgcca gcgccgcgac gatccgcttg cggacgtcgg
      541 gcgtcgcgaa cgggtcgagg tagccgcgcc ggagcgcgag gtcctggatc ttcgtccagt
      601 gcaggggctc gccctcctcg ccgaggacgc gcagcgcggc ctcgaggacc ccgtcgctca
      661 caggtcccgc gcgcgaggga tgacggtctc cgcgatcacg tcgagggtgc tcacgtcgtg
      721 gaccgcgaac gggatcgacg cggcgcagac gatgacctcc tcgaccccga tcgccccgaa
      781 cgtggcgagc cgctcgagca cctgctcggg ggtccccacg agcttgtccc gcccccactc
      841 ctcgatcagc gcgccgtcga gcgcgcctcc cggggtccac tcctgcagcg accgatagcg
      901 ggagacgagg tcgcgctcgt cctcaccgac gagcgtgtac agcccgaggg agaggcggac
      961 catctcgcgc ccggcccgct cgcacgcctc gcgcagcgcg gcgacgcgct ccccgtaggc
     1021 ctccggcgtc cacgcccaca cggtgttcca gccgtcggca tgcgtcgcga cgagccgccg
     1081 caggcggggc ccccccttgc cgccgaccca gatcggcggc ccgccttcct ggacgggttc
     1141 cggacggttg aacgcctcct cgagccggaa gcgctgtccc tcccaggtcg acgggccctc
     1201 cgcgaacagg cgccgcagca cctcgagcgt ctcttcgagg atctcgaacc gctcccccgc
     1261 ggtcccgaac ccgtagccga aggcgcggta ctcgtcctcg taccagccgg cgccgatgcc
     1321 gaggtccagc cgtccgcccg acagcaggtc gatcgaggtc gcggccttcg cgaggatcgc
     1381 cgggtgccgg aaccccgccc cgagcacgag cgtgccgagg cgaacccgct ccgtcgcgac
     1441 ggccagaccg gccagcgcgc tcaggggctc gaccgtgccg aacggctcgt cgggtccgcc
     1501 gtaacgcgcc agtgacagga agaagtggtc cgagacccag accgagtcga agccgagcgc
     1561 ctcggcccgt cgcgccgcag ccgccaggcc gtcccaggtc acgggacccc cctcggggga
     1621 ggagaagtcg tagtgcggca acgccagccc gatcctcatc gcgagctcac agggagtcga
     1681 ggatgtcctg gggccccgtc agctcggtcc cgggcgcgac gcggacgccg gcgggaacct
     1741 tcagccgcgg gtacacgctg accccggacg cgagccggac cccggcgtgg acgaccgctt
     1801 cgtccccgag cgcgacgtag tcctcgatca cggtcggtcg tccgacctcg gagcggatct
     1861 cgctcatgga tccgacaacg gagttccgca cgatcgaggc cggcccgacc atcgccccgt
     1921 cgcggatcgt cgagcgggcg acgtaggcgc cctcgcggac ctcgacgtcg tcgtcgaggt
     1981 tcgactcgac gatgacgacg ccggggccga tctcgcagaa ccgcccgatg cgcaccgcgg
     2041 gaccgacggt gacgagcccc tcggccatct tctccgcgag ggtcttgccg ctcgcggcat
     2101 ccctcaggtg cagggactct ggggcgatcc acacgttcgc ctcggggtcg tacggcgggc
     2161 ccatcaaacg cgcgacggac tcgaagcgcc cgtgcagcac ctcgaccgcg gtctcgacgt
     2221 agtcctcgac gttgccgagg tcgccgagac ggcgcacggg gaacgcgtgg accgggtagc
     2281 cgcggtcgac gagccacggc aggaggtcct tgccgaagtc gagccgctcc cgccgcatcg
     2341 ccgcaacctc cgggtcggcg atcgcccgcc gcatcgccgc ggggtcgagc aggtagaacc
     2401 cggcgttcgt caggaggggc aggcggtcga actccgccgg atcggggacc tggaagtgct
     2461 cccggagagc ccccagggag ggcttctcga cgaactcgtg gatccgtcgc tcgctcccgg
     2521 tcagcatcac gccgtacttc tccgcgacct ccgcgggagc ccgcaccatc gcggcgatcg
     2581 tcaccgccgc ccccgtctcg aggtgctgcc ggtacatcgg ctcgagatcg aagtcgatga
     2641 tcgagtcggt cgggaacacg agggccggcc gctcgaggcc ccagtgctcg atcatccgaa
     2701 gggtggaatc ggcgctgccg gtcgacagcg cgtcgaactt caccggcgag tactgaacgt
     2761 cgacgccgag cgcttctccg tagccgagga ggaccttgat ctggtagcgg ttctccttgc
     2821 cgtgggcgac gacccagtat tccttcacgc cctcggccga gaggatcatc acgacccagc
     2881 ggatgagccg ctgaccgagg aacgccatcg cggccttcga gcgcaggtac ccgggcgcct
     2941 tgagcgtcag cggtcgcgca cggacaccct gcccgccggc gagcccgatg cccacgatgt
     3001 cggagacggc ggccacgcga gcatcatacg tccgcccctc gaggagcttg ctccgcgacg
     3061 accgtgccgc gcagcacggc ccggccggcg gcatcgcgca ccgcgaccga acgcgccgcc
     3121 gagacgtctc ggcggaagta ccggtagacc gcgagctcgc ccggcatcga cggctcgagc
     3181 cgcccgatcc gccggcgccc cccgccctcg aggaccgccc ggtaggggcc ccggtcggcg
     3241 tcgagccccc cggcta
//
LOCUS       DATLGG010000032         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_81641, whole genome shotgun sequence.
ACCESSION   DATLGG010000032 DATLGG010000000
VERSION     DATLGG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81641"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VM638_00785"
     CDS             complement(<1..1540)
                     /locus_tag="VM638_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVM10996.1"
                     /translation="MTEAAPRAARLPRRGARAPVGVVAAAIPVCALLALPVAYLAIRA
                     AGAEASSWDLILRPRTVWLAARTAGLTLAVTTGAIALGVPMAWLTTRTDLPFRRAWAV
                     LGALPLVIPSYVGAFTLLSFLGPRGLLRQILGVERLPDISGFPGALLALTLFTFPYVY
                     LTTAAAIRGLDPALEDAARGLGHSRWTTFRRVTLPLLRPSIGAGALLAALYTLHDFGA
                     VSLMRFPVFTQAIYLQYRAAFDRTPAAILSLFLVAMALVVVWLERRARGRALLYRTEA
                     GTPRPPSRIALGRWRGPALAFCTLVVLTSLVLPVGVLVYWLVRGIQAGQEIGAPWRAM
                     LSSMSASGGGAVLAVTAALPIAILAARHRGLAEAPARLAYAAFALPGIVVALAFVFFA
                     ANVTPALYQTLPLLVLAYVVLFLPQAGEPLRGSLLQINERIEEAGRSLGHGRFAVFRR
                     LTLPLVARGALAGFGLVFLTAMKELPATLLLRPTGFETLATRVWTHASVAQYARAAAP
                     ALLLL"
     gene            complement(1545..>2426)
                     /locus_tag="VM638_00790"
     CDS             complement(1545..>2426)
                     /locus_tag="VM638_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HVM10997.1"
                     /translation="LLARFEEQNGIALEVRYGDSAELAATILEEGQNSPADVFISQDA
                     GSLGSVADEGLFTEIDQGILDRVDERFRSAEGLWVGVSGRARVAAYNTERLQEDDLPA
                     SIMDFTDPEWRGRIGFPPTNASFQAFVAAMLITEGEETTREWLEGIVANEPRQYEGNS
                     EVVQGIADGEIDVGFVNHYYKYELEAEEGSELPVANHFFSGGDPGALVNVAGAATLAT
                     TDRPEEASRLIDFLTGPEGQEYFATETWEYPVIDGVDPQAELPSLEEIESPELDLSDL
                     GGTLEPALRLLADVGLV"
BASE COUNT          389 a          850 c          907 g          280 t
ORIGIN      
        1 ccagcagcag gagggccggg gcggccgccc gagcgtactg agcgaccgag gcgtgcgtcc
       61 agacgcgcgt ggcgagcgtc tcgaaccccg tcggccgcag caggagcgtg gcgggcagct
      121 ccttcatcgc ggtcaggaac acgagcccga agccggccag cgctccccgg gcgacgagcg
      181 gcagcgtcag gcgacggaag acggcgaacc tcccgtggcc gagggacctc ccggcctctt
      241 cgatcctctc gttgatctgg agcagggagc cgcgcagggg ttcgcccgcc tgcggaagga
      301 agagcacgac gtaggcgagc acgaggagcg gcagggtctg gtagagcgcc ggcgtcacgt
      361 tcgccgcgaa gaacacgaac gccaacgcca cgacgatccc cggcagcgcg aaggcggcgt
      421 aggcgaggcg cgcgggcgcc tcggcgagcc cacggtgccg cgcggcgagg atcgcgatcg
      481 gcagagcggc cgtgacggcc agcaccgccc cgccgcccga agccgacatc gaagagagca
      541 tcgctcgcca cggagcgccg atctcctgac ccgcctggat ccctcggacg agccagtaga
      601 cgagcacccc gaccggcagg accagcgacg tcagcaccac cagggtgcag aacgccagcg
      661 ccggtcctcg ccatcggccg agcgcgatcc gcgacggtgg acgtggtgtc ccggcctcgg
      721 tccggtacag gagcgccctt ccccgggccc ggcgctcgag ccagacgacg acgagggcca
      781 tggccacgag gaacagcgag aggatcgcgg ccggcgtccg gtcgaacgcc gcccggtact
      841 ggaggtagat cgcctgcgtg aacaccggga agcgcatcag cgagaccgcg ccgaagtcgt
      901 ggagcgtgta gagggccgcg aggagcgcgc ccgccccgat cgagggccgc agcaagggca
      961 gcgtcacgcg ccggaaggtc gtccaccggg aatggccgag ccctcgggcg gcgtcctcga
     1021 gggccgggtc gagcccgcgg atcgccgcgg cggtcgtcag gtagacgtag gggaacgtga
     1081 acagggtgag ggcgaggagc gccccgggga agccgctgat gtccggaagg cgttcgactc
     1141 cgaggatctg ccggaggagg ccgcggggcc cgaggaagga caggagcgtg aacgctccga
     1201 cgtaggacgg gatgacgagg gggagcgcgc cgaggaccgc ccaggcgcgc cggaagggca
     1261 gatcggtcct cgtggtgagc caggccatcg ggaccccgag ggcgatcgca cccgtcgtga
     1321 ccgccagcgt gagcccggcg gtccgcgccg cgagccagac ggtccgtggc cgcaggatca
     1381 ggtcccagct cgaggcttcg gctcccgccg cccggatcgc gaggtaggcg accggcaggg
     1441 cgagcagggc gcagaccggg atggcggccg ccaccacccc caccggcgcc cgcgcgccgc
     1501 gccgcgggag gcgggcggcg cgcggggcgg cctcggtcat ccgctcacac gagccccacg
     1561 tcggcgagca ggcggagagc cggctcgagc gtgccgccga ggtcggagag gtccagctcg
     1621 ggcgactcga tctcctcgag cgagggaagc tccgcctggg gatccacccc gtcgatcacc
     1681 gggtactccc aggtctcggt cgcgaagtac tcctggccct ccggcccggt gaggaagtcg
     1741 atcagccggc tcgcctcctc cggtcggtcc gtcgtcgcga gggtcgcggc gccggcgacg
     1801 ttgaccagcg cgccggggtc gccgccggag aagaagtggt tcgcgaccgg cagctccgag
     1861 ccctcctcgg cctcgagctc gtacttgtag tagtggttca cgaagccgac gtcgatctca
     1921 ccgtcggcga tgccctgaac cacctcggag ttgccctcgt actggcgcgg ctcgttcgcc
     1981 acgatcccct cgagccactc gcgcgtcgtc tcctctccct ccgtgatcag catcgccgcc
     2041 acgaaggcct ggaaggaggc attggtgggc gggaatccga tccgtccccg ccactcgggg
     2101 tcggtgaagt ccatgatcga ggcgggcagg tcgtcctcct gcaggcgctc ggtgttgtag
     2161 gcggcgacgc gcgcgcgccc ggacacgccc acccagagcc cctccgccga gcggaaccgc
     2221 tcatccaccc ggtcgaggat cccttggtcg atctcggtga agaggccctc gtcggcgacg
     2281 gagccgaggg agccggcgtc ctgagagatg aacacgtcgg cgggggagtt ctgcccctct
     2341 tccaggatcg tggccgccag ttcggcggaa tcgccgtagc ggacctcgag agcgatcccg
     2401 ttctgctcct cgaaccgcgc gaggag
//
LOCUS       DATLGG010000033         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_97106, whole genome shotgun sequence.
ACCESSION   DATLGG010000033 DATLGG010000000
VERSION     DATLGG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97106"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1131)
                     /locus_tag="VM638_00795"
     CDS             complement(52..1131)
                     /locus_tag="VM638_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HVM10998.1"
                     /translation="MAHRVTFIPGDGVGPELSEATRRVLEATGVQFDWEEQQAGADIM
                     EEHGTPLPDHVLESIRRNGVAIKGPITTPVGTGFRSVNVALRKELDLFACLRPCKTYP
                     GVRSRYEGVDVVIVRENHEDLYAGVEFEQGSADAKQLIELVRELTGKQIREDSGISIK
                     PISVTGTRRIVQWAFDYARREGRRKVTAVHKANIMKFSDGLFLQTAREVAEENSDIEF
                     EDRIVDNLAMQLVSRPEEYDVLVLPNLYGDIISDLGAGLIGGLGVAPGANIGDTAAVF
                     EATHGSAPKYKGMNKVNPMAMMLSGVLMLRHLDEREAADRLEAAIASVIEEGKSVTYD
                     MKPSRDDPTAVGTSEVADAVIERLG"
     gene            1243..2361
                     /locus_tag="VM638_00800"
     CDS             1243..2361
                     /locus_tag="VM638_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HVM10999.1"
                     /translation="MGEKGLADVVAAETKLSDIDGKLGKLFYVGYDIHDLAQHATFEE
                     TMYLLHYLRLPNRDELDDLNERLLAERDLDEFTAGLMPTFAEAASPMSMLRTGVSAGS
                     AYDPDGWDNSAEANERKAIRLMARFPTMIAAYHRLRTGQDPIPPNPKLPHAANFLTML
                     LGEEPDEEVARAFDICLVLHADHTMNASTFAGRVVASTLADMHSAITAAIAALKGPLH
                     GGANEAVMKMVEEIGDVGRAEAYVKAKLGRKEKIMGFGHRVYKTEDPRATHLRRLSRE
                     LGQRAGDTRWYEISERIEKVVMDEKGLYPNVDFYAASVYRSMGIPTDLFTTVFAASRV
                     SGWTAHVREQLADNRLIRPESDYIGPTGLRYVPIEERG"
     gene            2358..2819
                     /locus_tag="VM638_00805"
     CDS             2358..2819
                     /locus_tag="VM638_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVM11000.1"
                     /translation="MKIRDIMTEAAVTDQPDDTLASAAAKMWEQQTGSLLVTQQDELV
                     GIVTERDVLKAVATGTPLDAPLGTVMSKDLVTVGPGTSLREAAKIMTERWIRHLPVLD
                     GGRLVGIVSQRDLSGVLAGALNEPEGLHRLIESSELVRERRLARIEAGDLD"
     gene            complement(2816..>3707)
                     /locus_tag="VM638_00810"
     CDS             complement(2816..>3707)
                     /locus_tag="VM638_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVM11001.1"
                     /translation="LSRMRLAESGGGRAFGMDARIRAADGSTTAVFLTGLLVSDDEDR
                     PLCFVVQIHDFQARRTAREELERTLDELRRADEQKSRFVAVAAHELRSPLTSVLGFAK
                     TLSRHWEVIAEDERRQHLGLVVEQAERLSRLVGDLLTISRIEGGSVRMQKQTLDALQE
                     VGSIVAELPCAEDVDVVGDEGLKLEADPDHLRQMLTNLVTNAYKYGETPIEVEIRDEG
                     DSIEILVRDEGPGVPREFVPQLFESFSRAETANTRGSGGTGLGLSIVKGLAKAHGGDA
                     WYEPNHPTGACFGIRLPVSA"
BASE COUNT          559 a         1354 c         1240 g          554 t
ORIGIN      
        1 ctcccgacca ccgtcacctt catcgcgtcc gtcctttcgc ctgagggatc ctcagccgag
       61 cctctcgatc acggcgtcgg cgacctcgga ggtcccgacg gccgtggggt cgtcgcggct
      121 cggcttcatg tcgtaggtca cgctcttgcc ctcctcgatc accgaggcga tcgcggcctc
      181 cagccggtcg gccgcctcgc gctcgtcgag gtggcggagc atcagcacgc ccgacagcat
      241 catcgccatc gggttcacct tgttcatgcc cttgtacttc ggggcgctgc cgtgggtcgc
      301 ctcgaaaacc gccgcggtat cgccgatgtt cgcccccggc gccacgccga gtccgccgat
      361 caacccggcg ccgaggtccg agatgatgtc gccgtagagg ttcgggagca cgagcacgtc
      421 gtactcctcc ggccgcgaca cgagctgcat cgcgaggttg tcgacgatcc ggtcctcgaa
      481 ctcgatgtcg ctgttctcct ccgccacctc gcgcgccgtc tggaggaaga gcccgtccga
      541 gaacttcatg atgttcgcct tgtggaccgc ggtgaccttg cggcggccct cgcggcgggc
      601 gtagtcgaac gcccactgca cgatccggcg cgtccccgtg accgagatcg gcttgatcga
      661 gatgccggag tcctcgcgga tctgcttccc ggtcagctcg cggacgagct cgatgagctg
      721 cttcgcgtcg gcgctcccct gctcgaactc gacgccggcg tacaggtcct cgtggttctc
      781 gcgcacgatc accacgtcga cgccctcgta gcggctccgc acgcccgggt aggtcttgca
      841 cgggcgcagg caggcgaaga ggtcgagctc cttccgaagg gcgacgttca cgctgcggaa
      901 gccggtcccg accggggtcg tgatcgggcc cttgatcgcg acgccgttcc gccggatcga
      961 ctccagcacg tggtccggca gcggcgtccc gtgctcctcc atgatgtcgg cgccggcctg
     1021 ctgctcctcc cagtcgaact gcacgccggt cgcctcgagg acccggcggg tggcctcgga
     1081 gagctcgggc ccgaccccgt cgccggggat gaacgtcacg cggtgcgcca tggcaggtct
     1141 ccttcgactc cgtgtcccgg catcatatcc gcggctccgg cagccctccg gacgggccac
     1201 gctgattggt agactcaccg gtaggaaccc gaaggaggct cgttgggcga gaagggactg
     1261 gccgacgtcg tcgccgccga gacgaagctc tcggacatcg acggcaagct gggcaagctg
     1321 ttctacgtcg gctacgacat ccacgacctg gcgcagcacg cgacgttcga ggagacgatg
     1381 tacctcctcc actacctgcg cctcccgaac cgcgacgagc tggacgacct caacgagcgc
     1441 ctcctcgcgg agcgggacct ggacgagttc accgcgggcc tcatgcccac gttcgccgag
     1501 gccgcctcgc ccatgtcgat gctgcggacg ggggtctccg ccgggtccgc ctacgacccc
     1561 gacggctggg acaactccgc cgaggcgaac gagcggaagg cgatccggct catggcgcgg
     1621 ttccccacga tgatcgccgc ctaccaccgc cttcggaccg ggcaggaccc gatcccgccg
     1681 aacccgaagc tcccccacgc ggcgaacttc ctcacgatgc tcctcggcga ggagcccgac
     1741 gaggaggtcg cgcgcgcctt cgacatctgc ctcgtcctgc acgccgacca cacgatgaac
     1801 gcctcgacct tcgccggccg cgtcgtcgcc tccacgctcg cggacatgca ctcggcgatc
     1861 accgcggcga tcgcggcgct gaaggggccg ctccacgggg gggcgaacga ggccgtgatg
     1921 aagatggtcg aggagatcgg cgacgtcggc cgcgccgagg cgtacgtgaa ggccaagctc
     1981 ggccgcaagg agaagatcat ggggttcggt caccgcgtct acaagaccga ggacccgcgc
     2041 gcgacgcacc tgcgccgcct ctcccgcgag ctcggccagc gcgccggcga cacccgctgg
     2101 tacgagatct cggagcggat cgagaaggtc gtgatggacg agaagggcct ctacccgaac
     2161 gtcgacttct acgcggcgag cgtctaccgc tcgatgggga tcccgaccga cctgttcacg
     2221 accgtgttcg ccgccagccg cgtctcgggc tggacggccc acgtgcgcga gcagctcgcc
     2281 gacaaccgcc tgatccgccc cgagtccgac tacatcggcc cgaccggcct ccgctacgtc
     2341 ccgatcgagg agcgcggatg aagatccgcg acatcatgac cgaggcggcg gtcaccgatc
     2401 agcccgacga cacgctcgcg tcggcggcgg cgaagatgtg ggagcagcag accggctcgc
     2461 tgctcgtgac gcagcaggac gagctcgtcg gcatcgtcac ggagcgggac gtgctcaagg
     2521 cggtcgcgac cgggacgccc ctcgacgcgc ccctcgggac cgtcatgtcg aaggatctcg
     2581 tcacggtcgg ccccggcacc tcgctgcgcg aggcggcgaa gatcatgacc gagaggtgga
     2641 tccgccacct gcccgtgctc gacggcggcc ggctcgtggg gatcgtctcc cagcgcgacc
     2701 tctccggcgt gctggcgggc gccctcaacg agcccgaggg gctgcaccgg ctgatcgaat
     2761 cgagcgagct ggtccgcgag cgacgtctgg cgcggatcga agccggggac ctggactagg
     2821 cagagaccgg cagccggatc ccgaagcagg ccccggtggg gtggttcggc tcgtaccagg
     2881 cgtccccgcc gtgcgccttc gccaggccct tcacgatcga gagaccgagc cccgtgccgc
     2941 cggacccacg cgtgttcgcg gtctcggcgc gcgagaacga ctcgaacagc tgcggaacga
     3001 actcgcgggg aacgccgggg ccctcgtccc ggacgaggat ctcgatggag tccccctcgt
     3061 cacggatctc cacctcgatg ggcgtctcgc cgtacttgta ggcgttcgtc acgaggttcg
     3121 tgagcatctg tcgcaggtgg tccgggtcgg cctcgagctt caggccctcg tccccgacga
     3181 cgtcgacgtc ctccgcgcac ggaagctccg ccacgatcga gccgacctcc tggagcgcgt
     3241 ccagggtctg cttctgcatc cgcacggacc caccctcgat ccgggagatc gtcaggaggt
     3301 cgccgacgag ccgggagagc cgctcggcct gctccaccac gaggccgagg tgctggcgcc
     3361 gctcgtcctc cgcgatgacc tcccagtgac gggagagggt cttcgcgaac cccaggaccg
     3421 aggtcagcgg ggaccggagc tcgtgcgcgg ccaccgcgac gaagcgggac ttctgctcgt
     3481 ccgcgcgccg gagctcatcg agcgtccgct cgagctcctc gcgcgccgtc cggcgggcct
     3541 ggaagtcgtg gatctgcacg acgaagcaca gcgggcgatc ctcgtcgtcg ctcacgagca
     3601 ggccggtgag gaacacggcc gtcgtgctgc cgtcggcagc ccggatccgg gcgtccatcc
     3661 cgaaggcgcg gccgcccccc gactccgcca gccgcatccg gctgagg
//
LOCUS       DATLGG010000034         6059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_129449, whole genome shotgun sequence.
ACCESSION   DATLGG010000034 DATLGG010000000
VERSION     DATLGG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129449"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VM638_00815"
     CDS             <1..260
                     /locus_tag="VM638_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11002.1"
                     /translation="HVPYVWRLEDLWVVNAGTCASLRLRGHTKPCYNVLEFEGGEVKI
                     HRKYPFGASTVIAHFSPSTGEQFYRELESLTQEPLSRAPVD"
     gene            359..1624
                     /locus_tag="VM638_00820"
     CDS             359..1624
                     /locus_tag="VM638_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HVM11003.1"
                     /translation="MGHEVAAALFVGGIEKVEREGEVDLGDIPVIRADGRHGEALGQA
                     ISDHGAEAVLDLSDEPVLGYRRRMEMAAVALAAGLPYLGADFTLEPAVEGPPLPRPTL
                     GVIGTGKRTGKTAISGEAARIADRVGLRPVVVAMGRGGPPAPQVARAGSVSLDDLLRM
                     AGEGRHAASDYLEDAVTSGVTTVGARRCAGGLAGRPYVSNVREAAARALEENPGLVIL
                     EGSGSAMPPVPWDAGILVCPATAPEEHVAGYLGPYRLLLSDLVVLTMGAGPDVGPQHL
                     ATLRSHVSRLRPNARIVLTDLRPVPLKDVGGRKVYLATTAPRHAGDAMVSHLEEGFGC
                     EVVGITHHLADRARLREDLAAAPRFDVLLTELKAAAIDVAAEHARSVGAEVVFVDNRP
                     VTLEGDGDLPVLLEETARLATARAAERTT"
     gene            1621..2580
                     /locus_tag="VM638_00825"
     CDS             1621..2580
                     /locus_tag="VM638_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of 2-phospho-D-glyceroyl
                     phosphate from ATP and 2-phospho-D-glycerate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HVM11004.1"
                     /translation="MRKRGTHITISDRQTGLPYSKGLTASQVMVTGLSPDRAYQVAET
                     IEDHLVARGVDSVTSEELRALTARVLEEVAGDRYAKSFVRWQHVADLEVPLVLMIGGT
                     TGVGKSTVATQVATRLGVVRVIPTDAIREVMRGLFSRDLMPTLYTSSFDADSALREPP
                     PRPADEVIVGFREQTTAVAVGVRSLVERAAIEGTSAIIEGAHVVPGFIDLERFTDKVL
                     AVPLVITVEDEDLHRGHFVARSMDSAARPMERYLRKFSNIRKVQKYIKSQALSHGWPV
                     VPSYNLDQTIASVIDLVVERAEDWSARHARREVVVELEGGTSR"
     gene            2577..3242
                     /gene="fsa"
                     /locus_tag="VM638_00830"
     CDS             2577..3242
                     /gene="fsa"
                     /locus_tag="VM638_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HVM11005.1"
                     /translation="MKLFLDTANIEEIREINRWGVLSGVTTNPTLVGKEALRPEELWK
                     EILAEVDGDVSLETTEPEAAAMYEEGTRLAAMGENAVVKVPMTPAGLEAGRRLVDDGI
                     KINVTLVFSPAQAILAAEVGAYIVSPFLGRIDDAAADGMHALRQICEIYERQGYETQI
                     LAASLRHPMHVVESALAGAHIATMPYEVFAKLVKHPLTDLGLERFTRDWDELNRRLNE
                     EGA"
     gene            3245..4870
                     /gene="rho"
                     /locus_tag="VM638_00835"
     CDS             3245..4870
                     /gene="rho"
                     /locus_tag="VM638_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HVM11006.1"
                     /translation="MDTPTTDRSALQRKLLPELHGIAQSLGLTGTQRMKKGDLIAAIV
                     EASNGHARAGGSSAPAPKAEETRSTETPASGNGDSSERAPVAVGGRGEAASDVQEAPR
                     EPREPRPPREGGRRDEGEGRGPRRNQDRGGRGRDRGRGRDRRGRDRDRGRDRGPERIR
                     EATPEELAEAPIYTGILDILPEGYGFLRTSGYLPGSEDVYVSLSQIRRFALRKGDQVT
                     GAVRHPKDNEKYPALLRIDSVNGLDTDAARERPHFEKLTPLFPQERFRLESGPTAVTE
                     RIIDLVAPIGKGQRGMVVSPPKAGKTTVLKQIANGIIKSSPETHVMVLLVDERPEEVT
                     DWQRTVDAAEVVASTFDKPADQHIQVTDLVLERAKRLVEGGKDVAILLDSITRMARAY
                     NLAAPASGKILSGGVDSTALYPPKRFFGAARNIEEGGSLTIIASALVETGSRMDEVIF
                     EEFKGTGNMELRLDRNLSEKRIFPAINIEASGTRKEELLMPSEELALTWRLRRVLHAL
                     EGGASLEMLIDKVRATKSNAQFLLEISKAPVRD"
     gene            4872..5096
                     /locus_tag="VM638_00840"
     CDS             4872..5096
                     /locus_tag="VM638_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11007.1"
                     /translation="MAGWGDDPTLKELIEAISDGWKPVRIAEDRETPGGPFDVVTVEK
                     DGEQREYKSDHLHFHRYVEGLMDDYGLSYS"
     gene            5116..5376
                     /gene="rpmE"
                     /locus_tag="VM638_00845"
     CDS             5116..5376
                     /gene="rpmE"
                     /locus_tag="VM638_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HVM11008.1"
                     /translation="MRQGIHPDYKHATVRCSCGNTFDTRATVEEIRVEICSNCHPFYT
                     GKQKLVDAGGRVERFQRRYANQPKPSAPRRQEQSEAGDTAGE"
     gene            complement(5380..>6059)
                     /locus_tag="VM638_00850"
     CDS             complement(5380..>6059)
                     /locus_tag="VM638_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HVM11009.1"
                     /translation="PEASASIRGEILGWANQVFSAAFSQRWIVPVVERFLSGGAVHLL
                     LMGLAAALWMMGRVVHTTVEVIRIAYDEERRKRRSWRERLAFVRAAAVALLGALVIGS
                     VAVAGPKLIDAANPTVGLSVLLHTLLWTGVAALAGVYLWWLYGVAPDDGHQRRRLPGA
                     LTGTVLGGAGSVVVWLQASWLFAGGGVLGPLALPFVVMGWAYVLGFAALLGAEANRVA
                     EDRGESR"
BASE COUNT          976 a         2114 c         2180 g          789 t
ORIGIN      
        1 agcacgtgcc gtacgtctgg cggctcgagg acctgtgggt ggtgaacgcg ggaacgtgcg
       61 cgtcccttcg cctgcgcggc cacacgaagc cgtgctacaa cgtcctcgag ttcgaggggg
      121 gcgaggtgaa gatccaccgc aagtacccct tcggcgcatc caccgtgatc gcgcacttct
      181 ccccctcgac cggggagcag ttctatcggg agctcgagtc gctcacgcag gagccgttga
      241 gccgggctcc cgtcgactga cgccgaccca ccggaggcac ccgctgcgag tcatcgcact
      301 cgtcgatgga gagcactatc cgccgacgac ccgctggggt ctcgaggccg cccgcgcgtt
      361 ggggcacgag gtcgcggcgg cgctgttcgt cggcgggatc gagaaggtcg agcgcgaggg
      421 tgaggtcgac ctcggcgaca tccccgtgat ccgagcggac ggccggcacg gcgaggcgct
      481 cgggcaggcg atcagcgacc acggcgccga ggcggtgctc gacctctcgg acgagccggt
      541 gctcggctac cgccgccgga tggagatggc ggccgtcgcg ctcgcggcgg gcctgccgta
      601 cctcggggcc gacttcacgc tggagccagc cgtcgagggt ccgccgctcc cgcggccgac
      661 gctcggcgtg atcggcaccg ggaagcggac cggcaagacc gcgatctccg gcgaagctgc
      721 gcgcatcgcc gatcgggtcg gtctgcggcc ggtcgtcgtc gcgatgggcc gcggcggacc
      781 gcccgcgcca caggtcgccc gcgccgggtc cgtctcgctc gacgacctcc ttcggatggc
      841 gggggaggga cggcacgcgg cgagcgacta cctcgaggac gcggtcacct cgggcgtcac
      901 gacggtcggt gccaggcgtt gcgccggggg cctggcgggc cggccgtacg tctcgaacgt
      961 gcgggaggcg gccgcacgcg cgctcgagga gaacccgggg ctcgtgatcc tcgaggggag
     1021 cggttcggcg atgccgccgg tgccctggga cgcgggcatc ctcgtctgcc cggcgacggc
     1081 gcccgaggag cacgtcgccg gctacctcgg gccgtaccgc ctgttgcttt ccgacctggt
     1141 tgtccttaca atgggagccg gcccagatgt cgggccgcag cacctcgcca ctctcagatc
     1201 ccacgtctct cggttgcgac cgaacgcccg gatcgtgctc accgatctca ggcccgtccc
     1261 actgaaggac gtcggaggga ggaaggtgta tctcgctaca accgcgccgc ggcacgccgg
     1321 ggacgccatg gtctcgcatc tcgaggaggg tttcggatgc gaggtcgtgg gcatcaccca
     1381 ccacctggcg gaccgggcgc gattgcggga ggacctggct gcggcgccgc ggttcgacgt
     1441 cctgctgacc gagctgaagg cggcggcgat cgacgtcgcc gccgagcacg caaggtcggt
     1501 cggggccgag gtggtcttcg tcgacaaccg cccggtgacg ctggaggggg acggcgacct
     1561 gccggtgctg ctggaggaga cggcccgact ggcaaccgcg agggccgcgg agcgaacgac
     1621 atgaggaagc gcggaaccca catcacgatc tctgaccgcc agacggggtt gccctactcg
     1681 aagggcctca ccgcctcgca ggtcatggtc acgggcctat ccccggaccg ggcgtaccag
     1741 gtggcggaga cgatcgagga tcatctcgtc gcccgcggcg tcgactcggt gacgtccgag
     1801 gagctgcggg ccctcaccgc gcgcgtcctc gaggaggtcg ccggcgaccg gtacgcgaag
     1861 agcttcgtgc gctggcagca cgtcgccgac ctcgaggtcc cgctcgtgct gatgatcggg
     1921 gggaccaccg gcgtcgggaa gtccacggtc gccacgcagg tcgcgacccg cctcggcgtg
     1981 gtccgcgtga tcccgaccga cgcgatccgc gaggtcatgc gcggactgtt ctcccgcgac
     2041 ctgatgccca ccctctacac ctcgtccttc gacgccgact ccgccctgcg ggagccaccg
     2101 ccgcgcccgg ccgacgaggt gatcgtgggc ttccgcgagc agaccaccgc cgtcgccgtc
     2161 ggggtccgct ccctcgtcga gcgcgcagcg atcgaaggga cgagcgcgat catcgaaggg
     2221 gcacacgtcg tgccgggatt catcgacctc gagcgcttca ccgacaaggt gctcgcggtg
     2281 ccgctcgtca tcaccgtcga ggacgaggac ctgcaccgcg gtcacttcgt cgcgcgctcg
     2341 atggactccg cggcgcgtcc gatggagcgc tacctccgga agttctcgaa catccgcaag
     2401 gtgcagaagt acatcaagag tcaggcgctc tcgcacgggt ggccggtcgt cccgagctac
     2461 aacctcgatc agacgatcgc ctccgtgatc gacctcgtcg tcgagcgcgc cgaggactgg
     2521 tcagcccgtc acgcgcggcg cgaggtcgtc gtggagctcg aaggaggaac gtcccggtga
     2581 agctgttcct ggataccgcg aacatcgaag agatccgtga gatcaaccgc tggggggtgc
     2641 tctccggcgt gacgacgaac cccacgctcg tcgggaagga ggcgctgcgc ccggaggagc
     2701 tgtggaagga gatcctggcg gaggtcgacg gcgacgtctc cctcgagacg accgagcccg
     2761 aggccgcggc catgtacgag gaggggacgc gcctcgcggc gatgggcgag aacgccgtgg
     2821 tgaaggtccc gatgaccccg gccggtctcg aggccgggcg gcggctcgtc gacgacggca
     2881 tcaagatcaa cgtgacgctc gtcttctcgc cggcgcaggc gatcctcgcg gccgaggtcg
     2941 gcgcctacat cgtgtcgccg ttcctcggcc ggatcgacga cgccgccgcc gacgggatgc
     3001 acgccctccg gcagatctgc gagatctacg agcggcaggg atacgagacg cagatcctgg
     3061 ccgcatccct gcgccacccg atgcacgtgg tggagtcggc cctcgccggc gcccacatcg
     3121 caacgatgcc gtacgaggtc ttcgccaagc tggtgaagca cccgctcacc gacctcggcc
     3181 tcgagcgctt cacgcgcgac tgggacgagc tcaaccggcg gttgaacgag gaaggagcct
     3241 gaacgtggac acccccacga cggatcggtc ggccctccag cggaagctcc tgccggagct
     3301 ccacgggatc gcgcagtccc tcgggctgac cggaacgcag cgcatgaaga agggcgacct
     3361 gatcgccgcg atcgtcgagg cctcgaacgg tcacgcgcgg gccggcggct cgtcggcgcc
     3421 cgctccgaag gccgaggaga cgcgatcgac cgagacaccc gcctccggca acggtgattc
     3481 gagcgagcgc gcgccggtcg cggtgggtgg ccgcggggag gcggcgtccg atgtccagga
     3541 ggctccccgc gagccccggg agccccgccc cccgcgggag ggtggccggc gggacgaggg
     3601 agagggccgc ggcccgcgtc gcaaccagga ccggggcggc cgtgggcgcg accgtggccg
     3661 tggccgggac cgccgcggac gcgaccggga ccgcggacgc gaccgcggcc ccgagcggat
     3721 ccgcgaggcg acgcccgagg agctggccga ggccccgatc tacaccggga tcctcgacat
     3781 cctgcccgag ggctacggct tcctccggac gagcgggtac ctccctggtt ccgaggacgt
     3841 ctacgtgtcg ctgtcgcaga tccgccgctt cgcgctccgc aagggcgacc aggtcaccgg
     3901 tgcggtccgt cacccgaagg acaacgagaa gtacccggcg ctgctgcgca tcgactcggt
     3961 caacgggctc gacaccgacg cggcgcgaga gcgcccgcac ttcgagaagc tgacgccgct
     4021 gttcccccag gagcggttcc gcctcgaaag cggcccgacc gcggtgacgg agcggatcat
     4081 cgacctcgtc gccccgatcg ggaaggggca gcgggggatg gtcgtgtcgc ccccgaaggc
     4141 ggggaagacg accgtgctca agcagatcgc gaacgggatc atcaagtcga gcccggagac
     4201 ccacgtcatg gtcctgctcg tcgacgagcg gccggaggag gtcaccgact ggcagcggac
     4261 ggtcgacgcg gcggaggtcg tcgcctcgac cttcgacaag ccggccgacc agcacatcca
     4321 ggtcaccgac ctcgtgctcg agcgggcgaa gcgcctcgtc gagggcggca aggacgtggc
     4381 gatcctcctc gactcgatca cccggatggc gcgggcctac aacctcgcgg cgccggcctc
     4441 ggggaagatc ctctccggtg gtgtcgactc gaccgcgctc tacccgccga agcggttctt
     4501 cggtgcagcg cggaacatcg aggagggcgg gtcgctcacg atcatcgcga gcgccctcgt
     4561 cgagacgggc tcccggatgg acgaggtgat cttcgaggag ttcaagggca ccggcaacat
     4621 ggagctccgc ctcgaccgga acctgtcgga gaagcgcatc ttcccggcga tcaacatcga
     4681 ggcctccggc acccgcaagg aggagctgct gatgccgagc gaggagctcg cgctcacgtg
     4741 gcgcctgcgc cgggtcctcc acgcgctcga gggcggcgcc tcgctcgaga tgctgatcga
     4801 caaggtccgc gcgaccaagt cgaacgcgca gttcctgctg gagatctcga aggccccggt
     4861 gagggactga catggcgggc tggggagacg atccgacgct gaaggagctg atcgaggcga
     4921 tctccgacgg ctggaagccg gtacggatcg ccgaggaccg cgagacgcct ggcgggccgt
     4981 tcgacgtcgt caccgtcgag aaggacggcg agcagcggga atacaagagc gaccatctgc
     5041 atttccaccg gtacgtcgag gggctgatgg acgactacgg cctctcgtac tcgtgacgcg
     5101 aaggagctga ggaagatgag gcaaggcatc caccccgact acaagcacgc gacggtccgc
     5161 tgctcgtgcg gcaacacgtt cgacacgcgc gcgaccgtcg aggagatccg cgtcgagatc
     5221 tgctcgaact gccacccctt ctacacgggg aagcagaagc tcgtggacgc cggcggccgc
     5281 gtcgagcggt tccagcggcg gtacgcgaac cagccgaagc cgtcggcgcc gcggcgtcag
     5341 gagcagtcgg aagctgggga caccgcgggc gagtaggggc tagcggctct cgccgcggtc
     5401 ctcggcgact cggttcgcct cggctccgag gagggccgcg aagcccagca cgtaggccca
     5461 ccccatgacc acgaacggca gcgccagggg cccgaggacc ccgccgccgg cgaacagcca
     5521 gctggcctgg agccagacga cgacgctgcc cgctccgccg aggaccgtgc ccgtcagcgc
     5581 acccggaagg cgccgccgct ggtggccgtc gtcgggcgcg accccgtaga gccaccacag
     5641 gtagacgccg gcgagagccg ccacccccgt ccagagcagg gtgtgcagca ggaccgacag
     5701 ccccacggtc gggttcgcgg cgtcgatcag cttcggcccg gccaccgcca cgcttccgat
     5761 caccaaggcg ccgaggagcg cgaccgcggc ggctcgtacg aaggcgagcc gctcccgcca
     5821 ggagcgacgc ttccggcgct cctcgtcgta cgcgatgcgg atcacctcga ccgtcgtgtg
     5881 cacgacccgg cccatcatcc agagcgcggc ggcgagcccc atcagcagga gatgcacggc
     5941 gccgccggag aggaagcgct cgacgaccgg gacgatccac cgctgggaga acgccgcgga
     6001 gaagacctgg ttcgcccagc cgaggatctc tcccctgatg ctcgccgatg cctcagggg
//
LOCUS       DATLGG010000035         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_81852, whole genome shotgun sequence.
ACCESSION   DATLGG010000035 DATLGG010000000
VERSION     DATLGG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81852"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..742)
                     /locus_tag="VM638_00855"
     CDS             complement(356..742)
                     /locus_tag="VM638_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11010.1"
                     /translation="MIRLAVLAAVAAILPACDAQEGPRGVRGPKILPRDPNAPYVITA
                     IDYHFHDAHPTPRLSPEREVIVSNQGRNLHNVTFEELGFSRDLEPGDRISLGKIGELL
                     ERPGDHPLVCVYHVDREMTGLIRVAG"
     gene            complement(739..1476)
                     /locus_tag="VM638_00860"
     CDS             complement(739..1476)
                     /locus_tag="VM638_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVM11011.1"
                     /translation="MSDAVVRLEGIHKWFGDLHVLRGIDLAVTTGEVLAVIGPSGSGK
                     STLLRTINLLEEPSQGKVWFGGRDITDIRTDLNKLRTQIGIVFQAFNLFPHKTARQNI
                     TLALEKVLRLDAEEARNRAMEQLEHVGLVEKADAYPGQLSGGQQQRVAIARALAMRPR
                     LMLFDEVTSALDPELVGEVLEVMKRLADEGMTMVVVTHEMGFARDVSSRLVFMDEGRI
                     IEEGDPRRIFDRPAEARTRKFLARVLP"
     gene            complement(1473..>2366)
                     /locus_tag="VM638_00865"
     CDS             complement(1473..>2366)
                     /locus_tag="VM638_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVM11012.1"
                     /translation="GILSPATRTLALISAGLGLVACVAGSAMYRRMPTKRSREHAVAG
                     AVLGVQALLLVGVLLWFRAGDPERFAFHFFNLEKLEGYGGAFLRAAKNTVILAFVGEA
                     GGIVLGLILAMLAISSRAVVRAPARVYINFFRGTPLIWQLSTIYFLFLFGFRIRFSTF
                     VVAMIVFALNTAAYAAEVFRAGIQSVERGQMEASRSLGMSYLQAMRYAIVPQAIRRVI
                     PPLMNEFVILIKDTSLVIALGLAVSELELYNLARQGYADTFNATFFVAAAAGYLAITL
                     PLIGIVNAVERRLRSGLVGVV"
BASE COUNT          397 a          802 c          820 g          347 t
ORIGIN      
        1 cggtgacgtc ccgcggttct gtcgaacttt ccccgacggc aggcggaatt tttcgatgcg
       61 accgtccctg gccgctacct gcgctgatgc ttcataaccg ttgcgccgca acgtatctga
      121 tcgggttgac accctccgag cgccctgata ctttcgcgtc ggtcccgggc ggcgcgaagg
      181 aacagacgca ccggatcgga tttctccgga gacacgaatc ggagccgaag agccggaacg
      241 ttgctcggga ccaacgtttg gcgggtccgg gaagccgagt agataacgct cgcgtatggg
      301 gaaggcgtga gcggaaccaa aggcctcccg gacccgtctt tcatgtccgg acccttcagc
      361 ccgcgacgcg gatcagcccc gtcatctccc ggtcgacgtg gtagacgcag accagcgggt
      421 ggtccccggg ccgctcgagc agctccccga tcttcccgag cgagatccgg tcccccggct
      481 ccagatcgcg gctgaacccg agctcctcga acgtcacgtt gtggaggttg cggccctggt
      541 tcgagacgat cacctcccgc tcgggcgaca gccggggcgt cggatgcgcg tcgtggaagt
      601 ggtagtcgat cgccgtgatc acgtagggcg cgttggggtc gcgcgggagg atcttcggcc
      661 cccggacgcc gcgggggccc tcctgagcgt cgcaggccgg caggatggcg gcgacggccg
      721 cgaggacggc caggcggatc acggcaggac ccgcgcgagg aacttccggg tccgggcctc
      781 cgccgggcgg tcgaagattc gccgagggtc gccctcctcg atgatccggc cttcgtccat
      841 gaacacgaga cggctgctga cgtcgcgggc gaagcccatc tcgtgcgtca cgacgaccat
      901 cgtcatccct tcgtccgcga ggcgcttcat gacctcgagc acctccccga ccagctccgg
      961 gtcgagggcg gacgtgacct cgtcgaagag catcagccga ggccgcatcg cgagggctcg
     1021 cgcgatcgcc acgcgctgct gctgcccgcc ggagagctgt cccgggtagg cgtccgcctt
     1081 ctcgacgagc cccacgtgct cgagctgctc catcgcccgg ttccgggcct cctcggcgtc
     1141 caggcgcagc accttctcga gcgcgagggt gatgttctgc cgcgcggtct tgtgcgggaa
     1201 gaggttgaag gcctggaaga cgatcccgat ctgcgtgcgg agcttgttga ggtccgtgcg
     1261 gatgtcggtg atgtcccggc cgccgaacca gaccttcccc tgcgaaggct cttccagcag
     1321 gttgatcgtc cgcaggagcg tcgacttccc cgaccctgac ggcccgatca cggcgaggac
     1381 ctcgcccgtg gtcacggcca gatcgatccc gcgcagcacg tggaggtcgc cgaaccactt
     1441 gtggatcccc tccaggcgga cgacggcgtc gctcacacga cccccacgag cccgctgcgg
     1501 aggcggcgct cgaccgcgtt cacgatcccg atcaggggca gcgtgatggc gaggtacccc
     1561 gccgccgccg cgacgaagaa ggtcgcgttg aaggtgtccg cgtagccctg gcgggccagg
     1621 ttgtagagct ccagctcgga gaccgccagc cccagcgcga tcacgagcga ggtgtccttg
     1681 atcaggatca cgaactcgtt catcaggggc gggatgacgc ggcggatcgc ctgcggaacg
     1741 atcgcgtagc gcatcgcctg gaggtaggac atcccgaggg agcgcgaggc ctccatctgc
     1801 ccccgctcga cggactggat cccggcgcgg aagacctcag ctgcgtaggc ggcggtgttc
     1861 agggcgaaca cgatcatcgc caccacgaac gtcgagaacc ggatccggaa gccgaacagg
     1921 aacaggaagt agatcgtcga gagctgccag atgagcggcg tcccgcggaa gaagttgatg
     1981 tagacgcgag cgggggcgcg cacgacggcg cgcgaggaga tggcgagcat ggcaaggatc
     2041 aggccgagga cgatgccccc ggcctccccc acgaacgcga ggatgaccgt gttcttggcg
     2101 gcacgcagga acgcgccccc gtacccctcg agcttctcga ggttgaagaa gtggaaggcg
     2161 aagcgctccg gatccccggc acggaaccac agcaggacgc cgaccagcag gagcgcctgg
     2221 accccgagga cggcgccggc cacggcgtgc tcgcggctgc gcttcgtcgg catccgccgg
     2281 tacatcgcgg accccgccac gcacgcgacg agcccgaggc ccgccgagat gagggcgagg
     2341 gtgcgcgtcg cgggcgacag gatccc
//
LOCUS       DATLGG010000036         4444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_144039, whole genome shotgun sequence.
ACCESSION   DATLGG010000036 DATLGG010000000
VERSION     DATLGG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144039"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VM638_00870"
     CDS             complement(<1..384)
                     /locus_tag="VM638_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM11013.1"
                     /translation="MEISLIATVRDASPHVHDFLDSVRSQTRAPDEVVIVDGGSTDGT
                     LGILRGAEGVRVIEAPGANIARGRNLAIEAASHDHIAVSDADCVLDPRWLEHLEETLA
                     DGADVAMGFYRPLATTVFQAAAAAWN"
     gene            complement(564..1349)
                     /locus_tag="VM638_00875"
     CDS             complement(564..1349)
                     /locus_tag="VM638_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11014.1"
                     /translation="MKTARRFAPFVFFLTTACLGSRGASPVAAPSPFPSPSIPPPRTH
                     YVAPTFEEAGRTVMPLTFPDGSQAELVYEPKLGLSNASPQFFTSFIDGCPSDITGAAR
                     GMPSGDPVDLLRHPQGGFTHVYENDDGYYDVVLDIEDEPWTVRLWTEEKDSAEGCANS
                     LRVERAGGWVIVRAVGAARVASVGDHGGPQIGLINIGGNHERWILLFPERCTPSEVDG
                     DQTFADWCDASGLMRVHVYSDSREFIDRVREGLEIRNICHAAG"
     gene            complement(1368..2486)
                     /locus_tag="VM638_00880"
     CDS             complement(1368..2486)
                     /locus_tag="VM638_00880"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HVM11015.1"
                     /translation="MASVGMHVDQVFYRAPGGIGTYIRELVPAMSAADPTLDLTLFHA
                     AFPYATRPGWLDGYTVVTLPQQIRRLYPMWDALGRPPLPPQLGRLNVIHAPSPATVPP
                     VADGQRLVVTVHDLAFRVYPSLFPAAWRMLFRAGTWRAARKADALITVSENTASDLIR
                     FYGVEPDRVHVIPLAASLPETTGAGTETLERLRVRPPYVLFVGTLEPRKNLVRLVRAY
                     RRAVAEGGFPHQLVLAGPLGWHPQRLLAELKGGGPGEVVLAGRTRTEDLDTLYRSAEI
                     FAYPSLYEGFGLPVLEAMVRGVPVLTSTSSSLPEVAGDAALSVNPRSVTAIAEALAEL
                     MSNGPERERLSKAGLARAESSSWTRTANETLKVYKGLT"
     gene            2582..4267
                     /locus_tag="VM638_00885"
     CDS             2582..4267
                     /locus_tag="VM638_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM11016.1"
                     /translation="MSERPSRRPLASIALAILVATVAPASVVSAEDSPKDPMRMAPTC
                     IDGRATVRTTEGLPLVVGPCGESGSDVQGPAPEVPEVPASGPLDCVVPGYDDRCEAWT
                     APRYDGPWPGSNDYPGFGSFDRVRTVIAHPSKDLVFVGGTSTYTNGGLPDADFVEIAY
                     RASTGQPVWATTLEGIEDRTIAYQTSFALSPDGERLYAVGTAAVPGVYSYASVIAAFD
                     TDTGRFLWGRRTPSSAQSVETASVTLPDETVEERIFVGGVGRAISALDPSDGSTIWTS
                     VFPRGPHGARFNEIAVSPDGSRVFATGGEHRPDGFAGNFTTVAFDAIEGEQLWVARDE
                     LTQPDGFFGNNGASDLKVTPDGSRVIIAGLDPRTGTMLGSDASPILTVAYEAGTGERA
                     WRRSYGGPVEGETHFYFTLFQGMMDLSPDGRTVVLTAAINSHNSTGTVAYDVATGAQK
                     WGVESVEYAYVVVNHIGYYPSVVVSGDRAYVSNRRGIGYSQFRAVTTAYDLGDGNLDW
                     TARLGTNRTLFGGSALTGDRKRLVVASADHALSPGFPAPNVDTLDILTAAYDA"
BASE COUNT          662 a         1550 c         1575 g          657 t
ORIGIN      
        1 gttccaggcc gcggccgcgg cctggaacac cgtggtggcg agcggacggt agaagcccat
       61 cgccacgtcc gccccgtccg cgagcgtttc ctccaggtgc tccagccacc gagggtccag
      121 cacgcagtcc gcgtccgaga ccgcgatgtg gtcgtgcgaa gcggcctcga tcgccaggtt
      181 ccgcccccgc gcgatgttcg cccccggagc ctcgatcacc cgcacgccct cggcgccccg
      241 caggatgccc agcgtgccat ccgtcgaccc gccgtccacg atcaccacct cgtccggagc
      301 gcgcgtctgc gaccgcaccg agtccaggaa gtcatgcacg tgcggcgacg catccctcac
      361 cgtcgcgatc aacgagatct ccacccgggc agtgtaccgg tcgagccgcg gtgctctgcc
      421 ggcccgtagg tcgctcatcc cggcccgcag ctcggccagc tccctctggt tgcgacctca
      481 gtccagggga ccgccgtcat cagggtcatg agcgtctccg cgttcgcggc tcataaggcc
      541 tccagaggcg cccgacacga cccttagccg gcggcgtggc agatgttgcg gatctcgaga
      601 ccttcgcgga cccggtcgat gaattcccgg ctgtcggagt acacgtggac gcgcatcagg
      661 ccgctcgcgt cgcaccagtc ggcgaacgtc tgatcgccat cgacttccga gggcgtgcat
      721 cgttcgggga acagcaggat ccatcgctcg tggttgccgc cgatgttgat cagcccgatc
      781 tgcggcccgc cgtggtcgcc gaccgatgcg acgcgtgccg cccccaccgc gcgaacgatc
      841 acccatcccc ccgctctctc gacccgcagg ctgttcgcgc atccctcggc tgagtccttc
      901 tcctccgtcc acagacgcac cgtccatggc tcgtcttcga tgtcgaggac gacgtcgtag
      961 tagccgtcgt cgttctcgta gacgtgggtg aaccctccct gcgggtgccg aagcaggtcg
     1021 accggatccc cggacggcat ccccctcgct gcgccggtga tgtcgctcgg gcagccgtcg
     1081 atgaagctcg tgaagaactg gggcgacgcg ttggatagcc ccagcttcgg ctcgtagacg
     1141 agctcggcct gactgccgtc cgggaaggtg aggggcatga ccgttcgtcc cgcttcctcg
     1201 aacgtcggcg cgacgtagtg ggttcgcggc ggggggatcg atggtgatgg aaacggcgac
     1261 ggcgccgcga cgggggaggc gccgcgagag ccgaggcagg ccgtcgtcag gaagaagacg
     1321 aagggcgcga acctgcgggc tgtcttcatg gtccctccgt gacagggtta cgtcaggccc
     1381 ttgtacacct tcagggtttc gttcgcggtg cgggtccagg aggagctctc ggctctggcg
     1441 aggccggcct tggagaggcg ttcccgttcg gggccgttcg acatgagctc ggcgagggcc
     1501 tcggcgatcg cggtgacgga gcgggggttg acgctgagag cggcgtcgcc ggctacctcg
     1561 ggcagggagg aggaggtcga ggtgaggacg gggacgccgc ggaccatcgc ctccaggacc
     1621 ggtaggccga agccctcgta gagcgagggg taggcgaaga tctcggcgga gcggtagagg
     1681 gtgtcgaggt cctcggtgcg tgtgcggcct gcgagcacga cctcgccggg gccgccgccc
     1741 ttgagctccg ccagcaggcg ctgggggtgc catccgagtg ggcccgcgag aacgagctga
     1801 tgcgggaacc ctccctcggc caccgcacgc cggtacgcgc ggaccaggcg tacgaggttc
     1861 ttccgcggct cgagcgtccc gacgaacagc acgtagggcg ggcgcacgcg caggcgctcc
     1921 agcgtctcgg tgccggcgcc cgtggtctcg gggagcgagg cggcgagcgg gatgacgtgc
     1981 acgcggtccg gctcgacgcc gtagaagcgg atcaggtcgg aggcggtgtt ctccgacacg
     2041 gtgatcagcg cgtcggcctt gcgcgcggct cgccacgtcc ccgcacggaa gagcatccgc
     2101 caggccgccg ggaagaggct cgggtacacg cggaacgcga ggtcgtggac ggtcacgacg
     2161 agccgctgtc cgtcggcgac cggcggcacg gtggcgggcg acggcgcatg gatgacgttc
     2221 aggcgaccca gctgcggcgg caggggcggc cgtccgaggg cgtcccacat cgggtacagc
     2281 cgccggatct gctgggggag ggtcacgacc gtgtagccgt cgagccaccc gggccgggtc
     2341 gcgtacggga aggccgcgtg gaacagcgtg aggtcgagcg tcggatccgc cgccgacatc
     2401 gccgggacga gctcgcggat gtaggtcccg atcccccccg gcgcccggta gaacacctgg
     2461 tccacgtgca tgccgacgga cgccatcgcc gctgacccta ccggaccttt gtgcagccgc
     2521 ccaaaggtgt caggacacgc ggcggcgaat ccatgctcga cctgacgacc aggaggagca
     2581 gatgtccgag cgcccgtccc gccgccccct cgcctcgatc gcgctggcga tcctggtcgc
     2641 caccgtggcc cccgcgtccg tggtctccgc ggaggactcg ccgaaggacc cgatgcggat
     2701 ggccccgacg tgcatcgacg gccgcgcgac cgtccgcacg acggagggac tcccgctcgt
     2761 ggtcggccca tgcggcgaga gcggtagcga cgtgcagggc ccggcccccg aggttccgga
     2821 ggttccggcg tcggggccgc tcgactgcgt cgtcccaggc tacgacgacc gctgcgaggc
     2881 gtggaccgct ccccgctacg acgggccgtg gccgggaagc aacgactatc cgggcttcgg
     2941 cagcttcgac cgggtccgga ccgtcatcgc gcatccctcc aaggacctcg tgttcgtcgg
     3001 ggggaccagc acgtacacga acggtgggct ccccgacgcc gacttcgtcg agatcgcgta
     3061 ccgcgcttcc acgggtcagc ccgtctgggc gacgaccctg gaggggatcg aggaccggac
     3121 catcgcctac cagacctcct tcgcgctgtc ccccgacggc gagcggctct acgcggtggg
     3181 caccgcagcc gtccccggtg tgtactccta tgcttccgtg atcgctgcct tcgacacgga
     3241 cacggggcgg ttcctctggg gaaggcggac cccgagctcc gctcagagcg tcgagaccgc
     3301 gtccgtcacg cttcccgacg agaccgtgga ggagcgcatc ttcgtcggcg gggtgggacg
     3361 cgcgatctcc gcgctcgacc cctcggacgg atcgacgatc tggacctccg tcttcccgag
     3421 agggccccat ggggcgaggt tcaacgagat cgccgtcagc cccgacgggt ctcgcgtgtt
     3481 cgccacgggc ggtgagcacc ggcccgacgg cttcgcgggg aacttcacga ccgtggcgtt
     3541 cgacgcgatc gagggcgagc agctctgggt cgcccgcgac gagctcaccc agccggatgg
     3601 gttcttcggc aacaacggtg cgagcgatct caaggtgacg ccggacggct cgcgcgtcat
     3661 catcgccggg ctcgaccccc ggacggggac gatgctcggg agcgacgcga gcccgatcct
     3721 gacggtggcc tacgaagcgg ggacgggcga gcgtgcctgg cgccgctcgt acggtggccc
     3781 ggtcgagggc gagacgcact tctacttcac gctcttccag gggatgatgg atctgagccc
     3841 cgacgggcgg acggtcgtgc tgaccgccgc gatcaacagc cacaacagca cggggacagt
     3901 cgcctacgac gtggcgaccg gcgcgcagaa gtggggggtc gagtcggtcg agtacgccta
     3961 cgtggtcgtc aaccacatcg gctactaccc ctcggtggtg gtgagcggag accgcgcgta
     4021 cgtatccaac cgacgcggca tcgggtactc ccagttccga gcggtcacga ccgcgtacga
     4081 cctcggtgac gggaacctgg actggacggc ccggctcggg acgaaccgaa cgctgttcgg
     4141 cggcagcgct ctcaccggcg accgcaagcg gctggtcgtg gcgagcgccg accacgcgct
     4201 gtcgcccggg ttccccgccc cgaacgtcga cacgctcgac atcctgaccg ccgcctacga
     4261 cgcctgatca gtcgtcctcg aagtcctcgg ggtcggactc ctcgatctcg cggaggatct
     4321 tcctcgcctc ctcgagccgg gtctcgggga cgaggacgcg gatcggctgg cggagcacga
     4381 tcgaggtcgc ggctccatag tactcgccca cctccggagg gtcgatgaac gcggtgatcc
     4441 cctc
//
LOCUS       DATLGG010000037         5288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_191372, whole genome shotgun sequence.
ACCESSION   DATLGG010000037 DATLGG010000000
VERSION     DATLGG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191372"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="deoC"
                     /locus_tag="VM638_00890"
     CDS             complement(<1..735)
                     /gene="deoC"
                     /locus_tag="VM638_00890"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HVM11017.1"
                     /translation="MSLDSRSILELAREMDLDDRRVAERARRLMDVPAPPAEDPAPLI
                     EVLRCLDLTTLEPDDTPDRVRALCARARSLDPSPAAVCVLPHLVEVAARELGGSGIRV
                     AGVAGDFPAGEAPLDRKVAEVRRVREAGAGEVDVVIDRRALLERGPSAALDEVRSLVE
                     AAEGALVKVILETGALDARASAEGALAALAAGAAFVKTSTGKRQPGATSERALVLMEA
                     VRAVEAAGGPSGGVKVAGGVRTPEQAL"
     gene            812..1234
                     /locus_tag="VM638_00895"
     CDS             812..1234
                     /locus_tag="VM638_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVM11018.1"
                     /translation="MKRVLLIVALATAGCDALPPPPEAGPSPGTPLDQPMRGTTPTPT
                     ACHDISAAQAGPVAVIMQDNSYSPDCFAISSTQAIQLANDGETRHSFTIADAVDVVVE
                     PGNRTETEPMEDLTGPGTFDFICKYHEDDGMVGVVIVR"
     gene            1252..1647
                     /locus_tag="VM638_00900"
     CDS             1252..1647
                     /locus_tag="VM638_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVM11019.1"
                     /translation="MRTKALVLASIFLLAACGGGGGDPTVGGQPAEGTGGGRACTDLS
                     TGAEARLVMVDNAFEPPCLIVSTTQTIALPNEGGALHNFQVEEQDIDIDVPPGEQAQT
                     EPIGDVMEPGTYRFVCKYHPGMDGELQVR"
     gene            complement(1649..2530)
                     /gene="era"
                     /locus_tag="VM638_00905"
     CDS             complement(1649..2530)
                     /gene="era"
                     /locus_tag="VM638_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVM11020.1"
                     /translation="MTFRSGIVPVLGRPNTGKSSLVNALVGRKVSIVSDKPQTTRGRI
                     RGVSSGDGWQIVFTDTPGYHKPRTLLGERLNDVVGESTTDADAVVHVVDAAAGVGRGD
                     AHVATTRVATLACPKVCAVNKIDRLKRGEVIPQLEAAARLTDYDHIVPVSARTGAGLP
                     DLRRLLAELLPEGPRYFPPGEVTDQSVEHQVGEIVREKALHLTREELPHSIAVDVEEM
                     ARDEDLVRISALVLVERPSQKGIVIGRGGEMLKEIGTKARLELEALLGSRVFLELRVK
                     VQKDWQRDPAALNRLGL"
     gene            complement(2527..2931)
                     /locus_tag="VM638_00910"
     CDS             complement(2527..2931)
                     /locus_tag="VM638_00910"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HVM11021.1"
                     /translation="MSAADPAEELLEAARLARRAAYAPYSGFPVGAAVLDESGQVHAG
                     ANVENASYSLAACAERNAVQRAVFTGARALVAVAVAGPGREVTWPCGACLQVLHEFGP
                     EMRVIVEAADGRREERPLPELLPRPFGPADLR"
     gene            complement(2918..4165)
                     /locus_tag="VM638_00915"
     CDS             complement(2918..4165)
                     /locus_tag="VM638_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVM11022.1"
                     /translation="MGVLVVLGSLLAVAETSISRMSRIRAMALEEDGRRNASLLVEIE
                     GDPPRFLNAIYLAVMLAQNGSAILVAIIADRLYGSLGITLLSAGFTLLYFVIVEAMSK
                     TFGILHSDRAALALSPVVWFLGRALAWPTRLLIGLANILLPGKGLKQGPFVSEEEIRS
                     MAEVGSEEGAIEKDEKEYIHAIFEFGDTVVREVMVPRPDMVSMDVDSPLDDALDVVIR
                     HGYSRIPVYRGSADEILGVVYAKDVLRHLRNRRTDRSLADIARKPYFVPESKKVSDLL
                     KEMQREKFHLAVVVDEYGSVVGLVTLEDLIEEIVGEIADEYDREEPNVEPAGDDRFRV
                     NARLPVDELNDLLDVNLPNTEWDTVGGLMMGILGRLPAQGEEVEFENLRFKAERVQGR
                     RIAKIVIERTNGDGAVEEAVERG"
     gene            complement(4183..4608)
                     /gene="ybeY"
                     /locus_tag="VM638_00920"
     CDS             complement(4183..4608)
                     /gene="ybeY"
                     /locus_tag="VM638_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVM11023.1"
                     /translation="MSERVTDDPPSPRVAVSDRQDLPVEAEALAELAAATMRGEGAAG
                     ELALSFVSVEEMASLHERYLGEPGPTDVLAFPQGEDDLLGDVVVCPAYAQGQNPDLGA
                     ELRLLVVHGVLHLLGYDHEAEEERAEMWARQERYAGVRA"
     gene            complement(4601..>5288)
                     /locus_tag="VM638_00925"
     CDS             complement(4601..>5288)
                     /locus_tag="VM638_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVM11024.1"
                     /translation="SLNVLSSRGRTIRPKTLNQKRYVDAIDAHTIVFGLGPAGTGKTY
                     LAVAKAVQALQTKQVDRIILTRPAVEAGERLGFLPGTLYEKIDPYLRPLYDALRDMMD
                     ADAVPRLLTDGVIEVAPLAFMRGRSLNSAFIILDEAQNTSPEQMKMFLTRLGFGSKAV
                     VNGDVTQIDLPAGQRSGLVVVEEILQGIDGIEFIHLGARDVVRHKIVMDIVEAYRRSS
                     ERERRAQGGE"
BASE COUNT          787 a         1909 c         1836 g          756 t
ORIGIN      
        1 gagcgcctgc tcgggggtgc ggacgccgcc ggcgaccttc acgccgccgg acggcccacc
       61 cgcagcctcc acggcccgca ccgcctccat caggacgagg gcgcgctcgg acgtcgcacc
      121 gggctgccgc ttgccggtcg aggtcttgac gaaggccgcc ccggcggcga gcgcggccag
      181 ggccccctcc gcggaggcgc gcgcgtccag cgcccccgtc tcgaggatca ccttcacgag
      241 cgccccctcg gcggcttcca cgagcgaccg gacctcgtcg agcgctgccg agggacctcg
      301 ctcgaggagc gcgcgccggt cgatcacgac atcgacctcg ccggccccgg cctcgcggac
      361 gcggcggacc tcggcgacct tgcgatcgag gggagcctcg ccggcgggga agtcgcccgc
      421 gacccccgcg acacggatgc cgctcccacc cagctcgcgc gccgccacct cgacgaggtg
      481 cggcaggacg cacacggcgg cgggcgaggg atcgagcgat ctcgcgcgcg cacagagcgc
      541 ccgcacccgg tcgggcgtgt cgtcgggctc cagcgtcgtc aggtcgaggc atcgcaggac
      601 ctcgatcagc ggggccgggt cctccgccgg gggcgccggg acgtccatca gccggcgtgc
      661 gcgttcggcg acacgccggt cgtccagatc catctcgcgc gccaactcga ggatcgagcg
      721 gctgtccagg ctcatcacct cccgggaggc gccgatgata gccggtgcgg ttgcaggttt
      781 aggagagcga gcaaccgggc aggaggaccg catgaagcgc gtgctgctca tcgtcgcgct
      841 ggccacggcc ggctgcgacg cgctcccgcc gccaccggaa gcgggcccga gcccgggaac
      901 cccgctcgat cagccgatgc gcgggaccac cccgacgccg accgcctgcc acgacatctc
      961 cgcggcgcag gcgggacccg tggccgtgat catgcaggac aacagctact cccccgactg
     1021 cttcgcgatc tcctcgaccc aggcgatcca gctcgcgaac gacggcgaga cccgccacag
     1081 cttcacgatc gccgatgcgg tggacgtcgt ggtcgagccg gggaaccgga cggagacgga
     1141 gccgatggag gacctgaccg gtccggggac cttcgacttc atctgcaagt accatgagga
     1201 cgacggcatg gtcggcgtcg tcatcgtccg gtagagcgaa aggggtcccc cgtgcgcacg
     1261 aaggctctgg tgctggcgtc catcttcctg ctcgccgcct gcggcggggg cggcggcgac
     1321 ccgaccgtcg gcgggcagcc cgccgagggg acgggcggtg gccgggcctg caccgacctc
     1381 tccaccggag ccgaggcgag gctcgtcatg gtcgacaacg cgttcgagcc gccctgcctg
     1441 atcgtgtcca ccacgcagac gatcgcgctc cccaacgaag gcggggccct gcacaacttc
     1501 caggtggagg agcaggacat cgacatcgac gtgcctcccg gcgagcaggc acagaccgaa
     1561 ccgatcggcg acgtgatgga accgggcacc taccggttcg tctgcaagta ccaccccggg
     1621 atggacgggg agctgcaggt ccgctagctc acaggccgag gcggttgagc gccgccgggt
     1681 cgcgctgcca gtccttctgc accttcacgc ggagctcgag gaagacgcgg cttccgagca
     1741 gcgcctcgag ctcgagccgg gccttcgtcc cgatctcctt cagcatctcc ccaccgcgcc
     1801 cgatcacgat ccctttctgc gagggccgct cgacgaggac cagcgcggag attcggacga
     1861 ggtcctcgtc gcgcgccatc tcctcgacgt cgaccgcgat cgagtgcggg agctcctcgc
     1921 gcgtcaggtg cagcgccttc tcgcgcacga tctcgccgac ctggtgctcg accgactggt
     1981 ccgtcacctc gcccggcggg aagtagcggg gaccctccgg taggagctcg gccaggagac
     2041 ggcgcagatc gggaaggccg gcgccggtcc gggccgagac cggcacgatg tggtcgtagt
     2101 ccgtgaggcg cgcagcggct tcgagctgcg ggatgacctc gccgcgcttg agacggtcga
     2161 tcttgttgac ggcgcagacc ttggggcagg caagggtcgc gacccgggtc gtggcgacgt
     2221 gggcgtcgcc ccgcccgaca cccgctgcgg catccacgac gtgcacgacc gcgtcggcat
     2281 cggtcgtgct ctcgccgacg acgtcgttca gccgctcccc gagcagcgtc cgaggcttgt
     2341 ggtacccggg cgtgtcggtg aagacgatct gccatccgtc tcccgacgag acgccgcgga
     2401 tccggcctcg cgtggtctgg ggcttgtccg acacgatcga gaccttgcgg ccgacgaggg
     2461 cgttcaccag gctcgacttg ccggtgttcg gccgcccgag gaccgggacg atgccggagc
     2521 ggaaggtcac cgcaggtcgg ccggaccgaa cggccgcggc aggagctcgg gaagggggcg
     2581 ctcctcacgc cggccgtcgg cggcctcgac gatcacccgc atctccgggc cgaactcatg
     2641 gaggacctgc aggcacgctc cgcaaggcca ggtcacctct cggccaggcc cggcaaccgc
     2701 cacggcgacg agcgcgcgcg ctccggtgaa gacggcgcgc tggacggcgt tccgctccgc
     2761 gcacgccgcc agcgagtacg aggcgttctc gacgttcgcg cccgcgtgca cctgcccgct
     2821 ctcgtcgagg acggcggctc cgaccgggaa gccgctgtag ggcgcgtacg cggcccggcg
     2881 ggcgagcctc gccgcttcca gcagctcctc ggcgggatca gccgcgctca acggcctcct
     2941 ccaccgcgcc atcgccgttc gtccgctcga tcacgatctt cgcgatccgc cgcccctgca
     3001 cgcgctccgc tttgaaccgg aggttctcga actcgacctc ctctccctgg gccgggagcc
     3061 ggccgaggat gcccatcatg aggccgccga cggtgtccca ctccgtgttc ggcaggttca
     3121 cgtcgagcag gtcgttcagc tcgtcgaccg gcagccgggc gttcacccgg aagcgatcgt
     3181 cgcccgcggg ctcgacgttc ggctcctcgc ggtcgtactc gtcggcgatc tcaccgacga
     3241 tctcctcgat caggtcctcg agcgtgacga gcccgacgac cgagccgtac tcgtcgacga
     3301 cgaccgcgag gtggaacttc tcgcgctgca tctccttcag gagatccgag accttcttcg
     3361 actcgggcac gaagtacggc ttgcgcgcga tgtcggcgag cgagcgatcg gtccgccggt
     3421 tccggaggtg gcgcaggacg tccttcgcgt agacgacgcc gaggatctcg tcggcgctcc
     3481 cccggtagac cgggatgcgc gagtagccgt ggcggatcac gacgtcgagc gcgtcgtcga
     3541 ggggggagtc gacgtccatc gagaccatgt ccggacgggg gaccatgacc tcgcggacga
     3601 cggtgtcccc gaactcgaag atcgcgtgga tgtattcctt ctcgtccttc tcgatggctc
     3661 cttcctcgga gccgacctcc gccatcgagc ggatctcctc ctcggagacg aacgggccct
     3721 gcttcaaccc cttgcccggg agcaggatgt tcgcgagccc gatcagcagg cgcgtcggcc
     3781 acgcaagggc gcgcccgagg aaccacacga ccggtgagag cgcgagcgcc gcccggtcgc
     3841 tgtgcaggat cccgaaggtc ttggacatcg cctcgacgat cacgaagtac agcaacgtga
     3901 agcccgcgga cagcagggtg atgccgaggc tgccgtagag acggtcggcg atgatcgcga
     3961 cgaggatggc cgagccgttc tgggcgagca tgaccgccag gtagatcgcg ttcaggaacc
     4021 gcggcgggtc cccctcgatc tcgacgagga gcgaggcgtt gcggcggcca tcctcctcga
     4081 gcgccatcgc ccggatgcgg ctcatgcggc tgatcgacgt ctcggcgacc gcgaggagcg
     4141 acccgagcac gacgagcacg cccacgacca ccatcatcca gatcacgcgc gaacccccgc
     4201 gtaccgctcc tgccgcgccc acatctccgc ccgctcttcc tcggcctcgt ggtcgtagcc
     4261 gagcaggtgg agcacgccgt ggacgacgag cagccggagc tcggccccca ggtcggggtt
     4321 ctgcccctgg gcgtacgcgg ggcagacgac gacgtcgccg agcaggtcgt cttcgccttg
     4381 cgggaaggcc agcacgtcgg tgggcccggg ctcgccgagg taccgctcat ggaggctcgc
     4441 catctcctcc acgctcacga acgagagcgc gagctcacca gcggctcctt caccgcgcat
     4501 ggtggcggcc gccagctcgg cgagggcctc cgcctcgacc ggcaggtcct gtcggtccga
     4561 gacggcgacc cggggactcg gagggtcgtc ggtcacgcgt tcactcaccg ccttgtgcgc
     4621 ggcgctcccg ctcggacgaa cgccggtacg cctcgacgat gtccatgacg atcttgtggc
     4681 gcacgacgtc gcgcgctccc aggtggatga actcgatgcc gtcgatgccc tggaggatct
     4741 cctcgacgac gacgagcccc gaccgctgcc ctgccggcag gtcgatctgg gtcacgtccc
     4801 cgttcacgac ggccttcgag ccgaagccga gccgcgtgag gaacatcttc atctgctccg
     4861 gcgaggtgtt ctgcgcctcg tcgagaatga tgaacgccga gttcaggctg cgaccccgca
     4921 tgaacgccag gggcgcgacc tcgatgacgc cgtcggtcaa caggcggggg accgcgtccg
     4981 cgtccatcat gtcgcgcagt gcgtcgtaca gcggccgcag gtacgggtcg atcttctcgt
     5041 acagggtgcc ggggaggaat ccgaggcgct cgcccgcctc tacggccggg cgggtcagga
     5101 tgatgcggtc gacctgcttg gtctgcagcg cctgcacggc cttcgccacg gcgaggtagg
     5161 tcttgccggt gccggcaggc cccaggccga agacgatggt gtgggcgtcg atggcgtcga
     5221 cgtagcgctt ctggttcagc gtcttggggc ggatggtccg gccgcgcgac gacagcacgt
     5281 tgaggctc
//
LOCUS       DATLGG010000038        12003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_19245, whole genome shotgun sequence.
ACCESSION   DATLGG010000038 DATLGG010000000
VERSION     DATLGG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19245"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VM638_00930"
     CDS             <1..260
                     /locus_tag="VM638_00930"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVM11025.1"
                     /translation="TSLRLARDLLGRVLVRDLGGTRLAGRIVEVEAYRPDDPASRVPP
                     RSRTSTRPSRSRARRSDVRSKNARGSASRSSLSVVLSRGTA"
     gene            complement(229..1953)
                     /gene="polX"
                     /locus_tag="VM638_00935"
     CDS             complement(229..1953)
                     /gene="polX"
                     /locus_tag="VM638_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HVM11026.1"
                     /translation="MPRANDEVEALLQEYSDLISISGGDAFKARAYEKAARAIGGHGG
                     DVAKLDLKGILAIPNVGRSIAEKVVEILRTGTFRALEEARADIPDGVRSLLAIPGLGP
                     KKALVLFDELGISSVEELLDALHAGRLRDLKGFGARTEENILRGVELMQQAGDRVPLG
                     VALDLAEELLAELRQNRAVGRAGYAGSLRRMRETIGDIDLLVASEGAEEVMDAFASLD
                     LVQEVIARGPTKCSVRTTKGLQVDLRVVEPAVWGAAMQYFTGSKAHNVRIREMAVRKG
                     LKLSEYGLFRVKSGRRLVAETEEEIYERLGLPWIPPTLREDRGEVEAAVAGEMPDLLQ
                     ERHLKGDLHTHTDLTDGLASLEKMLDAAAERGYAYYAVTDHAELLFMQRMTREKALAQ
                     RRRLAELQPRYPAMTLLHGSELNIQPDGSVDWDEEFLSGFDVLVASVHSHFNQSKEEM
                     TRRIIRAMEHPHVNIIGHPTARILGRRPPIDFDLDAVFDAAARTGTAMEVNAFPDRLD
                     LRDEHISWARERGVRFAVDTDSHSTLHLRHMRFGVATAQRGWLTRADVINAWPLGRLK
                     RFLAKGRR"
     gene            complement(1974..2894)
                     /gene="argF"
                     /locus_tag="VM638_00940"
     CDS             complement(1974..2894)
                     /gene="argF"
                     /locus_tag="VM638_00940"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HVM11027.1"
                     /translation="MERDFLSIDDLDRAELGQLLDLAAKVKAAPDDHAGRLTGKSVAL
                     IFEKPSTRTRVSFEVGVASLGAHPLALSSRELQLGRGETIEDTGRVLSRYVDCIVLRT
                     FGQERLEQLAAAATIPVVNALSDYEHPCQILADLQTVRERRGDLAGATMTYLGDGNNV
                     AHSLLLGGAMSGMHVRVATPPGYEPIPQVVERSEDLAKETGGSVTVSNDALAAAEGAD
                     ILYTDVWASMGQEAESADRVLVFTPYRLDAEKVRLASDDVLVLHCLPAHRGEEITGEV
                     IDGPHSVVWDQAENRLHAQKALLLWLLGAA"
     gene            complement(2938..5313)
                     /gene="pheT"
                     /locus_tag="VM638_00945"
     CDS             complement(2938..5313)
                     /gene="pheT"
                     /locus_tag="VM638_00945"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HVM11028.1"
                     /translation="MKVPYGWLRELCPTSLSPEEVAAALTGRGIEVDDVRYPWAAVTG
                     VRVARVLDVRDHPGADKLCLATIDTGEGEREVVVGVRNMAPGDLVPHAAPGATLPGFD
                     RPLERRTIRGQVSEGMLCSPKELGISGDHGGILILPSDLEPGMDLARSIGLDDAVIDI
                     EVFPNRPDLLSVLGVARELAAATGDDLHPPDLSVPEAEEEKAGDAAWVEVRDPARCPR
                     YVARVIRGVRHVASPLVAQVRLTAAGMRPLSAVVDATNYVLLELGHPMHPFDLARLAG
                     GGVVVRTAGEGETLRTLDEVERRLSPEDLVIADREKAVGVAGVMGSAAAEVGEGTTDV
                     LLESAWFEPLGILRTARRLGLRTEASVRFERGADPEVPADAAARAARLMVEWAGGIVL
                     AGTVDVGETPPRRTVEVRPRRASLLLGEPLGGAEVREALGRLRLLSREEGDRVQVEVP
                     GWRVDLAREVDVIEEVARVRGYDRLGSALPGVRRAGGLDHRQRLQRRLRDALVRAGLF
                     EVTLPTFVSPAVLDVFEDDRREGIPLRNPVSAEEDRLRTALLPGLLGVARRNAAHRNG
                     SVRLFEIGHVFRTSPLEEEHLAVVLAGDASIGWPSEHRPHDFLDAKGILEHLCEVLGV
                     PRPSLGPPAPLPYHPGRSSMVEVGGRPVGEVGEIHPGVATSFDLSGRVAALELRVDGL
                     LEAAEAAPMYEEVSRFPAVHRDLAFVVGARTPAGRVETILRETSGELLDRVALFDVFE
                     GDPVPAGKRSLAFSLDLRARDRTLTDEEVERVVAAIAERLRAEVGAELRTG"
     gene            complement(5310..6389)
                     /gene="pheS"
                     /locus_tag="VM638_00950"
     CDS             complement(5310..6389)
                     /gene="pheS"
                     /locus_tag="VM638_00950"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HVM11029.1"
                     /translation="MPLDSLGMDVGEALELLRAEQERGLALVGGAGSAEELEHAEVAV
                     LGRRAPISEVQRSLRTLPDEDRPRVGKAVNEVFSTIRAALADRRAVIAAEADRAIAES
                     DRIDVTLPGRRPPLGALHVLTQTEREIVDVFVSLGYRVSEGPELETDWYNFEALNIPP
                     DHPARTMQDTLYVDVPDRPDLVLRTHTSPVQIRTMQAQEPPIYVTIPGRCYRRDVPDP
                     THSPIFHQVEILAVDEGLSMAHMKGTLEAFARAMFGPDQRVRLRPSYFPFVEPGAEVD
                     VSCFVCGGSGCRVCGNGWIEIMGAGMVHPHLFEATGIDPERYTGFAAGMGIERVAMLR
                     HGVPDIRLFYEGDVRFLSQFVRGTA"
     gene            6419..6904
                     /locus_tag="VM638_00955"
     CDS             6419..6904
                     /locus_tag="VM638_00955"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVM11030.1"
                     /translation="MTPRAPRASKDVVILSDVDIDARAEQIWPYLVEWERLDRWMREA
                     SDIRVLGDRREGVGVEAEATVRIAGITTRDVVRVTRWEPPSILEIEHLGWVRGSGYLE
                     LSPLHPRGTNVFWREQLTPPWGRLGRIGMRFLRRSMRRTFAEDLRALRDLVEAEVRPG
                     R"
     gene            complement(6901..8328)
                     /locus_tag="VM638_00960"
     CDS             complement(6901..8328)
                     /locus_tag="VM638_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HVM11031.1"
                     /translation="MITSTANRQVVGLAKLRKRALREDRRRFLVEGAQPCAEALAAGA
                     VETLYHTADAGRHPVVVAASGAGVSVVSVSDQVMTHLTSTVTPQGLLAVARFIDVPLE
                     EVPADAALVPVLCAVRDPGNAGTVLRSADAAGADAVVFAGTSVDVYNPKTVRASAGSL
                     FHLPVVRGAGVEEAVRLLRGRGMRVVAAAADGEESVYDLDLTAPTAVLFGNEAWGIPD
                     EARDLADASVRVPIHGAAESLNLAAAAALVMFESRRQRIAGAPADGGLAAAISASAHD
                     VRSSLTALKGFASMLATRWDRLPEDFRRQIVVGMTVDAEKTAILVKMLVDAARLETGG
                     LNPSPEPTRLEDAAGWVASVFARSDDHPDVEVRGAAAATIDPDRLQGLLLTLTDAVAT
                     LGGQGPVRISISEEPEEASVVIGRDGIEVDEGWAERATDPPRSGAGGGILGVWLAARL
                     AEAQGCSLHAQADPSAPLRLAIPRR"
     gene            complement(8356..8715)
                     /gene="rplT"
                     /locus_tag="VM638_00965"
     CDS             complement(8356..8715)
                     /gene="rplT"
                     /locus_tag="VM638_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVM11032.1"
                     /translation="MPRVKRSVHGRKKRRETLKEAKGYFGARSRRYRVAKEQLLHSGV
                     YAYRDRRRRKGDFRRLWIQRIGAASRQNGMSYSRFMNGLKAAELDIDRKVLADIAVAD
                     PKGFAGLVELARRGPGA"
     gene            complement(8719..8913)
                     /gene="rpmI"
                     /locus_tag="VM638_00970"
     CDS             complement(8719..8913)
                     /gene="rpmI"
                     /locus_tag="VM638_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVM11033.1"
                     /translation="MPKQKTHRGAAKRFRVTRTGKVLHRRANRSHLLGKKTSQRKRRL
                     AGTDEVTAERRGILRLLGRR"
     gene            complement(8915..9445)
                     /gene="infC"
                     /locus_tag="VM638_00975"
     CDS             complement(8915..9445)
                     /gene="infC"
                     /locus_tag="VM638_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVM11034.1"
                     /translation="MNERIRSPQVRLVGPDGAQIGIVPTRDALRQAQEQDLDLVEVAP
                     QADPPVCRIMDYGKYKYERAVRQKEARKRQARIEVKEIKFRPKIDRHDYETKKGHVVR
                     FLEAGARVKVTIMFRGREMAHTELGRKILDRLTEDLTELATVDQTPKQDGRNMVMVLA
                     PVKKAPAKGEKSEKVS"
     gene            complement(9576..9776)
                     /locus_tag="VM638_00980"
     CDS             complement(9576..9776)
                     /locus_tag="VM638_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVM11035.1"
                     /translation="MSTGTVKWFSNEKGYGFISREDGDDIFVHFSAIAGEGYRSLQEG
                     QQVEFDVVDGEKGQQAANVRPV"
     gene            complement(9908..11398)
                     /locus_tag="VM638_00985"
     CDS             complement(9908..11398)
                     /locus_tag="VM638_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11036.1"
                     /translation="MRRPALIAALLLGGSLLPATATADQLVVKQDLGSGPISTDNVTY
                     HGTIPIDSPGVGGEVVVRPDLGNKKFFYATGLKGLSIYDVTNPERPVPAGHFPFAHAQ
                     NEDVKVSDDGRTVVIAADGALLVGINPVTTGITVIDVTNPALPRLVASSNDRVMGRGT
                     AAGLAEHTAECADATCSVIYGRTGRIYKVDARLGRVDPVGTWTRDLNGVNVSGIHALN
                     RDESGLIISDSTTRLILDPGVLDPQATPLKPKVLAQGNRASQDNRLQHNNVRTDALDW
                     VPRPRPEDPEEEEIIPTAEAGVDFPVSPRSVSIQRVREIMEPGELVIGNSESNLNTFC
                     STAGGLSTWGIVNFDREERTTIQQLEVFRPLNGTWADGSPAANALGCSGHWFTEGDGV
                     VAASWYEHGVRFFEIDKRYGTINEVGYFQPVATQAGAAYWIDDTYVYSVDYARGIDIL
                     SFDRDADPATQAELDANWIAGLSLVGSGTFAEQERYACRQAALKVG"
     gene            complement(11524..11874)
                     /locus_tag="VM638_00990"
     CDS             complement(11524..11874)
                     /locus_tag="VM638_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVM11037.1"
                     /translation="MIVEKPWGKVTTYALNQPSSVRVIAIEPGQETSEHYHQLRDEMW
                     VVLDEGLRIQIGNRVIEAKPGDEFVVQAEEPHRISNEGPKRGRVLEVAYGYTTEDDTF
                     RLQDDYGRSLDPDW"
     gene            complement(11879..>12003)
                     /locus_tag="VM638_00995"
     CDS             complement(11879..>12003)
                     /locus_tag="VM638_00995"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVM11038.1"
                     /translation="ETLEQALDRAGGKQGNKGWEAACSAIEMANLLEALPKEEG"
BASE COUNT         1641 a         4346 c         4267 g         1749 t
ORIGIN      
        1 ggacgtcgct ccgtctcgcg cgggacctgc tcggacgggt gctggtacgc gacctgggcg
       61 gcacccggct ggccgggcgg atcgtcgagg tcgaggccta ccggcccgac gacccggcca
      121 gccgggtgcc gcccaggtcg cgtaccagca cccgtccgag caggtcccgc gcgagacgga
      181 gcgacgtccg ttcgaagaac gcccgcggca gcgcctcgag gtcgtcgctc agcgtcgtcc
      241 tttcgcgagg aaccgcttga gccggccgag cggccaggcg ttgatcacat cggcacgggt
      301 gagccagccg cgttgagcgg tcgctacccc gaagcgcatg tgccgcaggt gcagggtcga
      361 gtgcgagtcg gtgtcgacgg cgaagcggac gcctcgctcc cgggcccagg agatgtgctc
      421 gtcgcggaga tccaggcggt cggggaaggc gttgacctcc atcgcggtcc cggtgcgcgc
      481 cgcggcgtcg aagaccgcgt cgaggtcgaa atcgatcggc ggccgccggc cgaggatgcg
      541 ggccgtcggg tggccgatga tgttgacgtg gggatgctcc atcgcccgga tgatgcgccg
      601 cgtcatctcc tccttcgact ggttgaagtg cgagtgcacg ctcgcgacga ggacgtcgaa
      661 cccggagagg aactcctcgt cccagtcgac cgagccgtcg ggctggatgt tcagctcgga
      721 tccgtgcagg agcgtcatcg ccgggtagcg cggctggagc tcggcgaggc gacgtcgctg
      781 cgccagcgcc ttctcgcgcg tcatccgctg catgaacagc agctccgcgt ggtcggtgac
      841 ggcgtagtag gcgtagccgc gctccgcggc ggcgtcgagc atcttctcga ggctcgcgag
      901 cccgtcggtg agatcggtgt gcgtgtggag gtcgcccttg aggtgccgct cctggaggag
      961 gtcgggcatc tcgccggcaa ccgccgcctc gacctccccg cgatcctcgc gcagggtcgg
     1021 cgggatccac gggagaccga ggcgctcgta gatctcctcc tccgtctcgg ccacgaggcg
     1081 ccggccgctc ttcacgcgga agagcccgta ctccgagagc ttcaggccct tgcgaaccgc
     1141 catctcacgg atccggacgt tgtgcgcctt cgagccggtg aagtactgca tcgccgcgcc
     1201 ccagacggcc ggctcgacga cgcggaggtc gacctggaga cccttggtcg tgcggacgga
     1261 gcatttcgtg gggccccgtg cgatcacctc ctggacgagg tcgagggacg cgaaggcgtc
     1321 catcacctcc tcggcgccct ccgaagcgac gaggagatcg atgtcgccga tcgtctcgcg
     1381 catccgccgc agcgacccgg cgtagccggc gcggccgacg gcccggttct gccgcagctc
     1441 cgcgaggagc tcttcggcga ggtcgagcgc gacccccagc gggacccggt cccccgcctg
     1501 ctgcatcagc tcgacgcccc gcaggatgtt ctcctccgtc cgggccccga agcccttcag
     1561 gtcccggagg cgcccggcgt ggagcgcgtc gaggagctcc tccacgctgg agatgcccag
     1621 ctcgtcgaag aggacgaggg ctttcttcgg cccgagcccc gggatcgcga ggagcgaccg
     1681 gacgccgtcg gggatgtccg cacgggcttc ctccagcgca cggaaggtgc ccgtgcgcag
     1741 gatctcgacg accttctccg cgatcgaccg cccgacgttg gggatcgcga ggatcccctt
     1801 caggtcgagc ttcgcgacgt cgccgccgtg gccgccgatc gcccgggcgg ccttctcgta
     1861 ggcgcgagcc ttgaaggcgt cgccccccga gatcgagatg aggtcggagt actcctgcag
     1921 gagcgcctcg acctcgtcgt tcgcccgggg catccgctag gcggcggggg cgctcatgcg
     1981 gcgccgagca gccacagcag gagcgccttc tgcgcgtgga gccggttctc ggcctggtcc
     2041 cagacgaccg agtgcggccc gtcgatcacc tcgccggtga tctcctcacc ccggtgggcg
     2101 gggaggcagt ggagcacgag gacgtcatcg ctcgcgaggc ggaccttctc cgcgtccaac
     2161 cggtacggcg tgaagaccag cacgcggtcc gccgattccg cctcctgacc catcgaggcc
     2221 cagacgtcgg tgtagaggat gtccgcgcct tccgccgcgg cgagcgcgtc gttcgagacg
     2281 gtgacgcttc cgcccgtttc cttcgcgagg tcctcgctgc gctcgaccac ctgggggatc
     2341 ggctcgtacc ccggcggggt cgcgacacgg acgtgcatcc cgctcatcgc gccgcccagc
     2401 agcagcgagt gggcgacgtt gttcccgtcc ccgagatagg tcatggtcgc cccggccagg
     2461 tccccacgac gctcccgaac ggtctggagg tcggccagga tctggcacgg gtgctcgtag
     2521 tcggagagcg cgttgacgac cgggatggtg gcggcggccg cgagctgctc gagccgctcc
     2581 tggccgaagg tccgcagaac gatgcagtcg acgtagcggg agaggacgcg gccggtgtcc
     2641 tcgatcgtct cgccgcggcc gagctgaagc tcgcgcgacg agagcgcgag ggggtgcgcg
     2701 ccgaggctcg ccacgcccac ctcgaacgag acgcgcgtcc gcgtggatgg cttctcgaag
     2761 atgagcgcga cgctcttccc ggtgagccgc ccggcgtggt cgtccggggc ggccttcacc
     2821 ttcgcggcga ggtcgagcag ctgcccgagc tcggcgcggt ccaggtcgtc gatcgaaagg
     2881 aagtcccgct ccacgtggcg gagcgtaccc gagccctcgg tgccgggcaa cggcgggtca
     2941 gcccgtccgc agctcggcgc cgacttctgc ccggagccgc tcggcgatgg ccgccacgac
     3001 gcgctcgacc tcctcgtccg tcagcgtccg gtcgcgggcg cggagatcga gcgagaacgc
     3061 gagggagcgc ttgccggcgg gcaccggatc gccctcgaac acgtcgaaga gcgcgacgcg
     3121 gtccaggagc tcccccgacg tctcgcggag gatcgtctcg acccggccgg cgggcgtgcg
     3181 agcacccacc acgaaggcga ggtcccgatg gacggccggg aagcgtgaga cctcctcgta
     3241 catcggggcg gcttcggccg cctcgaggag gccgtcgacc cggagctcga gcgcggcgac
     3301 gcgcccgctg aggtcgaacg acgtcgcgac cccgggatgg atctcgccca cctcgccgac
     3361 cggccgcccg ccgacctcga ccatcgagga acggcccggg tggtacggga gcggcgccgg
     3421 cggcccgagc gatggacggg gcacgccgag gacctcgcag aggtgctcga ggatgccctt
     3481 cgcgtccagg aagtcgtgcg gacggtgctc ggagggccac ccgatggacg cgtcgcccgc
     3541 cagcacgacg gccaggtgct cctcctcgag cggagatgtt cggaacacgt ggccgatctc
     3601 gaagagacgg acggacccgt tgcggtgagc ggcgttccgc cgtgccaccc ccagcagccc
     3661 cggcagcagc gcggtccgca gccggtcctc ctcggccgac acggggttgc ggagggggat
     3721 gccctcgcgc cggtcgtcct cgaagacgtc gaggaccgcc ggggagacga aggtcgggag
     3781 cgtcacctcg aagagccccg cgcgcaccag cgcgtcgcgg aggcggcgct gcagacgctg
     3841 tcggtggtcg aggccccccg cccggcggac cccgggcaac gccgatccga ggcggtcgta
     3901 gccgcggacc cgggcgacct cctcgatcac gtcgacctcc ctggccagat cgacccgcca
     3961 tcccggcacc tcgacctgga cgcgatcgcc ctcctcccgc gagagcaggc gcaggcgccc
     4021 gagcgcctcc cgcacctcgg ctccgccgag cggctccccg agcaggaggg aggcccgccg
     4081 gggccggacc tcgaccgtgc gacgtggcgg cgtctcgccg acgtcgaccg tccccgcgag
     4141 cacgatcccg ccggcccact ccaccatgag acgtgccgca cgggccgcgg catccgccgg
     4201 aacctcaggg tcggcgccgc gctcgaaccg cacggaggct tcggtccgca ggccgagacg
     4261 gcgcgcggtc cggaggatcc cgagaggctc gaaccaggcg ctctcgagca ggacatccgt
     4321 cgtcccctca ccgacctccg ccgcagccga acccatgacg ccggccacgc ccaccgcctt
     4381 ctcccggtcc gcgatcacca ggtcctccgg gctcagcctg cgctccacct cgtcgagggt
     4441 ccgcagggtc tctccctcgc cggcggtccg caccaccacg ccaccccccg cgaggcgcgc
     4501 gaggtcgaag gggtgcatcg gatgcccgag ctcgaggagg acgtagttcg tcgcgtcgac
     4561 gacggccgag agcggccgca tcccggcggc cgtgagccga acctgggcga cgaggggcga
     4621 cgcgacgtgc cgcacccccc ggatcacccg cgccacgtag cgagggcacc gggcggggtc
     4681 gcggacctcg acccacgctg catcaccggc cttctcctct tctgcctccg gcacgctcag
     4741 gtccggcgga tgcaggtcgt cgcccgtcgc ggcggccagc tcccgagcga ccccgagcac
     4801 cgacaggagg tccgggcgat tggggaacac ctcgatgtcg atcaccgcgt cgtccaagcc
     4861 gatcgagcgc gcgagatcca tcccgggctc cagatcggat ggcaggatca ggatccctcc
     4921 gtggtcgccc gagatgccga gctccttcgg cgagcagagc atcccttcgg agacctggcc
     4981 gcggatcgtc cggcgctcca gaggacggtc gaagcccggc agcgtcgcac ccggcgccgc
     5041 gtgcgggacg aggtcgcccg gcgccatgtt ccgcacgccg acgaccacct cgcgctcgcc
     5101 ctcgccggtg tcgatcgtcg ccaggcagag cttgtcggcg ccggggtggt cgcggacgtc
     5161 gagcacgcgc gcgacgcgga cgccggtcac cgccgcccac gggtagcgga cgtcgtcgac
     5221 ctcgatcccc cgcccggtga gcgcggcagc aacctcctcg ggtgagagcg aggtcggaca
     5281 cagctcgcgg agccagccgt aggggacctt cacgcggtcc cccggacgaa ctgggagagg
     5341 aagcggacgt cgccctcgta gaagagccgg atgtcgggga ccccgtggcg cagcatggcc
     5401 acgcgctcga tccccatccc cgccgcgaac cccgtgtagc gctccgggtc gatgcccgtc
     5461 gcctcgaaca ggtgcgggtg gaccatcccg gcgcccatga tctcgatcca gccgttgccg
     5521 cagacccggc agccgctgcc gccgcagacg aagcaggaga catcgacctc ggcacccggc
     5581 tcgacgaacg ggaagtacga gggccgcagg cgcacgcgct ggtcggggcc gaacatcgcg
     5641 cgcgcgaacg cctcgagcgt ccccttcatg tgggccatcg agagtccctc gtcgaccgcg
     5701 aggatctcga cctggtggaa gatcggcgag tgcgtcggat ccgggacgtc gcgccggtag
     5761 caccgcccgg ggatcgtcac gtagatcggc ggctcctgcg cctgcatcgt gcggatctgc
     5821 acgggcgagg tgtgggtgcg caggacgagg tcggggcggt cgggcacgtc cacgtacaaa
     5881 gtgtcctgca tcgtccgggc ggggtggtcc ggcgggatgt tgagcgcctc gaagttgtac
     5941 cagtcggtct cgagctccgg cccctcgctc acgcggtagc cgagcgagac gaagacgtcg
     6001 acgatctcgc gctcggtctg cgtgagcacg tggagcgcgc cgagcggcgg ccggcggccc
     6061 ggcagcgtga cgtcgatccg gtcgctctcg gcgatcgccc gatcggcctc ggccgcgatc
     6121 accgcgcgtc ggtccgcgag ggccgcacgg atggtggaga acacctcgtt caccgccttc
     6181 ccgacccgcg gccggtcctc gtctgggagc gtccgcaggg accgctggac ctccgagatc
     6241 ggcgcgcgcc ggccgagcac cgcgacctcg gcgtgttcga gctcctcggc gctgcccgcc
     6301 cctcccacca gggcgagccc tcgctcctgc tccgcccgca gcagctccag cgcctcgccg
     6361 acgtccatgc cgagggagtc tagcggcatc ccctcgagcg gcgacgtatc ctccgtccgt
     6421 gaccccgagg gccccccgcg cgagcaagga cgtcgtgatc ctcagcgacg tggacatcga
     6481 cgcgcgggcg gagcagatct ggccgtacct cgtcgagtgg gagcggctgg atcgctggat
     6541 gcgggaggcc agcgacatcc gggtgctcgg tgaccgacgc gaaggcgtcg gggtcgaggc
     6601 ggaagccacc gtgcggatcg cggggatcac gacccgggac gtcgtgcggg tcacccgatg
     6661 ggagccgccc tcgatcctcg agatcgagca cctcggctgg gtgcgcggct cgggctacct
     6721 cgagctctcc ccgctccacc cgcggggcac gaacgtcttc tggcgcgagc agctgacccc
     6781 cccgtgggga cgcctcggac gcatcgggat gaggttcctg cggcgctcca tgcgccgcac
     6841 cttcgccgag gacctccggg cgctccgcga cctcgtcgag gcggaggtcc gtccgggccg
     6901 ctaacggcgc gggatcgcga ggcggagggg cgccgagggg tccgcctggg cgtggagcga
     6961 acacccctgg gcctcggcca gtcgcgcggc gagccagacg ccgaggatgc cgccgccggc
     7021 gcccgaccgc gggggatccg tcgcgcgctc ggcccagccc tcgtcgacct cgatcccgtc
     7081 gcgcccgatc acgacgctcg cttcctccgg ctcctcggag atcgagatgc ggaccggacc
     7141 ctggccaccg agcgtcgcga ccgcgtccgt gagggtcagg agcagcccct ggaggcggtc
     7201 gggatcgatc gtggcggcgg ccgctcctcg cacctcgacg tcggggtgat cgtcgctgcg
     7261 cgcgaagacc gacgccaccc agcccgcggc atcttccaga cgggtgggct ccggggaggg
     7321 gttgaggcct ccggtctcga ggcgggccgc gtcgacgagc atcttcacga ggatcgccgt
     7381 cttctcggcg tcgaccgtca tcccgacgac gatctgccgg cggaagtcct cggggagacg
     7441 atcccatcgg gtcgcgagca tcgaggcgaa gcccttcagc gcggtgagcg atgagcggac
     7501 atcgtgggcc gaggcggaga tcgcggccgc gagcccgccg tcggccggtg ccccggcgat
     7561 gcgctggcgt ctcgactcga acatcacgag cgccgccgcg gccgcgaggt tcaacgactc
     7621 ggcggcgccg tggatcggga cgcggacgct cgcgtcggcg agatcgcgcg cctcgtccgg
     7681 gatcccccag gcctcgttgc cgaacaggac cgccgtcggc gcggtcagat cgaggtcgta
     7741 gacgctctct tccccgtcgg cggcggcggc caccacgcgc atcccgcggc cgcgcaggag
     7801 cctgaccgct tcctcgacgc ccgctcctcg cacgaccggg aggtggaaga gcgagcccgc
     7861 ggacgcgcgg acggtcttgg ggttgtacac gtcgacggac gtgccggcga agacgaccgc
     7921 gtcggccccg gcggcatccg ccgagcggag gacggtgccc gcgttcccag ggtcgcggac
     7981 ggcgcacagg accggaacga gtgcggcatc ggcggggacc tcctcgagcg ggacgtcgat
     8041 gaaccgcgcg accgcgagca ggccctgggg cgtgaccgtc gaggtgaggt gggtcatcac
     8101 ctggtcggag acggagacga cgctgacgcc ggcgccgctc gccgcgacga ccaccgggtg
     8161 ccggccggcg tcggccgtgt ggtagagggt ctcgaccgcg ccggcggcga gcgcctccgc
     8221 gcacggctgg gctccctcga ccaggaaacg gcggcgatcc tcccgcagcg cgcgcttgcg
     8281 gagcttcgcg agcccgacga cctggcggtt agcggtgctg gtgatcacgg cgaacccgcc
     8341 gcgcgggtca ccgccctagg cgccggggcc cctgcgggcg agctcgacga ggccggcgaa
     8401 gcccttcggg tcggcgaccg cgatgtcggc gaggaccttc cggtcgatgt cgagctccgc
     8461 cgccttgagg ccgttcatga accgcgagta ggacatgccg ttctgacgcg acgcggcgcc
     8521 gatccgctgg atccagaggc ggcggaagtc gcccttgcgg cggcggcggt cgcggtacgc
     8581 gtagacgccg gagtgcagga gctgctcctt ggccacgcgg taccggcgtg agcgcgcgcc
     8641 gaagtagccc ttcgcctcct tcagggtctc gcggcgcttc ttccggccgt ggacgctgcg
     8701 cttgacgcgg ggcatcgcct atcgcctccc gaggagccgc aggatcccgc ggcgctccgc
     8761 ggtcacctcg tcggtgccgg ccaggcggcg cttccgctgt gacgtcttct tccccagcag
     8821 gtgcgagcgg ttcgcccgac ggtgcaggac cttgccggtc ctcgtcaccc ggaagcgctt
     8881 cgccgcgccg cggtgggtct tctgcttcgg catctcagct caccttctcg ctcttctcgc
     8941 ccttcgcggg cgccttcttg accggggcca ggaccatgac catgttgcgg ccgtcctgct
     9001 tcggcgtctg gtccaccgtc gccagctccg tcaggtcctc ggtcagccga tcgaggatct
     9061 tcctcccgag ctcggtgtgg gccatctcgc gtccacggaa catgatcgtc accttcacgc
     9121 gcgcgccggc ctccaggaac cggacgacgt gccccttctt cgtctcgtag tcgtggcggt
     9181 cgatcttcgg ccggaacttg atctccttga cctcgatcct cgcctgccgc ttgcgcgcct
     9241 ccttctggcg gaccgcccgc tcgtacttgt acttgccgta gtccatgatc cggcacaccg
     9301 gcgggtcggc ctgcggagcg acctcgacca ggtcgaggtc ctgctcctgc gcctggcgca
     9361 gggcgtcccg cgtcggcacg atcccgatct gggcgccatc gggtccaacc aaccggacct
     9421 gcggcgaccg gatccgctcg ttgatgcgcg gctcaaccga gaccgcggat cacctccatc
     9481 gaagcggaag gcgcgtcacg agcggtgatg agcggatcgg accatggacc accgagtata
     9541 gggggtgctc gaacggggtg tcgacgagcc caacctcaca cggggcggac gttcgccgcc
     9601 tgctggccct tctcaccgtc gacgacgtcg aactcgacct gctgcccttc ctgcagggag
     9661 cggtatccct cgccggcgat cgccgagaag tgcacgaaga tgtcgtcgcc atcctcacgg
     9721 gagatgaagc cgtagccctt ctcgttgctg aaccacttga ccgttcccgt gctcacctcg
     9781 tcctccttgc tcatggcggc gggccgcccg atccgaagcg agcgtattgg aacccatcgg
     9841 ggcgcttgcg gtcaacaccg ggcgagccgg gagggggccg ccgatcgcga ccccctcctc
     9901 ggcccgctca gccgaccttc agcgcggcct gccggcaggc gtagcgctcc tgctcggcga
     9961 aggtcccgga tcccacgagg ctgaggccgg cgatccagtt cgcgtcgagc tcggcctggg
    10021 tcgcgggatc ggcgtctcgg tcgaagctca ggatgtcgat cccccgggcg tagtcgacgg
    10081 agtacacgta ggtgtcgtcg atccagtacg ccgctccggc ctgggtcgcg accggctgga
    10141 agtacccgac ctcgttgatc gtcccgtagc gcttgtcgat ctcgaagaag cggacgccgt
    10201 gctcgtacca gctcgcggcc acgaccccgt cgccctcggt gaaccagtga ccggagcagc
    10261 cgagcgcgtt cgccgcggga ctgccgtcgg cccaggtccc gttcagcggc cggaacacct
    10321 cgagctgctg gatcgtcgtc cgctcctcgc ggtcgaagtt cacgatgccc caggtcgaga
    10381 gcccaccggc cgtcgagcag aaggtgttga ggttgctctc ggagttgccg atcaccagct
    10441 ctccgggctc catgatctcc cggacccgct ggatcgagac cgaccgcggg ctcaccggga
    10501 agtccacgcc tgcctccgcc gtggggatga tctcctcttc ctccgggtcc tcggggcgcg
    10561 gccgcggcac ccagtcgagg gcgtcggtgc gcacgttgtt gtgctgcagg cggttgtcct
    10621 gcgacgcgcg gttcccctgc gccaggacct tcggcttcag cggggtggcc tgcgggtcga
    10681 ggacgccggg gtcgaggatc aggcgggtcg tcgagtcgct gatgatgagc ccgctctcgt
    10741 cgcggttcag ggcgtggatc cccgagacgt tgacgccgtt gaggtcccgg gtccacgtgc
    10801 cgaccggatc cacacggccc aggcgggcgt cgaccttgta gatccggccg gtccgtccgt
    10861 agatcaccga gcacgtcgcg tccgcgcact ccgcggtgtg ctccgcgagg ccggcggcgg
    10921 tgccgcgtcc catcacgcgg tcgttcgagg aggcgaccag gcgcggcaag gccgggttcg
    10981 tcacgtcgat cacggtgatg cccgtcgtga ccgggttgat cccgaccagc agggcgccgt
    11041 cggcggcgat cacgacggtc cgcccgtcgt cgctcacctt cacgtcctcg ttctgcgcgt
    11101 gcgcgaacgg gaagtgcccg gcggggaccg gccgctcggg gttcgtcacg tcgtagatcg
    11161 acaggccctt gaggccggtc gcgtagaaga acttcttgtt gccgaggtcg ggccggacga
    11221 cgacctcgcc acccaccccc ggggagtcga tcgggatcgt cccgtggtac gtcacgttgt
    11281 cggtcgagat cgggccggag ccgagatcct gcttgacgac gagctggtcg gccgtcgccg
    11341 tggccggcag gagggaccct ccgagcagga gggcggcgat cagggctggt ctacgcatct
    11401 gcggcacctc tctggtcgcg gcccgcatcc gacgcgggcc cttctccgga gaggttcggc
    11461 gtcagcgaga ccccaccctt tgtgcagatg cacgaaaccg ggggatcggc ggacggtccg
    11521 ccactaccag tcgggatcga ggctgcgccc gtagtcgtcc tggaggcgga aggtgtcgtc
    11581 ctcggtcgtg tacccgtagg cgacctcgag gacgcggccg cgcttcgggc cctcgttgga
    11641 gatccggtgc ggctcctcgg cctggacgac gaattcgtcg ccgggcttcg cctcgatcac
    11701 gcggttgccg atctggatcc gcagcccctc atcgaggacc acccacatct cgtcgcggag
    11761 ctggtggtag tgctccgatg tctcctggcc cggctcgatc gcgatgacgc ggaccgagga
    11821 gggctggttc agcgcgtagg tcgtcacctt cccccagggc ttctcgacga tcatccgatc
    11881 acccctcctc cttcggcagc gcctccagca ggttcgccat ctcgatcgcg gagcaggccg
    11941 cctcccaccc cttgttcccc tgcttcccgc cggcccggtc gagcgcctgc tcgagcgtct
    12001 cgg
//
LOCUS       DATLGG010000039         2620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_19314, whole genome shotgun sequence.
ACCESSION   DATLGG010000039 DATLGG010000000
VERSION     DATLGG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19314"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VM638_01000"
     CDS             complement(<1..900)
                     /locus_tag="VM638_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVM11039.1"
                     /translation="MVSLLGHRDEFLKIVEAAFDLQILVRGNEITIGGEGEEVDRAAQ
                     IFEDLLLLLERGQNLTPEAIHQTIGILKRDEGRPADVLGDVVISSRGTSIAPKTVGQK
                     RYVDAIRRSTVTFSVGPAGTGKTYLAVASAVKALQERKISRIILTRPAVEAGERLGFL
                     PGTLYEKIDPYLKPLYDALYDMMDADSFQRLMQRGTIEVAPLAYMRGRTLNSSFIILD
                     EAQNTSPEQMQMFLTRLGFGSKVVVTGDVTQIDLPRDQASGLIQVRDILSSIDGIGFV
                     EFSHQDVVRHKLVQRIVEAYKLHA"
     gene            complement(1027..1371)
                     /locus_tag="VM638_01005"
     CDS             complement(1027..1371)
                     /locus_tag="VM638_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HVM11040.1"
                     /translation="MATCLFCRIVAKEVEADIVHESDDVLAFRDIDPVAPTHILVIPK
                     EHIDSARAIRDRHAELAAELFQVAAHLARADGIDRTGWRLVTNIGDDAGQSVHHLHIH
                     LLGGRRLGWPPG"
     gene            complement(1379..2098)
                     /locus_tag="VM638_01010"
     CDS             complement(1379..2098)
                     /locus_tag="VM638_01010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HVM11041.1"
                     /translation="MSLPRFFAGAPEVGGMVVLEGDEARHAAGARRLRDGDMLESGDG
                     EGAIATCVVEASDARRVVARVQERRVEPRPAPHVTVALAPPKGDRLTWGLQKLTELGV
                     DRILLLETERTVRHPAGLERLRRVVREAAKQSGRPYLPPVDGPVPWTEALEAAPVGTL
                     SCVLYEEADDGLLGLLPQERPARVLILVGPEGGLTQEEASGGAERDALLASLGPTLLR
                     TETAAIVGASLVLARYGRLGR"
     gene            complement(2095..>2620)
                     /locus_tag="VM638_01015"
     CDS             complement(2095..>2620)
                     /locus_tag="VM638_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HVM11042.1"
                     /translation="VIPDPCDRCGGNGLEARVDDVTVEIPPGVDDGIEVRVEARGDDG
                     PRGARPGDLLLSLRVEPHLVFERRGADLAATLPVPFTQAILGADLEVDTIHGPETVRI
                     APGTPSGTVLRVRGGGVPHLQRRGRGDLYLQVEVELPASLPKRERALLEQLAELRGEE
                     SRGGPSRATLRRRS"
BASE COUNT          365 a          910 c          961 g          384 t
ORIGIN      
        1 ggcgtggagc ttgtacgcct cgacgatccg ctggacgagc ttgtgccgga cgacgtcctg
       61 gtgcgagaac tccacgaagc cgatcccgtc gatcgagctg aggatgtccc gcacctggat
      121 cagcccggac gcctggtcgc ggggcaggtc gatctgggtc acgtcgccgg tgacgacgac
      181 cttcgagccg aagccgagcc gggtcaagaa catctgcatc tgctccggcg aggtgttctg
      241 cgcctcgtcg aggatgatga acgacgagtt cagggtgcgc cctcgcatgt acgcgagcgg
      301 cgcgacctcg atcgtccccc gctgcatcag gcgctggaac gagtccgcgt ccatcatgtc
      361 gtagagcgcg tcgtacagcg gcttgaggta cggatcgatc ttctcgtaga gcgtgccggg
      421 caggaagccg agccgctccc cggcctcgac cgccggacgg gtcaggatga tccgggagat
      481 cttgcgctcc tgcagggcct tcaccgcgct cgcgacggcg aggtaggtct tccccgtccc
      541 ggcgggcccg accgagaagg tcacggtgct ccggcggatc gcgtcgacgt agcgcttctg
      601 cccgacggtc ttcggagcga tcgatgtccc gcgggagctg atcacgacgt cgccgaggac
      661 gtccgcgggg cgcccctcgt cgcgcttcag gatcccgatc gtctgatgga tggcctccgg
      721 ggtgaggttc tgcccgcgct cgagcagcag gaggaggtcc tcgaagatct gcgccgcgcg
      781 gtcgacctcc tctccctcgc ccccgatcgt gatctcgttg ccgcggacca ggatctggag
      841 atcgaacgcc gcttcgacga tcttcaggaa ctcgtcgcga tgtccgagga ggctgaccat
      901 cgggcggttg cccgggacca ggatcttgac ctgcttcgtg gccttcgcca agtcgtctat
      961 ctcgctttgg gaaaaggggc cgcctgggcg accccgctgc gcgcgattat acccaccagc
     1021 ctgcggttat cccgggggcc atccgaggcg ccgaccgccg aggaggtgga tgtggaggtg
     1081 atggacgctc tgccccgcgt cgtcgccgat gttcgtgacg agccgccatc cggtgcggtc
     1141 gatgccgtcg gcccgggcga ggtgcgccgc gacctggaac agctctgcgg caagctcggc
     1201 gtgacggtcg cggatcgcgc gggcggagtc gatgtgctcc ttcgggatca cgaggatgtg
     1261 ggtcggcgct acggggtcga tgtcgcggaa ggcgaggacg tcgtcggact cgtggacgat
     1321 gtcagcctcc acctccttcg ccacgatgcg gcagaacagg caggttgcca tgggcggatt
     1381 atctcccgag acgcccgtag cgggcgagga ccagcgaagc cccgacgatc gccgccgtct
     1441 cggtccgcag gagggtcggc ccgagcgagg caaggagcgc gtcacgctcg gcgccgccgg
     1501 acgcctcctc ctgcgtgaga ccgccctcgg ggccgacgag gatgagcact cgcgcaggcc
     1561 gttcctgagg caggaggccg aggaggccgt cgtccgcctc ctcgtagagg acgcacgaga
     1621 gcgtgccgac cggcgccgcc tcgagcgcct cggtccatgg gaccgggcca tcgaccggtg
     1681 ggagataggg ccggcccgac tgcttcgccg cttcgcggac gacgcggcgg agacgctcga
     1741 ggcccgcggg atgacgaacg gtgcgctcgg tctcgagcag caggatccgg tcgaccccga
     1801 gctcggtcag cttctggagc ccccaggtga gccggtcgcc cttcggcgga gcgagcgcga
     1861 cggtcacgtg cggagcgggc ctcggctcca cgcgccgctc ctgcacgcgg gcgacgaccc
     1921 ggcgggcgtc cgacgcctcc acgacgcagg tggcgatggc cccctcgccg tccccggact
     1981 cgagcatgtc cccgtcacgc agccgccggg cccccgccgc gtgccgcgcc tcgtcgccct
     2041 cgaggacgac catcccgccg acctcggggg cccccgcgaa gaaccgcggc aggctcatga
     2101 ccggcggcgg agcgtggccc gcgagggccc gccgcgggat tcctcccctc ggagctcggc
     2161 gagctgctcg agcagggcgc gctcgcgctt ggggagggag gcgggaagct cgacctcgac
     2221 ctggaggtag aggtcccctc ggccgcgccg ttgcaggtgc gggacgcccc cgccgcggac
     2281 ccggagcacg gtgccggagg gagtgccggg cgcgatgcgg accgtctccg ggccgtggat
     2341 cgtgtcgacc tcgaggtcgg cgccgaggat cgcctgggtg aacggaaccg ggagcgtggc
     2401 ggccaggtcg gctccccggc gctcgaacac caggtgcggt tcgacccgca gcgagaggag
     2461 gagatcgccg ggccgtgccc cccgcggccc gtcatcgccg cgcgcttcga cgcgcacctc
     2521 gatcccgtcg tcgacccccg gggggatctc gacggtgacg tcgtcgacgc gcgcctcgag
     2581 gccgttcccg ccgcagcggt cgcaggggtc ggggatgacc
//
LOCUS       DATLGG010000040         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_129813, whole genome shotgun sequence.
ACCESSION   DATLGG010000040 DATLGG010000000
VERSION     DATLGG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129813"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VM638_01020"
     CDS             <1..699
                     /locus_tag="VM638_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11043.1"
                     /translation="LLVVIFALTAFPLGPEWDREELGAFARPAALLVGAAVVVTVAWV
                     LVRRRPASGSAPSAIGPIAAIAAAAVLIGSVAAGGGLRVEGDRCPRGPLSAYLSTLPK
                     DAVIAGDPVDMTCVPLVAQRPVVMSRKLYGVRHRDRMRDLVDAYYGGSVEAILRLRER
                     YGAEHLVVRPDLLLARDLTGPWRRMEPFTSQRRRLMRSVPRPAALELPIKCRTYEGPR
                     AHVVDLACVERELG"
     gene            complement(696..1226)
                     /locus_tag="VM638_01025"
     CDS             complement(696..1226)
                     /locus_tag="VM638_01025"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVM11044.1"
                     /translation="MIQGSAGPVDLTPKPVSASRIVLAQIMQVADANIAGNVHGGTIM
                     KLVDNAAGLAAAKHAGGLAVTAVMDEMSFLQPVYLGDLVTLKASVNEAFTTSMEVGVR
                     VESESLTTGRVTHTSSAYLVFVAVDEHGQPRKVPPLLAETDEERQRQREAALRRQARL
                     ERKEAIERARSRERSS"
     gene            complement(1223..2383)
                     /locus_tag="VM638_01030"
     CDS             complement(1223..2383)
                     /locus_tag="VM638_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM11045.1"
                     /translation="MDLFNLPEELEEFRGLVREICEERLASRAADIDEEDEFPWDVHK
                     LFVENELMAVGYPEEHGGAGGGSLTMAVMVEEIARISASASLIPLVNRLGAIPVLLAG
                     SDEQKTALFGGISRGDNQMSYALTEPGAGSDAGSLQTRYERTDGGFRLHGTKRFITGA
                     GASDRYTVFATKDPSMRTKGISAFIVHSDDEGFSVGKSEKKLGIRGSPTREVYLDNVE
                     IPEDRLLGEEGKGFTYAMQTLDYSRPTIAAQALGIAQGAFDVASRYLTEREQFGKKIA
                     EFQGLQFMLADMAMQVEAARMLTYRAAAATDAKDPKMTYHAAVAKCHASDTAMAVTTD
                     AVQMLGGYGYMREYGVERMMRDAKITQIYEGTNQVQRIVIARQVLRSIGAAG"
BASE COUNT          339 a          945 c          903 g          437 t
ORIGIN      
        1 ctcctcgtgg tgatcttcgc gctcaccgcg ttcccgctcg gacccgagtg ggaccgcgag
       61 gagctcggcg cgttcgcgcg gccggctgcg ctcctcgtgg gggccgccgt cgtggtcacg
      121 gtcgcgtggg tgctcgtgcg ccgccgcccc gcctcggggt cggcaccgag cgccatcggg
      181 ccgatcgcgg cgatcgcggc ggcggcggtc ctgatcggat cggtggccgc cggcggaggg
      241 ctgcgggtcg agggggaccg ttgcccgcgg gggccgctct cggcctacct ctcgacgttg
      301 ccgaaggatg ccgtcatcgc cggcgatccc gtcgacatga cctgcgtccc gctcgtcgcc
      361 cagcggccgg tcgtgatgtc caggaagctc tacggggtcc gtcaccgcga ccggatgcgg
      421 gacctcgtcg acgcgtacta cggcggctcg gtcgaggcga tcctgcgcct gcgcgagcgc
      481 tacggcgccg aacacctcgt cgtgcggccg gacctcctgc tcgcccgcga cctcaccggc
      541 ccctggcgac ggatggagcc gttcacgtcg cagcggcggc ggctgatgcg gtcggttccg
      601 cgcccggcgg cactcgagct tcccatcaag tgccggacct acgagggacc gcgcgcccac
      661 gtcgtcgatc tcgcctgcgt cgagcgcgag ctgggctagg agcttctctc ccgggagcgc
      721 gcccgctcga tcgcctcttt ccgctcgagc cgtgcctgtc ggcgcaacgc cgcctcgcgc
      781 tgccgctggc gctcctcgtc ggtctccgcg agcaacggcg ggaccttccg gggctggccg
      841 tgctcgtcga ccgcgacgaa gacgaggtac gcgctcgagg tgtgcgtgac gcggccggtc
      901 gtgaggctct ccgactcgac ccggacgccg acctccatcg aggtcgtgaa cgcttcgttc
      961 accgaggcct tgagcgtgac caggtcgccg aggtacaccg gctggaggaa cgacatctcg
     1021 tccatgaccg ccgtgaccgc gagaccgccg gcgtgcttcg cggcggcgag gcccgcggcg
     1081 ttgtcgacga gcttcatgat cgtgccgccg tgcacgttgc cggcgatgtt cgcgtcggcg
     1141 acctgcatga tctgggcgag gacgatgcgg gacgccgaga cgggcttggg ggtgaggtcg
     1201 accggcccgg cggagccctg gatcacccgg ccgccccgat cgagcggagg acctgccgcg
     1261 cgatcacgat ccgctggacc tggttcgtcc cctcgtagat ctgggtgatc ttcgcgtcgc
     1321 gcatcatccg ctcgacgccg tactcgcgca tgtacccgta gccgccgagc atctggaccg
     1381 cgtcggtggt gaccgccatc gcggtgtcgg aagcgtggca cttcgcgacc gcggcgtggt
     1441 aggtcatctt cgggtccttc gcgtcggtcg cggccgcggc gcggtaggtg agcatccgcg
     1501 cggcctcgac ctgcatcgcc atgtcggcga gcatgaactg cagaccctgg aactccgcga
     1561 tcttcttccc gaactgctcg cgctcggtga ggtagcgcga ggcgacgtcg aaggctccct
     1621 gcgcgatgcc gagcgcctgc gccgcgatcg tcggccgcga gtagtcgagg gtctgcatcg
     1681 cgtacgtgaa gcccttcccc tcctcgccga gcagacggtc ctcggggatc tcgacgttgt
     1741 cgaggtagac ctcccgcgtg ggcgacccgc ggatgccgag cttcttctcg gacttgccga
     1801 ccgagaagcc ttcgtcatcg gagtggacga tgaacgcgga gatgcccttc gtgcgcatcg
     1861 acgggtcctt cgtcgcgaac accgtgtagc ggtcggaggc cccggccccg gtgatgaagc
     1921 gcttcgtccc gtgcagccgg aagccgccgt cggtccgctc gtaacgcgtc tggagcgacc
     1981 cggcgtcgga ccccgcgccc ggctccgtga gcgcgtacga catctggttg tccccacgcg
     2041 agatcccgcc gaacagcgcg gtcttctgct cgtcggagcc cgccagcagg acggggatcg
     2101 ccccgagccg gttgacgagc gggatgagcg acgcgctcgc cgagatccgc gcgatctcct
     2161 cgaccatgac ggccatcgtc agcgagccgc caccggcccc gccgtgctcc tccgggtacc
     2221 cgaccgccat cagctcgttc tcgacgaaga gcttgtggac gtcccacggg aactcgtcct
     2281 cctcgtcgat gtccgccgcc cgcgacgcga gcctctcctc acagatctcg cgcacgagcc
     2341 cccggaactc ctcgagctcc tccggcagat tgaagaggtc catgcccgga atcttagggc
     2401 atggtcaccc actaggctca ggctcggtct cgcctcggcg gcctcaaggg ccctccaact
     2461 ccgccgcacg gagatctcta tgtcgtcgct cggtcactgc tctgctgttt gcgccagccg
     2521 tagcgggtga ggccttgcgg cttgtggatg ttgagcatga ggacgaccaa caggacgatg
     2581 gctcccccca ccgagtgcgg caaggtgtgg cccaggcccc gtac
//
LOCUS       DATLGG010000041        13417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_191464, whole genome shotgun sequence.
ACCESSION   DATLGG010000041 DATLGG010000000
VERSION     DATLGG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191464"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VM638_01035"
     CDS             complement(<1..614)
                     /locus_tag="VM638_01035"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HVM11046.1"
                     /translation="MEGARVGMDLADSVGVEEAASFVADLLERPASQIRVAEGQLGLA
                     EDLVVEGHPDAFALERVPVSDPGTVWAVDGGSCTVADGRSFQVAATRAARVRLHGGVT
                     DLVESPPLHVRALSAAEMQTLAREVLAELCGTAPERIPDLPRPVDALREWAEWGEVAR
                     TVREAARGDLVLVDGSLHGGPLVPPEVVRRVHAEAVERGVALAGV"
     gene            912..1238
                     /locus_tag="VM638_01040"
     CDS             912..1238
                     /locus_tag="VM638_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HVM11047.1"
                     /translation="MTLEEPDIIEDEETEEEEDGADIGETPEDVESSLDELLAKREKT
                     ESEASPDATIEEAIPPSSREERVETLTVKVVPQQPTEFTCRNCFLVKHRSQLANKKRM
                     LCRDCA"
     gene            1286..3490
                     /gene="lnt"
                     /locus_tag="VM638_01045"
     CDS             1286..3490
                     /gene="lnt"
                     /locus_tag="VM638_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVM11048.1"
                     /translation="MALAAAYPPIDAGPLALVALIPLLAAATRAEPRPAALAGLLFGL
                     VHFGLVTSWLTTQTWLGWGIFTLWQALMAAVALGLISMAWRDARPIRSAFAAAALWAT
                     VEWIRGAWPLGGVSWMDLGTSQHDNPLLVPAASWIGSLGLSFVVVAINALLLVAVRRG
                     APRILPALLAVGLALAPALIPLGAPTGGEVDVAIVQGNVPERLAEEDRLIRDRLVVEN
                     HARVSERLVEDPPDLMVWPENALDADPTRDPLLGSRVFDVVTSVGSHAVIGAITTDAE
                     GQRFNENLLYAPNGALVDRYAKNHILPFGEFVPLRRYLSWVPDVARVRDDLTPGEEPG
                     RFAIPGASFATAICFENNFAALIRSFTTERDGFIVLTTNNSSFMRSAAPDQHVALSEM
                     RAVETGRWVVHAALTGRSAFIDHRGRVLESSDLFVPAMLRRAVPQANGRTVYDRIGGA
                     IPWVLLASSVLALVAVRPARRRRPQPLAPDPRVAVVLPTYNEAATVGEAIRRVRAQGV
                     SVIVVDDSSPDGTASVVRDLGDPEVELIERSGKLGLASAYATGFRRALEAGFDLVVEM
                     DADLSHRPEDLPGLLAAAGDHDLVIGSRYIPGGGVRNWGPFRRVLSRAGNVYARLILG
                     LPVRDATSGFRVFRRGLLARLLEDGIRSEGYAFQVELAYRAWRLGYAVGEAPILFEER
                     RAGESKISQAIVVEALWEILRWGVRDRLRRVSGARSARSRGATADGSRAPGG"
     gene            complement(3423..4157)
                     /locus_tag="VM638_01050"
     CDS             complement(3423..4157)
                     /locus_tag="VM638_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HVM11049.1"
                     /translation="MPTYPDPAGSTPFEWTGRGGPFTLLLSPSVFPPSKTSAVVADAL
                     EVRPGETVIDVGCGSGVLSFVAAKLGAGRVYGSDISAPSVEVARANAERLGLADRTEF
                     RAGSLFEPLRDVEAEVVIGDVSGIPDEVAEVSGWFPGGYAGGATGAEVPIQMLETIGT
                     CLKSGGRLYLPTASLQDENRVLAAARRIFGERNIVPLAEREFPLPALVARSKALAKLM
                     ADGLLTLRERGSRLLWRLAIWRCERR"
     gene            4194..5339
                     /locus_tag="VM638_01055"
     CDS             4194..5339
                     /locus_tag="VM638_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HVM11050.1"
                     /translation="MDEDALTLGIEEEFQIVGPDGELRAHIEAVMERAFAISDLGDLV
                     KPEFLQSTVEVGTKVCRDVAEARVEITRMRRGLATILDEAGLRLVSAGTHPFSRWQDQ
                     QVTEKERYHTLEQEMQDVARELLIFGMHVHIGIPDPELRIDIFNEARYFLPHLLALSA
                     SSPFWMGRDTGLKSYRSVVWSRFPRTGIPPHFESWAEWQNYVQLLQKTNSLPDPSKIW
                     WDLRPHGVFPTIEFRICDAATRIDETICLAAIIQAFVAKLIKLRQQNLGFRTYAPKLI
                     RENKWRAMRMGIEGKLIDFGKQEEVPEPDLIEEIIGFVDDVVDDLGSRKEVEYARTIL
                     REGTSAERQLRIFRETGDLQKVVDHLAEETVAGTEPEVVAEAPALED"
     gene            5449..5763
                     /locus_tag="VM638_01060"
     CDS             5449..5763
                     /locus_tag="VM638_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11051.1"
                     /translation="MPLPTRILVGLAVAGAAFALLAPLRCRPAIEVLETGPGITVDRP
                     TAGEVTCDRLLLPDHRGPVEPARWPAALGAVVLGGVTASALPASAREAGPPRPGETEE
                     DR"
     gene            5760..6755
                     /locus_tag="VM638_01065"
     CDS             5760..6755
                     /locus_tag="VM638_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003954882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVM11052.1"
                     /translation="MIRAAGLVKRYGEVTALAGLDLEATPGSVLGLLGPNGAGKTTAV
                     RILTTLLEADGGEATVAGLDLRTQPGRVRERIGLSGQHAAVDEHLTGFENLDMVGRLY
                     RLGARRSRERARELLERFDLAEAADRPAKTYSGGMRRRLDLAAALVAEPEVLFLDEPT
                     TGLDPRSRAQMWDTIAELVRGGSTVLLTTQYMEEADRLADHIVVIDRGRKIAEGTADE
                     LKSQVGGERIELVVEDASAIPEVMTVLAEVSDEEVLVDEQARRLTAAAGQGDGAGDLR
                     RVLEMLGDRRIGVVDVGLRRPTLDDVFLTLTGHSAEHVVEEGESPGGGTDDGGAS"
     gene            6752..7552
                     /locus_tag="VM638_01070"
     CDS             6752..7552
                     /locus_tag="VM638_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM11053.1"
                     /translation="MSTRFAPIADGLVVAKRNLIKIRRVPELLIWTTMSPIMFVLLFA
                     YVFGSSIEIPGTTYREFLIAGVFVQTVIFGSTYTGAGLAEDMTKGIIDRFRSLPMSDA
                     AVLVGRTTSDILYNSISITVMSITGLIVGWRIRTAFLPALGGFLLLLLFAYAASWIMA
                     YVGLRVASVEVVQNASFMFIFPLTFIANTFVRAELLPTPLRVFAEWNPVSAVAQAVRE
                     AFGNTPAGPPPDVWSLRNPVLYSLIWVAIILAVFIPLSVGRFKKAATR"
     gene            complement(7554..9032)
                     /gene="gcvPB"
                     /locus_tag="VM638_01075"
     CDS             complement(7554..9032)
                     /gene="gcvPB"
                     /locus_tag="VM638_01075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HVM11054.1"
                     /translation="MSRTGAPVRVGDPEIPVTLKELSRPGRRGSSFPALDVPAAEIPE
                     EHRRVDAAPLPEVGERDLVRHYTRLSQRNYGVDTGFYPLGSCSMKYNPKIADTVSALP
                     GFQRLHPHQPDHSAQGALELLWRLERALCEVTGMTRATFQPAAGASGELTGLLIMRAY
                     HQRNGERRRKIVIPDSAHGTNPASVTLGGYDVVEVPSDARGLVDLSALEKLVDDDVAG
                     LMITNPNTLGLFEQDITEIARIIHEAGGLLYYDGANLNAIVGRVRPGDMGFDIVHMNT
                     HKTFATPHGGGGPGAGPVAVKGRLAPFLPAPVVQRADTGRFYLDQDRPHSIGRIQGFN
                     GNFGVLVRAYAYVFGHGGDGLREVGERAVLNANYLAERVKEAFPLAFEGRPMHEFVST
                     ARGLRKHGIRAMDVAKRLIDLGYYPSTVYFPLIVEEAMMVEPTETESRETLDAFADAL
                     LQVAREAADEEGQQLLHEAPVTTPVRRLDEAKAARTPRCRWD"
     gene            complement(9029..10333)
                     /gene="gcvPA"
                     /locus_tag="VM638_01080"
     CDS             complement(9029..10333)
                     /gene="gcvPA"
                     /locus_tag="VM638_01080"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HVM11055.1"
                     /translation="MRFAPHTDDDVREMLDVLGLRSLDDLYAHIPAEVQLDRPLDLPP
                     GVSEMELLADLRSLAAKNRHMDEVVCFAGGGAYDHYVPALVWSLAGRSELQTSYTPYQ
                     PELSQGVLQALFEYQSMICDLTGMEVSNASLYDGATALVEAVNMARGATGRSRVLVAG
                     RLDPRYLETLRTYGRGPGYEPEIVAATGPTGKGAWDDVACVIVQHPDAHGVLGPARQA
                     FEAARAAGARAIHVFDPLALGILASPRELGADIAVAEGQVLGNHLSYGGPYLGIIAAT
                     QQDVRRMPGRIVGETSDVDGNLGYVLTLQTREQHIRRAKATSNICTNQTLMAIAAAVY
                     LSWLGPRGLVEVGEACAAKARYAADRLVAAGARLAHPDEPFFKEFALTFDRPATEVRD
                     TLLEVGYLVGPVVDDSLLVACTERRTRGEIDGLAEALGEVLA"
     gene            complement(10408..10782)
                     /locus_tag="VM638_01085"
     CDS             complement(10408..10782)
                     /locus_tag="VM638_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11056.1"
                     /translation="MRAAPAETPPDRPPSDSVDFAHESERQFARLLDFYQIEWEYEPR
                     SFDIEWDEEGNVTARFTPDFYLPAWDLYIEITTMNQKLVTKKNRKVRRLKELYPEVSC
                     KIFYQRDYLSLVTKYGLEDEAG"
     gene            complement(10779..11240)
                     /locus_tag="VM638_01090"
     CDS             complement(10779..11240)
                     /locus_tag="VM638_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVM11057.1"
                     /translation="MADEQQGYRAPQVGEIIGISYRQLDYWDRTGLVKPSIKSAEGSG
                     TQRLYSFQDLLQLKVIKQLLDAGVSLQKVRKAVEYLQGHLREQPQGLNLLSDGDRIYA
                     AASPGEVFDVLQKGQGVFAIAVDRVWSDLEGSVKKAAKAGRSPRAAGAGRA"
     gene            complement(11300..11773)
                     /locus_tag="VM638_01095"
     CDS             complement(11300..11773)
                     /locus_tag="VM638_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HVM11058.1"
                     /translation="MIEMRLEGVRVELPANQPIVLLKERTGERYLPIWIGTAEATAIA
                     LAIQGVTPARPMTHDLMKNMLDELQVVVERILITDLREGTYYATIQMARDGTRLEISS
                     RPSDAIALAARIEVPIFAHEDVMAEAGITMSEDEEQEVEKFREFLDRVSPEDFEE"
     gene            complement(11816..12397)
                     /gene="hpt"
                     /locus_tag="VM638_01100"
     CDS             complement(11816..12397)
                     /gene="hpt"
                     /locus_tag="VM638_01100"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HVM11059.1"
                     /translation="MDPGVELLYGAREIRDRVRALGARITEEYAGREPILVGVLKGAA
                     TFLADLARVVGTNISVDFISISSYGGGTPSGVVRIMKDLDRDIGGRDVIVVEDIVDTG
                     LTMAYLLSVLAARNPASVEVCTLLDKSVRRITPLEIRYRGFDCPDRFVVGYGMDVGER
                     FRNLPFIVAVADPTALADDPAPLLPYLDGEPSS"
     gene            complement(12402..>13417)
                     /locus_tag="VM638_01105"
     CDS             complement(12402..>13417)
                     /locus_tag="VM638_01105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVM11060.1"
                     /translation="LFERRGGRRLPIASTTKVMTALIVLESTTPFEVVVVSERAAAEP
                     GARLGLRAGERIEVRQLLQALMLQSANDAAVALAEHVSGSVERFVRRMNRRARALGLR
                     RTRFASPNGLDDRGRSTARDLARLTVEAMRHERFAEIVATKRHRVPADAGPDRRVQNR
                     NVLLWLYRGATGVKTGYTAAAGYCLVATAERRDLHLVAVVLGSPREAFSDTAALLNHG
                     FRAYERRTLSPAGGSLPAAGIAGRRVPLVLAEEASAVLRRGEEVEREVRLEPGLRLPV
                     REGQEVGTVTYTAGGAVVAEVPVRAAAAVEPAPVVPPAWWRRALDAVRAFFTAWAAEA
                     PVR"
BASE COUNT         1898 a         4837 c         4735 g         1947 t
ORIGIN      
        1 acccctgcga gcgccacccc gcgctcgacc gcttcggcgt ggacccggcg cacgacctcc
       61 ggcggaacga gcgggccgcc gtggagggaa ccgtcgacga gcacgaggtc gcccctcgcc
      121 gcttcgcgca cggttcgcgc cacctcgccc cactccgccc actcgcggag ggcgtccacc
      181 gggcgaggca ggtcggggat ccgctcgggc gccgtgccgc agagctcagc gagcacctcg
      241 cgagcgaggg tctgcatctc ggccgccgag agcgcccgca cgtgcagggg aggggattcg
      301 acgagatcgg tcacgccgcc gtggaggcgc acgcgagccg cccgcgtggc cgcgacctgg
      361 aacgaccgcc cgtcggcgac ggtgcaggag cccccgtcga ccgcccagac ggtgccggga
      421 tcgctcacgg ggacccgctc aagcgcgaag gcgtccggat gaccctcgac gacgaggtcc
      481 tcggccaggc cgagctgccc ctcggcgacc cggatctgcg acgccggtcg ctcgaggagg
      541 tcggcgacga agctcgccgc ctcctcgacc ccgacgctgt ccgccaggtc catgcccacg
      601 cgggccccct ccatccaccg caggatacgc ggcgccgccg acacgccgcg cagcacgtcg
      661 agcgggcccg gttgacacct ctaccgggcg gccgtagcat gcccctcgtc cgccggctcg
      721 accggcgcga cgaaggaccc acagaggctg agtagagaac gcggcgcctc ggcgccgcgg
      781 ccccaaaggt ttctgtgggt ccttcgtcgt tcccgcgttt cccgccgccg gccgggggta
      841 gacaggcagc gccgacgccg cctcccggcg tttcagcgga acgggactac aatgcgcagc
      901 cgaggacgac catgacgctc gaagaaccag acatcatcga ggacgaagag accgaagagg
      961 aggaggacgg cgccgacatc ggggagaccc ccgaggacgt cgagtcgagc ctcgacgagc
     1021 tgctggcgaa gcgggagaag accgagtccg aggcctctcc cgacgccacg atcgaggagg
     1081 cgatcccgcc gagcagccgc gaggagcggg tcgagaccct gacggtcaag gtggttcccc
     1141 agcagccgac cgagttcacg tgccggaact gcttcctggt caagcatcgc agccagctcg
     1201 cgaacaagaa gcgcatgctc tgccgcgact gcgcgtagcc gacggcacgc gagggtccct
     1261 cctccttccc agcttcgcct ccggagtggc gctcgccgcc gcgtacccgc cgatcgacgc
     1321 ggggccgctc gcgctcgtcg cgttgatccc gctcctcgcc gccgcgacgc gcgccgagcc
     1381 gcgacccgcc gcgctggccg gactcctctt cgggctcgtc cacttcgggc tcgtcacgtc
     1441 gtggctgacg acgcagacct ggctcggctg gggcatcttc acgctctggc aggcgctcat
     1501 ggcagccgtg gcgctcggcc tgatctcgat ggcctggcgc gacgcgcgcc cgatccgttc
     1561 cgccttcgcc gcggcggcgc tgtgggcgac ggtcgagtgg atccgggggg cgtggccgct
     1621 cgggggagtg tcctggatgg acctcggcac gagccagcac gacaacccgc tcctggtccc
     1681 ggcggcgtcg tggatcggct cgctcggtct ttccttcgtc gtcgtcgcga tcaacgcgct
     1741 cctgctcgtc gccgtccggc gcggggcccc gcgcatcctt cccgcgctgc tcgccgtcgg
     1801 tctggcgctg gccccggcgc tgatcccgct gggggccccg accggaggcg aggtggatgt
     1861 cgcgatcgtc caggggaacg tgccggagcg ccttgccgag gaggaccggc tgatccgcga
     1921 ccgcctcgtc gtcgagaacc acgcccgcgt gagcgagcga ctcgtcgagg atccgcccga
     1981 cctcatggtc tggccggaga acgccctcga tgccgacccg acgcgcgatc cgctgctcgg
     2041 cagccgcgtc ttcgacgtcg tcacctccgt cggctcgcac gccgtgatcg gtgcgatcac
     2101 gaccgacgcc gagggacagc gcttcaacga gaacctcctg tacgcgccga acggggcgct
     2161 cgtcgaccgg tacgcgaaga accacatcct cccgttcggt gagttcgtcc cgctgcgccg
     2221 gtacctgtcg tgggtccccg acgtcgcccg cgtccgcgac gacctcaccc cgggggagga
     2281 gcccgggcgc ttcgcgatcc ccggcgcctc cttcgccacg gcgatctgct tcgagaacaa
     2341 cttcgcggcg ctgatccgtt cgttcaccac cgagcgtgac ggcttcatcg tcctgacgac
     2401 gaacaactcg agcttcatgc gcagcgcggc gcccgaccag cacgtcgcgc tgtcggagat
     2461 gcgcgcggtc gagacggggc gctgggtcgt ccacgcggcg ctcaccggtc ggagcgcgtt
     2521 catcgaccac cgcgggcggg tgctcgaaag cagcgacctg ttcgtgccgg cgatgctgcg
     2581 acgagccgtc ccgcaggcga acggccggac ggtctacgac cgcatcggcg gcgcgatccc
     2641 gtgggtgctc ctcgcatcgt ccgtcctcgc cctcgttgcg gtccggcccg cgcgacgacg
     2701 ccggccgcag ccgctcgcac cggacccccg ggtggccgtg gtcctcccca cgtacaacga
     2761 ggcggccacc gtgggcgagg cgatccggcg cgtccgcgcg cagggcgtct ccgtcatcgt
     2821 cgtcgacgat tcctcaccgg acgggacggc ctcggtggtc cgggacctcg gcgaccccga
     2881 ggtcgagctg atcgagcgga gcgggaagct cggcctcgcc tccgcctacg ccacggggtt
     2941 ccgccgggcc ctcgaggccg ggttcgacct cgtcgtggag atggatgcgg acctctcgca
     3001 ccgtccggag gacctcccgg ggctcctcgc ggcggcgggg gaccacgacc tcgtgatcgg
     3061 aagccgctat atccctggcg ggggggtccg caactggggc ccgttccggc gggtcctgtc
     3121 gcgggcgggg aacgtctacg cgcgcctgat cctcggcctg ccggtccgcg acgcgacgag
     3181 cggcttccgc gtcttccgcc ggggactgct cgcgcggctc ctcgaggacg gcatccggag
     3241 cgagggctac gcgttccagg tcgagctcgc gtaccgggcg tggcgactcg ggtacgcggt
     3301 gggggaggct ccgatcctgt tcgaggagcg gcgggcgggg gagtcgaaga tctcgcaggc
     3361 gatcgtcgtc gaggcgctgt gggagatcct ccgctggggg gtccgggacc gcctccggcg
     3421 cgtcagcggc gctcgcagcg ccagatcgcg aggcgccaca gcagacggct cccgcgctcc
     3481 cggagggtga gcagcccgtc cgccatcagc ttcgcgagag ccttcgaacg ggcaacgagc
     3541 gccggcagcg ggaactcgcg ctcggccagc gggacgatgt tccgctcgcc gaagatccgc
     3601 cgggccgccg ccagcacgcg gttctcgtcc tggagggacg ccgtcggcag gtagagccgc
     3661 ccgccggact tcaggcaggt cccgatcgtc tcgagcatct ggatcggcac ctcggctccg
     3721 gtggcgcccc ccgcgtaccc ccccgggaac cacccgctga cctccgccac ctcgtcgggg
     3781 atcccggaca cgtcgccgat cacgacctcg gcctcgacgt cgcggagcgg ctcgaagagc
     3841 gaccccgctc ggaactcggt ccggtccgcc aggccgagcc gctcggcgtt cgcccgcgcg
     3901 acctcgaccg agggcgcgga gatgtcggag ccgtagacgc ggccggctcc gagcttcgcc
     3961 gcgacgaagc tcagcacgcc gctcccgcat ccgacgtcga tgacggtctc gcccgggcgg
     4021 acctccaggg cgtcggcgac gaccgcgctc gtcttcgagg gcgggaacac gctgggggag
     4081 agcaggaggg tgaagggtcc tccccggccc gtccactcga acggggtgga gccggcggga
     4141 tctgggtagg tgggcatctg cacagcctac cccagcagtg gttgaatcgc tccatggacg
     4201 aagacgccct gacgctcggc atcgaggagg agttccagat cgtcgggccc gacggcgagc
     4261 tccgggccca catcgaggcg gtgatggagc gcgccttcgc gatctccgac ctcggggacc
     4321 tcgtgaagcc cgagttcctc cagtcgacgg tcgaggtcgg gacgaaggtc tgccgcgacg
     4381 tcgccgaggc gcgggtggag atcacccgga tgcgccgcgg gctcgccacg atcctcgacg
     4441 aggccggcct tcgtctcgtc tcggccggca cccatccgtt ctcgcgctgg caggaccagc
     4501 aggtgaccga gaaggagcgc taccacacgc tcgagcagga gatgcaggac gtcgcgcggg
     4561 agctcctcat cttcgggatg cacgtgcaca tcggcatccc cgacccagag ctccggatcg
     4621 acatcttcaa cgaggcgcgg tacttcctcc cgcacctgct cgcgctctcg gcgtcgagcc
     4681 ccttctggat gggccgcgac accggcctca agtcctaccg cagcgtcgtg tggtcccggt
     4741 tcccgcgcac cgggatcccg ccgcatttcg agtcctgggc cgagtggcag aactacgtcc
     4801 agctcctgca gaagacgaac tctctgccgg atccgtcgaa gatctggtgg gaccttcgtc
     4861 cccacggcgt cttcccgacg atcgagttcc ggatctgcga cgccgcgaca cggatcgacg
     4921 agacgatctg cctcgccgcg atcatccagg ccttcgtcgc caagctgatc aagctgcgac
     4981 agcagaacct cggcttccgg acgtacgcgc cgaagctgat ccgcgagaac aagtggcgcg
     5041 cgatgcggat gggcatcgag ggcaagctca tcgacttcgg caagcaggag gaggtcccgg
     5101 agcccgacct catcgaggag atcatcggct tcgtcgacga cgtcgtggat gacctcggct
     5161 cgcgcaagga ggtcgagtac gcccggacga tccttcgtga agggaccagt gccgagcgtc
     5221 agctccggat cttccgggag acgggtgacc tccagaaggt cgtcgaccac ctcgccgagg
     5281 agacggtcgc gggcaccgag cccgaggtcg tcgcggaggc cccggccctc gaggactgac
     5341 cccgcgaggg ccccttccct cggacgccca taatggacct tatgtaaagt agagggagga
     5401 ggagtccctc ccgtgcgttt ccgctgcagg cgtgtaggct cccccgcgat gccgctgcca
     5461 acccggatcc tggtcggact cgccgtggcc ggtgctgcct tcgccctcct ggcgccgctc
     5521 cggtgccggc ccgcgatcga ggtgctcgag acgggccccg ggatcaccgt ggaccggccg
     5581 acggccggcg aggtcacctg cgaccgcctc ctcctgccgg accaccgcgg ccccgtcgaa
     5641 ccggctcgct ggcccgcggc gctcggggcc gtcgtcctcg gcggggtgac ggcctccgcg
     5701 ctgccggcgt ccgcgcgtga ggccggccct ccccggccgg gggagacgga ggaggatcga
     5761 tgatccgcgc ggcgggcctg gtgaagcgct acggcgaggt caccgccctc gcggggctgg
     5821 acctcgaggc gaccccgggg agcgtcctcg ggttgctcgg tcccaacggg gccgggaaaa
     5881 cgaccgcggt acggatcctg accacgctcc tggaggccga cggcggcgag gcgaccgtgg
     5941 ccgggctcga cctccgcacg cagccagggc gcgtccgcga gcggatcggc ctgtccggcc
     6001 aacacgccgc ggtggacgag cacctgaccg gcttcgagaa cctcgacatg gtcggccgcc
     6061 tctaccggct cggggcccga cgctcgcggg agcgcgcgcg ggaactgctc gagcgcttcg
     6121 acctcgccga ggcggcggat cggccagcga agacctactc cggagggatg cgccggcgcc
     6181 tcgacctcgc ggccgccctc gtcgccgagc cggaggtgct tttcctcgac gagccgacca
     6241 ccgggctcga tccacgcagc cgcgcacaga tgtgggacac gatcgcggag ctcgtccgcg
     6301 gcggttcgac cgtcctgctg accacgcagt acatggagga ggcggatcgg ctcgccgacc
     6361 acatcgtcgt catcgaccgc ggccgcaaga tcgccgaggg gacggctgac gagctcaagt
     6421 cgcaggtcgg cggcgagcgg atcgagctcg tggtcgagga cgcctccgcg atcccggagg
     6481 tgatgacggt gctcgccgag gtgagcgacg aggaggtgct cgtggacgag caggcacgac
     6541 ggctcacggc ggccgccggg cagggcgatg gggccggcga cctgcggcga gtcctcgaga
     6601 tgctgggcga tcgccggatc ggcgtcgtgg acgtcggcct tcgacgcccc acgctcgacg
     6661 acgtcttcct caccctgacc ggccactccg cagagcacgt ggtcgaggag ggcgagtcgc
     6721 ccggcggggg cacggacgac ggaggcgcct cgtgagcacg cggttcgcac cgatcgcgga
     6781 tgggctcgtc gtcgcgaagc ggaacctgat caagatccgg cgcgtccccg agctgctgat
     6841 ctggacgacg atgtcgccga tcatgttcgt cctgctgttc gcctacgtct tcggcagctc
     6901 gatcgagatc cccggcacga cctaccgcga gttcctgatc gccggggtgt tcgtccaaac
     6961 cgtgatcttc ggctcgacct acaccggggc cggtctcgcc gaggacatga cgaaggggat
     7021 catcgacagg ttccgttccc tcccgatgtc ggatgcggcg gttctcgtgg gacgcacgac
     7081 gagcgacatc ctctacaact cgatctcgat cacggtgatg tcgatcaccg gcctcatcgt
     7141 cgggtggcgg atccgaaccg cgttcctgcc ggcgctcggc gggttcctgc tgctgctcct
     7201 gttcgcgtac gcggcctcgt ggatcatggc ctacgtcggc ctgcgcgtgg cgagcgtgga
     7261 ggtggtccag aacgcctcgt tcatgttcat cttcccgctg accttcatcg cgaacacgtt
     7321 cgtccgcgcg gagctgctgc ccactcccct gcgcgtcttc gccgaatgga acccggtctc
     7381 cgcggtggcc caagcggtgc gcgaagcctt cggcaacacc ccggccggtc ccccacccga
     7441 cgtctggtcc ctgcgcaacc cggtcctcta ctcgctgatc tgggtcgcga tcatcctcgc
     7501 ggtgttcatc cccctctcgg tcggccgctt caagaaggcc gcgacccgct gagtcagtcc
     7561 cagcggcaac ggggggtccg agcggccttc gcctcgtcga gtcgccggac cggggtcgtc
     7621 accggagcct cgtggaggag ctgctggccc tcctcgtccg cagcctcgcg cgcgacctgg
     7681 aggagcgcgt cggcgaacgc gtccagcgtc tcccgggact cggtctcggt cggctcgacc
     7741 atcatcgcct cctcgacgat cagcgggaag tagaccgtcg aggggtagta gccgaggtcg
     7801 atgaggcgct tcgccacgtc catcgcgcgg atgccgtgct tgcgcaggcc cctcgcggtc
     7861 gagacgaact cgtgcatcgg ccgcccctcg aaggcgagcg ggaacgcctc cttcacccgt
     7921 tcggcgaggt agttcgcgtt cagcaccgcg cgctccccca cctcacgcag gccgtcgccg
     7981 ccgtggccga agacgtacgc gtaggcgcgg acgagcacgc cgaaattccc gttgaagccc
     8041 tggatccgcc cgatcgagtg cgggcggtcc tggtcgaggt agaagcgccc ggtgtccgcc
     8101 cgctggacga ccggcgccgg caggaacggc gcgagccgcc ccttcaccgc gaccggcccc
     8161 gcgccgggtc ccccgccgcc gtggggcgtc gcgaacgtct tgtgcgtgtt catgtgcacg
     8221 atgtcgaagc ccatgtcgcc ggggcgcacg cgaccgacga tcgcgttgag gttcgcgccg
     8281 tcgtagtaca ggagcccccc tgcctcatgg atgatccgcg cgatctcggt gatgtcctgc
     8341 tcgaacaggc cgagcgtgtt cgggttcgtg atcatgagcc cggcgacgtc gtcgtcgacg
     8401 agcttctcga gggccgagag gtcgacgagc ccgcgcgcgt ccgacggcac ctcgacgacg
     8461 tcgtacccgc cgagcgtgac cgacgccggg ttcgtgccat gcgccgagtc ggggatcacg
     8521 atcttgcgcc gccgctcgcc gttccgctgg tggtaggccc gcatgatcag gaggcccgtg
     8581 agctcgcccg aggcgccggc ggccggctgg aaggtcgccc gcgtcatccc cgtcacctcg
     8641 cagagcgccc gctcgagccg ccagaggagc tcgagggcgc cctgggcgct gtggtcgggc
     8701 tgatgcgggt ggagccgctg gaagccggga agcgcggaga ccgtgtccgc gatcttgggg
     8761 ttgtacttca tcgagcagga ccccagcgga tagaagccgg tgtccacgcc gtagttccgc
     8821 tgggacagtc gcgtgtagtg gcgaacgagg tcccgctccc ccacctccgg cagcggagcc
     8881 gcgtccacgc gccgatgctc ctccgggatc tcggccgcgg gaacgtcgag ggccgggaac
     8941 gaggagccgc gccgtcccgg ccgggagagc tccttcagcg tcacggggat ctcgggatcg
     9001 ccgacgcgga ccggggcgcc ggtgcggctc acgcgagcac ctcccccagg gcctcggcga
     9061 ggccgtcgat ctccccgcgc gtccgtcgct ccgtgcaggc gacgagcagt gagtcgtcga
     9121 ccacggggcc gaccaggtag ccgacctcga gcagcgtgtc acgcacctcg gtcgccgggc
     9181 ggtcgaacgt cagcgcgaac tccttgaaga acggctcgtc gggatgcgcc agccgcgcgc
     9241 ccgccgcgac cagccggtcg gccgcgtagc gcgccttcgc cgcgcaggcc tcacccacct
     9301 cgacgagacc ccgcgggccg agccaggaca ggtagaccgc cgcggcgatc gccatgagcg
     9361 tttggttcgt gcagatgttc gaggtcgcct tcgcccgccg gatgtgctgc tcgcgcgtct
     9421 gcagggtcag cacgtatccg aggttgccgt cgacgtcgct cgtctcgccg acgatccgcc
     9481 ccggcatccg ccggacgtcc tgctgggtcg cggcgatgat cccgaggtac ggacccccgt
     9541 agctcaggtg gttgccgagg acctgcccct cggcgaccgc gatgtcggcg ccgagctccc
     9601 gcggcgaggc gaggatcccg agggcgaggg ggtcgaagac gtggatcgcc cgcgcgccgg
     9661 cggcccgcgc cgcctcgaac gcctgccggg ccgggccgag gacgccgtgc gcgtcggggt
     9721 gctggacgat cacgcaggca acgtcgtccc acgcgccctt ccccgtcggc cccgtggcgg
     9781 cgacgatctc cggctcgtag cccggacccc ggccgtaggt gcgcagcgtc tcgaggtagc
     9841 gcgggtcgag ccgtccggcc acgaggacgc gcgagcgccc ggtcgcgccg cgggccatgt
     9901 tcacggcctc cacgagcgcg gtcgcgccgt cgtagaggct cgcgttcgac acctccatcc
     9961 cggtgaggtc gcagatcatc gattggtact cgaagagcgc ctggagcacg ccctgcgaca
    10021 gctccggctg gtacggcgtg taggaggtct ggagctcgga gcggccggcg agcgaccaca
    10081 cgagggcggg gacgtagtgg tcgtaggccc cgccgccggc gaagcagacc acctcgtcca
    10141 tgtgccggtt cttcgccgcg agcgagcgga ggtccgcgag gagctccatc tcggagaccc
    10201 ccggcgggag gtcgagcggg cggtcgagct ggacctcggc ggggatgtgg gcgtagaggt
    10261 cgtcgagcga gcgcagcccg agcacgtcga gcatctcgcg gacgtcgtcg tcggtatggg
    10321 gggcgaatcg cacgttgcct gggacctcga tccgggggga aggcggagcg cattctaccc
    10381 gatgctccgc cgaacgggtc ccctccccta ccccgcttcg tcctccaggc cgtacttcgt
    10441 cacgagcgag aggtaatcgc gctggtagaa gatcttgcag ctgacctcgg gatacagctc
    10501 cttcaggcgc cggaccttgc ggttcttctt cgtcacgagc ttctggttca tcgtcgtgat
    10561 ctcgatgtag aggtcccacg ccgggaggta gaagtccggg gtgaaacggg cggtgacgtt
    10621 cccctcctca tcccactcga tgtcgaacga gcgcggctcg tactcccact cgatctggta
    10681 gaagtcgagc aggcgggcga actgacgttc gctctcgtgg gcgaaatcga cgctgtcgct
    10741 gggcgggcgg tcgggcgggg tctcggccgg ggccgccctc aagcccttcc cgcgccggcg
    10801 gctcgcggcg agcgtccggc cttcgcggcc ttcttcacgg agccttcgag gtcgctccag
    10861 acgcggtcca cggcgatcgc gaagaccccc tggcccttct ggaggacgtc gaagacctcg
    10921 cccggtgagg cggcggcgta gatgcggtcg ccgtccgaca ggaggttgag cccctgcggc
    10981 tgctcgcgga ggtgtccctg gaggtactcg accgccttgc ggaccttctg gagcgagacg
    11041 ccggcgtcga ggagctgctt gatgaccttg agctgcagca ggtcctggaa gctgtacaga
    11101 cgctgcgtcc cggacccctc ggcgctcttg atgctcggct tcaccaggcc cgtgcggtcc
    11161 cagtagtcga gctggcggta ggagatgccg atgatctccc cgacctgcgg tgcgcggtat
    11221 ccctgctgct cgtctgccat gacaacctcg taattacgtg ggcgtcggca accgtacccc
    11281 cgggaggggc gtgagtcaac tactcctcga aatcctccgg gctgacccga tcgaggaact
    11341 cgcggaactt ctcgacctcc tgctcctcgt cctcgctcat cgtgatgccg gcctcggcca
    11401 tcacgtcctc gtgggcgaag atcgggacct cgatccgcgc cgcgagcgcg atcgcgtcgg
    11461 acgggcggga ggagatctcc aggcgtgtgc cgtcccgggc catctggatc gtcgcgtagt
    11521 acgtgccctc gcgcaggtcc gtgatgagga tgcgctcgac gaccacctgg agctcgtcga
    11581 gcatgttctt catgaggtcg tgcgtcatcg gccgggccgg cgtcacgccc tggatcgcga
    11641 gcgcgatcgc cgtagcctcg gcggtcccga tccagatcgg gaggtagcgc tcgccggtgc
    11701 gctccttcag caggacgatg ggttggttcg cgggaagctc gacccgcacg ccttcgagcc
    11761 gcatctcgat catcgacgtt ccagggtacg gcctcccccc aggagcggca accgctcagg
    11821 acgacggttc gccgtcgagg tacggcagga gcggagcagg gtcgtcggcg agcgcggtcg
    11881 ggtcggcgac cgcaacgatg aagggcaggt tccggaaccg ctcccccacg tccatcccgt
    11941 acccgacgac gaagcggtcg gggcagtcga acccgcggta gcggatctcg aggggcgtga
    12001 tccggcggac cgacttgtcg agcagcgtgc agacctcgac cgaagctggg ttgcgggccg
    12061 cgagcacgga cagcaggtag gccatcgtga gcccggtgtc gacgatgtcc tcgacgacga
    12121 tcacgtcgcg gccgccgatg tcgcggtcga ggtccttcat gatccgcacg acgcccgagg
    12181 gcgtgccgcc gccgtaggac gagatcgaga tgaaatcgac ggagatgttc gtcccgacca
    12241 cgcgggcgag gtccgcgagg aacgtcgccg cccccttcag gaccccgacg aggatcggct
    12301 cgcgtcccgc gtactcctcg gtgatccgcg ccccgagcgc acgcacgcgg tcgcggatct
    12361 cccgcgcccc gtacaggagc tccacgcccg gatccaccgc cctatcgtac cggcgcctcc
    12421 gcggcccagg ccgtgaagaa ggcccggacg gcgtcgagcg ctcgtcgcca ccaggccggc
    12481 gggaccacgg gggcgggctc gacggccgcc gccgcgcgga ccgggacctc ggccacgacc
    12541 gctcccccgg ccgtatacgt caccgtcccg acctcctgtc cctcgcggac cgggagtcgg
    12601 aggccgggct cgagccggac ctcccgctcg acctcctccc cacggcgcag gacggcactc
    12661 gcctcctccg cgaggaccag gggcacgcgc cggccagcga tcccggccgc cggcagcgac
    12721 ccacccgccg gggagagcgt ccggcgctcg tacgcgcgga acccgtggtt cagcagcgcg
    12781 gcggtgtcgc tgaacgcctc tcgcggcgag ccgaggacga cggcgacgag gtgcaggtcg
    12841 cgacgctcgg cggtcgccac gaggcagtag cccgcagcgg ccgtgtaccc ggtcttcacg
    12901 ccggtcgctc cgcggtagag ccacaggagc acgttgcggt tctggacccg gcggtcgggg
    12961 cccgcatccg ccggcacccg gtggcgcttc gtggcgacga tctcggcgaa gcgttcgtgt
    13021 cgcatcgcct cgacggtcaa ccgcgcgagg tcgcgcgccg tcgaccgccc gcggtcgtcg
    13081 aggccgttcg ggctcgcgaa ccgcgtgcga cgcagaccga gggcgcgcgc ccgacggttc
    13141 atgcggcgga cgaaccgctc gacggagccg gagacgtgct ccgcgagggc gacggcggcg
    13201 tcgttcgccg actggagcat cagcgcctgg agcagctggc gaacctcgat gcgctccccc
    13261 gcgcggagcc cgaggcgcgc accgggctcg gcggcggcgc gctcgctcac gacgacgacc
    13321 tcgaagggcg tggtcgactc gaggacgatc agcgccgtca tgaccttcgt cgtgctggcg
    13381 atcgggagac gccggccgcc gcggcgctcg aacagga
//
LOCUS       DATLGG010000042         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_97538, whole genome shotgun sequence.
ACCESSION   DATLGG010000042 DATLGG010000000
VERSION     DATLGG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97538"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VM638_01110"
     CDS             <1..208
                     /locus_tag="VM638_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVM11061.1"
                     /translation="RGSVPEVVADAETGFVLPVDGFGEAAADALRRVGDIDPKACRRR
                     VEERFSKEAMVTGYERAYERALAT"
     gene            219..731
                     /locus_tag="VM638_01115"
     CDS             219..731
                     /locus_tag="VM638_01115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVM11062.1"
                     /translation="MEHISEAIAAARVYLEAHPDEARYTDTEARAVLEEDLRFRVTGP
                     GEESVTTDMSTAVGGGGEHPSPGWLFRAALASCNGTLIAMRAAEEGIRLTELEVLVDS
                     ESDDRGIFGMDPQTPAAPLGIRVRVRVAAEGVDDARLREVVRWGVDHCPVCDAAKRPV
                     EVTTEIDTSA"
     gene            complement(741..2810)
                     /locus_tag="VM638_01120"
     CDS             complement(741..2810)
                     /locus_tag="VM638_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVM11063.1"
                     /translation="MKRMLLALVLTSLATAALPAGAGAVDGGGSLYRIPFASGPIDPA
                     ADLEPLDPVGRDLVLVQFDAFGARGLVQAVAATGAALVQPLAPVSYLVWADAEQTRSI
                     RAIRGVRWAGVLPPAARISPSVTAATTSLRVTVIGTGGLEGLDPAIVDPRGFTSLAGA
                     VVKVAGGVGAARRLAANPRVYSVAADDDRPETRDEQSAQIVAAGTKVFLAPGYKDFLT
                     RIGADGSGVVVSHIDTGADLLHPDLSGRPHVCDGYGTYGSNQCAATGTNDLDGHGTHT
                     LGIILGSGTTPFRDSQQFLQGQGMAPGATAVVANAIGLGSNAFSGGYRGPYQVASRRG
                     ATVSGNSWGPSATPRGYDANTRTFDSIVRDADTGTPGDQPMTLVFSIMNGSGGTSTQG
                     SPDEGKNILGVGASGSRGGRTPDDLCTCTAHGPALDGRLLPDLVAPGQSVLSTAAAVG
                     ASCRTPFVGVMPSPIHGTCTGTSMASPHVTGGTAVFTEWYRKRHDGQTPSPALVKAAF
                     VNGADDLAGNRNANGAVMGPIPNNQQGWGRFNLGNVMDAWIAGTVHIDQTVVFDAAGA
                     AHTLKVEPRDPTKPLKVTLAWTDALGHGLGGSIPAWVNDLDLSVRSPDDTVWRGNVFA
                     QGFSAPGGSHDRRNNVENIFLAQPGGGTFEVRVSAVNLLGDGIPSRDGLTDQDFALVI
                     TNARLAG"
     gene            complement(2847..>3383)
                     /locus_tag="VM638_01125"
     CDS             complement(2847..>3383)
                     /locus_tag="VM638_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVM11064.1"
                     /translation="LRYFVPRRRHRRKAKRPGRAADLVRLLEADGWIHLRNRTHDLDD
                     EGIELVGLDDPHIHRGDPRVALRRHPERFGLAVVHSPDPGPELVALGWDLVVSGHTHG
                     GQVNLPFVGALVTNCSLPPRMAKGLFRLGSGYLHVSPGLGTGKYAPFRFLCPPEAALI
                     ELGRKRTGKKGSGPSTSN"
BASE COUNT          452 a         1192 c         1270 g          469 t
ORIGIN      
        1 ccgcggctcc gttccggagg tggtcgccga cgccgagacc ggcttcgtcc tgccggtcga
       61 cgggttcggc gaggccgccg ccgacgcgct ccgccgtgtg ggcgacatcg acccgaaggc
      121 ctgccggcga cgggtcgagg agcggttctc gaaggaggcg atggtcactg gctacgagcg
      181 cgcctacgaa cgcgcgctcg cgacgtgagg agggcgagat ggagcacatc agcgaggcga
      241 tcgcagcggc gagggtctat ctcgaggccc atcccgacga ggctcgctac accgacacgg
      301 aggcgcgcgc cgtgctcgag gaggatctgc gcttccgcgt gaccgggccc ggcgaggagt
      361 ccgtgaccac cgacatgtcg acggctgtgg gcggcggcgg ggagcatccc tcgcccgggt
      421 ggctcttccg cgcggcgctc gcgtcttgca acgggacgtt gatcgcgatg cgcgccgccg
      481 aggagggcat ccgcctcacc gagctcgagg tgctcgtcga cagcgagtcg gacgaccgtg
      541 gcatcttcgg catggacccg cagacccccg cggcaccgct cggcatccgc gtgcgggtgc
      601 gggtcgcggc cgagggcgtc gacgacgcgc gcctgcgcga ggtggtccga tggggcgtgg
      661 accactgccc ggtctgcgac gcggcgaagc gcccggtcga ggtgacgacc gagatcgaca
      721 cctcggcctg acccggcgcc tcagccggcg aggcgcgcgt tcgtgatcac cagcgcgaag
      781 tcctgatccg tcaagccgtc ccggctcggg attccgtcac cgagcaggtt cacggctgag
      841 acccgcacct cgaacgtgcc gccgccgggc tgcgcgagga agatgttctc gacgttgttc
      901 cggcgatcgt ggctcccgcc cggcgccgag aacccctgtg cgaacacgtt cccccgccag
      961 accgtgtcgt cgggcgagcg gacgctgagg tcgagatcgt tcacccatgc cgggatcgag
     1021 ccgccgagac cgtgtccgag cgcgtcggtc cacgcgagcg tcaccttgag gggcttcgtc
     1081 gggtcgcgcg gctcgacctt cagcgtgtgc gcggctccgg ccgcatcgaa gaccacggtc
     1141 tggtcgatgt gcaccgtgcc ggcgatccac gcgtccatca cgttgccgag gttgaaccgc
     1201 ccccacccct gctggttgtt cgggatcggc cccatcaccg cgccgttcgc gttgcggttc
     1261 ccggcgaggt cgtccgcccc attcacgaac gccgccttca cgagcgcggg ggacggggtc
     1321 tggccgtcgt ggcgcttgcg gtaccactcg gtgaacacgg ccgtcccgcc ggtcacgtgc
     1381 ggcgaggcca tcgacgtgcc ggtgcaggtc ccgtggatcg gcgacggcat gacgccgacg
     1441 aacggcgtcc ggcaggaagc cccgacggcg gccgccgtcg acaggacgct ctggccgggg
     1501 gcgacgaggt cgggcaggag tcgcccgtcg agcgccggcc cgtgtgccgt gcacgtgcac
     1561 aggtcgtcgg gggtgcgccc gccgcggctg cccgaggctc cgacgccgag gatgttcttg
     1621 ccctcatcgg ggctcccctg cgtcgaggtc ccgccgctgc cgttcatgat cgagaacacg
     1681 agcgtcatcg gctggtcccc gggcgtcccg gtgtccgcgt cccgcacgat cgagtcgaag
     1741 gtgcgcgtgt tcgcgtcgta gccgcgcggg gtcgccgatg gaccccagga gttgccggag
     1801 accgttgcgc cgcgccgcga ggccacctga tacgggccgc ggtagccgcc gctgaaggcg
     1861 ttcgagccga ggccgatcgc gttcgcgacc accgcggtcg cgcccggggc catcccctgg
     1921 ccctggagga actgctggct gtcgcggaac ggcgtcgtgc ccgacccgag gatgatgccg
     1981 agggtgtgcg tcccgtgccc gtcgaggtcg ttcgtgcccg tcgccgcgca ctggttcgac
     2041 ccgtaggttc cgtagccgtc acagacgtgg ggacgcccgg agaggtcggg gtgaagcagg
     2101 tcggccccgg tgtcgatgtg ggagaccacc acgccgctcc cgtcggcgcc gatccgggtg
     2161 aggaagtcct tgtagccggg ggcgaggaag accttcgtcc cggcggcgac gatctgcgcg
     2221 gactgctcgt cccgcgtctc gggccggtcg tcgtcggcgg cgaccgaata gacgcgaggg
     2281 ttggcggcca ggcgcctcgc cgctccgacg ccgcccgcga ccttcacgac cgcgcccgcc
     2341 agcgaggtga agcctcgagg atcgacgatc gcggggtcga gaccttcgag accgcccgtc
     2401 ccgatgacgg tgacccggag cgaggtcgtc gccgccgtga ccgagggaga gatccgcgcg
     2461 gccggcggca gcacgcccgc ccagcgcacg ccgcggatcg cccggatcga gcgcgtctgc
     2521 tcggcatctg cccacacgag gtacgacacg ggcgccagcg gctggacgag cgctgcgccc
     2581 gtcgccgcga cggcctgcac gagcccgcgg gcgccgaagg cgtcgaactg cacgaggacg
     2641 aggtcgcgcc ctaccgggtc gagtggctcc aggtcggcgg ccgggtcgat cgggccgctg
     2701 gcgaacggga tccggtagag gctccctccg ccgtccaccg ccccggcccc ggcgggcagg
     2761 gctgcggtgg cgagcgaggt caggacgagg gcgagcagca tccgcttcat cggtctccct
     2821 tccgcgtgga cgtgccgtat ccagatctag ttcgaggtgg agggccccga tcccttcttc
     2881 cccgtgcgct tcctgccgag ctcgatcagg gcagcttcgg gggggcagag gaaccggaac
     2941 ggcgcgtact tgcccgtccc gagtcccggc gagacgtgga ggtagccgga cccgaggcgg
     3001 aagagcccct tggccatccg cgggggcagc gagcagttgg tcaccagcgc cccgacgaac
     3061 gggaggttga cctgcccgcc gtgggtatgg ccggagacga cgagatccca cccgagcgcc
     3121 acgagctcgg gcccggggtc gggcgagtgc accacggcga ggccgaagcg ctcggggtgg
     3181 cggcgcaggg cgacccgcgg gtcaccacgg tggatgtgcg ggtcgtcgag cccaacgagc
     3241 tcgatcccct cgtcgtcgag gtcgtgggtc cggttgcgga ggtggatcca cccgtcggcc
     3301 tcgaggagcc ggacgaggtc cgcggcccgc cccggtcgct tcgccttccg ccggtgcctc
     3361 cggcgcggca cgaagtagcg cag
//
LOCUS       DATLGG010000043         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_130065, whole genome shotgun sequence.
ACCESSION   DATLGG010000043 DATLGG010000000
VERSION     DATLGG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130065"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VM638_01130"
     CDS             complement(<1..323)
                     /locus_tag="VM638_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11065.1"
                     /translation="MGFFSKRGRDPNDFVPNRDMRIVVLDGDRPVEEVEDGKPYRLRT
                     EGLHEWGRPELEITGVPLPALESAAVLLNMAAGYMVAVRPVKSGETWAAEDHGRLIVV
                     ARMFEA"
     gene            complement(393..1169)
                     /locus_tag="VM638_01135"
     CDS             complement(393..1169)
                     /locus_tag="VM638_01135"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HVM11066.1"
                     /translation="MDGLDPGEVRRAADVLRSAARVLFVTGAGISADSGLPTYRGIGG
                     LYERDTTEEGYPIEVALSGAMLAGNPALCWKYIAQIERACRGARFNAAHAFLAEHEKR
                     VPGTWILTQNVDGFHLDAGSRNVIEVHGNVRRLHCTACEWETEVADYSSLEIPPKCPA
                     CAGLVRPRVVLFGEMLPDDAVRALQRELASGFDAVVAIGTTAVFPYIAGPVIAAARAG
                     LPTIEVNPGDTTISDAVSIRLRAGAVAALTALGDALGAEV"
     gene            1257..2108
                     /locus_tag="VM638_01140"
     CDS             1257..2108
                     /locus_tag="VM638_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11067.1"
                     /translation="MRALLLPFLSAALLAACTTSGGTGDDDDDGSSPTPTPTWSVLMT
                     GDWTLPAYSEDTADVHQKTMDRTVYIGGIRPIAPLGTHHTVLYLGSPAGTNMIYASGV
                     GTGEIVFPEGVGLKLNQGQLISLELHIFNTSDTELSGTSGVEILEVSAADVEQEADIF
                     LPGPLSLSIPPSQTHTVTDPCVLNAPANFFAIFPHMHQLGTHFKTTLTINGTTRVLHD
                     APYYFDQQYFTPFTPIAMGAGDTITTECTWNNTTTQTVEWGESSNEEMCFSILYRWPA
                     QAATFCF"
BASE COUNT          341 a          793 c          732 g          308 t
ORIGIN      
        1 gcctcgaaca tccgcgcgac gacgatgagc cggccgtggt cctcggcggc ccaggtctcg
       61 ccgctcttca ccggccgcac ggcgaccatg tagccggccg ccatgttcag cagcaccgcc
      121 gccgactcga gggcgggaag cggcacgccg gtgatctcga gctcggggcg tccccattcg
      181 tggaggccct ccgtgcggag gcggtagggc ttcccgtcct cgacctcctc gacgggccgg
      241 tcgccgtcga ggacgacgat cctcatgtca cggttcggga cgaagtcgtt cgggtcacgg
      301 ccgcgcttcg agaagaaacc catcgctcac ctccaaaggc gacggaccct accgctcatt
      361 gacatccgtg tcaatatact tgacgcgccg cgtcacactt ccgcgccgag ggcgtcgccg
      421 agcgccgtga gcgcggcgac ggcgccggcc cggaggcgga tggagaccgc gtcggagatc
      481 gtcgtgtcgc cggggttcac ctcgatcgtc gggaggccgg cgcgcgccgc cgcgatcacc
      541 gggccggcga tgtaggggaa gacggcggtc gtcccgatcg cgacgacggc gtcgaagccg
      601 ctcgccagct cgcgctgcag cgcgcgaacc gcgtcgtcgg ggagcatctc gccgaacagc
      661 acgacgcgcg gccggacgag gccggcgcag gccggacact tcggcgggat ctcgagggag
      721 gagtagtcgg cgacctccgt ctcccactcg cacgccgtgc agtggaggcg ccggacgttc
      781 ccgtgcacct cgatgacgtt gcgcgacccg gcgtcgagat ggaagccgtc cacgttctgc
      841 gtgaggatcc aggtcccggg cacccgcttc tcgtgctcgg cgaggaacgc gtgcgcggcg
      901 ttgaagcgag ccccccggca cgcgcgctcg atctgggcga tgtacttcca gcaaagggcc
      961 gggttcccgg cgagcatggc gccggagagc gcgacctcga tcgggtaccc ctcctccgtc
     1021 gtgtcccgct cgtagaggcc gccgatcccg cgataggtcg gcaggcccga gtccgcggag
     1081 atgccggccc ccgtgacgaa gagcacccgg gccgcggagc gcagcacgtc ggcggcgcgc
     1141 cggacctcgc cgggatcgag accgtccatg gggaggattc tctcagagcc ccgtccgcga
     1201 gggccagccg ccgcatccgg ttctccccgc gaagccgggt gcgctaccct cggggcatgc
     1261 gcgccctcct cctgccgttc ctctccgccg cgctcctggc cgcctgcacg acctcgggag
     1321 gcaccggcga cgacgacgac gacggctcgt cgccgacgcc gacgccgacc tggtcggtgc
     1381 tcatgaccgg agactggacg ctcccggcgt acagcgagga caccgccgac gtccaccaga
     1441 agacgatgga ccgcaccgtc tacatcggcg gcatccggcc gatcgcgccg ctcggcacgc
     1501 accacaccgt gctgtacctc ggctcccccg cggggacgaa catgatctac gcctcggggg
     1561 tcgggaccgg cgagatcgtg ttccccgaag gggtcggcct caagctgaac cagggacagc
     1621 tcatcagcct cgagctccac atcttcaaca cgagcgacac ggagctctcc ggcacgtcgg
     1681 gggtggagat cctcgaggtc tccgcggccg acgtcgagca ggaggccgac atcttcctgc
     1741 cgggcccgct gtcgctgtcg atcccgccct cgcagaccca cacggtgacc gacccgtgcg
     1801 tgctcaacgc gccggcgaac ttcttcgcga tcttcccgca catgcaccag ctcgggacgc
     1861 acttcaagac gacgctgacg atcaacggca ccacgagggt cctgcacgac gcgccgtatt
     1921 acttcgacca gcagtacttc acgcccttca cgccgatcgc gatgggcgcc ggcgacacga
     1981 tcaccaccga gtgcacctgg aacaacacga ccacgcagac ggtggagtgg ggcgagagct
     2041 cgaacgagga gatgtgtttc tcgatcctct accgctggcc cgcgcaggcg gcgaccttct
     2101 gcttctgatc agccgctcgc cgacttcgcg gtgcgtgcga ggtcggtgag cagctcctgg
     2161 aggaccggca ggtc
//
LOCUS       DATLGG010000044         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_144419, whole genome shotgun sequence.
ACCESSION   DATLGG010000044 DATLGG010000000
VERSION     DATLGG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144419"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VM638_01145"
     CDS             complement(<1..776)
                     /locus_tag="VM638_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM11068.1"
                     /translation="MPGSGPRRSLSVPRRFGVVLAFILAGAAACTTPPGADDGPPAGP
                     GGTIPSSLRIALTSSGSLDPAELDTPESQLIADQIFDGLVGYDPGTGAVVPAVAETWD
                     VRDGGRRFVFRLRGDATFHDGSPVTADRFVFAWNRLADPGRAAPFAFLLESIAGYRAF
                     HAELEAPSLTGVRAANPRTLEVQLTRPWPDFIAVLGHPALSPLPASAEDAGFALQPVG
                     NGPYRVATQAVPGSPVTLERHDAYHGPPAVVPNVVFAFHGT"
     gene            complement(757..984)
                     /gene="secG"
                     /locus_tag="VM638_01150"
     CDS             complement(757..984)
                     /gene="secG"
                     /locus_tag="VM638_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVM11069.1"
                     /translation="MLTVTLIVINVISSLLLILFVLLHAGKGGGLSDMFGGGGSSMSG
                     GTAVERNLDRITVVTAIVFSATTFWLAWQWT"
     gene            complement(984..1991)
                     /locus_tag="VM638_01155"
     CDS             complement(984..1991)
                     /locus_tag="VM638_01155"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HVM11070.1"
                     /translation="MQPLLKVSGLKKHFPIHRGIVFQRRVGEVRAVDGIDLEVYPGET
                     IGLVGETGCGKSTTARLVARLLDPTDGQILFGGRDITRASRRDLRPLRREMQMIFQDP
                     YASLNPRKTVGSIIGEPYRIHRTVPKGRVKDEVQQLMELVGLSPEHYNRYPHEFSGGQ
                     RQRIGVARALALRPKLIICDEPVSALDVSIQAQILNLLEDLQEEFNLTYLFIAHDLSV
                     VKHMSDRVAVMYLGKIVEIAPGETLYENPKHPYTGALLSSVPIPDPRLAAEKRRIPLQ
                     GDVPSPIDPPPGCRFHPRCPNARFPTCRESDPPLTSHHRGQYAACHFPLEDRAVIESD
                     R"
     gene            complement(2039..3052)
                     /locus_tag="VM638_01160"
     CDS             complement(2039..3052)
                     /locus_tag="VM638_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM11071.1"
                     /translation="MAEPLLEVRDLRVHFPTDDGLVKAVDGVSFSIDEGETLGVVGES
                     GSGKSVTFLTVMGLIRRQQAVIEGEVLFRGENLLVASEETMRSVRGKQLSMIFQDPMT
                     SLHPFYRVGDQIVEAIRAHERVGKHDAMERTVEMLRRVGIPQPDERARQYPHEFSGGM
                     RQRAMIAMALCLNPGLLIADEPTTALDVTVQAQILELIDRLKDEFGTAVALITHDLGV
                     VAEHCDDIQVMYAGKIVEAGTREDIYYNAHHPYTWGLLRSITRIDQPKEERLEPIRGL
                     PPSLIFVPPGCPFHPRCPYVMDVCVTETPPLLATDGRHASACHLSQEEKERIFREEVA
                     VRR"
     gene            complement(3052..>3377)
                     /locus_tag="VM638_01165"
     CDS             complement(3052..>3377)
                     /locus_tag="VM638_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM11072.1"
                     /translation="LGEDYIRTARAKGLSERRVVYRHGVRSALTPVVTMLGLDIGIVL
                     GGAVITETVFNLPGIGQYVIDALFVADFPVVMGVTIFGAFFIIVGNLLVDVIYAFLDP
                     RVRYS"
BASE COUNT          529 a         1119 c         1234 g          495 t
ORIGIN      
        1 gtcccgtgga aggcgaagac gacgttgggg acgacggccg gcggtccgtg gtacgcgtcg
       61 tggcgctcga gcgtcaccgg cgaccccggg acggcctggg tcgcgacccg gtacgggccg
      121 ttgcccaccg gttgcaacgc gaacccggca tcctccgcgc tcgcgggcag gggggagagc
      181 gccgggtgcc cgaggaccgc gatgaagtcc ggccaggggc gggtcagctg gacctcgagc
      241 gtgcgagggt tcgccgcgcg cacaccggtg agggacgggg cctcgagctc ggcgtggaag
      301 gcacggtacc cggcgatcga ctcgagcagg aacgcgaacg gcgccgcgcg cccggggtcg
      361 gccagccggt tccacgcgaa gacgaatcgg tctgcggtga cgggggaccc gtcgtggaag
      421 gtcgcgtcgc cccgcagccg gaacacgaac cgccgtccgc cgtcgcggac gtcccacgtc
      481 tcggcgacgg ccgggaccac ggcgccggtt ccggggtcgt agccgacgag accgtcgaag
      541 atctggtcgg cgatcagctg cgactccggg gtgtcgagct cggccgggtc gaggctcccc
      601 gaggaggtca acgcgatccg gagcgaggac gggatcgttc ccccggggcc ggcaggaggg
      661 ccgtcgtccg ctccgggagg cgtcgtgcag gcagccgcgc cggcgaggat gaaggcgagg
      721 acgacgccga agcgccgggg gacgctcagc gagcgtctag gtccactgcc aggcaagcca
      781 gaacgtcgtc gcgctgaaga cgatggcggt gacgaccgtg atccggtcga ggttccgctc
      841 gacggccgtc cctccggaca tgctcgagcc gccgccgccg aacatgtcgg agaggccgcc
      901 gcccttcccc gcgtgcagga gcacgaagag gatcaggagg aggctcgaga tgacgttgat
      961 gacgatcagc gtgaccgtga gcatcagcga tcagactcta tcacggcgcg atcctcgagc
     1021 gggaagtgac acgccgcgta ctgccctcgg tggtgcgacg tcaggggcgg gtcgctctcc
     1081 cggcacgtcg ggaagcgcgc gttcgggcac cgcgggtgga accggcagcc gggcgggggg
     1141 tcgatcggcg agggcacgtc gccctgaagc gggatccggc gcttctccgc cgcgaggcgc
     1201 ggatcgggga tcgggaccga ggagaggagc gcgcccgtgt aggggtgctt cgggttctcg
     1261 tagagggtct cccccggcgc gatctcgacg atcttcccga ggtacatcac cgcgacgcgg
     1321 tcgctcatgt gcttgacgac cgagaggtcg tgggcgatga aaaggtacgt caggttgaac
     1381 tcctcctgga ggtcctcgag caggttcagg atctgggcct ggatcgagac gtcgagggca
     1441 gagaccggct cgtcgcagat gatgagcttc ggccgcaggg cgagggcgcg cgcgaccccg
     1501 atgcgctggc gctggccccc cgagaactcg tgcgggtagc ggttgtagtg ctcggggctc
     1561 aggcccacca gctccatcag ctgctggacc tcgtccttga cccggccctt cggcacggtc
     1621 cggtggatcc ggtagggctc gccgatgatg ctcccgacgg tcttgcgggg gttcagcgac
     1681 gcgtagggat cctggaagat catctgcatc tccctccgga gcggccgcag gtcgcggcgg
     1741 ctcgcccggg tgatgtcgcg cccgccgaag aggatctgcc catcggtcgg gtcgaggagg
     1801 cgggcgacga ggcgcgccgt ggtggacttg ccgcagcccg tctcgcccac gaggccgatc
     1861 gtctcgcccg ggtacacctc gaggtcgatc ccatcgaccg cccggacctc ccccaccctg
     1921 cgctggaaca cgatcccgcg gtggatcggg aagtgcttct tcagcccgct gaccttgagc
     1981 agcggctgca tccccggcgg cggtggggcg gcgggatgac cggcgacgtc cgtcacgctc
     2041 accgtcggac cgcgacctcc tcgcggaaga tccgctcctt ctcctcctgg gagaggtgac
     2101 acgccgacgc atggcgaccg tcggtggcga ggagcggcgg cgtctccgtc acgcagacgt
     2161 ccatgacgta cggacagcgc gggtggaagg gacagcccgg cggcacgaag atcaacgagg
     2221 gcgggagccc ccggatcggc tcgagccgtt cctccttcgg ctggtcgatg cgggtgatcg
     2281 agcgcaggag gccccacgtg taggggtggt gggcgttgta gtagatgtcc tcgcgggttc
     2341 cggcctcgac gatcttcccc gcgtacatca cctggatgtc gtcgcagtgc tccgcgacga
     2401 cgccgaggtc gtgggtgatg agcgcgacgg cggtcccgaa ctcgtccttc aatcggtcga
     2461 tcagctcgag gatctgcgcc tggaccgtga cgtcgagcgc ggtcgtcggc tcgtccgcga
     2521 tcagcagccc ggggttgagg cagagcgcca tcgcgatcat cgctcgctgc cgcatccccc
     2581 cggagaactc gtgcgggtac tgccgggcgc gctcgtccgg ttgagggatg ccgacccgcc
     2641 ggagcatctc gacggtccgc tccatcgcgt catgcttgcc cacccgctcg tgcgcccgga
     2701 tcgcctccac gatctggtcc ccaacccggt agaacgggtg gagcgaagtc atcgggtcct
     2761 ggaagatcat gctgagctgt ttgccgcgga ccgagcgcat cgtctcctcg gacgcgacga
     2821 gcaggttctc tccgcggaac agcacctcgc cctcgatcac cgcctgttgt cgacggatca
     2881 gccccatgac cgtgaggaac gtcacgctct tcccggaccc ggattcgccg accacgccga
     2941 gcgtctcccc ttcgtcgatc gagaaggaga cgccgtcgac cgccttcacc agaccgtcgt
     3001 cggtcgggaa gtggacgcgg aggtcgcgca cctcgaggag cggttcggcc atcaggagta
     3061 cctcacgcgg gggtcgagga acgcgtagat cacgtcgacc agcaggttgc cgacgatgat
     3121 gaagaaggcc ccgaagatcg tgacgcccat gacgaccggg aagtccgcga cgaagagcgc
     3181 gtcgatcacg tactgcccga tgcccggaag gttgaagacg gtctcggtga tcacggcgcc
     3241 gcccagcacg atgccgatgt cgagcccgag catcgtgacg acaggtgtga gggccgaccg
     3301 cacgccgtga cggtagacga cccggcgctc cgacaggccc ttcgcacggg ccgtccggat
     3361 gtaatcctcc cccagcg
//
LOCUS       DATLGG010000045         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_50398, whole genome shotgun sequence.
ACCESSION   DATLGG010000045 DATLGG010000000
VERSION     DATLGG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50398"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VM638_01170"
     CDS             <1..421
                     /locus_tag="VM638_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HVM11073.1"
                     /translation="PPRMFCEEDFRPTDSWVEVQDTGRVNTFSICYIRWDMVELDPPE
                     IPAVIEIDGASPGVGFMHKLAEVSPDDVTIGLEVEAVWKPEEEREGSILDIEYFRPRD
                     RKLQTNKPKPFSKRASERRKRGGDAQPKRKKDVAGGA"
     gene            421..885
                     /locus_tag="VM638_01175"
     CDS             421..885
                     /locus_tag="VM638_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HVM11074.1"
                     /translation="MFEKLTDPKDIILKEGHVPIRHRYTPGVAGETFFQALKKNGDFL
                     ASRCPECGRVYCPPRLFCERDFAELTANEEVGPEGTLESFTVGYVGLEGEALDQPVAI
                     GLVRLDGADTLLMHRVIDADELEIGQRVVAVLLPASKRVGSINDIRGFRPSS"
     gene            924..1193
                     /gene="rpsO"
                     /locus_tag="VM638_01180"
     CDS             924..1193
                     /gene="rpsO"
                     /locus_tag="VM638_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007052997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HVM11075.1"
                     /translation="MAITKEAKQQIIGDYRRADDDTGSPEVQVAVLSKRIAELTEHLK
                     THRKDHHSRRGLLKMVGRRRRLLDYLKREDIERYRALIQRLGLRR"
     gene            1375..3699
                     /locus_tag="VM638_01185"
     CDS             1375..3699
                     /locus_tag="VM638_01185"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVM11076.1"
                     /translation="MNVSRTIGDSEITLETGKLALQAHGSVVVTLGQTQVLVTCTTSK
                     PREGIDFFPLTVDVEERMYAAGKIPGGFFRREGRPSETAILTCRLIDRPLRPTFHKDF
                     RQEVHVVCTVLSADQANPYDIPCMIGSGMAASLAGLPFDGPVAAVRVAHIGGRWKAFP
                     TYQELDLATFDMVVAGRRNASGQVDVLMVEAEAPEGAVTRIEEGEQAPTEEVVTEGLE
                     EAKAIIGELIDLQAELVEKAGVQRGEFPSFVDYEEDVLQAVDRFARDRVTEATRIAGK
                     QERNQALDEIKTDLVEHLEQTWGEEEYASRADQVKRAYKSLQQEIMRRRVVDEGVRLD
                     GRKPDEIRKITCEVGMVPRAHGSALFQRGETQVLNVTTLGMLRMTQMIDTIDPEDTKR
                     YMHHYNFPPYSTGETGFMRGPKRREIGHGALAERALMPVVPDEVEFPYALRLVSDVLS
                     SNGSTSMASVCGSTLSLMDAGVPIKAPVAGIAMGLIAEDGKYVTLTDILGAEDALGDM
                     DFKVAGTRDYVTALQLDTKLSGLPADVLSQALKQAKEARLYILDKMLETIPEPRSALS
                     ATAPRVESMQIPVDKIGEIIGPKGKRINEIIALTGADIDIQDDGTVFIGSKEGEGAEA
                     AKKMISEILNPRMPAVGEEFEGTVVKTTTFGAFVNLTPGKDGLLHISKLGRGKRVASV
                     EEVVNVGDKVQVVVREVDPAGKVSLDLAGAEEASDGDAGAETGAGDGPGGEGGERPRG
                     DRPSGERREGRDGRDGERRRRRRPREGREQRRDD"
     gene            3692..4981
                     /locus_tag="VM638_01190"
     CDS             3692..4981
                     /locus_tag="VM638_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVM11077.1"
                     /translation="MTEASSSIRRTEYDSGLRLITERMPAVRSVTLGVWVTIGSRDEE
                     PRIAGASHFLEHLLFKGTRKRTARDIAEAFDTVGGDLNAFTARENTTYYCRILDKDLP
                     MAVEHLADMLLDSVIRPADLDAERQVILEEIHMHDDSPDEVVHDLFSETMWGDHPLGR
                     PVLGTVETVSAVSRDQVRRFWKKHYLPENFAVVAAGNLDHDETARLLAGCMEVGEPVH
                     GQRSRVREAGPVPESPGRAVVRTRETEQAHICIGAPGLARSDPRRFAFGLVNSALGGG
                     MSSRLFQEVREKRGLVYSVFSYHQMYAETGVFTVYAGTTPSRAGEVLDIVTRELADIA
                     EKGVTSEELDRAKTHMKGSLALSLEDSGGRMSRLGKSEIGHGEILSLDQIFERVDAVT
                     TEDARVVAEEVLARPRTVAVVGPFDEGAFDRYAGAPA"
     gene            4978..>5350
                     /locus_tag="VM638_01195"
     CDS             4978..>5350
                     /locus_tag="VM638_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11078.1"
                     /translation="MIRVGVLGALGKMGRIVCRAVAEDPDLNLVAAIDRSRDGDQIGP
                     IIGQSSLDVVVSSQTETLLQAETEVVVDFTRPDQVMSNIRWAVDHAMHIVVGTTGITD
                     ADLEDVDKWLASEGGSSNVIVA"
BASE COUNT          910 a         1819 c         1903 g          718 t
ORIGIN      
        1 gcccccgcgg atgttctgcg aggaggactt ccgcccgacc gacagctggg tcgaggtcca
       61 ggacaccggc cgcgtcaata ccttctcgat ctgctacatc cgctgggaca tggtcgagct
      121 cgacccgccg gagatcccgg cggtcatcga gatcgacggc gcctcgcccg gggtcggctt
      181 catgcacaag ctcgccgagg tgagccccga cgacgtgacg atcgggctcg aggtcgaggc
      241 cgtctggaag cccgaggagg agcgcgaggg ctcgatcctc gacatcgagt acttccgccc
      301 gcgggaccgc aagctccaga cgaacaagcc gaagcccttc tcgaagcggg cctcggagcg
      361 gcgcaagcgc ggcggcgacg cccagccgaa gcgcaagaag gacgtggcgg gaggagcctg
      421 atgttcgaga agctcaccga cccgaaggac atcatcctca aggaaggtca cgtgccgatc
      481 cggcaccgct acacgccggg ggtcgcgggg gagaccttct tccaggccct gaagaagaac
      541 ggcgacttcc tcgcttcccg atgccccgag tgcggccggg tgtactgccc gccgcgcctc
      601 ttctgcgagc gggacttcgc cgagctgacg gcgaacgagg aggtggggcc ggagggcacg
      661 ctcgagtcct tcaccgtcgg ctacgtgggc ctcgaggggg aggcgctcga ccagccggtc
      721 gcgatcggcc tggtccggct ggacggcgcc gacaccctgc tgatgcaccg tgtgatcgac
      781 gccgacgagc tcgagatcgg ccagcgggtc gtggcggtgc tgctcccggc atcgaagcgc
      841 gtcggcagca tcaacgacat ccgcggcttc cggcccagct cgtaggtgga gcccgacgcc
      901 gctgtgctag gctgaagtcc cgtatggcca tcacgaagga agcgaagcaa cagatcatcg
      961 gcgactaccg ccgggccgac gacgacacgg ggtccccaga ggttcaggtc gcggtgctgt
     1021 ccaagcgcat cgccgagctg accgagcacc tcaagaccca ccgcaaggac caccactcac
     1081 gtcgtggcct gctgaagatg gtgggtcgcc gccgccggct gctcgactac ctcaagcgtg
     1141 aggacatcga gcgctaccga gccctcatcc agcggctcgg cctgcggcgc taggaggaag
     1201 gaggcgccac cggaccactt gccccgcacc aacgaggagc ggtggggtcg acggtcggtt
     1261 atcagtagcg ggcggcggat ccacggagcc gaagcccacc actgagaacc ggcccagcgc
     1321 cggcccccgc tcctcgaagc catcggactt cgaggaggta caccagtgga ccccatgaac
     1381 gtctcccgaa cgatcgggga cagcgagatc accctggaga ccggcaagct cgccctccag
     1441 gcgcacggat ccgtcgtcgt gaccctgggc cagacccagg tgctcgtgac ctgcaccacc
     1501 agcaagcccc gcgagggcat cgacttcttc ccgctcacgg tggacgtgga ggagcgcatg
     1561 tacgccgccg ggaagatccc cggcgggttc ttccgccgcg agggacgccc gagcgagacc
     1621 gcgatcctca cgtgtcgtct gatcgaccgc cccctgcgcc cgaccttcca caaggacttc
     1681 cgccaggagg tccacgtggt ctgcaccgtg ctctcggcgg accaggcgaa cccctacgac
     1741 atcccctgca tgatcggctc cggaatggcc gcctcgctcg caggtctgcc gttcgacggc
     1801 ccggtcgccg cggtgcgcgt cgcgcacatc ggcgggcgct ggaaggcgtt cccgacgtac
     1861 caggagctgg acctcgccac cttcgacatg gtggtcgcgg gccggcgcaa cgcctcgggc
     1921 caggtcgacg tgctgatggt cgaggccgag gctcccgagg gcgcggtcac tcgcatcgaa
     1981 gagggtgagc aggctccgac cgaggaggtc gtgaccgaag ggctcgagga ggcgaaggcc
     2041 atcatcggcg agctcatcga cctccaggcc gagctggtcg agaaggcggg ggtccagagg
     2101 ggcgagttcc cctcgttcgt cgactacgaa gaggacgtcc tccaggccgt ggaccgcttc
     2161 gcgcgcgacc gggtgaccga ggcgacgcgc atcgccggca agcaggagcg caaccaggcg
     2221 ctggacgaga tcaagaccga cctcgtcgag cacctggagc agacgtgggg cgaggaggag
     2281 tacgcgtcgc gggccgacca ggtcaagcgc gcctacaagt ccctccagca ggagatcatg
     2341 cgccgccgcg tggtggacga gggcgttcgg ctcgacggcc gcaagcccga tgagatccgc
     2401 aagatcacgt gcgaggtcgg gatggtgccg cgcgcccacg gctcggcgct gttccagaga
     2461 ggggagaccc aggtcctcaa cgtcacgacc ctcgggatgc tgcgcatgac gcagatgatc
     2521 gacacgatcg accccgagga caccaagcgc tacatgcacc actacaactt cccgccgtac
     2581 tccaccgggg agaccggctt catgcgcgga ccgaagcgcc gggagatcgg ccacggggca
     2641 ctcgccgagc gcgcgctgat gccggtggtt cccgacgagg tcgagttccc ctacgcgctc
     2701 aggctcgtct ccgacgtcct gtcgtcgaac ggctctacct cgatggcgag cgtctgcggc
     2761 tcgacgctct cgctgatgga cgcgggcgta ccgatcaagg cgccggtcgc cggcatcgcg
     2821 atgggcctga tcgccgagga cgggaagtac gtgacgctca ccgacatcct cggggccgag
     2881 gacgcgctcg gcgacatgga cttcaaggtc gccgggacgc gtgactacgt gacggcgctc
     2941 cagctcgaca cgaagctctc gggccttccc gccgacgtcc tgtcgcaggc gctcaagcag
     3001 gccaaggagg cgcgtctcta catcctcgac aagatgctcg agacgatccc cgagcctcgc
     3061 tccgcgctgt cggccaccgc gccgcgcgtc gagtcgatgc agatcccggt cgacaagatc
     3121 ggggagatca tcggcccgaa gggcaagcgc atcaacgaga tcatcgcgct gacgggtgcc
     3181 gacatcgaca tccaggacga cggcacggtg ttcatcggct cgaaggaggg cgagggcgcc
     3241 gaggccgcga agaagatgat ctccgagatc ctcaacccca ggatgccggc ggtcggcgag
     3301 gagttcgagg ggaccgtggt gaagaccacg accttcggag ccttcgtcaa cctcacgccc
     3361 ggcaaggacg gcctcctgca catctcgaag ctgggccgcg gcaagcgcgt ggcctcggtc
     3421 gaggaggtcg tcaacgtcgg cgacaaggtc caggtcgtcg tccgcgaggt cgaccccgca
     3481 gggaaggtga gcctcgacct cgccggtgcc gaagaggcat ccgacggcga cgccggtgcg
     3541 gagaccggtg ctggcgacgg tcccggtggc gagggcgggg agcgcccgcg tggcgaccgc
     3601 ccgtcgggcg agcggcggga ggggcgcgac ggtcgtgacg gcgagcgccg ccgccgccga
     3661 cgcccgcgcg agggccgcga gcagcgccgg gatgactgag gcctcctcga gcatccgccg
     3721 aaccgagtac gactccggcc tgcggctgat caccgagcgg atgccggccg tccgctcggt
     3781 gacgctcggc gtctgggtga cgatcggctc ccgtgacgag gagccgcgga tcgccggcgc
     3841 gtcgcacttc ctcgagcacc tgctcttcaa gggcacgcgg aagcgcaccg cccgcgacat
     3901 cgccgaggcg ttcgacaccg tcggcggcga cctcaacgcg ttcaccgccc gggagaacac
     3961 cacctactac tgccgcatcc tcgacaagga cctgccgatg gccgtggagc acctggcaga
     4021 catgctgctc gactcggtga tccgcccggc cgacctcgac gccgagcggc aggtgatcct
     4081 cgaggagatc cacatgcacg acgactcgcc cgacgaggtc gtccacgacc tcttcagcga
     4141 gacgatgtgg ggcgaccacc ccctcgggag gccggtcctg gggacggtgg agaccgtgtc
     4201 ggcggtgagc cgcgaccagg tccgccggtt ctggaagaag cattacctcc cggagaactt
     4261 cgcggtggtg gctgccggca acctcgatca cgacgagacc gcgcggctcc tcgcgggctg
     4321 catggaggtc ggggagccgg tccacggcca gcggtcgcgg gtgcgggagg ccgggccggt
     4381 ccccgagagc ccgggacggg cggtggtgcg gacgcgggag accgagcagg cgcacatctg
     4441 catcggggcg cccgggctcg cgcgcagcga tccgcgccgg ttcgccttcg gcctcgtgaa
     4501 cagcgcactg ggcggcggga tgtcctcccg cctgttccag gaggtgcgcg agaagcgcgg
     4561 cctcgtctac tcggtgttct cctaccacca gatgtacgcg gagacgggcg tgttcacggt
     4621 ctacgcgggg acgacgccga gccgcgccgg cgaggtgctc gacatcgtga cgagggagct
     4681 cgcggacatc gccgagaagg gcgtgacgtc ggaggagctc gaccgggcga agacccacat
     4741 gaagggctcg ctcgcactct cgctggagga cagcggcggg cggatgagcc ggctcgggaa
     4801 gtccgagatc ggccacggcg agatcctctc gctcgaccag atcttcgagc gggtggacgc
     4861 ggtgacgacc gaggacgcgc gcgtggtcgc cgaggaggtc ctggcgcggc cgaggaccgt
     4921 cgccgtcgtg gggcccttcg acgagggtgc cttcgaccgc tacgcgggag cgccggcgtg
     4981 atccgtgtcg gggtcctggg cgcgctcggc aagatgggcc ggatcgtgtg tcgcgcggtg
     5041 gcggaggacc cggacctcaa cctcgtcgcg gcgatcgacc gcagccgcga cggcgaccag
     5101 atcgggccga tcatcggtca gtcgtcgctc gacgtcgtag tctcgtcgca gaccgagacc
     5161 ctcctccagg cggagaccga ggtcgtcgtc gacttcacgc gccccgacca ggtgatgagc
     5221 aacatccgct gggcggtgga ccacgcgatg cacatcgtcg tcgggaccac gggcatcacg
     5281 gacgcggacc tcgaggacgt cgacaagtgg ctggcctcgg agggcgggag ctcgaacgtg
     5341 atcgtggcct
//
LOCUS       DATLGG010000046         5658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_130394, whole genome shotgun sequence.
ACCESSION   DATLGG010000046 DATLGG010000000
VERSION     DATLGG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130394"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VM638_01200"
     CDS             complement(<1..376)
                     /locus_tag="VM638_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVM11079.1"
                     /translation="MLEALEHFMTEVIGDYGYWAVFILMMLESALIPIPSEITMVFGG
                     FLASRGELSFFWVGMTGAVANVVGSWLAYWLGIWGGRPLVERWGKYVFLRPHDIDRAE
                     AWFEKHGEMAVFVSRLLPVIRTF"
     gene            complement(457..918)
                     /locus_tag="VM638_01205"
     CDS             complement(457..918)
                     /locus_tag="VM638_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVM11080.1"
                     /translation="MEATLKRRRAGILAALVATLMLAGPLADQEAQAALRDDRRVMRR
                     LINGERAPRGLVRLRAHSALNKVAQSHTREMASANRLYHNGSLAHDLRHVNWSILGEN
                     VGVGSTLYSLHRAFMNSSGHRANILNRRFRRVGIGVVRARGRTWVTVVFKG"
     regulatory      complement(982..1071)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1251..3617
                     /gene="topA"
                     /locus_tag="VM638_01210"
     CDS             1251..3617
                     /gene="topA"
                     /locus_tag="VM638_01210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HVM11081.1"
                     /translation="MAETKQKSKKTNLVIVESPTKAKTLERYLGPDFKVKASVGHIRD
                     LPRKGLGVDVERNFEPTYEINEEKKKVVADLKSAYQKAKEVWLATDFDREGEAIAYHV
                     AHVLGVEPDRAKRVTFTEITEGAVREAFANPRTIDLRLVEAQQARRILDRLVGYNLSP
                     LLIKKVRRGLSAGRVQSPALRLIVEREREIEAFVSEDYWTIEASLATGAEMDPFAAGL
                     IEVDGAKLARSLDRDPAKRKKGEQVLDEAAAREHAANLERAAYRVAEVRRREVKRSPR
                     PPFTTSTLQQEAARKLGFSTKRTMVVAQQLYEGVELPGEGQVGLITYHRTDSTNMAEQ
                     ALAEIGEVVRAQYGERYALAQPRIFKTKSRGAQEAHEAIRPTKASRLPDAVAASLSKD
                     QAKLYRLIWQRAVASQMQEAILDQVSTDIEGTGDGLPRYLLRATGQTVKFDGFRRVYF
                     EGQDDEPDEDAESRLPELSEDQPLDLREVVPEPHQTQPPPRFTEASLVKELERLGIGR
                     PSTYSSIVSNLIDREYVRLEERRFYAEDVGKVVIEKVLKDRFEEIVDYEFTARMESEL
                     DEIAEGNMRWTQVLDEFYAPFERDLEKAEQELQRSDITSEGTGQTCPECGEGEVVEKL
                     GRFGKFLSCTRYPDCKYTAQLDGSQRPEPKTLEETCPECGKPLVEKVGRFGPFVGCSG
                     YPDCRYIKREEKKIGVTCPKCGEGDLVVKRARRRGGNLFYGCNRYPACDFTVGQRPLP
                     EPCPKCGSLVVVQKDGSAKCTNAECGHVIEAAAVPDAAAGEGGAAEAG"
     gene            3617..5629
                     /gene="tmk"
                     /locus_tag="VM638_01215"
     CDS             3617..5629
                     /gene="tmk"
                     /locus_tag="VM638_01215"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVM11082.1"
                     /translation="MASPADEVAALRGTKPATFGSLLRHPAFSRLWRAMLVSSLGDWV
                     GFVAVTVLVTDPDIAGGNPAFAVSGVMLARLLPSIVFGPFAGVLVDRFDRRKLMVISD
                     ISRGVMYASLPFLGTLPAIYLLSFAIECFSLLWAPARDASVPNLVPRRQLANANTLGL
                     ATTYGTLPLGGVVFTSLTGLAAALGTTIGFLGERPEALAFWLDGLTFGFSAYMISRIP
                     MRGSRPAATGKLGANGAVASFGEGIRYLRGHRFVRAMTIGIVLAFIGVGSVMGLGPVF
                     ARRTLGAGPSGWGVLITAVGIGLGIGMASLGWLQRFVSKHVLFPSAMVATAATGTILA
                     LMPSLTPAAVMTVVVGATAGVTWVTGYTLLHENISDEMRGRTFATLTVLSRLGLFVSL
                     AGFPALAGALGDRQIEIGGRDFVLVGTRLALLVGAVIVLMGGILSRRGLLRARLARLR
                     PLVLRPRLRKVERRGAFVVFEGVEGAGKGTQIRMARDFLMSRGIEVLVTREPGGTDLG
                     ERVREAVLDTSPSGVDPRAETLLFAASRAQHVASVIRPALESGSVVICDRYIDSSLAY
                     QGAGRGLGEQDVLSLNAWATQGLFPDLVILLDVDVEEGLARAGRDRDRIEAEDLSFHA
                     KVAEAYLHIAEEHPERFVVIDASRSPQEVHEKVCEALLRFLENGDS"
BASE COUNT          832 a         1970 c         2007 g          849 t
ORIGIN      
        1 tgaacgtccg gatcaccggc aggagccggg agacgaacac cgccatctcg ccgtgcttct
       61 cgaaccaggc ttccgcccgg tcgatgtcgt gggggcggag gaacacgtac ttcccccacc
      121 gctcgacgag cggccggccg ccccagatgc cgagccagta cgcgagccag gagccgacga
      181 cgttcgcgac cgcacccgtc atcccgaccc agaagaagga cagctccccg cgggaggcga
      241 ggaacccgcc gaagaccatc gtgatctccg acgggatcgg gatcagcgcc gactcgagca
      301 tcatcaggat gaacacggcc cagtagccgt agtcaccgat gacctcggtc atgaagtgct
      361 ccagcgcttc gagcatccgc gcaggctatc cgaaaagcga cgacggcccg gggtcgggtc
      421 cgggccgtcg tctgacggtc gtcgttcgcg cggcagctac cccttgaaca cgaccgtgac
      481 ccaggtccgt ccccgcgcgc gcacgacccc gatgccgacg cgccggaagc gccggttgag
      541 gatgttcgcg cggtggccgg acgagttcat gaatgcgcgg tgcaggctgt agagggtcga
      601 gccgacaccg acgttctcgc cgaggatcga ccagttcacg tgacggagat cgtgggcgag
      661 gcttccgttg tggtagaggc ggttcgcgga ggccatctcg cgggtgtggc tctgggcgac
      721 cttgttgagc gcggagtgag cccggagacg caccaggccg cggggcgcac gctcgccgtt
      781 gatcagccgg cgcatcacgc ggcggtcgtc gcggagggct gcctgcgcct cctgatcggc
      841 gagggggccg gcgagcatga gcgtcgcgac gagggcggcg aggataccgg ccctccggcg
      901 cttcagcgtc gcttccacgt ggcctcccga ccgttcctgg gtcccgccca gggacgcccc
      961 gggagccggg ggccctggcc cttcggtagc tcggccgcct gtcgctcacg tggagcggtg
     1021 aggcccggag ctttgcgtcc cgccgttccc gacggtttgc cctttcggga ccaccttcgc
     1081 cctctcgtat cggcaggaac cagtcagacc ttgagggggg gccatcggcc gttcggggga
     1141 cccccgccat cccgcctccg cagcgatgtt ggcgggggcg ggtaccatgg gcccgtcctc
     1201 cccccaccct tgacggctgg ggtatggctg ggataggtag gcgaacccat atggcagaga
     1261 cgaagcagaa gtcgaagaag acgaacctcg tgatcgtcga gtcgcccacg aaggcgaaga
     1321 cgctcgagcg ctacctcggc ccggacttca aggtcaaggc ctcggtgggc cacatccggg
     1381 accttccccg gaagggcctc ggcgtggacg tcgagcgcaa cttcgagccg acgtacgaga
     1441 tcaacgagga gaagaagaag gtcgtcgcgg acctgaagtc ggcgtaccag aaggcgaagg
     1501 aggtctggct cgccaccgac ttcgaccgtg agggcgaggc gatcgcctac cacgtcgccc
     1561 acgtcctcgg cgtggagccg gatcgcgcga agcgcgtcac cttcaccgag atcaccgaag
     1621 gagcggtgcg tgaggccttc gccaacccgc ggacgatcga cctccgcctc gtcgaggcgc
     1681 agcaggcgcg gcgcatcctc gaccgcctcg tcggctacaa cctgtcgcca ctcctcatca
     1741 agaaggtgcg ccgcggcctc tccgccggac gcgtccagtc gccggcgctc cggctgatcg
     1801 tcgagcgcga gcgggagatc gaggcgttcg tctccgagga ctactggacg atcgaggcga
     1861 gtctcgctac cggcgccgag atggatccct tcgcggcggg gctgatcgag gtggacgggg
     1921 cgaagctggc gcgctccctc gaccgcgacc ccgcgaagcg caagaagggc gagcaggtcc
     1981 tcgacgaggc cgcggctcgc gagcatgccg ccaacctcga gcgtgcggcg taccgcgtgg
     2041 ccgaggtccg gcggcgcgag gtcaagcgct ccccccgccc gcccttcacg acctcgaccc
     2101 tccagcagga ggcggcccgc aagctcggct tctccacgaa gcgcacgatg gtcgtcgccc
     2161 agcagctcta cgagggggtc gagctccccg gcgaggggca ggtcggcctc atcacctacc
     2221 accgcaccga ctcgacgaac atggccgagc aggccctcgc cgagatcggc gaggtcgtgc
     2281 gtgctcagta cggcgagcgc tacgcgctgg cccagccccg catcttcaag acgaagagcc
     2341 gcggcgcgca ggaggcgcac gaggcgatcc gtccgacgaa ggcctcgcgg ctgcccgacg
     2401 ccgtcgccgc cagcctgtcg aaggaccagg cgaagctcta ccggctgatc tggcagcgcg
     2461 cggtcgcgtc gcagatgcag gaggcgatcc tcgaccaggt ctcgacggac atcgagggga
     2521 ccggcgacgg cttgccgcgc tacctcctgc gtgcgaccgg tcagacggtg aagttcgacg
     2581 gcttccgccg cgtgtacttc gaagggcagg acgacgagcc cgacgaggac gccgagtcgc
     2641 ggctgccgga gctctccgag gaccagcccc tcgacctgcg cgaggtcgtg ccggagccgc
     2701 accagacgca gccgcctccg cgcttcaccg aggcctcgct cgtcaaggag ctcgagcgtc
     2761 tcggcatcgg ccgcccgtcc acgtactcct cgatcgtctc gaacctgatc gaccgcgagt
     2821 acgtccgtct cgaggagcgc cggttctacg ccgaggacgt cggcaaggtc gtgatcgaga
     2881 aggtgctgaa ggatcgcttc gaggagatcg tcgactacga gttcacggca cggatggagt
     2941 cggagctcga tgagatcgcc gaggggaaca tgcgatggac gcaggtgctc gacgagttct
     3001 acgcgccgtt cgagcgtgac ctggagaagg ccgagcagga gctccagcgc agcgacatca
     3061 cctcggaggg gaccggccag acctgccccg agtgcggcga gggtgaggtc gtcgagaagc
     3121 tcgggcgctt cggcaagttc ctctcgtgca cgcgctaccc cgactgcaag tacaccgcgc
     3181 agctcgacgg gagccagcgg ccggagccga agacgctcga ggagacctgc cccgagtgcg
     3241 ggaagccgct cgtggagaag gtcggccggt tcgggccctt cgtcggctgc tccggctatc
     3301 ccgactgccg ctacatcaag cgcgaggaga agaagatcgg ggtcacgtgc ccgaagtgcg
     3361 gtgagggcga cctcgtggtg aagcgggcgc gccgccgggg cgggaacctc ttctacggct
     3421 gcaaccgtta cccggcgtgc gacttcacgg tcgggcagcg gccgctgccg gagccctgtc
     3481 cgaagtgcgg cagcctcgtc gtcgtgcaga aggacggcag tgcgaagtgc acgaacgccg
     3541 agtgcggcca cgtgatcgag gccgcggccg tacccgacgc tgccgccggc gagggcggcg
     3601 ccgccgaagc ggggtagtgg ccagcccagc cgacgaggtc gcggcgctcc gcgggaccaa
     3661 gcccgcgacg ttcggcagcc tcctccggca ccccgcgttc tcgcgcctgt ggcgcgcgat
     3721 gctggtctcg tcgctcgggg actgggtcgg tttcgtcgcg gtcacggtcc tcgtcaccga
     3781 tccggacatc gccggtggga accctgcctt cgccgtttcc ggcgtgatgc tcgcgaggct
     3841 cctcccttcg atcgtgttcg gtccgttcgc cggggtgctc gtcgaccggt tcgaccggcg
     3901 gaagctgatg gtcatctccg acatcagccg gggggtgatg tacgcgtcgc tcccgttcct
     3961 cggaacgctg ccggcgatct acctgctgtc gttcgcgatc gagtgcttct cgctcctctg
     4021 ggcgccggcg cgtgacgcca gcgtcccgaa cctggtcccc cgccgccagc tcgcgaacgc
     4081 caacacgctg gggctcgcga ccacctacgg caccctgccg ctcggcgggg tcgtgttcac
     4141 ctcgctcacg ggcctcgccg ccgcgctcgg aacgacgatc ggcttcctcg gggagcggcc
     4201 tgaggcgctc gccttctggt tggacggcct gacgttcggg ttctccgcct acatgatctc
     4261 ccgcatcccg atgcgcggat cgcgtcccgc cgcgaccggg aagctcggcg cgaacggggc
     4321 ggtcgcgtcc ttcggcgagg ggatccgcta cctgcgcggt caccggttcg tgcgcgcgat
     4381 gacgatcggc atcgtcctcg ccttcatcgg cgtggggtcg gtgatgggcc tcgggccggt
     4441 cttcgcgcgg cgcaccctgg gggccggccc ctcggggtgg ggcgtcctca tcaccgccgt
     4501 ggggatcggg ctcggcatcg ggatggcgtc cctcggctgg ctccagcggt tcgtctccaa
     4561 gcacgtgctg ttcccgtcgg cgatggtcgc gaccgccgcg acgggcacga tcctcgcgct
     4621 gatgccatcc ttgaccccgg cggcggtgat gacggtcgtg gtgggcgcga ccgccggcgt
     4681 cacctgggtg acggggtaca cgctgttgca cgagaacatc tcggacgaga tgcgcggacg
     4741 gacgttcgcc acgctgacgg tgctgtcgcg cctcgggctg ttcgtgtccc tcgccggctt
     4801 ccccgcgctc gccggtgcgc tcggcgaccg acagatcgag atcggcgggc gcgacttcgt
     4861 cctggtggga acgcgtctcg cgctcctggt cggggccgtc atcgtgctca tgggcgggat
     4921 cctgtcgcgt cggggcctgc tccgcgcgcg cctcgcccgc ctccggccgc tcgtgctccg
     4981 cccgcgcctg cggaaggtgg agcggcgcgg cgccttcgtc gtgttcgagg gggtcgaggg
     5041 agcggggaag gggacgcaga tccggatggc gcgggacttc ctcatgtcgc gggggatcga
     5101 ggtgctcgtg acgcgcgaac ccggcgggac cgacctgggc gagcgggtcc gcgaggccgt
     5161 gctcgacacg tcaccgtccg gggtcgaccc tcgggccgag acgctgttgt tcgccgccag
     5221 ccgcgctcag cacgtcgcct cggtcatccg accggccctg gagtccggca gcgtggtgat
     5281 ctgcgaccgc tacatcgact cctcgctcgc ctaccagggc gccgggcgcg gcctcggcga
     5341 gcaggacgtc ctctccctga acgcctgggc gacgcagggc ctcttccccg acctcgtgat
     5401 cctgctcgac gtcgacgtgg aggagggact cgcccgagcc ggccgcgacc gggatcggat
     5461 cgaggccgag gatctctcct tccacgcgaa ggtcgccgag gcctacctcc acatcgccga
     5521 ggagcatccg gagcgcttcg tcgtgatcga cgcctcgcgc tccccgcagg aggtccacga
     5581 gaaggtgtgc gaggcgctgc tccgcttcct ggagaacggg gactcgtgag cgtcctcgac
     5641 cgcgtgcccg cccaggac
//
LOCUS       DATLGG010000047         5549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_176519, whole genome shotgun sequence.
ACCESSION   DATLGG010000047 DATLGG010000000
VERSION     DATLGG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176519"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VM638_01220"
     CDS             complement(<1..172)
                     /locus_tag="VM638_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVM11083.1"
                     /translation="MAMSETDAVALLREARTIAVVGLSSRPDRDSNSVARYLQKAGYR
                     IIPVNPREEEILG"
     gene            complement(163..726)
                     /locus_tag="VM638_01225"
     CDS             complement(163..726)
                     /locus_tag="VM638_01225"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVM11084.1"
                     /translation="MPDLAEILRASLGTPVADPVLPEGAPAAVLVPVVLEPEPTVLFT
                     RRTETVRQHKGEVSFPGGSRHDDDPDLLTTALRETEEEVGIPREAVEVLGGLPPTQTF
                     VTGYVIAPYVGLLHVRPEMVASPVEIAEVLEARIDVLLEIEREVPSPGGPHPMMFTYD
                     LENVTVWGATGRILHDLLEVLRKGGWR"
     gene            complement(756..2684)
                     /locus_tag="VM638_01230"
     CDS             complement(756..2684)
                     /locus_tag="VM638_01230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HVM11085.1"
                     /translation="MRIRPFLVSLLAAPVVLTGLIAPSAAAPGGLVSKRPVEALRPPW
                     VPGEVLVAFRDGVSAQRSRRIHDRVGARAVDRIAGPGRLEVARVPDGRSIVEAIAAYE
                     RDPDVEAAEPNLYRYPASVPSDPEFRHLWALRNEGQSHEVTPPAAAEAAGSIGSDIDA
                     MGAWEIETGSPQTVIAIIDSGVDVSHPDLVANLWVNPGESGGGKEINNIDDDGNGCVD
                     DVHGCDFTRATPRGRPLHSDDPHGTHVAGTAAAVTNNGIGIAGVCGGDGTAPGCGIMP
                     VRFLGSRGGTLEDELEAIAYVRQMQLDHPDRRFIINASYGGLHWSRLEREAFRRAGQA
                     GVLSVIAAGNSSVDNDRPWCGERCSPSYPASYRLPSTISVAATNHRDQPGYRTACAAD
                     PANTRNRCAFTSWGRRSVHVAAPGVDIKSTVPGAGYDLLDGTSMAAPHVAGIAGLVRS
                     HRPDLGPTEVRNVIMNSVDADGVPAASGMFSVLWPRSNQPKAGRFTWAAGRVNAARAL
                     AEMDTSPAVQPHDGDIPGAKPLGRAASGQVSWPSDVNDLFAKRLMKGKRYRAVLRVPD
                     GRDHDLFVWDPLTVEVWQGFWRIGNPRMLASSENLTKGADERVTFRARATGTHYFHVS
                     AWYLSKGPYELRVRCLDC"
     gene            complement(2732..3517)
                     /locus_tag="VM638_01235"
     CDS             complement(2732..3517)
                     /locus_tag="VM638_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVM11086.1"
                     /translation="MELGLQGRAALVAGASRGLGRGCAEALAAEGARVAIAARDREAL
                     ERAREEIAAATGGEVAAVVVDVAAEPERFVREAAEAVGGCQILVANAGGPRIGPFEEL
                     SDDDFRAALELNFFSTIRMTRAALPLMREAGYGRVIVIGSSSVRQPIPNLMLSNAVRT
                     GVAGWAKTLSLELAPEGITVNTVVPGRFLTDRVRELIEGRRRRSGLDEDAAIGEEAAA
                     IPMGRFGDPRELGDVVAFLASERAAYITGAAYQVDGGLIRGLP"
     gene            3543..3884
                     /locus_tag="VM638_01240"
     CDS             3543..3884
                     /locus_tag="VM638_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HVM11087.1"
                     /translation="MDTTTAPLQVTEAAAAKARQLAEREGRPQACLRVRVVAGGCSGF
                     SYELSFEDAPAADDHVIERGGFRVLVDPKSAGIVEGSTLEFREAMLGGGFRIENPRAV
                     HECACGDSFAI"
     gene            3920..5065
                     /locus_tag="VM638_01245"
     CDS             3920..5065
                     /locus_tag="VM638_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HVM11088.1"
                     /translation="MGETAGKTQWKDLYHEVVATTLCTGCAACVVVCPPKVLGYEDNV
                     PVHLEDNGVDVCTHGDRGCDICTRACPRFREWEPAIDTTLFGRTRNADEEIGIHSEIL
                     LARATDPRSLAHGQDGGVVSALLIWGLEGGEIDGALTSKLSDERPWDAEPTVVTTPEE
                     VLATAGSRYTYSANPLALTLAMQMGLRQVALVGMSCQASVTGKLEAMRLNKYRRRIAW
                     TLGLLCSKTFTYEGLIEEIIQNRLGLRIEDVVRVNVKGKLLIYTSDGKEHTYPLKDAH
                     AFTRPGCTKCPDFAAEHADISFGGLGQSDGWTLTLIRTDRGADIWARALSDGVVEQRP
                     ATEDPAAVELMRKLAVKQRKRWPRDEMPSAWSVPGLVPPVGDQPADR"
     gene            complement(5081..>5549)
                     /locus_tag="VM638_01250"
     CDS             complement(5081..>5549)
                     /locus_tag="VM638_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11089.1"
                     /translation="NNITTCQNVATLQGTFAPAAGTISVTVPRAVLAQQLGSSLAGAR
                     VIPASPAICEGISVKLSPYVSLCGGSAGSQTGDTILQNDEDVYRFASPSVQVGIVPAG
                     AAPAYTQPGTVGGSPTGYSATLDTSNLAPGQYDVHVRACYGTNCGTAVRQVTL"
BASE COUNT          799 a         2019 c         1949 g          782 t
ORIGIN      
        1 caccgaggat ctcctcttcc cgcgggttca ccgggatgat ccggtagccc gccttctgca
       61 ggtagcgggc cacgctgttc gaatcgcggt ccggcctgct ggacaggccg acgaccgcga
      121 tcgttcgcgc ctcccgcaac agcgcaacgg catccgtctc gctcatcgcc atcctccctt
      181 cctcaggacc tcgagcagat cgtgcaggat ccggccggtc gcgccccaga ccgtcacgtt
      241 ctcgaggtcg taggtgaaca tcatcgggtg cggcccgccc ggcgagggga cctcgcgctc
      301 gatctccagg agaacatcga tccgcgcctc gagaacctcc gcgatctcga cggggctcgc
      361 caccatctcg ggacggacgt ggaggaggcc cacgtacggt gcgatcacgt agccggtcac
      421 gaaggtctgg gtcgggggga gcccacccag gacctccacg gcctcgcgcg ggatcccgac
      481 ctcctcctcc gtctcgcgga gggccgtcgt gagcaggtcg gggtcgtcgt cgtgccgcga
      541 cccaccgggg aaggagacct cgcccttgtg ctgccgcacg gtctcggtcc gacgcgtgaa
      601 caggacggtc ggttcgggtt cgaggacgac cgggacgagg acggccgccg gcgcgccctc
      661 gggcagcacc gggtccgcga ccggcgttcc cagggatgcg cgcaggatct ccgcgaggtc
      721 aggcaccgag gcatcgtagc gagcgggagg cgccgtcagc agtcgaggca gcgcacccga
      781 agctcgtagg gccccttgct gaggtaccac gcgctcacgt ggaagtagtg ggtgccggtg
      841 gcgcgggcgc ggaacgtgac tcgctcgtcc gcccccttcg tcaggttctc gctgctcgcc
      901 agcatccgcg ggttcccgat cctccagaac ccctgccaga cctcaacggt caaggggtcc
      961 cagacgaaca ggtcgtggtc gcgtccgtcg gggacgcgca ggaccgcgcg gtagcgcttc
     1021 cccttcatga gccgcttcgc gaacaggtcg ttcacgtcgc tcggccacga cacctgcccc
     1081 gaggcggcgc ggccgagcgg cttcgcgccc gggatgtcgc cgtcgtgagg ctggaccgcc
     1141 ggcgaggtgt ccatctccgc cagggcacgg gccgcgttca cccgcccggc cgcccacgtg
     1201 aaccgcccgg ccttcggttg gttcgaccgc ggccacagca ccgagaacat gccgctggcg
     1261 gcgggtacgc catcggcgtc gaccgagttc atgatcacgt tccggacctc cgtcgggccc
     1321 aggtcgggcc ggtgcgaccg gaccaacccg gcgatccctg cgacgtgcgg cgccgccatc
     1381 gaggtcccat cgaggaggtc gtagccagcg ccggggaccg tgctcttgat gtcgacgccc
     1441 ggcgctgcga cgtgcaccga gcgccggccc cagctggtga aggcgcagcg gttgcgcgtg
     1501 ttggccgggt cggcggcgca ggccgtccga tacccgggtt ggtcgcggtg gttcgtcgcc
     1561 gccacgctga tcgtcgaggg gagccggtag ctcgccgggt agctcgggga gcagcgctcc
     1621 ccgcaccacg gtcggtcgtt gtcgacgctc gagttgccgg ccgcgatcac ggagaggacg
     1681 ccggcctgcc cggcacggcg gaaggcctcc cgctcgagcc ggctccagtg gaggccgccg
     1741 tagctggcgt tgatgatgaa cctccggtcc gggtggtcga gctgcatctg gcggacgtag
     1801 gcgatcgcct cgagctcgtc ctcgagcgtc ccgccgcggc tgccgaggaa ccgaaccggc
     1861 atgatcccgc acccgggcgc ggtcccgtcc ccgccacaga cgcccgcgat cccgatcccg
     1921 ttgttcgtca cggccgcggc cgtcccggcc acgtgcgtcc cgtgagggtc gtccgagtgc
     1981 aggggccgcc cgcggggggt ggcccgggtg aagtcacagc cgtggacgtc gtccacgcag
     2041 ccgttcccgt cgtcgtcgat gttgttgatc tccttccctc cgccgctctc accgggattg
     2101 acccagaggt tggcgacgag gtccggatgg gagacgtcca cgcccgaatc gatgatcgcg
     2161 atgacggtct gcggagaccc cgtctcgatc tcccaggcac ccatcgcgtc gatgtcgctg
     2221 ccgatgctgc cggcggcctc cgcggctgcc ggtggcgtca cctcgtgcga ctgaccctcg
     2281 ttccggaggg cccacaggtg ccggaactcc gggtcgctcg gcacggaggc gggatagcgg
     2341 tagaggttcg gctcggcggc ctcgacgtcc ggatcgcgct cgtaggccgc gatcgcctcg
     2401 acgatcgaac gcccatccgg gacgcgggcg acctcgagac gtcccggccc ggcgatccga
     2461 tccacggcgc gggctcccac gcgatcgtgg atccgccgcg atcgctgggc ggagacgcca
     2521 tcgcggaagg cgacgagcac ttctcccggc acccagggcg gccgcagggc ctcgaccggc
     2581 cgcttcgaga cgagcccccc gggtgccgcg gcggagggcg cgatcaggcc ggtcaagacg
     2641 accggggcag cgaggaggga gacgaggaag ggacggatgc gcacgcgatc atccacgata
     2701 cggcatgtgt cgggttggag ccggctgttc cttaggggag cccccggatc agccccccgt
     2761 ccacctggta cgccgccccc gtgatgtagg cggcgcgctc ggaggcgagg aacgcgacga
     2821 cgtcgcccag ctcacgtgga tcgccgaacc gccccatggg gatcgccgcc gcctcctcgc
     2881 cgatcgccgc atcctcgtcg agaccgctcc gccggcgtcg gccctcgatc agctcgcgca
     2941 cgcggtcggt gaggaagcgg cccggaacga cggtgttgac cgtgatcccc tcgggcgcca
     3001 gctcgagcga aagggtcttc gcccacccgg cgacccccgt cctgaccgcg ttcgacagca
     3061 tgaggttcgg gatgggctgg cggaccgagc tcgacccgat cacgatgacg cggccgtagc
     3121 ctgcctcgcg catcagcggg agcgccgccc gggtcatccg gatcgtggag aagaaattga
     3181 gctcgagcgc ggcgcggaag tcgtcatcgg agagctcctc gaacggcccg atgcgcgggc
     3241 cgccggcgtt cgccacgagg atctgacacc ccccgaccgc ctcggctgcc tcccggacga
     3301 accgctcggg ctcggcggcc acgtcgacca cgacggccgc cacctcccct cccgtggcgg
     3361 cggcgatctc ttcgcgcgcc cgctcgagcg cctcacgatc gcgcgcggcg atcgccaccc
     3421 gcgcgccctc cgctgcgagc gcctccgcgc agccgcgccc cagcccccgg ctcgcgcccg
     3481 cgacgagcgc cgcccgaccc tgcagtccca attccatgat gggcttgtat catcgagtcg
     3541 cgatggacac cacaaccgca ccgctccagg tgaccgaggc cgcggccgcg aaggcccggc
     3601 agctcgccga gcgcgagggg cgcccccagg cgtgcctgcg cgtgcgggtc gtcgccggtg
     3661 ggtgctcggg cttctcctac gagctgtcct tcgaggacgc gcccgccgcc gacgatcacg
     3721 tgatcgagcg cggggggttc cgggtcctcg tcgacccgaa gagcgccggc atcgtcgagg
     3781 ggtcgacgct cgagttccgc gaggcgatgc tgggcggggg gttccggatc gagaaccccc
     3841 gcgcggtcca cgagtgcgcc tgcggggact cgttcgcgat ctgaccccgt ccgcgaccgc
     3901 cccacctata ctcgaccgcg tgggcgagac cgccggcaag acccagtgga aggacctgta
     3961 ccacgaggtc gtcgccacga ccctctgcac cggatgcgcc gcctgcgtcg tcgtctgtcc
     4021 cccgaaggtg ctcggttacg aggacaacgt gccggtgcac ctcgaggaca acggcgtgga
     4081 cgtctgcacc cacggcgacc ggggctgcga catctgcacc cgcgcatgcc cgcggttccg
     4141 cgagtgggag cccgccatcg acacgacgct tttcggccgc acccgcaacg cggacgagga
     4201 gatcggcatc cactccgaga tcctgctcgc ccgcgcgacc gacccgcgga gcctcgcgca
     4261 cgggcaggac ggcggcgtcg tctcggcgct gctgatctgg ggactcgagg gcggcgagat
     4321 cgacggcgcg ctcacctcga agctctccga cgagcgcccg tgggacgcgg agccgacggt
     4381 cgtcacgacg ccggaagagg tcctcgccac cgccggatcg cgctacacgt actcggcgaa
     4441 ccctctcgca ctgaccctcg cgatgcagat ggggctccgt caggtcgcgc tcgtcgggat
     4501 gtcgtgccag gcgagcgtca ccgggaagct cgaggcgatg cggctcaaca agtaccgtcg
     4561 ccggatcgcg tggacgctcg gtctgctgtg ctccaagacg ttcacgtacg aggggctgat
     4621 cgaggagatc atccagaacc ggctcggtct gcggatcgag gacgtcgtcc gcgtgaacgt
     4681 caaggggaag ctcctgatct acacgagcga cgggaaggag cacacgtacc cgctgaagga
     4741 cgcgcacgcg ttcacacgcc ccgggtgcac gaagtgcccc gacttcgccg cggagcacgc
     4801 ggacatctcc ttcggtggcc tcggccagag cgacggctgg acgctcacgc tgatccgcac
     4861 cgatcgcggc gcggacatct gggcccgtgc cctctcggat ggcgtcgtgg agcagcgccc
     4921 cgcgaccgag gaccccgcgg cggtcgagct gatgcgcaag ctcgccgtca aacagcgcaa
     4981 gcgctggcca cgtgacgaga tgccgtcggc gtggagcgtc cccgggctgg tcccccccgt
     5041 cggggaccag cccgcggacc gctgacggat cccggaagcg ctacagggtg acctgccgga
     5101 ccgcggtccc gcagttcgtc ccgtagcagg cacgaacgtg cacgtcgtac tggccggggg
     5161 ccaggttcga ggtgtcgagc gtcgccgagt agccggtggg gcttccgccg accgtcccgg
     5221 gctgggtgta cgccggcgcg gcccccgcgg gaacgatccc gacctgcacg ctcggggagg
     5281 cgaaccgata cacgtcctca tcgttctgca ggatcgtgtc gccggtctgc gatcccgccg
     5341 acccaccgca cagcgagacg tacggagaga gcttcaccga gatgccctcg cagatcgccg
     5401 gcgaggccgg gatcacccgg gcgcccgcga gcgacgaacc gagctgctgc gcgagcaccg
     5461 cgcgtggcac cgtgacgctg atcgtgccgg cggcgggggc gaacgttccc tggagcgtcg
     5521 ccacgttctg gcacgtggtg atgttgttc
//
LOCUS       DATLGG010000048         2232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_19958, whole genome shotgun sequence.
ACCESSION   DATLGG010000048 DATLGG010000000
VERSION     DATLGG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19958"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2209
                     /locus_tag="VM638_01255"
     CDS             <1..2209
                     /locus_tag="VM638_01255"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HVM11090.1"
                     /translation="SMTESHPAPPPNGGPAARRLLTENVPTVVTELLSRVLAIKEGET
                     FCYSDTEGTVDDRPGYGLGFYFQDTRFLSHFRMRIGGRDPILLSSSAERAYLCYVDVT
                     NPDLYDGSQLTVPQQTLNIRRMRAVAGRIFERIRMKNYNGFPIAFDVELEFAADFVDL
                     FEVRGLTRERRGRQHPPEASGREIRFTYTGEDDVVRTTLISFGQSPEALEVRGDLVVA
                     RFRVEVGPHETKVLSLSVEPNVGDDAPPAKEFDAAVHELRRSYEDWERECTQIRTDNE
                     LFNQLLTQGLRDLRALYTKLGDHGVIAAGIPWFVAPFGRDTLFTSHQLLMLNTRPARD
                     SLQILAELQGTEVDDWRDEEPGKILHEIRKGELARSGYVPHTPYYGTVDATPWFLILL
                     AQYFRWTGDVDFVRGLLPAARKALRWIDEHGDLDGDGFVEYRSRSSRGLRNQGWKDSW
                     DSVVHADGTLAEPPIALVEVQAYVYMAKSRTADLLSALGEHEEAARLRDQAQALRKRF
                     NEVFWMEEEQYFCEALDGDKRQVKSIVSNPGHALYCDALEPEKAEAVAKRLFQPDMFS
                     GWGIRTMSKAATAYNPMSYHNGSVWPHDNAIIAAGLKRYGFVTPTNRVATAMFDAAVH
                     SDYLRLPELFCGFTRRTPSRPVQYPVACSPQAWAAGAPFLLLQAMLGVSALAHENLLT
                     VNKPHLPRWLNWVELRNLRVGESRLTLVFRREGETTGFSLLSKEGSVRVVMEE"
BASE COUNT          352 a          805 c          736 g          339 t
ORIGIN      
        1 gtccatgacc gagtcgcacc ccgctcctcc cccgaacggt ggccccgccg cgcgccgcct
       61 gctgaccgag aacgtcccga cggtcgtcac cgagctcctc agccgggtcc tcgcgatcaa
      121 ggaaggggag acgttctgct actccgacac cgaggggacc gtcgacgacc gccccgggta
      181 cggcctcggc ttctacttcc aggacacgcg gttcctctcg cacttccgca tgcggatcgg
      241 cgggcgcgac cccatcctcc tgtcgtcctc ggccgagcgc gcctacctct gctacgtcga
      301 cgtcacgaac cccgacctct acgacggctc gcagctcacc gtcccccagc agacgctgaa
      361 catccgtcgg atgcgcgcgg tcgccggccg gatcttcgag cggatccgga tgaagaacta
      421 caacggcttc ccgatcgcgt tcgacgtcga gctcgagttc gcggcggact tcgtcgacct
      481 gttcgaggtc cgcggcctga cgcgcgagcg tcgcggccgc cagcatcctc cggaagcctc
      541 cggtcgtgag atccgcttca cctacaccgg cgaggacgac gtggttcgga cgacgctgat
      601 ctccttcggg cagtccccgg aggcgctcga ggtccggggc gacctcgtcg tcgcgcgctt
      661 ccgcgtcgag gtcgggccgc acgagacgaa ggtgctctcg ctgtcggtcg agcccaacgt
      721 cggcgacgat gctccccctg cgaaggagtt cgatgccgcg gtgcacgagc tccgccggtc
      781 gtacgaggac tgggagcgcg agtgcacgca gatccgaacc gacaacgagc tgttcaacca
      841 gctcctgacg cagggcctcc gtgaccttcg cgcgctctac acgaagctcg gcgaccacgg
      901 cgtgatcgcc gccgggatcc cgtggttcgt ggcgcccttc ggtcgcgaca cgctcttcac
      961 ctcgcaccag ctcctgatgc tgaacaccag gcccgcccgc gacagcctcc agatcctcgc
     1021 ggagctccag gggaccgagg tcgacgactg gcgcgacgag gaacccggga agatcctcca
     1081 cgagatccgc aagggcgagc tggcgcgctc gggctacgtc ccgcacaccc cgtactacgg
     1141 cacggtggac gcgaccccgt ggttcctcat ccttctcgcg cagtacttcc gctggaccgg
     1201 cgacgtcgac ttcgtgcgcg ggctgctgcc ggcggcgcgg aaggcgctgc gctggatcga
     1261 cgagcacggc gacctcgatg gcgacggctt cgtcgagtac cgcagccgct cgagccgtgg
     1321 cctccggaac caggggtgga aggactcgtg ggattcggtg gtccatgcgg acggcaccct
     1381 ggccgagccc ccgatcgcgc tcgtggaggt gcaggcgtac gtctacatgg cgaagagccg
     1441 cacggccgac ctgctctcgg cgctcggcga gcacgaggag gcggcacgcc ttcgggacca
     1501 ggcccaggcg ctgcgcaagc gcttcaacga ggtcttctgg atggaggagg agcagtactt
     1561 ctgcgaagcc ctcgacggcg acaagcgcca ggtcaagagc atcgtctcga accccgggca
     1621 cgccctctac tgcgacgcgc tcgagccgga gaaggccgag gcggtggcga agcgtctgtt
     1681 ccagccggac atgttctcgg gctgggggat ccgcacgatg agcaaggccg ccaccgcgta
     1741 caacccgatg agctaccaca acggatcggt ctggccccac gacaacgcga tcatcgcggc
     1801 gggactgaag cgatacggct tcgtcacgcc cacgaaccgc gtcgcgaccg cgatgttcga
     1861 cgcggccgtg cacagcgact acctgcgcct gcccgagctg ttctgcgggt tcacgcgccg
     1921 aacgccgagc cggcctgttc agtacccggt ggcgtgctcg ccccaggcct gggcggccgg
     1981 cgcgccgttc ctcctgctcc aggcgatgct cggggtctcc gcgctcgccc acgagaacct
     2041 gctgaccgtg aacaagcctc accttccgcg ctggctgaac tgggtcgagc tccgcaacct
     2101 ccgggtcggc gagagtcgtc tgacgctcgt cttccgccgg gaaggcgaga cgaccggctt
     2161 ctccctcctc agcaaggagg gcagcgtccg cgtcgtgatg gaggagtagc cgctagcgga
     2221 tgcaggggcc gg
//
LOCUS       DATLGG010000049         2819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_112989, whole genome shotgun sequence.
ACCESSION   DATLGG010000049 DATLGG010000000
VERSION     DATLGG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112989"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="sufB"
                     /locus_tag="VM638_01260"
     CDS             complement(<1..966)
                     /gene="sufB"
                     /locus_tag="VM638_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HVM11091.1"
                     /translation="MASTETEIVQGIGSDYEVKYGFSVPEDYFFKSGRGLSHELVDAI
                     SSHKGEPDWMRKFRHKSLDYFLSRPMPGWGADLSGIDFENIYYYIRPTEKQADRWEDL
                     PADIKDTWDKLGIPEAEKKYLAGVGAQYESEVVYHKLQEDLERKGVIFLDMDSGLREH
                     EDLVKQYFGTIIPQNDNKFAALNSAVWSGGSFIYVPPGVHIEMPLQAYFRINAENMGQ
                     FERTLIIVDEDAYVHYVEGCTAPIYSSDSLHSAVVEIVVKPRGRCRYTTIQNWSNNVY
                     NLVTKRAVAYEDAVMEWVDGNLGSKLTMKYPAIWLMGERARGEVLS"
     gene            complement(966..1754)
                     /gene="sufC"
                     /locus_tag="VM638_01265"
     CDS             complement(966..1754)
                     /gene="sufC"
                     /locus_tag="VM638_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVM11092.1"
                     /translation="MALLELKDLHVALEDGTEIVKGVDLAVDTDQVHAIMGPNGSGKS
                     TLAYALMGHPAYEVTGGQILLDGEDVTELEADERAQRGLFLAFQYPHAVPGVTVTSFM
                     RQAINSIRKAKAGGVDDPISVKEFRTQMLEAMDRLKVPRELAQRYLNEGFSGGEKKRV
                     EILQMAMLKPRIAILDETDSGLDIDALRIVAGGVQELVGPEMGALVITHYQRILNYIQ
                     PDFVHVFVDGRIVAEGGKELAQRLEEEGYEAFAPAAAGATGGEV"
     gene            complement(1818..>2819)
                     /locus_tag="VM638_01270"
     CDS             complement(1818..>2819)
                     /locus_tag="VM638_01270"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVM11093.1"
                     /translation="SYGLGRAIARAAARRGARVVVAARNGEALEQAVREIDELGGEGL
                     AVTADVASQVENETIVERAVERFGRIDTFVACATVTVYAEARELEPEELRRVFDVNFF
                     GRVYGFWAVLPHLRESGGTFVDVNSALAYRGIPLQAAYCSTKASLRTFLESARVELAR
                     EGAGVDISVVLPGAINTPQFDVSRQKVGKQPQPVPPIYQPEAIAEATLHCFENPIREL
                     PIGWGAQKLLWGQKLAPRAGDLVLLKSGWKDQNTGEPKPVDSPDNLFETLPGDRGARG
                     RFSDRSRDTTAWTALRLRPSVRTLLTLLGVGAPVAATIMAGRNGHAPGAVRRLVRIG"
BASE COUNT          402 a          980 c          970 g          467 t
ORIGIN      
        1 cgacaagacc tcgccgcgcg cccgctcgcc catgagccag atcgccgggt acttcatggt
       61 gagcttcgag ccgaggttgc cgtcgaccca ctccatgacg gcgtcctcgt aggcgacggc
      121 gcgcttcgtg acgaggttgt agacgttgtt cgaccagttc tggatcgtcg tgtagcggca
      181 gcggccgcgc ggcttcacga cgatctcgac gacggcggag tgcagcgagt cggaggagta
      241 gatcggcgcg gtgcacccct cgacgtagtg cacgtaggcg tcctcgtcga cgatgatcag
      301 cgtgcgctcg aactggccca tgttctcggc gttgatccgg aagtaggcct ggagcggcat
      361 ctcgatgtgg acgcccggcg ggacgtagat gaaggagccg ccggaccaga ccgcggagtt
      421 gagcgcggcg aacttgttgt cgttctgcgg gatgatcgtc ccgaagtact gcttgacgag
      481 gtcctcgtgc tcgcggagcc cggagtccat gtcgaggaag atcacgccct tccgctcgag
      541 atcctcctgg agcttgtggt agacgacctc ggactcgtac tgcgcgccga cgccggcgag
      601 gtacttcttc tccgcctcgg ggatcccgag cttgtcccag gtgtccttga tgtcggccgg
      661 gaggtcctcc caccggtccg cctgcttctc ggtcgggcgg atgtagtagt agatgttctc
      721 gaagtcgatc ccggagaggt cggcgcccca cccgggcatc gggcgcgaga ggaagtagtc
      781 gaggctcttg tggcggaact tccgcatcca gtccggctcg cccttgtggg acgagatcgc
      841 gtcgacgagc tcgtgcgaga gcccgcggcc cgacttgaag aagtagtcct cggggacgct
      901 gaagccgtac ttgacctcgt agtcggagcc gatcccctgg acgatctcgg tctcggtcga
      961 cgccatcaga cctcacctcc ggtcgcgccc gcggcggccg gcgcgaaggc ctcgtagccc
     1021 tcctcctcga gccgttgcgc gagctccttg ccgccctcgg cgacgatccg gccgtcgacg
     1081 aagacgtgga cgaagtcggg ctggatgtag ttgaggatcc gctggtagtg cgtgatcacg
     1141 agcgcgccca tctccgggcc gacgagctcc tgcacgccgc cggcgacgat gcgcagcgcg
     1201 tcgatgtcga ggccggagtc ggtctcgtcg aggatcgcga tccgcggctt cagcatcgcc
     1261 atctggagga tctcgacgcg cttcttctcg ccgccggaga agccctcgtt gaggtaccgc
     1321 tgcgcgagct cgcgcggcac cttcagacgg tccatcgcct cgagcatctg cgtgcggaac
     1381 tccttcacgg agatcgggtc gtcgacgccg cccgccttcg ccttccggat cgagttgatc
     1441 gcctggcgca tgaagctcgt cacggtcacg ccggggacgg cgtgggggta ctggaaggcg
     1501 aggaagaggc cgcgctgcgc gcgctcgtcc gcctcgagct ccgtgacgtc ctccccgtcg
     1561 aggaggatct gcccgcccgt cacctcgtac gccggatggc ccatcagcgc gtaggcgagc
     1621 gtcgacttcc ccgagccgtt cgggcccatg atcgcgtgca cctgatcggt gtccacggcg
     1681 aggtcgacgc ccttgacgat ctcggtgccg tcctcgagcg cgacgtgcag atccttcagt
     1741 tcgagcagcg ccatctgttc gtgcgtcctc caggcaagag aaatcgccgg aaaccgtccc
     1801 ggctcgtgac accgagtcta gccgatgcga accaggcgcc ggaccgcccc cggggcgtgc
     1861 ccgttgcgcc ccgccatgat cgtcgcggcg accggcgccc cgaccccgag gagcgtcagg
     1921 agcgtccgca ccgacgggcg caggcgcagc gcggtccacg cggtggtgtc gcgcgaccgg
     1981 tcgctgaagc gtccgcgcgc gccccggtcg ccgggcagcg tctcgaacag gttgtccggc
     2041 gagtcgaccg gcttcggctc gcccgtgttc tggtccttcc agccggactt cagcaggacg
     2101 aggtcgccgg cgcgcggggc gagcttctgc ccccagagga gcttctgcgc gccccagccg
     2161 atcggcagct cgcggatcgg gttctcgaag cagtggagcg tcgcctccgc gatcgcctcc
     2221 ggctggtaga tcgggggaac cggttgcggc tgcttcccca ccttctgccg gctcacgtcg
     2281 aactgcggcg tgttgatcgc gccgggtagg acgacggaga tgtcgacgcc ggcgccctcg
     2341 cgcgccagct ccacgcgcgc cgactcgagg aacgtccgca gcgacgcctt cgtcgagcag
     2401 tacgccgcct gcagcgggat cccgcggtag gcgagcgccg agttcacgtc gacgaacgtg
     2461 ccgccgctct ccctcaggtg cgggaggacg gcccagaacc cgtagacccg gccgaagaag
     2521 ttgacgtcga agacgcgccg cagctcctcc ggttcgagct cgcgcgcctc cgcgtacacc
     2581 gtcaccgtcg cgcaggcgac gaacgtgtcg atccggccga agcgctcgac cgcgcgctcg
     2641 acgatggtct cgttctcgac ctggctcgcg acgtcggcgg tgacggcgag cccctcgccg
     2701 ccgagctcgt cgatctcgcg gacggcctgc tcgagcgcct cgccgttgcg cgcggccacg
     2761 acgaccctcg cgccgcgccg ggccgcggcg cgcgcgatcg cgcggccgag gccgtagga
//
LOCUS       DATLGG010000050         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_97806, whole genome shotgun sequence.
ACCESSION   DATLGG010000050 DATLGG010000000
VERSION     DATLGG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97806"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..2709)
                     /locus_tag="VM638_01275"
     CDS             complement(235..2709)
                     /locus_tag="VM638_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HVM11094.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRQQVEEIIGQGQSTPAGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQQVIQLLSGYTGGKEPGAAEQAPQG
                     SMVLDQFGRNLTQLARDGKLDPVIGRAKEIERVMQVLSRRTKNNPVLIGEPGVGKTAI
                     VEGLAQDIVKGEVPETLNGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRGDII
                     LFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDAALERR
                     FQPIDVNEPSVTHTIEILKGLRDRYEAHHRVSFTDDALVAAANLSDRYISDRFLPDKA
                     IDLIDEAGSRIRIRRMTAPPDVREVDEKIADVRMRKESAIDGQDFEAAANLRDEERNL
                     IEERTRREKDWKSGELDILSEVDEEEIAEVLAMWTGIPVYKLTEEETQKLLRMEDELH
                     KRLVNQSEAVTAVSKAIRRTRAGLKDPKRPSGSFIFLGPSGVGMTELSKTLAEFLFGD
                     EDSLIQLDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRKPFSVVLFDEIEKAH
                     PDVFNTLLQILEDGRLTDAQGHTVDFKNTVIIMTSNLGTRDLAKASIGFARQDAEEGV
                     SYEKMKDKVNEELKRSFRPEFLNRIDEVIVFHSLSREHVKDIVDLMTKRVREQLKSKD
                     VEIVLTEAAKELLAEKGYDPALGARPLRRTIQRMVEDPLSEKLLWKEFQAGQTVIVDA
                     RNDEIVFESGASPDAPPIELAGQQES"
     gene            complement(2987..3268)
                     /locus_tag="VM638_01280"
     CDS             complement(2987..3268)
                     /locus_tag="VM638_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11095.1"
                     /translation="MGFKRGLIIGFGVGYVLGTKAGKERYEELREAWDRFMGNPTVRS
                     VVERGREVVESGAQKSLHVVEEGVRKASGTVKQKLDGEEEEGWEPVKKS"
     gene            3464..>3617
                     /locus_tag="VM638_01285"
     CDS             3464..>3617
                     /locus_tag="VM638_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11096.1"
                     /translation="MYRRYREDPASVDEAWAEFFEGYRPRGAAPPEPQPAPTRTQAPT
                     APAPDAA"
BASE COUNT          471 a         1284 c         1227 g          635 t
ORIGIN      
        1 gaagaggagt tgctcgagag gctgcgtcag gcgggcgtct tccggctcga cgggatcgcg
       61 ctcgcgatca tcgaaccgac cggcggcatc tcgctgctgc agcgcggcga ggaggcggat
      121 gagctcatgc tgcgcaacgt ccggcgctga ccccaggatg ccgaaggccc ggcaaccctg
      181 cggctgccgg gccgtcggcc ttaccgcccg cgagcgtccc cgcgggcggg gtcttcagga
      241 ctcctgctgg cctgccagct cgatcggggg ggcgtcgggg gaggcgccgg actcgaagac
      301 gatctcgtcg ttgcgggcat ccacgatgac cgtctgcccc gcctggaact ccttccacag
      361 gagcttctcc gagagcgggt cctcgaccat ccgctggatc gtgcggcgca gcggccgggc
      421 ccccagcgcg gggtcgtagc ccttctcggc gaggagctcc ttcgcggcct ccgtgaggac
      481 gatctcgacg tccttcgact tcagctgctc gcggacgcgc ttcgtcatga ggtcgacgat
      541 gtccttcacg tgctcgcggg agagcgagtg gaagacgatg acctcgtcga tccggttcag
      601 gaactcgggc cggaaggagc gcttgagctc ctcgttgacc ttgtccttca tcttctcgta
      661 gctcacgccc tcctcggcgt cctgccgagc gaacccgatc gaggccttcg cgaggtcccg
      721 ggtcccgagg ttcgaggtca tgatgatcac ggtgttcttg aagtccaccg tgtgcccctg
      781 ggcgtccgtg agccgtccgt cctcgaggat ctgcagcagg gtgttgaaca cgtcggggtg
      841 cgccttctcg atctcgtcga acagcacgac gctgaacggc ttgcgccgca ccgcctcggt
      901 gagctggccg ccctcctcgt accccacgta gccggggggc gagccgatca ggcgcgagac
      961 cgtgtgcttc tccatgtact cggacatgtc gagctggatc agcgagtcct cgtcgccgaa
     1021 caggaactcc gcgagggtct tcgacagctc cgtcatcccg acgccggagg gcccgaggaa
     1081 gatgaacgac ccgctcggcc gcttcgggtc cttcaggccg gcccgcgtgc ggcggatcgc
     1141 cttcgacacg gccgtcacgg cctcggactg gttgacgagg cgcttgtgca gctcgtcctc
     1201 catccgcagg agcttctggg tctcctcctc ggtgagcttg tacacgggga tgccggtcca
     1261 catggcgagg acctcggcga tctcctcctc gtcgacctcg gagaggatgt cgagctcccc
     1321 ggacttccag tccttctccc ggcgggtccg ctcctcgatg aggttccgct cctcgtcccg
     1381 caggttggcg gcggcctcga agtcctggcc gtcgatcgcc gactccttgc gcatccggac
     1441 gtcggcgatc ttctcgtcga cctcgcggac gtccggcggc gccgtcatcc ggcggatgcg
     1501 gatccgcgag cccgcctcgt cgatcaggtc gatcgccttg tcggggagga agcggtcgga
     1561 gatgtagcgg tcggagaggt tcgccgctgc caccagggcg tcgtcggtga acgagacgcg
     1621 gtggtgggcc tcgtagcggt cccgcaggcc cttgaggatc tcgatcgtgt gcgtgaccga
     1681 cggctcgttc acgtcgatcg gctggaaccg gcgctcgagg gccgcgtcct tctcgaggtg
     1741 cttgcggtac tcgtcacgcg tggtcgcgcc gatcgtctgg agctcgccgc gcgcgagcat
     1801 cggcttgagg atcgaggccg cgtcgatcgc gccctcggcg gcgccggcgc cgacgagcgt
     1861 gtggagctcg tcgatgaaca ggatgatgtc gccgcgggtc ttgatctcct tcaggacctt
     1921 cttcaggcgc tcctcgaagt caccgcggta gcgggagccg gcgacgaggg cgccgaggtc
     1981 gagcgtgtag atctgcttgc cgttcagcgt ctcggggacc tcgcccttga cgatgtcctg
     2041 cgcgagcccc tcgacgatcg cggtcttgcc gacgccgggc tcgccgatca gcaccgggtt
     2101 gttcttcgtc cggcgagaga gcacctgcat cacgcgctcg atctccttcg cgcggccgat
     2161 caccgggtcg agcttgccgt cgcgggcgag ctgcgtgagg ttgcggccga actggtcgag
     2221 gaccatcgag ccctgcggcg cctgctcggc ggcgccgggc tccttgccgc ccgtgtagcc
     2281 cgacaggagc tggatcacct gctggcggac gcggttgagg tcggcgccga gcttctggag
     2341 gacctgcgcg gcgacgccct cgccctcgcg gatcaggccg agcaggatgt gctcggtgcc
     2401 gatgtagttg tggccgagct ggagggcctc gcgcagagag agctcgagga ccttcttcgc
     2461 acggggggtg aacgggatgt ggccggccgg ggtggactgg ccctggccga tgatctcctc
     2521 gacctgctgg cggaccgcct cgagggagat gccgagggac tcgagcgcct tcgcggcgac
     2581 cccctccccc tcgtggatga ggccgaggag gatgtgctcg gtcccgatgt agttgtggtt
     2641 caggagccgc gcttcttctt gggcgaggac gacgacccgg cgggcccggt ctgtgaatcg
     2701 ctcaaacatg gccttctctt cctgaggccg gacctgccgg ccccatcggg cgtccccggg
     2761 gatcgtcgga ctccgtccta cccccgaagc gtcggtccgc tcccggcacc tccccggcgc
     2821 ccggagcgcc tcgggaccga tgataccacc gggccccgac cagacctccg agccgatccc
     2881 gcttcaaccg ctccatcgcg cctccaccgc cggccgccgg gcgaaccgct ccttccctgt
     2941 tcttcggccg cccggcgcgc gtggattagc ggcggagcga gatccgtcag ctcttcttga
     3001 cgggctccca gccttcctct tcctcgccgt cgagcttctg cttcacggtg ccgctggcct
     3061 tgcgcacgcc ctcctcgacg acgtggaggc tcttctgcgc accgctctcg acgacctccc
     3121 ggccgcgctc gacgaccgac cgcaccgtcg ggttgcccat gaagcggtcc cacgcctcgc
     3181 gcagctcctc gtagcgctcc ttgccggcct tcgtgccgag gacgtagccg acgccgaaac
     3241 cgatgatcaa ccctcgcttg aagcccatgc tttctccttt ccctccgtcc gggggcatcc
     3301 tgaggccgca ggggcctcgc tcgctccttc taccccgggt ggccggggct caccccgtcg
     3361 cgccgagcga cccccgcggt cagatccggc atcgaggtgc gatgatggat gcgtgagcga
     3421 cgcaccgaag accttcggcc ccaacgcctg gctcgtcgac gagatgtacc gacgctaccg
     3481 ggaggacccg gcgtccgtcg acgaggcgtg ggcggagttc ttcgaggggt accggccgcg
     3541 cggggccgct ccccccgagc cgcagcccgc cccgacccgg acccaggcac ccacggcacc
     3601 ggcacccgac gccgcgc
//
LOCUS       DATLGG010000051         6209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_191873, whole genome shotgun sequence.
ACCESSION   DATLGG010000051 DATLGG010000000
VERSION     DATLGG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191873"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VM638_01290"
     CDS             complement(<1..941)
                     /locus_tag="VM638_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11097.1"
                     /translation="MVSSIRSFLLVVAAITTSLTPLPGAAAGAVVPAEEVVAFHRPSA
                     GSRDIFLVTPDGLPVGNLTASPSTRDVDPSWAPSGHRIAFARRRPGSVHEIHTIAPTG
                     ESLRRLTASGFDDREPAWSPSGERIAFVRSRPARGWSRLMTMSSAGSTVASLTSARRG
                     RFDASPSWSPDGTALVFARSDGGFPSLWVVQADGSGLRRLTSEGCSDANPSWGAAGIV
                     FDRTCGGGAADLWIVDAAGGSPLNLTSTPGVAELDPDWSPGGEGIAHLTQTPGSGGVD
                     LAVRMPWTSPGTVVAAGGASEVGPDWHRLGRVGGRPCT"
     gene            complement(993..2195)
                     /locus_tag="VM638_01295"
     CDS             complement(993..2195)
                     /locus_tag="VM638_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HVM11098.1"
                     /translation="MSPRLSPRDLLAPIEARQGEFVEALRQLVSIDSGTYTPEGVNRI
                     ADLCEARFVAGGWRVERHPNEPPPGKPQLGDVVIGRLEGSGGPRILLIGHMDTVFDAG
                     TAEARPFRVEGERAFGPGVSDMKGGLLLGFFAVEALQEAGIEGFGSITYICNPDEEIG
                     SPFSRRFITEAAGEADVALVLEAARANGDIVSARKGVTDARISIRGRAAHAGVEPEKG
                     RSAVLEAIHKALALQAMNERWPDVTLNVGVIGGGTRPNVVPEHCSIEVDLRAATPATL
                     AEAEEELRRIAGTHTVPDVEVDVVVRSWHLPLEPSERSAQLAGVVRQVAGALGIPPPA
                     DVGSGGASDGNTTGAAGLPTLDGLGPIGGGWHGPSEWMDLASVPARCALLAGVLAALA
                     EGRSGRSA"
     gene            complement(2201..2434)
                     /locus_tag="VM638_01300"
     CDS             complement(2201..2434)
                     /locus_tag="VM638_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11099.1"
                     /translation="MAMFGVARAEELPGVMEGEVRRINLGEFLVVELTDEAARGFPGD
                     VFGSEEEAQEAAAREAGVVANQPGEVDPPGQQA"
     gene            2567..3250
                     /gene="sigH"
                     /locus_tag="VM638_01305"
     CDS             2567..3250
                     /gene="sigH"
                     /locus_tag="VM638_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HVM11100.1"
                     /translation="MAAKVRIKVPADLTDLSDEELVARYQAGEAAAVEILLNRYRHLA
                     HIKARSYFLAGASRDDVVQEGMIGLYKAIRDYREGRRAAFRTFADVCITRQIVSAIKT
                     ATRHKHDPLNSGLSLSKPLPQDDQPDRVLGDVLSGEDVADPADLVIQNEEILGVRRAF
                     AEVLSDFEAEVLRMYIEGKSYQDIARHLRRGVKAIDNALQRIKRKLEMSLRGDAELVG
                     TATHAGSGH"
     gene            complement(3266..3337)
                     /locus_tag="VM638_01310"
     tRNA            complement(3266..3337)
                     /locus_tag="VM638_01310"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3303..3305),aa:Val,seq:cac)
     gene            3564..4361
                     /locus_tag="VM638_01315"
     CDS             3564..4361
                     /locus_tag="VM638_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HVM11101.1"
                     /translation="MERKLAQRLVVEFVGTLALVFIGVGAIISTQAGGALITVALAHG
                     LAIAVMVSATGHISGGHFNPAVTIGAWVTQRIRLTDALTYVVAQLLGGAAGAGLIRLA
                     VPRDIWSAVNLGTPQATEVIGSGQAVLIEAILAFFLVWVIFGTVMDPDGSFHKIAGLG
                     IGLTIAMGIMMGGPFTGAAMNPARAFGPALVGNFWTAHWIWWVGPIAGGLVAALLYDT
                     VMLRTRSLPPPETDTPPPAQPEYGGPGEPEAEAVLPSSWSDEADEER"
     gene            4384..5157
                     /locus_tag="VM638_01320"
     CDS             4384..5157
                     /locus_tag="VM638_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVM11102.1"
                     /translation="MAIRELASIEEMRAAAELIDGIWGETRIVTPELLRAIATHGGLV
                     VGAYRGKALVGAQMAFLGMSADELVLHSHVTGVAPGERGTGIGRALKIAQRDWCLARG
                     IATVTWTFDPMIGRNAAFNLRSLGAEAVAFLPDFYGPMNDAFNAGDRTDRLEVRWELS
                     SERVEAALSGRASAPTHDGRVLLADHDGSPRPEPLGDADEPVVRVQIPGDYLALRARD
                     PELARRWRTAAGEAFGTAMNLGFRAVWFDPPATYVLERR"
     gene            5154..>6209
                     /gene="menC"
                     /locus_tag="VM638_01325"
     CDS             5154..>6209
                     /gene="menC"
                     /locus_tag="VM638_01325"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HVM11103.1"
                     /translation="MKPEAIELLRVRLPLRRPFTTSFGTMREKEAILVRALGPEGEGW
                     GECVAAQEPRYSAEWNDGAWLVLRDHLAPAALAGHASGVRGHPMARTALDVALLDLRL
                     RAEGRSLAEELGAIRDRVPSGVSLGLEGSLDDLLAEVEQAVGARYRRVKLKIEPGRDL
                     DVVRTVRETFPDLPLSVDSNAAYTPADLDHLTRLDAFGLAYIEQPLPEDDLLGHASLQ
                     ERIQTPVCLDESITSPTRAMEAIRADACRIINVKVGRVGGLDPSRAIHDIAVAEGVPL
                     WCGGMLETGIGRAVNLAVAAMPGFTLPGDTSASERYFPQDLTEPFVLDPDGTMPVPRG
                     PGIGVDPDPERLAEATVD"
BASE COUNT          904 a         2255 c         2224 g          826 t
ORIGIN      
        1 gtgcaggggc ggcctccgac gcgtccgagc cgatgccagt ccggcccgac ctccgaggcc
       61 ccaccggcag ccacgacggt cccgggcgag gtccatggca tccgcacggc caggtcgacg
      121 ccgccggatc cgggtgtctg cgtgaggtgg gcgatgccct cgcctccggg cgaccagtcg
      181 gggtccagct ccgcgacgcc cggggtggag gtcaggttga gcggggaacc accggcggcg
      241 tccacgatcc acaggtccgc tgccccgccg ccgcaggtgc ggtcgaacac gatcccggcg
      301 gccccccacg acgggttcgc gtcgctgcag ccttcgctcg tgaggcggcg gagcccggag
      361 ccgtcggcct ggaccaccca caggctcggg aacccgccgt ccgagcgagc gaagacgagg
      421 gcggtcccat ccggggacca cgacggcgac gcatcgaacc gaccgcgccg cgccgaggtg
      481 agcgacgcga cggtcgagcc cgccgaggac atcgtcatca ggcgcgacca cccgcgagca
      541 ggacgtgagc ggacgaaggc gatccgctcg cccgacggcg accaggcggg ttcccgatcg
      601 tcgaacccgc tcgccgtcag gcgacgcagg ctctcaccgg tgggagcgat cgtgtggatc
      661 tcgtggacgc tcccgggcct gcggcgagcg aaggcgatcc ggtggccgga tggcgcccag
      721 ctcgggtcga cgtccctcgt ggaggggctg gcggtcaggt tcccgaccgg aaggccgtcg
      781 ggagtcacga ggaagatgtc gcgcgacccc gccgacgggc ggtggaaggc cacgacctcc
      841 tcggcgggaa cgaccgcgcc agcggcggcg cctgggagcg gcgtcagcga ggtcgtgatc
      901 gcggcgacga cgaggaggaa ggatcggatc gaggacacca tgctttctgc cagaaggcgg
      961 acgtcccaca gtaggaaggg gtcgcagagg cgtcaagcag agcggccgga gcgcccttcg
     1021 gcgagcgccg ccagaacgcc ggccaggagc gcgcatcgcg cggggaccga cgcgaggtcc
     1081 atccactcgg acgggccgtg ccacccgccg ccgatgggcc cgagcccgtc gagcgtgggg
     1141 agcccggccg cgccggtcgt gttcccgtcc gaggcgccgc cggagccgac gtcggccggt
     1201 ggcgggatcc cgagggcccc ggccacctgg cggaccaccc ccgcgagctg cgcggaccgc
     1261 tccgagggct cgaggggcag gtgccagctc cggaccacga cgtcgacctc gacgtcgggg
     1321 acggtgtggg tcccggcgat ccgtcgcagc tcctcctcgg cctcggccag ggtcgccggg
     1381 gtcgccgccc gaaggtcgac ctcgatcgag cagtgctcgg ggacgacgtt cgggcgggtg
     1441 ccgcctccga tcacccccac gttgagggtc acgtccggcc agcgctcgtt catcgcctgg
     1501 agggcgagcg ccttgtggat cgcctcgagg accgcgctcc tgcccttctc cggctcgacg
     1561 ccggcatggg cggcgcggcc ccggatcgag atcctcgcgt cggtgacgcc cttgcgggcg
     1621 gagacgatgt caccgttcgc cctcgcggcc tccagcacga gcgcgacgtc agcctccccc
     1681 gcggcctccg tgatgaagcg gcgcgagaac ggcgaaccga tctcctcgtc cgggttgcag
     1741 atgtaggtga tcgagccgaa cccctcgatc ccggcttcct ggagggcttc gacggcgaag
     1801 aagccgagga ggaggccgcc cttcatgtcg gagacgccgg ggccgaaggc tcgctcaccc
     1861 tccacgcgga acggcctcgc ctccgcggtg cccgcgtcga agacggtgtc catgtggccg
     1921 atcagcagga tccgcggacc accggagccc tccagacgcc cgatcaccac gtcacccagc
     1981 tgcggcttgc ccgggggtgg ctcgttcgga tgccgctcga cccgccagcc gccggcgacg
     2041 aaccgcgcct cgcacaggtc cgcgatccgg ttcaccccct cgggcgtgta ggtgcccgag
     2101 tcgatgctga ccagctgtct gagggcttcg acgaactcgc cctgccgtgc ctcgatcgga
     2161 gcgagcaggt cccgcggcga gaggcggggc gacaacggat tcaggcctgc tggcccggcg
     2221 ggtcgacctc gcccggttgg ttggcgacca cgccggcctc ccgggccgcc gcctcctgcg
     2281 cttcctcctc gcttccgaag acgtcaccgg ggaagccgcg ggctgcctcg tcggtgagct
     2341 ccacgaccag gaactccccc aggttgatcc gacggacctc tccctccatg acccccggga
     2401 gctcctcggc acgcgccacc ccgaacatcg ccatcgtcca cctccatcgg atcgaaccgg
     2461 gccgggctcc ggaatcttcg cataccgaga aggattccgg ccgcggatgg cgaagtatga
     2521 gtccaagggt cggggagcac ccggcctcgg acgaaaagga gttcgcgtgg cggcgaaggt
     2581 caggatcaag gtgccggccg atctcacgga cctctccgac gaggagctcg tcgcccggta
     2641 ccaggccggg gaagccgctg ccgtcgagat cctcctgaac cgctaccggc acctcgccca
     2701 catcaaggcc cgttcctact tcctcgcggg cgcgagccgc gacgacgtcg tccaggaggg
     2761 catgatcggc ctctacaagg cgatccgcga ctaccgcgag ggccgccgcg ccgccttccg
     2821 gaccttcgcc gacgtctgca tcacccgtca gatcgtgagc gcgatcaaga ccgccacccg
     2881 gcacaagcac gacccgctga acagcgggct ctccctctcc aagcccctcc cccaggacga
     2941 ccagccggac cgcgtgctcg gcgacgtgct gagcggtgag gacgtcgccg accccgccga
     3001 cctcgtgatc cagaacgagg agatcctggg ggtccggcgc gcgttcgccg aggtcctctc
     3061 ggacttcgag gccgaggtcc tgcggatgta catcgagggc aagtcctacc aggacatcgc
     3121 gcggcatctc cgccgcggcg tgaaggcgat cgacaacgcc ctccagcgga tcaagcggaa
     3181 gctggagatg agcctgcggg gcgacgccga gctcgtcggc acggccacgc acgccggcag
     3241 cggacactga ccatcacccg gtcggtgggc gcggcaggat ttgaacctac gacctctcgc
     3301 gtgtgaggcg agcgctctcc cgctgagcta cgcgccctgg ggctgaccag cctacaggaa
     3361 ccgggcctcg ccgtggaagc ccgcccggaa aaggggacgg cggggtgtgc ccgcgtcacc
     3421 ccgccgtcag gcccgagccc ggaggctcgg acgtctgaac acagtgtctc accgccgacc
     3481 cgattttgca atggggggcc cgcgttcgtc aggataccgg tgcccgccgc ccgctcgagg
     3541 cccgggcgca ggaaggggga aggatggaac ggaagctggc acagcggctc gtcgtcgagt
     3601 tcgtcggcac cttggcgctg gtcttcatcg gggtcggcgc gatcatcagc acccaggccg
     3661 gaggggccct catcacggtg gccctcgcgc atgggctcgc gatcgcggtc atggtctccg
     3721 cgaccgggca catctcgggc ggccacttca acccggccgt gacgatcggg gcctgggtga
     3781 cccagcgcat ccggctgacg gacgcgctga cctacgtcgt ggcgcagctc ctcggtggcg
     3841 ccgccggagc gggactgatc cggctggcgg tccctcgcga catctggtcg gcggtgaacc
     3901 tcgggacgcc gcaggcgacc gaggtgatcg ggtcggggca ggccgtgctg atcgaggcga
     3961 tcctggcgtt cttcctcgtc tgggtgatct tcggaacggt catggacccg gacggctcct
     4021 tccacaagat cgccggactc gggatcggcc tgacgatcgc gatggggatc atgatgggcg
     4081 gccccttcac gggcgcggcc atgaacccgg cgcgcgcctt cggcccggcg ctcgtcggca
     4141 acttctggac ggcgcactgg atctggtggg tcggcccgat cgccggcgga ctcgtcgccg
     4201 cgctcctgta cgacaccgtc atgctgagga cgcgctcgct gccgccgccc gagaccgaca
     4261 cgccacctcc cgcccagccg gagtacggcg gcccgggcga gcccgaggcc gaggcggtcc
     4321 tcccgtcgag ctggagcgac gaggcggacg aggagcgata aggaggttcc gacgcgcgaa
     4381 ggggtggcga tccgcgagct cgcctcgatc gaggagatgc gcgcggctgc cgagctcatc
     4441 gacgggatct ggggcgagac ccggatcgtc accccggagc tgctgcgggc catcgcgacc
     4501 cacggcgggc tggtcgttgg cgcctaccgg gggaaggcgc tcgtcggcgc ccagatggcc
     4561 ttcctcggca tgtcggccga tgagctggtg ctgcactcgc acgtgacggg cgtggcgcca
     4621 ggcgagcgcg ggaccgggat cgggcgcgcg ctcaagatcg cgcagcgcga ctggtgcctc
     4681 gcccgcggga tcgcgaccgt cacctggacc ttcgacccga tgatcgggcg gaacgccgcc
     4741 ttcaacctgc gatccctcgg cgcggaggcc gtcgcgttcc tgccggactt ctacggcccg
     4801 atgaacgacg ccttcaacgc cggcgaccga accgaccgcc tggaggtgcg atgggagctg
     4861 tcctcggagc gggtcgaggc cgccctttcg ggacgcgcct cggcccccac ccacgacggc
     4921 cgggtgctgc tggcggacca cgacggaagc ccgcgtcctg aaccgctcgg cgacgcggac
     4981 gagcccgtgg tgcgcgtgca gatccccggc gactacctcg cgttgcgggc gcgcgacccc
     5041 gagctcgcac ggcgatggag gaccgccgcg ggtgaggcgt tcgggacggc gatgaacctc
     5101 ggtttccgcg ccgtctggtt cgacccgccg gcgacctacg tgctggagcg ccgctgaagc
     5161 cggaagcgat cgagctgctg cgcgtccggc tcccgctccg gcgccccttc acgacgtcgt
     5221 tcggcacgat gcgcgagaag gaggcgatcc tcgtgcgagc gctcggaccc gagggcgagg
     5281 ggtggggcga gtgcgtcgcg gcgcaggagc cgcggtactc ggcggagtgg aacgatggcg
     5341 cgtggctcgt cctgcgcgat cacctcgccc cggcggcgct cgccggtcac gcctcgggcg
     5401 tgcggggtca tccgatggca cggacggcgc tcgatgtcgc gctcctcgac cttcgcctcc
     5461 gggcggaggg tcgcagcctc gcggaggagc tcggcgcgat ccgcgaccgc gtcccctccg
     5521 gcgtgagcct cggcctcgag ggatccctcg acgacctcct cgccgaggtg gagcaggccg
     5581 tgggagcccg ctaccggagg gtgaagctga agatcgagcc cggccgcgac ctcgacgtcg
     5641 tgcgcacggt gcgggagacg ttcccggacc ttcccctgag cgtcgactcg aacgccgcct
     5701 acactccggc ggacctcgac cacctgacac gcctcgatgc cttcgggctc gcgtacatcg
     5761 agcaacccct gccggaggac gacctgctcg ggcacgcctc tctccaggag cggatccaga
     5821 caccggtctg cctcgacgaa tcgatcacct cccccacccg cgcgatggag gcgatccggg
     5881 ccgatgcctg ccggatcatc aacgtgaagg ttgggcgcgt cggcggcctc gacccctcac
     5941 gcgcgatcca cgacatcgcg gtcgcagagg gcgtcccgct ctggtgcggc ggcatgctcg
     6001 agacggggat cggccgagcc gtcaacctcg ccgtcgccgc gatgcccggc ttcacgctcc
     6061 ccggagacac gagcgcgtcg gagcggtact tcccccagga tctcaccgag ccgttcgtcc
     6121 tcgaccccga cgggacgatg ccggtgccgc gcggaccggg gatcggcgtc gacccggatc
     6181 cggagcgcct cgccgaggcg accgtcgac
//
LOCUS       DATLGG010000052        11402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_50747, whole genome shotgun sequence.
ACCESSION   DATLGG010000052 DATLGG010000000
VERSION     DATLGG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50747"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VM638_01330"
     CDS             <1..1498
                     /locus_tag="VM638_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVM11104.1"
                     /translation="SGGINEELFPGFAELAEGSTWFRHYTVAEAHTYFSVPTILSGRL
                     ITDRTKQALAVDHPNTLFTMLRDTHRMNVVEPVTNLCPEDLCAGTAPSAASPQGSPVA
                     ALLRDAAGVWTEISLPIHVTRDVTAQFAEAADPDAAVDREAGRGTSSPFDGFLASIRR
                     ERDPTLHYFHTLLPHLPWRRFPSGATYETLRGARDDQPLVREARWRWVDEPWAVQLAR
                     QRHILQARYVDALVAELTAHLKREGLWEDALVIVTSDHGIPLQPGRSRAPTQENVHEI
                     YWVPMFLKAPGQQEGAVDDRNVTATDLLPTIADALGTRVPWQVEGTSMLDPSTDRGSE
                     KVIRQLYGDERRPDTFRIGLEEAERRLRAEAFRADVRSADPDILPFLVGPRPDLIGRS
                     IEDLDMGTASGRTATLDLPTRTRAGPEDPLPAFVWGTLEDSRAGEEVLVAAAVNGRIA
                     GVSTTWVQDGVPGRFGVIAPETFVRRGENALALFVLEGDELRPIEVRR"
     gene            1528..2538
                     /locus_tag="VM638_01335"
     CDS             1528..2538
                     /locus_tag="VM638_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVM11105.1"
                     /translation="MDAQTILVTGGAGFLGSHFVRAWLAETAGHLVNLDLLTYAGNAE
                     RLDDLAGDERYRFVHGDVADRDLVRATLRDSAPELVVHFAAESHVTRSELDPGRFHRT
                     NVDGTLVMLEEAAAANVRRFVHISTDEVYGPILDGAFREEDKEPGEGRATSPYARSKA
                     LADDVATGFDADMGVVVVRPTNAFGPWQFPEKAFPRWITRAVRGEPLLVWGDGLYVRQ
                     WLYAEDLSHALALILGAAAPEPVYNVGPRHEPEITNLDLARWLVEHLGLPEDRLRLTE
                     YDRPDHDRRYAVDPARIEGLGWKPSDPWERFAETVAWYRANDAWWRPLVAEAESIYRD
                     AR"
     gene            2538..3062
                     /locus_tag="VM638_01340"
     CDS             2538..3062
                     /locus_tag="VM638_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HVM11106.1"
                     /translation="MEELAIPGVVLARPARHEDDRGWFMEIMREEAFRDVFVQANHSR
                     SKQGVLRGLHYHREQADAWYVLSGVARVGLADLRRPHEGIATLTVELRGDEPAVLYLP
                     PGVAHGFYAVTDLDLIYFVTRYYDSSDEHSVAWDDPALRVPWGVEEPILSERDRTAPP
                     LDPAEVERVLRAGG"
     gene            3127..4425
                     /locus_tag="VM638_01345"
     CDS             3127..4425
                     /locus_tag="VM638_01345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVM11107.1"
                     /translation="MKIAVIGLGYVGLTGAVSLAEAGHRVTGVERDPSRLDRLRGGTT
                     PFYEPGLQDLLARTTTSGALAFVGSLGDLSDPPDAVLVTVGSPQLPSGGTDLRDLATA
                     MGEVLALDPAPQLVVVKSTVPPGTSATLLADNPGLAGRYAFSPEFLSQGSALEGWRRP
                     SRIVVGLEDEALLPLVRDLYRGVEGPWVVTNPTNAEMIKYASNAFLATKISFINEIAN
                     LCDDVGATVDEVVEGLGLDPRVGRSFLGAGIGYGGSCFPKDVQALTHLSSLRGRSMPL
                     LQSVVAVNNAQRLRVVRAVRDHLRPGDEVAVLGLSFKPNTDDLREAPSLTVVPELLAQ
                     DLRVRAWDPVVGDERIREAFAGVEPATGPEQAAEDAAAVLVLTEWPQIVEADWEIVAK
                     GMREPRLVLDGRNCLDPARLARLGLRYWGIGRGLRDPEER"
     gene            4422..5321
                     /locus_tag="VM638_01350"
     CDS             4422..5321
                     /locus_tag="VM638_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVM11108.1"
                     /translation="MSDRVDVTIVLPVYNEVGHLRQEVQRIKTSMDASPYTYEILAVD
                     DASTDGSGDALAQIDGIRLIRFNQNRGSGTARKVGTRAARGDVVVWTDCDMTYPNDEI
                     PRLVKELEGFDQVVGARTSEQGTKKALRVPAKWMIRKLASYLVETRIPDLNSGFRAFR
                     RDVAMQFVHQLPPGFSCVTTLTLSFLSNGYSVNYIPIDYHPRAGESKFHWWRDTKRYI
                     TQVVRMILSYNPLKVFLPLGFTLLTVGIVKLIYDWSVRDFSLASNTLLILFAAFQVFA
                     IGLLADLVVRMNRPRDEVDPASG"
     gene            complement(5322..6389)
                     /locus_tag="VM638_01355"
     CDS             complement(5322..6389)
                     /locus_tag="VM638_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HVM11109.1"
                     /translation="MRVFVAAWVGSTNAGDELVFEALRRKLAARGARVAVVSVDPDRT
                     REAHGVEAVPSRNVAAVHAAIRRSDRLVLGGGGLLQDETSHLNLPYHLWRPWTARRAR
                     IPFAGVGLGAGRLTSSMGRSLVRRALAGSRGVAVRDGESAALLTSLGVRGVVTAADLV
                     LSLEAPAVEPADRLVVCLRSWPSRGRLLPMSLRWRQDLAEERWLDAVAAGLDEAAAAT
                     GLDVRFVAFHRGRDDVLHRRVGERMRQTVSFAEPEPDALPREIARGRAVVSMRYHGGI
                     LAAMAGRPLVLVGGSAKLGSLAASLGRGAVLLGRDAAAIRGVTRHLDAVLPGATDLPE
                     AVERLRQGERGNDEVLDRLLA"
     gene            complement(6386..7615)
                     /locus_tag="VM638_01360"
     CDS             complement(6386..7615)
                     /locus_tag="VM638_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVM11110.1"
                     /translation="MRILLVTSNFPRWDGDSTTPFVLHLAQGIQDLGARVDVLAPHAP
                     GAARHEILGGVRVERFRYLLPESQQTVCYQGGALVNLRDRPTNALKLPALVAAEWAAL
                     ASRLWRRRYDVVNSHWILPQGFVAALAARPLGIAHVTTVHGGDIFALRGAAMRAFKRS
                     ALRQADAVTVNSSVTREAVLAVTDRIRHLETIPMGVRAEPAPAATAVASVRERHRSDG
                     GPLLAFAGRLVEEKGVEDLVRAVRLLRDRLPGTSLIVIGEGQHRARFERVATEEGVAD
                     RVRFAGWLPPGEVRAHLAAADIVVAPSRQAPDGWIEAQGLTVVEAMTAGTPVIASRSG
                     GVVDAVRDGETGLLVPERSPEAIAAAVERIVRDPTLAGRLARAGADLAQARFSQRATA
                     EAFVRLFERVRAARGSR"
     gene            complement(7630..9033)
                     /locus_tag="VM638_01365"
     CDS             complement(7630..9033)
                     /locus_tag="VM638_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11111.1"
                     /translation="MRSRSSSAAQVPGSRIPSLIEDLEVRGSTIRVRADRAFTERFLR
                     EDFVVEYERDIDLRTLASSTLLLPFVLNVAPILWAAGWEARVEEMDEELASVLGPIRE
                     TYRRLYPRVRWDGQLHAATVRRGPDAPPVAGAAGTAVLFSGGVDSTATSLLLDEPQLL
                     ITIWGNEIALDNQEGWGSARGYTEAYARRHGHRPGFIRSNFRSFLDVSRLNGAFPGIT
                     NWWLDVQHGIGLVGLAAPLMAARGANRILFSSGRTEEQITALGAGPDLEGRLRWSGRR
                     AEHALGETRRHDKLRSVVERVRSGEAREITLRVCFMYPYGGGGNCGACEKCLRTMAGL
                     LVEGEDPRAYGFPGSPRKVSEAIRRRFRTLRMRFGEAPGHYWEELAQRAAERLGIEGA
                     DLRAAGEPIGGFLRWVSTYDFAAYARRWARVGAARAWAIATIRRSPRATRIAMRLMAR
                     ARRRRLRSPGAPGEGTG"
     gene            9128..10168
                     /locus_tag="VM638_01370"
     CDS             9128..10168
                     /locus_tag="VM638_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11112.1"
                     /translation="MSREQGPETPTVEEPDRAEGGEPGGGGRLTRALLAFGNRLIAWV
                     RRRHANRSPRTERILLVLAVLLFIVVTWLGFRTMPDVGRPVRWGVVALAAAVGVPLST
                     LLNAFEYRLSGKILGHRIALLEAIRVSVLASAANRLPIPGAALVRIGALRRLGSGFGK
                     ATTATAAVGIVWTGTSFATAGVLQCVAARWILGLTFLGGGLAGIGLGYALMVWRARPA
                     RAVRLTAELLLIELAAVALPTVKMLVVLQAIGFDVTVAQSAALAVSIVVAHATGFFPG
                     GLGLREVIAGAMSPLVGLPVSVGLLGTAVTRAFSLVMSLLIAAIVLIPYLRGKKASAA
                     AEAVGPPVPHDD"
     gene            complement(10172..>11402)
                     /locus_tag="VM638_01375"
     CDS             complement(10172..>11402)
                     /locus_tag="VM638_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11113.1"
                     /translation="AEYEGISLEQVPRWVLELPFLLNVAPVVWLSGRRFTVPELDEEL
                     ASSLGRVRAAFQRLYPHLSWSGEIRAERLVGHDLEGAPHDPVTILFTAGIDSTHSSVR
                     NADRRQLLVSVWGTGIALDNTAGWAQVRRMCLAHAERTGNEAAFVRSNVRSFLEPARI
                     MALSPGIPHWWTRVAFGLGYTGLAAPAMAAKGSRRLVVSASHTHTSDEPYGSHPDTDG
                     QLRWGGAEVSHEGFELTRHDKVREIVAHVRSGGVRPMLRPCYSYKYGSGENCGRCEKC
                     LRTSTALLVEGEDPRGYGIPVDPAKLSRVIDGQMRRFTLILAQANRVFHWTDMQRHAR
                     RVLQEGMPRKLGDGTDVRPFLRWLVSRDFPRYARRWRIIGAMRTRISRVVARFPAIER
                     GLRRLQARLRDRRTARV"
BASE COUNT         1590 a         4289 c         4004 g         1519 t
ORIGIN      
        1 gtccgggggc atcaacgagg agctgttccc cggcttcgcc gagctcgccg aggggtccac
       61 ctggttccgt cactacacgg tcgccgaggc gcacacctac ttctccgtcc cgacgatcct
      121 gtccggacga ctcatcaccg accgcacgaa gcaggccctc gcggtcgacc atccgaacac
      181 cctgttcacg atgctccgcg acacccaccg catgaacgtc gtcgaaccgg tgacgaacct
      241 ctgccccgag gacctctgcg cggggaccgc gccttcggcg gcctcgcccc agggctcccc
      301 ggtggcggcc ctcctgcgcg acgccgccgg ggtgtggacc gagatctcgc tcccgatcca
      361 cgtcacgcgc gacgtgacgg ctcagttcgc cgaagccgcc gaccccgacg cggcggtcga
      421 ccgcgaggcg ggccggggga cctcctcccc cttcgacggg ttcctcgcat cgatccggcg
      481 ggagcgtgac ccgaccctgc actacttcca cacgctcctg ccccacctcc cctggcgccg
      541 cttcccctcc ggcgccacgt acgagacctt gcgcggcgcg cgcgacgatc agcccctcgt
      601 ccgcgaggcg cggtggcgct gggtcgacga gccgtgggcg gtccagctcg cccgacagcg
      661 ccacatcctg caggcccgct acgtcgatgc gctcgtggcc gagctgacgg cgcatctgaa
      721 gcgggagggc ctgtgggagg acgcgctcgt catcgtcacc tccgaccacg ggatccccct
      781 ccagccgggc cgttcccgcg cacccacgca ggagaacgtc cacgagatct actgggtgcc
      841 gatgttcctc aaggcgcccg gccagcagga gggagccgtc gacgaccgca acgtcacggc
      901 gaccgacctc ctgccgacga tcgccgacgc cctcggaacc cgcgtgccgt ggcaggtgga
      961 aggcacctcg atgctcgacc cctccaccga tcgaggctcg gagaaggtga tccgacagct
     1021 ctacggcgac gagcggcgcc ccgacacctt ccgcatcgga ctcgaggagg cggagcggcg
     1081 gctccgagcg gaggcgttcc gggcggacgt gcggtcggcc gacccggaca tcctcccctt
     1141 cctcgtgggg ccacgccccg acctgatcgg ccgctccatc gaggatctcg atatggggac
     1201 ggcgtcgggc cggacggcga cgctggacct cccgacgcgg acgcgggccg gaccggagga
     1261 cccgctgccg gccttcgtct gggggacgct cgaggactcg cgggcgggcg aggaggtgct
     1321 cgtcgccgcc gcggtgaacg gccgcatcgc gggcgtctcg acgacctggg tgcaggacgg
     1381 ggttcccggt cgcttcggcg tcatcgcgcc ggagacgttc gtccgccggg gcgagaacgc
     1441 gctcgcgctc ttcgtcctgg agggcgacga gctccggccg atcgaggtgc ggcgctagcg
     1501 gcatggcctg tcggggataa tgcgccggtg gacgcgcaga cgatcctcgt caccggcggc
     1561 gccggcttcc tcggaagcca cttcgtgcgg gcctggctcg ccgagacggc gggacacctg
     1621 gtcaacctcg acctcctgac ctacgcgggc aacgcggagc ggctcgacga tctggccggc
     1681 gacgagcgct accggttcgt gcacggggac gtcgccgacc gcgacctcgt ccgcgcgacg
     1741 ctgcgcgact cggctccgga gctcgtcgtg cacttcgcgg cggagtcgca cgtcacccgc
     1801 agcgagctgg accccggacg cttccaccgg acgaacgtcg acggcaccct cgtgatgctc
     1861 gaggaggcgg cggcggcgaa cgtgcggcgg ttcgtgcaca tctccaccga cgaggtctac
     1921 ggcccgatcc tcgacggcgc cttccgggaa gaggacaagg agccgggcga aggtcgggcc
     1981 acgagcccgt acgcgaggag caaggcgctc gccgacgacg tcgcgacggg cttcgacgcc
     2041 gacatggggg tcgtcgtcgt ccgcccgacg aacgccttcg gcccctggca gttccccgag
     2101 aaggccttcc cccgatggat cacgcgagcg gtccgggggg aacccctgct cgtgtggggc
     2161 gacggcctct acgtccgcca gtggctgtac gccgaggacc tcagccatgc gctcgccctc
     2221 atcctgggcg cggccgcgcc ggagcccgtc tacaacgtcg gtccgcggca cgagccggag
     2281 atcacgaacc tcgacctcgc gcgctggctg gtcgagcacc tcgggctccc ggaggaccgc
     2341 ctgcggctga cggagtacga ccgcccggac cacgaccgcc gctacgcggt cgaccccgcc
     2401 cggatcgagg gtctcggctg gaagccgtcg gacccgtggg agcggttcgc cgagaccgtc
     2461 gcctggtatc gcgcgaacga cgcgtggtgg cgtccgctcg tggccgaagc cgagtcgatc
     2521 taccgcgacg cgcgctgatg gaggagctcg ccatccccgg ggtcgtcctc gcccgcccgg
     2581 cgcggcacga ggatgaccgc ggctggttca tggagatcat gcgcgaggag gcgttccgcg
     2641 acgtcttcgt ccaggcgaac cactcgcgtt cgaagcaggg cgtcctccgc gggctccact
     2701 accaccgcga gcaggccgac gcctggtacg tcctgtcggg cgtcgcccgt gtgggcctcg
     2761 ccgacctccg gcgcccgcac gaggggatcg cgaccctcac ggtcgagctg cgtggggacg
     2821 agcccgcggt cctgtatctc ccgcccggcg tggcgcacgg gttctacgcg gtcaccgacc
     2881 tcgacctgat ctacttcgtc acccggtact acgacagcag cgacgagcac tcggtcgcgt
     2941 gggacgatcc ggccctccgg gtcccgtggg gcgtcgagga gccgatcctg tccgagcgcg
     3001 accggaccgc gccgcccctc gaccccgcgg aggtcgagcg cgtgctccgg gccggcggtt
     3061 gagcctgtag actgcgtctc ggccgtcacc ggccgcctcc cacctgcacc cgaggagcgc
     3121 gagcgcatga agatcgccgt catcgggctg gggtacgtcg gtctcaccgg cgccgtcagc
     3181 ctcgccgagg ccggtcatcg ggtgaccggc gtcgaacgcg acccgtcgcg tctcgatcgc
     3241 ctgcgcggtg ggaccacacc gttctacgaa ccgggcctgc aggatctcct cgcccgcacc
     3301 accacctcgg gagcgctggc gttcgtcggg agcctcggcg atctgtccga cccccccgac
     3361 gcggtcctcg tgacggttgg gtcgccccag cttccgtcgg gcggaacgga cctgcgcgac
     3421 ctcgcgacgg cgatgggcga ggtcctcgcg ctcgaccccg cgccccaact cgtcgtcgtg
     3481 aagagcaccg tccccccggg gacgagcgcg acgctgctcg cggacaaccc gggcctcgcc
     3541 gggcgctacg ccttcagccc cgagttcctc agccagggct ccgccctcga gggatggcgg
     3601 cggccctcgc gcatcgtcgt cggcctcgag gacgaggcgc tgctgccgct cgtgcgcgac
     3661 ctgtaccgcg gcgtcgaggg gccctgggtc gtgacgaacc ccacgaacgc cgagatgatc
     3721 aagtacgcga gcaacgcgtt cctcgcgacc aagatcagct tcatcaacga gatcgcgaac
     3781 ctctgcgacg acgtcggcgc cacggtcgac gaggtggtcg agggactggg gctcgaccca
     3841 cgggtcggcc ggagcttcct cggcgccggc atcggctacg gcggctcctg cttcccgaag
     3901 gacgtgcagg cgctgacgca cctgtcgagc ctgcgcggcc gctcgatgcc gctcctgcag
     3961 tcggtggtcg ccgtcaacaa cgcccagcgc ctccgcgtcg tccgcgcggt ccgcgaccac
     4021 ctccgccccg gcgacgaggt cgcggtcctc ggtctcagct tcaagccgaa caccgacgat
     4081 ctccgggagg cgccgagcct caccgtcgtc cctgagctcc tcgcgcagga cctgcgcgtg
     4141 cgcgcctggg accccgtcgt cggcgacgag cggatccgcg aggccttcgc cggtgtcgag
     4201 ccggcgaccg ggccggagca ggcggccgag gatgcggcgg cggtcctcgt gctgaccgag
     4261 tggcctcaga tcgtcgaagc ggactgggag atcgtcgcga aggggatgcg ggagccacgg
     4321 ctcgtgctgg acgggcggaa ctgcctcgac cccgcgcggc tcgccaggct cggcctgcgc
     4381 tactggggca tcggccgagg gctccgggat ccggaggagc gatgagcgac cgcgtcgacg
     4441 tcacgatcgt cctccccgtc tacaacgagg tcggccacct ccgacaggag gtccagcgga
     4501 tcaagacctc gatggacgcc tcgccgtaca cgtacgagat cctcgcggtc gacgacgcct
     4561 cgaccgacgg ctcgggcgac gcgctcgcgc agatcgacgg gatccggctg atccgcttca
     4621 accagaaccg aggctccggc accgcccgca aggtcgggac gcgcgcggcg cgcggcgacg
     4681 tcgtcgtgtg gaccgactgc gacatgacct acccgaacga cgagatccct cgcctggtga
     4741 aggagctcga aggattcgac caggtcgtcg gtgcgcgcac ctccgagcag ggcacgaaga
     4801 aggccctgcg ggtcccggcg aagtggatga tccgcaagct cgcgagctac ctcgtcgaga
     4861 cgaggatccc cgacctcaac tccgggttcc gggccttccg ccgcgacgtc gcgatgcagt
     4921 tcgtccacca gctgccgccc gggttctcgt gcgtcacgac gctgacgctt tcgttcctgt
     4981 cgaacggcta ctcggtcaac tacatcccga tcgactacca cccgcgcgcg ggtgagtcga
     5041 agttccactg gtggcgcgac acgaagcgct acatcacgca ggtcgtgcgg atgatccttt
     5101 cgtacaaccc actgaaggtc ttcctcccgc tcggcttcac gctcctcacg gtcgggatcg
     5161 tgaagctgat ctacgactgg agcgtccgcg acttcagcct ggcgtcgaac acgctgctga
     5221 tcctcttcgc cgcgttccag gtcttcgcga tcgggctgct cgccgacctc gtcgtgcgga
     5281 tgaaccggcc gcgcgacgag gtggatcccg cgtccggcta gctaggcgag gagccggtcg
     5341 agcacctcgt cgttcccccg ctccccctgg cggagccgct cgaccgcctc gggcaggtcg
     5401 gtcgcgccgg ggagcaccgc gtcgaggtgg cgcgtcacgc cgcggatcgc cgcggcatcg
     5461 cggccgagga gcacggcgcc ccggccgagc gatgcggcga gggagcccag cttcgccgac
     5521 cccccgacga gcacgagcgg acgacccgcc atcgccgcga ggatcccccc gtggtaacgc
     5581 atcgagacga ccgcgcgccc gcgggcgatc tcgcgcggga gcgcatccgg ctccggctcg
     5641 gcgaacgaaa ccgtctgccg catccgctcg cccacccgac ggtgcaggac gtcgtcgcga
     5701 ccgcggtgga aggcgacgaa ccgcacatcg agacccgtcg ctgccgcggc ctcgtccagg
     5761 ccggccgcga ccgcgtcgag ccagcgctcc tcggccaggt cctgccgcca gcggagcgac
     5821 atcggcagga gccggccccg cgacggccac gaccgcaggc agacgacgag gcgatccgcc
     5881 ggctccacgg cgggggcctc gagcgagagg acgaggtccg cggcggtgac gacgccgcgc
     5941 accccgagcg aggtgagcag cgccgccgac tcgccgtcgc gcacggccac gccgcggctc
     6001 cccgcgagcg cccggcggac gagcgagcgc cccatcgagg aggtgagccg cccggcgccg
     6061 aggccgacgc cggcgaacgg gatccgggcc cgccgtgccg tccacggccg ccacaggtgg
     6121 tacggcaggt tcaggtggct cgtctcatcc tggaggaggc cgccgccacc gaggaccagc
     6181 cggtcggagc ggcggatcgc cgcgtggacc gccgcgacgt tccggctcgg caccgcctcg
     6241 acgccgtgcg cctcgcgcgt gcggtccggg tcgaccgaga cgacggccac gcgggctccg
     6301 cgggcggcca gcttgcggcg cagcgcctcg aacaccagct cgtcaccggc gttcgtcgag
     6361 ccgacccacg cggcgacgaa gaccctcatc ggctccctcg cgcggcgcgc acccgttcga
     6421 agagccgcac gaacgcctcc gccgtcgccc gttgcgagaa gcgtgcctgc gcgaggtcgg
     6481 caccggcgcg cgccagccgc cccgccagcg tcgggtcgcg gacgatccgc tcgaccgcgg
     6541 cggcgatcgc ctcgggcgag cgctccggaa ccaacaggcc cgtctcccca tcgcgcaccg
     6601 cgtcgacgac accgccggac cgactcgcga tcaccggcgt ccccgccgtc atcgcctcga
     6661 ccacggtcag gccctgcgcc tcgatccacc cgtccggcgc ctgacgcgac ggggcaacga
     6721 cgatgtcggc ggccgcgagg tgcgcccgga cctcaccggg cgggagccac ccagcgaacc
     6781 gcacccggtc ggcgacgccc tcctcggtcg ccacccgctc gaaccgcgcg cggtgctgcc
     6841 cctcgccgat cacgatgagc gacgtccccg gaagccgatc gcgcaggagg cggaccgccc
     6901 gcacgagatc ctcgacgccc ttctcctcga cgagccgtcc ggcgaaggcg aggagcgggc
     6961 ccccgtcgct gcggtgacgc tcgcgcaccg acgccacggc cgtcgcggcc ggggccggct
     7021 ccgcccgcac gcccatcggg atcgtctcga ggtggcggat acggtcggtc accgccagca
     7081 cggcctcccg cgtgaccgag ctgttcacgg tcacggcgtc cgcctgccgg agcgcggagc
     7141 gcttgaacgc ccgcatcgcc gctcctcgga gggcgaagat gtcgcctccg tgcaccgtcg
     7201 tgacgtgggc gatgcccagc gggcgggccg cgagggcggc gacgaacccc tgcgggagga
     7261 tccagtggct gttcacgacg tcgtagcgac ggcgccacag ccgcgaggcg agcgcggccc
     7321 actcggccgc cacgagcgcg gggagcttga gcgcgttcgt gggccggtcg cgcaggttca
     7381 cgagcgcacc cccctggtag caaacggtct gctgcgactc ggggaggagg taccggaagc
     7441 gctcgacccg gaccccgccc aggatctcgt gcctggcggc gcccggcgcg tgcggcgcga
     7501 ggacgtcaac gcgcgctccg aggtcctgga tcccctgcgc caggtggagg acgaacggcg
     7561 tcgtgctgtc tccatcccag cgcgggaagt tcgacgtgac gaggaggatg cgcatcgtcg
     7621 gtccctggat cagcccgtgc cctcgcccgg cgctccgggt gagcggaggc gccggcggcg
     7681 ggcgcgagcc atcaggcgca tcgcgatccg cgtcgccctc ggcgaccgac ggatcgtcgc
     7741 gatcgcccac gcgcgcgcgg cgccgacgcg cgcccagcgc cgcgcgtagg ccgcgaagtc
     7801 gtaggtcgag acccaacgga ggaacccgcc gatcggctcg ccggccgccc gcagatcggc
     7861 tccctcgatg ccgagacgct ccgccgcacg ctgcgccagc tcctcccagt agtgccccgg
     7921 cgcctcaccg aagcgcatcc gcagggtccg gaaccggcgt cggatcgcct ccgagacctt
     7981 ccgggggctg ccggggaagc cgtacgcccg cgggtcctcc ccctcgacga ggagccccgc
     8041 catcgtccgc aggcacttct cgcacgctcc gcagttcccg cccccaccgt aggggtacat
     8101 gaagcagacc cgcagggtga tctcccgcgc ctccccggag cgcacgcgct ccaccaccga
     8161 gcggagcttg tcgtggcgcc gggtctcccc gagcgcgtgc tccgcccgcc gtcccgacca
     8221 gcggagccgt ccctcgaggt ccggtccggc gccgagcgcg gtgatctgct cctcggtgcg
     8281 tcccgacgag aagaggatgc ggttcgcccc gcgagcggcc atgagcggcg cggcgagccc
     8341 gacgagcccg atgccgtgct gaacgtcgag ccaccagttc gtgatgccgg ggaaggcgcc
     8401 gttcagccgc gagacgtcga ggaaggagcg gaagttgctc cggatgaacc cgggccggtg
     8461 accgtgacga cgggcgtacg cctcggtgta tccccgcgcg gacccccagc cctcttggtt
     8521 gtcgagcgcg atctcgttgc cccagatcgt gatcagcagc tgcggctcgt ccaggaggag
     8581 cgacgtcgcc gtggagtcga cgccgccgct gaagagcacg gcggtcccgg cggctccggc
     8641 gacggggggc gcgtcgggcc cccgacggac cgttgccgcg tggagctgcc cgtcccagcg
     8701 cacgcgcggg tacagccgcc gataggtctc gcggatcggg ccgaggaccg aggcgagctc
     8761 ctcgtccatc tcctcgacgc gggcctccca gccggcggcc cacaggatcg gcgcgacgtt
     8821 cagcacgaac ggcaggagca gggtcgacga ggcgagcgtg cggaggtcga tgtcgcgctc
     8881 gtactcgacg acgaaatcct cacgcaggaa gcgttcggtg aacgcgcggt cggcgcggac
     8941 cctgatcgtc gaaccgcgga cctcgaggtc ctcgatcaac gacgggatcc ggctccccgg
     9001 aacctgggct gcggagctcg agcgggatct cacggtctcc ttccccgcgc cgcggcggga
     9061 ggggtcggtg ggccggcgcc atgatacccg cggccgaggc cggacccggt cggtagactg
     9121 cgcgcccgtg agccgagagc aggggcccga gacgcccacc gtcgaggaac ccgaccgggc
     9181 cgagggaggc gagccgggcg gcggagggcg gctcacccgc gcgctcctgg ccttcgggaa
     9241 ccggctgatc gcctgggttc gccgccggca cgcgaaccga tccccgagga cggagcggat
     9301 cctgctcgtc ctggcggtcc tgctgttcat cgtcgtgacc tggctcggct tccgcacgat
     9361 gcccgacgtg gggcggccgg tccggtgggg cgtcgtcgcc ctcgccgcgg ccgtcggcgt
     9421 tcccctctcc acgctcctga acgccttcga ataccgactg tccggcaaga tcctcggcca
     9481 ccggatcgcc ctcctcgagg cgatccgcgt gagcgtcctc gcctcggcgg cgaaccggct
     9541 gccgatcccc ggtgcggcgc tcgttcgcat cggggccctg cgccgcctgg ggtcgggctt
     9601 cgggaaggcg acgaccgcga ccgcggctgt cggcatcgtc tggaccggca cctcgttcgc
     9661 gacggccggg gtcctccagt gcgtcgcggc ccggtggatc ctcgggctca cgttcctcgg
     9721 cgggggcctc gccgggatcg gtctcggcta cgcgctgatg gtgtggcgcg cacgacccgc
     9781 gcgggcggtg cgcctgaccg ccgagcttct cctgatcgag ctggccgcgg tcgcgctccc
     9841 gaccgtgaag atgctcgtcg tgctccaggc gatcggcttc gacgtgacgg tcgcgcagtc
     9901 ggcggcgctc gccgtctcga tcgtcgtggc gcacgccacc gggttcttcc cgggtggtct
     9961 ggggctccgc gaggtgatcg ccggggcgat gagcccgctc gtcgggctgc cggtctccgt
    10021 gggcctcctg gggacggcag tcacgcgcgc cttcagcctc gtgatgtcgc tcctgatcgc
    10081 cgcgatcgtg ctgatcccgt acctgcgcgg caagaaagct tcagcggccg ccgaggccgt
    10141 gggcccaccg gttccccacg acgactgagg ctcagacgcg agccgtgcgg cgatcccgca
    10201 gccgcgcctg cagccgccgg agaccgcgct cgatcgcggg gaagcgtgcc acgactcggg
    10261 agatcctcgt tcgcatcgcc ccgatgattc tccaccgccg cgcgtaacgg ggaaaatcgc
    10321 gggagacgag ccagcggagg aaggggcgca cgtccgtgcc gtctccgagt ttccggggca
    10381 tgccctcctg cagcacgcgc cgcgcgtgcc gctgcatatc cgtccagtgg aagacgcgat
    10441 tcgcctgcgc caggatcagc gtgaagcgtc gcatctgccc gtcgatgacg cgggacagct
    10501 tcgccgggtc caccgggatc ccgtagcccc gggggtcctc cccctcgacg agcagcgccg
    10561 tcgacgtgcg aaggcacttc tcgcagcgac cgcagttctc tcctgaaccg tacttgtagg
    10621 agtagcaggg acgcagcatc gggcggactc cacccgagcg aacgtgcgcg acgatctccc
    10681 ggaccttatc gtgccgcgtc agctcgaacc cctcgtgcga cacctcggcg ccgccccaac
    10741 ggagctgtcc gtcggtgtca gggtgggacc cgtagggctc gtcggaagtg tgggtgtgcg
    10801 aggccgagac aaccagccgc cgcgacccct tcgccgccat cgcgggcgcc gcgagccccg
    10861 tgtagccgag gccgaaagcg accctggtcc accagtgcgg gatccccggc gacagcgcca
    10921 tgatcctcgc gggctcgagg aacgagcgga cgttgctccg gacgaacgcg gcctcgttcc
    10981 cggtgcgctc ggcgtgcgcg aggcacatgc ggcgcacctg cgcccacccg gcggtgttgt
    11041 cgagggcgat ccccgtcccc cagacggaga ccagcagctg ccgtcggtcg gcgttgcgca
    11101 ccgacgagtg ggtcgagtcg atcccggcgg tgaataggat cgtcacggga tcgtgtggag
    11161 ccccctcgag gtcgtggccg accagccgct ccgcgcggat ctcaccgctc cacgacaggt
    11221 gcgggtacag ccgctggaac gcggcgcgca cgcgacccag cgaggacgcc agctcctcgt
    11281 cgagctcggg gacggtgaag cgccggccgc tcagccacac gaccggggcg acgttcagca
    11341 ggaagggcag ctccagcacc caccgcggga cctgctcgag cgagatgccc tcgtactccg
    11401 cc
//
LOCUS       DATLGG010000053         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_65260, whole genome shotgun sequence.
ACCESSION   DATLGG010000053 DATLGG010000000
VERSION     DATLGG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65260"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VM638_01380"
     CDS             <1..431
                     /locus_tag="VM638_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11114.1"
                     /translation="SWAVATSWSDRGAPTEARARTGAALTNAQGAATASGAQPGEGTL
                     RDMGGADRYESDSDTTALSQPFTERTKGAIFSSAQGSVAGNSAALLHDRDAGRPDTFV
                     LTPTDAACLGTRGEGIWRDCGAGAGIGIVDDDARRLRTLG"
     gene            complement(498..1880)
                     /locus_tag="VM638_01385"
     CDS             complement(498..1880)
                     /locus_tag="VM638_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11115.1"
                     /translation="MLRMLLLIVLVLPLAPPAAAVPEPIQIPPARVGDRWSVDIANAD
                     LRYDLEVTPLGDAIDRWGRRRQAYPVRVEGFSPLSFIWMRVTRHHLHLDAASGVQIAD
                     VAEGVFDAGPSGTSTPGAYSTFWPNDTEAWPGWTLAGGLLAGRQLVPGSTIEVPLAVT
                     RTRGNERITLETEEIPPSRGARCARATGTAVYPQYFAYRDGITSDRGELRVSLEIVHC
                     DDSPFAVRVRNTYSGAFDHSVEASRIALDTGDGEAVPVGALDEPETHRLAARRPFDGS
                     MEDGGPLEIFGIEAAQDAAMLAPGLAAWRVAHPAAYLVEAEYIPARLDEPGATAGVGA
                     DRWRLTYAAPAGAQHQVSVEQRAGAAVPIDLPQATTYPVPVPPLATWPQEVVTPAGAI
                     ASWRQLAGAGARLNGLKWQPLLDHRWHVLSELPFDCPEDPCSTALSGLDLMFDVVTGA
                     TQEGLHPLGA"
     gene            1997..2917
                     /locus_tag="VM638_01390"
     CDS             1997..2917
                     /locus_tag="VM638_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11116.1"
                     /translation="MKLAAVHRRSGTVLGILVLLVLTLLPAPAGATTVSIVNLTRSGS
                     TVSVAGLASFSDEPFRLLGSDASGDSLPPGGELVGRDLVGFSMSTGVGDQLTLRWHLN
                     PLMATDRGQDVSYASAFCVGSGGGNCWVITFIDPAGTPVTNSFVWSWRCASESCAIGA
                     GDHIHSWETDRYDPQTKTALATLPFDALGITSGAQLRAVKPQHFGGEVYVTTPWWFLA
                     TAQTYPTWDGSSMAWTHRIATKDVSVAIGEPGQAPEQVAYPAPVRADEFNRYTVDLDV
                     SDRAPGDYAIYVRACFGANNCGYATRAITL"
BASE COUNT          460 a         1108 c         1168 g          464 t
ORIGIN      
        1 acagctgggc cgtggcgacc tcctggtcgg accgcggcgc tccgaccgag gcgagggcac
       61 gaacgggcgc ggcgctcacg aacgcgcagg gcgccgccac cgcctccggg gcccagccgg
      121 gtgagggcac cctgcgtgac atgggcggcg ccgaccggta cgagagcgac agcgacacga
      181 cggcgctgtc gcagcccttc accgagcgga cgaagggagc catcttctct tcggcccagg
      241 ggtcggtcgc gggcaacagc gccgcgctgc tccacgatcg cgacgctggc cgaccggaca
      301 cgttcgtcct cacgcccacc gacgccgctt gcctcggtac ccgcggcgag gggatctggc
      361 gcgactgcgg cgccggagcc ggcatcggga tcgtcgacga cgacgcccgg cggctccgca
      421 ccctcgggta gcgggcagct cctgcgagcc acccgggtca ggaggccgct cgccggcggg
      481 gcccaggcct cgacctttca cgcgcccagc gggtggagtc cttcctgcgt cgctcccgtg
      541 acgacgtcga acatcaggtc caagccggag agcgccgtgg agcacgggtc ttccggacag
      601 tcgaacggca gctcactcag gacgtgccaa cggtggtcga gcaggggctg ccacttcagc
      661 ccgttcaggc gtgcacctgc gccggcgagc tgtctccacg aagcgatcgc gcccgccggg
      721 gtcacgacct cctggggcca tgtcgcgagc ggcgggacgg gtaccgggta ggtcgtcgcc
      781 tgcggcaggt cgatcgggac ggccgcgcct gcccgctgct cgaccgacac ctgatgctgc
      841 gcccccgctg gtgccgcgta cgtcagccgc cagcggtcgg ctccgacacc ggcggtcgcc
      901 cccggttcgt cgagccgagc cgggatgtac tccgcttcca cgaggtaggc ggcaggatgt
      961 gcgacccgcc acgccgccag cccgggggcg agcatggccg cgtcctgcgc cgcctcgatg
     1021 ccgaagatct ccagcgggcc gccgtcttcc atcgacccgt cgaagggtcg ccgagcggcg
     1081 aggcggtggg tctcgggctc atccagggcg ccgaccggca cggcctcgcc gtccccggtg
     1141 tcgagcgcga tacgcgacgc ctcgaccgaa tggtcgaagg cgccgctgta ggtgttccgg
     1201 acgcggaccg cgaagggcga atcatcgcaa tggacgatct cgaggctcac gcgcagctcg
     1261 ccccggtccg aggtgatccc gtcccggtac gcgaagtact gcgggtagac ggccgtccca
     1321 gtcgctcgcg cgcagcgagc accgcgtgac gggggaatct cttcggtctc cagcgtgatc
     1381 cgttcgttgc cgcgggtgcg cgtgacggca agcggaacct cgatcgtgct gcccgggacg
     1441 agctggcgtc cggcgagcag gccaccggcg agcgtccagc cgggccacgc ctcggtgtcg
     1501 ttcggccaga aggtcgaata cgcgccgggc gtcgaggtgc cggagggccc cgcgtcgaag
     1561 acgccctcgg cgacatcggc gatctgcacc ccggaggcgg catcgaggtg aaggtgatgc
     1621 cgggtgaccc gcatccagat gaagctcagc gggctgaacc cctccaccct gacggggtag
     1681 gcctgccgcc ggcgtcccca gcggtcgatc gcatccccga gcggagtcac ctccaggtcg
     1741 tagcgaaggt ccgcgttcgc gatgtcgacg ctccaccgat cgccgacgcg cgccggcggg
     1801 atctggatcg gctcgggaac ggccgcggcg ggaggggcga gcggcagcac caggacgatg
     1861 aggaggagca tgcgcagcat ggtgcttgct tcgcctcgcc ccgagcggag tccttcgtgc
     1921 aactgcacaa accgggcagg tatcgagggc ctcgccgtcg aatcctctgc cgcggatcta
     1981 gccgaagggg gtcacgatga agctcgccgc cgtccatcgc cggtccggaa ccgttctcgg
     2041 gatcctggtg ctgctggtgc tgacgctgct gccggcgccg gccggagcga cgaccgtctc
     2101 gatcgtcaac ctgacgcggt caggttcgac cgtctctgtc gccgggctgg cctcgttcag
     2161 cgatgagccg ttccgcttgc tcggatcgga tgccagcggt gattccctcc caccgggcgg
     2221 cgagctcgtg ggtcgggacc tcgtggggtt ctcgatgtcg accggcgtgg gagaccagct
     2281 gacgctccgg tggcacctga acccgctgat ggcgacggac cgtggccagg acgtgtccta
     2341 cgcgagcgcg ttctgcgtcg ggtcgggcgg tggcaactgc tgggtcatca ccttcatcga
     2401 tcccgccggg acgccggtga cgaactcgtt cgtgtggagc tggcgatgcg cgagcgagtc
     2461 ctgcgcgatc ggcgccgggg accacatcca ctcgtgggag accgaccggt acgacccgca
     2521 gacgaagacg gctctcgcaa ccctgccgtt cgatgcgctg ggcatcacct ccggcgcgca
     2581 gctccgtgcc gtgaagccgc agcacttcgg cggcgaggtg tacgtgacga ccccgtggtg
     2641 gttcctggcg acggcgcaga cctatcccac gtgggacgga agctcgatgg catggacgca
     2701 ccggatcgcg acgaaggacg tctcggtcgc gatcggagag ccgggtcagg cgccggaaca
     2761 ggtcgcctat ccggcgccgg tgcgggcaga tgagttcaac cggtacacgg tcgacctcga
     2821 cgtgtcggat cgagcgcccg gtgactacgc gatctacgtc cgagcctgct tcggcgcgaa
     2881 caactgcggg tacgccaccc gcgcgatcac gctgtagcgg ggggtcgcgg agcggcctac
     2941 gccgtacgcg gaagggccta ccggccgtcg tcgcggtggg gtcgagtctc gtcggccggt
     3001 cggtcacctg cccgatgcgc ggggggcgcg atcgctggca gagtggtgcc cccagatcga
     3061 agggaggggt catgggatcc gcagaggcct tcgagctttc gaccgatgct gccgaatcgt
     3121 acgaggcgag gttcgtcccg acgctcttcg ccggttgggc cgcgcgggtc gtcgacgccg
     3181 ccgacctgtc tccgggggat
//
LOCUS       DATLGG010000054         4631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_83308, whole genome shotgun sequence.
ACCESSION   DATLGG010000054 DATLGG010000000
VERSION     DATLGG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83308"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VM638_01395"
     CDS             complement(<1..1274)
                     /locus_tag="VM638_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11117.1"
                     /translation="MEQGTEGRPPGRPSTLGGAAAPTEEPAGIPRLAEGVELLGRYEG
                     SGFKEPPYIAKRGDGQMVQLPPLLYAVAEEIDGGSDVAAIAARVSARIRREIEPQDLA
                     FLIEEKLAPLGVVAARSGEAEPVAAAADPMLALKLRTKVVPKRAVRAIATIFQPLFFP
                     LVIVAVLAGLVVLDWWLFFDHGVAQSLRAAIYQPLLLLLVLGMVVISAAFHESGHAAG
                     AKYGGADPGEMGVGIYIVWPAFYTNVTDTYRLGKGGRLRTDLGGVYFNAIFILLTSGV
                     YFLTRFEPLLLVIVVQHLEIVRQLLPVVRLDGYYILSDLTGVPDMFSRIKPVLVSLLP
                     WRETDPRVAELKVWPRVVVTFWVTLVVPLLAFQLALIVVHAPRIVATALDSLARLGGT
                     VSSAFGDGRPGAAVVGILQMLLLVLPLAGVAYT"
     gene            complement(1284..1490)
                     /locus_tag="VM638_01400"
     CDS             complement(1284..1490)
                     /locus_tag="VM638_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11118.1"
                     /translation="MTEEYRKLTREDLEQLAGEELPERAAMSLINANVAAPVNIALAA
                     NVLSDNSIAYANAEQTADIDQSAG"
     gene            complement(1500..1706)
                     /locus_tag="VM638_01405"
     CDS             complement(1500..1706)
                     /locus_tag="VM638_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11119.1"
                     /translation="MTEEYRKLTREELEQLAGEELPERAAMSLINANVAAPVNAAIAL
                     NVLSDNSVAYANAEQDVDITQSAG"
     gene            complement(1715..1933)
                     /locus_tag="VM638_01410"
     CDS             complement(1715..1933)
                     /locus_tag="VM638_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11120.1"
                     /translation="MSGLGEEQYRKLGREELEQLAGEALPERAAMSLVNANIAIPVNA
                     ALAANVLSDSAIAYADASQTTPIDQDAG"
     gene            complement(1930..2121)
                     /locus_tag="VM638_01415"
     CDS             complement(1930..2121)
                     /locus_tag="VM638_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11121.1"
                     /translation="MRFRRIIRKQVRRSVDGGNVAADVNAVIAGNVGEGRAHTRVSSK
                     QRIVQRTENREDDDPNARR"
     gene            complement(2168..2572)
                     /locus_tag="VM638_01420"
     CDS             complement(2168..2572)
                     /locus_tag="VM638_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HVM11122.1"
                     /translation="MADAHLARELGVTRGRFRTGEIRRRRFERLVADAIEGLPSWVHE
                     RLENVDVVVEDWPPEDRPDLLGLYEGIPLTERGSTYAGVLPDRITLYRRTIEAEARTD
                     EELKRAVRQTVAHEIAHFFGITDERLREIDRY"
     gene            complement(2536..4011)
                     /locus_tag="VM638_01425"
     CDS             complement(2536..4011)
                     /locus_tag="VM638_01425"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HVM11123.1"
                     /translation="MSDASRRAVRATLVVVAITALAGYARFHGLGLPDEKVFDEVYYA
                     SDGCWYAGLDPEACGLEDDREQSWVHPPLGKIMIGWGIDAFGNDPFGWRFSAAVAGTL
                     AVALGGILAFLLTSSPAWAGIGALLVGTEHLLFVQSRIAMLDVFLALLVLLGLVLLVW
                     DRRRNLLDAAPEPTTPPPPGDATEDAWLDQAPEDQGWLDEPVDEAPAAPGGLRPLRFL
                     AGAAFGAAVAVKWSGVLALVAGVLLATAWERSDRRARGVRRPLRRALAEGWPSLLLGL
                     VVVPLLVYSLAWSSWFLREPERSVGSWIGHHQAMADYHFGLSTEDEDGEPIHPYMSRA
                     PSWLLLLRPVAYFFESGSWGQPSATAREILGIGSPLLFWGGLVVTPYLLLAWFRRRDW
                     RYGAVLVPLLLQYLPWLAVSRPLFLFYMTPVTPLLALGMLLAVRDLGHVRIRGRRIMA
                     AAPAVLVAVAVALFAFFWPVLTARTLSADAWQTRIWLGSWV"
     gene            4037..>4631
                     /gene="rsmI"
                     /locus_tag="VM638_01430"
     CDS             4037..>4631
                     /gene="rsmI"
                     /locus_tag="VM638_01430"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HVM11124.1"
                     /translation="MPRPGTLWLVGTPIGNLKDITDRAREVLAAADVIACEDTRRVRT
                     LLTHLGIRPTRLLSFYEGNERKRVSEVLGHLRDGWDVALVSDAGMPALSDPGYRLVAA
                     CVAEGFEVDAAPGPTAVVTALVLSGLPTDRFAFEGFLPRKPGPRRERLASLAAEERTL
                     VFYESPRRIHELLTDAAAALGDRRAALAREMTKMHQEV"
BASE COUNT          710 a         1641 c         1640 g          640 t
ORIGIN      
        1 gtgtaggcga cgccggccag cggaaggacc aggagcaaca tctgcaggat gccgacgacc
       61 gcggcgccgg ggcgtccgtc gccgaacgcc gaggagaccg tgccgccgag tcgcgcgagc
      121 gaatcgagag ccgtcgcgac gatccgcggc gcgtgcacga cgatcagggc gagctggaag
      181 gcgagcagcg ggacgacgag cgtcacccag aacgtcacga cgacgcgcgg ccataccttc
      241 agctcggcga cgcggggatc ggtctcgcgc cacggcagga ggctgacgag cacgggcttg
      301 atccgcgaga acatgtccgg cacgcccgtg aggtcgctca ggatgtagta gccgtcgagc
      361 cgaacgaccg gcaggagctg gcggacgatc tcgaggtgct ggaccacgat cacgagcagc
      421 agcggctcga accgcgtgag gaagtacacg cccgacgtca gcaggatgaa gatcgcgttg
      481 aagtacaccc caccgaggtc ggtccgcagg cgaccgccct tgccgaggcg gtacgtgtcg
      541 gtgacgttcg tgtagaaggc cggccagacg atgtagatgc cgacccccat ctcaccggga
      601 tcggcgccgc cgtacttcgc cccggcggca tgcccgctct cgtggaaggc cgcggagatg
      661 accaccatcc cgaggacgag cagcaggagc aggggctggt agatcgccgc tcggaggctc
      721 tgcgcgacgc cgtggtcgaa gaagagccac cagtcgagga cgacgagtcc cgcgagcacc
      781 gcgacgatca cgagcgggaa gaagagcggc tggaagatcg tcgcgatggc cctcacggcg
      841 cgcttcggga cgaccttcgt gcggagcttg agcgcgagca tcggatcggc cgcggccgcc
      901 acgggctccg cctcgccgga tcgggcggcg acgaccccga gcggagcgag cttctcctcg
      961 atcaggaacg cgaggtcctg cggttcgatc tcgcgtcgga tcctcgccga gacccgcgcg
     1021 gcgatcgcgg cgacgtcact gccgccgtcg atctcctccg cgaccgcgta caggagggga
     1081 gggagctgga ccatctggcc gtccccgcgc ttcgcgatgt acggcggctc cttgaagccc
     1141 gacccctcgt agcggccgag cagctcgacg ccctcggcga gccgcgggat gccggccggc
     1201 tcctcggtgg gcgcggccgc tccccccagc gtcgaggggc ggcccggagg ccgcccctcg
     1261 gttccctgct ccatcgcgac gggttagccc gcgctctggt cgatgtctgc cgtctgctcg
     1321 gcgttcgcgt acgcgatcga gttgtccgag aggacgttgg cagcgagcgc gatgttcacc
     1381 ggcgctgcga cgttcgcgtt gatcagcgac atcgccgctc gctccggcag ctcctcgccc
     1441 gcgagctgct cgaggtcctc ccgcgtcagc ttcctgtact cctcggtcat ccgatcctcc
     1501 tatccggcgc tctgggtgat gtcgacgtcc tgctcggcgt tcgcgtacgc caccgagttg
     1561 tccgacagga cgttcagggc gatcgcggcg ttcaccgggg ccgcgacgtt cgcgttgatc
     1621 agggacatcg ccgcccgctc gggcagctcc tcgcccgcga gctgctccag ctcttcgcgc
     1681 gtcagcttcc tgtactcctc ggtcatcgct cctcctatcc ggcgtcctgg tcgatcggcg
     1741 tggtctgcga tgcgtccgcg taggcgatcg cgctgtcgga cagcacgttc gctgcgagcg
     1801 ccgcgttcac ggggatcgcg atgttcgcgt tcacgagcga catcgcggcg cgctccggca
     1861 gtgcctctcc ggcgagctgc tcgagctcct cgcggccgag cttgcggtac tgctcctcac
     1921 ccaacccact cacctcctcg cgttcgggtc gtcgtcctcg cggttctcgg tccgctggac
     1981 gatccgctgc ttgctggaga cgcgcgtgtg cgcccgaccc tccccgacgt tccccgcgat
     2041 caccgcgttg acgtcggcgg cgacgttccc cccgtcgacc gaccggcgga cctgcttgcg
     2101 gatgatccgg cggaacctca cgaaccgcgc tattccccgg cggcgcgtgc agcaaacgga
     2161 ccggacgcta gtagcggtcg atctcgcgga gccgctcgtc ggtgatgccg aagaagtgtg
     2221 cgatctcgtg cgccacggtc tgtcgcacgg cgcgcttgag ctcctcgtcg gtccgtgcct
     2281 cggcctcgat cgttctgcgg tagagggtga tgcggtccgg aaggacgccg gcgtaggtgc
     2341 tccctcgctc ggtgaggggg atcccctcgt agagtccgag caggtcggga cggtcttccg
     2401 gcggccagtc ctcgacgacg acgtcgacgt tctcgaggcg ctcgtgcacc caggagggca
     2461 ggccttcgat cgcgtcggcg acgagccgct cgaaccgccg cctccggatc tcgcccgtgc
     2521 ggaaccggcc gcgcgtcaca cccagctccc gagccagatg cgcgtctgcc acgcgtccgc
     2581 actcaacgtc cgtgcggtca gcaccggcca gaagaaggcg aacaacgcga cggcgaccgc
     2641 gacgagcacg gccggtgccg cggccatgat ccgccgcccc cggatgcgga cgtgccccag
     2701 atcccggacg gccaggagca tcccgagcgc gagcagcggc gtgacgggcg tcatgtagaa
     2761 gaggaagagc ggccgcgaca cggcgagcca tgggaggtac tgcagcagga gcgggacgag
     2821 gaccgcgccg tagcgccagt cgcgccggcg gaaccaggcg agcagcaggt agggggtcac
     2881 gaccagccct ccccagaaca gcagcgggct cccgatcccg aggatctcgc gcgcggtggc
     2941 cgatggctgc ccccagctcc ccgattcgaa gaagtaggcg acgggacgca ggagcagcag
     3001 ccacgacggc gctcgcgaca tgtacggatg gatcggctcc ccgtcctcgt cctcggtcga
     3061 gaggccgaag tggtagtccg ccatcgcctg gtggtggccg atccaggatc cgaccgagcg
     3121 ctccggctcg cggaggaacc aggacgacca agcgagggag tagacgagga ggggcacgac
     3181 gacgagcccc aggaggagcg acggccatcc ctcggcgagg gcgcgccgga gcggccgccg
     3241 cacgccgcgc gcccggcggt cactgcgctc ccatgccgtg gcgagcagga cccccgccac
     3301 gagggccagg acgccggacc acttcaccgc gaccgccgcc ccgaacgccg ccccggcgag
     3361 gaaccgcagg ggacgaaggc cgcccggcgc ggctggagct tcgtccacgg gttcgtcgag
     3421 ccatccctgg tcctcgggcg cctggtcgag ccacgcatcc tccgtcgcgt cgccgggcgg
     3481 cggcggggtc gtcggctcgg gcgcagcgtc gagcaggttc ctgcggcggt cccagaccag
     3541 gaggacgaga ccgagcagca cgagcagggc caggaagaca tcgagcatcg cgatcctcga
     3601 ttgcacgaac agcaggtgct cggttccgac gagcagcgca ccgatgccgg cccaggccgg
     3661 gctcgacgtc agcaggaagg cgaggatccc accgagcgcg acggccaggg ttcccgcgac
     3721 cgccgcggag aagcgccacc cgaacgggtc gttgccgaag gcgtcgatac cccacccgat
     3781 catgatcttg ccgagcggcg ggtggaccca cgactgctcg cggtcgtcct cgaggccgca
     3841 tgcctccggg tcgagccccg cgtaccagca gccgtcggag gcgtagtaga cctcgtcgaa
     3901 gaccttctcg tccggcaggc cgaggccgtg gaaccgcgcg tagcccgcga gcgcggtgat
     3961 cgcgaccacg accagggtgg cgcggacggc gcgccgggag gcatcgctca tggggcacca
     4021 ggatagggtg ggcacgatgc cccggcccgg aacgctgtgg ctggtcggaa ccccgatcgg
     4081 caacctcaag gacatcaccg atcgcgcgag ggaggtgctc gcggcggccg acgtcatcgc
     4141 ctgtgaggac acgcgccggg tgcggacgct gctgacccac ctcggcatcc gaccgacccg
     4201 gctgctctcc ttctacgagg ggaacgagcg gaagcgggtc tcggaggtgc tcggccacct
     4261 gcgggatgga tgggacgtcg ccctcgtgag cgacgccggg atgccggccc tctccgaccc
     4321 cggctaccgt ctcgtcgcgg cctgcgtcgc cgaggggttc gaggtggatg ccgctcccgg
     4381 cccgaccgcc gtcgtcacgg cattggtcct ctcgggtctg ccgaccgatc gcttcgcctt
     4441 cgagggtttc cttccgcgga agcccggtcc gaggcgcgag cgcctcgcgt ccctcgcggc
     4501 cgaggagcgc acgctcgtct tctacgaatc tccgaggcgg atccacgagc tcctgacgga
     4561 tgccgccgcg gcgctcggtg accggcgcgc cgcgctcgcg cgggagatga cgaagatgca
     4621 ccaggaggtc c
//
LOCUS       DATLGG010000055         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_98095, whole genome shotgun sequence.
ACCESSION   DATLGG010000055 DATLGG010000000
VERSION     DATLGG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98095"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VM638_01435"
     CDS             complement(<1..287)
                     /locus_tag="VM638_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11125.1"
                     /translation="MHRRHVAVSSLALAWALLVATPATALSPVPPLDSNAPQPSEAST
                     VEETPQEAEVTPGRGVPVLQAGDRGRKVKVLQVRLQRVGVRDMPVSGLYGE"
     gene            complement(411..884)
                     /locus_tag="VM638_01440"
     CDS             complement(411..884)
                     /locus_tag="VM638_01440"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVM11126.1"
                     /translation="MASNTLRATVAGAMCTTLLAATMVAAPAAEARPKMPRACYSGKI
                     VCVDKSKRMIRLLVNGKTIVRMDARFGSSRTPTRNGMFRVYWKNANHVSSLFGSAMPY
                     AMFFSGGQAIHYSSDFARRGYSGASHGCVNTRDYGRMRTLFNNTPTGTRVYIYYW"
     gene            complement(1075..1545)
                     /locus_tag="VM638_01445"
     CDS             complement(1075..1545)
                     /locus_tag="VM638_01445"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVM11127.1"
                     /translation="MASNRKRAAGVGFAASALIGASFIALPSEASARPAACNTGRVIC
                     VNKSTRQLALVVNGRTVIAMSARFGSVSRGFPTREGMFRVYWKNANHVSSLYGSAMPF
                     SLFFSGGQAIHYSSDFARNGYAGASHGCVNTRDYSATRALFNATPVGTKVYIHW"
     gene            1778..>2617
                     /locus_tag="VM638_01450"
     CDS             1778..>2617
                     /locus_tag="VM638_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM11128.1"
                     /translation="MPRRVATLSVHTSPLDQPGTGDAGGMNVYVVEVAKRLAQAGVEV
                     EIFTRTTSSDLPPVVELAPGVTVRHVTAGPFEGLAKEDLPAQLCAFTTGVLRAEATRD
                     PGYYDLVHSHYWLSGQVGWLAAERWGLPLIHSMHTMAKVKNATLASGDVPEPVSRVIG
                     ETQVVEAADRLVANTAEEADQLVELYGAERARVDVVSPGVDLEVFVPGRRSAARVELG
                     IADTDDVILFVGRIQPLKAPDVLLGAVAKLVGNDLARRHRTIVAVVGGPSGTGTAEPE
                     QLQK"
BASE COUNT          409 a          875 c          903 g          430 t
ORIGIN      
        1 tcctcgccgt acagaccgga gacgggcatg tcccgcacgc cgacccgctg cagccggacc
       61 tgcaacacct tgaccttgcg gccgcggtca ccggcctgaa gcaccggcac cccgcggccg
      121 ggcgtgacct cggcctcctg cggagtctcc tcgaccgtgc tcgcctccga cggctggggg
      181 gcgttcgaat cgagcggggg gacgggtgag agcgccgtcg ccggggtggc gaccagcaga
      241 gcccaggcca gagcgaggga actgaccgcg acgtgccgtc ggtgcatgca aggtcccggg
      301 gttgctgggt acatgacatc ggccgcccgc agacacggac ggccgaggtc gattctacga
      361 gtggttcgac agttcccgcc ctgggaatgc cctgggaccc acctcgcggc tcaccagtag
      421 tagatgtaga cccgggtgcc ggtcggggtg ttgttgaaca gcgtgcgcat ccggccgtag
      481 tcgcgggtgt tcacgcagcc gtgcgaagca cccgagtagc cgcggcgggc gaagtcggag
      541 gagtagtgga tcgcctgacc gccgctgaag aacatggcgt acggcatcgc actgccgaac
      601 agggaggaca cgtggttcgc gttcttccag tacacgcgaa acatgccgtt gcgggtcggc
      661 gtacggctgg agccgaagcg ggcgtccatg cgcacgatcg tcttgccgtt gacgagcagg
      721 cggatcatcc gcttcgactt gtcgacgcag acgatctttc cgctgtagca cgcccgcggc
      781 atcttcggcc gggcctccgc cgccggggcg gcgaccatcg tggcggcgag cagagtggtg
      841 cacattgcgc ccgcgacggt cgcgcggagt gtgttcgacg ccatggtgga actcctcggg
      901 tacaggatcg tgaacccgag tgttcttcat cgacccggac gctccggctc ggattgccaa
      961 atgcccgatt cgcctttaac gatcactgcg gccatttccg cttcggctgg cgcgtcgctg
     1021 acctgatgat cgggtcaaga tcacctggcc gtcctcgccg cctcagcggc tgcctcacca
     1081 gtggatgtag accttggtcc ccaccggcgt cgcgttgaac agcgcccgcg tcgcggagta
     1141 gtcgcgggtg ttgacgcagc cgtgcgaggc gccggcgtac ccgttacggg cgaagtccga
     1201 cgagtagtgg atggcctggc cgccactgaa gaacaacgag aacggcatcg cgctgccgta
     1261 aaggcttgag acgtgattcg cgttcttcca atagacgcgg aacatgccct cgcgggtggg
     1321 gaagccccgg ctgaccgagc cgaagcgggc cgacatcgcg atgaccgtgc ggccgttcac
     1381 gaccagggcg agttgccggg tggacttgtt cacgcagatc acccgccccg tgttgcaagc
     1441 cgccgggcgc gccgacgcct ccgacggcag cgcgatgaac gaggcaccga tcagagcgct
     1501 tgcggcgaat ccgacgccgg cggcgcgctt gcggttggag gccattaccg actccttgat
     1561 gatctaaaac cagagtgctg ttcgggtctg ctgccccgaa cgctataggc gtatgtgtgt
     1621 tttgcaccag atgtgtggtt tggtccacac gcggtacgac gctttgggac gctgtccggt
     1681 tgaccggaat gcgcgaggat ctgctggggt tgcccaaccg ggtcggaccg tgatcgcgga
     1741 ggtgtagtca gtgcgcgtgc tgccggttcc cggagtcatg ccgcgtcgcg tcgccaccct
     1801 ctcggtccac acctcgcccc tcgatcaacc gggcaccggc gacgccggcg ggatgaacgt
     1861 ctacgtcgtc gaggtggcca aacgactggc gcaggccggt gtcgaggtcg agatcttcac
     1921 gcgtacgacc tcctcggact taccgcccgt ggtcgaattg gcacccggcg tcaccgtgcg
     1981 gcacgtgacc gccggcccct tcgagggcct ggccaaggaa gatcttccgg cccagctctg
     2041 cgccttcacg accggagttc tgcgcgccga ggcaacgcgg gatccgggct actacgacct
     2101 cgtccactcg cactactggc tctccggcca ggtggggtgg ttggccgcgg agcgctgggg
     2161 gctgcccttg atccactcga tgcacacgat ggcgaaagtg aagaacgcca cactcgcctc
     2221 gggcgatgtt ccggagccgg tctcccgcgt catcggggag acccaggtcg tggaggcggc
     2281 cgatcggctg gtcgcgaaca ccgccgagga ggcggaccag ctcgtcgagc tctacggcgc
     2341 cgagcgcgcc cgggtcgacg tcgtctcccc gggcgtcgac ctggaggtgt tcgtgcccgg
     2401 ccgccgttcc gccgcccggg tcgagctggg catcgccgac accgacgacg tcatcctgtt
     2461 cgtcggccgg atccagccgc tcaaggcccc cgacgtcctg ctcggcgccg tcgcgaagct
     2521 cgtcgggaac gacctggccc ggcgccaccg cacgatcgtc gccgtcgtcg gcggcccgag
     2581 cggcaccggt acggccgaac ccgagcagct gcagaag
//
LOCUS       DATLGG010000056         6164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_113279, whole genome shotgun sequence.
ACCESSION   DATLGG010000056 DATLGG010000000
VERSION     DATLGG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113279"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VM638_01455"
     CDS             <1..764
                     /locus_tag="VM638_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVM11129.1"
                     /translation="YSAVDPFVEELVCRELGLQPAEIATQVVQRDRHAELLSAIAVTG
                     ATLETIAVEVRHLARTEVREVQEPFGKGQKGSSAMPHKRNPILCERITGIARLLRANA
                     HAGLENVALWHERDISHSSVERVILPDSTILLDYAMHLATKVVRGLEVYPKRMRENLD
                     ASGGLVYSQPVLLALIDRGLSRDDAYAIVQAAAAAAWDEGAGFRERLLADERVPLTEA
                     ELAPLFEPRLEHLDAVFARLEKLEIDTTSPEGGDG"
     gene            761..1657
                     /locus_tag="VM638_01460"
     CDS             761..1657
                     /locus_tag="VM638_01460"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVM11130.1"
                     /translation="MSVLAEMAAQGKVRDIYDVGDDRLFMVASDRISAFDVVLPNPIP
                     DKGRVLTGLSLFWFEKTKGIMGNHLISAEVDAFPDAFGDHATELAGRSMLVKKAKMLP
                     IECVARGYISGSGWKEYRRDGTVCGIRLPEGLQESDRLPEPIFTPATKAETGHDENIT
                     FEQAAEIVGEGLAQRCRELTLELYSFARDLAAERGIILADTKFEFGFADGELILCDEV
                     LTPDSSRFWPADRYEPGGAQPSFDKQYVRDWLDESGWDHEPPAPDLPGEVVDASRARY
                     VEAYERITGEAFDDYLRRMGAE"
     gene            1654..1911
                     /gene="purS"
                     /locus_tag="VM638_01465"
     CDS             1654..1911
                     /gene="purS"
                     /locus_tag="VM638_01465"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HVM11131.1"
                     /translation="MKRRFEVVVRLKEGLLDPQGKTVEDALPRMGWTNVSEVRVGKLV
                     EMTVDAEDEEAARSTAEEVASRLLANPVIEDVRLLSMEEAG"
     gene            1908..2615
                     /gene="purQ"
                     /locus_tag="VM638_01470"
     CDS             1908..2615
                     /gene="purQ"
                     /locus_tag="VM638_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVM11132.1"
                     /translation="MTKPRVAVVTFPGSNDDRDALRAVEQMGGEPVSVWHRDTDLRGA
                     DAVILPGGFAYGDYLRTGAIAAFAPVMEAVREHAEAGRPVLGVCNGFQILCEARLLPG
                     ALIRNKTLSFVHRRVHVRVETLRSAFTSSAEPGDVLEIPIKHGEGQYVADDATLDRLE
                     SEGQVVFRYCTPEGAVTEDANPNGAMRNIAGIRNEAGNVVGLMPHPEHAVDPDVGPVG
                     GQVLFESLLSSLVGAAR"
     gene            2612..4813
                     /gene="purL"
                     /locus_tag="VM638_01475"
     CDS             2612..4813
                     /gene="purL"
                     /locus_tag="VM638_01475"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HVM11133.1"
                     /translation="MTEPLHRQLALTDDEYERIVGTLGREPNRAELAMYAAMWSEHCS
                     YKSSKVHLKTLPTEGERVLVGPGEDAGVIDIGEGLACVFKMESHSHPSAIEPFQGAAT
                     GVGGIIRDVLSMGARPVALLDPLRFGPLDDARNRWLVGGVVSGIGQYGNSIGVPTVGG
                     EVKFASAHTQNPTINVMCVGLAPADRLVRAKAEGEGNLLVLVGAHTGRDGIGGVSVLA
                     SRTLDETSEEQRPSVQIGDPFYEKLLIEACLEMIEADVLVGLQDLGGAGLVCAVSESA
                     ARGGVGADLDLDAVPLRERAMEDFEILTSESQERMLTIVRPDDLDAVRAICEKWGLVS
                     AVVGRVAPGGALRAKRNGSVVAEVPARSLADEGPEYRRPMERPASIEALNADDPTVAP
                     PPGDLRDALLRVLASPNVASKRWVWEQYDSIVQGNTVAGPGGSAAILRIQDTMRAIAL
                     STDGNGRYAHLDPYLGGAHAVAEAARNVACAGAVPVAITNCLNFGNPERPEVIWAFAE
                     VVRGMGDACRALGTPVTGGNVSFYNETGDSAIWPTPVVGMLGLLDDYRFSLRPAFGPD
                     QVVYLLGETHAELGGSEYADVVLGMVSGLPPALDLDREAALHRVLAEGARTEVIAAAQ
                     DCSDGGLAVALLEAAINGDTGVAATLPGDLPPHVTMFSESSSRAVVAVAPEKAAELED
                     LADRHGLPWTRLGDTGGPRIVIGEVDLTVAEARQIYEGAIPALLQMHAQAG"
     gene            4885..>6164
                     /gene="purF"
                     /locus_tag="VM638_01480"
     CDS             4885..>6164
                     /gene="purF"
                     /locus_tag="VM638_01480"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HVM11134.1"
                     /translation="MGGESPKEECGLFGVWAPGEDVSRLIYFGLYALQHRGQESAGMA
                     ISDGSNMLVYRELGLVSQVFNERTLSTLQGDLGIGHTRYSTTGSTTWENAQPIFKTDG
                     GRALALAHNGNLVNTQDLADRVGFLGRSTSDTDLVATLLSREAEDGLEAAALKVLPTL
                     QGAYCFLMSDEKTVYAARDPLGIRPLVIGRLPNGFCFASETAALDIVGAAYVRDVEPG
                     ELVMVDERGLRSVRFAESPREALCLFEFVYLARADTTMMGTTVHRARREMGKALAAEA
                     PVEADLVIPIPDTASSAAQGYAEASGIPYGEGLMKNRYVHRTFIQPTPGLRQMGVRLK
                     LNPVPDVIRGRRLIVVDDSIVRGTTTRQIVQLLREAGATEVHMRITSPPVRWPCFYGI
                     DMSTRQELVASDLSVDEVREFIGADSLSYLTLPSL"
BASE COUNT          898 a         2167 c         2219 g          880 t
ORIGIN      
        1 cctactcggc ggtcgatccg ttcgtcgagg agctcgtctg ccgcgagctc gggctccagc
       61 cggccgagat cgcgacccag gtcgtgcagc gtgaccgcca cgcggagctg ctgtcggcga
      121 tcgcggtcac cggcgccacc ctcgagacga tcgcggtcga ggtccgccac ctcgcgcgga
      181 cggaggtccg ggaggtgcag gagccgttcg gcaaggggca gaaggggtcg tccgcgatgc
      241 cgcacaagcg caacccgatc ctgtgcgagc gcatcaccgg gatcgcgcgg ttgctgcgcg
      301 cgaacgctca cgcgggactc gagaacgtcg cgctgtggca cgagcgggac atctcccact
      361 cgtcggtgga gcgcgtgatc ctgccggact cgacgatcct gctcgactac gcgatgcacc
      421 tcgcgacgaa ggtcgtgcgc gggctcgagg tgtacccgaa gcggatgcgt gagaacctcg
      481 atgcgtcggg cgggctcgtc tactcccagc cggtcctgct cgccctcatc gaccgcggcc
      541 tgagccgcga cgacgcgtac gcgatcgtcc aggccgcggc cgcggccgcc tgggacgagg
      601 gagcgggctt ccgcgagcgg ctgctcgccg acgaacgggt cccgctcacc gaggcggagc
      661 tcgccccgct gttcgaaccg cggctcgagc acctcgacgc ggtgttcgcg cggctggaga
      721 agctggagat cgacacgact tccccggaag gaggggacgg atgagcgtgc tggcggagat
      781 ggcggcgcag ggcaaggtcc gcgacatcta cgacgtgggg gacgaccggc tcttcatggt
      841 ggcgagcgac cggatctcgg cgttcgacgt cgtgctgccg aacccgatcc cggacaaggg
      901 ccgcgtcctc acggggctgt cgctgttctg gttcgagaag acgaagggga tcatgggcaa
      961 ccacctgatc agcgcggagg tggatgcctt ccccgacgcc ttcggcgatc acgcgaccga
     1021 gctcgcgggt cgctcgatgc tcgtgaagaa agcgaagatg ctcccgatcg agtgcgtcgc
     1081 ccgcggctac atctcgggct cgggctggaa ggagtaccga cgggacggga ccgtctgcgg
     1141 gatccggctc ccggaggggc tccaggagtc cgaccgtctg ccggagccga tcttcacgcc
     1201 ggcgacgaag gccgagacgg ggcacgacga gaacatcacg ttcgagcagg cggcggagat
     1261 cgtcggtgag gggttggccc agcgctgccg tgagctcacg ctcgagctct actccttcgc
     1321 gcgcgacctc gccgccgagc gcggcatcat cctcgcggac acgaagttcg agttcggttt
     1381 cgccgacggc gagctgatcc tgtgcgacga ggtgctgacg ccggactcct cgcggttctg
     1441 gcccgccgac cgctacgagc ccggcggcgc gcagccgtcg ttcgacaagc agtacgtccg
     1501 cgactggctc gacgagagcg gctgggacca cgagcctccg gctccggacc ttccgggcga
     1561 ggtcgtggac gcgagccgcg cccgctacgt cgaggcgtac gagcggatca cgggcgaggc
     1621 cttcgacgac tacctccgga ggatgggcgc cgagtgaagc gccgcttcga ggtcgtcgtc
     1681 cggctcaaag agggcctcct cgacccccag gggaagacgg tggaggacgc gctcccgcgc
     1741 atgggctgga cgaacgtttc ggaggtccgc gtcggcaagc tcgtggagat gacggtggat
     1801 gccgaggacg aggaggccgc gcgtagcacg gcggaggagg tggcgagccg cctgctggcg
     1861 aaccccgtga tcgaggacgt gcggctgctc tcgatggagg aggcggggtg acgaagccgc
     1921 gggtggcggt ggtcaccttc cccgggtcca acgacgaccg cgacgccctc cgagcggtgg
     1981 agcagatggg cggagagccc gtctcggtct ggcaccgcga caccgatctc cggggggccg
     2041 acgcggtgat cctcccgggc ggcttcgcct acggcgacta cctccgaacc ggggcgatcg
     2101 cggcgttcgc acccgtcatg gaggccgtgc gcgagcacgc cgaggcgggc cggcccgtcc
     2161 tcggggtctg caacgggttc cagatcctct gcgaggcgcg cctcctcccc ggagcgctga
     2221 tccgcaacaa gacgctctcg ttcgtccacc ggcgcgtgca cgtgcgcgtc gagaccctgc
     2281 gctcggcgtt cacgtcttcg gcggagcccg gagacgtgct ggagatcccg atcaagcacg
     2341 gcgaggggca gtacgtcgcc gacgacgcga cgctcgaccg cctcgagtcc gaggggcagg
     2401 tggtgttccg ctactgcacg cccgagggag cggtgaccga agacgcgaac cccaacggtg
     2461 cgatgcgcaa catcgccggg atccgcaacg aggccgggaa cgtcgtcggt ctgatgccgc
     2521 accccgagca cgcggtggac cccgacgtcg gccccgtggg ggggcaggtg ctgttcgagt
     2581 cgctcctctc ctcgctggtg ggcgccgcga ggtgaccgag ccgctccacc gtcagctcgc
     2641 gctgacggac gacgagtacg agcggatcgt cgggaccctc gggcgggagc cgaaccgcgc
     2701 cgagctcgcg atgtacgcgg cgatgtggtc cgagcactgc tcctacaagt cgagcaaggt
     2761 ccacctgaag acgctgccca ccgagggcga gcgcgtcctg gtcgggcccg gcgaggacgc
     2821 cggcgtgatc gacatcggcg aggggctcgc ctgcgtgttc aagatggagt cgcactccca
     2881 cccctcggcg atcgagccgt tccagggggc ggccaccggc gtcggcggga tcatccgcga
     2941 tgtgctgtcg atgggtgcgc gacccgtcgc cctcctcgac ccgcttcgtt tcgggccgct
     3001 cgacgacgcg cgcaaccgct ggctggtggg aggcgtggtc tccggcatcg gccagtacgg
     3061 caactcgatc ggcgtcccga cggtcggtgg agaggtgaag ttcgcgtcgg cgcacacgca
     3121 gaacccgacg atcaacgtga tgtgcgtcgg cctcgcgccg gccgaccgcc tcgtccgcgc
     3181 caaggccgag ggggagggga acctgctcgt cctcgtcggg gcgcatacgg gccgcgacgg
     3241 gatcggaggg gtgagcgtgc tcgcctcccg caccctcgac gagacctcgg aggagcagcg
     3301 tccgagcgtg cagatcggcg accccttcta cgagaagctc ctgatcgagg cctgcctcga
     3361 gatgatcgag gcggacgtgc tcgtgggcct gcaggacctc ggcggagcgg gcctcgtctg
     3421 cgccgtctcg gagtcggcgg cgcgtggcgg cgtcggcgcc gacctcgacc tcgacgcggt
     3481 tccgctgcga gagagggcga tggaggactt cgagatcctc acgtcggagt cgcaggagcg
     3541 catgctcacg atcgtgcgcc cggacgacct cgatgcggtg cgggcgatct gcgagaagtg
     3601 gggcctcgtc tccgccgtcg tcggccgggt cgcccccggt ggcgcgctcc gggcgaagcg
     3661 gaacggctcc gtggtcgccg aggtgccggc gcgctcgctc gccgacgagg gtcccgagta
     3721 ccggcgcccg atggagcggc ccgcctcgat cgaagccctc aacgccgacg accccaccgt
     3781 ggcgcctccc ccgggcgacc tgcgtgacgc cctgctgcgg gtgctcgcct ccccgaacgt
     3841 cgcctcgaag cgctgggtgt gggagcagta cgactcgatc gtccagggga acacggtcgc
     3901 cggcccgggt gggtcggccg cgatcctgag gatccaggac acgatgcggg cgatcgcgct
     3961 ctcgaccgac ggcaacgggc gctacgcgca cctcgacccg tacctcggcg gtgcgcatgc
     4021 ggtggccgag gccgcgcgca acgtcgcgtg cgccggggcg gtcccggtgg cgatcacgaa
     4081 ctgcctcaac ttcggcaacc ccgagcgccc cgaggtgata tgggccttcg ccgaggtcgt
     4141 ccgcgggatg ggcgacgcct gccgggcgct cggcacgccc gtcacgggcg gcaacgtctc
     4201 cttctacaac gagaccggag actccgcgat ctggcccacg ccggtcgtcg ggatgctcgg
     4261 tctgctcgac gactaccgct tcagcctgcg ccccgccttc ggaccggacc aggtcgtcta
     4321 cctgctcggc gagacccacg cggagctcgg cggctccgag tacgcggacg tggtcctcgg
     4381 gatggtgagc ggcctgccgc cggcgctcga tctcgaccgg gaagcggccc tgcaccgcgt
     4441 gctcgccgag ggggcccgaa ccgaggtgat cgcggcggct caggactgct ccgacggcgg
     4501 gctcgccgtc gcactcctcg aggcggcgat caacggggac accggcgtcg ccgcgaccct
     4561 tccgggcgac ctgcctccgc acgtcacgat gttctccgag tcctcctcgc gcgccgtcgt
     4621 cgccgtcgcg ccggagaagg ctgccgagct cgaggatctc gccgatcgac acgggctgcc
     4681 gtggacgcgg ctcggcgaca cgggcgggcc gcggatcgtg atcggtgagg tcgacctcac
     4741 ggtcgccgag gcccgccaga tctacgaggg cgccatcccc gccctcctgc agatgcacgc
     4801 ccaggccggc tgacggtccg aagcgatccc ccgcgccggg cgcgtcctcc cagcccgggg
     4861 aagggcctct gctaccatcc ccctgtggga ggcgagagtc cgaaggagga gtgcggcctc
     4921 ttcggggtct gggccccggg ggaggacgtc tcccggctca tctacttcgg cctctacgcc
     4981 ctccagcatc gcggccagga atcggccggg atggccatct cggacggctc gaacatgctc
     5041 gtctaccgcg agctcggcct cgtctcccag gtcttcaacg agcgcacgct ctcgacgctg
     5101 cagggcgacc tcggcatcgg ccacacccgc tactcgacca ccggctccac gacctgggag
     5161 aacgcccagc ccatcttcaa gaccgacggc ggacgcgcgc tcgcgctcgc ccacaacggc
     5221 aacctcgtga acacccagga tctcgccgac cgcgtcgggt tcctcggccg ctccacgagc
     5281 gacacggacc tcgtcgccac cctgctctcc cgggaggcgg aggacgggct cgaggcggcg
     5341 gcgctcaagg tgcttccgac cctgcagggg gcgtactgct tcctgatgag cgacgagaag
     5401 accgtctacg cggcccggga cccgctcggg atccggccgc tcgtgatcgg ccgcctcccg
     5461 aacgggttct gcttcgcctc ggagacggcg gcgctcgaca tcgtcggcgc cgcctacgtt
     5521 cgggacgtcg agccgggcga gctcgtgatg gtcgacgagc gcggtctgcg ctcggtccgc
     5581 ttcgccgaga gcccgcgcga ggccctctgc ctgttcgagt tcgtctacct cgcgcgagcg
     5641 gacacgacga tgatggggac gaccgttcat cgcgcgcgac gcgagatggg gaaggcgctc
     5701 gccgccgagg cgccggtgga agcggacctc gtgatcccga tccccgacac cgcgagctcg
     5761 gccgcccagg gctacgccga agcctccggg atcccgtacg gcgaaggtct gatgaagaac
     5821 cgctacgtcc accggacgtt catccagccg accccgggcc tgcggcagat gggggtccgg
     5881 ctgaagctca accccgtccc cgacgtgatc cggggacggc gcctcatcgt cgtcgacgac
     5941 tcgatcgtcc gggggaccac gactcgccag atcgtccagc tcctgcgcga ggcgggcgcg
     6001 accgaggtcc acatgcggat cacgagcccg ccggtccgct ggccctgctt ctacgggatc
     6061 gacatgtcga cccgccagga gctcgtcgcg tccgacctgt cggtcgacga ggtgcgcgag
     6121 ttcatcggcg ccgattcttt gagctatctc accctgccgt cgct
//
LOCUS       DATLGG010000057         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_160986, whole genome shotgun sequence.
ACCESSION   DATLGG010000057 DATLGG010000000
VERSION     DATLGG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160986"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1796
                     /locus_tag="VM638_01485"
     CDS             288..1796
                     /locus_tag="VM638_01485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVM11135.1"
                     /translation="MSQPSAADDCLWIATAGTTAYPALDRDLEAEVVVVGGGIVGLTA
                     ALLAAREGRRVVLLERDKVAMGVSPRATVKVSTGHGTLYSTIRSHFDDDRLRAYGEAN
                     LRGFRLIEELIGRHAIDCDWEPRDHFVWAEDAKQLEQVRDEVDAERIAGLASELVDAA
                     PELPFEVTGALRLPDQAQFHPRRYLLGLARAAEAAGVEIYERTPVSDVDGGASPVVDT
                     PSARVRGGEVILATHFPIVNRKMLFAEMTPKREYSVAGPVDPQRAPKHMYIGAGSPTR
                     SIRIAPSDDGPLLIVVGEGHKVGERVDTEGCYRALEAWGAEHFGVGRWTYRWSNQDNM
                     SMDGLPFVGRLEEGVSTATGLNAWGMTMGTVAAEMLASLAGGREDPLLGAFDPARTVH
                     APGEFLKQNIKVAGHFVGGRLAAFRGAVEEIAPGSADVVRIDGTPVAVYRDDGGALHA
                     VTATCTHMGCLVEWNAGDRSWDCPCHGSRFGLDGRVLNSPATEPLERIDPAD"
     gene            complement(1793..2575)
                     /locus_tag="VM638_01490"
     CDS             complement(1793..2575)
                     /locus_tag="VM638_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVM11136.1"
                     /translation="MATVETGRVLLSPEDYAVLGENDFGMAGLQATETIGPFVECQGS
                     GPLIMIHDAIVEARLGIGHHPHRLNERLFYILRGTLDHDDALNGITGHMGTNDLARLT
                     EGRRGMYHKEWNNGDEDTRAFILVYQTDPVPPTASFAALRDAEAPRYDESPGVRTKEL
                     VGPRSPLEINGDIRLYTDATFEADAALSFGFAEDESVLLYPLEGSFSVDDAKLEEGHT
                     LIVTPDARERRVEVRALSAGRLLRVVHGAGDGVEFGRPYRRG"
     gene            complement(2587..3033)
                     /locus_tag="VM638_01495"
     CDS             complement(2587..3033)
                     /locus_tag="VM638_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVM11137.1"
                     /translation="MQVKELGHIVLYVRNLERSRRFYGETLGWQEVARFGNQGAVFSS
                     GRTHHELLLLEVGEDATPIPAGRRVGMYHFGLKIGETDEELREALEHLLAAGVKVNGS
                     ADHTVTHSLYIEDPDGNEIELYIDVQPEIWREDPQAIVAPPRALRL"
     gene            complement(3100..>3492)
                     /locus_tag="VM638_01500"
     CDS             complement(3100..>3492)
                     /locus_tag="VM638_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11138.1"
                     /translation="IAEEAAKLVEVVAAQDLAELIKGFEQERGQEDKAIEGIAPTLRA
                     LAMAQVEVLLVDRTLGDERTAWFGPEAAHLSQRRDDVEGLGAAWVHEGPLVDACIRSA
                     LLSGARVAVVPPEAGIRDGLGALLRWST"
BASE COUNT          490 a         1218 c         1251 g          533 t
ORIGIN      
        1 ggtgccgtga gcgtcgggct ccgctcgttc cgcgggacgc ttcccgcggc tcccggcacc
       61 gagcgcgttt gggtgcgtgc ctgcctcggc gggtcctgcg ccacggcctc gttccgcctc
      121 ccgggcggct agcggctacc cgatgggttc agcgtcacga cggcgagcgc ggcgagcagc
      181 atctgcacag agggttccgc aagggtgcac ctgtggagga gaggccccga gcgatccgga
      241 tcccgctccg ggggattgac gggacagaag ccgggtaagg cggagccgtg agccagccca
      301 gcgccgccga cgactgcctg tggatcgcga ccgcggggac gacggcgtat cccgctctgg
      361 accgcgacct cgaggccgag gtcgtggtcg tcggaggcgg gatcgtcggg ttgacggccg
      421 cgctgctggc ggcgcgtgag gggcgccgtg tcgtcctgct cgagcgggac aaggtcgcga
      481 tgggggtctc ccctcgagcc acggtcaagg tctcgaccgg ccacgggacc ctctactcga
      541 cgatccggag ccacttcgac gacgaccggc tgcgggccta cggcgaggcc aacctgcgcg
      601 ggttccgcct gatcgaggag ctgatcggcc ggcacgcgat cgactgcgac tgggagccgc
      661 gggatcactt cgtgtgggcg gaggacgcga agcagctcga gcaggttcgc gacgaggtcg
      721 acgcggagcg catcgcgggc ctcgccagcg agctcgtcga tgcggcgccg gagctaccct
      781 tcgaggtcac gggggctctg cggttgccgg atcaggcgca gttccaccct cgccgctacc
      841 tcctcgggtt ggctcgtgcc gccgaggccg cgggggtgga gatctacgaa cgcacccccg
      901 tcagcgacgt ggacggggga gcgtccccgg tggtggacac gccgtcggct cgggtccggg
      961 gtggagaggt gatcctcgcc acccacttcc cgatcgtgaa ccggaagatg ctgttcgctg
     1021 agatgacgcc gaagcgtgag tactcggtcg cgggccccgt ggatccgcag cgtgcgccga
     1081 agcacatgta catcggcgcc ggatccccca cgcggtcgat ccggatcgcc ccgtcggacg
     1141 acgggccgct gctcatcgtc gtcggcgaag ggcacaaggt gggcgagcgc gtcgacaccg
     1201 agggctgcta ccgggcgctc gaagcctggg gggcggagca tttcggcgtc ggtcggtgga
     1261 cctatcgctg gtcgaaccag gacaacatgt cgatggacgg gctcccgttc gtcgggcgct
     1321 tggaggaggg ggtctcgacc gcgaccgggc tcaacgcgtg ggggatgacc atgggaacgg
     1381 tcgccgccga gatgctcgcg tccctggccg gcgggcgcga ggatcccctg ctcggcgcct
     1441 tcgatcccgc ccgcacggtg catgctcccg gtgagttcct gaagcagaac atcaaggtcg
     1501 cgggtcactt cgtcggcgga cgcctcgcgg ccttccgcgg ggccgtcgag gagatcgcac
     1561 cgggatccgc cgacgtggtc cggatcgatg gaacgccggt ggccgtctat cgcgacgacg
     1621 ggggcgcgct ccacgcagtc accgcgacct gcacgcacat gggttgcctc gtcgagtgga
     1681 acgcggggga tcgcagctgg gattgcccgt gtcacgggtc ccggttcggg ttggacggcc
     1741 gcgtgctgaa ctcaccagcg accgaaccgc tggagcggat cgacccggcg gactagcccc
     1801 ggcggtacgg gcgcccgaac tcgacgccat cgccggcgcc gtgcacgacg cgcaagaggc
     1861 gcccggcgga gagcgcccgc acctcgaccc gccgttcgcg ggcgtcggga gtgacgatca
     1921 gggtgtgtcc ctcctcgagc ttcgcgtcgt cgaccgagaa ggatccctcg agcgggtaga
     1981 gcaacaccga ctcgtcctcg gcgaacccga aggagagcgc ggcgtcggcc tcgaaggtgg
     2041 cgtcggtgta gaggcggatg tcgccgttga tctcgagcgg gctgcgggga ccgaccagct
     2101 ccttcgtccg cacccccggc gactcgtcgt accgcggagc ctccgcatcg cgcagggccg
     2161 cgaaggacgc ggtcggcgga acggggtcgg tctgatacac gaggatgaag gcccgcgtgt
     2221 cctcgtcccc gttgttccac tccttgtggt acatcccgcg ccggccttcg gtcagccgtg
     2281 cgaggtcgtt cgttcccatg tggcccgtga tgccgttcag cgcgtcgtca tggtcgaggg
     2341 tcccgcggag gatgtagaag agccgctcgt tcaggcggtg agggtggtgg ccgatcccga
     2401 ggcgcgcctc gacgatcgcg tcgtggatca tgatcagggg accggagccc tggcactcga
     2461 cgaacggccc gatcgtctcc gtcgcctgca ggccggccat cccgaagtcg ttctccccga
     2521 ggaccgcgta gtcctcgggg gagaggagca cccgtcccgt ctcgaccgtc gccatcggta
     2581 cctccctcag agccgaagcg cccgcggcgg cgcgacgatc gcctgcgggt cctcgcgcca
     2641 gatctccggc tggacgtcga tgtagagctc gatctcgttg ccgtccggat cctcgatgta
     2701 gaggctgtgc gtgaccgtgt ggtcggcgga accgttcacc ttgaccccag ccgcgaggag
     2761 gtgctcgagc gcctcgcgga gctcctcgtc ggtctcgccg atcttcagcc cgaagtggta
     2821 catgcccacg cgcctccccg ctgggatcgg cgttgcatcc tcgccgacct cgaggagcag
     2881 cagctcgtga tgcgtgcggc ccgacgagaa gacggcgccc tggttcccga agcgggccac
     2941 ctcctgccac ccgagcgtct cgccgtagaa ccgtcgcgag cgctcgaggt tgcgcacgta
     3001 caggacgatg tgtccgagct ccttcacctg catcgctgcc cccgtccggc cgtcatcgtc
     3061 cccatcatct cgcggatcga cgggaacgtc cgcgccggat caggtgctcc agcgcaggag
     3121 ggctccgagg ccgtcgcgga tcccggcctc cggcggaacg acggcgaccc gcgccccgct
     3181 gagcaacgcc gagcggatgc aagcgtccac gagcggcccc tcgtgcaccc aggccgcgcc
     3241 gagcccctcc acgtcgtcgc gccgctgtga caggtgcgcc gcctcgggac cgaaccacgc
     3301 ggttcgctcg tcgccgagcg tccgatcgac gagcagcacc tcgacctggg ccatggccag
     3361 agcccgcagc gtgggggcga tcccttcgat cgccttgtcc tcctggcccc gctcctgctc
     3421 gaagcccttg atcagctcgg ccaggtcctg cgccgccacc acctcgacga gcttcgccgc
     3481 ctcctccgcg at
//
LOCUS       DATLGG010000058         5525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_192242, whole genome shotgun sequence.
ACCESSION   DATLGG010000058 DATLGG010000000
VERSION     DATLGG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192242"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VM638_01505"
     CDS             <1..723
                     /locus_tag="VM638_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVM11139.1"
                     /translation="EQRNALSEEMLGDLAAALEEAAAASEARVVLVTGEGPDFCAGAD
                     LADIQAAAEAGEGAYADTYGQALDRALDAIASHPLPVVAAVQGAALGGGCQLAVAADL
                     TLAAADARLGLPLLRLGVVVSYPNVVRLTASVGWKRASAMLLAGRVISGEEAAAWGLV
                     SEAVPRADLTARAETVAGQIAAGAPISVRAAKRGLQAALEQSEGDRGVFEMMLAQAFA
                     SEDLREGIRAFRERRAPRFEGR"
     gene            720..1463
                     /gene="nth"
                     /locus_tag="VM638_01510"
     CDS             720..1463
                     /gene="nth"
                     /locus_tag="VM638_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVM11140.1"
                     /translation="MSARPAREAPREDRAPEILRLLKREYRGATMALRYSSPLECLVA
                     VILSAQCTDARVNEVTRGLFQKYRSAEDYLAVPESELAADIRPTGFFNQKAKSIRGTC
                     RMIVEEYGGRVPDTMADLIRLPGVARKTANIVQGNVYPEKAARDPDKGVAVDTHVKRL
                     SNRLGFSAESDPSKIERDLMDLLPRKEWFPITYVLIEHGRAVCKAPKPRCEECVVERL
                     CPASRLAASGDPGGRAATPGEARPRRRRA"
     gene            complement(1396..1986)
                     /locus_tag="VM638_01515"
     CDS             complement(1396..1986)
                     /locus_tag="VM638_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HVM11141.1"
                     /translation="MTALAQALPRPRSRPAALASDVALIVGGSLFVAALAQVSVPLPF
                     TPVPISGQTLGVLLVGTSFGWSRGALTLALYLLWGAIGLPFYAEAKAGAAFLGPESPT
                     GGYLWGFLLAATLTGWLANRGWDRRFVTAVAVMVAGNVLIYLLGVPWLAQAIDVPLRE
                     AMVLGLFPFLIGDALKLVVAAGALPLAWRLVPRDRR"
     gene            complement(1988..2251)
                     /locus_tag="VM638_01520"
     CDS             complement(1988..2251)
                     /locus_tag="VM638_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVM11142.1"
                     /translation="MDWVHRARCKEEDPELFFPVGTTGPAADQIEAARAVCLRCEVKE
                     ECLEWALTTGQDAGVWGATSEDERRAIRRARRREAAATRLAVG"
     gene            2385..3146
                     /locus_tag="VM638_01525"
     CDS             2385..3146
                     /locus_tag="VM638_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVM11143.1"
                     /translation="MSDLRPPLVLAHRGASAREPENTLRAFRAAEVDGADMIELDVRL
                     TADGVPVVIHDDDLARTTDGTGRVHRTRLDELKRLDASGGRGEPEPVPTLAEALAATT
                     VALDVELKDLPGEPSFDWPETRLVEATLEVLSAARPERAILLSSFDWLALERVRALAP
                     DLETGFLTIGGVDPLAAVRYAAGAGHRWILPQAPALLEAGGVAVERAHEAGLRVGVWT
                     VDAPDLMQRLFAWGVDALATNEPAVAVEVRDSVRE"
     gene            complement(3132..4040)
                     /locus_tag="VM638_01530"
     CDS             complement(3132..4040)
                     /locus_tag="VM638_01530"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVM11144.1"
                     /translation="MRLLLIANVEAQAVTPRAAAVVLRTLAADLEVDLIHTTHPGHAT
                     DLAREARGTVEMVVAMGGDGTVNEVANGLAGSDVTLGIVPGGGANVFARSLGIPRDPV
                     EATRLLVARRERRGRRVTLGRALGRHFLFACGIGLDGAVVRRVERRRPVRKALGQLSY
                     VWAGLTQIARFDRRHPKLAVRWGASLEHRRSRIFVAIAQNTDPFTYLGALPMRLSPTA
                     GLETGLDLVGLARAGIPTILGVAAGAFRGRGRHLERDAVLHVRDQARIEVRSEVPLPV
                     QMDGEYVGDHELLELEAVPGALSLAY"
     gene            4084..4401
                     /locus_tag="VM638_01535"
     CDS             4084..4401
                     /locus_tag="VM638_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11145.1"
                     /translation="MFRRRSALPDPFVRVAEHVEAAKEALVGAMPTARRPGTPLAEAI
                     HRFEEEVAAARSALAAWDDPGHEMERLRLEAPALDFEGMAIVLGDLIAPLDVFQEAEH
                     RLR"
     gene            4463..>5525
                     /locus_tag="VM638_01540"
     CDS             4463..>5525
                     /locus_tag="VM638_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVM11146.1"
                     /translation="MGTINLVTDIPGPKSRAITERKERVVAEPLDLHAPVVVDTARGA
                     SFTDVDGNTFLDFSGGIGCHTVGYSHPKVVEAAVEASRRFSHTDFTIIPYESYVEVSE
                     RLVEKVGGDRKVALFNSGAEAVENAVKIARAATGRQAVIAFEGAFHGRTLMAMSLTSR
                     HSPYKTKFGPFAPEVYRAPFPYPYRSPDPENAGRLALEALERAFSTQVDPDNTAAVIV
                     EPVQGEGGFVVPTPDFLPGLAEICKRHGVLLIVDEIQTGFGRTGRFFAYEHAGIDPDL
                     VCVAKSIAGGYPLSGVVGRTDVMDALGPSTIGGTYVGNPVACAAATAVFEVIEEEGLV
                     ERATHVAKTIRDRWTELASS"
BASE COUNT          801 a         1976 c         2030 g          718 t
ORIGIN      
        1 gagcagagga acgcgctctc ggaggagatg ctgggcgacc tcgccgccgc cctggaggag
       61 gccgccgccg cgtcggaagc gcgggtcgtc ctcgtcaccg gggaggggcc ggacttctgc
      121 gccggcgccg acctggccga catccaggcc gccgcggagg cgggcgaggg ggcctacgcc
      181 gacacgtacg gtcaggcgct cgaccgggcg ctggacgcga tcgcgtcgca ccccctgccg
      241 gtcgtggccg cggtgcaggg cgccgcgctc ggtggaggat gccagctcgc ggtagccgcc
      301 gacctgacgc tcgcggctgc cgacgcgcga ctcggtctgc cgctcctccg gctcggggtc
      361 gtcgtgagct atccgaacgt cgtccgcctc acggcgtcgg tcggctggaa gcgtgcctcg
      421 gcgatgctgc tggccggccg cgtgatatcg ggcgaggagg ccgcggcatg ggggctcgtg
      481 agcgaggcgg tcccgcgggc cgacctcacc gcgcgtgccg agacggtcgc cggacagatc
      541 gccgcgggcg ccccgatctc ggtgcgagcc gcgaaacgcg gcctgcaggc ggcgctcgag
      601 cagagcgaag gcgaccgtgg cgtcttcgag atgatgctcg cccaggcgtt cgccagcgag
      661 gacctccgtg agggcatccg cgcgttccgc gaacgtcgcg caccgaggtt cgagggccga
      721 tgagcgcgcg tccggcgcga gaagccccgc gggaggatcg ggcacccgag atcctccgcc
      781 tcctgaagcg cgagtaccgc ggggccacga tggcactgcg gtactcgtcc ccgctcgagt
      841 gcctcgtggc cgtcatcctc tcggcgcagt gcaccgatgc gcgggtgaac gaggtcacgc
      901 ggggcctctt ccagaagtac cgctccgcgg aggactacct cgcggtgccg gagtcggagc
      961 tcgcggccga catccgcccc accggcttct tcaaccagaa ggcgaagtcg atccgcggca
     1021 cctgccggat gatcgtcgag gagtacggcg gccgcgtgcc ggacaccatg gcagatctga
     1081 tccggcttcc cggggtcgcg cggaagacgg cgaacatcgt gcaggggaac gtctatccgg
     1141 agaaggcggc acgcgacccg gataaggggg tcgccgtcga cacgcacgtc aagcgcctgt
     1201 cgaaccgcct cgggttctcc gccgagtccg acccgagcaa gatcgagcgc gacctcatgg
     1261 acctgctgcc tcgcaaggag tggttcccga tcacgtacgt gttgatcgag cacggccgcg
     1321 ccgtctgcaa ggccccgaag ccacgctgcg aggagtgcgt cgtcgagcgg ctgtgtccgg
     1381 cgagcaggct cgcagctagc ggcgatcccg ggggacgagc cgccacgcca ggggaagcgc
     1441 gcccgcggcg acgacgagct tgagcgcatc cccgatcagg aacgggaaca gcccgagcac
     1501 catcgcttcc cgaagcggaa cgtcgatcgc ctgcgcgagc cacgggacgc ccaggaggta
     1561 gatcaacacg ttgccggcga ccatgaccgc caccgccgtc acgaagcggc ggtcccatcc
     1621 gcggttcgcc agccagccgg taagcgtcgc ggcgaggagg aagccccaga ggtagccgcc
     1681 ggtcggcgac tccggtccga ggaaggcggc tcccgccttc gcctcggcgt agaacggcag
     1741 cccgatcgcc ccccagagca ggtagagcgc gagggtcagg gcgccccgcg accagccgaa
     1801 ggacgtcccg acgagcagca cgccgagcgt ctgaccgctg atcggcaccg gtgtgaacgg
     1861 cagggggacc gagacctgcg ccagcgcggc cacgaagagg ctcccgccca cgatcagcgc
     1921 gacgtcggac gcgagcgcgg ccggccgaga gcgtggccgg gggagcgcct gggcgagggc
     1981 ggtcatctca gccgacggcg agtcgggtgg cggcggcctc tcggcggcgg gcacgccgga
     2041 tcgcgcgacg ctcgtcctcg gaggtcgccc cccagacgcc ggcatcctgg ccggtggtga
     2101 gcgcccactc gaggcactcc tccttgacct cgcatcggag gcacacggcg cgggcggctt
     2161 cgatctggtc agcggcggga ccggtggtcc cgacggggaa gaagagctcg gggtcctctt
     2221 ccttgcaacg tgctcggtgt acccagtcca tcaggaccct ccttcggagg accgccgctt
     2281 gtgaaatcgt tcacaagatc ctccggtcga accgcgccaa cgtcgacgcg gaaggccgag
     2341 tatagtggcc gcctcccgcc acgtgtcaag acccaccttc gcagatgagc gacctccggc
     2401 ctcccctcgt gctcgcgcat cgcggcgcct ccgcgcgcga gccggagaac accctccggg
     2461 cgttccgagc cgcggaggtc gatggggccg acatgatcga gctggacgtg cgcctcaccg
     2521 ccgacggcgt gccggtcgtg atccacgacg acgacctcgc ccgcacgacc gacggcaccg
     2581 ggcgcgtcca ccgcacccgc ctcgacgagc tgaagcggct cgatgcgtcg gggggtcgcg
     2641 gggagcccga gccggtgccg accctcgccg aggccctcgc agccacgacg gtcgcgctgg
     2701 acgtggagct caaggacctc cccggcgagc ccagcttcga ctggcccgag acgcggctcg
     2761 tggaggcgac gctcgaggtc ctgtctgcgg ctcggcccga gcgagcgatc ctcctctcgt
     2821 ccttcgactg gctcgcgctg gagcgcgtgc gcgcgctcgc acctgacctg gagacggggt
     2881 tcctcacgat cggaggggtg gacccgctgg cggcggtccg ctacgcggcc ggggcggggc
     2941 accgctggat cctcccgcag gcgccggcgc tcttggaggc cggcggggtg gctgtcgagc
     3001 gtgcccacga ggcgggcctg cgggtcggcg tctggaccgt cgacgcgccc gacctcatgc
     3061 agcggctctt cgcctggggc gtggatgcgc tcgcgacgaa cgagccggcc gtggcggtcg
     3121 aggtccgcga ttcagtacgc gagtgacagg gcgcccggga cggcctcgag ctcgagcagc
     3181 tcgtggtcgc cgacgtactc cccgtccatc tggaccggga gcgggacttc gctgcgcacc
     3241 tcgatccgag cctggtcgcg gacgtggagc acggcgtcgc gttcgaggtg gcggccgcgc
     3301 cctcggaatg cccccgcggc gacgcccagg atcgtcggga tccccgcgcg agccaggccg
     3361 acgaggtcga ggcctgtctc cagcccggcg gtcggggaga gacgcatcgg gagcgcgccg
     3421 aggtacgtga aggggtcggt gttctgcgcg atcgctacga agatcctcga gcggcggtgc
     3481 tcgagggagg cgccccagcg caccgcgagc ttcgggtggc ggcgatcgaa ccgggcgatc
     3541 tgcgtcaacc ccgcccagac gtaggagagc tgccccagag ccttccgtac ggggcgccga
     3601 cgctcgacgc ggcggacgac cgcgccgtcg agcccgatcc cgcaggcgaa caggaaatgg
     3661 cgtcccagcg cgcgcccgag cgtgacgcgt cggccgcgac gttcgcggcg ggccacgagc
     3721 agccgggtcg cctcgaccgg gtcccgcggg atcccgagcg agcgcgcgaa gacgttcgcc
     3781 ccgccgccgg ggacgatccc gagggtcacg tcgctcccgg cgaggccgtt cgccacctcg
     3841 ttcacggtcc cgtcgccccc catcgccacg accatctcga ccgtccctcg ggcctcacgc
     3901 gcgaggtccg tggcgtgccc cgggtgcgtc gtgtggatga ggtcgacctc gaggtcggcg
     3961 gcgagggtcc gtaggacgac ggccgcggca cgcggggtga cggcctgtgc ctcgacgttc
     4021 gcgatcaaca ggagacgcac ggccgctgat tctggcagac ccggcggtcc gtacgatacg
     4081 gggatgttcc gtcgccgctc cgccctgccc gacccgttcg tccgcgtcgc cgagcacgtc
     4141 gaggcggcga aggaggcgct ggtcggcgcg atgccgaccg cccggcgtcc cggcaccccg
     4201 ctcgccgaag cgatccaccg gttcgaggag gaggtcgccg cggctcgctc cgcgctcgcg
     4261 gcctgggacg accccggcca cgagatggag cgcctgcgct tggaggcgcc cgccctggac
     4321 ttcgagggga tggcgatcgt cctcggcgac ctgatcgcgc cgctcgacgt cttccaggag
     4381 gccgaacacc ggctccgctg accgccggcg ccgatgcgcg cggtgctacc ctctgtcgga
     4441 cgtcgaacga aggagcgagc gcgtgggcac catcaacctg gtcaccgaca tccccggccc
     4501 gaagtcgcgg gcgatcaccg aacggaagga gcgcgtggtc gccgaaccgc ttgacctgca
     4561 cgcgcccgtg gtggtcgaca ccgcccgcgg cgcctcgttc accgacgtcg acggcaacac
     4621 gttcctcgac ttctcgggcg ggatcggctg ccacaccgtc gggtactcgc atccgaaggt
     4681 cgtcgaggcc gcggtcgagg cgtcgcggcg cttcagccac accgacttca cgatcatccc
     4741 gtacgagagc tacgtcgagg tgagcgagcg cctggtcgag aaggtcgggg gcgaccggaa
     4801 ggtggcgctg ttcaactccg gcgccgaggc cgtggagaac gcggtgaaga tcgcgcgtgc
     4861 cgcgaccgga cgccaggcgg tgatcgcgtt cgagggggcc ttccacggcc ggacgctcat
     4921 ggcgatgtcg ctgacgagcc ggcacagccc ctacaagacg aagttcggac cgttcgcgcc
     4981 ggaggtctac cgggccccgt tcccgtaccc gtaccgcagc cccgatccgg agaacgccgg
     5041 gcggctggcc ctggaggcgc tcgagcgcgc cttctcgacc caggtggacc ccgacaacac
     5101 ggcggccgtg atcgtcgagc ccgtgcaggg ggaggggggc ttcgtcgtcc cgaccccgga
     5161 cttcctcccc gggctcgccg agatctgcaa gcggcacggc gtcctcctca tcgtcgacga
     5221 gatccagacg ggcttcggac ggaccggccg gttcttcgcc tacgagcacg ccggcatcga
     5281 cccggatctc gtgtgcgtgg cgaagtccat cgccggcggg tatccgctct ccggcgtcgt
     5341 cgggcggacc gacgtgatgg acgccctcgg gccgtcgacg atcggcggga cctacgtcgg
     5401 gaaccccgtc gcgtgcgcgg cggcaacggc ggtcttcgag gtgatcgagg aggagggact
     5461 cgtcgagcgt gccacccacg tggcgaagac gatccgcgac cggtggaccg agctcgcctc
     5521 gagcg
//
LOCUS       DATLGG010000059         6198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_192271, whole genome shotgun sequence.
ACCESSION   DATLGG010000059 DATLGG010000000
VERSION     DATLGG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192271"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VM638_01545"
     CDS             <1..761
                     /locus_tag="VM638_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVM11147.1"
                     /translation="IGARAAVALGRRGARLVLAARREDRLREIANRIRDRGGPGALVV
                     PTDVTKVGDLRALAERVRTEAGRCDVLVNNAGIPGGGAFADLSLAHLERVTATNYLSV
                     LRLTKLLLPMLADAGGHVVNVASLAGRFAVPGAAAYTASKHAVVALSEALYHELAPRG
                     VMVTSVNPGFVHTEGFPHRELQQDPRLRRFVMGPSPTVRAIVEVIEGRRGPEVSVPRW
                     IGPLQAFRILTPPLYRAAVRRLSGRRTAPPDPSA"
     gene            complement(751..1131)
                     /locus_tag="VM638_01550"
     CDS             complement(751..1131)
                     /locus_tag="VM638_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11148.1"
                     /translation="MTAMSGPGATGQRPPLVLVAAILLFVAGVLNLLAAFSAMQLGGA
                     GAIYLLLYLALGAGAIFAGIQILNGREQGRMIGMAVAGVGLVLQVLALIAFRGLGAAY
                     IIIGILLYGVILYALATTRTHFTR"
     gene            complement(1185..2594)
                     /gene="pyk"
                     /locus_tag="VM638_01555"
     CDS             complement(1185..2594)
                     /gene="pyk"
                     /locus_tag="VM638_01555"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HVM11149.1"
                     /translation="MADRSRATKLVCTLGPAVEDRLDDLVAVGLDVARINLSHGTRET
                     HERLLQRVRKASAAAGRTVGVLVDLSGPKVRLGEVAEGRVGLAEGARFELRPGGGPGD
                     LRGAPTSHEGLADDLRVGDRVLLADGAAELQVTGVDGDVVVTTVTSGGIVGSRSGINI
                     PAERLSLPAITARDERDIAWAREAGVDMVGQSFVRWPDDVLALRDLLGEDAPLVVAKV
                     ETGPAVRHAEGIIEAADGLMVARGDLGVELPLEEIPLIQRDLVFRCVQAGKPVIVATQ
                     MLESMREAPRPTRAEASDVAGAVFDGADAIMLSAETAVGAYPIEAARTAARIAEVAGG
                     SGYVAAPPRLPAPTDAHLVAQAALSVSLQGGMTAIACFTQSGMTAVLIAATRPRVPVY
                     AFSPEVTVVRRLTLYRGVTPFLTDPLHDTDAMIATVDGRLQEGGFVRPGDAVLMVGSS
                     PFGEANTNLLKIHRIGSSR"
     gene            complement(2587..3708)
                     /locus_tag="VM638_01560"
     CDS             complement(2587..3708)
                     /locus_tag="VM638_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HVM11150.1"
                     /translation="MSDRARFVFSEDLREYDHGPQHPLRPVRVLLTVDLIASLGLLER
                     ANVERAPARVASDEELMLVHTEPYLDAVKRSGEASRADLVRYGFGPGDNPIFPNMHRA
                     GARVAGATLVAAEAVVRDGFDHAFNPAGGLHHAMPDRASGFCVYDDPAVAIAWMLEQG
                     VERVAYVDVDVHHGDGPQAIFYEDPRVLTISLHESGRYLFPGTGFASERGAGPGEGTA
                     VNVPLPPTTGDDAWLEAFRAVVPPLVRRFAPQVLVSQLGCDTHHTDPLAHLGLTTRAY
                     RETARALHDLAHEVCDGRWIATGGGGYRWASVVPRAWTIAFAEMAGAELPDEIPADWL
                     RRAEEAGEGRVPATFSEAAPERADPPIDGILEEVLRGRG"
     gene            complement(3761..5206)
                     /locus_tag="VM638_01565"
     CDS             complement(3761..5206)
                     /locus_tag="VM638_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HVM11151.1"
                     /translation="MTEAVSHRWKSIDDLPEDVRTLTNPDLAQLASSWSAQQSALRGT
                     AELGEFEQQLRNEWAVETGIVEGLYALPRPVSERLIREGVDASILAASGVKEPELLAG
                     MVADQLFALRRIEQMAADPRGISTSDIKELHALITLHLAGDGGPMLKGAYKLGPNDVT
                     RPDGSSHQFCPPEQVAWEMERLLELHEKHQARDLPADVQAAWLHHGFMQIAPFQAGNG
                     LIARALATAIFVKNGWFPLIVRRDDLERYAAAQDRADAGDLKGLVDLFAFIQRRTLLE
                     APGAVGDSLRKERVDQVISSAKAVLEQRQRADDVGWAEAAEIAKSLLVVAEERLRRTA
                     DRMSQDLRQLAPDFRFEVDTEKSGGPRDYFFKWHIIESARQLDYFANLDIYRSWTRLM
                     MRTATQDEILLSFHAVGDQSDGKIVATMCFYRRDRVQRGEENLADLSTVCDQLFEVDP
                     NETAEGARERFTPWLEAGLVRGLELWHESLG"
     gene            complement(5296..>6198)
                     /locus_tag="VM638_01570"
     CDS             complement(5296..>6198)
                     /locus_tag="VM638_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM11152.1"
                     /translation="GLPPGVLNLVHGYGDEAGAPIVAHDDVRGISFTGSLEVGRMINE
                     QCGRLMKRCSLELGSKNVLIVMPDAQMDLAVEAAAWGAFATSGQRCTATSRLVVHDAA
                     RQEFTERLLERVGGMKVGSGLEPDVELAPVINAKQKQRVLEYIEIGKAEGAKVLTGGE
                     ELTGNGYENGNFIAPTVFDEMTPDMRIAQEEIFGPVTGIIRTDDVEEAIRIANSVQYG
                     LSASIYTHDITNVFKAVQQLEFGIVYVNAPTIGAEIQLPFGGMKNTGNGHREAGPQSL
                     DEFMEWKSVSVDFSGKVQKAQGID"
BASE COUNT          883 a         2269 c         2166 g          880 t
ORIGIN      
        1 ggatcggcgc gcgcgccgcg gtggcgctcg gacgccgggg cgcccgcctc gtgctcgccg
       61 cccgccggga ggatcgcctg cgcgagatcg ccaaccggat cagggatcgc ggcggtcccg
      121 gggcgctcgt cgtcccaacc gacgtcacga aggtcggcga cctccgggcc ctcgccgagc
      181 gggtgcgcac ggaggccggc cgctgcgatg tgctcgtcaa caacgcaggg atccccggcg
      241 gtggcgcgtt cgcggacctc tctctcgcgc acctcgagcg ggtgacggcg acgaactacc
      301 tgtcggtcct tcgcctgacg aagctcctgc tcccgatgct ggccgacgcc gggggtcacg
      361 tcgtcaacgt cgcatcgctc gccgggcgct tcgccgtccc gggcgccgcc gcctacacgg
      421 cctcgaagca cgcggtcgtc gcattgtcgg aggcgctgta ccacgagctc gcgccccgcg
      481 gggtgatggt gacgtcggtg aacccggggt tcgtgcacac cgaggggttc ccccaccgcg
      541 agctccagca ggatccgcgg ctccgccgct tcgtcatggg tccctcgccg acggtgcgcg
      601 ccatcgtcga ggtgatcgag ggacggcgcg ggcccgaggt gtcggtgccc cgctggatcg
      661 gaccgctgca ggcgttccgc atcctgaccc cgccgctcta ccgagcggcg gtccgccggc
      721 tcagcggccg gcggaccgct ccgcccgacc ctagcgcgtg aagtgcgtgc gagtcgtcgc
      781 gagcgcgtac aggatgaccc cgtacagcag gatcccgatg atgatgtacg cggccccgag
      841 cccacggaag gcgatcagcg ccagcacctg gagcacgaga cccacgccgg cgacggccat
      901 gccgatcatg cgcccctgct cccgcccatt gaggatctgg atgccggcga agatggcccc
      961 ggcgcccagc gcgaggtaca gcaggaggta gatcgcgccc gcgcccccca gctgcatcgc
     1021 ggagaaggcg gcgagcaggt tcaggacacc cgcgacgaac agcaggatcg cggcgacgag
     1081 gacgagcggc ggacgctggc ccgtggctcc gggtccggac atggctgtca tgcattgctc
     1141 ctttcggtcg gccgcgaaac tctacggccg ggcgaagcaa ggagtcaacg cgagctcccg
     1201 atgcggtgga tcttcagcag gttcgtgttc gcctccccga agggggacga accgaccatc
     1261 aggacggcat ccccgggccg gacgaacccc ccctcctgga gccgcccatc gacggtcgcg
     1321 atcatcgcgt ccgtgtcgtg gagcgggtcg gtgaggaagg gcgtgacccc gcggtacagc
     1381 gtgagccgcc gaacgaccgt cacctccggg gagaaggcgt agacgggcac gcgggggcgc
     1441 gtggccgcga tcagcacggc ggtcatcccg gactgggtga agcacgcgat cgcggtcatc
     1501 ccgccctgga gcgagaccga cagcgccgcc tgtgcgacca ggtgcgcgtc ggtcggcgcg
     1561 gggagcctcg gcggcgcggc gacgtacccc gagccgcccg cgacctcggc gatgcgcgcg
     1621 gccgtccgcg ccgcctcgat gggataggcc cccacggcgg tctcggccga gagcatgatc
     1681 gcgtccgcgc cgtcgaacac cgcgcccgcg acgtcgctcg cctccgcccg ggtgggcctc
     1741 ggcgcctcgc gcatcgactc gagcatctgc gtcgccacga tcaccggctt cccggcctgg
     1801 acgcaccgga agacgaggtc gcgctggatc agggggatct cctcgagcgg gagctcgacc
     1861 ccgaggtcgc cgcgcgcgac catgagcccg tccgccgcct cgatgatccc ctccgcatga
     1921 cgcacggccg gaccggtctc caccttcgcc acgacgagcg gtgcgtcctc gccgaggaga
     1981 tcgcggagcg cgaggacgtc gtcgggccag cggacgaacg actggccgac catgtccacc
     2041 ccggcctcgc gagcccacgc gatgtcccgc tcgtccctgg ccgtgatcgc cggcaggctc
     2101 aggcgctccg ccgggatgtt gatcccgctc cgcgacccga cgatcccgcc actcgtcacg
     2161 gtcgttacga cgacgtcgcc gtcgaccccc gtcacctgga gctccgcggc cccgtcggcg
     2221 agcaggaccc ggtcgcccac ccgaaggtcg tcggcgagcc cctcgtggct cgtcggagca
     2281 ccccgcaggt ctcccggtcc gccgcccggc cgaagctcga acctcgcacc ctcggccaga
     2341 ccgacccgcc cctccgccac ctcgccgaga cggaccttcg gccccgacag gtcgaccagg
     2401 acgccgacgg tccgccccgc ggccgccgag gccttccgga cgcgttggag caggcgctcg
     2461 tgcgtctcgc gggttccgtg cgagaggttg atcctcgcca cgtcgagccc aaccgccacg
     2521 agatcatcca ggcggtcctc cacggcaggc ccgagcgtgc agaccagctt cgtggcgcgc
     2581 gaacgatcag ccacgcccgc ggagcacctc ctcgaggatg ccgtcgatcg gcgggtccgc
     2641 tcgctccgga gcggcctcgc tgaacgtcgc cgggacccgc ccctccccgg cctcctcggc
     2701 acgtcgcagc cagtcggcag ggatctcgtc ggggagctcg gcgcccgcca tctcggcgaa
     2761 cgcgatcgtc cacgcacgcg gcacgacgct ggcccagcgg tagcccccgc cgccggtcgc
     2821 gatccagcga ccatcgcaga cctcgtgcgc gaggtcgtgc aacgcccgcg cggtctcgcg
     2881 gtaggcccgc gtggtcaagc cgaggtgcgc gagggggtcg gtgtgatggg tgtcgcaccc
     2941 gagctggctc acgaggacct gcggcgcgaa gcgtcgcacg agcgggggca cgaccgcgcg
     3001 gaaggcctcc agccacgcat cgtccccggt cgtgggcggc agcgggacgt tcacggccgt
     3061 cccctccccc ggcccggcac cgcgctcgct cgcgaagccg gtgccgggga acaggtaccg
     3121 gccggactcg tggagcgaga tcgtcagcac gcgcgggtcc tcgtagaaga tcgcctgcgg
     3181 gccgtcgccg tggtggacgt cgacatcgac gtaggcgacc cgctcgaccc cctgctcgag
     3241 catccaggcg atggccaccg cggggtcgtc gtacacgcag aaccccgagg cccggtcggg
     3301 catcgcgtgg tgcaggccac ccgccgggtt gaacgcgtga tcgaacccgt cgcggaccac
     3361 cgcctcggcc gcgacgagcg tggcgccggc gacgcgcgcc cccgcccggt gcatgttcgg
     3421 gaagatcggg ttgtccccgg ggccgaagcc gtagcgcacg aggtcagcgc gggaggcctc
     3481 gccggagcgc ttcaccgcgt cgaggtacgg ctcagtgtgg accagcatga gctcctcgtc
     3541 gctcgcgacc cgcgccggcg cgcgttcgac gttcgcgcgc tcgaggagcc cgagggacgc
     3601 gatcaggtcg acggtcagca gcacgcgcac cgggcggagg ggatgctgcg gaccgtggtc
     3661 gtactcgcgc aggtcctccg agaacacgaa ccgagcccgg tcgctcacgc agcaggcacg
     3721 ggggacggca tcgcggagag cctaccccgc gcggaaaacg ctacccgagg gactcgtgcc
     3781 agagctcgag gccccgcacg aggcccgcct cgagccaggg ggtgaagcgc tcgcgcgccc
     3841 cctccgccgt ctcgttcggg tcgacctcga agagctggtc gcacaccgtc gagaggtcgg
     3901 cgaggttctc ctcgccacgc tggacgcggt cccgccggta gaagcacatc gtcgcgacga
     3961 tcttcccgtc cgactggtcg cccaccgcgt ggaaggagag caggatctcg tcctgggtcg
     4021 ccgtccgcat catgagccgc gtccacgacc ggtagatgtc gaggttcgcg aagtagtcca
     4081 gctggcgcgc gctctcgatg atgtgccact tgaagaagta atcgcgcggg ccgccgctct
     4141 tctcggtgtc gacctcgaag cggaagtcgg gggcgagctg ccgcaggtcc tgcgacatcc
     4201 ggtcggcggt ccggcggagc cgctcctcgg ccacgacgag cagggacttc gcgatctcgg
     4261 cggcctcggc ccagccgacg tcgtccgcac gctgacgctg ctccaggacg gccttcgccg
     4321 agctgatcac ctggtcgaca cgctccttgc gcagcgagtc accgaccgcg cccggcgcct
     4381 cgagcagggt tcggcgctgg atgaaggcga acaggtcgac gaggcccttg aggtcgcccg
     4441 cgtcggcgcg gtcctgcgca gcggcgtagc gctcgaggtc gtcgcggcgg acgatcagcg
     4501 ggaaccaccc gttcttgacg aagatcgcgg tggcgagcgc gcgggcgatc agcccgttgc
     4561 cggcctggaa cggcgcgatc tgcatgaacc cgtgatggag ccacgccgcc tggacatcgg
     4621 cgggaagatc gcgcgcctgg tgcttctcgt gcagctcgag gagacgctcc atctcccagg
     4681 cgacctgttc cggagggcag aactggtggc tgctgccgtc cgggcgagtg acgtcgttcg
     4741 ggcccagctt gtacgcgccc ttcagcatgg gcccgccgtc gccggcgagg tgcagcgtga
     4801 tgagcgcgtg gagctccttg atgtcggacg tggagatgcc gcgcgggtcc gccgccatct
     4861 gctcgatccg gcggagcgcg aagagctgat cggccaccat ccccgcgagg agctcgggct
     4921 ccttcacgcc ggacgcggcg aggatcgacg cgtcgacgcc ctcgcggatc agccgctccg
     4981 acacgggtcg cgggagcgcg tagagaccct cgacgatccc cgtctcgacc gcccattcgt
     5041 tgcggagctg ctgctcgaac tccccgagct ccgccgtgcc ccgaagcgcg ctctgctgcg
     5101 ccgaccacga gctcgcgagc tgcgcgaggt cggggttggt cagcgtgcgg acgtcctcgg
     5161 ggaggtcgtc gatgctcttc cagcggtgtg atacagcctc agtcacgggc cttctcccat
     5221 gccgttcgcg gtcacgtaca tgcgacaaca tcggcacgag ggggcgccgt cttgaggccc
     5281 ggtggcccgt tcggactagt cgatgccctg ggccttctgg acctttccgc tgaagtcgac
     5341 cgagaccgac ttccactcca tgaactcgtc cagcgactgc ggaccggcct cgcggtggcc
     5401 gttgccggtg ttcttcatgc caccgaacgg aagctggatc tcggcgccga tcgtcggagc
     5461 gttcacgtag acgatcccga actcgagctg ctgcacggcc ttgaacacgt ttgtgatgtc
     5521 gtgcgtgtag atcgaggccg agagcccgta ctggaccgag ttcgcgatgc ggatcgcctc
     5581 ctcgacatcg tcggtgcgga tgatcccggt caccggcccg aagatctcct cctgggcgat
     5641 ccgcatgtcc ggcgtcatct catcgaagac ggtcggggcg atgaagttcc cgttctcgta
     5701 gccgttcccg gtcagctcct cgccgccggt caggaccttc gcgccctcgg ccttgccgat
     5761 ctcgatgtat tcgagcacgc gctgcttctg cttcgcgttg atcaccgggg cgagctcgac
     5821 gtcgggctcg aggccgctgc cgaccttcat cccgccgacg cgctcgagca accgctcggt
     5881 gaactcctgc cgggcggcgt cgtggacgac gagccgcgag gtcgccgtgc agcgctggcc
     5941 gctcgtggcg aacgcgcccc acgccgccgc ctcgaccgca aggtccatct gggcgtcggg
     6001 catcacgatc agcacgttct tcgagccgag ctccagggaa cagcgcttca tgaggcgccc
     6061 gcactgctcg ttgatcatcc ggccgacctc gagcgagccg gtgaacgaga tgccgcggac
     6121 gtcgtcgtgc gcgacgatcg gcgcgccggc ctcgtcgccg tagccgtgga cgaggttgag
     6181 gacgcccggc ggaagccc
//
LOCUS       DATLGG010000060         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_36745, whole genome shotgun sequence.
ACCESSION   DATLGG010000060 DATLGG010000000
VERSION     DATLGG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36745"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VM638_01575"
     CDS             complement(<1..598)
                     /locus_tag="VM638_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVM11153.1"
                     /translation="MPRQQRIYVPGGIFHLTGLAVAEEPLFRTDLDRHDFLALVRGLS
                     RRIDWQIVTWCLMSTHYHLLVALRRQTHVSQILQRLNATYAAAHNARYGRRGHLFGSR
                     YGYTPVEDGEHLDATIPYILENPVRAGLVARVEDWRWSGLDLLHPRPRPLRVPPPPRR
                     QDSRRSSAPPRPSARAASSPLPSIETPVLRMDELRGLSS"
     gene            complement(857..>2565)
                     /locus_tag="VM638_01580"
     CDS             complement(857..>2565)
                     /locus_tag="VM638_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HVM11154.1"
                     /translation="IILFIDELHNLVGAGAAEGAIDAASILKPALARGELQTIGATTL
                     DEYRKYLERDAALERRFQQIRVDEPSTDDTVQILRGLRDRYEAHHRVKITDEALEAAA
                     VLADRYIQDRHLPDKAIDLIDEAASRMRIKTMTAPPRYRELEEEIERVRKDKEESIEN
                     QEFEKAASLRDKERKLTQKKKELEENWRAEESEEQPVVGEDEIADIVSMWTGIPVFKL
                     TEAESQKLIRMEEELHKRVIGQHQAVVAVSKAIRRARAGIKDPKRPTGSFIFLGPSGV
                     GKTELARTLAEFLFGDEDAMIQIDMSEYMEKHSVSRLVGSPPGYVGYDEGGQLTEAVR
                     RKPYSVLLLDEIEKAHPDVFNILLQILEEGKLTDSQGRKVDFRNTIVIMTSNIGAGQI
                     AKNQTLGFSLGDETGLTYDEMKGRVTGELKKVFRPELLNRIDEIIVFHKLAKEEILTI
                     VDLMLRRLREQMAGHEVAIELTDDAKELLVEQGFDPAMGARPLRRAIQRFIEDPLADF
                     VLGRELSPGATILVDRREGEIPEGELPVEIRIVEGDASAEKVTVPAAGEAAEPDESPT
                     DD"
BASE COUNT          347 a          880 c          902 g          436 t
ORIGIN      
        1 gcgaagagag gccgcgtagc tcgtccatac gaagaacggg ggtctcgatc gatggcagtg
       61 gagaagaagc cgcgcgcgcg gaaggccgcg gaggcgccga agaccgccgc gagtcctgcc
      121 gccggggcgg cggagggacg cgcagcgggc gcggccgggg gtggagcagg tcgaggcccg
      181 accagcgcca gtcctcgacg cgcgcgacga ggccggcgcg cacggggttc tcgaggatgt
      241 acgggatcgt cgcgtcgagg tgctccccgt cctccaccgg cgtgtagccg tagcgcgacc
      301 cgaagaggtg accgcgccgc ccgtagcgag cgttgtgcgc ggcggcgtag gtcgcgttca
      361 ggcgctggag gatctgcgac acgtgcgtct ggcggcgcag cgcgacgagc aggtggtagt
      421 gcgtgctcat caggcaccag gtgacgatct gccagtcgat gcggcgggag aggccacgga
      481 cgagggcgag gaagtcgtgc cggtcgaggt ccgtgcggaa gagcggctcc tcggcgacgg
      541 cgaggcccgt caggtggaag atcccgcccg ggacgtagat gcgctgctgg cgcggcatgc
      601 cgccgacggt accgacggcg cagctacgcg aagtccgatt tcacgtaccc gtcacgctct
      661 tgcgcatcta tttctccact cgccgcgtcc tctcgggaca ggctctggcg cccaaggtcg
      721 aagcagtgcc agaccccggt ctgacccgcg tccgcatcgg ggtctgaccc tgctctgacc
      781 ctgctctcgc acgccgaacc gccggcggcc gccggaacga gagaggccgc ctcgcggcgg
      841 cctctcggat cgggtgctag tcgtctgtcg gcgactcgtc gggctctgcc gcttcgccgg
      901 ccgccggcac ggtcaccttc tcggccgacg cgtcgccctc gacgatgcgg atctcgacgg
      961 gcagctcgcc ctccgggatc tcgccctcgc ggcggtcgac gaggatggtc gcgcccggcg
     1021 acagctcgcg cccgagcacg aagtccgcga gcgggtcctc gatgaaccgc tggatcgcgc
     1081 ggcgcagcgg ccgggcgccc atcgcggggt cgaagccctg ctcgacgagc agctccttcg
     1141 cgtcgtcggt gagctcgatc gcgacctcgt gccccgccat ctgctcgcgc agacgccgca
     1201 gcatcaggtc gacgatcgtg aggatctcct ccttcgccag cttgtggaag acgatgatct
     1261 cgtcgatccg gttcagcagc tccgggcgga agaccttctt cagctcgccc gtgacgcgcc
     1321 ccttcatctc gtcgtacgtg aggcccgtct cgtcgccgag ggagaacccg agcgtctggt
     1381 tcttcgcgat ctgaccggcg ccgatgttcg acgtcatgat cacgatcgtg ttgcggaagt
     1441 cgaccttccg cccctgcgag tcggtgagct tcccctcctc gaggatctgg agcaggatgt
     1501 tgaagacgtc cgggtgcgcc ttctcgatct cgtcgaggag gagcaccgag tacggcttgc
     1561 gccgcacggc ctccgtgagc tggccgccct cgtcgtaccc gacgtagccg ggcggggagc
     1621 cgacgagccg ggacacggag tgcttctcca tgtactccga catgtcgatc tggatcatcg
     1681 cgtcctcgtc gccgaagagg aactcggcga gcgtgcgcgc gagctccgtc ttcccgacgc
     1741 cggacgggcc gaggaagatg aacgagccgg tcggccgctt cgggtccttg atcccggcgc
     1801 gggcgcggcg gatcgccttc gagaccgcga ccaccgcctg gtgctgcccg atcacgcgct
     1861 tgtgcagctc ctcctccatg cggatcagct tctgcgactc ggcctcggtg agcttgaaga
     1921 ccgggatgcc cgtccacatc gagacgatgt cggcgatctc gtcctcgccg acgaccggct
     1981 gctcctcgga ctcctcggcg cgccagttct cctcgagctc cttcttcttc tgcgtgagct
     2041 tgcgctcctt gtcgcgcagc gacgcggcct tctcgaactc ctggttctcg atcgactctt
     2101 ccttgtcctt gcggacccgc tcgatctcct cctcgagctc gcggtagcgc ggcggggcgg
     2161 tcatcgtctt gatgcgcatc cgggaagcgg cctcgtcgat caggtcgatc gccttgtcgg
     2221 gcaggtggcg gtcctggatg tagcggtcgg cgagcaccgc cgccgcctcg agcgcctcgt
     2281 cggtgatctt gacccggtgg tgcgcctcgt agcggtcgcg cagcccgcgg aggatctgca
     2341 cggtgtcgtc ggtcgacggc tcgtcgacgc ggatctgctg gaagcgccgc tcgagcgccg
     2401 cgtcgcgctc gaggtacttc cggtactcgt cgagcgtcgt cgcgccgatc gtctgcagct
     2461 cgccgcgcgc gagcgcgggc ttcaggatcg acgcggcgtc gatcgcgccc tcggcggcgc
     2521 cggcgccgac gaggttgtgg agctcgtcga tgaacaggat gatgt
//
LOCUS       DATLGG010000061         2697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_161120, whole genome shotgun sequence.
ACCESSION   DATLGG010000061 DATLGG010000000
VERSION     DATLGG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161120"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="gltX"
                     /locus_tag="VM638_01585"
     CDS             complement(<1..881)
                     /gene="gltX"
                     /locus_tag="VM638_01585"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVM11155.1"
                     /translation="MADRQVRVRYAPAPSGDLHVGGARTALYDWLFARHHGGVFVLRI
                     EDTDQSRVTEESFLGLQDALRWLGIDWDEGPGVGGAFGPYRQTERLPIYREAVETLIE
                     RDAAYRCYCTPEELEQRRKEALARGEPPGYDGRCRRLSAGERAAFEAEGRPFATRFAT
                     PGRDVTVHDLVRGEATFRAPDIRDFVILRSDGSPTYLLAAAVDDWQMRMTHVIRGEDL
                     FSSTPRQLLLMEALGVEDPPAYGHLPLIVGPDRQPLSKRHGSVAVERFREEGFLPEAM
                     INYLALLGWSFDEKTTF"
     gene            complement(874..1635)
                     /locus_tag="VM638_01590"
     CDS             complement(874..1635)
                     /locus_tag="VM638_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVM11156.1"
                     /translation="MRLARLRLGDRIATAAIEGEVARLLAGTFFEDPVPTGEELPVDE
                     VRLLAPAIPSKIVCIGRNYREHAEELGNPLPDHPMLFLKPSTAVIGPGDPIPLPAVSR
                     RVDHESEMAAVIGRLCRKVSPDEALRYVLGYTCANDVTARDLQKPDGQWGRAKGFDGF
                     CPLGPWIETEVDVDDIEVSCRVNGEVRQSGRTSTMAFPPAQLISYISHVMTLLPGDVV
                     LTGTPGGVSPLEPGDVVEVEVEGVGVLENPVGPRG"
     gene            complement(1658..2581)
                     /locus_tag="VM638_01595"
     CDS             complement(1658..2581)
                     /locus_tag="VM638_01595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HVM11157.1"
                     /translation="MPIQPVDKVWMDGKLIDWEDARIHVVSHALHYGTGVFEGIRAYE
                     TVRGPAIFRLTDHMKRLLRSAHIYAMDVPFGLEELVEGAKETVRENRLRACYVRPIVW
                     RGYGEMGLNFLPASVNVAIAVWEWATYLGQDCLDNGARVMVSSWKKHDPNIIPPAAKA
                     TGQYINSGLAKMQAVKAGYDDAIMLSHDGYVADGTGENLFVVRGETLHTPPEGSGILL
                     GITRDSVMRIARDLGYEVIERELVRSDLYTADEVFFTGTAAEVTPIREVDDRTVGTGR
                     PGPVTKRIQETYFRAVKGELPAYEDWCEYAG"
BASE COUNT          403 a          939 c          960 g          395 t
ORIGIN      
        1 aagaaggtcg tcttctcgtc gaaggaccag ccgagcagcg ccaggtagtt gatcatcgcc
       61 tcggggagga agccctcctc gcggaagcgc tcgacggcca cggagccgtg ccgcttcgaa
      121 agcggctggc ggtcgggccc gacgatcagc ggcaggtgcc cgtaggccgg tgggtcctcg
      181 accccgagcg cctccatgag cagaagttgg cgcggtgtcg aggagaagag gtcctcgccc
      241 cggatcacgt gcgtcatgcg catctgccag tcgtcgacgg ccgccgcgag gaggtacgtc
      301 ggggagccat cgctccgcag gatcacgaaa tcgcggatgt ccggcgcccg gaaggtcgcc
      361 tcgccgcgga cgaggtcgtg gaccgtgacg tcgcgcccgg gtgtggcgaa gcgggtggcg
      421 aacggccgtc cctccgcctc gaacgcggcg cgctcccctg ccgagaggcg ccggcaccgg
      481 ccgtcgtagc ccggcggctc gccccgcgcg agcgcctcct tgcggcgctg ctcgagctcc
      541 tccggggtgc agtagcagcg ataggcggcg tcgcgctcga tgagcgtctc gaccgcctcg
      601 cggtagatcg gcaggcgctc ggtctgacgg tagggaccga acgcgccccc aaccccgggg
      661 ccctcgtccc agtcgatccc cagccaacgc agggcatcct ggagcccgag gaacgactcc
      721 tcggtgacgc ggctctggtc ggtgtcctcg atccgcagga cgaagacgcc gccgtggtgc
      781 cgcgcgaaca gccagtcgta gagcgccgtg cgggcgccgc cgacgtggag gtccccgctc
      841 ggcgccggtg cgtaccggac ccggacctga cggtcagcca cgcgggccaa ccgggttctc
      901 gagcacgccc acgccctcga cctcgacctc gacgacgtcc ccgggctcca gcggggaaac
      961 accccccggg gttccggtga ggacgacgtc gccgggcagg agggtcatga cgtgggagat
     1021 gtaggagatg agctgcgccg gcgggaaggc catcgtcgac gttcgccccg actgccgcac
     1081 ctcgccgttc acccggcacg agacctcgat gtcgtcgacg tccacctcgg tctcgatcca
     1141 cggcccgagc ggacagaagc cgtcgaagcc cttcgcccgt ccccactgcc cgtcgggctt
     1201 ctggaggtcc cgggccgtga cgtcgttcgc gcacgtgtag ccgaggacgt agcgcagggc
     1261 ctcgtccggg gagaccttcc ggcagagccg accgatcacc gcggccatct cgctctcgtg
     1321 atcgacgcgg cgagacaccg ccgggagcgg gatcggatcg ccgggaccga tgacggccgt
     1381 cgagggcttg aggaagagca tcggatggtc gggcaacggg ttgccgagct cctcggcgtg
     1441 ctcccggtag ttgcgtccga tgcagacgat cttcgacggg atcgccggcg ccagcaggcg
     1501 cacctcgtcg acgggaagct cctcgcccgt gggaacgggg tcctcgaaga aagtgccggc
     1561 gagcaggcgc gcgacctcgc cctcgatggc ggcggtggcg atgcggtcgc cgaggcggag
     1621 tcgagccagt ctcacgggcc gagcctatcc gagcggctca gccggcgtat tcgcaccagt
     1681 cctcgtacgc gggcagctcg cccttcacgg cccggaagta cgtctcctgg atgcgcttcg
     1741 tcaccggtcc ggggcggccc gtgccgaccg tgcggtcgtc gacctcgcgg atcggcgtca
     1801 cctcggcggc ggtgccggtg aagaagacct cgtccgcggt gtagaggtcc gagcggacga
     1861 gctcgcgctc gatcacctcg tagccgaggt cgcgcgcgat gcgcatgacg ctgtcgcgcg
     1921 tgatcccgag gaggatcccg ctgccctcgg gcggcgtgtg cagggtctcc ccccggacga
     1981 cgaagaggtt ctcgccggtg ccgtcggcca cgtagccgtc gtgcgagagc atgatcgcgt
     2041 cgtcgtaccc ggccttgacg gcctgcatct tcgcgagccc ggagttgatg tactgcccgg
     2101 tggccttcgc tgccggcggg atgatgttcg gatcgtgctt cttccacgac gagaccatca
     2161 cgcgcgcacc gttgtcgagg cagtcctggc cgaggtacgt cgcccactcc cacaccgcga
     2221 tcgcgacgtt cactgaggcc gggaggaagt tcagccccat ctcaccgtag ccgcgccaca
     2281 cgatgggacg gacgtagcag gcgcggagcc ggttctcgcg gacggtctcc ttcgctccct
     2341 cgacgagctc ctccaggccg aaggggacgt ccatcgcgta gatgtgggcg gagcgaagga
     2401 gccgcttcat gtggtcggtc agccggaaga tcgccggccc gcgcacggtc tcgtaggcgc
     2461 ggatgccctc gaagacgccg gtcccgtagt ggagcgcgtg ggagacgacg tggatgcggg
     2521 cgtcctccca gtcgatgagc ttcccatcca tccacacctt gtcgaccggc tggatcggca
     2581 tcgcgctggg cctcctcccg tcaggatccc cgcaagggta catcgccccc gaaggcgcga
     2641 gcggcctggg aacgaaggcg taacggaccc gttacacgag ggcgccgctc aggacgg
//
LOCUS       DATLGG010000062         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_83616, whole genome shotgun sequence.
ACCESSION   DATLGG010000062 DATLGG010000000
VERSION     DATLGG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83616"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1220)
                     /locus_tag="VM638_01600"
     CDS             complement(150..1220)
                     /locus_tag="VM638_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVM11158.1"
                     /translation="MPIVDYLRYMVEAGASDLHLKVGLPPVVRVDGVLMHSPFPPLTP
                     AETDALGQEIIPDTKRAEFAQRNEVDLGYTLEGVGRFRANAYRQRGDVSIALRRVRSE
                     IPTFQELRLPQVITQLAQEPRGLVLVTGPTGTGKTTTIASIIGWINANRRVHIVTIED
                     PIEVVHDDLQSVIQQREIGLDTESYSSALRHVVRQDPDVIFIGEIRDMETAEAAIQAA
                     ETGHLVISTLHTLDATETVNRIIDLYPPSMQRQARLSLASSLRGVLSQRLVPRADGKG
                     RVPAVEVLVNTGRVQERVIDADQTSSIPEVIADGDFYGMQTFDQALVKLVGEGLVTVE
                     AALESASNPHDLTLALRQAQLA"
     gene            complement(1207..1899)
                     /locus_tag="VM638_01605"
     CDS             complement(1207..1899)
                     /locus_tag="VM638_01605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVM11159.1"
                     /translation="MIERVVEFLRPYFADWGYVLVGTAGFLENSIGAGLILPGETIVI
                     LGGFYAASGDLSFVAVATIAAVAGALGDNVGYLIGNRFGRRVLDRFGRFLFLSPARVA
                     RAEDFYRRHGGKTVFLGRFIPVVRSLGCLIAGMSRLSYRRFVVYDLLGATIWAVGNTF
                     VGYLIGRGYERVERYLGGAGVAALLLLIVLVWLSAWWSRRRRRAEEGLNVGGPGADTS
                     GEPEEGHHDAHR"
     gene            2032..2205
                     /locus_tag="VM638_01610"
     CDS             2032..2205
                     /locus_tag="VM638_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018884717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11160.1"
                     /translation="MSRAEAGRRGGLSTKKRYGPGFYQEIGRKGGKKGGLTTKERYGV
                     EFYQQIGRKGGSR"
     gene            complement(2251..2673)
                     /gene="sodN"
                     /locus_tag="VM638_01615"
     CDS             complement(2251..2673)
                     /gene="sodN"
                     /locus_tag="VM638_01615"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HVM11161.1"
                     /translation="MSIVARIIRPRRVVHAHCDVPCGIYDPAQARIEAESVKAIQERY
                     QKAEEMKKPSESVEDYRTRCLLIKEHRADLVKHHLWVLWTDYFKPEHLEAYPELHDLF
                     WRATKAAGEAKKSQDPAQGQGLLDLIGEIDKIFWNTKK"
BASE COUNT          424 a          926 c          945 g          424 t
ORIGIN      
        1 cggacccacc gacgaggtag agctggtgcc cggctgcttc gaagcgggct ccgagctcgc
       61 cggcgatcgg gggcagggcg aagagatcgg aagaggaggt cgccacctgg cgaaggatac
      121 cgcaggccct gcggcccctc gccgagccct caggccagct gcgcctgccg gagcgcgagc
      181 gtgaggtcgt gcgggttcga cgccgactcg agcgcggcct cgaccgtcac gagtccctcg
      241 ccgacgagct tcacgagcgc ctggtcgaag gtctgcatcc cgtagaagtc gccgtccgcg
      301 atcacctccg ggatcgacga ggtctggtcc gcgtcgatga cccgctcctg gacgcgcccg
      361 gtgttgacca ggacctcgac cgccggaacg cggcccttgc cgtcggcgcg cgggacgagg
      421 cgctgcgaga ggacaccgcg caggctcgag gcgagcgaga ggcgcgcctg tcgctgcatc
      481 gacggcgggt acaggtcgat gatccggttc acggtctcgg tcgcgtcgag cgtgtgcagg
      541 gtcgagatca cgagatggcc ggtctcggcg gcctggatcg cggcttcggc cgtctccatg
      601 tcgcggatct caccgatgaa gatcacgtcg gggtcttggc ggacgacgtg gcgcagcgcc
      661 gaggagtacg actcggtgtc gaggccgatc tcacgctgct ggatgacgga ctggaggtcg
      721 tcgtggacga cctcgatcgg atcctcgatc gtgacgatgt gcacgcggcg gttcgcattg
      781 atccagccga tgatcgaggc gatcgtcgtc gtcttgccgg ttcccgtcgg gcccgtcacg
      841 aggacgagtc cgcgcggctc ctgcgcgagc tgggtgatca cctgcgggag gcgcagctcc
      901 tggaaggtcg ggatctccga gcggacgcgc cgcagcgcga tgctgacgtc cccccgctgg
      961 cggtaggcgt tggcgcggaa acgaccgacg ccctcgagcg tgtacccgag gtcgacctcg
     1021 ttccgctggg cgaactcggc acgcttcgta tccgggatga tctcctgtcc gagcgcgtcg
     1081 gtttccgccg gtgtcagcgg cgggaacggc gagtgcatga gcacgccgtc aacccgcacg
     1141 acgggcggca ggccgacctt gaggtggagg tcgctcgcgc cggcctccac catgtaccgc
     1201 aagtagtcaa cgatgggcat cgtggtgtcc ctcctcaggc tccccactgg tatcggcacc
     1261 cgggcccccg acgttgagcc cctcttccgc ccgccgtctc cgccggctcc accaggccga
     1321 gagccacacg aggacgatga gcaggagcag cgcggcgaca ccggcgcccc cgaggtagcg
     1381 ctcgacgcgc tcgtacccgc gcccgatcag atagccgacg aaggtgttgc ccacggccca
     1441 gatcgtcgcg ccgaggaggt cgtagacgac gaaccgtcga tacgagagcc ggctcatccc
     1501 cgcgatcagg catccgaggc tgcggaccac cgggatgaac cgcccgagga acacggtctt
     1561 gccgccgtgc cgccggtaga agtcctcggc acgcgcgacc cgcgcgggcg agaggaacag
     1621 gaaccggccg aagcggtcca ggacgcgccg cccgaagcgg ttgccgatca ggtagccgac
     1681 gttgtccccg agcgccccgg ccaccgcagc gatcgtcgcg acggccacga acgacaggtc
     1741 gccgctggcc gcgtagaagc ctccgaggat cacgatcgtc tccccgggaa ggatgagccc
     1801 ggcgccgatc gagttctcga ggaaccccgc cgtcccgacc aggacgtagc cccagtcggc
     1861 gaaatacggc cgtaggaact cgacgacgcg ctcgatcatg ccggaccccc gcgggagtct
     1921 agcccctcgc gcccggccag ctggtccgtc gcgtccgtct cgtttccctt gtccggaggt
     1981 ggggtaggaa cggcctgcac tgaggaggtg gtgtgcgaga tggcgcagcc gatgtcgaga
     2041 gccgaagctg gtcgtcgcgg cgggctcagc accaagaagc ggtacggccc cggcttctac
     2101 caggagatcg gccgcaaggg tggcaagaag ggcggcctga ccacgaaaga gcgctacggc
     2161 gtcgagttct accagcagat cggccgcaag ggcggatcgc gctgatcgta tcggggccgg
     2221 ctccggccgg ccccgatccg tacctcgctc ctacttcttc gtgttccaga agatcttgtc
     2281 gatctcgccg atgagatcga ggagcccctg gccctgggcc gggtcctgcg acttcttcgc
     2341 ttctccggcc gccttcgtgg cgcgccagaa caggtcgtgc agctcggggt acgcctcgag
     2401 gtgctcgggc ttgaagtagt cggtccacag gacccagagg tggtgcttca cgagatcggc
     2461 acggtgctcc ttgatcagga ggcaccgggt ccggtagtcc tccacgctct cggagggctt
     2521 cttcatctcc tcggccttct ggtaccgctc ctggatcgcc ttcacggact cggcttcgat
     2581 ccgcgcctgc gccggatcgt agatgccgca gggaacgtcg cagtgcgcgt ggaccacgcg
     2641 ccggggccgg atgatgcgcg cgacgatcga catcggtcct ccttttcgtc ggggtcgttc
     2701 cacgccctta ccctatccc
//
LOCUS       DATLGG010000063         6875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_36810, whole genome shotgun sequence.
ACCESSION   DATLGG010000063 DATLGG010000000
VERSION     DATLGG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36810"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VM638_01620"
     CDS             <1..274
                     /locus_tag="VM638_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11162.1"
                     /translation="RPVLELHGFGDLVTPLRDAYRAGDLVKLAEIAMPMVDTYAIAGD
                     EDACREGLARFDGLLDRVILGSAWVGPPEAVVENHRRLLHTFRPAG"
     gene            complement(271..2184)
                     /gene="ftsH"
                     /locus_tag="VM638_01625"
     CDS             complement(271..2184)
                     /gene="ftsH"
                     /locus_tag="VM638_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVM11163.1"
                     /translation="MADEKRGGRWQGFDLGGGSGGSGPRRWRLPLGYLLVALLGVWLL
                     QSLVAPGPTRVDYSAFLDRVERGQVERVAISEATITGEYVEEGRRVEFTTTRPPGVDE
                     RDLLQLLQREEVAFTGEQPGALSSFILSWILPILFLVLIWSFIFRRMAGGGAAAGALN
                     LGKRGVKIYDRKDLKTTFADVAGVDEAVEELGEIVEFLSNPKKYQRLGGRIPKGVLLL
                     GPPGCGKTLLARAVAGEAGVAFFYMSGSEFVEMFVGLGAARVRELFQQAKERAPCLVF
                     LDELDTIGKTRGGAMGAGFGSHDEREQTLNQLLVEMDGFDASKGVIIMAATNRPDVLD
                     PALLRPGRFDRQVVVDRPDLRGREAILKVHARGVSLADDIDLHVIAARTPGFTGADLA
                     NVVNEAALLAARREKDAVTMSELEEAIDRVVAGLERKSRVMSDREREIVAFHEMGHAL
                     VALSVENADPVHRVTIIPRGTAALGMTMQRPLEDRYLMTEPELADRLAILLGGRTAEE
                     IAFGQVSTGAQNDLERATEIARSMVAEYGMSEKLGPLSFGRDGFRHSDGRAMFPGQGA
                     DVSDQTARLIDEEVGRIVGEAHQRARTILADRRDLLERLAKLLMDREVLEGGELKELV
                     EGEVPHEERLPAD"
     gene            2247..2849
                     /locus_tag="VM638_01630"
     CDS             2247..2849
                     /locus_tag="VM638_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM11164.1"
                     /translation="MSDTGHATEPLHQPEAEDRFRREALPLVDELYGAALRLTRNPSD
                     AEDVVQETYLRAYRGFAGFRPGTNLRAWMHRILMNVFISGYRKRRREPQTVSEEEIPE
                     WSLYDRLVGAGAEPSAEAEALARLPDEDVQRALEEVPERFRAPVLLADVEGFSYKEIA
                     EILGVPIGTVMSRLHRGRKALEKALWEVMRHRGMIEGGEA"
     gene            2864..3091
                     /gene="rsrA"
                     /locus_tag="VM638_01635"
     CDS             2864..3091
                     /gene="rsrA"
                     /locus_tag="VM638_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HVM11165.1"
                     /translation="MSCAEVLAKLETYCDGELSRWDRRRLERHLAACPPCVDRREFRT
                     TLQQVVRDKCARSETPPDLLERIRRSLSSGP"
     gene            3217..3390
                     /locus_tag="VM638_01640"
     CDS             3217..3390
                     /locus_tag="VM638_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM11166.1"
                     /translation="MKTAEVADMLHVSPKTVTRWAKDGKLPHSRTLGGHRRFPAEAIR
                     RLAEQLVQVDNER"
     gene            complement(3398..3712)
                     /locus_tag="VM638_01645"
     CDS             complement(3398..3712)
                     /locus_tag="VM638_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11167.1"
                     /translation="MRIHRDVALAGTLVLAVVGGCAPDVADLPQPLEGKVIEVRREGA
                     DIAGFTVSGDDVEEEILIDREHDYGFDLNHLEEHRRTGDPVRVRWAQRGDRAYAVTIE
                     DA"
     gene            3834..4412
                     /locus_tag="VM638_01650"
     CDS             3834..4412
                     /locus_tag="VM638_01650"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HVM11168.1"
                     /translation="MEMVGQEKRRSSAKVGLTLMVVAVLGAVVGIGTWSAFSSTTENS
                     GNVFQAGTVTIGDNDGNSFLFDLSNLKPGDTSEKCIEVSYTGSLPASVKLYGTGNGSL
                     GQYLTLTVTKGSITGPTFPSCTGFTPADGGGQIYSGNLSSFPASLGAALDEGTWNEGQ
                     KRVYRLAISVQDNEAAQGQSRTQSFTWDARSS"
     gene            4412..4933
                     /locus_tag="VM638_01655"
     CDS             4412..4933
                     /locus_tag="VM638_01655"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVM11169.1"
                     /translation="MQAVGVPARSGGVRLWLDRAALAAVGVALLAVGAVASAVVLGYR
                     PLLVRSGSMAPAIHTGDVVLSRLVEAREISVGDVVTFRDPTRDQRLITHRVVGMRPQA
                     QAIAFVTRGDANTGEERWSVARDGTVGRVSMRVPKVGYVLAWIGLPIVRMSVLLLGCL
                     LVGWALIRRIWDG"
     gene            5009..5782
                     /locus_tag="VM638_01660"
     CDS             5009..5782
                     /locus_tag="VM638_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11170.1"
                     /translation="MLVAGLLVLGAGYASAAARIRVKAPAPGICTGETITLGVRKVGT
                     RPSYTVTVFRPNGTKMWSRSGTATTRWKTWNVTLPQPGTYTTKYVGYKKRWTFQTVVT
                     NCATLSMGNVDSAVLALENAAPGDAVEACVRVAYSGVPATVSLYGTTQGTGLDAYLEL
                     SVTRGSMPAGGEAGSCEGFVEDPTDYLGHGDGVMYRGSLQAFPDHPDAGLADPVATAP
                     AVWADGEAHAYRLRVELGADDRAQGRSASQRFIWGARPA"
     gene            5782..6771
                     /locus_tag="VM638_01665"
     CDS             5782..6771
                     /locus_tag="VM638_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11171.1"
                     /translation="MRARGTLLLVVWLAGAAVGTSVGPTFAAFSATTQNTGNTFAGAP
                     DWAGPSVPAATIRKGSGGLPGFIRPGGTYRVFALVTDQGNPPSGVQAVTANVGNVTSG
                     QTAVTMSSGEWSVGGVSYNYRSAELTANAVMTDGAKSFSIKGRDVLGNERTVGGFSVT
                     VDSVVPVGMDIQTENVHGGTLGRPEVGDTVLYTFSEQMEPDSIVSGWSGSATNVTVRF
                     INNGGGGASANDRVQIWNASNSTQLPLGQIDTQGNYVTANVTFGASGTPSTMVQSGSV
                     IKITLGTVSDPIAIATNGNDTQLWTPASAATDLAGNNCDVAVVTELGILDPDF"
BASE COUNT         1021 a         2417 c         2455 g          982 t
ORIGIN      
        1 ccgcccggtc ctcgagcttc acgggttcgg cgacctcgtg acgccgctcc gcgacgccta
       61 ccgcgccggc gacctcgtga aactcgccga gatcgcgatg cccatggtcg acacctacgc
      121 gatcgccggt gacgaagacg cctgccgcga gggcctcgcc cgcttcgacg gcctgctcga
      181 ccgtgtgatc ctcggcagcg cctgggtcgg cccccccgaa gcggtggtcg agaaccaccg
      241 ccgcctcctg cacacgttcc gccccgcggg ctagtcggcg ggcagtcgct cctcgtgagg
      301 aacctcgccc tcgacgagct ccttgagctc gccgccctcg aggacctcgc ggtccatcag
      361 gagcttcgcc aggcgctcga gcaggtcccg ccgatcggcg aggatcgtcc tggcgcgctg
      421 gtgcgcctcg ccgacgatcc ggccgacctc ctcgtcgatc aggcgagccg tctggtcgga
      481 gacgtcggcc ccctgacccg ggaacatcgc gcgtccgtcg ctgtggcgga agccgtcccg
      541 gccgaaggag agcggcccca gcttctcgct catcccgtac tcggcgacca tcgaccgcgc
      601 gatctcggtc gcgcgctcga ggtcgttctg ggcgccggtc gagacctggc cgaaggcgat
      661 ctcctcggcc gtccggccgc cgagcaggat cgcgaggcgg tcggcgagct ccggctccgt
      721 catgaggtag cggtcctcga gggggcgttg catcgtcatc ccgagggccg cggtcccgcg
      781 agggatgatc gtcacccggt gcaccgggtc ggcgttctcg acggaaagcg ccacgagcgc
      841 atgtcccatc tcgtggaacg cgacgatctc ccgctcgcgg tcgctcatca cgcgcgactt
      901 ccgctcgagc cccgcgacca cccggtcgat cgcctcctcg agctccgaca tggtcaccgc
      961 gtccttctcg cgccgggccg cgaggagcgc cgcctcgttc acgacgttcg cgaggtcggc
     1021 tcccgtgaac cctggggtac gggcggcgat cacgtggagg tcgatgtcgt cggcgagcga
     1081 gaccccgcgc gcgtgcacct tcaggatggc ctcccgcccg cggaggtcgg gccggtcgac
     1141 gacgacctgg cggtcgaagc ggcccggccg caggagcgcg gggtcgagga cgtccgggcg
     1201 gttcgtcgcg gccatgatga tcacgccttt gctcgcgtcg aagccgtcca tctcgacgag
     1261 gagctggttc agcgtctgct cccgctcgtc gtgggagccg aagccggcgc ccatcgcgcc
     1321 gccacgcgtc ttgccgatcg tgtcgagctc gtcgaggaag acgaggcacg gcgcacgctc
     1381 cttcgcctgc tggaagagct cgcgaacccg cgccgctccg aggccgacga acatctccac
     1441 gaactccgag ccggacatgt agaagaaggc cacgccggcc tcgcccgcga ccgcgcgcgc
     1501 gagcagggtc ttgccgcacc ccggcgggcc gagcaggagg acgcccttcg ggatccgccc
     1561 accgagccgc tggtacttct tcggattgct gaggaactcg acgatctcgc cgagctcctc
     1621 cacggcctcg tcgaccccgg cgacgtccgc gaaggtggtc ttcaggtcct tccggtcgta
     1681 gatcttcacg ccgcgcttgc cgaggttcag cgcgccggcc gcggccccgc cgcccgccat
     1741 ccgccggaag atgaagctcc agatcaggac caggaacagg atcggcagga tccacgacag
     1801 gatgaagctc gacagcgcgc cgggctgctc gcccgtgaac gcgacctctt ctcgctgcag
     1861 cagctggagg aggtcccgct cgtcgacgcc cgggggccgc gtcgtcgtga actcgacgcg
     1921 ccggccctcc tcgacgtact cacccgtgat cgtcgcctcc gagatcgcga cgcgctcgac
     1981 ctggccccgc tcgacgcggt cgaggaacgc ggagtagtcc acgcgcgttg ggcctggggc
     2041 gacgagcgac tggaggagcc acacgccgag gagggcgacc aggaggtacc cgagcggcag
     2101 acgccagcgg cgcggcccgc tccctccgga gcctccgccg aggtcgaacc cctgccagcg
     2161 cccgccgcgc ttctcgtcgg ccacgggtcc tatgttccca catgggtgcg ggaggccccg
     2221 gagaggggcg tgggtatgct tcgtcggtga gcgacaccgg ccacgcgacg gagcccctcc
     2281 accagcccga ggcggaggat cgcttccggc gggaggcgct cccgctggtc gacgagctct
     2341 acggcgccgc cctgcggctc acgcgcaacc cctcggatgc ggaggacgtc gtccaggaga
     2401 cgtatctgcg ggcctaccgc ggcttcgccg ggttccgacc ggggacgaac ctgcgggcct
     2461 ggatgcaccg gatcctgatg aacgtcttca tcagcggcta ccgcaagcgc cggcgcgagc
     2521 cccagaccgt gtccgaggag gagatcccgg agtggtccct gtacgaccgg ctggtgggag
     2581 cgggggccga gccgtccgcg gaggccgagg cgctcgcgcg gctcccggac gaggacgtcc
     2641 agcgggccct cgaggaggtc ccggagcgct tccgcgcccc ggtcctgctc gccgacgtcg
     2701 agggtttctc ctacaaggag atcgccgaga tcctcggcgt gcccatcgga accgtgatgt
     2761 cacggctcca ccgtggaagg aaagcgctgg aaaaggcgtt gtgggaggtc atgcgacatc
     2821 gtgggatgat cgagggcgga gaggcgtgat gcgactgggg tcgatgtcgt gcgccgaggt
     2881 cctggcgaag ctggagacct actgcgacgg ggagctctcc cgctgggacc gtcgccggct
     2941 ggagcgccac ctcgccgcct gccccccctg cgtcgaccgg cgcgagttcc gcacgaccct
     3001 tcagcaggtc gtgcgcgaca aatgcgccag gagcgagacg ccgcccgatc tcctcgaacg
     3061 gatccgtcgg tccctctcgt ccggcccgta ggagggtccc gcccccgcgt ccgatccgtc
     3121 ctatacttgc gccggcgcgg gaccagcgag ggggtcgccc atcgggggga ccgtggccgg
     3181 gaggagggag agttggcgca gcaccagatc ggctacatga agacggcgga ggtcgcggac
     3241 atgcttcacg tctccccgaa gaccgtcacc cgctgggcga aggacggcaa gctcccgcac
     3301 tcgcggaccc tcgggggcca tcggcgtttc ccggccgagg ccatccgcag gctcgcggag
     3361 cagctcgtcc aggtcgacaa cgagcgctga tcgcgtctca ggcgtcctcg atcgtcaccg
     3421 cgtaggcgcg gtcgccccgc tgcgcccacc gcacgcgcac ggggtcgccc gtccggcgat
     3481 gttcctccag atggttgagg tcgaacccgt agtcgtgctc ccggtcgatc aggatctcct
     3541 cctcgacgtc gtcgcctgag accgtgaagc cggcgatgtc cgccccctcc cggcggacct
     3601 cgatgacctt cccctccagg ggctgcggga ggtccgcgac gtccggcgcg cagccaccga
     3661 cgaccgcgag gaccagggtg cccgcgaggg ccacgtcccg gtggatgcgc atccgccaag
     3721 cgtagacgag cgcctcctcg gccgtggtcg gtcgctgggg gtcccccgtc tggactaaag
     3781 gcgcctcccc acccacccga tgccatgggg gaagcggccc acaggaggga gctatggaga
     3841 tggtcgggca ggagaagcgg cggtcgtcgg cgaaggtcgg tctcacgctc atggtcgtcg
     3901 ccgtcctcgg ggcggtcgtg gggatcggga cctggtccgc gttcagctcg acgaccgaga
     3961 acagcggcaa cgtcttccag gcggggaccg tcacgatcgg ggacaacgac ggcaactcgt
     4021 tcctcttcga cctgtcgaac ctcaagccgg gcgacacgtc cgagaagtgc atcgaggtct
     4081 cctacacggg aagcctcccg gcgagcgtca agctctacgg gacggggaac ggcagcctcg
     4141 ggcagtacct gacgctgacc gtgacgaagg gatccatcac gggtccgacg ttcccttcct
     4201 gcacgggctt cacccccgcc gatggcggcg gtcagatcta ctccgggaac ctgtcgagct
     4261 tccccgcctc actcggcgcg gcgctcgacg aggggacctg gaacgaggga cagaagcgcg
     4321 tctaccggct ggcgatcagc gtccaggaca acgaggccgc gcagggccag agccgtacgc
     4381 agagcttcac ctgggacgcc cgcagcagct gatgcaggct gtcggggttc cagcccgctc
     4441 gggcggggtg cgcctgtggc tcgaccgggc cgcgctcgcc gcggtcggcg tggcgctcct
     4501 ggccgtcggt gcggttgcct cggccgtggt cctcgggtat cgcccgctcc tcgtccggag
     4561 cgggagcatg gcgccggcga tccacacggg cgatgtggtg ctttcccgcc tcgtggaggc
     4621 gcgcgagatc tccgtcggag acgtcgtgac cttccgcgat ccgacgcggg atcagcggtt
     4681 gatcacgcac cgcgtcgtgg ggatgcgacc gcaggctcag gcgatcgcat tcgtcacccg
     4741 cggcgacgcc aacacggggg aggagcgctg gagcgtcgcg cgcgacggga cggtcgggcg
     4801 cgtttcgatg cgggtcccca aggtcgggta cgtcctcgcg tggatcgggc tgccgatcgt
     4861 gcggatgagc gtcctgctgc tgggatgcct cctggtcggc tgggcgctga tccgccggat
     4921 ctgggacgga tgagggaccg ggcggatgtg ggcgcggacg accgctgagg ggcgccggca
     4981 gagccgcagg ccccggtcgg tcacggcggt gctcgtcgcc ggcctcctcg tgctgggggc
     5041 gggctacgcc tcggcggccg cgaggatccg cgtgaaggcg ccggctcccg ggatctgcac
     5101 cggcgagacg atcacgctcg gcgtgcggaa agtgggaacc cggccgagct acaccgtgac
     5161 cgtgttccgt ccgaacggca cgaagatgtg gagccggtcc ggcaccgcga ccacgcgatg
     5221 gaagacctgg aacgtcacgc tgccacagcc cgggacctac acgacgaagt acgtcggcta
     5281 caagaagcgc tggacgttcc agaccgtcgt gacgaactgc gcgacgctct cgatggggaa
     5341 cgtcgattcg gcggtcctgg cgctggagaa cgccgcaccg ggcgacgcgg ttgaggcatg
     5401 tgtgcgggtc gcgtactcgg gcgtccccgc gaccgtcagc ctctacggga cgacccaggg
     5461 gacggggctc gacgcctacc tcgagctcag cgtgacccgc gggtcgatgc cggccggcgg
     5521 cgaggcgggc tcgtgtgagg ggttcgtcga ggacccgacg gactacctcg gacacggaga
     5581 cggcgtgatg taccgaggct cccttcaggc cttccccgac catccggatg cgggcctggc
     5641 ggatcccgtc gcaacggctc cggcggtgtg ggcggacggc gaggcgcacg cgtaccgtct
     5701 ccgggtcgag ctcggcgccg acgatcgcgc gcaggggcgt tcggcctccc agcggttcat
     5761 ctggggggcg aggcccgcgt gatgcgtgct cgaggcaccc tgctgctcgt cgtctggttg
     5821 gcgggcgccg ccgtcggcac gagcgtcggg cccaccttcg ccgcgttctc cgcgaccacc
     5881 cagaacacgg ggaacacctt cgcgggtgcc ccggactggg cgggcccgag cgtcccggcg
     5941 gccacgatcc gcaaagggtc cggcggcctg cccgggttca tccgccccgg tggcacgtat
     6001 cgggtcttcg cgctcgtgac ggatcagggg aacccgccga gcggggtcca ggccgtgacg
     6061 gccaacgtcg gcaacgtgac gagtggccag acggccgtga cgatgagctc cggcgaatgg
     6121 tccgtcggcg gcgtcagcta caactaccgg agcgccgagc tgacggcgaa cgccgtcatg
     6181 acggacgggg cgaagtcatt ctcgatcaag ggtcgcgacg tgctcgggaa cgaacggacc
     6241 gtgggcgggt tctcggtcac ggtggacagc gtcgttcccg tcgggatgga catccagacc
     6301 gagaacgtgc acggcgggac ccttgggcgc cccgaggtcg gcgacaccgt cctgtacacg
     6361 ttcagcgagc agatggagcc ggactcgatc gtgtcggggt ggagcggctc ggcgaccaac
     6421 gtgacggtcc gcttcatcaa caacggcggc ggcggggcga gcgcgaacga ccgcgtccag
     6481 atctggaacg cctcgaactc gacgcagctg cccttgggac agatcgacac gcaggggaac
     6541 tacgtcacgg cgaacgtcac gttcggggcg agcgggacgc catccacgat ggtccagagc
     6601 ggctcggtca tcaagatcac gctcggaacc gtctccgacc cgatcgcgat cgccacgaac
     6661 ggcaacgaca cccagctgtg gacgccggcg agcgcagcga ccgacctcgc ggggaacaac
     6721 tgcgacgtcg cggtcgtgac cgagctcggc atcctcgacc cggacttctg agccaccgct
     6781 agactgcggc acgtgccgca catccgaacc gagcccggga cgatctggcg ggtcgtcctc
     6841 gcgcgtccgg agcagaggaa cgcgctctcg gagga
//
LOCUS       DATLGG010000064         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_208448, whole genome shotgun sequence.
ACCESSION   DATLGG010000064 DATLGG010000000
VERSION     DATLGG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208448"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..613
                     /locus_tag="VM638_01670"
     CDS             125..613
                     /locus_tag="VM638_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVM11172.1"
                     /translation="MDDSLAWRCPSCETPNPIEAEVCDRCGTPFRALFEEDERPAARV
                     DPGTAFARSLLFPGLGHRAAGRGAEGLARGVVFAWLIGTVAAILIVRGDAGPGPFLPL
                     IGALLLGALVVYAMTAVDARRAAERQKPLLTGRMLLYGSAGVMLLTIVVLFLSAVRAA
                     RG"
     gene            617..1381
                     /locus_tag="VM638_01675"
     CDS             617..1381
                     /locus_tag="VM638_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVM11173.1"
                     /translation="MRIKVISDLHGAVEALRREARDCDAMLVLGDLINVIDYHTMEGL
                     LVEVFGREPVAEAVGLRASGRYEEARQAMRRGVGDDLEARSRYVELATAEYERVFEAF
                     PEPTYVTYGNVDLPEVLRSFARPGVEIVDGGAFELGGERFGMVGGGLITPMNVPGEVP
                     EDEYDAKLEGLGPVDVVCTHIPPRIPWYVYDVLGHRFEPGSEGLIRYIRRHGPRRAYF
                     GHVHQPLTPRGHLWETELINVGHFRGSGRAFIHEGG"
     gene            1385..1831
                     /locus_tag="VM638_01680"
     CDS             1385..1831
                     /locus_tag="VM638_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVM11174.1"
                     /translation="MARTEGSIRIEAPPEEVMAVITDFETYPSWAGMRTAEVLETDEE
                     GRPQQVAFELEASGMQATYTLALDYPADHLGLAWVSTEAGGVVRHVEGAYRLEASGDG
                     TEVTYDLTVEPAIPLPGFLRRRAERAIVSTALEGLKRRVEEGGSGA"
     gene            1848..3044
                     /locus_tag="VM638_01685"
     CDS             1848..3044
                     /locus_tag="VM638_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HVM11175.1"
                     /translation="MRILLFTGKGGVGKTTIAAATAVRAARAGKRVLVTSTDPAHSLA
                     DSFDTPLGDVAAEVAPNLWAEQIDAQSRLESNWRDIQEYIIAFLNWTGIDAIEAEELS
                     VIPGLDEIFSLTDVKRHAESGSYDVLVIDCAPTAETLRLLSLPEIMNWYIERIFPVER
                     RIVNTVRPILTRITSMPIADDKIFAAVERLHRNLEGVRTILADAKRSSVRLVVNPEKM
                     VIAEARRTYTYLSLFGYRVDAVVANRIIPETVTDPYFGKWKDIQREHLGTIRDSFEPV
                     PILTARLFDQEMVGLDALERMGEEVYGEREATAVLHRDEPIRVRKRGGWYVLSMRLPF
                     ADRGDLDIHRKGDDLYIRVGSYKRNFILPQALQRLEVREARFIEDRLEVRFGEREATS
                     AGTAGG"
     gene            3064..>3602
                     /locus_tag="VM638_01690"
     CDS             3064..>3602
                     /locus_tag="VM638_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVM11176.1"
                     /translation="MSRTAIGIDVGGTKVAGALVDEAGSICARVVEETPADDVERTME
                     TIYRVGSNLARSGEPTAVGVGAAGMVDSAAGILRWAPNLAWKEIPIASLVADRLGLPC
                     LVDNDANAAAWGELRAGAARGRNDVLVVTVGTGIGGGIIAGGQLLRGAHGFAAEIGHI
                     IVEPGGPECGCGNRGCWEQV"
BASE COUNT          520 a         1242 c         1338 g          502 t
ORIGIN      
        1 gaacccggcc aacgtgacgg caggctcggc tcagcggccc gcccctccag ctgcgacacc
       61 tcgggatgag caggcgacat caccgggacg ccggaggggg gcgtccggcg gccccttcga
      121 ggtcgtggac gactcgctcg cgtggcggtg tccgtcctgc gagacgccga acccgatcga
      181 ggcggaggtg tgcgatcgct gcggcacgcc gttccgcgcg ctcttcgagg aggacgagcg
      241 gccggcggcg cgcgtcgatc cgggaaccgc cttcgctcgc tcgctcctgt tccccgggct
      301 cggccaccgc gcggcggggc gcggggcgga aggcctcgcc cgcggcgtcg tcttcgcctg
      361 gctgatcggg acggtcgccg cgatcctcat cgtgcgcggg gacgcggggc ccggaccctt
      421 cctcccgctg atcggagcgc tgctgctcgg cgccctcgtc gtctacgcga tgaccgccgt
      481 ggacgcccgg cgcgcggcgg agcggcagaa gccgctgctg acgggccgga tgctcctgta
      541 cggcagcgcc ggggtcatgc tgctgacgat cgtggtgctg ttcctgtcgg cggtccgcgc
      601 cgcccgcggt taggcgatgc gcatcaaggt catctccgac ctccacggcg ccgtcgaggc
      661 gctgcgccgc gaggcgcggg actgcgacgc gatgctcgtg ctcggtgacc tgatcaacgt
      721 gatcgactac cacacgatgg aagggctgct cgtcgaggtc ttcggacggg agccggtggc
      781 ggaggcggtg ggtctgcgcg ccagcggccg ctacgaggag gcccggcagg cgatgcggcg
      841 cggcgtcggc gacgacctgg aggcgcggag ccgctacgtg gagctcgcga ccgcggagta
      901 cgagcgggtg ttcgaggcct tccccgagcc cacctacgtg acgtacggca acgtcgacct
      961 ccctgaggtc ctgcgctcgt tcgcgcgtcc gggggtcgag atcgtcgacg gcggcgcctt
     1021 cgagctcggt ggggagcgct tcgggatggt gggcggaggc ctcatcacgc ccatgaacgt
     1081 gcccggcgaa gtgcccgagg acgagtacga cgcgaagctc gagggactcg gccccgtgga
     1141 cgtcgtctgc acccacatcc ctccgcggat cccctggtac gtctacgacg tcttgggcca
     1201 ccggttcgag ccgggctccg agggcctgat ccgctacatc cgacgccacg gcccgcgccg
     1261 ggcgtacttc ggccacgtcc accagccgtt gacgccgcgg ggccacctgt gggagaccga
     1321 gctcatcaac gtgggccact tccggggcag cggccgcgcg ttcatccacg agggaggctg
     1381 accgatggcg aggaccgagg ggagcatccg catcgaggcg ccgcccgagg aggtcatggc
     1441 ggtgatcacc gacttcgaga cgtacccgtc gtgggcgggg atgcgcaccg ccgaggtcct
     1501 cgagacggac gaggagggac gcccccagca ggtcgcgttc gagctcgagg cctccgggat
     1561 gcaggcgacc tacaccctcg cgctggacta cccggccgac cacctcggcc tcgcgtgggt
     1621 gagcaccgaa gcgggtgggg tcgtccgaca cgtcgagggc gcctatcggc tcgaggcgag
     1681 cggcgacggc accgaggtca cctacgacct cacggtcgag cccgcgatcc cccttccggg
     1741 gttcctccgg cgccgggcgg agcgggcgat cgtctcgacg gcgctcgagg ggctgaagcg
     1801 gcgcgtcgag gaggggggat ccggcgcctg acggtacgat gggcgccgtg aggatcctcc
     1861 tgttcaccgg caagggcggc gtcggcaaga cgacgatcgc tgcggcgacg gccgtccgcg
     1921 ccgcgcgggc gggcaagcgc gtgctcgtga cgagcaccga cccggcccat tcgctcgccg
     1981 actccttcga cacgccgctc ggcgatgtgg ccgccgaggt cgcgccgaac ctctgggcgg
     2041 agcagatcga cgcgcagtcg cggctcgagt cgaactggcg cgacatccag gagtacatca
     2101 tcgcgttcct caactggacg gggatcgacg cgatcgaggc cgaggagctc tcggtgatcc
     2161 cgggcctcga cgagatcttc agcctcacgg acgtgaagcg gcatgcggag agcgggagct
     2221 acgacgtcct cgtgatcgac tgcgccccca cggcggagac cctgcgcctg ctgtcgctcc
     2281 ccgagatcat gaactggtac atcgagcgga tcttcccggt cgagcgccgg atcgtgaaca
     2341 ccgtgcggcc gatcctcacg cgcatcacct cgatgccgat cgccgacgac aagatcttcg
     2401 cggccgtaga gcgtttgcac cgaaacctcg agggcgtgcg gacgatcctc gccgacgcca
     2461 agcgctcctc ggtccggctc gtggtcaacc ccgagaagat ggtcatcgcc gaggcgcgcc
     2521 gcacctacac gtacctctcc ctgttcggct accgcgtcga cgcggtcgtc gccaaccgca
     2581 tcatccccga gaccgtgacc gacccgtact tcggcaagtg gaaggacatc cagcgggagc
     2641 acctcgggac gatccgcgac tcgttcgagc cggtgccgat cctgacggct cggctcttcg
     2701 accaggagat ggtcggcctc gacgcgctcg agcggatggg ggaggaggtc tacggggagc
     2761 gggaggcgac cgccgtcctg caccgcgacg agccgatccg cgtccgcaag cgcggcggct
     2821 ggtacgtgct ctcgatgcgc ctgcccttcg ccgaccgcgg cgacctggac atccaccgca
     2881 agggcgacga cctctacatc cgggtcggct cctacaagcg caacttcatc ttgccgcagg
     2941 cgctgcagcg cctcgaggtt cgggaggccc gcttcatcga ggaccgcctc gaggtccggt
     3001 tcggggagcg cgaggccacg agcgccggaa ccgcgggcgg ctgacgggcg gggacgggac
     3061 cggatgagcc ggaccgcgat cgggatcgat gtcggcggaa cgaaggtcgc cggcgcgctg
     3121 gtcgacgaag ccgggtcgat ctgcgcccgc gtcgtcgagg agacgccggc cgatgacgtc
     3181 gagcgcacga tggagacgat ctaccgcgtc gggtcgaacc tcgcgcgctc cggtgagccg
     3241 acggcggtcg gcgtcggcgc ggcgggcatg gtcgactcgg ccgcggggat cctgcgctgg
     3301 gctccgaacc tcgcctggaa ggagatcccg atcgcgagcc tcgtcgcgga ccggctcggc
     3361 ctgccgtgcc tggtggacaa cgacgcgaac gccgccgcgt ggggggagct ccgcgccgga
     3421 gccgcgcgcg ggcggaacga cgtgctcgtc gtcaccgtcg gcacggggat cggcggcggg
     3481 atcatcgcgg gtgggcagct cctgcggggc gcgcacgggt tcgctgccga gatcggacac
     3541 atcatcgtcg agccgggcgg tcccgagtgc ggatgcggga accgaggatg ctgggagcag
     3601 gt
//
LOCUS       DATLGG010000065         4869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_192300, whole genome shotgun sequence.
ACCESSION   DATLGG010000065 DATLGG010000000
VERSION     DATLGG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192300"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VM638_01695"
     CDS             <1..462
                     /locus_tag="VM638_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11177.1"
                     /translation="LDGTTRITPSSRDAWRFEVFRCAPSCESAAVLAGGYGTTGDEVR
                     IAIPATVLGLRPDSGLTALRAFTGPGDALSGTLTRFDEVALPDAVIPEALVRFRVLHH
                     DEVVAEATSAPAAGRATALLQGPLAASSYEVEASLCLGSDCTPPRRVRTEV"
     gene            complement(466..1143)
                     /gene="hemQ"
                     /locus_tag="VM638_01700"
     CDS             complement(466..1143)
                     /gene="hemQ"
                     /locus_tag="VM638_01700"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HVM11178.1"
                     /translation="MAREEVIYASYPVYRRIRGRTPLTGHELSEATAELEGLFKEAAD
                     RVTIRGTYVAEGFRPDADLVCWWVGPSPDDLQDLAIRFRRTLLGRRLEPTWTFTGVHR
                     PAEFSPDHRPAFVRGEDPKRYLCVYPFVRTPEWYLLPKEERGRMLREHGEMGREFPEV
                     LANTTSAFGLGDWEWILAFEADEMHRIVDCIRRLRDAEARRFTKEEVPFVVGIRKSPA
                     EVVRDLA"
     gene            complement(1144..2067)
                     /gene="hemH"
                     /locus_tag="VM638_01705"
     CDS             complement(1144..2067)
                     /gene="hemH"
                     /locus_tag="VM638_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HVM11179.1"
                     /translation="MPEPLGILVMAYGTAAGPEDIERYYTDIRGGRPPSPEALHELKE
                     RYAAIGNTFPLDRITREQASALEREVATERPVRAYVGMKHSPPFVGDVVEQMRDDGIR
                     EAVGLVMAPHYSRMSIGGYVERVQRASADRGPRFTFVEQWHDHPAFVDVLSERVRAAR
                     GSLGQRQLENDLVVFTAHSLPARIVDEGDPYPAQLQETADLVAERLRLPRYRIGWQSE
                     GRTPEPWLGPPLDEIVRKAAADGHTALVVCPCGFVADHLEVLYDIDLEAQGVAREVGI
                     PLVRTESMNAEPEFVRALAHVVRDHLSERGG"
     gene            complement(2060..3100)
                     /gene="hemE"
                     /locus_tag="VM638_01710"
     CDS             complement(2060..3100)
                     /gene="hemE"
                     /locus_tag="VM638_01710"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HVM11180.1"
                     /translation="MSTAPFNDRFLRACRGEPVDATPVWFMRQAGRSLPEYRDLRGDT
                     DVLTLTRTPELAAEVCLQPVRRYDVDAAILFCDIMVPVAGVGIDVRIVKNQGPTLADP
                     LRTSEDLERLRPIEPESDVPFVFETIRLLRKELTIPLIGFAGAPFTVASYLVEGKPSR
                     EHIRTKSLMHAEPQVWAELMDRLTELTITYLRAQVDAGVQALQLFDSWVGALDLDDYR
                     RHVLPWSRRIHEGIAGLGVPTIHFGVGAAHLLEAIAETGADVVGLDWRVPLDEGWSRI
                     GPDRGVQGNLDPAACLGPWEVVERKARDVLRRAARRPGHIFNLGHGVLPETDPDILAR
                     LADLVHEGTVDA"
     gene            complement(3144..3914)
                     /locus_tag="VM638_01715"
     CDS             complement(3144..3914)
                     /locus_tag="VM638_01715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVM11181.1"
                     /translation="MGRSRTRERQLKRLAERREEERRRRRRKRVLAGVVAGLIALGGL
                     GVGAWAFLDARRDRAMGTSEGAVDPNEPVACGGAAPAAATQEKPTFAEPPEMQIDRDA
                     GYRAIMTTSCGTIELELFAKEAPKTVNSFVFLARQGFYDGLIFHRVIPGFMNQGGDPQ
                     GTGTGGPGYSFEDEFDPSLRFDEAGLLAMANSGANTNGSQFFITVGPTEHLNDRHTIF
                     GKVVEGMDVVERINTLPTTPEDRPTETVYIETVVIEET"
     gene            complement(3928..4869)
                     /locus_tag="VM638_01720"
     CDS             complement(3928..4869)
                     /locus_tag="VM638_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016645658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HVM11182.1"
                     /translation="MHRGERLAAVDMGTNSIRLLVAEVRQREEEPVELARDMVITRLG
                     RGVDRTGRLDPASLARTLEVLGWYAWRAKALGVRRIKVGATSAVRDAAEREELFSGVR
                     ALTGAEPEVLTGEREAALSFLGATRGLDAPTPYLVLDIGGGSTEAVVGTGSAERAISV
                     DVGSVRITERVAPVDPPHATEVEAMRAAAAAGLAPAFARIPAGSAATLVAVAGTATTV
                     QAIALGLERYDPDAIHRTTLHRRTVEETARRLAAMTTAERAALPVMVPGREDVIVAGV
                     QILLEAMERWGFERCLVSERDILDGLLLEMAALASAE"
BASE COUNT          691 a         1818 c         1643 g          717 t
ORIGIN      
        1 ctcgacggca cgacgaggat caccccttcc tcccgggacg cctggcgctt cgaggtgttc
       61 cggtgcgctc ccagctgcga gagcgcagcc gtcctcgccg ggggatacgg caccaccggc
      121 gacgaggtgc gcatcgcgat ccccgccacc gtcctcggcc tccggcccga cagcgggctc
      181 accgcactcc gggccttcac cggccccgga gatgccctca gcgggacgct gacccgcttc
      241 gatgaggtgg ccctccccga cgcggtcatc cccgaggccc tcgtgcggtt ccgcgtgcta
      301 caccacgacg aggtggtcgc cgaggccacc agcgctcccg cagccggacg agcgacggcc
      361 ctgctgcaag ggccgctcgc cgccagtagc tacgaggtcg aggcgagcct gtgcctcggc
      421 tcggactgca cgccaccgcg gcgcgttcga acggaggtct agcgctcagg cgaggtcgcg
      481 gacgacctcc gcgggcgact tgcggatgcc cacgacgaag gggacctcct ccttcgtgaa
      541 gcggcgggcc tcggcgtcgc ggagacgtcg gatgcaatcg acgatgcgat gcatctcgtc
      601 ggcctcgaag gcgaggatcc actcccagtc gccgagcccg aacgcgctcg tggtgttcgc
      661 cagcacctcg gggaactccc gtcccatctc gccgtgctcg cggagcatgc gaccgcgctc
      721 ctccttcgga aggaggtacc actcgggcgt gcgcacgaac ggatagacgc acaggtacct
      781 cttcggatcc tcaccgcgaa cgaaggcagg ccgatggtcg gggctgaact cagccgggcg
      841 atggacaccg gtgaaggtcc aggtcggctc gagccggcgg cccaggagcg tccttcggaa
      901 tcggatcgcc aggtcttgca ggtcgtcggg cgacggcccg acccaccagc acacgaggtc
      961 cgcgtcgggg cggaacccct cggcgacgta ggtcccgcgg atcgtcacgc gatcggcggc
     1021 ttccttgaac agcccctcga gctcagccgt cgcctcggag agctcgtggc ccgtcagcgg
     1081 cgtccgcccg cggatgcgcc ggtagaccgg gtacgacgcg tagatgacct cttcgcgtgc
     1141 cactcagccc ccccgctccg agaggtggtc gcgcacgacg tgcgcgagcg cgcggacgaa
     1201 ctcgggctcc gcgttcatcg actcggtccg caccagcggg atgccgacct cccgcgcgac
     1261 gccctgcgcc tcgaggtcga tgtcgtaaag gacctccaag tggtcggcga cgaacccaca
     1321 ggggcagacg acgagcgcgg tgtgaccgtc ggcggctgcc ttgcgcacga tctcgtccag
     1381 cggcggcccg agccacggct cgggcgtccg gccttcgctc tgccagccga tccggtagcg
     1441 cgggagacgc aggcgctccg cgaccaggtc cgcggtctcc tggagctgcg ccgggtacgg
     1501 gtctccctca tccacgatcc gcgccggaag gctgtgcgcc gtgaagacga cgaggtcgtt
     1561 ctcgagctgg cgttgaccga gcgaaccgcg cgccgcccgc acgcgctcgc tgagcacgtc
     1621 gacgaacgct gggtggtcgt gccactgctc cacgaacgtg aaccgcggac cccggtctgc
     1681 cgaggcgcgc tgcacgcgct cgacgtagcc accgatcgac atccgcgagt agtgcggcgc
     1741 catcacgaga ccgaccgcct cgcggatccc gtcgtcgcgc atctgctcca cgacatcccc
     1801 cacgaatggc ggggagtgct tcatcccgac gtacgcgcgc accggacgct ccgtcgccac
     1861 ctcgcgctcg agcgcggacg cctgctcccg cgtgatgcgg tcgagcggga aggtgttccc
     1921 gatcgcggcg taccgctcct tcagctcgtg cagcgcctcc gggctcgggg gccggccgcc
     1981 ccggatgtcc gtgtagtagc gctcgatgtc ctcgggtccg gcggcggtcc cgtacgccat
     2041 cacgaggatc ccgagcggct caggcatcga ccgttccctc gtgcacgagg tcggcgagac
     2101 gcgcgaggat gtcgggatcg gtctccggca ggacgccgtg accgaggttg aagatgtgac
     2161 cgggccgtcg cgccgcgcgc cgcagcacat cccgcgcctt ccgctcgacg acctcccacg
     2221 ggccgaggca cgccgcggga tcgaggttcc cctgcacccc tcggtccggg ccgatccggc
     2281 tccagccctc gtcgagcggc acgcgccagt cgagcccgac gacgtcggct cccgtctccg
     2341 cgatcgcctc cagcaggtgg gccgccccga cgccgaaatg gatcgtcggc acgcccagcc
     2401 cggcgatgcc ctcatggatc cggcggctcc acggcaggac gtgtcgccgg tagtcgtcga
     2461 ggtcgagagc cccgacccag gagtcgaaga gctggagcgc ctgtacgccc gcgtcgacct
     2521 gcgcacggag ataggtgatc gtgagctcgg tgaggcggtc catcagctcc gcccatacct
     2581 gcggctcggc gtgcatcagg ctcttcgtcc ggatgtgctc acgcgagggt ttcccctcga
     2641 ccaggtacga cgcgaccgtg aacggcgcgc ccgcgaaccc gatcaacggg atcgtgagct
     2701 ccttccggag gaggcggatc gtctcgaaca cgaacgggac gtcgctctcg ggctcgatcg
     2761 ggcggagccg ttcgaggtcc tcgctcgtac gcagcgggtc ggcgagcgtc ggaccctggt
     2821 tcttcacgat ccggacgtcg atccccacgc ccgcgaccgg gaccatgatg tcgcagaaga
     2881 ggatcgccgc gtcgacgtcg tagcgccgga ccggctggag gcatacttcg gcagcgagct
     2941 cgggcgtccg cgtcagggtc agcacgtcgg tgtcgccgcg cagatcgcgg tactccggaa
     3001 gcgaccgccc cgcctgtcgc atgaaccaga ccggcgtcgc atccacgggc tcgccgcggc
     3061 aggcgcgaag gaaccgatcg ttgaaggggg ccgtgctcac gccgtcagtc tagccgctga
     3121 gggcgaagcg acggctggca gccctaggtc tcctcgatca cgaccgtctc gatgtagacg
     3181 gtctccgtcg ggcggtcctc cggcgtggtc ggcagcgtgt tgatccgctc gacgacgtcc
     3241 atcccctcga cgacctttcc gaagatcgtg tggcggtcgt tgaggtgctc cgtcgggccg
     3301 acggtgatga agaactgact gccgttggtg ttcgctcccg agttcgccat cgcgagcaga
     3361 cccgcttcgt cgaaccggag gctcgggtcg aactcgtcct cgaacgaata cccgggcccg
     3421 cccgtgccgg tcccttgcgg gtccccgccc tggttcatga acccagggat cacccggtgg
     3481 aagatcaggc cgtcgtagaa gccctgcctc gcgaggaaca cgaagctgtt gacggtcttc
     3541 ggggcctcct tcgcgaagag ctcgagctcg atcgtcccgc aggaggtcgt catgatcgct
     3601 cgatatcccg cgtcgcgatc gatctgcatc tccggcggct cggcgaacgt cggcttctcc
     3661 tgcgtcgccg ccgccggggc tgctcccccg caggcgaccg gctcgttcgg gtcgaccgct
     3721 ccttcgctcg tccccatggc tcgatccctc ctcgcgtcga ggaacgccca cgcacccact
     3781 cccagcccgc cgagcgcgat gaggccggcg acgacccccg cgaggacccg cttccggcgg
     3841 cgtcgccggc gctcctcctc acgtcgctcg gccagccgct tcagctggcg ctcgcgcgtc
     3901 ctgctccgtc ccatggcgcc ggacaggtta ctcggctgag gcgagggcgg ccatctcgag
     3961 cagcagtcca tcgaggatgt cccgctccga gacgagacat cgctcgaacc cccaccgttc
     4021 catcgcctcc aacaggatct gcaccccggc gacgatgacg tcctcgcggc ccgggaccat
     4081 caccggcagg gcggcacgct cagccgtcgt catggcggcg agccgtcggg ccgtctcctc
     4141 aaccgtccga cggtggagcg tcgtgcgatg gatcgcgtcg gggtcgtacc gctcgagccc
     4201 gagcgcgatc gcctgcaccg tcgtggcggt cccggctacc gcgacgaggg tggccgcgga
     4261 gcccgcgggg atacgcgcga acgccggcgc cagtcctgcc gccgccgcgg cccgcatcgc
     4321 ctcgacctcg gtggcgtggg gtggatcgac cggtgccacg cgttccgtga tccggacgct
     4381 cccgacgtcg accgagatag cgcgctccgc ggatcccgtg ccgaccaccg cctccgtgct
     4441 gccgccgccg atgtcgagga cgaggtacgg cgtgggtgca tcgagccctc gggtcgcgcc
     4501 gaggaacgac agcgccgcct cgcgctcccc ggtcagcacc tcgggttccg cgcccgtcag
     4561 cgcccggacc cccgagaaga gctcctcccg ctcggccgcg tcccggacgg cgctcgtcgc
     4621 cccgaccttg atcctgcgca cgcccagggc cttcgcacgc cacgcgtacc agccgaggac
     4681 ctcgagggtc cgcgcgaggg acgcggggtc cagccggccg gtccggtcga cccctcggcc
     4741 caggcgcgtg atcaccatgt ctcgggcaag ctcgaccggc tcctcctcac gctggcggac
     4801 ctcggccacg aggagccgga tcgagttcgt tcccatatcc accgcggcga gccgctcgcc
     4861 gcggtggat
//
LOCUS       DATLGG010000066         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_51634, whole genome shotgun sequence.
ACCESSION   DATLGG010000066 DATLGG010000000
VERSION     DATLGG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51634"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VM638_01725"
     CDS             complement(<1..188)
                     /locus_tag="VM638_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HVM11183.1"
                     /translation="MNLDSFIEQYRPDWQRLESAVRQGSRSLADLRGDELDEAIRLYL
                     RVSSHLAEARARFGDPSLV"
     gene            200..931
                     /locus_tag="VM638_01730"
     CDS             200..931
                     /locus_tag="VM638_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVM11184.1"
                     /translation="MRDPGAMVTPEAVGLDADLAGVGSRLQAAVIDGLIQGGFVLVVL
                     LGLAAGDASSDVALITLLISVFVAIWIYPIVFEAAWAGQTPGKRVAKIRVRGTDGQPV
                     RFPGVLVRNLLRIVDLLPGVYAVGLVLILFTRRAQRLGDLAGGTVVVHEPVGAAPEAL
                     LLPPAEREERLARSIDVAGISPREYAVARSFLIRRDSMTPAARARVAGEIASRLRERV
                     GADASASAEEVVEAVVRAFRGGAGG"
     gene            948..1169
                     /locus_tag="VM638_01735"
     CDS             948..1169
                     /locus_tag="VM638_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HVM11185.1"
                     /translation="MFSRRRCGLCDAAREVLREAGAGREFTFEEVLIDDDDDLERRYG
                     LRVPVVLVDGEEAFEAEVDAEILRSLLGR"
     gene            1246..1935
                     /locus_tag="VM638_01740"
     CDS             1246..1935
                     /locus_tag="VM638_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HVM11186.1"
                     /translation="MRARPIPEATVGRLPLYLRALVEMAESGRTTVSSEALAEAAGVN
                     SAKVRKDLSYLGSYGTRGVGYDVAYLIHQVRRELGLTQDWPVLIAGVGNLGHALANYR
                     GFDERGFRVVALVDSDPDRLGEEVAGVRVRPTSEIPRIVREHDVAIGLICTPAAAAQD
                     VADAMVEAGVRSILNFAPAHITVPAAVSLRKVDLSIELQILAFYEQRKAALAEVRKGK
                     ALANAGLEAVT"
     gene            1937..3184
                     /locus_tag="VM638_01745"
     CDS             1937..3184
                     /locus_tag="VM638_01745"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HVM11187.1"
                     /translation="MALLTLGVSYRRAPIELLERLSFADDDLTKGYHHLSTLPSVREG
                     VIVSTCNRVEVYAAVSAYHEGFKDLKRFLAEARDAEVDDLAEPLYSHYEDHAVEHLFS
                     VAAGIDSMVVGEPQILQQVGAALKRARLEGTAGPVMDGLFRHAIRVGRRARSETAIGA
                     SPSAFVDAGSDLAAAHLGGLEGRSLLVVGAGKMSALAVRSLQARGIEGVRILNRTPAR
                     AQRLAAQTGAEAGSLDDLPGALGGADLIISSTASTAAVITRDLVREASPRRRFFLDLA
                     VPRDVDPEVRSIPDQAVADIADLRAGLKALPPNEEVDRVRGLIAEEVRRHAVRRRAAR
                     LAPLIRALQDRGEEVRQAELRRLRSRLAGLSERDREAVEALTRGIVNKLLHDPLVRLK
                     EPGRHDLGRAVADLFGLEPADDG"
     gene            3189..4064
                     /gene="hemC"
                     /locus_tag="VM638_01750"
     CDS             3189..4064
                     /gene="hemC"
                     /locus_tag="VM638_01750"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVM11188.1"
                     /translation="MPLRIGTRGSRLALAQAAEVAEMLGGETELVPIVTSGDRGASAE
                     GVAGGVKGLFVAEITEALARGEVDLAVHSAKDLPSEDPEGVVVAAVPQRALPYDVLIG
                     ADPGPGVRFGTSSLRRQAQLLRDHPDLEVVDLRGNVDTRLRRLEEGRCDGLVLAAAGL
                     ARLGLEPPGARPIPLDVMVPAPGQGALAVQAVEGSAAAEAAAAIDHPRSRAAFEAERD
                     LVRLLGGGCRLPLGAYAEWRDEAVRLMAIVIRPDGSDPLMAQVEAPDPAVAAAEAAEI
                     LRRGGAEDILREAEG"
BASE COUNT          517 a         1538 c         1553 g          589 t
ORIGIN      
        1 acgagggacg ggtcgccgaa ccgcgcccgc gcctccgcga ggtgggacga gacccgcagg
       61 tagagccgga tcgcctcgtc gagctcgtca ccgcggagat cggcgagcga cctcgaaccc
      121 tggcggaccg cgctttccag gcgctgccag tcggggcgat actgctcgat gaaggagtcg
      181 aggttcactc gaggcgagta tgcgcgaccc cggagcgatg gtcacacccg aagccgtcgg
      241 gctcgacgcc gacctggcgg gcgtcggctc gcgtctgcag gccgcggtga tcgacgggct
      301 gatccagggc ggattcgtcc tcgtcgtcct gctcgggctc gcggcgggcg atgcgagctc
      361 cgacgtggcc ctcatcaccc tgctgatctc ggtcttcgtg gcgatctgga tctacccgat
      421 cgtgttcgag gcggcctggg ccgggcagac gccggggaag cgcgtcgcga agatccgcgt
      481 gcggggcacc gacgggcagc cggtccgctt ccccggcgtg ctcgttcgga acctgctgcg
      541 catcgtcgac ctgctcccgg gtgtgtacgc ggtcggtctc gtgctcatcc tcttcacccg
      601 gcgcgcccag cggctgggcg acctcgccgg cgggacggtg gtcgtccacg aacccgtcgg
      661 agccgctccg gaggcgctcc tgctcccgcc ggcggagcgg gaggagcgcc tcgcccgctc
      721 gatcgacgtc gcggggatca gcccgcggga gtacgcggtg gcccggtcct tcctgatccg
      781 ccgcgattcg atgacgcctg cggccagggc gcgcgtcgcc ggcgagatcg cctcgcgcct
      841 gcgcgaacgc gtcggcgccg atgcctcggc ctccgccgag gaggtcgtcg aggccgtcgt
      901 gcgcgccttc cggggtggcg ccggtggatg atatcccgcg cgtgaggatg ttctcgcggc
      961 gccggtgcgg cctctgcgac gcggcccggg aggtcctgcg ggaggcgggg gccggccggg
     1021 agttcacctt cgaggaggtc ctgatcgacg acgatgacga cctcgagcgc cggtacggtc
     1081 tccgcgtccc ggtcgtcctc gtcgacggcg aggaggcctt cgaggcggag gtcgacgccg
     1141 agatcctgcg ctccctgctg ggccgctgag ccgagcgctt gcgcgtttgg gaagggaccg
     1201 ttcacgagcg tacaatcgga ccgtgacccc tggagcccgg ctcccgtgag agcccgtccg
     1261 atccccgagg ccaccgtcgg ccgcctgccg ctgtacctcc gtgccctcgt cgagatggcc
     1321 gagagcggcc gcacgaccgt ctcgtcggag gccctcgccg aggccgccgg ggtgaactcc
     1381 gccaaggtcc gcaaggacct cagctacctc gggtcctacg ggacgcgggg ggtcggctac
     1441 gacgtcgcgt acctgatcca ccaggtgcgc cgggagctcg gcctcaccca ggactggccg
     1501 gtcctgatcg ccggggtcgg gaacctcggt cacgcgctgg cgaactaccg cgggttcgac
     1561 gagcgcgggt tccgcgtcgt ggctctcgtc gacagcgacc cggaccgcct cggggaggag
     1621 gtcgcgggtg tccgtgtgcg accgacgagc gagatccccc ggatcgtccg cgagcacgat
     1681 gtggcgatcg gcttgatctg cacgccggcc gccgccgccc aggacgtcgc cgacgcgatg
     1741 gtcgaggcgg gcgtccgctc gatcctcaac ttcgcgccgg cccacatcac cgtgccggcc
     1801 gcggtctcgc tgcggaaggt ggacctgtcg atcgagctcc agatcctcgc cttctacgag
     1861 cagcggaagg cggcgctcgc cgaggtccgg aagggcaagg cgctggcgaa cgcgggcctc
     1921 gaggcggtga cctgaagtgg cgctgctcac gctcggcgtc tcctaccgtc gtgccccgat
     1981 cgagctgctc gagcggctct cgttcgccga cgacgatctg acgaaggggt accaccatct
     2041 ctccaccctg ccctccgtcc gcgagggcgt gatcgtctcg acctgcaacc gggtcgaggt
     2101 gtacgcggcg gtttccgcgt accacgaggg cttcaaggac ctcaagcggt tcctcgccga
     2161 agcgcgcgac gccgaggtcg acgatctcgc cgagccgctc tactcgcatt acgaggacca
     2221 cgcggtcgag cacctgttct ccgtggcggc cggcatcgac tcgatggtcg tcggcgagcc
     2281 gcagatcctc cagcaggtcg gggccgcgct caagcgtgcc cggctcgagg gaaccgccgg
     2341 ccccgtcatg gacggcctct tccgacacgc gatccgcgtc ggccggcgcg cgcgttcgga
     2401 gacggcgatc ggtgcgagcc cctccgcgtt cgtcgacgcg gggagcgacc tcgccgccgc
     2461 ccacctcggg gggttggagg gccgttccct gctcgtcgtc ggcgccggca agatgagcgc
     2521 gctcgcggtc cgctccctcc aggcccgggg catcgagggg gtgcggatcc tgaaccggac
     2581 gcccgcacgc gcacagcgcc tcgccgcaca gaccggcgcg gaggcgggat ccctcgacga
     2641 ccttcccgga gccctcggtg gggccgacct catcatctcc tcgaccgcct cgaccgcggc
     2701 cgtcatcacg cgcgacctcg tacgggaggc gtcgccgcgg cggcggttct tcctcgacct
     2761 ggcggtgccg cgcgacgtcg acccggaggt gcggtcgatc cccgatcagg ccgtcgccga
     2821 catcgcggac ctgcgggcgg gtctcaaggc gctcccgccg aacgaggagg tggaccgcgt
     2881 ccgcggcctc atcgccgagg aggtgcggcg ccacgccgtg cgacgccgag cggcccggtt
     2941 ggcgccgctg atccgggcgc tccaggaccg tggggaggag gtccgtcagg ccgagctccg
     3001 ccgcctccgc tcgcggctcg ccggcctctc cgagcgcgac cgtgaggccg tcgaggcgct
     3061 cacgcggggc atcgtgaaca agctgctcca cgacccgctc gtccggctga aggagccggg
     3121 ccgtcacgac ctcgggcggg cggtcgcgga tctgttcggg ctcgagcccg ccgacgacgg
     3181 ctgagcccat gccgctgcgg atcgggacgc gcgggtcccg cctcgccctc gctcaggcgg
     3241 cggaggtggc cgagatgctc gggggcgaga cggagctcgt ccccatcgtc acctctggtg
     3301 accgtggggc ctcggccgaa ggggtcgccg ggggcgtcaa gggcctcttc gtcgcggaga
     3361 tcaccgaggc cctcgcgcgc ggggaggtgg acctcgccgt ccactccgcg aaggacctcc
     3421 cctcggagga tccggagggg gtcgtcgtcg cggccgtgcc gcaacgcgcc ctgccctacg
     3481 acgtgctcat cggagcggac ccgggcccgg gcgtccggtt cggcacgtcg agcctgcgcc
     3541 gccaggccca gctcctgcgc gatcacccgg acctggaggt ggtcgacctg cgggggaacg
     3601 tcgacacgcg cctccggcgg ctcgaggagg gccggtgcga cgggctcgtg ctcgctgcgg
     3661 cgggactggc acggctgggc ctggagcccc ccggcgcgcg gcccatcccg ctcgacgtga
     3721 tggtgccggc tcccggccag ggagcgctcg cggtgcaggc ggtcgaagga tcggcggcgg
     3781 cggaggccgc cgcggcgatc gaccatcccc gttcgcgcgc cgcgttcgag gcggagcgcg
     3841 acctcgtccg gctcctcggc ggcggctgcc ggctcccgct cggcgcctac gccgaatggc
     3901 gcgacgaggc ggtccgcctg atggccatcg tgatccggcc ggatggctcc gacccgctga
     3961 tggcccaggt cgaagctccc gacccggccg tcgccgcggc cgaggccgcc gagatcctcc
     4021 gccgcggggg ggccgaagac atcctccgcg aggccgaggg atgacgcgcc cgctcgaggg
     4081 ccggaccgtc ctcgtgacgc gaccggagga gcgatccgac ccgatcgccc acctcctcgc
     4141 cgagcgcggc gccagcgtcc tgcgcgcgcc ggcgatcacg atcgagtcga tcgacgg
//
LOCUS       DATLGG010000067         4004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_37009, whole genome shotgun sequence.
ACCESSION   DATLGG010000067 DATLGG010000000
VERSION     DATLGG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37009"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VM638_01755"
     CDS             complement(<1..209)
                     /locus_tag="VM638_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HVM11189.1"
                     /translation="MYARVRGVALLGVEGHPVRVEAHVGRGLPALHLTGLPGATVQDA
                     RERVRPAVDNSGLEWPLRRVTVNLSP"
     gene            complement(270..2279)
                     /locus_tag="VM638_01760"
     CDS             complement(270..2279)
                     /locus_tag="VM638_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM11190.1"
                     /translation="MIEPAERRAGERAEGRPLFRLSGAGRVWVFNGLLAGMASWLYLS
                     FVVPLEPLAAPVSFPWWALALAFTFTELFVVHLPLRREAHSFSLSDITLVLGLFFVDP
                     ADLILAQLVGAGVALAIRRQSPMKLTFNLAHFCLEACVAVVVFHAIAGGDPIGPQGLL
                     AAFLATLTTTLLGALAVTAAISLAEGRFQHQTMSTGLGFGAVVTATDTCLALIAAEII
                     WWDASVAWLLLVPAVLLFSAYRLYTSQRQNHRTVEALYEATRHLQRSLRVESTMGTLL
                     EEARRMFRGEIAEVLLFPDVTGEKAVRTTLGPGDTPVSAEILLDPTEGVWARVASEGA
                     GVILPRPLPPSRLRTHLRARGIRDAMVAPLLTQGGVVGTLMVANRLGDVSTFEEEDLR
                     LLETLANHASVALENARLVGRLEDSLQHLTEMNRLKDDFVATVSHELRTPLTNIQGSI
                     KTLLRLPLDEADRRALLEAADRGGERLRHLIEDLLMASRIEAREVRPVVAPVSLDEVV
                     LAVALEFEDRAGGRLAVEGEEIPVIETDEGKVTQILSNLVDNALKHSEPGSDVRVRLF
                     REGRGIGIRVESSGTPIPPEQQDRIFERFVQVDQSSTRTVEGAGLGLYICRMLAEALG
                     GRVWLDRSDDTGTVFGIFIPMTPPERAVRDRNEISLPAASEPRRR"
     gene            complement(2293..>4004)
                     /locus_tag="VM638_01765"
     CDS             complement(2293..>4004)
                     /locus_tag="VM638_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVM11191.1"
                     /translation="ALEPAGSLVSVIVREAPAAGSAERAVEALGGEVGRPIGIIDGFA
                     ARVPEGRLDELRRASSVLSVTPNARVHLNHAIDGYNPADDAGSWFHTAKSVKADWAWS
                     NGFTGRGVDVALIDSGVVPVNGLTYPGKVINGPDLSFESQAEHLTYLDTFGHGTHMAG
                     IIAGRDDGKDPTQGNHDDFMGIAPGARLLSIKVANAMGAADISQVIAAIDWVVQHKDS
                     NGMNVRVLNLSFGTDGIQDYTLDPLTFAVEVAWHHGIVVVVSAGNEGFGNAKLNNPAY
                     DPYVVAVGASDTKGTFTTDDDVVPEWSSRGDGTRNPDLVAPGKSIVSLRSPESQADQS
                     HPGGRVGSRFFRGSGTSQSAAVVSGAAALLLERHPNATPDQIKRLLTSTATRMPVADP
                     VAQGSGVLNLKDAIRAPLPQHTQTWPRATGLGSLEAARGTGHVVDDGGELVGEVDIFG
                     TPWDPEEWTRRSWSRQSWSGGDWMGGDWSGSGWTRQSWSGMSWERRSWSRQSWSRQSW
                     SRQSWSADVWDRQSWDRQSWSRRSWSGQAWSATNWSRQSWSRQSWSSAAWGGEPVAAE
                     STA"
BASE COUNT          599 a         1440 c         1415 g          550 t
ORIGIN      
        1 gggctgaggt tgaccgtgac gcggcgcagc ggccactcga gcccggagtt atcgacggcc
       61 ggccggaccc gctcgcgcgc gtcctgcacg gtcgccccgg ggagacccgt caggtgcagc
      121 gccgggaggc cccgcccgac gtgcgcctcg acccggacgg gatggccctc cacccccaag
      181 agcgcgaccc cgcggacccg tgcgtacacg gacgggaggc tagcgagggg tgctgacacg
      241 aacctcgcgg cgaccgctgc gccgcaggtt cagcggcggc gaggctcgga cgccgcgggc
      301 agtgagatct cgttgcggtc gcggaccgcc cgctcgggcg gcgtcatcgg gatgaagatg
      361 ccgaagacgg tgcccgtgtc gtcggagcga tccagccaga cccgtccccc caacgcctcg
      421 gccaacatcc ggcagatgta gagcccgagc ccggcccctt ccacggtcct ggtcgagctc
      481 tggtcgacct ggacgaaccg ctcgaagatc cgatcctgct gctcgggcgg gatcggcgtg
      541 ccgctgctct cgacccggat cccgatccct cgtccctcgc ggaagagccg cacgcgcacg
      601 tcgctccccg gctccgagtg cttgagggcg ttgtccacca ggttcgagag gatctgcgtg
      661 accttcccct cgtccgtctc gatgaccggt atctcctccc cctcgacggc gagacgcccg
      721 ccggcccgat cctcgaactc gagcgcgacc gccagcacca cctcgtcgag ggagaccggg
      781 gcgacgacgg gacggacctc gcgtgcctcg atccgggacg ccatgagcag gtcctcgatc
      841 aggtggcgga gccgctcgcc gccgcggtcg gcggcctcga gcagcgcgcg ccggtcggcc
      901 tcgtcgagcg ggaggcgcag gagggtcttg atgctgccct ggatgttcgt gaggggcgtg
      961 cggagctcgt gggagaccgt cgcgacgaag tcgtccttca gccggttcat ctcggtcagg
     1021 tgctggagcg agtcctccag gcgaccgacg agccgggcgt tctcgagcgc gacgctcgcg
     1081 tggttggcga gggtctccag gagccgaagg tcctcctcct cgaacgtgct gacgtcgccg
     1141 aggcggttcg cgaccatgag ggtgcccacg accccgccct gggtgaggag cggcgcgacc
     1201 atcgcgtccc ggatcccccg cgcacggaga tgggtccgca ggcgggacgg cgggagcggc
     1261 cgcggcagga tgaccccggc gccctccgac gcgacgcggg cccagacccc ctccgtcgga
     1321 tcgagcagga tctccgcgct tacgggggta tcgcccggac cgagcgtcgt gcggacggcc
     1381 ttctcgccgg tgacgtcggg gaacaggagg acctccgcga tctcgccgcg gaacatccgg
     1441 cgcgcctcct cgagcagcgt ccccatcgtc gactctacgc ggagggatcg ctggagatgc
     1501 cgcgtggcct cgtacagggc ctcgaccgtc cggtggttct gacgctgcga cgtgtacaga
     1561 cggtaggcgg agaagagcag gaccgcggga acgagcagca accaggccac ggaggcgtcc
     1621 caccagatga tctcggccgc gatcagcgcg aggcacgtgt cggtcgcggt cacgacggcg
     1681 ccgaacccca gacccgtcga catcgtctgg tgctggaagc gcccctccgc cagggagatc
     1741 gcggcggtca ccgcgagggc tccgaggagg gtcgtcgtga gcgtggcgag gaaggcggcc
     1801 aggagtccct ggggcccgat cgggtccccc ccggcgatcg cgtggaagac gacgacggcc
     1861 acgcaggcct cgagacagaa gtgggcgagg ttgaacgtga gcttcatcgg tgactgccgg
     1921 cggatcgcga gcgccacccc ggcgccgacc agctgggcga ggatgaggtc cgcgggatcg
     1981 acgaagaaca gtccgaggac gagcgtgatg tcgctgagcg agaagctgtg ggcttcgcgt
     2041 cgcagcggca ggtggacgac gaagagctcc gtgaacgtga acgcgagcgc gagcgcccac
     2101 caggggaagc tgaccggcgc ggccagcggc tccagcggga cgacgaacga gagatagagc
     2161 cagctggcca tccccgcgag cagcccattg aacacccaga cccgtccggc cccggacagc
     2221 cggaagagcg gacggccctc cgcccgctct cccgcccgac gctccgccgg ttcgatcaac
     2281 gacgcgcccc gctcatgcgg tggactccgc ggccaccggt tcgccgcccc aggcggcgct
     2341 cgaccacgac tgacggctcc acgactgacg gctccagttc gtcgcgctcc acgcctgacc
     2401 gctccaggat cggcggctcc aggactgccg atcccaggac tggcggtccc agacgtccgc
     2461 gctccatgac tggcggctcc acgactgccg cgaccacgac tggcggctcc aggaacgccg
     2521 ctcccagctc atcccgctcc aggactgacg cgtccaccca ctgccgctcc agtccccgcc
     2581 catccagtcc ccgccgctcc aggactggcg gctccaggac cgacgcgtcc actcctcggg
     2641 gtcccacggc gtgccgaaga tgtcgacctc gcccaccagc tccccgccgt cgtcgacgac
     2701 gtgaccggta ccacgcgcgg cctcgaggga cccgagaccc gtggcgcgcg gccaggtctg
     2761 ggtgtgctgg ggcagcgggg cgcggatcgc gtccttcagg ttcagcacgc cgctcccctg
     2821 tgcgaccgga tcggcgaccg gcatccgggt ggcggtcgag gtcagcagtc gcttgatctg
     2881 gtcgggcgtc gcgttcgggt gccgctcgag gagcagggcg gcggcgccgg agacgaccgc
     2941 tgccgactgc gaggttccgc tcccgcggaa gaaccgcgaa ccgacgcgcc cgccgggatg
     3001 gctctggtcc gcctgcgact cggggctgcg caggctcacg atcgacttgc ccggtgcgac
     3061 gaggtcgggg ttgcgggtcc cgtcgccgcg gctggaccac tcgggtacga catcgtcgtc
     3121 ggtcgtgaag gtacccttcg tgtccgaagc gccgaccgcg accacgtacg ggtcgtacgc
     3181 cgggttgttc agcttcgcgt tgccgaaccc ctcgttcccc gccgagacga cgaccacgat
     3241 cccgtggtgc cacgcgacct ccaccgcgaa cgtcaggggg tcgagcgtgt agtcctggat
     3301 cccgtcggtg ccgaaggaca ggttcagcac acgcacgttc atcccgttcg agtccttgtg
     3361 ctgcaccacc cagtcgatcg cggcgatgac ctgggagatg tcggccgcgc ccatcgcgtt
     3421 ggcgaccttg atgctgagga ggcgggcgcc aggagcgatc cccatgaagt cgtcgtggtt
     3481 gccctgggtc gggtccttcc cgtcgtcgcg tccggcgatg atcccggcca tgtgggtccc
     3541 gtgaccgaag gtgtcgaggt aggtcaggtg ctcggcctgc gactcgaagg acagatcggg
     3601 gccgttgatc accttgccgg gataggtgag accgttcacg gggacgaccc cggaatcgat
     3661 cagggcgacg tccaccccgc ggcccgtgaa cccgttcgac caggcccagt cggccttgac
     3721 cgacttcgcg gtgtggaacc acgacccggc gtcgtccgcc gggttgtaac cgtcgatcgc
     3781 gtggttgagg tggacccgcg cgttcggggt caccgagagc accgacgatg cgcgccggag
     3841 ctcgtcgaga cgcccctcgg ggacgcgcgc cgcgaacccg tcgatgatcc cgatcggacg
     3901 gccgacctcg ccaccgagag cctccaccgc gcgctccgcc gagccggcag ccggcgcctc
     3961 tcgcacgatc acggacacca gcgagcccgc cggctcgagg gccg
//
LOCUS       DATLGG010000068         5852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_161324, whole genome shotgun sequence.
ACCESSION   DATLGG010000068 DATLGG010000000
VERSION     DATLGG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161324"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..305
                     /locus_tag="VM638_01770"
     CDS             168..305
                     /locus_tag="VM638_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11192.1"
                     /translation="MRRGTLIAIIALFLLIAIAATIQLGYGSRGDRDLPGPGAPSTPA
                     G"
     gene            complement(310..1107)
                     /locus_tag="VM638_01775"
                     /pseudo
     CDS             complement(310..1107)
                     /locus_tag="VM638_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(1125..2096)
                     /locus_tag="VM638_01780"
     CDS             complement(1125..2096)
                     /locus_tag="VM638_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVM11193.1"
                     /translation="MGQRVLVTGAAGHLAAALARRLADDPAVEEVVAVDRTEPEHPLG
                     GARFVPADIRTPVFLSILQTSGADTVVHLDLLATPAGAGGRGRMKEINVIGAMQLFAA
                     CQRAQSVRKVVVKSSTAVYGSDAEMPAVFTEEMAARGQVRGGYAKDAVEVEGYARDLT
                     RRREGVALTILRFANFMGPRIQSSLTRYFSLPVVPTPIGYDPRLELIHEDDALEVLER
                     VTREDHPGVFNVSADGIVYLSQALRLLGKPPLPVLPPLGRPLASVLTRLRLVDVAFDQ
                     LPLLLHGRVTDNARLKRTLGWEPKFDTRAALLDVAAAERRRIEEALS"
     gene            complement(2102..2197)
                     /locus_tag="VM638_01785"
     CDS             complement(2102..2197)
                     /locus_tag="VM638_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HVM11194.1"
                     /translation="MPSLVKKRRKKMRKKKHKKLLKKTRWQRRQK"
     gene            complement(2208..3098)
                     /locus_tag="VM638_01790"
     CDS             complement(2208..3098)
                     /locus_tag="VM638_01790"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HVM11195.1"
                     /translation="MDPHEDLVRRAVERRGLTLLLGGLDTGKTTLARSIAAAGIEAGL
                     PVGLVDADVGQSTVGPPTTVGLRMCRRPSDLEPTSLTKADAMAFVGSTSPQGHVLPLA
                     VGAALMVQRARTEGAEMIVVDTTGLVTGIFAQVLKYHKVTLLQPDLVVGLERGAELEP
                     LLGVIRRFAAAEVVTLPVHPEVVPTSVERRAAHREESMRRYFDEPLHRWRVKPTVFMP
                     ALPALFDLSALDRLLVGLSDGKGGCPGIGYLEHVAEDGALRLVSPAAEGPKALILGSM
                     RLEDDFRARRMDLRNLFGSD"
     gene            complement(3147..3347)
                     /locus_tag="VM638_01795"
     CDS             complement(3147..3347)
                     /locus_tag="VM638_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM11196.1"
                     /translation="MAQQASFREARFLTVNEVARFLRVSKMTVYRLIKQGDLPAVQIG
                     RGYRVREEDVHRYLDARYTEAG"
     gene            3437..4144
                     /locus_tag="VM638_01800"
     CDS             3437..4144
                     /locus_tag="VM638_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVM11197.1"
                     /translation="MTGTLYLGTSGFAYPEWKGVFYPPETKGEEMLPFYASRFRSVEV
                     NYTFRRTPAETTLATWLERTPPGFRFTLKAHQRITHTRRLRDAEEPLGVFLERARRLG
                     DRLGPTLFQLPPTFEHDPQALEAFLTLLPDDLRFAFEFRHPSWEAARPVLAERGVAWC
                     VAETDEGPAGAMAWEPFGYLRLRKESYADEELVVWAERIRDALAAGRDVHCYLKHEEK
                     GTGPRYAMKLKELIEGG"
     gene            complement(4148..4963)
                     /locus_tag="VM638_01805"
     CDS             complement(4148..4963)
                     /locus_tag="VM638_01805"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HVM11198.1"
                     /translation="MASKPQSSRVLESDPWARGSYTREEIRRALLEGGMAGVVSHDRP
                     NVVWKLGKLCDGDPSSQFGIRGVDVDTFEQVIAIMAEASGFDPDPSVRHGLVRVDAGK
                     VIDRLERVGDRLALAAQGGERVLLATGHPNGLPLLYAATGRLLEKHGATLITPADGRV
                     IDEGRKRRVIRYEDRVGMLTNGVKGIHTHSPAGMRRMLEEEVPDLVFADHGFAGAAIE
                     IVSTPASIADVNDPALVVAEAKGRGGPVVVMDDNVEPEDYWPCFQVIASRFPD"
     gene            4995..>5852
                     /locus_tag="VM638_01810"
     CDS             4995..>5852
                     /locus_tag="VM638_01810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HVM11199.1"
                     /translation="MPELEGRVALVTGGSKGIGRAIALRFARAGADVAIAARGAEALE
                     RTAVEIRETGRRALAVPTDVTDADQVRSLVERTAADLGTIDVLVNNAGAAPFLATIDQ
                     IREDGFEKYFRVTFTSAFHCTKAVAPIFLAKGSGAVVNVASVAAFIASPGLTYYGAAK
                     AALINLTKTVAREWASSGIRVNAIAPGWIETEMNAVGRGNPDWYERVRSAIPLGRWGT
                     AEEIAEVALFLASDAASFITGAVIVADGGQTTANLVGQHREQELRQAKDRQVVRRRHP
                     LEVREREVRE"
BASE COUNT          867 a         2095 c         2062 g          828 t
ORIGIN      
        1 cctctactcc gcgttcatcg acgcgcagct ccaggaggag gcgatccagg cgggcgtcgc
       61 cgcgtgcgtg ccgaaaggct ccggggtgga gtcgctcgcc accgagatca gcgccctcgc
      121 gatggacctc ggcgggaacg acgacgggtc gaggcgggag tgagccgatg cggcgcggca
      181 cgctgatcgc gatcatcgcc ctgttcctgc tcatcgcgat cgccgccacg atccagctcg
      241 gctacggcag ccggggcgac cgcgacctcc ccggccccgg cgcaccgagc acccccgccg
      301 gctgagccct cagaacgccg agccgcgggc ggcgaggttg cggtcgagca tcgcctggat
      361 cgtgccgcgg atctgctcgg tgagctcgaa gacggtgagg ccgtcgcgcc acgcctcggg
      421 cccgtggccg tcggtgggga tcggctcacc gaactcgatc agccacttcg acggcagcgg
      481 gatcatcccg agcgggccga gccacgggaa gaacggcgtg atcggaacgt aggggagccc
      541 gagggcgcgg gcgagcgggc gcgcctgggc gatcatcgga tagatctcct cggcgccgac
      601 gatcgagacc ggcacgatcg gcacgcccgt gcgcagggcc agctcgatga agccaccccg
      661 cccgaagcgc tggagcttgt accggtcacg gaagtgcttg ccgacgccct tgaacccctc
      721 ggggaagacc ccgacgagct caccgcgctc gagcaggcgc agggcgtcct cttcgcatgc
      781 gagcgtgttc cccgatttgc gcgcgagctc cccgatcacc ggcatccgca gggccaggtc
      841 ggccgccagc agtcggacgt ggcgccgagc cgggtggtgg tcgagcaccc cgaacttcat
      901 cacgaccgcg tcgaacggga cggccccgga gtggttcgcg acgagcagcg ccgcgccctc
      961 ggaggggatc cgctcgatgc ctcgccactc gacgcgccac cagtcccggt acagcggccg
     1021 ggcgatccgc agcagcacct cctcggtcag ctccgggtcg aacccgaact catcgacctc
     1081 gtactccccc gagagccgcc ggcggaggat gtcgacgacg tcgctcacga gagcgcttcc
     1141 tcgatccgtc gtcgctccgc cgcggcgacg tcgagcagcg ccgcccgggt gtcgaacttc
     1201 ggctcccacc cgagcgtccg cttcagccgg gcgttgtccg tgacgcgtcc gtgcaggagg
     1261 agcggcaact ggtcgaaggc tacgtccacg agtcgcagcc gggtgaggac cgacgcgagc
     1321 ggccggccca gcggaggcag gaccggcagc ggcggtttcc cgaggagccg gagcgcctgc
     1381 gagaggtaga cgatcccgtc cgcggagacg ttgaagactc ccgggtgatc ctcgcgcgtc
     1441 acgcgctcca gcacctcgag cgcatcgtcc tcgtggatca gctcgagccg cggatcgtag
     1501 ccgatgggcg tcggcacgac gggtagcgag aagtaccgcg tgagcgagct ctggatccgc
     1561 ggacccatga agttcgcgaa gcgcaggatc gtgagcgcga ccccctcgcg gcgtcgcgtg
     1621 aggtcccgcg cgtagccctc gacctcgacg gcgtccttcg cgtagccgcc ccgcacctgc
     1681 ccgcgcgcgg ccatctcctc ggtgaacacg gccggcatct cggcatcgct cccgtagacg
     1741 gcggtcgaag acttcacgac gaccttgcgg acgctctggg cacgctggca agcggcgaag
     1801 agctgcatcg cgccgatcac gttgatctcc ttcatccgtc cccgcccgcc ggctcccgcc
     1861 ggcgtggcga ggaggtcgag gtggacgacc gtgtcggcgc cgctcgtctg caggatcgac
     1921 aggaacaccg gggtccggat gtcggcgggg acgaagcggg cgccgccgag cgggtgctcc
     1981 ggctcggtgc ggtcgaccgc aacgacctcc tccaccgcgg ggtcgtcggc gaggcggcgc
     2041 gcgagcgcgg cggcgaggtg accggcggct cccgtgacga ggacccgctg ccccatcgct
     2101 cctacttctg gcggcgctgc cagcgggtct tcttcaggag cttcttgtgc ttcttcttcc
     2161 gcatcttctt gcggcgcttc ttgacgagtg acggcacgaa cgtcctttca gtcggatccg
     2221 aacaggttgc gaaggtccat gcgcctggcc cggaagtcat cctccaagcg catcgaacca
     2281 aggataagcg ctttcggccc ctcggcggcg ggcgagacca accgcagggc gccgtcctcc
     2341 gcgacgtgct cgaggtaccc gatccccggg cacccgccct tgccgtcgga gagcccgacg
     2401 aggaggcggt cgagggccga gagatcgaac agcgccggca gcgccggcat gaacaccgtc
     2461 ggcttcaccc gccagcggtg aaggggctcg tcgaagtagc gccgcatcga ttcctcacga
     2521 tgggcggccc ggcgctcgac cgaggtcggg accacctcgg gatgcaccgg cagcgtcacg
     2581 acctcggcgg cggcgaaccg gcggatcaca ccgagcagcg gctcgagctc cgcccctcgc
     2641 tcgagcccca cgacgaggtc tggctggagc agggtgacct tgtggtactt caggacctgc
     2701 gcgaagatcc cggtgacgag cccggtcgtg tcgacgacga tcatctccgc accctcggtg
     2761 cgcgcgcgct ggaccatgag cgcggcgccg accgccagcg ggaggacgtg accctgcggc
     2821 gacgtcgatc ccacgaacgc catcgcgtcc gccttggtca gcgacgtcgg ctcgaggtcg
     2881 ctcggccgcc ggcacatccg cagacccacg gtcgtcggtg ggccgaccgt cgactgaccc
     2941 acgtcggcgt cgacgaggcc caccgggagt cccgcctcga tgcccgcggc agcgatgctg
     3001 cgcgcgaggg tggtcttccc cgtgtcgagc ccgccgagca ggagggtcag accgcgccgt
     3061 tcgacggccc ggcggaccag gtcttcgtgt gggtccatct cgcctccggt ctcccggagg
     3121 ctcggctcct cgccgtggtg aggggtctag ccggcttcgg tgtacctcgc gtcgaggtat
     3181 cggtgaacgt cttcctcccg gacccggtac ccccggccga tctgcacggc ggggaggtcc
     3241 ccctgcttga tgagtcggta cacggtcatc ttggacaccc gcaggaagcg ggcgacctca
     3301 ttcacggtca ggaaacgagc ctctcggaac gatgcttgct gggccacgct ggggcgattc
     3361 tagcggagga aggggtggcg cgaacagccc cgcaaccggc gcgaaggtga cgagacgggc
     3421 atgggaggat acgcgggtga cgggaaccct ctacctgggc acgtccgggt tcgcctatcc
     3481 cgagtggaaa ggcgtcttct accccccgga gacgaagggc gaagagatgc tccccttcta
     3541 cgcctcgagg ttccgctcgg tcgaggtgaa ctacaccttc cgccgcacgc ccgccgagac
     3601 gacgctcgcc acctggctgg agcgaacgcc gccgggattc cgcttcacgc tgaaggcgca
     3661 ccagcggatc acccacacgc gtcggctccg ggatgcggag gagccgctcg gcgtcttcct
     3721 cgagcgcgcc cggaggctgg gcgaccggct cggcccgacg ctcttccagc tccccccgac
     3781 cttcgagcac gacccgcagg cgctggaggc cttcctcacc ctcctccccg acgacctccg
     3841 cttcgcgttc gagttccggc atccctcctg ggaggccgcc cggcccgtac tcgccgagcg
     3901 gggggtcgcc tggtgcgtcg cggagaccga cgaaggcccc gccggagcga tggcctggga
     3961 gccgttcggg tacctccgcc tgcggaagga gtcctacgcg gacgaggagc tcgtcgtctg
     4021 ggcggagcgg atccgcgacg cgctggcggc ggggcgcgac gtccactgct acctcaagca
     4081 cgaggagaag gggacgggcc cgcgctacgc gatgaagctc aaggagctga tcgaaggcgg
     4141 ctgagtctca gtcggggaac cgcgaggcga tgacctggaa gcagggccag tagtcctcgg
     4201 gctcgacgtt gtcgtccatc accacgacgg ggccgccgcg tcccttcgcc tcggcgacca
     4261 cgagcgccgg gtcgttcacg tccgcgatcg aggccggcgt ggacacgatc tcgatcgcgg
     4321 cgcctgcgaa gccgtggtcc gcgaacacga ggtcggggac ctcctcctcg agcatccgcc
     4381 gcatccccgc gggactgtgg gtgtggatcc ccttgacgcc gttcgtcagc atccccaccc
     4441 ggtcctcgta gcggatcacg cgccgcttcc tgccctcgtc gatcacgcgg ccgtcggccg
     4501 gtgtgatgag cgtcgcgccg tgcttctcga ggagccgccc ggtggccgca tagagcagcg
     4561 ggagtccgtt cgggtggccg gtcgcgagca ggacccgctc cccaccctgc gccgcgagcg
     4621 ccaggcggtc cccgacgcgc tccaggcggt cgatcacctt cccggcgtcg acccggacga
     4681 ggccgtggcg cacgctgggg tccgggtcga agccgctcgc ctcggccatg atcgcgatga
     4741 cctgctcgaa cgtgtcgacg tcgaccccgc ggatcccgaa ctgcgagctc ggatcgccgt
     4801 cgcagagctt gcccagcttc cagacgacgt tcggccggtc gtgggagacg acccccgcca
     4861 tgccgccctc gagcagcgcg cgccggatct cctcgcgggt gtaggacccg cgcgcccacg
     4921 ggtcggattc gaggacccgt gagctttgtg gtttgctggc catcgaaccc aatcgtaggc
     4981 gaggaggtgt cctggtgccc gagctcgagg ggcgggtcgc gctggtcacc ggcggttcga
     5041 aggggatcgg ccgggcgatc gcgctgcgct tcgcccgcgc cggcgccgac gtggcgatcg
     5101 ccgcgcgcgg ggccgaggcc ctggagcgga ccgccgtgga gatccgcgag accggccgcc
     5161 gggccctggc ggtgcccacc gacgtcaccg acgccgacca ggtgcgatcc ctcgtcgagc
     5221 gaaccgccgc cgacctcggc acgatcgacg tcctcgtgaa caacgccggc gcggcgcctt
     5281 tcctcgcgac gatcgaccag atccgcgagg acggcttcga gaagtacttc cgcgtgacgt
     5341 tcacgagcgc cttccactgc acgaaggccg tcgcgccgat cttcctggcg aagggctcgg
     5401 gcgccgtcgt gaacgtcgcc tcggtcgcgg cgttcatcgc gagcccgggg ctcacctact
     5461 acggcgcggc gaaagcggcg ctcatcaacc tcacgaagac ggtggcccgc gagtgggcgt
     5521 cgagcgggat ccgcgtgaac gcgatcgcgc cgggctggat cgagaccgag atgaacgcgg
     5581 tcgggcgcgg gaaccccgac tggtacgagc gggtgcgctc ggccatcccg ctcggacgct
     5641 ggggcaccgc ggaggagatc gccgaggtcg cgctcttcct cgcgtccgac gccgcctcgt
     5701 tcatcaccgg cgccgtgatc gtcgccgacg gcgggcagac gaccgcgaac ctcgtcggcc
     5761 agcatcggga gcaggagctt cgtcaggcga aggaccgaca ggtagttcgt cgccgtcacc
     5821 cgctcgaggt gcgcgagaga gaggtccgcg aa
//
LOCUS       DATLGG010000069         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_161464, whole genome shotgun sequence.
ACCESSION   DATLGG010000069 DATLGG010000000
VERSION     DATLGG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161464"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VM638_01815"
     CDS             complement(<1..237)
                     /locus_tag="VM638_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11200.1"
                     /translation="MEMEKAGGKRDRSRYVTLPVMEEKGFVVLKPYEGQMPEPGEWEN
                     LEYVDWKSGGDTNFAILASANGDHDPRGFWEHGKP"
     gene            complement(328..>2019)
                     /locus_tag="VM638_01820"
     CDS             complement(328..>2019)
                     /locus_tag="VM638_01820"
                     /inference="COORDINATES: protein motif:HMM:NF039608.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11201.1"
                     /translation="VAEGSGSDLSLTVADGEQVTCTITNIRKTGKIEVVKALEPADDP
                     GEFELLIQDGEGSMVGSTGPVGHHGSTGSVELPTGTYTVAERAGGASSLGDYHSDISC
                     DPRQGDAGALALSEGDATVVLGSGDDVVCTITNTRKTGRIEVVKVLEPAGDPGEFDLF
                     IEDSEGSTVGSTTPVGHLGSTGPVQIPTGTYTVGERAAPGTSLDDYGRGIVCGPREGD
                     GAGALGDSGDGPVQDEIVTVADGDDILCTITNTRLGSITVTTETDPADARSANFTYSG
                     TGTDMPQHLTLDTAADDDTNPSTFMVAGLLPGSYSISATDVPAGWTLQGLECVGGDGT
                     SADAGTGVASIELSGGEDVECTFSHGRTPLSGTVVKTNDADLQGAFSHQEVASAAGQS
                     VRFQLVITNTSPVPVTIEAIKDTFAGSTVDVPECLDASAANAVGMELSRGGSDGDAVT
                     CRFTLARYAPASNSQLLNTAGVVLRDEFSSFSVTDTSVVTTPELPAREDDDDDDTRDS
                     GNGIDNDARPALLAEEPQRPGGLALTGVRLWFLLLFSLALMVAGFAAREKAGSEA"
BASE COUNT          296 a          726 c          654 g          343 t
ORIGIN      
        1 gggcttcccg tgctcccaga agccgcgcgg gtcgtggtcg ccgttcgccg aggcgaggat
       61 cgcgaagttc gtgtctccgc cggacttcca gtcgacgtac tcgaggttct cccactctcc
      121 gggctccggc atctgcccct cgtagggctt cagcacgacg aaccccttct cctccatgac
      181 ggggagggtc acgtacctcg agcggtccct cttgccgccg gccttctcca tctccatgcg
      241 aacgtcctcc tcgggttgcc ttcggtgccc tcatcgtagc cgaggtggcg ctgcggcagc
      301 cacgctgccg cagccgcccc cgctcaccta ggcctcgcta cccgctttct cgcgcgcagc
      361 gaaaccagcc accatcaggg ccagcgaaaa gaggagcagg aaccacaggc gcactccggt
      421 cagcgcgagg cccccggggc gctggggctc ctctgccagg agcgccggtc gcgcatcgtt
      481 gtcgatcccg tttcccgagt cgcgcgtgtc gtcatcatcg tcatcctccc tcgccggcag
      541 ctccggggtg gtgaccacag aggtgtcggt gacggagaag ctggagaact cgtctcgaag
      601 gaccacgcca gcggtgttca gcagctggct gttcgacgcg ggagcgtacc gggccagggt
      661 gaaccggcag gtgaccgcgt cgccgtcgga cccaccccgg gaaagctcca tcccgacggc
      721 attcgccgcc gatgcatcga ggcactccgg gacatcgacg gtggatcccg cgaacgtgtc
      781 cttgatcgcc tcgatcgtca cgggcacggg actcgtgttg gtgatcacga gctggaacct
      841 gacggactga ccggccgccg acgccacctc ctgatgggag aaggcgccct ggagatcggc
      901 gtcgttcgtc ttgacgacgg ttcccgacag aggcgtgcgg ccgtgcgaga aggtgcactc
      961 gacgtcctcg ccaccactca gctcgatgga cgccacgccc gtccccgcat cggccgacgt
     1021 cccgtctccg ccgacgcact cgaggccttg cagcgtccag ccggcgggaa catcggtcgc
     1081 ggagatcgag tagctgccgg gaagcagccc ggccaccatg aaggtggagg gattggtgtc
     1141 gtcgtccgcg gccgtatcga gggtcaggtg ctgcggcatg tcggtgccgg tgcccgagta
     1201 cgtgaagttc gccgaccgcg cgtctgccgg atccgtctcg gtcgtgacgg tgatcgaccc
     1261 gagccgcgtg ttcgtgatcg tgcagaggat gtcgtcgccg tccgccacgg tcacgatctc
     1321 gtcctggacg ggcccgtcac cgctgtcgcc cagcgcgcca gccccatcac cctcgcgcgg
     1381 gccgcagacg atcccacgcc cgtagtcgtc gaggctcgtc ccgggagccg cccgctcgcc
     1441 cacggtgtag gtccccgtcg ggatctgtac gggacccgtc gagcccagat gaccgacggg
     1501 ggtggtcgat cccaccgtcg agccttcgct gtcctcgatg aacaggtcga actcgccggg
     1561 gtcaccggcg ggctccagca ccttcacgac ttcgatccgc ccggtcttcc gggtgttggt
     1621 gatcgtgcag acgacgtcat ccccggatcc caacacgacg gtggcatcgc cctcggagag
     1681 cgcgagcgcc cccgcgtccc cctgccgtgg gtcgcatgag atgtcggagt ggtagtcgcc
     1741 gagcgagctt gccccgccgg cacgctcggc gacggtgtag gtcccggtgg gcagctcgac
     1801 ggagccggtc gatccgtgat gcccgacggg gcccgtcgat cccaccatcg agccttcgcc
     1861 gtcttggatc agcagctcga actcgcccgg gtcatccgcc ggctcgagcg ccttcacgac
     1921 ctcgatcttc ccggtcttcc ggatgttcgt gatcgtgcag gtaacctgct cgccatccgc
     1981 aaccgtcagc gaaagatcgg atcccgaacc ttccgcgac
//
LOCUS       DATLGG010000070         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_21377, whole genome shotgun sequence.
ACCESSION   DATLGG010000070 DATLGG010000000
VERSION     DATLGG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21377"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VM638_01825"
     CDS             complement(<1..1166)
                     /locus_tag="VM638_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVM11202.1"
                     /translation="MATVAGRLKRLILGRAIASEKAEHHLLPKTLALPVFASDPLSSV
                     AYATEEMMLALVVGGAAALTLMLPIGGAIALVLLVVVTSYRQTVRAYPQGGGSYIVAR
                     ENLGTIPGLLAAGAILIGYVVTASVSATAGAVAVTSAVPALAEHKITLAMGFIALISL
                     ANLRGVRESGMLFAVPTYGFVASIYVTVGAGLIRCMDVCPRAETADLPLHEESTLNLF
                     LILLAFTSGSSALTGVEAVADGVQAFRRPRARNAAATLAMMAAISVTMFLGVTLMASL
                     LEVRVTEEIAGARSVLAQIGETIFGRSVMFFVLQAFTAMILIAAANTAYQDFPRLSAI
                     LARDRFMPGQFRNRGDRLVFSNGILVLAVSSALLVWLFNAELTRLIAVYLVGVFT"
     gene            1240..1896
                     /locus_tag="VM638_01830"
     CDS             1240..1896
                     /locus_tag="VM638_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HVM11203.1"
                     /translation="MHVIIMGCGRVGAELTLQLSKAGHSVAVIDKRASAFDRLPPGFQ
                     ANRVVGYGFDREVLAKAGIDQADAFIAVSNGDNSNILSARVAREHYKVPKVIARIYDP
                     RRAEIYERLNIPTVATVIWAAKQIQYLLFHGREELKETFAGGTLMRLQVKVPSHLVGK
                     PIDSVVETGKVTVIGVDRGGGGFIPTGGSTFQEGDVAHFIVHKDAMDRYDFLMEPEAE
                     "
     gene            1898..2563
                     /locus_tag="VM638_01835"
     CDS             1898..2563
                     /locus_tag="VM638_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HVM11204.1"
                     /translation="MRIVVTGAGKIGRHLANDLQERGHEVTLVEQDSGEMERARGEAV
                     EGLTLVLGDACEPWVLDAAELRTCDVVVAATGDDEDNLVTSLLAKQEYGVPRVIARVN
                     HPNNEWLFTEQWGVDVAVSPPHVLTALVEEAVTTGDLVRLLKLEGGEVSLVEVKLTES
                     SHMVGRPLYELRLPQDAAVVAVLRGGHVVIPQPETQLAAGDEVIAIATPENEDQIRTA
                     LMG"
     gene            complement(2596..2955)
                     /locus_tag="VM638_01840"
     CDS             complement(2596..2955)
                     /locus_tag="VM638_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HVM11205.1"
                     /translation="MSWFRRMRERMGQSDEERLREETRQWAAEVAGATPIGSCERRSR
                     CRIAGVVRRMTIRPGEESLEALVTDGTGEVVAVWTGRSHIPGVGLGTRVLLEGMVVEE
                     KGRLRVVNPGYEFVGSH"
     gene            complement(2966..>4629)
                     /locus_tag="VM638_01845"
     CDS             complement(2966..>4629)
                     /locus_tag="VM638_01845"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HVM11206.1"
                     /translation="LADEVGADVGADLVGKVEKNIPEDDPRNAAVIADNVGDNVGDCA
                     GMAADLFESYEVTLVASIILGAAAFAGSPQGAIVGVMFPLFVRAIGVITSIIGIYSVT
                     PRSEEEHGMRSINRGFFFSAVLSAAAVYFVATGYLDDIRGFWAVLIGLLLASVIQLLT
                     EHFTSTTRRPVKEVAESSLTGPATTILSGFSVGLESTVWAILVIGGAITGAFLLGDNL
                     AERLYFISLTGMGMLTTVGVIVSMDTYGPVSDNAQGIAEMSGEFEGRPAEILQSLDAV
                     GNSTKAITKGMAIATAVIAATSLFGSFREILEGAGLEFEGIRIDEPQVLVGLLIGGSV
                     SFLFAALAIRAVGRAAAKVVVEVRNQFRDHPGIMEGTETPDYGRVVDICTRTSLRELL
                     TPGLLAVLTPVVVGFALEAEALGAYLAGVILTGQLLAVMLSNSGGAWDNAKKLVEEGH
                     HGGKGSEPHKATVIGDTVGDPFKDTAGPALNPLIKVMNLVALLIAPIVVAAADDIGTR
                     IVVSVLGTLILGLAIWHSKRLRADEILGAAEPPSPEAAEVTTQAS"
BASE COUNT          763 a         1575 c         1667 g          624 t
ORIGIN      
        1 gtgaacaccc cgaccaggta gacggcgatc agccgcgtga gctccgcgtt gaacagccac
       61 acgaggagcg ccgaggagac ggccaggacc aggatcccgt tcgagaacac gaggcggtcg
      121 ccgcggttgc ggaactgtcc aggcatgaac cggtccctcg caaggatcgc ggagagacga
      181 gggaagtcct ggtacgcggt gttcgccgcg gcgatcagga tcatcgccgt gaacgcctgc
      241 aggacgaaga acatgaccga ccgaccgaag atcgtctcgc cgatctgcgc caggaccgac
      301 cgggcccccg cgatctcctc ggtcacgcgc acctcgagca gggaggccat gagggtgacg
      361 cccaggaaca tcgtgaccga gatcgcggcc atcatcgcga gggtggcggc ggcgttccgg
      421 gcgcgcgggc ggcggaaggc ctggacgccg tcggcgaccg cctcgacacc tgtcagcgcc
      481 gaggacccgg acgtgaacgc gaggaggatc aggaagaggt tcagcgtgct ctcctcgtgc
      541 agcggcaggt ccgccgtctc ggcccggggg cagacgtcca tgcaccggat gagccccgcg
      601 ccgaccgtga cgtagatcga ggcgacgaac ccgtacgtcg ggacggcgaa gagcatcccc
      661 gactcgcgga cgccgcggag gttcgcgagc gagatcaacg cgatgaagcc catcgccaac
      721 gtgatcttgt gctcggccag ggccggcacg gcggaggtca ccgcgaccgc gccggccgtc
      781 gcactgacgg aggcggtgac gacgtagccg atcaggatcg ccccggccgc caggagcccg
      841 gggatcgtcc cgaggttctc gcgcgccacg atgtaggacc cgccgccttg cgggtacgcg
      901 cggaccgtct gccggtagga ggtcaccacg acgaggagga ccagggcgat cgcgccgccg
      961 atcggcagca tgagggtgag ggccgcggcg cccccgacca ccagggcgag catcatctcc
     1021 tcggtggcgt aggcgaccga tgacagcggg tcggaagcga acaccggcag cgcgagcgtc
     1081 ttcggcagga gatggtgctc ggccttctcc gaggcgatcg ctcggcccag gatgaggcgc
     1141 ttcagccgcc cggcgacggt agccacgggg gggattctat gggtggcggg cgcttccgcc
     1201 gaagtgcgcc ccggccctcg ggcgcgctag gctctccccg tgcacgtcat catcatgggg
     1261 tgcggccgcg tgggagccga gctcacgctc cagctctcga aggccggcca ctcggtcgcg
     1321 gtcatcgaca agcgggcgag cgccttcgac cgcctcccgc cgggcttcca ggccaaccgg
     1381 gtggtgggct acggcttcga ccgagaggtc ctggcgaagg ccggcatcga ccaggcggac
     1441 gcgttcatcg cggtctcgaa cggcgacaac tcgaacatcc tgtcggcgcg cgtggcgcgg
     1501 gagcactaca aggtgccgaa ggtgatcgcg cggatctacg acccgcgccg ggccgagatc
     1561 tacgagcggc tgaacatccc gaccgtcgcc accgtcatct gggccgcgaa gcagatccag
     1621 tacctcctct tccacggacg tgaggagctg aaggagacgt tcgccggcgg gacgctgatg
     1681 cgactccagg tgaaggtgcc atcccacctc gtcggcaagc cgatcgactc ggtcgtggag
     1741 acggggaagg tgacggtgat cggcgtcgac cgaggcggcg gtgggttcat cccgacgggt
     1801 gggtcgacgt tccaggaagg cgacgtcgcg cacttcatcg ttcacaagga cgcgatggac
     1861 cgctacgact tcctgatgga gcccgaggcg gagtgacgtg aggatcgtcg tcacgggcgc
     1921 agggaagatc ggccgccacc tggcgaacga cctccaggag cgggggcacg aggtcacgct
     1981 cgtcgagcag gactcgggcg agatggagcg ggcccgcggc gaggcggtcg aagggctgac
     2041 gctcgtgctc ggcgacgcct gcgagccgtg ggtgctcgat gcagccgagc tgcggacgtg
     2101 cgacgtcgtc gtggccgcga ccggggacga cgaggacaac ctcgtcacgt cgctgctcgc
     2161 gaagcaggag tacggggtcc cgcgcgtgat cgcgcgcgtg aaccacccca acaacgagtg
     2221 gctgttcacc gagcagtggg gcgtcgacgt cgcggtgagc ccgccgcacg tgctgacggc
     2281 gctcgtcgag gaggcggtga cgaccggcga cctggtccgc ctgctgaagc tcgagggcgg
     2341 ggaggtctcg ctcgtcgagg tgaagctgac ggagtcgtca cacatggtcg ggcgcccgct
     2401 ctacgagctg cgcctgcctc aggatgccgc ggtggtcgcg gtcctgcgcg gcggccacgt
     2461 cgtgatcccc cagcccgaaa cccagctcgc cgccggcgac gaggtcatcg cgatcgccac
     2521 ccccgagaac gaggaccaga tccgcaccgc cctcatgggc tagcgaacga cccgcaagcg
     2581 gtgggtcgcc gtcggtcagt gggagccgac gaactcgtag ccggggttga cgacccgcag
     2641 gcggcccttc tcctcgacga ccatgccctc gagcaggacc cgggtgccga ggccgacgcc
     2701 ggggatgtgg ctgcggccgg tccagaccgc gacgacctcg ccggtgccgt cggtgacgag
     2761 cgcctcgagt gactcctcgc cggggcggat cgtcatgcgg cgcacgacgc cggcgatccg
     2821 gcagcgggag cggcgttcgc acgagccgat cggcgtcgcg ccggcgacct cggccgccca
     2881 ctgccgcgtc tcctcgcgca agcgctcctc gtcggactgc cccatgcgct cccgcatgcg
     2941 gcggaaccag ctcatacccc ggagcctagc tcgcctgggt cgtgacctcg gccgcctccg
     3001 ggctcggggg ctcggcggca ccgaggatct cgtcggcgcg caggcgcttc gagtgccaga
     3061 tcgcgaggcc gaggatcagc gtgccgagga ccgagacgac gatccgcgtc ccgatgtcgt
     3121 cagccgcggc caccacgatc ggggcgatca ggagcgccac gaggttcatg accttgatca
     3181 gcgggttcag cgccgggccc gcggtgtcct tgaacgggtc gcccacggtg tcgccaatca
     3241 cggtcgcctt gtgcggctcg gatcccttgc cgccgtggtg cccctcctcg acgagcttct
     3301 tcgcgttgtc ccacgcgccg cccgagttcg acagcatgac ggccaggagc tgtcccgtga
     3361 ggatcacgcc ggcgaggtag gcgccgagcg cctcggcctc gagggcgaac ccgacgacga
     3421 ccggggtgag gaccgcgagg agccccggcg tgagcagctc ccgcagcgag gtccgcgtgc
     3481 agatgtcgac cacgcggccg tagtcgggcg tctcggtccc ctccatgatc cctgggtggt
     3541 cgcggaactg gttccgcacc tcgacgacaa ccttcgccgc ggcacgcccg accgcgcgga
     3601 tcgcgagcgc ggcgaagagg aacgagacgg atccaccgat cagcaggccg acgagcacct
     3661 gcggctcatc gatgcggatc ccctcgaact cgagcccggc tccctcgagg atctcgcgga
     3721 acgacccgaa cagcgacgtg gccgcgatga cggccgtcgc gatcgccatc cccttcgtga
     3781 tcgccttcgt cgagttcccg acggcgtcga ggctctggag gatctcggcc ggtcgcccct
     3841 cgaactcgcc cgacatctcc gcgatgccct gtgcgttgtc cgagaccggg ccgtacgtgt
     3901 ccatcgagac gatgacgccg acggtcgtga gcatccccat cccggtgagc gagatgaagt
     3961 agaggcgctc cgcgaggttg tccccgagca ggaacgcccc ggtgatcgcg ccgccgatca
     4021 cgaggatcgc ccacacggtg gactcgagcc cgaccgagaa cccggagagg atcgtcgtcg
     4081 cgggtcccgt cagcgatgac tccgcgacct ccttcacggg gcgacgcgtg gtcgaagtga
     4141 agtgctccgt caggagctgg atcacgctcg cgagcaggag gccgatcagc acggcccaga
     4201 agccgcggat gtcgtcgagg tagccggtcg cgacgaagta gaccgcggct gccgacagga
     4261 ccgcggagaa gaagaacccg cggttgatcg agcgcatgcc gtgctcctcc tcggagcgcg
     4321 gcgtcacgga gtagatcccg atgatcgagg tgatgacacc gatcgcgcgg acgaacagcg
     4381 ggaacatcac gccgacgatc gcgccctgcg gcgagccggc gaacgcggcg gcgccgagga
     4441 tgatcgaggc gacgagcgtc acctcgtacg actcgaagag gtcggcggcc atcccggcgc
     4501 agtcaccgac gttgtcgccg acgttgtcgg cgatgaccgc cgcgttgcgc gggtcgtcct
     4561 cggggatgtt cttctcgacc ttgccgacga ggtcggcgcc gacgtcggcg ccgacctcgt
     4621 cggcaaggt
//
LOCUS       DATLGG010000071         9510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_37363, whole genome shotgun sequence.
ACCESSION   DATLGG010000071 DATLGG010000000
VERSION     DATLGG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37363"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /gene="rplJ"
                     /locus_tag="VM638_01850"
     CDS             <1..766
                     /gene="rplJ"
                     /locus_tag="VM638_01850"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HVM11207.1"
                     /translation="VQELTERLREVDAAVFADFRGLSVSDAGELRTALAEVDTQLAVV
                     KNTLARIAARDAELDGVEGFLEGPTAIAFVKGDPVAAAKAIVDAAKRFPVLEVKGGYA
                     EGRVLSAADVGSLAALDSREVMLARLAGLFQAQMSRTAYLLQALQSKLLSLLEAYREK
                     LPSDGGTDPGAEDQPAAAEAEQAADAEGEQPADAETDAGPAAETEQPADAEAAETEPP
                     AETETETDPDATPGAETTETGTTETEPQGEGEQEGS"
     gene            767..1171
                     /gene="rplL"
                     /locus_tag="VM638_01855"
     CDS             767..1171
                     /gene="rplL"
                     /locus_tag="VM638_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HVM11208.1"
                     /translation="MATKKMSADDLLDAFKEMTLLELSEFIKQFEETFDVQAAAPMAG
                     MVMAPGAGAGGDGAGAEEEEQTEFDVILTGAGDKKIQVIKEVRGLTNLGLKEAKDLVE
                     AAPKPVLERVPKEQAEQAKEKLESAGGTVEIK"
     gene            1287..4721
                     /locus_tag="VM638_01860"
     CDS             1287..4721
                     /locus_tag="VM638_01860"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVM11209.1"
                     /translation="MTADHARVSFGKIEEVLPLPDLVGIQRYSFEWLKDEGLGEIFRE
                     ISPIEDYTETYQLMFGKHHFKDIKHTEEECRDKDMTFSAPLFVEAAFINKDTGEIKEQ
                     EVFMGDFPMMTEKGTFIINGTERVVVSQLVRSPGVYFDRQLDKTVDKDIYIAKIIPAR
                     GAWLEFDVDKKDTVGVRIDRKRRQHITVLLKALGWSEDEILELFDGAETIKHTLEKDT
                     LTTAEEALEDIYRKLRPGEPPTAESARNLLESLFFGTKRYDLAKVGRYKVSKKLEPAD
                     EILTRQLKARFQEMKELDNPDKRDWEQPRYRVFAEPQTGEKGGPEHKPILTYEDIAKT
                     VRYLVKLHAGEDGYEVDDIDHFGNRRIRSVGELIQNQIRIGLSRMERVVRERMTTHEV
                     EAITPQTLINIRPVVGSIKEFFGTSQLSQFMDQTNPLAGLTHKRRLSALGPGGLSRER
                     AGFEVRDVHPSHYGRMCPIETPEGPNIGLMGSLSSYGRINPFGFIETPYRRVNKGRVT
                     DQIDYLAADEEDRHVIAQANAVLDARGRFAEERVLVRRKGGEVDSVPPEEVDYMDVSP
                     KQLVSVAASLIPFLEHDDANRALMGANMQRQAVPLLRSEAPLIGTGVERRAAIDAGDV
                     VLAEKAGVVEDVSAEEIVIRETNGNLRPYRLLKFERSNQGTCINQRPVVKAGQRVKAG
                     DLIADGPSTEEGEIALGKNLLVAFMPWEGHNFEDAIVISERLVKEDILSSIHIEEHEV
                     DARDTKLGAEEITRDIPNVSEEILKDLDERGIVRIGAEVNPGDYLVGKVTPKGETELT
                     PEERLLRAIFGEKAREVRDTSLKVPHGESGKVIGVRVFSREEGDELAPGVNQLVRVYV
                     AQKRKISDGDKLAGRHGNKGVIAKILPEEDMPFLPDGTPVDIVLNPLGVPSRMNLGQV
                     LEAHLGWAAHQGFSKDGKLIEGPVKVATPVFDGAREGEIHEALASAAPNADGMKLIDE
                     HGKARLFDGRSGEPFEEPVAIGYIYIMKLLHLVDDKIHARSTGPYSMITQQPLGGKAQ
                     FGGQRFGEMEVWALEAYGAAYALQELLTIKSDDVLGRVKVYEAIVKGENIPEPGIPES
                     FKVLIKEMQSLALNVEVLSAEGKEIEMRDTDEDVFRAAEELGIDLSRREPAGAEFD"
     gene            4775..8677
                     /locus_tag="VM638_01865"
     CDS             4775..8677
                     /locus_tag="VM638_01865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HVM11210.1"
                     /translation="MLDVNYFDELRIGLATDDQIRAWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTRDWECHCGKYKRVRFKGIICERCGVEITRSKVRRDRMGHIDLAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYIITRVDEERRHKDLPKIEKEFASERA
                     EIESRRDEQIEARLRELEKRLKALEKEGAKKAQLTAAHKEAQREVDKITEAARRETDH
                     LDDVLNAFKNLSVKQLVADDIVYRTLRDRFEDYFRGGMGAEAIRDLLEDLDLEAEADD
                     LREQIKTAKGTRRQKAIKRLKVVSNFIGTKNSPLGMVLKAVPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLIDLQAPEIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKLHQCGLPKQMALE
                     LFKPFVMKRLVDQSLAQNIKSAKRMVERARPQVWDVLEEVIKDHPVLLNRAPTLHRLG
                     IQAFEPVLVEGKAIQIHPLVCTAFNADFDGDQMAVHVPLSSEAQAEARVLMLSANNIL
                     SPAHGRPIATPTQDMVLGVYYLTFAGDEPGKVEELPRFSGVAEVELALDARAVRLHDW
                     IRVRLPGKTLSPADLPEGGENGHAPLITTTVGRVVFNEAFPPDFPFVNRHVMKGDLAA
                     LVDRCVRTFPRSKVASVLDALKQLGFHYATRAGVTVGVDDVTVPPTKQKILDNYEKQA
                     LKVESNYRKGVITADERRQELIEIWTQATDEVKDAMEAEFQPTNPIFMMANSGARGNI
                     MQIRQIAGMRGLVANPKGEIIPRPIKANFREGLSVLEYFISTHGARKGLADTALRTAD
                     SGYLTRRLVDVAQEVNVREEDCGTDKGMAIRITVEAPDGSRVAAPYLDTSAFGRVLAE
                     DVRVNRSKIASAGDELSDELRDKLVEAGVEEVFARSVLTCDSVVGVCRLCYGRSLATG
                     RLVDIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGEDITHGLPRVVELFEARKPKGE
                     AQITELSGRVTIEDDDEKKVRKITVTADDGEQVQYAVSLRARLAVREGDEVSVGQQLT
                     EGSVNPHEKLRVEGVQAVQRHLAEEVQEVYRSQGVTIHDKHIELIIRQMLRKVHILEP
                     GDTDFLPGELVDRRRFEERNAEVVEGGGEPASARPMLMGITKASLATDSWLAAASFQE
                     TTRVLTDAAISAKSDPLLGLKENVIIGKLIPAGTGMPQYRNVRAHIRPEAIPEYWLVR
                     QQEAADAGFGGADGDGELTAEEARQLLAVDGEAEQGEG"
     gene            8786..>9510
                     /locus_tag="VM638_01870"
     CDS             8786..>9510
                     /locus_tag="VM638_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11211.1"
                     /translation="MRRPLIAVLALFTLGLPGGVTAQEGPQVLTSDNVEFVGTIPVAA
                     AASSIQFASDAPIAYISTFRGLEVWDITNPALPLPLGRAALGGWQNEAMSLGERPNGD
                     RFVLFAQNTAVLTQADAVYGRRVTVVEVTDPRNPVVHGTVTTPTRTHTASCVNKECTF
                     AYTDGRTQGRISVIDLRDFRNPKMASIPELTTPVAQGHDQDVDEAGILWHVGGQGSVA
                     MDVTDPERPRLLNSTTQAGATPP"
BASE COUNT         1714 a         3176 c         3317 g         1303 t
ORIGIN      
        1 ggtccaggag ctgacggagc ggctgcggga ggtcgacgcc gccgtcttcg cggacttccg
       61 tgggctctcg gtgtccgacg cgggtgagct ccgcacggct ctcgccgagg tcgacaccca
      121 gctcgccgtc gtgaagaaca ccctggcgcg gatcgccgcg cgcgatgcgg agctcgacgg
      181 cgtcgagggt ttcctcgagg ggccgaccgc catcgcgttc gtgaaggggg atcccgtcgc
      241 ggccgcgaag gcgatcgtcg acgccgcgaa gcgcttcccc gtcctcgagg tgaagggcgg
      301 ctatgccgag ggccgcgtcc tcagcgccgc cgacgtgggc tcgctcgccg cgctcgactc
      361 gcgagaggtg atgctcgctc gcctcgccgg cctcttccag gcccagatgt cgcggacggc
      421 gtacctgctg caggcgctcc agtcgaagct gctcagcctc ctggaggcct accgcgagaa
      481 gctgccgtcc gacgggggca ccgaccccgg agccgaggat cagcccgccg cagccgaggc
      541 cgagcaggcc gcggatgccg agggcgagca gcctgcggac gccgagaccg acgccggccc
      601 cgcggccgag accgagcagc cggccgacgc cgaggccgcc gagacggagc cgcccgcgga
      661 gaccgagacc gagacggacc cggacgcgac gcccggggcc gagaccaccg aaaccggaac
      721 gaccgagacc gagcctcagg gcgaagggga acaggagggt tcctagatgg cgaccaagaa
      781 gatgtcggcc gacgacctgc tcgacgcgtt caaggagatg acgctgctcg agctgtcgga
      841 gttcatcaag cagttcgagg agaccttcga cgtccaggcc gccgcaccga tggccgggat
      901 ggtcatggct cccggcgcgg gcgccggcgg cgacggcgcg ggtgccgagg aggaggagca
      961 gaccgagttc gacgtcatcc tcaccggcgc cggcgacaag aagatccagg tcatcaagga
     1021 ggtccgtggc ctcacgaacc tcggcctcaa ggaggcgaag gacctggtcg aggcggcgcc
     1081 gaagcccgtc ctggagcggg tgccgaagga gcaggccgag caggcgaagg agaagctgga
     1141 gtcggcgggc ggcaccgtcg agatcaagta gccatctcca cttgtcgctc ggggcccctg
     1201 ggcctatact gtccgtttgc cgtgccgtct tccgcgtgtg gtagttccac gcatgtcatt
     1261 ccctgggggg gatatctgtg cctgaggtga ccgccgacca cgcccgcgta tcgttcggga
     1321 agatcgagga ggttcttccg ctcccggatc tcgtgggtat ccagcggtat tcgttcgaat
     1381 ggctgaagga cgagggtctc ggcgagatct tccgcgagat cagcccgatc gaggattaca
     1441 ccgagacgta ccagctgatg ttcgggaagc accacttcaa ggacatcaag cacaccgagg
     1501 aggagtgccg ggacaaggac atgaccttct cggcgccgct gttcgtcgag gccgccttca
     1561 tcaacaagga caccggcgag atcaaggagc aggaggtctt catgggcgac ttccccatga
     1621 tgaccgagaa gggcaccttc atcatcaacg gcaccgagcg cgtcgtggtc tcccagctgg
     1681 tccgctcgcc gggcgtctac ttcgaccgtc agctcgacaa gacggtggac aaggacatct
     1741 acatcgcgaa gatcatcccc gcccgcggcg cctggctcga gttcgacgtc gacaagaagg
     1801 acacggtcgg cgtccggatc gaccgcaagc gccgccagca catcaccgtc ctgctcaagg
     1861 cgctcgggtg gagcgaggac gagatcctcg agctgttcga cggcgccgag acgatcaagc
     1921 acaccctcga gaaggacacc ctgaccacgg ccgaggaggc cctcgaggac atctaccgca
     1981 agctccgccc gggtgagccg ccgaccgccg agtcggcgcg gaacctgctc gagagcctgt
     2041 tcttcgggac gaagcgctac gacctcgcga aggtcggccg ctacaaggtc tccaagaagc
     2101 tcgagccggc cgacgagatc ctcacccgtc agctcaaggc gcggttccag gagatgaagg
     2161 agctcgacaa ccccgataag cgggattggg agcagcctcg ctaccgggtc ttcgccgagc
     2221 cgcagacggg cgagaagggc gggcccgagc acaagccgat cctcacctac gaggacatcg
     2281 cgaagaccgt ccgctacctc gtgaagctgc acgccggcga ggacgggtac gaggtcgacg
     2341 acatcgacca cttcggcaac cggcgcatcc gctcggtcgg cgagctcatc cagaaccaga
     2401 tccggatcgg tctgtcgcgg atggagcgtg tcgtccgcga gcgcatgacc acccacgagg
     2461 tcgaggcgat caccccgcag accctgatca acatccgtcc ggtcgtcggg tcgatcaagg
     2521 agttcttcgg cacctcgcag ctctcccagt tcatggacca gacgaacccg ctcgccggcc
     2581 tgacgcacaa gcggcgtctg tcggcgctcg ggccgggcgg gctctcgcgc gagcgcgccg
     2641 ggttcgaggt ccgcgacgtc cacccgtccc actacgggcg catgtgcccg atcgagacgc
     2701 cggaggggcc gaacatcggc ctcatggggt cgctgtcgag ctacgggcgc atcaacccgt
     2761 tcgggttcat cgagacgccc taccggcgcg tgaacaaagg acgggtgacc gatcagatcg
     2821 actacctcgc ggccgacgag gaggaccggc acgtcatcgc acaggcgaac gccgtgctcg
     2881 acgcgcgtgg gcgcttcgcc gaggagcgcg tcctcgttcg ccgcaagggc ggcgaggtcg
     2941 actcagtgcc gccggaagag gtcgactaca tggacgtctc gccgaagcag ctggtctcgg
     3001 tggcggcgtc gctgatcccc ttcctcgagc acgacgacgc caaccgggcc ctgatgggcg
     3061 cgaacatgca gcgtcaggcg gtgccgcttc tccgctcgga ggcgccgctg atcggtaccg
     3121 gcgtcgagcg acgcgcggcg atcgatgccg gcgacgtcgt gctggccgag aaggccggcg
     3181 tcgtggagga cgtctccgcc gaggagatcg tgatccgcga gacgaatggc aacctgcgcc
     3241 cgtaccggct gctcaagttc gagcgctcga atcaggggac ctgcatcaac cagcgtcccg
     3301 tggtgaaggc cggacagcgg gtgaaggccg gtgacctgat cgccgacgga ccgagcaccg
     3361 aggagggcga gatcgcgctc ggcaagaacc tcctcgtcgc gttcatgccg tgggagggcc
     3421 acaacttcga ggacgcgatc gtcatctcgg agcgcctcgt gaaggaggac atcctctcct
     3481 cgatccatat cgaggagcac gaggtcgacg cccgcgacac gaagctcgga gccgaggaga
     3541 tcacgcggga catcccgaac gtctcggagg agatcctcaa ggacctcgat gagcgcggga
     3601 tcgtccggat cggcgccgag gtcaacccgg gtgactacct cgtcgggaag gtcacgccga
     3661 agggagagac cgagctgacg ccagaggagc gcctcttgcg cgcgatcttc ggggagaagg
     3721 cacgcgaggt gcgcgacacc tcgctcaagg tcccgcacgg cgagtccggg aaggtcatcg
     3781 gggtgcgggt cttctcgcgt gaggagggcg acgagctcgc gccgggggtg aaccagctcg
     3841 tgcgcgtcta cgtcgcgcag aagcggaaga tctccgacgg cgacaagctc gccgggcgcc
     3901 acgggaacaa gggcgtcatc gcgaagatcc tccccgagga ggacatgccg ttcctgcccg
     3961 acgggacgcc cgtggacatc gtcctgaacc cgctcggcgt cccgtcccgg atgaacctcg
     4021 ggcaggtgct cgaggcgcat ctcggctggg cggcccacca gggcttctcg aaggacggga
     4081 agctgatcga gggtcccgtg aaggtcgcga ccccggtctt cgacggcgcc cgcgaaggcg
     4141 agatccacga ggcgctcgcc agcgcggctc cgaacgccga cggcatgaag ctgatcgacg
     4201 agcacgggaa ggcgaggctg ttcgacggtc gctccgggga gccgttcgag gagccggtgg
     4261 cgatcggcta catctacatc atgaagctgc tccacctggt ggacgacaag atccacgcca
     4321 ggtccaccgg cccctactcg atgatcaccc agcagccgct gggcgggaag gcgcagttcg
     4381 gcggtcagcg cttcggcgag atggaggtct gggcgctcga ggcctacggc gccgcgtacg
     4441 ccctgcagga gctgctgacg atcaagtccg acgacgtcct cggccgtgtc aaggtctacg
     4501 aggcgatcgt caagggcgag aacatccccg agccgggcat cccggagtcg ttcaaggtcc
     4561 tcatcaagga gatgcagtcc ctcgccctca acgtcgaggt cctctcggcg gagggcaagg
     4621 agatcgagat gagggatacc gacgaggacg tcttccgggc ggccgaggag ctcgggatcg
     4681 acctctcgcg gcgggagccg gcgggagccg agttcgacta gtgacgaggc gtgtgcgggt
     4741 tggcgagcgc ggagctgagg aggcgagcgt ctaagtgctg gatgtgaact acttcgatga
     4801 gctgaggatc ggcctggcga ccgatgacca gatccgggcc tggtcgaacg gcgaggtcaa
     4861 gaagcccgag acgatcaact accggaccct gaagccggag aaggacggac tcttctgcga
     4921 gaagatcttc ggcccgaccc gggactggga gtgccactgc ggcaagtaca agcgcgtccg
     4981 gttcaagggg atcatctgcg agcgctgcgg cgtcgagatc acgcgttcca aggtccgtcg
     5041 cgaccgcatg ggccacatcg acctcgcggc cccggtgacg cacatctggt acttcaaggg
     5101 cgtcccctcg cgcctcggct acctcctcga cctcgccccg aaggacctcg agaaggtcat
     5161 ctacttcgcg gcgtacatca tcacgcgggt cgacgaggag cgacgccaca aggacctgcc
     5221 gaagatcgag aaggagttcg cctccgagcg ggccgagatc gagtcccgtc gggacgagca
     5281 gatcgaggcg cgcctgcgcg agctcgagaa gcggctcaag gctctcgaga aggagggcgc
     5341 caagaaggcc cagctcacgg ccgcccacaa ggaggcccag cgggaggtcg acaagatcac
     5401 cgaggcggcc cgccgcgaga ccgaccacct cgacgacgtg ctgaacgcct tcaagaacct
     5461 ctccgtgaag cagctcgtcg ccgacgacat cgtctaccgg accctgcgcg accgcttcga
     5521 ggactacttc cgcggcggga tgggggccga ggcgatccgc gacctgctcg aggacctcga
     5581 cctcgaggct gaggccgacg acctccgcga gcagatcaag acggcgaagg ggacccggcg
     5641 tcagaaggcc atcaagcggc tcaaggtcgt ctcgaacttc atcgggacga agaactcgcc
     5701 gctcggcatg gtcctgaagg cggttccggt gatcccgccg gacctgcggc cgatggtcca
     5761 gctcgacggt ggccggttcg cgacctccga cctcaacgac ctgtaccggc gcgtgatcaa
     5821 ccggaacaac cgtctgaagc ggctgatcga cctccaggcg ccggagatca tcgtcaacaa
     5881 cgagaagcgg atgctccagg aggccgtcga cgccctcttc gacaacgggc gccgcgggcg
     5941 tccggtcacc gggccgggca accgtccgct gaagtcgctg tcggacatgc tgaagggcaa
     6001 gcagggacgc ttccgccaga acctgctcgg caagcgcgtg gactactccg ggcgctcggt
     6061 gatcgtcgtc gggccgaacc tcaagctcca ccagtgcggc ctgccgaagc agatggcgct
     6121 cgagctgttc aagcccttcg tgatgaagcg gctcgtcgac cagagcctcg cgcagaacat
     6181 caagagcgcc aagcgcatgg tcgagcgcgc ccggccgcag gtctgggacg tgctggagga
     6241 ggtcatcaag gaccacccgg tgctcctgaa ccgcgcgccg accctgcacc gcctcgggat
     6301 ccaggcgttc gagccggtct tggtcgaggg gaaggcgatc cagatccacc ccctcgtctg
     6361 caccgcgttc aacgccgact tcgacggcga ccagatggcc gtccacgtgc cgctgtcctc
     6421 ggaggctcag gccgaggcgc gcgtgctcat gctgtcggcg aacaacatcc tgtcgccggc
     6481 gcacggccgt ccgatcgcca cgcccaccca ggacatggtg ctcggcgttt actacctcac
     6541 gttcgcgggc gacgagcctg ggaaggtcga ggaactgccg cggttctcgg gtgtggccga
     6601 ggtggagctg gcgctcgacg cccgcgcggt ccggctccac gactggatcc gggtccggct
     6661 tccgggcaag acgctctcgc cggccgacct ccccgagggg ggcgagaacg gccatgcgcc
     6721 gctcatcacg acgacggtcg ggcgggtcgt cttcaacgag gccttcccgc cggacttccc
     6781 cttcgtgaac cgccatgtga tgaagggtga tctggcggcc ctcgtggacc gctgcgtccg
     6841 gacgttccct cggagcaagg tggcgtcggt cctggacgcg ctgaagcagc tcgggttcca
     6901 ctacgcgacc cgggccgggg tcaccgtcgg cgtcgacgac gtcaccgtgc ccccgacgaa
     6961 gcagaagatc ctcgacaact acgagaagca ggcgctcaag gtcgagtcga actaccgcaa
     7021 gggcgtcatc accgccgacg agcggcgtca ggagctcatc gagatctgga cgcaggcgac
     7081 cgacgaggtg aaggacgcga tggaggcgga gttccagccg acgaacccca tcttcatgat
     7141 ggcgaactcg ggagcccgcg ggaacatcat gcagatccgg cagatcgccg ggatgcgtgg
     7201 tctcgtggcc aacccgaagg gcgagatcat cccgcggccg atcaaggcga acttccgcga
     7261 gggcctgtcc gtcctcgagt acttcatctc gacccacgga gcccggaagg gtctcgccga
     7321 caccgccctc cgcaccgccg actcgggcta cctcacgcgg cgtctcgtcg acgtcgccca
     7381 ggaggtgaac gtccgcgagg aggactgcgg gaccgacaag gggatggcga tccggatcac
     7441 cgtcgaggcg ccggacggca gccgcgtggc cgctccgtac ctcgacacga gcgccttcgg
     7501 ccgggtcctc gccgaggacg tccgggtcaa ccgctcgaag atcgcctcag ccggcgacga
     7561 gctcagcgac gagctccggg acaagctggt cgaggcgggc gtcgaggagg tcttcgcccg
     7621 ctcggtcctc acctgcgact ccgtcgtcgg ggtgtgccgc ctctgctacg ggcggagcct
     7681 cgcgacgggc cggctcgtcg acatcggcga ggccgtcggc atcatcgcgg cccagtcgat
     7741 cggtgagccg gggacccagc tcacgatgcg gaccttccac accggtgggg tcgccgggga
     7801 agacatcacg cacggcctcc cgcgcgtcgt cgagctcttc gaggcgcgga agccgaaggg
     7861 cgaggcgcag atcaccgagc tctcgggccg cgtcacgatc gaggacgacg acgagaagaa
     7921 ggtccgcaag atcacggtga cggcggatga tggggagcag gtccagtacg cggtctcgct
     7981 ccgcgcgcgc ctggccgtcc gggagggcga cgaggtctcc gtcggccagc agctcaccga
     8041 gggttcggtg aacccgcacg agaagctgcg cgtcgagggt gtccaggcgg tccagcgcca
     8101 cctggccgag gaggtccagg aggtctaccg gagccagggc gtcacgatcc acgacaagca
     8161 catcgagctg atcatccggc agatgctgcg gaaggtgcac atcctggagc ccggcgacac
     8221 cgacttcctg ccgggagagc tggtggaccg gaggcggttc gaggagcgca acgccgaggt
     8281 cgtggagggc ggcggcgagc cggcgtcggc gcggccgatg ctcatgggca tcacgaaggc
     8341 ctcgctcgcg accgactcgt ggctggcggc ggcttcgttc caggagacga cgcgggtact
     8401 caccgacgcg gcgatcagtg cgaaatccga cccgctcctc ggcctgaagg agaacgtcat
     8461 catcgggaag ttgatcccgg ccgggaccgg catgccgcag taccgcaacg tccgggcgca
     8521 catccggccg gaggccatcc ccgagtactg gctggtccgt cagcaggagg cagcggacgc
     8581 aggcttcggc ggcgccgacg gtgacgggga gctcacggcc gaggaggcga ggcagctcct
     8641 cgcggtcgac ggcgaggccg agcagggcga gggctaggca agaggttcca ccaccgggcg
     8701 gcggttcgac cgccgtccgg tggcaggagt tccgcgcgcg acgtcgaacc cactccgtaa
     8761 ccggaggaaa cggaaaggag acgtcgtgcg acgccccctg atcgctgtgc tcgccctctt
     8821 cacgctcggc ctgccaggcg gggtcaccgc ccaggaaggg ccgcaggtcc tgacgagcga
     8881 caacgtcgag ttcgtgggga cgatccccgt cgccgcggcc gcgtcctcga tccagttcgc
     8941 ctcggacgcg ccgatcgcct acatctcgac gttccgcggc ctcgaggtct gggacatcac
     9001 gaacccggcc cttccgctgc cgctcggccg cgccgccctc ggcggctggc agaacgaggc
     9061 gatgagcctc ggggagcggc cgaacggcga caggttcgtg ctgttcgccc agaacaccgc
     9121 agtcctcacg caggccgacg cggtctacgg gcgcagggtc accgtcgtcg aggtcacgga
     9181 cccgcggaac ccggtcgtgc acgggaccgt cacgaccccg acccggaccc acacggcgtc
     9241 ctgcgtcaac aaggagtgca ccttcgccta caccgacggg cggacgcagg gcaggatctc
     9301 cgtgatcgac ctccgcgact tccgcaaccc gaagatggcc tcgatccccg agctgacgac
     9361 gcccgtggcg cagggtcacg accaagacgt cgacgaggcg gggatcctgt ggcacgtggg
     9421 aggccagggc agcgtcgcga tggacgtgac cgaccccgag cggccgcggt tgctcaactc
     9481 gacgacgcag gcaggcgcca cgccgccgtg
//
LOCUS       DATLGG010000072         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_192487, whole genome shotgun sequence.
ACCESSION   DATLGG010000072 DATLGG010000000
VERSION     DATLGG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192487"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VM638_01875"
     CDS             <1..461
                     /locus_tag="VM638_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM11212.1"
                     /translation="WGSAAPGVSVRGEHFRVEDASIEPRPERQIPIWLGVYGRRALGV
                     AARLADGWIPSMPFASPERFVTLRDRLLTAAEEAGRDPGEITLAYNVGVQIQEGRVAD
                     ERSVAGGVAEVTDRLGDLIQRLGLGALSLWIRGEEQRQRFAEGILPALRR"
     gene            complement(464..1117)
                     /locus_tag="VM638_01880"
     CDS             complement(464..1117)
                     /locus_tag="VM638_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HVM11213.1"
                     /translation="MGERGSVSPIVAAALGLVGLFLAVVADLTYAAVARSRAQATADA
                     AALAAAQELVRPSGRSPSEVARRYAELGGARLLRCRCPSGGSDVEVEVRLEVDLPAIS
                     RSAAATASARAVVALPSGAAGLQPWFVARLRCLFSKVAGLTIVSGFRTYEEQARLHRE
                     RPDLAAPPGTSKHERGLAADLAFPSDEARSNAHRSAASCGLVFPVPHEDWHVEPVGA"
     gene            complement(1110..1487)
                     /locus_tag="VM638_01885"
     CDS             complement(1110..1487)
                     /locus_tag="VM638_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HVM11214.1"
                     /translation="MRRQRGSAAVELALVLPLLLTALLTLVQVAIVARDALVVAHAAR
                     EGAREAAVTLDEERIRAAVLRAGPHPDRTETAVERHGSVGDAVRVSVRYRVPLVVPFV
                     GWVFGEDATIAFASTMRQEVEGG"
     gene            complement(1484..1687)
                     /locus_tag="VM638_01890"
     CDS             complement(1484..1687)
                     /locus_tag="VM638_01890"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HVM11215.1"
                     /translation="MRGFLGRSIGGLRRRLDASSGQTTAEYALVILAAAAVAVALITW
                     ARSSGVLPSFFDQIIERLLGEGR"
     gene            complement(1687..2304)
                     /locus_tag="VM638_01895"
     CDS             complement(1687..2304)
                     /locus_tag="VM638_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVM11216.1"
                     /translation="MTTLLAAGATMAIGLLATAMCLRADGAVLRRMGPTARREPSSPG
                     RWDRGRWARRPRHGRDLASGVPDLIDLVAVAVSAGLTPRLALDRATSVVGGALGVELD
                     GCARSVALGRPWRIALRDLAERSGIADLRRLAVTLDRGERLGSPLAAQLRELARQARV
                     SQRIRQEDRARRAPVAMLFPLVFCILPAFVLAAVVPAVVSAARGL"
     gene            complement(2301..3125)
                     /locus_tag="VM638_01900"
     CDS             complement(2301..3125)
                     /locus_tag="VM638_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVM11217.1"
                     /translation="MIAWAYGGTAAAALLLAAAAAGHREREVVRRRVGASTEPRLRRA
                     VPERQVRLAAAFAGASALGWHLAGPVGAAAAPAGVGLVLRARRERRSVEERDRFDEQL
                     RDVAVGLAASTRAGLSLRRALREVTLEARPPLRPILERVVRRLEVGEPIADALAPLVQ
                     GSPDARLLTAVLEMHQRTGGDLPVMLEELAGTIGRRVEARHQARALTAQGRASGAVLA
                     VLPIAFVGLLSGTSGSALGAFYRTTLGSLLLLSGLALEAVGYLWLRRIVRGSEARA"
     gene            complement(3122..>3901)
                     /locus_tag="VM638_01905"
     CDS             complement(3122..>3901)
                     /locus_tag="VM638_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVM11218.1"
                     /translation="VDARLPDGSRVNAIIPPLSLKGPTLTIRKFSRDPYTMDDLITFG
                     SMTPQAAQFLAACVKGKLNVLISGGTGTGKTTMLNALSSFVPDDERIVTIEDAAELRV
                     ARPHVVRLEARPPNVEGRGAVTVRDLVRNALRMRPDRIIVGEVRGGEALDMLQAMNTG
                     HEGSMSTIHANAPDELASRLETMALMSDVDLPVAHIREQIAAAIDLIVQTVRQRDGRR
                     LVTRLAAVEGLRDGRIALRDVVVRDAAGARPPILSGMRGAG"
BASE COUNT          568 a         1463 c         1428 g          442 t
ORIGIN      
        1 gatggggatc tgccgctccg ggcgtgtcgg tgaggggcga gcacttccgg gtcgaggatg
       61 cgtcgatcga gccgcgcccg gagcggcaga tccccatctg gctgggggtc tacggccggc
      121 gcgcactcgg cgtcgcggca cggctggcgg acgggtggat cccctcgatg cccttcgcat
      181 cacccgagcg cttcgtgacg ctgcgcgacc gtctactgac cgcggccgag gaagcggggc
      241 gggacccggg agagatcacc ctcgcgtaca acgtcggcgt tcagatccag gaggggcggg
      301 tcgcggatga gcggtcggtg gcgggtggcg tggccgaggt caccgaccgc ctcggggatc
      361 tgatccagcg tctcgggctc ggggcgctca gcctgtggat ccgcggcgag gaacagcgtc
      421 agcgcttcgc cgaggggatc cttccggcgc tccgccggta ggctcaggcg cccaccggct
      481 cgacgtgcca atcctcgtgc ggcaccggga agacgaggcc gcacgaggcc gcggagcgat
      541 gcgcgttcga gcgtgcctcg tcggagggga aggcgaggtc ggccgcgagc ccgcgctcgt
      601 gcttcgacgt ccccggcggc gcggccaggt ccggacgctc gcggtgcagc cgcgcctgct
      661 cctcgtacgt gcggaacccc gagacgatcg tgaggcccgc gaccttcgag aagaggcagc
      721 ggagccgtgc cacgaaccac ggctgcaggc ccgccgcccc gctcggaagg gcgaccaccg
      781 cgcgtgccga cgccgtcgcg gcggcgctcc gcgagatggc cggcagatcc acctcgagcc
      841 gaacctcgac ctccacatcg gatccgcccg acggacagcg gcagcggagc agccgcgcgc
      901 cgccgagctc ggcgtagcgt cgagccacct cggaggggct ccggccggac ggccgcacga
      961 gctcctgggc ggcggcgagc gcggcggcgt cggcggtcgc ctgcgcgcgg gatcgagcga
     1021 ccgccgcgta ggtgaggtcg gcgaccacgg cgaggaacag accgacgaga ccgagggccg
     1081 ccgccacgat cggcgagacg ctcccacgct cacccaccct ccacctcctg tcgcatcgtg
     1141 ctggcgaacg cgatggtcgc gtcctcaccg aagacccacc cgacgaacgg cacgaccagg
     1201 gggacgcggt atcgaacgga gacccgcacc gcgtcgccga ccgacccgtg gcgttccacc
     1261 gcggtctccg tgcggtccgg atggggacct gcccggagca ccgccgcgcg gatccgctcc
     1321 tcgtcgaggg tgacggctgc ctcacgcgcg ccctcacgcg ccgcgtgcgc aacgacgagc
     1381 gcgtcccgcg ccacgatcgc gacctgcacg agcgtgagga gcgccgtcag gagcagcggc
     1441 agcacgagcg ccagctcgac cgcggcactg ccccgctgac gtctcaccga ccctcgccga
     1501 gcagtcgctc gatgatctgg tcgaagaacg acgggaggac gccgctcgac cgagcccagg
     1561 tgatcagcgc gacggcgacc gccgcggcgg cgaggatcac gagcgcgtac tccgcggtgg
     1621 tctgacccga cgacgcgtcg agcctccgcc gaaggccacc gatcgagcga ccgaggaacc
     1681 ccctcatcac agcccccgtg cggccgagac gaccgccgga accaccgccg cgaggacgaa
     1741 cgccggaagg atgcagaaga cgagcgggaa cagcatcgcg accggggcgc gccgcgcccg
     1801 gtcctcctgg cggatccgtt gggagacgcg ggcctggcga gcgagctcgc gcagctgcgc
     1861 cgccagcggc gagcccagac gctccccacg gtcgagcgtg acggccagcc gccggagatc
     1921 ggcgatgccg ctccgctcgg cgaggtcgcg cagcgcgatc cgccacggcc gccccagcgc
     1981 gacgctcctc gcgcacccgt cgagctcgac ccccagcgcg ccgccgacca cggaggtcgc
     2041 ccggtcgagc gccagacgcg gcgtcagccc ggcgctgacg gcgacggcga cgaggtcgat
     2101 caggtcggga acccctgagg cgaggtctcg gccgtgccgc ggccgccgag cccagcgccc
     2161 gcgatcccag cggccaggag atgagggctc gcgcctcgct gtcggcccca tccggcggag
     2221 cacggcgccg tcggcgcgca ggcacatcgc ggtcgcgagg agtccgatgg ccatcgtggc
     2281 gccggccgca aggagggtcg tcacgccctc gcctccgatc cgcggacgat acgacgcagc
     2341 cagagatagc cgaccgcttc gagcgcgagg ccgctcagga gcagcaacga cccgagggtg
     2401 gtccggtaga acgcgccgag ggcgctcccg ctcgtcccgg agaggagccc gacgaaggcg
     2461 ataggaagga cggcgagaac cgcccccgac gcgcgtccct gcgccgtgag cgcgcgcgcc
     2521 tgatggcgcg cctcgacacg gcgtccgatc gtgcccgcga gctcctcgag catcacggga
     2581 agatctccgc ccgtccgctg gtgcatctcg agcaccgccg tgaggagacg ggcgtctggc
     2641 gaaccctgca cgagcggcgc gagcgcatcg gcgatgggct cccccacctc gagccgtcgc
     2701 acgacccgct cgaggatcgg ccgcagcggg gggcgtgcct cgagggtgac ctcgcgtagc
     2761 gctcgacgca gcgacaggcc cgctcgcgtc gacgccgcga ggcccacggc aacatcacgg
     2821 agctgctcgt cgaaccgatc ccgctcctcg accgaccgcc gctcacgtct ggcccggagg
     2881 accaggccga cgcccgcggg cgcggccgcc gctccgacgg ggccggcgag gtgccagccg
     2941 agggccgacg cgccggcgaa cgcggcggcg aggcggacct gccgctcagg tacggcccgc
     3001 cggaggcgag gctcggtcga cgcccccacc ctcctgcgca cgacctcccg ctcccgatgc
     3061 cccgcggcgg cggcggcgag gagcagcgct gccgccgccg tcccgccgta ggcccaggcg
     3121 atcatccggc accccgcatc ccggacagga tcggtggccg cgctccggca gcgtcgcgca
     3181 cgaccacgtc gcggagcgcg atcctgccgt cgcggaggcc ttcgaccgcc gcgagtcggg
     3241 tgacgagccg acggccgtcg cgctggcgga ccgtctgcac gatgaggtcg atcgccgccg
     3301 cgatctgctc gcggatgtgg gccacgggca ggtccacatc cgacatcagc gccatcgtct
     3361 cgagcctcga tgccagctcg tccggcgcgt tcgcgtggat cgtcgacatc gacccctcgt
     3421 ggccggtgtt catcgcctgg agcatgtcga gggcctcgcc gccgcggacc tcgccgacga
     3481 tgatccggtc cgggcgcatc cgcagggcgt tccgcacgag gtcccggacc gtgacggcgc
     3541 cgcgtccctc gacgttggga ggccgggcct ccagacggac gacgtggggt cgcgcgacgc
     3601 gcagctccgc cgcgtcctcg atcgtcacga tccgctcgtc gtcggggacg aacgaggaga
     3661 gagcgttcag catcgtcgtc ttgccggtgc cggtgccgcc cgagatcagg acgttcagct
     3721 tccccttcac gcacgcggcc aggaactgcg ccgcctgcgg ggtcatcgac ccgaacgtga
     3781 tcaggtcgtc catcgtgtag gggtcgcgcg agaacttccg gatcgtcagc gtcggcccct
     3841 tgagcgagag cggcgggatg atcgcgttga cgcggctgcc gtccgggagc cgcgcgtcga
     3901 c
//
LOCUS       DATLGG010000073        12265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_37421, whole genome shotgun sequence.
ACCESSION   DATLGG010000073 DATLGG010000000
VERSION     DATLGG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37421"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VM638_01910"
     CDS             <1..1176
                     /locus_tag="VM638_01910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVM11219.1"
                     /translation="ALSVMTQDLPATADVVVAGGGVMGASTAYHLARMGAGRVVLVER
                     EGYLGEMSTGQCAGGIRHQFSTETNVRLSKESIAMMERFPEELGQPLDIDLCGYLILL
                     TDERDVAVFRENVALQRRLGVETEWLDPEQVHERIPLVNTDGVLAATYYDRDGLADPG
                     GVVQGYATAARGLGVTIATGCEVTGIETASGRIRAVETTRGRIETPVVVNACGAWAPH
                     LGAMVGVEIPIRPIRRQIVSTTPIPGLPPDFPFVVFFGTSLYLHREGEGILSGKSNND
                     EEPGFKLEVDEEWEAVHMMELMERFPAVEEAGIRARWAGLYEVTPDAHPIFGRVSPVE
                     GFYVMAGFSGHGFMHGPVMGLLMAEEILEGEARTVPVGEFRYERFLTGDLSPEYNVI"
     gene            complement(1173..2558)
                     /gene="cysS"
                     /locus_tag="VM638_01915"
     CDS             complement(1173..2558)
                     /gene="cysS"
                     /locus_tag="VM638_01915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVM11220.1"
                     /translation="MRLFNTLTRRVDDVRPVEEGHVKMYTCGPTVYRPVHIGNLRSFL
                     LSDLVRRGLELEGHRVTQVINITDVGHMVDDATDQDRMELAMEEQGLGPWEIAEKYTR
                     MFLEDTAKIGALPAHEYPRATDHIPEMLDMTRTLIDRGHAYELDDGTVYYDVRSFPAY
                     GALSRNTLENLEPGHRDLEAGEGKRHHADFALWKPAAPNRLMKWDSPWGEGFPGWHIE
                     CSAMSVKYLGERFDIHTGGTDLVFPHHEDEIAQSDAALGHQVISIWVHGGHLLAEGQK
                     MSKSKGNVYTIDDVVARGHDPLAFRYLCLQTRYRSVLDFRWEALDAADRKLTQIRQRL
                     AGWDGSSAELGSGAQDLDRRFREAVADDLDTPTAVAVLNETLSADVPDTDKRRLLEDW
                     DRFLGLDFGKLAREGFEIPGDVRSLMAERDAARAEKDYARSDEIRGRLTDMGWEVMDT
                     PEGTKVRPLLG"
     gene            2595..3734
                     /locus_tag="VM638_01920"
     CDS             2595..3734
                     /locus_tag="VM638_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM11221.1"
                     /translation="MDRDFGLDDEQREIVGLVREFVAERVAPQSAGYEERKEFPREAF
                     RALGEMGLGGIPYDESHGGGGQPFLTYLAVLEEIAAGHVALAVGLSVHHLSAFGVERY
                     GSDDLKQRYLPRLFSGEWLGAYALSEASSGSDAAALRTRAARTDAGWRLDGSKRFISH
                     AGEAEFYLVMARTGEDGPKGISAFAVEKDWPGFGFGKLEEKMGWHASPMSELLFDGCD
                     VPTENLVGEEGRGFEVALAALDSGRLGMAACAVGVAQAALEAAITFTKDRTQFGKPVI
                     EFQGLQFMLADMAVQVEAARRLYREAARARDAGQGTSVSCSMAKVFASDIAMRVTTDA
                     VQACGGYGYMQEYPLERYMREAKALQIVEGTNQVQRMIIGRHLAR"
     gene            3743..4201
                     /locus_tag="VM638_01925"
     CDS             3743..4201
                     /locus_tag="VM638_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11222.1"
                     /translation="MDQPPFDVIVSGRRHLLGAGAGAYEVWEKGREGETLASFPLTDA
                     GLRQAEAAFDELERRGRWSRPRVEAALRTALFWGLGAWLVIGLVEVIWGIVRDFAAIP
                     PEIGFSEPGTPWLRIVLQVLSVTAFRIWVAALVALAALRWLVPTGTEARP"
     gene            4198..5535
                     /locus_tag="VM638_01930"
     CDS             4198..5535
                     /locus_tag="VM638_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HVM11223.1"
                     /translation="MTVAERLTGVLPQVRQDLERLVRIPSVSAPGFDPEEVVRAATAT
                     AEVLEASGLGAVRILEMEGEHPAVYGETPAQDGAPTVLLYAHHDVQPPGRPEDWMSPP
                     FEPTERAGRLYGRGTADDKAGIAVHAAALRTHEGSPPVGVKVLIEGEEEIGSPNLPGF
                     LERYGEDLRADVIVLADMVNWRVGQPALTTSLRGLVDCYVEVRTLEHGIHSGMFGGAF
                     PDALSVLCRILATLHDDDGSVAVPGLSSGPADELDLTEAELRAQAGAVEGVALIGWGS
                     LTERVWTMPAVSVLAIDAPPVAEASNTLVPAARAKVSLRIAPGDSPEEAMRALLDHLR
                     SNAPWGARVTVRPGAAAWPYAARTDGPAYRAASSAFEDAWGVPPVQVGAGGTIPFVAA
                     FAEAFPEAEILMTGVEDPDTRAHGANESLHLGDFERACLAESLLLERLASRTP"
     gene            5643..6323
                     /locus_tag="VM638_01935"
     CDS             5643..6323
                     /locus_tag="VM638_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HVM11224.1"
                     /translation="MLDVREAPAPIRLTRRRRPDGRARVFTTLSQRDAAAWHRVAGRV
                     ARVIEPALGPGVLANRVRFSRASWRLEDLDEAIARARGAGRRLPRGLVLQTDVEAFYD
                     SVTPPLLAGSLLDAGLDPAEAARAADLLAGWRELGCPGLPVGPPGSAVMANALLLAVD
                     RSLGPRPFLRWVDDYRVPVASSQDAEAVLRRIDRALEGQGLRRSRTKTRIGPAEEIAF
                     PGSASGGA"
     gene            complement(6327..7205)
                     /gene="hisG"
                     /locus_tag="VM638_01940"
     CDS             complement(6327..7205)
                     /gene="hisG"
                     /locus_tag="VM638_01940"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HVM11225.1"
                     /translation="MLRFAVPKGSLEEQTLSLLEDADLRVRRGSSRDYHGRIDDERID
                     RVSLLRPQEIPRYVEEGFFDLGITGRDWVEETAARVEVLTELTYAKGGVGHPVRIVLA
                     VPIDSPAERAADLPPESRISTEYPELTKRHFERLGIPVRVFVSYGATEAKVPDIVDAI
                     VDVSETGGTLRAHGMKVIETLMESGVLLIANPAAAADPDKRRAMDDVRTLMEGAMAAR
                     GRVLIKLNVAADRLEGLLRVLPAMKAPTISSLAGEEAYAIETVVEKATVNTLIPLLKA
                     HGATDILEIPISKIVP"
     gene            complement(7205..7516)
                     /gene="hisI"
                     /locus_tag="VM638_01945"
     CDS             complement(7205..7516)
                     /gene="hisI"
                     /locus_tag="VM638_01945"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HVM11226.1"
                     /translation="MDLRFDEDGLIPAVVQDEENGEVLMVAWMNEEAVRRTLESGRTW
                     FWSRSRREMWRKGDTSGHEQHVREVRFDCDADTLLVKVHQVGAACHTGERTCFHRVLE
                     G"
     gene            complement(7516..8277)
                     /gene="hisF"
                     /locus_tag="VM638_01950"
     CDS             complement(7516..8277)
                     /gene="hisF"
                     /locus_tag="VM638_01950"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HVM11227.1"
                     /translation="MTLARRLIPCLDVDRGRVVKGTRFVDIRDAGDPVELASRYDAEG
                     GDELVFLDITASVEGRGAILDVVRRTADQVFIPLTVGGGVRSVDDVKALLRAGADKVS
                     LNTAAVEDPPLLARCAERFGTQCVVVAIDARRRNGGWEVATHGGRKPTGHDAVEWAAR
                     ATRELGAGEVLLTSMDRDGTGLGYDLDLLRAVGEAVPVPVIASGGAGALEHFAEALTE
                     GGADAVLAASRFHFGDFTVPDVKRYLAERDIPVRL"
     gene            complement(8274..8981)
                     /gene="hisA"
                     /locus_tag="VM638_01955"
     CDS             complement(8274..8981)
                     /gene="hisA"
                     /locus_tag="VM638_01955"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HVM11228.1"
                     /translation="MIVLPAIDVRGGRVVRLTRGVAGSEVVYADHPVEVGLRFVAEGA
                     SWLHVVDLDAALGTGSNPEVLRELIAAVPVPVQLGGGIRSLEAVEEALILGAARVVLG
                     TATVRDPGFLRSVSERFPDRVVTAIDTDGQDVLVRGWTEGAGALDELVPRLAEAGATR
                     FLATAVDRDGTLGGPDLDLYRRLTALASVPIQASGGVRSLDDLRALAGTAVEGAVVGK
                     ALYEGTLRLADALGATT"
     gene            complement(8978..9586)
                     /gene="hisH"
                     /locus_tag="VM638_01960"
     CDS             complement(8978..9586)
                     /gene="hisH"
                     /locus_tag="VM638_01960"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HVM11229.1"
                     /translation="MTRVAILDYGMGNLRSVARAIEHVGGKAIVATDPEGAAAADALV
                     VPGVGAFAACVERLRARDLDQAIRGAVDEAKPTLGVCLGMQILFEHGEEGDAEGLGLL
                     PGDVPRLPSTVKVPHMGWNTVEWVSEHALVRGVARGSRFYFVHSYVCRPRADVDVVGE
                     AEHGVRFPAVVARGSLVATQFHPEKSGEAGLRVYRNFIEAAA"
     gene            complement(9583..10164)
                     /gene="hisB"
                     /locus_tag="VM638_01965"
     CDS             complement(9583..10164)
                     /gene="hisB"
                     /locus_tag="VM638_01965"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HVM11230.1"
                     /translation="MTRHAEVRRTTKETDVAVRLTIEGSGSSAADTGIPFFDHMLQQL
                     GTHARFDLEVRAKGDLEVDAHHTVEDVGIAIGQALVEALGDKTGVRRFGHASVPLDEA
                     LVEVSLDLSGRPFVVHEVPVPAETIGTYDPGLTEDFVRAFATTAGMTLHARLRSGRSP
                     HHVVEAEFKAIARALGDACEIRGGGTPSTKGTL"
     gene            complement(10161..11228)
                     /gene="hisC"
                     /locus_tag="VM638_01970"
     CDS             complement(10161..11228)
                     /gene="hisC"
                     /locus_tag="VM638_01970"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HVM11231.1"
                     /translation="MRPRLRGDLGSLSAYLAPQIEAPIRLNTNESAHPLPPAFADDLA
                     AAVRGIEFHRYPDREAKDLRAALAAHTGHSAEGIWVANGSNEVIQQLCLAYAGPGRKV
                     LLFDPTYSMHSLIPRMVGAELAREPLPPGFWLETEGAVDAVERHDPAIVFVCSPNNPT
                     GNAQSLEVVDALCGAAPLVLVDEAYIEFGGETAQPLLERHENLVVVRTFSKAFSLAAA
                     RLGYCLCAPALLEDLARVRLPYHLSSLTQAAGLAALRHLGEAEAILTTIAARRDRLFE
                     RLDRMPGVQPSRSQANFVLFRTDVEARALWEALLRRGILVRDVSNYPMLERCLRVTAG
                     TDEEIDAFIEALPDCFEEVVP"
     gene            complement(11225..>12265)
                     /gene="hisD"
                     /locus_tag="VM638_01975"
     CDS             complement(11225..>12265)
                     /gene="hisD"
                     /locus_tag="VM638_01975"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HVM11232.1"
                     /translation="PADLERALEAAAERLRDLHGRQVPVAWEEERDGVRFGEVVRPVA
                     GAGCYVPGGRASYPSTVLMTAIPARVAGVGRVVVCTPPAEDGSLAPAVLVAARLAGVD
                     DVFRVGGAQAVAALAFGTRTVPRVDVIVGPGNAWVTAAKREVSGQVGIDGLAGPSELV
                     VVADGSIPPPVLAADIVAQAEHDPQASAVLICLDPDLPDAVLAALEEEVRRSPRRGIV
                     ERALEHAVIAIADEDGAVDVVDRLAPEHLQVATSDPRRFVRRVRSFGAAFLGPHTPVS
                     FGDYGVGSNHVLPTMTTARFTSGLRADRFVTVSSVVEATAEAAKLLGPDIERIASAEG
                     LAAHARASEVRR"
BASE COUNT         1700 a         4363 c         4473 g         1729 t
ORIGIN      
        1 gctctctcgg tgatgacgca ggacctcccc gcaacggccg acgtcgtcgt cgccggcggc
       61 ggcgtgatgg gggcgagcac cgcgtatcac ctcgcgcgga tgggcgcggg gcgtgtcgtc
      121 ctcgtcgagc gtgagggcta cctcggagag atgtcgaccg gccagtgcgc cggcgggatc
      181 cggcaccagt tctccaccga gacgaacgtt cggctctcga aggaatcgat cgcgatgatg
      241 gagcggttcc ccgaggagct gggccagccg ctcgacatcg acctctgcgg ctacctgatc
      301 ctgctcaccg acgagcggga tgtggccgtc ttccgcgaga acgtcgcgct tcagcgccga
      361 ctcggcgtcg agaccgagtg gctcgacccg gagcaggttc acgagcggat cccgctcgtg
      421 aacaccgacg gcgtgctggc cgcgacctac tacgaccgtg acggcctggc ggacccgggt
      481 ggtgtggtcc agggatacgc gaccgctgca cgaggactcg gcgtgacgat cgccaccggg
      541 tgcgaggtca ccggcatcga gaccgcgtca gggcgcatcc gagccgtcga gaccacacga
      601 ggcaggatcg agacgccggt cgtggtgaac gcctgcggcg cctgggcgcc ccacctgggc
      661 gccatggtcg gcgtcgagat cccgatccga cccatccggc gccagatcgt ctcgaccacc
      721 ccgatcccgg gcctgccgcc cgacttcccc ttcgtcgtct tcttcggcac gtcgctctac
      781 ctccaccgcg aaggcgaggg gatcctcagc ggcaagtcca acaacgacga ggagccgggg
      841 ttcaagctcg aggtggacga ggagtgggag gccgtccaca tgatggagct gatggagcgg
      901 ttcccggcgg tcgaggaggc cgggatccgc gcgcgctggg cggggctgta cgaggtcacg
      961 cccgacgccc atccgatctt cggccgggtc tcgccggtcg aggggttcta cgtcatggcc
     1021 gggttctcgg gccacgggtt catgcacggc ccggtgatgg gcctgctcat ggccgaggag
     1081 atcctcgagg gagaagcgcg gacggtcccg gtcggggagt tccgctacga gcggttcctc
     1141 accggcgacc tgagccccga gtacaacgtg atctagccga ggagggggcg gaccttcgtt
     1201 ccctcggggg tgtccatgac ctcccagccc atgtcggtca ggcggccgcg gatctcgtcg
     1261 ctcctcgcgt agtccttctc cgcccgggcg gcgtcgcgtt ccgccatgag tgagcggacg
     1321 tccccgggga tctcgaatcc ttcgcgggcg agcttgccga agtcgaggcc gaggaagcgg
     1381 tcccagtcct cgagcaggcg ccgcttgtcg gtgtcgggga cgtcggccga gagcgtctcg
     1441 ttcagcaccg caacggccgt cggggtgtcc aggtcgtcgg cgaccgcctc gcggaaccgg
     1501 cggtcgaggt cctgggcccc ggagccgagc tctgcgctcg aaccgtccca cccggccagg
     1561 cgctggcgga tctgggtcag cttccggtcg gcggcgtcga gcgcctccca gcggaagtcg
     1621 aggacgctgc ggtaccgcgt ctggaggcag aggtagcgga acgcgagcgg atcgtggcca
     1681 cgcgcgacca cgtcgtcgat cgtgtagacg ttccccttcg acttcgacat cttctggccc
     1741 tcggccagca ggtgcccgcc gtggacccag atcgagatga cctggtggcc gagcgcggcg
     1801 tcggactggg cgatctcgtc ctcgtgatgg gggaagacga ggtcggtccc gccggtgtgg
     1861 atgtcgaacc gctccccgag gtacttcacc gacatcgccg agcactcgat gtgccagccg
     1921 gggaacccct cgccccaagg gctgtcccac ttcatgaggc ggttcggtgc ggcgggcttc
     1981 cacagcgcga agtcggcgtg gtgacgcttc ccctcgccgg cctcgaggtc gcgatggccg
     2041 ggctcgaggt tctcgagcgt gttccgcgac agggcgccgt aggccgggaa cgaccgcacg
     2101 tcgtagtaga cggtcccgtc atcgagctcg tacgcgtggc cacggtcgat cagggtgcgc
     2161 gtcatatcca gcatctcggg gatgtggtcg gtggcccgcg ggtactcgtg ggcgggcagg
     2221 gccccgatct tcgccgtgtc ctcgaggaac atccgcgtgt acttctcggc gatctcccat
     2281 ggaccgagcc cctgctcctc catcgcgagc tccatccggt cctggtcggt ggcgtcgtcg
     2341 accatgtgcc cgacgtcggt gatgttgatc acctgcgtca cgcggtggcc ctcgagctcg
     2401 aggccgcgtc gaacgaggtc ggagagcagg aagctgcgga ggttcccgat gtgcaccggc
     2461 cggtagacgg tcggaccgca cgtgtacatc ttcacgtgcc cttcctcgac cgggcggacg
     2521 tcgtcgaccc gccgggtgag ggtgttgaag aggcgcacgg ccgacaggat aggctatggg
     2581 tcggagggag cgagatggat cgggacttcg gcctcgacga cgaacagcgg gagatcgtcg
     2641 gcctcgtgcg ggagttcgtt gccgagcggg tcgccccgca gtcggccggc tacgaggagc
     2701 gcaaggagtt ccctcgcgag gcgttccggg ccctcggcga gatggggctg ggcggcatcc
     2761 cctacgacga gagtcacggc ggcgggggac agccgttcct cacgtacctg gcggtgctcg
     2821 aggagatcgc ggcgggccac gtcgcgctgg cggtcgggct gtcggtccac cacctttccg
     2881 cgttcggcgt cgagcgctac ggctccgacg acctgaagca gcggtacctc ccgcggctgt
     2941 tctccggtga gtggctgggc gcttatgcgc tctccgaggc ctccagcggc tccgacgccg
     3001 cggccctgcg gacgcgcgcg gcccggaccg atgccggctg gaggttggac ggttcgaagc
     3061 ggttcatcag ccacgcgggg gaggcggagt tctacctcgt gatggcgcgg accggtgagg
     3121 acggcccgaa gggcatcagc gcgttcgcgg tcgagaagga ttggccgggc ttcggattcg
     3181 gcaagctcga ggagaagatg ggctggcacg cctcgcccat gagcgagctc ctgttcgacg
     3241 gatgcgacgt gccgaccgag aacctcgtcg gcgaggaggg gcggggcttc gaggtggcgc
     3301 tcgcggcgct cgactccggc cggctcggga tggcggcctg cgccgtcggg gtcgcgcaag
     3361 cagccctcga ggcggcgatc accttcacga aggaccggac ccagttcggg aagccggtca
     3421 tcgagttcca gggcctccag ttcatgctgg ccgacatggc cgtgcaggtg gaggcggcgc
     3481 ggcgcctgta ccgcgaggcg gcgcgggcgc gcgacgcggg acagggcacc tccgtgtcgt
     3541 gctcgatggc gaaggtcttc gcgtccgaca tcgcgatgcg cgtcacgacc gatgccgtcc
     3601 aggcctgcgg cggctacggc tacatgcagg agtatccgct cgagcgctac atgcgcgagg
     3661 cgaaagccct gcagatcgtc gagggcacga accaggtcca gcgcatgatc atcgggcgcc
     3721 acctggcgcg atgacggagc acgtggacca gcccccgttc gatgtgatcg tctccggcag
     3781 gcgacacctg ctcggcgccg gcgcgggcgc gtacgaggtc tgggagaaag ggcgagaagg
     3841 cgagaccctg gcgagcttcc cgctcaccga cgcggggctg cgtcaggcgg aggcggcgtt
     3901 cgacgagctc gaacggcggg gccggtggtc ccgtccccgg gtggaagccg cgctgaggac
     3961 ggcgctcttc tggggtctcg gcgcgtggct cgtgatcggc ctcgtcgagg tgatctgggg
     4021 catcgtccgc gacttcgccg cgatcccacc cgagatcggg ttctcggagc cggggacgcc
     4081 gtggctccgg atcgtgctcc aggtgctgtc ggtgacggcg ttccggatct gggtcgcggc
     4141 actggttgcc ctcgccgcgc tccgctggct cgttcccacg ggcaccgaag cacggccgtg
     4201 accgtcgccg agcggctgac cggcgtactg ccgcaggtcc gtcaggacct cgagcgcctg
     4261 gtgcggatcc cgtcggtgag cgctcccggg ttcgacccgg aggaggtggt ccgagccgcg
     4321 accgccaccg ctgaggtgct cgaggcatcc gggctcggcg cggtacggat cctggagatg
     4381 gagggggagc acccggcggt ctacggtgag acgcccgcgc aggatggagc gccgaccgtc
     4441 ctcctgtacg cgcaccacga cgtccagccg cccggacgac ccgaggactg gatgtcgccg
     4501 ccgttcgagc cgacggagcg cgccggtcgc ctgtacggca ggggaacggc cgacgacaag
     4561 gccgggatcg cggtgcacgc cgcggcgtta cgcacgcacg aggggtcgcc gccggtcggg
     4621 gtcaaggtcc tgatcgaggg cgaggaggag atcggctcgc cgaacctgcc cggtttcctc
     4681 gagcggtacg gggaggacct gcgagcggac gtgatcgtcc tcgccgacat ggtgaactgg
     4741 cgcgtcggcc agccggcgct cacgacctcg ctgcgcggcc tcgtcgactg ctacgtcgag
     4801 gtgcggaccc tcgagcacgg gatccacagc ggcatgttcg gcggcgcctt cccggacgcg
     4861 ctcagcgtcc tctgccggat cctcgcgacg ctccacgacg acgacggcag cgtcgcggtc
     4921 ccgggtctgt cgtctggccc ggcggacgag ctcgacctca cggaggcgga gctgcgggcg
     4981 caggcgggcg ccgtcgaggg ggtcgcgctg atcggctggg ggagcctgac ggagcgggtg
     5041 tggacgatgc cggcggtctc ggtgctcgcg atcgatgctc cgcccgtggc ggaagcctcg
     5101 aacacgctcg tgccggccgc tcgcgcgaag gtgagcctcc ggatcgcccc cggtgactcg
     5161 ccggaggagg cgatgcgcgc cctcctcgac cacctgcgct cgaacgcgcc ctggggcgct
     5221 cgtgtcacgg tcaggcccgg ggcggcggca tggccgtacg ccgcgcgcac cgacggtccg
     5281 gcgtaccgcg cggcctcgtc agccttcgaa gatgcgtggg gcgtcccgcc ggttcaggtc
     5341 ggggccgggg ggacgatccc gttcgtcgcc gctttcgccg aagcgttccc cgaggcggag
     5401 atcctgatga ccggcgtcga ggaccccgac acgcgcgcgc acggggccaa cgagagcctg
     5461 cacctgggcg acttcgagcg ggcgtgcctc gccgagtccc tcctgctcga gcgcctcgct
     5521 tccagaaccc cctgacgggc agcccccttc ccccgggtgg gtgatgcgcc cggccgccgc
     5581 gccgcggtag aaccctgatg tcgccgatgg ccaacctcct cctggccggg aggagggaag
     5641 ccatgctcga cgtccgcgaa gcacccgcac cgatccgctt gacccggcgc cgccgcccgg
     5701 acggccgggc gcgcgtcttc accacgctct cccagcggga tgctgccgcc tggcatcggg
     5761 tcgccggccg ggtcgcgcgg gtgatcgagc ccgcgctcgg gccgggtgtg ctggccaacc
     5821 gcgttcgctt ctcgcgcgcc tcgtggcggc tcgaggacct ggacgaggcg atcgcgcggg
     5881 cgcggggcgc cggccgccgg ctgccgcgtg gcctggtcct gcagaccgac gtggaggcgt
     5941 tctacgactc cgtgaccccg cccctgctcg ccgggagcct cctcgacgcc gggctcgatc
     6001 cggccgaggc tgcccgcgcc gccgatctgc tcgccggatg gcgggagctc ggttgcccgg
     6061 gactgcccgt gggtccgccg gggtcggccg tcatggcgaa tgccctcctg ctggcggtgg
     6121 atcggtccct cggcccgcgg cccttcctcc ggtgggtgga cgactaccgc gtcccggtcg
     6181 cgtcctcgca ggatgccgag gccgtcctcc ggcgcatcga ccgggcgctc gagggtcagg
     6241 gtctccggcg gtcccgcacg aagacccgga tcgggccggc cgaggagatc gccttcccgg
     6301 ggagcgcgtc gggcggcgcg tgagcgtcag ggcacgatct tcgagatcgg gatctcgagg
     6361 atgtcggtgg cgccgtgcgc cttgaggaga gggatgagcg tgttcacggt ggccttctcg
     6421 acgaccgtct cgatcgcgta cgcctcctcg cccgcgagcg aggatatcgt cggcgccttc
     6481 atcgccggca gcacgcgcag cagcccctcc aggcggtcgg cagcgacgtt cagcttgatc
     6541 aggacgcgtc cgcgcgcggc catcgctccc tccatcagcg tccgcacgtc gtccatcgcg
     6601 cggcgcttgt cggggtcggc ggcagcggcc gggttcgcga tgaggagcac gcccgactcc
     6661 atcagcgtct cgatcacctt catgccgtgc gcccgcaggg ttccgcccgt ctcggagacg
     6721 tcgacgatcg cgtcgacgat gtccggcacc ttcgcctcgg tcgccccgta cgagacgaag
     6781 acgcgcaccg ggatgccgag acgctcgaag tgacgcttcg tgagctccgg gtactcggtc
     6841 gagatgcggc tctcgggggg gaggtcggcg gcgcgctcgg cgggcgagtc gatcgggacc
     6901 gcgaggacga tccgcaccgg atgcccgaca ccacccttcg cgtaggtcag ctccgtcagg
     6961 acctccaccc gcgcggccgt ctcctcgacc cagtcacggc cggtgatgcc gaggtcgaag
     7021 aacccctcct cgacgtagcg cgggatctcc tgggggcgca ggagggagac ccggtcgatc
     7081 cgctcgtcgt cgatccgtcc gtggtagtcg cgcgaggatc cgcggcgcac acggaggtcg
     7141 gcgtcctcga ggagcgagag cgtctgctcc tcgagcgatc ccttcggcac cgcgaagcgc
     7201 agcatcagcc ctccagcacc cggtggaagc aggtgcgctc acccgtgtgg cacgcggcgc
     7261 cgacctgatg gaccttcacg agcagcgtgt ccgcgtcgca gtcgaagcgc acctcgcgga
     7321 cgtgctgctc gtggccgctc gtgtcgccct tccgccacat ctcccggcgc gagcggctcc
     7381 agaaccaggt ccggccggac tcgagcgttc gccggaccgc ctcttcgttc atccacgcga
     7441 ccatcaggac ctcgccgttc tcctcatcct ggaccaccgc cgggatcagc ccgtcctcgt
     7501 cgaaccgcag atccatcaca gcctcaccgg gatgtcgcgc tcggcaaggt accgcttcac
     7561 gtccggcacc gtgaagtcgc cgaagtggaa gcgggaggcc gcgaggacgg cgtcggcgcc
     7621 gccctcggtc agcgcttcgg cgaagtgctc gagcgcgccc gcgccgcccg aggcgatcac
     7681 cgggaccggg accgcctctc cgaccgcccg caggaggtcc aggtcgtagc ccaggccggt
     7741 cccgtcgcgg tccatcgagg tcagcaggac ctccccggcc ccgagctcgc gcgtcgcccg
     7801 ggccgcccac tcgaccgcgt catgaccggt cggcttccgg cctccgtgcg tcgccacctc
     7861 ccacccgccg ttccggcggc gggcgtcgat cgcgaccacc acgcactggg tcccgaagcg
     7921 ctcggcacag cgggccagga gcggcgggtc ctccacggcg gcggtgttga gcgacacctt
     7981 gtcggccccg gcgcgcagga gggccttgac gtcgtccacc gagcgcacac cgccgccgac
     8041 cgtgaggggg atgaagacct ggtccgcggt ccggcgcacg acgtcgagga tcgcgccgcg
     8101 gccctcgacg ctcgcggtga tgtcgaggaa gaccagctcg tctccaccct cggcgtcgta
     8161 ccgggaggcg agctcgaccg ggtcgcccgc atcgcggatg tcgacgaagc gcgtgccctt
     8221 caccacgcgt ccccggtcga cgtcgaggca cgggatcagc cgtcgtgcga gcgtcacgtc
     8281 gtcgctccga gcgcgtcggc caggcgcagc gtgccctcgt agagcgcctt gcccacgacc
     8341 gctccctcga cggcggtccc ggcgagcgcg cgcaggtcgt cgaggctccg caccccgccg
     8401 gacgcctgga tcgggacgga ggcgagtgcg gtgagccgcc ggtagaggtc gaggtcgggc
     8461 cccccgaggg tcccgtcgcg gtcgacggcg gtcgcgagga agcgggtcgc accggcctcg
     8521 gcgagccggg gcacgagctc gtcgagcgct cccgcgccct ccgtccaacc gcggacgagc
     8581 acgtcctgcc catcggtgtc gatcgccgtc accacgcgat cggggaaccg ctcggagacc
     8641 gaccggagga acccggggtc tcggacggtg gcggtcccga ggacgacgcg cgccgccccc
     8701 aggatcaacg cctcctccac cgcctcgagc gagcggatcc ccccgccgag ctggaccggc
     8761 acggggaccg cagcgatcag ctcgcgcaga acctccgggt tcgaaccggt gccgagcgcg
     8821 gcgtcgaggt cgacgacgtg gagccacgac gcgccctcgg ccacgaaccg caggccgacc
     8881 tcgaccggat gatccgcgta cacgacctcg ctgccggcga cgccccgcgt caggcggacg
     8941 acccgaccgc ccctgacgtc gatcgcggga aggacgatca tgcggccgcc tcgatgaagt
     9001 tccggtagac gcgcaggccc gcctcacccg acttctcggg gtggaactgc gtcgcgacga
     9061 gcgacccgcg cgccacgacg gcggggaacc gcacgccgtg ctccgcctcg ccgacgacgt
     9121 cgacgtcggc gcgggggcga cagacgtagg agtggacgaa gtagaagcgg gatccccggg
     9181 cgaccccgcg cacgagggcg tgctcggaca cccactccac cgtgttccag cccatgtgcg
     9241 gcaccttcac cgtcgagggg agccggggga cgtcaccggg caggagaccg agcccctcgg
     9301 catcgccctc ctcgccgtgc tcgaacaaga tctgcatccc caggcagacg ccgagggtcg
     9361 gcttcgcctc gtcgacggct ccgcggatcg cctggtcgag gtcccgggcc cgcaggcgct
     9421 ccacgcacgc ggcgaacgcg cccacgcccg gcacgacgag cgcgtcggcc gccgcggccc
     9481 cctccggatc ggtcgcgacg atggccttcc cgccgacgtg ctcgatcgct cgcgcgaccg
     9541 aacggaggtt gcccatcccg tagtcgagga tcgcgacccg cgtcacaggg tgcccttcgt
     9601 cgagggcgtg ccgccgccgc ggatctcgca ggcgtcgccc agggctcgcg cgatggcctt
     9661 gaactccgcc tcgacgacgt ggtgcgggct ccgccccgat cgcagccgtg cgtgcagggt
     9721 catcccggcg gtggtcgcga aggcgcggac gaagtcctcg gtcagtccgg gatcgtaggt
     9781 cccgatcgtc tcggcgggaa cgggaacctc gtggacgacg aacggccgtc ccgacaggtc
     9841 gagcgagacc tcgacgagag cctcgtcgag cgggacggac gcgtgtccga aacggcggac
     9901 gccggtcttg tcaccgagcg cctcgacgag ggcctgcccg atcgcgatgc cgacgtcctc
     9961 gacggtgtgg tgcgcatcga cctcgaggtc tcccttcgcc ctcacctcca ggtcgaaccg
    10021 cgcgtgcgtc cccagctgct ggagcatgtg gtcgaagaac gggatcccgg tgtcggcggc
    10081 cgacgacccg gagccttcga tcgtcaggcg caccgcgaca tcggtctcct tcgtcgtccg
    10141 gcggacctcg gcgtgacgcg tcatgggacc acctcctcga agcagtcggg gagcgcctcg
    10201 atgaacgcgt cgatctcctc gtcggttccg gcggtgacgc gcaggcagcg ctcgagcatc
    10261 gggtagttcg agacgtcgcg gacgaggatg ccgcggcgca gcagcgcctc ccagagcgcc
    10321 cgcgcctcga cgtcggtccg gaacaggacg aagttggcct gcgagcggga cggctgcacg
    10381 cccggcatcc ggtcgaggcg ctcgaacagg cggtcgcggc gcgcggcgat cgtcgtgagg
    10441 atcgcctcgg cctcgcccag gtgccgcagg gcggcgaggc cggcggcctg cgtgagcgac
    10501 gacaggtgat aggggagccg gacgcgcgcg agatcctcca ggagcgccgg cgcgcagagg
    10561 cagtacccca ggcgggcggc ggcgagggag aaggccttgg agaaggtgcg gaccacgacg
    10621 aggttctcgt gccgctcgag cagcggctgt gccgtctctc cgccgaactc gatgtatgcc
    10681 tcgtccacga gcacgagcgg tgccgcgccg cacagcgcgt cgacgacctc gagcgactgg
    10741 gcgttccccg tcgggttgtt gggggagcag acgaacacga tggccggatc gtgccgctcg
    10801 acggcgtcca ccgcaccctc cgtctccagc cagaacccgg gcggcagggg ttcgcgcgcc
    10861 agctcggctc cgaccatccg tgggatgagg gagtgcatcg agtaggtggg atcgaacagc
    10921 aggaccttcc tcccggggcc ggcgtaggcg aggcagagct gttggatcac ctcgttcgac
    10981 ccgttcgcca cccagatccc ctcggcgctg tgccccgtgt gcgccgcgag cgccgcccgc
    11041 aggtccttcg cctcgcggtc ggggtaccga tggaactcga tgccgcggac cgcggccgcc
    11101 agatcatcgg cgaacgccgg agggagggga tgcgcggact cgttcgtgtt cagccggatc
    11161 ggcgcctcga tctggggagc gaggtaggcc gagagtgacc cgaggtctcc gcgcaggcgc
    11221 ggtctcaccg tcggacctcg ctcgcccgcg catgggcggc gagaccctcg gcggaggcga
    11281 tccgctcgat gtcgggcccc aggagcttcg ccgcctccgc cgtcgcctcg acgacgctcg
    11341 agacggtgac gaagcggtcc gcgcggagac cggaggtgaa gcgcgccgtc gtcatcgtcg
    11401 gcaggacgtg gttggatccg acgccgtagt ccccgaacga gaccggcgtg tgcggcccga
    11461 ggaaagccgc gccgaacgac cgcacccgcc gcacgaagcg gcgtgggtcg gacgtcgcca
    11521 cctgcaggtg ctcgggagcg aggcggtcga cgacgtcgac ggcgccgtcc tcgtccgcga
    11581 tcgcgatgac ggcatgctcg agcgcccgct ccacgatccc gcgtcgcggt gacctccgga
    11641 cctcctcctc gagcgccgcg aggacggcat ccggaaggtc cgggtcgagg cagatgagca
    11701 ccgcggaggc ctgggggtca tgctccgcct gcgcgacgat gtcggcggcg agcaccggcg
    11761 gggggatcga gccgtccgcg acgaccacca gctcgctcgg acccgcgagc ccgtcgatcc
    11821 cgacctgacc ggagacctcg cgcttggccg ccgtcaccca ggcgttcccc ggcccgacga
    11881 tcacgtcgac gcgtggaacc gttcgggtcc cgaacgcgag ggccgcgacc gcctgcgcgc
    11941 cccccacgcg gaagacgtca tcgacgccgg cgaggcgcgc ggccacgagg accgcgggag
    12001 cgagcgagcc gtcctcggcc ggaggcgtgc agacgacgac ccgccccacg cccgccaccc
    12061 gcgccgggat cgccgtcatc agcaccgtcg acgggtacga cgcacgcccg cctgggacgt
    12121 agcacccggc gcccgcgacg ggccgcacga cctcgccgaa gcgcacgcca tcgcgctcct
    12181 cctcccacgc gaccgggacc tggcgtccgt ggaggtcgcg gagccgctcc gccgccgcct
    12241 ccagcgcgcg ctcgaggtcc gcggg
//
LOCUS       DATLGG010000074         8601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_37444, whole genome shotgun sequence.
ACCESSION   DATLGG010000074 DATLGG010000000
VERSION     DATLGG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37444"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VM638_01980"
     CDS             complement(<1..351)
                     /locus_tag="VM638_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM11233.1"
                     /translation="MSDVPFAAQVVEWFADAGRWSGTTGIPNRLGEHVLMSLLAVAIA
                     TAVALPAGLLIGHARRFELVVVSVANVGRAVPSFGLLFLFVLWLGLGLRYPAWGRPPV
                     VLALVLLAIPPILTN"
     gene            complement(348..989)
                     /locus_tag="VM638_01985"
     CDS             complement(348..989)
                     /locus_tag="VM638_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVM11234.1"
                     /translation="MRAWEWFAANTDLVMERLLEHVGLTGAAVGAGIAIALPLAILAH
                     RRPRLAGPILWTTGILYTIPSLAALALLLPLLGLSAANAIVVLTTYTLLILVRNTVTG
                     LREVPADVRDAARAAGHTPRQLLWRVELPLALPAIVAGIRIATVTTIGLVTVTALIGK
                     GGLGYLMLAGFRPLLPEAAIVVGIVLSVGLALTADLLLVGLQRALGPWAGRRR"
     gene            complement(986..2083)
                     /locus_tag="VM638_01990"
     CDS             complement(986..2083)
                     /locus_tag="VM638_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM11235.1"
                     /translation="MISLERVTKVFAGSDRPAVQELSLEIPEGEVVALVGPSGCGKTT
                     TLRMVNRLVEPTSGTIRVAGTDVMAQDPVALRRGIGYVIQSVGLLPHRTVEQNIATVP
                     RLLGWDRTRTGVRVRELATLLELDEQLLPRYPHELSGGQQQRVGVARALAADPPVMLM
                     DEPFAAVDPVVRGRLRAQFVALQARLRKTIVIVTHDIDEAIAMADRIAVLSTGATVEQ
                     YAPPEEVLRDPANAFVAGFLGSERGLKRLALTRVGEISLGEGPVVGSTDPAKVALGAM
                     AAVGSDWTSVVDGDGRLLGWVDHASLGGAGRVDDVRPQPFSASITASSTLREALDAIV
                     SSRTGVAAVVDERGRYRGILTLDGVTRAIAR"
     gene            2242..3372
                     /locus_tag="VM638_01995"
     CDS             2242..3372
                     /locus_tag="VM638_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11236.1"
                     /translation="MPATFDCGPDDGRERLTTDGYAFDFEAPHLTAPELASPVPANHP
                     AVTAQRSALFFLRADLAPYRSGDLEISLTWPETSDYDIFVFDAQGTEIARSAASNIDG
                     GDTRFERALVEGIAHCADLSIAVRSWAGRPDQTLSLRIAFGNPGPMFACEKDDPHPAC
                     EGKEEGEPPARLPDTRTRLYLGGDRPGQLSMQGHYVHDLEVNGIRPVGGEAELSSHLV
                     PVRPTGGRPNHFTKLVAGNPEQHENPFMANYSLQLKQPTRITGDVKALIWVSSQTFGQ
                     GGGGGPLFVDLWQDGTAALAGTRIARVEVPGSKIGAAPTPVRVTFSGVDALVERELVL
                     QVSSEPVAGTSGGSQDPADSEWTIYYDSVQFPAHLTLNLPQA"
     gene            3400..4044
                     /locus_tag="VM638_02000"
     CDS             3400..4044
                     /locus_tag="VM638_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVM11237.1"
                     /translation="MAPMFLDVTSDNPFASNCWAVSADGSDEAVVVDPGFEPEVIARM
                     LERAGKRLVAVLATHGHFDHIGAVPQVCAAGVPVVIHEADELALSDQAAWGSGHPVPM
                     DLRPKDVITVSGGDRLEYAGFSFEVMHTPGHTPGSACFVTDGFLFSGDLLFRGSIGRH
                     DFPNSSARDMRRSLGRFLALPDRLRVYPGHGPETTVGDERVSNPWLVELAGARE"
     gene            4041..5297
                     /gene="hisS"
                     /locus_tag="VM638_02005"
     CDS             4041..5297
                     /gene="hisS"
                     /locus_tag="VM638_02005"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVM11238.1"
                     /translation="MTDLNPPRGTQDLLPPRSDAVEALADEGRRLARRFGFRRVETPT
                     FEHTELFARGVGASTDIVTKEMYTFEDKGGRSLTLRPEGTAPVMRAYLAHKQELGAPF
                     KAYYVVPMFRHERPQAGRMRQHTQLGVEVMGTAAPAADVEVVTLGQRFLSGRGLRRFE
                     LRLNSIGDEVCRPAYRTALIDFLKERVDRLCRDSRERMLQNPLRVLDCKVPSCRAATE
                     DAPRITDHLCDACREHFDAVQAGLREEGITFVLDHRLVRGLDYYTRTTFEFVSEVLGP
                     TQSTLCGGGRYDGLAELLGGEPTPGIGFGLGLERLLIAIDEEGVPLPEPEPLAVFVVA
                     LGEEAAAEARALVRELREEGLAAAGSLEDRPMKAQMKMADRAGARYVAIIGEQELADG
                     VVTMRRMADGEQESVKRSEVPGWISR"
     gene            5282..7030
                     /gene="aspS"
                     /locus_tag="VM638_02010"
     CDS             5282..7030
                     /gene="aspS"
                     /locus_tag="VM638_02010"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HVM11239.1"
                     /translation="MDLPLRTGYCGGLRAEDAGREVALCGWVARSRDHGGVVFIDLRD
                     REGIAQVVFHPQDAPEAHAVAADLKAESVVRVLGEVRERPEGTRNPNLPTGDVEVAAR
                     EVEVLSVAETPPFPVEDDVQADERLRLRHRYLDLRRPEMTHTLRLRHAAFSAIHRFMD
                     EHGFVEVETPMLTRSTPEGARDFLVPSRLQEGTFYALPQSPQIFKQILMVSGLDRYYQ
                     VVRCFRDEDLRADRQPEFTQLDVEMSFVNEEDVHAVVEEMLAAVWREVLDVEIATPFP
                     RLTYDEAMRRYGSDKPDTRYGMELADLTETFRGTGFRAFASVIDGGGVVKGFAAPGAA
                     SWSRKELDDLVVEAQGRGASGLVWVAFAGDEVRSPVAKFLSEGELAALRTETGAGDGD
                     LALVVADAEGRANTVLDGLRRLMADRLGLVPEGRWDFLWITEPPVFEWSEEDERWVSV
                     HHPFTSPATDDLSPETAKARAYDVVLNGWELGGGSIRIHRPDVQRAVFEALGMSNEEA
                     EEKFGFLLDAFRYGVPPHGGIAFGLDRMTMLLAGRSNIREVIAFPKTQSGTDLLTGAP
                     AEVADDQLRELGIRLR"
     gene            7965..8411
                     /locus_tag="VM638_02015"
     CDS             7965..8411
                     /locus_tag="VM638_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11240.1"
                     /translation="MCRISEQEEDPTQPAPKEQTDPGLDEELIQPGPDEEPVVDPSPE
                     RDGGSTTPVRPGPGVPAGRSSTPAIAPGAAPAAASAHGPDTGGSTGRDTSSRDYHPAI
                     DTPRGSLPVPPVGASGMLGAAFGLLMLAGAGGGLGAALLRLRARLA"
BASE COUNT         1216 a         3079 c         3152 g         1154 t
ORIGIN      
        1 gttcgtgagg atcggcggga tcgcgagcag cacgagggcc aacacgaccg gtggtcgccc
       61 ccacgcgggg taccggagcc ccagccccag ccagaggacg aacaggaaca gcagtccgaa
      121 ggacggaacc gcgcgaccga cgttggccac cgagacgacc accagctcga agcgccgggc
      181 gtggccgatg aggaggccgg cggggagggc gacggccgtc gcgatcgcaa ccgccagcag
      241 ggacatcagc acgtgctcgc cgaggcggtt ggggatcccg gtcgtgccgg accagcgtcc
      301 ggcgtccgcg aaccattcga cgacctgcgc ggcgaagggc acgtcgctca ccggcgcctc
      361 cctgcccacg ggccgagcgc gcgctgcagc cccacgagca ggagatcggc ggtgagcgcc
      421 aggccgaccg agagcacgat accgacgacg atcgccgcct ccggcaggag cggccggaat
      481 cccgcgagca tcaggtaccc gagccctccc ttcccgatca gcgccgtcac ggtcacgagc
      541 ccgatcgtcg tcacggtcgc gatgcggatc ccggccacga tcgccggcag cgcgagcggc
      601 agctcgacgc gccacaggag ctggcggggc gtgtgccccg cggcccgagc cgcgtcgcgg
      661 acatcggcgg gcacctcccg caggcccgtc accgtgttcc gaacgaggat cagcagggtg
      721 taggtcgtga ggacgacgat cgcgttggcg gctgagaggc cgagcagggg caacaggagg
      781 gcgagcgccg cgaggctcgg gatcgtgtag aggatccccg tcgtccacag gatcggtccg
      841 gccagccgcg gccgccggtg ggcgaggatc gcgagcggca gggcgatggc gatgccggcg
      901 ccgacggcgg cgcccgtgag tccgacgtgc tcgagcaggc gctccatcac gaggtccgtg
      961 ttcgccgcga accactccca ggccctcatc gtgcgatcgc ccgcgtcacg ccatcgagcg
     1021 tgaggatgcc gcggtagcgt ccccgctcgt cgacgacggc cgcgaccccg gtccggctcg
     1081 agacgatcgc atcgagggcc tctctcaagg tgctcgaggc ggtgatcgag gcgctgaagg
     1141 gttgcggtcg cacgtcgtcg acccgcccgg cgccgcccag cgaggcgtgg tccacccacc
     1201 cgaggaggcg tccatccccg tcgaccacgc tcgtccagtc ggacccgacg gccgccatcg
     1261 cgccgagcgc caccttcgcc gggtccgtgc ttccgacgac cggcccctct ccgagggaga
     1321 tctcccccac ccgcgtcagc gcgagccgct tcaaccctcg ctccgagccg aggaagcccg
     1381 cgacgaacgc gttcgccgga tcgcgcagca cctcctcggg cggcgcgtac tgctcgacgg
     1441 tggcacctgt gctgaggacc gcgatccggt ccgccatcgc gatcgcctca tcgatgtcgt
     1501 gggtgacgat cacgatcgtc ttccgcaggc gggcctgcag cgcgacgaac tgcgcccgga
     1561 gccggcctcg gacgacgggg tccaccgccg cgaacggctc gtccatcagc atcaccggcg
     1621 ggtcggccgc gagcgcgcgc gcaacgccca cccgctgctg ctgtcccccg gagagctcgt
     1681 gcgggtaccg cgggaggagc tgctcgtcga gctccaggag ggtggcgagc tcccgcaccc
     1741 gcactccggt cctggttcgg tcccagccga ggagacgggg caccgtcgcg atgttctgct
     1801 cgaccgtccg gtgggggagc aggccgacgc tctggatcac gtagccgatc ccgcggcgga
     1861 gcgcgaccgg gtcctgcgcc atcacgtcgg tcccggcgac gcggatcgtc ccggaggtcg
     1921 gctccaccag gcggttgacc atgcggaggg tcgtcgtctt cccacagccg gagggcccca
     1981 cgagcgcgac gacctccccc tccgggatct ccagcgagag ctcctggacg gccggccgat
     2041 cgctccccgc gaagaccttc gtcaccctct cgagggagat catccgctga gtatcgccca
     2101 gggcggctga aaaaagatcg ccggggaggc agggacccga cccccggatg ccgaataggg
     2161 gtggagatgc gacgctcgcc aaccttcgca tcgctggtgg ctttcgcggc tcttgttctc
     2221 catgcgcctg ccgcaggagc gttgccggcg acgttcgact gcggtcccga cgacggccgg
     2281 gagcgcctga ccaccgacgg ctacgccttc gatttcgaag cgccccatct gaccgcgccc
     2341 gagctcgcct ctcccgtgcc cgcgaaccac cccgccgtga cggcccagcg ttccgccctc
     2401 ttcttcttac gcgccgacct cgccccctac cggagcggcg atctcgagat ctcgctcacg
     2461 tggccggaga cgagcgacta cgacatcttc gtcttcgacg cgcaggggac ggagatcgcg
     2521 cgttcagccg ccagcaacat cgacggcggc gacacccggt tcgagcgggc gctcgtcgag
     2581 ggcatcgccc actgtgccga cctctcgatc gcggtgcgca gctgggccgg ccgtccggat
     2641 cagacgctga gcctgcgcat cgccttcggc aaccccggac cgatgttcgc ctgcgagaag
     2701 gacgacccgc atccggcgtg cgaggggaag gaggagggcg agccgccggc acgccttccc
     2761 gatacgcgca cccgtctgta cctcggcggc gaccgcccgg ggcagctctc gatgcagggt
     2821 cattacgtcc acgacctcga ggtgaacggg atccgccccg tcggcggcga ggcggagctc
     2881 tcatcgcacc tggttccggt acgcccgacc ggcggacgtc ccaaccactt cacgaagctc
     2941 gtggccggga acccggagca gcacgagaac ccgttcatgg cgaactactc cctccagctg
     3001 aagcagccga cgaggatcac gggcgatgtc aaggcgctca tctgggtttc ctcccagacg
     3061 ttcgggcagg gtgggggagg cgggcccctg ttcgtggacc tctggcagga cgggaccgcg
     3121 gcgctggccg ggacacggat cgcacgcgtc gaggtcccgg gctcgaagat cggcgcggcg
     3181 cccaccccgg tccgcgtgac cttcagcggc gtcgacgcac tcgtcgagcg ggagctggtc
     3241 ctgcaggtct catccgagcc cgtcgcggga acgtcggggg gctcgcagga ccccgcagac
     3301 tcggagtgga cgatctacta cgacagcgtc cagttccccg cgcacctgac gctgaacctc
     3361 ccccaggcct gagcggccgc gttcggcgat tcggctagca tggcgccgat gttcctcgac
     3421 gtgacttccg acaacccctt cgcgagcaac tgctgggccg tctccgccga cggatccgac
     3481 gaggccgtcg tggtcgaccc gggtttcgag cccgaggtga tcgcgcggat gctcgagcgg
     3541 gccgggaagc ggctcgtggc ggtgctcgcg acgcacgggc acttcgatca catcggcgcg
     3601 gtcccgcagg tgtgcgcggc gggggtgccg gtcgtgatcc acgaggccga cgagctcgcg
     3661 ctctccgacc aggcggcctg gggctccggg caccccgtgc cgatggacct ccggccgaag
     3721 gacgtcatca cggtctcggg gggcgaccgc ctggagtacg cggggttctc cttcgaggtc
     3781 atgcacacgc cggggcacac ccccggaagc gcctgcttcg tgacggacgg gttcctcttc
     3841 agcggcgacc tcctcttccg cgggtcgatc ggccggcacg acttcccgaa ctcgagcgcc
     3901 cgcgacatgc gccgcagcct cggccggttc ctcgccctgc ccgaccgcct ccgcgtgtat
     3961 cccgggcacg ggccggagac gacggtgggc gacgagcggg tctcgaaccc atggctcgtg
     4021 gagctcgccg gggcccggga gtgaccgacc tcaacccgcc gcgcgggacc caggacctcc
     4081 ttccaccgcg gtccgacgcg gtcgaggcgc tcgcggacga agggcggcgg ctcgctcggc
     4141 gcttcggctt ccggcgcgtg gagacgccca ccttcgagca cacggagctc ttcgcgcgcg
     4201 gggtcggtgc gagcaccgac atcgtgacga aggagatgta cacgttcgag gacaagggag
     4261 ggcgctcgct caccctgcga ccggagggca cggcgccggt gatgcgggcc tacctcgcgc
     4321 acaagcagga gctcggggct ccgttcaagg cctactacgt cgtcccgatg ttccgccacg
     4381 agcggccgca ggccgggcgg atgcgccagc acacgcagct cggggtcgag gtcatgggaa
     4441 cggccgcgcc cgccgccgac gtcgaggtgg tcacgctcgg ccagcgtttc ctctcggggc
     4501 gcggcctgcg gcggttcgag ctgcgcctga actccatcgg ggacgaggtc tgccgcccgg
     4561 cgtaccggac cgcgctgatc gatttcctca aggagcgggt ggaccggctg tgccgggact
     4621 cgcgcgagcg gatgctccag aacccgcttc gcgtgctcga ctgcaaggtc ccctcgtgcc
     4681 gtgcggcgac cgaggatgcg ccgcggatca ccgaccatct ctgcgatgcc tgccgcgagc
     4741 atttcgacgc ggtccaggca gggctccgcg aggaggggat caccttcgtc ctcgaccacc
     4801 ggctcgtgcg cggcctcgac tactacacgc ggaccacgtt cgagttcgtg agcgaggtgc
     4861 tcggcccgac gcagtcgacg ctctgcggcg gcggccgcta cgacgggctc gccgagcttc
     4921 tgggtggcga gccgaccccc gggatcgggt tcggcctggg gctcgagcgc ctgttgatcg
     4981 cgatcgacga ggagggggtg cccctgcccg agccggagcc gctcgcggtg ttcgtcgtcg
     5041 ccctcgggga ggaggcggcg gccgaagcgc gggcgctcgt gcgcgagctc cgcgaggagg
     5101 gtctggccgc cgcgggctcg ctcgaggacc ggccgatgaa agcgcagatg aagatggccg
     5161 accgcgcggg cgcgcggtac gtcgcgatca tcggagagca ggagctcgcg gacggcgtcg
     5221 tcacgatgcg acggatggcc gatggcgagc aggagagcgt gaagcgctcg gaggtgcccg
     5281 gatggatctc ccgctgagga ccgggtactg cggcggcctg cgcgcggagg acgccgggcg
     5341 ggaggtcgcg ctgtgcggat gggtggcccg cagccgggac cacggcgggg tcgtcttcat
     5401 cgacctgcgc gaccgcgagg ggatcgcaca ggtcgtcttc catccgcagg acgcccccga
     5461 ggcgcacgcg gtggcagccg acctcaaggc cgagagcgtc gttcgggtcc tcggcgaggt
     5521 gcgcgagcgc ccggagggga cgcggaaccc gaacctgccg accggcgacg tggaggttgc
     5581 cgcgcgcgag gtcgaggtgc tgtcggtcgc cgagacgcca ccgttcccgg tcgaggacga
     5641 cgtgcaggcc gacgagcgcc tgcgcctgcg tcaccgctac ctcgacctcc gccgcccgga
     5701 gatgacgcat acgctgcgcc tccggcacgc ggctttctcg gcgatccacc gcttcatgga
     5761 cgagcacggg ttcgtcgagg tggagacgcc gatgctcaca cgcagcacgc ccgagggggc
     5821 tcgtgacttc ctcgtcccgt cgcggctcca ggaggggacc ttctacgcgc tcccgcagtc
     5881 cccgcagatc ttcaagcaga tccttatggt gtcgggcctc gaccggtact accaggtcgt
     5941 gcgctgcttc cgcgacgagg acctgcgcgc cgaccgccag ccggagttca cccagctcga
     6001 cgtcgagatg tcgttcgtga acgaggagga cgtgcacgcg gtcgtcgagg agatgctggc
     6061 cgccgtctgg cgcgaggtgc tcgatgtgga gatcgcgacg ccgttcccgc ggctgacgta
     6121 cgacgaggcg atgcggcgct acggcagcga taagcccgac acgcgctacg gcatggagct
     6181 ggcggacctc acggagacct tccgtggcac cggcttccgt gcgttcgcgt cggtgatcga
     6241 cggcgggggc gtggtgaagg gcttcgcggc gccgggggcg gcgtcgtggt cgcgcaagga
     6301 gctcgacgac ctggtggtcg aggcgcaggg ccggggggcg tcgggactcg tgtgggtcgc
     6361 gttcgcgggc gacgaggttc gctcaccggt ggcgaagttc ttgagcgagg gggagctggc
     6421 ggcgcttcgg accgagaccg gtgcggggga tggcgacctc gcgctcgtcg tcgccgatgc
     6481 ggaggggcgc gcgaacacgg tcctcgacgg gctgcggcgg ctgatggccg accgcctggg
     6541 gctcgtcccg gaggggcggt gggacttcct gtggatcacc gagccgcccg tcttcgagtg
     6601 gagcgaggag gacgagcgct gggtgagcgt gcaccatccg ttcacgtcgc ccgccaccga
     6661 cgacctctcg cccgagacag cgaaggcgcg cgcctacgac gtggtgctga acggctggga
     6721 gctcggcggc ggctcgatcc ggatccaccg cccggacgtc cagcgcgcgg tcttcgaggc
     6781 gctcgggatg tcgaacgagg aggccgagga gaagttcggg ttcctcctcg acgccttccg
     6841 ctacggtgtc ccgccgcacg gcgggatcgc cttcgggttg gatcggatga cgatgctgct
     6901 cgcgggccgg tcgaacatcc gtgaggtgat cgccttcccc aagacccagt ccggcaccga
     6961 cctcctgacg ggcgcccccg ccgaggtcgc cgacgaccag ctccgcgagc tcggcatccg
     7021 cctgcgctga gcctcggaac gccgctgcct gcacggacga gacgcgcccc ggctcgcccg
     7081 ccggtcgctc aggcggtcgc gagcgacgcc tctggaggag ccgcgtcctc ggaaggaccg
     7141 acccggtcgt tcgccggtcg gtgaggccat ccggccgatg gctccccggt ctgggcggtg
     7201 caggtctcca ccctctcgtg ccgccgcgga accgcccgtc ctcgccatgt acttcgcctc
     7261 cctcgctccc gataccgcat cccaacaccg gacgaaggga ggaagggatg gcggcatcga
     7321 agatcgtgaa gaggcgggtg gcgacatcgg cgctcgtcgt gctcgtgttc caggggatcc
     7381 tcgccgtcgg cgcgcaggca aagggcgtgg ccggaggccc gcgtggtggg cagcccgcgg
     7441 gagaggctcc cgtctccgag gtcgggcctc cggggccggg ccgacctgct tcgcccgagg
     7501 cgcctccgca gcccccggcg ccgccccaga gcgatgggca gccgaacggg aacgccggcc
     7561 cgggaccttc tcaccacgcg gaagagccga gcggtcccgt gggtcacgga gcgtctcacc
     7621 acgcggaaga gccgaagggg cccggcggtc ccggagcgtc ttcgggcgac gcgggcgatc
     7681 ggcggccgga tctctctgcg gggaccggcc cgtctgcggc agccgaccac gggccgggat
     7741 cccaccaggc cgacgaccgt cccgggcatg cgcccgacca cgcgcgctcc tcggagcggc
     7801 cgggcccacc ggaccacgcg ggagcacccg aacacgccgg cgcgcccggg cagggcgctc
     7861 cgcacgcgac gggtccggcc ggcggcgagt ggccgggtgg ggctcgaccg ccgatcctct
     7921 cgcggccgca gcacggctgc tgggcctcct cgatgacccc gttcatgtgc cggatctccg
     7981 agcaggagga ggacccgacg cagccggctc cgaaggagca gacggatcct gggctcgacg
     8041 aggagctgat ccagccgggc ccggacgagg agcccgtggt cgacccgtcg cccgagcgtg
     8101 acggagggtc gacgacgccg gtgcgcccgg ggccgggcgt ccccgccggc cgctcctcga
     8161 cgcccgcgat cgcaccgggg gcggcacccg cggcggcatc cgcccacggg cccgacacgg
     8221 gtggatccac ggggcgcgac acgtcgagcc gcgactacca cccggcgatc gacacgccgc
     8281 gcgggagcct cccggtgccg ccggtcggag cttcggggat gctgggggca gccttcggcc
     8341 tcctgatgct ggcaggtgcc ggcggcggcc tcggggcggc cctgctgcgc ctgcgcgcgc
     8401 ggctggcgtg agggttggac ggccacgccc ctatgctggg ggcgtggccg tcccactcgc
     8461 cgctcggatg cgtccccgga ccttcgatga gttcgtcggc cagcggcacc tgatcggccc
     8521 ggggaaggcc ctgacggcac tggtggacgc ggggcatctc ccttcgctga tcctgtgggg
     8581 cccggccggc acggggaaga c
//
LOCUS       DATLGG010000075         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_98645, whole genome shotgun sequence.
ACCESSION   DATLGG010000075 DATLGG010000000
VERSION     DATLGG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98645"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM638_02020"
     CDS             complement(<1..230)
                     /locus_tag="VM638_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HVM11241.1"
                     /translation="MATRMKWYADRVRRYTHTGPQAAHRIASVQAAGIAAQAVRSKGT
                     GALARDVSRPRFVGFLRAVVGSTLPYARIQNFG"
     gene            complement(266..922)
                     /locus_tag="VM638_02025"
     CDS             complement(266..922)
                     /locus_tag="VM638_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11242.1"
                     /translation="MPADYFSYAQFRSFANDPLPAEGEPKYLNADIERAQIEVVEHLE
                     QWAGSAWPTVVEQASPDEIGWTTVPRRRREQVLLAADGRVRMAYAPIISIEAVEVGGE
                     EVEFETQTFRGELASFTLSVPSLPRDRLVVLDFTYGHTSTPWAIRRPAMMATKSLLDG
                     EEGRGGKIPSRVSRYDTEGTTFLLERDEPKLPWPWDPDASAAVHAWWGARRPRSVAFV
                     "
     gene            complement(922..1935)
                     /locus_tag="VM638_02030"
     CDS             complement(922..1935)
                     /locus_tag="VM638_02030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM11243.1"
                     /translation="MKVRFVASDEAGGGRLRSFWPAAELRRRGHDAEASTEWPEDADV
                     VVVHRPLDPETLEQVRLYRAAGVRVLVDEDDDLRSVPPENRNFHRWFTPERREVHERA
                     IAEADGLIVSTDRLAEVYGPLAREVHVCRNHLPAFVGRLRPMRSRHDQAFRVGWAGVV
                     KTHRQDLEWLAQAGARPFDGAILSQVGDPRAGRCLPHFGIETFPFQETPEGLYRLMAR
                     ADVGIVPLLPCAFNVAKSWLKALEYMSVGVPVVATDLPEQRRLIRHGTDGFLASDPFD
                     FAEHVQTLRRDPDLRQEMALRARRRAAELAIEVHGWAWEDALGLTRALDPSVRVPLMA
                     GGA"
     gene            complement(1932..2318)
                     /locus_tag="VM638_02035"
     CDS             complement(1932..2318)
                     /locus_tag="VM638_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11244.1"
                     /translation="MPLVRGGALRGPSPRAAGQTVVRGAPPAPGPKPSAEETPYRSLF
                     YVSKRRSADGNAYRKGEPIPEPEARRQGQRVGEPQLRFRVPKLICRRCEGRGKAYMKG
                     HKPDCGCPGCRPCKECGGTGRVVGAR"
BASE COUNT          354 a          889 c          864 g          335 t
ORIGIN      
        1 ccgaagttct ggatccgggc gtagggaagc gtggatccga ccacggctcg gaggaagccg
       61 acgaagcggg gccgcgaaac gtcgcgcgcg agcgcgcccg tacccttcga gcggaccgcc
      121 tgggcggcga tgccggcggc ctgaacggac gcgatccggt gcgccgcctg cggcccggtg
      181 tgcgtatagc ggcgcacccg gtccgcgtac cacttcatcc tggtcgccat ctcagttccc
      241 gagtctggcg gggtggcggg acgcctcaga cgaaggcgac cgagcgtggg cgacgtgctc
      301 cccaccaggc atgaaccgcc gcggaggcgt cggggtccca cggccacggg agcttcggct
      361 cgtcccgttc gagcaggaag gtcgtgccct ctgtgtcgta gcggctgacg cgggatggga
      421 tcttcccgcc gcgaccttcc tcgccgtcga gcaggctctt ggtcgccatc atcgcggggc
      481 ggcggatcgc ccagggcgtg gacgtgtgcc cgtaggtgaa gtcgagcacg accagccggt
      541 cccgcggcag gctcggcacc gacagcgtga acgaggccag ctcaccccgg aaggtctgcg
      601 tctcgaactc gacctcctca ccgccgacct cgacggcctc gatgctgatg atcggggcgt
      661 acgccatccg gacccgcccg tcggctgcga gcagcacctg ctcgcgccgg cgccgaggaa
      721 ccgtggtcca cccgatctcg tcggggctcg cctgctcgac gaccgtcggc cacgccgatc
      781 ctgcccactg ctcgaggtgc tcgacgacct cgatctgggc gcgctcgatg tccgcgttca
      841 ggtacttcgg ctcgccctcg gccgggagcg gatcgttcgc gaacgatcgg aactgcgcgt
      901 agctgaagta gtcggccggc atcaggcacc ccccgccatc agggggacac ggacgctggg
      961 gtcgagtgca cgggtcagcc ccagcgcgtc ctcccaagcc caaccgtgga cctcgatcgc
     1021 gagctccgcc gcacgccgcc gcgcccgcag cgccatctcc tgccgaaggt cggggtcgcg
     1081 gcggagcgtc tggacatgct cggcgaagtc gaaggggtcg gaggcgagga agccgtcggt
     1141 gccgtggcgg atgagccgcc gctgctcggg aaggtcggtc gcgacgacag ggactccgac
     1201 gctcatgtac tcgagggcct tcagccagga cttcgccacg ttgaacgcgc acgggagcag
     1261 cggcacgatc ccgacgtccg cccgcgccat cagccggtac agcccctccg gcgtctcctg
     1321 gaaggggaag gtctcgatcc cgaagtgggg caggcaacgc cccgctcggg ggtcccccac
     1381 ctgggagagg atcgcgccgt cgaacggacg ggctccggct tgcgcgagcc actcgagatc
     1441 ctggcggtgc gtcttcacga cccccgccca tccgacacgg aatgcctggt cgtgccgcga
     1501 gcgcatcggg cgcagccgcc cgacgaacgc gggaaggtgg ttccggcaga cgtggacctc
     1561 gcgagccagc ggcccgtaca cctcagcgag ccgatcggtc gagacgatca ggccatccgc
     1621 ctcggcgatc gctcgctcgt ggacctcccg gcgctcgggg gtgaaccacc ggtggaagtt
     1681 ccggttctcg ggagggacgc tgcgcaggtc gtcatcctcg tcgacgagca cgcgcacccc
     1741 tgctgctcgg tagaggcgca cctgctcgag ggtctcgggg tccagtggtc ggtgcacgac
     1801 gacgacgtcc gcgtcctccg gccactcggt ggaggcctcg gcgtcatggc cgcggcgacg
     1861 aagctcagcg gccggccaga acgagcgaag ccggccccct ccggcctcgt ccgaggcgac
     1921 gaagcgaacc ttcaccgcgc ccccacgacg cgtccggtgc cgccgcactc cttgcacggc
     1981 cggcacccgg ggcagccgca gtccggcttg tggcccttca tgtaggcctt cccgcgcccc
     2041 tcgcagcgcc ggcagatcag cttcgggacc cggaaccgca gctggggctc gccgacgcgc
     2101 tggccctgac gacgcgcctc cggctcgggg atcggctccc ccttccggta cgcgttgccg
     2161 tcggcggagc gacgcttgga gacgtagaac agcgatcggt acggcgtctc ctcggcgctc
     2221 ggcttcggac ccggtgcggg aggagccccc cggacgaccg tctggcctgc ggcccggggg
     2281 gagggacccc gaagagcccc tccccggacc agcggcatca ggccaggtcg accgcgacga
     2341 acgccttcgg ccgcacgacc gtgaaggcgg cccgcatctc cgcgagcagc cgcacgatgt
     2401 tgcggatgaa gtcgtcgccg gcggtcccga ccgagatggt cg
//
LOCUS       DATLGG010000076         4151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_84145, whole genome shotgun sequence.
ACCESSION   DATLGG010000076 DATLGG010000000
VERSION     DATLGG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84145"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..480)
                     /locus_tag="VM638_02040"
     CDS             complement(226..480)
                     /locus_tag="VM638_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11245.1"
                     /translation="MDENIYRVGIERSPAGWTVHIVDPEGQGVFERPCADEVEARTYA
                     STVRQHIDWLSPERFRAYYRLPDQGVPQRATPEQALPEGR"
     gene            575..2197
                     /locus_tag="VM638_02045"
     CDS             575..2197
                     /locus_tag="VM638_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HVM11246.1"
                     /translation="MGHRTRRIALSASLALLAGGLLAPAAAAPAAPIQIQLLSVSDWH
                     AQLDPVSVSGVGDVGGAAALSSYFRAERAANPNTLTLTAGDAYGASPPLSGFFDEEPA
                     VKAMNLMGFDADTFGNHNFDRGVGHLQRMIDLAEFPYVSANLRNVEGNLTGVRPYEIF
                     DVSGIKVAVIGLTNPEAPELVTPGSLGTIEITDPVQAANRARAQARREGAKVFVIITH
                     MGVTGFDAAGQPVGPLVDLANAVGGFDLIVGDHTDIQYRGVINDQLVVENRSKGLTYA
                     RTVLSVNPTSGRVIDASSEFVTPRAASIAPDPAVVQMLRPYRDALVAAMDQPAGVATG
                     VFPRAGNIERLREVALGNLITDAMRETYGTQIAVQNGGGIRAPLPSSYLPADTSLRRT
                     TPGYAAGPPYDLVVGDIYTVLPFGNAVLTRTVTGLQLWSMLEHSVAPIPSSNGRFLQV
                     SGFRFTYDAGLPPGSRVLSVTLDDGTSILPDATTYTLATNNFMNAGGDGYTMLADGQG
                     VTRDLDANVLLEYIRSKGTITPTIEGRITRLN"
     gene            complement(2181..3089)
                     /locus_tag="VM638_02050"
     CDS             complement(2181..3089)
                     /locus_tag="VM638_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVM11247.1"
                     /translation="MNPVRVGTSSWTDRTLLESGWYPDDAKTPEDRLRFYAERFDVVE
                     VDSTYYSLPSERNSALWVERTPPRFVFHVKAFAAMTGHPVTVRSLPKAVKELLPEGKD
                     RLYPKDLPDDAADLCWDMFRSALMPLHSAGKLGVVMFQYPQWFMPSRRSKDDILRARE
                     RLPDYRIAVELRQAAWFKDEEMTRRTLDFFRSEEIPFVCVDMPQGFPSSIPPIAEATN
                     DRIALVRFHGRREETWGKRGVPVLERFRYDYPKEELEQWVPKVAALQEQARETHALFN
                     NCYRDSAVRNAATFAGMVEEQLSSAG"
     gene            complement(3086..3376)
                     /locus_tag="VM638_02055"
     CDS             complement(3086..3376)
                     /locus_tag="VM638_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11248.1"
                     /translation="MRIKGTCARCGREFLAREAIDNGGHCPSCGQPFNAHYTAVLAST
                     LREAEVAGSVIEDALNRLVGMEGALELGEESILEPLRQPIRATRRGKIRPRR"
     gene            complement(3373..3780)
                     /locus_tag="VM638_02060"
     CDS             complement(3373..3780)
                     /locus_tag="VM638_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11249.1"
                     /translation="MKALLISRDEDVRTQLGVALRAVERRTKAPWEFLEAADGIEGLR
                     LAWKHLPDVVVADEITSRAGAFAVAKDLTGAVNPFPGAIVIVLARPEDAWLARWSGAD
                     AWLNRPLDPFALADIVTDLVAPAPPAAVGGGGA"
     gene            complement(3783..3965)
                     /locus_tag="VM638_02065"
     CDS             complement(3783..3965)
                     /locus_tag="VM638_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11250.1"
                     /translation="MRAPGGTVSEKQTKPSEDDEKQRSCQVCGVKLSAYNPNPTCWQH
                     TIGHPWRGPSAKPRYD"
BASE COUNT          621 a         1461 c         1439 g          630 t
ORIGIN      
        1 accaccggat ggtgcacgag aggggtgggt tctccctccg cttggaggag gggaggccgg
       61 tgttccgcag gcccgacggg tcggtgctgg aggaggcgct ggcggagcgg gggccgccct
      121 agctgagggc ccagatcacc gcgacggcga tcgcggcgat cgcggtgaag aacatcacga
      181 ggtcggcggt cttcgggcta cgaccctgca tggggttgaa gcccatcagc gaccctccgg
      241 cagggcctgc tccggcgtag cgcgctgcgg cacgccctgg tcgggcaggc ggtaataggc
      301 acggaagcgt tcgggcgaga gccagtcgat gtgctggcgc acggtcgagg cgtaggtgcg
      361 ggcttcgacc tcgtcggcgc atggccgctc gaacacgccc tgcccttcgg ggtcaacgat
      421 gtgcacggtc cagccggcgg gcgatcgttc gatgccgacg cggtagatgt tctcgtccac
      481 gcctggatga tacgtgcccg ggaaccattg acacgcacaa cggtcatgcg ggaagattcg
      541 ccgtcacgtc cgaaatcaga cagggaggga catcatgggg caccgcacgc gccgcatcgc
      601 gctgagcgct tcgctcgcac tgctcgccgg gggtctgctc gcgccggcag cggccgctcc
      661 ggcggctccg atccagatcc agctcctgtc ggtctcggac tggcatgcac agctcgaccc
      721 ggtgagcgtc tccggggtcg gcgacgtcgg cggcgcggcg gcgctctcct cgtacttccg
      781 cgccgagcgt gccgcgaacc cgaacacgct gacgctgacg gcgggcgacg cctacggcgc
      841 ctcccccccg ctctccgggt tcttcgatga ggagccggcg gtcaaggcga tgaacctcat
      901 gggcttcgac gcggacacgt tcggcaacca caacttcgac cgcggggtgg gacacctcca
      961 gcggatgatc gacctcgcgg agttccccta cgtctcggcg aacctgcgga acgtcgaggg
     1021 gaacctcacc ggggtgaggc cctacgagat cttcgacgtg agcgggatca aggtcgccgt
     1081 gatcgggctg acgaacccgg aggccccgga gctcgtcacc ccaggcagcc tcggcacgat
     1141 cgagatcacc gacccggtcc aggcggcgaa ccgcgctcgg gcgcaggccc gccgagaggg
     1201 cgcgaaggtg ttcgtgatca tcacccacat gggcgtcacc ggtttcgacg ccgcgggtca
     1261 gcccgtcggg ccgctcgtcg acctggcgaa cgccgtcggt gggttcgacc tgatcgtcgg
     1321 cgaccacacc gacatccagt accgcggcgt catcaacgac cagctcgtcg tggagaaccg
     1381 tagcaagggt ctgacgtacg cgcggaccgt cctgtcggtg aaccccacca gtggccgggt
     1441 gatcgacgcg tccagcgagt tcgtcacccc ccgggcggcg tccatcgctc ccgaccccgc
     1501 tgtcgtgcag atgctgcggc cctatcgtga cgcgctggtg gccgcgatgg atcagcccgc
     1561 cggcgtggcg accggcgtct tcccgcgtgc ggggaacatc gagcgcctgc gggaggtcgc
     1621 cctcggcaat ctcatcaccg acgccatgag ggagacctac gggacacaga tcgcggtgca
     1681 gaacggcggt ggcatccgcg cgccgctccc ctcgagctac ctgccggcgg acacgtccct
     1741 ccgccggacg acgccgggct acgccgcggg tccgccctac gacctcgtgg tcggcgacat
     1801 ctacacggtg cttccgttcg gaaatgccgt cctgacgcgg acggtcacgg gcctgcagct
     1861 ctggtcgatg ctggagcaca gcgtggcccc gatcccctcc tccaacggac gcttcctcca
     1921 ggtatccggc ttccgcttca cgtacgacgc agggctgccg ccagggagcc gcgtcctctc
     1981 ggtcacgctc gacgacggaa catcgatcct gccggacgcc acgacctaca cgctcgcgac
     2041 gaacaacttc atgaacgccg gcggggacgg ctacacgatg ctcgcggacg gccagggggt
     2101 cacgcgggac ctcgacgcga acgtgctcct cgagtacatc cgctcgaagg gcacgatcac
     2161 gccgacgatc gaggggcgga tcacccggct gaactgagct gctcctccac catcccggcg
     2221 aaggtggcgg cgttgcggac cgcgctgtcg cggtagcagt tgttgaagag cgcgtgggtc
     2281 tcccgcgcct gctcctgcag cgccgccacc ttcggcaccc actgctcgag ctcctccttc
     2341 gggtagtcgt agcggaagcg ttcgaggacc ggtacgcccc gcttgcccca ggtctcctcg
     2401 cgccggccgt ggaagcggac gagcgcgatc cggtcgttcg tcgcctcggc gatcggcggg
     2461 atgctcgagg ggaacccctg cggcatgtcg acgcagacga acgggatctc ctcgctgcgg
     2521 aagaagtcga gcgtccgccg ggtcatctcc tcgtccttga accacgccgc ctgccggagc
     2581 tcgaccgcga tccggtagtc cggaaggcgc tcgcgcgcgc gcaggatgtc atccttcgag
     2641 cggcgcgacg gcatgaacca ctgcgggtac tggaacatca cgacgccgag cttcccggcc
     2701 gagtgcaggg gcatgagcgc cgagcggaac atgtcccagc acagatcggc cgcatcatcc
     2761 gggaggtcct tcgggtagag gcggtccttc ccctccggca agagctcctt caccgccttc
     2821 ggcagcgacc ggaccgtgac cggatgcccc gtcatcgccg cgaacgcctt gacgtggaag
     2881 acgaacctcg gtggcgtgcg ctcgacccac agggcggagt tccgctcgct cgggagcgag
     2941 tagtaggtgg agtccacctc gacgacgtcg aaccgctcgg cgtagaagcg gagccgatcc
     3001 tcgggggtct tcgcgtcgtc ggggtaccag ccggactcga ggagggtgcg gtcggtccac
     3061 gaggaggtcc cgacccggac cgggttcacc gccgcgggcg gatcttccct ctgcgagtgg
     3121 cgcggatcgg ctgccggagc ggctcgagga tgctctcctc gcccagctcg agcgcgcctt
     3181 ccatcccgac gagccggttc agcgcgtcct cgatcaccga gccggccacc tccgcctcgc
     3241 ggagggtgct cgccagcacc gccgtgtaat gcgcgttgaa cggctggccg cacgacgggc
     3301 agtgcccacc gttgtcgatc gcttcgcgcg cgaggaactc gcgtccgcac cgcgcgcagg
     3361 ttcccttgat cctcatgcgc cgcctcctcc caccgcggcc ggcggtgcgg gagccaccag
     3421 gtcggtcacg atatccgcga gcgcgaacgg gtcgagcggg cggttcagcc acgcgtccgc
     3481 ccccgaccag cgggcgagcc aggcgtcctc gggccgcgcg agcacgatca cgatcgcgcc
     3541 agggaacggg ttcacggcgc cggtcaggtc cttcgccacc gcgaaggctc cggcccgcga
     3601 ggtgatctcg tccgccacga cgacgtccgg aaggtgcttc caggccaggc ggaggccctc
     3661 gatgccgtcg gcagcctcga ggaactccca cggagccttc gtccgccgct cgacggcgcg
     3721 gagagcgacg cccagctggg tgcggacgtc ctcgtcgcgg gagatcagga gggccttcac
     3781 gctcagtcgt accgcggctt cgccgacggg ccgcgccagg ggtggccgat cgtgtgctgc
     3841 cagcaggtcg ggttcgggtt gtaggccgac agcttcacgc cgcagacctg gcacgatcgc
     3901 tgcttctcgt cgtcctcgga aggcttcgtc tgcttctcgc tcacggttcc cccaggggct
     3961 ctcacctggg gattctacca tcgagaccgc tcagccgacc tgccaggtgt cccccgaatg
     4021 gaggagcttg gcgaggtctc cctcgccgag ccgctcggtg gcggccgcga gctggcgctc
     4081 catcgcctcg ccgtagaccg ggcgcgcgat gtcgcggaag atcccgatgg gggtggggcc
     4141 ggtcgggcgg t
//
LOCUS       DATLGG010000077        10810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_37543, whole genome shotgun sequence.
ACCESSION   DATLGG010000077 DATLGG010000000
VERSION     DATLGG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37543"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1166
                     /gene="dapA"
                     /locus_tag="VM638_02070"
     CDS             282..1166
                     /gene="dapA"
                     /locus_tag="VM638_02070"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HVM11251.1"
                     /translation="MAGRFGTLITAMVTPFTRDGSVDLDRAQEVAAWLLEHGSDGLVV
                     AGSTGEAATMSDDEKIDLWRAVVEAVGDRGTVIAGTGTYDTAHTIHLTESATSAGVDA
                     ILVVTPYYNKPPQAGLIEHFTAAARATELPVMLYNIPGRTGTRIEHDTLLRLAEVENI
                     VAVKDSTGDFDGAAKLMAAAPSGFEVYSGDDWATFGFATLGSPGVVSVVSHVVGERMR
                     RIFELIEAGDLPGARKVHTELLPVFDVMFMTSNPIPVKAAMALIGQPVGDARLPLVPA
                     TDAQVAAIRKVLEDVGAL"
     gene            1150..2805
                     /locus_tag="VM638_02075"
     CDS             1150..2805
                     /locus_tag="VM638_02075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HVM11252.1"
                     /translation="MSARCRLVFLGGLGEVGRNIACLEIDDDVLVLDAGLSFPTEDMP
                     GVDLVLPDYQYLRERQDRIAGVVLTHGHEDHMGALPYILKEIALEIWAPPLALGLLRP
                     KLEEHGVTERATLHELRAGDEARVGPFTVRAIRVTHSIPEGLALAIDTPFGTVLHSGD
                     FRLDPTPVDGRATDLQAFGEEGRRGVHVFLSDSTNAEDPGIIPSERTVGPELLRIFEG
                     ASRRVVAACFSSHIHRIQQICDAALAVGRRIAFFGRSMHHAVAQTRELGHLHVPEDAI
                     LDIEEIAKLPPEQVAVICTGSQGEPLSALSLMAAREHKWVKLEEGDTVVIAASLIPGN
                     EVAIHRTIDGLFRTGADVFHTGIAHVHVSGHAAAEELKFMLGLVTPRWFIPIHGERRH
                     LSHHAKLAREVGIPDERILICEDGDVVEIGEKVEVVDRVEAGMVLVDGLGIGDVGAVV
                     LRDRRKLAGDGVVIVVVTADAQSGELLAGPDIVTRGFVHDESSAEMLEEARRRTRESL
                     EAVAREEVGDPAILKQHVRRTLGRYFFEVTKRKPVILPVIMEV"
     gene            2806..5034
                     /locus_tag="VM638_02080"
     CDS             2806..5034
                     /locus_tag="VM638_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HVM11253.1"
                     /translation="MAKTKKRKKATKRGSGVATAGRRVAEHLEPRHQDAIGIGLIVLG
                     GLSALGIWLDAAGPVGRFLDMLARGAVGVVGYAVPVLLLWWGLVLVRGTAQEERGRML
                     VGLVFALAGGLGLVSLFRGNPSPAAGLSGFGDAGGILGAATAWPLSRLASAYGAALVC
                     LGLFVLGILVFTATPLSAVWEWFRREQAPAEAAAAEDPMPEAEPAPRKRKRRRLRDLT
                     DEEVAEATRWEEMGEEVPADAPAEPEPAPPPPPPVPSGRGDYKLPPLKLLRTGPGTSA
                     ASRDEKETMATLERTLRQFGVDATVAGFSRGPTVTLYEVGVAAGTKVNKVLSLGDDIA
                     YALATPDVRIIAPIPGKSAIGIEVPNRVRDFVMLGDVLGAKETKEERHPLAVGLGKDV
                     HGNAVMCRLHEMPHLLIAGTTGAGKSSLINAFVTSILMRATPDDVKLMLVDPKRVELT
                     HFVDLPHLISPVVVNPKRAAEALGWVVREMEMRYEMLATVGVRDIIGYNGGLAEGTLR
                     IPPGRDAEFERLPYIVVVIDELADLMMVAPRDVEDAICRIAQMARAVGIHLVVATQRP
                     SVDVVTGLIKANIASRIAFMTASQADSRVILDVGGAEKLVGHGDMLFQPTGASRAARI
                     QGAWVTEKEIEQIADWCRAQQEPVYVQGIEGVGRPPSEEDAEDGDDDLLTQAMELVIR
                     SQLGSTSMLQRKLKVGFARAGRIMDLLEERGVVGPSVGSKPRDVLITWDEWLESREAR
                     AG"
     gene            5048..6121
                     /locus_tag="VM638_02085"
     CDS             5048..6121
                     /locus_tag="VM638_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVM11254.1"
                     /translation="MSREMPARAVVVAADPGAREAMRRAIDEVPNVEVVGEADDPKDA
                     AILAARAHPDLVLIALSPADENAAWGLPSLLGVAPGARIVLTLLEEWSNERPQQGYDL
                     REIIAVLREARAADDRPEGHRRREATLSMLAHDILTPTTAVVGVADLLLRYWDRFDDQ
                     QRREGVANIAEVGRDLASLVKGVVRAAAADAGSLVGDREPVELGELLEQVVRGMQAAA
                     QDHRIELETAAELPTVWIDRTRIQEAVLNFLSNAVKYSPAGTTVRVGAEAGEREVRVT
                     VTDEGPGIDPKDHGKLFRKFSRLHPEETPGHGLGLYLVKAIVEAHGGRVWVDGSPGAG
                     STFGLALPATGRTAGTGRSGRRD"
     gene            6152..6613
                     /locus_tag="VM638_02090"
     CDS             6152..6613
                     /locus_tag="VM638_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVM11255.1"
                     /translation="MRSPRHARRLPVLRRPLLGLLAALAAVSVLSGFTATAAPRPAPE
                     PDPVPSWHEETAPVSFAGVASWYGEWFRGKPVACGGEPYDPDALTAAHRWLPCGTKLR
                     LSHASREVVVTITDRGPFVEGRVLDLTPAAFAALADPDRGLIGVRATLVSP"
     gene            6697..7428
                     /locus_tag="VM638_02095"
     CDS             6697..7428
                     /locus_tag="VM638_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM11256.1"
                     /translation="MSANGTGIGRALRTARLRRGKSLEEASRETRVKTEYLQALEVEA
                     FDSLLGDVYVRGFLRSYARYLGLNPEKVVAFYQRAYRRDTPAPAPLHRAPGVVREPLL
                     EPKEHKHRTAWILAAGAAVIVLISAAAVGLLSRETPAPTPAAPAQAAAVPVAPEKVQV
                     DIVAEEAVGASVIVDGRPELGFNGLLEAGEAHSFEGERSIEVHLDSGYRAEMVVNGES
                     LGRPGMPNRPYERTFTAEDFRGKKG"
     gene            7449..7970
                     /gene="pgsA"
                     /locus_tag="VM638_02100"
     CDS             7449..7970
                     /gene="pgsA"
                     /locus_tag="VM638_02100"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HVM11257.1"
                     /translation="MGWPNLVSVVRIALAPLLVGLILVGDRRASLIAAAVFIAGAATD
                     RLDGYLARRFASATRTGAWLDPLADKLFTLSPVVALAAVRRFPLWAAAIFLVREAAVS
                     LLRARLGARGHSMPATPLAKWKTATQMLAILLYILPLDPAADPLRLGVLAIAVALTLW
                     SGAEYFVRRAPAP"
     gene            7967..9202
                     /locus_tag="VM638_02105"
     CDS             7967..9202
                     /locus_tag="VM638_02105"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HVM11258.1"
                     /translation="MRAEVIGIGTEILLGEIANTNARWISERLAEVGVDVLHHQTVGD
                     NLERIADAFRLALSRADVVIATGGLGPTGDDITREGLAAALGVELRRERAIEDHLRAW
                     FAGKRRTMPESNLRQADLPEGARWIRPELGTAPGLILERQGRRVYVVPGVPAEMREMM
                     EREILPELRRLSGATIRSKVVKASGLAEAVVGERLAELFESSTNPTVAFLSSGGEVRV
                     RLTAKAETEEEAERLIHPVAEKVRGLLGDAVFGEDEERLEEVVGRLLAERELHLACAE
                     SLTGGLLAARLVSVPDASKHFSGAAVVYAPDAKRDVLGVSQATIDGPGTVSEECAIEL
                     ARGARKVFKADVGLSLTGVAGPEPLEGKEPGTVWVGLSTEATETARTFVSPGDRDMVR
                     RAATQAALDLLRRHLLRQG"
     gene            9199..9768
                     /gene="thpR"
                     /locus_tag="VM638_02110"
     CDS             9199..9768
                     /gene="thpR"
                     /locus_tag="VM638_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HVM11259.1"
                     /translation="MTEGKALRLFVCVDLSDEAAEILGRVLVPWQERLPRARWVARRK
                     WHVTVRFLGRTQPDLLDFVEEACRGAAAEVEPFEVSLAGLGVFPGRTRARVLWAGVDD
                     PRGALATLAAGLDRRLAPEFEPETRPFTPHMTVARFNPPEPFREHADALDRTELETAP
                     WEIDRLLLYRSQLSPKGSEYELLQSFPVG"
     gene            complement(9773..10165)
                     /locus_tag="VM638_02115"
     CDS             complement(9773..10165)
                     /locus_tag="VM638_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HVM11260.1"
                     /translation="MSEDWVMLRVRMGSEDAHYGGNLVAGATMLRLFGDVVTELAIRH
                     DGDEGLLVGYEKVEFKAPVYAGDFIEARGRIVRVGRTSRTCEFEAWKVIAAKPDLGDE
                     AAELLDEPILVGRATGTTVVPLDRQKNR"
     gene            complement(10162..>10810)
                     /locus_tag="VM638_02120"
     CDS             complement(10162..>10810)
                     /locus_tag="VM638_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVM11261.1"
                     /translation="LDAPDVGGGPWTEHAAKRLLREIGIPVTREEEVREVEEAVEAAQ
                     RIGFPVVAKAAGIAHKTEVGGVRLGLATEQAVREAAAEVLRLGAGLLVSEEVRGRLEL
                     LVGAFRDAQFGVCGLLGLGGIWTEALGQAVVLPGPGSEDVVRRALEDAPWGRLLLEDV
                     RGVPLPLGRVTDVLFRLIDLVEGMPVEAIEINPLVVREDGVVAVDALLVPREGAR"
BASE COUNT         1505 a         3899 c         3898 g         1508 t
ORIGIN      
        1 ccgcaccggg ctaggatagc gggcaggatg gcagccgaac aacgcggcga gaggcgcctc
       61 gcgtggcccg ggaaccacgg gaaccaccag agtcaccctc accacgcgtt caacatgtga
      121 ctgttgttgc tcctggtaag aggtgttagc atagccggca gcgtgggggc ggcccggccg
      181 ggcaatgcgg gtctcccgac tggggcccgg ccggtccccg cgcaggttcc agccggcccg
      241 gacgcgcccc cgcctcgggg cggtggcggc aaggagggga gatggccggt cgattcggaa
      301 ccctgatcac cgcgatggtc acgccgttca cgcgggacgg ctccgtggac ctcgatcgcg
      361 ctcaagaggt ggccgcctgg ctgctcgagc acggcagcga tggcctcgtc gtcgccggct
      421 cgaccggcga ggccgccacg atgtccgacg acgagaagat cgacctctgg cgggcggtgg
      481 tcgaggcggt cggtgaccgc ggcaccgtga tcgccgggac cggcacctac gacacggccc
      541 atacgatcca cctgaccgag tcggcgacct cggccggggt cgacgccatc ctcgtcgtca
      601 cgccctacta caacaagccg ccgcaggcgg gtctgatcga gcacttcacg gccgccgccc
      661 gggccacgga gcttccggtg atgctctaca acatccccgg aaggaccggc acgaggatcg
      721 agcacgacac gctcctgcgc ttggccgagg tcgagaacat cgtcgcggtg aaggactcga
      781 ccggtgactt cgacggggcc gcgaagctca tggccgcggc gccctccggt ttcgaggtct
      841 actcggggga cgactgggcg accttcggct tcgcaaccct cggctcgccc ggggtggtct
      901 cggtcgtctc gcacgtcgtc ggcgaacgga tgcgcaggat cttcgagctg atcgaggccg
      961 gcgacctgcc cggcgcgcgg aaggtccaca cggagctcct gccggtcttc gacgtgatgt
     1021 tcatgacctc gaacccgatc ccggtcaagg ccgcgatggc gctcatcggc cagccggtgg
     1081 gggatgcacg tctgccgctg gtgccggcga cggacgcaca ggtcgcggcg atcaggaagg
     1141 tgctggagga tgtcggcgcg ctgtaggctc gtcttcctcg ggggcctcgg cgaggtcggg
     1201 cgcaacatcg cctgcctcga gatcgacgac gacgtcctgg tcctcgacgc cggcctctcg
     1261 ttcccgacgg aggacatgcc gggggtcgac ctcgtcctcc ccgactacca gtacctccgc
     1321 gagcggcaag accgcatcgc cggcgtcgtc ctcacccacg gacacgagga ccacatgggg
     1381 gccctgccct acatcctcaa ggagatcgcc ctcgagatct gggcgccccc cctcgccctc
     1441 gggctgctgc ggccgaagct cgaagagcac ggtgtgaccg agcgggccac cctgcacgag
     1501 ctccgcgccg gggacgaagc gcgggtcggt cccttcaccg tccgggcgat ccgcgtcacg
     1561 cactcgatcc ccgaagggct ggcgctcgcc atcgacacgc cgttcgggac ggtcctgcac
     1621 tcgggggact tccggctcga ccccacgccg gtcgacggcc gggcgaccga cctccaggcg
     1681 ttcggcgaag agggccggcg cggcgtccac gtcttcctct ccgactcgac gaacgccgag
     1741 gaccccggga tcatcccgag cgagcggacc gtcggcccgg agctcctgcg gatcttcgag
     1801 ggggcgtccc ggcgggtcgt cgccgcatgc ttctccagcc acatccaccg gatccagcag
     1861 atctgcgacg ccgccctcgc tgtcggccgc aggatcgcgt tcttcgggcg cagcatgcac
     1921 cacgcggtcg cccagacgcg ggagctcggc cacctccacg ttccggagga cgcgatcctc
     1981 gacatcgaag agatcgcgaa gctcccaccc gagcaggtcg cggtgatctg cacgggcagc
     2041 cagggggagc cgctgtcggc gctgtcgctg atggccgctc gcgagcacaa gtgggtgaag
     2101 ctcgaggagg gcgacaccgt ggtgatcgcc gcctcgctga tccccggcaa cgaggtcgcg
     2161 atccaccgga cgatcgacgg cctgttccgc acgggcgccg acgtcttcca caccggcatc
     2221 gcgcacgtcc acgtctccgg gcacgccgcg gccgaggagc tcaagttcat gctcgggctc
     2281 gtcacccccc ggtggttcat cccgatccac ggggagcgac gccacctgtc gcaccacgcg
     2341 aagctcgcgc gcgaggtcgg catcccggac gagcggatcc tgatctgcga agacggtgac
     2401 gtcgtcgaga tcggggagaa ggtcgaggtc gtcgaccgcg tcgaggcggg gatggtgctc
     2461 gtcgacggtc tcgggatcgg tgacgtcggc gcggtcgtcc tgcgggaccg ccgcaagctc
     2521 gccggggacg gcgtcgtgat cgtcgtcgtc accgcggacg cgcagtcggg cgagctcctc
     2581 gcggggccgg acatcgtcac gcgcggcttc gtccacgacg agtcctcggc cgagatgctc
     2641 gaggaggccc ggcgccgcac gcgagagtcg ctggaggccg tcgcgcgcga ggaggtcggc
     2701 gaccccgcga tcctcaagca gcacgtgcgg cggacgctgg gccgctactt cttcgaggtg
     2761 acgaagcgga agccggtcat cctgccggtc atcatggagg tctagatggc gaagacgaag
     2821 aagcggaaga aggcgacgaa gcgggggagc ggcgtcgcca ccgccgggcg ccgggtggcc
     2881 gagcacctcg agccgcgcca ccaggacgcg atcggcatcg ggctcatcgt tctcggcggg
     2941 ctctcggcgc tcgggatctg gctcgacgcg gcgggtcccg tcgggcgctt cctcgacatg
     3001 ctcgcgcgag gcgccgtggg cgtcgtcggc tacgccgtcc cggtcctgct cctgtggtgg
     3061 gggctcgtcc tcgtccgcgg gacggcgcag gaggagcgcg gccggatgct cgtcggcctc
     3121 gtgttcgcgc tcgccggcgg cctcggcctc gtctcgctct tccgcggcaa cccttcgccc
     3181 gcggccggcc tctccgggtt cggcgacgcg ggcgggatcc tcggcgccgc cacggcctgg
     3241 ccgctctctc gcctcgcctc cgcgtacggc gcggccctcg tctgcctggg actcttcgtc
     3301 ctcgggatcc tcgtgttcac cgcgacgccg ctctcggcgg tgtgggagtg gttccggcgc
     3361 gagcaggcgc cggccgaggc ggccgccgcg gaggacccga tgcccgaggc ggagcccgca
     3421 ccgcggaagc ggaagcgccg tcgcctgcgg gacctcaccg acgaggaggt ggcggaggcg
     3481 acgcggtggg aggagatggg cgaagaggtc ccggcggacg cacctgccga gccggagccg
     3541 gcgccgccgc cgccgccccc cgttccgagc gggcgcggcg actacaagct tccgccgctc
     3601 aagctcctgc gaaccggccc cgggacctcg gccgcgagcc gggacgagaa ggagacgatg
     3661 gcgacgctcg agcgcacgct ccgccagttc ggcgtcgacg ccaccgtcgc ggggttcagc
     3721 cgtgggccga ccgtcaccct ctacgaggtc ggggtcgcgg ccgggacgaa ggtcaacaag
     3781 gtcctgtcgc tcggcgacga catcgcatac gccctcgcga cacccgacgt ccgcatcatc
     3841 gcgccgatcc ctgggaagtc ggcgatcggg atcgaggttc cgaaccgggt ccgcgacttc
     3901 gtgatgctcg gggacgtcct cggcgccaag gagacgaagg aggagcgcca cccgctcgcg
     3961 gtcggtctgg ggaaggacgt ccacggcaac gccgtgatgt gccgcctcca cgagatgccg
     4021 cacctgctca tcgccgggac gaccggggcg ggaaagtcct cgctgatcaa cgcgttcgtc
     4081 acctcgatcc tgatgcgggc gacccccgac gacgtcaagc tcatgctcgt cgaccccaag
     4141 cgggtcgagc tcacgcactt cgtcgacctc ccgcacctga tctctccggt cgtcgtcaac
     4201 ccgaagcgcg ccgcggaggc gctcggctgg gtcgtgcggg agatggagat gcgctacgag
     4261 atgctggcga cggtcggcgt gcgcgacatc atcggctaca acggcggtct cgccgagggg
     4321 accctgcgga tccccccggg acgcgacgcc gagttcgagc gcctcccgta catcgtggtg
     4381 gtcatcgacg agctcgccga cctcatgatg gtcgccccgc gagacgtcga agacgcgatc
     4441 tgccgcatcg ctcagatggc ccgcgccgtc gggatccacc tcgtcgtcgc gacccagcgc
     4501 ccgagcgtgg acgtcgtcac cgggctgatc aaggcgaaca tcgcctcgcg gatcgcgttc
     4561 atgacggcga gccaggcgga ttcgcgcgtg atcctcgacg tcggcggggc cgagaagctc
     4621 gtcggtcacg gggacatgct gttccagccc acgggcgcca gccgggccgc gcggatccag
     4681 ggggcgtggg tcacggagaa ggagatcgag cagatcgcgg actggtgccg ggctcagcag
     4741 gagccggtct acgtgcaggg catcgaaggg gtcgggcggc cgccgtcgga ggaggacgcc
     4801 gaggacgggg acgacgacct cctcacccag gcgatggagc tcgtcatccg ctcccagctc
     4861 ggctccacct cgatgcttca gcgcaagctc aaggtcggct tcgccagggc gggtcggatc
     4921 atggacctgc tcgaggagcg cggggtggtc ggaccctccg tcggctcgaa gccgcgtgac
     4981 gtcctgatca cctgggacga gtggctggag tcccgggagg cgcgcgcggg ctagcattgg
     5041 ccccgcgatg agccgagaga tgcccgcccg agccgtcgtc gtcgccgccg atcctggtgc
     5101 gcgcgaggcg atgcgtcgcg cgatcgacga ggtcccgaac gtcgaggtcg tcggggaggc
     5161 cgacgatccg aaggacgccg ccatcctcgc tgcgcgtgcc caccccgatc tcgtgctgat
     5221 cgcgctgtcg ccggccgacg agaacgctgc gtggggactg ccttctctcc tcggtgtcgc
     5281 tcccggcgcg cggatcgtgc tcacgctcct ggaggagtgg tcgaacgagc ggccgcagca
     5341 gggctacgac ctgcgggaga tcatcgcggt cctgcgcgag gcgcgggccg ccgacgaccg
     5401 ccccgaggga catcgccggc gggaggcgac gctctcgatg ctcgcccacg acatcctcac
     5461 gccgacgacg gccgtcgtgg gcgtcgccga cctcctgctc cgctactggg atcgcttcga
     5521 cgaccagcag cggcgtgagg gggtggccaa catcgccgag gtcggccgcg acctcgcctc
     5581 gctggtcaag ggcgtggtgc gtgccgccgc ggccgacgcc ggttcgctcg tcggcgaccg
     5641 cgagccggtc gagctggggg agctgctgga gcaggtcgtc cgcgggatgc aggcggccgc
     5701 gcaggaccac cggatcgagc tcgagaccgc ggccgagcta ccgaccgtgt ggatcgatcg
     5761 cacccggatc caggaggccg tgctgaactt cctgtccaac gcggtgaagt actcccccgc
     5821 gggcacgacc gtacgcgtcg gcgccgaggc gggggagcgg gaggttcggg tgacggtgac
     5881 cgacgagggc ccaggcatcg acccgaagga ccacgggaag ctcttcagga agttctcgcg
     5941 gctccatccc gaggagacgc cgggccacgg cctcggcctc tacctggtga aggcgatcgt
     6001 cgaggcccac ggcggccgcg tctgggtcga cggcagcccc ggcgccggct ccaccttcgg
     6061 cctcgccctg cccgccaccg gccgaaccgc tggaaccggc agatccggtc gcagggacta
     6121 gaaccgtcac cggcggggta cgacagatga cgtgcggtcc ccccgtcatg cgcgtcgtct
     6181 cccggtcctc cgccgtccgc tgctcggcct tctggcggcc ctggcggccg tctccgtcct
     6241 gagcgggttc accgcgacgg cggcgcctcg accagccccc gagcccgatc ccgtcccgtc
     6301 ttggcacgag gagacggcgc ccgtcagctt cgccggggtc gcctcctggt acggcgagtg
     6361 gttccggggg aaaccggtcg cctgtggcgg cgaaccctac gaccccgacg cgctcacggc
     6421 ggcgcatcgc tggctcccct gcgggacgaa gctccgtctc tcacacgcct cgcgcgaggt
     6481 cgtcgtcacg atcaccgacc gcgggccgtt cgtcgaaggg cgggtcctgg atctgacgcc
     6541 ggccgcgttc gcggcgctcg ccgaccccga tcgcggtctc atcggcgtcc gcgccacgct
     6601 cgtcagccca tagagcctca cgccttcgtc gctcggagcc cgtggctcct gtgcgtataa
     6661 tcgctgcgac tccccaacct ccacagggaa ttgaccatgt cggcgaacgg aacggggatc
     6721 ggtcgggcgc tccggacggc gcggctccgc cggggcaaga gcctcgagga ggcgagccgc
     6781 gagacccgcg tcaagaccga gtacctacag gcgctcgagg tcgaggcctt cgactcgctc
     6841 ctcggcgacg tctacgtccg cggcttcctc cgctcctacg cccgctacct cgggctgaat
     6901 cccgagaagg tcgtcgcgtt ctaccagagg gcgtaccgcc gggacacgcc cgcgccggcg
     6961 ccgctgcacc gggctcccgg ggtggtacgc gagccgctgc tggaaccgaa ggagcacaag
     7021 caccggaccg cgtggatcct cgccgccggg gccgcggtca tcgtgctgat ctcggccgct
     7081 gccgtcgggc tgctctcgcg cgagacgccg gcccccaccc ccgcggcccc ggcgcaggcg
     7141 gctgcggtcc cggtggctcc ggagaaggtg caggtcgaca tcgtcgcgga ggaggcggtc
     7201 ggggcctcgg tcatcgtcga cggccgcccc gagctcgggt tcaacggcct cctcgaggcg
     7261 ggcgaggcgc actcgttcga gggggagcgg tcgatcgagg tgcatctcga ctccggctat
     7321 cgcgccgaga tggtcgtgaa cggggagagc ctcggcagac cagggatgcc gaaccgtccg
     7381 tacgagcgga ccttcaccgc ggaagatttc cgggggaaga agggatagcc gcccccgcac
     7441 ctttcggcat cggctggccg aacctcgtct cggtggttcg catcgcgctc gcgcccctgc
     7501 tcgtggggct gatcctcgtc ggcgaccggc gggcctccct gatcgccgcg gcggtgttca
     7561 tcgcgggtgc ggcgaccgac cggctcgacg gctacctcgc acgccgattc gccagcgcca
     7621 cgcggaccgg agcctggctc gacccgctcg ccgacaagct gttcaccctc tcgcccgtcg
     7681 tggcgctcgc ggccgtgcgg cggttccccc tgtgggccgc cgcgatcttc ctcgtgcgcg
     7741 aggcggcggt gtccctgctg cgagcccgcc tcggggcgcg cggtcactcg atgccggcga
     7801 ccccgcttgc gaagtggaag acggccacgc agatgctcgc gatcctgctc tacatcctgc
     7861 cgctcgaccc tgcggccgac ccgctccggc tcggggtcct cgccatcgcg gtcgcgctga
     7921 cgctgtggtc gggagcggag tacttcgtcc ggagggcgcc cgcgccgtga gggccgaggt
     7981 catcgggatc ggcaccgaga tcctcctcgg cgagatcgcg aacacgaacg cacgctggat
     8041 ctccgaacgg ctggccgagg tgggcgtcga cgtgctccac caccagacgg tcggcgacaa
     8101 cctcgagcgg atcgccgatg cgttccggct cgcgctgtcc cgtgccgacg tcgtgatcgc
     8161 gacgggcggg ctcgggccga ccggcgacga catcacccgg gagggcctcg cggccgcgct
     8221 cggcgtcgag ctgcggcggg agcgggcgat cgaggatcac ctgcgcgcct ggttcgccgg
     8281 caagcggcgg acgatgcccg agtcgaacct ccgccaggcc gatctgcccg agggcgcccg
     8341 gtggatccgg ccggagctcg gaacggcgcc ggggctgatc ctcgaacgac aggggaggcg
     8401 cgtctacgtg gtccccgggg ttccggcgga gatgcgcgag atgatggagc gcgagatcct
     8461 cccggagctc cggcgcctga gcggcgccac gatccgctcg aaggtcgtca aggcatcggg
     8521 gctcgccgag gcggtggtgg gcgaacggct cgcggagctg ttcgagtcct cgacgaaccc
     8581 gacggtcgcc ttcctctcga gcgggggcga ggtgcgcgtc cggttgacgg cgaaggccga
     8641 gacggaggaa gaggcggagc ggctgatcca cccggtggcc gagaaggtgc gcggcctcct
     8701 cggcgacgcg gtcttcggcg aggacgagga gcgcctcgag gaggtcgtcg ggcgcctcct
     8761 ggcggagcgc gagctccatc tcgcctgcgc cgagtcgctg accggcggac tcctcgcggc
     8821 ccgcctcgtc tccgtcccgg acgcctcgaa gcacttctcc ggcgcggcgg tcgtctacgc
     8881 gccggacgcg aagcgcgacg tgctcggcgt ctcccaggcg acgatcgacg gaccaggcac
     8941 ggtgagcgag gaatgcgcga tcgagctcgc ccgcggcgcc cgtaaggtgt tcaaggccga
     9001 cgtggggctc tcgctcacgg gcgtggccgg gcccgagccg ctcgagggga aggagccggg
     9061 caccgtgtgg gtcggcctgt cgaccgaggc gaccgagacc gcgcggacgt tcgtcagccc
     9121 cggcgaccgt gacatggtcc gccgggccgc cacgcaggcc gcgctcgacc tcctccgccg
     9181 ccatttgctc cgtcagggat gaccgagggg aaggccctcc ggctcttcgt ctgcgtcgac
     9241 ctctcggacg aggcggccga gatcctcgga cgggtgctcg ttccctggca ggagcggctg
     9301 ccgcgggccc gatgggtcgc ccgcaggaag tggcacgtca cggtgcggtt cctcggccgg
     9361 acccagccgg acctgctgga cttcgtggag gaggcgtgcc ggggcgccgc ggcggaggtg
     9421 gagccgttcg aggtctcgct ggcgggcctg ggcgtgttcc ccggccgaac gagggcccgc
     9481 gtgctgtggg ccggcgtgga cgacccgcga ggcgcgctcg cgacgctcgc cgccggactc
     9541 gaccgccgcc tcgcgccgga gttcgagccg gagacgcgcc cgttcacccc gcacatgacg
     9601 gtggcgaggt tcaacccacc cgagcccttc cgggagcacg ccgacgccct cgaccgcacc
     9661 gagctggaga cggcgccctg ggagatcgac cggctcctgc tctaccgcag ccagctctcc
     9721 ccgaagggca gcgaatacga gctcctgcag agcttcccgg tgggctgatc ggtcagcggt
     9781 tcttctggcg gtcgagcggg acgacggtgg tgccggtggc gcggccgacg aggatcggct
     9841 cgtccagcag ctccgcggcc tcgtctccga ggtcgggctt ggcggcgatg accttccagg
     9901 cctcgaactc gcaggtgcgc gaggtgcggc cgacgcgcac gatccggccg cgcgcttcga
     9961 tgaagtcccc ggcgtagacc ggcgccttga actcgacctt ctcgtagccg acgagcaggc
    10021 cctcgtcccc gtcgtggcgg atcgccagct cggtgaccac gtcgccgaac agccgcagca
    10081 tcgtcgcccc ggccacgagg ttcccgccgt aatgcgcgtc ctcgctgccc atccgaaccc
    10141 gcagcatcac ccagtcctcg ctcatctcgc tccttccctc gggaccagca gcgcgtcgac
    10201 cgccacgacg ccatcttcgc gcacgaccag cgggttgatc tcgatcgcct cgacgggcat
    10261 cccctcgacg aggtcgatca ggcggaacag gacgtccgtg acgcggccaa gcggtagcgg
    10321 gacgccgcgg acatcttcga gcaggagccg cccccacggc gcgtcctcga gcgcgcgccg
    10381 gacgacatcc tcggatccgg ggcccgggag gacgaccgcc tgcccgagcg cctcggtcca
    10441 gatgccgccg agcccgagga gcccgcagac gccgaactga gcgtcgcgga aggcaccgac
    10501 gaggagctcg agccggccgc ggacctcctc gctcacgagg agcccggcgc cgaggcggag
    10561 gacctcggcg gcggcctccc ggaccgcctg ctccgtggcg aggccgagcc ggacgccgcc
    10621 cacctccgtc ttgtgcgcga tcccggcggc cttcgccacg acgggaaagc cgatgcgctg
    10681 cgccgcctcg acggcttcct cgacctcacg cacctcctct tcgcgcgtca cggggatccc
    10741 gatctcacgc aggaggcgct tcgccgcgtg ctcggtccag gggccgccgc cgacgtcggg
    10801 cgcgtcgagg
//
LOCUS       DATLGG010000078         9956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_208886, whole genome shotgun sequence.
ACCESSION   DATLGG010000078 DATLGG010000000
VERSION     DATLGG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208886"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VM638_02125"
     CDS             <1..181
                     /locus_tag="VM638_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11262.1"
                     /translation="SSSEPAPDVKLTVVSDPPAPPRGEQKAVDAPLCYQCGSKMQPAG
                     SCYVCGSCGATSGCS"
     gene            complement(227..1198)
                     /locus_tag="VM638_02130"
     CDS             complement(227..1198)
                     /locus_tag="VM638_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11263.1"
                     /translation="MSQATCTRCGRPLRNGVCPRGHPQRAARRRARPKRGRKLLVALL
                     ILALLAGAAWGAFRWYPERAAAQLLRGPSETFASTADAYNAAVHAFPTQPTAETLETQ
                     VSASVTTSARARPALADLQGRIEARDPVTIPVVRNRDPLPLAHRVRARMLDFAIGGLE
                     LIADMEGVSTYLQEAAPLLETADELESELGTPRTPRDVDGSAEAVRPVAGRLVSELGA
                     LTPPPELTATHESARAIAERIQADLTELDDVTGRGAEPIIRSLAQQIRRQLGSLRETL
                     LSAPEEARDAGGLGARIDHLEAVQERIVRGLLRLRNRSGVAVTVPTV"
     gene            1251..1679
                     /gene="dut"
                     /locus_tag="VM638_02135"
     CDS             1251..1679
                     /gene="dut"
                     /locus_tag="VM638_02135"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018587501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVM11264.1"
                     /translation="MDLPIRRLDPHAAAPGYAHPGDAGLDLAANVEIEVPPGGRALVG
                     TGLAVAIPEGHAGLVLPRSGLASREGLTLANAPGLIDAGYRGEIVLAVVNLDRKHAVR
                     IARGQRIAQLVLVPFAAARVVEVEELPPSERGTGGFGSTG"
     gene            1723..1798
                     /locus_tag="VM638_02140"
     tRNA            1723..1798
                     /locus_tag="VM638_02140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Gly,seq:gcc)
     gene            2074..3792
                     /locus_tag="VM638_02145"
     CDS             2074..3792
                     /locus_tag="VM638_02145"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HVM11265.1"
                     /translation="MTATPATTRTAIMLLLALTGGLLGAAPASANTLINVDTLASGSA
                     DGCTLHEAITTANTNANFGGCVRSGSGTDEITFSVNGQIELDTFLPQITQGVIIRGPG
                     ADSLEVRRAAVMEFGIFDIGLSATSQISGLTISNGRSAASGGGIRSSSSLTLVDVRVL
                     DNVVRRVENHTGSSGVGFSADGGGVWVAGALTIYESTIMRNRAEAVQNGTSNNSGMAR
                     GGCVYAGQSITLRDSTVSGNVASAEAPTTDTYNGSATAEGGGVFTRGGLIETSTIDGN
                     LARATQLGAGGFSVAHGGGIRSGFADTNVELATISGNEVRAVSGGSTPNEAGGGLFAG
                     YSFIAPQLLSLVSSTVAFNTAADGANLMTHETDSVKNTILSDPREGPSCSGTIENDLG
                     FNLMAGGTCGNVTAITASPQLLALQDNGGPTKTHNLRATSPAIDKGDGGSFDQRGEPR
                     PVDLLSIANALTGDGSDIGAFEKQDLPIDPRCEDPGVICGTQDPDVLIGTSGPDLILG
                     FGGKDTIRGGAGNDRLIGGAGNDRLYGGSGKDRLEGGSGRDYLHGGPGRDRCSGGPGK
                     DTLRSC"
     gene            complement(3853..4605)
                     /locus_tag="VM638_02150"
     CDS             complement(3853..4605)
                     /locus_tag="VM638_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11266.1"
                     /translation="MKRSSRALTSRVLALGLALALTGCARAPETSARPPMAGAGPGAS
                     PAVREITEFEDFALLERGTYSIDPDGDASTRLRVVFEVPFAWYKWIGASKSAGDGHAG
                     VGITTVRNLVSHGCRDHRPADPPVGPAVDDLATALTHLAPFEVVSPPKDVSIYGYRGK
                     HLQWRVPDMPVERSGDDLDFTPCIEGNLKSWISPTWGPYHGYTGPGYTEEFWILDVED
                     TRLMIVAKRSAGSPPQDLAELRAVLASIRIEP"
     gene            4792..7641
                     /locus_tag="VM638_02155"
     CDS             4792..7641
                     /locus_tag="VM638_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM11267.1"
                     /translation="MSRVLVLLLAASGLAAVPAAGAGTPDVVEPLSTTLSHVAVPAGS
                     DPCAVRLTLPGERHLDAACEGEGDEEAATSDADGSGALSLEARSSLQTPRWQAALAPL
                     DGSGSFVRVHGYGDVAAQDSKGRELWSRGSSSFFRDWGLDPFIVPFVLLGVSPLDPRT
                     LASERPYAVGDLNGDGKDDVAVAHYVRQRDESNQTWATSTLTVLDGRDGSTLWARRYP
                     GLLTQVVASAGGRLVVGQETGNVSTRPTGLDANGADGTTSTLHGLRFEPRGDALEAQT
                     TWTRSTGRQWVRWLAAEPIGDGLVAASWMDKPFAQATGTHGHTLLVDAATGELRWQQA
                     NTGGYPRVLRHDASRDAVLIAELGQPYPPYRYDLVTIPLDGGASSRVTRADSILLSLT
                     VADLSRDGAAEWVAGEAIVTTRPGTAPSITGGRVVALEGGGGVRWSRETRCPEDVPQL
                     CGLATLPLTYGLHVDPGRSVVVDASFSGGRSRLRGLSAATGTVLWDRTSDPAYPLYLA
                     PAAGHGRAAVLAATPGLIARAYDVATGATLMSAPVPFELHAVEAADITGDGRKEVLAG
                     GEGGVVFAFDVGGPVPRVLWAAEAGRMVHKIAVTDLGGDGKREVVVAAASALAVFDLK
                     GEPVASRTVEYPHPGDYVWTFALGDVSGDDRTDVVVPGRGLAAYDGRTGARLWQYAGT
                     NPDIPAYFSEAVITPDRRVVSQWLTRSGVPFLNARSHQVVAALDGRTGQIAWTRVVPS
                     KFGIVNLWRSVAIDPDHSCGPGATVAVTWDDSPGVVGWVSRTDFYDSQTGTLCFTSGP
                     HAEQIVHMGTEHFPGTGMLQWNWYHVAHLRPGGVTATYYHPVNAATSEVAMADFGSLG
                     KRFLRAWTRVHAYGAETPTGAAGNDPPEHARWEAFDTGRLLPADLDADGSDEVVAVGF
                     DWRGYATVASFSSVGVFSSSFLPRGIAVLEAGPAS"
     gene            7658..9649
                     /locus_tag="VM638_02160"
     CDS             7658..9649
                     /locus_tag="VM638_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11268.1"
                     /translation="MPARTLLIALGLTAFLGTGGAPGPSLPLLSPYGGEQVPVSIREA
                     MAATQETDVALPARVQDWEPSSSEIRSFATTDHSDPQAGPRQGTTRWRTVGSTGNAAE
                     TWFTITKGGRILDLGGRYVNYSDDQGLTWKSVRPQEELVNAEGSVIQAPNGDIVAITW
                     DPYSGDRVLSYKYNAAEDAWKYMYQAVHTPFWDRPSIEVVPGDFTDRLGNKVPYITFT
                     NGFPHDPWQFSYDGLNYTEISSPSRDRGPAISSWLDVKPDPMFDYIQPNNALDQGLIP
                     KFAPLGDGRAWYQNLLFTADDMKWHPWTLPGGGTIQGALQVDSRGWLHNVLRVGSGFR
                     YRISPDGGRTWNSIDVAGGSAGDFRANGAAGVAAVWAINGRQDLAYKIDITTPQPKLL
                     RRYVVGLGDDVRTGGIGFYGLTGGHRFDFSSIGVFPDGRIAVVFMDSKTKMPFPTLGN
                     AATCAVKDVPPQPVGQSPCEVVAPQLAIEVDTTIPKLREGFEGGIGAWQQGADVPLDP
                     NTGQPVAWSITPSSEEASEGTASARFDLDGRQDDGTIWLTRPVSLPPGKSHELTLSVK
                     AWSPAASPANEISDLVLYLGSKPPDGEASFDPASGPDRAALREPLYQQAGWRRYELKA
                     TIDPGLSGVVYLAAGVSATWETEITHYLDDVSLEVTSRP"
BASE COUNT         1506 a         3469 c         3543 g         1438 t
ORIGIN      
        1 gtcctcgtcc gagccggcgc cggacgtgaa gctcacggtg gtgagcgacc cgcccgcgcc
       61 gccccgcggc gagcagaagg cggtcgacgc gccgctctgc taccagtgcg gctcgaagat
      121 gcagccggcc ggatcctgct acgtctgcgg cagctgcggc gccaccagcg gctgctcctg
      181 agcggcggtc acgaggcccg gcggaggtcc ccctcgccgg gcctcgtcac accgtgggaa
      241 cggtgacggc gacgcccgat cggttccgca atcgaaggag accgcggacg atccgctcct
      301 gaaccgcttc gaggtggtcg atcctggcgc cgagcccgcc cgcgtcgcgc gcctcctcgg
      361 gtgccgagag cagggtttcg cgaagggatc cgagctggcg acggatctgc tgcgcgagcg
      421 atcggatgat cggctccgcg ccgcggcccg tgacgtcgtc gagctccgtc agatcggcct
      481 ggatccgttc ggcgatcgcc cgggcggact cgtgcgtcgc ggtgagctcc ggaggggggg
      541 tcagggcccc gagctccgag acgagacggc cggccaccgg ccggaccgcc tcggccgacc
      601 cgtccacgtc gcggggcgtc ctcggcgtcc cgagctcgct ctcgagctcg tcggcggtct
      661 ccaggagcgg cgccgcttcc tggaggtagg tcgacacccc ctccatgtcc gcgatcagct
      721 cgaggccacc gatcgcgaaa tcgagcatgc gcgcgcggac ccgatgggcg agcggcagcg
      781 ggtcgcggtt gcgcacgacc gggatcgtca ccgggtcccg ggcttcgatc cgcccctgga
      841 ggtcggcgag ggccggacga gctcgggcgg acgtcgtcac cgacgccgag acctgcgtct
      901 ccagggtctc agccgtgggt tgcgtgggga aggcgtggac ggccgcgttg taggcgtcgg
      961 cggtggaggc gaacgtctcc gaggggcctc gcaggagctg cgccgcggcg cgttcggggt
     1021 accagcggaa cgccccccag gccgccccgg ccagcagcgc gaggatcagc agggcgacca
     1081 ggagcttccg ccctcgcttc ggacgcgcgc gccggcgtgc cgcccgctgc gggtggcctc
     1141 tcgggcagac cccgttgcgc agcggccgcc cgcatcgcgt gcacgtcgcc tgggacactc
     1201 cggcggagga tagccgacgg ccgtccgcac ccaccggata ccctgacggc gtggaccttc
     1261 cgatccggcg gctcgacccc cacgccgcgg cgcccggcta cgcccatccg ggcgatgccg
     1321 gcctcgacct cgccgcgaac gtcgagatcg aggtcccgcc ggggggacgg gccctcgtgg
     1381 ggacgggcct ggcggtcgcg atccccgagg gccatgcggg gctggtcctg ccgaggtccg
     1441 gcctcgcctc ccgggagggg ctgacgctcg cgaacgcccc cggcttgatc gacgccggct
     1501 accggggcga gatcgtgctg gccgtcgtga acctcgaccg gaagcacgcg gtccggatcg
     1561 cgcgcggtca gcgcatcgct cagctcgtgc tcgtgccgtt cgcggcggcg cgggtcgtcg
     1621 aggtcgagga gctcccgccg agcgagcggg gcaccggcgg cttcggctcg accggctagc
     1681 cctggagccg gtcccaggcg gactggtaaa ctagcggctc acgcggacgt ggctcagttg
     1741 gtagagcacc accttgccaa ggtgggggtc gcgggttcga atcccgtcgt ccgctccacg
     1801 aaccagcagg tcagcggttt tttcccgagc gggtacgccc ggggtccctt ccctctccgc
     1861 ggagccacgt gtcggatcgt ggcattgcca gcggcaggcc aaccggtgaa cgtgcgatgc
     1921 acgcgaccgt ggtggacgcg gacgcctgtg agcatgcaac ggcagggatc cgggtccgcc
     1981 gtgtcgaata agagccggga cgcgtccgtg tggcgcggat ctgcccatcc gacacgtccg
     2041 tccgggtgtg tcccaccgag cgaggtcgag aacatgaccg ccacaccagc caccacacgc
     2101 accgccatca tgctcttgct cgctctcacg gggggcttgc tcggcgccgc acccgcgtcc
     2161 gccaacacgt tgatcaacgt cgacacgctg gcgtcgggga gcgccgacgg ctgcaccctc
     2221 cacgaagcga tcaccacggc gaatacgaat gcgaacttcg ggggatgcgt gcggagtgga
     2281 tcggggaccg atgagatcac cttctcggtg aacggccaga tcgaactcga caccttcctc
     2341 ccccagatca cgcagggtgt gatcatccgc ggaccgggcg ccgactcgtt ggaggttcgc
     2401 cgcgcggccg tcatggaatt cggcatcttc gacatcggtc tcagcgccac gtcgcagatc
     2461 agtggcctca cgatctccaa cggccggtcc gcggcctccg gcgggggcat ccgcagctcc
     2521 agctccctga cgctggtcga cgtgcgcgtc ctcgacaacg tcgtccggcg cgtcgagaac
     2581 cacaccggta gcagcggcgt ggggttctct gccgatggcg gtggggtttg ggtcgccgga
     2641 gcgctcacga tctacgagag cacgatcatg cgcaaccggg ccgaggctgt gcagaacggg
     2701 acgagcaaca acagcgggat ggcgcgaggg ggctgcgtct acgcgggcca gtcgatcacc
     2761 ctgcgtgaca gcaccgtcag cggcaacgtg gcctccgccg aggcgcccac gaccgacacg
     2821 tacaacggga gcgcgaccgc ggagggcggt ggcgtattca ctcggggcgg cctcatcgag
     2881 acgtcaacca tcgacgggaa cctggccagg gcgacccagc tcggcgccgg ggggttctcg
     2941 gtggctcacg gcggcgggat ccgcagcggt ttcgcggaca ccaacgtgga gctcgctacg
     3001 atcagtggga acgaggtacg ggccgtgagc gggggttcca cccccaacga ggccggaggc
     3061 ggcctgttcg cgggatactc cttcattgcg ccgcagttgc tctcgctggt gagcagcacg
     3121 gtcgccttca atacggctgc cgatggagcc aacctcatga cccacgaaac cgacagcgtg
     3181 aagaacacga tcctctcgga tccgcgcgag gggcccagtt gctccggcac gatcgagaac
     3241 gacctgggct tcaatctcat ggccggaggg acctgcggga acgttaccgc tataaccgcc
     3301 tcaccccagc tcctggcgct ccaggacaac gggggtccca cgaagaccca caacctgcgg
     3361 gcgaccagcc cggcgatcga caagggtgat ggagggagct tcgatcaacg cggtgaaccg
     3421 cgcccggtcg atctgttgtc gatcgcgaac gccctcaccg gggatggctc agacatcggg
     3481 gcgttcgaga agcaggacct gccgatcgac cctcgctgcg aagacccggg tgtcatctgc
     3541 gggacgcagg atccggacgt gctgatcggt acatccggtc cggacctcat cctcgggttc
     3601 ggcgggaagg acacgatccg aggaggcgcc ggcaacgaca ggctgatcgg cggcgcaggg
     3661 aacgaccgac tgtacggggg ttcaggaaag gatcgtctcg agggcggctc cggcagggat
     3721 tacctccacg gtgggcccgg acgcgatcgc tgcagcggcg gaccagggaa ggacaccctc
     3781 aggagttgct gatcctgtga ggggcaaccg cgcgggggtg gatcgcgggt gcgtgactcg
     3841 gacggcgcaa cgtcagggtt cgatccggat ggaggcgaga acggctcgca gctcggccag
     3901 gtcctgcggt ggtgacccgg cagatcgctt ggccacgatc atcagacggg tgtcttcgac
     3961 gtcgaggatc cagaactcct cggtgtaccc gggaccggta tagccgtgat acggccccca
     4021 ggtgggggag atccagctct tgaggttgcc ctcgatgcac ggggtgaagt ccagatcgtc
     4081 cccggagcgc tcgaccggca tgtcggggac cctccactgc aggtgcttcc cgcggtagcc
     4141 gtagatgctc acgtctttcg ggggtgatac gacctcgaac ggagccagat gggtgagcgc
     4201 cgtcgcgagg tcgtcgaccg ccggcccgac gggtggatcg gcggggcggt ggtcgcggca
     4261 gccgtgactc acgaggttcc tgacggtggt gatccccact ccggcgtgtc catctccagc
     4321 ggacttcgat gcgccgatcc acttgtacca ggcgaaaggg acctcgaaga ccacgcggag
     4381 gcgcgtcgag gcatcgccgt ccgggtcgat cgagtaggtc ccgcgttcga ggagggcgaa
     4441 atcttcgaac tccgtgatct cccggacggc cgggctcgca ccgggcccag cccccgccat
     4501 cggcgggcgg gcggacgtct cgggggcccg tgcgcatccc gtcagcgcga gcgccaaccc
     4561 caaggccagg acgcggctcg tcagcgctcg cgaagatcgc ttcatcgaag tatcaacgct
     4621 ggcacgggcg gatctgccta cacctgatcc ctcgatccga acgcagtgaa gccccctggc
     4681 gaggaaggcg cgtcgggccg gatcagatct gcggcaggag ttccgagcca tggcgtcgaa
     4741 cccttcccgc cgatgcagat ccccggttcg tcaacgcgaa ggaggccgtc ggtgtctcga
     4801 gtcctcgtcc tgctcctggc cgcaagcggc cttgctgccg tcccggccgc cggagccggc
     4861 acgcccgacg tcgtggagcc gctgtccacg acgctgtccc acgtcgcggt cccggctggg
     4921 tccgacccgt gcgccgtgcg cctcacgctg cccggtgagc ggcacctgga cgccgcctgt
     4981 gagggggagg gcgacgagga agctgcgacg agcgacgccg acggctcggg ggcgctctcc
     5041 ctcgaagctc gcagcagcct ccagaccccg cgctggcagg cggcgctcgc gcccctcgac
     5101 ggatccggct cgttcgtgcg tgtgcatggg tacggggacg tcgccgcgca ggactcgaag
     5161 ggccgggagc tgtggagccg cggctcgtcg tcgttcttcc gcgactgggg cctggatccc
     5221 ttcatcgtcc cgttcgtcct cctcggcgtg tcgcccctcg acccgcggac cctcgcctcg
     5281 gagcgtccct acgccgtcgg cgacctgaac ggtgacggga aggacgacgt cgccgtcgcc
     5341 cactacgtcc ggcagcggga tgagagcaac cagacgtggg ccaccagcac cctcaccgtg
     5401 ctcgacggcc gggacggctc gacgctgtgg gcgaggcgct acccggggct gctcacccag
     5461 gtggtcgcct ccgccggcgg ccgcttggtg gtcggccagg agaccggcaa cgtgtccacg
     5521 cgccccaccg ggctggacgc gaacggtgcg gatggcacca cgtcgaccct ccacggcctg
     5581 cgcttcgagc cccgcggtga cgcgctggaa gcgcagacga catggacgcg ctcgaccggc
     5641 cgccagtggg tccgctggct cgccgccgag ccgatcggcg acggcctggt ggcggcttcc
     5701 tggatggaca agccgttcgc ccaggccacg gggacgcacg gccacacgct cctggtcgac
     5761 gccgccaccg gcgagctgcg ctggcagcag gccaacaccg ggggctaccc gcgggtcctg
     5821 cgacacgatg ccagccgcga tgcggtgctc atcgccgagc tcggccagcc gtacccgccg
     5881 taccgctacg acctcgtcac catccccctc gacggcggag cctcgtcgcg agtcacgagg
     5941 gcggactcga tcctgctctc gctcacggtg gcggacctga gccgcgacgg cgcggcggag
     6001 tgggtcgccg gtgaggcgat cgtcaccacc cgtccgggga cggcaccgag catcacgggg
     6061 ggtcgggtcg tcgcgctcga ggggggcggc ggcgtcaggt ggagtcgcga gacgcgctgc
     6121 ccggaggatg tcccgcagct ctgcggcctc gccaccctgc cgctgaccta cgggctgcac
     6181 gtcgatccgg gacggtcggt cgtggtggac gcatccttca gcgggggccg aagcaggctc
     6241 cgcgggctga gcgccgctac cggcacggtc ctctgggacc ggaccagcga ccccgcctac
     6301 ccgttgtacc tcgcccccgc cgcgggtcac ggtcgggctg cggtgctcgc cgccaccccc
     6361 ggactgatcg cgagggccta cgacgtggcg accggagcga cgctgatgtc ggcccccgtc
     6421 ccgttcgagt tgcacgcggt cgaggctgcc gacatcaccg gtgatggccg caaggaggtc
     6481 ctcgccggcg gcgagggcgg ggtcgtcttc gccttcgacg tcggggggcc ggttccccgc
     6541 gtgctgtggg ctgccgaggc gggcaggatg gtccacaaga tcgccgtgac cgacctcggc
     6601 ggcgacggga agcgggaggt cgtggttgcg gctgcctcgg cgctggcggt gttcgatctc
     6661 aagggtgagc ctgtcgcctc gagaacggtc gaatacccgc accccggcga ctacgtctgg
     6721 accttcgcgc tcggtgatgt cagcggcgac gaccgcaccg acgtcgtcgt tcccgggcgc
     6781 ggcctggccg cttacgacgg gcggacgggg gcgcgtctgt ggcagtacgc cggcacgaac
     6841 ccggatatcc ccgcctactt ctccgaggcg gtgatcacgc ccgatcgccg ggtcgtctcc
     6901 cagtggctga cccggtccgg ggtcccgttc ctgaacgcgc ggagccatca ggtcgtcgcg
     6961 gccctcgacg ggcggacggg tcagatcgcc tggacccgtg tcgtcccgag caagttcggg
     7021 atcgtcaacc tgtggcggag cgtcgcgatc gatccggacc attcctgcgg cccgggggca
     7081 accgtcgcgg tgacgtggga tgacagtccc ggcgtggtcg gatgggtatc ccgaaccgac
     7141 ttctacgact cgcagaccgg gacgctgtgc ttcacctccg gcccccacgc ggagcagatc
     7201 gtccacatgg ggaccgagca cttccccggg accgggatgc tccagtggaa ctggtaccac
     7261 gtggcgcacc tgcgccccgg cggggtaacg gctacctact accacccggt gaacgcggcg
     7321 acctccgagg tggcgatggc cgacttcggc agcctcggga agaggttcct gcgcgcctgg
     7381 acccgagtgc acgcttacgg agccgagacg ccgacgggag ccgcgggcaa cgacccaccc
     7441 gagcatgctc gttgggaggc gttcgacacg gggcggttgc tgccggccga cctggacgcc
     7501 gacggatccg acgaggtcgt ggccgtgggc ttcgattggc gcggctacgc gaccgtcgcg
     7561 agcttctcct ccgtgggtgt gttcagctcg agcttcctcc cgcgagggat cgccgtcctc
     7621 gaggcgggtc ccgcctcttg aaccgagagg agccaagatg cctgcccgaa ccctgctgat
     7681 cgccctcggc ttgaccgcgt tcctcgggac cggcggggct ccgggaccca gcctcccgct
     7741 cttgtcgccc tacggcggtg agcaggttcc ggtcagcatc cgggaagcga tggcggcgac
     7801 gcaggagact gacgtcgccc tgccagctcg cgtgcaggac tgggagccca gctcatccga
     7861 gatccgctcg ttcgcgacga ccgatcacag cgatccgcag gcggggccgc gtcaaggcac
     7921 gacgaggtgg cggacggtcg gcagcacggg caacgcggcc gagacgtggt tcacgatcac
     7981 gaagggtggc cggatcctcg acctcggcgg gcgctacgtg aactactccg acgaccaggg
     8041 cctgacctgg aagtccgtcc gccctcagga ggagctcgtc aacgccgagg gatccgtcat
     8101 ccaggccccg aacggggaca tcgtggcgat cacctgggat ccctactccg gggaccgagt
     8161 cctctcgtac aagtacaacg ctgcggagga cgcctggaag tacatgtacc aggcggtcca
     8221 cacgccgttc tgggaccgtc ccagcatcga ggtggtcccg ggcgacttca ccgaccgcct
     8281 tggcaacaag gtcccgtaca tcaccttcac caacggcttc ccccacgacc cgtggcagtt
     8341 cagctacgac gggctgaact acacggagat ctcgagcccc tcccgcgacc gaggcccggc
     8401 catcagctcg tggctcgacg tcaagccgga cccgatgttc gactacatcc agcccaacaa
     8461 cgcgctcgat cagggcctga tcccgaagtt cgcgcccctg ggcgacggcc gtgcctggta
     8521 ccagaacctc ctgttcacgg cggacgacat gaagtggcac ccctggacac tgcccggagg
     8581 aggaacgatc cagggagcgc ttcaggtcga ctcccggggg tggctccata acgtgctccg
     8641 tgtcgggagc ggcttccggt accggatctc gccggacggg ggacggacgt ggaactcgat
     8701 cgacgtcgca ggcggcagcg ccggcgattt ccgggcgaac ggcgccgccg gcgtcgccgc
     8761 ggtctgggcg atcaacggcc ggcaggacct cgcgtacaag atcgacatca ccacgcccca
     8821 gccgaagctc ctccgccgct acgtggtcgg cctcggcgac gacgtccgca ccggcgggat
     8881 cgggttctac ggactgaccg gggggcaccg cttcgacttc tcgagcatcg gcgtcttccc
     8941 ggacgggcgg atcgcggtgg tcttcatgga ctcgaagacg aagatgccgt tcccgacgct
     9001 cggcaacgcg gccacgtgcg cggtcaagga cgtcccgccg cagccagtgg gtcagtcgcc
     9061 ctgcgaggtc gtcgccccgc agcttgcgat cgaggtcgac accacgatcc cgaagctccg
     9121 cgaaggcttc gaggggggca tcggtgcctg gcagcagggt gccgacgtgc cgctcgatcc
     9181 gaacaccggc cagccggtgg cgtggagcat caccccctcg agcgaggaag cgtccgaagg
     9241 gacggccagc gcccgcttcg acctcgacgg gagacaggac gacggcacga tctggctgac
     9301 gcgcccggtc tccctaccgc cggggaagtc tcacgagctg acgctgagcg tgaaggcctg
     9361 gagtccagct gcgagcccgg cgaacgagat ctccgacctc gtgctctacc tcgggagcaa
     9421 gcccccggac ggcgaggcat cgttcgatcc cgccagcggc cccgaccgag cggccctgcg
     9481 agagcccctg taccagcagg ccggctggcg ccggtacgag ctgaaggcga cgatcgatcc
     9541 cgggctctca ggcgtggtct acctcgcggc cggggtcagc gcgacctggg agaccgagat
     9601 aacgcactac ctggacgacg tctcgctcga ggtgacgtcg cgcccctgac gcgaccccgg
     9661 tcgcctggag cgaggacgcg cccgaggaag acccgagcgc gatcgacggc agtgctgcgc
     9721 ggctataatg cgcgaactcc gatggggagt agccccacgc ggcggcgtcg tcatcccggc
     9781 ctcggcccgg cccgccaccc gcacgggcgg gcgagacctt cggtcgtctt cggcatggga
     9841 ggtagctgtg tgggtcttgc tgggtagggg atcggtccaa ccatgatggc tcttgcgccg
     9901 ctggctgcgg taccgaccca gggcaggttc gcccagatcg aggtcccggc gtgggg
//
LOCUS       DATLGG010000079         8547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_192575, whole genome shotgun sequence.
ACCESSION   DATLGG010000079 DATLGG010000000
VERSION     DATLGG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192575"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VM638_02165"
     CDS             <1..1000
                     /locus_tag="VM638_02165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HVM11269.1"
                     /translation="VARNLAEWCAVDLRGEGRELERVAESPDGGARPKTGTEVVVPLV
                     GREGPLGALTFGWDAPDRTASPEELGLAEDLCRRAALAVENARLYAERTHVARILQQS
                     LLPDRLPAIEGLEVDARLHAAGQAVGGDFYDLFEIGDGRWGFVLGDVCGKGVDAARLT
                     ALTRYTVRAASMRIASPDGVLEVLNDVLLRDRAGDVNLCTVAFAVITVEEGRVTLELT
                     NGGHPLPLRIAGGRVDSAGGPGTILGAFREPDLTTLRLTLGRGDTLVFYTDGVIEAKD
                     PAREVFGEPRLRDLLAELRDAPPDEVTAAIVEAALRFQAGKVRDDIAVVAFRVPSA"
     gene            complement(1006..1194)
                     /locus_tag="VM638_02170"
     CDS             complement(1006..1194)
                     /locus_tag="VM638_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11270.1"
                     /translation="MDRSPAEQELLDVYDRLAGVLRDHAADLQPYQRANATKALAALW
                     QVANGLDHDPGQVYDLGV"
     gene            complement(1194..2567)
                     /locus_tag="VM638_02175"
     CDS             complement(1194..2567)
                     /locus_tag="VM638_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVM11271.1"
                     /translation="MREVEVAESPYWNPRHETMPREELEALQVRKLRNVVAWADANVP
                     WHAKRLRDAGVTADDISSLDDLRRIPFLTRDDWMQGQLEAPPYGPFLAAPEETAVRYH
                     MTSGTTGRTPIRVLDSKKDWEWIAEMWCYGLWGFGIRPSDVVFVAFGYSTFIGFWGLH
                     YACEKIGALTLPGGAMTTDARVKQIVEMGATVVASTPTYALRMAQEAKQLGIDLANGP
                     VQRLILSGEPAGSIPATKKLIEELWGAKAGDTAGMTELGTIMMFECEEQPGGTHVIED
                     HYIEEVIDPETDEPVPYGEMGERVVTSFGRGFIPVLRYRTRDFVVKVPHDRCTCGRTF
                     DLYEGGIRGRVDDMKLVRGTNVYPRAVEAIIREHPEIDEFQIHLYTAEGIRDEIEVLA
                     EIPDESVDANEVLGALQRELAESHEGLRFGVKKVDVGTLPRFELKAKRVLDERIVIGT
                     EGERRQT"
     gene            2704..3822
                     /locus_tag="VM638_02180"
     CDS             2704..3822
                     /locus_tag="VM638_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM11272.1"
                     /translation="MSFSLSAEQREVLEEALRLGRELAPRPAAEPGRLNRPLIRALGE
                     QGLLSRVFPEDGEVAAIDLCLLREGLAQGSTEAETALALQGLGAYPILQSGSGATRDR
                     WIPAVRKGEAVAAFALTEPEHGSDAAALELAAAPDGDGWRLSGEKRWISNAPDADVYT
                     VFARTTPDAGAKGVTAFAVSGDAEGLSGESLDLLAPHAIGALRFDGVRVGPEDVLGEV
                     DGGFRVAMRTLDLFRPSVGAFAVGMAQAALDLAVAHAGERRSMGKLLREHQAVSQKLG
                     EMAARLEAARMLVYAAASAYDRGERVTKIAAMAKLVATETAQWVVDEAVQIHGAVALE
                     RGHPLEHLYRDVRAPRIYEGTSEIQREIIARELYRREE"
     gene            3823..4338
                     /locus_tag="VM638_02185"
     CDS             3823..4338
                     /locus_tag="VM638_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11273.1"
                     /translation="MDLYMIVLRIVHILAGVFWVGSAAMFFMYVQPTAKALGPAGAPF
                     MTHLVQQKRVPVVLLWCAGTTITAGLLLYWRTSGGFDTDWITSETGMAFTIGGLAAIV
                     AFMLGLFIAKPAADRMGAVAAQAAAAGGPPSQEQAAQIAASQATLRKIGITNLTLLTL
                     AVILMAVARYL"
     gene            complement(4343..4756)
                     /locus_tag="VM638_02190"
     CDS             complement(4343..4756)
                     /locus_tag="VM638_02190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVM11274.1"
                     /translation="MKASSPHRMLNPSTLPRPSGFSHAVVPTPGRTIYLGGQIGAAQD
                     GRLAGSTVVEQLDQACSNVIEALLAAGARAEHLVSIQIFVTSLMEYRTSLPAIGEVWQ
                     RHFGRHYPAVSLFEVSALFEPGAKVELVCVAVVPA"
     gene            complement(4753..5580)
                     /locus_tag="VM638_02195"
     CDS             complement(4753..5580)
                     /locus_tag="VM638_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HVM11275.1"
                     /translation="MSPHRASAPFTDDWKHLRFEVADGIGTITFDRPERLNALTFDSY
                     ADFRDLLAELPHRDDVRVVVLTGEGRGFCAGGDRDDIIGALQGAGAKELLEFTRMTGA
                     VVQHMRELPQPIVAAVNGVAAGAGSVIALAADVRILARSASFAFLFTRVGLAGADMGS
                     AYLLPRVIGLGRATELLLLGEDVPAERAVEIGLAREVVEDAELVERATSLARRLAEGP
                     ALAHAATKLLLAREQDMPLAGALELEAMTQALLMSSEDHREFYRAFTEGRRPTWRGR"
     gene            complement(5580..6767)
                     /locus_tag="VM638_02200"
     CDS             complement(5580..6767)
                     /locus_tag="VM638_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM11276.1"
                     /translation="MTQVSSRPQVDPRDLLALDHLLSDEEKAVRNTVREWVGSRVLPE
                     IAEWFERGEIPSQQLAKELGSMGLLGMHLEGYGTAGASAVMYGLACLELEAGDSGIRS
                     LVSVQGSLAMFPIWAYGSEEQKQEWLPRMAAGEAIGCFGLTEPDYGSNPAGMRTFARR
                     DGDDWILSGTKMWITNGGVADIAVVWAQTDDGIRGFLVPRGVDGFTTKDIERKLSLRA
                     SVTSELIFDDVRLPGDAVLPGVRGMKGPLSCLNEARYGIVWGSMGAARACVETAVDYA
                     KTRIQFDKPIGAFQLTQRRLVDMAVELHKGLLLALHLGRMKDEGRTAPEHVSFGKLNN
                     VREALAICREARGILGANGVTLEYPVIRHMNNLESVVTYEGTNEVHTLILGKALTGLD
                     AFE"
     gene            6880..8532
                     /locus_tag="VM638_02205"
     CDS             6880..8532
                     /locus_tag="VM638_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HVM11277.1"
                     /translation="MKRTIPILLAAFVTLAVLPGPAAGSATITFSGSRQVRGDLGDMG
                     ADPLDGARTALDAFADELGVEPSRFSLDEVRRSIVGIHVRGVERRGGVPVENTAVAVH
                     AVDGRIVQIDAHGSDLRGSAAGVAISRHAAVTAALAHAGVARTLLPPRTQRLLVASEG
                     RLVDVWRVPVLSLEPAVALTVDVDAASGRIVRAVDDRLHAEGQATVFDPSPTVTLRDT
                     TLRQPGVDRLGVDTDLPDERLNRALRLLPVRDVASATQDLLAGRLHGPYVDVYGAPGL
                     DPFGGWFYQRHDPRFETLMAYTHIDRIQRYFQSLGFVGSAAINAEPQWVITHRVEGYD
                     NSFFQPANDLILFGAGGVDDAEDAEVVLHELGHAIHYDQVPNWGGHHQAGAMGEGFGD
                     FLAAAYYAGSISGGFGDTCVADWDATSYSPANPPCLRRVDTAKRFPQNMTNNSVHADG
                     EIWSGFFWDLRQLLKCPAEPPEDDPEWETDPECKPPRNDSQIQSDRVLKLLLTSHEFL
                     TNGATFGRAVAALRTAADAIGRPEWKALVDQAAKGRGLPLNP"
BASE COUNT         1209 a         3053 c         3018 g         1267 t
ORIGIN      
        1 cgtcgcacgg aacctcgccg agtggtgcgc cgtcgacctg cgtggcgagg gtcgcgagct
       61 ggagcgggtc gccgagagcc ccgacggggg ggcgcggccg aagacgggaa ccgaggtcgt
      121 cgtgcccttg gtcggacgcg agggtccgct cggcgccctc acgttcgggt gggacgctcc
      181 ggaccggacg gcctcgcccg aggagctggg cctcgccgag gacctctgcc ggcgggccgc
      241 cctggcggtg gagaacgccc ggctgtacgc ggaacgcacg cacgtcgcgc gcatcctcca
      301 gcagagcctc ctgcccgacc gcctcccggc gatcgaaggg ctcgaggtcg acgcccggct
      361 ccacgcggcc ggccaggccg tcggaggcga cttctacgac ctcttcgaga tcggcgacgg
      421 tcgctggggg ttcgtgctgg gggacgtgtg cgggaagggc gtggacgcgg cccgcctgac
      481 cgcgctcacc cgctacaccg tccgggccgc gagcatgcgg atcgcctccc ccgacggcgt
      541 cctcgaggtc ctgaacgacg tcctgctccg cgaccgcgcc ggcgacgtga acctctgcac
      601 ggtggcgttc gccgtgatca ccgtcgagga ggggcgggtg acgctggagc tcacgaacgg
      661 cgggcacccg ctcccgctgc ggatcgccgg cggtcgcgtc gactcggcgg gcggcccggg
      721 cacgatactc ggcgccttcc gggagccgga cctgaccacc ctcaggctca ccctcggccg
      781 gggcgacacg ctcgtcttct acaccgacgg cgtgatcgag gccaaggatc ccgcgcggga
      841 agtcttcggc gagccccggc tccgcgacct tctggccgag cttcgggacg ccccgcccga
      901 cgaggtgacc gcggcgatcg tcgaggccgc gctgcgcttc caggcgggca aggtccgcga
      961 cgacatcgcc gtcgtcgcct tccgcgtccc ctcggcctga gcgcgctaca ccccgaggtc
     1021 gtagacctgg ccggggtcgt gatcgaggcc gttcgcgacc tgccagaggg cggcgagggc
     1081 cttcgtcgcg ttcgcgcgct ggtacggctg gaggtcggcg gcgtggtcgc gaaggacacc
     1141 ggccagccgg tcgtagacgt ccagcagctc ctgctcggcg gggctccgat ccatcacgtc
     1201 tgcctcctct ccccctccgt cccgatcacg atccgctcgt cgaggacccg cttcgccttg
     1261 agctcgaacc gcggcagcgt cccgacatcg accttcttca cgccgaaccg caggccctcg
     1321 tgcgactcgg ccagctcgcg ctgcagcgcc ccgaggacct cgttcgcatc gacgctctcg
     1381 tcggggatct cggccagcac ctcgatctcg tcgcggatcc cctcggcggt gtacaggtgg
     1441 atctggaact cgtcgatctc ggggtgctcg cggatgatcg cctcgaccgc gcgcgggtag
     1501 acgttggtcc cgcgcacgag cttcatgtcg tccacgcggc cgcggatccc gccctcgtag
     1561 aggtcgaagg tccgcccgca ggtgcaccgg tcgtgcggca ccttcacgac gaagtcgcgc
     1621 gtgcggtagc gcagcaccgg gatgaacccg cgcccgaacg aggtcaccac ccgctccccc
     1681 atctcgccgt agggcaccgg ctcgtcggtc tcggggtcga tgacctcctc gatgtagtgg
     1741 tcctcgatga cgtgcgtgcc gccgggctgc tcctcgcact cgaacatcat gatcgtgccg
     1801 agctcggtca tcccggccgt gtcgccggcc ttcgcgcccc agagctcctc gatcagcttc
     1861 ttcgtcgccg ggatcgagcc ggcgggctcg ccggagagga tcagccgctg cacgggtccg
     1921 ttcgcgaggt cgatcccgag ctgcttcgcc tcctgcgcca tccgcagcgc gtacgtcggc
     1981 gtcgatgcca cgacggtcgc gcccatctcc acgatctgct tcacgcgcgc gtccgtcgtc
     2041 atcgcgccgc cggggagggt gagcgcgccg atcttctcgc acgcgtagtg gaggccccag
     2101 aagccgatga aggtcgagta gccgaacgcg acgaagacga cgtccgacgg acggatgccg
     2161 aagccccaca ggccgtagca ccacatctcg gcgatccact cccagtcctt cttcgagtcg
     2221 aggacgcgga tcggcgtgcg cccggtcgtc ccggaggtca tgtggtagcg aaccgcggtc
     2281 tcctcgggtg ccgcgaggaa cggaccgtac gggggagcct cgagctggcc ctgcatccag
     2341 tcgtcgcgcg tgaggaacgg gatccgccgg aggtcgtcga gcgaggagat gtcgtcggcg
     2401 gtcacaccgg cgtcgcggag gcgcttcgcg tgccagggga cgttcgcgtc ggcccacgcc
     2461 acgacgttgc ggagcttgcg cacctggagg gcctcgagct cctcgcgcgg catcgtctcg
     2521 tgccgcgggt tccagtacgg cgactcggcc acctcgacct ccctcacccg ggtacgggcg
     2581 aatcctaccg ctcgcgctcg cccgacggca ccttcggacc gtcggaggct cgcttcatcc
     2641 ggaccggatc atcggcggag ccgatctgct gactcccccg gcttggaccg ctagagttcg
     2701 gccgtgagct tctcgctgtc cgcggagcaa cgggaggtgc tcgaggaggc cctgcggctg
     2761 gggcgagaac tcgcccccag gccggccgca gagccgggcc gcctgaaccg cccgctcatc
     2821 cgagccctcg gggagcaggg gctcctgtcg cgggtcttcc ccgaagacgg cgaggtcgcg
     2881 gccatcgatc tctgtctcct gcgcgagggg ctggcacagg gctccaccga agccgagacg
     2941 gcgctcgcgc tccaggggct cggcgcctat ccgatccttc agtcgggctc gggtgcgacg
     3001 agggaccgct ggatccctgc cgtgcgcaag ggcgaggcgg tcgccgcgtt cgcgctgacc
     3061 gagccggagc acggctccga tgccgccgcg ctcgagctcg ccgcggcgcc cgacggcgac
     3121 ggttggcgcc tcagcggcga gaagcgctgg atctccaacg ccccggatgc cgacgtctac
     3181 accgtgttcg cgcgcaccac gccggacgcc ggcgcgaagg gcgtgacggc cttcgccgtc
     3241 tccggggacg ccgagggtct gtcaggcgag tccctcgacc tgctggctcc acacgcgatc
     3301 ggcgcgctgc gcttcgacgg ggtgcgcgtc gggcccgagg acgtcctcgg tgaggtggac
     3361 ggcgggttcc gcgtcgcgat gcggacgctc gacctcttcc gcccgagcgt gggtgccttc
     3421 gcggtgggga tggcgcaggc ggcgctcgac ctcgcggtgg cgcacgccgg tgagcggcga
     3481 tcgatgggca agctcctgcg cgagcaccag gcggtctcgc agaagctcgg ggagatggcg
     3541 gcccggctgg aggcggcacg gatgctggtc tacgcggcgg cgtccgccta cgatcgcggc
     3601 gagcgcgtca cgaagatcgc ggcgatggcg aagctggtcg cgacggagac ggcgcagtgg
     3661 gtggtcgatg aggccgtgca gatccacgga gcggtggcgc tcgaacgggg tcaccccctc
     3721 gagcacctgt accgcgacgt ccgcgcgccg cggatctacg aagggacgag cgagatccaa
     3781 cgggagatca tcgctcggga gctctatcgg agggaggagt agatggacct gtacatgatc
     3841 gtgctccgga tcgttcacat cctggccggc gtcttctggg tcggctcggc ggcgatgttc
     3901 ttcatgtacg tgcagccgac cgcgaaggcg ctcggtcccg ctggcgcgcc attcatgacg
     3961 cacctcgtcc agcagaagcg cgtcccggtc gtcctgctgt ggtgcgccgg aacgacgatc
     4021 acggcgggac tgctcctcta ctggcgcacc tcgggcggct tcgacaccga ctggatcacg
     4081 agcgagacgg ggatggcgtt cacgatcggc ggcctcgcgg cgatcgtcgc gttcatgctc
     4141 ggcctcttca tcgcgaagcc cgccgccgat cgcatgggag cggtggctgc ccaagcggcc
     4201 gcggccggcg gcccgccctc gcaggagcag gcggcgcaga tcgccgcgtc ccaggcgacg
     4261 ctgcggaaga tcggcatcac gaacctgacg ctcctgaccc tcgcggtgat cctgatggcg
     4321 gtcgcgcgct acctgtagtc gctcaagcgg gcacgacggc gacgcagacg agctcgacct
     4381 tcgcgccggg ctcgaacagg gcgctgacct cgaacagcga caccgccggg tagtgccggc
     4441 cgaagtgacg ctgccagacc tcgccgatgg ccggcagcga ggtccggtac tccatgagcg
     4501 aggtcacgaa gatctggatc gagacgaggt gctccgcccg agcccccgcc gcgagcagcg
     4561 cttcgatgac gttggagcac gcctggtcga gctgctcgac cacggtcgat ccggcgagcc
     4621 gcccgtcctg ggccgcgccg atctggccgc ccaggtagat cgtccgccca ggggtcggga
     4681 cgacggcgtg ggagaacccg ctgggccgag gcaacgtcga cgggttcagc atgcggtgcg
     4741 gagagctcgc cttcatcgtc ccctccaggt cggtcggcgg ccttcggtga aggcccggta
     4801 gaactcgcgg tggtcctcgc tcgacatcaa gagcgcctgc gtcatcgcct ccagctcgag
     4861 cgcccccgcg agcggcatgt cctgctcgcg ggcgaggagg agcttcgtgg cggcgtgggc
     4921 gagagcaggt ccctcggcca gccgccgcgc cagcgacgtc gcgcgctcca ccagctcggc
     4981 gtcctcgacc acctcacgcg cgagcccgat ctcgaccgcg cgctccgcgg ggacgtcctc
     5041 gccgagcagc aggagctcgg tggcgcgacc gagcccgatg acccgcggga gcaggtacgc
     5101 cgagcccatg tcggcgccgg ccagtccgac gcgtgtgaag aggaaggcga acgacgcgct
     5161 tcgggcgagg atcctgacgt cggcggcgag cgcgatcacg gatccagccc ccgcggcgac
     5221 gccgttcacg gccgccacga tcggctgcgg cagctcgcgc atgtgctgga cgaccgcccc
     5281 ggtcatccgg gtgaactcca gcagctcctt cgcgccggcc ccctggagcg cgccgatgat
     5341 gtcgtcgcga tccccacccg cgcagaaccc acgcccctcc ccggtcagga cgacgacgcg
     5401 gacgtcgtct cgatgcggca gctcggcgag gaggtcgcgg aagtcggcgt aggaatcgaa
     5461 ggtcagcgcg ttcagccgtt cgggtcgatc gaacgtgatc gtcccgatgc cgtcggcgac
     5521 ctcgaaccgg aggtgcttcc agtcgtcggt gaacggtgcc gacgcgcggt gcgggctcac
     5581 tactcgaacg cgtcgagccc ggtgagggcc ttgccgagga tcagcgtgtg gacctcgttc
     5641 gtcccctcgt acgtgacgac cgactcgagg ttgttcatgt ggcggatcac cgggtactcg
     5701 agggtcacgc cgttcgctcc gaggatcccg cgcgcctcgc ggcagatcgc gagggcctcg
     5761 cggacgttgt tgagcttgcc gaagctgacg tgctccgggg ccgtccggcc ctcgtccttc
     5821 atgcgaccga ggtgaagcgc gagcaggagg cccttgtgga gctcgaccgc catgtcgacg
     5881 agccgccgct gcgtgagctg gaaggcgccg atcggcttgt cgaactggat ccgcgtcttc
     5941 gcgtagtcga cggcggtctc gacgcacgcg cgcgcggcgc ccatggagcc ccagacgatc
     6001 ccgtagcggg cctcgttcag gcatgacagc ggccccttca tcccgcgcac cccggggagc
     6061 accgcgtcgc ccggcagccg gacgtcgtcg aagatcagct cggaggtcac ggaggcgcgc
     6121 agcgacagct tccgctcgat gtccttcgtg gtgaagccgt cgacgccgcg cggcaccagg
     6181 aagccgcgga tgccgtcgtc ggtctgggcc cagacgacgg cgatgtcggc gacgccgccg
     6241 ttcgtgatcc acatcttcgt tccggagagg atccagtcat ccccgtcgcg tcgggcgaac
     6301 gtccgcatgc cggcggggtt cgatccgtaa tccggctcgg tcagcccgaa gcacccgatc
     6361 gcctcgccgg cggccatccg cggcagccac tcctgcttct gctcctctga gccgtaggcc
     6421 cagatcggga acatcgcgag cgatccctgg accgagacga gcgagcggat gccgctgtcc
     6481 ccggcctcga gctcgaggca cgcgagcccg tacatgacgg cggacgcacc ggcggtgccg
     6541 tagccctcga ggtgcatccc gagcaggccc atcgagccga gctccttcgc gagctgctgc
     6601 gacgggatct ctccgcgctc gaaccactcg gcgatctccg gcaggacccg gctgcccacc
     6661 cactcgcgga ccgtgttgcg gacggccttc tcctcgtccg acagcagatg gtcgagcgcg
     6721 aggaggtccc gggggtccac ctggggtcgg ctggagacct gcgtcatgct gtccagcgta
     6781 gcggattcgg aaggcgaacc tcctgtgcag ctgcacaaag ggatcgaggt gtgccgtcac
     6841 gaatccaccg cgcgacgacc gaccctggag gagccggtga tgaagcgcac gatcccgatc
     6901 ctgctggccg ccttcgtgac gctcgccgtt ctccccggac ctgccgcggg cagtgccacg
     6961 atcaccttct cggggagcag gcaggtccgc ggcgatctcg gtgacatggg tgcggacccg
     7021 ctcgacggag ctcgcacggc acttgacgcg ttcgccgacg agctcggcgt ggagccctcg
     7081 cgcttcagcc tcgacgaggt acgccggtcg atcgttggga tccatgtgcg cggcgtcgag
     7141 cgccggggcg gggtccccgt cgagaacacc gcggtcgcgg tccacgccgt cgacggccgg
     7201 atcgtgcaga tcgacgccca tggctcggat ctgcgcgggt ccgccgccgg tgtcgccatc
     7261 tctcggcatg ccgccgtcac ggccgcgctt gcgcacgccg gtgtcgcgcg gacgctgctc
     7321 cccccgcgca cgcagcgtct gctggttgcc tccgaagggc ggctcgtcga cgtctggcgg
     7381 gtgccggtgc tctccctcga gcccgcggtc gcgctgacgg tcgacgtgga cgcggcgagc
     7441 ggcaggatcg tgcgcgcggt ggatgatcgc ctccacgcgg aagggcaagc gaccgtcttc
     7501 gacccgagcc ccaccgtgac cctgcgggac acgacgctcc gccagccggg ggtggaccgg
     7561 ctcggcgtcg ataccgacct tcccgacgag cgcctcaacc gcgcgctccg gctcctgccg
     7621 gtccgcgacg tcgcctcggc gacgcaggat ctcctcgccg ggcggctcca cgggccgtac
     7681 gtggacgtgt acggggcccc cggcctcgat ccgttcggtg ggtggttcta ccagcgacac
     7741 gacccgaggt tcgagacgct gatggcctac acgcacatcg accggatcca gcggtacttc
     7801 cagtcgctcg gcttcgtcgg ctccgccgcg atcaacgcgg agccgcagtg ggtcatcacg
     7861 catcgggtcg agggatacga caacagcttc ttccagccgg cgaacgacct gatcctcttc
     7921 ggcgccggcg gcgtggacga cgcggaggac gccgaggtgg tgctccacga gctcggtcat
     7981 gccatccact acgaccaggt tccgaactgg ggcgggcacc atcaggccgg tgcgatgggc
     8041 gaggggttcg gcgacttcct cgccgccgcg tactacgcgg gatcgatcag cggcggattc
     8101 ggggacacgt gcgtggccga ctgggatgcg acctcgtact ccccggccaa cccgccgtgc
     8161 ctgcgaaggg tcgataccgc gaagaggttc ccgcagaaca tgacgaacaa ctccgtgcac
     8221 gccgacggcg agatctggtc ggggttcttc tgggacctgc gccagctcct gaagtgcccc
     8281 gccgagccgc ccgaggacga ccccgagtgg gagaccgatc cggagtgcaa gccgccgcgg
     8341 aacgactcgc agatccagag cgaccgggtc ctgaagctgc tcctgacctc gcacgagttc
     8401 ctcacgaacg gcgcgacctt cgggcgagcc gtcgcagcgc tccggaccgc agccgacgcg
     8461 atcggacgcc cggagtggaa ggcgctcgtg gatcaggccg cgaagggccg agggctgccg
     8521 ctcaacccgt agcgctcgaa ccacagc
//
LOCUS       DATLGG010000080         7049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_52492, whole genome shotgun sequence.
ACCESSION   DATLGG010000080 DATLGG010000000
VERSION     DATLGG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52492"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..400)
                     /locus_tag="VM638_02210"
     CDS             complement(65..400)
                     /locus_tag="VM638_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11278.1"
                     /translation="MNEMTTIRATCPSCGEVEMDAQAILLSVRDDDGEGTYSFTCPVC
                     RDTVEKPADRKVVMLLLSAGVDVTRGEQVDVPTEEVPADLPPLTADDLIDFHFMLERD
                     EEWFARLAG"
     gene            complement(561..1508)
                     /locus_tag="VM638_02215"
     CDS             complement(561..1508)
                     /locus_tag="VM638_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVM11279.1"
                     /translation="MPPGRTAERLRRLLALVPYVVRNPGTSVAELAELFGTTREALTS
                     DLNLLLFTGLPPYSPGDLIEVEIEEGRVWIRMADYFSRPLRLTRAEALSLYLRGAELS
                     GAPGLEEAQALRSALEKLRDALGPETLGELTAEVEGEDADGAGPLPAIRAAVAARERI
                     EIDHYSIGRDELTTRRIDPEEVFPALGRWYVVAWCHMVDGERLFRVDRIREARPTGER
                     FEPRGLLGQGRPLYSPSQQDRMVRLRLGPRARWVAEYYATDDEREVDGRLEISLPTKD
                     LGWVAKLVLRLGGDVEVAEPPELGQLARDVAQATLALYR"
     gene            complement(1495..2445)
                     /locus_tag="VM638_02220"
     CDS             complement(1495..2445)
                     /locus_tag="VM638_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVM11280.1"
                     /translation="MQPLERLLNLVVLLLETRRPLTFEEIRDGLAAYDQEDVASAKRQ
                     FERDKDQLRDIGIPLEVAPVDVWESAEGYRIPKERYYLPQIELSPEEAAALFVAARGS
                     RRGDDAGQALVKLLVGSGSGLLGAAEAPGTAGPDASAPHLPAAAEAASRRRRVAFTYR
                     SARGEERERSVDAWGLVFRRGSWYLVGRDVAQDEPRAFRLSRIRSSLRDTGAGEAPPE
                     GFRAAEHIVAGPWGVGEPEGTARVAFSPKVAWWAVPAIPGAQVASTRPDGWTEADVPA
                     APGEEFVSWVLSFGPDAVVVAPAELRRAVVERLEAFGAAG"
     gene            2479..3570
                     /locus_tag="VM638_02225"
     CDS             2479..3570
                     /locus_tag="VM638_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HVM11281.1"
                     /translation="MIRLRRATVSAVKGSRPGLLELAVEVDGRTERALCYPALTGPVV
                     EGDVVLLNTTAVDLGLGTGGTHFVVAGPNERDASPGHVMKLRYTPLQAVVPTVEEQAS
                     PHHEAMARATSLDGLPVAWVPLHSMVAAAAAGARTAGAARVAYVMTDGAALPAWLSDQ
                     VAALRSTGIIEAVVTVGQALGGDLEAVNVFSGLLAARMVAGADAVIVADGPGKVGTAT
                     PWGASELASAMALNAAGTLGGRPIAALRLNFSDLSYRHHGISPHSLRVLAQVALVPLH
                     VAIPVLPDERRDPVWTALREARLEERHQLVEVAGQPGVDLLRDRGIPMQTMGRTEAEE
                     PEFFLAAGAAGVLAGRMAAGAARWRQERA"
     gene            3567..4235
                     /locus_tag="VM638_02230"
     CDS             3567..4235
                     /locus_tag="VM638_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11282.1"
                     /translation="MRTLEVAFDASGAAEAGSRGRVVAVVDVVDAATSAEAAVAMGAL
                     DVFGAAPAGAAPPVPVNPAAVATRAATVALRRGTDVVVVAEPRVGAADERLGRVAPVT
                     AGLEAAGVSYEVVANQGAEVAGLAALTGRVVVVVSTTGGTAFDAALAAGAPGACFVTT
                     ARVAGMTGWEVTRMGALRAIDLADRHGGALSLVAASANSTDDCLAAFEVARAVIDQRF
                     LRPD"
     gene            complement(4242..5600)
                     /gene="pafA"
                     /locus_tag="VM638_02235"
     CDS             complement(4242..5600)
                     /gene="pafA"
                     /locus_tag="VM638_02235"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HVM11283.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVTDLVAHDKAGERILEGLLGAAQMRLHEEGI
                     SGDVFLFKNNTDSAGNSYGCHENYLVARQGEFARMAEILIPFFVTRQIYAGAGKVLHG
                     PRGAHYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEF
                     TMLLKVGVTDLVLRMVEEHNSMRDLTLENPIRAIREISHDITCRKKVRLANGRELSAL
                     DIQTEYFERAKRFADAKGLDPLGLRVLAEWGAALEALRSQDLEPLVRKVDWVAKLNLI
                     DRYREKHALPLGHPRVALMDLAYHDVNRDRGLYYLLERRGEMERVVEETQVQRAMKDP
                     PQTTRARLRGEFIRRAKQRRRDYTVDWVHLKLNDQAQRTVLCKDPFRSQDDRVEQLMA
                     HM"
     gene            complement(5617..6489)
                     /locus_tag="VM638_02240"
     CDS             complement(5617..6489)
                     /locus_tag="VM638_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11284.1"
                     /translation="MTVRWVREGACLPPARPDDADAVRAWRPWVLEDEGGLRMWYAGH
                     DGTTWRILEAAGDGSAWERLGVVVDAGLAGETDDFGVQDPCVIRTRGGYLMAYAGSDG
                     EVTRLHMASSMDGHAWSPLGTFLQRGEEDHQGATHPSVLTVGTRWWLFFSAYEGTGRE
                     RRSSILAAISDNGASWDRVGPVLVPEGEEVAAAHPCVIDHELELHMFYALDDGQRTGI
                     ALATSKNGIDWDRRGSVLPPEGEGPDALAVDSPCVVRLADGSIRMWYVGLPLEDAQHA
                     YRICSARFEGELSR"
     gene            complement(6486..>7049)
                     /gene="prcA"
                     /locus_tag="VM638_02245"
     CDS             complement(6486..>7049)
                     /gene="prcA"
                     /locus_tag="VM638_02245"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HVM11285.1"
                     /translation="ADNPSTHLHKISEVYDRIAFAGVGKYSEFENLRIAGVRMADVRG
                     YSYGREDVTVKAVANAYSQVLSDIFTREMKPYEIEILVAQVGEGAEENELFHILFDGS
                     VTDQSGFVAMGGESEILGNALSGTYRESMTLEEAVGTGVQALSTVNGGTLEPARVEAA
                     ALDRTRRGRRKFRRLGVEDIERILSAG"
BASE COUNT          973 a         2578 c         2479 g         1019 t
ORIGIN      
        1 gggcgatggt gagcatgcgg cgctcaggat accaggggcg ataccgctgg gttccgcagg
       61 aggatcagcc cgcgaggcgg gcgaaccact cctcgtcgcg ctccagcatg aagtggaagt
      121 cgatgaggtc gtcggccgtc agcggcggaa ggtccgcggg aacctcctcg gtcggcacgt
      181 cgacctgctc gccgcgggtc acatcgacgc cggcggacag gaggagcatg accaccttgc
      241 gatcggccgg cttctcgacc gtgtcacggc agaccgggca ggtgaacgag tacgtgccct
      301 cgccgtcgtc gtcccggacg ctcagcagga tcgcctgcgc gtccatctca acctcaccgc
      361 agctggggca cgtggcccgg atcgtggtca tctcgttcat gtctatcggc tccctctcgg
      421 ctcccttgag gaacgcgatc cgtcagccca gaaactccca cgatgtccac ttccgtgtat
      481 atggcccgct ctgatcatct tcggcatggc tcgttcgggt cattcccgaa gtggtcctcg
      541 aggaaacgcc cgaaccggtc ctaccggtag agggccagcg tcgcctgcgc gacgtcgcgc
      601 gcgagctgac cgagctccgg cggctcggcg acctcgacgt cgccaccgag ccgcaggacg
      661 agcttcgcca cccatcccag gtccttcgtc ggaagggaga tctcgaggcg gccgtcgacc
      721 tcccgctcgt cgtccgtggc gtagtactcg gcgacccacc gcgcgcgagg cccgagccgc
      781 agacgcacca tccgatcctg ctgcgacggc gagtacagcg gcctgccctg tccgaggagc
      841 ccccgcggct cgaaccgctc ccccgtcggg cgtgcttccc ggatccggtc gacgcggaac
      901 agacgctcgc cgtcgaccat gtggcaccag gccaccacgt accagcggcc gagcgccggg
      961 aagacctcct cggggtcgat ccgcctggtc gtcagctcgt cgcgaccgat cgagtagtga
     1021 tcgatctcga tgcgctcccg cgcggccacg gcggcgcgga tcgccggcag ggggccggcg
     1081 ccgtccgcgt cctcgccctc gacctccgcg gtcagctcgc ccagggtctc cgggccgagc
     1141 gcgtcccgca gcttctcgag ggccgaacgc agcgcctgcg cctcctccag gcccggcgcc
     1201 ccgctcagct cggcgccccg caggtagagc gagagcgcct ccgcccgcgt cagccgaagc
     1261 ggccgggaga agtagtcggc catcctgatc cagacgcggc cctcctcgat ctcgacctcg
     1321 atcaggtctc ccggactgta cggggggagt ccggtgaaca ggagcaggtt gagatccgag
     1381 gtcagcgcct ccctggtcgt tccgaagagc tccgcgagct cggcgaccga tgttccgggg
     1441 ttgcgaacga cgtagggaac cagcgcgagc agccggcgca ggcgctcggc cgtcctaccc
     1501 ggcggcaccg aacgcctcca gccgctcgac gaccgcacgc cgcagctcgg cgggcgcgac
     1561 caccaccgcg tccgggccga acgagaggac ccacgacacg aactcctcgc cgggcgccgc
     1621 cgggacgtcg gcctcggtcc acccgtccgg ccgggtcgag gcgacctgcg cgcccgggat
     1681 cgcgggcacg gcccaccagg cgaccttcgg actgaacgcg acgcgggccg tcccctccgg
     1741 ctcgccgacg ccccacggcc ccgccacgat gtgctcggcg gcgcggaacc cttcgggggg
     1801 agcctctccc gctccggtgt cccgcaggct cgaccggatc cgcgacaggc ggaacgcccg
     1861 tggttcgtcc tgggcgacgt cccgcccgac gaggtaccag cttccccgcc ggaagacgag
     1921 cccccaggcg tcgaccgaac gctcccgctc ctcgccgcgc gccgaacggt aggtgaaggc
     1981 gacccgtcga cgccgcgatg ccgcctcggc cgcggccggc aggtgcggcg ccgacgcatc
     2041 cggcccggcg gtccccggag cctcggccgc gccgagcagc ccggaccccg agccgacgag
     2101 gagcttcacg agcgcctgac cggcgtcatc cccgcggcgg gatccacgcg ccgcgacgaa
     2161 gagcgccgcg gcctcctcgg gggacagctc gatctgcggg aggtagtagc gctccttcgg
     2221 gatgcggtag ccctcggccg actcccacac gtcgaccgga gcgacctcga gcgggatgcc
     2281 gatgtcgcgc agctgatcct tgtcgcgctc gaactgccgc ttcgcgctgg cgacatcctc
     2341 ctgatcgtag gccgccagac cgtcgcggat ctcctcgaag gtgagcgggc gccgtgtctc
     2401 gagcagcagg acgaccaggt tcagcaggcg ctcgaggggc tgcatcactg gggatgatag
     2461 tccgactacg atgggccggt gatcaggctc cgtcgcgcca cggtctccgc ggtcaaggga
     2521 tcgcggccag gactgctcga gctcgcggtc gaggtcgacg gccggacgga acgtgccctc
     2581 tgctacccgg ccctgaccgg accggtcgtc gaaggtgacg tggtgctcct caacaccacc
     2641 gccgtcgacc tcggcctcgg gacgggcgga acccacttcg tcgtcgccgg gccgaacgag
     2701 cgggacgcgt cgcccgggca cgtgatgaag ctgcgctaca cccccctgca ggcggtcgtc
     2761 ccgacggtcg aggagcaggc cagcccccac cacgaggcga tggctcgcgc aacgtcgctc
     2821 gacgggctgc cggtcgcgtg ggtccccctc cattcgatgg tcgccgccgc cgcggcgggt
     2881 gcccgcacgg ccggggcggc ccgcgtcgcg tacgtgatga cggacggcgc ggcgctgccc
     2941 gcgtggctgt cggaccaggt cgccgccctg cgctcgaccg ggatcatcga ggccgtcgtc
     3001 accgtcggtc aggcgctcgg aggggacctg gaagcggtga acgtcttctc gggtctcctc
     3061 gccgcgcgga tggtcgccgg cgcggatgcg gtgatcgtcg ccgacgggcc gggcaaggtc
     3121 ggcacggcca caccgtgggg ggcgagcgag ctcgcatcgg cgatggcgct gaacgccgcc
     3181 gggaccctcg gtgggcggcc gatcgcggcc ctgcgtctga acttctcgga cctctcgtac
     3241 cggcaccacg gcatctctcc tcattcgctc cgcgtcctcg cccaggtcgc cctcgtcccg
     3301 ctccacgtgg cgatcccggt ccttcccgac gaacggcgag acccggtgtg gacggcgctg
     3361 cgcgaggcgc ggctggagga gcggcaccag ctcgtcgagg tcgccggcca gcccggggtc
     3421 gacctcctcc gggaccgagg gatcccgatg cagacgatgg gccggacgga ggcggaagag
     3481 ccggagttct tcctcgcggc cggtgcggcc ggcgtcctcg ccggacggat ggcggccggc
     3541 gcggcccggt ggaggcagga gcgcgcgtga ggaccctcga ggtggcgttc gatgcgagcg
     3601 gggccgccga ggccgggtcg cgcggacgcg tcgtggccgt ggtcgatgtg gtcgacgcgg
     3661 cgacgagcgc cgaggccgcc gtcgcgatgg gcgcgctcga tgtcttcggg gccgctcccg
     3721 caggcgccgc gccccccgtc ccggtcaacc ccgccgccgt cgccacccga gccgcgaccg
     3781 tcgccctgcg tcgaggcacc gacgtggtgg tcgtcgccga gccccgcgtc ggggcggccg
     3841 acgagcgcct cggccgcgtc gccccggtga cggccggact cgaggcggcg ggcgtctcct
     3901 atgaggtcgt cgccaaccag ggtgcggagg tggccggact cgccgcgctc accggacggg
     3961 tcgtggtcgt ggtctccacc accggcggga cggcgttcga cgcggcgctc gccgccgggg
     4021 cgcccggcgc ctgcttcgtg acgaccgctc gggtagccgg gatgaccggg tgggaggtca
     4081 cgcgcatggg ggcgcttcgc gcgatcgacc tcgcggaccg tcacggcggg gcgttgagcc
     4141 tcgtcgcggc gagcgcgaac tccaccgacg actgcctcgc ggccttcgag gtcgcgcgcg
     4201 cggtgatcga ccaacgcttc ctgcgtcccg actgagcgca gctacatgtg cgccatcagc
     4261 tgctcgaccc ggtcgtcctg cgagcggaac gggtccttgc acaggaccgt ccgctgcgcc
     4321 tggtcgttca gcttcaggtg cacccagtcg accgtgtagt cgcgccgccg ctgcttcgcc
     4381 cgacggatga actctccgcg gagccgcgcc cgggtcgtct gcggaggatc cttcatcgcc
     4441 cgctggacct gcgtctcctc gaccacgcgc tccatctcgc cgcgccgctc gagcaggtag
     4501 tagagccccc ggtcccggtt cacgtcgtgg taggcgaggt ccatgagggc cacgcgcgga
     4561 tgccccagcg gaagtgcgtg cttctcgcgg tagcgatcga tcaggttgag cttcgcgacc
     4621 caatcgacct tgcggacgag aggctcgaga tcctggctgc ggagcgcctc gagggccgcc
     4681 ccccactcgg cgagcacccg gaggccgagc gggtcgagtc ccttcgcgtc ggcgaagcgc
     4741 ttggcgcgct cgaagtactc cgtctggatg tcgagagcgg agagctcccg cccgttcgcg
     4801 aggcggacct tcttgcggca ggtgatgtcg tggctgatct ctcggatcgc gcggatcggg
     4861 ttctccaggg tcaggtcgcg catcgagttg tgctcctcca ccatccgcag cacgaggtcc
     4921 gtcacgccga ccttcagcag catcgtgaac tcgctcatgt tcgagtcgcc gacgatcacg
     4981 tgcaggcgcc ggtaccgctc cgcgtccgcg tgtggctcgt cgcgggtgtt gatgatcggc
     5041 cgagagcgcg tcgtcgcgct cgagacgccc tcccagatgt gctcggcgcg ctgcgagagg
     5101 cagtagtgag ccccccgagg gccgtgcagg accttgccgg ctcccgcgta gatctggcgc
     5161 gtgacgaaga acgggatcag gatctcggcc atccgcgcga actcgccctg gcgcgcgacg
     5221 aggtagttct cgtgacagcc gtacgagttg ccggcggagt cggtgttgtt cttgaagagg
     5281 aagacgtcgc cggagatccc ctcttcgtgc aggcgcatct gcgccgcgcc gaggagccct
     5341 tcgaggatcc gctcccccgc cttgtcgtga gcgacgaggt ccgtcaccga gtcgcactcc
     5401 ggcgtggcgt actccggatg cgagccgacg tcgaggtaga ggcgcgcgcc gttctcgagg
     5461 aagacgttgg acgagcgccc ccagtgcacg acccgtcgga agaggtaccg ggcgacctcg
     5521 tccggggaga ggcgccgctg cccccggaac gtgcaggtca cgccgtactc gttctccagg
     5581 ccgaagatcc gtcgttccac ggggtcagtg tagggactac cgcgacagct cgccctcgaa
     5641 acgggcggag cagatccggt aggcgtgctg ggcgtcctcc agcggcaggc ccacgtacca
     5701 catgcggatc gagccgtcag cgagacggac cacgcacggc gagtcgaccg cgagggcgtc
     5761 ggggccctca ccctccggcg ggaggacgct cccgcgccgg tcccaatcga tgccgttctt
     5821 cgacgtggcg agcgcgatgc cggtccgctg gccgtcgtcg agcgcgtaga acatgtgcag
     5881 ctcgagctcg tggtcgatca cacagggatg agccgccgcg acctcttcgc cctcggggac
     5941 gaggacgggt ccgacgcggt cccaggaggc gccgttgtcc gagatcgccg ccaggatcga
     6001 ggaccgccgc tcccggcccg ttccttcgta cgccgagaag aagagccacc accgggtccc
     6061 gaccgtcagc acgctcgggt gggtggcgcc ctggtggtcc tcctcgcctc gctggaggaa
     6121 cgttccgagc ggggaccacg cgtggccgtc catcgaggag gccatgtgca ggcgggtgac
     6181 ctccccgtcg gagccggcgt aggccatcag gtagccgccg cgcgtgcgga tcacgcaggg
     6241 gtcctggaca ccgaagtcgt cggtctcacc ggcgaggccc gcgtcgacga cgacgccgag
     6301 ccgctcccac gccgagccgt ccccggccgc ctcgaggatc cgccacgtgg tgccgtcgtg
     6361 gcccgcgtac cacatgcgga gaccgccctc gtcctcgagg acccatggac gccaggcgcg
     6421 gaccgcgtcg gcgtcgtccg gccgggccgg cgggagacac gcgccctccc gcacccagcg
     6481 cacggtcatc ccgccgagag gatccgctcg atgtcctcga cgcccaggcg ccggaacttg
     6541 cgccggcccc ggcgcgtgcg atcgagggcg gcggcctcga cacgggcggg ctccagggtc
     6601 ccgccgttca cggtcgagag cgcctggacg cccgtgccga ccgcttcctc cagcgtcatg
     6661 ctctcgcggt aggtgccgct cagcgcgttg ccgaggatct ccgactcgcc ccccatcgcg
     6721 acgaagcccg actggtcggt gacgctgccg tcgaacagga tgtggaagag ctcgttctcc
     6781 tccgcgccct ctccgacctg cgcgacgagg atctcgatct cgtagggctt catctccctg
     6841 gtgaagatgt ccgagaggac ctgcgagtac gcgttcgcca ccgccttcac cgtcacgtcc
     6901 tcgcgtccgt aggaataacc gcggacgtcg gccatccgga caccggcgat ccgcaggttc
     6961 tcgaactcgc tgtacttccc gacaccggcg aaggcgatcc ggtcgtagac ctcggagatc
     7021 ttgtgcagat gggtcgaggg gttgtccgc
//
LOCUS       DATLGG010000081         3788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_146018, whole genome shotgun sequence.
ACCESSION   DATLGG010000081 DATLGG010000000
VERSION     DATLGG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146018"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VM638_02250"
     CDS             complement(<1..510)
                     /locus_tag="VM638_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11286.1"
                     /translation="MLDRRRRRDRNRRIGTAVVAVVVAAVGIGGVVRAFSPDLETRPA
                     HQLGEPFLGAWASVDEAGFEETSQTMTIRAGKDGGFRVTMHDEGSFDCPRPHGEPPNQ
                     VYTQLPKTLTGAGQLKDPTTLVVPSPVLTCEDGDAPATSPLDEAGGSYTLTFDPVTDR
                     LYDNLGWVWN"
     gene            complement(575..1054)
                     /locus_tag="VM638_02255"
     CDS             complement(575..1054)
                     /locus_tag="VM638_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HVM11287.1"
                     /translation="MTTPRFEDFFEAEHVRLARALYLLTGSAAEADELSQEAMVRVYE
                     RWDRVRQMDSPQGYLFRTAMNLHRSRVRWLASRARHILQPSASPDPAEVVQNRDSVAR
                     ALASLPTGQRGAVVLVEWLGMGAQEAATALGIKPVSVRARLSRAKAALRETLEDQDV"
     gene            complement(1170..2162)
                     /locus_tag="VM638_02260"
     CDS             complement(1170..2162)
                     /locus_tag="VM638_02260"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVM11288.1"
                     /translation="MRSDGFGEGGGMSATVMTAEGTWSGTVGTADGARDLQVDDQFAI
                     ASITKSVVAAQVMSMAEAGELGLEDPVADHLPRDLGFDTNGATIRDLLGHRSGIADGY
                     DVLRGPVQGDPRRAWKPADVLPRVPSTREAIGSFRYAETNYLLLGLVIEHLRGRPLAE
                     VLRRGVLAVDGMERLIYQPDEKPTEPIALPRGTSSTDLEDLGGHLPSLAAVTAFSGSG
                     VMASDAPSLARWWRAFCAGEIVSQASLTEMSAFDPDASVGDGGYGLGLFNPAYGHAPA
                     VGHQGELFGYMSWAACLPEDGAVIVVLSNREVHAMSEELFFRPLRPFVDVLRSG"
     gene            complement(2450..>3788)
                     /locus_tag="VM638_02265"
     CDS             complement(2450..>3788)
                     /locus_tag="VM638_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11289.1"
                     /translation="VWERKGSKPDTLSGGMWPQANLEEVKQAQERADAGDSDYTWQLD
                     AQLAAEDHDGTWNRLYNGEVELVDRFLREELGWEAYMQNLHVGGADHSLTDQRFLRCA
                     PGRTNPLYPPQPDSRNPGERCAPTLDELTYESVSLDLVQPGRKGRGGIWVVSNWRTTT
                     FTQADPAVAEAQATRRLEEFLAARVAGRGAEGYVDVYADWIEPEVPLLYATSSGSSYE
                     RSEFERVAGPAWPYGGYIEFRIRLFAEDGSVVEQEIHSHWDAGVSAGTTGGLALGART
                     TENGRPVPMPFAHFDGEVTYSSPSQNPMVIGDERDPSGLLDFTDPAVFWSECGRGAVP
                     ADAAAFAQAVIADRNFESTSPVAARVGGAKAVTIDVELSPTGRVCTAYRSDAQRWIHA
                     LEPGKRLRLFLVDLPKGMSMRTLAITVMAPKGSFEEALEEADPVIDSVRFHTS"
BASE COUNT          577 a         1304 c         1289 g          618 t
ORIGIN      
        1 gttccacacc catccgaggt tgtcgtagag cctgtcggtg acggggtcga aggtcagcgt
       61 gtagctgccg cccgcttcgt ccagggggga ggtggcaggg gcatctccgt cttcgcatgt
      121 caggacgggc gagggcacca ccagggttgt cgggtccttg agctgcccgg cgccggtcag
      181 cgtcttcggg agctgcgtat agacctggtt gggcggttcc ccgtgggggc gcgggcagtc
      241 gaaagaaccc tcgtcgtgca tcgtgacgcg gaagccgcca tcctttcctg cgcggatcgt
      301 catggtctga gaggtctctt cgaagccggc ctcgtcgacg gaggcccatg ctcccaggaa
      361 gggttccccg agctgatggg ccggccgggt ttcgaggtcc ggggagaagg cccggacgac
      421 cccgccgatg ccgactgccg ccaccaccac ggccaccacc gcggtcccga tccttcggtt
      481 gcggtcccgc cggcgtcggc ggtcgagcac ccggtcgaac gaatcggcag gaggctcgta
      541 gcgcgaggct cgccgctcga ggaggtcgtg aagctcagac atcttgatcc tccagggtct
      601 ctcggagggc cgctttggcg cgggacagcc ttgcccggac cgaaacgggc ttgatcccca
      661 gggccgtggc ggcctcctgc gcgcccatcc ccagccactc gaccagcacc acggccccgc
      721 gctggccggt cggaagcgag gcgagcgccc tggcgacgct gtcccggttc tggaccacct
      781 cggcgggatc gggggacgct gagggctgga ggatgtgccg cgcccggctg gccagccatc
      841 ggacccggct tcggtgcagg ttcatcgcgg tcctgaacag gtatccctgc ggggagtcca
      901 tctgccggac ccggtcccac cgctcgtaca cccgcaccat ggcctcctgg ctgagctcgt
      961 cggcctcggc ggcgctaccg gtcaggaggt agagggcgcg agccagtcgg acgtgctccg
     1021 cctcgaagaa gtcctcgaag cggggggtgg tcaccggggt gacctcctct acctcctctg
     1081 ctggtccggt cgccgagatc tccatcatct cggagcgtga acgcaacagg gggtcgatcc
     1141 gttgcgcccg aggtgaggga tcttttcggt cagcctgagc gcaagacgtc gacgaagggc
     1201 cggaggggcc tgaagaacag ctcctcggac atcgcatgga cctcgcggtt gctcagaacg
     1261 acgatgaccg ctccgtcctc cggcaggcac gcagcccacg acatgtagcc gaacagctct
     1321 ccctggtgcc ccacggctgg ggcgtgcccg taggccggat tgaacagccc gagtccgtac
     1381 cccccatccc cgaccgacgc gtccggatcg aacgcggaca tctcggtgag ggaagcctgg
     1441 gagacgatct cgccggcgca gaacgcgcgc caccaccgcg cgagggacgg cgcatccgag
     1501 gccatgactc ccgagccgct gaaggcagtg acggcggcga gcgatgggag gtggccgccc
     1561 agatcctcga gatcggtgct ggatgtgccc cgcggcagcg cgatcggctc ggtcggcttc
     1621 tcatcgggtt gatagatgag ccgctccatc ccgtcgacgg cgaggacgcc gcgtcgcagc
     1681 acttcggcca gcgggcgtcc ccgaaggtgc tcgatcacta gaccgagcag cagatagttc
     1741 gtttccgcgt atcggaacga gccgatagcc tctcgcgtgc tcggcacccg cggcagcacg
     1801 tcggccggct tccacgcgcg ccgcgggtca ccctgtacgg gcccgcggag cacgtcgtat
     1861 ccgtctgcta tgccgctgcg atgacccagc agatcacgga tggtcgcccc gttggtgtcg
     1921 aacccgagat cccgaggaag atgatcggcg accggatctt cgagacccag ctcgccggcc
     1981 tcagccatcg acatcacctg ggccgcgacc accgacttcg tgatgctcgc gatcgcgaac
     2041 tggtcgtcga cctgcaggtc tcgcgcaccg tccgccgttc cgacggtccc gctccaggtg
     2101 ccctcggcgg tcatcaccgt ggccgacatc ccgccacctt caccgaagcc gtcgcttcgc
     2161 accaggtcat ccagagcagc ctggtacttg gctgccggtc cgtccggcac cggaccctcg
     2221 gtgagggcgg cgaaggtcgc gatggggaag ggggccgccg gcgtgggcga ggtcgccgac
     2281 ggagagggcg ccgtcgtgga tccggcggag ggcgatccgt cgcttcgacc tggtcctgag
     2341 gaacacccgg tcagggccac cgccagtacc aggagggtga tccgcccaga ccggaccatc
     2401 acggtcacac acctttggct ccgatcgacc tcgcgggtat gcgtcgccat cagctggtgt
     2461 ggaaccggac ggagtcgatg actggatcag cttcctcgag ggcttcttcg aagctcccct
     2521 tgggtgccat gacggtgatc gccagggtcc gcatcgacat gcccttgggc aggtcgacga
     2581 ggaacaggcg caggcgcttc cctggttcca gagcgtggat ccagcgctga gcgtcggagc
     2641 ggtatgccgt gcagacgcgg ccggtggggg acaactcgac atcgatcgtt acggctttgg
     2701 caccgccgac gcgcgcggcg accggcgaag tggattcgaa gttgcggtcc gcgatgaccg
     2761 cctgcgcgaa cgcagcagca tcggccggca ccgcgcctcg gccacactcc gaccagaaga
     2821 cggcgggatc ggtgaagtcg aggagaccgc tcgggtcgcg ctcatctccg atgaccatcg
     2881 ggttctggct cggcgacgaa taggtgacct cgccgtcgaa gtgcgcgaag ggcatgggga
     2941 ccggccggcc gttctccgtc gtcctggctc cgagcgccag cccgccggtg gtccctgccg
     3001 acacgcctgc gtcccagtgc gagtggatct cctgctccac cacgcttcca tcctcggcga
     3061 agaggcggat cctgaactcg atgtagcctc cgtagggcca ggccggtccc gccacacgtt
     3121 cgaactccga tcgctcgtac gaggagcccg agctcgtggc gtagagcagc gggacctctg
     3181 gttcgatcca atcggcgtag acgtcgacgt acccctcggc ccctcgtcca gcgacccgtg
     3241 ccgccaggaa ctcctccaag cgcctggttg cctgcgcctc ggcgaccgcc gggtcggctt
     3301 gcgtgaacgt cgtcgtccgc cagttgctca cgacccagat cccaccccgc cccttgcgac
     3361 ccggctgcac gaggtcgagg ctcaccgact cgtaggtgag ctcgtcgagc gtcggcgcgc
     3421 agcgttcgcc ggggttccgc gaatcaggct gtggtgggta gagcgggttc gtgcggcccg
     3481 gcgcacaacg caggaaccgt tggtcggtga gtgagtggtc agcgccaccg acgtggaggt
     3541 tctgcatgta tgcctcccag cccaactcct cacggaggaa ccggtcgacg agttcgacct
     3601 caccgttgta gaggcggttc cacgtgccgt cgtgatcttc ggccgcgagc tgggcgtcga
     3661 gctgccacgt gtagtccgag tcaccggcgt cagcgcgctc ctgggcctgc ttgacctcct
     3721 cgaggttcgc ctgcggccac atgccgcccg acagtgtgtc cggcttgctg cccttccgct
     3781 cccatacc
//
LOCUS       DATLGG010000082         6532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_132419, whole genome shotgun sequence.
ACCESSION   DATLGG010000082 DATLGG010000000
VERSION     DATLGG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132419"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..432
                     /locus_tag="VM638_02270"
     CDS             229..432
                     /locus_tag="VM638_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11290.1"
                     /translation="MAYPATHLQEEQLLTRYLRGLVRTGGSAPDRSPGYQTRTCANCG
                     QHTKFVLDPSGVWYSCSSCQRLA"
     gene            503..775
                     /locus_tag="VM638_02275"
     CDS             503..775
                     /locus_tag="VM638_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005296245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HVM11291.1"
                     /translation="MYGYSMNKDQLLARLRRIEGQVRGLARMVDEDAYCIDILTQLTA
                     VRSALHGVGLGLLDDHVRHCVRESVASGEDDEKIQELVDAVARIVR"
     gene            785..994
                     /locus_tag="VM638_02280"
     CDS             785..994
                     /locus_tag="VM638_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HVM11292.1"
                     /translation="MADARIQVPEIHCAHCKHSIETAVSKVPGVEDVTVDIESRSVAV
                     RYDDAVTGTDPIHAAIEEQGYEVPR"
     gene            991..3123
                     /locus_tag="VM638_02285"
     CDS             991..3123
                     /locus_tag="VM638_02285"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVM11293.1"
                     /translation="MTRSDHEVVLDVEGMTCSSCAVRIERVLERQPGVQEASVNFANA
                     RATVTIDDGRAPIDEVLSAVERIGYRASPMEEGAAHDHGREARSWLRRVVVSWPLAMA
                     TMVLSMAFMHERWARLASFALATPVQLWVGWPFLRGAALRARRFVANMDTLIAIGTLA
                     AYGYSLWALATGGELYFETAAVILAFISLGRYLEARAKGSASRALARILDLGAKKAYV
                     LRDGQEVLVPVDQVQVGDRVRVRPGERVPVDGSVVEGTTSVDESMLTGEPVPVEKRPG
                     DDVSAGTINTTGSVVFEATRVGEATTLARVARLVEDAQAGKARLQRLADRVSGIFVPV
                     VLAIATATFAGWLVATGSVRDALIPAVAVLIIACPCALGLATPTAVMVGTGRAAQLGA
                     VVRGAEVLEHARTIDTVVLDKTGTLTEARMQIQSVVGDPDALRFAATVEAGSEHPLAR
                     AVVDGARARNLPLPAVSDFRSVEGRGVIGHVEGVTVVVGRRSLVEEMAEIPEDLDRDV
                     RGHQEAGGTASYVAWDGKARGALVVGDTVRPGAGPAVARLRELGIDVVMLTGDAEAAA
                     RTVATEVGIERVVAGVLPDGKIGEIRRLQAEGRRVAMVGDGVNDAPALAQADLGIAVG
                     SGTDVAIEAAGITLMRPDPRVIPATILLARRTYRTIVQNLWWAFGYNVAAIPLAAAGL
                     LSPVVAAAAMALSSVSVVANSLRLRRTR"
     gene            complement(3127..3528)
                     /locus_tag="VM638_02290"
     CDS             complement(3127..3528)
                     /locus_tag="VM638_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVM11294.1"
                     /translation="MRTDRFTVDTGGARVVDITERVGAFAAGGGDGLLNVFLMHATAG
                     LALIETGAGSERDLIEALERLLPRDDRYDHRHGSRGHGADHLLPAFVAPSLVLPVEDG
                     TPVLGTWQRVVVVDLNRENNRRTVVLSLVEG"
     gene            complement(3528..3869)
                     /locus_tag="VM638_02295"
     CDS             complement(3528..3869)
                     /locus_tag="VM638_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HVM11295.1"
                     /translation="MKEMVTFDDFERVDIRAGTVLRCEPFPEARKPAYKLWIDFGPEI
                     GTRRSSAQITTHYEPSALVGRQVLAVVNFPPRQVGPFMSEVLTLGVYAEDGSVVLVTP
                     DRRVQDGARFG"
     gene            complement(3889..4467)
                     /locus_tag="VM638_02300"
     CDS             complement(3889..4467)
                     /locus_tag="VM638_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVM11296.1"
                     /translation="MSERNELLAEIEREFSSRVVHQLKTPLALMHGYAATLLERDLSE
                     EQRRWFLDVIRRQAASLATMLDRLEDLRRLHEAEDGRGSLRAAVSTAAGELAEGGPLE
                     LRSRDVPETDVPVPARWLARALVAAATAFPEATDEPRSLEVSLEGEVLALVGSTAPRE
                     RSIGFYVAGRILERFGGSLEAEGARVSLRLPT"
     gene            4510..5169
                     /locus_tag="VM638_02305"
     CDS             4510..5169
                     /locus_tag="VM638_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM11297.1"
                     /translation="MKLLVLDDHAIIRDGIRLLAEQTEGIDLVAEAGTVADALTHVER
                     ERPDVALVDLDLPAGSGLNFIREAGKRFPGVRCLVLTVDRSEETVLEAMNAGASGFVT
                     KESSLPSILDAARRVAAGETVVEGLSVGRLVERFAGFAEETGRSAEILSRLSDREREV
                     LALLASGLTNQQIGSRLGITARTASTHVANIYRKIEVTNRVDAARAAMRLGLTPGPAE
                     G"
     gene            5245..6306
                     /locus_tag="VM638_02310"
     CDS             5245..6306
                     /locus_tag="VM638_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVM11298.1"
                     /translation="MRTIAIEEGAVTAPVIAGLEDLGFSLWYLPRTGTREIRRLIEEF
                     RPDVAVLPLEPRTMEAGEALPRVLPSVRLVASTAGNESEVRPPWADLTIPKDADPIFW
                     LLSLLALQPRTDAASPALAEAGAGWRRTLLVAIERTHHAFRAIDDRLQRRSDAMLVEL
                     RTRLEASFEQLLHLLMDRLEAEVPGMAGHSSRVADLAGRLAQVLGLDRRQTEVVRMSG
                     LLHDLGMHLVLPPVSLRRRGPLHQGEWDVLRAHPVASAAVLAPLPQGIQIGRAIREHH
                     ERLDGSGYPLSKRGEEVSIEARVVTVADAFEAMTHPRPHREAMSADPAIEALRAEARN
                     GRFDPEVCSALVSAAGARG"
BASE COUNT          988 a         2243 c         2338 g          963 t
ORIGIN      
        1 cccgagcgtc gagaacatca tcgtcgtcag cacggccacg ctcagcacgc gcgcgaaggt
       61 tcgggcgtgg gacatcctgg tctccttccg ttccaagggc gtagatcgga aaggcgggga
      121 atttccggca ccctatccgc acgggtgggg cgttgcaaac aacctcgacc catggccgtt
      181 tcgaccgacc ttcgcgcggg aatgctctcg ttgcgaaagg agagacccat ggcatacccg
      241 gcaacgcacc tccaggaaga gcagctcctc acccggtacc tgcgtggctt ggtccggacc
      301 ggaggctccg cgccggatcg ctcccccggg taccagaccc gcacgtgcgc gaactgcggt
      361 cagcacacga agttcgtgct cgacccgtcg ggcgtctggt acagctgctc ctcgtgccag
      421 cgcctcgcct gagctgacct gctccggctt atacccccca ggggtaccgt gctatcatgg
      481 acctccccgt cgaggaggaa cgatgtacgg ctactccatg aacaaggatc agctcctggc
      541 gcgcctgcgt cggatcgagg ggcaggttcg cgggctcgcc cggatggtcg atgaggatgc
      601 gtactgcatc gacatcctca cgcagctgac cgccgtccgg tcggccctcc acggtgtggg
      661 cctcggcctc ctcgacgatc acgtccgcca ctgcgtccgc gagagcgtcg cctccggtga
      721 ggacgacgag aagatccagg agctcgtgga cgccgtggcc cgcatcgttc gctgaggagg
      781 agccatggcc gacgcacgca tccaggtccc cgagatccac tgcgcccact gcaagcactc
      841 gatcgagacc gcggtctcga aggtccccgg tgtcgaggac gtcaccgtcg acatcgagag
      901 tcgaagcgtc gcggtccgct acgacgacgc ggtgaccggc acggacccga tccatgcggc
      961 gatcgaggaa caggggtacg aggtcccgcg gtgacgcgct ccgatcacga ggtcgtcctc
     1021 gacgtcgagg gtatgacgtg ctcctcgtgc gcggtgcgga tcgagcgcgt cctcgagcgt
     1081 cagcctggcg tccaagaggc ctccgtcaac ttcgccaacg cgcgggccac cgtcactatc
     1141 gacgacggga gggcgcccat cgatgaggtt ctctcggccg tcgagcgcat cgggtaccga
     1201 gcctcgccga tggaggaggg ggcggcccac gaccatggca gggaggcgcg ctcgtggctc
     1261 cgccgggtgg tggtctcgtg gcccctggcg atggcgacga tggtgctctc gatggcgttc
     1321 atgcacgagc gctgggcccg gctcgcgtcg ttcgcgctgg cgaccccggt gcagctctgg
     1381 gtcgggtggc cgttcctgcg tggggcggcc ctgcgtgcgc gccgcttcgt tgcgaacatg
     1441 gacaccctca tcgcgatcgg aacgctcgcg gcctacggct actcgctgtg ggcgctcgcg
     1501 accggcggag agctgtactt cgagaccgcc gcggtgatcc tcgccttcat ctcgctcggt
     1561 cgctacctcg aagcgcgtgc gaagggaagc gcatcgcgcg ccctcgctcg gatcctcgat
     1621 ctcggagcga agaaggcgta cgtcctccgc gacgggcagg aggtcctggt tccggtcgac
     1681 caggtccagg tcggcgaccg cgtccgcgtg cggccgggtg aacgcgttcc ggtcgatggg
     1741 tcggtcgtcg aaggaacgac gagcgtcgat gagtcgatgc tgaccggtga gccggttccg
     1801 gtcgagaagc ggccgggcga cgacgtttcg gccggaacga tcaacaccac gggaagcgtc
     1861 gtgttcgagg cgacgcgcgt cggggaggcg acgacgctgg cacgggtcgc gcgtctcgtc
     1921 gaggatgccc aggcggggaa ggcgcgcctc cagcgtctcg cggatcgggt gtcggggatc
     1981 ttcgtccccg tcgtgctcgc gatcgccacg gcgacgttcg ccggctggct cgtggcgacg
     2041 ggaagcgtgc gcgacgccct gatccctgcg gtggccgtgc tcatcatcgc gtgcccgtgc
     2101 gcgctggggc tcgcgacccc aacggcggtg atggtcggaa ccggcagggc ggcgcagctc
     2161 ggtgccgtgg tgcgtggtgc cgaggtcctc gagcacgcac gaacgatcga caccgtcgtc
     2221 ctcgacaaga ccggaacgct caccgaggcg cggatgcaga tccagagcgt cgtcggcgac
     2281 cccgacgccc tgcggttcgc cgccacggtc gaggcgggct ccgaacatcc gctggcgagg
     2341 gccgtcgtgg acggggcgcg cgcgcgcaac ctcccgctgc cggcggtgag cgacttccgc
     2401 tcggtggaag gccggggcgt gatcggacac gtcgaaggcg taacggtcgt ggtcggccgg
     2461 cgctccctcg tggaggagat ggcggagatc ccggaggacc tcgaccgcga cgtccgtgga
     2521 caccaggagg ccggaggcac ggcctcctac gtggcctggg acgggaaggc gaggggggcc
     2581 ctcgtcgtcg gcgacaccgt gcgtccaggc gccggcccgg cggtggcgcg tctccgcgag
     2641 ctcggcatcg atgtcgtcat gctcaccggg gacgccgaag ccgcggcgcg cacggtcgcc
     2701 accgaggtcg ggatcgagcg ggtggtggcg ggggtgctgc ccgatgggaa gatcggggag
     2761 atccggcggc tgcaggccga aggacggcgc gtggcgatgg tgggcgacgg ggtgaacgac
     2821 gcgccggcgc tcgcgcaggc ggacctcggg atcgcggtcg gttccgggac cgacgtcgcg
     2881 atcgaagccg ccggcatcac gctgatgcgc cccgacccaa gggtgatccc ggcgacgatc
     2941 ctcctcgcac gtcgcaccta ccgaaccatc gtgcagaacc tctggtgggc cttcggatac
     3001 aacgtggcgg cgatcccgct ggcggccgcc ggactcctgt cgccggtcgt cgccgcggcc
     3061 gcgatggcgc tgtcctccgt gtccgtcgtg gcgaactcgc tgcggctgcg gcgcacgcgc
     3121 tgagggtcag ccctcgacga gggagaggac gaccgtgcgt cggttgttct cccggttcag
     3181 gtccacgacc acgacacgct gccacgtccc gaggaccggc gttccgtcct ccaccggcag
     3241 gacgagcgag ggggcgacga aggcgggaag gaggtgatcc gccccgtggc cgcgcgagcc
     3301 gtggcggtgg tcgtagcggt cgtcgcgggg aagcaggcgc tccaacgcct cgatgaggtc
     3361 gcgttcggag ccggcgcccg tttcgatgag ggccagcccc gcggtggcgt gcatgaggaa
     3421 gacgttgagc agaccgtctc ccccaccggc cgcgaacgca ccgacacgct cggtgatgtc
     3481 gaccacgcgg gcgcccccgg tgtcgacggt gaatcgatcc gtgcgcatca gccgaaccgc
     3541 gcgccgtcct ggacgcgacg gtccggcgtc acgaggacga cgctcccgtc ctcggcgtag
     3601 acacccagcg tcagcacctc ggacatgaac ggccccacct gccgcggcgg gaagttcacc
     3661 accgcgagga cctggcgccc gacgagcgcc gacggctcgt aatgggtcgt gatctgcgcg
     3721 ctcgaacgcc gcgttccgat ctcggggccg aagtcgatcc agagcttgta ggcgggcttc
     3781 ctcgcctcgg ggaagggttc gcacctgagc accgtgccgg cccggatatc cacgcgctcg
     3841 aaatcgtcga acgtcaccat ctccttcacg gcggccacga tacccgtctc aggtgggcaa
     3901 ccggagggag acccgagctc cctccgcctc gaggctgcca ccgaagcgtt cgaggatccg
     3961 gcccgccacg tagaagccga tcgatcgctc gcgcggcgcc gtcgaaccga cgagcgcgag
     4021 gacctcgccc tcgagcgaga cctcgaggct gcggggctcg tcggtggcct cggggaaggc
     4081 cgtcgcggcc gccacgagcg cccgcgcgag ccaccgagcc gggaccggga catccgtctc
     4141 ggggacgtcg cggctgcgga gctcgagcgg tcccccctcg gccagctccc cggccgccgt
     4201 cgagaccgct gcgcgcaggg acccgcggcc atcctcggcc tcgtgcagac gccggaggtc
     4261 ttcgaggcgg tccagcatcg tcgcgaggga ggcggcctgc cggcggatca cgtcgaggaa
     4321 ccatcgccgc tgctcctccg agagatcgcg ttccaggagc gtggcggcgt acccatgcat
     4381 cagggcgagc ggcgtcttga gctgatggac gacccgggag gagaactcgc gctcgatctc
     4441 cgcgagaagc tcgttccgct cggacacatg cctatagtag aaggaccgat cgatcaggga
     4501 tctctcgccg tgaagctgct cgtgctcgat gaccacgcga tcatccgcga cggcatccgc
     4561 ctcctcgccg agcagaccga gggcatcgac ctcgtcgccg aggccggcac cgtcgctgac
     4621 gcgctgacgc acgtcgagcg tgagcggccc gacgtcgcgc tcgtcgacct cgaccttccc
     4681 gcgggctccg gcctgaactt catccgcgag gcggggaagc ggttcccggg ggtccgctgc
     4741 ctcgttctga cggtcgatcg cagcgaggag accgtcctcg aggcgatgaa cgccggggca
     4801 tcggggttcg tcacgaagga gagcagcctc ccctcgatcc tcgacgcggc ccggcgggtc
     4861 gcagcgggcg agacggtcgt cgaaggcctg tcggtggggc gcctggtcga gcgcttcgcc
     4921 ggcttcgccg aggaaacggg gcgctcggca gagatcctgt cgcgcctgtc ggatcgggag
     4981 cgcgaggtgc tggcgctgct ggcgagcggc ctcaccaacc agcagatcgg gtcgcggctc
     5041 ggcatcaccg cgcggacggc gagcacgcac gtcgccaaca tctaccggaa gatcgaggtc
     5101 acgaaccggg tcgacgcggc gcgggccgcc atgcggctcg ggctcacccc cggtccggct
     5161 gagggctgag atacgccgaa ctacgtatca agcctcgtac gttcgcccga tgccggcctc
     5221 ggtcggggcg cgtacgctgg acgcatgagg acgatcgcga tcgaagaggg tgccgtgacc
     5281 gcaccggtca tcgccggcct cgaggacctc gggttctcgc tctggtacct gcccaggacc
     5341 ggcacgaggg agatccgccg cctgatcgag gagttccgac ccgacgtggc cgtcctcccc
     5401 ctggaacccc gcacgatgga ggccggggaa gcccttccac gcgttcttcc gagcgtccgc
     5461 ctcgtcgcca gcaccgccgg caacgaatcg gaagtgcggc ccccgtgggc cgacctcacg
     5521 atcccgaagg atgccgaccc gatcttctgg ctcctgtccc tgctggccct ccagccccgc
     5581 accgacgccg cttctccggc cctcgcggaa gccggggccg gatggcgccg aaccctcttg
     5641 gtcgcgatcg agcgaaccca tcacgcgttc cgggcgatcg acgaccggct gcagcggcgg
     5701 tccgacgcga tgctcgtcga gcttcgcacc cgcctggagg cgtcgttcga gcagctcctc
     5761 cacctcctga tggaccggct ggaggccgag gtgcctggca tggccggcca ctcgagccgc
     5821 gtggccgatc tcgccggtcg cctcgcgcag gtcttggggc tcgaccgccg tcagacggag
     5881 gtggttcgga tgtcggggct cctgcacgac ctcgggatgc acctcgtgct gccaccggtc
     5941 tcattgcgtc ggagagggcc cctccaccag ggagaatggg acgtgctgcg cgcgcatccc
     6001 gtggcctcgg ccgccgtgct cgcccccctt ccccaaggga tccagatcgg gagggcgatc
     6061 cgagagcacc acgagaggct ggacggcagc gggtaccccc tcagcaagcg aggcgaggag
     6121 gtttcgatcg aggcgcgggt ggtaacggta gctgacgcct tcgaagcgat gacgcaccct
     6181 cgaccacacc gtgaggcgat gtcggccgat ccggcgatcg aggcgctccg cgcggaggca
     6241 cggaacgggc gattcgaccc cgaagtctgc tctgcgctag tctccgcggc gggagcacgc
     6301 gggtagatgg ctcccctttt tgccgtagga caaaatgcgc acttggcagg agggcgggtg
     6361 accgacgtcg aatacgtagt agtacggacg cgcgggagcg cagaaggagc ggggcaccga
     6421 tgacctacaa cggcaaacat cgtccagagc ggaggagtcg taggagcatg gtcgcgatgg
     6481 cggccctcct cgtcctttcc atggttgcgg tccagggatc gctggcgacg gc
//
LOCUS       DATLGG010000083         6507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_52727, whole genome shotgun sequence.
ACCESSION   DATLGG010000083 DATLGG010000000
VERSION     DATLGG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52727"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1260
                     /locus_tag="VM638_02315"
     CDS             22..1260
                     /locus_tag="VM638_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HVM11299.1"
                     /translation="MSATEADVLVVGGGPAGAATAAHLARNGADVVVVDKATFPREKV
                     CGDGLTPRGVKALHRVGVDPTEPGFVSVRGLRTHGAGVKLELPWPRLRDFPDFGVVRT
                     RHDLDHLLLQRAQKDGARVWEGAEALEPRVERGWVAGAMVRRQPDAAPVEVRARFVVA
                     CDGASSRFAQRAGVRRDPSRPIGVAARRYYRSPHDPGGMFESWLELWDGPRQLPGYGW
                     VFPVEPGLLNVGAGLINTYRRFRDVSARHAFDVFARGLPPEWALTEENAVGPVLSGPL
                     PTSLNRRPLATPGLLLVGDAAGAVNPFNGEGIAYALETGELAAELIHAAIERDRPALA
                     HLYPIELRRRYGRYFTLGRAFVKLIGNPAVMRAATRYGLPRRRLMAFLLRTMANLTDG
                     RDGDLEDRVLHALVRLAPER"
     gene            1307..1666
                     /locus_tag="VM638_02320"
     CDS             1307..1666
                     /locus_tag="VM638_02320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVM11300.1"
                     /translation="MALSEYLPIVLLAGLAFLFAIASLAASALLRPKRPIPAKLAPYE
                     CGIVPEHEPVERFPVRFYIVAMMFIIFDIETIFLFPWAVTFRQLGLFGLIEMVIFIVL
                     VFVAYVYVWQRGGLEWE"
     gene            1663..2136
                     /locus_tag="VM638_02325"
                     /pseudo
     CDS             1663..2136
                     /locus_tag="VM638_02325"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            2157..2654
                     /locus_tag="VM638_02330"
     CDS             2157..2654
                     /locus_tag="VM638_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVM11301.1"
                     /translation="MSPEDLLERVRARHADALLARGEITLTVPREEILETLRWLRDDE
                     ALGFRFFADVSCTDWPDREPRMWLAYHLARPDASARIRVKVGLPGDDLSVPSVTPEFP
                     AADWHEREMFDFFGVRFEGHPDLRRIMMPDGWEGHPLRKDHAMGGVSTPYHGASIPPA
                     DERGL"
     gene            2654..3925
                     /locus_tag="VM638_02335"
     CDS             2654..3925
                     /locus_tag="VM638_02335"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005476345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVM11302.1"
                     /translation="MQVPEELGGAPMAQGTTAERLREDLMVVNMGPQHPSTHGVLRLL
                     ITLDGETVVDLRPIIGYLHTGIEKNTEYRTWQQGVTLVTRMDYLSPFFNEFAYCRSVE
                     RLLGIEAPPRAEAIRVLLCELTRVASHLVWLATSGFELGAISMMLYGFRERERILDIF
                     EMISGLRMNHSFIRIGGLVMDLPDGAEEKIRAFLAEMPQRIDEYEALLSNNPIWLERN
                     KGVGILDAETCLRLATTGPLLRAAGVRHDLRLADPAWQPYDFEVPVRTEADCYARYEI
                     RIEEMRQSLRICEQVLDSLPGGPVMIQDPKIAWPAHLEVGPDGIGNSEAYVRHIMEES
                     MEALINHFKIVTQGIRVPPGEVYTAIESPRGELGYYVVSDGGVRPYRVRVRDPSFCNL
                     MSLPEMVRGSLIADVIACVASVDPVMGGVDR"
     gene            3925..4488
                     /gene="nuoE"
                     /locus_tag="VM638_02340"
     CDS             3925..4488
                     /gene="nuoE"
                     /locus_tag="VM638_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HVM11303.1"
                     /translation="MPVFDDDLRRRADELVARYPSSRSALLPLLYLAQSVEGYLSREG
                     MREVGDILGLSTAEVEAVATYYTMYKHEPTGRWLLSICTNVSCAVLGGRRLYERAKEL
                     LGEDAEKVTEDGAITLEEVECLGACDAAPVLQVNYCNYDRVSEEQLTEMIERLRSDDP
                     PEPARGPKPVDHARTSWILATMGDDRA"
     gene            4481..5827
                     /gene="nuoF"
                     /locus_tag="VM638_02345"
     CDS             4481..5827
                     /gene="nuoF"
                     /locus_tag="VM638_02345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HVM11304.1"
                     /translation="MPDFPRVITARWGIDGADTIDGYLEHDGYEGLRKALAMSPDEVI
                     DVVKSSGLRGRGGAGFPTGAKWGFIPRDTGKPVYVCCNADEGEPGTFKDRELMERDPH
                     QLIEGIAITSYAVGCRTAFIYMRGEFLEPARRMERAIAEAYERGFLGEGIAGSDFSLD
                     IVLHRGAGSYECGEETAQLESLEGKRGQPRLRPPFPAVEGLYRSPTVVNNVETLSTVP
                     HILHRGADWFRGIGTEHSSGTKIFCVSGKVARPGNYEAPMGTPARVIIDELAGGVPNG
                     KALKAWTPGGSSTPFLTADHLDTPMDFESIQEAGSLLGTGAMVVLDEDDCVVEAVLRW
                     EQFYAHESCGKCTPCREGTWWLVKILHRMENGGGRLEDLPIMQDVGSNILFKSLCALG
                     DGAVSPIHSSMKFFREEYERHVEANGCPYRERPVIAAATPRSGGAAGSIPVGEVIP"
     gene            5824..>6507
                     /locus_tag="VM638_02350"
     CDS             5824..>6507
                     /locus_tag="VM638_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVM11305.1"
                     /translation="MTDERVNVVVDGKEISVPKGTLIIRAAEALGVEIPRFCDHPMLE
                     PVGACRTCLVEVEGQRKLVTSCTTPVADGMVVRTQNTSEEASKGQQGQLEFLLINHPL
                     DCPVCDRGGECPLQDQTLAFGPAESRYIDPKRTYKKPIPLSPLVLLDRERCVLCARCT
                     RFCDQISGERFIELFERGAAEQVSISPGEDFESPFSGNTVQICPVGALTSSTFRFAAR
                     PFDLKMADSV"
BASE COUNT          939 a         2278 c         2294 g          996 t
ORIGIN      
        1 cttcaggggg ggctcgctga gatgtctgcg accgaggcgg acgtcctggt ggtcggcggg
       61 gggcccgccg gcgcggcgac cgcggcgcat ctggcccgca acggggccga cgtcgtcgtc
      121 gtcgacaagg ccaccttccc gcgcgagaag gtctgcggcg acggactgac gccccggggc
      181 gtgaaggcgc tccatcgggt gggcgtcgac ccgaccgagc ccgggttcgt ctcggtccgc
      241 ggcctccgga cccacggcgc cggggtgaag ctcgagcttc cgtggccgcg cctgcgcgac
      301 ttccccgact tcggtgtcgt gcggacccgc cacgacctcg atcacctcct cctccagcgc
      361 gcgcagaagg acggcgcccg ggtctgggag ggagccgaag ccctcgagcc gagggtcgag
      421 cggggctggg tcgcgggcgc gatggtccgg cggcagcccg acgcggcccc cgtggaggtc
      481 cgcgcccggt tcgtcgtcgc ctgcgacggc gcctcgtctc gcttcgccca gcgcgccggt
      541 gtccggcgag acccgagccg gccgatcggc gtggcggccc gccgctacta ccggagcccg
      601 cacgacccgg gcgggatgtt cgaatcgtgg ctcgagctgt gggacggccc acgtcagctc
      661 cccgggtacg gctgggtctt cccggtcgag ccgggcctgc tgaacgtcgg cgccgggctc
      721 atcaacacct accggcgctt ccgcgacgtc tcggcgcgcc acgcgttcga cgtcttcgcg
      781 cgtggcctcc cgcccgagtg ggccctgacc gaggagaacg cggtcggccc ggtgctgtcg
      841 gggccgctgc cgacctccct gaaccggcgg ccgctggcca ccccgggact cctgctcgtg
      901 ggcgatgcgg ccggagcggt gaacccgttc aacggcgagg ggatcgccta cgcgctcgag
      961 acgggggagc tggccgccga gctgatccac gcggcgatcg agcgggaccg gccggcgctc
     1021 gcccacctct acccgatcga gctgcgccgc cgctacggcc ggtacttcac gctcggtcgg
     1081 gcgttcgtga agctgatcgg gaacccagcc gtcatgcggg cggccacgcg gtacggcctc
     1141 ccgcgccgtc ggctcatggc gttcctcctt cggacgatgg cgaacctgac ggacgggcgg
     1201 gacggggacc tcgaagaccg cgtcctgcac gccctcgtgc gactggcgcc ggagcgatga
     1261 cggcccgcgt ttgcgccccg ttcgaccgcg tcgataggct cggccgatgg cgctgagcga
     1321 gtacctgccg atcgtcctgc tcgcggggct ggccttcctg ttcgcgatcg cctcgctcgc
     1381 cgcctccgcg ctgctgcggc cgaagcgtcc gatcccggcg aagctcgccc cctacgagtg
     1441 cgggatcgtc cccgagcacg agccggtcga gcgcttcccc gtccgcttct acatcgtcgc
     1501 gatgatgttc atcatcttcg acatcgagac gatcttcctc ttcccgtggg cggtgacgtt
     1561 ccgccagctc gggctgttcg ggctgatcga gatggtgatc ttcatcgtgc tcgtgttcgt
     1621 cgcctacgtc tacgtctggc agcggggtgg tctcgaatgg gaatgagccg cgaggagatc
     1681 gagcagcgca cggtcgagga ggaggcgaag cagggcatcc tcacgacgac cctcgggcgg
     1741 gcggtgaact gggcgcggaa gcagtcgctg tggcccgcca ccttcgggct cgcctgctgc
     1801 gcgatcgaga tgatgcacac gggcgcgggc catcacgacc tcgcgcggtg ggggatggag
     1861 gtcttccgcg cctctccgcg gcaggcggac ctcatgatcg tcgcggggcg cgtctcgcag
     1921 aagatggggc cggtcctgcg gcagatctac gaccagatgc cggagccgaa gtgggtcatc
     1981 tcgatgggcg tgtgcgcctc gcagggcggc atgttcaaca actacgccgt gatccagggc
     2041 gtcgacacga tcgtgccggt ggacatctac gtcccgggat gcccgccgcg gccggagatg
     2101 ctgatgtacg ggatcctccg cctgcacgac aagatctcga agcggggaac gttcaagtga
     2161 gcccagagga cctcctcgag cgcgtgcgcg cccggcacgc ggacgcgctg ctcgcgcggg
     2221 gcgagatcac gctgacggtg ccgcgcgagg agatcctcga gaccctccgc tggctccgcg
     2281 atgatgaggc gctcggcttc cgcttcttcg ccgacgtctc ctgcacggac tggccggacc
     2341 gcgagccgag gatgtggctc gcgtaccacc tcgcgcgacc ggacgcttcg gcccggatcc
     2401 gggtcaaggt cggccttccc ggcgacgacc tcagcgtccc atccgtgacg cctgagttcc
     2461 ccgcggccga ctggcacgag cgggagatgt tcgacttctt cggggtgcgc ttcgagggtc
     2521 acccggacct tcgccggatc atgatgccgg acggctggga ggggcatccc ctgcggaagg
     2581 accacgcgat gggaggcgtc tcgacgccct accacggcgc ctccatccct ccggccgacg
     2641 agcgaggtct ctgatgcagg tccccgagga gctgggcggc gcgccgatgg cgcaggggac
     2701 gacggccgag cgcctgcgcg aggacctgat ggtcgtgaac atgggtccgc agcacccgtc
     2761 cacccacggc gtgctccgtc tgctgatcac gctcgacggc gagacggtcg tcgatctccg
     2821 cccgatcatc gggtacctcc acacggggat cgagaagaac accgagtacc ggacctggca
     2881 gcagggcgtc acgctcgtga cgcgcatgga ctacctctcc ccgttcttca acgagttcgc
     2941 ctactgccga tcggtcgagc ggctcctcgg catcgaggct cccccgaggg cagaggcgat
     3001 ccgcgtgctg ctgtgcgagc tcacgcgggt ggcgagccac ctcgtctggc tggcgacgag
     3061 cgggttcgag ctcggcgcga tctcgatgat gctgtacggc ttccgcgagc gggagcggat
     3121 cctcgacatc ttcgagatga tctcgggcct gcggatgaac cactcgttca tccgcatcgg
     3181 gggcctcgtc atggacctgc ccgacggcgc cgaggagaag atccgcgcct tcctcgccga
     3241 gatgccgcag cggatcgacg agtacgaggc gctgctctcg aacaacccga tctggctgga
     3301 gcggaacaag ggcgtcggga tcctcgacgc ggagacctgc ctccgcctcg cgacgacggg
     3361 gccgctcctg cgcgccgccg gcgttcgtca cgacctccgg ctcgccgacc ccgcgtggca
     3421 gccctacgac ttcgaggtgc cggtccgcac ggaggcggac tgctacgcgc gctacgagat
     3481 ccgcatcgag gagatgcgcc agtcgctgcg gatctgcgag caggtcctcg actccctgcc
     3541 gggcggaccc gtgatgatcc aggacccgaa gatcgcgtgg cccgcccatc tggaggtcgg
     3601 ccccgacggg atcggcaact cggaggccta cgtgcgccac atcatggagg agtccatgga
     3661 ggcgctgatc aaccacttca agatcgtcac gcaggggatc cgggttccgc cgggcgaggt
     3721 ctacaccgcg atcgagtcgc cgcgcgggga gctcggctac tacgtcgtct ccgacggcgg
     3781 cgtccgtccc tatcgcgtgc gcgtgcgtga cccctcgttc tgcaacctga tgtcgctgcc
     3841 ggagatggtc cggggttcgc tcatcgccga cgtgatcgcc tgcgtcgcga gcgtcgaccc
     3901 ggtgatgggt ggggtggacc gctgatgccg gtgttcgacg acgacctccg acgccgcgcc
     3961 gacgagctcg tcgcgcgcta cccgagctcg cgctccgcgc tcctgcccct gctgtacctg
     4021 gctcagtcgg tcgagggcta cctgtcccgc gaggggatgc gcgaggtcgg ggacatcctc
     4081 ggcctgagca ccgccgaggt ggaggcggtg gcgacctact acacgatgta caagcacgag
     4141 ccgaccgggc gctggctcct ttcgatctgc acgaacgtct cgtgcgccgt cctcggcggg
     4201 cggcggctct acgagcgggc gaaggagctc ctcggcgagg acgccgagaa ggtgaccgag
     4261 gacggcgcga tcaccctcga ggaggtcgag tgtctcggcg cttgcgacgc ggcgccggtg
     4321 ctccaggtga actactgcaa ctacgaccgt gtcagcgagg aacagctcac cgagatgatc
     4381 gagcgcctcc gcagcgacga cccacccgag cccgcccgcg gtccgaagcc cgtcgaccac
     4441 gcgcgcacct cctggatcct cgcgacgatg ggggacgacc gtgcctgact tcccgcgcgt
     4501 gatcacggct cgctggggca tcgacggcgc cgacacgatc gatggctacc tcgagcacga
     4561 cggctacgag ggtcttcgga aggccctcgc gatgtcgccg gacgaagtga tcgacgtcgt
     4621 gaagtcgagc ggtctccgcg gccggggcgg cgccggcttc ccgaccggcg cgaagtgggg
     4681 gttcatcccg cgcgacaccg gtaagcccgt ctacgtctgc tgcaacgccg acgaaggcga
     4741 gccggggacc ttcaaggatc gcgagctgat ggagcgcgac ccccaccagc tgatcgaggg
     4801 gatcgcgatc acctcgtacg cggtcggatg ccggacggcg ttcatctaca tgcgcggcga
     4861 gttcctcgag cccgcgcggc ggatggagcg ggcgatcgcc gaggcgtacg agcgcggctt
     4921 cctcggcgaa ggcatcgccg ggagcgactt ctcgctcgac atcgtcctgc accgcggggc
     4981 cggctcgtac gagtgcggcg aggagacggc ccagctcgag tccctcgaag ggaagcgcgg
     5041 ccagccccgc cttcggccgc cgttccccgc cgtcgagggc ctctaccgga gcccgaccgt
     5101 cgtgaacaac gtcgagacgc tgtccaccgt gccgcacatc ctgcatcgcg gcgcggactg
     5161 gttccgcggg atcggcaccg agcattcctc ggggacgaag atcttctgcg tctccgggaa
     5221 ggtcgcgcgc ccgggcaact acgaggcgcc gatgggaacg ccggcgcgcg tgatcatcga
     5281 cgagctcgcc ggtggcgtgc cgaacggcaa ggcgctcaag gcgtggacgc cgggcggctc
     5341 ctccaccccc ttcctcaccg cggaccacct cgacacgccc atggacttcg agtcgatcca
     5401 ggaggccggc tcgctcctcg ggaccggtgc gatggtcgtc ctcgacgagg acgactgcgt
     5461 cgtcgaggcg gtcctgcgct gggagcagtt ctacgcgcac gagtcgtgcg gcaagtgcac
     5521 gccgtgccgc gaagggacat ggtggctcgt gaagatcctg caccggatgg agaacggcgg
     5581 cggacgtctc gaggacctcc cgatcatgca ggacgtcggc tcgaacatcc tgttcaagtc
     5641 gctctgcgcg ctcggtgacg gcgcggtgtc gccgatccac tcgtcgatga agttcttccg
     5701 cgaggagtac gagcgccacg tcgaggcgaa cggctgcccg tacagggagc gcccggtcat
     5761 cgcggccgcg acgccgcgct ccggcggcgc cgccggatcg atcccggtcg gggaggtgat
     5821 cccgtgacgg acgagcgcgt gaacgtcgtc gtcgacggca aggagatcag cgttccgaag
     5881 ggcacgctca tcatccgtgc cgcggaagcg ctcggcgtcg agatcccgcg gttctgcgac
     5941 cacccgatgc tcgagccggt cggggcctgc cggacgtgcc tcgtcgaggt cgagggtcag
     6001 cgcaagctcg tcacctcgtg caccacgccg gtcgccgacg ggatggtcgt gcgaacgcag
     6061 aacacgagcg aggaggcgtc gaagggccag cagggccagc tcgagttcct gctgatcaac
     6121 catcccctcg actgcccggt ctgcgaccgc ggcggcgagt gcccgctcca ggaccagacg
     6181 ctcgcgttcg gccccgccga gtcccggtac atcgacccga agcgcacgta caagaagccg
     6241 atccccctgt cgccgctcgt gctcctcgac cgcgagcgct gcgtgctgtg cgcgcgctgc
     6301 acgcggttct gcgatcagat ctcgggcgag cgcttcatcg agctgttcga gcgcggcgcg
     6361 gcggagcagg tctcgatctc gccgggtgag gacttcgagt cgccgttctc cgggaacacc
     6421 gtccagatct gcccggtcgg ggcgctcacc tcgagcacgt tccggttcgc cgcccggccc
     6481 ttcgacctca agatggccga ctccgtc
//
LOCUS       DATLGG010000084         4681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_52852, whole genome shotgun sequence.
ACCESSION   DATLGG010000084 DATLGG010000000
VERSION     DATLGG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52852"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VM638_02355"
     CDS             <1..463
                     /locus_tag="VM638_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11306.1"
                     /translation="GDGYIRSRGGLDAARESLRWAEEGARSAGRDPAGLGFAVLQNAF
                     VWEDGEAWPVVRDGVAHQLGVYPAWYAGADTPGRGFEIHPPPDEKLRELTPAGDRHDV
                     LRALRPIVDTFGDRREFHLVVRLHYPGMRLETAARAVELFAGDVLPALTGS"
     gene            502..861
                     /locus_tag="VM638_02360"
     CDS             502..861
                     /locus_tag="VM638_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVM11307.1"
                     /translation="MPTVEDLMTRQPVTVTPSTTVAEAATMMGERKVGSALVMEAERL
                     VGIFTERDVLRALGEHFDAAGHPVSEWMTADPVTVPPTTEKAVALKTMLDGNFRHLPV
                     LEGGQVVGVVSMRDLSA"
     gene            complement(865..1059)
                     /locus_tag="VM638_02365"
     CDS             complement(865..1059)
                     /locus_tag="VM638_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11308.1"
                     /translation="MAREIDWQLFEKAVDLTAMALRGGMGGEGSQPPAFAAQVFTEVW
                     GALKQAAGDLPEKPSAGFSA"
     gene            complement(1157..1996)
                     /locus_tag="VM638_02370"
     CDS             complement(1157..1996)
                     /locus_tag="VM638_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11309.1"
                     /translation="MKVHRIATPLVLVAALALAPGATAHPGPATHVDSLSATVSGSFL
                     NVSGTATFVDVPFTAADDPSGDATPAGIGADITKATISRAPNGSTLVFRLDVADMPAL
                     GSTGIRYGWGLSVNGNDTGLYLGASRIGSCGTNGCFTLSRSSGSTTSVVANLTGSITA
                     TSVTWNVPMATLGVTRGSTVGAGAALTGSITGGEGLGHCCFIIDGFTAGDYRVPGAIV
                     ELGLAPAGAQPSTANFLEVATVDAATGAFSGSLPKPQSGSWQVVAKACYVATVCGYGS
                     LTL"
     gene            2225..3925
                     /locus_tag="VM638_02375"
     CDS             2225..3925
                     /locus_tag="VM638_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11310.1"
                     /translation="MKRTLVLAGALALGLLSGLTPASAVSTPVATQENVRHLATVPGN
                     TGGHVVAEGNRLYMGNYGTGLSAYDISDPRNPVKIGQYLPGPSATDGVDTGVRADAVP
                     DAAIWDGRHIVSLGGTLRGPNRVQTEFIDFTDPANPQLLRRFAGSNLSGGADGESHNG
                     DIVDARKLFLPSGGTGNNGLRIYDMRPLLNSPAGAPARLGGWNPHSLWQNSPYRINYG
                     KAPGSVFDHTHDLEVYTDLRVLLPEWEWEDQDEDGEPDPTYGNKDVVLLAAALGGNNG
                     GAVYVIDITNPRAPVAMGKWENTSGNTIDYLHEVQFLHGDPSTLVVTDEHMTAGCAEG
                     ALYTVRISENLSHMTKLGEWTIDGGAHDTPGTCMGSHVISSHDRHVFMGAYTAGLQVI
                     DLRNPAQPRRAGRYIAEGQNSWGALYHQGVVYTGDFGGRGLDVFEFIKDPVAKAFVKA
                     GHPQTAGNGTFDPIYGVSEELAKCAPDSQLNGVDGFWVRIPEDKRDGTATFRALGSGP
                     AGLYDVDVWFMNANCSQYVEPHLASEGTDEEGLIPEGAAYAVVSLYMGSPQWVYAQIL
                     "
     gene            4062..>4681
                     /locus_tag="VM638_02380"
     CDS             4062..>4681
                     /locus_tag="VM638_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11311.1"
                     /translation="MRRTLLLAAATSLALLAGLTPAGAQGLSTPLATQENVRHLASVP
                     GNTGGHVVVEGNRLYMGNYGTGLSVYDISNVRAPVKIGQYLPGPSTNDGVDPGARADA
                     VPDAAVWDNRHIVSLNGTSRASTTTQTEFIDFTDPAKPVLLHRFRGSSDGEAHNGDFV
                     DARRLWLPSGGSGNNGLRIYDVNPVLESPAKAPTRLGGWNPHTLWQ"
BASE COUNT          744 a         1671 c         1610 g          656 t
ORIGIN      
        1 gggtgacggc tacatccgca gccggggcgg gctcgacgcg gctcgggagt ccctccgttg
       61 ggccgaggag ggcgcgcgga gcgccgggcg cgaccccgcc gggctcgggt tcgcggtcct
      121 tcagaacgcc ttcgtctggg aggacgggga ggcgtggccg gtcgtgcgcg atggggtggc
      181 gcaccagctc ggggtctacc ccgcctggta cgccggcgcc gacacccccg gtcgcgggtt
      241 cgagatccat cccccgcccg acgagaagct ccgcgagctg acgcccgccg gggaccgcca
      301 cgacgtcctg cgcgcgctcc gcccgatcgt ggatacgttc ggcgatcgcc gtgagttcca
      361 cctcgtcgtg cgcctgcact atccgggcat gcgcctcgag acggccgctc gggcggtcga
      421 gctgttcgcg ggcgacgtcc ttcccgccct caccggctcc tgacctggcc ccgcggcccc
      481 gccgcgtcgt agagtgaccc catgccgacg gtcgaggacc tcatgacgcg tcagccggtg
      541 accgtgacgc catcgaccac cgtagccgag gcggccacca tgatgggcga gcgcaaggtc
      601 ggctccgcgc tcgtgatgga ggccgagcgt ctggtcggca tcttcaccga gcgcgacgtc
      661 ctgcgcgcgc tcggtgagca tttcgacgcg gccggtcacc ccgtatccga gtggatgacc
      721 gcggaccccg tgacggtccc gccgacgacc gagaaggccg tcgccctcaa gacgatgctc
      781 gacgggaact tccgtcacct cccggtcctg gaaggcgggc aggtcgtcgg cgtcgtgtcg
      841 atgcgcgacc tgtcggcctg agccctacgc cgagaaaccg gcgctcggct tctccggcag
      901 atcacccgct gcctgcttga gggcgcccca gacctcggtg aagacctggg cggcgaacgc
      961 cggcggctgc gacccctcgc cccccatccc accgcggagc gccatcgcgg tcaggtccac
     1021 ggccttctcg aagagctgcc aatcgatctc tctcgccatc tggatgcctc ccgtgtgtcg
     1081 ttcacgcgag cctatccaag atgcggacga gggacggccg gaggccgccc ctcgttgagt
     1141 cccgcgacgc ctgccgtcag agcgtcagcg agccgtaccc gcagaccgtc gcgacgtagc
     1201 aggccttcgc gacgacctgc cacgaaccgg actgcggctt cggcaggctt ccggagaagg
     1261 cgcccgtggc cgcatccacc gtcgccacct cgaggaagtt tgcggtgctg ggctgggctc
     1321 cggccggggc gagccccagc tcgacgatgg cccccgggac ccggtagtcc ccggcggtga
     1381 agccgtcgat gatgaagcag cagtgcccga ggccttcgcc accggtgatc gagccggtga
     1441 gggcggcgcc ggcgccgacc gttgagcccc gggtgacacc cagggtcgcc atcgggacgt
     1501 tccacgtgac cgaggtcgcg gtgatcgatc cggtcaggtt ggcgacgacg gaggtcgtgc
     1561 tcccggagga gcgcgaaagg gtgaagcagc cgttcgtgcc gcaggagccg atccggctcg
     1621 cgccgaggta gagcccggtg tcgttcccgt tcaccgacag cccccagccg tatcggatcc
     1681 ccgtcgagcc gagggcgggc atgtcggcga cgtcgaggcg gaacacgagc gtgctcccgt
     1741 tcggcgcgcg actgatcgtc gccttcgtga tgtcggcgcc gatgcccgcg ggcgtcgcgt
     1801 cgccggaggg gtcatccgcc gcggtgaagg gcacgtcgac gaacgtcgcc gtcccgctga
     1861 cgttgaggaa gctccccgaa acggttgccg agagcgaatc gacgtgcgtc gcggggccgg
     1921 ggtgggcggt cgctcccggc gcgagcgcca gggcagccac cagcacgagc ggtgtcgcga
     1981 tccggtgcac cttcatcatc cgtcctcctc ggggcgagcc ttcccgccgg cgctcacagc
     2041 tgcgaggccg gtcatgtctc gttgctggtt cgctgcgagc ggcccggaaa cctttgggca
     2101 ggtgcacaaa ggtggcggtc ggtccgccac cccccgaaga agcgccttgt gcacctgcac
     2161 aaaggggaag ccagcacgga cgcggaacac atggagggaa gatccgatcg aacggagggt
     2221 tcagatgaag cgcacgctcg ttctcgccgg cgcgctggca ctcggcctgc tgagcggtct
     2281 cacgccggcg tcggcggtct cgaccccggt cgcgacgcag gagaacgttc gccacctggc
     2341 gacggtcccc gggaacaccg gcggccacgt cgtggccgag gggaaccgcc tctatatggg
     2401 gaactacggc accggcctgt ccgcctacga catcagcgac ccccggaacc ccgtcaagat
     2461 cggccagtac ctgcccgggc cgtcagccac cgatggcgtg gacacgggcg ttcgcgcgga
     2521 cgcggtgccg gacgccgcca tctgggacgg ccggcacatc gtctcgctgg gcgggaccct
     2581 ccgcggaccg aaccgcgtgc agaccgagtt catcgacttc acggaccccg ccaacccgca
     2641 gctcctgcgc cggttcgccg gttcgaacct cagcggtggc gccgatggcg agtcgcacaa
     2701 cggcgacatc gtcgatgcgc ggaagctctt cctcccctcc ggaggcaccg gcaacaacgg
     2761 gctgcggatc tacgacatga ggccgctcct gaactcaccc gctggggcgc cggcgaggct
     2821 cgggggctgg aacccgcaca gcctctggca gaactcgccc taccgcatca actacgggaa
     2881 ggcgcccggc tccgtcttcg accacacgca cgacctcgag gtctacacgg acctgcgcgt
     2941 cctccttccc gagtgggagt gggaggacca ggacgaggac ggcgaacccg acccgaccta
     3001 cgggaacaag gacgtcgtct tgctcgccgc cgcgctgggc gggaacaacg gcggcgccgt
     3061 ctacgtgatc gacatcacga acccgagggc tccggtcgcc atgggcaagt gggagaacac
     3121 gagcggcaac acgatcgact acctccacga ggtccagttc ctccacggtg acccgtccac
     3181 gctggtggtc acggacgagc acatgaccgc gggctgcgcc gagggggccc tctacacggt
     3241 ccgcatctcg gagaacctgt cccacatgac gaagctgggt gagtggacga tcgacggcgg
     3301 ggcgcacgac accccgggta cctgcatggg gtcgcacgtg atcagctcac acgaccggca
     3361 cgtgttcatg ggcgcctaca ccgcagggct ccaggtgatc gacctgcgga acccggcgca
     3421 gcccaggcgc gccggccgct acatcgccga agggcagaac tcctgggggg cgctgtacca
     3481 ccagggtgtc gtctacaccg gcgacttcgg cggacgcggg ctcgacgtgt tcgagttcat
     3541 caaggacccg gtagcgaagg ccttcgtcaa ggccgggcac ccgcagaccg ccgggaacgg
     3601 cacgttcgac ccgatctacg gcgtctcgga ggagctcgcc aagtgcgctc cggacagcca
     3661 gctcaacggc gtcgacgggt tctgggtgag gatccccgag gacaagcgcg acggcacggc
     3721 cactttccga gcgctcggca gcggacctgc cggcctctac gacgtcgacg tgtggttcat
     3781 gaacgcgaac tgctcccagt acgtcgagcc gcatctggcc tcggagggga ccgacgagga
     3841 gggcctgatc cccgagggag ccgcgtacgc cgtggtgagc ctgtacatgg gctcgccgca
     3901 gtgggtctac gcgcagatcc tgtagcgacc cttctcgatc cttgaagggg gcccccggga
     3961 acgggggccc ccttcttctg tgcaggtgca caaagggttc gagcggcggg acggcgaagg
     4021 acaagcacga agctcgccga accggaatgg agggatcgtc catgcgccgt acgctgctgc
     4081 tcgccgcggc aacatcgctc gcgctcctcg ccgggctcac gcccgcgggt gctcaggggc
     4141 tgagcacccc gctcgcgacc caggagaacg tccggcacct cgcctcggtg cccggcaaca
     4201 ccggcggcca cgtcgtcgtc gaggggaacc gcctctacat ggggaactac ggaaccggac
     4261 tctccgtcta cgacatctcc aacgttcgcg ccccggtcaa gatcggccag tacctgcccg
     4321 ggccgtcgac gaacgacggg gtcgaccccg gagcgcgcgc cgacgcggtt cccgacgcgg
     4381 ccgtctggga caaccggcac atcgtgtcgc tgaacggcac ctcccgcgcc tccaccacga
     4441 cccagaccga gttcatcgac ttcaccgatc ctgcgaagcc tgttctgctg caccgcttcc
     4501 gcggctcgtc tgacggcgag gcgcacaacg gcgacttcgt cgacgcgagg aggctgtggc
     4561 tcccgtcggg cggcagcggc aacaacggac tccggatcta cgacgtgaac ccggtgctcg
     4621 agagcccggc gaaggcgccg acgcgcctcg gcggctggaa cccacacacg ctctggcaga
     4681 a
//
LOCUS       DATLGG010000085         6386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_209149, whole genome shotgun sequence.
ACCESSION   DATLGG010000085 DATLGG010000000
VERSION     DATLGG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209149"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="VM638_02385"
     CDS             <1..2112
                     /locus_tag="VM638_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVM11312.1"
                     /translation="RLSAAALSLKLPIALVIASVVAVVVTSYRQTVRAYPQGGGSYIV
                     ARENLGRMPGLLAAAAILTDYVLTVTVSVTAGTIAVTSALPELADQKVLLALSFIAVL
                     SLANLRGAKEAGTLFAIPTYGFVAMIYVMLVTGFVRCLDACPQAATANVHLEETSALT
                     LFLILRAFSTGSAALTGIEAVADGVQAFRRPQGKNAANTLAIMGTMSITMFLGITALA
                     QVLHVRTTEELSKHKSVLAQIGETIFGGDSAMFFALQAFTALILILAANTAYQDFPRL
                     SAILARDRFMPSQFRNRGDRLVFSNGILVLSVAAGSLVWAFGAELTALIKLYVVGVFT
                     AFTLSQWGMVRRWFSRKEGAWRRGAAINATGGTVTGVVLIVVIVTKFTEGAWIVLIAM
                     PVIISGLLAVNRHYTRVQEQLRARNLSIDVEVRNTVVLLVPNLDLATQDAVAWLRAVR
                     PERVIPLYLGSEPTERLAPAWNAMAPRLGDLEPLRVGRGGLARAVRDVVRRIEREPED
                     YVTIVVPETIARKSLWHVFRNRTTLLKARLLFERRVAVVDSPLLPEEHDRHRGHGGGA
                     VLEPERTVCIVPVSAVHDATARALIFAKSLRPGRLEAVFFAGERAEADEIIADWRERG
                     FDLPLTVAESPFRDITASVLAEVRRHTADGRTLVTVVLPEFVVKKWWHHLLHNQTALT
                     LKRVLLFEPRTVVASVPSHVD"
     gene            complement(2116..5559)
                     /locus_tag="VM638_02390"
     CDS             complement(2116..5559)
                     /locus_tag="VM638_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVM11313.1"
                     /translation="MRPLLTLVIGFALLGAPAVAQEPNDPPDDPIVFAPRGLSADRFP
                     AATSPQWVDVTAHDGVKLAARVYRPDTSSDPGWKTPVILVHSPYYGAQSSPGSRSMDL
                     VARYTPKGYTVVLSSVRGTGDSGGCLEQDGPNQARDFKTLVEHFARQPWSNGKVGSYG
                     KSYDAEAQNAGAILRPEGLETMVTVAGISSLYDVGYFDGVPFTLGGLTASAGYATTGP
                     GVLPTEGSRYLERANCYPDVYGNSLDPSGDETPFWAERNFRYGVRDVEASVLFVHGLN
                     DFTVNPLAIDGWYDELPVFKRAIFGQWAHWYPYDAPSAVARNDWYDTVDAWFDHELLG
                     LETGIEAWPPVQVQDEQNVWRAVRSFRGMGAERTLPLGTGTLGTAGGSGETVRYEERT
                     PASWDGPTLDAPLHLSGQAFLDATIAIDRPDAHLAVTLSEVRANGTVRTLTRGYLSVQ
                     HREDPNRGHEVTPGHPIPYRVRTFPFDKTVAAGSKLRLALGGSDNSTRAAGTAYTGHV
                     HVDGRSVLRIPVVEERCGLVVARRAPGPPPPLCVDQVIPAETPRHVPDAANGHRATAR
                     LISSAAETVGGVPALRETGYLTVRDGIELAFEVVRPAAAGERPVLLTYDAYDAGRNPD
                     PGYAARYVPRGYSLVGLNVRGTGCSGGVYDFFEPAEGIDGFEMVEWIARQPWSNGRVG
                     MIGKSHPGISQLFVAETRPPHLAAIAPGHHFMDVYRDVAFPGGILNYAFGGGWSLVQP
                     TYGLQSLPSDIAAGDETCIRNLSKHGRNARTNAFVQAQEHPYDGPFLQNRSPARNLHR
                     IEVPVFTAQSWQDHQLGSRQVHMLDRLEELGIEYRAVLSNGDHDMYRRGPQMAQLDRF
                     LEAHVERRDVLRDGTPREAYLAEPPVTVLWEQAGNQARWSTTLDDWGKARPTTLYVNG
                     PGRLRSEAPGAGAPPQEYMHTALGSQGNANLQHGNASFPNVSWWDKHAPPEGTALALT
                     ADPAQDDTVLLGSASADLWITSSAPNVDLQVTLTEVRPDGQEVYIQQGWLRTKQRALD
                     RHASTTLLPVQTHRQADVRPLSATEPSLARVEILPFGHVIRKGSRLRMWIEAPTVMPQ
                     LWGFALDPTPARVSIHQDAAHPSRLVLPVAPDISLPDAAKAHGACGQQIRQPCRTDPR
                     G"
     gene            5646..6194
                     /locus_tag="VM638_02395"
     CDS             5646..6194
                     /locus_tag="VM638_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HVM11314.1"
                     /translation="MEREDALALVRDKTEKETTVRHLISVEGVMRALARRLGEDEETW
                     GLVGLFHDLDQDRTGDDGEGHAHLAAGWLREAGVDEAIVNGVLAHAYPEYRTDRMSQA
                     IVHADAVAGLLVAAALVRPQKANGMKVSSVKKKLKERAFAPGVKREEITGVEEGIGLP
                     LDDFIATSIEGLQQVAPEIDLA"
     gene            complement(6198..>6386)
                     /locus_tag="VM638_02400"
     CDS             complement(6198..>6386)
                     /locus_tag="VM638_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVM11315.1"
                     /translation="FLGCLPWSFALAWGGLVLGDNWETVLRYGEPISWGIAVLVAAVI
                     GWWIWKRVRERRAERASS"
BASE COUNT          896 a         2207 c         2305 g          978 t
ORIGIN      
        1 aggctgagcg cggccgcgct cagcctcaag ctgccgatcg cactggtgat cgcgtccgtg
       61 gtggctgtcg tggtgacctc gtaccggcag acggttcggg cgtatccgca gggaggcggc
      121 tcctacatcg tcgctcgcga gaacctcggc cggatgcccg gactgcttgc ggccgccgcc
      181 atcctgaccg actacgtcct gacggtcacg gtgagcgtca ccgccggcac gatcgccgtg
      241 acgtcggctc tgccggagct cgcggaccag aaggtgctcc tggcgctgag cttcatcgcc
      301 gttctctcac ttgcgaacct gcgtggggcg aaggaggccg gaaccctgtt cgcgataccc
      361 acatacgggt tcgtcgcgat gatctacgtc atgctcgtga ccggattcgt ccgatgcctg
      421 gatgcctgcc cgcaggccgc taccgccaac gtgcacctcg aggagacgag cgcgctcacg
      481 ctgttcctga tcctgcgggc tttctcgacc ggctcggccg ccctcaccgg gatcgaagcc
      541 gtcgccgacg gtgtgcaggc gttccgtcgg ccacagggga agaacgccgc gaacaccctc
      601 gcgatcatgg ggacgatgtc gatcacgatg ttcctcggca tcacggcgct cgcgcaggtg
      661 ctccacgtgc ggacgaccga ggagctctcg aagcacaagt cggtgctggc gcagatcggg
      721 gagacgatct tcggtggtga cagtgcgatg ttcttcgcgc ttcaggcgtt cacggcactc
      781 atcctgatcc tcgccgcgaa caccgcgtat caggatttcc cgcgcctctc ggcgatcctg
      841 gcacgggacc gcttcatgcc gagtcagttc cgcaaccgcg gcgaccgact cgtgttctcg
      901 aacggcatcc tcgtcctctc ggtcgctgcc ggatcgctcg tctgggcctt cggcgctgag
      961 ctcaccgcgt tgatcaagct ctacgtcgtc ggcgtcttca cggcgttcac cctttcgcag
     1021 tggggcatgg tccgtcgatg gttcagccgg aaggaagggg cgtggcggcg tggggccgct
     1081 atcaacgcga ccggcggcac cgtcaccggc gtcgtcctca tcgtcgtgat cgtcacgaag
     1141 ttcacggaag gcgcatggat cgtgttgatc gcgatgccgg tgatcatctc cgggcttcta
     1201 gccgtgaacc gtcactacac gcgggtccag gagcagttgc gggctcggaa cctctcgatc
     1261 gacgtggagg tgcggaacac cgtggttctg ctggtgccga acctggacct ggcgacgcag
     1321 gacgccgtcg catggctccg cgccgtccgc ccggagcgcg tgatcccgct ctacctcgga
     1381 tccgagccga cggagcgcct cgcgcccgcc tggaacgcga tggccccgcg cctcggtgat
     1441 ctcgagccgc tgcgggtcgg gcgcggcggc ctggcgcgcg cggtccgcga cgtcgtccgc
     1501 aggatcgagc gcgagccgga ggattacgtg acgatcgtcg tgccggagac gatcgcgcgg
     1561 aagtcgctct ggcacgtctt ccggaaccgc acgacgctcc tcaaggcacg cctcctgttc
     1621 gagcggcgcg tcgcggtcgt ggacagtccg ctgctgccgg aggagcacga ccggcaccgc
     1681 ggccatggtg gcggggcggt gctcgagccg gagcgcaccg tctgcatcgt ccccgtctcc
     1741 gcggtccacg acgcgacggc acgcgcgctc atcttcgcca agtccctgcg cccggggcgg
     1801 ctcgaggcgg tcttcttcgc cggggagcgg gcagaggccg atgagatcat cgcggactgg
     1861 cgcgagcgcg ggttcgacct tcccctcacg gtcgccgagt cgccgttccg cgacatcacc
     1921 gcctcggtcc tcgcggaggt ccgtcgccac acggccgatg gccgaaccct cgtgacggtc
     1981 gtcctgcccg agttcgtcgt gaagaagtgg tggcaccacc tcctgcacaa ccagaccgcg
     2041 ctcacgctca agcgcgtgct gctcttcgaa ccgcggacgg tcgtggcgag cgtgccgtcg
     2101 cacgtcgact gagcgctacc cccgggggtc ggtccggcag ggctggcgga tctgctgccc
     2161 gcaggccccg tgcgccttgg ccgcgtccgg cagggagatg tccggggcga ccgggaggac
     2221 gagtctcgac gggtgcgccg cgtcctggtg gatcgagacg cgcgcggggg tggggtccag
     2281 ggcgaacccc cagagctgcg gcatgaccgt cggggcctcg atccacatcc gcaggcgcga
     2341 gcccttgcgg atcacgtgtc cgaacggaag gatctccacc cgcgcgagcg acggctcggt
     2401 cgcggagagc ggacggacgt cggcctgccg gtgggtctgg accggcagga gcgtggtcga
     2461 cgcatggcgg tcgagcgccc gttgcttcgt ccggagccat ccctgctgga tgtagacctc
     2521 ctgcccgtcg gggcggacct cggtgagcgt cacctgcaga tcgacgttgg gcgcactcga
     2581 ggtgatccac aggtcggcgg aggccgagcc cagcaggacg gtgtcgtcct gcgccgggtc
     2641 ggcggtcagc gcgagcgcgg tcccttcggg gggtgcgtgc ttgtcccacc aggagacgtt
     2701 cgggaacgac gcgttcccgt gctggaggtt cgcgttgccc tggctgccga gcgcggtgtg
     2761 catgtactcc tgcggtggcg cgccggcacc cggcgcctcg gaccgcagcc gaccgggacc
     2821 gttcacgtag agcgtggtcg gtcgcgcctt cccccagtca tcgagggtgg tcgaccagcg
     2881 ggcctggttc cccgcctgct cccacagcac ggtgaccggt ggctctgcca ggtacgcctc
     2941 gcgcggggtc ccgtcgcgca gcacgtcgcg tcgctcgacg tgcgcctcga ggaaccgatc
     3001 gagctgcgcc atctgcgggc cgcggcggta catgtcgtgg tcgccgttcg acaggaccgc
     3061 gcggtactcg atgccgagct cctcgagccg gtcgagcatg tggacctgcc gcgacccgag
     3121 ctggtggtcc tgccacgact gcgcggtgaa cacggggacc tcgatccggt ggaggttccg
     3181 tgccgggctc cggttctgca ggaacggccc gtcgtacggg tgctcttggg cctggacgaa
     3241 agcgttcgtc cgcgcgttgc gcccgtgctt cgagaggttg cggatgcagg tctcgtcgcc
     3301 cgcagcgatg tcactcggca ggctctgcag gccgtacgtc ggctgcacga gcgaccatcc
     3361 gccgccgaac gcgtagttga ggatcccgcc cgggaaggcg acgtcgcggt agacgtccat
     3421 gaagtgatgg cccggggcga tcgcggcgag gtgcggcggg cgcgtctcgg ccacgaagag
     3481 ctggctgatc ccggggtgcg acttcccgat catgccgacc cggccgttcg accacggctg
     3541 gcgtgcgatc cactcgacca tctcgaagcc gtcgatgcct tccgccggct cgaagaagtc
     3601 gtagacgcca ccggagcagc cggtgccgcg gacgttgagc ccgacgaggg agtacccgcg
     3661 gggcacgtac cgcgcggcgt accccggatc ggggttgcgg ccggcgtcgt aggcgtcgta
     3721 ggtgagcagg acgggccgct cgccggcggc cgcgggccgg acgacctcga aggccagctc
     3781 gatcccgtca cgcacggtga gatagccggt ctcgcggagc gccggcacgc cgccgaccgt
     3841 ttcggctgcc gaggagatca gccgcgccgt cgcccggtgg ccgttcgcgg cgtccggcac
     3901 gtgccgcggt gtctccgccg ggatcacctg gtccacgcac aacgggggtg gcggacccgg
     3961 cgcgcgccgc gccacgacca ggccgcatcg ctcctcgacg acggggatgc gcaggaccga
     4021 gcggccgtcc acgtggacgt gaccggtgta ggcggttccg gcggcacgcg tggagttgtc
     4081 ggagccgccg agcgcgaggc ggagcttcga ccccgcggcc acggtcttgt cgaaggggaa
     4141 cgtccgcacg cgatacggga tcggatggcc cggcgtcacc tcgtggccgc ggttcgggtc
     4201 ctcccggtgc tggaccgaga ggtagccgcg cgtgagggtc cggacggtgc cgttcgcacg
     4261 gacctccgac agggtgacgg cgaggtgcgc gtcgggccgg tcgatcgcga tcgtcgcgtc
     4321 gaggaacgcc tgtcccgaca ggtgcagggg cgcgtcgagc gtggggccgt cccagctcgc
     4381 gggcgtccgc tcctcgtacc gcacggtctc gccggagcca cccgcggtgc cgagggtgcc
     4441 ggtcccgagc gggagcgtgc gctcggcccc catcccgcgg aacgagcgga cggcgcgcca
     4501 gacgttctgc tcgtcttgaa cctgcaccgg cggccacgcc tcgatcccgg tctcgagccc
     4561 gaggagctcg tggtcgaacc aggcgtcgac ggtgtcgtac cagtcgttgc gcgcgaccgc
     4621 gctcggcgcg tcgtacgggt accagtgggc ccactgcccg aagatcgcgc gcttgaacac
     4681 gggcagctcg tcgtaccagc catcgatcgc gagggggttc accgtgaagt cgttcagccc
     4741 gtggacgaag agcacgctcg cctccacgtc gcggacgccg tagcggaagt tccgctccgc
     4801 ccagaacggc gtctcgtcgc cgctcgggtc gagcgagttc ccgtagacgt ccgggtagca
     4861 gttcgcccgc tcgaggtagc ggctcccttc ggtcgggagg acccccgggc cggtcgtcgc
     4921 gtacccggcg gacgcggtga ggccgccgag ggtgaacggg acgccgtcga agtacccgac
     4981 gtcgtagagg ctcgagatgc cggcgaccgt gaccatcgtc tccaacccct cgggccgcag
     5041 gatcgcgccg gcgttctgcg cctcggcgtc gtaggacttg ccgtacgagc cgaccttccc
     5101 gttcgaccac ggctggcgag cgaagtgctc gaccagggtc ttgaagtccc gcgcttggtt
     5161 cggcccgtcc tgctcgaggc accccccgga gtcgccggtg ccgcggacgc tcgagagcac
     5221 gaccgtgtac cccttcgggg tgtagcgcgc gacgaggtcc atcgagcggg acccggggct
     5281 cgactgcgcg ccgtagtacg gcgagtgcac gaggatcacc ggggtcttcc aaccggggtc
     5341 gctcgaggta tcggggcgat acacgcgggc cgccagcttg acgccgtcgt gggccgtgac
     5401 gtccacccac tgcgggctcg tcgccgccgg gaaccggtcc gcgctcaggc cgcgtggagc
     5461 gaagacgatc gggtcgtcgg gcgggtcgtt cggctcctgg gcaaccgcgg gcgcgccgag
     5521 cagggcgaag ccgatgacga gggtgaggag cggccgcatc acccgtatct gttcgccacc
     5581 ggggaccggt cctccttcgt gcacctgcac aacggggccg ccaccccccc ggctaggatg
     5641 accttatgga acgggaggat gccctcgcgc tcgtccgtga caagaccgag aaggagacga
     5701 ccgtccggca cctcatcagc gtcgaggggg tgatgcgggc gctcgcgcgg cgcctcggcg
     5761 aggacgagga gacctggggg ctcgtcgggc tcttccacga cctcgaccag gaccggaccg
     5821 gcgacgacgg cgagggccat gcccatctgg cggccggatg gctgcgagag gcgggcgtcg
     5881 acgaggcgat cgtgaacggg gtcctcgccc acgcctatcc cgagtaccgc accgaccgga
     5941 tgtcgcaggc gatcgtccac gccgacgccg tcgccggcct cctcgtcgcg gccgcgctcg
     6001 tccgtcccca gaaggcgaac ggcatgaagg tctcgagcgt gaagaagaag ctgaaggagc
     6061 gggcgttcgc gccgggcgtg aagcgcgagg agatcacggg ggtcgaggag gggatcggcc
     6121 tgccgctcga cgacttcatc gcgacgagca tcgagggtct ccagcaggtc gcccccgaga
     6181 tcgacctggc gtgacggtca ggagctcgcg cgctcggccc gccgttcgcg gacgcgcttc
     6241 cagatccacc agccgatcac ggccgcgacc agcaccgcga tcccccagct gatcggctcg
     6301 ccgtagcgga gcaccgtctc ccagttgtcg cccaggacga ggccacccca ggcgagcgcg
     6361 aacgaccacg gcaggcaccc gaggaa
//
LOCUS       DATLGG010000086         3567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_66893, whole genome shotgun sequence.
ACCESSION   DATLGG010000086 DATLGG010000000
VERSION     DATLGG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66893"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..381
                     /locus_tag="VM638_02405"
                     /pseudo
     CDS             70..381
                     /locus_tag="VM638_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
     gene            391..1050
                     /locus_tag="VM638_02410"
     CDS             391..1050
                     /locus_tag="VM638_02410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HVM11316.1"
                     /translation="MNEVRGRHVDDPERRAARRLTRRLEALGVRVGGRAGAGTPPSGL
                     VRVAGIRGPTVATVLRPVNTGSWNFGAEVLGKRLGQERAGAPGTIAKGATATEAWASA
                     AGVGVDANDASGLSYRNRVTAAGVARLLGVAEDTPWGPALRTTLASPGSGTLRERLGG
                     VRVRAKTGTLRGVSALSGWVWLERRGAWGEFSIISSGLSKARAVAIEDAAVRLLAARG
                     R"
     gene            1086..1376
                     /gene="clpS"
                     /locus_tag="VM638_02415"
     CDS             1086..1376
                     /gene="clpS"
                     /locus_tag="VM638_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HVM11317.1"
                     /translation="MAGTAPVEVDRPSGEDVVVPDRVWKVIVWNDPINLMSYVTFVFQ
                     KLFGYSRAKATKLMLDVHHKGKAVVSSGNREKAELDTFRLHQHGLWATMQQD"
     gene            1379..1831
                     /locus_tag="VM638_02420"
     CDS             1379..1831
                     /locus_tag="VM638_02420"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HVM11318.1"
                     /translation="MRRHIERAGRGTFRLRLSEQERDVLRRLPRQLRSLLDRRDPSVW
                     RLFPPAYDEEESEAEYRRLMGEELVAAKHEALRIVEETIDAKRLDEDQLAAWLGAIND
                     LRLVFGTRLGVTEELDEEEMLQGPDGATYSVYLFLGWLEEQAVAALSG"
     gene            complement(1835..2740)
                     /locus_tag="VM638_02425"
     CDS             complement(1835..2740)
                     /locus_tag="VM638_02425"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVM11319.1"
                     /translation="MDHPALLYSSLPRFVDEVVPFVREGIERGEPVVVAVGDSELAAL
                     RKELGPDRPGMELADTRRWHPHPASRLRAFHGIALEAMEQGARRVRLVGEPAWPTGPP
                     EFVQEWERYESVLNRVLGPLPVTLICTYDTTRTAPAILDHAQVTHPTVDGDPSARYVR
                     PDEVLARWNDPLSDPPEDADLLERPASLPEVRRFVRDAASRAGVAPDRVTDLCIAANE
                     VVTNAEIHGGEPITLRVWTDGDRFLCQVDDAGKRTIDPLAGYLPPSGRGDSGRGLWLA
                     RQLVDLLQVVPRPGGTSVRLHVFRA"
     gene            2982..>3567
                     /locus_tag="VM638_02430"
     CDS             2982..>3567
                     /locus_tag="VM638_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11320.1"
                     /translation="MRARRTIRTLVSTLVLLPAAVGASAGDPSVTILQPTAGATHSKA
                     AGPIAVSGAVGFEQPAPADTRLFLRYAAEGGTNCGRRYLSTTNGPDPGNSCQSTGAHL
                     TLAGQSFTRDWPADPAEVALPLTLDASRNITGEININSRFLGTAGAPLASPGNWVTLD
                     VVVRLGDTALTQSFDSGLYTGATRKFSLNLDIPAS"
BASE COUNT          499 a         1319 c         1268 g          481 t
ORIGIN      
        1 atctcggtcc gggacgccgg gatcctgccg agggtgtcgc ggcgacggta gaggaagctc
       61 ccggcgtcga accagaagct cctgatgtcg atggcgctcc tcgaccgcct cgatcccaac
      121 acccggcttc ccacgaccgc gttcgccggc gccgtcggga acggggggac ggtcgccggg
      181 gatctgtgga tcgtcgggag cggcgacccg accgtcggac ggcgcgagat cgatcggctc
      241 gcgcgtcgga tccgcgacgc cggggtcacg aggatccgcg gatcggtgcg gggcgcgacg
      301 cggccgttcg cgcgtgactg gttcgccccc ggctggagag cggacttccc ctcacgcgag
      361 atcgcgctcc cgaccgcgct cacctacgag ctgaacgagg tccggggccg acacgtcgac
      421 gatcccgagc gccgcgcggc ccgccggctc acccgtcgcc tcgaggcgct cggcgtccgg
      481 gtcggcggca gggccggggc ggggacgccc ccctcgggac tcgtccgcgt cgccggcatc
      541 cgcggcccga cggtcgccac cgtcctgcgg cccgtgaaca ccggatcgtg gaacttcggc
      601 gccgaggtcc tcgggaagcg cctcgggcag gagcgggccg gcgcccccgg cacgatcgcg
      661 aagggtgcga cggcgaccga agcgtgggcc tcggccgcgg gggtgggggt cgacgcgaac
      721 gacgcgtccg ggctctcgta ccggaaccgg gtcaccgccg cgggcgtcgc gcgcctcctc
      781 ggggtcgccg aggacacacc gtgggggccg gccctgcgga ccaccctcgc ctcacccggg
      841 tccgggaccc tgcgggagcg cctcggtggc gtccgggtac gtgcgaagac cgggaccctg
      901 cggggggtct ccgcgctctc ggggtgggtc tggctcgagc gccgaggagc ctggggcgag
      961 ttctcgatca tctcctcggg tctctcgaag gcgcgggcgg tcgcgatcga ggatgcggcg
     1021 gtgcgcctcc tggctgcgcg cggccggtag ccccgccgac ccccctcgtc tgcgacaatc
     1081 gacgcatggc cggcaccgct cccgtcgagg tcgaccgtcc ctcgggcgag gacgtcgtcg
     1141 ttccggaccg cgtctggaag gtcatcgtgt ggaacgaccc catcaacctg atgtcgtacg
     1201 tcaccttcgt cttccagaag ctgttcggat acagccgcgc gaaggcgacg aagctcatgc
     1261 tcgacgtcca ccacaagggc aaggcggtcg tctccagcgg gaaccgggag aaagcggagc
     1321 tcgacacctt ccgcctgcac cagcacggtc tgtgggcgac gatgcagcag gactgatcgt
     1381 gcggcgccac atcgaacggg cggggcgggg gacgttccgg ctgcgcctga gcgagcagga
     1441 gcgggacgtc ctccggcgtc tcccgcggca gctccggtcg ctcctcgacc gccgggaccc
     1501 atccgtctgg cgcctgttcc cgcccgcgta cgacgaggag gagtccgaag ccgagtatcg
     1561 ccgcctgatg ggcgaggagc tcgtcgcggc gaagcacgag gccctgcgga tcgtcgagga
     1621 gacgatcgac gcgaagcgcc tcgacgagga tcagctcgcg gcgtggctcg gcgcgatcaa
     1681 cgatctccgt ctggtgttcg gcacccggct gggcgtgacc gaggagctcg acgaggagga
     1741 gatgctccag gggccggacg gcgccacgta ctcggtgtac ctgttcctcg gctggctcga
     1801 ggaacaggcc gtcgccgcgc tctcggggtg agcctcaggc gcggaagacg tggaggcgga
     1861 cgctcgtgcc gcccggtcgc gggacgacct ggaggagatc gacgagctgc cgcgcgagcc
     1921 agagcccgcg gccggagtcg ccgcgtccgc tcggggggag gtagcccgcc agggggtcga
     1981 tcgtccgctt cccggcgtcg tcgacctgac acaggaaccg gtcaccatcg gtccagaccc
     2041 ggagcgtgat cggctccccg ccgtggatct ccgcgttcgt gacgacctcg ttcgccgcga
     2101 tgcacaggtc ggtgacccgg tccggcgcca cgccggcccg gctggccgcg tcccgcacga
     2161 accgccggac ctcggggagg ctcgcgggcc gctcgagcag atcggcgtct tccggcggat
     2221 cgctcagggg gtcgttccag cgggcgagca cctcgtccgg ccgcacgtac cgggcgctcg
     2281 ggtcgccgtc gaccgtgggg tgggtgacct gcgcgtggtc gaggatcgcg ggagccgtcc
     2341 gcgtcgtgtc gtaggtgcag atcagggtga ccggaagcgg tccgaggacg cggttgagga
     2401 cggactcgta ccgctcccac tcctgcacga actcgggagg tccggtcggc cacgcgggct
     2461 cgccgacgag ccggacgcga cgagccccct gctccatcgc ctcgagggcg atcccgtgga
     2521 acgcgcggag gcgcgacgcc ggatgcgggt gccaccgccg ggtgtccgcc agctccatcc
     2581 ccggacggtc ggggccgagc tccttccgca gcgccgcgag ctcgctgtcc cccacggcga
     2641 cgacgaccgg ctcgccccgc tcgatcccct cccgcacgaa ggggacgacc tcatcgacga
     2701 agcgcggcag gctcgaatag agcagagccg gatgatccat tcgtccccgc gacgaggcgc
     2761 gcgcgctatc gaccgccatc ggtctccctg cctcccacgg ggtccgagtg tgccagccgg
     2821 ccgtcgatgc tcctaccccc caggatgacc atctgaagca tcccccgaac gggctgccgg
     2881 aacccccgcc ccggaagtct gtgcagctgc acaagcggcc aggcagggat atcagccctc
     2941 cctgaagaac cttgcaccga cgtctcgcca ggaggtctac catgcgcgct cgccgaacga
     3001 tccgaacgct cgtctcgacc ctcgtcctcc tccccgccgc cgtcggcgca tcagccgggg
     3061 acccgtccgt caccatcctc cagccgacgg ccggagcgac ccacagcaag gccgccgggc
     3121 cgatcgcggt ctccggcgcc gtcggtttcg agcagccggc cccggccgac acgcgtctct
     3181 tcctgcgcta cgcggcagag ggtgggacga actgcggccg gcggtacctc tcgaccacga
     3241 acgggccgga cccgggcaac agctgccagt cgacgggtgc gcacctcacg ctcgcggggc
     3301 agagcttcac ccgcgactgg cccgccgacc ccgcagaggt cgcgctgccg ctgaccctcg
     3361 acgcctcccg caacatcacc ggcgagatca atatcaacag ccggttcctc gggacggccg
     3421 gcgcccccct ggcttccccg ggcaactggg tgacccttga cgtcgtcgtc cgcctcggcg
     3481 acaccgccct gacgcagtcg ttcgacagcg gcctctacac cggcgccacg cgcaagttct
     3541 ccctgaacct cgacatccct gcttcgc
//
LOCUS       DATLGG010000087         9301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_3118, whole genome shotgun sequence.
ACCESSION   DATLGG010000087 DATLGG010000000
VERSION     DATLGG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VM638_02435"
     CDS             complement(<1..1708)
                     /locus_tag="VM638_02435"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HVM11321.1"
                     /translation="MEQGSVPRIAEAAPPDRRGGLWARLPFRSGSPKPPAQLEALLRT
                     VRTYNAKAELPVIRKAYDVAERSHRGQVRKSGDAFITHPLGVAQILADLGMDATTVIA
                     ALLHDVVEDTAVTVEDIREEFGDQVALIVDGLTKLDRIEFRTKEHQQVENVRKMIIAM
                     ARDIRVLIIKLADRLHNMRTLGALSREKQTEKATETLEIYAPLAHRLGIFQIKWELED
                     LAFATLHPRRFTEISNLVGKAEDEREAYLREVVETVGETLKKLKVKHEIEGRPKHLYS
                     VYEKMVLRGKEFAEIYDLVGIRVLVDEVRDCYAALGAIHSVWKPVPGRFKDYIAMPKF
                     NMYQSLHTTVIGPEGRPLEVQIRTWDMHRTAEYGIAAHWRYKEGAKKKDEAEMAWLAQ
                     MLDWQKEATDPRDFMEGLKIDLYADQVFVFTPKGDVMNLPAGATPIDFAYAIHTEVGH
                     RCIGAKVNGRLVPLDYELQTGDTAEVITSKAQEAGPTRDWLSIVKSPRARTKIRQWFS
                     RERREDAMDAGRELLQRELRRQGLPLKRLTTEELLARVAVELNFSGLDSMLVGIGEGH
                     VSP"
     gene            complement(1728..2252)
                     /locus_tag="VM638_02440"
     CDS             complement(1728..2252)
                     /locus_tag="VM638_02440"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HVM11322.1"
                     /translation="MNDLERLRGHIRDIPDFPKPGIVFKDITPLLADPVAFSTVIDVI
                     VVHYGRGNVDKVVGIEARGFIMAAPVAYHFGAGFVPVRKKGKLPWETEAAEYDLEYGT
                     ETLEIHKDAFRPGERVLIVDDVLATGGTARATADLVERLGGKVVGIACVIELSFLDGR
                     RAIEGYDFTSLLTY"
     gene            complement(2262..3437)
                     /locus_tag="VM638_02445"
     CDS             complement(2262..3437)
                     /locus_tag="VM638_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVM11323.1"
                     /translation="MPSAVLLLELGLVILALAAVSRLAHRFGFSPIPLYLLAGLAVGE
                     GGLVPIVTAEDFIEVGAEIGVILLLLMLGLEYSAEELTSSLRRSAPVGALDLVLNFSP
                     GFLLALVLGWGWLAALFLGGVTYISSSGIVAKQVSDLGWVGNRETPAVLSVLVIEDLV
                     MAAYLPFTAALLQGGGLARLSLTVVIAVGSAILILALALRHGQALSRAVFSHSDETLL
                     LSIVGITLLVAGLAERLHISAAVGAFLVGIALSGRAADRARGVLTPLRDLFAAVFFVF
                     FGLRVDPSSIPQVAGMALVLAFVSGVTKVWTGWVAARREGAAPRGRLRAGMLLIARGE
                     FSLVIAGLAVAAQLHPDLGSLAAAYVLFMAALGPVAVRLLPGVALRRPTATAAVRGE"
     gene            complement(3441..3923)
                     /locus_tag="VM638_02450"
     CDS             complement(3441..3923)
                     /locus_tag="VM638_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HVM11324.1"
                     /translation="MAKIEETVLPGMGIRYAFLTEAGKRVGVLHHRTGRRELLLYDRD
                     DPDMCAEVLALEDDDARTLIEVLGGSRVAEQLSTIQRIEGLAIDWLPLRDDTPYAGRT
                     IGDTAARTRTGTSIVAVVRGADAHPAPGPDFGLEAGDTLVVVGTPRGIEDLSVLLRTG
                     "
     gene            complement(3961..5061)
                     /gene="secF"
                     /locus_tag="VM638_02455"
     CDS             complement(3961..5061)
                     /gene="secF"
                     /locus_tag="VM638_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVM11325.1"
                     /translation="MSLRSAIATFRGHRQFHMDIIGRRRLWFAISGLLVVLSIGSLFI
                     RGLNLGIDFRGGTQLVYPNPAGVSTTEVRETMARFGYGDSVVQVVDDSEVSVRTGDLG
                     DRRADVTAALAEQAGIDAGQVNAQVIGPRWGEQISRKALQGLIIFLVVVSIYIAMRFE
                     WKMSVAAITALFHDLIITTGIYSLSGREVTPETVIAILTILGYSLYDTVVIFDKIRDN
                     AASPSLVAKETYAGAVNLSLNQVFMRSVNTSITTLLPIGTLLLFGGETLQNFAFALFV
                     GVALGAYSSIFVAAPLLTTLKEREPRLAQLRERARARRPELAAVPGGADVVEADVVEA
                     PAPRSGSGSRGATPAARPRRPKKKPRAKRKKR"
     gene            complement(5058..6566)
                     /gene="secD"
                     /locus_tag="VM638_02460"
     CDS             complement(5058..6566)
                     /gene="secD"
                     /locus_tag="VM638_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVM11326.1"
                     /translation="MRGGGRLAASALIMLAIAGAAIWGFSGANLRPQLGLDLVGGVSL
                     TLTAPPGTDSEVVDKTLEVIRNRVDQFGVAEPDITRQGELNILVQIPQAETGSQERLL
                     ELIGQTARLEFRIVEEAVAPGTPEYDELQVTAEGEDGATEPSAAPTPAPSPGAEEETA
                     DEQQQEQEEEAEGEVVYLGSGEDRTKYRLGPVQMTGEALRDARAVFNDPAAVTDPGSS
                     GWVVSFRLNGEGAETFARITRENTNRQLAIVLDERVESAPNINEEIAGGEGQISGNFQ
                     EQEAKDLALVLRTGALPIELQREELRTVSPTLGRESLRQGLLAGVAGLIALAIYLFFY
                     YRVLGVVTWFGMAIWATLTLAIVSLLGRTVGYSLTLAGVAGLVVSVGVAADSYVVFYE
                     RLKDEVRRGKTLRAAVGPAFTSAWRTIVAANLVTIAAAVILYLVAVGAVRGFALTLGL
                     ATFLDLIVMFFFKRPTVFLIARSRRLSEMRGMGLRSGVAADPDPVPVRGGSR"
     gene            complement(6563..7240)
                     /locus_tag="VM638_02465"
     CDS             complement(6563..7240)
                     /locus_tag="VM638_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVM11327.1"
                     /translation="MGDPASAGLSFDDVKQDPVISTFIAQADRVMDGLGFTEHGFRHA
                     NLTGSIARNLLERLGHDERTVTLAAIGGYLHDIGNLVSRAVHGQTGAILVQQILADRM
                     DPQDLAIVMGAVGNHEEEHGMTAGPVAAAVILADKADVHRSRVRKDDRIEFDIHDRVN
                     YAAERSFLRVDSAAKTVSLELTIDTQISDIMEYFEIFLGRMIMCRRAAEELDCGFHLE
                     INGAKLL"
     gene            complement(7246..7581)
                     /gene="yajC"
                     /locus_tag="VM638_02470"
     CDS             complement(7246..7581)
                     /gene="yajC"
                     /locus_tag="VM638_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVM11328.1"
                     /translation="MIATLLAQAQTQQPRGNATFLISLGLMVAIFYFLLIRPQQRRVR
                     QQRDLVSALQVGDEVVTIGGMYGRIRGMSDDEVSLEISPGTEVRFVKQAIARRLVPEP
                     LEGEGSGGA"
     gene            complement(7668..7880)
                     /locus_tag="VM638_02475"
     CDS             complement(7668..7880)
                     /locus_tag="VM638_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HVM11329.1"
                     /translation="MDAFGRVLLVVAAVLALVGLVLVAVGKGLIPRLPGDLTFGRGNV
                     RVYLPLGTMLLVSIVLTLVVNLFLRR"
     gene            complement(7890..8900)
                     /gene="ruvB"
                     /locus_tag="VM638_02480"
     CDS             complement(7890..8900)
                     /gene="ruvB"
                     /locus_tag="VM638_02480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVM11330.1"
                     /translation="MSAERIVTPDELELDRELDVALRPKRLEEFVGQERVKEQLALLI
                     ESGRRRGEPIDHLLFSGPPGLGKTTLAGIVANELGAALRHTSGPALERTADLAAILTN
                     LEEGDVLFVDEVHRMPRAVEEVLYPALEDFKLDVVIGKGPSARTIRLELARFTMVAAT
                     TRPGLITLPLRDRFGFSPRLDYYGEADLRLVVGRSAGILGVEVDGAGATEIARRARGT
                     PRVANRLLRRVRDYVDVRGDGRIDGEGAVAALELFEVDDLGLDRIDVAILTTLVDRFR
                     GGPVGLATLAAAIGEDAGTLEEVYEPYLLQLGFLHRTPRGRVASELAYRHLGRPSDGR
                     LL"
     gene            complement(8897..>9301)
                     /gene="ruvA"
                     /locus_tag="VM638_02485"
     CDS             complement(8897..>9301)
                     /gene="ruvA"
                     /locus_tag="VM638_02485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HVM11331.1"
                     /translation="GMTLYGFSSPAERSLFLLLNGVTGIGPKVALAILSALSADALHR
                     AILEGDADAITVVPGVGKKVAARIVIDLRDKLGGEVEVPTAGPLSEVREALSALGMTP
                     QEVQRAVDGLEPDGQPVEEILREALRRVGAGA"
BASE COUNT         1421 a         3295 c         3250 g         1335 t
ORIGIN      
        1 gcggcgagac gtgcccctcc ccgatcccga cgagcatcga gtccaggccc gagaagttca
       61 gctccaccgc gacccgcgcg aggagctcct cggtcgtgag tcgcttgagc ggcagcccct
      121 ggcgccgcag ctcccgctgg aggagctccc gccccgcgtc catcgcgtcc tcgcgccgct
      181 cccgcgagaa ccactgccgg atcttcgtcc gggcgcgggg gctcttcacg atcgacagcc
      241 agtcgcgcgt cggaccggcc tcctgcgcct tcgacgtgat cacctcggcg gtgtcgccgg
      301 tctgcagctc gtagtcgagg gggacgagcc ggccgttcac cttcgcgccg atgcagcggt
      361 gcccgacctc ggtgtggatc gcgtacgcga agtcgatcgg cgtcgcgccc gcggggaggt
      421 tcatgacgtc ccccttcggg gtgaagacga agacctgatc ggcgtagagg tcgatcttca
      481 ggccttccat gaagtcgcgc gggtcggtgg cctccttctg ccagtcgagc atctgcgcga
      541 gccaggccat ctcggcctcg tccttcttct tcgccccctc cttgtagcgc cagtgcgccg
      601 cgatcccgta ctccgcggtg cggtgcatgt cccaggtccg gatctgcacc tcgaggggac
      661 gaccctcggg gccgatcacc gtcgtgtgca gcgactggta catgttgaac ttgggcatcg
      721 cgatgtagtc cttgaaccgg cccggcacgg gcttccagac ggagtggatc gcaccgaggg
      781 ccgcgtagca gtcgcgcacc tcgtcgacga ggacgcggat ccccacgagg tcgtagatct
      841 cggcgaactc cttgccgcgc agcaccatct tctcgtagac gctgtagagg tgcttcgggc
      901 gtccctcgat ctcgtgcttc accttcagct tcttcagcgt ctcccccacc gtctcgacga
      961 cctcacgcag gtacgcctca cgctcgtcct cggccttccc gacgaggttc gagatctcgg
     1021 tgaagcggcg gggatgcagg gtcgcgaaag cgaggtcctc gagctcccac ttgatctgga
     1081 agatcccgag ccggtgcgcg aggggcgcat agatctccag ggtctcggtg gccttctccg
     1141 tctgcttctc gcgggagagc gccccgaggg tgcgcatgtt gtggagccga tccgcgagct
     1201 tgatgatcag cacgcggatg tcccgtgcca tcgcgatgat catcttgcga acgttctcga
     1261 cctgctggtg ctccttcgtg cggaactcga tccggtcgag cttcgtcagc ccgtcgacga
     1321 tcagggcgac ctggtcgccg aactcctcgc ggatgtcctc gacggtcacc gcggtgtcct
     1381 cgacgacgtc gtggaggagc gcggcgatca cggtcgtcgc atccatcccg aggtcggcga
     1441 ggatctgcgc gaccccgagc gggtgggtga tgaaggcgtc gcctgacttg cggacctgcc
     1501 ctcgatgcga gcgctccgcg acgtcgtagg ccttgcggat caccggcagc tccgccttgg
     1561 cgttgtaggt gcggaccgtg cgcaggaggg cctcgagctg ggcgggcggc ttcggggatc
     1621 cggagcggaa gggcagacgg gcccagaggc cgccacgtcg gtccggtggc gcggcctccg
     1681 cgatgcgtgg gaccgagccc tgctccatca ccccattgta ggggcggtca gtacgtgagg
     1741 agggacgtga agtcgtagcc ctcgatcgcg cgacggccgt cgaggaagga aagctcgatc
     1801 acgcaggcga tgccgacgac cttgcccccg agacgctcga cgaggtcggc cgtcgcccgg
     1861 gccgtccctc cggtcgcgag gacgtcgtcg acgatcagta cccgctcgcc gggacggaag
     1921 gcgtccttgt ggatctcgag ggtctcggtc ccgtactcga ggtcgtactc ggccgcctcg
     1981 gtctcccacg ggagcttccc cttcttccgg accgggacga accccgcgcc gaagtggtag
     2041 gcgacggggg ccgccatgat gaacccgcga gcctcgatcc cgaccacctt gtcgacgttc
     2101 ccccgcccgt agtgcacgac gatcacatcg atcacggtcg agaacgcgac cgggtcggcg
     2161 agcagcggcg tgatgtcttt gaagacgatc ccgggcttcg ggaagtccgg gatgtcccgg
     2221 atgtggccgc ggaggcgctc gaggtcgttc acgcgcgcat cctactcgcc cctcaccgcc
     2281 gccgtcgccg tcgggcgtcg gagagccacc cccggcagga gacggacggc gaccggtccg
     2341 agcgcggcca tgaacaggac gtacgccgcg gccagcgaac cgagatccgg atgcagctga
     2401 gccgccaccg ccagaccggc gatcacgagg gagaactcac cgcgcgcgat cagcagcatc
     2461 cccgcccgca gacggccgcg gggcgcggcg ccctcgcgtc gtgcggcgac ccagcccgtc
     2521 cacaccttcg tcaccccgct gacgaaggcg aggacgagcg ccatgcccgc gacctgcggg
     2581 atcgacgatg gatcgacccg gaggccgaag aagacgaaga agaccgccgc gaacaggtcc
     2641 ctcagagggg tgaggacccc gcgggcgcgg tcggctgccc ggccggagag cgcgatgccg
     2701 acgaggaagg cgccgacggc cgccgagatg tggagccgct cggcgaggcc cgccacgaga
     2761 agggtgatcc cgacgatgct cagcagcagc gtctcgtccg agtggctgaa gacggctcgg
     2821 ctcagcgcct gaccgtgacg gagggcgagc gccaggatca ggatcgcgga gccgaccgcg
     2881 atgacgacgg tgaggctcag gcgcgcgaga ccgcccccct gcagcagcgc cgcggtgaac
     2941 gggaggtagg ccgccatgac cagatcctcg atcacgagga ccgacaggac cgcgggcgtc
     3001 tcccggttcc ccacccatcc caggtcgctc acctgcttcg cgacgatgcc cgacgaggag
     3061 atgtaggtga cgccgccgag gaagagggct gccagccacc cccagccgag cacgagggcg
     3121 aggaggaagc cgggcgagaa gttcagcacg aggtcgagcg cgccgaccgg cgccgagcgt
     3181 cgcaggctcg aggtcagctc ctccgccgag tactcgagcc cgagcatcag cagcagcagg
     3241 atcacgccga tctccgcccc gacctcgatg aagtcctcgg cggtcacgat cggcacgagg
     3301 cctccctctc ccacggcgag gcccgcgagc aggtacagcg ggatcggcga gaacccgaag
     3361 cggtgcgcga gccgggagac ggccgcgagc gcgaggatca cgagccccag ctcgaggagc
     3421 aggaccgctg acggcacgga tcagccggtc cgcagcagca ccgacaggtc ctcgatgccg
     3481 cgcggcgtcc cgacgacgac gagcgtgtcc cctgcctcca gcccgaagtc cgggccgggc
     3541 gccggatgcg cgtccgcgcc tcggacgacc gccacgatcg acgtcccggt cctggtgcgg
     3601 gcggcggtgt ctccgatcgt ccggccggcg tacggcgtgt cgtcgcggag cggcagccag
     3661 tcgatcgcga gcccctcgat ccgctggatc gtcgagagct gctcggcgac gcgggatccg
     3721 ccgaggacct cgatgagggt cctcgcgtcg tcgtcctcga gcgccaggac ctccgcgcac
     3781 atgtcgggat cgtcccggtc gtacagcaac agctcgcgcc ggccggtccg gtggtggagg
     3841 acccccaccc gcttgccggc ctcggtcagg aacgcgtagc ggatccccat ccccggaagg
     3901 accgtctctt cgatcttcgc catgcgcgca tcctcccacg cccgcggggc ggagcgaggc
     3961 tcagcgcttc ttgcgcttgg cccgaggctt cttcttcggc cgccgggggc gagccgcggg
     4021 cgtcgcgccg cgcgaccccg acccgctgcg gggggcgggc gcctcgacga cgtccgcctc
     4081 gacgacgtcg gcccctccgg ggacggcggc gagctccgga cgccgcgccc tggcccgctc
     4141 ccggagctgc gcgagccggg gctcccgctc cttgagcgtc gtcaggagcg gtgccgccac
     4201 gaagatcgag gagtaggcgc cgagcgcgac cccgacgaac agggcgaacg cgaagttctg
     4261 cagggtctca ccgccgaaga gcaggagggt gccgatcggc agcagcgtcg tgatgctcgt
     4321 gttgaccgat cgcatgaaca cctggttcag cgagaggttc acggcgcccg cgtacgtctc
     4381 cttcgccacg agcgagggcg acgccgcgtt gtcgcggatc ttgtcgaaga tcacgaccgt
     4441 gtcgtagagc gagtacccga ggatcgtcag gatcgcgatg acggtttcgg gggtgacctc
     4501 ccgtccggag agcgagtaga tcccggtcgt gatgatgaga tcgtggaaga gcgctgtgat
     4561 ggccgcgacg gacatcttcc actcgaaccg catcgcgatg tagatgctca cgacgacgag
     4621 gaagatgatc aggccctgga gcgccttccg ggagatctgc tccccccacc gcggcccgat
     4681 gacctgcgcg ttcacctggc cggcatcgat ccccgcctgc tcggcgaggg cggccgtgac
     4741 gtccgcccgg cggtcgccga ggtctccggt gcggacgctg acctcgctgt cgtcgacgac
     4801 ctgcacgacg gagtcgccgt atccgaagcg cgccatcgtc tcgcgaacct cggtggtcga
     4861 gacgccggcg gggttcggat agacgagctg ggtcccaccg cggaagtcga tgccgaggtt
     4921 gagacctcgg atgaacaggc tcccgatcga gagcacgacg agcagcccgg agatcgcgaa
     4981 ccacagccgc cgccggccga tgatgtccat gtggaactgg cggtgcccgc ggaaggtcgc
     5041 gatcgcggat cgcaggctca tcggctgcct ccccggaccg gcacgggatc gggatccgcg
     5101 gccaccccgg accgcagacc catcccccgc atctccgaga ggcgccggct gcgggcgatc
     5161 aggaagaccg tcggacgctt gaagaagaac atcacgatga ggtcgaggaa ggtggcgagg
     5221 ccgagcgtga gcgcgaagcc gcggacggcc ccgacggcaa cgaggtacag gatcaccgcc
     5281 gccgcgatcg tcaccaggtt cgcggcgacg atcgtgcgcc aggcggaggt gaaggcgggg
     5341 ccgaccgcgg ctcggagcgt cttcccgcgc cggacctcgt ccttcaggcg ctcgtagaag
     5401 acgacgtagg agtccgcggc gacaccgacc gagacgacca ggcccgcgac gccggcgagc
     5461 gtgaggctgt agccgacggt gcgtccgagc agcgacacga tcgcgagcgt gagcgtcgcc
     5521 cagatcgcca tcccgaacca cgtgacgacg ccgaggacgc ggtagtagaa gaacaggtag
     5581 atcgcgagcg cgatcagtcc ggccacgccg gcgaggagac cctggcgcag cgactcgcgt
     5641 ccgagggtcg ggctcaccgt gcggagctcc tcgcgctgca gctcgatcgg gagcgcgccg
     5701 gtgcgcagga cgagcgcgag gtccttcgcc tcctgctcct ggaagttccc cgagatctgc
     5761 ccctcgccgc ccgcgatctc ctcgttgatg ttcggggccg actcgacgcg ttcgtcgagg
     5821 acgatcgcga gctggcggtt cgtgttctcg cgcgtgatcc gcgcgaacgt ctccgcaccc
     5881 tcgccgttca ggcggaacga gacgacccag ccggagcttc cgggatcggt caccgccgcc
     5941 gggtcgttga agacggcccg ggcgtcgcgc agggcctcac ccgtcatctg gaccgggccg
     6001 aggcggtact tcgtccggtc ctcgccggac ccgagataga cgacctcgcc ctcggcttcc
     6061 tcctcctgct cctgctgctg ctcgtcggcc gtctcctcct cggccccggg cgagggggcc
     6121 ggcgtcggcg ccgcggaagg ctccgtggcc ccgtcctccc cctccgccgt cacctggagc
     6181 tcgtcgtact cgggcgtgcc gggcgccacg gcctcctcga cgatgcggaa ctccaggcgc
     6241 gcggtctgac cgatcagctc gagcagccgc tcctgtgatc cggtttcggc ctgcgggatc
     6301 tgcacgagga tgttgagctc accctgcctc gtgatgtccg gctcggcgac cccgaactgg
     6361 tcgacccggt tccggatcac ctcgagggtc ttgtcgacga cctcgctgtc ggtcccgggc
     6421 ggggcggtca acgtgagcga gacgccgccg acgaggtcga gcccgagctg cggccgcagg
     6481 ttcgccccgg agaaccccca gatcgccgca ccggcgatcg cgagcatgat cagggccgac
     6541 gcggcgagac gccccccgcc cctcacagga gcttcgcccc gttgatctcg aggtggaacc
     6601 cgcagtcgag ctcctccgcc gcgcggcggc acatgatcat gcgtccgagg aagatctcga
     6661 agtactccat gatgtcgctg atctgggtgt cgatcgtgag ctcgaggctg accgtcttcg
     6721 cggcggagtc tacacgcagg aacgagcgct ccgccgcgta gttcacgcgg tcgtggatgt
     6781 cgaactcgat ccggtcgtcc ttgcggaccc gcgaccggtg gacatcggcc ttgtccgcga
     6841 ggatcactgc cgcggcgacg ggcccggcgg tcatcccgtg ctcctcctcg tggttcccga
     6901 ccgcgcccat cacgatcgcg aggtcctgcg ggtccatgcg gtcggcgagg atctgctgca
     6961 cgaggatggc gcccgtctgg ccgtggacgg cgcgcgagac gaggttcccg atgtcgtgga
     7021 ggtagccgcc gatcgctgcg agcgtcacgg tccgctcgtc gtggccgagc cgctcgagga
     7081 ggttgcgcgc gatgctgccg gtgaggttcg cgtggcggaa cccgtgctcc gtgaagccca
     7141 ggccgtccat cacccggtcg gcctgcgcga tgaacgtcga gatgaccggg tcctgcttga
     7201 cgtcgtcgaa cgacagacct gcggaggcag ggtcacccac cggcgtcacg ctcccccgga
     7261 gccctccccc tcgagcggct cgggaacgag gcgccgggcg atcgcctgct tgacgaaccg
     7321 aacctcggtc cccgggctga tctcgagcga gacctcgtcg tcgctcatgc cacggatcct
     7381 gccgtacatc cctccgatcg tgacgacctc gtccccgacc tggagcgccg agacgaggtc
     7441 ccgctgctgc cgcacgcgcc gctgctgcgg gcggatcagc aggaagtaga agatcgccac
     7501 catcaggccg agcgagatga ggaacgtcgc gttcccgcgc ggctgctgcg tctgggcttg
     7561 ggctagcagt gtcgcgatca tgccgatgac ccccatgaaa aacggcggca cggctgccgc
     7621 cggtgagcgc atgatatcgc cgcacgggag cgggccgcca ggccgcgtca gcggcgcagg
     7681 aacaggttca cgaccagggt gaggacgatc gagaccagca gcatcgttcc gagcgggagg
     7741 tacacccgga cgttgccgcg cccgaacgtc aggtcgccgg gaagccgggg gatcaggccc
     7801 ttgcccaccg cgacgagcac gagccccacg agcgcgagca ccgcggccac gaccagcagc
     7861 acccgcccga acgcgtccat cgtcggagcc tagagcaggc ggccgtcgga ggggcggccg
     7921 aggtggcggt aggcgagctc gctcgccacg cggccgcgcg gggtgcggtg caggaacccg
     7981 agctggagca agtagggctc gtagacctct tcgagggtcc cggcgtcctc gccgatcgcg
     8041 gcggccagcg tggcgaggcc gaccggcccg ccgcggaagc gatcgacgag cgtcgtcagg
     8101 atcgcgacgt cgatccggtc gaggccgagg tcgtccacct cgaacagctc gagggccgcc
     8161 acggcccctt ccccgtcgat ccgaccgtct ccgcggacgt ccacgtagtc acggacacgc
     8221 cgcaggaggc ggttcgcgac gcgcggggtc ccgcgggcgc gtcgcgcgat ctcggtcgcg
     8281 ccggcgccgt ccacctcgac ccccaggatg cccgccgagc ggcccacgac caggcgcagg
     8341 tccgcctcgc cgtagtagtc gaggcgcggc gagaatccga agcggtcgcg caggggcagc
     8401 gtgatcaggc ccggacgggt cgtcgctgcg accatcgtga agcgcgcgag ctccagccgg
     8461 atcgtccggg cggacgggcc cttgccgatc acgacgtcga gcttgaagtc ctcgagcgcc
     8521 ggatagagga cctcctcgac cgcgcgcggc atccgatgga cctcgtcgac gaagagcacg
     8581 tcgccctcct cgaggttcgt gaggatggcg gcgaggtcgg ccgtgcgttc gagggccggc
     8641 cccgacgtgt gccgcagcgc cgccccgagc tcgttcgcga cgatcccggc cagggtcgtc
     8701 ttgcccaacc cgggcggacc ggagaacagg aggtggtcga tcggctcgcc ccgtcggcgt
     8761 cccgattcga tcagcagcgc gagctgctcc ttgacgcgct cctgaccgac gaactcctcg
     8821 agccgcttcg gccgcagggc gacgtccagc tcgcggtcga gctcgagctc gtcgggcgtg
     8881 acgatccgct cggcgctcat gctcccgccc cgacgcgccg gagcgcctcg cggaggatct
     8941 cttcgaccgg ctggccgtcc ggctcgagcc cgtcgaccgc ccgctggacc tcctgcggcg
     9001 tcatccccag cgccgacagc gcctctcgca cctccgacag ggggccggcg gtcggcacct
     9061 cgacctcgcc gccgagcttg tcgcggaggt cgatcacgat gcgcgccgcc accttcttcc
     9121 cgacccccgg aacgaccgtg atcgcatcgg cgtccccctc gaggatcgcg cgatgcagcg
     9181 catcggcgga gagggccgag aggatggcga gcgccacctt cgggccgatc ccggtgacgc
     9241 cgttcagaag gaggaacagg ctccgctcgg ccggcgagga gaacccgtag agggtcatcc
     9301 c
//
LOCUS       DATLGG010000088         5906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_38170, whole genome shotgun sequence.
ACCESSION   DATLGG010000088 DATLGG010000000
VERSION     DATLGG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38170"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1165
                     /locus_tag="VM638_02490"
     CDS             113..1165
                     /locus_tag="VM638_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM11332.1"
                     /translation="MDDRSTTPRASVEVHGLCKAFARHPVLRDVSLEVAPGTVLALLG
                     PSGCGKTTLLRTIAGLEVPDRGEIRVAGRVLAAAGVDVAPEDRRVGMVFQDWALFPHL
                     DVRRNVAFGLPRHEEHRVDELLALVRLDGLGERMPHELSGGQQQRVALARALAPNPEV
                     ILLDEPFSNLDATLRARVRSEVREMLRALHATAVFVTHDQEEALSIADEVAVMDAGRI
                     LQVGPPTEVYTDPAEPRVAEILGDGNLLPAEVRDGVATTVLGRIAAPGVAEGPAEVVV
                     RPEAIALAAEGVDAVVVDSEFYGHDQRARVKLHDGTLVDVRLLGEVEPLRPGARVRLS
                     VRGRTRFFPRPDRRSA"
     gene            complement(1162..2160)
                     /locus_tag="VM638_02495"
     CDS             complement(1162..2160)
                     /locus_tag="VM638_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HVM11333.1"
                     /translation="MAEAEVLRLAGREVKVTNPDKIYFPKRRITKLDLVRYYVAVAEG
                     ALVGCRNRPVILHRYPGGVEGEDFYQKRIPPQRPSWLQTMTITFPSGRSADELVVADA
                     AHLAWMVNLGCIDINPWPVRRDDPDHPDELRVDLDPTPRIPFASVRKVAMVVGEVLEE
                     HGLRGFPKTSGKRGIHINVRIEPRWGFTEVRRAALALAREVERRSSLATTAWWKEERR
                     GVFVDYNQNARDRTVASAYSVRPVPDARVSTPIAWDEVPEVEPRDFHLKSVPERFRTS
                     GDPGAGIDEAVGTLDALLELADRDEGGGLGDAPWPPHFPKGRTEPVRAAPSRRRRS"
     gene            complement(2162..2806)
                     /locus_tag="VM638_02500"
     CDS             complement(2162..2806)
                     /locus_tag="VM638_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234369.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HVM11334.1"
                     /translation="MSADRRRGVDGAAAYLPDELTLPAMRGAVDGCRGCDLWEGTTQG
                     VMGEGLKRAEVMFVGEQPGDREDVEGRPFVGPAGKELDRGLEEAGIDRRLAYVTNTVK
                     HFSFEQRGKRRIHRKPNAEQIRACRPWLEAELTVVRPRVLVALGATAAAALFGTKVRV
                     TRDRGKLVPSELAPVATLTIHPSAILRAPDEEIRRLERARFAEDLRLVAAHLGG"
     gene            complement(2825..3148)
                     /gene="fdxA"
                     /locus_tag="VM638_02505"
     CDS             complement(2825..3148)
                     /gene="fdxA"
                     /locus_tag="VM638_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010836864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HVM11335.1"
                     /translation="MTYVIAEPCIDVKDRACVDECPVDCIYEGGRMLYIQPDECVDCA
                     ACEPVCPVTAIFAEDDVPGEWKEYTAINAEFFTEEIAGYVSPGGAAQVGAVDRDHPAI
                     ANFSG"
     gene            complement(3229..3687)
                     /locus_tag="VM638_02510"
     CDS             complement(3229..3687)
                     /locus_tag="VM638_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11336.1"
                     /translation="MSTRFLPAPTIGWEPVYELLRCLGEATDDEVVRASECVSLITGM
                     QAGEPIDEWLLRELLALAQLDVDNHETLEAVGPAVGLLQGAGILRVEWESDSAFRRAA
                     NEDTDLFLGRRNSGAYASRVRSRMRRIEDGLRRALEAVEAFEDEERDLGD"
     gene            3788..4435
                     /locus_tag="VM638_02515"
     CDS             3788..4435
                     /locus_tag="VM638_02515"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVM11337.1"
                     /translation="MEPIRFTTADGLTLEGEVRRAESRARGTAVLCHPHPRHGGSKDH
                     PLLWTIRNDLAARRGATVVTFNSRGIMGSEGSFGSGIDERQDLAAAIDVARRLDGGPT
                     LVVGWSFGANLALCHAVSDPRIAGLALIGLPVAGIGPKVPDLPPREALRGLRAPVLLI
                     SGAADQFSPAPELRSLARKIGTAEVEVVPDSGHFFERRERWVAEQVGSFAERVLG"
     gene            complement(4432..4620)
                     /locus_tag="VM638_02520"
     CDS             complement(4432..4620)
                     /locus_tag="VM638_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11338.1"
                     /translation="MALIVIILLIWAAVSGVLWQVLRVAAGVALGIFFAGFLLIALVT
                     WAARRKLRRWTGGPFRRR"
     gene            4678..5466
                     /locus_tag="VM638_02525"
     CDS             4678..5466
                     /locus_tag="VM638_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVM11339.1"
                     /translation="MAIIIGTSGWQYDDWKGRFYPADLPKKGWLPFFSARFPTVEVNN
                     TFYNMPKAETFSTWQEVSAEGFIITIKASRYITHIRRLRECEDAVKLLWERCERLGEK
                     LGPVLFQLPPNLRADLELLASFLRVLPREMRAAFEFRHETWDTPEVRDTLHRHGSALV
                     LADRPGWKIRTEVTGGWSYVRFHQGTNLGPGYRRPRLRKWAEAIASLDAEETWVYFNN
                     DPTGAAIRDAVTLAELLLDLGADVLPGAAWVDRVPARAGRGTGP"
     gene            complement(5438..>5906)
                     /locus_tag="VM638_02530"
     CDS             complement(5438..>5906)
                     /locus_tag="VM638_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM11340.1"
                     /translation="GPVIDPGALERIEAWVDEAVAAGAEVLTGGRRRDPFYEPTVLAN
                     VNGDMKVCREEVFGPVTAVQRYETFEQALELANASRYGLQAGVFTSSIERAMLAHREL
                     VVGGVIVNDVSAYRADQMPYGGAKESGYGREGLRSAMEEMTEPRIMVLSHVPL"
BASE COUNT          822 a         2190 c         2034 g          860 t
ORIGIN      
        1 cagcgtacgc cgccacggct ctgctgcgcc gcctcgatgt cggtggcccg cagcctgtag
       61 cacctcacgg acacgcccgg cgccggcagc ggcggcgtac gctgagcgtg gcatggacga
      121 ccgttccacc accccccgtg cgagcgtcga ggtgcacggc ctatgcaagg ccttcgcccg
      181 ccacccggtg ctgcgcgacg tgtccctgga agttgcaccg ggcacggtgc tggccctgct
      241 cggacccagc gggtgtggca agacgaccct gctgcgcacc atcgccggcc tcgaggtccc
      301 ggaccggggc gagatccggg tggccgggcg ggtcctggcc gccgccgggg tcgacgtggc
      361 ccccgaggac cgccgggtgg gcatggtctt ccaggactgg gccctgttcc cccacctcga
      421 cgtgcgccgc aacgtcgcct tcgggcttcc ccgccacgag gagcaccggg tcgacgagct
      481 gctggccctc gttcgcctcg acggcctcgg cgagcggatg ccgcacgagc tctccggcgg
      541 gcagcagcag cgggtcgcgc tcgcccgtgc gctcgccccg aacccggagg tgatcctcct
      601 cgatgagccc ttctcgaacc tcgacgcgac cctccgcgcg cgcgtccgca gcgaggtccg
      661 cgagatgctg cgagcgctgc acgcgaccgc cgtcttcgtg acgcacgacc aggaggaggc
      721 gctctcgatc gccgacgagg tcgcggtgat ggatgccggc cggatcctcc aggtcgggcc
      781 ccccacggag gtgtacaccg acccggcgga accgcgggtc gccgagatcc tcggtgacgg
      841 taacctcctt cccgccgagg tccgggacgg tgtcgcgacg acggtgctcg ggcgcatcgc
      901 cgcgcccggc gtggccgaag gtccggccga ggtcgtcgtc cggcccgagg cgatcgccct
      961 cgccgccgag ggcgtcgacg cggtggtcgt ggactccgag ttctacggtc acgatcaacg
     1021 agcacgcgtg aagctgcacg acgggacgct cgtcgacgtc cgactgctgg gcgaggtcga
     1081 gccgctgcgt ccgggtgctc gcgttcggct ctcggtgcgg ggacggacga ggttcttccc
     1141 ccgcccggat cgccgctccg cctagctgcg gcggcggcgc gacggcgccg cccgtaccgg
     1201 ctccgtccgc cccttcggga agtgcggcgg ccatggggcg tccccgagcc ccccgccctc
     1261 gtcacggtcg gcgagctcga ggagcgcgtc gagggtcccc accgcctcgt cgatgcccgc
     1321 gccggggtca ccgctggtgc ggaagcgctc ggggacgctc ttcaggtgga agtcgcgcgg
     1381 ctcgacctcg ggcacctcgt cccacgcgat gggggttgag acccgagcgt ccggaaccgg
     1441 gcggaccgag taggcggagg cgacggtgcg gtcccgcgcg ttctggttgt agtcgacgaa
     1501 gacgccccgg cgctcctcct tccaccaggc cgtcgtcgcg agcgaggatc ggcgctcgac
     1561 ctcgcgcgcc aacgcgagcg ccgcccgccg gacctcggtg aagccccacc gcggctcgat
     1621 ccgcacgttg atgtggatcc ctcgcttgcc gctcgtcttc gggaagcctc ggaggccgtg
     1681 ctcctccagg acctcgccga cgaccatcgc caccttgcgg acgctcgcga acgggatccg
     1741 cggcgtcggg tcgaggtcca cgcgcagctc atccgggtga tcgggatcgt cgcggcggac
     1801 cggccacggg ttgatgtcga tgcagccgag gttgaccatc cacgcgagat gagccgcgtc
     1861 cgcgacgacg agctcgtccg ccgagcgtcc actcgggaag gtgatcgtca tcgtctggag
     1921 ccacgacggg cgctgcggcg ggatccgctt ctggtagaag tcctcaccct cgaccccgcc
     1981 ggggtagcgg tgcaggatca ccggccggtt gcggcacccg acgagggctc cctccgccac
     2041 ggcgacgtag tagcgcacca ggtcgagctt cgtgatcctg cgcttcggga agtagatctt
     2101 gtccgggttg gtgaccttga cctcgcggcc ggccagccgc aggacctcgg cttccgccac
     2161 gtcagcctcc gagatgggcg gcgacgaggc gcaggtcctc ggcgaatcgc gcacgctcga
     2221 gccggcggat ctcctcgtcg ggcgcccgga ggatcgccga cgggtggatc gtgagcgtcg
     2281 cgaccggggc cagctccgag ggcacgagct tcccgcggtc gcgcgtgacc cggaccttcg
     2341 tgccgaacag cgccgccgcc gccgtggccc ccagcgccac gaggacgcgc ggccgcacca
     2401 ccgtcaactc ggcttcgagc cacggccggc atgcgcggat ctgctcggcg ttcggtttgc
     2461 ggtggatccg ccgtttcccg cgctgctcga agctgaagtg cttcacggtg ttcgtgacgt
     2521 aggcgaggcg gcgatcgatc cccgcctcct cgagtccccg gtcgagctcc ttgccggccg
     2581 gcccgacgaa cgggcgcccc tcgacgtcct cgcggtcgcc gggctgctca ccgacgaaca
     2641 tcacctccgc ccgcttcagg ccctcgccca tcacgccctg ggtcgtcccc tcccacaggt
     2701 cgcagccgcg gcaaccgtcg accgctccgc gcatcgcggg aagcgtgagc tcgtcgggga
     2761 ggtacgccgc ggccccatcc acgccgcgac gccgatccgc cgacaccggc gctacgcgct
     2821 gacgctagcc gctgaagttc gcgatcgccg gatggtcgcg atccaccgcg ccgacctgcg
     2881 ccgcgccgcc gggggagacg taacccgcga tctcctcggt gaagaactcg gcgttgatcg
     2941 ccgtgtactc cttccactcc cccggcacgt cgtcctcggc gaagatagcg gtcaccgggc
     3001 agaccggctc gcaggcggca cagtccacgc actcgtcggg ctggatgtag agcatccgcc
     3061 cgccctcgta gatgcaatcc accgggcact catcgacgca ggctcggtcc ttgacgtcga
     3121 tgcagggttc cgcgatcacg tacgtcatgg gattttcccc ctatccaccg tggctaccgt
     3181 gcatatctgc cgtgattcta gcgcgggacg cgcgcccggg cgagggggtc agtcgccgag
     3241 gtcccgttcc tcatcctcga aggcctcgac ggcctcgagc gcccggcgga gcccgtcctc
     3301 gatccttcgc atcctggaac ggacgcggct cgcgtacgcg ccgctgttgc ggcggcccag
     3361 gaacaggtcc gtgtcctcgt tcgccgcgcg gcggaaggcc gagtccgact cccactcgac
     3421 gcggaggatc cccgcgccct gcaggagccc gaccgccggg ccgacggcct cgagcgtctc
     3481 gtggttgtcc acgtccagct gcgcgagggc gaggagctcc cggaggagcc actcgtcgat
     3541 cggctcgccc gcctgcatgc cggtgatcag cgagacgcac tccgacgccc gcacgacctc
     3601 gtcgtccgtc gcctcaccga ggcagcggag gagctcgtag accggctccc atccgatcgt
     3661 cggggcgggg aggaaccttg tgctcatgtc accactctct ccaccgaccg gcggacgcaa
     3721 acgcgcgatg gcctccgaag tgctcgcctt gccgggtgag cgacgcgtgt gcgatggtcc
     3781 cgcggcgatg gaaccgatcc ggttcacgac ggccgacgga ctcacgctgg aaggagaggt
     3841 gcgtcgcgcc gagagccgcg cccgggggac ggcggtgctc tgccatccgc acccgcggca
     3901 cggcggcagc aaggatcacc cgctgctctg gacgatccgc aacgacctcg ccgcacggcg
     3961 cggtgcaacc gtcgtcacct tcaactcccg cgggatcatg ggatccgagg gctcgttcgg
     4021 cagcggcatc gacgagcgac aggacctcgc cgcggcgatc gacgtagcgc ggcggctcga
     4081 cggcggaccg acgctcgtcg tcggctggtc cttcggcgcg aacctcgcgc tctgccacgc
     4141 ggtgagcgac ccgcgcatcg ccggtctcgc cctcatcggc ctccccgtcg cgggcatcgg
     4201 cccgaaggtg cccgatcttc cgccacgcga agcgctccga ggcctccgag cgccggtcct
     4261 gctgatctcc ggcgcggccg accagttctc accggctccc gagctgcgat ccctggcgcg
     4321 gaagatcggc accgccgagg tcgaggtcgt cccggattcc gggcacttct tcgagcgacg
     4381 ggagcgctgg gtcgcggagc aggtcgggtc gttcgccgaa cgggtgctgg gctagcgccg
     4441 gcggaagggc ccgccggtcc accgacgcag cttccgccgc gccgcccagg tcacgagcgc
     4501 gatcagcagg aacccggcga agaagatccc cagcgcgacc ccggccgcga cccgtaggac
     4561 ctgccagagg acacccgaga ccgcggccca gatcagcagg atgatcacga tcagcgccat
     4621 gcggccatca tcccccgccg aggcttcgag cgcacggcgc ggggggaaca tgaccgcgtg
     4681 gcgatcatca tcggaacgag cggctggcag tacgacgact ggaaggggcg cttctacccc
     4741 gctgacctcc cgaagaaggg atggctcccg ttcttctcgg cacgcttccc gaccgtggag
     4801 gtgaacaaca ccttctacaa catgccgaag gcggagacgt tctcgacctg gcaggaggtc
     4861 tccgccgagg gcttcatcat cacgatcaag gcgagccggt acatcacgca catccgccgg
     4921 ctccgcgagt gcgaggacgc cgtcaagctc ctgtgggagc gctgcgagcg tctcggggag
     4981 aagctcgggc cggtcctgtt ccagttgccg ccgaacctcc gagcggacct cgagctcctc
     5041 gcgtcgttcc tgagggtcct gccgagggag atgcgcgcgg ccttcgagtt ccggcacgag
     5101 acctgggaca cgccggaggt tcgggacacg ctccatcgcc acggatccgc gctcgtgctc
     5161 gcggaccgtc ctggctggaa gatccgcacg gaggtcaccg gcgggtggtc ctacgtgcga
     5221 ttccaccagg ggacgaacct cggccccggg taccgacgcc cgaggctccg gaagtgggcg
     5281 gaggcgatcg cctccctcga tgcggaggag acgtgggtct acttcaacaa cgacccgacg
     5341 ggggcggcga tccgcgacgc ggtgacgctg gccgagctcc tgctggacct cggcgctgac
     5401 gtgctccccg gcgccgcgtg ggtcgatcgc gtgcctgcta gagcgggacg tgggacagga
     5461 ccatgatccg cggctcggtc atctcctcca tcgccgagcg cagcccctcg cgtccgtagc
     5521 ccgactcctt cgcaccgccg tacggcatct ggtcggcgcg gtaggccgag acgtcgttca
     5581 cgatgacgcc gcccacgacg agctcgcggt gggcgagcat cgcccgctcg atgctcgacg
     5641 tgaacacgcc ggcctgcagg ccgtagcgtg aggcgttcgc gagctcgagc gcctgctcga
     5701 acgtctcgta gcgctgcacg gccgtgacgg gaccgaagac ctcctcgcgg cacaccttca
     5761 tgtccccgtt cacgttcgcg agcaccgtcg gctcgtagaa cgggtcccgt ctccttccgc
     5821 cggtgagcac ctcggcaccc gcggcgaccg cctcgtccac ccacgcctcg atccgctcga
     5881 gggcgccggg gtcgatcacc gggccg
//
LOCUS       DATLGG010000089         3124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_114384, whole genome shotgun sequence.
ACCESSION   DATLGG010000089 DATLGG010000000
VERSION     DATLGG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114384"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VM638_02535"
     CDS             <1..1112
                     /locus_tag="VM638_02535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HVM11341.1"
                     /translation="NSRASSSVFQPAFSASRLSAIRRARRATGSRWVTRTTGFEFPEI
                     DGLIESATLGETRGGDEEVPEVSAEAAVSRAGDLWKLGRHRLVCGDARDQACYARLLA
                     GERAAMGIHDAPYDLRIGGHVAKRGRHREFVMGSGELGDRFEPFLRDFLLASTGHLVP
                     GGFQFCFMDWRHMGEMLGAGRAAGLELKNLCVWNKGYGGMGSLFRSAHELVFVFRDPN
                     GPLRNNVQLGRFDRNRNNVWDYPGPQSLRKERELHPSPKPVAMIADAIRDVTDRSEIV
                     LDAFSGSGTTIIAAAKVGRRGYAIDLDPLYVDVAVRRWEAWSGETAVHAETGLTFAET
                     AELRAGSAESTIADDDPSTMPAPSFRVRQRARGAA"
     gene            1123..1563
                     /locus_tag="VM638_02540"
     CDS             1123..1563
                     /locus_tag="VM638_02540"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HVM11342.1"
                     /translation="MAKKKTGTTRQPYEIGRGKPPKSGQFPPGVSGNPGGRRKGSLNV
                     KAVLEAVMGTEITVPDRGGTRTVSILEALLLRQTQEALKGQLRAIESLLDRFERLGGD
                     PGPSHSEPNAEDLALLKEALEGLRSRRVPNRSAPAPEPDDDAGA"
     gene            1633..3039
                     /gene="terL"
                     /locus_tag="VM638_02545"
     CDS             1633..3039
                     /gene="terL"
                     /locus_tag="VM638_02545"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HVM11343.1"
                     /translation="MFATLEPGTAYQHNWHIDHLCWHLMRVARGEVRRLVITVPPRSM
                     KSITVSVGFTAWLMGHDPRQRIICVSYADALARKLSTDTRRVLEAPWFRELFPALRLA
                     SARPRQQELVTTEQGYRFAAGMSGAILGRGADLVVIDDPIKASDAFSEKERRRVNEAF
                     DNTLRTRLNDPRTGAIVIVMQRLHQDDLVGHVLEQEDWEVASVPAIETEERMYRVGDA
                     EEDVHLRRPGDVLDPVRLPREFLDRMRRAQGGLTFSAQYQQAPVPIDGNVIKREWLRF
                     YGERPAKFDLVVASWDTASTLSETADYSVGTVWGAKGLDFYLLDRVRGRFEVPELRRE
                     VVRIARQWKVNQTVIEGTDIGRAITQDLRRAGEYSAILRTPRFDKQARFLAQSARFES
                     GQVHAPENATWLAEWLNELLAFPNGRHDDQVDSTSQALDYLTARTHPLHAAQERRERP
                     QRVNRPARIVRPAGFARE"
BASE COUNT          497 a         1060 c         1072 g          495 t
ORIGIN      
        1 tgaattcgag ggcgagcagc tcggtgttcc agcccgcctt ctcggcgagc cggttgagcg
       61 cgatccggag ggcccgccgg gccacgggat cgaggtgggt gacccggacg accgggttcg
      121 agtttccgga gatcgacggc ctcatcgaga gcgccacgct tggcgagaca cgcgggggcg
      181 acgaggaagt gccggaggtt tcggccgaag cggcggtctc gcgggccgga gacctctgga
      241 agctcggtcg gcatcggctc gtctgcggcg atgcgcggga tcaggcctgc tacgcgcgcc
      301 tgctcgcggg cgagcgggcg gccatgggga tccacgatgc cccgtacgat ctgcgcatcg
      361 ggggtcatgt ggcgaagcgg ggccgtcatc gcgaattcgt catgggatcg ggcgagctgg
      421 gcgaccgctt cgagcccttc ctgcgggatt tcctgctcgc gagcaccggt catctggtgc
      481 cgggcggctt ccagttctgc ttcatggatt ggcgccatat gggcgagatg ctgggagcgg
      541 ggcgcgccgc cgggctcgag ctcaagaacc tctgtgtctg gaacaagggc tacggtggca
      601 tgggctcgct tttccggtcg gcccacgagc tcgtcttcgt gttccgcgac ccgaacgggc
      661 cgctgcgcaa caatgtccag ctcggccgct tcgaccgcaa tcggaacaac gtgtgggact
      721 atcccggtcc gcagagcctg cgcaaggagc gcgagctcca tccctcgccc aagcccgtcg
      781 ccatgatcgc ggatgcgatc cgcgacgtga ccgaccgcag cgagatcgtg ctcgacgcct
      841 tctccgggtc cgggaccacc atcatcgccg ccgcgaaggt tggccggcgc ggctatgcga
      901 tcgatctcga cccgctctat gtcgacgtgg cggtgcgccg gtgggaagcg tggtcgggcg
      961 agaccgccgt ccatgccgag accgggctca ccttcgccga aaccgcggag cttcgcgccg
     1021 gctcggccga atcgacgatc gcggacgacg atcccagcac gatgcccgct ccctccttcc
     1081 gggtgcggca gcgcgcccgc ggcgctgcgt gaggaggggg cgatggccaa gaagaagact
     1141 ggcaccacgc ggcagcccta cgagatcggc cggggcaagc cgcccaaatc ggggcagttc
     1201 ccgccgggcg tctccggcaa tcccggcggg cgccgaaagg ggagcctcaa cgtcaaggcc
     1261 gtcctcgagg cggtcatggg gaccgagatc acggtccccg atcgcggcgg cacgcggacc
     1321 gtctcgatcc tcgaagcgct cctcctgcgc caaacccagg aagctctgaa ggggcagctt
     1381 cgcgcgatcg agagcctcct cgaccgcttc gagcggctcg gcggcgatcc cggcccgagc
     1441 cattcggagc cgaacgcgga ggacctcgca ctcttgaagg aagccctcga ggggttgcgc
     1501 tcgcgtcgag ttcccaaccg ttcggctccg gcgccggagc cggacgacga cgcaggggct
     1561 tgagccatgg ccgaccgcgc gcgtctcctc cgggctctct tgaggcagga tcttgccacc
     1621 ttcaatcgga aggtgttcgc gaccctggag cccgggacgg cctaccagca caactggcat
     1681 atcgaccacc tgtgctggca cctgatgcgc gtggcgaggg gcgaggtccg acgcctcgtt
     1741 atcacggtgc cgccccgctc gatgaaatcg atcacggtct cggtcggctt cacggcctgg
     1801 ctgatggggc acgatccgag gcagcgcatc atctgcgtct cctatgccga cgcgcttgcg
     1861 cgcaagttgt ccaccgacac caggcgggtc ctcgaggccc cctggttccg cgagctcttc
     1921 cctgcccttc ggctcgcctc ggcccgcccc aggcaacagg agctcgtcac caccgaacag
     1981 gggtaccgtt tcgccgcggg catgagcggc gcgatcctcg gccgtggcgc cgacctcgtc
     2041 gtcatcgacg acccgatcaa ggcgtcggac gccttctcag agaaggagcg ccgtcgggtc
     2101 aacgaggcct tcgacaacac cttgcgcacc cggctcaacg acccccggac cggcgccatc
     2161 gtgatcgtga tgcagcgcct ccaccaggac gacctcgtcg gccatgtgct cgaacaggag
     2221 gattgggagg tcgcgtccgt gccggccatc gagacggagg agcgcatgta ccgcgtcggc
     2281 gatgccgagg aagatgtcca tcttcgccga ccgggcgacg tgctggatcc ggtgcggctc
     2341 ccgcgcgaat tcctggaccg gatgcgccgg gcgcaaggcg ggctcacctt ctcggcccag
     2401 taccagcagg cgcccgtccc gatcgacggg aacgtgatca agcgcgagtg gcttcgcttc
     2461 tacggcgagc gccctgcaaa attcgatctc gtggtcgcga gctgggatac ggcttccacc
     2521 ctgtcggaga cggcggatta ctccgtcggc accgtatggg gtgcgaaggg gctcgatttc
     2581 tacctgctcg atcgcgttcg gggccgcttc gaggtgccgg aactcaggcg cgaggtggtc
     2641 cgcatcgcgc ggcaatggaa ggtgaaccag accgtgatcg agggaaccga tatcggtcgc
     2701 gcgatcacgc aggatctgcg acgcgcgggc gagtacagcg ccattcttcg taccccccgc
     2761 ttcgacaagc aggcgcgctt cctggcccaa tcggctcgct tcgagtcggg tcaggtgcac
     2821 gcgcccgaga acgcaacgtg gctcgcggaa tggctgaacg aactcctggc tttcccgaac
     2881 ggccgccacg acgaccaggt cgattcgacg agccaagcgc tcgactacct caccgcgcgc
     2941 actcacccgc tgcacgcggc gcaggagcgg cgcgagcggc cccaacgcgt gaaccgcccc
     3001 gcgaggatcg tgcgtccggc ggggtttgcg cgcgagtagt cctcccgcac tgcttgggca
     3061 tcaaaacgcg agattggctg cagagtacgg tttggtttat caacggccct ccgtggcagc
     3121 gttg
//
LOCUS       DATLGG010000090         5406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_22461, whole genome shotgun sequence.
ACCESSION   DATLGG010000090 DATLGG010000000
VERSION     DATLGG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22461"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VM638_02550"
     CDS             complement(<1..753)
                     /locus_tag="VM638_02550"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVM11344.1"
                     /translation="MTRRVLLPCLVALLPLPASAAGTAGPTIHSVAGGIPEGVSPTTV
                     SVKPVGVDFDRQGNLYVADPGQGVVWRIDRENRIHHVAGTGRACYPDPSRCRAEGGSA
                     KEAFLQAPAGLAVSPDGSTVYVSEQRGHIVRAIDLANGKIRTVAGTGERGYSGAHEGA
                     PAAQATLYLPQSLDVDSRGRLYIADIGNKRIRRVETDGTIRTVAGCGSQVWPSCFYHA
                     RDGSPVEGTPIGANAGINGNGWGIAISPSDELY"
     gene            complement(936..1418)
                     /locus_tag="VM638_02555"
     CDS             complement(936..1418)
                     /locus_tag="VM638_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVM11345.1"
                     /translation="MTKTIEESIDVQVPVQTAYNQWTQFEEFPRFMEGVEEVRQMDDT
                     RLYWVAEVAGQRREWEAKITEQEPDQRIAWTAAGDVVHAGVVSFHRLDDATTRVMLQM
                     ELEPESWVDKAADALGITTSRIRGDLERFKEFIESRGQETGSWRGEVEPPGQVTQTDQ
                     "
     gene            complement(1490..2608)
                     /locus_tag="VM638_02560"
     CDS             complement(1490..2608)
                     /locus_tag="VM638_02560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVM11346.1"
                     /translation="MRRLARALPAATLLMATILQASPAPAQMLDPCRPVPIPGLCQPA
                     PLPTTPPAPDPDERDDPRERPKRQKRKGSEPGRPSPGRAASTASAGHSAHQAPFAISS
                     PNSTARLMDLLSSLVPEGTPLQDSLTQVVGPFPVAGPAWWIDDWHAPRSGGRVHLGLD
                     IFAPHGTPLVAAATGVVTQKGRGAMPGLYVEITDADGTQFFYCHLSRWAADVEIGQRV
                     EVGDVLGFVGNTGNAISTPPHVHFEIQPGGHPQPPKPWVDRWVRLAEQRALILIRRLG
                     GELPSDEAAAFRLTRLFDLAVEGSDPDALGHVLALAAMQPNVSSLEMAGSAAGQMAWE
                     IDWREPAEPAPPWGVPALPWQVSGVPRPDDHPPGDGSD"
     gene            2728..3597
                     /locus_tag="VM638_02565"
     CDS             2728..3597
                     /locus_tag="VM638_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVM11347.1"
                     /translation="METTTALLRWYGPRRTAFPWRGADPYGVLVSEVMLQQTQAARVA
                     RLYPDFVARFPSVDALAAASRAEVVRAWSGLGYNRRALSLSEAARAIVRDHAGRVPRD
                     PEVLQSLPGIGPYTAAAIAAFAYGREVPAIDTNVRRVVARARMGRDAHEVTSAEVRAE
                     AERWIRGVDPAAWNQALMDVGREVCRTKPACQRCPVARWCAFRARGAVPSRRPTGTSP
                     FEGSSRQVRGAVIHLLRPVGSLTLRGLAAGSGHPVEVVADAVRGLARDGLVRAGPAAL
                     RGAPSGRVRLSDA"
     gene            complement(3601..3756)
                     /locus_tag="VM638_02570"
     CDS             complement(3601..3756)
                     /locus_tag="VM638_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11348.1"
                     /translation="MPRCPLCDGGKVLVVIGASRRGLCLACGAEWVQEGSDQRRVQAA
                     AGEASPR"
     gene            3869..4495
                     /locus_tag="VM638_02575"
     CDS             3869..4495
                     /locus_tag="VM638_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HVM11349.1"
                     /translation="MALLLLIRHGMTDQTGKRLYGTSPGIRLSDEGRRQAEHLVHRLD
                     GLSLGALYSSPLERCRETIEPLARHLDLVPTDLDEVREVEYGELTGTAFEELRRLPLW
                     RRAEVAPSSVRFPGGETLGEVQQRAVAALAEVAERHRGSAVGVVTHGDVIRLALAHHA
                     GIHLDLYRRLEVDPASVSAVTIDRGGSRVVRVNDTGRMDDLADYTGSR"
     gene            4492..4983
                     /locus_tag="VM638_02580"
     CDS             4492..4983
                     /locus_tag="VM638_02580"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HVM11350.1"
                     /translation="MIEIDPVERIGSAPAPPPAEGDYVLQARGAGATMTLLVDRSQLE
                     LLSASIVSMLSGERLTGQDDALRAADVRDDHVDWRVGRILLGFDEDRRLFFLDAEELA
                     DDEADRPPASVRLWVEPRQMLALAEQASELLAQGLSRCQLCGNARDPAEHVCPALNGQ
                     SGH"
     gene            complement(4985..>5406)
                     /locus_tag="VM638_02585"
     CDS             complement(4985..>5406)
                     /locus_tag="VM638_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVM11351.1"
                     /translation="PDPPAAAGGVPVVRVVDGDTIRVRRGGREEPVRLIGIDTPEMGF
                     FGGVRECFAREASRFATGHLLGRRVRLELDVERRDRYDRLLAYVWLDGRLFNEMILRQ
                     GYASVATYPPNVRYVERFLRAERQARAEGRGLWSACT"
BASE COUNT          726 a         1920 c         1956 g          804 t
ORIGIN      
        1 gtagagctcg tcgctcgggc tgatcgcgat cccccagccg ttgccgttga tgcccgcgtt
       61 cgcgccgatc ggcgtcccct cgacgggcga gccgtccctc gcgtggtaga agcaggaggg
      121 ccagacctgc gagccgcagc ccgccaccgt gcggatcgtc ccgtcggtct cgacgcggcg
      181 gatccgcttg ttgccgatgt cggcgatgta gaggcggccg cgggagtcga cgtcgaggga
      241 ctgcggcagg tagagcgtcg cctgcgcggc gggagcaccc tcgtgagcgc cgctgtagcc
      301 gcgctcgcct gtacccgcga ccgtccggat cttcccgttc gcgaggtcga tcgcgcggac
      361 gatgtggcca cgctgctccg acacgtagac cgtggatcca tccggagaga ccgcgaggcc
      421 cgccggcgcc tggaggaagg cttccttggc cgaccccccc tcggcgcggc agcgcgacgg
      481 atcggggtag caggcgcgcc ccgtccccgc gacgtggtgg atccggttct cgcgatcgat
      541 gcgccagacg acgccctgac cggggtccgc gacgtagagg ttgccctgcc ggtcgaagtc
      601 gaccccgacg ggcttgaccg agaccgtcgt cggggagaca ccctcgggga tccctcccgc
      661 gaccgagtgg atcgtgggcc ctgccgtccc ggcggcggac gccggcagcg gaagcagtgc
      721 cacgaggcag ggcagcagaa cacgtcgcgt cacggcgagc tcctttcggt cgacgtgacg
      781 acgctggttc tacgctctca ggagatttcc tttgtgcagg cgcacaaaat cgggacgaaa
      841 cggcggcctc cgacgaagca gccgagcgcc cccacggccc gaacagcaaa gaggggcagg
      901 ttgccccgcc cctctttgcc gatcgttcgc aggccttact ggtcggtctg cgttacctga
      961 cccggcggct cgacctcgcc gcgccaggag cccgtctcct gaccgcgcga ctcgatgaac
     1021 tccttgaacc gctcgaggtc gccgcggatg cgcgacgtcg tgatgccgag cgcgtccgcc
     1081 gccttgtcga cccagctctc cggctcgagc tccatctgga gcatgacccg ggtggtggcg
     1141 tcgtcgaggc ggtggaagct gacgaccccg gcgtgaacca cgtcacccgc ggccgtccaa
     1201 gcgatccgct ggtccggctc ctgctcggtg atcttcgctt cccactcgcg ccgctgcccg
     1261 gccacctcgg cgacccagta gaggcgggtg tcatccatct ggcggacctc ctccacgccc
     1321 tccatgaagc gcgggaactc ctcgaactgg gtccactggt tgtacgcggt ctgtacgggc
     1381 acctgaacgt cgatgctctc ttcgatcgtc ttcgtcacgg ggcctcctcc tttcgacggt
     1441 tccttggtcg cttctacccg ccggagagcg agcgaaacga ccgaacccgt cagtcggagc
     1501 cgtccccggg gggatggtcg tcaggtcggg gcaccccgga cacctgccac gggagcgccg
     1561 ggacgcccca tggtggagcg ggctcagctg gctcccgcca gtcgatctcc caggccatct
     1621 gacctgccgc tgaccccgcc atctcgagcg aggagacgtt cggctgcatc gcggccagtg
     1681 cgaggacgtg gccgagcgcg tcagggtcgc taccttcgac ggcgaggtcg aacaggcggg
     1741 tgagtcggaa cgccgccgct tcgtctgagg ggagctcccc gccgagccga cggatgagga
     1801 tcagggctcg ctgttcggcg agccggaccc accgatccac ccagggtttg gggggctggg
     1861 gatggcctcc cggttggatc tcgaaatgga cgtgcggcgg ggtgctgatg gcgttcccgg
     1921 tgttgcccac gaacccgagc acgtctccga cctcgacccg ctgcccgatc tccacgtcgg
     1981 ctgcccaccg cgagaggtgg cagtagaaga actgcgtccc gtcggcgtcg gtgatctcca
     2041 cgtagaggcc cggcatcgcg ccgcgtccct tctgcgtgac gacgccggtc gccgcggcga
     2101 cgagcggggt cccgtgcggg gcgaagatgt ccaggccgag gtggacgcgg ccacccgaac
     2161 gaggcgcgtg ccagtcgtcg atccaccagg cgggcccggc gacggggaag ggtccgacga
     2221 cctgcgtgag cgagtcctga aggggggttc cctccgggac gaggctcgag agcaggtcca
     2281 tcagacgcgc ggtgctgttg gggcttgaga tcgcgaacgg cgcctgatgc gcgctgtggc
     2341 cggccgaggc ggtgctcgcc gcgcgaccgg ggctcggccg accgggctcg gatcctttcc
     2401 gtttctggcg cttcggccgc tcgcgtgggt cgtcgcgctc gtccgggtcg ggtgcgggcg
     2461 gggtggtcgg gaggggcgcc ggttggcaca ggccggggat ggggacgggc cggcacgggt
     2521 cgagcatctg cgcgggcgcc ggggaggcct gcaggatcgt cgccatcagg agcgtggccg
     2581 cgggcagcgc gcgtgcgagt cgtcgcatcc aacccatcat ctcgaaccgg accgatggga
     2641 tgcgatggcc catcgggagc atctcggtcc cacccgttcg ggccatgcag ctgtcgtgcg
     2701 gccggccgtc tcccgtaccc tcgcctcgtg gagacgacga cggccctcct ccgctggtac
     2761 ggaccccggc gcaccgcctt cccctggcgg ggggccgacc cgtacggggt cctcgtgagc
     2821 gaggtgatgc tccaacagac ccaggccgcg cgcgtcgcgc gcctctaccc ggacttcgtc
     2881 gccaggttcc catcggtcga tgcactggcg gcggcgtcgc gcgccgaggt ggtgcgggcc
     2941 tggagcggcc tcgggtacaa ccgccgcgcg ctctcgctct ccgaggcggc gcgagccatc
     3001 gtgcgcgacc acgcgggccg ggtccccagg gatccggagg tcctccagtc gctgcccggc
     3061 atcggcccgt acacggccgc ggcgatcgcg gcgttcgcgt acgggcgcga ggtcccggcg
     3121 atcgacacga acgtccgccg cgtggtcgct cgcgctcgga tgggccgcga cgcgcacgag
     3181 gtcacctcgg cggaggtccg tgccgaggcg gagcgatgga tccgcggggt ggatcccgcc
     3241 gcgtggaacc aggcgctcat ggacgtcggg cgcgaggtct gccgcacgaa gccggcgtgt
     3301 cagcgatgcc cggtagcgcg gtggtgcgcg ttccgagccc ggggtgcggt cccgtcgcgt
     3361 cgcccgaccg gaacttcgcc attcgaagga tcgtcccggc aggtccgggg cgccgtcatc
     3421 cacctcctgc ggccggtggg atcgctgacg ctccgagggc tcgccgccgg gtcggggcat
     3481 cccgtcgagg tcgtcgcaga tgcggttcgg ggcctggcgc gcgacggtct cgtccgggca
     3541 ggtcccgccg ccctgcgggg ggcgccctcg ggccgggtcc ggctcagcga cgcctagcgg
     3601 ctaccggggc gatgcctcgc cggccgcggc ctgaacgcgg cgctggtccg acccctcctg
     3661 cacccattcc gcgccgcagg cgaggcagag cccacgccgg ctcgcaccga tcaccaccag
     3721 caccttcccg ccgtcacaca gtgggcacct cggcatggct cctcctctgt ctccccctcg
     3781 aagggtcctc atcatgcccg cgggtcgagg cgaacagacg tccgtcacgt gacggttcga
     3841 tgacgaacga ccgatatgct tggcgccgat ggcgctcctg ctcctgatcc ggcacggcat
     3901 gaccgaccag accgggaagc gtctctacgg cacttctccg ggcatccggc tctcggacga
     3961 gggaaggcgg caggccgaac acctcgtcca ccgactcgac gggctctccc tcggcgcgct
     4021 gtactcgagc ccgctcgagc gctgccgcga gaccatcgag cccctggccc ggcacctcga
     4081 cctcgttccc acggacctgg atgaggtgcg ggaggtcgag tacggcgagc tgaccggcac
     4141 ggcgttcgag gagctccgtc gcctgccgct gtggcgccgt gccgaggttg cgccttcgag
     4201 cgtgcggttc cccggcggag agacgctggg agaggtgcag cagcgtgcgg tggcggcgct
     4261 cgcggaggtg gcggagcgac atcgggggag cgcggtgggc gtggtgactc acggtgacgt
     4321 gatccgcctc gcgctcgcgc atcatgccgg gatccacctg gacctgtacc gacggctcga
     4381 ggtcgatccg gcctccgtct cggccgtgac gatcgaccga ggcggatccc gcgtcgtgcg
     4441 ggtgaacgac actggccgga tggacgacct cgccgactac accgggtcac gatgatcgag
     4501 atcgatcccg tcgagcgcat cggcagcgcc ccggccccgc cgcccgccga aggcgactac
     4561 gtgcttcagg cgcggggagc gggagcgacg atgaccctcc tcgtcgatcg ctcccagctc
     4621 gagctcctgt cggcgtcgat cgtgagcatg ctttccggcg agcgcctcac ggggcaggac
     4681 gacgcgcttc gcgccgccga cgttcgggac gaccacgtgg actggcgcgt cggccggatc
     4741 ctgctcgggt tcgacgagga ccggcggctc ttcttcctcg acgccgagga gctcgcggac
     4801 gacgaagcgg atcggcctcc ggcatcggtt cgcctctggg tggagccgcg gcagatgctg
     4861 gcgctcgccg agcaggcatc cgagctgctc gcgcagggcc tgtcgcgctg tcagctctgc
     4921 gggaacgctc gggatcccgc cgagcacgtg tgtcccgcgc tgaacggcca gagcggtcac
     4981 tgagtcaggt gcacgcgctc cacaggccgc gcccctcggc cctcgcctgg cgctcggccc
     5041 gcaggaaccg ctcgacgtag cggacgttcg gcggataggt ggcgacggac gcgtacccct
     5101 gccggaggat catctcgttg aagaggcggc cgtcgagcca gacgtaggcg aggaggcggt
     5161 cgtagcgatc ccgacgttcc acgtcgagct cgagccgcac gcgtcggccg agcagatggc
     5221 ccgtcgcgaa gcggctcgcc tcgcgcgcga agcattcgcg gaccccgccg aagaagccca
     5281 tctccggcgt gtcgatcccg atcagccgca cgggctcttc ccgtccgccc cggcggacgc
     5341 ggatcgtgtc gccgtcgacg acccggacca cagggacgcc accggcggcg gcgggagggt
     5401 cgggag
//
LOCUS       DATLGG010000091         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_22555, whole genome shotgun sequence.
ACCESSION   DATLGG010000091 DATLGG010000000
VERSION     DATLGG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22555"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2432)
                     /locus_tag="VM638_02590"
     CDS             complement(<1..2432)
                     /locus_tag="VM638_02590"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HVM11352.1"
                     /translation="MTSRTRIAASLVVLAVIAPGGASAGGAWTKQTISNRNLAAVSFP
                     TPQRGWAVGTGGSVVATTNGGATWSAQTSGTSSDLRGVDFVDAARGWVVGLSDTVLST
                     SNGGATWKASTTGTGLNLVAVDFVDATHGWAVGYTASLVQMIVSTKDGGTTWTSQQAP
                     AGIGLLAISFADRLRGFTVGYLGAAYRTEDGGQTWLPMHVKADVGADLTSDSFYAVVR
                     MSADEGLVAGFRGQVARTEDGGLTWQVTGTLPNPVRSLAASGDAAFAVGDGGLVAEST
                     DAGKTWRLGASGTTLNLTGVSAPSPNRAFAVGAAGIGLAFVGEGAAATPLQMGLDPEI
                     CDGITKDWGTDAAGFVVPTITLDPTGNGDRAIVIGGTEGVRALRPFRPTAEATLWKLE
                     LQASFQRLAVAELDGDEGDELIAATAAPNAAARSGVTAVDGATGEALWSRRLPDGSRG
                     VRTSDLDGYGIDDVVAWSEDKAIYTLSGTNGSDLRAPKPVGERVRDLRLGHLNGDGVR
                     DIVAALHDGRVLALSGATGGPLWSHQVPRGELRGATLRDLSGDGIPEAIVAGAGESRS
                     VTPGNGDSVTTVGNIRGALVAVLDGRDGTLIWDYRQPGTVNFNAVTTADLNGDGVHDV
                     IGQVSRLDNSHVLALDGRGRSVNAVGTPEPLVLWTYSTARPGASPLQQASTPDALTVA
                     DGSGDGTPDVYAGLWSGGPFAVDGTPPAPDPDPAIRAPRAAGLWEVADRGIAYGVSVE
                     RFDGRLLILSSVSDGLVTLRDAGSGQVAWAYDAGIFPSIALGDLDGSPGRDVGVASVS
                     GRLSAVDPAGRLR"
     gene            complement(2513..>2936)
                     /locus_tag="VM638_02595"
     CDS             complement(2513..>2936)
                     /locus_tag="VM638_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11353.1"
                     /translation="HLAPEMGGMTAGTFRWRLVYAAPGGAQHQVVTTEAAGQVVAVDR
                     PSVGDYPAPVPPLAEMPSEVVSPAGAVAAWRAIAGPRAWLTGLRWQPLLDDRWYVMGG
                     VEARCGGTPCGTLFMQMPFDHDTGAAGTGEFPLPLPEP"
BASE COUNT          389 a         1111 c         1038 g          398 t
ORIGIN      
        1 cgcagccgtc cggcggggtc cacggcggag aggcggcccg agacactcgc gacgccgacg
       61 tcccgaccgg ggctcccgtc gaggtcgccg agcgcgatgc tcgggaagat cccggcgtcg
      121 taggcccagg cgacctggcc cgacccggcg tcccgcagcg tgacgagacc gtcggaaacg
      181 ctggagagga tcagcaggcg cccgtcgaag cgctcgaccg agacgccgta ggcgatccct
      241 cgatcggcga cctcccacag cccggccgcg cgcggcgcgc ggatcgcagg gtcggggtcg
      301 ggcgccggcg gggtgccatc gacggcgaac ggaccgccgc tccagagccc cgcgtagacg
      361 tccggtgttc cgtcgccgct gccgtcggcg accgtgagcg cgtcgggcgt cgaggcctgc
      421 tgcagcgggc tcgcgccggg ccgcgccgtc gagtacgtcc agagcacgag cggctcgggc
      481 gtcccgacgg cgttcacgct tcggccacgg ccgtcgaggg cgaggacgtg gctgttgtcc
      541 aggcgcgaca cctgtccgat cacgtcgtgg acgccgtccc cgttgaggtc ggcggtcgtg
      601 acggcgttga agttgaccgt tccgggctgg cggtagtccc agatcagcgt gccgtcccgc
      661 ccgtcgagga cggcgacgag cgcaccgcgg atgttcccca cggtggtgac gctgtcgccg
      721 ttgccggggg tcacggaccg gctctcaccc gcgcccgcga cgatcgcctc cgggatgccg
      781 tctccgctca gatcccgcag cgtcgccccg cgcagctcgc cgcgggggac ctggtgcgac
      841 cacagcggtc caccggtcgc gccggagagg gcgaggacgc gcccgtcgtg gagcgccgcc
      901 acgatgtcgc ggacgccatc accgttcagg tggccgaggc ggagatcgcg cacccgctcg
      961 cccaccggct tcggcgcgcg cagatccgag ccgttcgtcc ccgacagggt gtagatcgcc
     1021 ttgtcctccg accaggcaac gacgtcatcg atgccgtacc cgtcgaggtc gctggtccgg
     1081 accccccggc tgccgtccgg caaccggcgg ctccacaggg cctcacccgt cgcaccatcc
     1141 acggcggtca cgcccgacct cgcggccgcg ttcggcgcgg cggtcgcggc gatcagctcg
     1201 tctccctcgt cgccatcgag ctcggcgacg gccagtcgct ggaagctcgc ctgcagctcc
     1261 agcttccaga gcgtggcctc cgccgtgggg cggaacggtc gcagcgcgcg cacgccttcg
     1321 gtcccgccga tgacgatcgc tcggtcgccg ttcccggtcg ggtccagcgt gatggtgggg
     1381 acgacgaacc ccgcggcatc ggtcccccaa tccttcgtga tcccgtcgca gatctccggg
     1441 tccagcccca tctggagcgg cgtggccgcc gcgccctctc cgacgaaggc caggccgatc
     1501 cccgccgcgc ccaccgcgaa cgcccggttc ggcgagggcg ccgacacgcc ggtgaggttg
     1561 agcgtcgttc cggacgcgcc gagtcgccac gtcttcccgg catccgtgct ctccgcgacg
     1621 agacccccgt cgcccacggc gaacgccgcg tcgccgctgg cggcgaggct ccggaccgga
     1681 ttcggcagcg taccggtcac ctgccacgtg aggccgccgt cctcggtgcg cgcgacctgg
     1741 ccgcggaaac cggcgacgag accctcgtcg gccgacatgc gcaccaccgc gtagaacgag
     1801 tcggaggtca ggtcggctcc cacgtccgcc ttcacgtgca tcgggagcca ggtctggccg
     1861 ccgtcctcgg tgcggtacgc cgcacccagg tatccgaccg tgaatccccg gaggcgatcg
     1921 gcgaaggaga tcgccaggag gccgatcccc gccggcgcct gctgactggt ccacgtcgtt
     1981 ccgccgtcct tcgtcgagac gatcatctgc acgagcgagg cggtgtaccc gacggcccag
     2041 ccatgggtcg cgtcgacgaa gtcgacggcg accaggttca gcccggtgcc cgtcgtcgaa
     2101 gccttccacg tcgccccgcc gttcgacgtc gacaggaccg tgtcgctcag cccgaccacc
     2161 catccgcgag cggcgtcgac gaagtccacg ccgcgcaggt cactcgacgt tcctgacgtt
     2221 tgagcgctcc acgtcgcccc gccgttcgtc gtggcgacga cggaccctcc cgttcccacg
     2281 gcccacccac gttgcggcgt cgggaaggag acggcggcca ggttccggtt cgagatcgtc
     2341 tgcttcgtcc aggctccgcc ggcggacgcg ccgcccggcg cgatcacggc gaggacgacg
     2401 agcgacgcgg cgatgcgggt gcgagatgtc atgactcctc ttcgggtcgt ggtcgtcgtt
     2461 cgggtgttcc ttccaccccg ctccgacgga accctttgtg cacctgccta aatcagggct
     2521 cgggaagggg gagggggaac tcgccggtgc cggcggctcc ggtgtcgtgg tcgaacggca
     2581 tctgcatgaa cagcgtcccg cagggcgtcc cgccgcaacg cgcctcgacg ccgcccatca
     2641 cgtaccagcg gtcgtcgagg agcggctgcc agcgcaagcc ggtgagccag gcgcgtggac
     2701 cggcgatggc gcgccacgcg gcgaccgccc cggcggggga gacgacctcg gatggcatct
     2761 cggcgagcgg ggggaccggc gcggggtagt cgccgaccga tggccggtcc accgccacga
     2821 cctggcccgc ggcctccgtc gtcacgacct ggtgctgggc cccgccgggg gccgcgtaga
     2881 cgagccgcca gcggaaggtc cccgcggtca tcccacccat ctccggggcc agatgc
//
LOCUS       DATLGG010000092         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_53354, whole genome shotgun sequence.
ACCESSION   DATLGG010000092 DATLGG010000000
VERSION     DATLGG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53354"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM638_02600"
     CDS             <1..358
                     /locus_tag="VM638_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HVM11354.1"
                     /translation="QVAPVEPVVREEEALIRTIPQSRPPSGSAIHRAEPKRFNEARDL
                     GEKFKDGVPVIMNLQETEDTIARRLVDFASGLVFGLEGKIELVANRVYLLTPQEVEVS
                     AEERERIRDSGFYNQF"
     gene            417..689
                     /locus_tag="VM638_02605"
     CDS             417..689
                     /locus_tag="VM638_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVM11355.1"
                     /translation="MLIQIVCLVLFAFWLTLFARIILSWFPPPRSGAGAVVVEVIFDV
                     TEPVLRLVRGLLPPIRMGAMGLDLSPIIVFVALAVIRAALGCTVTI"
     gene            702..1481
                     /locus_tag="VM638_02610"
     CDS             702..1481
                     /locus_tag="VM638_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HVM11356.1"
                     /translation="MARKKKKDEAGFEQAPAGGRRRLTPVDVQEKVFRLAFRGYSEQE
                     VDEFLDQVTEDLAVLHEENKRLREKVEQGGAGSSVLPDAQRRADEIVRQAREQAARLL
                     ADAERRGGAGSGEGAAAAPASFLVRERDFLHQMASLIQGHAEALKQEARRAKEAEAAA
                     PPPPTAAAAAAGIAAAPAPVPPPMQEPPPAAAPAEPPAASSVDEPTQSHSPLLDEWEG
                     GVSGGPEDQPYEGSDLFGDPAPPAEGEDDQASLRELFWGEE"
     gene            1511..1765
                     /locus_tag="VM638_02615"
     CDS             1511..1765
                     /locus_tag="VM638_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11357.1"
                     /translation="MYAASLVFLLGAAASLYLGFRHGRLTFVFASIAASMLSMLFLGV
                     SILRRRSVEALAPRQPFPSAPPEEETPVAHDVPEDTPGTA"
     gene            complement(1766..>3534)
                     /locus_tag="VM638_02620"
     CDS             complement(1766..>3534)
                     /locus_tag="VM638_02620"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HVM11358.1"
                     /translation="RCREGHATVVGSLAELSDLAGRDVRGIDPHRPQIDEVEIRCPSC
                     GSEARRVPEVIDAWYDSGAMPFAQWDYHPDLGRGTDGFAARFPADFISEAVDQTRGWF
                     YSLMAEGVLLFDETAFRNCVVLGHVVDADGRKMSKRLGNVIDPFSVVERQGADAVRWF
                     MLTSGSPWQPRRVSMEILDEVVRRFLLTLWNVYSFFVTYANADGVDPTAEEPPGERPA
                     LDRWILSQLHRTIGEARDGLERYDATAAGRRIERFVDDLSNWYVRRARRRFWDPTQPD
                     GASPSKAAAYATLHTCLTTLARLLAPFTPFVADALWANLAAGRAGQPESVHLSDYPLA
                     DPDAVDEELDAAMQTVREVVELGRRVRNDARVKVRQPLPRAVLHLPGDPAPLAPLAPL
                     IADELNVKEVAFASSTDELAGWRAKPNYRVLGPRLGPRVKAVASALAGDDGSLAAALA
                     TGSTVSLEVDGEAVELGPEDVDLARETAGGWGIVSEGGLTVALDLEITPELRSEGLAR
                     EVVRVVQDARKAAGLDVADRIELSIESDGEVLQALRQHGDWVAAEVLAEKLRAEALPE
                     AEHRSEVELEAGRVTVALRRRG"
BASE COUNT          479 a         1274 c         1298 g          483 t
ORIGIN      
        1 gcaggtcgcg ccggtcgagc ccgtcgtgcg cgaggaggag gcgctgatcc gaacgatccc
       61 gcagtcacgg ccgccgtccg gctcggccat ccaccgtgcc gagccgaagc ggttcaacga
      121 ggcacgggac ctcggcgaga agttcaagga cggtgtcccg gtcatcatga acctccagga
      181 gaccgaagac acgatcgcgc ggcggctcgt ggacttcgcc tccgggctcg tcttcggtct
      241 cgaggggaag atcgagctcg tcgccaaccg cgtgtacctg ctcaccccgc aggaggtcga
      301 ggtctcggcg gaggagcggg agaggatccg ggactccggg ttctacaacc agttctagag
      361 gtggccgttg ccgacggggc tccggtagcc tcggcggctg tgccggagga gccgcgatgc
      421 tgatccagat cgtctgcctg gtgctgttcg cgttctggct gacgctgttc gcgcggatca
      481 tcctgtcgtg gttcccgcca ccgcggtcgg gggccggggc ggtcgtcgtc gaggtcatct
      541 tcgacgtcac cgagcccgtg ctcaggctcg tccggggact gctgccgccg attaggatgg
      601 gggcgatggg tctcgacctc tcgcccatca tcgtgttcgt ggccctcgcc gtgatccgcg
      661 cggcgctcgg gtgcacggtg acgatctagg gggttttccc gatggccagg aagaagaaga
      721 aggacgaagc cgggttcgaa caggcgccgg cgggtgggcg gcgtcgcctg accccggtcg
      781 acgtgcagga gaaggtgttc cgcctcgcct tccgcggcta cagcgagcag gaggtggacg
      841 agttcctcga ccaggtgacc gaggatctcg ccgtcctcca cgaggagaac aagcggctgc
      901 gcgagaaggt cgagcagggc ggcgccggct cgagcgtgct ccccgacgcc cagcgacgcg
      961 cggacgagat cgtgcggcag gcccgcgagc aggccgcgcg gctgctcgcg gacgctgagc
     1021 ggcgaggcgg ggccggctcc ggcgaggggg ccgcggccgc tcccgcgtcg ttcctcgtcc
     1081 gggagcggga cttcctgcat cagatggcct cattgatcca ggggcacgcc gaagcgctga
     1141 agcaggaggc gcgtcgcgcc aaggaggccg aggcggccgc gccaccgcca cccacggccg
     1201 cggccgctgc ggccgggatc gcggccgctc ccgcgccggt ccccccgccg atgcaggagc
     1261 cgccgccggc ggccgctccg gcggagcccc cggcggcatc gtcggtcgac gagccgacgc
     1321 agtcgcattc cccgctcctc gacgagtggg aaggtggcgt cagcggaggc ccggaggacc
     1381 agccctacga gggcagcgac ctgttcgggg accctgctcc gccggccgag ggtgaggacg
     1441 accaggcgtc gctgcgggag ctcttctggg gcgaggagtg agcgcccgcg ctcgcggctg
     1501 agggggtcgg atgtacgccg cgtccctcgt cttcctcctg ggggcagccg cctcgctcta
     1561 cctcggcttc cggcacgggc gtctgacctt cgtcttcgcc tcgatcgcgg cgagcatgct
     1621 ctcgatgctg ttcctcggcg tcagcatcct ccgtcgccgc tccgtcgagg cgctcgcccc
     1681 gcgacagccg ttcccgtcgg cgccgcccga ggaggagacg ccggtcgccc atgacgtccc
     1741 ggaggacacc cccgggacgg cgtgatcagc cgcggcgccg cagcgccacg gtgacccggc
     1801 cggcctcgag ctcgacctcg gagcggtgct cggcctcggg gagcgcttcc gcccggagct
     1861 tctccgcgag gacctccgcc gccacccagt cgccatgctg gcggagcgcc tggagcacct
     1921 caccgtcgct ctcgatcgag agctcgatcc ggtcggcgac gtcgagcccg gccgccttcc
     1981 gcgcgtcctg gacgacacgg acgacctcgc gtgccagccc ctcgctccgg agctccgggg
     2041 tgatctcgag gtcgagggcg acggtcagcc ccccctcgga gacgatcccc cagccacccg
     2101 cggtctcccg cgcaaggtcc acgtcctcgg ggccgagctc caccgcctcc ccgtcgacct
     2161 cgaggctcac ggtggatccg gtcgccaggg ccgccgcgag cgagccgtcg tcgccggcga
     2221 gcgccgaggc gacggctttc acccgcggcc cgaggcgcgg gcccaggacg cggtagttcg
     2281 gcttcgcccg ccagcccgcg agctcgtcgg tcgagctggc gaaggccacc tccttcacgt
     2341 tcagctcgtc ggcgatcagc ggggcgagtg gcgcgagcgg cgccgggtcg cccgggaggt
     2401 gcaggaccgc gcgggggagg ggctggcgga ccttcacgcg cgcgtcgttg cggacgcgcc
     2461 gccccagctc caccacctcg cgcaccgtct gcatcgccgc gtcgagctct tcgtccacgg
     2521 cgtcggggtc cgcgagcggg tagtccgaga ggtgcaccga ctcgggctgg ccggcgcgcc
     2581 ccgcggccag gttcgcccac agggcgtcgg cgacgaaggg cgtgaacggc gcgagcagac
     2641 gcgcgagggt cgtgaggcac gtgtgcaggg tcgcgtacgc cgccgccttc gacggcgacg
     2701 ccccgtccgg ctgggtcggg tcccagaagc gtcgccgggc ccgccggacg taccagttgg
     2761 agaggtcgtc gacgaagcgc tcgatacgcc ggcccgccgc ggtggcgtcg tagcgctcga
     2821 gaccgtcgcg agcctcgccg atcgtgcggt gcagctgcga gaggatccag cggtcgagcg
     2881 ccggacgctc cccgggaggc tcctccgcgg tcgggtcgac accgtcggcg ttcgcgtacg
     2941 tgacgaagaa cgagtacacg ttccacagcg tcagcaggaa gcggcgcacg acctcgtcga
     3001 ggatctccat cgagacgcgc cgcggctgcc acggggagcc gctggtgagc atgaaccacc
     3061 ggacggcatc ggcgccctgg cgctcgacca cgctgaacgg atcgatcacg ttgccgagcc
     3121 gcttcgacat cttccgcccg tccgcgtcga cgacgtgccc gagcacgacg cagttgcgga
     3181 acgcggtctc gtcgaacagg agcacgccct ccgccatcag cgagtagaac cagccgcgcg
     3241 tctggtctac cgcctcggag atgaagtcgg cggggaagcg cgcggcgaac ccgtcggtcc
     3301 cgcgcccgag gtcgggatgg tagtcccact gggcgaacgg catcgcgccc gagtcgtacc
     3361 aggcgtcgat cacctcgggg acgcgccgcg cctccgagcc gcaggatggg cagcggatct
     3421 ccacctcgtc gatctgcggc cggtgcgggt cgatcccccg cacgtcgcgc ccggcaaggt
     3481 cggagagctc ggcgagggac cccacgacgg tcgcgtgacc ctcgcggcag cgcc
//
LOCUS       DATLGG010000093         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_114530, whole genome shotgun sequence.
ACCESSION   DATLGG010000093 DATLGG010000000
VERSION     DATLGG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114530"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="glyS"
                     /locus_tag="VM638_02625"
     CDS             complement(<1..872)
                     /gene="glyS"
                     /locus_tag="VM638_02625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HVM11359.1"
                     /translation="MADLMDTIVAMAKRRGIVFQSSEIYGGLRSFWDYGPLGVELKRN
                     VRAAWWKAMVQERDDVVGLESSIMMSPRVWEASGHLDSFTDPLVECLSCHQRFREDHL
                     PESRACPNCGNNDWTDARNFNLMFKSFMGPVESAENEVYLRPETAQGMFVDFVTVQQA
                     SRKKLPFGIAQIGKSFRNEITPGNFIYRTREFEQMEMEFFVPPDEADEWHRYWIEERR
                     RWYVDLGLRESHLRVRAHEGDELSHYSSATSDIEYLFPIGWSELEGIAHRGSFDLTQH
                     TEASGTKLEWVDAGT"
     gene            1129..2592
                     /locus_tag="VM638_02630"
     CDS             1129..2592
                     /locus_tag="VM638_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HVM11360.1"
                     /translation="MSAPLRRLAVLVPLALLTHLAPAGAIDPVAPSLRLTAVETSDVT
                     LYNYGGHVPLDLGVYVASVGGDVRIDARAEEHGHAPVANQVDAESGTVLRPIPAEMLD
                     GWAGLKGFVRVTFTNTTGALVADQRFTFCPSGWDRQRVDDTGPPAQRYPSGCSEGFPF
                     LRGMVWGIEEGWATSLFGSEEEGPTGAVSVPRGRYTVRVEVDPGHATLFGIAEEDAAV
                     ELDVRVRRPPSSSWWSEDPLADGRLEPQGAATEITDPDPSTLPDLAALPAWSIGVRNN
                     ARTGRSWLRFAASPWNAGPAPLVVEGFRSQTQAKTMDAFQYFYDEDGSVVGRAPTGTL
                     VYHDAPGHHHWHFEMFALYTLLTQGPDGEEVQRSRKQGYCLVPTDAVDLTVPRAAFGP
                     SLTGLATACRGRSALSVREVLDAGWADTYYQSVAGQAINITKIPNGTYILRVTVNPGD
                     VLHEVTAANNVADRVIRLRGKKGARRVSVEPWNGVRA"
     gene            complement(2597..3055)
                     /locus_tag="VM638_02635"
     CDS             complement(2597..3055)
                     /locus_tag="VM638_02635"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HVM11361.1"
                     /translation="MRYFAYGVHMDPRAMAEACPGAPAGKVGRLDGYRLGFTVYSERW
                     EGGAANIEADPDAHVWGVVWEVPEECIRALDTYEGHPTFYRQEDVTVATGDRTVDCVT
                     YRVAHQTGFVRPSDAYVNVLRAAMRQQGLPPEALDILEAAARPPGPRIRS"
     gene            complement(3052..3783)
                     /gene="uppS"
                     /locus_tag="VM638_02640"
     CDS             complement(3052..3783)
                     /gene="uppS"
                     /locus_tag="VM638_02640"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HVM11362.1"
                     /translation="MTPSGIDPRRVPRHVAIVMDGNGRWARERGLPRTKGHEAGEEAL
                     FGIVEAALEVGVGFLTVYAFSTENWRRPASEVRFLMDFNRQLIRMRRDDLHERGVRLR
                     FLGRRERPLPKAVVREMDDAERLTRQNSRMQLIVALNYGGRAEIVDAVRALLADQRAG
                     RLRGRRVDARSLSRRMYHPDVPDPDLVIRTSGEKRLSNFLVWQAAYAELWFTPVLWPD
                     FSPQHLFDAIRDYQRRDRRFGGVGG"
     gene            complement(3780..4520)
                     /gene="recO"
                     /locus_tag="VM638_02645"
     CDS             complement(3780..4520)
                     /gene="recO"
                     /locus_tag="VM638_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HVM11363.1"
                     /translation="MPLYKDQGVVLGSIKLAEADKIVTVLTQGSGKVRAVAKGLRRTT
                     SRFGARLEPFTHVDLMLYRGRSLDTVTQAEIVHPFRTLREDYSLVAAGATMLEAADKV
                     AEEHERNVPLFLLLVKGLRALETRPADPAAVAESFLLRLLGLSGFAPSFTACAVCGST
                     EVGRFSHAQGGAVCETDRDRDAQRADPDALRWLATLATVTFEEAGAMTPPPRTRGAGR
                     ALLYAFTEWHLERRIRSLPGLVARRPPG"
     gene            complement(4562..4789)
                     /locus_tag="VM638_02650"
     CDS             complement(4562..4789)
                     /locus_tag="VM638_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVM11364.1"
                     /translation="MQTATEPSPPAASLTPRQVQIVRLMADGLRYAEIADELGLSAFT
                     VRNYVERIFERLGVYSRAGAVGRSLRDGLLD"
BASE COUNT          740 a         1766 c         1764 g          728 t
ORIGIN      
        1 gtcccggcgt cgacccactc gagcttcgtc ccggacgctt ctgtgtgctg cgtcaggtcg
       61 aagctgcccc tgtgggcaat cccttcgagc tccgaccacc cgatcgggaa gaggtactcg
      121 atgtcgctcg tcgcgctcga gtagtgcgag agctcgtcgc cctcgtgcgc gcgcacgcgc
      181 aggtggctct cgcgcaggcc gaggtcgacg taccagcgcc gccgctcctc gatccagtag
      241 cggtgccact cgtccgcctc gtccggcggc acgaagaact ccatctccat ctgctcgaac
      301 tcgcgcgtgc ggtagatgaa gttccccggg gtgatctcgt tgcggaatga cttgccgatc
      361 tgcgcgatgc cgaacggaag cttcttccgg ctcgcctgct gaacggtgac gaagtccacg
      421 aacatcccct gcgccgtctc cggacggagg tagacctcgt tctccgcgga ctcgacgggc
      481 cccatgaacg acttgaacat caggttgaag ttgcgcgcgt cggtccagtc gttgttgccg
      541 cagttcgggc aggcccggct ctccggcagg tggtcctcgc ggaagcgctg gtgacagctg
      601 aggcactcga cgagcgggtc cgtgaacgaa tccaggtggc cgctcgcctc ccagacgcgc
      661 ggggacatca tgatcgagga ctcgagcccg acgacgtcgt cgcgctcctg gaccatcgcc
      721 ttccaccagg cggcccggac gttgcgtttc agctccacgc cgagcggccc gtagtcccag
      781 aacgaccgca gcccgccgta gatctcggag gactggaaga cgatgccacg gcgcttcgcc
      841 atggcgacga tcgtgtccat caggtcagcc atccggctcc tctcccggcc gctggcggcg
      901 gccgctggcg tccggtcagg atatcccgcg ggggcactca gcgcggatcg aggcggtacc
      961 cgacgccccg aacggtgcgg atccgctcgc caccgagctt cttgcgcagg tacccgaccg
     1021 gggagctctc cgccgctgtc ccatacacgc ccgcggcctc gcgctgtggc acactggcgc
     1081 cacgcggcat ccgcgacggg acgccgcacg ggagggaagg agccggacat gtccgcacct
     1141 ctccgccgtc tggcggtcct cgttccgctc gcgcttctga cccatctcgc accggcggga
     1201 gcgatcgatc cggttgcgcc gtcgctccgg ctgacggccg tcgagacgag cgacgtcacg
     1261 ctgtacaact acggcggcca cgtgcccctc gacctcggcg tgtacgtcgc ctcggtcggg
     1321 ggcgacgtcc ggatcgacgc acgggcggag gagcatggtc atgcgcccgt ggcgaaccag
     1381 gtcgatgctg agagcggaac cgtcctgagg ccgatccccg ccgagatgct tgatggctgg
     1441 gccgggctca agggattcgt gcgcgtgacg ttcacgaaca cgaccggagc gctcgtggcg
     1501 gatcagcggt tcaccttctg cccgagcgga tgggaccgcc agcgcgtcga tgacacgggt
     1561 ccgccggcac agcgctaccc ctcgggttgc tcggaagggt tccccttcct ccgtgggatg
     1621 gtctggggca tcgaggaggg gtgggcgacc agcctgttcg gcagcgagga agagggcccg
     1681 accggagcgg tgtcggtccc ccgcgggcgc tacaccgtga gggtcgaggt cgaccccggc
     1741 catgcgacgc tgttcggtat cgctgaggaa gatgcggccg tcgagctcga cgtgcgcgtc
     1801 cggcgacctc cgagctcctc atggtggtcc gaggacccac tggcggacgg gcgtctcgag
     1861 ccgcagggag ccgccaccga gataaccgat ccggacccgt cgacgcttcc cgacctcgcc
     1921 gccctcccgg cgtggtcgat cggggtccgc aacaacgcgc gcacggggcg gtcgtggctg
     1981 cggttcgccg ccagcccgtg gaacgccgga cccgcgccac tcgtcgttga ggggttccgc
     2041 tcgcagacgc aggcgaagac gatggatgcc ttccagtact tctacgacga ggacggttcc
     2101 gtcgtggggc gcgcgccgac cggcacgctc gtctaccacg atgcgcccgg ccatcaccat
     2161 tggcacttcg agatgttcgc cctctacacc ctgctgacgc agggaccgga tggcgaggag
     2221 gtgcagcgga gcaggaagca ggggtactgc ctcgtcccga cggacgcggt cgacctgacg
     2281 gttccgcgtg ccgccttcgg tccgtcgctc accgggctcg cgaccgcctg ccgcggccgg
     2341 tcggcgctct ccgtgcgcga ggtcctcgac gccggctggg cggacacgta ctaccagagc
     2401 gtcgcggggc aggcgatcaa catcacgaag atcccgaacg gcacctacat cctgcgggtg
     2461 acggtgaacc cgggcgatgt cctgcacgag gtgaccgccg cgaacaacgt cgcggaccgc
     2521 gtcatccgcc tccgcgggaa gaagggcgcc cggcgcgtca gcgtcgagcc gtggaacggg
     2581 gtccgcgcct gaacgcctag cttcggatcc gggggcccgg cggacgagcg gccgcctcga
     2641 ggatgtcgag cgcctcgggc gggagcccct gctggcgcat cgccgcgcgc aggacgttca
     2701 cgtaggcgtc gctcggccgg acgaagccgg tctgatgcgc gacccgatag gtcacgcagt
     2761 cgacggtgcg gtcgccggtc gcgacggtga cgtcttcctg ccggtagaac gtgggatgcc
     2821 cctcgtacgt gtcgagggca cggatgcatt cctccggcac ctcccacacg accccccaga
     2881 cgtgcgcgtc gggatcggcc tcgatgttgg cggcgccccc ctcccagcgc tcggagtaga
     2941 cggtgaagcc gaggcggtac ccgtcgagcc gcccaacctt ccccgcgggc gcccccgggc
     3001 aggcttcagc catcgcccga gggtccatgt ggacgccgta ggcgaagtac ctcatccgcc
     3061 gacgcccccg aaccgacggt cgcgtcgctg gtagtcgcgg atcgcgtcga acaggtgctg
     3121 gggggagaag tccggccaca gcaccggcgt gaaccacagc tcggcgtagg cagcctgcca
     3181 gaccaggaag ttggagaggc gcttctcccc cgacgtcctg atcacgagat ccggatcggg
     3241 cacatcggga tggtacatcc ggcgggagag gctgcgcgcg tcgacccggc ggccgcgcag
     3301 ccgcccggca cgctggtcgg ccagcagtgc ccggaccgca tccacgatct cggcccgccc
     3361 gccgtagttc agcgcgacga tcagctgcat gcggctgttc tgccgggtca gccgttcggc
     3421 gtcatccatc tcgcgcacca ccgctttcgg gagtgggcgc tcacgccgcc cgaggaaacg
     3481 gagccggacg ccgcgctcgt gcagatcgtc ccgccgcatg cggatcagct gccggttgaa
     3541 atccatgagg aagcgcacct ccgaggccgg ccgtcgccag ttctccgtcg agaaggcgta
     3601 gaccgtgagg aagcccaccc ccacctcgag cgcggcctcg acgatgccga agagcgcctc
     3661 ctcccccgcc tcgtgcccct tcgtcctcgg gaggccgcgc tcgcgcgccc aacggccgtt
     3721 cccgtccatc acgatcgcga cgtggcgggg gacccgtcga ggatcgatcc cggagggcgt
     3781 catcccggcg gtcggcgggc gacgagaccg ggcaagctcc ggatccggcg ctcgaggtgc
     3841 cactcggtga acgcgtagag gagcgcccgt cccgcccccc gggtccgcgg tgggggtgtc
     3901 atcgcccccg cttcctcgaa ggtcacggtc gccagcgtcg ccagccagcg aagcgcgtcg
     3961 ggatccgcgc gctgggcgtc gcggtcccgg tccgtctcgc acacggctcc gccctgcgcg
     4021 tgggagaagc ggccgacctc cgtcgagccg cagaccgcac aggcggtgaa gctcggggcg
     4081 aacccggaga gcccgagcag gcggagcaag aacgattcgg ccacggcggc gggatcggcc
     4141 ggacgggtct cgagcgcgcg gaggcccttg accaggagca ggaagagcgg gacgttgcgc
     4201 tcgtgctctt ccgccacctt gtcggcggcc tcgagcatcg tcgcgccggc cgccaccagt
     4261 gagtagtcct cccgcagcgt ccggaaggga tggacgatct ccgcctgggt caccgtgtcg
     4321 agcgaccggc cgcggtagag catgaggtcg acgtgcgtga agggctcgag ccgggcgccg
     4381 aaccggctcg tcgtcctgcg gaggcccttc gccaccgccc ggaccttccc cgacccctgg
     4441 gtcaggacgg tgacgatctt gtccgcctcc gcgagcttga tcgagccgag aacgaccccc
     4501 tggtccttgt acagcggcat cgagcgaatc gtaccgccgg atgttgccgg gactgggagg
     4561 tctagtcgag gagaccgtcg cggagcgagc ggcccacggc gccggcgcgg ctgtagacgc
     4621 cgagccgctc gaagatgcgc tcgacgtagt tgcggacggt gaaggccgac agcccgagct
     4681 cgtcggcgat ctccgcgtac cggaggccgt cggccatgag gcgcacgatc tggacctgcc
     4741 ggggggtcag gctcgcggcg ggggggctcg gctcggtggc ggtctgcatg tcggcatcat
     4801 caccggatgt caccacccgg gggaatagcc ccgccggggc atcttcggcc cctccggccg
     4861 ggtggtgccg ggcgcggaga ggatgaggtc ggccggccta cgacggatgc agcgaagcga
     4921 gcagggcgtc gagcagcgac gacgccccga tgcggaagcg ctggggcgtg accgccgccg
     4981 gcccgagcac ggcgcgcg
//
LOCUS       DATLGG010000094         5850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_225042, whole genome shotgun sequence.
ACCESSION   DATLGG010000094 DATLGG010000000
VERSION     DATLGG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225042"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VM638_02655"
     CDS             complement(<1..573)
                     /locus_tag="VM638_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVM11365.1"
                     /translation="MKAVVLVGGGGTRLRPLTETTPKPLLPFMNRPLLHHVLDHLAEH
                     GVEEAILSSPYLEERFRGFLAERSGAPTLTWVTEPEPLGTFGAIAGAASHLDRTFLAL
                     NGDVVSDLDLTALVGFHRERQATATIALVPVADARPFGLVETEADGRILAFREKPEEP
                     KPGTINAGTYVLEPEVLEGAQRGRAISIERE"
     gene            complement(570..1427)
                     /locus_tag="VM638_02660"
     CDS             complement(570..1427)
                     /locus_tag="VM638_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVM11366.1"
                     /translation="MPAAPVGVVVVNHNSGEYLERCLSSVRAAAGEHPTEIVVVDNAS
                     TDGSIDGIEERHAGTRLIANGDNRGFAVAANQGIRATSTPYVLLLNPDAEIVGGTLEG
                     IVKVAEERPRAGVVGVLVRNSDGSLQPSARRVPGLGEALGHAFVGPFRPNNRWSRAYT
                     MADWDRTSEREVEWVSGSAMVIRREAFDEIGGFDEGFFMYVEDVDLCTRLRQRGWRVL
                     FSPELEVVHEQGVSSRARVRWLAFEHSRSIYRYFVKHRSPGAAALLRPFVRLALWGRA
                     LIASRGASR"
     gene            complement(1429..1896)
                     /locus_tag="VM638_02665"
     CDS             complement(1429..1896)
                     /locus_tag="VM638_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance protein"
                     /protein_id="HVM11367.1"
                     /translation="MTLGSPGRTAGAPVVVGDEAEGNGRRKLLVIAFILLSVALAAAA
                     QLTLKFGVDRVTEHGRSGIILGQPVASALRVAREPFVWAGLVLFGMSAAVWIVVLSRT
                     RLSFAYPFAALTYVIILLADRFLLKAQVPPLRWAGVALICTGIFLISRTGSAT"
     gene            complement(1893..2873)
                     /locus_tag="VM638_02670"
     CDS             complement(1893..2873)
                     /locus_tag="VM638_02670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM11368.1"
                     /translation="MSLLVVSSYPPRRCGIGAYAHTQVARMRGAGETVTVLSPPDGEG
                     DERIRFDGGRPFRRAVELTRPGDRILIHFQPALYFRPRAPLSKIGTSLGLLALVKRRP
                     RTEILVHEADPPRWWRPDEILLTRAFRAAPLLLFHTEAERRSFERRFGRIRRARVVPH
                     VDGVEVRGPVSRADARRRLELPAEARILVVPGFLHPGKGVDRAIRAVPQDALLYVVGS
                     VKDPTPENVAYAAEVRRLAAADPRVELVERFVADEEFDAWVAAADAVILPYRRSWSSG
                     VLARAQAIGTPAIVADVGGLGEQAGPSDVVVRSDAELAEAMAKVGHEVAS"
     gene            complement(2870..4054)
                     /locus_tag="VM638_02675"
     CDS             complement(2870..4054)
                     /locus_tag="VM638_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM11369.1"
                     /translation="MSAASVSIDAVSKRFRRYHERPGSLKERVLRMRGSRSEEFWALR
                     DVSYEIASGRTVALIGANGSGKTTLLKVIGGILRPTTGTVVTRGRVAALLELGAGFHP
                     ELTGRENVYLNASLLGLSRRETDRYFDEIVAFAELEEFIDNQVKFYSSGMYVRLGFAV
                     AVHVDPDILLVDEVLSVGDEAFQRKCMNRVKDFRQEGRTIIVVTHAVERVREICDEAV
                     MLERGRVAASGDVDRVVREFRLAMLRSHAGYQRADATREVEVTAVELLDPSGAPVGEV
                     VPGDPLVVQVETRVNRPVPDPVVWLSIHDATDQRVFSTSTELHGVSLDASDGAKQRIV
                     FRLGSIPFVEGRHFVSIGVRDRERTQVYDWHVQQYPFEIAETAAVSTRLHIPTDVRVE
                     PL"
     gene            complement(4051..4953)
                     /locus_tag="VM638_02680"
     CDS             complement(4051..4953)
                     /locus_tag="VM638_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM11370.1"
                     /translation="MAVAEERIRTSEPPRLRVRTRIADLWRRREVLVNLARKETKVKY
                     KSSALGVAWSMMNPLLYLVVFFVVFTFILPSRVPDFAVYLLSGLLAWALFSTGLSGAT
                     SSVVNNASLVGKVAFPREILPLSALGSSLVNFGFQFLVLLAVMLATGYGFLGSQIVLL
                     PLALAVLLLFTLAVGLLTSALNVRYRDTAHLVELLLLAWFWFTPVIYPARLVLDKSTE
                     LGSPLLFQLYQLNPMVHVVLGFQRALYGARTFPDGTTPVLPDAGLAWYAIRLGVLGAI
                     SLALLLLTWRLFFRRSGDFAEELG"
     gene            complement(4940..5782)
                     /locus_tag="VM638_02685"
     CDS             complement(4940..5782)
                     /locus_tag="VM638_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVM11371.1"
                     /translation="MRVLLTGSGGGLGTAFAAAAGAEHEVIAYPRTRLAVEDRLGVHE
                     AIAAARPDVVLNAAAMTSVDGCEVDPEAAYRANAFGARNVALAAAAAGALLIHVSTDY
                     VFDGRKGAAYHEFDEPNPISVYGHSKLGGEREVTAHAADHLIVRTSWVFGSGRDYLSG
                     ALGRLARGESAPAMGDLVGTPTHVRHLAERLIPLVDAGYRGLVHLGGPEATTWHDVLL
                     RAARDHSLPGEVVEQKAGDLDRPAARPPSSPLTSLVLRGDIPSMPPLAEAVREIVEGL
                     DGGR"
BASE COUNT          948 a         2101 c         2080 g          721 t
ORIGIN      
        1 ctcccgctcg atcgagatgg cccgcccgcg ctgcgccccc tcgagcacct cgggctccag
       61 gacgtaggtc ccggcgttga tcgtgccggg cttcggctcc tcgggcttct cgcggaaggc
      121 gaggatccgt ccgtccgcct cggtctccac gagcccgaac ggccgcgcgt cggcgacggg
      181 aacgagcgcg atcgtggccg tcgcctgacg ctcgcgatgg aacccgacga gggcggtcag
      241 gtcgaggtcg ctcacgacgt cgccgttcag cgcgaggaag gtgcggtcga gatgggaggc
      301 ggccccggcg atcgcgccga acgtcccgag gggctccggc tcggtgaccc aggtgagggt
      361 cggggccccg gagcgctccg cgaggaagcc gcggaagcgc tcctcgaggt acggggacga
      421 gaggatcgcc tcctccaccc cgtgctcggc caggtgatcg aggacgtggt gcaggagcgg
      481 acggttcatg aacggcaaga gcggcttcgg tgtcgtctcg gtgagcgggc gcaggcgcgt
      541 gccgcccccg ccgacgagca cgaccgcctt caccgcgagg ccccgcggga cgcgatgagg
      601 gcccgtcccc acagcgccag gcgcacgaac ggccggagga gcgcggcggc gcccggggac
      661 cggtgcttca cgaagtagcg gtagatgctc cgcgagtgct cgaacgccag ccaccggacc
      721 cgtgcgcgcg aggagacgcc ctgctcgtgg acgacctcga gctccggcga gaacaggacg
      781 cgccaccccc gctgccgcag gcgtgtgcag aggtcgacgt cctcgacgta catgaagaag
      841 ccctcgtcga acccgccgat ctcgtcgaac gcctcccgac ggatcaccat cgcggacccg
      901 gagacccact cgacctcacg ctccgaggtt cggtcccaat ccgccatcgt gtacgcgcga
      961 gaccatcggt tgttcgggcg gaaggggccg acgaaggcgt gcccgagcgc ttcgccgagt
     1021 cccggcaccc ggcgggcgga cggctgcagg gacccgtcac tgttgcgcac gagcaccccg
     1081 accaccccgg cgcgcgggcg ctcctcggcg accttcacga tcccctcgag cgtcccaccg
     1141 acgatctcgg cgtcggggtt caggagaagg acgtagggcg tggacgtcgc tcggatcccc
     1201 tggttcgccg cgaccgcgaa cccccggttg tcgccgttgg cgatgagtcg cgttccggca
     1261 tgccgttcct cgatcccgtc gatgctgccg tccgtcgacg cgttgtccac cacgacgatc
     1321 tcggtcggat gctcccccgc cgccgcgcgg accgaagaca ggcagcgctc gaggtactcg
     1381 ccggagttgt ggttcacgac gacgacgccg acgggggcgg ccggcatctc aggtcgccga
     1441 tccggtccgg gagatgagga agatcccggt gcagatcagc gcgacccccg cccagcgcag
     1501 cggcggcacc tgagccttga gcaggaagcg atcggccagc aggatgatca cgtacgtgag
     1561 cgcggcgaac gggtaggcga aggagagccg cgtccgcgac agcacgacga tccaaaccgc
     1621 cgcggacatc ccgaacagca cgagccccgc ccacacgaac ggctcgcgcg cgacgcgcag
     1681 tgccgaggcc accggctgcc ccaggatgat cccggagcgc ccgtgctcgg tgacgcggtc
     1741 gacaccgaac ttcagggtca gctgagcggc ggccgcgagc gccacggaca gcaggatgaa
     1801 cgcgatgacc aagagcttcc tcctcccgtt gccttccgcc tcgtcgccga cgacgaccgg
     1861 cgcgccggcg gtccgaccgg gtgacccgag cgtcacgatg ccacctcgtg cccgaccttc
     1921 gccatcgcct cggccagctc ggcgtcggac cgcacgacga cgtccgacgg ccccgcctgc
     1981 tcgccgaggc cgcccacgtc ggccacgatc gccggcgtcc cgatcgcctg cgcccgcgcc
     2041 agcacgccgg aggaccagga gcgacggtac ggcaggatga cggcgtcggc cgccgcgacc
     2101 cacgcgtcga actcctcgtc ggcgacgaag cgctcgacga gctcgacccg cgggtcggcc
     2161 gccgcgagcc gccggacctc cgcggcgtac gcgacgttct ccggtgtcgg gtccttgacc
     2221 gagccgacga cgtacaggag cgcgtcctgc gggacggccc ggatcgcccg gtcgaccccc
     2281 ttcccgggat gcaggaaccc cgggaccacg aggatcctcg cttccgccgg cagctccagg
     2341 cgccgccgcg cgtccgcacg ggagacgggg ccgcggacct cgacgccgtc gacgtgtggg
     2401 acgacgcgcg cgcggcggat ccggccgaac cgacgctcga acgaccgtcg ctcggcctcg
     2461 gtgtggaaca gcagcagcgg cgccgcgcgg aacgctcgcg tgagcaggat ctcgtcgggc
     2521 cgccaccacc gcggcgggtc ggcctcatgg acgaggatct ccgtgcgcgg ccggcgcttg
     2581 acgagcgcga ggaggccgag cgaggtcccg atcttcgaga gcggggcgcg gggacggaag
     2641 tagagggccg gctggaagtg aatgaggatc cggtcgcccg gccgcgtcag ctccacggcc
     2701 cgccggaacg gacgtccgcc gtcgaaccgg atgcgctcgt ccccttcgcc atccggggga
     2761 gacaggacgg tcacggtctc gccggcgcca cgcatccggg cgacctgcgt gtgcgcgtac
     2821 gcgccgatgc cgcagcggcg cggggggtac gacgacacga cgaggagact cacagcggct
     2881 ccacccgcac gtcggtcggg atgtgcaggc gcgtggagac ggcggcggtc tcagcgatct
     2941 cgaacgggta ctgctggacg tgccagtcgt agacctgcgt gcgttcgcgg tcgcgaacgc
     3001 cgatcgagac gaagtgacgt ccctcgacga aggggatcga cccgaggcgg aacacgatcc
     3061 gctgcttcgc tccatcggac gcatccaagg acacgccgtg gagctcggtc gacgtcgaga
     3121 agacccgctg atcggtcgcg tcgtggatcg agagccacac gacgggatcg gggaccgggc
     3181 ggttgacgcg ggtctcgacc tgcacgacga gcgggtcgcc cggcaccacc tccccgaccg
     3241 gggcgccgga ggggtcgagc agctcaacgg ccgtcacctc gacctcgcgc gtcgcgtcgg
     3301 cccgctggta tccagcgtgc gagcggagca tcgcgagacg gaactcccgc acgacgcgat
     3361 cgacgtcgcc ggacgccgcg acgcgaccgc gctcgagcat caccgcctcg tcgcagatct
     3421 cgcgcacccg ttcgacggcg tgggtcacga cgatgatcgt ccggccctcc tgccggaagt
     3481 ccttcacccg gttcatgcac ttgcgctgga acgcctcgtc gcccaccgac agaacctcgt
     3541 cgacgagcag gatgtcggga tccacgtgaa cggccacggc gaaaccgagc cgcacgtaca
     3601 tgcccgagga gtagaacttc acctggttgt cgatgaactc ctcgagctcg gcgaaggcca
     3661 cgatctcgtc gaagtaccga tcggtctccc gcctgctgag cccgagcaac gaggcgttca
     3721 ggtagacgtt ctcgcggccc gtgagctcgg ggtggaaccc cgcgccgagc tcgagcagcg
     3781 ccgcgacgcg accgcgggtg acgaccgtcc ccgtggtcgg acgcaggatc ccgccgatca
     3841 ccttgagcag cgtcgtcttg cccgagccgt tcgcgccgat caacgcgacg gtcctcccgg
     3901 atgcgatctc gtacgacacg tcgcgcaggg cccagaactc ctcggaacgg ctgccccgca
     3961 tccgcaggac ccgctccttc aagctgcccg ggcgctcgtg gtagcggcgg aagcgcttcg
     4021 agaccgcgtc gatcgagacc gaggccgcgc tcatccgagc tcctcggcga aatctcccga
     4081 gcggcggaag aagaggcgcc acgtcagcag cagcagcgcg agcgagatcg cgccgaggac
     4141 gccgaggcgg atcgcgtacc aggccaggcc cgcgtccggc agcaccgggg tcgtcccgtc
     4201 cgggaacgtc ctcgccccgt agagggctcg ttggaagccg agcacgacgt ggaccatcgg
     4261 gttgagctgg tagagctgga agagcagggg cgagcccagc tccgtgctct tgtcgaggac
     4321 cagtcgggcc ggatagatga ccggcgtgaa ccagaaccag gccagcagca gcagctccac
     4381 gaggtgcgcc gtgtcgcggt agcggacgtt cagcgcggag gtgagcaggc cgaccgccag
     4441 ggtgaacagc aggaggaccg cgagcgcgag gggaagcagc acgatctgcg agccgaggaa
     4501 cccgtagccg gtcgcgagca tgacggcgag caggacgagg aactggaagc cgaagttgac
     4561 gagcgacgat ccgagggccg acagcggcaa gatctcccgg gggaacgcga ccttccccac
     4621 gagcgaggcg ttgttcacga ccgacgacgt cgcgccgctc aaccccgtcg agaagagcgc
     4681 ccacgcgagc aaccccgaca gcaggtagac cgcgaagtcc ggcacgcggc tcggaaggat
     4741 gaaggtgaac acgacgaaga acacgacgag gtagagcagc gggttcatca tcgaccacgc
     4801 gacaccgagc gccgaggact tgtacttcac cttcgtctct ttcctcgcga ggttcacgag
     4861 cacctcgcga cggcgccaga gatcggcgat ccgcgtgcgg acgcggagcc gaggcggctc
     4921 gctcgtgcgg atccgctctt cagcgaccgc catcgagacc ctccacgatc tcgcgcacgg
     4981 cctcggccag cggcggcatc gaggggatgt caccccgcag gacgaggctc gtcagcggtg
     5041 agctcggcgg ccgcgcggcg ggacgatcca ggtcgcccgc cttctgttcg acgacctcgc
     5101 cgggcaggga atggtcccgg gcggcgcgca ggaggacgtc gtgccaggtg gtcgcctccg
     5161 ggccgccgag atggacgagg ccccggtacc cggcgtcgac gagcgggatc agccgctccg
     5221 cgaggtggcg gacgtgcgtc ggcgtcccga cgagatcgcc catcgccggt gcgctctccc
     5281 cccgcgcgag ccgtccgagc gcccccgaca ggtagtcacg gccgctgccg aagacccacg
     5341 acgtccggac gatgaggtga tccgccgcgt gcgccgtgac ctcgcgctcg ccccccagct
     5401 tgctgtggcc gtagacggag atcgggttcg gctcgtcgaa ctcgtggtag gccgccccct
     5461 tccgcccgtc gaacacgtag tccgtcgaca cgtggatcag gagcgcgccc gccgccgcgg
     5521 cggcgagcgc cacgttgcgt gcgccgaagg cgttcgcgcg gtacgccgcc tcggggtcga
     5581 cctcgcagcc gtcgacggac gtcatcgcgg cggcgttcag gacgacgtcg ggtcgagcgg
     5641 cggcgatcgc ctcgtgaacg ccgaggcggt cctccacggc cagacgcgtg cggggatagg
     5701 cgatcacctc gtgctcggcg cccgcggcgg cggcgaacgc ggtgccgagc ccgccacccg
     5761 aaccggtgag gaggaccctc acaggacgcc cccccgcccg acgacgatcg cgacgtcggc
     5821 gttgccgacg accgcgcggc cggcgcgccg
//
LOCUS       DATLGG010000095         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_114700, whole genome shotgun sequence.
ACCESSION   DATLGG010000095 DATLGG010000000
VERSION     DATLGG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114700"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VM638_02690"
     CDS             <1..2260
                     /locus_tag="VM638_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HVM11372.1"
                     /translation="EALSLGHNYIGTEHILLGLVRENEGVAARILLDFDADAEKIRNE
                     IIRMLSGPGRRQGGAAGAAGEKSKSSKLLDQFGRNLTKAASEGKLDPVVGRQTEIERV
                     MQILSRRQKNNPVLIGEPGVGKTAVVEGLANRISTNQVPEILKGKQIYTLDLAALVAG
                     SKYRGEFEERLKKVMKEIMQRGDIVLFIDELHNLVGAGAAEGAIDAASILKPALARGE
                     LQTIGATTLDEYRKYLERDAALERRFQQVRVDEPSIDDTVQILRGLRDRYEAHHRCQI
                     SDDALRAAAVLADRYIQDRHLPDKAIDLIDEAGSRLRIKTMSAPPRYRELEDEIERVR
                     TEKEAAIEAQEFEKAASLRDKERKLTQKKKELEENWRAEESEEQPVVGEDEIADIVSM
                     WTGIPVFKLTEAESQKLIRMEDELHKRVIGQHQAVVAVAKAIRRARAGIKDPKRPTGS
                     FIFLGPSGVGKTELARTLAEFLFGDEDAMIQIDMSEYMEKHSVSRLVGSPPGYVGYDE
                     GGQLTEAVRRKPYSVLLLDEIEKAHPDVFNILLQILEEGKLTDSQGRKVDFRNTIVIM
                     TSNIGAGQIAKNQTLGFSFGDEVGLSYDEMKGRVMGDLKKVFRPELLNRIDETIVFHK
                     LGKEEIFTIVDLQLKRLREQMAAYEVAIELTDAAKDVLVEQGFDPAMGARPLRRAIQR
                     FIEDPLADFVLGRELNPGATILVDRKEELTGEENESLVEIRIVEGEASGEKVTVPAAE
                     PAEPDEAPADEE"
     gene            2378..>2516
                     /locus_tag="VM638_02695"
     CDS             2378..>2516
                     /locus_tag="VM638_02695"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11373.1"
                     /translation="MAKAAVNYACTECGYSAGRWFGKCPGCNAFGSLVEELPPDAARP
                     QA"
BASE COUNT          456 a          852 c          880 g          328 t
ORIGIN      
        1 ggaggcgctc tccctcggcc acaactacat cggcacggag cacatcctgc tcggcctcgt
       61 ccgcgagaac gagggcgtcg ccgcgcggat cctgctcgac ttcgacgccg acgcggagaa
      121 gatccgcaac gagatcatcc ggatgctctc cggccccggc cgccgccagg gcggcgccgc
      181 cggcgccgcg ggcgagaagt cgaagagctc gaagctcctc gaccagttcg gccgcaacct
      241 gacgaaggcc gcctccgaag ggaagctcga ccccgtcgtc gggcgccaga ccgagatcga
      301 gcgcgtgatg cagatcctct cgcgccggca gaagaacaac ccggtgctga tcggcgagcc
      361 cggcgtcggc aagaccgccg tcgtcgaggg cctcgcgaac cggatctcga ccaaccaggt
      421 gcccgagatc ctgaagggca agcagatcta cacgctcgac ctcgccgccc tcgtcgccgg
      481 ctcgaagtac cgcggcgagt tcgaggagcg gctgaagaag gtcatgaagg agatcatgca
      541 gcggggcgac atcgtcctct tcatcgacga gctccacaac ctcgtcgggg cgggtgccgc
      601 cgagggcgcg atcgacgcgg cctcgatcct gaagccggcg ctcgcgcgcg gtgagctgca
      661 gacgatcggc gcgacgacgc tcgacgagta ccgcaagtac ctggagcgcg acgcggcgct
      721 cgaacggcgc ttccagcagg tgcgcgtcga cgagccgtcg atcgacgaca cggtgcagat
      781 cctgcgcggc ctccgcgacc gctacgaggc gcaccaccgc tgccagatct cggacgacgc
      841 actccgcgcc gccgccgtgc tggccgaccg gtacatccag gaccggcacc tcccggacaa
      901 ggcgatcgac ctcatcgacg aggcaggctc gcgcctgcgc atcaagacga tgagcgcgcc
      961 gccgcggtat cgcgagctcg aggacgagat cgagcgcgtg cgcaccgaga aggaggccgc
     1021 gatcgaggcg caggagttcg agaaggccgc ctcattgcgc gacaaggagc gcaagctgac
     1081 gcagaagaag aaggagctcg aggagaactg gcgcgccgag gagtccgagg agcagccggt
     1141 cgtgggcgag gacgagatcg ccgacatcgt ctcgatgtgg acgggcatcc cggtcttcaa
     1201 gctcaccgag gcggagtcgc agaagctgat ccgcatggag gacgagctgc acaagcgcgt
     1261 gatcggccag caccaggcgg tcgtcgcggt ggcgaaggcg atccgccgcg cccgcgcggg
     1321 gatcaaggac ccgaagcggc cgaccggctc gttcatcttc ctcggcccgt cgggcgtcgg
     1381 caagacggag ctcgcgcgca cgctcgccga gttcctcttc ggcgacgagg acgcgatgat
     1441 ccagatcgac atgtcggagt acatggagaa gcactccgtg tcccggctcg tcggctcccc
     1501 gcccggctac gtcggctacg acgagggcgg ccagctcacc gaggccgtgc ggcgcaagcc
     1561 gtactcggtg ctcctgctcg acgagatcga gaaggcgcac cctgacgtct tcaacatcct
     1621 gctccagatc ctggaggagg ggaagctcac cgactcgcag gggcggaagg tcgacttccg
     1681 caacacgatc gtgatcatga cgtcgaacat cggcgccggc cagatcgcga agaaccagac
     1741 gctcgggttc tcgttcggcg acgaggtcgg cctctcgtac gacgagatga agggccgcgt
     1801 catgggcgac ctgaagaagg tcttccggcc ggagctcctg aaccggatcg acgagacgat
     1861 cgtcttccac aagctcggca aggaggagat cttcacgatc gtcgacctgc agctgaagcg
     1921 gctgcgcgag cagatggccg cctacgaggt cgcgatcgag ctcaccgacg cggcgaagga
     1981 cgtgctcgtc gagcaggggt tcgacccggc gatgggcgcc cggccgctgc gccgtgcgat
     2041 ccagcgcttc atcgaggacc cgcttgcgga cttcgtgctc ggccgcgagc tcaaccccgg
     2101 cgcgacgatc ctcgtcgacc gcaaggagga gctcacgggc gaggagaacg agtcgctcgt
     2161 cgagatccgc atcgtcgagg gcgaggcgtc gggggagaag gtgacggtgc cggccgccga
     2221 gccggccgag cccgacgagg cgccggcaga cgaggagtaa ccgggaggcc gccgcgaggc
     2281 ggcctctctc gttcttcggc tccgtcctgg cgccgggaag acttttcaca cactcggcac
     2341 agacgtcgtc ccccggcgcc cgtatcgtgg gtcgccgatg gcgaaggcgg cggtcaacta
     2401 cgcgtgcacc gagtgcggct actccgcggg gcgctggttc ggcaagtgcc ccggctgcaa
     2461 cgcgttcggc tcgctcgtcg aggagctgcc gccggacgcc gcccgcccgc aggcga
//
LOCUS       DATLGG010000096         4622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_133373, whole genome shotgun sequence.
ACCESSION   DATLGG010000096 DATLGG010000000
VERSION     DATLGG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133373"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VM638_02700"
     CDS             <1..402
                     /locus_tag="VM638_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVM11374.1"
                     /translation="TTNGSGYEEVPIGDLIRGIAEDASTLVRKELDLAKQEVTEGIKG
                     RAIGLGMLAGAAILGIIALTFVGSTIAYALDNVMPAWLSRLIVTLLYLGGAAGVAVAG
                     TKAMKRGSLTAETARETMKEDVRWAKEQLTR"
     gene            399..779
                     /locus_tag="VM638_02705"
     CDS             399..779
                     /locus_tag="VM638_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11375.1"
                     /translation="MKEIEEVRGRLGDQVEELESRLPAIARTPRRALGFLAATGAAGA
                     ALWVVAGRIRQARRPVGRIERVARKLPDRIANPAMRGIERAPSWTGWALAGAGFLAAT
                     RIAEMRRLKRIERAIQADLADVGR"
     gene            complement(776..1141)
                     /locus_tag="VM638_02710"
     CDS             complement(776..1141)
                     /locus_tag="VM638_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVM11376.1"
                     /translation="MEEHANARRFRQAMEGSGDATGFQDLLADDVVWHEIGGNEPIHA
                     KQAVMDSLAGWIGDVEGQVHDVLANDDHVVAMVEAVARRGDEELRYRTVEVMDMHGGK
                     VVERWSFTEDPAAVTRFFS"
     gene            complement(1207..2277)
                     /gene="mnmA"
                     /locus_tag="VM638_02715"
     CDS             complement(1207..2277)
                     /gene="mnmA"
                     /locus_tag="VM638_02715"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVM11377.1"
                     /translation="MASVLVAMSGGVDSSVAACLLHEAGHEVLGSHLKLVDGASTDHG
                     CCGPQAETDARAVAEHAGFAFETPDLRGRFADHVLDDFVSEHEQGRTPNPCVRCNQHI
                     KFGALIERADELGIDLVATGHYVRSDRGDDGRWRLLRGRDRAKDQSYMVHTLGQRELA
                     RVVFPIGGQTKEETRAHARRLGLPVADKPDSQELCFAPSGDAGGFLRTHAPHLLREGD
                     VVSPDGRPLGTHAGAALYTVGQRRGVGVSTGERSYVLEVDPRANRVVVGPGEMLARRG
                     FEGDRVTWVAGEPPGGALKVEVQIRYRGSAVPAMLLASSERGSARVEFDAPQRAVAPG
                     QSAVFYRGDEVLGGARIKRAFP"
     gene            2303..2953
                     /locus_tag="VM638_02720"
     CDS             2303..2953
                     /locus_tag="VM638_02720"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVM11378.1"
                     /translation="MRLVDLSHILEDGMDAYPGLGVARFTPIVDHETSRDRYRGKAEF
                     YLGHVDMPANTGTYLDSPFHRFPDGDDLGALSLGRVAGLPGIRVRVPGTTQAVEIHLE
                     PRDVRGRAVVVETGRAQGWGTPGYWEDMPYLSTQAIDLLLEGQAALVAVDFGNVDDTD
                     DPVRPAHTRLLEAGVLIVENLADPSDLPDDGFRFFAVPPRIVSGPSFPVRAFAEVE"
     gene            2958..4187
                     /locus_tag="VM638_02725"
     CDS             2958..4187
                     /locus_tag="VM638_02725"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVM11379.1"
                     /translation="MDVRIGPITEEEYEPFMQAAGFAFGRHLSARDLEREQLVFERER
                     ALAARVDGLTVGTAMANSMRLSVPGGEAATAAITSIGVVPTHRRRGIMRELMRRLLEE
                     ARDRGEPLAALHTSEGPIYGRFGFGLATRMGILRIEGRHPEIDVATEPREIQALSRED
                     ALAAFPAIHDGVRQVRPGMPDRSEAWWEYRLRDMEEDEENAQTGYPEPFFALSTGPEG
                     PDGYVIYQVKRDWSDGFPNGTLEVVELMGIDAHAEAALWRYSFGIDLVARTVAWIRPT
                     DDPLLHMLVDPRAARFELRDAVWLRLVDVGAALAARRYGARDRIVIEVRDPFCAWNEE
                     RFELEGGPEGAECRPGAGPADLALSATDLGAIYLGGTRLATLHRAGRVEELNDGAVRR
                     ADAMFAWDPAPWCPMVF"
     gene            complement(4197..>4622)
                     /locus_tag="VM638_02730"
     CDS             complement(4197..>4622)
                     /locus_tag="VM638_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HVM11380.1"
                     /translation="SRTRVKEMRAGNSSKLDLGEAPIVVAGGRGLKSQENFRLVEELA
                     DVLGAGVGASRAAVDAGWYPHQHQVGQTGKTVSPQLYVAVGISGAIQHRAGMQTSKTI
                     VAINKDGDAPIFQLADFGLVGDLFKIVPALTEELKKRKG"
BASE COUNT          665 a         1625 c         1661 g          671 t
ORIGIN      
        1 acgacgaacg gcagcggcta cgaggaggtt ccgatcggcg acctcatccg tgggatcgcg
       61 gaagacgcca gcacgctcgt gcgcaaggag ctggacctgg cgaagcagga ggtcaccgag
      121 ggcatcaagg gccgggcgat cggcctcggg atgctcgccg gggcggcgat cctggggatc
      181 atcgcgctca cgttcgtcgg ttccacgatc gcgtatgccc tcgacaacgt catgcccgcg
      241 tggttgtcac ggctgatcgt gaccctgctc tacctcggcg gcgcggcggg agtcgccgtc
      301 gccgggacga aggcgatgaa gcgtggctcc ctcaccgcgg agaccgcgcg agagacgatg
      361 aaggaggatg ttcgatgggc caaagagcaa ctgacacggt gaaggagatc gaagaggtgc
      421 gcggccggct cggcgatcag gtcgaggagc tcgagagccg gctgcccgcg atcgcgcgga
      481 ccccacggcg ggcactcggg ttcctggccg cgaccggcgc ggcgggtgcg gccctgtggg
      541 tcgtcgccgg ccggatccgc caggcgcggc ggcccgtggg gcggatcgag cgcgtggctc
      601 ggaagctccc cgatcggatc gccaacccgg cgatgcgcgg gatcgagcgc gccccgtcgt
      661 ggaccggctg ggcgctcgcc ggtgccggct tcctcgcggc gacgaggatc gccgagatgc
      721 ggcgcctgaa gcggatcgag cgggcgatcc aggccgatct cgccgacgtc gggcgttagg
      781 agaagaagcg cgtcacggcg gcggggtctt cggtgaagct ccatcgctcg acgaccttgc
      841 cgccgtgcat gtccatgacc tcgaccgtgc gatagcggag ctcttcgtct ccgcggcgcg
      901 caacagcctc gaccatcgcg acgacgtgat cgtcgttcgc gaggacgtca tgtacctggc
      961 cctcgacgtc tccgatccac ccggccaggg agtccatcac ggcctgcttc gcatggatcg
     1021 gttcgttgcc gccgatctcg tgccaaacga cgtcgtcggc gaggaggtcc tggaaccccg
     1081 tcgcgtcacc gctgccttcc atcgcctgcc ggaaccgtct cgcgttcgcg tgctcttcca
     1141 tcgcgcctcc cccgggtcgt cggcgcgagc atagcgagcc gctggacgag gtggaagagg
     1201 tccctctcag ggaaaggcgc gcttgatcct tgccccgccg aggacttcgt cgccccggta
     1261 gaacaccgcg ctctgtcccg gcgcgaccgc ccgctggggc gcgtcgaact cgacgcgcgc
     1321 ggagcctcgt tcgctggagg caaggagcat cgcagggaca gccgaacctc gataccggat
     1381 ctggacctct accttgaggg caccaccggg cggctcgccc gccacccagg tcacgcggtc
     1441 gccttcgaac cccctcctgg cgagcatctc cccgggcccc acgacgacgc ggttcgccct
     1501 cggatcgacc tccaggacgt aggagcgctc ccccgtcgag acgccgacac cgcggcgctg
     1561 ccccaccgtg tagagcgcgg ccccggcgtg ggtccccagc ggccggccgt ccggcgagac
     1621 gacgtcgccc tcgcgcaaca ggtgcggcgc gtgcgtgcgg aggaaccccc cggcgtcgcc
     1681 cgagggcgcg aagcacagct cctgggaatc cggcttgtca gcgaccggca acccgagccg
     1741 ccgcgcatgc gcccgggtct cctccttcgt ctgcccgccg atcgggaaca cgacccgcgc
     1801 gagctcccgc tgaccgagcg tgtggaccat gtaggactgg tccttcgccc ggtcacgccc
     1861 gcgcaggagc cgccagcggc cgtcgtcgcc acggtcggac cggacgtagt ggccggtcgc
     1921 gaccaggtcg atccccagct cgtcggcccg ttcgatcagc gcgccgaact tgatgtgctg
     1981 gttgcagcgc acgcaggggt tcggggtcct tccctgctcg tgctcggaga cgaagtcgtc
     2041 cagcacgtgg tcggcgaacc ggccgcggag gtcgggagtc tcgaacgcga agcctgcgtg
     2101 ctcggccacg gcgcgcgcgt cggtctcggc ctgcggcccg cagcagccgt ggtccgtgga
     2161 ggcgccgtcg acgagcttga ggtgcgagcc cagcacctcg tggcccgcct catgcaggag
     2221 gcaggcggcg accgaagagt cgacgccgcc gctcatcgcg accaggaccg atgccatgca
     2281 tccaggctag catcgagggg tcatgcgact cgtcgacctg agccacatcc tcgaggacgg
     2341 gatggacgcc tacccgggcc tcggcgtcgc ccggttcacg ccgatcgtcg accacgagac
     2401 ctctcgcgac cgctaccggg ggaaggccga gttctacctc ggccacgtcg acatgcccgc
     2461 caacaccggc acgtacctcg actcgccgtt ccaccgtttc cctgacggcg acgacctcgg
     2521 cgccctgtcg ctcggccggg tggccgggct ccccgggatc cgcgtccgcg tcccgggaac
     2581 gacgcaggcg gtcgagatcc acctcgagcc gagagacgtg cgcggccggg cggtcgtcgt
     2641 cgagacgggg cgggcacagg gctggggaac gccggggtac tgggaggaca tgccctacct
     2701 ctcgacgcag gcgatcgacc tgctcctcga gggccaggca gcgctcgtgg ccgtcgactt
     2761 cggcaacgtc gacgacaccg acgatcccgt tcgtcccgcg cacacgcgcc tgctcgaggc
     2821 cggcgtgctc atcgtcgaga acctcgccga cccgagcgac cttcccgacg acggcttccg
     2881 cttcttcgcc gtcccgccga ggatcgtctc ggggcccagc ttcccggttc gggcgttcgc
     2941 ggaggtcgag tagcgccgtg gacgttcgca tcgggccgat caccgaagag gagtacgagc
     3001 cgttcatgca ggcggccggg ttcgccttcg gccgtcatct gagcgcgcgg gacctggagc
     3061 gggagcaact ggtcttcgag cgcgagcggg ccctcgcggc acgggtggac ggcctgacgg
     3121 tcggcaccgc gatggcgaac agcatgcggc tgagcgtccc gggcggggag gccgccaccg
     3181 ccgcgatcac gagcatcggc gttgtcccga cgcaccggcg ccgcggcatc atgcgcgagc
     3241 tgatgcgccg cttgctggag gaggcccggg accgcgggga gcccctcgcc gcgctccaca
     3301 cgtcggaggg gccgatctac ggacgcttcg gcttcgggct cgcgaccagg atggggatcc
     3361 tgcggatcga agggcggcat ccggagatcg acgtcgcgac ggagcctcgc gagatccagg
     3421 cgctgtcgcg ggaggacgcg ctcgcggcgt tcccggcgat ccacgacggc gtccggcagg
     3481 tccgcccggg catgcccgac cggagcgagg cctggtggga gtaccggctc cgcgacatgg
     3541 aggaggacga agagaacgcg cagacgggat atccagagcc cttcttcgcg ctgtcgacgg
     3601 ggcccgaagg gcccgacggc tacgtgatct accaggtgaa gcgcgactgg tccgacggct
     3661 tcccgaacgg aacgctcgag gtcgtcgagc tcatggggat cgatgcccac gccgaggcgg
     3721 cgctgtggcg gtactccttc ggcatcgacc tggtggcgcg gacggtggcc tggatccggc
     3781 ccacggacga tccgctcctg cacatgctgg tcgatcctcg tgcggcgcgg ttcgagctgc
     3841 gggacgcggt ctggctccgg ctcgtcgacg tcggcgccgc gctcgcggcg cgtcgctacg
     3901 gcgcccggga ccggatcgtg atcgaggtgc gggatccgtt ctgcgcgtgg aacgaggagc
     3961 ggttcgagct cgagggcgga cccgaagggg cggagtgccg gccgggcgcg ggtccggccg
     4021 atctcgcgct ctcggcgacc gacctcggcg cgatctacct cggcgggacg cgcctggcca
     4081 cgctgcaccg tgctggacgc gtcgaggagc tcaacgatgg ggcggtccga cgcgccgacg
     4141 cgatgttcgc ctgggacccg gcgccgtggt gcccgatggt tttctgaggc gagggcctag
     4201 cccttgcgct tcttcagctc ctcggtgagc gcgggcacga tcttgaagag gtccccgacc
     4261 aggccgaagt ccgcgagctg gaagatgggc gcgtcaccgt ccttgttgat ggccacgatc
     4321 gtcttcgagg tctgcatccc cgcacggtgc tggatcgcac cggagatgcc gacggccacg
     4381 tagagctggg gcgagacggt cttccccgtc tgaccgacct ggtgctggtg cgggtaccag
     4441 cccgcgtcga ccgccgcgcg cgacgcgccg acccccgcac cgaggacatc cgccagctcc
     4501 tcgacgaggc ggaagttttc ctgcgatttg agcccgcggc cgccggcgac cacgatcggt
     4561 gcttcaccga ggtccagctt cgaagagttg ccggcacgca tctccttcac gcgcgtgcgc
     4621 ga
//
LOCUS       DATLGG010000097         3151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_133468, whole genome shotgun sequence.
ACCESSION   DATLGG010000097 DATLGG010000000
VERSION     DATLGG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133468"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VM638_02735"
     CDS             <1..1500
                     /locus_tag="VM638_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HVM11381.1"
                     /translation="PTNRPMVRLDHQDVIYKTEDAKWNAVVEDLAQRHEKGQPVLVGT
                     VSIEKSEKLSTLLNRRGVPHHVLNAKHHEKEATIIAQAGRRGAVTVATNMAGRGVDIM
                     LGGNAEYLARQEMAANGWDNDRYLLFEMDEEERAAYEAEYQPIFERHKKEIDEAHEEV
                     KQLGGLYVLGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLMRLFASDRISGI
                     MERLKWPEDEAITAKMVSRAIESAQRQVEGVNFERRKNVLKYDEVMNTQRKIVYRDRR
                     RILEGQDLREQTVDMITGYLSALVANYVNPEIFPEEWDLEALLTQLHATYPSDLRKTD
                     LEGISDPEEVNAKVIEEAIAAYDAREEELGTDVMRQLERMVLLSVIDTKWREHLYEMD
                     YLQEGIHLRAYGQRDPLVEYQREAHTMFEQMKGTIEEEFVRYIYRVQLVRQDEPARPR
                     PQRLVTSHGDQPVESPGQAGQAVSGKVPRNAPCPCGSGKKYKKCHGLSA"
     gene            1520..2621
                     /gene="prfB"
                     /locus_tag="VM638_02740"
     CDS             join(1520..1582,1584..2621)
                     /gene="prfB"
                     /locus_tag="VM638_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HVM11382.1"
                     /translation="MSEFTDRIREMSSRLASAKEYFDVDRKRAEAAELEGRLSEPGVW
                     DDPSRGQQLTTSLARLRQPVDRYDRLAERVEELEVLDELLEEGGEEIRSELEEALRRV
                     EADVGKLELASLLSGEFDANDAIASIHAGAGGTESQDWAEMLLRMYLRWAEREGYDAE
                     VDEVTPGEEAGIKSATITLRGPNAYGVVSTEAGVHRLVRISPFDASSRRHTSFASLDV
                     IPLVEEDDEIEIDPKDLRVDTYRSSGAGGQHVNVTDSAVRITHLPTGIVAACQNERSQ
                     MQNRAVAMRILKARLAELQRQQRAERIEDLRGERRDIDFGSQIRSYVLHPYRIVKDHR
                     TGVEIGDVDRVLDGDLEPLIEAALRHRAGAAS"
     gene            2621..>3151
                     /locus_tag="VM638_02745"
     CDS             2621..>3151
                     /locus_tag="VM638_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11383.1"
                     /translation="MALVDERVARAPSPQAPVARRREWLPTLLVVAAALGAAWVWLGP
                     FILHPERLPPGPDMAWYQWRAELLTAHPPATMVLADGPLHVFGGGYRIVSPVLGALVQ
                     QLTGVDEYSVSYALIAGRQVLLGLALGALAYRLRRDAWVFVATVATVTAVLHLRPFVG
                     YVDNVIALTFATGALWF"
BASE COUNT          539 a         1091 c         1093 g          428 t
ORIGIN      
        1 ccgacgaacc gtcccatggt ccgcctcgac caccaggacg tgatctacaa gaccgaggac
       61 gcgaagtgga acgcggtcgt ggaggatctc gcacagcggc acgagaaggg tcagccggtc
      121 ctcgtcggca ccgtctcgat cgagaagtcc gagaagctgt cgacgctcct gaatcgccgc
      181 ggggtcccgc accacgtgtt gaacgcgaag caccacgaga aggaggcgac gatcatcgcc
      241 caggccggcc gtcgtggcgc ggtgacggtg gcgacgaaca tggccgggcg cggcgtcgac
      301 atcatgctcg gcgggaacgc ggagtacctc gcgcggcagg agatggcggc gaacgggtgg
      361 gacaacgacc gctacctcct gttcgagatg gacgaagagg agcgcgccgc gtacgaggcc
      421 gagtaccagc cgatcttcga gcgccacaag aaggagatcg atgaggcgca cgaggaagtg
      481 aagcagctcg ggggcctcta cgtcctcggc accgagcggc acgagtcgcg gcggatcgac
      541 aaccagctcc gcgggcggtc gggtcggcag ggcgacccgg gcgaatcccg cttctacctc
      601 tcgctcgagg acgacctcat gcgcctgttc gcctccgacc gcatcagcgg gatcatggag
      661 cgcctgaagt ggcccgagga cgaggcgatc accgcgaaga tggtctcccg cgcgatcgaa
      721 tcggcccagc gccaggtcga gggcgtgaac ttcgaacgcc gcaagaacgt cctgaagtac
      781 gacgaggtca tgaacaccca gcggaagatc gtctatcggg accgccggcg gatcctcgag
      841 ggccaggacc tgcgcgagca gacggtcgac atgatcaccg gctacctctc tgccctcgtc
      901 gccaactacg tcaaccccga gatcttcccg gaggagtggg acctcgaggc gctcctcacc
      961 cagctccacg ccacctaccc gagcgacctg cgcaagaccg acctcgaagg gatctccgat
     1021 cccgaggagg tcaacgcgaa ggtcatcgag gaggcgatcg ccgcgtacga cgcgcgcgag
     1081 gaggagctcg gcaccgacgt gatgcgtcag ctcgaacgga tggtcctgct gtccgtcatc
     1141 gacacgaagt ggcgcgagca cctctacgag atggactacc tccaggaggg catccacctc
     1201 cgcgcctacg ggcagcggga cccgctcgtc gagtaccagc gtgaggccca cacgatgttc
     1261 gagcagatga agggcacgat cgaagaggag ttcgtgcgct acatctaccg cgtccagctc
     1321 gtgcgccagg acgagccggc tcgcccgcgg ccccagcgcc tcgtcacctc gcacggcgac
     1381 cagccggtcg agtcgcccgg gcaggccggg caggcggtgt cggggaaggt tccccgcaac
     1441 gccccctgcc cctgcgggtc gggcaagaag tacaagaagt gccacgggct gtccgcctga
     1501 gcccggctag gatgccggca tgagcgagtt caccgaccgg atccgggaga tgtcctcccg
     1561 cctcgcgagc gcgaaggagt acctttgacg tcgaccgcaa gcgcgccgag gccgcggaac
     1621 tcgagggtcg cctctccgag ccgggcgtct gggacgaccc ctcgcgcggg cagcagctga
     1681 ccacctcgct cgcccgcctc cgccagcccg tcgaccgcta cgaccgcctc gccgagcgcg
     1741 tcgaggagct cgaggtgctc gatgagctgc tcgaggaagg aggcgaggag atccggagcg
     1801 agctcgaaga ggcgctccgc cgcgtcgagg ccgacgtcgg gaagcttgag ctcgcctcct
     1861 tgctctccgg tgagttcgac gcgaacgacg cgatcgcgag catccatgcg ggcgccggcg
     1921 ggacggagtc gcaggactgg gccgagatgc tgctgcggat gtacctgcgc tgggcggagc
     1981 gcgaaggcta cgacgccgag gtcgacgagg tgactcccgg cgaggaggct ggcatcaaga
     2041 gcgccacgat caccctccgc gggccgaacg cgtacggggt cgtctcgacc gaggcggggg
     2101 tccaccggct cgtgaggatc tcaccgttcg acgcgtcgag ccggcggcac acctccttcg
     2161 ccagtctcga cgtcatcccc ctcgtggagg aggacgacga gatcgagatc gacccgaagg
     2221 atctccgcgt cgacacgtac cgctcgagcg gagccggggg ccagcacgtg aacgtgaccg
     2281 actcggcggt gcggatcacc cacctgccga ccggcatcgt cgcagcctgt cagaacgagc
     2341 ggtcgcagat gcagaaccgc gccgtcgcga tgcggatcct caaggcacgc ctcgccgagc
     2401 tccagcgcca gcagcgcgcc gagcggatcg aggacctgcg tggcgagcga cgggacatcg
     2461 acttcgggtc gcagatccgc tcctacgtcc tgcacccgta tcgcatcgtg aaggatcacc
     2521 ggacgggcgt ggagatcggc gacgtcgacc gcgtgctcga cggcgacctc gagccgctga
     2581 tcgaagcggc gctgcggcac cgagccgggg ccgcgtccta gtggcgctcg tcgacgaacg
     2641 cgtcgcacgc gcgccgtccc cgcaggcgcc cgtcgcgcgt cgccgcgaat ggctcccgac
     2701 cctgctcgtc gtggcggccg ccctgggcgc ggcctgggtc tggctcggcc cgttcatcct
     2761 ccaccccgag cgcctgcccc cgggcccgga catggcgtgg taccagtggc gcgccgagct
     2821 gctgaccgcg cacccgccgg ccacgatggt cctggcggac ggcccgctgc acgtcttcgg
     2881 cgggggatat cggatcgtga gcccggtcct gggcgcgctg gtgcagcagc tcacgggcgt
     2941 cgacgagtac agcgtctcct acgcgctgat cgccggtcga caggtcctgc tcggcctcgc
     3001 gctcggggcg ctcgcgtacc gtctccgacg cgatgcgtgg gtcttcgtcg cgaccgtcgc
     3061 gaccgtcacg gccgtgctgc acctgcggcc attcgtcggc tacgtcgaca acgtgatcgc
     3121 gctgacgttc gcgaccggag ccctgtggtt c
//
LOCUS       DATLGG010000098         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_39136, whole genome shotgun sequence.
ACCESSION   DATLGG010000098 DATLGG010000000
VERSION     DATLGG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39136"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..389
                     /locus_tag="VM638_02750"
     CDS             6..389
                     /locus_tag="VM638_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVM11384.1"
                     /translation="MERIPILKMGEYLLVTIQVDMHDRLAMTLQDDLTSRIVSARAKG
                     VLIDISALEIVDSFIGRTLADIAAMSRVLDAETVVVGMRPAVAITLVELGLSLQGVRT
                     ALNVERGMALLRALIRERLGDDGPA"
     gene            373..776
                     /locus_tag="VM638_02755"
                     /pseudo
     CDS             373..776
                     /locus_tag="VM638_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
     gene            773..1801
                     /locus_tag="VM638_02760"
     CDS             773..1801
                     /locus_tag="VM638_02760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HVM11385.1"
                     /translation="MNVATAFAVGDPSQVSEPRRAVQTLAARAGLSDEQIGRAALVVS
                     ELATNLAKHARGGEMLMRPLTSPSGELDGVEVLALDKGPGIPDVALSRRDGYSTTGTL
                     GHGLGAIERQADWFDLYTHPSGTAIAARLWGTPPDRRRLPRMDVAAAHVAKSGEPICG
                     DGWTWRMRDDRLAIFVADGLGHGMAAHDAADAAVRVFEQYHEEAPARVVADVHAAIRN
                     TRGAAVAALAVDLARGVGRFAGVGNIGGTILLAAGGAHRMVSHPGTAGHTAGRIQEFT
                     YPVPPGSIVVLFSDGLGTQWNLSAYPGIQGRSASVIAGVLYRDYSRRRDDVTVVVAKD
                     RPAVAEKL"
BASE COUNT          298 a          706 c          752 g          276 t
ORIGIN      
        1 gacgcgtgga acgcatcccg atcctcaaga tgggcgagta cctgctcgtc acgatccagg
       61 tggacatgca cgaccgcctg gccatgacgc tgcaggacga cctgacctcc cgtatcgtga
      121 gcgcgcgggc caagggggtg ctgatcgaca tctccgccct ggagatcgtc gactcgttca
      181 tcggccggac gctcgccgac atcgccgcga tgtcccgcgt gctcgacgcc gagacggtcg
      241 tcgtggggat gcgtcccgcg gtcgcgatca cgctcgtcga gctcgggctc tcgctccagg
      301 gagtccgcac cgcgctcaac gtggagcgcg gcatggcgct cctccgcgca ctcatcaggg
      361 aacggctggg cgatgacggt cctgcttagc gagcagcgcg tggtgcagac ggcggaggac
      421 gtcgtcggga tccgccagac ggtccgccag cgcgccgtgg agctcgggtt caacctcgtg
      481 gaccagacga agatcgtcac cgcggcgagc gagctcgcgc ggaacacggt gcagtacggc
      541 ggcggggtcg cgtcaccatc gagggggtcg aagcgttcgg caggcggggg ctgcgcctca
      601 cgttcgagga cgagggtccc ggcatccgcg acgtggagct cgcgatgcgc gacggctaca
      661 ccacgggcgg cgggctcggc ctcgggttga gcggcgcgcg gcgcctgtcg aacgaattcc
      721 acatcgaatc ggccccggga cgagggaccc gcgtcaccat cacccggtgg cgatgaacgt
      781 cgccaccgcg ttcgccgtcg gagatcccag ccaggtctcc gagccgcgcc gggcggtgca
      841 gacccttgcc gcgcgcgcgg ggctgtccga cgagcagatc ggccgggccg cgctcgtcgt
      901 gtccgagctg gccaccaacc tcgccaagca cgcccgcggc ggagagatgc tcatgcgccc
      961 cctgacgagc ccgtccgggg agctggacgg cgtcgaggtg ctggcgctcg acaagggccc
     1021 gggcatcccg gacgtcgcgc tctcgcggcg cgacgggtac tcgaccacag gaacgctcgg
     1081 ccatggcctc ggcgcgatcg aacggcaggc cgactggttc gacctctaca cccacccctc
     1141 cggcaccgcg attgcggccc ggctgtgggg cacgccgccc gaccggcggc ggctgccccg
     1201 gatggatgtc gcggcggcgc acgtcgcgaa gtccggcgaa ccgatctgcg gcgacgggtg
     1261 gacgtggcgg atgcgggacg atcgtctcgc catcttcgtc gcggacggcc tcggccacgg
     1321 gatggccgcg cacgacgccg cggatgcggc ggtccgcgtg ttcgagcagt accacgaaga
     1381 agcgcccgcg cgggtcgtcg ccgacgtgca cgccgcgatc cggaacacgc gcggggccgc
     1441 ggtggcggcg ctcgccgtgg atctcgcacg gggcgtcggg cgcttcgccg gggtcggcaa
     1501 tatcggagga acgatcctgc tggccgccgg cggcgcgcac cggatggtgt cccatcccgg
     1561 gacggccggg cacaccgcgg ggcggatcca ggagttcacc tacccggtgc ccccgggctc
     1621 gatcgtcgtc ctgttctccg acgggctggg cacgcagtgg aacctgagcg cctacccggg
     1681 gatccagggg cgctcggcga gcgtcatcgc cggcgtgctc taccgggatt acagccggcg
     1741 gcgggacgat gtgaccgtcg tggtcgcgaa ggatcgcccg gcggttgccg aaaagctgta
     1801 gcgtactgct ctcccgcgga cgcggtggcc agctcgacgg gacaaggggc agcatttgcc
     1861 actacaatgg ggcccatcgc tcgggcacag cgattgatgt ggatccagaa gccatgccca
     1921 tggcgcagcc acttcggaac cgcgatgccg agaccggcga cgctccgcgc acgaccgcgg
     1981 agctacgggt gtgggtgcgc gagcacctgg gcctctccga ggaccgcgcg gc
//
LOCUS       DATLGG010000099         8164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_147446, whole genome shotgun sequence.
ACCESSION   DATLGG010000099 DATLGG010000000
VERSION     DATLGG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147446"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VM638_02765"
     CDS             <1..305
                     /locus_tag="VM638_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11386.1"
                     /translation="MTELAERLASGPITLEVGVEDEGHDVEGALAGVAQVEGAERSDR
                     DPRLWRIHLASDVRADVARRLVERGIQLRQLRSEADDLDEIYRRYFSEETADVAAG"
     gene            289..1317
                     /locus_tag="VM638_02770"
     CDS             289..1317
                     /locus_tag="VM638_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM11387.1"
                     /translation="MSLPGSAKVERGPSFADRLRTWRERGTPGWAIVARKELADHLLS
                     VRFTVLVAILGLVAIGATYAVASGIRDAATEASEVPALFLRLFTFEPAEGVPSFLSMV
                     GFLAPLLGIAFGFDAVNGERAQGTLPRLVSQPIHRDDVINGKFVAGLAVIGATLLALM
                     LVIAGIGLLRLGIVPSGADIWRMLFWWALTLVYVGFWLGLAVLCSVALRRAATSALVA
                     IASWLVLTLFAGILFGLAADLLSPLPEQPLPQEELANARMEQRLQRLSPTTLYEEATL
                     ALLRPEIKSFRLQLFNPLDQSNIPTGTLSLDQSLIVALPQTVGLVALTVLLFAAAYVL
                     FMRQEIRA"
     gene            complement(1330..1761)
                     /locus_tag="VM638_02775"
     CDS             complement(1330..1761)
                     /locus_tag="VM638_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HVM11388.1"
                     /translation="MQLSETAARKVREIATQQGHPEAALRIAAEPGGCCGYRYSMALV
                     GGVEPGDVAEEHHGVRVVTDPNSAPILAQAEIDFVDTLQQTGFAIHNPTASRGHGHGH
                     GHAGNGGGCGCGRHGGGHGHGEAQGETQKAGHGAGGCACGH"
     gene            complement(1815..2711)
                     /locus_tag="VM638_02780"
     CDS             complement(1815..2711)
                     /locus_tag="VM638_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HVM11389.1"
                     /translation="MRRRLLRPDFPLDLRLTLAHLPRGWQDPTTKLGPDGFWRATRTP
                     DGPATVRVRRADGGIEVLAWGAGAEAALEAAPDLLGWSDDPDAFRPSHPLLRDLARRL
                     RGLRMGRTGAMFEALVPSILEQKVAGKDAWRSYAAIVRAWGEPAPGPRGLLLPPPAER
                     LAAMPYHAFHGFGIERRRAGAVVAAGRSASRVEAAARLAPEEARRRLQALPGIGAWTA
                     AEVTRTAMGDPDAVSVGDYHVPHIVSFALAGEARGDDARMLELLEPCRGQRARAVRLL
                     EAGGIRAPRFGPRMPLRSIARM"
     gene            2810..3031
                     /locus_tag="VM638_02785"
     CDS             2810..3031
                     /locus_tag="VM638_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11390.1"
                     /translation="MARCEVCGMDYEKSFQVTMPDQSTHTFDSFECAIHSLAPTCAHC
                     GCRVIGHGTERDGRIFCCSHCAEQVSVGG"
     gene            complement(3028..4956)
                     /locus_tag="VM638_02790"
     CDS             complement(3028..4956)
                     /locus_tag="VM638_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HVM11391.1"
                     /translation="MIDLVGIRKSFGERVLFEDASLRVGARDRLALVGPNGSGKTTLV
                     EMIVGLQQPDAGEIRIPKETALGYLPQETDALRGRTVLEEVLSAGVAMTEAGHRLEVL
                     HRELDEVEDAGERDRLLAEYADLQSRFETLGGYSLEAEASKICAGLGFREQDLGKRTE
                     ELSGGWMMRVALAKLLLANPDCLLLDEPTNHLDLESVVWLEGFLRGYEGAVVLISHDR
                     DVMNGVATRVVEVEERRLVSYTGNFEAFVQQRELRAEQAQAAARNQQRKVAQTQRFID
                     RFRYKASKARQVQSRIKQLEKMERVEVPGERRRRMGLWFPTPPRPGRVVVELRDVRFA
                     YGEVEVYCDLNVAVERGQRVALVGPNGAGKSTLLKLIAGALEPQAGERKLGANAAVGY
                     FAQHQVEALEPRNRVVQELQRAITPGAEVRPRDLLGRFLFSGDDVDKPVSVLSGGERT
                     RLALAKLLVSPLNLLCLDEPTNHLDIESRDALEDALEAYEGAIVLITHDRHLIRSVAD
                     RIIEVEAGRVRSFEGDYELYLYRKERENVPEAAPLEAVPSAPRRDAKARRREAAEERA
                     RLGATRDELRKIERGIERESAELQRLEQTLADPEFYRAGGQEVADAVRRHGEVRERLA
                     ALEGAWEREATRLEEAGG"
     gene            5023..6849
                     /gene="typA"
                     /locus_tag="VM638_02795"
     CDS             5023..6849
                     /gene="typA"
                     /locus_tag="VM638_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HVM11392.1"
                     /translation="MPTRDDIRNIAIVAHVDHGKTTLVDAMLWQTGAFRENQEVATRV
                     MDSMDLEREKGITILAKNTSVRYGDTKINIVDTPGHADFGGEVERGLMMVDGVLLLVD
                     ASEGPLPQTRFVLRKALEARLPVILVVNKVDRPDARPAEVVNEVEELFLDLDADEHQI
                     SFPILYASAKAGWVSPGRDQVGTDLEPLFRTLLEHIPAPTYDEDHPLQALVTNLDASP
                     YVGRLALCRILHGSVRRGQDVAWCRADGSVERVKITELYVTRNLERVEAEEAGPGEII
                     AVAGLADVTIGETLSDPEDPRPLPVSRVDEPSLSMTIGINTSPLAGQDGEKLTARLVK
                     ARLEQELVGNVSIRVLDTERPDTWEVQGRGELQLAVLVEMMRREGFELTVGKPQVVTR
                     EVNGKRHEPMERLAIDIPEDYLGVVTQLISLRKGRLEQIVNHGTGWVRMEYIVPARGL
                     IGFRTEFLTETRGTGQLHHVFEGYEPWHGELRTRLSGSLVADRRGPTTTYALLNLSER
                     GEMFVGPGVEVYEGMIVGENARPEDMDVNPTKEKKLTNMRASSADETVRLVPHRQLSL
                     EQALEFLREDECAEVTPKNVRLRKVVLSAQERGKTARGAKRS"
     gene            6864..7652
                     /locus_tag="VM638_02800"
     CDS             6864..7652
                     /locus_tag="VM638_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVM11393.1"
                     /translation="MDERVRELAARYSADAEAYEELWAPELAPLNRALIGDLPLREAG
                     RVLDLGSGVGAHLAVLEEAAPEATIVAADRAEGMIRRAPERFPRVVLDARWLAFADAT
                     FDAAVMAFMLFHVPEPDRALTEVHRVLRPGGSLGLATWGEQRLRAAIEAWTDVLDDAG
                     APEPEPVVALHDLMDTEEAVTGLLQDAGFADITVRTVRSEHPVSLEEFIALRTRIGPS
                     RARLHGLDPSVRSEVVERAIDRIKDMDPREYVEDVDAILAVARA"
     gene            complement(7654..8112)
                     /locus_tag="VM638_02805"
     CDS             complement(7654..8112)
                     /locus_tag="VM638_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HVM11394.1"
                     /translation="MEYIRAAGAEDAEGCIFCDLPPQDDDEKNLILARGEHAFVILNR
                     FPYNAGHLMVAPNRHEGEYEDLSEEEVLEVHRLLQRAIRALKEEMEPHGFNVGMNLGR
                     VAGAGIPGHLHWHVVPRWGGDTNFMPVVGGTKVLPELLEETRDRLAARFA"
BASE COUNT         1126 a         3006 c         2851 g         1181 t
ORIGIN      
        1 cgatgaccga gctggcggag cgcctcgcgt cgggcccgat cacgctcgag gtcggggtcg
       61 aggacgaagg ccacgacgtc gaaggggcgc tcgcaggggt cgcgcaggtc gaaggcgcgg
      121 agcgctccga ccgcgatccg cggctgtggc ggatccacct cgcctccgac gtgcgcgccg
      181 acgtcgcgcg ccggctcgtg gagcgcggca tccagctccg gcagctgcgg agcgaagccg
      241 acgacctcga cgagatctac cgccgttact tctccgagga gaccgccgat gtcgctgccg
      301 ggtagcgcga aggtcgagcg cggcccgtcg ttcgcggacc gcctccgcac ctggcgggag
      361 cgcgggacgc cgggctgggc gatcgtcgcc cggaaggagc tcgccgacca tctgctctcc
      421 gtccgcttca cggtcctggt cgcgatcctc gggctcgtcg cgatcggcgc gacctacgcc
      481 gtcgcgagcg ggatccgcga cgcggccacc gaggcatccg aggtcccggc gctgttcctg
      541 cggctgttca cgttcgagcc ggcggagggc gtgccgtcgt tcctgtcgat ggtcggcttc
      601 ctcgcgccgc tcctggggat cgccttcggc ttcgacgccg tcaacggcga gcgtgcgcag
      661 ggaacgctgc cgcggctcgt ctcgcagccg atccaccgcg acgacgtgat caacgggaag
      721 ttcgtcgccg gactggcggt gatcggcgcg acgctcctcg cgctcatgct cgtgatcgcc
      781 gggatcgggc tcctccggct cggcatcgtg ccgtcaggcg cggacatctg gcggatgctc
      841 ttctggtggg cgctgacgct cgtctacgtg ggtttctggc tcgggctggc ggtcctttgc
      901 tccgtcgccc tccggcgcgc ggccacgtcg gcgctcgtcg cgatcgcctc gtggctcgtg
      961 ctcacgctgt tcgccgggat cctgttcggg ctcgccgcgg acctgctctc gccgctcccg
     1021 gagcagccgc tgccgcagga ggagctggcg aacgctcgca tggagcaacg gctccagcgg
     1081 ctctccccca ccaccctcta cgaggaggcc acgctcgcgc tcctgcggcc cgagatcaag
     1141 agcttccggc tccagctctt caacccgctc gaccagtcga acatccccac ggggacgctg
     1201 tcgctcgacc agagcctgat cgtggcgttg ccgcagacgg tcgggctcgt cgcgctgacc
     1261 gtgctgctgt tcgcggcggc ctacgtgctg ttcatgcggc aggagatccg ggcctgagcc
     1321 cgggcggcct cagtgaccgc aggcgcaccc gccggcgcca tggccggcct tctgcgtctc
     1381 gccctgggcc tccccgtggc cgtggccgcc gccgtgacgc ccgcagccgc agccacctcc
     1441 gttgccggcg tggccgtgac cgtgcccgtg accgcggctc gcggtcgggt tgtggatcgc
     1501 gaagcccgtc tgctggagcg tgtcgacgaa gtcgatctcg gcctgcgcga ggatcggcgc
     1561 gctgttcggg tcggtgacga cccggacgcc gtggtgctcc tcggccacgt cgcccggctc
     1621 caccccgccg acgagggcca tcgagtagcg gtagccgcag catccgccgg gctccgcggc
     1681 gatccgcagt gccgcctcgg gatgcccctg ctgggtcgcg atctcccgta ccttccgtgc
     1741 tgcggtctcg ctgagctgca tcctcgtcct cctggggggt ccgtccgacc tcctcatggt
     1801 accccggatc cggctcacat ccgggcgatc gagcggagcg gcatcctcgg gccgaaccgg
     1861 ggggcccgga tcccgcccgc ttcgaggagg cggacggcgc gagcgcgctg gccgcggcac
     1921 ggctccagca gctccagcat cctcgcgtcg tcccctcgcg cctcgccggc gagcgcgaac
     1981 gagacgatgt gagggacgtg gtagtccccg acgctcacgg cgtcggggtc ccccatcgcc
     2041 gtgcgggtga cctcggcagc cgtccacgcg ccgatgccgg gaagcgcctg gagccgccgc
     2101 cgcgcctcct ccggtgcgag gcgcgcggcc gcctccaccc gggaggccga ccgcccggcc
     2161 gcgacgacgg cgcccgctcg ccggcgctcg atcccgaacc cgtggaacgc gtggtacggc
     2221 atggcggcca gccgttcggc cggaggtggg aggagcagcc cgcggggtcc gggcgccggc
     2281 tcgccccacg cgcgaacgat cgcggcatag gaccgccagg cgtccttccc cgccaccttc
     2341 tgctcgagga tgctcgggac gagcgcctcg aacatcgcgc cggtgcggcc catccgcagc
     2401 ccgcggaggc gacgtgcgag gtcgcggagc agcgggtgcg acggccggaa cgcgtccggg
     2461 tcgtcgctcc agcccaggag atccggcgcc gcctcgagcg ccgcttcggc tcccgctccc
     2521 cacgcgagga cctcgatccc cccgtcggcc cgccgcaccc gcaccgtggc cggtccgtcc
     2581 ggcgtccggg tggcacgcca gaagccgtcc ggaccgagct tcgtcgttgg gtcctgccat
     2641 ccgcgcggaa ggtgcgcaag cgtgagccgc aggtcgaggg ggaagtccgg acggaggaga
     2701 cgccgccgca cgcgcgcagg gtaccgcgcc gcgggtggcc gaaccaccgg ggcggtttga
     2761 cggcgcgcgg cctcggttag gaaccggagg acgaccgagg aggtggcaga tggctcgctg
     2821 cgaggtctgc ggcatggact acgagaagtc gttccaggtc acgatgccgg accagagcac
     2881 ccatacgttc gacagcttcg agtgcgcgat ccactcgctg gcgcccacct gcgcgcactg
     2941 cggctgccgc gtgatcggcc acggcaccga gcgggacggc cggatcttct gctgctcgca
     3001 ctgcgccgag caggtcagcg tcgggggcta gccgccggcc tcttcgagcc gcgtcgcctc
     3061 gcgctcccag gcgccctcga gcgcggccag ccgctcccga acctcaccgt ggcggcggac
     3121 cgcgtccgcc acctcctgcc cccctgcccg gtagaactcc gggtccgcga gggtctgctc
     3181 cagacgctgc agctcggctg attcccgctc gatgccgcgc tcgatcttcc gcagctcatc
     3241 gcgtgtggcg cccagccgcg cccgctcctc ggcggcctcg cgccgtcgcg ccttcgcgtc
     3301 cctccgcggt gccgacggaa cggcttcgag cggggcggcc tcgggcacgt tctcgcgctc
     3361 cttgcggtag aggtagagct cgtagtctcc ttcgaaggac cgcacccgcc ccgcctcgac
     3421 ctcgatgatg cggtcggcga ccgagcggat cagatggcgg tcgtgggtga tcagcacgat
     3481 cgcgccctcg taggcctcga gcgcgtcctc gagcgcgtcg cggctctcga tgtcgaggtg
     3541 gttcgtcggc tcgtcgagac acagcaggtt gagcggcgag acgaggagct tcgcgagcgc
     3601 gagtcgcgtt cgctccccac ccgacaggac cgagacgggc ttgtccacgt cgtcgccgga
     3661 gaacaggaac cggccgagca ggtcccgcgg ccgaacctcc gcgcccgggg tgatcgcgcg
     3721 ttggagctcc tgcaccacgc ggttgcgcgg ctcgagcgcc tcgacctggt gctgggcgaa
     3781 gtagccgacc gccgcgttcg cgccgagctt ccgctccccc gcctgcggct cgagcgcgcc
     3841 ggcgatcagc ttgaggagcg tcgacttccc ggcgccgttc ggcccgacga gcgcgacacg
     3901 ctggccccgc tcgaccgcga cgttcaggtc gcagtagacc tcgacctccc cgtaggcgaa
     3961 ccgcacgtcg cggagctcga cgaccacgcg gcccggtcgc gggggcgtcg ggaaccacag
     4021 gcccatccgg cgccggcgct cgccggggac ctcgacccgc tccatcttct cgagctgctt
     4081 gatccggctc tgcacctggc gcgccttcga cgccttgtag cggaagcggt cgatgaagcg
     4141 ctgggtctgc gcgaccttgc gctgctggtt gcgcgcggcg gcctgcgcct gctcggcccg
     4201 cagctcccgc tgctgcacga aggcctcgaa gttcccggtg taggaaacga gccgccgctc
     4261 ctcgacctcg accacgcgcg tcgcgacgcc gttcatcacg tcgcggtcgt gggatatgag
     4321 caccaccgcg ccctcgtagc cgcggaggaa cccctcgagc cagaccaccg actccaggtc
     4381 gaggtggttc gtcggctcgt cgagcaggag gcagtcgggg ttggcgagca ggagcttcgc
     4441 caacgccacc cgcatcatcc acccgccgga gagctcctcg gtccgcttgc cgagatcctg
     4501 ctcccggaat ccaaggcccg cgcagatctt cgaggcctcg gcctccagcg agtagccgcc
     4561 gagcgtctcg aaccgcgact ggaggtctgc gtactccgcc aggaggcggt cgcgctcgcc
     4621 ggcgtcctcg acctcgtcca gctcgcggtg gagcacctcg agccggtgcc cggcctccgt
     4681 catcgccaca ccggccgaga gcacctcctc gaggacggtc cgtccgcgca gcgcgtcggt
     4741 ctcctgcggg aggtagccga gcgcggtttc cttcgggatc cggatctcgc ccgcgtcggg
     4801 ctgctggagg ccgacgatca tctcgacgag cgtggtcttg ccggagccgt tcggccccac
     4861 gagcgccagg cgatcgcgcg cgcccacgcg cagcgacgcg tcctcgaaca gcacgcgctc
     4921 gccgaacgac ttccggatgc cgacgaggtc gatcacgggt cgagcgtacg ggacggtccg
     4981 ctacactgac gatgcaaccc cgaccacctc ccggagaccg ccatgcccac ccgcgacgac
     5041 atccgcaaca tcgccatcgt ggcgcatgtc gaccacggca agacgacgct cgtcgacgcg
     5101 atgctgtggc agacgggcgc cttccgcgag aaccaggagg tcgcgacgcg ggtcatggac
     5161 tcgatggacc tcgagcgcga gaagggcatc acgatcctcg cgaagaacac ctcggtccgc
     5221 tacggcgaca cgaagatcaa catcgtcgac acgccgggcc acgccgactt cggcggcgag
     5281 gtcgagcgcg gcctgatgat ggtcgacggg gtgctcctgc tcgtcgacgc gagcgagggc
     5341 ccgctcccgc agacgcgctt cgtcctccgc aaggcgctcg aggcccgcct gccggtgatc
     5401 ctcgtcgtga acaaggtcga ccggcccgac gcccgccccg ccgaggtggt gaacgaggtg
     5461 gaggagctct tcctcgacct cgacgccgac gagcaccaga tctccttccc gatcctctac
     5521 gcgagcgcga aggccggctg ggtctcccca gggcgcgatc aggtcggcac ggacctcgag
     5581 ccgttgttcc gcaccctgct cgagcacatc ccggcgccca cctacgacga ggaccacccc
     5641 ctccaggcgc tcgtgacgaa cctcgatgcc tccccgtacg tcggtcggct cgcgctctgc
     5701 cggatcctgc acggctcggt gcggcgcggg caggacgtcg cctggtgccg cgccgacggc
     5761 tcggtcgagc gcgtgaagat caccgagctc tacgtgaccc gcaacctcga gcgcgtcgag
     5821 gccgaggagg ccgggcccgg cgagatcatc gcggtggccg gactcgccga cgtcacgatc
     5881 ggcgagacgc tgtcggaccc cgaggacccg cggccgctcc cggtcagtcg cgtcgacgag
     5941 ccgagcctgt cgatgacgat cgggatcaac acctcgccgc tcgccggcca ggacggggag
     6001 aagctcaccg cgcgcctcgt gaaggcgcgc ctcgagcagg agctcgtcgg caacgtgtcg
     6061 atccgcgtcc tcgacaccga gcgtcccgac acctgggagg tccagggccg cggcgagctc
     6121 cagctcgcgg tcctcgtcga gatgatgcgc cgcgaggggt tcgagctcac ggtcggcaag
     6181 ccgcaggtgg tcacgcggga ggtcaacggc aagcggcacg agccgatgga gcgcctcgcg
     6241 atcgacatcc ccgaggacta cctcggcgtc gtgacccagc tcatctcgct ccgcaagggc
     6301 cgcctcgagc agatcgtgaa ccacggcacc ggctgggtgc ggatggagta catcgtgccg
     6361 gcgcgcggcc tgatcgggtt cagaacggag ttcctcaccg agacccgcgg caccggccag
     6421 ctccaccacg tgttcgaggg gtacgagccc tggcacggcg agctgcgcac gcgcctatcg
     6481 ggctcgctgg tcgccgaccg ccgcggcccg accacgacct atgcgctgct gaacctctcc
     6541 gagcggggcg agatgttcgt cggtcccggc gtcgaggtct acgaggggat gatcgtcggc
     6601 gagaacgccc gccccgagga catggacgtg aaccccacga aggagaagaa gctcaccaac
     6661 atgcgagcct ccagcgccga cgagaccgtc cgcctcgtcc cccaccgcca gctctccctc
     6721 gagcaggccc tcgagttcct tcgcgaggac gaatgcgccg aggtcacgcc gaagaacgtc
     6781 cgcctccgca aggtcgtgtt gtcagcccaa gaacgcggca aaacagcccg cggcgccaaa
     6841 cgctcctgac ctacgatgcc cgggtggacg aacgggtgcg ggagctggcg gctcgatact
     6901 cggctgacgc ggaggcgtac gaggagctgt gggcgccgga gcttgcccca ctaaaccgag
     6961 cgctgatcgg ggatcttccg ctgcgtgagg ccgggcgggt gctcgacctg ggaagcggcg
     7021 tcggagcgca cttggcggtc ctcgaagaag cggcaccaga agcgaccatc gtggcggccg
     7081 accgtgccga ggggatgatc cggcgcgcgc ccgagcggtt tccccgggtc gtgctagacg
     7141 cgaggtggct agcgttcgca gacgcgacct tcgacgcggc cgtcatggcg ttcatgctct
     7201 tccacgtccc ggagcccgac cgggccctga cggaggtcca ccgcgtgctg cggccgggag
     7261 ggtcgctcgg gctcgccacc tggggcgagc agcggctgcg ggcggcgatc gaagcctgga
     7321 ccgacgtcct cgacgacgcg ggcgcgcctg agcccgagcc ggtcgtcgcc ctgcacgacc
     7381 tgatggacac ggaggaagcc gtgaccggac tgcttcagga cgcgggcttc gccgacatca
     7441 cggtgcggac cgtgcgctcg gagcatcccg tcagcctcga ggagttcatc gcgctccgga
     7501 cgaggatcgg tccctcccgc gcgcggctgc acggcctcga cccgtcggtc cgctcggagg
     7561 tcgtcgagcg ggcgatcgac cggatcaaag acatggaccc gcgcgagtac gtcgaagacg
     7621 tggacgcgat cctcgcggtc gcgcgggcct gagtcaggcg aacctggcgg cgaggcggtc
     7681 acgggtctcc tcgagcagct cggggaggac cttcgtccct ccgaccactg gcatgaagtt
     7741 ggtgtcgccg ccccagcggg ggacgacgtg ccagtgcagg tggccgggga tgccggcgcc
     7801 ggcgacccgg ccgaggttca tgccgacgtt gaagccgtgg ggctccatct cttccttgag
     7861 cgcgcggatc gctcgttgga gcaatcgatg cacctcgagc acctcctcct ccgacaggtc
     7921 ctcgtactcc ccctcgtgtc ggttcggggc caccatcaga tgcccggcgt tgtaggggaa
     7981 gcggttcagg atcacgaagg cgtgctcgcc gcgcgcgagg atcaggttct tctcgtcgtc
     8041 gtcctgcggg gggaggtcgc agaagatgca gccctccgcg tcctcggcgc ccgcggcccg
     8101 gatgtactcc atccgccacg gcgaccagag gcgctcggtc accccgccag cgctccgatg
     8161 tcgc
//
LOCUS       DATLGG010000100         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_147559, whole genome shotgun sequence.
ACCESSION   DATLGG010000100 DATLGG010000000
VERSION     DATLGG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147559"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VM638_02810"
     CDS             <1..616
                     /locus_tag="VM638_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVM11395.1"
                     /translation="HRYPAFKLFLAYEEPWWRELQISSGRTVTDLPIRQAFYFGVEET
                     ERALLMSSYSDDRTVAYWQGLGWTERDEPTAPRLQVAGAGGPSEALVAPHEMIRHAQA
                     QLARMHGIDVPPPYLAAYRNWGAPPFGGAWHLWRINADSVAIGKRMRRPLPEYNVHVC
                     GEAYSGIQAFIEGALTSTELVLQEELGLEGPAWLPDDYYLGPRA"
     gene            complement(713..2029)
                     /locus_tag="VM638_02815"
     CDS             complement(713..2029)
                     /locus_tag="VM638_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HVM11396.1"
                     /translation="MRARRSNHSLRAILPRAAATVSLLLAIGLFAPAGAGDDEHVKDA
                     KTPLLHEADTLQPVVEEPLGEMRCEEGMAGPFPCENVDLQSFVPITAMAGVTGNDVWG
                     WTDPQTGREYALMGTSHGTTFVDVTEPTQPEIVGTLPTSGIPDFFLWRDIKVYEDHAL
                     IGAEHSSHGLQVFDLTRLRNVPVKPAVFTADTVYRGFGYSHNVAVNEETGYAYAVGSN
                     TCNGGLHMIDVRDPKNPTFAGCFSDDGYTHDVQCVIYRGPDGRFRGREICFASNEDTV
                     TIVDVTDKSNPVMLSRTGYGTSAYTHQGWLTPDHRWFLFGDELDEQGARVPNTTTYIM
                     ELRKLTSPATPVPYFHETQSIDHNMFIRDRLVYQSNYMAGLRILEHDRRSLRSGQLDE
                     VGYFDVVPALDVREFAGTWSNYPYFDSGTVLVSTIESGLFVLRPTV"
     gene            complement(2118..>2572)
                     /locus_tag="VM638_02820"
     CDS             complement(2118..>2572)
                     /locus_tag="VM638_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11397.1"
                     /translation="GEGNGYGELVDEGGADRYEVAAASEATADGGEPETGRVAATSAT
                     SAAADVFAQAAARALGHASLRDTGGSDGYSVAATSRAFAGDGTSSAGVARAGALASVD
                     EGSSASFADADGGEPDVFSADPWIAACTGTRGGDAWVDCGGAGIGVVR"
BASE COUNT          381 a          895 c          886 g          410 t
ORIGIN      
        1 ccaccgctat cccgcgttca agctgttcct ggcgtacgag gagccgtggt ggcgagagct
       61 gcagatcagc agcgggcgga ccgtgaccga cctcccgatc cggcaggcct tctacttcgg
      121 ggtcgaggag accgagcgcg cgctcctgat gagctcctac agtgacgatc ggaccgtcgc
      181 gtactggcag ggcctcggct ggaccgaacg ggacgagccg accgccccac gcctgcaggt
      241 cgcgggcgcc ggagggccga gcgaagcgtt ggtcgcgccg cacgagatga tccggcacgc
      301 gcaggcgcag cttgcccgga tgcacggcat cgacgtaccg cccccgtacc tcgccgcgta
      361 tcggaactgg ggcgcgcctc cgttcggcgg cgcgtggcac ctgtggagga tcaatgccga
      421 ctccgtggcg atcggaaagc gcatgcgccg ccccctgccg gagtacaacg tgcacgtctg
      481 cggcgaggcg tactcgggga tccaggcctt catcgagggg gcgctgacct cgacggagct
      541 cgtgctccag gaggagctcg gcctcgaagg cccggcgtgg ctccccgacg attactacct
      601 ggggccgcgc gcctgacgcg acgatcgccg gatcaggcgg gaggtcgccg aggagatgcg
      661 ccggggcgag gggtctagat ccccccggcg catctccact agcagcgaga tctcagacgg
      721 tcggccggag cacgaacagg ccgctttcga tcgtgctcac gagcacggtc ccggagtcga
      781 agtacgggta gttgctccag gtgcccgcga actcccggac gtcgagagcc ggcaccacgt
      841 cgaagtaacc gacctcgtcc agctggcccg atcggagcga gcgtcgatcg tgctcgagga
      901 tccgcaggcc cgccatgtag ttcgactggt agacgagccg gtcgcggatg aacatgttgt
      961 ggtcgatcga ctgcgtctcg tggaagtacg ggaccggcgt cgccggggac gtcagcttcc
     1021 gcagctccat gatgtaggtc gtcgtgttcg ggacgcgtgc gccctgctcg tcgagctcgt
     1081 cgccgaacag gaaccagcgg tggtccggcg tcagccaccc ctggtgggtg taggccgagg
     1141 tcccgtagcc ggtgcgtgag agcatcaccg ggttcgactt gtccgtgacg tcgacgatcg
     1201 tgacggtgtc ctcgttcgag gcgaagcaga tctcgcggcc ccggaagcgt ccatccgggc
     1261 cgcggtagat cacgcactgc acgtcgtggg tgtagccgtc gtcggagaag cagcccgcga
     1321 acgtcgggtt cttggggtcg cggacgtcga tcatgtgcag gccgccgttg caggtgttgc
     1381 tcccgacggc gtaggcgtag ccggtctcct cattcacggc aacgttgtgc gaatagccga
     1441 atccgcggta gaccgtgtct gccgtaaaga ccgccggctt cacgggcacg ttccgcagtc
     1501 gcgtgaggtc gaacacctga agcccgtggc tcgagtgctc ggcgccgatc agcgcgtgat
     1561 cttcgtacac cttgatgtcg cgccacagga agaaatcggg gatcccgctc gtcggaaggg
     1621 tcccgacgat ctcaggctgg gtcggctcgg tgacgtcgac gaaggtcgtc ccgtggctcg
     1681 ttcccatcag cgcgtactca cgaccggtct gcgggtcggt ccacccccag acgtcgttcc
     1741 ctgtcacccc ggccatcgcg gtgatcggga cgaagctctg gaggtcgacg ttctcgcagg
     1801 ggaacggacc cgccatcccc tcctcgcacc gcatctcacc gagcggttcc tcgacgacgg
     1861 gctggagggt gtccgcctcg tggagcagcg gcgtcttcgc gtccttcacg tgctcgtcgt
     1921 ccccggctcc ggccggggcg aacagcccga tcgcgagcag cagtgagacc gtagccgcag
     1981 cccgggggag gatcgctcgg agggagtggt tcgacctacg tgcacgcatg atgcatcacc
     2041 tcggtatggt cggctcctcc taccctttcc ggagcgcgtc gagcccacac cgaggcaagc
     2101 atgtccgcgc gtcgcggtta tcgcacgacg ccgatccccg cgcccccgca gtcgacccac
     2161 gcgtccccgc cgcgcgttcc ggtgcaggcg gcgatccagg ggtcggcgct gaacacgtcg
     2221 ggttccccgc cgtctgcgtc ggcgaaggat gccgaggaac cctcgtcgac actggcgagg
     2281 gcgcccgccc gggcgacccc cgccgaggac gtgccgtccc cggcgaacgc tcggctggtc
     2341 gctgcgacgg agtacccatc actcccgccg gtgtcgcgga gcgaggcatg tccgagggcg
     2401 cgcgccgcgg cctgcgcgaa cacatcggcc gccgccgatg tcgcggaggt cgccgcgacc
     2461 cgtcccgtct ccggctcgcc gccatcggcg gtcgcctcgc tcgcggcggc cacctcgtag
     2521 cggtcggcgc ccccctcgtc caccagctct ccgtatccgt tcccctcccc cg
//
LOCUS       DATLGG010000101         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_114996, whole genome shotgun sequence.
ACCESSION   DATLGG010000101 DATLGG010000000
VERSION     DATLGG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114996"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /locus_tag="VM638_02825"
     CDS             <1..1925
                     /locus_tag="VM638_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVM11398.1"
                     /translation="HVHSPHDIFFNATGTRAYTASVTHSDILDTTDPTNPRLIASIHD
                     PAINIHHDTQVTPDGKTLIIGDELAGAATGPQCPGGGLHFYDITNEAQPRKIGVFFAD
                     TQDRANLCTAHVFRINPDGKSLVIGWYNGGTRIVDISDPNGLGPHEIAHMVPTGLGGN
                     SGVANSWASKAYKGYVYSNDRARGLDIMQLDAPEPSPGSAQGVVALIDTGINPYHRQF
                     RWDDPRAYQHPSTYIPGYPENAVALPITLDEPNYWAAVRRDCASIWSKVEPGKLYWFP
                     GTKIVGGISFGNVAGMTNCSAAEPGGGRILDTGGHGTMVASRAAGNTYGACRTCLVVA
                     IQNPLAVNLGNPSSSTQEVMDSIRFAGNNAHWIDAQSNSWGPIVFPAWEPTGAAGLIT
                     ANPQLVRAVEEVSQKHPAFWASGNGAAGRGGVIGSPTLLTPTLTPSAISVGGNDAGYV
                     NVWPGFPPHVISDSCQAWAAQHRSTTGENGSVAGGTSGATPFAAGGAVRILLEARRIL
                     GEERTGVRNGVAAEGPAGLVDGPLADGKLTMAEWREVLFKTASSRHIREFEDGNVCAT
                     AAQFNTYPVKYGQIPKQAPAWLIVGYGAIDGPSRRLAYSVLNGERAMPVRTQEDAYFT
                     VDDAARRATHEVYSRP"
     gene            1945..2832
                     /locus_tag="VM638_02830"
     CDS             1945..2832
                     /locus_tag="VM638_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11399.1"
                     /translation="MRRRLLAPIAMIGVLALAAPATGVPVVAISIPEDGATISRSTGS
                     ALQVGGAVEFAVPEKGSRRFYVRRTSCNADQRLSITRGSETAGCAQTVSLTPVAEETA
                     TAVTYAAENGVPFTLDATQPITGTLSLVSYLLVTGNVGVGAGLVTADISVTGAEVGGA
                     EQALGSTTISYTATPATYRVDTPWSIAPPATLDKKDFLGLTLSITVRGRNALHGFTRP
                     NDTNFLVPIWTASFNRRVEVAIDNGSFTSTGISVGPDLTTWSGSRPWPSLGTHVLRAR
                     AVQGGVVSGIDQRAFTITS"
     gene            complement(2892..3476)
                     /locus_tag="VM638_02835"
     CDS             complement(2892..3476)
                     /locus_tag="VM638_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HVM11400.1"
                     /translation="MANELQGKRVAFLATDGVEQVELTEPWQAVMDAGATVELLSPKP
                     DAIQAMNGMEKGAAFEVDRAVADARPDEYDGLILPGGVKNPDLLRMDEASVSFVREFV
                     GSGRPVAVICHGPWTLVEAGVVRGRTLTSYPSVRTDIENAGGTWVDEEVHVDNGLVSS
                     RKPADLPAFCRKLVEEIAEGHHEPAQAGAGAQYA"
     gene            3634..>4377
                     /locus_tag="VM638_02840"
     CDS             3634..>4377
                     /locus_tag="VM638_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11401.1"
                     /translation="MRRLAAATITTVLAVCLLAPPAAAYPRPGKTLRVNPLLDLTGGP
                     ASGGTPQLSGDGRHVLFPTTARLVEDDTGLAVDCYVRDLVTWSLVRASVAHDGTEANG
                     SCSSPAISRDGRYVAWHSTATNLVPGDTNGLADIFVRDLVAGTTTRASVSAQGAQADG
                     ASTSAAISDDGRVVAFLSSATNLVAGDSNAFNDVFVRNMEDGSIQIATLSTEGTQGVT
                     AVSQFELSGNGRHVVFVHRMPWEGDKSSYD"
BASE COUNT          678 a         1619 c         1467 g          613 t
ORIGIN      
        1 accacgtcca ctcgccccac gacatcttct tcaacgccac cgggacgagg gcgtacaccg
       61 catcggtcac gcacagcgac atcctcgaca cgacggatcc cacgaacccg cgtctgatcg
      121 cctcgatcca cgacccggcg atcaacatcc accacgacac gcaggtgacg cccgacggca
      181 agaccctgat catcggtgac gagctcgcgg gcgcggcgac gggcccgcag tgcccgggcg
      241 gcggcctgca cttctacgac atcacgaacg aggcccagcc gcgcaagatc ggcgtcttct
      301 tcgccgacac ccaggaccgg gcgaacctct gcaccgcgca cgtgttccgc atcaacccgg
      361 acgggaagtc cctcgtgatc ggctggtaca acggcggcac gaggatcgtc gacatctccg
      421 accccaacgg gctcggcccg cacgagatcg cgcacatggt cccgaccggg ctcggcggca
      481 acagcggcgt cgcgaactcg tgggcctcga aggcctacaa gggctacgtc tactcgaacg
      541 accgcgcgcg ggggctcgac atcatgcagc tcgacgcgcc cgagccgagc ccgggcagcg
      601 cgcagggcgt ggtggcgctg atcgacaccg ggatcaaccc ctaccaccgc cagttccgct
      661 gggatgaccc ccgggcctac cagcacccgt cgacgtacat cccgggctac ccggagaacg
      721 ccgtcgccct tccgatcacg ctcgacgagc cgaactactg ggccgcggtg cgccgcgact
      781 gcgcctcgat ctggtcgaag gtcgagcccg ggaagctcta ctggttcccc ggcacgaaga
      841 tcgtcggagg gatctccttc ggcaacgtgg ccgggatgac gaactgcagc gccgcggagc
      901 cgggtggcgg gcggatcctc gacaccggcg gccacggcac gatggtggct tcacgtgccg
      961 cgggcaacac ctacggcgcg tgtcgcacgt gcctcgtcgt ggcgatccag aacccgctcg
     1021 cggtgaacct cggcaatcct tcctcgagca cgcaggaggt catggactcg atccgcttcg
     1081 cggggaacaa cgcgcactgg atcgacgccc agtcgaactc ctgggggccg atcgtcttcc
     1141 ccgcctggga gccgacggga gcggcggggc tcatcaccgc caacccccag ctcgtccggg
     1201 ccgtggagga ggtctcacag aagcacccgg cgttctgggc atcgggcaac ggcgcggccg
     1261 ggcgcggcgg cgtcatcggg agcccgaccc tgctgacccc aaccctcacg ccctcggcga
     1321 tcagcgtcgg cgggaacgac gcgggctacg tgaacgtctg gcccggcttc cccccgcacg
     1381 tgatctcgga ttcctgccag gcgtgggccg cgcagcaccg atcgaccacc ggtgagaacg
     1441 gcagcgtcgc gggcgggacg tccggcgcga cgccgttcgc cgcgggaggg gcggtccgga
     1501 tcctgctcga ggcccggcgc atcctgggcg aggagcggac cggcgtgcgg aacggggtcg
     1561 ccgccgaggg tccggccggg ctggtggacg gaccgctcgc cgacggcaag ctgacgatgg
     1621 ccgagtggcg ggaggtcctg ttcaagacgg cctcctcgcg ccacatccgg gagttcgagg
     1681 acggcaacgt ctgcgcgacc gccgcgcagt tcaacacgta ccccgtcaag tacgggcaga
     1741 tcccgaagca ggcgcccgcc tggctgatcg tcggctacgg cgcgatcgat ggcccgtcac
     1801 ggcggctcgc gtactccgtg ctcaacgggg agcgcgcgat gccggtccgg acccaggaag
     1861 acgcctactt caccgtcgac gatgcggcac ggcgcgcgac ccacgaggtc tacagccggc
     1921 cctgaggaag gaaggaggaa gtccatgcga cgacgactgc tcgcaccgat cgcgatgatc
     1981 ggcgtccttg ctctcgccgc ccccgccacc ggggttccgg tggtggcgat ctccatcccc
     2041 gaggacggag cgacgatctc ccgtagcacg ggttcggcgc tccaggtcgg aggggccgtg
     2101 gagttcgcgg tccccgagaa gggatcgcgg cggttctacg tccggcggac ctcgtgcaac
     2161 gccgatcagc gcctgagcat cacccggggg agcgagaccg cgggctgtgc tcagaccgtt
     2221 tcgttgaccc ccgtcgcgga ggaaacggcg acggcggtga cgtacgccgc ggagaacggg
     2281 gttccgttca cgctggacgc gacccagccg atcaccggga ccctctcgct cgtcagctac
     2341 ctcctggtga cggggaacgt cggcgtcgga gccggtctcg tcaccgccga catcagcgtg
     2401 acgggcgccg aggtgggcgg agccgagcag gcgctcggct cgacgaccat ctcgtacacc
     2461 gccacgcccg cgacctaccg ggtcgacacg ccgtggagca tcgcgccgcc ggcgacgctc
     2521 gacaagaagg acttcctggg cctgacgctt tcgatcaccg tccgcggccg gaacgcgctg
     2581 cacggcttca cccggcccaa cgacacgaac ttcctcgtgc cgatctggac ggcgagcttc
     2641 aaccgtcgcg tcgaggttgc gatcgacaac gggagcttca cgtcgacggg gatctcggtc
     2701 gggcccgacc tcacgacctg gtccggcagc cggccgtggc cgtcgctcgg gacccacgtg
     2761 ctccgcgccc gggccgtcca gggcggggtc gtctccggca tcgaccagcg cgccttcacg
     2821 atcacgtcct aggtccgacg gtcgacgagg ggccgccctg ctcgccgggc ggcccctccc
     2881 cttctccagg ctcaggcgta ctgcgcgccc gcccctgcct gcgccggctc gtgatgcccc
     2941 tcggcgatct cctcgacgag tttgcggcag aaggcgggaa ggtcggcggg cttccggctg
     3001 ctgacgagcc cgttgtccac gtggacctct tcgtccaccc aggtgccacc cgcgttctcg
     3061 atgtcggttc ggacgctggg gtacgaggtc agggtccggc cgcggacgac gcctgcctcg
     3121 acgagcgtcc acgggccgtg gcagatcacg gccaccggcc ggccgctgcc gacgaactcg
     3181 cggacgaacg agacgctggc ctcgtccatc cggagcagat ccgggttctt cacgcccccc
     3241 ggtaggatca ggccgtcgta ctcatccggc ctcgcgtcgg cgaccgcccg gtctacctcg
     3301 aacgcggccc ccttctccat gccgttcatc gcctggatcg catccggctt cggagagagg
     3361 agctccaccg tggccccggc gtccatcacc gcctgccacg gctccgtgag ctcgacctgc
     3421 tccaccccat ccgtcgccag gaaggcgacc cgcttgccct gcagctcgtt ggccatctcg
     3481 aacctccgtt ctcggtgatg acgtcgttcc ctcggcccct acccggaggg tcgatggcgg
     3541 taaccgaggg gagcgtgatt ttgggcggat gcacaaagga agggcccggg tgcgggtcga
     3601 accctccgcg gacaccgaac agaaggggga tccatgcgca ggctcgccgc cgccaccatc
     3661 acgaccgtcc tcgccgtctg cctcctcgcg cccccggccg ccgcgtaccc acgcccgggg
     3721 aaaaccctgc gggtgaaccc cctgctcgac ctgacgggcg ggccggcgag cggcggcacc
     3781 ccgcagctct ccggcgacgg ccgccacgtc ctcttcccga ccaccgcccg gctggtcgaa
     3841 gatgacaccg gcctcgcggt cgactgctac gtccgtgacc tggtgacctg gtccctggtc
     3901 cgcgcgtcgg tcgcgcacga cgggaccgag gcgaacggtt cctgctcctc gcctgcgatc
     3961 tcccgcgacg gccgctacgt cgcgtggcac agcaccgcca cgaacctcgt ccccggggac
     4021 acgaacgggt tggctgacat cttcgtgcgc gacctggtcg ccgggacgac cacgcgcgcg
     4081 tcggtgtcgg cacagggcgc gcaggcggac ggcgcctcga cgagcgccgc gatctccgac
     4141 gacggtcgcg tcgtcgcctt cctctcgagc gccacgaacc tcgtggcggg cgactcgaac
     4201 gcgttcaacg acgtcttcgt gcggaacatg gaggacggga gcatccagat cgcgacgctg
     4261 tcgaccgagg gcacgcaggg tgtcaccgcc gtctcgcagt tcgagctcag cggcaacggc
     4321 cgccacgtcg tcttcgtcca ccggatgccg tgggagggtg acaagagcag ctacgac
//
LOCUS       DATLGG010000102         3478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_115074, whole genome shotgun sequence.
ACCESSION   DATLGG010000102 DATLGG010000000
VERSION     DATLGG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115074"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VM638_02845"
     CDS             <1..408
                     /locus_tag="VM638_02845"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVM11402.1"
                     /translation="VEGAADSGSLITAELAQDLGRDVAAVPGPATSPVSAAPLELIRD
                     GGALVRGPADVLELVGVRGDRQDLAAMSLSRREQQVLSGVSGPPTTLDAVAAAIGRPP
                     AEVLAALASLEIKGFVRADAGRYQRTAAAATAG"
     gene            505..1404
                     /locus_tag="VM638_02850"
     CDS             505..1404
                     /locus_tag="VM638_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HVM11403.1"
                     /translation="MASRGGEPLIERFIEHLALERRLSAHTVAAYRTDLHGLATFLER
                     AGVSLEEATYPHLRRWLAHLVTRGYARSSLARKAASVRSFYRYLLRRGLVAADPAALL
                     RGPARAHTLPNVLRAPEAARLVEAPSDVDPWALRDRAILELLYGAGIRVAELCTLDVD
                     DVEAGEGRVRVMGKGSKERQAPIGQPAAEALTAYIRDVRPTLVRPESGPALFVNRRGR
                     RIGPREIRRMVGEYGAQVLPGRRTTPHTLRHSYATHLMEGGADIRAVQELLGHASLAT
                     TQRYTHVSRRRLFGAYRDSHPRA"
     gene            1405..2211
                     /gene="whiG"
                     /locus_tag="VM638_02855"
     CDS             1405..2211
                     /gene="whiG"
                     /locus_tag="VM638_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HVM11404.1"
                     /translation="MARRQTSEDRPRPDDIEASELDRLWSEFKDVGSPVARERLILHY
                     APLVKYVVGRLAAGLPQTVERADLTSYGMFGLMDALDKFDRGREVKFETYAIPRIKGA
                     IIDELRAMDWVPRSIRFKAREVEKAHSGLEAKLRRAPTDAEVAEHLGITLSELHDVVS
                     QISFVSVMALDEMVGGGDDAGEKRSLLDTLADANTTDPTAEIEGQEMRGLLAAAINDL
                     SEREKIVITLYYFEGLTLAEIGEILGVTESRVCQIHTKAVGGLRNNLADD"
     gene            2270..2842
                     /locus_tag="VM638_02860"
     CDS             2270..2842
                     /locus_tag="VM638_02860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVM11405.1"
                     /translation="MGSSVVTLALGFAVLAVVGLDARPATAPEGSAARAAVEVPAPGT
                     YAWPVEGAVLRPFELPSSTYGPGHRGIDIGTPAGTRVLAAERGLVAFAGVVAGERYVS
                     VDHPDGVRTTYSWLASIAVRRGDRIERGAILGTTSRGHPGSETPHLHLGARFRGVYVD
                     PLLLLEPRGVAGLIHLAPLDPGSPLGPPRP"
     gene            2854..>3478
                     /locus_tag="VM638_02865"
     CDS             2854..>3478
                     /locus_tag="VM638_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM11406.1"
                     /translation="MPRSLRFRLRHALLLSVLALLGAGAAGPALGAQTPDAWPQVGGD
                     PSHAATASTPGPPYRRAWTTQIAGGGAAAGPVVAGETIVVVGWDRVVALRLSDGGQTW
                     SVEREPGPAGPPAVVEEQVIHAEGLGRLGAVVALSLEDGSEVWRHDVGTAIVGGITVV
                     PDGAIVGGRDGSIRLLDPETGDERWARDLPGRIETTPTSDGRAIFVLT"
BASE COUNT          459 a         1264 c         1294 g          461 t
ORIGIN      
        1 gtcgagggcg ccgccgacag cggctccctc atcacggccg agctcgccca agacctgggg
       61 cgcgacgtgg cggccgttcc cggtccggcc acgagcccgg tatccgcagc gccgctcgag
      121 ctgatccgcg acggcggggc cctcgtgcgc ggtcccgcgg atgtcctcga gctggtcggg
      181 gttcgggggg atcggcagga tctggcggcg atgtcgctgt cccgccgcga gcagcaggtc
      241 ctgtccggcg tgagcgggcc gccgacgacc ctggacgccg tcgccgccgc catcgggagg
      301 ccacccgccg aggtgctggc cgccctcgcc tcgctcgaga tcaagggctt cgtccgggcc
      361 gacgcgggtc ggtaccagcg gaccgccgcc gccgcgacgg ccggatgacg cgccgcgggg
      421 cgaggaggcc ttccgggcgc ggatgtcctt cgtccggcgc acgcgctgtg ctacaaacgg
      481 gtggcccccc agggaaagga cccgatggca tcgcgtggcg gggaacccct gatcgaacgc
      541 ttcatcgagc acctcgcgct ggagcggcgc ctctccgccc acacggtggc ggcgtaccgg
      601 accgacctcc acgggctggc gacgttcctc gagcgagcgg gggtgagcct cgaggaggcg
      661 acgtacccgc acctccgccg ttggctcgcg cacctcgtca cccgcggata cgcgcgctcc
      721 tcgctcgcgc ggaaggcggc gtcggtgcgg tcgttctacc ggtacctgct gcggcggggg
      781 ctcgttgccg ccgaccccgc cgccctcctc cgcgggccgg cgcgtgcgca cacgctgccc
      841 aacgtcctgc gcgctcccga ggctgcccgg ctcgtcgaag ccccctctga cgtcgacccg
      901 tgggccctgc gcgaccgagc gatcctcgag ctcctctacg gcgcgggcat ccgcgtcgcg
      961 gagctctgca cgctcgacgt cgacgatgtg gaggcggggg agggtcgcgt gcgggtgatg
     1021 gggaaggggt cgaaggagcg gcaggctccg atcggccaac ccgccgcgga ggcgctgacg
     1081 gcctacatcc gcgacgtccg cccgaccttg gtacggcccg agagcggccc ggcgctgttc
     1141 gtgaaccggc ggggacggcg catcgggccg cgcgagatcc gcaggatggt gggagaatac
     1201 ggggcgcagg tgctgccggg gcgccggacg acgccgcaca ccctgcggca ctcgtacgcg
     1261 acccacctca tggagggagg cgcagacatc cgagccgttc aggagctcct gggacacgcg
     1321 agcctcgcga cgacgcagcg gtacacgcac gtctcgcgcc ggcggctctt cggcgcctac
     1381 cgcgacagcc acccgagggc gtagatggcg aggcgacaga cgtccgagga ccggccccgc
     1441 cccgacgaca tcgaggcgtc ggagctcgac cggctctggt cggagttcaa ggacgtcggc
     1501 tcgcccgtcg cccgggagcg gctcatcctc cactacgcgc cgctcgtcaa gtacgtcgtc
     1561 gggcgcctcg ccgcggggct cccgcagacg gtcgagcggg cggacctgac gtcgtacggc
     1621 atgttcgggc tcatggatgc gctcgacaag ttcgaccgcg gccgcgaggt gaagttcgag
     1681 acctacgcga tcccgcggat caagggggcg atcatcgatg agctccgggc gatggactgg
     1741 gtgccccgct cgatccggtt caaggcccgc gaggtcgaga aggcgcactc ggggctcgag
     1801 gcgaagctgc gccgggcgcc gaccgacgcc gaggttgcgg agcacctcgg catcaccctc
     1861 tcggagctcc acgacgtggt gagccagatc tcattcgtct cggtgatggc gctcgacgag
     1921 atggtcggcg gcggcgacga cgccggcgag aaacgcagcc tgctcgacac gctcgcggac
     1981 gccaacacca ccgacccgac ggccgagatc gagggacagg agatgcgcgg gctgctcgcg
     2041 gccgcgatca acgatctgtc ggagcgcgag aagatcgtca tcacgctcta ctacttcgaa
     2101 ggactcaccc tcgccgagat cggcgagatc ctcggggtga ccgaatctcg cgtctgccag
     2161 atccacacga aggccgtcgg tgggctgcgg aacaacctcg ccgacgactg agactgtcag
     2221 acccccgcgc taacatccgc cccctccccc gcgacgcagg aggatcggga tggggagctc
     2281 ggtcgtgacc ctcgcgctcg gcttcgcggt actggcggtc gtcgggttgg atgcgcgtcc
     2341 agcgacggcg ccggaagggt cggcggcacg agcggcggtc gaggtgccgg cgccggggac
     2401 gtacgcctgg cccgtcgagg gcgcggtcct acggccgttc gagctcccga gctcgacgta
     2461 cgggccggga caccggggga tcgacatcgg cacgcccgcc ggcacccgcg tcctcgccgc
     2521 cgagcgagga ctcgtggcgt tcgcgggtgt cgtcgccggg gagcgctacg tatccgtcga
     2581 tcaccccgac ggcgtccgga ccacgtactc gtggctcgcg tcgatcgccg tccgccgggg
     2641 cgaccgcatc gagcgcgggg cgatcctcgg caccacctcg cgagggcacc cgggcagcga
     2701 gacgccgcat ctccacctcg gcgcccggtt ccgcggcgtc tacgtcgacc cgctcctgct
     2761 cctcgagccg cgcggcgtcg ccgggttgat ccacctggcg ccgctcgacc ccggttcccc
     2821 cctcggtccg ccgcgaccct agaatgggcg accgtgcctc ggtccctccg cttccggctc
     2881 cgccacgccc tgctcctcag cgtgctcgcc ctgctcgggg cgggcgcggc cggccccgcg
     2941 ctcggcgctc agacgccgga cgcctggccg caggtcggcg gcgatccctc gcacgccgca
     3001 accgcctcca cgccgggacc gccctaccgg cgcgcgtgga cgacccagat cgccggtggt
     3061 ggcgcggccg cgggtcccgt cgtggccggg gagacgatcg tggtcgtcgg ttgggaccgg
     3121 gtcgtcgcgc tccgtctgag cgacggcggg cagacgtgga gcgtggagcg cgagccgggg
     3181 ccggcggggc cgccggccgt cgtcgaggag caggtgatcc acgccgaggg gctcggtcgg
     3241 ctcggtgccg tggtcgcgct ctcgctcgag gacgggagcg aggtctggcg acacgacgtc
     3301 gggacggcga tcgtcggggg gatcacggtc gtgcccgacg gtgcgatcgt cgggggtcgc
     3361 gacggctcga tccggctgct ggacccggaa acgggcgacg agcggtgggc gcgggacctt
     3421 ccggggcgga tcgagacgac cccgacctcc gatgggcggg cgatcttcgt cctgactc
//
LOCUS       DATLGG010000103         4747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_54327, whole genome shotgun sequence.
ACCESSION   DATLGG010000103 DATLGG010000000
VERSION     DATLGG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54327"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VM638_02870"
     CDS             <1..643
                     /locus_tag="VM638_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVM11407.1"
                     /translation="ARTEWISRLTTAVPGVEDMLGRTIATIIENRTGAGLIGAAGLVW
                     TGMGVVEASRFALGRTYRTGPRPGFLKRKGAVLANLLALGPLALAGVVGTSLIAGIDA
                     GTPRGFALETLGWVLVAAAGVALFMVSFRLLIRRPGPPWRELWPGAVLAAAGWVGLQL
                     FGAFYITRVVTRATAVYGSFAAVVGLLILLSLAARLFLFAATLNAVLMERDPG"
     gene            complement(640..2088)
                     /locus_tag="VM638_02875"
     CDS             complement(640..2088)
                     /locus_tag="VM638_02875"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM11408.1"
                     /translation="MARKLFIAGAWTEGSSDETIDVRSPVTGEILATVPVASERDVDE
                     AVRAARAGQEALEIMTVFERAELLHRAAELMTSRKDELGRELTLEQGKPLHTEGIAEI
                     EESAENFRIAAEDVKRMTTEVLPSEDRNKKMFTFRKANGVYAVITPWNFPFVIPSELL
                     APALAGGNACVLKPSELTPLIAARMIEILEEAGFPPGSVSLVNGGSDVGRALVTHPGV
                     DAIAFVGSHETGEAIVRAAGLKRTLMELSGNGPVIVLDDANVEHAADMAVFGAQYVAG
                     QCCVATERVLVSERVHDRFLERAVAAARDVVLGDPFAETTTMGPLNNEDVATKTDRHL
                     AEARDRGVRVLAGGGRAEGFPTSLYYELTVADGVPTDSLLFREETFGPVVPVTTFATD
                     EDALRLANDSHLGLQAAVFTSSLKRAFTFIDGLRTGNVVVNDTTDYWEALQPFGGASG
                     TRTGWGRVGGKYAIEDMTDLRTAVVDFRNTAD"
     gene            2069..3334
                     /locus_tag="VM638_02880"
     CDS             2069..3334
                     /locus_tag="VM638_02880"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVM11409.1"
                     /translation="MKSLRAIAPQPSLARSPAGPAERRIRRCPVVRGVASGPVETAAI
                     LRSMRIAILGAGAMGSAAARLAARHEADLTIADPDAARAEALAQATGATAVAFDGSRK
                     RLVEILRDADAVAACIPFRLNLEVMEAALEARCPYADLGGLYHMTLRQLELDERFRRA
                     GVPAILGIGMAPGITNLLARLGADRLETVESVDLVNGSVDPGGFGVPYSPETILDEFA
                     EPAIVFEEGEVREVPPASGGIRWTLPEPMGEMEAVYTLHSELATLPRTIEGVRAVRWR
                     LALPPDVERGFRTLVDLGLASTEPVDVTSGQVVPRELLLAVLTRLPQPEGPPADAEAT
                     HVRVEGTSEGRPATFLGEVVCRPTAEGIGAGAFGTALPIAVAARWLADGRVGPGVHPP
                     ETALDPEAFLQDLARDGLEISLSLQERLV"
     gene            3370..3443
                     /locus_tag="VM638_02885"
     tRNA            3370..3443
                     /locus_tag="VM638_02885"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3404..3406,aa:His,seq:gtg)
     gene            3484..3557
                     /locus_tag="VM638_02890"
     tRNA            3484..3557
                     /locus_tag="VM638_02890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3518..3520,aa:Lys,seq:ctt)
     gene            complement(3601..3771)
                     /locus_tag="VM638_02895"
     CDS             complement(3601..3771)
                     /locus_tag="VM638_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11410.1"
                     /translation="MQRAERIELEVRRPVGAGILRGMLTRIRDRRQERRELAAWFRDN
                     PPEIGRSTGCRV"
     gene            3888..4427
                     /locus_tag="VM638_02900"
     CDS             3888..4427
                     /locus_tag="VM638_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM11411.1"
                     /translation="MAVARRHFAERGYAGTATEDLVKEAGVTRGALYHHFRDKADLFR
                     AFVQEGEVELLRRVGQAADAAGADPWERLQAGAQGFLDGCLEPAVQRCLLEAPSVLGW
                     EEWREIRTRSTLGLLTGAIERAIEAGTITPQPVEPLAQMLYGALYEAGLAIARAPDRT
                     EARREIGESVALLFRALRR"
     gene            complement(4429..>4747)
                     /locus_tag="VM638_02905"
     CDS             complement(4429..>4747)
                     /locus_tag="VM638_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HVM11412.1"
                     /translation="QVHVCWAEDAEEAKRTAYEWWPNTALPGNLFVELESPKHFEAVT
                     ELISQDDVAGSIPTGPDPEPYIEAIREYAEAGYDHVYLHQIGPDQQRFLEFAEREILP
                     ALS"
BASE COUNT          613 a         1718 c         1696 g          720 t
ORIGIN      
        1 cgcgaggacc gagtggatct cgcgcctcac gaccgccgtc ccgggcgtcg aggacatgct
       61 cgggcggacg atcgccacga tcatcgagaa ccgcacgggc gcgggcctca tcggcgcggc
      121 cgggctcgtc tggacgggga tgggggtcgt cgaggcgtcg cggttcgcgc tcggccgcac
      181 ctaccggacg gggccgcggc cgggcttcct gaaacggaag ggggccgtgc tggcgaacct
      241 cctcgccctc gggccgctcg cgctcgctgg ggtcgtcggg acctcgctga tcgccgggat
      301 cgatgcgggg acccctcggg gcttcgccct cgagacgctc ggctgggtgc tcgtcgcggc
      361 cgcgggcgtg gcgctgttca tggtgtcgtt ccgcctgctg atccgccgac ccgggccgcc
      421 gtggcgggag ctctggcccg gcgccgtgct cgccgccgcc ggatgggtcg ggctccagct
      481 cttcggagcc ttctacatca cgcgcgtcgt cacccgggcg acggccgtct acggatcgtt
      541 cgcggcggtg gtcggcctgc tgatcctgtt gagcctcgcg gcgcggctct tcctgttcgc
      601 ggcgacgctc aacgcggtgc tgatggaacg cgacccgggc tagtcggccg tgttgcggaa
      661 gtccacgacc gccgtgcgga ggtcggtcat gtcctcgatc gcgtacttcc cgccgacgcg
      721 cccccacccg gtgcgcgttc ccgacgcgcc gccgaacggc tggagcgcct cccagtagtc
      781 ggtcgtgtcg ttcaccacga cgttcccggt ccgcaggccg tcgatgaacg tgaacgcgcg
      841 cttgaggctc gaggtgaaga ccgcggcctg gagcccgagg tgcgagtcgt tcgcgagccg
      901 gagcgcgtcc tcgtccgtcg cgaaggtcgt caccgggacc accgggccga acgtctcttc
      961 ccggaacagg agcgagtccg tggggacgcc gtcggcgacc gtcagctcgt agtacagcga
     1021 ggtcgggaag ccctcggccc tcccgccgcc ggcgaggacc cggacgcccc ggtcgcgtgc
     1081 ctccgcgagg tggcgatccg tcttcgtcgc gacgtcctcg ttgttgagcg ggcccatcgt
     1141 ggtcgtctcg gcgaaggggt ccccgagcac gacgtcgcgc gcggcggcga cggcccgctc
     1201 gaggaagcgg tcgtgcacgc gctcggacac gaggacccgt tcggtcgcga cgcagcactg
     1261 gccggcgacg tactgcgcgc cgaacaccgc catgtcggcg gcatgctcga cgttcgcgtc
     1321 gtcgaggacg atgaccggtc cgttcccgga gagctccatc agggtccgct tgaggccggc
     1381 cgcgcgcacg atcgcctcgc ccgtctcatg cgacccgacg aaggcgatcg catcgacgcc
     1441 ggggtgggtc acgagcgccc ggccgacgtc cgagccgccg ttgacgagcg acaccgaccc
     1501 cggcgggaag ccggcctcct cgaggatctc gatcatcctc gccgcgatca gcggcgtcag
     1561 ctccgacggc ttcaggacgc aggcgttgcc gccggcgagc gccggggcca ggagctccga
     1621 ggggatgacg aacgggaagt tccacggcgt gatgaccgcg tacacaccgt tcgccttgcg
     1681 gaacgtgaac atcttcttgt tgcggtcctc cgaggggagg acctccgtcg tcatgcgctt
     1741 cacgtcctcg gccgcgatcc ggaagttctc ggccgactcc tcgatctcgg cgatcccctc
     1801 ggtgtgcagc ggcttcccct gctcgagcgt cagctcccgc ccgagctcgt ccttgcggga
     1861 cgtcatcagc tcggctgccc ggtggaggag ctcggcgcgc tcgaagacgg tcatgatctc
     1921 gagcgcctcc tggccggcgc gcgcggcccg gacggcctca tcgacatcgc gctcggaggc
     1981 gacgggcacc gtcgcaagga tctcccctgt caccgggctg cgcacgtcga tcgtctcgtc
     2041 ggatgagccc tcggtccagg ccccggcgat gaagagcttg cgcgccatcg cgccccagcc
     2101 tagcctggcg cgctcgcctg ccgggcccgc cgaacggcgc atccggcgat gccccgttgt
     2161 acgcggcgtc gcgagcggac cggtggagac cgcggctatc ctccgctcga tgcgcatcgc
     2221 gatcctcgga gccggcgcca tgggcagcgc cgccgcgcgc ctcgccgcgc ggcacgaggc
     2281 cgacctcacg atcgccgacc ccgatgcggc acgcgccgaa gccctggctc aggcgaccgg
     2341 ggccacggcc gtcgccttcg acggctcccg aaagcgcctc gtcgagatcc tccgcgatgc
     2401 ggacgccgtc gcggcctgca tccccttccg cctcaacctc gaggtgatgg aggcggcgct
     2461 ggaggctcgc tgtccgtacg cggacctcgg cggcctgtac cacatgacgc tccgccagct
     2521 cgagctcgac gagcggttcc gacgggcggg cgtgccggcg atccttggga tcgggatggc
     2581 gccggggatc acgaacctgc tcgcgcgcct cggtgcggat cgcctcgaga cggtggagag
     2641 cgtcgacctc gtgaacggct cggtcgaccc cggcggcttc ggcgtcccct actcccccga
     2701 gacgatcctc gatgagttcg ccgagccggc gatcgtgttc gaggagggcg aggtacggga
     2761 ggtgccgccg gcctcaggag ggatccgctg gaccctgccc gagccgatgg gcgagatgga
     2821 ggccgtctac acgctccact ccgagcttgc cacgctcccg cgcacgatcg agggcgtccg
     2881 cgcggtccgc tggcggctcg ccttgccgcc ggacgtcgag cgcggcttcc ggacgctcgt
     2941 cgacctcggc ctcgcctcga ccgagccggt ggatgtgacg tcgggccagg tcgtgccgcg
     3001 agagctcctg ctggcggtcc tcacccggct gccccagccg gagggtccgc ccgccgatgc
     3061 ggaggcgacg cacgtgcgcg tcgaggggac gtctgagggg cgaccggcca ccttcctcgg
     3121 agaggtggtc tgccgcccga ccgccgaggg gatcggcgcc ggagccttcg ggacggctct
     3181 gccgatcgcc gtcgcggccc gctggctcgc cgacggccgc gtcgggcccg gggtccaccc
     3241 cccggagacg gccctggacc cggaggcctt cctgcaggac ctggcgcggg acggcctcga
     3301 gatatcgctg tcgctccaag agcggctcgt ctgagcgacc cgtcgccgct gctaccatgt
     3361 gcccgcatgg tggccgtagc tcaatcggtt agagcgccgg gttgtggtcc cggaggctgg
     3421 gggttcgagt cccctcggtc accctcctaa cctgcgaggg gcctgtatcc tctcggagtt
     3481 gacgggccgt tagctcaatc tggcagagca ggggactctt aatcccaagg ttgtaggttc
     3541 aagtcctaca cggcccacgt acccgcctcc tgacgaggcc gttgcccgag cggggaaccc
     3601 tcagacgcgg cagccggtgg agcggccgat ctccggcggg ttgtcgcgga accaggcggc
     3661 cagctctcgc cgctcctgcc gccggtcccg gatccgggtg agcatgcctc gcaggatccc
     3721 cgctccgacc ggccgccgta cctccagctc gatccgctcg gcccgctgca tccgtgcctc
     3781 ctctcccccg acggggtatc atacagacgg tacgtatcct acatacagac agtatggatg
     3841 tcaaagggag ccaagcggca cgctcggagg ctacgcggga ggccctgatg gccgtcgccc
     3901 gccgccactt cgccgagcgg ggctacgcgg gcaccgccac ggaagacctg gtcaaggagg
     3961 ccggggtcac ccggggcgcc ctctaccacc acttccgcga caaggccgac ctcttccgcg
     4021 cgttcgtcca ggagggggag gtcgagctgc tccgccgggt cgggcaggcg gccgacgcgg
     4081 cgggagcgga tccatgggag cggctgcagg cgggcgcgca gggcttcctc gacggctgtc
     4141 tcgagccggc ggttcaacgc tgcctgctcg aggcgccatc cgtcctcggc tgggaggagt
     4201 ggcgcgagat ccggacccgc agcacgctcg gcctgctgac cggcgcgatc gagcgggcga
     4261 tcgaggcggg cacgatcacg ccacaaccgg tcgagccgct ggcccagatg ctctacgggg
     4321 cgctctacga ggccggcctg gcgatcgcgc gcgcgccgga ccggaccgag gcacggcgtg
     4381 agatcggcga gagcgtcgcg ctgctcttcc gggccctccg tcgctgagct aggagagcgc
     4441 cggcaggatc tcccgctcgg cgaactccag gaaccgctgc tggtcggggc cgatctggtg
     4501 caggtacacg tggtcgtacc cggcctccgc gtactcccgg atcgcctcga tgtagggctc
     4561 cggatcgggc ccggtcggga tcgaacccgc gacgtcgtcc tgcgagatca gctcggtcac
     4621 ggcctcgaag tgcttcgggc tctcgagctc gacgaagagg ttgccaggca gcgcggtgtt
     4681 cggccaccac tcgtatgcgg tgcgcttcgc ctcttctgcg tcctcggccc agcagacgtg
     4741 gacctgg
//
LOCUS       DATLGG010000104         5835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_86310, whole genome shotgun sequence.
ACCESSION   DATLGG010000104 DATLGG010000000
VERSION     DATLGG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86310"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..812)
                     /gene="rplA"
                     /locus_tag="VM638_02910"
     CDS             complement(102..812)
                     /gene="rplA"
                     /locus_tag="VM638_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HVM11413.1"
                     /translation="MKRGKRFNDAMKAFNRDDLYTPVDAIRILKSLPTAKFDETVEVA
                     LRLGVDPRKADQIVRGTVSLPNGTGRTVRVAAFAVGDKAREATEAGADVVGGEDLVEQ
                     VTKGNIDFDAAVATPDMMPVVGRAGRVLGPRGLMPNPKAGTVTNDIGKAVADIKGGKV
                     EYRTDRQGNVHVIVGKRSFDETALAENLHAILDEVVRAKPAAAKGRYIKSVTMSTTMG
                     PGIRIDPAKVRDVEVANA"
     gene            complement(809..1246)
                     /gene="rplK"
                     /locus_tag="VM638_02915"
     CDS             complement(809..1246)
                     /gene="rplK"
                     /locus_tag="VM638_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HVM11414.1"
                     /translation="MAAPRRKKVLALVKLQIKAGQATPAPPVGTALGQHGVNIMEFCK
                     AYNEATQAQMGQVIPVELTIYEDRSFTFVTKQPPAAELLKQAAGVEKGSGEPNRDKVA
                     SVSRDQVRQIAETKMKDLNANDVDAAMKIIEGTARSMGIEVAS"
     gene            complement(1308..2231)
                     /gene="nusG"
                     /locus_tag="VM638_02920"
     CDS             complement(1308..2231)
                     /gene="nusG"
                     /locus_tag="VM638_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HVM11415.1"
                     /translation="MTDQDIRDAAQDEPAGDEDETPVPTPATMADLEDQDPPVPETLE
                     DAAGAAGTGGPEGDAASDSEPGDEPTLVEAQEEDEPEAPAAEAPEPVSDVEDVPPPAA
                     DDDDAEEPEQAEEGSAQPETTDSDDPFSGPGDWYVIHCYAGYENKVKSNLGTRIQSMQ
                     MQDKIFDVVVPMEDVMEIKGGRKQVVQKKFLPGYVLVKMIYDNDSWFVVRNTPGVTGF
                     VASGTGQKPTPLSRREIEKILVVKKEEQKPAFRLEFEEGDVVRIISGPFADFNGTIDE
                     INMDQSKVKVMVNIFDRETPVELSFDQVARV"
     gene            complement(2242..2526)
                     /gene="secE"
                     /locus_tag="VM638_02925"
     CDS             complement(2242..2526)
                     /gene="secE"
                     /locus_tag="VM638_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HVM11416.1"
                     /translation="MNRQAKRQMQRGRQQAGDRAAAARRAVPDRKRERTSPRQFFREV
                     RQELKKVNWPTKRELGTYTVVVLFTVVVLTSLVFGIDFLVGRAVLAVFGS"
     gene            complement(2543..2616)
                     /locus_tag="VM638_02930"
     tRNA            complement(2543..2616)
                     /locus_tag="VM638_02930"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2580..2582),aa:Trp,seq:cca)
     gene            2825..3988
                     /locus_tag="VM638_02935"
     CDS             2825..3988
                     /locus_tag="VM638_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11417.1"
                     /translation="MRVPDSGDDVDIHAVLAAQQAAVAARLEEGLQDIQRRADQLMRR
                     VGAEVFRAMQQGGGAPLKDDAARSILTYVDERFQTLNMAVGRLEQAVQKLAQATGDVL
                     LRTDKSREAVDRVAIAAAEQSRRTQQGMTQLATRVERSIQPVAARLDAVEAAVKAATA
                     RQRADLAAFTERTSEGLVRVGQRLREGFAAVAKRSDERTRAVGEEIAASVRAEIERLE
                     EALAEQAERSATAAERVALAAQESTARVERALESLGQRLDARLEDAVTETEALVNEAV
                     TRMARRQTKLLEDAIAEVQDRRPPEAPVPLHPVAAEPPGAPSEEEPPPVAVGIGPEPV
                     FEEPEPEPDPESEAHGDEVQETVAPIDGELDHDLDQLRSVLAELADFDGPLSD"
     gene            complement(3985..4236)
                     /locus_tag="VM638_02940"
     CDS             complement(3985..4236)
                     /locus_tag="VM638_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11418.1"
                     /translation="MTGYDDQPLIEALRALREEPVEPPPGFEERLEEHLRRELRWRVP
                     ARRIAHDRRVRYGASVGGVVVAVAAIALVLRRQSKRAAA"
     gene            complement(4233..4763)
                     /locus_tag="VM638_02945"
     CDS             complement(4233..4763)
                     /locus_tag="VM638_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM11419.1"
                     /translation="MVTPELLQACRRGERGAFEELVERTRRQVYSMARRLMRDAHEAE
                     DVAQEVYLRVYRGLPTFRQEASFETWLYRIVANTAFTQMRRRGRFGDVAPEPMGDEAP
                     SRDRPLGEAAMDRDDLTRALAKLPAASRAVVVLRDVYGLTMKEIASEMGTSEGAVKVR
                     LHRARTRLRELLAEDR"
     gene            complement(4812..5234)
                     /locus_tag="VM638_02950"
     CDS             complement(4812..5234)
                     /locus_tag="VM638_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HVM11420.1"
                     /translation="MSLRFEDVNVGDELPERSNRVDRAQLVMYAGASGDFNPIHWNEE
                     FARMVGLPGVISHGMFTMALVTRALTDWLGDAEPIRKVSVQFRKEVRPDETVVARGRV
                     EEKDPDTRTVRVSLWAELEREGETIKPIKNGEAIVELP"
     gene            complement(5231..5683)
                     /locus_tag="VM638_02955"
     CDS             complement(5231..5683)
                     /locus_tag="VM638_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HVM11421.1"
                     /translation="MPLNSSLIGKEYPGVTYEVGREKIREFAAAVGETAAIFHDPEAA
                     KQAGHPEQVAPPTFPTVLHLKSGPQVIADPELGLNYALVVHGTQEFEYRRPVYAGDIL
                     TATPRIADIQAKGPHEFLTIETTMTAAGGEVVCIARATLISRGTAASQ"
     gene            complement(5685..5825)
                     /locus_tag="VM638_02960"
     CDS             complement(5685..5825)
                     /locus_tag="VM638_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVM11422.1"
                     /translation="MALMLIALGYYGRPEDVANAHLFLASEEADYISGVILPVAGGQL
                     GV"
BASE COUNT          788 a         2130 c         1981 g          936 t
ORIGIN      
        1 tcgcgtgcct cggcgggtgc ccccggaggg gattacgctt cctcgccccc gctgtctgcg
       61 gcccgcccag catacgggat gccggccggg ggtgtcgacc gctacgcgtt cgcgacctcg
      121 acgtcgcgga ccttcgccgg gtcgatccgg atccccgggc ccatcgtcgt cgacatcgtg
      181 acgctcttga tgtagcggcc cttcgcggcg gccggcttcg cccgcacgac ctcgtcgagg
      241 atcgcgtgca ggttctccgc gagcgcggtc tcgtcgaacg agcgcttccc gacgatcacg
      301 tggacgttcc cctgccggtc ggtgcggtac tcgaccttgc cgcccttgat gtcggcgacg
      361 gccttgccga tgtcgttcgt caccgtcccg gccttgggat tcggcatcag cccgcggggg
      421 ccgaggaccc gcccggcgcg cccgacgacc ggcatcatgt ccggcgtcgc gaccgcggcg
      481 tcgaagtcga tgttgccctt cgtgacctgc tcgacgaggt cctcgccacc gacgacgtca
      541 gcccccgcct cggtcgcctc ccgagccttg tccccgacgg cgaaggccgc gacacgcacg
      601 gtgcggccgg tgccgttggg caggctgacg gtcccgcgga cgatctggtc cgccttccgc
      661 gggtcgaccc cgagccggag cgcgacctcc accgtctcgt cgaacttcgc ggtgggcagg
      721 ctcttgagga tccggatcgc gtcgacgggc gtgtagaggt cgtcacggtt gaacgccttc
      781 atcgcgtcgt tgaaccgctt gccccgcttc atgacgccac ctcgatgccc atgctgcggg
      841 cggtcccctc gatgatcttc atcgccgcgt cgacgtcgtt cgcgttgagg tctttcatct
      901 tcgtctccgc gatctgccgg acctggtcgc ggctgacgct cgccaccttg tcgcggttcg
      961 gctcgcccga ccccttctcg acccccgcgg cctgcttcag aagctccgcc gccgggggct
     1021 gcttcgtgac gaacgtgaag ctccggtcct cgtagatcgt gagctcgacc gggatgacct
     1081 ggcccatctg ggcctgggtg gcctcgttgt aggccttgca gaactccatg atgttgacgc
     1141 cgtgctgccc gagcgccgtc cccaccgggg gtgcgggcgt ggcctgaccg gccttgatct
     1201 ggagcttcac gagcgccagg accttcttgc gcctgggtgc tgccatctcc gtcctcctcc
     1261 gcggtccgaa cggcggttcc ccgcctcccg cgtccgatct tctcgcgcta gacccgcgcc
     1321 acctggtcga agctcagctc gacgggggtc tcccggtcga agatgttgac catcaccttc
     1381 accttggact ggtccatgtt gatctcgtcg atcgtcccgt tgaagtccgc gaacggcccg
     1441 gagatgatcc ggacgacgtc gccctcctcg aactcgaggc ggaaggcggg cttctgctcc
     1501 tccttcttga cgacgaggat cttctcgatc tcccggcgcg agagcggggt cggcttctga
     1561 ccggtcccgg aggccacgaa gcccgtcaca ccgggggtgt tgcgaacgac gaaccacgag
     1621 tcgttgtcgt agatcatctt cacgagcacg tacccgggaa ggaacttctt ctggacgacc
     1681 tgcttgcggc cgcccttgat ctccatcacg tcctccatcg ggacgaccac gtcgaagatc
     1741 ttgtcctgca tctgcatcga ctggatccgc gtgccgaggt tgctcttcac cttgttctcg
     1801 tagcccgcgt agcagtggat cacgtaccag tcgcccggcc cggagaacgg gtcgtcgctg
     1861 tcggtcgtct cgggctgcgc ggacccctcc tcggcttgct ccggctcttc ggcatcgtcg
     1921 tcgtccgccg cgggcggagg cacgtcctcc acgtcggaga ccggctcggg agcctccgcc
     1981 gcgggcgcct ccggctcgtc ctcctcctga gcttcgacca gcgtcggctc gtcaccgggc
     2041 tcgctgtcgg acgccgcgtc gccctcggga ccaccggtcc cggccgctcc ggcagcgtcc
     2101 tccaacgtct ccggcacggg agggtcctga tcctcgaggt cggccatcgt cgcgggggtg
     2161 gggacgggcg tctcgtcctc gtctccggcg ggctcgtcct gagcagcgtc gcggatgtcc
     2221 tggtcggtca tatggggatc ctcaggagcc gaagacggcg agcaccgcgc gtcccacgag
     2281 gaagtcgatg ccgaagacca gcgaggtcag aacgacgacc gtgaacagga cgacgacggt
     2341 gtaggtgccg agctcccgct tcgtcggcca gttcaccttc ttcagctcct gccggacctc
     2401 ccggaagaac tggcgggggc tcgtgcgctc gcgcttgcga tccgggacgg cgcgccgcgc
     2461 cgcggcggcg cggtcgccgg cctgctgccg ccctcgctgc atctgcctct tggcttgcct
     2521 gttcatcacg ttccttccgc ggcagggccg gagggactcg aacccccaac cgccggtttt
     2581 ggagaccggt gctctcccaa attgagctac ggccctattc aggggcttgg tacggagcgg
     2641 gacccgccca gtatacagag ccccgcggaa cccgtcgccc caggtgcgtg aaccgggccc
     2701 cgacgtagcc tacactcgag tcggaactcg cgcggccccc cggagcgagg accagatgca
     2761 ggagatacca gagcgacccg aggcaggggc cgcctcccga cgtcgcctcc gccgtccttc
     2821 gcccgtgcgc gtccccgact ccggggacga cgtcgacatc cacgccgtcc tcgccgctca
     2881 gcaggctgcg gtcgccgcgc ggctcgagga ggggctgcag gacatccagc gccgggccga
     2941 tcagctgatg cggcgggtcg gcgccgaggt cttccgcgcg atgcagcagg gtggcggagc
     3001 gccgctcaag gatgacgcgg cgaggagcat cctcacctac gtcgacgagc gcttccagac
     3061 gctgaacatg gccgtcggcc gcctcgagca ggcggtccag aagctcgcgc aggcgaccgg
     3121 cgacgtcctc ctgcgcaccg acaagagccg cgaggcggtc gaccgcgtgg cgatcgcggc
     3181 agccgagcag tcgcggcgga cccagcaggg catgacgcag ctcgccacca gggtggagcg
     3241 ctcgatccag ccggtcgcgg cccgcctcga cgcggtcgag gccgccgtga aggccgcgac
     3301 cgcccgtcaa cgcgcggacc tcgcggcgtt caccgagcgc acgagcgagg ggctggtgcg
     3361 ggtcgggcag cggctccgcg aggggttcgc cgccgtcgcc aagcggtccg acgagcggac
     3421 gcgggcggtc ggcgaggaga tcgccgcctc cgtccgggcc gagatcgagc gcctcgagga
     3481 agcgctcgcc gagcaggccg agcgcagcgc cacggccgcc gagcgggtcg cgcttgccgc
     3541 gcaggaaagc accgcacggg tcgagcgcgc gctggagtcg ctcggccagc gcctggacgc
     3601 gaggctcgag gacgcggtga ccgagacgga ggcgctcgtc aacgaggccg tcacgcggat
     3661 ggcgcgccgt cagacgaagc tgctcgagga cgcgatcgcc gaggtgcagg atcggcggcc
     3721 cccggaggcg ccggtgccgc tgcacccggt ggcggccgag ccccccgggg cgccctcaga
     3781 ggaggagccc ccgccggtgg cggtggggat cggcccggag ccggtcttcg aggagcccga
     3841 gcctgagccc gaccctgagt ccgaggcgca cggcgacgag gtccaggaga cggtcgcgcc
     3901 gatcgacggg gagctcgatc acgacctcga tcagcttcga tcggtgcttg ccgagctcgc
     3961 ggacttcgac ggaccgctct cggactaggc cgcagcccgc ttcgactgcc tccgcaggac
     4021 cagcgcgatc gcggcgaccg cgacgacgac cccaccgacc gaagcgccgt atcggacgcg
     4081 gcggtcgtgg gcgatccgac gggccggcac ccgccagcgc agctctcgcc ggagatgctc
     4141 ctcgaggcgc tcttcgaaac ccggcggcgg ttcgacgggc tcctcacgca gcgcgcggag
     4201 cgcttcgatg agcggctggt cgtcgtaccc ggtcatcgat cctccgcgag gagctcgcgg
     4261 aggcgggtgc gggcgcggtg gagccgcacc ttcacggccc cttcggaggt gcccatctcg
     4321 ctcgcgatct ccttcatcgt caggccgtac acgtcgcgca ggacgacgac tgcgcgcgat
     4381 gccgcgggaa gctttgcgag cgcgcgggtc agatcgtcgc ggtccatggc ggcctccccg
     4441 agggggcggt cccgcgaggg cgcctcgtcc cccatcggct cgggggccac gtcgccgaag
     4501 cggcctcggc gccgcatctg cgtgaaggct gtgttcgcga cgatgcggta cagccaggtc
     4561 tcgaagctcg cctcttgacg gaacgtcgga aggccccggt acacgcggag gtagacctcc
     4621 tgcgccacgt cctcggcctc gtgcgcatct cgcatcagac gtcgtgccat cgaatacacc
     4681 tgtcgtcgcg tccgctcgac cagctcctcg aaggcgcccc gctcgccgcg gcggcaggct
     4741 tggaggagct cgggcgtcac catgacgccc gctcgttcga ccggggagcg gcgacggagg
     4801 ttacgtccct cctagggcag ctcgacgatc gcctcgccgt tcttgatcgg cttgatcgtc
     4861 tcgccctccc gctcgagctc cgcccagagc gagacgcgaa cggtgcgcgt atcggggtcc
     4921 ttctcctcca cccggccccg cgccacgacc gtctcgtcgg gacggacctc cttgcggaac
     4981 tgcaccgaga ccttccggat cggctcggcg tcgccgagcc agtccgtcag cgcgcgcgtg
     5041 acgagcgcca tcgtgaacat gccgtgcgag atcacccccg gaaggccgac catgcgcgcg
     5101 aactcctcgt tccagtggat cgggttgaag tcgcccgatg cgcccgcgta catcacgagc
     5161 tgcgctcgat cgacacggtt cgaccgctcc ggcagctcat cgccgacgtt cacgtcctcg
     5221 aagcgcaggc tcactggctc gccgccgtcc cgcgggagat gagcgtggcg cgggcgatgc
     5281 acacgacctc tcccccagcc gctgtcatgg tcgtctcgat cgtgaggaac tcgtgcggcc
     5341 ccttcgcctg gatgtcggcg atccgcggcg tcgcggtgag gatatcgccg gcgtagacgg
     5401 gccgccggta ctcgaactcc tgcgtcccgt gcaccacgag cgcgtagttg agcccgagct
     5461 ccggatcggc gatcacctgc gggcccgact tcaggtgcag gaccgtcggg aaggtcggtg
     5521 gcgcgacctg ctccggatgt cccgcctgct tcgcggcctc cgggtcgtgg aagatcgcgg
     5581 ccgtctcccc caccgccgcg gcgaactctc ggatcttctc gcggccgacc tcgtaggtga
     5641 cgcccgggta ttccttcccg atcagggagc tgttcagcgg cacctcagac ccccagctgt
     5701 ccgccggcaa ccgggaggat cacaccggag atgtagtcgg cctcctcgct cgcgaggaac
     5761 aggtgcgcgt tcgccacgtc ctccggccgg ccgtagtagc cgagcgcgat cagcatcaag
     5821 gccatcgccc ggacc
//
LOCUS       DATLGG010000105        10068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_225582, whole genome shotgun sequence.
ACCESSION   DATLGG010000105 DATLGG010000000
VERSION     DATLGG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225582"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /gene="murD"
                     /locus_tag="VM638_02965"
     CDS             complement(<1..187)
                     /gene="murD"
                     /locus_tag="VM638_02965"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVM11423.1"
                     /translation="MTLAERRALVLGLAESGVAAAEALLERGAHVRVSEARPPEEVAE
                     AAARVEGLGAEVLAGGHD"
     gene            complement(184..1248)
                     /gene="mraY"
                     /locus_tag="VM638_02970"
     CDS             complement(184..1248)
                     /gene="mraY"
                     /locus_tag="VM638_02970"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HVM11424.1"
                     /translation="MTSILVSAALGLFLTLLGTPIAIRIFRAFGWGQRVREPGPHYPQ
                     HMQKMGTPTMGGLVILGATTVAYLATTLTLGPFTRTAVLVLGAALGFGLVGFIDDLMK
                     IRRADRHSLGIGKAAKLAGQGIVAVLFALGLTRLTDASTELSFIRGTGIDLGTIAFVI
                     WVFVILASTSNAVNLTDGLDGLASGSGALVMAAFVIISFWQFRHTCGALTGPGCYEVD
                     INTALDLAIVSAAAMGAIAGFLWWNAAPARIFMGDTGSLAIGGLIGAMAVATETQLLL
                     LILGGLYVLETTSVILQVVSFRLFKRRLFRMAPIHHHFEVLGWHEFTVIVRFWIIAGL
                     AVAFGLGLFYADFLARGGIS"
     gene            complement(1245..2618)
                     /gene="murF"
                     /locus_tag="VM638_02975"
     CDS             complement(1245..2618)
                     /gene="murF"
                     /locus_tag="VM638_02975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HVM11425.1"
                     /translation="MRERTLREIAADLDGVIRGADAPVTSVATDTRELRPGSLFVALR
                     GARVDGHDLMDEAARAGAAGALVERSVEAPIPTVEVPDTGAALLELAARERDALATPL
                     VAITGSVGKTSVKDLTAAVLGARFRVTASPRSFNTEVGVPLTILAADERTEAIVCEMG
                     SRGIGHISLLCDVAKPDVGVVTAVGAAHLEMFGSLDVVRDAKAELPEALSEGGTAVLN
                     ADDPVVRGFVDRTRARPLLYGRSEGADVRAVELSLDPEGVPSFTLVAGGERERVELAV
                     PGEHMASNALAAAACGIALGLSPAECAAALKDATVSPWRMERRITPEGVRVLNDAYNA
                     NPMSMAAALRSAVWMKGEGRCIAVLGAMAELGPTAAAEHERVGELVARLGVDRLVVVG
                     DDARMIGIGAVREGVEPDRVRYCRDPEEAAATVRAEARAGDVVLVKGSRAAGLERVAE
                     RLWGDAP"
     gene            complement(2615..4063)
                     /locus_tag="VM638_02980"
     CDS             complement(2615..4063)
                     /locus_tag="VM638_02980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HVM11426.1"
                     /translation="MTPPRTLRELIDGLPAAEVVGEAVVPVRDVVYASGDVAPGALFF
                     CVPGSRRDGHDFAAGAVASGAVALVTERRLDIDGATQVIVPSVREAMGPIAAAFHGHP
                     AARLTLVGVTGTNGKTTTTYVLEQVFRAAGMTPGVIGTTGVRVDGHPRPLARTTPEAP
                     DLHRLLAGMAEEGVEGVAMEVSSHGLDQHRVGGARFSCAVFTNLSQDHLDYHGTLEAY
                     FEAKAKLFTPAMAAHAAVNHDSPEGRRLLGRGLPTMTYGIGEGADVRATDVRLPPDGI
                     AFRVDGLEVRSELRGHYNVHNCLAALTAARQVGIDDAASVRGIAAVPVVPGRLEPVRV
                     GQPFQVLVDYAHTPDSVENVLRAARRLTQGRVIVVLGCGGDRDRSKRPLMGRAATSLA
                     DLSVITSDNPRSEDPASIIAEIEPGAREGGGRYLLEVDRRAAIRLALGEARSGDVVVI
                     AGKGHETGQEFADRTIPFDDRVVAAEELERLR"
     gene            complement(4111..5835)
                     /locus_tag="VM638_02985"
     CDS             complement(4111..5835)
                     /locus_tag="VM638_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVM11427.1"
                     /translation="MSAPPPPRPPAGRLASLFLVLVLALVAVAGRLFQLQLGDAPAYE
                     ALARDQRIRTIDLPASRGAIYDREGAELALSMPARAVYARPALVPDPASAARRLARAL
                     GIDERTILRALRSGRSFVYLARRVDLEAAARAERLDLPGIGFLDESRRSYPGATLGSQ
                     VLGFVGVDGQGLAGLELSHEKLLAGRPGEMIVEQDPLGNPIPQGERRVEPPVPGQDLV
                     LTLDQDLQFRAQRALKRAVRRNGAKGGTVIVMEPASGEILAMASYPWFDPNDVGSAPP
                     ENLRARAVTDIYEPGSVNKVIVAAAALEEEIVGLEEVFDVADHLRVGPKVFKDAHPHP
                     PMEMTLSDIVAWSSNVGSIQVAQRVGKPTLARYLRRFGFGRPTGIRFPGEGAGILPPV
                     EEWWETGMGTIPIGQGVAVTPLQIATVYATIANGGVWVQPKLVRATVDAEGRVRETGA
                     PERRRVISAGTARAVTSMLAYAVSDGTGTGAQIPGYWVAGKTGTARKPLEGVRAYSDE
                     YMASFVGFVPAEDPRLVVAAILDEPDTVYGGIAAAPMFRDVARFALAQLRVPTSDPPA
                     RPPTARAP"
     gene            complement(5832..6296)
                     /locus_tag="VM638_02990"
     CDS             complement(5832..6296)
                     /locus_tag="VM638_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11428.1"
                     /translation="MAVAADRLRPPSHRARRRVPTGRRAAGRRAAARTRARVATRARR
                     IPFGVTALCLVALVILGLVAAQALVTQDSFRLAELARRAEALEADYGRLRLEAARLSS
                     PDRIASAARRAGMVLPEQVELIRLPAVAPAGSSEETTELGTFALKGVLGGGP"
     gene            complement(6296..7159)
                     /gene="rsmH"
                     /locus_tag="VM638_02995"
     CDS             complement(6296..7159)
                     /gene="rsmH"
                     /locus_tag="VM638_02995"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HVM11429.1"
                     /translation="MAEAPHEPVLVAEVVRLMSGASVVLDLTVGAGGHAEALLRAGVG
                     RVVGVDRDPDALALAAGRLGSTGFTPVAGRFSDVERVASEAGVEGVDGVLYDLGVSSM
                     QLDRAERGFSYRRPGPLDMRMGDEGLTAADVVNGYPEEELARVIRELGEERFAGRIAA
                     AIVRARERAPLASTEELAAIVAGAVPRRRGGGHPARRTFQAIRLEVNRELEELADSLP
                     RSVSLLAPGGRVVVISYHSLEDRIVKRAFLADDRLEVLTRKPVVAGSRERARNPRARS
                     AKLRAAERRAA"
     gene            complement(7265..7693)
                     /gene="mraZ"
                     /locus_tag="VM638_03000"
     CDS             complement(7265..7693)
                     /gene="mraZ"
                     /locus_tag="VM638_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HVM11430.1"
                     /translation="MAELLGEHHYQLDPKGRLSLPAKFREAFADGVYLTLGQDGCVFA
                     FPREEWEQRKAEVAGLPISDPRNRAYSRMFFGSAERVELDAQGRLVLPRHLRETARLG
                     REVAVVGVSDRLEIWDGGEWGRYREDHGGSYTSGALVPER"
     gene            complement(7864..8346)
                     /locus_tag="VM638_03005"
     CDS             complement(7864..8346)
                     /locus_tag="VM638_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVM11431.1"
                     /translation="MGRALVLNASFEPLAVVPARRAVVLVLKDKAEMLETNGVIFRSE
                     RLELPAPSVVRLSYFVKVPYRARASLTRRAVFARDEWTCQYCGGTAENVDHVIPRSRG
                     GAHVWENVVAACRPCNSRKENRLPEEVGTPLRRAPFAPRDGFRLLLGRLEPEWEPYLT
                     "
     gene            8637..9590
                     /locus_tag="VM638_03010"
     CDS             8637..9590
                     /locus_tag="VM638_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM11432.1"
                     /translation="MSLDRREFQGVFGAIRDNVERVVQGKPREVELALVSLVAEGHLL
                     IEDVPGVGKTMLAKAIARSIDCSFRRIQFTPDLLPSDITGVNVYSPERGDFEFKPGAV
                     FANIVLGDEINRASPKTQSALLESMEERQATVDGVSHQLAVPFMVLATQNPIEYEGTY
                     PLPEAQLDRFMMRLSIGYPSPEAEAEILATHGDGTRLDEIQPVADAVAVARLIRMAAG
                     VHAAEPLRRYVVDLADATRRHPDLHLGASPRASLMLLRAARALAASRGRDFVTPDDVK
                     HLAVQVMAHRLIPTGEAAMTGRTPAAVVGDLLQEVPVPVSG"
     gene            9590..>10068
                     /locus_tag="VM638_03015"
     CDS             9590..>10068
                     /locus_tag="VM638_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11433.1"
                     /translation="MLTPRGLGLIAGSAALWLGSRMLAGPDLHLVAVGLIGLPAFALI
                     SLARPSRPLQVSRRLSSTRVFPGGHIEVSLELRGDGLRGPVLCEDALPSGSGRGARAV
                     VVGEGAARRMRYTLTCRTRGRYRVGPLTVFEADPFDLARRRREVAGTHEFVVFPEVE"
BASE COUNT         1443 a         3736 c         3619 g         1270 t
ORIGIN      
        1 ggtcgtggcc gccggcgagg acctcggcgc cgagcccctc gacgcgagca gccgcctcgg
       61 cgacctcctc gggcggccgc gcctcgctca cgcgcacgtg cgcaccgcgc tcgaggaggg
      121 cctcggctgc cgcgacgccg ctctcggcga gccccagcac gagcgctcgg cgttccgcga
      181 gcgtcacgag atccctccgc gtgcgaggaa atcggcgtag aagaggccga ggccgaaggc
      241 caccgcgagc ccggcgatga tccagaaccg cacgatcacg gtgaactcgt gccagcccag
      301 gacctcgaag tgatggtgga tcggcgccat ccggaagagc cgccgcttga acagccggaa
      361 cgagaccacc tggaggatca ccgacgtcgt ctcgaggacg tagaggccgc cgaggatcag
      421 caggaggagc tgcgtctcgg tggccacggc catcgcgccg atcaggccac cgatcgccag
      481 ggagcccgtg tcccccatga agatgcgcgc cggcgcggcg ttccaccaca ggaacccggc
      541 gatcgcgccc atcgccgccg cggagacgat cgcgaggtcg agcgccgtgt tgatgtcgac
      601 ctcgtagcac ccgggcccgg tcagcgcgcc gcaggtgtgg cggaactgcc agaacgagat
      661 gatgacgaac gcggccatca ccagggcccc ggaacccgac gcgagcccat cgagcccgtc
      721 ggtgaggttc accgcgttcg acgtcgaagc gaggatcacg aagacccaga tcacgaaggc
      781 gatcgtcccg aggtcgatcc cggtgccgcg gatgaacgac agctcggtgg acgcgtcggt
      841 cagccgcgtg agtccgagcg cgaagaggac ggcgacgatc ccctgtcccg cgagcttcgc
      901 tgccttcccg atgccgagcg agtgacgatc ggcccggcgg atcttcatca ggtcgtcgat
      961 gaacccgacg aggccgaagc cgagggcggc gccgagcacg aggaccgccg tccgcgtgaa
     1021 aggcccgagc gtgagggtcg tcgcgaggta ggcgaccgtg gtggcgccga ggatcacgag
     1081 gccgcccatc gtgggcgtcc ccatcttctg catgtgctgg gggtagtggg gccccggctc
     1141 gcgcacgcgc tgcccccagc cgaaggcgcg gaagatccgg atcgcgatcg gcgtgccgag
     1201 aagcgtcagg aacagcccga gcgccgccga gacgaggatg ctcgtcacgg ggcgtccccc
     1261 cacaggcgct cggcgacccg ctcgagcccc gcggcgcgcg agcccttcac gagcacgacg
     1321 tctccggcac gcgcttccgc ccgcacggtc gcggcggcct cctccggatc cctgcagtag
     1381 cggacccggt ccggctcgac gccttcgcgc acggcgccga tcccgatcat ccgtgcgtcg
     1441 tcgccgacga cgaccagacg atcgacgccg agccgcgcga cgagctcccc cacccgttcg
     1501 tgctcggccg ccgcggtggg cccgagctcc gccatcgcgc ccagcaccgc gatgcagcgc
     1561 ccctctccct tcatccagac ggccgagcgg agggccgccg ccatcgacat cgggttggcg
     1621 ttgtacgcgt cgttcaggac gcggaccccc tcgggcgtga tccgtcgctc catccgccag
     1681 ggcgagacgg tcgcgtcctt cagggccgcc gcgcattccg ccggcgacag gcccagcgcg
     1741 atgccgcacg ccgccgcggc gagcgcgttc gacgccatgt gctcaccggg gaccgcaagc
     1801 tcgacccgtt cgcgctcgcc gcccgcgacg agggtgaacg acgggacgcc ttccggatcg
     1861 agcgagagct cgacggctcg cacgtccgcg ccctcggagc ggccgtacag gagcggccgg
     1921 gcgcgggtcc ggtccacgaa cccccggacg acggggtcgt ccgcgttcag gaccgcggtc
     1981 cccccttcgg acagcgcctc cggaagctcc gccttcgcgt cacggacgac gtcgagcgag
     2041 ccgaacatct cgaggtgcgc ggcgcccacg gccgtgacga cgccgacgtc gggcttcgcg
     2101 acgtcgcaga ggaggctgat gtgaccgatg ccccggctgc ccatctcgca gacgatcgcc
     2161 tcggtccgct cgtcggccgc gaggatcgtg aggggcaccc cgacctcggt gttgaacgag
     2221 cgcgggctcg ccgtgacccg gaaccgggcg ccgaggaccg cggcggtgag gtccttcacc
     2281 gaggtcttcc cgaccgaccc ggtgatcgcg acgagcggcg tcgccagggc atcgcgctcg
     2341 cgcgccgcca gctcgaggag cgcggccccg gtgtcgggga cctccacggt cgggatcgga
     2401 gcctcgacgc tccgctcgac cagcgccccg gccgctccgg cccgagctgc ctcgtccatg
     2461 aggtcgtgac cgtcgacgcg cgcgccgcgg agcgcgacga acaggctgcc cggccgaagc
     2521 tcacgcgtgt ccgtcgcgac gctcgtgacg ggcgcgtcgg cgccacggat gacgccgtcg
     2581 aggtccgccg cgatctcgcg aagggtccgc tccctcatcg cagccgctcc agctcctcgg
     2641 cggccaccac gcgatcgtcg aacgggatcg tccgatcggc gaactcctgc ccggtctcgt
     2701 gccccttgcc cgcgatgacc acgacgtcgc ccgaacgagc ctcgccgagc gccaggcgga
     2761 tcgcggcgcg gcggtcgacc tcgagcaggt agcggcctcc cccctcgcgt gcccccggct
     2821 cgatctccgc gatgatcgat gcgggatcct cggatcgggg gttgtcgctc gtgatcacgc
     2881 tgaggtcggc cagcgaggtc gccgcgcgcc ccatgagcgg ccgcttcgag cggtcgcggt
     2941 cgccgccgca gccgagcacg acgatcaccc ggccctgggt gagccgccgc gcggcgcgca
     3001 gcacgttctc gacgctgtcg ggggtgtgcg cgtagtcgac gaggacctgg aagggctggc
     3061 cgacgcgcac cggctcgagc cggccgggca cgaccggcac cgccgcgatc ccgcggacgc
     3121 tcgcggcgtc gtcgatcccc acctgccggg cagccgtgag cgccgcgagg cagttgtgca
     3181 cgttgtaatg accgcgcagc tccgaacgga cctcgagccc gtcgacgcgg aacgcgatcc
     3241 cgtccggcgg cagccgcacg tccgtcgccc ggacgtcggc accctcgccg atgccgtacg
     3301 tcatcgtcgg cagtccacgg cccagcagtc gccggccctc ggggctgtcg tggttgaccg
     3361 ccgcatgcgc tgccatcgcg ggggtgaaga gcttggcctt cgcctcgaag taggcctcga
     3421 gggtcccgtg gtagtcgagg tggtcctgcg agaggttcgt gaacacggca caggagaagc
     3481 gtgctccgcc cacccggtgc tggtcgagcc cgtgcgacga cacctccatc gccacgccct
     3541 ccacgccctc ctccgccatg ccggcgagga gccggtggag atcgggagcc tccggcgtgg
     3601 tgcgcgcgag cgggcgcggg tgaccgtcga cgcgcacccc tgtcgtgccg atcacgcccg
     3661 gggtcatccc cgcggcgcgg aagacctgct cgagcacgta ggtcgtcgtc gtcttcccgt
     3721 tcgtcccggt gacacccacg agggtcagcc gggccgcagg gtgcccgtgg aaggcggcgg
     3781 cgatgggccc catcgcttcg cggacggagg gaacgatcac ctgcgtggct ccgtcgatgt
     3841 cgagacgtcg ctccgtgacg agggcgaccg ctccggacgc caccgcaccc gcggcgaagt
     3901 cgtggccgtc ccgccgcgag ccggggacgc agaagaagag ggcgccgggc gcgacgtcgc
     3961 cggacgcgta caccacgtcg cgcaccggga cgaccgcctc gcccaccacc tcggccgcag
     4021 gcaggccgtc gatcagctcg cggagcgtgc gggggggagt caacgaacct cgatccggga
     4081 acgggggacg ccgtgattct accagcggcc tcatggcgcc cgagccgtcg gcggacgcgc
     4141 cggcgggtcc gaggtcggga cgcgcagctg cgcgagggcg aagcgggcga cgtcgcggaa
     4201 catcggcgct gcggcgatgc ctccgtagac ggtgtcgggc tcgtcgagga tcgcggcgac
     4261 gaccaggcgc gggtcctcgg ccggcacgaa gccgacgaac gacgccatgt attcgtcgct
     4321 gtacgcgcgg acaccctcga ggggcttccg cgcggttccc gtcttccccg cgacccaata
     4381 gccggggatc tgcgcgcccg tccccgtccc gtcggagacg gcgtaggcga gcatggatgt
     4441 gaccgcacga gccgtgccgg cggagatgac ccggcggcgc tccggagcgc cggtctcgcg
     4501 cacgcgtcct tcggcgtcca ccgtggcgcg cacgagcttg ggctggaccc acaccccccc
     4561 gttcgcgatc gtcgcgtaca ccgtcgcgat ctgcagcggc gtcaccgcca cgccctggcc
     4621 gatcgggatc gtgcccatcc cggtctccca ccactcctcg accggcggga ggatgccggc
     4681 gccctcgccg gggaaccgga tcccggtcgg ccgaccgaac ccgaagcggc gaaggtagcg
     4741 cgccagcgtg ggcttgccga cccgctgcgc gacctggatc gacccgacgt tgctcgacca
     4801 cgcgacgatg tccgagagcg tcatctccat cggcggatgc ggatgcgcgt ccttgaagac
     4861 cttcgggccg acgcggaggt gatcggccac gtcgaagacc tcctcgagac cgacgatctc
     4921 ctcctcgagc gccgcggccg ccacgatcac cttgttcact gaaccgggct cgtagatgtc
     4981 cgtcaccgcg cgtgcccgca ggttctccgg cggcgcggag ccgacgtcgt tcgggtcgaa
     5041 ccagggatag gaggccatcg cgaggatctc gccggacgcc ggctccatca cgatcacggt
     5101 gccgcccttc gccccgttcc ggcggaccgc gcgcttcagc gctcgctggg cccggaactg
     5161 gaggtcctgg tcgagcgtga ggacgagatc ctgcccgggc acggggggct ccacgcgacg
     5221 ctccccctgc gggatcgggt tcccgagggg gtcctgctcg acgatcatct cgccgggccg
     5281 gcccgcgagg agcttctcgt ggctgagctc gaggcccgcg agaccctggc catccacacc
     5341 cacgaacccg agcacctgcg agccgagcgt cgcgcccggg tacgagcgcc gactctcgtc
     5401 gaggaagccg atccccggga ggtcgagacg ctccgcgcgc gccgccgcct cgaggtccac
     5461 gcgccgggcg aggtagacga agctccgccc tgaccgcagc gcccgcagga tcgtccgctc
     5521 gtcgatcccg agcgcccgcg ccaggcgtcg ggcggccgac gcagggtcgg ggacgagcgc
     5581 cgggcgcgcg tagacggcgc gcgcgggcat cgacagcgcg agctcggcgc cttcccgatc
     5641 gtagatcgcg ccgcgtgacg ccggcaggtc gatcgttcgg atccgctggt cgcgcgcgag
     5701 cgcctcgtac gcgggagcgt ccccgagctg gagctggaac agccgccccg ccaccgcgac
     5761 gagggccagg acgaggacga ggaacaacga tgcgagacgg ccggcgggtg gccgcggcgg
     5821 cggcggggcg ctcacggccc gcctccgagg acccccttca aggcgaacgt ccccagctcg
     5881 gtagtctcct cggatgagcc ggcgggagcg acggcgggga gccggatcag ctcgacctgc
     5941 tccggcagga ccatgcccgc gcgtcgggcg gccgacgcga tccggtccgg cgaggagagc
     6001 cgggccgcct cgagccggag cctgccgtag tccgcctcga gggcctcggc ccgccgggcg
     6061 agctcggcga ggcggaacga atcctgcgtc acgagggcct gggcggcgac gagcccgagg
     6121 atgacgagcg ccaccaggca gagggcggtg accccgaacg ggatgcgacg cgcacgcgtc
     6181 gcgaccctcg cacgggtccg agcggccgcg cggcggccgg cggcccgacg cccggtgggg
     6241 acgcggcggc gcgctcggtg cgacggcggg cggaggcggt ccgcggcgac ggccatcagg
     6301 cggccctccg ctccgcggcg cgcagcttcg cgctgcgggc acgggggttc cgagcccgct
     6361 cgcgggagcc ggcgacgacc ggcttgcgcg tcagcacctc gagccggtcg tcagccagga
     6421 acgcgcgctt cacgatgcgg tcctcgagcg agtggtagga gatgaccacg acccgcccgc
     6481 cgggggcgag gaggctcacc gaccggggaa gggagtcggc gagctcctcg agctcccggt
     6541 tcacctccag gcggatcgcc tggaaggtcc tgcgcgcggg gtgcccgccg ccgcggcgcc
     6601 gcgggaccgc gcccgcgacg atcgcggcga gctcctcggt gctcgccagc ggcgcacgct
     6661 cgcgtgcacg aacgatcgcg gccgcgatcc ggccggcgaa ccgctcctcc ccgagctcgc
     6721 ggatgacccg cgcgagctcc tcctcggggt agccgttcac gacgtccgcg gcggtcaagc
     6781 cttcgtcgcc catccgcatg tcgaggggtc cgggtcggcg gtaggagaac ccgcgctcgg
     6841 cgcggtccag ttgcatcgac gaaacgccca ggtcgtagag gacgccgtcc acgccctcga
     6901 cgccggcctc cgacgccacg cgctcgacgt ccgagaagcg cccggcgacc ggggtgaagc
     6961 cggtggaccc gaggcgcccc gcggcgagcg cgagcgcgtc ggggtcccgg tccacgccga
     7021 ccacccgccc cacgccggct ctcagcagcg cctcggcgtg accgccggcg ccgaccgtca
     7081 ggtcgagcac cacgctcgcc ccggacatca ggcggaccac ctccgcgacc aggaccggct
     7141 catgcggcgc ctcagccaag cacccggatc gcgagcagcc cgaggaccac gtaggtcccg
     7201 accgcgccgg ccacgagcgc cagcaggtac agcgtctgtc cgacctctcg ctgcatcgcc
     7261 cctcctatcg ctcggggacg agcgcgcccg aggtgtagga accgccgtgg tcctcgcggt
     7321 accgccccca ctcgccgccg tcccagatct cgaggcggtc ggagaccccg accaccgcaa
     7381 cctcacgccc gaggcgcgcc gtctcccgga ggtgccgcgg cagcaccagc cggccctgcg
     7441 cgtcgagctc gacccgctcg gcgctgccga agaacatccg ggagtaggcg cggttccgcg
     7501 gatccgagat cggcaggccc gccacctcgg ccttccgctg ctcccactcc tcgcgtggga
     7561 aggcgaagac gcagccgtcc tgaccgaggg tgaggtagac gccgtcggcg aaggcctcgc
     7621 ggaacttcgc cggcagcgag agccgtccct tcgggtccag ctggtagtgg tgctcaccga
     7681 gcagctcggc cacgcgtcct cccctgtact ccaccgcgtg ccacgtttat gccacccgca
     7741 ctccaccggg tcaaggagat cgcgtgaatt acagtggtgt aagtcagtac ttgtgaactc
     7801 gtgttgcaac atgagcaaca agagcaacac ggcgggaagg gaggggcgat cagccgcggc
     7861 cgatcaggtg aggtagggct cccactcggg ctcgaggcga ccgaggagga gccggaagcc
     7921 gtcgcggggg gcgaacggcg cgcggcgcag gggggtcccc acctcctcgg ggaggcggtt
     7981 ctccttgcgc gagttgcagg ggcggcaggc cgcgacgacg ttctcccaca cgtgggcccc
     8041 cccgcgggag cgagggatca cgtggtcgac gttctcggcg gtgcccccgc agtactggca
     8101 ggtccactcg tcacgggcga acacggcgcg gcgcgtgagg gaggccctgg cgcggtacgg
     8161 gaccttcacg aagtagctga gccgaacgac ggatggcgcc ggcagctcga ggcgctcgga
     8221 tcggaagatg acgccgttcg tctcgagcat ctccgccttg tccttcagga cgagcacgac
     8281 cgcccgccga gccggcacga ccgcgagcgg ctcgaacgat gcgttcagga ccagcgctct
     8341 tcccaactca tcaccccgaa ggcgggcccg gcttgcgcca ttgtaccgga gcgcttcagc
     8401 ggaagggctc gatcgaccga tcggatgctt gactccggcg actcacacgc gtgtaattcg
     8461 ggtggcgggc cggtgcgccg cgagcgcggg cgcccgaacc gcaggggccg cgcggggcgt
     8521 tcgaggcttg cgacggcgcc cggcccgcca tccactcccc ctttcccgcg gtctcgcgcg
     8581 tacactggtc ctccgaccag ccgaccgcgc cccgcgcagg agccccggtg cagcagatgt
     8641 cgctcgaccg acgcgagttc caaggtgtct tcggggcgat ccgcgacaac gtcgagcggg
     8701 tcgtccaggg gaagccgcgc gaggttgagc tggcgctcgt cagcctcgtg gcggaggggc
     8761 acctgctgat cgaggacgtg cccggtgtcg gcaagacgat gctcgcgaag gcgatcgctc
     8821 gctcgatcga ctgctcgttc cgccggatcc agttcacgcc cgacctcctg ccgagcgaca
     8881 tcaccggcgt gaacgtctac agccccgagc gcggagactt cgagttcaag cccggcgccg
     8941 tgttcgcgaa catcgtcctc ggcgacgaga tcaaccgtgc cagcccgaag acgcagtcgg
     9001 ccctgctcga gtcgatggag gagcgccagg cgacggtcga cggcgtcagc caccagctgg
     9061 ccgtgccgtt catggtcctc gcgacgcaga acccgatcga gtacgagggg acctaccccc
     9121 tgccggaggc gcagctcgac cgcttcatga tgcgtctgtc gatcggctac ccctcccccg
     9181 aggccgaggc ggagatcctc gcgacccacg gggatggaac gcggctcgac gagatccagc
     9241 cggtcgccga cgcggtcgcc gtcgcgaggc tgatccggat ggcggcgggg gtgcacgccg
     9301 cggagccgct gcgacgctac gtagtcgatc tggccgacgc gacgcgacgc cacccggacc
     9361 tccatctcgg cgcgagcccg cgcgcatcgc tcatgctcct gcgtgcggcg cgcgcgctcg
     9421 ccgcctcgcg cggccgcgac ttcgtcaccc ccgacgatgt gaagcacctc gcggtccagg
     9481 tcatggccca tcggttgatc ccgacgggcg aagccgcgat gacggggcgg acaccagccg
     9541 cggtcgtcgg cgacctcctg caggaggtgc cggtgccggt gagcggctga tgctcacccc
     9601 gaggggcctg ggactgatcg ccggatcggc cgcgctgtgg ctcggctcgc ggatgctcgc
     9661 gggcccggat ctgcacctcg tggcggtggg actcatcggg ctgccggcgt tcgccctcat
     9721 ctcgctcgca cggccgagcc ggccgctcca ggtgagccgg cgcctgtcga gcacccgcgt
     9781 gttccccggg ggccacatcg aggtcagcct cgagctgcgc ggcgacggcc tccgcgggcc
     9841 ggtcctctgc gaggacgccc tcccgtcggg gtccgggcgc ggagcgcgcg cggtcgtcgt
     9901 cggcgaggga gcggcgcggc ggatgcgcta cacgctcacg tgtcggacgc gaggccggta
     9961 ccgggtgggg cccctcacgg tcttcgaggc cgatcccttc gacctcgcgc gccgccgacg
    10021 agaggtggcg ggcacccacg agttcgtcgt gttcccggag gtcgagga
//
LOCUS       DATLGG010000106         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_210029, whole genome shotgun sequence.
ACCESSION   DATLGG010000106 DATLGG010000000
VERSION     DATLGG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210029"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VM638_03020"
     CDS             complement(<1..641)
                     /locus_tag="VM638_03020"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HVM11434.1"
                     /translation="MRPPSIRLRRRPPADPGSLSAPLARVIRSGVVESVHLGSVAVAD
                     AAGGLLAWSGDPSGDVFARSCMKPLQAAVSLSFVEEALSEEEVAVMCASHNAETIHLD
                     AVEALLARGGRRAGDLRCPPAWPMDPARAREVEAPESRYHDCSGKHAGMLLACSRAEM
                     DPAGYLDRDHPLHRAVMRAIPDAAGAEPSAIGTDGCGVPVHALPLAGLATMYTR"
     gene            complement(598..1314)
                     /locus_tag="VM638_03025"
     CDS             complement(598..1314)
                     /locus_tag="VM638_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVM11435.1"
                     /translation="MTGELDAVRQQLVVFAEEINQLYRQERQRTRELEQALGELNEAY
                     LSTMKTLAFLVEAKDVGTRMHLDRTQQYGLALAKTIDPELAARPELGYGFLLHDIGKI
                     GISERILNKQGPLTPSEWSVMKTHPLIGVQIVSPIRFLGEAVDVIRSHHERFDGTGYP
                     RGLRGEEIPLSARIFAVVDAFDAMTSDRPYREALPLDRAVGEITRGSGSHFDPEVVEA
                     FLLMMEDAAPVDPAPAASAG"
     gene            complement(1311..1679)
                     /locus_tag="VM638_03030"
     CDS             complement(1311..1679)
                     /locus_tag="VM638_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM11436.1"
                     /translation="MSRRKVLVVDDDPTIRDVLRAMLGFEGCEVEVASDGGSALEAVS
                     TMRPDIVLLDVMMPGTNGVEICRRLKADPSAPKVVMVTAKSTLEDEAAGLGAGADAYL
                     TKPFSPVQLLDIVGVEAAGA"
     gene            complement(1811..2350)
                     /locus_tag="VM638_03035"
     CDS             complement(1811..2350)
                     /locus_tag="VM638_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HVM11437.1"
                     /translation="MAEATEVPIAGGKRRIDRILEDSFVEGLGDLEMDELRRRRDLSR
                     AEREYLSLVRRLLHGRRDILQAELERRRGNGGSVLDRLPEILADDPGPSRGEAPTLAI
                     PDEELTLARRRVERLLADVALSDLENISDADLETGLARLDEEERSVSEARSRVLAVHD
                     ALQDELKERYRVKVRDLED"
BASE COUNT          357 a          900 c          858 g          349 t
ORIGIN      
        1 cgcgtgtaca tcgtcgcgag gccggccagg gggagcgcat ggaccggcac gccacagccg
       61 tcggtcccga tcgccgacgg ctcggcgccg gccgcgtcgg ggatcgcccg catgaccgcc
      121 cggtgcagcg gatgatcccg atcgaggtag ccggcagggt ccatctcggc ccgggagcag
      181 gcgagcagca tcccggcgtg cttcccggag cagtcgtggt accgggactc cggagcttcg
      241 acctcgcgag cccgcgccgg gtccatcggc cacgccggcg gacaccggag atctcccgcg
      301 cggcgtccgc cccgcgccag cagcgcctcg accgcgtcga ggtggatcgt ctcggcgttg
      361 tgggaggcgc acatcaccgc gacctcctcc tccgagagcg cctcctccac gaaggacagc
      421 gagacggccg cctgcagcgg cttcatgcac gagcgggcga acacgtcgcc ggacgggtca
      481 cccgaccagg cgagcagccc cccggcggca tccgcgaccg ccacggagcc gaggtgcacc
      541 gactcgacca cgccggagcg gatgacgcgg gcgagggggg cggacaacga gccggggtca
      601 gccggcggac gccgccggag ccggatcgac gggggccgca tcctccatca tcaggaggaa
      661 cgcctcgacg acctcagggt cgaagtggga gccggagccg cgggtgatct ccccgacggc
      721 ccggtccagc ggcagcgcct cgcggtaggg ccggtcgctc gtcatcgcgt cgaaggcatc
      781 cacgaccgcg aagatccgcg cggagagcgg gatctcctcg ccgcgcagac cgcggggata
      841 gccggtgccg tcgaagcgct cgtggtgcga gcggatcacg tcgaccgcct cgccgaggaa
      901 gcggatcggc gacacgatct ggacgccgat cagcgggtgg gtcttcatca ccgaccactc
      961 ggaaggggtc agggggccct gcttgttgag gatgcgctcg gagatcccga tcttcccgat
     1021 gtcgtgcagg aggaacccgt aaccgagctc ggggcgagcg gccagctccg ggtcgatcgt
     1081 cttcgcgagg gcgagcccgt actgctgggt ccggtcgagg tgcatgcgcg tcccgacgtc
     1141 cttcgcctcg acgaggaacg cgagcgtctt catcgtcgag aggtaagcct cgttcagctc
     1201 accgagcgcc tgctcaagct cgcgcgtgcg ctggcgctcc tgacggtaga gctggttgat
     1261 ctcttcggcg aagacgacga gctgctggcg gaccgcgtcg agctcgcccg tcatgcgccg
     1321 gcggcctcga cccccacgat gtccagcagc tgtacggggg agaacggctt cgtcaggtac
     1381 gcgtcggctc cggccccaag gccggcggcc tcatcctcga gcgtcgactt cgcggtcacc
     1441 atgacgacct tcggcgcgct cggatcggcc ttcaggcggc ggcagatctc gacgccgttc
     1501 gttccgggca tcatcacgtc gaggaggacg atgtcgggcc gcatcgtgct gaccgcctcg
     1561 agcgcggatc cgccgtcgga cgcgacttcg acctcgcacc cctcgaaccc cagcatcgcc
     1621 cgcaacacgt cacggatcgt cgggtcgtcg tccaccacga gcaccttgcg acgcgacatc
     1681 gtctcggttc gctccgatcc tccgaagggc ccgaccattg tgccagacca agagcgctcc
     1741 agcgtcaggt cgagatcatc caccgctccc cgttcttcgg ccgtcgcagg cccggccttg
     1801 agcggcgggg tcaatcctcc aggtcgcgga ccttcacccg gtaccgctcc ttcagctcgt
     1861 cctggagcgc gtcgtggacg gcgaggacgc gggagcgcgc ctccgagacc gagcgctcct
     1921 cctcgtccag ccgggcgagt ccggtctcga ggtcggcgtc gctgatgttc tcgaggtcgc
     1981 tcagcgcgac gtcggcgagg agccgctcca cccggcgtcg cgccaaggtc agctcctcgt
     2041 cggggatcgc gagcgtcggg gcctcgcccc gcgagggtcc ggggtcgtcg gcgaggatct
     2101 cggggagccg atccaggacg ctcccgccgt tgccgcgacg ccgctcgagc tcggcctgca
     2161 ggatgtcgcg gcgcccgtgg agcagccgcc ggacgagcga gaggtactcc cgttccgcgc
     2221 gcgacagatc ccggcggcga cggagctcgt ccatctccag gtcgcccaac ccctcgacga
     2281 aggaatcctc gaggatgcgg tcgatccgcc gcttcccccc ggcgatcgga acctcggttg
     2341 cctcggccac ggcgcacctc ccgtcgcggc gttcgccgct ccccggatta tcctcgaatc
     2401 cgccgccgct cgcgcggcta gcagacggcg cgcacgtagg ggtaggggtt gaccgctcct
     2461 cccc
//
LOCUS       DATLGG010000107         6647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_23940, whole genome shotgun sequence.
ACCESSION   DATLGG010000107 DATLGG010000000
VERSION     DATLGG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23940"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VM638_03040"
     CDS             complement(<1..400)
                     /locus_tag="VM638_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVM11438.1"
                     /translation="MELGKDLAGRRLGGRYLLDREIEAGGMASVWKARDDVLGRDVAV
                     KILHPDLGSDEEMYARFTREAVAAARLSHPAIVRVFDTGTDDGVCYIVMELVRGRTLA
                     DEIAEGPFEPARAAAVARGVLDALAHAHAAG"
     gene            complement(436..720)
                     /locus_tag="VM638_03045"
     CDS             complement(436..720)
                     /locus_tag="VM638_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11439.1"
                     /translation="MFARLQAQDVAETAAAEAVRANRTGGIDSARAAAEIAIGDKDPK
                     AELVEIEATAEGSVRVVVEKDASTLLIHRIDFLSGFARARGRATADPPPV"
     gene            complement(822..2567)
                     /gene="murJ"
                     /locus_tag="VM638_03050"
     CDS             complement(822..2567)
                     /gene="murJ"
                     /locus_tag="VM638_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HVM11440.1"
                     /translation="MADHRALDRVQHARPRRHPRRRPLPARPLGKEVAVGETLTPDPR
                     RGQEGGLVRSTALMSIGTALSRLTGFLRVAAMAFALGVAETRLADAYNVPNNVPNIIY
                     ELVLGGILSSVFVPVFVERLTAKDEKDPWHVARAVMTVTLLVLGVVMVIGILAAPAII
                     RLYTFRVEGPEREAAQAVATLFLRYFMPQIVLYGIGAVATGILNAHRRFAAPMFAPIL
                     NNLVVIAAFLIFAAMPAGPGTPAPGTITLAQKLVLALGTTGGVAAMTFALWPTLRRLG
                     FRFRWTLDLRHPSVRKLLRLSGWVVGYVVVNQIGLLIVIVLAGGVQGGYTAYQAAFIF
                     FQLPHAIFAVSIMTALLPSLSELWVREDRAGFRRILARGIRASAFVVVPAAAGYLVLA
                     RPIVRLLLEHGVAGAASTDLVSGVLVFFAIGLFPFSAFQLFLRAFYAMQDTRTPLLVN
                     VGAVTLNTAVNFALFPVLGVRGLALGHAVAYAFAALVAGIILRGRLGGLEGKSVAAGL
                     LKVAVAAAMTAAAAWSVAMVVAQGLGTEGFARQALQVGGGIVAGLVVFVVAALVLRME
                     EFDLVRRSILRRGRR"
     gene            complement(2450..4474)
                     /locus_tag="VM638_03055"
     CDS             complement(2450..4474)
                     /locus_tag="VM638_03055"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HVM11441.1"
                     /translation="MTRAGASLAIVLVLGPAIPTAAQPSPQARIALVNQDPLTGPRSP
                     LRISVEVTNTGTRAIERFGLTLHVYQPARSRSQYREAIAHDPTLSPILVTPIRTQETL
                     RPGETTTIAIRRRLPEIAQRDESALHPLKLQVESDGVPVTALRSVLVFIGERQEVPLD
                     LSLTVVLDERVLFDASGTFRGDRLERSLAAGGRLETTVAALEQAPMPVTVAIGPALLL
                     QLQRMSTGYRVRAANGSVREVGEEEPGAVRAAQMLERLEALVARGTVETIALPLASPS
                     IPALVDGQLRDDLELQMEGGTSVLRHFLPDATVAGTLVRPPRSLLTPRAIRVLERVGS
                     EGLVLDAEALAPAAEPRFSAVAVSAVEGGRGRTLPAIAPDAATMKHVLAPAPDPRLQA
                     QLALGEMAAVYFEQPGTPRGFALVIGDDDAPAEAVLSRLLGAIRRTSVSVSPRAGWLR
                     PRRATRLIDLARARGDLPRRRLEPRDLPRFSPAFLVEIRRARAAIGQLSSIADETAPL
                     VRRLQTQRLLAESAEFAEREAEGLAFLRSVRRRVALEFRKVAPPDDLSVTLTSHRGVI
                     PVTLSSRAEYPVRLRVTLVSPRLRFVEGATREVLLDRPRRAVTFPVLARTTGRFPVRV
                     LLQTPRGARIAESRITVRSTAYNTRALVVTLAAALFLLVLWGRRSLSARR"
     gene            complement(4471..4893)
                     /locus_tag="VM638_03060"
     CDS             complement(4471..4893)
                     /locus_tag="VM638_03060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVM11442.1"
                     /translation="MKTRREISAGGVVVRAGGSGAEALLAARRTRRGQLVWGLPKGNI
                     EPGESAEEAAVREVREETGYDGAIRAPLDEVTYWYVWAGVRVHKTVHFFLMDLLGGDP
                     SRRDHEMEDVAWFPLDEAAAVAAYDSEQRVLRLAAAAP"
     gene            complement(4890..5354)
                     /locus_tag="VM638_03065"
     CDS             complement(4890..5354)
                     /locus_tag="VM638_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HVM11443.1"
                     /translation="MKTSVDVHNYLVERDIPHELVPLQGRVRAPEHVAAVLGLPPQQV
                     GRVVLFEGGDAPVAAVVPADREPDPSRVAGTLGSGDLRRMDASSATEWSGFLEEALPP
                     AGLPEGTHIVVDRSLSDQEILYFPGGEVTSVLKIRPGDLLRAVDARVAQIAA"
     gene            complement(5351..>6647)
                     /locus_tag="VM638_03070"
     CDS             complement(5351..>6647)
                     /locus_tag="VM638_03070"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HVM11444.1"
                     /translation="LYLVGGSVRDAVRGVPHEDLDFATDARPEQVVRLLRGWAQHRYL
                     KGIRFGTVGAIRDEVAVEITTFREEVYARDSRHPDVTFARDIETDLSRRDFTINAMAV
                     RVPGGELIDPFRGLYDLGHRQLRTPLTPEVSFSDDPLRMLRACRFVATLGVVPAPEVV
                     EAIRSMKDRLAIVSVERIFDELMKLLPGEHVEAGLELMVESGLADVFLPELPALKLEQ
                     DPVHRHKDVFKHTLAVVAKCPPDPVLRLAALLHDVGKPKTRAITPEGVQFHHHEVVGA
                     RMAEERLRALRAPERVVQDVRQLVEMHLRFHTYRLGWTDGALRRYVRDAGPLIDRLNQ
                     LTRADCTTQNPRKARELAGLQDELEERIARLAEEENLASIRPPLDGNEIMEHLGIGPG
                     PIVGEAREHLLEARMDRGPMDKEEAYALLDAWAAERGMR"
BASE COUNT          997 a         2359 c         2448 g          843 t
ORIGIN      
        1 cgcccgcggc gtgcgcgtgg gcgagggcat ccaggacccc gcgggcgacg gcagcagcgc
       61 gggcgggctc gaagggaccc tccgcgatct cgtccgccag cgtccgtccg cggacgagct
      121 ccatcacgat gtagcacacg ccgtcgtccg tgccggtgtc gaagacgcgg acgatcgcgg
      181 ggtgcgagag gcgtgctgcg gcgaccgcct cgcgcgtgaa ccgggcgtac atctcctcgt
      241 ccgatccgag gtcggggtgg aggatcttca cggcgacgtc ccgcccgagg acgtcgtccc
      301 gcgccttcca gacggaggcc atgccgcccg cctcgatctc gcggtcgagg aggtagcgcc
      361 cgccgagccg gcgcccggcg agatccttcc ccagctccac gggggcagtc tacgggagca
      421 cgctgggcgc ccggctcaga cgggtggcgg atccgcggtc gcgcgcccgc gggcgcgggc
      481 gaacccgctg aggaagtcga tccggtggat cagcagggtg ctcgcgtcct tctcgacgac
      541 gacgcgcacg cttccctcgg cggtcgcttc gatctcgacg agctcggctt tcgggtcctt
      601 gtcgccgatc gcgatctcgg ctgccgcgcg tgcggagtcg atccccccgg tccggttcgc
      661 gcggaccgcc tcggcggcgg cggtttcggc cacgtcctgc gcctgaagcc tggcgaacat
      721 gaccgatgcg gtttcgacca cggtgagccc gacgagcgcg acgacgagga tggtcctgac
      781 cgcggagccg ctgacgaggc cgcgctcgtc acggaggggg ttcagcgacg cccccgtctg
      841 aggatgctcc gcctgaccag gtcgaactcc tccatccgaa gaaccagggc cgccacgacg
      901 aacactacca gccccgcgac gatcccgccc ccaacctgca gcgcctgacg cgcgaacccc
      961 tcggttccga gaccctgggc gacgaccatc gcgacgctcc aggcggccgc cgccgtcatc
     1021 gccgcggcga ccgcgacctt cagcaggccg gcggcgacgc ttttcccctc cagaccgccg
     1081 agccgcccgc ggaggatgat cccggccacg agcgcggcga aggcgtacgc cacggcgtgc
     1141 ccgagcgcca ggccccgcac gccgagcacc gggaagaggg cgaagttcac cgccgtgttg
     1201 agggtcaccg ctcccacgtt cacgagcagc ggggtccgcg tgtcctgcat cgcgtagaag
     1261 gcgcggagga agagctggaa cgccgagaac gggaacagcc cgatcgcgaa gaacacgagg
     1321 acccccgaca cgagatcggt ggacgcggcg ccggcgaccc cgtgctcgag caggaggcgc
     1381 acgatcggac gcgcgaggac gaggtacccg gcggctgcgg gaacgacgac gaaggcggat
     1441 gcgcggatgc cgcgcgcgag gatgcgccgg aacccggccc ggtcctcccg gacccagagc
     1501 tccgagagcg acgggaggag ggcggtcatg atcgacacgg cgaagatcgc gtgcgggagc
     1561 tggaagaaga tgaacgcggc ctggtaggcg gtgtacccgc cctggacgcc gccggcgagc
     1621 acgatgacga tcagcaggcc gatctggttg acgacgacgt acccgacgac ccagcccgag
     1681 aggcgcagga gcttgcggac cgacgggtga cgcaggtcga gcgtccagcg gaaccggaac
     1741 ccgagccggc gcaacgtggg ccagagcgcg aaggtcatcg cggccacacc gcccgtcgtg
     1801 ccgagcgcga ggacgagctt ctgggcgagg gtgatcgtcc ccggcgcggg ggtcccgggt
     1861 ccggcgggca tcgccgcgaa gatcaggaac gccgcgatga ccacgaggtt gttgaggatc
     1921 ggcgcgaaca tgggggccgc gaaccggcgg tgcgcgttca ggatcccggt ggcgacggcg
     1981 ccgatgccgt acagcacgat ctgcggcatg aagtaccgca ggaacagggt cgcgacggcc
     2041 tgcgccgcct cacgttcggg accctccacc cggaaggtgt agaggcggat gatggcgggc
     2101 gcggcgagga tgccgatgac catcacgacg ccgaggacca gaagggtcac cgtcatcacg
     2161 gcgcgcgcga cgtgccaggg gtccttctcg tccttcgccg tgagtcgctc gacgaacacc
     2221 gggacgaaga cggacgacag gatcccgccg agcacgagct cgtagatgat gttcgggacg
     2281 ttgttcggga cgttgtacgc gtcggcgagt cgggtctcgg cgacgccgag ggcgaacgcc
     2341 atcgcggcga cgcgcaggaa gccggtgagc cgcgagagcg cggtgccgat ggacatcagc
     2401 gccgtgctcc ggaccagccc gccctcttgc ccacgccgtg ggtcgggcgt cagcgtctcg
     2461 ccgacagcga cctccttccc cagaggacga gcaggaagag ggcggcggcg agggtgacga
     2521 cgagggcgcg cgtgttgtac gcggtcgagc gcacggtgat ccgcgattcg gcgatgcgcg
     2581 cgccccgggg cgtctgaagg agaacacgga ccgggaagcg cccggtcgtg cgtgccagga
     2641 ccgggaacgt gacggcccgc cgcggccgat cgagcagcac ctcccgcgtc gcgccctcga
     2701 cgaaccggag tcggggcgag acgagggtca cccgcagacg aacggggtac tcggcccggc
     2761 tgctgagggt caccgggatc acgccgcggt gcgacgtcag tgtcacggac agatcgtcgg
     2821 gcggtgcgac cttgcggaac tcgagcgcga cccgacgacg caccgaccgg aggaacgcga
     2881 gcccctccgc ctcccgctcc gcgaactccg ccgactcggc gaggaggcgt tgggtctgca
     2941 gccgccggac gagcggcgcc gtctcgtccg cgatcgagga gagctgtccg atcgccgcgc
     3001 gagcgcgccg gatctccacg aggaacgccg gagagaagcg cgggaggtcg cgcggctcga
     3061 gccggcgccg cggaaggtcg ccgcgggccc gcgcgaggtc gatcagacgc gtcgcgcgtc
     3121 gggggcggag ccagccggcg cgggggctga ccgagacgga tgttcggcgg atcgctccga
     3181 ggagccgcga caggacagcc tccgcgggcg cgtcatcgtc gccgatcacg agggcgaagc
     3241 cgcgcggggt tccgggctgc tcgaagtaca ccgcggccat ctcaccgagg gcgagctgcg
     3301 cctggaggcg tggatccggc gccggggcga gcacgtgctt catcgtcgcg gcatcgggcg
     3361 cgatcgccgg cagcgttcgg ccccgacccc cttcgaccgc ggataccgcg accgcgctga
     3421 accgcggctc agcggccggc gccagtgcct ccgcgtcgag caccagcccc tcgctgccca
     3481 cccgctcgag gacgcggatc gcccgcggcg tcagcagcga acgcgggggg cgcacgagcg
     3541 tcccggcgac cgtcgcgtcc ggaaggaagt ggcggagcac gctcgttcca ccctccatct
     3601 gcagctcgag gtcgtcccgc agctgcccat caacgagcgc cgggatcgac ggcgacgcga
     3661 ggggcagggc gatcgtctcg acggtgccac gggccacgag cgcctcgagc cgctcgagca
     3721 tctgcgcggc acggacggcg cccggctcct cctcacccac ctcccggacg ctcccgttgg
     3781 cggcgcgcac gcggtagccg gtgctcatcc gctgcagctg gagcaggagc gccggtccga
     3841 tcgcgacggt caccggcatc ggcgcctgct cgagggcggc gaccgtcgtc tcgagccgtc
     3901 caccggcggc gaggctgcgc tcgagccggt cgcctcggaa ggtgcccgac gcgtcgaaga
     3961 gcacccgctc gtcgagcacg accgtgagcg agagatcgag cgggacctcc tggcgttcac
     4021 cgatgaacac gagcacgctc cgcagggccg tcaccgggac gccgtcggac tcgacctgga
     4081 gcttcagggg atgcagggcg ctctcgtccc gttgcgcgat ctccgggagt cggcgccgga
     4141 tcgcgatcgt ggtcgtctca ccagggcgga gcgtctcctg cgtgcggatc ggtgtcacga
     4201 ggatcggcga aagcgtcgga tcgtgcgcga tcgcctcccg gtactgcgag cgggaacgcg
     4261 cgggctggta cacgtggagg gtgagaccga agcgctcgat cgcgcgggtc cccgtgttgg
     4321 tgacctcgac cgagatgcgc aggggactgc gcggtcccgt cagcggatcc tggttcacga
     4381 gcgcgatccg ggcctgtggt gaaggctggg cggccgtcgg gatcgccggc ccgaggacga
     4441 ggacgatggc cagcgaggcg cccgcccgcg tcatggggcc gccgcggcca gtcgcaggac
     4501 ccgctgctcg gagtcgtagg cggcaaccgc cgccgcctcg tcgagcggga accaggcgac
     4561 gtcctccatc tcgtggtccc ggcgggacgg gtcgcctccg aggagatcca tgaggaagaa
     4621 gtggaccgtc ttgtgcacgc ggacccccgc ccagacgtac cagtaggtca cctcgtccag
     4681 cggcgcacgg atcgcgccgt cgtagccggt ctcctcccgg acctcacgga ccgccgcctc
     4741 ctccgcgctc tcgccgggct cgatgttccc cttcggcagg ccccacacga gctggccccg
     4801 gcgggtacgg cgggcggcga gcagcgcctc tgctcccgat ccgccggccc ggacgacgac
     4861 cccaccggcc gagatctcgc gcctcgtctt cacgccgcga tctgggcgac ccgggcgtcg
     4921 accgcgcgga gcaggtcgcc cggcctgatc ttgaggaccg aggtcacctc gccgccgggg
     4981 aagtagagga tctcctggtc gctgagcgaa cggtccacga cgatgtgggt cccctcgggc
     5041 aggcccgctg ggggcagcgc ctcttccagg aagccgctcc actcggtcgc ggatgacgcg
     5101 tccatccgcc ggaggtcgcc tgatccgagc gtgccggcga ccctcgacgg gtccggctcc
     5161 cggtccgccg gcacgaccgc ggccaccggc gcgtccccgc cctcgaagag cacgacccgc
     5221 cccacctgct gcggtggaag cccgaggacg gcggcgacgt gctccggcgc gcggacccgc
     5281 ccctggagcg ggacgagctc gtgcgggatg tcgcgttcga cgaggtagtt gtggacgtcg
     5341 acggaggtct tcaccgcatc ccccgctccg cggcccacgc gtcgaggagg gcgtaggcct
     5401 cctccttgtc catcggaccc cggtccattc gcgcttccag caggtgctcg cgcgcctcgc
     5461 cgacgatcgg cccgggaccg atcccgaggt gctccatgat ctcgttgccg tcgaggggcg
     5521 gccggatcga cgcgaggttc tcctcctcgg cgaggcgagc gatccgctct tcgagctcgt
     5581 cctgcaggcc cgccagctct cgcgccttcc gcgggttctg ggtcgtgcag tcggcacgcg
     5641 tcagctggtt gagccggtcg atgagcgggc ccgcgtcgcg gacgtagcgc cgcagggcgc
     5701 cgtcggtcca cccgagccgg taggtgtgga agcgcaggtg catctccacc agctggcgga
     5761 cgtcctgcac cacccgctcc ggggcgcgga gggcccgcag ccgctcctcg gccatccgcg
     5821 cgccgacgac ctcgtggtgg tggaactgca cgccctccgg cgtgatcgcc cgcgtcttcg
     5881 gcttcccgac gtcgtgcagg agcgcggcga gccgcaggac cggatcgggt gggcacttcg
     5941 ccacgacggc gagcgtgtgc ttgaacacgt ccttgtggcg gtggacgggg tcctgctcga
     6001 gcttcaaggc gggaagctcc ggcaggaaga cgtccgcgag cccggactcg accatcagct
     6061 cgagcccagc ctccacgtgc tcgcccggca gcagcttcat cagctcgtcg aagatgcgct
     6121 cgaccgagac gatggccagc cggtccttca tcgagcggat cgcctcgacg acctccgggg
     6181 cggggacgac gccgagggtc gcgacgaacc ggcacgcgcg cagcatccgg agcgggtcgt
     6241 cggagaagga cacctccggg gtgagcggcg tgcggagctg gcgatggccg aggtcgtaca
     6301 agccgcggaa cggatcgatc agctcgcccc cggggacccg gaccgccatc gcgttgatcg
     6361 tgaagtcccg gcgggagaga tccgtttcga tgtcacgcgc gaaggtcacg tcgggatggc
     6421 gcgagtctcg cgcgtacacc tcttcacgga acgtcgtgat ctcgacggcg acctcgtccc
     6481 ggatcgcgcc cacggtgccg aagcggatcc ctttcaggta ccggtgctgc gcccaccccc
     6541 gcaggaggcg gacgacctgc tcgggccggg cgtcggtcgc gaagtcgagg tcctcgtgcg
     6601 ggaccccgcg aacggcatcg cggacggacc caccgacgag gtagagc
//
LOCUS       DATLGG010000108         3624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_23944, whole genome shotgun sequence.
ACCESSION   DATLGG010000108 DATLGG010000000
VERSION     DATLGG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23944"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VM638_03075"
     CDS             complement(<1..919)
                     /locus_tag="VM638_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVM11445.1"
                     /translation="MAGATVFGAGAAGTAIAIHLARTGSQVTLWGSRFDTRVLEAIEA
                     ERRHPALSEPIPETVRVLGPDDLKQAAADVDVAVMGANSAGARSLATMVVPALDDVPI
                     VVSLAKGLEPGTGLRTSEVYTETFPDSPVVALSGPSLAPEVAEGLLTAVAMASDDADA
                     LATVAERFRAPSFLVDTTDDVVGVEVCGVAKNVAAIAAGVLEGSGQQREQTMKNALSA
                     LFTRAAHEIAALAEALGGRRETAYGLAGIGDLLVTSLGGRNRLYGEAIGLGADPASTL
                     ADMTERGLTVEGADSARDVYELTRRTGVTM"
     gene            976..3105
                     /locus_tag="VM638_03080"
     CDS             976..3105
                     /locus_tag="VM638_03080"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HVM11446.1"
                     /translation="MTKPLPAVDIRDVQVVKNGATFLLTDRYGDVTEGNLAALGLYHR
                     DTRFLSRLDLRLNQQQPVLLHSSTEENHSQIIELAFPVPVGKSGGLEQRENMSLHRER
                     ILAGSLFERVRVRNFGREARHLVLDVGFAADFLDLFEVRGFTRERRGQLQAPAVERSQ
                     VVLRYVGLDGVTRTTTLRFSPMPTELRPGLASFELEVAAGAELEVAIEVIPQVGDETP
                     PRRSLREVEARLAREDMAWRKRGTRFLSSDPTLSRFLDRSVRDLRMLQSETDDGAFVL
                     DAGVPWYSTLFGRDAIITAYQMLTLDPSVAWAVLRTLAERQGTRDDPWREEEPGKILH
                     EVRSGELAASGEIPHGRYYGTIDATPLWLVLLAAAYRWTADLEAARALWPHALAALRW
                     IDEYGDRDGDGYLEYERRSTGGLENQGWKDSIDAICFPDGEPAEGPIALVEVQGYVYQ
                     AKRRLARVARDLGEDDVAERLGKEAEALRERFNRDFWVEREGYFALALDGEKRQVPTV
                     TSNPGHCLWSQIVDPALAPRVARRLTSPALLSGWGLRTLSRKHTAYDPLGYHRGSIWP
                     HDNAIVAHGLKRYGFDREAGMVLDQLSAAGRYFPYARFPELFCGFSSEEVPVPVQYPV
                     ACRPQAWSSGAALLMIRSYAGLTADAPRRRLFIVRPHLPRWVETVELRGLRVGDARLD
                     LVFSSHRGVTAVQVPRKQGDVEVLIQQ"
     gene            complement(3109..>3624)
                     /locus_tag="VM638_03085"
     CDS             complement(3109..>3624)
                     /locus_tag="VM638_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVM11447.1"
                     /translation="VRPECAPDPLCLIGTTGRGIGLFLGLGNGIFSPVPGGHSVGKVF
                     VDFQLADVNGDDRDDLVVPLPFEDQVRIVPGTASGFGDFYQVPAYEYPLGAGVADVTG
                     DGTADLVMDHGPAMISVAPGDGKGGFGEAHGYVARGATYTPKLADFTGDGKPDVLVPE
                     GDFAELFVQGR"
BASE COUNT          504 a         1320 c         1262 g          538 t
ORIGIN      
        1 gcatcgtgac gccggtccgt cgggtgagct cgtacacgtc gcgcgcggaa tcggcgccct
       61 cgacggtcag gccgcgctcg gtcatgtcgg cgagggtgct ggcggggtcg gccccgaggc
      121 cgatcgcttc gccgtacagc cggttgcgcc cgccgaggct cgtgacgagg aggtccccga
      181 tccccgcgag cccgtacgcg gtctcgcgcc ttccaccgag agcctccgcg agcgcggcga
      241 tctcgtgcgc ggcccgggtg aacaacgccg agagggcgtt cttcatcgtc tgctcgcgct
      301 gctgtccgct gccttcgagc accccggcgg cgatcgccgc gacgttcttc gcgacgccgc
      361 agacctcgac gccgaccaca tcgtcggtcg tgtccacgag gaagctcggc gcgcggaagc
      421 gctccgcgac ggtcgcgagc gcatcggcgt catcggaggc catcgcgacg gccgtcagca
      481 acccctccgc gacctcgggc gcgagcgacg gcccgctgag cgcgacgacg ggggagtcgg
      541 ggaaggtctc ggtatagacc tccgacgtcc gcaggcccgt ccccggctcc agtcccttcg
      601 cgaggctgac gacgatcggc acgtcgtcga gcgcgggcac caccatggtc gcgagtgacc
      661 gggcgccggc ggagttcgcc cccatgacgg cgacgtccac atcggctgcg gcctgcttca
      721 gatcgtcggg gccgaggacg cgaaccgtct ctgggatggg ctcgctcagc gcggggtgac
      781 gacgctccgc ctcgatcgcc tcgagcacgc gggtgtcgaa cctgctgccc cagagcgtca
      841 cctggctacc ggtgcgcgcc aggtggatcg cgatcgccgt tcccgccgct cccgctccga
      901 acacggtcgc cccggccacc cgcggagcat agcagggggt cggcggccgc tcgcggccgg
      961 gcgtacgatg ctgcggtgac gaagccgctg cctgcggtcg acatccgcga cgttcaggtc
     1021 gtgaagaacg gcgcgacctt cctgctgacc gatcgctacg gcgacgtgac cgagggcaac
     1081 ctcgcggccc tcggcctcta ccatcgcgac acgcggttcc tctcccggct cgatctccgt
     1141 ctgaaccagc agcaaccggt cctgctgcac tcctcgaccg aggagaacca ctcgcagatc
     1201 atcgagctgg cgttccccgt gccggtcggc aagtcggggg ggctcgagca acgcgagaac
     1261 atgtcgctgc accgcgagcg gatcctcgcc gggtcgctgt tcgagcgggt ccgcgtgcgc
     1321 aacttcggcc gtgaggcgcg ccacctcgtc ctcgacgtcg gtttcgcggc ggatttcctc
     1381 gacctgttcg aggtccgggg cttcacgcgg gagcgacggg ggcagctcca ggcgcccgcg
     1441 gtcgagcgga gccaggtcgt cctgcgctac gtcggtctcg acggcgtcac ccgcacgacg
     1501 accctgcggt tctccccgat gccgaccgag ctccgccccg gcctcgcgtc cttcgagctc
     1561 gaggtcgccg ccggcgccga gctggaggtc gcgatcgagg tcatcccgca ggtgggcgac
     1621 gagacccctc cgcggcgctc gctgcgcgag gtggaggcac ggctcgcgcg cgaggacatg
     1681 gcatggcgta agcgtgggac acggttcctc tcgagcgacc cgacgctctc gcggttcctc
     1741 gaccgctccg tccgtgacct ccggatgctg cagagcgaga ccgacgacgg ggctttcgtc
     1801 ctcgatgccg gcgtcccctg gtactccacg ctgttcggcc gcgacgcgat catcaccgcc
     1861 taccagatgc tcacgctcga cccctccgtc gcctgggcgg tcctgcggac gctcgccgag
     1921 cggcagggga cccgtgacga tccgtggcgg gaggaagagc ccgggaagat cctgcacgag
     1981 gttcgcagcg gagagctcgc cgcgtccggc gagatccccc acggccggta ctacgggacg
     2041 atcgacgcga cccccctatg gctggtcctg ctcgcggccg cgtaccggtg gacggcggac
     2101 ctcgaggcgg cacgcgcgct ctggccgcac gcgctcgccg ccctgcgctg gatcgacgag
     2161 tacggcgatc gcgacggcga cggctacctg gagtacgagc gtcgttcgac gggcggactc
     2221 gagaaccagg ggtggaagga ctcgatcgac gcgatctgct tccccgacgg ggagcccgcg
     2281 gagggaccga tcgctctcgt cgaggtgcag ggctacgttt accaggcgaa gcggcggctc
     2341 gcgcgggtcg ctcgcgacct cggtgaggac gacgtcgcgg agcgtctcgg aaaggaggcc
     2401 gaggcactgc gcgagcgctt caaccgcgac ttctgggtcg agcgggaggg atacttcgcg
     2461 ctcgccttgg acggcgagaa gcggcaggtt cccacggtga cgtcgaaccc cgggcactgc
     2521 ctgtggtcgc agatcgtcga tccggcgctg gccccacgcg tggcgcggag gcttacctca
     2581 ccggcgctgc tgagcggatg ggggctgcgc acgctcagcc gcaagcacac cgcctacgac
     2641 cccctcggct accatcgggg cagcatctgg ccgcacgaca acgcgatcgt ggcgcacggc
     2701 ctcaagcggt acgggttcga ccgtgaggcc ggcatggtcc tcgaccagct gagcgccgcc
     2761 ggccgttact ttccctacgc tcgcttcccc gagctcttct gcgggttctc ctccgaggag
     2821 gttccggtcc cggtgcagta cccggtggcc tgccggcccc aggcctggtc atccggggct
     2881 gccctgctga tgatccgctc ctacgccggt ctgaccgcgg acgcgccgag gcgccgcctc
     2941 ttcatcgtgc ggccgcacct cccgcggtgg gtcgagacgg tcgagctccg tgggctccgc
     3001 gtcggcgatg cccgcctcga cctcgtgttc tccagccacc gcggggtcac ggcggtccag
     3061 gtcccccgga aacaggggga cgtagaggtc ctgatccagc agtaacgtct agcggccctg
     3121 gacgaagagc tccgcgaagt ccccctccgg cacgaggaca tccggcttcc cgtcgccggt
     3181 gaagtctgcg agcttcggcg tgtaggttgc gccgcgagcc acgtagccgt gcgcttcccc
     3241 gaagccaccc ttcccgtcgc cgggggccac agagatcatg gccggcccgt ggtccatgac
     3301 gaggtccgcc gtcccgtccc ccgtgacgtc ggcgacgcca gcccccagcg ggtactcgta
     3361 cgccgggacc tgatagaagt ccccgaaccc cgaggctgtc ccgggcacga tccgcacctg
     3421 gtcctcgaac gggagcggca cgacgaggtc gtcccggtcg tcgccgttga cgtcggcgag
     3481 ttggaagtcg acgaagacct tcccgacgct gtgaccgccc ggcaccgggg agaagatgcc
     3541 gttcccgagc ccgaggaaga ggccgatccc ccgaccggtt gtcccgatca ggcagagcgg
     3601 gtccggcgcg cactccggcc gaac
//
LOCUS       DATLGG010000109         7090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_54514, whole genome shotgun sequence.
ACCESSION   DATLGG010000109 DATLGG010000000
VERSION     DATLGG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54514"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VM638_03090"
     CDS             complement(<1..1003)
                     /locus_tag="VM638_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVM11448.1"
                     /translation="MSIPLADGVPEPILRVFKGTPPTQQQWEAISHPLTPSAVVAGAG
                     SGKTAVMVARVVYLALVRAGVVEAGHRGAAPSEILCLTFTNKAAEELGRRVRHATEEL
                     RLPDGEEPTVHTYHAFAARMLDEYGLRMGLEPGGMLLTEAHKWQLAVSLLNDREFEHL
                     EVRTVRHVVENLLLLADECANHRVDPLEVAERSRAFAQELALERAGDRKIQLAALRRA
                     ELAEVAHAYGERKRALGVIDYADQIELACRLAEEHPEVGEAFRDRYKVVLLDEYQDTN
                     VAQAQLLSLLCGRGHPVMAVGDPDQNIYAWRGASLQNILRFHEDFSGGDPAERRPLYL
                     "
     gene            complement(1000..3909)
                     /locus_tag="VM638_03095"
     CDS             complement(1000..3909)
                     /locus_tag="VM638_03095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HVM11449.1"
                     /translation="MRFEPDDAQAPVLDLERGSLLVTGSPGCGKTTLLRERFARLVEA
                     GVDPERILLLVLNRRAARESRERLQGRLARSLPALPVLTAHGFAFSVVGRDFEALGYT
                     RPPEVLSASDQYAVVREMLRGERPADWPRFAPLLDVREFARQVADLCLRAQERLLGPD
                     ELEALAVDRSEYLEIAGFYRRYLDALHAANQTDFAGLLHGAVQLVRRGRVPEPGFEHV
                     LVDDYQDATHATEAIVAGLAPAARSVVVAADPEGHVFSYRGGSLEPLERIGDVLGGLR
                     RVELDGSHRLGPDVAALGRLPDVSEAERPPAARIRALEVPHPGEEAEVAARELLRRRV
                     EDDVAWERMAVIVRRYGAYLTALRHALRRHGVPFVVVAEESAVAAEPANRPVVDLFRY
                     VFQPEVRDELLEPLLGSRVGGLTPHQLRRLRREARVRRTKLVDLVEAPPQDLDRDLAE
                     PLLHFRRVIEELPRVAEGRGPDGAFFWLWTEHPVFGGMVADADARARDLDALSAFGDV
                     LGRFVERRPGAGVEDYLATLEAAEFGPDPWVLPEERHPHAVRVISAHRAHGIEVDVAV
                     VMGSIEGEFPSLSHRAPMVDLDRLVAPRTAVERIRDRLDEERALFRLAVSRARLSTLL
                     LASRSAGARNPRTPSRFAARLGLEWTRPERGAAPAASLREMEADLRRTLQDRTAPAPE
                     RLAALAALPETGADPRTWWWGRDWSDPGIPLHGEDEMYTSYSRLSSLENCALQYLYAS
                     EMGLDTDESFQMWLGSTVHEIIDDIQQGKVPRTEEAAHAELERRWDPDIFPNRAIEHR
                     RFLDGKEMLSLWLTHESPEIVASEKSFSFPIDGARIRGRIDAIFRMNNGKVRVVDYKT
                     SRWAPTKEDTRQSLQLAAYYLAMRRVEELAGYGEPGRLELHYLGKTHFREGYVRRSVI
                     PSQEDDYEAWAEGRLRELVEAVRAESFAPNPQADCQFCSFKPICPMWPEGQEVTP"
     gene            complement(3958..4314)
                     /locus_tag="VM638_03100"
     CDS             complement(3958..4314)
                     /locus_tag="VM638_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11450.1"
                     /translation="MRAEFFRPESPEETYGVASWDGVRAVVEASSPEAEQVLGRVFSP
                     APVAIDDASLRQPGAHGESVIEPGRLEWFRAAAMVRGAREGLGVRFASDAPGGWDPAG
                     TYRPMRQWVARKELPR"
     gene            complement(4364..5233)
                     /locus_tag="VM638_03105"
     CDS             complement(4364..5233)
                     /locus_tag="VM638_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVM11451.1"
                     /translation="MTRILRVLAPNPGPLTLDGTNTWVVGEQPSVVIDPGPDDPSHLL
                     GVLDEATPVGAVLLTHRHADHAEGARRFARAAGVEVYAFRPEGPERALADGDIVRAGA
                     VRVRVVHTPGHTQDHLAFFLDGPRALFTGDAVLGRGTSVVDPPEGDMAQYLESLGRMR
                     DLDPLTIYPGHGPVVFDAADRLEAYLAHRREREEQVWAALADGRKAPAQVVPGIYEGE
                     VDPSMFPLAERSVLAHLLKLEREGRVARSGRGQDVRFERIEPRACERCGRPAAKGSRY
                     CSRCAVSALQERP"
     gene            complement(5230..5895)
                     /locus_tag="VM638_03110"
     CDS             complement(5230..5895)
                     /locus_tag="VM638_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVM11452.1"
                     /translation="MDRPRPAATVVLVRPGDPGVEVLVERRSADSSFAPGFVVFPGGV
                     VEDEDRRLAEALFGDPSEDVRACAVRELYEETGILLEAGGARAVPERPPLLEALREAP
                     RQEDLAELARWVAPEFLAVRFDAWFYAVAAPPGVEPTPDGVEISDAWWTPPGAIVEAS
                     VRGDAPLMWPTLVTLEELEERRSVEEAVSTRIEQIPPPEPGVQPRRGVWLRPEYRADG
                     ASR"
     gene            5928..7022
                     /locus_tag="VM638_03115"
     CDS             5928..7022
                     /locus_tag="VM638_03115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVM11453.1"
                     /translation="MSERLRKLLIERIVTEGPITFADFMETALYDPEDGFYADSPIGA
                     DRHFVTSPHVSPAFGDLLARQLAQCWDALGRPERFDVVELGAGDGTLARQILRATAAS
                     PELTRAIRYVGVERSPGARAALTSAGLDAAPEAPDEIRGCVIANELLDNVPFHRLRER
                     DGRVLEIFVGADGGRLLEVEGEPTPEALAAAGAPFVPGEERPVSPAALKLVDRLTRTL
                     QRGYAFVFDYAFEPGRSTAPAHAYRDHRILADVLEEPGSQDVTAAVDLAAVTERASAA
                     GLQAWGPVPQEQALLALGFRLWAAGVRARQAEAEARGDWRSANRLFSERSRAGLLVDP
                     SNLGALQLVVFATRDLPTPAAILGDPDRGC"
BASE COUNT          970 a         2585 c         2612 g          923 t
ORIGIN      
        1 tcaggtagag gggccggcgc tccgcggggt ccccgcccga gaagtcctcg tggaagcgga
       61 ggatgttctg gaggctcgcg ccgcgccacg cgtagatgtt ctggtccggg tccccgaccg
      121 ccatgacggg gtggccgcgc ccgcacagca gggacagcag ctgggcctgc gcgacgttcg
      181 tgtcctggta ctcgtccagc aggaccacct tgtagcggtc ccggaaggcc tcgccgacct
      241 cggggtgctc ctcggcgagg cgacacgcga gctcgatctg gtccgcgtag tcgatgaccc
      301 ctagcgcgcg cttgcgctcc ccgtaggcgt gggcgacctc ggccagctcc gcgcgccgaa
      361 gggccgcgag ctggatcttc cggtcgccgg cccgctccag cgcgagctcc tgggcgaacg
      421 cgcggctgcg ctcggcgacc tcgagcgggt cgacgcggtg gttggcgcac tcgtcggcga
      481 gcaggaggag gttctccacc acgtgccgga ccgtgcggac ctcgagatgc tcgaactccc
      541 gatcgttcag gagggagacc gcgagctgcc acttgtgggc ctcggtgagg agcatccctc
      601 cgggctcgag gcccatccgc aggccgtact cgtccagcat ccgcgcggcg aaggcgtggt
      661 aggtgtggac ggtcggctcc tcgccgtcgg ggaggcgcag ctcctcggtg gcgtggcgca
      721 cccggcggcc gagctcctcc gctgccttgt tcgtgaaggt gaggcagagg atctccgagg
      781 gcgccgcgcc acggtggccc gcctcgacga caccggcacg cacgagcgcc aggtacacga
      841 cgcgcgcgac catgaccgcc gtcttcccgg accctgcgcc ggcgacgacc gccgacggcg
      901 tgagcgggtg gctgatcgcc tcccactgct gctgcgtcgg cggggtgccc ttgaagaccc
      961 gcaggatggg ctcggggacg ccgtcggcga gcgggatgct cacggcgtca cctcctggcc
     1021 ctcgggccac atggggcaga tgggcttgaa cgagcagaac tggcagtcgg cctgcgggtt
     1081 cggggcgaac gactccgcgc ggaccgcctc gacgagctcc cgcaggcggc cctcggccca
     1141 cgcctcgtag tcgtcctcct gcgaagggat gacgctgcgg cgaacgtagc cttcgcggaa
     1201 atgggtcttg ccgaggtagt ggagctcgag cctgcccggc tcgccgtacc ccgcgagctc
     1261 ctcgacgcga cgcatcgcga ggtagtaggc ggcgagctgg aggctctgtc gggtgtcctc
     1321 cttcgtcgga gcccagcggc tcgtcttgta gtcgaccacg cggaccttgc cgttgttcat
     1381 gcggaagatc gcgtcgatcc tcccgcggat ccttgcgccg tcgatcggga acgagaagct
     1441 cttctccgag gcgacgatct ccggcgactc gtgggtcagc cagagcgaga gcatctcctt
     1501 gccgtccagg aaccggcgat gctcgatcgc gcggttcggg aagatgtcgg ggtcccaacg
     1561 gcgctcgagc tccgcgtgcg cggcctcctc ggtgcgcggg accttcccct gctggatgtc
     1621 gtcgatgatc tcgtggacgg tcgatccgag ccacatctgg aagctctcgt cggtgtcgag
     1681 gcccatctcg gaggcgtaga ggtactgcag cgcgcagttc tcgagggagg aaaggcgaga
     1741 gtagctcgtg tacatctcgt cctcgccgtg gagggggatc cccgggtcgc tccagtcgcg
     1801 gccccaccac caggtgcgcg ggtcggcacc ggtctccggc aacgccgcca gcgcggcgag
     1861 ccgctcgggc gcgggagccg ttcggtcctg gagcgtcctg cggagatccg cctccatctc
     1921 gcggagggac gcggccgggg cggcgcctcg ctcggggcgc gtccactcga ggccgaggcg
     1981 ggcggcgaac cgcgatggcg tgcgcgggtt gcgggcgccg gcggagcggc tggcgagcag
     2041 gagcgtgctg aggcgcgcgc ggctgaccgc gaggcggaag agcgcccgct cctcgtcgag
     2101 gcgatcgcgg atccgctcca cggccgttcg gggcgcgacg aggcggtcga gatccaccat
     2161 cggcgcccga tgggacaggc tcgggaactc accctcgatc gagcccatga cgaccgcgac
     2221 gtccacctcg atgccgtgcg cgcggtgggc cgagatgacc cggacggcgt gtgggtgccg
     2281 ctcctctggg aggacccagg ggtcgggccc gaactcggcg gcctcgagcg tcgcgaggta
     2341 atcctcgaca cctgcgcccg gccgccgctc cacgaaccgg ccgagcacgt cgccgaacgc
     2401 tgagagcgcg tcgaggtccc gcgcgcgcgc gtccgcgtcg gcgaccatcc cgccgaagac
     2461 ggggtgctcc gtccagagcc agaagaaggc gccgtcggga ccgcggccct ccgcgacacg
     2521 cggcagctcc tcgatgaccc ggcggaagtg gaggagcggc tcggcgaggt cgcggtcgag
     2581 gtcctgcggg ggtgcctcca cgaggtcgac gagcttcgtg cggcggacgc gcgcctcgcg
     2641 acggaggcgg cggagctggt gcggcgtgag gcccccgacc cgcgagccga ggagcggctc
     2701 gaggagctcg tcgcggacct ccggctggaa gacgtagcgg aagaggtcga cgaccgggcg
     2761 gttcgccggc tcggccgcga ccgccgactc ctccgcgacg accacgaagg ggaccccgtg
     2821 gcgccggagg gcgtggcgga gcgcggtgag gtacgcgccg tagcggcgga cgatcacggc
     2881 catccgctcc cacgcaacgt cgtcctcgac gcggcggcgg aggagctccc gcgcggcgac
     2941 ctcggcctcc tcgcccgggt gtgggacctc gagggcgcgg atccgagccg cgggcggacg
     3001 ctccgcctcc gagacgtccg ggagacgtcc gagggcggcc acgtcggggc cgaggcggtg
     3061 actcccgtcg agctcgaccc ggcggaggcc accgagcacg tcaccgatcc gctccaaggg
     3121 ctcgaggctg cccccgcggt aggagaagac gtgcccctcc gggtcggcgg cgacgacgac
     3181 gctccgcgcc gcgggagcga gtcccgcgac gatcgcctcg gtcgcgtgcg tcgcgtcctg
     3241 gtagtcgtcg acgaggacgt gctcgaagcc gggctcggga acgcgtccgc ggcgaacgag
     3301 ctggacggcc ccgtggagga ggccggcgaa gtcggtctgg ttcgcggcat ggagcgcgtc
     3361 gaggtagcgg cggtagaacc ccgcgatctc gaggtactcc gaccggtcga cggcgagcgc
     3421 ctcgagctcg tcggggccga ggaggcgctc ctgggcgcgc agacagaggt ccgcgacctg
     3481 tcgcgcgaac tctcggacgt cgaggagcgg ggcgaagcgt ggccagtccg cgggccgctc
     3541 gccgcggagc atctcccgga cgacggcgta ctggtcggag gccgagagca cctccggcgg
     3601 acgggtgtac ccgagcgcct cgaagtcgcg gccgaccacc gagaacgcga acccgtgcgc
     3661 ggtgaggacg ggcagggccg gcagggagcg cgccaacctg ccctggagcc gctcgcggct
     3721 ctcccgcgct gcgcgccggt tcaggacgag gagcaggatg cgctcggggt cgacgcccgc
     3781 ctcgaccagc cgggcgaatc gctcccgcag gagcgtcgtc ttcccgcagc cgggcgagcc
     3841 ggtgaccagg aggctccccc gctcgaggtc gagcacgggg gcctgcgcgt cgtcgggctc
     3901 gaacctcacg actgcactgt accgggaggg tctgacacgc ggttcagcgg aggagcgcta
     3961 gcggggcagc tccttgcgcg cgacccattg gcgcatcggg cggtaggtgc cggcggggtc
     4021 ccaaccgccg ggggcgtcgg aggcgaaccg aaccccgaga ccttcacgcg cgccgcgaac
     4081 catggccgcc gcgcggaacc actcgagtcg tccgggctcg atcacgctct caccgtgcgc
     4141 accgggctgt cggagcgacg cgtcgtcgat cgccaccggc gccgggctga acacgcggcc
     4201 gaggacctgc tcggcctccg ggctcgatgc ctcgacgacc gcgcggacgc cgtcccacga
     4261 ggcgaccccg tacgtctcct cgggcgactc gggtcggaag aactcggctc gcattccgcg
     4321 gaggataccg cacggccggg acgggaccgc tcccgcgacg cgatcagggc cgctcttgga
     4381 gggccgacac ggcgcagcgg ctgcagtagc ggctgccctt cgccgccggt cgcccacagc
     4441 gctcgcaggc ccggggctcg atgcgctcga accgcacgtc ctggccgcga cccgagcgcg
     4501 ccacgcgacc ctcgcgctcg agcttcaaca ggtgggcgag gaccgagcgc tcggccagcg
     4561 ggaacatcga cgggtccacc tcgccctcgt agatcccggg aacgacctgg gccggtgcct
     4621 tccgtccgtc tgccagcgcc gcccacacct gctcctcccg ctcgcgtcgg tgcgcgaggt
     4681 acgcctcgag ccggtccgca gcgtcgaaga cgaccggccc gtgcccggga tagatcgtca
     4741 ggggatccag gtcccgcatc cgcccgaggg attcgaggta ctgcgccatg tccccttccg
     4801 gcggatcgac gacgctcgtg ccgcgcccga gcacggcatc ccccgtgaac agggcgcgcg
     4861 gcccgtcgag gaagaacgcg aggtggtcct gcgtgtgccc cggcgtgtgg acgacgcgga
     4921 cgcggaccgc tcccgcccgg acgatgtcgc catcggccag cgcccgctcg ggaccctccg
     4981 ggcggaacgc gtacacctcc acgccggcgg cacgggcgaa ccggcgtgcg ccttccgcgt
     5041 ggtcggcgtg acggtgggtc agcaggaccg cccccacggg agtcgcctcg tccaggaccc
     5101 cgaggaggtg gctcgggtcg tccggcccgg ggtcgatgac gaccgacggc tgctcgccca
     5161 ccacccacgt gttggtcccg tcgagggtga ggggacccgg attcggggcg aggacccgca
     5221 agatccgcgt catcggctcg cgccgtccgc gcggtactcg gggcgcagcc acaccccacg
     5281 ccgaggctgg acccccggct ccggtggagg gatctgctcg atccgcgtgg acaccgcctc
     5341 ctcgacggag cgccgttcct caagctcctc gagcgtgacg agcgtcggcc acatgagcgg
     5401 cgcgtcaccc cgcacgctcg cctccacgat cgctccgggc ggggtccacc aggcgtcgga
     5461 gatctcgacc ccgtccggcg tcggttccac acccggtggc gccgcgaccg cgtagaacca
     5521 cgcgtcgaag cgcaccgcga ggaactccgg cgcgacccat cgggccagct cggccaagtc
     5581 ctcctgccgg ggagcctcgc gcagcgcctc gagcagcggc ggtcgctcgg gcaccgcgcg
     5641 agcgccgccg gcctcgagca ggatcccggt ctcctcgtag agctcccgga ccgcgcaggc
     5701 gcggacgtcc tcggacggat cgccgaacag cgcctccgcc agccgacggt cctcgtcctc
     5761 gacgacccca ccggggaaga cgacgaaccc gggagcgaac gaggaatccg ccgagcgccg
     5821 ctcgaccagc acctcgacgc ccggatcccc cgggcggacg agcaccacgg tggcggcggg
     5881 gcgcggccgg tccatcgcgc gcattctgcc agggggagaa tggcggcgtg agcgaacggc
     5941 tgcgcaagct cctcatcgag cggatcgtca cggagggccc gatcaccttc gccgacttca
     6001 tggagaccgc gctctacgac cccgaggacg ggttctacgc cgactcgccg atcggggccg
     6061 accggcactt cgtgacgagc ccgcacgtct cgccggcctt cggcgacctc ctcgcacgcc
     6121 agctcgcgca gtgctgggac gccctcgggc ggcccgagcg gttcgacgtc gtcgagctcg
     6181 gcgcgggcga cgggaccctg gcgaggcaga tcctgcgtgc gaccgcggcc tcccccgagc
     6241 tgacgcgcgc gatccgttac gtcggtgtcg agcgctcgcc cggtgcgcgg gcggccctca
     6301 ccagcgccgg tctcgacgcg gccccggagg cgccggacga gatccgaggg tgcgtgatcg
     6361 ccaacgagct cctcgacaac gtccccttcc accgcctgcg ggagcgcgat ggccgggtcc
     6421 tcgagatctt cgtcggcgcc gacggcggac ggctcctgga ggtcgagggc gagcccaccc
     6481 ccgaggcgct cgcggccgcc ggagcgccct tcgtccctgg cgaggagcga ccggtctcac
     6541 ccgccgcgct caagctcgtc gaccggctca cccggacgct gcagcggggg tacgccttcg
     6601 tcttcgatta cgccttcgag ccgggtcgat cgacggcccc cgcccacgcc tatcgcgacc
     6661 accgcatcct cgccgacgtg ctcgaagagc ccggcagcca ggatgtgacc gcggccgtgg
     6721 atctggccgc ggtcaccgag cgtgcgagcg ccgccggcct ccaggcctgg ggcccggtgc
     6781 cgcaggagca ggcgctgctc gccctcgggt tccgcctgtg ggccgcgggc gttcgagcgc
     6841 gccaggcgga agcggaggct cgaggggact ggcgctcggc caaccgcctc ttctccgagc
     6901 gcagccgcgc gggcctgctc gtcgacccgt cgaacctcgg cgcgcttcag ctcgtcgtct
     6961 tcgcaacccg cgatctgccg accccggcgg cgatccttgg agatcccgac cgcggctgct
     7021 gaagctccga tctcgagcct taccaagcta agtgcggtac gatgactgtg gtcaggaggc
     7081 gaccatgccc
//
LOCUS       DATLGG010000110         6408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_24012, whole genome shotgun sequence.
ACCESSION   DATLGG010000110 DATLGG010000000
VERSION     DATLGG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24012"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1428)
                     /gene="ffh"
                     /locus_tag="VM638_03120"
     CDS             complement(88..1428)
                     /gene="ffh"
                     /locus_tag="VM638_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HVM11454.1"
                     /translation="MFEQLTSRLTPVFKKLRSRGKLHPKQVDNALAEIRIALLEADAA
                     AEVADAMLGRVRERALSEEVMRSITPGQQVVKIVNDELAETLGGKHRPFELPNASPAV
                     VMMAGLQGSGKTTACAKLALHLREKGKRPLLAAADLLRPAAVEQLRTLGEEIGVPVVS
                     EGRDPVRVAKAALKEASRRGHDVLIVDTGGRLHIDEEMMREARRIRDVLRPHHVLMAA
                     DAMTGQDALRQARAFMREVDLTGFVLTKLDGDARGGAALSVTAATGRPIFFAGVGERA
                     KDLEVFHPDRMASRILGMGDVVSLVEKAQREMDEERAREQAERIMQARFTLEDFLEQL
                     RQVRKLGPLKDLMGMLPGAGELAAVDIDEREVARTEAIISSMTPEERQSPEIINGSRR
                     ARIAGGSGTSVAQVNALLKQFDQARTMMQRLTGGGLKGLRGLPGLPGLPGMPPR"
     gene            complement(1489..1977)
                     /locus_tag="VM638_03125"
     CDS             complement(1489..1977)
                     /locus_tag="VM638_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11455.1"
                     /translation="MTEPPAAPGTPPPQPAAGTSTGQPSGAVMTAGILQIVIGGLIAL
                     FTLYGMVAAGGAVFAVGAAAGMVIIILLYGLAQLLAGIFTVQRKNVGRILGMVLGVIG
                     ALLFLLGMISSFNQTTGFDFETGQVTSGMNVGGVIINLLLMAANVVIVILLARNGREF
                     TR"
     gene            complement(2045..2698)
                     /locus_tag="VM638_03130"
     CDS             complement(2045..2698)
                     /locus_tag="VM638_03130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM11456.1"
                     /translation="MPQPSADRFDEAYFRQLYGEIPAQTPFDRARDARIVHLVHRHAR
                     PGGALLDIGCGYGFLLGRFRGRHRLVGIELSHHAGSTAREGAAGAAGARLVRADVEGG
                     LPFRRTFEVVLAVNVIEHLREPASAVASIREILLPGGLCVVHLPTVSGPFSRAIYRLA
                     YAQDPTHIYRPSAREVDRLFASEGFELLESSHAPHAPKIVARRLTGSPAYLAAFRAP"
     gene            2790..4562
                     /locus_tag="VM638_03135"
     CDS             2790..4562
                     /locus_tag="VM638_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM11457.1"
                     /translation="MSTHSTPHREDLARLEATLVRIRWFATAFGLFQVWQGATVPPLP
                     PAGVISVAYALVALLGLGNVAIWLGLRREHSLDGLRRIGLAAFILDIVVVTGTCWAYS
                     YDRYGATWVLAYVLPLEGAMRYQLRGALAAVAVAFATELAREAYRTHLFPDLGFHLSS
                     VTFRVGVMALVALVAGMMARSLAEQARAAERRARAFEDLARRERAARRELSAFHDTVL
                     AGIGGQTPQEALQLMADSIGSNLGYESLCILAWDDDDLREEASFGDRHRHDDPVTQAT
                     LVAAARTQRPQLTDGGRQMAVAMAIGGKTIGVIHVQAAFEGGLGDGEVEALRRLADQV
                     ALVAEGARLHAERAQTVERLEELARLKSDFVAITSHELRTPLTGIRGFASILFKQHDT
                     LSPEERIEYLGIVDQQSARLSRLVEDLLVVTKLDDGRLKLSIREAEVEPFVHGVVASF
                     AGEAARIHVVSEEAPRWARFDPDRVEQVLRNLVHNALKFSAESDPVVVRVGLADEALL
                     FEVADRGVGMSTDQRERIFDRFHQAGNGHRREKEGVGLGLYIARRLVDEMGGAIGVES
                     TPGAGTTFRFTLPQAVAAKTAERT"
     gene            complement(4559..5542)
                     /gene="ftsY"
                     /locus_tag="VM638_03140"
     CDS             complement(4559..5542)
                     /gene="ftsY"
                     /locus_tag="VM638_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HVM11458.1"
                     /translation="MPSGVGLEIVVIAGLLVLLFVGLVVGRLRAHEPTPAPRADAASA
                     PQTDGLGSRVRRWLGSGKPTEDDWTALEEALIAADAGPRAAREIVTRVRERFEPGEDP
                     ERLVIEEIAGMFEGDPGLSLEGRPAVIVVVGVNGTGKTTTIGKLANRLVGQGRLVALA
                     ASDTYRAAAIPQLAEWAERAGADLVAQGRGADPAAVAFDAAEAARARGHDVLIVDTAG
                     RLHSEQPLMDELGKVKRALGKAAGDVDEVLLVLDATTGQNGIAQARAFVDAVTVTGLA
                     LTKLDGTAAGGVVLAVREELGIPVKLVGTGEGIDDLRAFDPASFAEALVRG"
     gene            complement(5550..>6408)
                     /locus_tag="VM638_03145"
     CDS             complement(5550..>6408)
                     /locus_tag="VM638_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM11459.1"
                     /translation="VGTNEEWREAASLLQRLRDEYEDEDQARRDLERRIGEAERLLRD
                     GHGKDPAEAVAALDEDDTVEALQKRADLVARRLTLLGRVNLVANEEYTALQERHDFLQ
                     REIDDVKAARRDLLEVVREVDRRIVEIFDAAFHDVATEFERMFQTVFPGGEGRIVLTE
                     GEDLLTTGIEIEARPGRKRVKRLSLLSGGERALTALAFLFAIFKARPSPFYLLDEVEA
                     ALDDVNLHRFIQLIRDFARDSQVLVVTHQKRTMEAADVLYGVSMGRSGATTVITQRVA
                     EAVPEPAAR"
BASE COUNT          904 a         2347 c         2239 g          918 t
ORIGIN      
        1 catccgcatc agcctgatct tcaccatctc gaaggatcct ctcctcgggt cggcagcgat
       61 gatacccggc ccgctcgggg ccggcggcta ccgcggcggc atgcccggca gccccgggag
      121 acccggcagc ccgcggagcc ccttcagccc gccgcccgtc aggcgctgca tcatcgtccg
      181 cgcctggtcg aactgcttca gcagcgcgtt gacctgcgcc accgacgtcc cgctcccgcc
      241 ggcgatccgc gcgcggcggc tcccgttgat gatctccggg ctctgccgct cctccggggt
      301 catggacgag atgatcgcct cggtccgcgc gacctcgcgc tcgtcgatgt cgacggcggc
      361 aagctcgccg gcgcccggca gcatccccat caggtccttg aggggcccca gcttgcgcac
      421 ctgccgcagc tgctcgagga agtcctccag cgtgaagcgc gcctgcatga tccgctcggc
      481 ctgctcgcgc gcgcgctcct cgtccatctc acgctgcgcc ttctccacga gcgagacgac
      541 gtcgcccatc ccgaggatcc gcgaggccat ccggtcggga tggaacacct cgaggtcctt
      601 tgcacgctcc ccgacgccgg cgaagaagat cggccgcccg gtcgccgccg tcaccgacag
      661 cgcggcgcca ccgcgcgcgt caccgtcgag cttcgtgagg acgaagccgg tgaggtcgac
      721 ctcccgcatg aaggcgcgcg cctgccggag ggcgtcctgt cccgtcatcg cgtcggccgc
      781 catgagcacg tggtgcggcc gcaggacgtc ccggatccgc cgagcctcgc gcatcatctc
      841 ctcgtcgatg tggagccggc ctcccgtgtc cacgatcagc acgtcgtggc cccggcggct
      901 cgcttccttc aacgccgcct tcgccaccct gaccgggtcc cggccctcgg atacgacggg
      961 gacgccgatc tcctccccga gcgtccgcag ctgctcgacc gcggccgggc ggagcaggtc
     1021 ggcggcggcg agcaggggcc gcttgccctt ctcgcggagg tggagggcga gcttcgcgca
     1081 cgccgtcgtc ttcccggagc cctggagccc ggccatcatc acgacggccg gagaggcgtt
     1141 cggaagttcg aaggggcggt gcttcccgcc cagcgtctcc gccagctcgt cgttcacgat
     1201 cttcacgacc tgctgacccg gggtgatgct ccgcatcacc tcctcggaca gcgcccgctc
     1261 ccggacgcgg cccagcatcg cgtcggcgac ctcggcggcg gcgtcggcct cgaggagcgc
     1321 gatccggatc tccgccagcg cgttgtcgac ctgcttcgga tggagcttcc cgcgggatcg
     1381 gagcttcttg aagaccggcg tgagccggct cgtgagctgc tcgaacatca tccggaacgg
     1441 tagcagcccc cggcgcatgc cgggggctgc cgagcctgat ggagcgggct accgggtgaa
     1501 ctcgcgcccg ttgcgcgcca acaggatgac gatcacgacg ttcgcggcca tcaggagcag
     1561 gttgatgatg acgccgccga cgttcatccc gctcgtgacc tgcccggtct cgaagtcgaa
     1621 accggtggtc tggttgaacg aggagatcat cccgaggagg aagagcaggg ctccgatgac
     1681 gccgagcacc atgccgagga tgcgtccgac gttcttccgc tgcaccgtga agatgccggc
     1741 gagcaactgc gcgaggccgt acaggaggat gatgatcacc atccccgcgg cggctccgac
     1801 ggcgaagacc gcccctcccg cggcaaccat cccgtacagc gtgaacagag cgatcaaacc
     1861 gccgatcacg atctggagga tccccgcggt catcacggcg cccgaaggct gtcccgtaga
     1921 cgtgcctgcg gcgggctgcg gcggcggggt tccgggtgcg gcgggtggtt cggtcatgct
     1981 gtaccccctt cctgggctgc ggtcggcctg actctaccag ggggttctcg acggtccaag
     2041 cacctcacgg cgcccggaaa gccgcgaggt aggccgggct cccggtgagc cgccgggcga
     2101 cgatcttcgg cgcgtggggg gcgtgggacg actccaggag ctcgaacccc tccgacgcga
     2161 acaggcgatc cacctccctg gccgacggcc ggtagatgtg cgtggggtcc tgcgcgtacg
     2221 ccaaccggta gatcgcccgg ctgaagggcc cgctgacggt ggggagatgg acgacgcaga
     2281 gcccgcccgg caggaggatc tctcggatcg acgcaacggc cgatgcgggc tctcgcaggt
     2341 gctcgatcac gttgaccgcg aggacgacct cgaaggtccg acggaacggc aggcccccct
     2401 cgacatcggc gcgcaccagc cgcgcccccg ctgcgccggc cgctccctct cgggcggtgg
     2461 accccgcgtg gtgcgacagc tcgatcccga cgaggcggtg ccgcccccgg aaccgcccga
     2521 gcaggaaccc gtagccgcag ccgatgtcga ggagagcgcc gcccggccgt gcgtggcggt
     2581 ggacgagatg cacgatccgg gcgtcccgcg cgcgatcgaa cggcgtctgc gccgggatct
     2641 cgccgtagag ctggcggaag tacgcctcgt cgaaacggtc ggcggagggc tgcggcacgg
     2701 ccgcaacgat ggggcgtggg gtgacggggg tcaagccggc ccccccaccg gccgaaaccc
     2761 cagagaggcc cgcggtcggg aggagccggg tgagcacgca cagcacgccg catcgcgagg
     2821 atctcgcgcg cctcgaggcg accctcgtgc ggatccgctg gttcgcgacg gccttcgggc
     2881 tcttccaggt ctggcagggg gcgaccgttc cgccgcttcc cccggccggg gtcatctcgg
     2941 tcgcgtacgc gctcgtcgcc ctgctcggcc tcggcaacgt cgcgatctgg ctggggctcc
     3001 ggcgcgaaca ctcgctcgac ggtctgcgaa ggatcgggct tgcggccttc atcctggaca
     3061 tcgtcgtcgt caccggcacc tgctgggcgt actcctatga ccgctacggc gcgacctggg
     3121 tgctcgcgta cgtcctgccc ctcgaaggag cgatgcgcta ccagctccgc ggcgccctcg
     3181 cggcggtcgc cgtcgccttc gccacagagc tggcgcgcga ggcgtaccgg acgcacctgt
     3241 tccccgatct cggcttccac ctctcgtcgg tgacgttccg cgtcggcgtg atggcgctcg
     3301 tcgcactggt cgccgggatg atggccaggt cactcgcgga gcaggcgcgc gcagccgagc
     3361 gacgcgcccg agccttcgag gaccttgcgc gacgggaaag agccgcgcgg cgcgagctgt
     3421 cggcgttcca cgacacggtg ctcgcaggga tcggcggcca gacgccacag gaggcgcttc
     3481 aattgatggc cgactcgatc ggctcgaacc tcggatacga gagcctctgc atcctcgcct
     3541 gggacgacga cgacctccgg gaggaagctt cgttcggcga ccgccaccgt cacgacgacc
     3601 cggtgacgca agcgacactc gtggccgccg ccaggacaca gcggccgcag ctgaccgacg
     3661 gcggccggca gatggccgtc gcgatggcga tcggcggcaa gacgatcggg gtgatccacg
     3721 tccaggcggc cttcgagggc gggctcggcg acggcgaggt cgaggccctc cgccggctcg
     3781 ccgaccaggt cgcgctcgtc gccgagggcg cccggctcca cgccgagcgc gcgcagaccg
     3841 tcgagcggct cgaggagctg gcccggctca agtccgactt cgtggcgatc acgagccacg
     3901 agctccgcac cccgctcacc gggatccgcg ggttcgcgag catcctgttc aagcagcacg
     3961 acacgctctc ccccgaggaa cggatcgagt acctcgggat cgtcgaccag cagagcgcgc
     4021 ggctctcccg cctcgtcgag gatctgctcg tcgtcacgaa gctcgacgac gggcggctga
     4081 agctctcgat ccgcgaagcc gaggtcgaac ccttcgtcca cggcgtggtc gcctcgttcg
     4141 ccggggaggc cgccaggatc cacgtcgtgt cggaggaggc gcctcggtgg gcccgcttcg
     4201 accccgatcg ggtcgagcag gtcctgcgga acctcgtgca caacgcgctc aagttctccg
     4261 ccgagagcga tcccgtggtc gtgcgggtcg gtctcgccga cgaggctctc ctgttcgagg
     4321 tggccgaccg gggcgtcggg atgagcaccg accagcggga aaggatcttc gaccgcttcc
     4381 accaggccgg gaacggccat cgccgcgaga aggaaggggt cgggctcggt ctctacatcg
     4441 cgcgccgcct cgtcgacgag atgggcgggg cgatcggcgt cgagtcgacg cccggagccg
     4501 gcacgacgtt ccgcttcacg ctcccgcagg cggtcgccgc gaagaccgcc gagcgcacct
     4561 agccgcggac gagcgcctcc gcgaaggagg ccgggtcgaa cgcgcggagg tcgtcgatcc
     4621 cctcaccggt cccgaccagc ttcacgggga tccccagctc ctctcgcacc gcgagcacga
     4681 cgccaccggc cgcggtgccg tcgagcttcg tgagggccag gccggtgacc gtcaccgcat
     4741 cgacgaaggc cctcgcctga gcgatcccgt tctgtcccgt cgtggcgtcc agcacgagca
     4801 ggacctcgtc gacgtcaccg gcggccttcc cgagcgcgcg cttcaccttc ccgagctcgt
     4861 ccatcagcgg ctgctcggag tggagacggc cggccgtatc gacgatcagg acgtcgtggc
     4921 cccgggcgcg ggcggcctcg gcggcgtcga acgccaccgc cgccggatcg gcgccgcggc
     4981 cctgcgcgac gagatcggcc ccggcgcgct ccgcccactc cgcgagctgc gggatcgcgg
     5041 ccgcacggta ggtgtcgctc gcggcgagcg cgacgagccg tccctgcccg acgagccggt
     5101 tcgccagctt cccgatcgtc gtcgtcttgc cggtcccgtt caccccgacg acgacgatca
     5161 ccgccgggcg tccctcgagg gagagaccgg ggtccccctc gaacatcccg gcgatctctt
     5221 cgatcacgag gcgctccggg tcctcgccgg gttcgaagcg ctcgcggaca cgggtgacga
     5281 tctcccgcgc ggcgcggggt ccggcgtcgg cagcgatcag cgcctcctcg agggcggtcc
     5341 agtcatcctc ggtgggcttc cccgatccga gccagcgccg cacgcggctc ccgagcccat
     5401 cggtctgcgg cgccgaggcg gcgtcggctc ggggcgccgg cgtcggctcg tgcgcgcgga
     5461 ggcggccgac gaccaacccc acgaagagca ggacgagcag tccggcgatg accacgatct
     5521 cgagcccgac gcccgacggc atggggcgat tatcgcgcgg cgggctcggg gacggcttcg
     5581 gcgacccgct gcgtgatgac ggtggtggcg ccggagcgtc ccatcgacac gccgtagagc
     5641 acgtcggccg cctccatcgt gcgcttctgg tgggtcacca cgaggacctg cgagtcacgc
     5701 gcgaagtctc ggatcagctg gatgaaccgg tggaggttga cgtcgtcgag cgccgcctcg
     5761 acctcgtcga ggaggtagaa cggcgacggc cgcgccttga agatcgcgaa caggaaggcg
     5821 agcgccgtga gagcgcgctc gccgccggac agcagggaga ggcgcttgac gcgcttgcga
     5881 cccgggcgcg cctcgatctc gatcccggtc gtcagcaggt cttcgccctc cgtgaggacg
     5941 atccggcctt cgcccccggg gaagacggtc tggaacatcc gctcgaactc ggtggcgacg
     6001 tcgtggaagg cggcgtcgaa gatctcgacg atgcggcggt cgacctcgcg gaccacctcc
     6061 aaaaggtcac gacgggccgc cttcacgtcg tcgatctcgc gctggaggaa gtcgtgccgc
     6121 tcctgcagcg ccgtgtactc ctcgttcgcc acgaggttga cgcggccgag gagcgtcagg
     6181 cggcgtgcga cgaggtccgc ccgcttctgg agcgcctcga cggtgtcgtc ctcgtccagg
     6241 gccgcgacgg cctcggcggg gtccttcccg tggccgtcgc gcaggagacg ctcggcctcg
     6301 ccgatccgcc gctcgaggtc gcggcgtgcc tggtcctcgt cctcgtactc gtcgcggagc
     6361 cgctgcagga gcgaggccgc ctcccgccac tcctcgttcg tcccgacc
//
LOCUS       DATLGG010000111         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_86404, whole genome shotgun sequence.
ACCESSION   DATLGG010000111 DATLGG010000000
VERSION     DATLGG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86404"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VM638_03150"
     CDS             complement(<1..654)
                     /locus_tag="VM638_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HVM11460.1"
                     /translation="MRIRELSLRNYRVFEEVDLELPARVIGIFGPNGGGKSTLVESIQ
                     FGLYGTTRTSKDMVRTDGVLTDCEVRLVFEHGGQQYEVRRAIKGKNHTAEAELLAGDL
                     QLAVGVREVTAEIGRLLHMDHQVFRASVFAEQKQLDAFSEIRKGERKAMVLRLLGIKP
                     VEDAIANARKEARDRTKDAERLSDSLPDVGEQESRLAAAKELREKGAKVADEAAADLE
                     "
     gene            complement(659..1057)
                     /gene="mscL"
                     /locus_tag="VM638_03155"
     CDS             complement(659..1057)
                     /gene="mscL"
                     /locus_tag="VM638_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVM11461.1"
                     /translation="MLKEFKAFAVRGNVVDLAVGIVIGAAFGAVVNSFVDDVVMNLIA
                     GIFGEPDFSDLVFGVGDGVVRYGAFVTALVNFFLVALALFFVVKALNRLRGTEREKPT
                     MRECPFCRTSIPKVASRCAACTSEVEPQAV"
     gene            complement(1074..2210)
                     /locus_tag="VM638_03160"
     CDS             complement(1074..2210)
                     /locus_tag="VM638_03160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HVM11462.1"
                     /translation="MARLVAFGDTHLGRTHLASLRDDQGRNLREEDFLRSFDWAIEET
                     LRQEPEGVLLLGDLFDHARPSYRVFTRVAVGLRRLSDAGVRGVAISGNHDTPRLRGTG
                     SPYAALEEFVEGFTFAWRMETVAADLDGLRVHAVPQTLDVPAFKEQLDAASASFDGDH
                     TNLLLAHVALTSLPARAYPDINELEVTEADLGSTFDLVVLGHYHVHQKASKHTWYAGA
                     TDSFSFADRPKGCGAKGLLVIDTDAGTAEHVPNPGERPLVTFGIDADGMGPAELVEAV
                     GRHNAELPGRAIVRVYLNEVDPAAFRQVPIDEFHAAMPQAAYVQVEPDFGAQALAVQG
                     APTIGTIEQEWSGFVETQDLAGLDRDRVRVMGERFLVEAKGEDR"
BASE COUNT          349 a          841 c          789 g          353 t
ORIGIN      
        1 ctcgagatcc gccgcagcct cgtcggcgac cttcgctccc ttctcgcgca gctccttcgc
       61 ggcggccagc ctcgactcct gctcaccgac gtcggggagg gagtcggaca gccgctcggc
      121 gtccttcgtc cggtcgcggg cctccttgcg cgcgttcgcg atcgcgtcct ccaccggctt
      181 gatcccgagg agccgcagga ccatcgcctt gcgttcgccc ttgcggatct cgctgaaggc
      241 gtcgagctgc ttctgctcgg cgaagacgga tgcccggaac acctggtggt ccatgtgcag
      301 gagccgtccg atctccgccg tcacctcccg cacgccgacg gcgagctgca ggtcaccggc
      361 gagcagctcg gcctcggcgg tgtggttctt gcccttgatc gcccggcgca cctcgtactg
      421 ctggcccccg tgctcgaaga ccaggcgaac ctcgcagtcg gtcagcacgc cgtcggtccg
      481 gaccatgtcc ttcgaggtgc gcgtcgttcc gtagagaccg aactggatcg attcgacgag
      541 cgtggacttg ccgccgccgt tggggccgaa gatcccgatc acgcgcgcgg gcagctcgag
      601 gtcgacctcc tcgaagaccc ggtagttgcg cagcgagagc tcgcggatcc tcacccgctc
      661 acaccgcctg cggctcgacc tcgctcgtgc acgccgcaca gcgcgatgcc accttcggga
      721 tgctcgtccg gcagaacggg cactcgcgca tcgtgggctt ctcccgctcg gtgccgcgga
      781 gacggttcag cgccttcacg acgaagaaga gggccagcgc gaccaggaag aagttcacga
      841 gcgcggtgac gaaggcgccg tagcgcacga cgccgtctcc cacgccgaac acgaggtcgg
      901 agaagtcggg ctccccgaag atgcccgcga tcaggttcat caccacgtcg tcgacgaagg
      961 agttcaccac cgcaccgaac gccgcgccga tgacgatgcc gacggcgagg tccacgacgt
     1021 tcccccggac cgcgaaggcc ttgaattcct tcagcacccc gaggccccct cgctcagcga
     1081 tcctcgccct tcgcctcgac caggaaccgc tcgcccatga cgcgcacgcg gtcgcggtcg
     1141 aggcccgcga ggtcctgcgt ctcgacgaac ccgctccatt cctgctcgat cgtcccgatc
     1201 gtcggagcgc cctgcaccgc gagcgcctgg gcgccgaagt ccggctccac ctgcacgtac
     1261 gccgcctgcg gcatcgctgc gtggaactcg tcgatcggga cctgccggaa ggcggcggga
     1321 tccacctcgt tgaggtagac gcgcacgatg gctcgccccg gaagctccgc gttgtgccgg
     1381 ccgaccgcct cgacgagctc cgccgggccc atcccgtcgg cgtcgatgcc gaaggtgacg
     1441 agcgggcgct ccccggggtt cggaacgtgc tcggcggtgc cggcgtcggt gtcgatgacg
     1501 aggagaccct tcgcgccgca gcccttcggc cggtccgcga acgagaacga gtcggtggcg
     1561 cccgcgtacc acgtgtgctt cgacgccttc tggtggacgt ggtaatggcc gagcacgacg
     1621 aggtcgaagg tggaaccgag atcggcctcc gtgacctcga gctcgttgat gtcggggtag
     1681 gcgcgggcgg ggagcgaggt gagcgcgacg tgcgcgagca ggaggttcgt gtggtcgccg
     1741 tcgaagctgg cgctcgccgc atcgagctgc tccttgaacg ccgggacgtc gagcgtctgc
     1801 gggacggcat gcacgcggag gccatcgagg tcggccgcca ccgtttccat ccgccacgcg
     1861 aacgtgaacc cctccacgaa ctcctcgagc gccgcgtagg gggagccggt cccacgcagg
     1921 cggggtgtgt cgtggttccc gctgatcgcc acgccccgga cgccggcatc cgagagacgc
     1981 cgcaggccga cggcgacccg cgtgaacacc cggtaggagg ggcgcgcatg gtcgaagagg
     2041 tccccgagca gcagcacccc ctcgggctcc tggcgcagcg tctcctcgat cgcccagtcg
     2101 aacgagcgga ggaagtcctc ctcccgcagg ttgcgcccct gatcgtcgcg cagtgaggcc
     2161 agatgcgtcc tgccgaggtg ggtgtcgccg aacgcgacga gacgagccat cagcggctcc
     2221 ggcggggccg aactacacgc atgtcattcc gatggtacca ctaggagccg agggcggctc
     2281 gcgtccgctc gccgagcgtc cggaggcgct cgaccggctc gcggctgtag ac
//
LOCUS       DATLGG010000112         2893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_3989, whole genome shotgun sequence.
ACCESSION   DATLGG010000112 DATLGG010000000
VERSION     DATLGG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3989"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2893
                     /locus_tag="VM638_03165"
     CDS             <1..>2893
                     /locus_tag="VM638_03165"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HVM11463.1"
                     /translation="AEPLTGVAGAIAKNMVASLEVPTATSVRSMPARLLEENRRIINR
                     WLGDRGKVSFTHLVGWAIVAAAERVPAMKRTFVEAEGRPHVVRHEAVNLGLAVDVERP
                     DGSRTLLVPNVKGANEMDFAAFWGAYEDVIRRTRGGKLTPDDFAGTTITLTNPGTIGT
                     ELSVPRLMQGQAAIIGVGRVAYPTELRGADPGVLANLGVGKTIGITSTYDHRVIQGAE
                     SGEFLAVMERLLLGEDGFYEDVFASLAIPHEPARWTEDRAALEPDSDERTRKQAAVLR
                     LVNMHRVRGHLIADLTPLGHDDVLAHPELDLGYHGLSVWDLDRPFLMDPEEEPRALRE
                     ILDRLRDAYCRTVGIEYMHIQDPEEKRWLRERIEGVSRDLDHDDQAHLLERLNAAEAF
                     EQFLHTRYVGHRRYGLEGAESLIPMLDFLLEEAIDSGVERVVMGMAHRGRLNVLANIV
                     GKSYQRIFREFEGDVDPESIHGSGDVQYHVGIAGKYQGRSGATVAVEVVSNPSHLEAV
                     NPVVQGIVRAAQYELGDRDRRRVLPVTMHGDAAFAGEGVVAETLGMSQLPGFTTGGTV
                     HVVINNQLGFTTPPSAGRSSTYATDVAKMVQAPILHVNGDDPEACVWAMRLALAYRQA
                     FRKDVVVDLLCYRRYGHNESDDPSYTQPLMYRKIEEKRSVRKLYMERLIDRGDISIEE
                     AEQALRDYRQRLETAFDEVQESHPPERGDLPSTRSTGVLPPVETGVQRSTLDRVLEAV
                     TSWPDGFTAHPKLARQLEKRRSMLAEDAVDWSMAEALAFGSLVLDGTRVRLSGQDSRR
                     GTFSQRHAVLVDHETGQEHYPLATLAEGKGAFAIFDSPLNEFATLGFEYGYSVESKSA
                     LVLWEAQFGDFFNEAQVIVDQFIVAGEDKWGQSSGLVMLLPHGFEGQGPEHSSGRIER
                     FLTLAAEDNIQVAVPTTPAQYFHLLRRQATRQVRKPLIVFTPKSFLRLPA"
BASE COUNT          465 a         1019 c         1012 g          397 t
ORIGIN      
        1 gggccgagcc gctcacgggc gtcgccggcg cgatcgcgaa gaacatggtc gccagcctcg
       61 aggtcccgac ggccacctcg gtccgctcga tgcccgcccg tctgctcgag gagaaccgcc
      121 ggatcatcaa ccgctggctc ggagaccgcg ggaaggtgag cttcacccac ctggtcggtt
      181 gggccatcgt tgccgcggcg gagcgggtgc ccgcgatgaa gcggacgttc gtcgaggccg
      241 aggggcggcc acacgtcgtc cgacacgagg cggtgaacct cgggctcgcg gtcgacgtgg
      301 agcgaccgga cggctcgcgg acgctcctcg tgccgaacgt gaagggggcg aacgagatgg
      361 acttcgccgc tttctggggc gcgtacgagg acgtgatccg gcggacgcgc ggcggcaagc
      421 tcacgccgga cgacttcgcc gggacgacga tcacgctcac gaaccccggc acgatcggga
      481 ccgagctctc cgtcccccgg ctgatgcagg gacaggccgc gatcatcggg gttggccgcg
      541 tcgcgtatcc gacggagctc cgaggcgcgg accccggggt cctcgcgaac ctcggagtcg
      601 gcaagacgat cggcatcacg agcacctacg accaccgcgt gatccagggc gccgagagcg
      661 gcgagttcct ggcggtgatg gagcggctgc tgctcggcga ggacgggttc tacgaggacg
      721 tcttcgcgag cctcgcgatc ccccacgagc cggcccgctg gacggaagac cgcgcggcgc
      781 tcgagcccga cagcgacgag cggacgcgga agcaggcggc ggtcctgcgc ctcgtcaaca
      841 tgcaccgggt ccgcggtcac ctgatcgccg acctcacgcc gctcggtcac gacgacgtgc
      901 tcgcccatcc cgagctcgac ctcggctacc acgggctctc ggtctgggac ctcgaccggc
      961 ccttcctcat ggatcccgag gaggagccgc gtgccctgcg cgagatcctc gaccgactgc
     1021 gcgacgccta ctgccgcacg gtcgggatcg agtacatgca catccaggac ccggaggaga
     1081 agcgctggct ccgcgagcgg atcgagggcg tctcacgaga cctcgaccac gacgaccagg
     1141 cgcacctcct ggagcgcctg aacgccgccg aggccttcga gcagttcctc cacacgcgct
     1201 acgtcggcca ccgccggtac gggctcgagg gcgcggagag cctgatcccc atgctcgact
     1261 tcctgctcga agaggcgatc gactccggcg tcgagcgggt cgtcatgggg atggcccacc
     1321 gcgggcggct gaacgtcctc gcgaacatcg tggggaagag ctaccagcgg atcttccgcg
     1381 agttcgaggg agacgtcgac cccgagtcga tccacgggtc cggcgacgtg cagtaccacg
     1441 tcgggatcgc cgggaagtac caggggcgtt cgggcgcgac ggtcgccgtg gaggtcgtgt
     1501 cgaacccgag ccacctcgag gcggtgaacc cggtcgtgca ggggatcgtc cgcgccgcgc
     1561 agtacgagct cggcgaccgc gaccggcgtc gtgtcctccc ggtcacgatg cacggcgacg
     1621 cggccttcgc gggagagggc gtcgtcgccg agacgctcgg gatgagccag cttcccgggt
     1681 tcacgaccgg gggcaccgtc cacgtcgtga tcaacaacca gctcggcttc acgacgccgc
     1741 cgtcggcggg gcgctcgagc acgtacgcga ccgacgtcgc gaagatggtg caggcgccga
     1801 tcctccacgt gaacggcgac gacccggagg cctgcgtctg ggccatgcgc ctcgcgctcg
     1861 cgtaccgcca agccttccgc aaggacgtcg tcgtcgacct cctctgctac cgccgctacg
     1921 gccacaacga gtccgacgac ccgagctaca cgcagccgct gatgtaccgg aagatcgagg
     1981 agaagcgctc ggtccggaag ctctacatgg agcggctgat cgaccgcggc gacatctcga
     2041 tcgaagaggc cgagcaggcc ctccgcgatt accgccagcg cctcgagacc gccttcgacg
     2101 aggtgcagga gagccacccg ccggagcgcg gcgacctccc gagcacccga tcgaccggcg
     2161 tgctgccccc ggtggagacc ggcgtccagc gctcgaccct cgaccgcgtg ctcgaggcgg
     2221 tgacgtcgtg gcccgacggg ttcacggcgc acccgaagct cgcgcgccag ctcgagaagc
     2281 gtcgctcgat gctggcggag gacgccgtcg actggtcgat ggccgaggcc ctcgccttcg
     2341 ggtcgctggt cctcgatggg acgcgcgtac ggctgtccgg acaggactcg cggcgcggaa
     2401 ccttcagcca gcggcatgcg gtactcgtcg accacgagac cgggcaggag cactacccgc
     2461 tcgcgacgct cgccgagggc aagggcgcgt tcgcgatctt cgactcgccg ctgaacgaat
     2521 tcgccacgct gggcttcgag tacggctact cggtggagtc caagagcgcg ctcgtgctgt
     2581 gggaggcgca gttcggggac ttcttcaacg aggcgcaggt gatcgtcgac cagttcatcg
     2641 tcgccggcga ggacaagtgg gggcagtcga gcggcctcgt gatgctgctc ccccacgggt
     2701 tcgaggggca gggccctgag cactccagcg ggcggatcga gcggttcctg accctagccg
     2761 ccgaggacaa catccaggtc gcggttccca cgacgcccgc gcagtacttc cacctgctcc
     2821 gccgccaggc gacccgccag gtgcgcaagc cgctgatcgt cttcacaccg aagtcgttcc
     2881 tccggctgcc ggc
//
LOCUS       DATLGG010000113         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_24220, whole genome shotgun sequence.
ACCESSION   DATLGG010000113 DATLGG010000000
VERSION     DATLGG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24220"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..930)
                     /locus_tag="VM638_03170"
     CDS             complement(175..930)
                     /locus_tag="VM638_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11464.1"
                     /translation="MGWLKDLFFVNEEKEAAREAGRATPKAPPARPKASSTVDAATDD
                     IIRRYAGGGGAASGERAPAVSTPAPDVSPPRSPSGEPPPDFGVAIPLKEGKVDFPEVF
                     RKAGIPADDMDRVEKALALLTQLPAETPHATKKQIVETSLKAFNIPIEEIIETAVAEI
                     QALHGCIDTGAKGTAALLSESEARIRKLEEEILEVRKVVEAAKSEQRALESAARAEGL
                     RIQRILEFFGEETVENVVKDPSAPLQHSRDAKK"
     gene            complement(945..2015)
                     /locus_tag="VM638_03175"
     CDS             complement(945..2015)
                     /locus_tag="VM638_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11465.1"
                     /translation="MTRLAPLLAASLLILLPSLVGCAPKTDTAAAPPQPTPTVLPAME
                     GEGHLRNLRQLTRGGENAEAYWSFDGRSLSMQSTRAGAKCDRIWRIDDVLSLPKLTQV
                     SSGKGRTTCAYFRNDGRILYSSTHEASEECPPVPDRSRGYVWPVYDSYDVYLEDGTPL
                     IAGKGYDAEATVCPVDGRIVFTSTRDGDLDLYVADADGKNVKRLTTEDGYDGGAFFNA
                     DCSKIVWRASRPQGAALQDYRKLLSEGLVRPSKLEIFVMNADGSGKRQVTSLGAAAFA
                     PSFLDSGRILFSSNHLDPKGRNFDIFLIGVDGTGLERITTYEGFDGFPLISPDGKRLA
                     FSSNRNGTEPGETNVFVADWVE"
     gene            complement(2012..>2520)
                     /locus_tag="VM638_03180"
     CDS             complement(2012..>2520)
                     /locus_tag="VM638_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVM11466.1"
                     /translation="IVPVRLGTDGKWFVDEIELYQEYKPVFPGWDEGAEKIHGIPRAH
                     LEEVGLDIKESMVALRDWAKGNSKKTRCVFVGHNAPFDWSFVAYYFAYVGMENPFGYN
                     ALDTKALAMGKLDLGWLETSKENLQGKLNLPPQDDSKVHNAVYDARYQALILAALLNL
                     PKRTDLPR"
BASE COUNT          352 a          851 c          887 g          430 t
ORIGIN      
        1 tggtctccga cgcgacgcgc gatcgctgcg gcgacgcctt cggctggcag gaggccggca
       61 ctgtcaccat ccgcgggaag acgggcgcgg tgacgacctg ggtgccgacg ctccgccgcg
      121 ccgcctgagt tgtggccacg aaattgtggg gacccacaat ttcgtggtca caacttactt
      181 cttcgcgtcg cgcgagtgct gcaggggcgc gctggggtcc ttcacgacgt tctcgaccgt
      241 ctcctcgccg aagaactcga ggatcctctg gatgcggagc ccctcggcgc gggcggcgga
      301 ctccagcgcg cgctgctcgg acttcgccgc ctcgacgacc ttgcgcacct cgaggatctc
      361 ctcctccagc ttgcggatgc gcgcctcgga ctcggagagg agcgccgcgg tccccttcgc
      421 gccggtgtcg atgcagccgt gcaacgcctg gatctcggca accgccgtct cgatgatctc
      481 ctcgatcggg atgttgaacg ccttcagcga ggtctcgacg atctgcttct tcgtcgcgtg
      541 cggcgtctcg gccggcagct gcgtgaggag ggcgagcgcc ttctcgacgc ggtccatgtc
      601 gtcggcgggg attccggcct tgcggaagac ctcggggaag tcgaccttcc cttccttcaa
      661 cgggatcgcg actccgaagt ccggcggcgg ctcccccgac gggctgcggg gcggcgagac
      721 gtccggcgcc ggcgtcgaca ccgccggggc tcgctccccg ctcgccgcgc ctccgccacc
      781 ggcgtagcgg cggatgatgt cgtcggtcgc ggcgtccacg gtcgaggagg ccttgggacg
      841 cgccggaggc gccttcggcg tcgcgcgccc ggcctccctc gccgcttcct tctcctcgtt
      901 gacgaagaac aggtccttga gccagcccat cggccgggat gctactactc gacccaatcg
      961 gcgacgaaca cgttcgtctc gcccggctcg gtgccgttgc ggttcgagga gaacgcgagg
     1021 cgcttcccgt cgggcgagat gagcgggaac ccgtcgaagc cttcgtaggt ggtgatccgc
     1081 tcgagccccg tgccgtcgac cccgatgagg aagatgtcga agttcctccc cttcggatcg
     1141 aggtggttcg acgagaagag gatccgcccg gagtcgagga acgacggagc gaacgccgcc
     1201 gctccgagcg acgtcacctg gcgcttgccg gatccgtcgg cgttcatgac gaagatctcg
     1261 agcttcgacg gccgcacgag cccctcggag agcagcttgc ggtagtcctg cagcgccgcg
     1321 ccttgcggcc ggctcgcgcg ccacacgatc ttcgagcagt cggcgttgaa gaacgcgccg
     1381 ccgtcgtagc cgtcctccgt cgtgaggcgc ttcacgttct ttccgtccgc gtccgccacg
     1441 tagaggtcga ggtcgccgtc gcgcgtcgag gtgaagacga tccggccgtc gacggggcag
     1501 accgtcgcct cggcgtcgta ccccttcccg gcgatcagcg gcgtgccgtc ctccaggtag
     1561 acgtcgtagg agtcgtagac cggccagacg tagccacgcg agcggtccgg aaccggcggg
     1621 cactcctccg acgcctcgtg cgtggacgag tagaggatgc gcccgtcatt ccggaagtag
     1681 gcgcaggtcg tccgcccctt gccgctcgag acctgcgtga gcttcgggag ggagaggacg
     1741 tcgtcgatgc gccagatccg gtcgcacttc gcgccggcgc gcgtggactg catcgagagc
     1801 gatcggccgt cgaaggacca gtaggcctcg gcgttctcgc cgccgcgcgt gagctgccgg
     1861 aggttccgga gatggccctc cccctccatc gcgggcagca cggtgggggt cggctgcggc
     1921 ggcgccgccg ccgtgtcggt cttcggcgcg cagccgacga gggaagggag caggatcagg
     1981 agggaggcgg cgaggagcgg agcgagtcgg gtcatctcgg gaggtcggtg cgcttcggca
     2041 ggttcaggag ggcggcgagg atgagcgcct ggtagcgggc gtcgtagacg gcgttgtgca
     2101 ccttcgagtc gtcctgcggc ggcaggttca gcttcccctg caggttctcc ttcgacgtct
     2161 cgagccaccc gaggtcgagc ttgcccatcg cgagcgcctt ggtgtcgagg gcgttgtagc
     2221 cgaaggggtt ctccatcccg acgtaggcga agtagtaggc gacgaagctc cagtcgaacg
     2281 gcgcgttgtg gccgacgaag acgcaccgcg tcttcttgga gttccccttc gcccaatcgc
     2341 ggagagcgac catcgactcc ttgatgtcca ggcccacctc ctcgaggtgc gcgcgcggga
     2401 tgccgtggat cttctccgcg ccttcgtccc agccggggaa gaccggcttg tactcctggt
     2461 agagctcgat ctcgtcgacg aaccacttgc cgtcggtgcc gagccggacc gggacgatgc
//
LOCUS       DATLGG010000114        10615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_24413, whole genome shotgun sequence.
ACCESSION   DATLGG010000114 DATLGG010000000
VERSION     DATLGG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24413"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VM638_03185"
     CDS             <1..186
                     /locus_tag="VM638_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11467.1"
                     /translation="GSVTVDAGRSRVVELTPEEGGEPVAVLVEGPDASLVVAGAGVTE
                     AGYSLALGVRLDPLRVP"
     gene            206..1258
                     /locus_tag="VM638_03190"
     CDS             206..1258
                     /locus_tag="VM638_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HVM11468.1"
                     /translation="MARGRHTRRRRGRILLSLTVALSLLGGGAAVAAYRYDQATVGRV
                     MPGVSVEGVDLSEMTRPQALSALSEVADRELDRVIEVQAAGKTWEVTPAELGGTADVE
                     AKVDAALALSDGFSWPSRVFRRMLDRPLEREADLSYSFDQAQVERFVDVIAKQVKVSP
                     RDPEIAIGEDGDLVLAKAKRGRRLQVKAAREAIASALEDGTSSVELPMRKVAPKEKEL
                     GQTIVVRISENKLYLYRGLELRKTYRVATGAPGYPTPKGTWTIWHKAKNPTWTNPAPD
                     GWGANMPKTIPGGPNNPLGTRALYLDAPGIRIHGTSNVGSIGTYASHGCIRMTMPEVE
                     ELYEMIEIGTKVLIID"
     gene            1328..1564
                     /locus_tag="VM638_03195"
     CDS             1328..1564
                     /locus_tag="VM638_03195"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HVM11469.1"
                     /translation="MRPCAKQGCGERAEASVGVRYADRMVVIGALPPEPDPNLIELCA
                     RHTDSLTPPYGWNRLDQRPASATPRVELPAELTA"
     gene            1698..1886
                     /locus_tag="VM638_03200"
     CDS             1698..1886
                     /locus_tag="VM638_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11470.1"
                     /translation="MSDIKKVTRCPACTSDDIVSIEMDMEGVGVHFQACHKCENRWWE
                     RDGERIPLESILSLVPRR"
     gene            1933..3265
                     /locus_tag="VM638_03205"
                     /pseudo
     CDS             1933..3265
                     /locus_tag="VM638_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
     gene            3262..3435
                     /locus_tag="VM638_03210"
     CDS             3262..3435
                     /locus_tag="VM638_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HVM11471.1"
                     /translation="MTIDKELLEILICPNCRGEIEYRPDDELIVCVGRCRYRYPVRDG
                     IPVMLVEEAIKPA"
     gene            3432..4469
                     /locus_tag="VM638_03215"
     CDS             3432..4469
                     /locus_tag="VM638_03215"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVM11472.1"
                     /translation="MIPLDDEAALRAGDPSGLLDAFATLPRQLREGYAVGLEAAVPSP
                     ADPRAVVLCGVGGSAAAADVVEAALAERADRPVVVLRDDRVPTWVDDRTLVVCLSYSG
                     TTAETIAAAEDASRRRATPIVVASGGPLAREAGERAVLVPPSAPAPRAALGWLTGALL
                     GVLEAAGIGGPDGRAAVASEELERTVSALTPGVPMAENAAKRVADRLGDRVPVVWAPV
                     PNLLPAALRWKAAFNENAEIPAFASFLPELAHHEVVGWTKQWSDRFAVVGLRAPDEEG
                     PAGDRTRAALDLIAEAGIEHEEIHVGGRDLAALLGLIAVGDFASTYHALMRGIDPAPI
                     EAIRRLKDRLR"
     gene            4469..5296
                     /locus_tag="VM638_03220"
     CDS             4469..5296
                     /locus_tag="VM638_03220"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HVM11473.1"
                     /translation="MGPGDDLADRGAAVVRGLVDVQPRLAVILGSGLGPAAVALEPEL
                     EIAYPDLPGFPEPTVPGHAGRLRVGTLSGVPAIAFLGRIHAYEGHPMPLVTLPVRLAA
                     ALGVRTLVVTAAAGGLDPALRPGSLVIGEDHLNFLGESPLRGWRGADGTPAFVDLSEA
                     YPRDLSEMAHRAAEAEGMTPVGGVYASMPGPAYETPAEIEFLRRSGATVVGMSVVPEV
                     TAAVALGIRCLGLYCVTNTVGAGVSHTEVQEVATAFSSDLGRLLARLAPTLERKEQG"
     gene            5293..6540
                     /gene="ahcY"
                     /locus_tag="VM638_03225"
     CDS             5293..6540
                     /gene="ahcY"
                     /locus_tag="VM638_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVM11474.1"
                     /translation="MSDVRDAGLADRGRERIEWAAREMKVLELIRERFEKERPLDGLR
                     LAACLHVTTETANLVRTLAAGGAEVALCASNPLSTQDDVAAALADEVHTFAIRGIDDE
                     GYYRHINAALDLRPSITMDDGADLVNTLHQKRTDQLPEVVGGTEETTTGVIRLRAMAE
                     DGVLAYPVISVNDAKTKHLFDNRFGTGQSTIDAIMRATNLLLAGRIVVVMGYGMCGRG
                     VASRARGFGAQVVITEVDPVSALEAAMEGYRVLPGREAARIGDIFVTVTGDTGVIRRE
                     HIDLMKDGAVLANSGHFDVEIDKSALREAAGEPRRLREHVDEYRMPDGRRIHLLGEGR
                     LVNLAAAEGHPAAVMDMSFANQALSVEWVARNADSLERRVYPVPEEIDAEVARLKLHS
                     MGIEIDELTPEQESYLRSWEHGT"
     gene            6522..7217
                     /locus_tag="VM638_03230"
     CDS             6522..7217
                     /locus_tag="VM638_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11475.1"
                     /translation="MGARDLSGPHPVDVGVVFDRFPASIRGAVLVRGADPDPHQITVR
                     AAYLVEAAEPGRAAAPVEVDQTTVELAPRAEVFFPFDVPVTGLEAGWYGVVAEVVVDG
                     GIERRSEEPGKLFPVAWPGGTVRRGSVAVGRSIPVPTHGDVVVERLEMKGDRAIVQWR
                     LPPGTGEEDVSVLADGRPLPLLDRSERHGVRSATVYPVLRAHRTLAVRFERIRGADGS
                     WIRGPWDLEIPLP"
     gene            7247..7978
                     /locus_tag="VM638_03235"
     CDS             7247..7978
                     /locus_tag="VM638_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HVM11476.1"
                     /translation="MLDLLFPPRCASCGAAGRSVCPVCARRVPVVTPPLCARCGRPSE
                     DPLSACAECPPPVISVVRTPFLYDGPVADLVRALKFRGWAWAARALGGAMAAVWEERV
                     DVVTWVPLSRARRRERGFDQAERLARVVARRLDRPLEPLLRRVADTPAQARLSGPDRR
                     RALRDAFVARRRSARSVLLVDDVLTTGATAAACARALREAGAAEVAVLAAARSLGRGV
                     PPRCLTVESDGLSPGSVVAREAFSR"
     gene            8217..8597
                     /gene="raiA"
                     /locus_tag="VM638_03240"
     CDS             8217..8597
                     /gene="raiA"
                     /locus_tag="VM638_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVM11477.1"
                     /translation="MDLVVKARGGKVTQHARERIEHKLRKLSRLDSKIQRLEIEVIQE
                     STPRVDGGHRVEAAAVTARRTFRATATAHDVEAAVERAADRLQRQVSEEHGRRRARLI
                     GGAHRVKSADRAKGGATEPETPEA"
     gene            8607..9302
                     /locus_tag="VM638_03245"
     CDS             8607..9302
                     /locus_tag="VM638_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM11478.1"
                     /translation="MAKKQTPAKPSREPLRVLIVDDHGLFRKGLQMVLEQEEDLTVVG
                     EAADGHEAVEKSQELMPDVVLMDVRMPKRTGIEATKQIKDALPHAKILMLTISDDEAD
                     LYDAIKAGASGYLLKEINTEEVAEAIRSVWQGQSRISPSMAAKLLNEFQAMSKRAEER
                     AQVPSPKLTEREMEVLRLVARGLNNRDIAKELYISENTVKNHIRNILEKLQLHSRMEA
                     VVHAVREGLLEIK"
     gene            9351..>10615
                     /locus_tag="VM638_03250"
     CDS             9351..>10615
                     /locus_tag="VM638_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HVM11479.1"
                     /translation="MFQKLSQYGETRKVKRLQDHVEAVNAFEPEIEQLTDAELRERTE
                     RFRERIADTREGLDEEEAKRATAEILDEILPEAFACVREAAKRTIGQRHYDVQLMGGV
                     VLHHGDIAEMKTGEGKTLVSTLPTYLNSLAGGGVHVVTVNDYLAKRDSEWMSPIYRLL
                     GVSVGLIQGQMAPEQRRPAYGSDITYGTNNEFGFDYLRDNMAMSKEEMVQRGHYFAIV
                     DEVDSILIDEARTPLIISGPASDSTKQYSTFARLAPRLKKDIDYEVDEAKRTVAVTEA
                     GVSRVEEYLGVDNLYEHVHSGLVHHLQNALRAKELYRKDVDYIVDGGEVKIVDEFTGR
                     VLEGRRYSEGLHQAIEAKEGVRVQQENQTLATITIQNYFRMYDKLGGMTGTAKTQARE
                     FEEVYKLGVVTIPTNRPMVRRDEQDLIYKT"
BASE COUNT         1574 a         3707 c         3873 g         1461 t
ORIGIN      
        1 gggagcgtca cggtggacgc gggacgcagc cgggtcgtcg agctgacgcc ggaggagggc
       61 ggggagcccg tcgccgtgct cgtcgaggga cccgacgcga gcctcgtcgt ggccggtgcc
      121 ggggtgaccg aggcgggcta ctccctcgcc ctcggggtcc gcctcgaccc gcttcgggtg
      181 ccttgacggg gtatcatcag gggtcatggc acggggtcga cacacacgcc gccggcgcgg
      241 ccggatcctc ctgtcgctga ccgtcgcgct ctcgctgctc ggcgggggag cggccgtcgc
      301 ggcgtaccgc tacgaccagg cgacggtcgg acgcgtgatg ccgggcgtct cggtcgaggg
      361 ggtcgacctc agcgagatga cccgtccgca agcgctgagc gccctcagcg aggtggcgga
      421 tcgcgagctg gatcgcgtca tcgaggtcca ggccgccggc aagacgtggg aggtgacgcc
      481 cgccgagctt ggcggaaccg ccgacgtcga ggcgaaggtg gacgcggcgc tcgcgctctc
      541 ggatgggttc agctggccct cccgcgtgtt ccggcgcatg ctcgaccggc cgctcgagcg
      601 tgaggcggac ctgagctact ccttcgatca ggcgcaggtc gagcggttcg tagacgtgat
      661 cgcgaagcag gtcaaggtct ccccacgcga tcccgagatc gcgatcggcg aggacggcga
      721 tctggtgctg gcgaaggcga agcgcggtcg ccgcctccag gtgaaggccg ctcgcgaggc
      781 catcgcgtcc gccctcgagg acgggacctc ctcggtcgag ctgccgatgc gcaaggtcgc
      841 cccgaaggag aaggagctcg gccagacgat cgtcgttcgc atctccgaga acaagctgta
      901 cctgtaccgc ggcctcgagc tccgcaagac ctaccgcgtc gcgaccgggg cccccggcta
      961 cccgacgccg aaggggacgt ggacgatctg gcacaaggcc aagaacccga cgtggaccaa
     1021 cccggccccc gacgggtggg gcgccaacat gccgaagacg atccccggcg gcccgaacaa
     1081 ccccctcggg acccgggcgc tgtacctcga cgccccgggc atccggatcc acggcacctc
     1141 gaacgtcggc tcgatcggca cctacgcctc gcacggctgc atccggatga cgatgcccga
     1201 ggtcgaggag ctctacgaga tgatcgagat cgggacgaag gtcctgatca tcgactgacg
     1261 ttctcgcccg aacccttcct cgcccgaacc cttgcggatc acatcgatgt gatttacgct
     1321 ggctctcgtg cgaccgtgcg cgaagcaggg atgcggggag cgggcggagg cgagcgtcgg
     1381 ggtccgctac gccgaccgga tggtcgtgat cggagcgttg cctccggagc ccgacccgaa
     1441 cctcatcgag ctctgcgccc gccacacgga cagcctgacg cccccctacg ggtggaatcg
     1501 cctcgaccag cgtccggcct cggcgacgcc gcgggtcgag ctgccggccg agctgaccgc
     1561 ctgagctccg ctggagccgg ccggtcgcgt gctcgcgcat ggcccgagat gactacctcg
     1621 gcgtggcccg tcgcgtcgcg gcgaaatgct cccgtcgggc tatggccgat ccgcgcctgg
     1681 agcgcgaaca tcgtcccatg tcggatatca agaaggtcac ccgctgcccc gcctgcacct
     1741 ccgatgacat cgtctcgatc gagatggaca tggagggcgt cggcgtccac ttccaggcgt
     1801 gccacaagtg cgagaaccgc tggtgggagc gcgacggcga gcggatcccg ctcgagtcga
     1861 tcctctcgct cgttccgcga cgctgagcga aggctccacc ggacgctggg atagaatccc
     1921 gcctcggtgg ccctggaacg catcttcaag gcgtacgacg ttcgtggcgt cgtccccgat
     1981 gagctgaacg aggaggtcgc gcgccgcatc ggcgcggcgt tcgcggactt cgtcgcggcg
     2041 ccctccgtcg tcgtcggccg cgactgccgc ccgtcgtccc cctcgctcgt cgaggccttc
     2101 gtcgacggcg tgaccggtcg cgggaccgac gtcgtggaca tcggcctcgc cacgaccgac
     2161 atgctttacc tcgcgtccgg gcggctcgac gtgccgggag cgatgttcac cgcgagccac
     2221 aacccgcctc gatacaacgg tttgaagctg tgccgcgcgg gcgcggcgcc gatcgggcag
     2281 gactcgggtt tgctcgagct ccgcgacgcc gccgccgccg accccgcacc ggcggcggca
     2341 cgaggcaccg tcagccggcg cgacatgctc gaggagtacg tcgaccacgt cgtctccttc
     2401 atcgacccca acgcgatggc tccgctcgtg gtggtcgccg acgcggcgaa cgggatggcc
     2461 gggctcgtgc tgcccccgat cttcgaacgg ctcccggcga agctcgtggg gctctacctg
     2521 gacctcgacg ggtccttccc gaaccacccg gccgatccga tccagcccga gaaccaggcg
     2581 gatcttcgcc gcgccgtgcg ggagaacggc gccgacatcg gcctggcgtt cgacggcgac
     2641 gcggaccgcg tcttcctcgt cgatgagcgg gccgaagggg tctcgggctc gctcgtcacg
     2701 gcgctcgtcg cggaagcgat gctcgagcgc gagccgggcg cgaaggtgat ccacaacctg
     2761 atctgctcgt gggtcgtgcc ggaggtcgtg cgggagcgcg gcggcgagcc gatccgaacc
     2821 cgcgtcgggc actcgttcat caagaaggtg atggcggaga ccggggcgat cttcggcggc
     2881 gagcactccc ggccactact acttccgcga ccactaccgt gccgacagcg ggctcatcgc
     2941 ggcgctcgtc gtcctcgacc ggctctcgcg cgcgggggtg ccgctgtcgg agctgctggc
     3001 gccgtaccag cgctaccacg cgtcggggga gatcaacacc gaggtcgccg atcagcaggc
     3061 ggcggtcgag cgcgtcacgg cgagcttcgc cgatggacgg gtggaccgcg tcgacgggct
     3121 gacggtggag tacgacgact ggtggttcaa cgttcgcccg tcgaacaccg agccgctgct
     3181 ccgtctcaac gtggaggcga agacggccga gctgctcgcg gagaagaccg aagccgtgct
     3241 ggacgtgatc cggagggagc cgtgacgatc gacaaggaac tgctcgagat cctgatctgc
     3301 cccaactgcc ggggcgagat cgagtaccgt cccgacgacg agctcatcgt gtgcgtcggg
     3361 cggtgccggt accgctaccc cgtgcgcgac gggatcccgg tgatgctcgt cgaggaagcg
     3421 atcaagcccg cttgatcccg ctcgacgacg aggccgcgct gcgcgccggc gacccgtcgg
     3481 ggctcctcga cgccttcgcg acgctcccgc ggcagctgcg ggaggggtac gcggtcggcc
     3541 tcgaagcggc cgtcccttcc cctgccgatc cgcgtgcggt cgtcctctgc ggcgtcggcg
     3601 gatccgccgc ggccgccgac gtggtcgaag cggccctggc ggagcgcgcc gaccggccgg
     3661 tcgtcgtgct ccgcgacgac cgcgtcccca cctgggtcga cgaccgcacg ctcgtcgtct
     3721 gcctgtcgta cagcgggacg acggcggaga cgatcgccgc tgcggaggac gcctcgcgcc
     3781 gccgggcgac cccgatcgtg gtcgcctccg gtggtccgct cgcccgcgag gcgggggaac
     3841 gcgccgtgct cgttccgccc tcggcgcccg ctccgcgtgc ggcgctcggg tggctgacgg
     3901 gcgccctgct cggcgtcctc gaggcggccg ggatcggcgg tcccgacggg cgcgccgcgg
     3961 tcgcatccga agagctggag cggaccgtct cggccctgac gccgggcgtg ccgatggccg
     4021 agaacgcggc gaagcgcgtc gccgaccgcc tcggcgaccg cgtcccggtg gtctgggcgc
     4081 ccgttccgaa cctcctgccg gcggcgctcc ggtggaaggc agccttcaac gagaacgccg
     4141 agatccccgc cttcgcctcg ttcctgccgg agctggccca ccacgaggtc gtcgggtgga
     4201 cgaagcagtg gtccgaccgc ttcgcggtgg tcggcctgcg cgcgccggac gaggagggtc
     4261 ccgccggcga ccggacgcgg gccgcgctcg acctcatcgc cgaggccggc atcgagcacg
     4321 aggagatcca cgtcggaggc cgggacctcg ccgcgctgct cgggctgatc gcggtcggcg
     4381 acttcgcgag cacctaccac gcgctgatgc gcgggatcga cccggcgccg atcgaggcga
     4441 tccggcggct gaaggaccgg ctccgctgat gggccccggc gacgacctcg ccgatcgggg
     4501 cgccgccgtc gtccgcgggc tcgtcgacgt ccagccgcgg ctcgcggtca tcctcgggtc
     4561 cgggctgggc cccgctgccg tggccctcga gcccgagctg gagatcgcct accccgacct
     4621 gccgggtttc cccgagccga ccgtgccggg ccacgccggg cgcctgcggg tcgggacgct
     4681 gtcgggggtg ccggcgatcg cgttcctggg gcggatccac gcctacgagg gacacccgat
     4741 gccgctcgtc accctgcccg tccggctcgc ggccgcgctc ggcgtccgga cgctggtcgt
     4801 caccgccgcg gcgggcggtc tcgaccccgc gctgcggcca ggctccctcg tgatcggcga
     4861 ggaccacctc aacttcctcg gcgagagccc gctccgcgga tggcggggag ccgacgggac
     4921 cccggcgttc gtggacctga gcgaggcgta cccgcgcgac ctgagcgaga tggcgcaccg
     4981 ggccgcggag gccgagggga tgacgcccgt cgggggcgtg tacgcttcga tgccgggccc
     5041 cgcgtacgag acgccggcgg agatcgagtt cctgcgccgg agcggggcga cggtcgtggg
     5101 catgtcggtg gtcccggagg tgaccgcagc ggtcgcgctg gggatccgct gcctggggct
     5161 ctactgcgtg acgaacacgg tgggtgcggg cgtcagccac accgaggtgc aggaggtcgc
     5221 gacggccttc tcgtccgacc tcggacggct cctcgcgcgc ctcgcgccca cgctcgagcg
     5281 gaaggagcag ggatgagcga tgtgagggat gccggtctcg ccgatcgcgg gcgtgagcgg
     5341 atcgagtggg cggcacggga gatgaaggtc ctcgagctga tccgggagcg gttcgagaag
     5401 gagcggccgc tcgacggatt gcggctggcg gcctgcctcc acgtgacgac ggagaccgcg
     5461 aacctcgtgc ggacgctggc agcgggtggg gcggaggtcg cgctctgcgc gtcgaacccg
     5521 ctctcgaccc aggacgacgt cgcggccgcg ctcgcggacg aggtccacac gttcgcgatc
     5581 cgggggatcg acgacgaggg ctactaccgc cacatcaacg cggcgctcga cctccggccc
     5641 tccatcacga tggacgacgg cgccgacctc gtgaacaccc ttcaccagaa gcggacggat
     5701 cagctccccg aggtggtcgg cggcaccgag gagacgacga ccggcgtgat ccggctccgg
     5761 gcgatggccg aggacggcgt cctcgcctac cccgtcatct cggtgaacga cgcgaagacg
     5821 aagcacctgt tcgacaaccg cttcgggacc ggccaatcga cgatcgacgc gatcatgcgg
     5881 gcgacgaacc tcctgctcgc cgggcgcatc gtcgtcgtca tgggctacgg gatgtgcggc
     5941 cgaggggtcg cctcccgcgc gcgcggcttc ggcgcgcagg tcgtcatcac cgaggtcgac
     6001 ccggtctcgg cgctcgaggc cgcgatggag ggctaccggg tgctgccggg gcgcgaggcc
     6061 gcccgcatcg gtgacatctt cgtcacggtg accggcgaca ccggcgtgat ccgtcgcgag
     6121 cacatcgatc tcatgaagga tggggcggtt ctcgcgaact ccgggcactt cgatgtggag
     6181 atcgacaagt cggcgctccg cgaggccgcg ggcgagcccc gcaggctccg cgagcacgtc
     6241 gacgagtacc gcatgccgga cgggcggagg atccaccttc tcggcgaggg acgcctcgtg
     6301 aacctcgcgg ccgccgaggg gcatccggcc gccgtgatgg acatgtcgtt cgccaaccag
     6361 gcgctgtcgg tggaatgggt cgcgcgcaac gccgactcgc tcgagcgacg cgtctacccg
     6421 gtgcccgagg agatcgacgc ggaggtggcg cgcctgaagc tgcattcgat gggcatcgag
     6481 atcgacgagc tgacgcccga gcaggagtcg tacctgcggt cgtgggagca cgggacctga
     6541 gcggacctca tcccgtcgac gtcggggtgg tcttcgaccg cttcccggcg tcgatccgtg
     6601 gcgccgtgct cgttcggggc gccgatccgg acccgcatca gatcacggtg cgtgccgcgt
     6661 acctcgtcga ggcggccgag cccggtcgcg cggccgcacc ggtcgaggtc gaccagacga
     6721 cggtcgagct cgcgccgagg gcggaggtct tcttcccctt cgacgtgccc gtcacggggc
     6781 tcgaggcggg ctggtacggg gtcgtcgccg aagtggtcgt cgacggcggg atcgagcgcc
     6841 ggagcgagga gccagggaag ctcttccccg tcgcctggcc cgggggcacc gtccggcggg
     6901 ggagcgtcgc ggtcggccgc tccatcccgg ttcccaccca cggggacgtc gtcgtcgagc
     6961 gcctcgagat gaagggcgat cgcgcgatcg tgcagtggag gctcccgccg ggcaccggcg
     7021 aggaggacgt gagcgtgctg gccgacggcc gccccctccc actcctcgac cggtccgagc
     7081 gacacggggt gcgcagcgcc acggtctacc ccgtcctgcg cgcgcaccgg acgctggcgg
     7141 tgcggttcga gcggatccgc ggcgccgatg gctcctggat ccgcggtccc tgggacctgg
     7201 agatccccct gccgtaggtg tcggtgcccg gcggtaggct cgccggatgc tcgacctgct
     7261 gttcccgccc cgctgcgcgt cctgcggggc cgcgggacgt tccgtctgcc ccgtctgcgc
     7321 gcggcgcgtc ccggtcgtga cgccgccgct ctgcgctcgg tgcgggcggc cctccgagga
     7381 cccgctgtcg gcctgcgccg agtgtccgcc cccggtgatc tccgtcgtcc ggacgccgtt
     7441 cctgtacgac ggcccggtcg ccgacctcgt ccgggcgctg aagttccgcg gatgggcctg
     7501 ggcggcacgc gccctcggcg gggcgatggc ggccgtgtgg gaggagcggg tcgatgtcgt
     7561 gacgtgggtc ccgttgtcga gggcgcgtcg gcgcgagcgc gggttcgacc aggcggagcg
     7621 cctggcgcgg gtcgtcgcgc ggcgcctcga ccggccgctc gagccgctgc tccgtcgcgt
     7681 cgccgatacc ccggcgcagg cgcgactgag cggccccgac cgacgccggg ccctgcgcga
     7741 cgccttcgtc gccagacgcc gctccgcccg gtcggtgctc ctcgtggacg acgtgctgac
     7801 gacgggggcg acggcggcgg cgtgcgcgcg ggcgctgcgc gaggcgggag ccgccgaggt
     7861 cgccgtcctc gccgcggccc gctccctcgg ccggggggtc cccccccggt gcctcacggt
     7921 agaatcggat gggctctcgc ctgggtctgt ggttgcccgg gaggcgttct cccggtagtc
     7981 gatgccagcc gtaggcgaaa cgacccacgt aaggcgaccg ttggtcgccg agcatggtgc
     8041 ggcttagatg taagtcctgc cccggtgctc cgggagaagg tgctaagccg tccgcgagga
     8101 cggtgacccc ctcggcaggc gggtgtgggg gcaaagacca ggtcagccgg gcgagggtcc
     8161 caccggccgg gccggtcccg agccggagcg tgggcgggcg acaggaagga gccggcatgg
     8221 acctcgtcgt gaaggcccgt ggggggaagg tcactcagca cgcccgggag cgcatcgagc
     8281 acaagctccg gaagctctcc cgcctcgact cgaagatcca gcgtctcgag atcgaggtga
     8341 tccaggagtc cacgccgcgg gtcgacgggg ggcaccgcgt cgaggccgcg gcggtgacgg
     8401 ctcgccggac gttccgagcc accgcgaccg ctcacgatgt cgaagcggcc gtcgagcgcg
     8461 ccgccgaccg cctccagcgc caggtctccg aggagcacgg acggcgccgg gctcggctga
     8521 tcggcggagc ccatcgggta aaatcagccg accgtgccaa gggcggggcg accgagcccg
     8581 aaacgcccga ggcctgagga gcgccattgg ctaagaagca gacgcccgcg aagccgagcc
     8641 gggagcccct gcgcgtcctg atcgtcgacg accacgggct cttccggaag ggcctgcaga
     8701 tggtcctcga gcaggaggag gacctgacgg tcgtcggcga ggcggccgac gggcacgagg
     8761 cggtcgagaa gtcccaggag ctgatgcccg acgtcgtcct gatggacgtg cggatgccga
     8821 agcggaccgg gatcgaggcg acgaagcaga tcaaggacgc gctcccgcac gcgaagatcc
     8881 tcatgctcac gatcagcgac gacgaggcgg acctctacga cgcgatcaag gcgggcgcga
     8941 gcggctatct gctcaaggag atcaacaccg aggaggtcgc cgaggcgatc cgcagcgtct
     9001 ggcaggggca gtcgcggatc tcaccgtcga tggccgcgaa gctcctgaac gagttccagg
     9061 cgatgagcaa gcgggccgag gagcgggccc aggttccgag cccgaagctc accgagcgcg
     9121 agatggaggt cctccgcctc gtcgcgcgcg gcctcaacaa ccgcgacatc gccaaggagc
     9181 tctacatctc ggagaacacc gtcaagaacc acatccgcaa catcctcgag aagctccagc
     9241 tccactcgcg gatggaggcc gtcgtccacg cggtccggga gggcctgctg gagatcaagt
     9301 agcgggaccc tcctggtacc atcggtcgga gccatcccga ggagcgaagc gtgttccaga
     9361 agctcagcca gtacggcgag acccgcaagg tcaagcgcct ccaggaccac gtcgaggcgg
     9421 tcaacgcgtt cgagccggag atcgagcagc tcacggatgc cgagctgcgc gagcggaccg
     9481 agcgcttccg ggagcggatc gccgacacgc gcgaggggct cgacgaggag gaggcgaagc
     9541 gggccaccgc cgagatcctc gacgagatcc tccccgaggc cttcgcctgc gtccgcgagg
     9601 cggcgaagcg gacgatcggc cagcgccact acgacgtgca gctcatgggc ggggtcgtcc
     9661 tgcaccacgg cgacatcgcc gagatgaaga ccggtgaggg caagaccctc gtttcgacgc
     9721 tgcccaccta cctgaactcg ctcgcgggcg ggggcgtgca cgtcgtcacc gtcaacgact
     9781 acctcgcgaa gcgcgactcc gagtggatgt ccccgatcta ccggctcctc ggcgtctccg
     9841 tcggtctcat ccagggtcag atggcccccg agcagcggcg gccggcgtac ggctcggaca
     9901 tcacctacgg cacgaacaac gagttcggct tcgactacct ccgcgacaac atggcgatgt
     9961 cgaaggagga gatggtccaa cgcggccact acttcgcgat cgtcgacgag gtcgactcga
    10021 tcctgatcga cgaggcgagg acgccgctca tcatcagcgg cccggcctcg gattccacga
    10081 agcagtactc gaccttcgcg cggctcgccc cgcgcctcaa gaaggacatc gactacgagg
    10141 tcgatgaggc gaagcggacc gtggcggtca cggaggcggg agtctcgcgc gtcgaggagt
    10201 acctcggcgt cgacaacctc tacgagcacg tgcactccgg cctcgttcat cacctccaga
    10261 acgcgctccg agcgaaggag ctgtaccgga aggacgtcga ctacatcgtc gacgggggcg
    10321 aggtgaagat cgtcgacgag ttcaccggcc gcgtgctcga gggccgtcgc tactccgagg
    10381 gcctgcacca ggcgatcgag gcgaaggagg gggtgcgcgt ccagcaggag aaccagacgc
    10441 tcgccacgat cacgatccag aactacttcc ggatgtacga caagctcggc ggcatgaccg
    10501 ggaccgcgaa gacgcaggcg cgcgagttcg aggaggtcta caagctcggc gtcgtgacga
    10561 tcccgaccaa ccgaccgatg gtccgtcgcg acgagcaaga cctcatctac aagac
//
LOCUS       DATLGG010000115         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_148112, whole genome shotgun sequence.
ACCESSION   DATLGG010000115 DATLGG010000000
VERSION     DATLGG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148112"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="tilS"
                     /locus_tag="VM638_03255"
     CDS             <1..478
                     /gene="tilS"
                     /locus_tag="VM638_03255"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVM11480.1"
                     /translation="RDTKRGLVRDELLPLLERLDPRARASLLSLADERPRLPRTLERT
                     LVELLSSRAGTKAADLGGGVRAVREYGTLRLEGAVEWGPWRLESEAPGLEVRARRAGD
                     RLAGRRKKVQDLLVDAKVPRAERDGWPLVVHGDEVVAVPGVAEAPGWEGVVRARRQ"
     gene            462..1037
                     /gene="hpt"
                     /locus_tag="VM638_03260"
     CDS             462..1037
                     /gene="hpt"
                     /locus_tag="VM638_03260"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HVM11481.1"
                     /translation="MPGGSDVEAVSVEEGVGEILIEAERLQARIRELGEEISNDYAGG
                     DLLLIGVLKGAVFFMADLMRELSVPCEIDFMAISSYGAQTDSSGVVRILKDLDTNIAG
                     RDVLVVEDIIDSGLTLSYLMRSLGARKPRSLEICALLTKPERREIEVPVRYVGFEIPN
                     RFVIGYGLDYAERFRNLPYVGVLADHLIPPH"
     gene            1104..>2488
                     /gene="ftsH"
                     /locus_tag="VM638_03265"
     CDS             1104..>2488
                     /gene="ftsH"
                     /locus_tag="VM638_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVM11482.1"
                     /translation="MNRFFRSALFPLIVIVLLVYLASQTLIPNREAEAQVTYSELIQQ
                     AESGNVQEVNFTPGRQRIDATLVGGDEVQVHYVSAESALAFEEILKRENVPYDSEGTG
                     GAWWSSLLITMLPFALLIAFWIFLMNQVQGGGSKVMSFGKSRAKRMTPDSPKIGFKDV
                     AGADEAVEELHEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARAVAGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFEQAKQSSPCIIFMDEIDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFEMKDNIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRNGRK
                     KILEVHSKGKPLAPEIELDTLAAGTPGFTGADLANLINEAALLAARRGKKVIGQDELE
                     EGIMRVIAGPEKKARLLGEKERKITAYHEMGHALVGHYLENTNPVHKITIVSRGQALG
                     LTISLPTEDRYL"
BASE COUNT          388 a          864 c          866 g          370 t
ORIGIN      
        1 ccgcgacacg aagcgtgggc tcgtccgcga cgagctgctg ccgctgctcg agcggctcga
       61 cccgcgtgcc cgcgcgtcgc tcctctctct cgccgacgag cgtccccggc tgccgcggac
      121 cctcgagcgg acgctcgtgg agctgctgtc gtcccgtgcc ggcacgaagg cggccgacct
      181 cggcggcggc gtccgcgcgg tgcgggagta cggcacgctg cggctcgagg gcgccgtcga
      241 gtgggggccg tggcggctcg agagcgaggc gccggggctc gaggtgcgcg cacgccgcgc
      301 cggcgaccgg ctcgcgggac ggcgcaagaa ggtgcaggat ctgctcgtgg acgcgaaggt
      361 gcctcgggcg gagcgggacg ggtggccgct cgtcgtgcac ggcgacgagg tcgtcgcggt
      421 gccgggtgtc gccgaggccc ccgggtggga aggagtcgtg cgtgcccgga ggcagtgacg
      481 tggaggccgt gtcggtggag gagggggtcg gtgagatcct gatcgaggcc gagcgcctgc
      541 aggcgcgcat ccgggagctc ggcgaggaga tctcgaacga ctacgccggc ggcgacctgc
      601 tcctgatcgg cgtgctgaag ggggccgtct tcttcatggc cgacctgatg cgcgagctct
      661 ccgtgccgtg cgagatcgac ttcatggcga tctcctcgta cggcgcgcag accgacagct
      721 caggcgtcgt ccgcatcctc aaggatctcg acacgaacat cgccggccgc gacgtgctcg
      781 tcgtcgagga catcatcgac tccgggctca cgctctcgta cctgatgcgg agcctcgggg
      841 cgcgcaagcc gcgctcgctc gagatctgcg ccctcctgac gaagccggag cggcgcgaga
      901 tcgaggtgcc ggtgcgctac gtcggcttcg agatcccgaa caggttcgtg atcggctacg
      961 gcctcgacta cgccgagcgc ttccgcaacc tcccctacgt cggcgtcctc gccgaccacc
     1021 tgattccgcc gcattagggg aagccccctt ctgcgggggg tcgtgcggca cggaggctgg
     1081 taccctggcc cgcaggagac cttttgaatc gcttcttccg cagcgctctc ttccccctca
     1141 tcgtgatcgt gctgctcgtc tacctggcga gccagacgct gatcccgaac cgggaggccg
     1201 aggcccaggt cacgtactcc gagctgatcc agcaggcgga gagcggcaac gtgcaggagg
     1261 tcaacttcac gcccggccgc cagcgcatcg acgcgacgct cgtcggcggc gacgaggtgc
     1321 aggtccacta cgtcagcgcc gagtcggctc tcgccttcga ggagatcctg aagcgggaga
     1381 acgtcccgta cgactcggag ggcaccggtg gcgcctggtg gagctcgctc ctgatcacga
     1441 tgctcccgtt cgcactcctg atcgcgtttt ggatcttcct catgaaccag gtacagggcg
     1501 gcggctcgaa ggtgatgagc ttcggcaagt cgcgcgcgaa gcggatgacg cccgactcgc
     1561 cgaagatcgg cttcaaggat gtcgccgggg ccgacgaggc ggtcgaggag ctgcacgaga
     1621 tcaaggagtt cctcgagaac ccgaagaagt tccaggcgct cggcgcgcgg atcccgaagg
     1681 gcgtgctcct ctacgggccg cccggaaccg gcaagacgct gcttgcccgc gccgtcgccg
     1741 gcgaggccgg cgtgccgttc ttctccatct ccggctccga cttcgtcgag atgttcgtcg
     1801 gcgtcggcgc ctcgcgcgtg cgcgacctct tcgagcaggc gaagcagtcg agcccctgca
     1861 tcatcttcat ggacgagatc gacgccgtcg gccgacaccg cggtgccggg ctcggcggcg
     1921 gccacgacga gcgcgagcag acgctgaacc agctgctcgt cgagatggac ggcttcgaga
     1981 tgaaggacaa catcatcctc atcgccgcca cgaaccggcc cgacatcctc gatcccgcgc
     2041 tgctccgccc gggccgcttc gaccggcaga tcgtcgtcga ccggcccgac cgcaacggcc
     2101 gcaagaagat cctcgaggtg cactcgaagg gcaagcccct cgcgcccgag atcgagctcg
     2161 acacgctcgc cgccggcacg cccggcttca ccggcgccga cctcgcgaac ctgatcaacg
     2221 aggccgcgct gctcgccgcc cgtcgcggca agaaggtgat cggccaggac gagctggagg
     2281 aggggatcat gcgcgtgatc gccggccccg agaagaaggc ccggctgctc ggcgagaagg
     2341 agcgcaagat caccgcctac cacgagatgg gccatgccct cgtcggccac tacctcgaga
     2401 acacgaaccc ggtgcacaag atcacgatcg tttcccgcgg gcaggccctc ggcctgacga
     2461 tctcgctccc gaccgaggac cgctacct
//
LOCUS       DATLGG010000116        10459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_24429, whole genome shotgun sequence.
ACCESSION   DATLGG010000116 DATLGG010000000
VERSION     DATLGG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24429"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VM638_03270"
     CDS             complement(<1..1490)
                     /locus_tag="VM638_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11483.1"
                     /translation="MRRMPSLVLAAVLVALVAAPVAAAEGPVTRPGASERVSVGLEGT
                     DPDASSSAPVISGNGRLVAFTSDATNLVTGDTNGTQDVFVHDRVTGRTERASVASSGA
                     EADGLSADPEISADGRFVAFTSAASNLVPEDTNGTYDVFVRDLVLGTTERVSVGADGA
                     QSPEGANDATINADGRYVGFSSSSTNLVPGDTNGWGDAFVRDRLAETTFRVSLGTGGV
                     EANLGGGDPDLSPDGGHVAFTSISTNLDGVDGSVGGDIFVRDLAADRTRLVSVGHDGS
                     PANEQSAVPAVSADGRFVVFRSRASNLVPGDRNTIHDFFVRDVEAGRTERVSVSSAGA
                     SGEGVWTGIPAPVITPDGRHVAFSTISPGLVPGDENQNLDVFVHDRVVGSTEIVTIRA
                     GGAQAHGTKASISDDGRHVGFLSDHGGIVPGDANDSADVFVRDRGPVLGPAVAEVTQA
                     GGFTEVQGWATFSGLSIASAVDPAGDASAGLAGSDLTGASIAYRPHT"
     gene            complement(1584..3341)
                     /locus_tag="VM638_03275"
     CDS             complement(1584..3341)
                     /locus_tag="VM638_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVM11484.1"
                     /translation="MRSRTALAALFLALSSLPLASPASAAAPPTGAVWDSVYFPSRDG
                     TMLHGDLLRPAGGCPEGGCPVIVSVGPYFGHGTQGSNAPLVPTPTYAGPSDRFYDFIA
                     HNFPGRGNIFQQGYAWLQVDSRGYGVSGGCNDYGGPGEQSDVAAAVEWAGTRPWSNGK
                     VGMWGKSYDAWTQVMGLAMNPPHLEAVVIQAPIIDGYGIAYVNGVHHDALWYATTSLY
                     QLYDWMPPAVGQAGRPEIIYPVAGLAGDCWPKQQALIAAGWDRDNLPANEYWRQRDLR
                     PSAGRNDDVAVLWSHGFNDINTKPDQIFGVYGRLNDLPKSNHRAWFGGWAHWRGNESQ
                     HTGRTGFLEESLDWFDHYLRGRPFRYSVNEKNVVEVQDNEGRWRTEATYPPADVKRIT
                     LPIRDGSYVDNNSTSATAGYWTFSQPLEHDLRIAGEPTVTLNADIAAPWGNVIVVLYD
                     HAANGQAREISRGAYRFSGDGPVTFTLHPRDHIVRKGRRLAIHVTSGHPQFLPHFTQQ
                     SIAISEASITVPFLHWERLVNLTGGQASSQARLLSGVPFSTGAAVEMPLPGPMEPYPT
                     DDEDETRERIQPSVVTPAG"
     gene            3461..4402
                     /locus_tag="VM638_03280"
     CDS             3461..4402
                     /locus_tag="VM638_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVM11485.1"
                     /translation="MSLMRRLMILRLLLRWRKQRRERPSASTPHRGAVRDPARAGRAP
                     QSPLDLEPRDWKRTLRGTLRQMKEDRISFAAAAVTYYLFLAIFPALIAVVGILGLAAV
                     DPSGLEEGVRAALPGGAGDVLADAVANANRPSQQTSLVAAVVGVAVALWSGSSGMVAL
                     QTGLNVAYDIPEERRFVKKRAVALALLLATLLLGGVPSPFFVLGEGALAATLGWVATA
                     LAVMLLFSIFYSLGPNRDAPRWRWVTPGGIVGGGLWIVLSLLFGLYISNFGDYGRTYG
                     ALAGVIVLLLWLYLSSLVVLVGAELNAELEDEADPAT"
     gene            complement(4533..6323)
                     /locus_tag="VM638_03285"
     CDS             complement(4533..6323)
                     /locus_tag="VM638_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11486.1"
                     /translation="MLKRSLLILAVGVPLVLPTAAHSVQLGGAPGGGASGSSHGNADL
                     ASPNMFHTANVPNPGVTNSDLAFWSEGGVRGPHQHLAAAGNYNGFRLFDIKDPDNPVL
                     VSDVRCRGPQNDVSFYKAGNRLLLIQSIDRPQTTEECATSADTPVAAGVPACEPTPEF
                     PEPDCPPGARILAQPGFEGLRIFDVSRPETPVHIASVPTACGSHTHTTIPDQDDQRAI
                     IYVSSYPTGSSPTPSTAQDFGGPRCTIPHARISIVEIEDDAPEEARVLKEQFLHADTM
                     PFLGTAGSGGTGAIGCHDITAYYEDQKQQPSGTQASQVKQNPRRVAGAACLEEGQIWD
                     ITQPENPTTLGPHTHIRNPFITSGIPGSFKGLFHTASFSWDGEVLLFTDEWAGGGGHG
                     CDGPQDTRGNVWFYKSVPPGTPDAPLYGRFITPRAQPAQEACTMHNGNVIPTNDGYFG
                     VSSAYEAGTSVFDFTGVQNRPTIFLGPPAPGTPGDPAPAPPIVAREIAFFDAQGVDGR
                     GRDDVWSSYWYNDRIYANGGLGRPHNPGGRGFDVYKLLTKNGRLVDANGNPTFGAGDN
                     ASDPRVKEYMARKQPYSNPQTQDTNQKVGG"
     gene            6499..7815
                     /locus_tag="VM638_03290"
     CDS             6499..7815
                     /locus_tag="VM638_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVM11487.1"
                     /translation="MRLRRLFGPATAFEGAEMDPRTLRFRDFDLERRFRAEFFRHTRP
                     NIRFGQVVGIVLWIAWGFVVRPYMLRSDQTIDLMVRYGLFIPVLLVGFGLSFTKAYER
                     IWEWAIAAVLVVTMIGWVYYVHELRTMPPDFGYVGLILITAFTYTLVRLRFVVVLGLT
                     LFASGVYLPYLFLSPGIFGIRVLLATFYLVSFGALGLMAAYRLEQFTRLLFVRERQLS
                     VERQRSDELLHNILPDAVIERLKTHEGSAPLAEEIPEVSVLFADAVAFTEQAGRTSPE
                     LLVGTLDELFRRFDELADRYGLEKIKTVGDAYMAVAGAPIPNPDHATAAAEMALAVVE
                     AAGELRWPSGDPVAMRVGLATGPAVAGVIGQRKFAYDLWGDTVNLASRLETYGQPGRI
                     LVAEEVASALDGRYELGPAEVVDLKGRGPTRVRFLLGRRGGSRTTG"
     gene            7840..8454
                     /locus_tag="VM638_03295"
     CDS             7840..8454
                     /locus_tag="VM638_03295"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HVM11488.1"
                     /translation="MAKRIFSIRPALSFRGRRFKGLRGWAGKPLHPPLTDFPIAAYAF
                     AALFDLISLFAGRGSGVARDFFVSATHVIIIGAVGGLAASLTGFWDWWKGIERSRGGL
                     VGRAKRTQVWRTTNWHMAVMLVVTALVALNIALRLGRFEQGYTDGLIAGLSLAAAALV
                     SFGAMYGGALVYDHQFNVESLEGSTAWDETERDQLPADKPPSEW"
     gene            8456..9157
                     /gene="folE"
                     /locus_tag="VM638_03300"
     CDS             8456..9157
                     /gene="folE"
                     /locus_tag="VM638_03300"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HVM11489.1"
                     /translation="MKEADRELDLRPDGDGSPAQGHGDPEEQRDWEQVRGRPIAPEDW
                     ERFERHVAEIFEAFGMDLDTPGTVNTPERFLRAIYDATSGYEGDPKLLTVFPTECRGG
                     PDCRLSQVIEGPISFFALCEHHALPFFGTAYVGYIAHESIIGISKLTRLVRLFARRFT
                     VQERMGQQVADSLVEMLRPHGVAVHLEAEHLCTQMRGVREEQSRTWTSFWRGAYEDEP
                     TLREEFLRVATRARS"
     gene            9166..10002
                     /locus_tag="VM638_03305"
     CDS             9166..10002
                     /locus_tag="VM638_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HVM11490.1"
                     /translation="MHPLEVLYERPGLEHGPIPERLARAYGGGIAFDRPAIVANFVSS
                     VDGVVALSTVPNAPSVIGGKSPHDRFVMGLLRASADAVVIGAGTLRAEPEHRWTPAHV
                     YPEAADDLAALRREGGLAPDPELVVLTAGGDLDPDIPALRGALVVTGATGAAEVARRG
                     VPAAAVVEAGERAVEPSRLVDILRARGHRLVLSEAGPLVTAQLLRDGLLDELFLTVSP
                     RVLGRVPDEHRPGLADGVDLLRAELPDAELLSVRRGADHLLLRYRFRRAARGGATPAD
                     AG"
     gene            10037..10300
                     /locus_tag="VM638_03310"
     CDS             10037..10300
                     /locus_tag="VM638_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVM11491.1"
                     /translation="MSEVPGGVVKVIELVGSSPNSFSDAVRNAVKTASETLRNIQGVE
                     VLSSNADVGENGELTLYKVNCKIAFLIERGGGQSDIVGEAGYS"
     gene            complement(10307..10444)
                     /locus_tag="VM638_03315"
     CDS             complement(10307..10444)
                     /locus_tag="VM638_03315"
                     /inference="COORDINATES: protein motif:HMM:NF023496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11492.1"
                     /translation="MRHAAWGEGAVQRYEAGKMVVLFDEGGYRTLATDLVEEQGLLEP
                     G"
BASE COUNT         1433 a         3596 c         3763 g         1667 t
ORIGIN      
        1 gtgtgcggcc ggtaggcgat cgaggcgccg gtgaggtccg acccggccaa gcccgcggag
       61 gcatctcccg cggggtccac cgccgacgcg atcgagaggc cggagaaagt cgcccagccc
      121 tgcacctcgg tgaacccacc cgcctgcgtc acctcggcaa ccgcgggacc gagcacgggg
      181 ccacggtcgc ggacgaagac atccgccgag tcgttcgcgt cgccgggaac gatcccgccg
      241 tgatcggaga ggaacccgac gtggcgcccg tcgtcgctga tcgacgcctt cgtcccgtgc
      301 gcttgcgcac caccggccct gatcgtcacg atctcggtcg aacccacgac ccggtcgtga
      361 acgaagacgt cgaggttctg gttctcgtca cccgggacga ggccgggcga gatcgtcgag
      421 aaggccacgt ggcggccgtc cggtgtgatg accggcgcgg ggatgccggt ccacacgccc
      481 tcgccggagg cgccggccga cgacacgctc acgcgctcgg tccgacccgc ttcgacatcg
      541 cggacgaaga agtcgtggat cgtgttgcgg tcgcccggga cgaggttcga ggcacggctg
      601 cggaacacca cgaacctccc gtccgcgctc acggcgggca cggccgactg ctcgttcgcc
      661 gggctgccgt cgtggccgac gctcacgagc ctcgtgcggt cggccgcgag gtcgcgcacg
      721 aagatgtccc cgccgaccga gccgtccaca ccgtcgaggt tcgtcgagat gctcgtgaac
      781 gcgacgtgtc cgccgtcggg actcaggtcg gggtcgccgc cgccgaggtt cgcctcgacc
      841 ccgccggtcc cgagcgacac tcggaacgtg gtctcggcga ggcgatcccg gacgaaggcg
      901 tcgccccagc cgttcgtgtc cccgggcacg aggttggtcg aggagctcga gaagccgacg
      961 tagcggccgt cggcgttgat cgtcgcgtcg ttcgcaccct cgggcgactg cgcgccgtcc
     1021 gcaccgacgc tcacccgctc cgtcgtcccg aggacgaggt cccggacgaa gacgtcgtac
     1081 gttccgttcg tatcctccgg gacgaggttc gatgcggcgc tcgtgaaggc gacgaagcgg
     1141 ccgtccgcgc tgatctcggg gtcggcggag agcccatccg cctcggcgcc gctcgaggcg
     1201 acggacgctc gctcggtccg gccggtcacc cggtcatgga cgaacacgtc ctgggtcccg
     1261 ttcgtgtcgc cggtgacgag gttcgtcgcg tcgctcgtga aggcgacgag gcggccgttc
     1321 ccgctgatga ccggcgccga ggacgaggcg tccgggtcgg tgccctcgag accgaccgag
     1381 acacgctcgc tcgcgccggg gcgggtgacg gggccttcgg ccgccgccac gggcgccgcg
     1441 acgagggcga ccaggaccgc ggcaagcacc agggacggca tccgcctcat gccgatgtct
     1501 tcgccggtcg gggagcgaac gcctctgtgc aggtgcccaa agcgcaccgg acggacggcg
     1561 gacgctccgc ccggcgacgg cggtcagccc gcgggggtca cgacgctcgg ctgtatccgc
     1621 tcgcgcgtct cgtcctcgtc gtcggtcggg tatggctcca tcgggccggg aaggggcatc
     1681 tccacagcgg ctcccgtgct gaacggtacc cccgacagga ggcgcgcctg cgaagaggcc
     1741 tgtcccccgg tgaggttcac gagccgctcc cagtgcagga acgggaccgt gatggaggcc
     1801 tccgagatcg cgatcgattg ctgcgtgaaa tgtggcagga actgcgggtg gccgctcgtg
     1861 acgtggatcg cgaggcgccg tcccttccgg acgatgtgat cccgcgggtg cagcgtgaag
     1921 gtcacgggcc catcgccgct gaagcggtag gcgccgcggc tgatctcccg cgcctgaccg
     1981 ttcgcggcat ggtcgtagag gacgacgatg acgttgcccc acggcgcggc gatgtcggcg
     2041 ttcagggtga ccgtcggctc gccggcgatc cgtaggtcgt gctcgagcgg ctgcgagaag
     2101 gtccagtagc cggcggtcgc ggaggtgctg ttgttgtcga cgtagctccc atcgcggatc
     2161 ggaagcgtga tgcgcttcac gtcggcgggc ggatacgtcg cctccgtgcg ccaccgcccc
     2221 tcgttgtcct ggacctcgac gacgttcttc tcgttcaccg agtagcggaa cgggcggccg
     2281 cgcaggtagt ggtcgaacca gtcgagcgac tcctcgagga agccggtccg gccggtgtgc
     2341 tgggactcgt tcccgcgcca gtgcgcccac ccgccgaacc aggcgcggtg gttcgacttc
     2401 ggcagatcgt tcagccgtcc gtagacgccg aagatctgat caggcttcgt gttgatgtcg
     2461 ttgaacccgt gcgaccacag gaccgcgacg tcgtcgttgc gcccggcgct cgggcggagg
     2521 tcgcgctgcc gccagtactc gttcgcgggc aggttgtccc ggtcccagcc cgccgcgatg
     2581 agcgcctgct gcttcggcca gcagtcgccc gccagacccg cgaccggata gatgatctcc
     2641 gggcgtccgg cctgaccgac ggcgggcggc atccagtcgt agagctggta gaggctcgtc
     2701 gttgcgtacc agagggcgtc gtggtggacc ccgttcacgt aggcgatccc gtagccatcg
     2761 atgatcggcg cctggatcac gaccgcctcg agatgcggcg ggttcatcgc gagccccatc
     2821 acctgggtcc acgcgtcgta gctcttcccc cacatgccga ccttgccgtt cgaccagggg
     2881 cgggtgccgg cccactcgac ggccgccgcc acgtccgact gctccccggg gccgccgtag
     2941 tcgttgcacc cgcccgagac gccgtatccg cgggaatcga cctggagcca cgcgtacccc
     3001 tgctggaaga tgttgccgcg cccggggaag ttgtgcgcga tgaagtcgta gaaccggtcc
     3061 gaggggccgg cgtacgtcgg tgtgggaacg aggggggcgt tgctgccctg ggtcccgtgc
     3121 ccgaagtacg gcccgacgga gacgatcacc gggcagccgc cctcggggca ccccccggcg
     3181 ggacgaagga ggtcgccgtg cagcatcgtt ccgtcacgcg acgggaagta gacggagtcc
     3241 cagaccgccc cggtgggcgg ggccgccgcc gaggcaggcg aggcgagcgg cagggaggac
     3301 agggcgagga acagggcggc gagcgcggtt cggcttctca tgccgggggg cttcgacggc
     3361 cgggggcggc agccctttgt gcaaccgccc aaagatgtcg gcaggtctgc ctcccctggc
     3421 tgccggtgtg cgcggcccct cccgggggta gagcgaggcc atgtccctga tgcgacggct
     3481 gatgatcctg cgcctcctcc tccgctggcg gaagcagcgc cgggagcgac cctcagcctc
     3541 gaccccccac cgtggggccg tccgggatcc cgcgagggcg gggcgggcgc cgcagagccc
     3601 gctcgacctc gagccgcgcg actggaagcg cacgctccgc ggcaccctcc gccagatgaa
     3661 ggaagaccgg atctcgttcg cggcggccgc cgtgacctac tacctgttcc tcgcgatctt
     3721 ccccgcgctg atcgcggtcg tcggcatcct cgggctcgcc gccgtcgatc cgtcgggact
     3781 ggaggagggc gtgcgcgccg ccctgcccgg aggcgccggc gacgtcctcg cggatgccgt
     3841 ggcgaacgcg aaccggccgt cgcagcagac gtcgctcgtc gcggccgtcg tgggtgtggc
     3901 agtcgccctc tggagcgggt cctcggggat ggtggcgctg cagaccgggc tgaacgtggc
     3961 ctacgacatc cccgaggagc gtcgcttcgt gaagaagcgt gccgttgccc tcgcgctgct
     4021 cctggcgacc ctcctgctcg ggggcgtccc ctcccccttc ttcgtcctcg gtgagggtgc
     4081 cctggcagca accctcggat gggtcgccac ggcgcttgcc gtcatgctcc tgttctcgat
     4141 cttctactcg ctcgggccga accgcgacgc gccgaggtgg cgctgggtca cgcctggcgg
     4201 catcgtcgga ggcgggctct ggatcgttct gtccctgctc ttcgggctgt acatctcgaa
     4261 cttcggtgac tacggccgca cctacggcgc gctcgccggc gtcatcgtgc tcctgctgtg
     4321 gctctacctc agctccctcg tcgtgctcgt cggagccgag ctgaacgccg agctggaaga
     4381 cgaggcagac cccgcgacct gatcagccgc agccgctgat ccgcccgtcc gccaccgcgg
     4441 gcccgccgcg ccgatatccg cccgggcgcg accgtaaacg cggacggggc gcccgaaggc
     4501 gccccgtccg tcgcagtcgc gcgggtcgcg cgctacccgc cgaccttctg gttcgtgtcc
     4561 tgcgtctggg ggttggagta cggctgcttg cgcgccatgt actccttcac cctcggatca
     4621 ctcgcgttgt cacccgcacc gaacgtcggg ttcccgttcg cgtccacgag ccgcccgttc
     4681 ttcgtcagca gcttgtacac gtcgaacccg cggccgcccg ggttatgcgg ccggccgagg
     4741 ccaccgttgg cgtagatccg gtcgttgtac cagtacgacg accagacgtc gtcccggccg
     4801 cgtccgtcca cgccctgcgc gtcgaagaac gcgatctccc tcgcgacgat cggcggtgcc
     4861 ggggcgggat cacccggggt gccgggcgcc ggcgggccga ggaagatggt cggcctgttc
     4921 tgcacgccgg tgaagtcgaa caccgaggtg ccggcctcgt acgccgagga gaccccgaag
     4981 tacccatcgt tcgtcgggat cacgttcccg ttgtgcatcg tgcaggcttc ctgcgcgggc
     5041 tgtgcgcgcg gcgtgatgaa ccgcccgtac agcggtgcgt cgggcgttcc cggcggaacg
     5101 ctcttgtaga accagacgtt cccgcgggtg tcctgcggac cgtcacagcc gtggcctccg
     5161 cctccggccc actcgtctgt gaacaggagg acctcaccgt cccagctgaa cgaggcggtg
     5221 tggaagaggc ccttgaagga ccccgggatc cccgaggtga tgaacgggtt gcggatgtgc
     5281 gtgtggggtc cgagggtcgt cgggttctcc ggctgggtga tgtcccagat ctgcccctct
     5341 tcgaggcagg cggcccccgc cacacggcgc gggttctgct tcacctgcga ggcctgggtg
     5401 cccgacggct gctgcttctg gtcctcgtag tacgcggtga tgtcgtggca cccgatcgct
     5461 ccggtgccac cgctccccgc cgtgcccagg aacggcatcg tgtcggcgtg gaggaactgc
     5521 tccttcagga cgcgggcttc ctccggcgcg tcgtcctcga tctcgacgat cgagatgcgg
     5581 gcgtgcggga tcgtgcagcg cgggcccccg aagtcctgtg ccgtggaggg cgtcgggctc
     5641 gagcccgtcg ggtacgacga cacgtagatg atcgcccgct ggtcatcctg atccgggatc
     5701 gtcgtatgtg tatgtgagcc gcaggccgtt ggcaccgatg cgatgtgcac cggagtctcc
     5761 ggacgcgaga cgtcgaagat ccgcaggccc tcgaagcctg gctgcgcgag gatgcgagcg
     5821 cccggcgggc agtccggctc cgggaactcc ggcgtgggct cgcaggcggg gacgcccgcc
     5881 gcgaccggcg tgtccgcgct cgtcgcgcat tcctcggtcg tctgcgggcg gtcgatcgac
     5941 tggatcaaga gcagccggtt gccggccttg tagaacgaga catcgttctg cgggccacgg
     6001 cagcggacgt cgctgacgag aaccgggttg tcggggtcct tgatgtcgaa gagacggaac
     6061 ccgttgtagt tcccggccgc cgcgaggtgc tggtgtggcc cacgtacccc tccttcgctc
     6121 cagaaggcga ggtcggagtt ggtgacaccg gggttcggca cgttcgccgt gtggaacatg
     6181 ttcgggctcg ccaggtcggc gttgccgtgc gacgagcccg acgcgccgcc tccaggggca
     6241 ccgcctagct gcacgctatg cgcagcggtg ggcagcacca gcggtacgcc caccgcgagg
     6301 atcaagaggg accgcttaag catgttccac cctcctcccg tcggacgggc gtccgactca
     6361 agggggagga cgctacgctc gtcacctttg cctgtcaaat ccccggttgt ccgctttgtc
     6421 cccgtcctaa ggtgcgtggg catgcaccgg acgcgccacg ctgtaggcgg catcatgggc
     6481 gccgtgagga tctcgctgat gcgcctccgt cgactcttcg gtccggcgac cgccttcgag
     6541 ggagcggaga tggatccgcg gacgctccgg ttccgcgatt tcgacctgga gcggcggttc
     6601 cgggcggagt tcttccgcca cacccggccg aacatccggt tcggccaggt cgttggcatc
     6661 gtgctctgga tcgcgtgggg cttcgtcgtc cgtccgtaca tgctccggtc ggatcaaacg
     6721 atcgacctga tggtccgcta cggcctcttc atcccggtgc tcctcgtcgg gttcggcctg
     6781 tcgttcacga aggcctacga gcgcatctgg gagtgggcga tcgcagcggt cctggtcgtg
     6841 acgatgatcg ggtgggtgta ctacgtccac gagctgcgga cgatgccgcc ggatttcggc
     6901 tacgtggggc tcatcctgat caccgcgttc acgtacacgc tcgtacggct ccggttcgtc
     6961 gtggtcctcg ggctgacgct gttcgcctcg ggcgtctacc tcccgtacct gttcctcagc
     7021 ccggggatct tcggtatccg ggtcctgctc gcgaccttct acctcgtctc gttcggagcg
     7081 ctcgggctca tggcggcgta ccgcctcgag cagttcactc gcctgctgtt cgtgcgggag
     7141 cgacagctga gcgtggagcg gcaacggtcc gacgagctcc tgcacaacat cctgccggac
     7201 gccgtgatcg agcgattgaa gacccacgaa ggcagcgccc cgctggccga agagatcccc
     7261 gaggtgagcg tcctgttcgc cgacgcggtg gcgttcacgg agcaggcagg gcggacatcg
     7321 ccggagctgc tcgtggggac gctggatgag ctgttccggc ggttcgacga gctcgccgat
     7381 cggtacgggc tcgagaagat caagacggtc ggggacgcgt acatggcggt cgcaggagcg
     7441 ccgatcccga accctgacca cgcaacggcg gccgccgaga tggcattggc cgtggtggag
     7501 gcggccgggg agctgcggtg gccctcgggg gaccccgtcg cgatgcgggt gggactggcg
     7561 accggcccgg ccgtcgcggg cgtgatcggc cagcggaagt tcgcctacga cctctggggt
     7621 gacacggtga acctcgcgag ccggctcgag acgtacggcc aaccgggtcg gatcctcgtc
     7681 gccgaagagg tcgcgtctgc cctcgacggc cggtacgagc tcggccccgc cgaggtcgtc
     7741 gacctgaagg gccgaggacc gacgagggtg cggttcctgc tcgggcggag gggcggatcg
     7801 cggaccacgg gatagactcg aggcggaagg aggcagatcg tggccaagag gatcttctcg
     7861 atccggccgg ccctttcgtt ccggggacga cggttcaagg gcctgcgggg ctgggccggg
     7921 aagcccctgc acccgccgct cacggacttc cccatcgcgg cctatgcctt cgccgcgctg
     7981 ttcgacctca tctcgctctt cgcgggccgg ggctccgggg tggcgcggga cttcttcgtc
     8041 tcggcgaccc acgtgatcat catcggcgcc gtgggagggt tggccgcctc gctgaccggg
     8101 ttctgggact ggtggaaggg catcgagcgg agccgcggcg ggctcgtcgg ccgggcgaag
     8161 cggacgcagg tctggcgcac gacgaactgg cacatggcgg tcatgctcgt cgtcacggcg
     8221 ctcgtcgcgc tgaacatcgc gcttcgcctc gggcgcttcg agcaggggta caccgacggc
     8281 ctgatcgccg gtctctcgct cgcggccgcc gcgctcgtgt cgttcggagc gatgtacggc
     8341 ggcgcgctgg tctacgacca ccaattcaac gtcgagagcc tcgagggatc gaccgcctgg
     8401 gacgagacgg aacgggacca gctgcccgcc gacaagcccc cgtcggagtg gtgagatgaa
     8461 ggaagcggat cgagagctcg acctccgccc cgacggggac gggtcccccg cgcagggtca
     8521 cggcgacccc gaagagcagc gggactggga gcaggtccgg ggccggccga tcgcgcccga
     8581 ggactgggaa cggttcgagc ggcacgtcgc cgagatcttc gaggccttcg gcatggacct
     8641 cgacacgccc ggaacggtga acacgccgga gcgcttcctc cgcgcgatct acgatgcgac
     8701 gtcggggtac gagggcgacc cgaagctgct cacggtgttc ccgacggagt gccgcggcgg
     8761 gccggactgc cggctgagcc aggtgatcga aggcccgatc tcgttcttcg ccctctgcga
     8821 gcaccacgcc ctccccttct tcggcacggc gtacgtggga tacatcgccc acgaatcgat
     8881 catcggcatc tcgaagctca cccggctcgt caggctgttc gcgcggcgct tcacggtgca
     8941 ggagcggatg ggccagcagg tggcggacag cctcgtcgag atgctgcgcc cgcacggggt
     9001 cgcggtgcac ctcgaggcgg agcacctctg cacgcagatg cggggcgtac gcgaggagca
     9061 gtcgcgcacc tggacctcgt tctggcgcgg tgcctacgag gacgagccga ccctgcggga
     9121 ggaattcctg cgggtcgcga cacgcgcgcg ctcctgacga cgaggatgca cccgctggag
     9181 gtcctctacg agcgcccggg gctggagcat gggccgatcc ccgaacggct cgcccgagcg
     9241 tacggcgggg ggatcgcgtt cgaccggccg gcgatcgtgg cgaacttcgt ctcctccgtc
     9301 gacggcgtcg tcgccctgtc gacggtcccg aacgcgccct cggtcatcgg cgggaagagc
     9361 ccgcacgacc ggttcgtcat ggggctcctc cgcgcctctg ccgatgcggt cgtgatcggc
     9421 gccgggacgc tccgcgcgga acccgagcac cgctggaccc ccgcccacgt gtaccccgag
     9481 gcggcggacg atcttgccgc cctgcgcagg gaggggggcc tcgcacccga cccggagctg
     9541 gtcgtcctga cggccggcgg cgatctcgat cccgacatcc cggcgctgcg gggcgccctc
     9601 gtcgtcacgg gcgcgacggg agcagccgag gtcgcgcgcc ggggcgtccc cgcggccgcg
     9661 gtggtggagg cgggcgaacg agcggtggag ccgtcgaggc tcgtcgacat cctgcgggcc
     9721 cgcggccatc gcttggtcct cagcgaggcg gggcccctcg tcacggcgca gctcctgcgg
     9781 gacggtctcc tcgacgagct gttcctgacc gtctctccga gggtcctcgg gcgtgtcccg
     9841 gacgagcatc ggccggggct ggcggacggg gtggacctcc ttcgcgcgga gcttccggat
     9901 gccgagctgc tgagcgtgcg ccggggcgcg gaccacctcc tcctccgcta ccgcttccgt
     9961 cgcgccgcgc gcggaggtgc gacaccggcg gatgccgggt agagtgcggc gcgtcgtcga
    10021 cccgaggagg tacccgatgt cggaggttcc cggcggtgtg gtgaaggtca tcgagctcgt
    10081 cggaagctcg ccgaactcgt tcagcgatgc ggtgcggaac gcggtgaaga cggcgtccga
    10141 gacgcttcgg aacatccagg gtgtcgaggt gctctccagc aacgccgacg tgggcgagaa
    10201 cggcgagctc acgctctaca aggtcaactg caagatcgcg ttcctcatcg agcgcggcgg
    10261 cggccagagc gacatcgtcg gcgaggccgg atactcgtaa gccgggctac cccggttcga
    10321 ggagtccctg ctcctcgacg aggtcggtgg cgagtgtgcg gtagcccccc tcgtcgaaga
    10381 ggacgaccat cttgcctgcc tcgtagcgct ggacggcgcc ctcaccccat gcggcgtggc
    10441 gcacgcgtga tccgatcgc
//
LOCUS       DATLGG010000117         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_179006, whole genome shotgun sequence.
ACCESSION   DATLGG010000117 DATLGG010000000
VERSION     DATLGG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179006"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VM638_03320"
     CDS             complement(<1..96)
                     /locus_tag="VM638_03320"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830094.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HVM11493.1"
                     /translation="MPYARIAAVGSALPERLVPNAEFEELVDTSDD"
     gene            complement(96..350)
                     /locus_tag="VM638_03325"
     CDS             complement(96..350)
                     /locus_tag="VM638_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVM11494.1"
                     /translation="MTKGDTTFRVLVSPAAGRLRFLPPKRFERGMEVVEAGQPIARVV
                     QGKGDLMVRAPFAGRVSAVMGLEGEPVQPGHALVAIEPHA"
     gene            complement(347..1081)
                     /locus_tag="VM638_03330"
     CDS             complement(347..1081)
                     /locus_tag="VM638_03330"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HVM11495.1"
                     /translation="MRSRGGPRPRGRGHRLLFAAGHSLGEFVALAFAGATEPMATLRV
                     VVERGAAMHDAGEENPGTMTALVGISPAEAEEIVRVAGRGDVLTVANENSPKQTVVSG
                     TIPAIERVEDLVRSRGAKAVRLKVAGAFHSSLMQPAAGRVRKAISALDFRQPRFPVVA
                     NASGRPTDQPSALRDLLGRHIVSPVKWERSMKAMADEGVDLFLEAGPGDVLTKLAKRC
                     VPGARAISVGSPQDVKTVIDEIGEGS"
     gene            1495..>2067
                     /gene="mreB"
                     /locus_tag="VM638_03335"
     CDS             1495..>2067
                     /gene="mreB"
                     /locus_tag="VM638_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HVM11496.1"
                     /translation="MSPSAVTHDRPEGLTGFLGRDMAVDLGTANTLVYVRGRGIVLNE
                     PSVVAINTINKQVLAVGAEAKRMIGRTPAHIQAVRPLKDGVIADFDITEKMLRYFIQK
                     VHTRRFLAKPRVVVCVPSGITGVEQRAVEEATISAGARAAYIIEEPMAAAIGANLPIH
                     EPTGNMVVDIGGGTTEVAIISLGGIVTATSV"
BASE COUNT          319 a          762 c          692 g          294 t
ORIGIN      
        1 gtcatcgctc gtgtcgacga gctcttcgaa ctccgcgttc gggacgagcc gctccgggag
       61 cgccgagccg acggcggcga tccgcgcgta gggcatcagg cgtgcggctc gatcgccacg
      121 agcgcgtgcc ccggctggac gggctcgccc tcgaggccca tgaccgccga gacacggccc
      181 gcgaacggcg cccggaccat caggtcgccc ttcccctgga cgacccgtgc gatcggctgg
      241 cccgcttcga cgacctccat gccgcgctcg aagcgcttcg gcggcaggaa ccgcagacgg
      301 ccggccgcgg ggctcaccaa caccctgaac gtcgtgtcac ccttcgtcat gaaccctctc
      361 cgatctcgtc gatgaccgtc ttcacgtcct gtgggctccc caccgagatc gcgcgcgctc
      421 cgggaacgca gcgcttcgcg agcttcgtga ggacgtcgcc gggccctgcc tccaggaaga
      481 ggtcgacccc ttcgtccgcc atcgccttca tcgagcgctc ccacttcacg ggcgagacga
      541 tgtggcgccc gagcaggtcc cggagcgccg acggctggtc ggtgggacgg ccgctcgcgt
      601 tcgccaccac cgggaagcgc ggctgccgga agtcgagcgc cgagatcgcc ttgcgcacgc
      661 gccctgccgc cggctgcatc aggcttgagt ggaacgcgcc ggcgaccttg agccggacgg
      721 ccttcgcgcc gcggctgcgg acgaggtcct cgacgcgctc gatcgcgggg atcgtgccgg
      781 agaccaccgt ctgcttcggg ctgttctcgt tcgcgaccgt caggacgtcg ccgcgacccg
      841 cgacccgcac gatctcctcc gcctcggcgg gggagatgcc gacgagcgcg gtcatcgttc
      901 cggggttctc ctcgccggcg tcgtgcatcg ccgcaccacg ctccaccacg acgcggagcg
      961 tcgccatcgg ctccgtggcc ccggcgaagg cgagggcgac gaactcgccg agcgaatgcc
     1021 cggcggcgaa gagaaggcga tgccctcggc ctcgaggacg cgggccgccg cgagatcgca
     1081 cgcgaaaagc gccggctgca cgatcgcggt gtccgagagc ttcgacgcgt cccggcacgc
     1141 ctcgacgacg tcgtagccga gcgtctcgga gcactcctcg aggaccgccc gccccgcggg
     1201 atgctccacc caggcgtcgg ccatccctgc gaattgggag ccctgaccgg ggaacagcac
     1261 gccgatcttc acgatgtctt cgacctcctg cgcatcggat ccacgtcgcc gcggttgcgg
     1321 tcagacggac ggacctcgga ccgaacgcgc aggttacaga ggggaaaagg ttcctatcta
     1381 gtaggtttcc gctcggtagg ttccggtgga gatcgcccct cgacccttgc cccggcgcca
     1441 aggttccggt agaaaaggct gacgccagcc ccgagatgcg tcatcctagg ccccatgagc
     1501 cccagcgccg tcacccacga ccgtcccgaa ggcctcaccg ggttcctcgg ccgcgacatg
     1561 gccgtcgacc tcggtacggc caacacgctc gtctacgtcc gcgggcgcgg gatcgtgctc
     1621 aacgagccgt cggtcgtcgc gatcaacacg atcaacaagc aggtcctggc cgtgggcgcg
     1681 gaggccaagc gcatgatcgg ccgcacgccg gcgcacatcc aggcggtccg cccgctcaag
     1741 gacggcgtga tcgccgactt cgacatcacc gagaagatgc tgcggtactt catccagaag
     1801 gtgcacacgc ggcgcttcct ggcgaagccg cgcgtcgtcg tctgcgttcc gagcgggatc
     1861 accggcgtcg agcagcgggc ggtcgaggaa gcgacgatct ccgccggcgc ccgcgccgcg
     1921 tacatcatcg aggagccgat ggcggccgcg atcggcgcga acctgccgat ccacgagccg
     1981 accggaaaca tggtcgtgga catcggcggc ggcacgaccg aggtcgcgat catctcgctg
     2041 ggcggcatcg tcaccgcgac gtcggtc
//
LOCUS       DATLGG010000118         8817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_55198, whole genome shotgun sequence.
ACCESSION   DATLGG010000118 DATLGG010000000
VERSION     DATLGG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55198"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VM638_03340"
     CDS             <1..1015
                     /locus_tag="VM638_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HVM11497.1"
                     /translation="MMALASVAAVPTQGRFAQIDVPTWGWVALLGSITALLIADLLIV
                     HRKPHAISLREAAVESSVWVALGLGFTGLVALWFGGQAAGEYITGYLIEKSLSVDNVF
                     VWAMLFTYFAVPREYQFRVLFWGIFGALVMRGLFIFAGVAVLARFEWVLFVFGGFLVF
                     TAVRMARHKGTEVHPEQNPVLRFVKRIVPSTHEYDGQKMFTRRSGRLLATPLFVILVL
                     VEATDVVFAVDSVPAILAISREPFIVYSSNAFAILGLRAMYFLLAGMAGRFRYLHLGL
                     AAILAFVGVKMLVAELYHPPVWLSLGFIATALTISITASLRADKRAVELALKEGPGET
                     PEG"
     gene            complement(1025..2296)
                     /locus_tag="VM638_03345"
     CDS             complement(1025..2296)
                     /locus_tag="VM638_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11498.1"
                     /translation="MMRRALRRRRGTEAFTSDALPDVASSQWWTSEQIAEYQARRICL
                     IVEHAYEETRFYRERMRRLGLRPSSIQGPDDLRAIPPVDRAEVQASRESILVVRDVHE
                     EALSGGTTGHRLRWARTIPWLELFTACLWRGFGWAGLDRGTKLVSFYSRMGMVSGNSV
                     IIEKAFELDTVEDDIRLIEESGAEVAYVYASAAYLLGRYLLETGRTLPLKSVITSCET
                     LYDEQRAVIERAFGAEVFDNYGCNDGGAWGAECTEHDGLHQDVERAVVEFVDGRMVST
                     DLWNTAFPFIRYANGDAGEWVPGACACGRSSPRMKLAGRVADLLVTPVRTIFPSTLNF
                     AFLKPWIRDARAIQRSALDVEVLVAPEGAATPTDLAGLEEELAGLFEGMRVSLRVVET
                     IPPTLSGKHRIGINLADVDPLPAPSPAPAGA"
     gene            2344..3714
                     /locus_tag="VM638_03350"
     CDS             2344..3714
                     /locus_tag="VM638_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HVM11499.1"
                     /translation="MEALETHVAFEERLRSAREELSAEAGTRRADGTTVGARLLEDVP
                     DALARLAARLPEGVVLVSGTNGKTTTTRMIAAVLEAAGLRPVSNRIGSNQAGGIAAEL
                     VDASGSDGGMRGTIGVFEVDELWLERVIDDLAPRVVVLTNLFRDQLDRLGEIDIVASR
                     WGRVASSASPGTRFVLCADDPRVAHLPVTDPDWFGIEDDGGAMTSLPNAADPMSCPDC
                     GSLHGYAAIHLGHLGRWSCATCGIERPAPHVAAVRVDTGAEGPRFELRTPEGSAQVGL
                     RIPGSFNVYNALAAASAARALRIPPSDTAAALSAITPAFGRGERVRVGAASLTLFLAK
                     NPTGLDEVLRALPLGAVHVLSLLNDSVYDGRDVSWIWDADLEQVADRAALIVCGGTRA
                     ADLALRWRYAGVPASRIRVAGSVEDALDRATERSSEISVIASYTAMLDVRDVLAERGW
                     VESVWR"
     gene            3711..4448
                     /locus_tag="VM638_03355"
     CDS             3711..4448
                     /locus_tag="VM638_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HVM11500.1"
                     /translation="MSAPHELRVAALYPERLNHYADRGNLIVLERRCRWRGIGFTLTR
                     VSVGDELHPSAHDLYYLGGGQDGDLARCGLDLAATKQVALHEAAARGAVVLGVCGGYQ
                     LLGRGYETGSAWVPGAGLVDVSTVAGSPRLVGHAVVEVDLGDGPRKLAGFENHAGRTW
                     LGTDEGPLGRVLTGHGNNGVDRTEGVRRGNVIGTYLHGPLLAANAWLADRLISIALGG
                     VPLEHLDDRFESAVHEAALAAAGSGRG"
     gene            complement(4434..5099)
                     /locus_tag="VM638_03360"
     CDS             complement(4434..5099)
                     /locus_tag="VM638_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HVM11501.1"
                     /translation="MAQVIRALPFAAASPRPVPLRDVRTLWEFLSGPDPLAPTDVLFC
                     FGSAHLGVARRAARLYRLGVSSRVLVTGGARGAIPPHRSEAETYAAVLLAEGVPADRI
                     VIEPLALNTGENVAMGMQALQRSGILVTNAGLVAWPPSLRRAQATFTHAFPGVPTLRY
                     PAFAGLGAWRGDHARAALWALDEMERLRAYPRLGFMAVTEIPSEVASAEERLRDLIAS
                     AAT"
     gene            complement(5141..6010)
                     /locus_tag="VM638_03365"
     CDS             complement(5141..6010)
                     /locus_tag="VM638_03365"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVM11502.1"
                     /translation="MGPASAGGDARPEGADDLLTLVADWPVKQAAVGVTDPDGTLGTG
                     GDEAWVAPIASVTKLLAGLAALVAVEEGAIELDEPAGPEGSTVRHLLAHASGLPFEGD
                     VPIAPPGKRRIYSNSGIERFAEHLARRAGMPFEEYLRLGVLEPLAMGATDLRGSPAHG
                     ARSSVADLLKLGRELLRPTLVAAETIALATTSQFANLGGVVPNIGRFEPNPWGLTFEI
                     RGEKEPHWTGRRNAPATFGHFGGSGTFLWVDPTAGLAAVALTDRTFGPWALQVWPAFS
                     DGMLERYAPSRGW"
     gene            6113..7129
                     /gene="aroF"
                     /locus_tag="VM638_03370"
     CDS             6113..7129
                     /gene="aroF"
                     /locus_tag="VM638_03370"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVM11503.1"
                     /translation="MVIVLRSSATEENLATLLERIRSFDLQPTVARAGDATLVTVIGE
                     DGIPAREFEGLAGVARVIPRVPAFKLASREVFAEDRVVDVAPGVRLGAGAVTLMAGPC
                     AVESRDQLEATAETLAAAGVRVLRGGAFKPRTSPYSFQGLGEKGLVLLREVADGYGMA
                     VVTEVLSPQEVGAVSEHADLLQIGARNMSNFALLRAAGEGPRPVLLKRGAGATIEELL
                     QAAEYVLAGGNENVILCERGIRTFERATRFTLDINAVPVLKSLSPLPVVVDPSHATGR
                     AEWVEPAARAAVAAGADGLLVEVHPTPEAALSDGDQSLDLPAFMSMVPALRRVAAAVG
                     RDLA"
     gene            7143..8279
                     /locus_tag="VM638_03375"
     CDS             7143..8279
                     /locus_tag="VM638_03375"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HVM11504.1"
                     /translation="MKQVPLPAPPLPQGRSLSEGLRAPELRFRVDELGTEVVIAPRSL
                     VQVGPRLRSALPHAAAAAVLADANVRDPADRVVGALEAAGFRVTRLDLPAGERAKTLE
                     AVKDVWHGLLDADIGRSDVVIAVGGGALLDAAGFAAATFRRGIALVNVPTTVLAMADA
                     ALGGKVAVDLAGAKNQVGAFHPATLVIVDPETLASLPSEEIRAGLAEVLKAAVLGSPE
                     ALAVLEEGDPWGGSALAWLIEEALRVKAGYVAADPHDHGARQALNLGHTFGHALESAS
                     GFSLRHGEAVAVGLVAAARLGQDLGLTPPDLTDRIVRSVEGLGLPLAPPPELDPARIL
                     EAIAADKKRRAGRAVFVVPTGAGAVLVEGLEPQRAVEALGVVVR"
     gene            8276..8719
                     /gene="aroQ"
                     /locus_tag="VM638_03380"
     CDS             8276..8719
                     /gene="aroQ"
                     /locus_tag="VM638_03380"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HVM11505.1"
                     /translation="MRILVLHGPNLNLLGEREPEVYGTLTLAEVDARIRAAAQERGVD
                     VRSEQSNLEGELVGHLQEARTWADGVILNPGGYSHTSVAIRDAVAAIPLPVVEVHLSN
                     LAAREEFRSRSIVAGACVGTIAGFGWPGYVLALDALRGLVSDRPR"
BASE COUNT         1181 a         3128 c         3172 g         1336 t
ORIGIN      
        1 catgatggct ctcgcgtcgg tggcggcggt tccaacgcag ggcaggttcg ctcagatcga
       61 tgtaccgacg tggggctggg tggcgctcct cgggtcgatc accgcgctgc tcatcgccga
      121 cctgctgatc gtccatcgca agcctcacgc gatcagcctc cgcgaggccg ccgtcgagag
      181 cagcgtctgg gtcgctctgg gtctgggttt cacggggctg gtggccttgt ggttcggagg
      241 gcaggccgcg ggcgagtaca tcacgggcta cctgatcgag aagagccttt cggtcgacaa
      301 cgtcttcgtc tgggccatgt tgttcactta cttcgccgtc ccgcgggagt accagttccg
      361 ggtcctgttc tgggggatct tcggcgcact cgtgatgcgc ggcctgttca tcttcgcggg
      421 cgtcgccgtc cttgcgcggt tcgaatgggt gctcttcgtg ttcggcggct tcctcgtctt
      481 caccgcggtg cgcatggcgc gtcacaaggg aaccgaggtt caccctgagc agaaccccgt
      541 gctgaggttc gtcaagcgca tcgtgccctc gacccacgag tatgacggtc agaagatgtt
      601 cacgcgtcgg agcggtcggc tcctcgccac accgctgttc gtcatcctcg tgctcgtgga
      661 ggccaccgac gtcgtgttcg ccgtcgactc cgtccccgcc atcctggcga tcagccgcga
      721 gccattcatc gtctactcgt cgaacgcctt cgcgatcctg ggtctgcgcg ccatgtactt
      781 cctgctcgcg gggatggccg ggcggttccg ctacctccac ctcggcctcg cggcgatcct
      841 cgcgttcgtc ggtgtgaaga tgctggtggc ggagctgtat cacccgccgg tgtggctctc
      901 gctgggcttc atcgccaccg cattgacgat ctccatcacg gcgtcgttgc gcgccgacaa
      961 gcgcgcggtc gagcttgcct tgaaggaagg cccgggcgag acacccgaag ggtaaggagg
     1021 cgtcctaggc gccggcgggc gcagggctcg gcgccgggag cggatcgacg tcggccaggt
     1081 tgatcccgat ccggtgcttc ccagaaagcg tcggagggat cgtctccacc acccgcagcg
     1141 aaacccgcat gccctcgaac aaccctgcga gctcctcctc cagaccggcg aggtcggtgg
     1201 gggtggcggc gccctccggc gcgaccagca cctcgacgtc caacgcggag cgctggatcg
     1261 cccgcgcgtc gcggatccag ggcttgagga aggcgaagtt gagggtcgac gggaagatcg
     1321 tcctcaccgg ggtgaccagc aggtcggcaa cacggccggc cagcttcatc cgaggggacg
     1381 accttccgca ggcgcacgcg ccggggaccc actcgccggc gtcgccgttc gcgtagcgga
     1441 tgaacgggaa ggcggtgttc cagagatccg tcgagaccat ccttccgtcg acgaactcca
     1501 ccacggcccg ctccacgtcc tgatgaagcc cgtcgtgctc ggtgcactcg gctccccagg
     1561 cgcccccgtc gttgcagccg tagttgtcga agacctcggc gccgaaggcc cgctcgatca
     1621 ccgctcgctg ctcgtcgtac agcgtctcgc agctggtgat cacgctcttg aggggcaggg
     1681 tgcgaccggt ctcgagcagg taccggccca gcaggtaggc ggctgaggcg tagacgtagg
     1741 cgacctcggc tccggactcc tcgatgaggc ggatgtcgtc ctcgaccgtg tccagctcga
     1801 aggccttctc gatgatgacg ctgttcccgg aaaccatccc catcctcgag tagaagctga
     1861 cgagcttggt ccctcggtcc aggcctgccc agccgaaccc tcgccagagg caggcggtga
     1921 agagctccag ccacgggatc gtgcgcgccc agcggagccg atggccggtc gtcccgccgc
     1981 tcagcgcctc ctcgtgcacg tcgcggacga cgaggatgct ctcgcggctc gcctgaacct
     2041 cggcgcgatc caccggaggg atcgcccgga gatcgtccgg tccctggatc gatgacggtc
     2101 gaagcccgag gcgccgcatc cgttcgcggt agaaccgcgt ctcttcgtag gcgtgctcga
     2161 cgatcaggca gatgcgacga gcttggtact ccgcgatctg ctccgacgtc caccactgcg
     2221 aggaagccac gtccggcagg gcgtcgctcg tgaacgcctc ggtccctctc ctccggcgca
     2281 gggcgcgtcg catcatggag ccgcgattct agccagtcgg gcgagccccg ctatcctgcc
     2341 cggatggaag cgctcgagac gcacgtggct ttcgaggagc gactgcgctc cgcccgcgag
     2401 gagctttccg cggaagccgg aacgcgtcgc gccgacggga cgaccgtggg ggcacggctc
     2461 ctcgaggacg tgcctgatgc gctcgccagg ctcgccgccc gcctgcccga aggggtcgtg
     2521 ctcgtctcgg gcacgaacgg gaagacgacc acgacgcgga tgatcgctgc cgtgctggag
     2581 gccgcggggc tccgtcccgt cagcaaccgg atcgggtcca accaggctgg cgggatcgcc
     2641 gcggagctcg tcgacgcttc cggatccgac ggaggtatgc gcggcacgat cggcgtcttc
     2701 gaggtggacg agctctggct cgagagggtc atcgatgacc tcgcgccgcg tgtcgtcgtc
     2761 ctgaccaacc tcttccgcga tcagctcgat cgcctcgggg agatcgacat cgtggcctcg
     2821 cgctggggca gggtggcgtc ctctgcgtcc ccgggaacgc gcttcgtgct gtgcgcggac
     2881 gatccccgcg tcgcgcatct gccggtcacc gaccccgact ggttcgggat cgaggacgat
     2941 ggtggcgcga tgacgtcgct cccgaacgcc gccgacccga tgtcctgccc cgactgcggg
     3001 tccctgcacg gctacgccgc gatccacctc ggccacctcg gacgctggtc gtgcgcgacc
     3061 tgcggcatcg agcgccccgc gccgcacgtc gcggcagtgc gtgtcgatac cggagcggaa
     3121 ggaccccggt tcgagcttcg cacgccggaa ggctccgctc aggtcgggct ccgcatcccg
     3181 ggatcgttca acgtctacaa cgcccttgcg gcggcctccg ccgcgagagc gctccggatc
     3241 ccgccctcgg ataccgcggc cgctctctcc gccatcacgc ccgccttcgg gcgcggtgag
     3301 agggtgaggg tgggcgccgc gagcctcacc ctcttcttgg cgaagaaccc gacgggcctc
     3361 gatgaggtgc tcagggcgct gcccctcggc gccgttcacg tcctctccct gctgaacgac
     3421 agcgtgtacg acgggcggga cgtctcgtgg atctgggacg ccgacctcga gcaggtcgcc
     3481 gaccgggccg ctctgatcgt ctgcggaggc acgcgggcgg ccgacctcgc cctccgctgg
     3541 cggtacgcag gcgtgcccgc atcgcgtatc cgcgtcgccg gatcggtcga ggatgcgctc
     3601 gaccgggcca ccgagaggtc ctccgagatc tcggtcatcg ccagctacac ggcgatgttg
     3661 gatgtccgcg acgtcctcgc ggagcgaggc tgggtggaga gcgtatggcg gtgagcgctc
     3721 cgcacgagct gcgcgtcgct gcgctctacc ccgagcgact gaaccactac gccgaccgcg
     3781 gcaacctgat cgtcctggaa cgccgttgcc ggtggcgcgg gatcgggttc accctgaccc
     3841 gcgtctcggt cggcgacgag ctccacccgt ccgcgcacga cctctactac ctcggtgggg
     3901 gccaggacgg cgacctggca cgatgtggcc tcgacctcgc cgcgacgaag caggtggcgc
     3961 tgcatgaggc tgccgctcgg ggcgccgtcg tgctcggcgt ctgcggcggc taccagctct
     4021 tgggtcgggg gtacgagacg ggatcggcgt gggttccggg ggcgggcctg gtggacgtga
     4081 gcaccgtcgc cggaagcccg cgactggtcg gccacgccgt ggtcgaggtc gacctcggcg
     4141 atggaccgcg gaagctcgcg ggcttcgaga accacgcggg ccggacctgg ctcgggacgg
     4201 atgagggccc gctcggccgc gtcctgacgg gccacggcaa caacggggtc gaccgcaccg
     4261 agggggtgcg tcgcgggaac gtgatcggga cgtacctgca cgggcccctg ctggccgcga
     4321 acgcgtggct cgccgaccgg ctcatctcga tcgccctcgg cggggtcccc ctggagcatc
     4381 tggacgaccg cttcgagtcc gccgtgcacg aagcggcgct cgccgccgcc ggatcaggtc
     4441 gcggctgagg cgatgagatc ccggagtcgt tcctccgcgc tcgcaacctc cgatgggatc
     4501 tcggtcaccg ccatgaagcc gagccggggg tacgcgcgga gccgctccat ctcgtccagg
     4561 gcccagagcg ccgcccgtgc gtggtcgcct cgccacgccc cgagcccggc gaaggcgggg
     4621 tagcggaggg tcggcacccc cgggaaggcg tgggtgaagg ttgcctgcgc ccggcggagg
     4681 gagggtggcc acgcgaccaa accggcgttg gtgaccagga tgccgctccg ctgcagggcc
     4741 tgcatcccca tcgccacgtt ctcacccgtg ttgagcgcca gaggctcgat cacgatcctg
     4801 tcggcgggca cgccctcggc gaggaggacc gccgcgtacg tctcggcttc gctccggtga
     4861 ggcgggatgg ccccgcgggc ccccccggtc accagcaccc ggctcgacac gccgagccga
     4921 tacaagcgag cggcgcggcg cgccacgccg aggtgggcgc tgccgaagca gaagaggacg
     4981 tcggtcggcg cgagcgggtc gggaccggac aggaactccc agagcgtccg aacgtcgcgc
     5041 aggggaaccg gccgcgggga ggcagcggcg aacgggagcg ctcggatcac ctgcgccacg
     5101 atgggtccct ggtccctgga gcgaacatcg acgcgcgatg ctaccaaccg cgcgacggtg
     5161 cgtaccgctc cagcatcccg tccgagaagg ccggccacac ctgaagcgcc caggggccga
     5221 aggtgcggtc ggtcagggcg accgccgcca gccccgccgt cgggtcgacc cagaggaacg
     5281 ttccgctgcc gccgaagtgg ccgaaggtcg ccggcgcgtt ccgccgcccg gtccagtgcg
     5341 gctccttctc gccacggatc tcgaacgtca ggccccacgg gttcggttcg aagcggccga
     5401 tgttcggcac gacgcctccg aggttcgcga actgggacgt cgtggcgagc gcgatcgtct
     5461 ccgccgcgac gagcgtcggg cggaggagct ccctgccgag cttgaggagg tccgcgaccg
     5521 agctccgggc gccgtgcgcg ggggatcctc gcagatccgt cgcacccatc gcgagcggct
     5581 cgagcacccc gagccggagg tactcctcga acggcatgcc ggcgcgccgc gccaggtgct
     5641 cggcgaagcg ctcgatcccg ctgttggagt agatccggcg cttccccggc ggcgcgatcg
     5701 gcacatcccc ttcgaacggg agccccgagg cgtgggcgag gaggtgccgg accgtcgatc
     5761 cctccgggcc cgccggctcg tcgagctcga tcgccccctc ctcgacggcg acgagcgcgg
     5821 cgagacccgc gagcagcttc gtcaccgacg cgatgggggc cacccacgct tcgtcgcccc
     5881 cggtgccgag ggtgccatcc ggatccgtca cccccaccgc ggcctgcttg accggccagt
     5941 ccgccacgag cgtcaagagg tcgtccgcgc cctccggacg cgcgtctccc cctgccgatg
     6001 ccggtcccat cggttctcca ccctagggcc gctgctgcgc gagcgcccac ccggggggcg
     6061 agggctcgaa ccgcttcggc ccgtcggcta ggcttccgtg cgggaggagc cgatggtcat
     6121 cgttctgaga tcgagcgcga ccgaggagaa cctcgcaacg ctgctcgagc ggatccgctc
     6181 gttcgacctc cagccgacgg tcgcgcgcgc cggcgacgcg acgctcgtca cggtgatcgg
     6241 tgaggacggc atccccgcgc gagagttcga ggggctggcg ggggtcgcgc gcgtgatccc
     6301 gcgggtcccc gcgttcaagc tcgccagccg ggaggtcttc gccgaggacc gcgtcgtgga
     6361 cgtcgcgccc ggcgtccggc tcggtgccgg tgccgtcacg ctgatggcgg ggccgtgcgc
     6421 ggtcgagagc cgggaccagc tcgaggcgac cgccgagacg ctcgcggcgg cgggcgtccg
     6481 cgtgctgcgc ggcggcgcgt tcaagccgcg gacctcgccg tacagcttcc agggcctcgg
     6541 tgagaagggg ctggtcctgc tgcgcgaggt ggccgacggc tacgggatgg ccgtcgtcac
     6601 cgaggtgctc agcccgcagg aggtgggggc ggtctccgaa catgcggacc tcctgcagat
     6661 cggcgcgcgc aacatgtcga acttcgccct cctccgcgcg gccggcgaag ggccacgacc
     6721 cgtcctcctc aagcgcgggg cgggggcgac gatcgaggag ctcctccaag ccgcggagta
     6781 cgtcctcgcg ggcgggaacg agaacgtgat cctgtgcgaa cgcggcatcc gcacgttcga
     6841 acgtgcgacc cgcttcaccc tcgacatcaa cgcggtcccg gtgctcaagt cactctctcc
     6901 gctgccggtc gtcgtggacc ccagtcacgc caccgggcgc gccgagtggg tggagcccgc
     6961 cgcgcgcgcg gccgtcgcgg ccggcgccga cgggctcctc gtcgaggtgc atccgacccc
     7021 cgaggctgcg ctctccgacg gcgaccagtc gctcgacctt cccgccttca tgtcgatggt
     7081 tccggccctc cgccgggtcg cggctgcggt ggggcgcgat ctcgcgtgag ccgggcgcgt
     7141 ccgtgaagca ggtcccgctc ccggctccgc ccctcccgca aggacggtcc ctgagcgagg
     7201 ggctccgagc gccggagctg cgcttccgcg tcgacgagct ggggaccgag gtcgtcatcg
     7261 ctccgcgttc gctcgtgcag gtgggaccgc gcctgcgttc cgcgcttccc catgcggccg
     7321 cggcggcggt cctggcggat gcgaacgtgc gtgatcccgc ggatcgggtc gtgggtgcgc
     7381 tcgaggcggc gggcttccgc gtgacccgcc tcgacctgcc ggcgggggag cgggcgaaga
     7441 ccctcgaggc ggtgaaggac gtctggcatg ggctgctgga cgcggacatc ggccggtcgg
     7501 acgtggtcat cgcggtgggg ggcggagccc tcctcgatgc cgccggattc gccgccgcga
     7561 cgttccgccg cgggatcgcc ctcgtgaacg tcccgacgac ggtgctcgcg atggccgacg
     7621 ccgcgctcgg cgggaaggtc gcggtcgacc tcgccggagc gaagaaccag gtgggggcgt
     7681 tccacccggc gaccctcgtg atcgtcgatc cggagacgct cgcgtccctg ccatcggagg
     7741 agatccgggc ggggctcgcg gaggtcctga aggcggccgt gctcggttcg cccgaggcgc
     7801 tggcggtgct cgaggagggg gacccgtggg gaggttccgc gttggcgtgg ctgatcgagg
     7861 aggcgcttcg ggtcaaggcc ggctacgtcg ccgccgaccc gcacgaccac ggcgcccgac
     7921 aggccctgaa cctcggtcac acgttcgggc acgcgctcga atcggcttcc gggttctcgc
     7981 tccgtcacgg cgaggcggtc gcggtgggcc tcgtcgcggc cgcgcggctc ggccaggatc
     8041 tcggtctcac tccgcccgac ctcaccgatc ggatcgtccg ctcggtcgaa ggcctcggcc
     8101 tgccgctcgc cccaccgccc gagctcgacc cggcgcggat cctcgaggcg atcgcggccg
     8161 acaagaagcg ccgggccggc cgtgcggtct tcgtcgtgcc gacgggtgcc ggcgccgtcc
     8221 tcgtcgaggg gctcgaaccg cagcgtgcgg tcgaggcgct gggggtcgtg gtgcgatgag
     8281 gatcctcgtc ctgcacgggc cgaacctgaa cctgctgggg gagcgtgagc cggaggtgta
     8341 cggaaccctc accctcgccg aggtcgatgc gcggatccgg gcggcggcgc aagagcgtgg
     8401 cgtcgacgtg cgcagcgagc agtcgaacct cgagggcgag ctcgtgggcc acctccagga
     8461 ggcccggacg tgggccgacg gcgtgatcct caaccccggc gggtactcgc acacgagcgt
     8521 ggcgatccgc gacgcggtcg cggcgatccc gcttccggtc gtggaggttc acctctcgaa
     8581 cctcgcggcg cgcgaggagt tccggagccg gtcgatcgtc gccggcgcct gcgtcgggac
     8641 gatcgcgggg ttcggatggc cgggctacgt cctcgcgctc gacgcgctcc gcggcctcgt
     8701 cagcgatcga ccccgctgac gcgggcgtct tcccggcgaa cgagccagac cttgcccgac
     8761 ggcagccgat cgagatcgtc gatgtcgacg acgcggatct cggtgtcggg gtccgtc
//
LOCUS       DATLGG010000119         2940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_134911, whole genome shotgun sequence.
ACCESSION   DATLGG010000119 DATLGG010000000
VERSION     DATLGG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134911"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VM638_03385"
     CDS             <1..1363
                     /locus_tag="VM638_03385"
                     /inference="COORDINATES: protein motif:HMM:NF036452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5305 family protein"
                     /protein_id="HVM11506.1"
                     /translation="RRLVLHRIVRIQGEHFVLKGDANTWLDSDKPTQAEIVGRTWIHL
                     PVGGGILAWLRTPINAAIVAGIFGLLAFGGAANRRSRRNRLRRGRKDAPPRRAPIPEP
                     QAGYGRLAPLGGAAGGAIGAIVILVFALSRPASVERTEEIAYTHTGDFTYSAEASPSQ
                     AYEADVKTGDPVFVRLAPVVDVGFEYRLDSQIPAALSGTIGLSAELQDGAGWRRRIPV
                     TAPVPFRGMSTTAEGTIDLRRVNELLRDVERDTGIGRPSVILRLFADVHLRGKVGGAP
                     IDERFVPNLPFELEPLMLRLYDPVGDAPPTEALRPTKQGTATVVRDEPNRIPLPGLRF
                     DVAGARAASAVAVPLFLLALALTSRSALRLRPRSESERIRQRHARRIVPLYRTDHLSA
                     QATVDVTSMEALVRLAETYERMVMQIDREDVDEYLVEGDVVVYRYRVAKPLDEDAAVG
                     EAG"
     gene            1409..1951
                     /locus_tag="VM638_03390"
     CDS             1409..1951
                     /locus_tag="VM638_03390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVM11507.1"
                     /translation="MTVRTVRTRDLPPDELAALRSFLEEAWLDEPEGFSQEDWEHALG
                     GLHFVIEEDGRPVAHAAVVERTIHADGHELRAGCVEAVATAPARRCRGHGSAVMREVG
                     RYIDGSFELGALLSTGQHAFYERLGWRVWRGPTFVRLGGELVRTPDDDGSVLVRLTPL
                     SPELDLTAPISCDWRPGDAW"
     gene            complement(1968..>2940)
                     /locus_tag="VM638_03395"
     CDS             complement(1968..>2940)
                     /locus_tag="VM638_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11508.1"
                     /translation="FDINRTIDTFVHDRLTGATERISVTTDGEERAGDSYGTPGLSGD
                     GSIVAFTSDATLVPGDGNAERDVFVRDRGAPLGVAGLRADRSGSTVEVTGRASFAGAL
                     AEASDPTGDGLPAGADLRSVSIAYRPQRGDLLVRWVPTEMPTLRGPGGLGTAFEVRGG
                     TVPTAAAGPAYELRVTSGGRSYVIRVDAAGASLLHCEAACARPVPLDGSFGTTGVAAT
                     AEVPLSFLGARPGETLSALHATAGAAAQATADDVSLGSMLLAEPNVDVGLTFAGTPPD
                     EVTFHRAELDLGAFRGSAALDASGPHDVWARACLGDTCHATRTTLDP"
BASE COUNT          413 a         1023 c         1074 g          430 t
ORIGIN      
        1 acgtcgcctg gtccttcacc ggatcgtgcg catccagggg gagcacttcg tcctgaaggg
       61 tgacgcgaac acctggctgg actccgacaa accgacgcag gcggagatcg tcgggcggac
      121 gtggatccac cttcccgtcg gcggtgggat cctcgcgtgg ctccgaacgc cgatcaacgc
      181 ggcgatcgtc gccggcatct tcggcctgct cgcgttcggt ggagcggcga accggcgctc
      241 acgtcgcaac cggctccgga gggggcggaa ggacgcgcca cctcgtcgtg cgcccatccc
      301 tgagccgcag gccggctacg ggcggctcgc accgctcggg ggggcggccg gcggtgcgat
      361 cggagcgatc gtcatcctcg tgttcgccct gtcccgcccg gcgagcgtcg agaggacgga
      421 ggagatcgcc tacacgcata ccggcgattt cacgtattcg gctgaggcgt cgccgagcca
      481 ggcgtacgaa gcggacgtca agacggggga tcccgtcttc gtgcggctcg ctccggtcgt
      541 cgacgtcggc ttcgagtacc gcctcgatag ccagatcccc gcggcgctgt caggaacgat
      601 cggtctcagc gccgagcttc aggacggagc cgggtggcgt cgacggatcc cggtaacggc
      661 accggtcccg ttccgcggca tgagcacgac cgccgagggg acgatcgatc tccgtcgtgt
      721 gaacgagctc ctccgcgacg tcgagcgtga caccgggatc gggcgcccga gcgtgatcct
      781 tcgcctcttc gcagacgttc acctccgcgg caaggttggg ggggcaccta tcgatgagcg
      841 gttcgtgccg aacctcccct tcgagctcga accgctgatg cttcggctgt acgacccggt
      901 cggcgacgcg ccgccgaccg aggcgctccg gccgacgaag cagggaaccg cgacggtcgt
      961 gcgcgatgag ccgaaccgca tccccctccc cgggctgcgc ttcgacgtcg cgggcgctcg
     1021 tgcagcgtcg gccgtcgcgg ttcccctttt cctcctcgcc ctggcgctga cgtcgcgttc
     1081 ggcgctccgc ctccggccgc gcagcgagtc cgagaggatc cgtcaacggc atgcccgccg
     1141 catcgtgccc ctgtaccgaa cggatcatct gagcgcgcag gccacggtcg acgtcacgtc
     1201 gatggaggcg ctcgtccgac tcgccgagac gtacgagcgg atggtcatgc agatcgatcg
     1261 agaagacgtc gacgaatacc tcgtcgaggg agacgtcgtc gtgtaccgct atcgggtcgc
     1321 gaaaccgctc gacgaagatg ctgcggtcgg ggaggccgga taaccggaga tcgtgccccg
     1381 gggagcgccg ccgccgtatc gtggttcggt gactgtccgc accgtccgga cgagggatct
     1441 cccgcccgac gagctcgccg cgctccgatc gttcctggag gaagcctggc tcgacgagcc
     1501 ggaggggttc tcccaggagg actgggaaca cgccctcggt gggctgcact tcgtcatcga
     1561 ggaggatggc cggcccgtcg cgcacgccgc cgtggtcgag cggacgatcc atgccgacgg
     1621 gcacgagctg agggcgggct gcgtcgaggc agtggcgacc gctcctgccc gacggtgccg
     1681 ggggcacgga tcggcagtga tgcgggaggt cgggcgctac atcgacggat cctttgagct
     1741 cggtgcgctg ctcagcaccg gtcaacacgc gttctacgag cggctggggt ggcgcgtgtg
     1801 gcggggcccg acgttcgtcc ggctcggggg agagctcgtc cgcacgcctg atgacgacgg
     1861 gtccgtcctc gtgcggctga ccccgctgtc accggagctc gacctgaccg ctccgatcag
     1921 ctgcgactgg cgtcccggcg acgcgtggta aggcccggtc ggcccgctca gggatctagt
     1981 gtggtgcgcg tggcgtggca ggtatcgccg aggcacgcgc gagcccagac gtcgtggggt
     2041 ccggaggcgt cgagcgcagc ggagcctctg aacgctccga ggtcgagctc ggcgcgatgg
     2101 aacgtgacct cgtccggcgg cgttcccgca aaggtgaggc ccacgtccac gttcggttcc
     2161 gctaggagca tcgagccgag gctcacgtcg tccgccgtgg cctgggcggc agcgcctgcc
     2221 gtcgcgtgca gggccgagag cgtctcgccc gggcgagcgc cgaggaagga gagcgggacc
     2281 tccgccgtcg ccgcgacgcc ggtcgttccg aaggagccgt cgagtggcac cgggcgcgca
     2341 cacgccgcct cgcagtggag gagcgacgcc ccggcggcat ccacccggat cacgtaggag
     2401 cggccgccgc tggtcacgcg gagctcgtag gccggacccg ccgccgccgt cggcaccgtc
     2461 ccgccccgga cctcgaacgc cgtcccgaga ccgcccggac cgcggagggt cggcatctcg
     2521 gtgggcaccc agcggaccag caggtcgccg cgttgcggcc ggtaggcgat cgagaccgag
     2581 cgcaggtccg cgccggcggg gagcccgtcg ccggtcgggt cgctcgcctc cgcgagggcg
     2641 ccggcgaagc tcgcccgtcc cgtcacctcg accgtcgagc cgctgcggtc cgcgcggagc
     2701 cccgcgacac cgaggggggc gccgcggtcg cggacgaaga catctcgctc ggcgttcccg
     2761 tctccgggga cgagtgtcgc gtcggaggtg aaggcgacga tgctgccatc gcccgacagc
     2821 cccggcgtgc cgtaggaatc acccgcgcgc tcttcgccgt cggtcgtgac ggagatccgc
     2881 tcggtcgcgc cggtgagccg gtcgtgcacg aacgtatcga tcgtccggtt gatgtcgaac
//
LOCUS       DATLGG010000120         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_87047, whole genome shotgun sequence.
ACCESSION   DATLGG010000120 DATLGG010000000
VERSION     DATLGG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87047"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VM638_03400"
     CDS             complement(<1..809)
                     /locus_tag="VM638_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11509.1"
                     /translation="MSEHPDAPKERPARGAPRGTVVELVRLILVALFAVAGWQIAGAI
                     RVDEGQILLGIVLGSAIGYVLGGIVGRRTVVAVSAAEREIRKIPPSELLAGSLGLVVG
                     LVVSVLLSFILFRFPPEVGFPVSAFLTVVLSYLGYRVGRGVHQELFGLFGLKPRAAGV
                     RPGEVNVLDTSALIDGRILDVVGAGFLTGTFLVSRGVIAELQSIADASDPRRRARGRR
                     GLDVLEVLQASPAIEIAVIDEPPSGDVDADLVRLARERGGALVTTDANLAKV"
     gene            complement(850..1344)
                     /locus_tag="VM638_03405"
     CDS             complement(850..1344)
                     /locus_tag="VM638_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HVM11510.1"
                     /translation="MSYRKGDKVIYPHHGAAVIEDLTEREFLGEKKKYLILRLAYGDL
                     TLMVPVESTEEVGLRDVVSKREVRKVMEVLRQAETRMPTNWSRRYKTNIEKIKSGDIY
                     QVAEVVRNLSLREKEKGLSAGEKRMLAKARQILISELVFAVDQPEEKAEAMVDRVLEE
                     AHTA"
     gene            complement(1429..2340)
                     /locus_tag="VM638_03410"
     CDS             complement(1429..2340)
                     /locus_tag="VM638_03410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HVM11511.1"
                     /translation="MLEAARAERPDLLVVDSIQALRDPEQTTLPGGPSQVRACTDALV
                     SLAKRDGIAVVLAGQVTKDGDLAGPRTLEHAVDVVCSFDGDPRTGLRVLAGGKNRFGP
                     EGEVAWMEMGPAGLTEVDPATVLGRSAEPGAAAAVVVMGRRGLAVEIQALVVPGDGPS
                     RRHVSGLDPRRFAVVAAVVDRVQGLRLAKAELYGASAGGLRIEEPGADLAVAAALASA
                     ATGTAAPPDSAFVGEVALTGEVRPVPNPAARVAAAGAAGIRTLYCGGDPGPVPKGIRT
                     VRVAHVRDAIAWSRGRPAGAPVAHSGP"
BASE COUNT          373 a          891 c          841 g          323 t
ORIGIN      
        1 accttggcga ggttggcatc ggtcgtgacg agcgcgccgc cgcgctcccg cgcgagccgg
       61 acgaggtcgg cgtcgacgtc gcccgatggg ggctcgtcga tcacggcgat ctcgatcgct
      121 ggcgaggcct ggagcacctc cagcacgtcg aggccgcgtc ggccacgggc ccgacgacga
      181 gggtcggacg cgtcggcgat ggactggagc tcggcgatca ccccgcgaga gacgaggaag
      241 gtgcccgtga ggaatcccgc gccgacgacg tcgaggatgc ggccgtcgat cagcgcgctc
      301 gtgtcgagca cgttcacctc cccgggtctc acgcccgcgg cgcgcggctt cagcccgaag
      361 agcccgaaga gctcctggtg gacgccacgg ccgacgcggt agccgaggta ggaaaggacg
      421 accgtgagga aggcggagac cgggaagccg acctcgggcg ggaaccggaa gaggatgaag
      481 gacaggagca ccgagacgac cagccccacg accaggccga gcgatcccgc cagcagctcc
      541 gacggcggga tcttccggat ctcccgctcc gccgccgaga cggcgacgac cgtccgacgc
      601 ccgacgatcc ccccgaggac gtacccgatc gcgctgccca gcacgatgcc gagcaggatc
      661 tgaccctcgt cgacgcggat cgcaccggcg atctgccatc cggccaccgc gaacagcgcg
      721 acgaggatga gacggacgag ctcaacgacc gtcccccgcg gggctccgcg ggctggtcgc
      781 tccttcggcg cgtcggggtg ctcgctcacg ttcctagcct gcgccaggaa cgaggcccgt
      841 acaagggcgc taggcggtgt gggcttcctc gaggacgcgg tcgaccatcg cctcggcctt
      901 ctcctcgggc tggtcgacgg cgaacacgag ctcggagatg aggatctggc gcgccttcgc
      961 gagcatgcgc ttctcgcccg ccgagaggcc cttctccttc tcccggaggg acaggttccg
     1021 cacgacctcg gcgacctgat agatgtcgcc ggacttgatc ttctcgatgt tggtcttgta
     1081 gcgccggctc cagttggtcg gcatgcgggt ctcggcctgc cgcaggacct ccatgacctt
     1141 ccggacctcg cgcttgctga ccacgtcccg cagcccgacc tcctcagtgc tctcgaccgg
     1201 gaccatcagg gtgaggtcgc cgtaggccag gcggaggatc aggtacttct tcttctcgcc
     1261 gaggaactcg cgctccgtga ggtcttcgat gacggcggct ccgtggtgcg gatagatcac
     1321 tttgtcacct ttgcggtacg acacacgacc tccctgcgct cggaaaacag ctcgacgtaa
     1381 ggccatgata gcagggtgga cagtcggctg acaaatcaaa accgctggtc acgggcctga
     1441 atgagccacc ggagcgccgg ccgggcggcc gcgcgaccag gcgatcgcgt cgcgcacgtg
     1501 cgccacacgc accgtccgga tcccctttgg aaccggcccg gggtctccgc cgcagtagag
     1561 cgtcctgatc cccgcggcgc cggcggccgc gacccgtgcg gccgggttcg gaaccgggcg
     1621 gacctccccc gtcagcgcca cctcgccgac gaaggctgag tccggcggcg cggcggtgcc
     1681 ggtggcggcg gaagcgagcg ccgcggcaac cgcgaggtcc gcccccggct cctcgatccg
     1741 gaggccgccc gcggaggcgc cgtacagctc cgccttcgcg aggcggagcc cctgcacgcg
     1801 gtcgacgacg gccgcgacga ccgcgaaccg acgggggtcg agacccgaga cgtggcgcct
     1861 cgaaggcccg tcccccggga cgaccagggc ctggatctcg accgcgagcc cccgccgtcc
     1921 catgacgacg acggcggccg cggcgccggg ttcagcggag cgccccagga ccgtcgccgg
     1981 atcgacctcg gtcagccccg cgggacccat ctccatccac gcgacctcgc cttccgggcc
     2041 gaagcggttc ttcccgcccg cgagcacgcg aagtcccgtc cgcgggtcgc cgtcgaacga
     2101 gcaaacgacg tcgacggcgt gctcgagcgt ccgtggcccg gccaggtcgc cgtccttcgt
     2161 cacctgaccc gcgaggacga cggcgatccc gtcccgcttc gccagcgaca cgagtgcgtc
     2221 ggtgcacgcc cggacctgcg acggcccgcc ggggagggtc gtctgctccg gatcgcgcag
     2281 cgcctggatc gagtccacca ccaggagatc gggtcgctcg gcgcgtgcgg cctcgagcac
     2341 ctcggtcaga tcgcgccccg ggacgaaccg atctcctggt ggtggactcg atccaggcgc
     2401 tgcgcgatcc ggagcagacg accctccc
//
LOCUS       DATLGG010000121         4318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_164954, whole genome shotgun sequence.
ACCESSION   DATLGG010000121 DATLGG010000000
VERSION     DATLGG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164954"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VM638_03415"
     CDS             complement(<1..425)
                     /locus_tag="VM638_03415"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVM11512.1"
                     /translation="MPRNGRRQAILDAAVGLFSDRGYHGTSVRDIAQETGMLSGSLYA
                     HIASKEDLLHEIVTRAAGQFLAGVEPVARAELPPEEKLRRALRAHVRVVAGSIDEARV
                     FLHEWRALSGARLEEVRALRERYERLWAGIVDEGVSAGA"
     gene            complement(464..1315)
                     /locus_tag="VM638_03420"
     CDS             complement(464..1315)
                     /locus_tag="VM638_03420"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HVM11513.1"
                     /translation="MDESQIGGIAFIGVGGFLVLLALFIVWRTKRFLRDAVTVPGTVT
                     GLAMGSGGEGGTVYHPIVSFTAMDGQEIQFQDKLGSNPPSHQPGEMVEVRYLPSNPQK
                     ARLAGRFRTWFVPGLLGLLGIVFVGVGIPWYLSAGGDPSETRADPTVPATIPTNLPGV
                     VLPTDLPTEVPSGRSFLAIQVDGRVATTSATCEGVEERRNGRTVEVRLAFEGESLRFT
                     VRRARGGDGSTAYPVGDDLRVRGSFFEGASGPLQGAVVLDASGRSGAVNLVRGDRSVS
                     GTWTCEA"
     gene            complement(1376..3166)
                     /locus_tag="VM638_03425"
     CDS             complement(1376..3166)
                     /locus_tag="VM638_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HVM11514.1"
                     /translation="MREGRGISRRELLRRAGLGLGAVSVGRLLGAGPAWAATGTGAAA
                     RAAEAAGVTEPLMLARVKPTSAAQAVALAAFDDTHRRFRDGSIEVLLWPGDLARLEQL
                     GIDHTITCRDVVARDASESEASSDAPALGLINMPGGDRTGYRQLADYENELKQLPRTY
                     PHLARLITFPERTLEGRLVHGIEIAANVGVVDGRPMAYIDGVHHAREWPAAEYPMIFA
                     HYLLENYGKDQRVTSLLNRCRVSILPVQNPDGFAYTRAGLENSNLSIVAGGQGAYWRK
                     NRRGVGQENEANGLNPTAFGVDPNRNYPFLWGATTGGAAGYVDPVPAYASTSPNPLDQ
                     TYYGRDALSEPETRNVAGYLLSRNVTGMITNHTYGRLVLRPWGHTNTPTVDETLMRTV
                     GAQMASAMGGYANQIGLGLYPTTGTTDDWYYAAASAIGYTFEHGTAFHPNYTSGENAV
                     RNFWPRSMNAFMILCEAAADPAKHCVVRGRAIEPVPGQEPKPVVVSLDAHKEFDTPMW
                     QQGATGIGPLLAQKTATREVLDLNSASDLQGVIEWHLNPTTRPLEAAAGRTETYRLRI
                     AAPGYRPKIQDIVLTRGEVLDLQDVVMERA"
     gene            complement(3309..>4318)
                     /locus_tag="VM638_03430"
     CDS             complement(3309..>4318)
                     /locus_tag="VM638_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVM11515.1"
                     /translation="REVNARLVAIESHAIRFAGEGREQEGLDLLVGPEYLELKRRMNH
                     RLVEAIDRMRVEEARADREGRARRTVGLSLALGSIPAVILVWLLLLRGVRRWSLDRRR
                     AEAQRREAEEERRRLLAHIVRAQEEERRRIAADIHDDSIQALTAVSLRIDVLRRTAPE
                     TIGLDHLAQTVDEAIRRLRSLMFELHPRVLDRDGLGPAIHESLRQASEESGFIFELDD
                     RLGIDLPMDVRVTGYRIVREAIANIRKHARASRVRVELTARDGSAFIVVADDGVGFSA
                     NGASDSPLHLGLSTMRERAELTGGRLDVDSLPGHGTTVRVAIPISGGEAAPERQAPRA
                     S"
BASE COUNT          609 a         1523 c         1529 g          657 t
ORIGIN      
        1 aaggccccgg cggagacgcc ctcgtcgacg atccccgccc agagacgctc gtaccgctcc
       61 cggagcgccc ggacctcctc gagccgggca ccggagaggg ctcgccactc gtgcaggaac
      121 actcgcgcct cgtcgatgga gcccgcgacc acgcggacgt gggctcggag cgcgcggcgc
      181 agcttctcct ccgggggaag ctccgccctc gcgacgggtt ccacccccgc gaggaactgc
      241 ccggccgcgc gcgtcacgat ctcgtggagg aggtcctcct tcgacgcgat gtgcgcgtag
      301 aggctccccg agagcatccc ggtctcctgc gcgatgtcgc gcacgctcgt cccgtggtag
      361 ccgcggtcgc tgaacaaccc gacggcggcg tcgaggatcg cctggcggcg tccgttcctc
      421 ggcatgtcgc ccagtatagc caggttagca agcgttcgct tggctaggcc tcgcaggtcc
      481 aggtgccgga gacggagcgg tccccccgca cgaggttcac ggcgccggat cggcccgaag
      541 cgtcgaggac gacggcaccc tgcagcggcc ccgaggcgcc ctcgaagaac gacccgcgga
      601 cccgaaggtc atcgccgacg ggataggcgg tggagccgtc cccgccgcgg gcgcgtcgga
      661 cggtgaaccg gaggctctcc ccctcgaagg cgagcctgac ctccaccgtt cggccgttgc
      721 gtcgttcctc gaccccctcg cacgtcgccg aggtggtggc gacccggccg tcgacctgga
      781 tcgccaggaa ggatcgcccg gagggaacct cggtgggcag atcggtgggc agaacgaccc
      841 ccggcaggtt cgtcgggatc gtcgcgggca cggtcggatc ggcacgggtc tcgctcgggt
      901 ccccgccggc ggagaggtac caggggatgc ccacgccgac gaacacgatg ccgaggagcc
      961 ccaggagccc cggcacgaac caggtccgga accgccccgc cagacgcgcc ttctgggggt
     1021 tggacggcag gtaccgcacc tcgaccatct cgccgggctg gtgcgaaggt gggttcgagc
     1081 cgagcttgtc ctggaactgg atctcctggc catccatcgc cgtgaagctg acgatggggt
     1141 ggtagacggt gccgccctcg ccccccgatc ccatcgcgag cccggtcacc gtgccgggga
     1201 cggtgaccgc gtcccgcagg aagcgcttcg tccgccacac gatgaacagc gcgagcagaa
     1261 cgaggaaccc acccacgccg atgaaggcga tcccgccgat ctggctctcg tccaccgaac
     1321 ccccctccgc aggccgggcg ccgatcgccc ggacgcgcgg atgctagcgg ttcggctagg
     1381 cgcgctccat cacgacgtcc tggaggtcga ggacctcacc gcgcgtgagc acgatgtcct
     1441 ggatcttcgg cctgtacccg ggcgctgcga tgcggaggcg gtaggtctcg gtcctgccgg
     1501 ctgcggcctc gaggggacgg gtcgtcgggt tgaggtgcca ctcgatcacg ccctggaggt
     1561 cggaagcgct gttgaggtcg agcacctccc gcgtcgcggt cttctgcgcg aggagcggtc
     1621 cgatccccgt cgcaccctgc tgccacatcg gagtgtcgaa ctccttgtgc gcatcgaggg
     1681 agacaacgac cggcttcggc tcctggccgg gcaccggttc gatcgcgcgg ccgcggacca
     1741 cgcagtgctt cgccgggtcc gccgccgcct cgcagaggat catgaacgcg ttcatcgagc
     1801 gcggccagaa gttccgcacg gcgttctcac cggaggtgta gttcgggtgg aaggcggtcc
     1861 cgtgctcgaa cgtgtatccg atcgcgctcg cggcggcgta gtaccagtcg tcggtcgtcc
     1921 ccgtcgtcgg gtagaggccc agtccgatct ggttcgcgta gccacccatc gccgacgcca
     1981 tctgggcgcc gaccgtgcgc atgagggtct cgtcgaccgt gggcgtgttc gtgtggcccc
     2041 acggccgcag cacgaggcgt ccgtacgtgt ggttcgtgat catgcccgtg acgttgcgcg
     2101 agaggaggta gcccgcgacg ttgcgggtct ccggctccga cagcgcatcc ctgccgtagt
     2161 aggtctggtc gagcggattg ggcgaggtgc tcgcgtacgc ggggacgggg tccacgtacc
     2221 cggccgcgcc gcccgtcgtc gctccccaga ggaaggggta gttccggttg gggtcgaccc
     2281 cgaaggccgt cgggttgaga ccgttcgcct cgttctcctg accgacgccg cgacggttct
     2341 tccgccagta cgcgccctgt ccacccgcaa cgatcgacag gttcgagttc tcgaggcccg
     2401 ctcgcgtgta ggcgaagccg tcggggttct ggaccggcag gatcgagacg cgacaccggt
     2461 tgagcaggct ggtaacccgc tggtccttgc cgtagttctc cagcaggtag tgggcgaaga
     2521 tcatcgggta ctcggcggcg ggccattccc gcgcgtgatg gacgccgtcg atgtaggcca
     2581 tgggtcgccc gtcgaccacg ccgacgttcg cggcgatctc gatgccgtgg acgaggcgcc
     2641 cctcgagcgt ccgctccggg aaggtgatga gccgcgcgag gtggggatac gtcctgggga
     2701 gctgcttcag ctcgttctcg tagtccgcga gctggcggta gcccgtccgg tccccgccgg
     2761 gcatgttgat caggccgagg gccggggcgt cggaggaggc ctcgctctcg gaggcgtccc
     2821 gagcgacgac gtctcggcag gtgatcgtgt ggtcgatccc gagctgctcg aggcgagcga
     2881 ggtcgcccgg ccacaggagc acctcgatcg agccgtcgcg gaagcgtcgg tgcgtgtcat
     2941 cgaatgcagc gagcgcgacg gcctgcgccg ccgaggtcgg cttcacgcgc gcgagcatca
     3001 gcggctccgt gacgccagct gcctccgccg ctcgagcggc cgccccggtt ccggtcgctg
     3061 cccaggccgg gccggctccg agcagccgtc cgaccgagac ggccccgagg ccgagcccgg
     3121 cacgccgaag gagctcccgc cgactgatcc ctcgaccttc cctcatcgtc accctccggt
     3181 tcgccgtgtc ccgtgcaggg aggcatcgct ccctctcagg ctctggatac gagggcgacc
     3241 ccgccgaatc ctttgtgcag gtgcacaggg tctccgccag gggccgtccg gagagcggcg
     3301 agtcagggct aggacgctcg cggggcctga cgttcgggag ccgcttcgcc gcccgagatc
     3361 gggatcgcca cgcgaacggt ggtgccgtgt cccggaaggc tgtcgacatc gagccgtccg
     3421 cccgtcagct ccgcgcgctc gcgcatcgtg gagagcccga ggtgcagcgg cgagtcgctc
     3481 gcgccgttcg cggagaagcc gacaccgtcg tccgccacga cgatgaacgc ggagccgtcg
     3541 cgagcggtga gctcgacacg gacgcggctc gctcgcgcat gcttgcggat gttcgcgatc
     3601 gcctcccgca cgatccggta gcccgtgacg cgcacatcca tcggaaggtc gatcccgaga
     3661 cggtcgtcga gctcgaagat gaacccgctc tcctcgctcg cctgccggag cgactcatgg
     3721 atcgcggggc cgaggccgtc gcggtcgagc acgcgcggat gcagctcgaa catcaggctg
     3781 cggagccgcc ggatcgcctc gtccacggtc tgagcgaggt ggtcgagccc gatcgtctcc
     3841 ggcgccgtcc gtcgcaggac gtcgatgcgg agggaaaccg cggtgagcgc ctggatcgaa
     3901 tcgtcatgga tgtcggcggc gatcctgcgt cgttcctcct cctgcgcgcg gacgatgtgc
     3961 gcgagcagcc gtcggcgctc ctcttcggcc tcccgccgct gcgcctcggc gcgacgccgg
     4021 tcgaggctcc agcgacggac cccgcgcagg agcagcagcc agaccaggat gacggcgggg
     4081 atgctcccca gagcgaggct gagtccgacg gtccggcgcg cacgtccctc tcgatcggca
     4141 cgggcttcct cgacgcgcat ccggtcgatc gcttcgacga ggcggtggtt catccgtcgc
     4201 ttcagctcca ggtactccgg gcccacgagg agatcgaggc cctcctgctc ccgcccctcc
     4261 ccggcgaagc ggatcgcgtg gctctcgatc gcgacgagac gggcgttgac ttcacggg
//
LOCUS       DATLGG010000122         5552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_55347, whole genome shotgun sequence.
ACCESSION   DATLGG010000122 DATLGG010000000
VERSION     DATLGG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55347"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VM638_03435"
     CDS             complement(<1..418)
                     /locus_tag="VM638_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HVM11516.1"
                     /translation="MPRRLFLLLAGLLSLGLGVASTGAIAMLNYIEGSLKQTHVEGLD
                     SPRASTGPRSDLEGQVLNVLVIGSDSREVLSDEKQVLDGDETLVEGQRSDTIILVHLD
                     PRRGKAVLVHFPRDLRVEIPGHGLDRINTAYNRGGPS"
     gene            507..2444
                     /locus_tag="VM638_03440"
     CDS             507..2444
                     /locus_tag="VM638_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006900895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVM11517.1"
                     /translation="MSDIVWRPSEEYLERANVTRLMRAHGIASYDELIRRSTEDIEWF
                     WQAAIDDLGIEFSRPFERLLDTSRGIAWSTWFAGGRINLAHNCVDRWAERTPEAVAVI
                     WEGEDGAVRRVTYRELREMADRLAHGLRSLGVEQGDAVGIFLPMAPETVAATLACAKL
                     GAVYLPIFSGYAADAVATRLADAEAKVLITADGSLRRGRPVPMRGIAEQAAARVPSVE
                     HILVWERLGGDAAWSDRVIPWSRLLEDQPSSFDTLELDPEHPLFIAYTSGTTGRPKGS
                     VHVHGGFTVKIAEEVAYQVDLHPDEILFWVTDLGWIMGPWEITGAGALGATVFLFEGA
                     PDFPEPDRLWSMIERHRITTLGISPTLIRALIPHGTEWVERHDLSSLRVIGSTGEPWN
                     PEPYHWTFEHVGGGRCPIINLSGGTEVGACFLSPHPVTELKTASLRGPALGMAVEVWG
                     PDGRPVAPGEVGELVCTKPWPSMTRGVWKDAQRYLDTYWSRWPEVWVHGDWATIDEDG
                     HWFLHGRSDDTMNIAGKRLGPAEVESALIEHAAVAESAAVGVPHEVKGEAIWCFVVTK
                     PGAARGPELAQELKEVVGKHLGKAFRPDRVLFVDELPKTRSAKILRRAVRATALGEEP
                     GDLSSLENPTAIEKIRAAVAS"
     gene            2481..3707
                     /locus_tag="VM638_03445"
     CDS             2481..3707
                     /locus_tag="VM638_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012942899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVM11518.1"
                     /translation="MDAYDVLLTIAGVAALGAALLPRMIAQHPVSFPMVYVALGMLLF
                     ALPLGFDPPHPLRERELTERLTELGVIVALTGAGLKLDRPVGWRRWAPTWRLLAITMP
                     LTIAGVFALGWWGLGLAPASALLLGACLAPTDPVLASDVQVAGPNTEEEDEVRFTLTS
                     EAGLNDALAFPFTNAAIAMALAGAAPAAWIGEWLLVDVLYKIVVGIACGWILGRALAL
                     LIFRTPSQTRLAETTEGITALAITLLAYGITELAAGYGFLAVFVAATTLRGAERHHEY
                     HRALHEFIESVERILIAALLVLLGGAIVGGLLQPLTWQAALVGLATVFIIRPATGLIA
                     LVGSVESVHERAAVAFFGIRGIGSLYYLAYGINEAPIQRPEVLWAFVSFVVLVSIVVH
                     GIAATPVMRHLDRRRG"
     gene            complement(3714..5216)
                     /locus_tag="VM638_03450"
     CDS             complement(3714..5216)
                     /locus_tag="VM638_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM11519.1"
                     /translation="MHRYGRRSLRLVGSIVGVLVVAAGGASQALPACVDQVVGVNERC
                     AAWTGAYNHPGGRGGNGEDIGRAVAASPDGTKVFVTGQSRDDTTLQDQATAAFQVGTG
                     EILWSKRVDAAAQFDTGSAIAVDPAGTRVFVTGRSVAFEGFDWLTTAHDASTGEILWT
                     RRYAGPKSVDDTSFGVVTSPDGETVYVAGTADDGAGIPGDAMVEAYDAATGERRWSAR
                     YDGGGYDGGLRVATAADGSLVYLLGTTRRLAGNDYLVAAFEAEDEERLGERVWAATYD
                     GAGLGDNVAGFALSGDGATIAVTGTSVGPQGNQDYATVAIDALTGARRWAVRYDSTRS
                     GNDSAAGVAITGSSVVVTGTGHEGVVCCTDYVTIAYDLATGAQRWMATENSAARFNDT
                     ATTISASAGQVFVSGYAVVPRGEIQPIFKIEPGTIMTASYDVATGERRWIAHHNQSGV
                     GSDVAVGSTMSPNGAALYVVGTFITSGVSLYPVAAAKPYAYDMGVVAYPV"
     gene            5362..>5552
                     /locus_tag="VM638_03455"
     CDS             5362..>5552
                     /locus_tag="VM638_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11520.1"
                     /translation="MPSRRLSSILRHRWAPDAAILAVAFVATLVAEWRTIVDPAVYPV
                     DATIHTYWMARFDDPALFAG"
BASE COUNT          738 a         1980 c         1993 g          841 t
ORIGIN      
        1 ggctcggccc gccccggttg tacgcggtgt tgatgcggtc gagcccgtgg ccggggatct
       61 cgacgcggag atcccggggg aaatgcacga gcacggcctt cccgcgccgc ggatcgaggt
      121 ggacgaggat gatcgtgtcc gaccgctggc cctcgacgag cgtctcgtcc ccatcgagca
      181 cctgcttctc gtcgctcagg acctcgcgcg aatcgcttcc gatcacgagc acgttcagga
      241 cctggccctc gaggtccgag cgaggtccgg tcgaggcgcg ggggctgtcg agaccctcga
      301 cgtgggtctg cttcagcgac ccctcgatgt agttcaacat cgcgatcgcg ccggtcgagg
      361 cgacccccaa cccgagcgac aggaggcccg ccagcagcag gaacaggcgt cggggcaccc
      421 tggagcgacg cgggcgccgg gaggtagggt tggacatcag gcaaagcgta ccgtcgcggc
      481 cgctggagcg tccggaagga gggggattga gcgacatcgt gtggcgcccg agcgaggagt
      541 acctcgagcg agcgaacgtc acccggctga tgcgcgcgca cgggatcgcg tcctacgacg
      601 agctgatccg ccgctcgacc gaggacatcg agtggttctg gcaggccgcg atcgacgacc
      661 tcggcatcga gttctcccgg ccgttcgagc ggctgctcga cacctcgcgc gggatcgcgt
      721 ggtcgacctg gttcgccggc ggacggatca acctcgcgca caactgcgtc gaccgctggg
      781 ccgagcggac cccggaggcc gtggcggtga tctgggaggg cgaggacgga gccgtccggc
      841 gcgtcaccta ccgcgagctg cgggagatgg ccgaccgctt ggcgcatggg ctgcggtcgc
      901 tcggcgtcga gcagggggac gcggtcggga tcttcctccc gatggcgccg gagacggtgg
      961 cggcgaccct cgcgtgcgcg aagctcggcg ccgtctacct cccgatcttc tccggctacg
     1021 cggccgacgc cgtcgcgacg cgtctcgccg atgccgaggc gaaggtcctc atcacggcgg
     1081 acgggtccct gcggcgcggg cgcccggtcc cgatgcgcgg catcgccgag caggcggcgg
     1141 cgcgcgtccc gtcggtcgaa cacatcctcg tctgggagcg gctggggggc gatgcggcgt
     1201 ggagcgaccg cgtgatcccg tggtcgcgct tgctcgagga tcagccgtcg agcttcgaca
     1261 ccctcgagct cgaccccgag catcctctct tcatcgccta cacgagcggg acgacgggcc
     1321 gccccaaggg ctcggtccac gtccacggcg gcttcaccgt caagatcgcg gaggaggtcg
     1381 cgtaccaggt ggacctccac ccggatgaga tcctgttctg ggtgacggac ctgggctgga
     1441 tcatgggccc gtgggagatc accggcgccg gcgcgctcgg cgcgaccgtg ttcctgttcg
     1501 aaggcgcccc cgacttcccc gagccagacc ggctctggtc gatgatcgaa cgccaccgca
     1561 tcacgacgct cgggatctcg ccgaccctga tccgcgcgct gatcccgcat ggcaccgagt
     1621 gggtcgagcg gcacgacctg tcgtcgctgc gggtgatcgg ctccaccggg gagccgtgga
     1681 accccgagcc gtaccactgg acgttcgagc acgtcggcgg tggacgctgc ccgatcatca
     1741 acctctccgg cgggacggag gtcggggcct gcttcctgtc gccgcacccg gtgaccgagc
     1801 tgaagaccgc gagcctgcgc gggccggcgc tcgggatggc ggtcgaggtc tggggtccgg
     1861 acggccgccc ggtcgcgccc ggcgaggtcg gcgagctcgt ctgcaccaag ccgtggccgt
     1921 cgatgacgcg cggcgtgtgg aaggacgcgc agcgctacct cgacacgtac tggtcgcgct
     1981 ggcccgaggt ctgggtgcac ggcgactggg cgacgatcga cgaggacggt cactggttcc
     2041 tgcacggccg ttcggacgac acgatgaaca tcgcgggcaa gcgtctcggg ccggcggagg
     2101 tcgagtcggc gctgatcgag cacgccgcgg tcgcggagtc ggcggcggtc ggcgtgccgc
     2161 acgaggtcaa gggtgaggcg atctggtgct tcgtcgtgac gaaacccggc gccgcgcgcg
     2221 gcccggagct tgcgcaggag ctcaaggagg tcgtcgggaa gcacctcggc aaggcgttcc
     2281 gtcccgaccg agtgctgttc gtcgacgagc tcccgaagac ccggtccgcg aagatcctgc
     2341 gccgggccgt ccgcgcgacc gcgctcggcg aagagcccgg cgacctatcc agcttggaga
     2401 acccgacggc gatcgagaag atccgagcgg ccgtcgcgag ctgagggtgc ccgggccgtc
     2461 gtgatgcatc ccccctcccc gtggacgcgt acgacgtcct cctgacgatc gccggggtgg
     2521 cggccctcgg ggcggcgctg ctcccgcgca tgatcgcgca gcaccccgtc tcgttcccga
     2581 tggtgtacgt cgccctgggg atgctgctct tcgcccttcc gctgggcttc gaccccccgc
     2641 acccgttacg ggaacgagag ctgacggagc gtctcacgga gctcggggtg atcgtcgcgc
     2701 tcaccggggc ggggctgaag ctcgaccggc cggtgggatg gagacgctgg gcgccgacgt
     2761 ggcggctgct ggcgatcacg atgcccctga cgatcgcggg cgtgttcgcg ctcgggtggt
     2821 gggggctcgg tctcgccccc gcgagcgcgc tgctgctcgg agcgtgcctc gccccgaccg
     2881 acccggtcct cgcgtccgac gtgcaggtcg ccgggccgaa caccgaggag gaggacgagg
     2941 ttcgcttcac gctcacctcc gaagcggggc tgaacgacgc cctcgcgttc ccgttcacga
     3001 acgcggcgat cgcgatggcg ctggccggcg ccgccccggc cgcgtggatc ggcgagtggc
     3061 tgctcgtcga cgtcctctac aagatcgtgg tcgggatcgc ctgcgggtgg atcctcggcc
     3121 gcgcgctcgc gctgctgatc ttccgcacgc cgtcccagac acgcctcgcc gagaccaccg
     3181 agggcatcac ggcgctggcg atcaccctgc tcgcctacgg catcacggag ctggcggcgg
     3241 ggtacggatt cctcgcggtc ttcgtcgcgg cgacgaccct ccggggcgcc gagcggcacc
     3301 acgagtacca ccgggcgctg cacgagttca tcgaatcggt cgagcggatc ctcatcgccg
     3361 ccctgctcgt cctgctcggc ggcgcgatcg tcgggggcct gctccagccg ctcacgtggc
     3421 aggccgcgct cgtggggctc gcgaccgtgt tcatcatccg gccggcgacg gggctgatcg
     3481 ccctcgtcgg gagcgtcgag tcggtgcacg agcgagcagc ggtcgcgttc ttcgggatcc
     3541 gcgggatcgg ctcgctctac tacctcgcat acggcatcaa cgaagcgccg atccagcggc
     3601 cggaggtgct ctgggccttc gtcagcttcg tggtgctcgt ctcgatcgtg gtgcacggca
     3661 tcgccgcgac gccggtcatg cgccacctcg accggcgtcg cggctgacgc gcgtcagacc
     3721 gggtaggcga cgacccccat gtcgtacgcg tagggcttcg cggccgcgac cggatacagg
     3781 gagacgccgc tcgtgatgaa cgtccccacg acgtagaggg ccgcaccgtt cgggctcatg
     3841 gtgcttccga ccgcgacgtc ggagccgacg ccgctctggt tgtggtgcgc gatccaccgc
     3901 cgctcgccgg tggccacgtc gtacgaggcg gtcatgatcg tcccgggctc gatcttgaag
     3961 atcggctgga tctcgccacg cgggacgacg gcgtagccgc tgacgaagac ctggccggcg
     4021 ctggccgaga tcgtcgtggc cgtgtcgttg aagcgggcgg cgctgttctc ggtcgccatc
     4081 catcgctggg ctccggtcgc gaggtcgtag gcgatcgtca cgtagtcggt gcagcagacg
     4141 accccttcgt gcccggtgcc ggtgacgacg acggaggagc ccgtgatggc gacgcccgcc
     4201 gcggagtcgt tcccgctgcg cgtgctgtcg tacctcacgg cccagcgacg cgcgcccgtg
     4261 agcgcgtcga tcgccaccgt tgcgtagtcc tggttgccct gtggcccgac ggaggttccg
     4321 gtcacggcga tcgtcgcgcc gtccccggag agggcgaacc cggcgacgtt gtccccgagg
     4381 ccagccccgt cgtacgtcgc ggcccacacc cgctcgccga gccgctcctc gtcctcggcc
     4441 tcgaacgcgg cgacgaggta gtcgttgccg gcgaggcgcc gcgtcgtccc aaggaggtag
     4501 accagggaac cgtcggccgc ggtcgcgacg cgcaggccgc cgtcgtagcc gccgccgtcg
     4561 taccgcgcgg accagcgccg ctcgccggtc gcggcgtcgt aggcctcgac catcgcgtcg
     4621 ccggggatcc cggcgccgtc gtcggccgtt cctgccacgt acacggtctc gccgtcggga
     4681 gaggtcacga cgccgaacga ggtgtcgtcg acgctcttcg ggcccgcgta ccgccgcgtc
     4741 cacaggatct cgccggtcga ggcatcgtgg gccgtcgtca gccagtcgaa gccctcgaag
     4801 gcgacgctgc gccccgtgac gaagacgcgt gtacccgcgg ggtccacggc gatcgccgag
     4861 cccgtgtcga actgggcagc ggcgtcgacg cgcttgctcc acaggatctc gccggtgccc
     4921 acctggaagg cggcggtcgc ctggtcctgg agggtcgtgt cgtcccggct ctgtccggtg
     4981 acgaagacct tcgtgccgtc cggggaggcg gccaccgcac gaccgatgtc ctcgccgttg
     5041 cccccacgcc cgcccggatg gttgtacgcg ccggtccacg cggcacagcg ctcgttgacg
     5101 ccgacgacct ggtcgacgca ggccggcagg gcctggctcg cgccgccggc ggccaccacg
     5161 aggaccccga cgatcgatcc gaccagccgc aggctcctgc ggccgtaccg atgcatgctc
     5221 acgttcgctc cctcctcgat cacgacgacg cgggcatctt cgacgggccg ggcatcccgt
     5281 cctttgtgca gctgcccaaa ccctccgccg ccgaccgccg tctccggccc gcgtatcctg
     5341 aggcctcccg acgaccgagc catgccgagc cgacgcctct cctcgatcct ccgccatcgc
     5401 tgggcgcccg acgccgcgat cctggccgtc gccttcgtcg ccaccctcgt ggcggagtgg
     5461 cggacgatcg tggatcccgc cgtctatccg gtggatgcga cgatccacac gtactggatg
     5521 gcgcggttcg acgaccccgc gctgttcgcg gg
//
LOCUS       DATLGG010000123        19116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_87169, whole genome shotgun sequence.
ACCESSION   DATLGG010000123 DATLGG010000000
VERSION     DATLGG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19116
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87169"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VM638_03460"
     CDS             <1..364
                     /locus_tag="VM638_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11521.1"
                     /translation="ARGPSGASTGALITDPKDLAEVEEEPTPAEEPKPAEEPTPAEEP
                     MPAQSPSVEVDQPPAGEPAGGEAAEGDAAEQEQPEREPGEAPAPGSEGEGAAEAAGTG
                     EAAEGDAAEQQQPEGGGS"
     gene            364..783
                     /gene="rplP"
                     /locus_tag="VM638_03465"
     CDS             364..783
                     /gene="rplP"
                     /locus_tag="VM638_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HVM11522.1"
                     /translation="MLMPKKVKHRKQHRGRMRGKAKGGTEIHFGDYALVAMEPGWITN
                     RQIEAARVAMTRHIRRGGKVWINIFPDKPVTKKPAETRMGSGKGNPEEWVAVVKPGRV
                     MFELAGVSEPVAREAMMRAAHKLAVRCKFATRMGGAA"
     gene            780..1013
                     /gene="rpmC"
                     /locus_tag="VM638_03470"
     CDS             780..1013
                     /gene="rpmC"
                     /locus_tag="VM638_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041323024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HVM11523.1"
                     /translation="MTRPSELRELSDQELLDRLESGKEELFNLRFQLATGQLDNPMRI
                     KQVRHEVARLLTIMRERQIEAAEGGLEEQEEVS"
     gene            1010..1267
                     /gene="rpsQ"
                     /locus_tag="VM638_03475"
     CDS             1010..1267
                     /gene="rpsQ"
                     /locus_tag="VM638_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HVM11524.1"
                     /translation="MSERGERKTRVGVVVSDAMDKTIVVRVDSQKTHSLYGKTMRRSA
                     RLKAHDEANDARVGDTVRVMETRPLSKTKRWRMVEVVERAK"
     gene            1277..1645
                     /gene="rplN"
                     /locus_tag="VM638_03480"
     CDS             1277..1645
                     /gene="rplN"
                     /locus_tag="VM638_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HVM11525.1"
                     /translation="MIQQESRAKVADNTGAKEVLCIRVLGGSARRYASVGDIIVGTVK
                     DALPGAPVKKGEIVRAVVVRTAKEKRRTDGSYIKFDDNAVVLINEQNNPRGTRIFGPV
                     ARELREKRFMKIISLAPEVL"
     gene            1648..1983
                     /gene="rplX"
                     /locus_tag="VM638_03485"
     CDS             1648..1983
                     /gene="rplX"
                     /locus_tag="VM638_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HVM11526.1"
                     /translation="MPGVHIKRDDEVQVIHGKDRGTRGRVVRVMPREGRLMVEGVARA
                     KKHTRTGGRRSKSGQQLQQGGILDVELFVDISNVQVVCKTCGQATRVGHRFEDGKKIR
                     VCRKCEADL"
     gene            1980..2561
                     /gene="rplE"
                     /locus_tag="VM638_03490"
     CDS             1980..2561
                     /gene="rplE"
                     /locus_tag="VM638_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HVM11527.1"
                     /translation="MSAATIPPRLRTRYREELRPQLQQELGLRNVMQVPGLEKIVINM
                     GVGEASKDAKVLDGAVADMTTIAGQKPVVTRARRSVAGFKIREGMPIGVKVTLRGARM
                     WEFLDRLLSVALPRIRDFRGLDPRGFDGRGNYTMGVTEQLIFPEIDYDKIDRVRGMDI
                     TVVTTAKDDDEGRALLRALGFPFRDMAGARDGG"
     gene            2571..2756
                     /locus_tag="VM638_03495"
     CDS             2571..2756
                     /locus_tag="VM638_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HVM11528.1"
                     /translation="MAKKGLIAKANRRPKFRVRGYTRCQRCGRSRAVFRRFMLCRLCF
                     RELAHAGEIPGVTKASW"
     gene            2764..3162
                     /gene="rpsH"
                     /locus_tag="VM638_03500"
     CDS             2764..3162
                     /gene="rpsH"
                     /locus_tag="VM638_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HVM11529.1"
                     /translation="MTMTDPIADMLTRIRNGSIAYKDEVQVPASRLKEEIAALLAREG
                     YVRGYRVEGEAPKRNLVIEMKYGADRERTISGLKRVSRPGRRVYVDRAHLPRVLGGLG
                     VAVLSTSQGVLTDRQAARRGVGGEVLCHVW"
     gene            3170..3715
                     /gene="rplF"
                     /locus_tag="VM638_03505"
     CDS             3170..3715
                     /gene="rplF"
                     /locus_tag="VM638_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HVM11530.1"
                     /translation="MSRIGKEPITIPDGVTVAVEGDRVTVTGPKGTLHQAISPDMQVT
                     VEDGTATVRRPTDERQHRALHGLTRTLVANMIEGVTKGFEKALEIQGVGYRAALQGRD
                     LELQLGFSHPVHVAAPDGIEFEVPAPTRIVVRGIDKQLVGAVAADIRKIRKPEPYKGK
                     GVRYEGEYVRKKAGKAAKGVG"
     gene            3712..4068
                     /gene="rplR"
                     /locus_tag="VM638_03510"
     CDS             3712..4068
                     /gene="rplR"
                     /locus_tag="VM638_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVM11531.1"
                     /translation="MNAAKKRADRVRRHRRVRKKIWGSAERPRLAVFRSNRHIYAQLV
                     DDAAGRTLAAASSLEADGRSDGGKRAAAKQVGSLLAQRAREAGIERAVFDRGGFLFHG
                     RVKELADAAREGGLTI"
     gene            4065..4664
                     /gene="rpsE"
                     /locus_tag="VM638_03515"
     CDS             4065..4664
                     /gene="rpsE"
                     /locus_tag="VM638_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HVM11532.1"
                     /translation="MRRYDAGEQKSPYEERVVSINRVAKVVKGGRRFSFAALVVVGDA
                     AGRVGIGYGKAKEVPAAIQKGVEDAKRSMFEVPMMGATIPHQVVGEDGAGVVLLKPAA
                     PGTGVIAGGPVRAVVECAGIKDILSKSLGSDNPINIVHATVTGLRSLRRPEEIAKLRD
                     RELHEVAPRPMLEAMAQARQRMTEKRSEAGLEGGGDGEA"
     gene            4651..4836
                     /gene="rpmD"
                     /locus_tag="VM638_03520"
     CDS             4651..4836
                     /gene="rpmD"
                     /locus_tag="VM638_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HVM11533.1"
                     /translation="MARLRITQIRSGVGRPEPQKRTLRALGLKRLRHAVVQEDRPEIR
                     GMIARVGHLVQVEEVDA"
     gene            4833..5270
                     /gene="rplO"
                     /locus_tag="VM638_03525"
     CDS             4833..5270
                     /gene="rplO"
                     /locus_tag="VM638_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVM11534.1"
                     /translation="MKLHHLRPAPGAKRKKKRVGRGIAAGQGKTAGRGTKGYLARNQK
                     RHGYEGGQMPLARRVPKEPGFTPRNREEWAVVNLKRLSSRFEEGATVTPEAMAEAGLV
                     RKRRPVKVLGYGDLDRALTVQAHGFSDTARQKIEQAGGSVQVL"
     gene            5330..6601
                     /gene="secY"
                     /locus_tag="VM638_03530"
     CDS             5330..6601
                     /gene="secY"
                     /locus_tag="VM638_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVM11535.1"
                     /translation="MLRAFVNAFKIPDLRKKIFFTIAIIAVYRIGSHLPIPNVDFNRL
                     AQEVEQQASTGVLGFINLFSGGALQRLALFALGIMPYITSSIIMQLLTVVIPKLEQWQ
                     KQGEIGTKKITQWTRYMTVTLALLQSTGLIFLFNNQFPGSIRQFTPSRVAFMILTLTA
                     GTALIMWLGELITQRGIGNGMSILIFTSIISTLPSEGASILAQGGNLAFAIIVALGLS
                     IIVAVVFMEQGQRRIPVQYAKRVVGRRMTSGGSTYIPLKVNQAGVIPIIFASSVMYFP
                     TLIASAVGGGFQNFVDRYLANQGSLVYIGIFGLLVVFFAYFYTAISFNPTDTADNIRK
                     YGGFIPGIRPGKPTSEYLERVLSRITLPGALFLATIALIPSIVFAFWLLRPDQFPFGG
                     ASILIVVGVALETMKQLESQLTMRHYEGFLR"
     gene            6605..7255
                     /locus_tag="VM638_03535"
     CDS             6605..7255
                     /locus_tag="VM638_03535"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HVM11536.1"
                     /translation="MRIVLLGPPGAGKGTQAHRLADYHDSRLIATGDMFRRHVREQTP
                     LGLKAKSYMDAGELVPDDVVIGMLLEELGKAANGFVLDGFPRTIPQAQALEEALAAVG
                     RPLQAVLKFKIPDGTAIARLAGRRTCSRCERTFNVELKPPKVRDICDACGGRLVQRDD
                     DREETVRHRLEVYHRDTEPLELFYWERGLLREVDAVGEVEDVTHRAIAVLDDVGDE"
     gene            7252..8004
                     /gene="map"
                     /locus_tag="VM638_03540"
     CDS             7252..8004
                     /gene="map"
                     /locus_tag="VM638_03540"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVM11537.1"
                     /translation="MIIRKSPQEIDRMRRAGRIVADTILRVLDHVRPGVTTGDLDRIA
                     EEQIRAAGAVPSFKGYRGFPASLCTSVNEEVVHGIPGTRVLEEGDLFSIDCGAIWEGY
                     HGDSAVSVFVGEPPTAEAEKLVRVTSDALDAGIAELRPGKRLSDVGHAVQQIVEGAGF
                     SVVREYVGHGIGQSLHEDPPIPNFGEPGRGPKIAPGLVVAIEPMVNLGGWETRVLADR
                     WTVVTADGSLSAHFEHTIAVTEDGPEVLTARS"
     gene            8094..10202
                     /locus_tag="VM638_03545"
     CDS             8094..10202
                     /locus_tag="VM638_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11538.1"
                     /translation="MTLIRRFLVLVLAAGLLAMPGQASAYPRPGRTTLISTGATGAQD
                     WSWLPDISHDGRFIVYESDSAGLVPGDTNGATDVFWHDRATGETRRASVAADGGQVRQ
                     GGNRPAVSDDGRYVGFYSYAADLIPGDTNPWADCFLKDMWTGAVELVSVAHDGGWTDG
                     GCFSIQMDSTGRSIVFESYGTNIVPGDTNGAADIFVRDREKGETRRVSVASDGTQADD
                     ASLVDPVISADGSVAAFYSTATNLTDDPKHSPGYIYAHDLRTGVTERVAKADDGTTAN
                     SGSIFKAISGDGRFVAFASGSTNLTPNDTNVSSDIFVHDLLTKKTRRVNVTSAGAEAE
                     ARTLAYTPSLSHDGRFVSFVTRAANLTEGDTNTKDDVFVHDLLTGETEMISVDLAGGP
                     SGESVTGRYNPNEITGTSVISGDGRHVVFASQARDLVAEATTAWRSIFVRDRGPSPGV
                     QDLRLEGSDGTTSVRGTATFGGSVVAAAADPADDAEPPGGDVGAEIVGAELVTRIERE
                     DLLVRIRLESLPGVRAPVLDESRGSFVPSVAGAPVVTYGFRFLAGSSAYEARAVSAPS
                     ADPATQSFSLHRCAETCERVRGLGGGIGVLAPEVTIAIPRAELAPPGAPLELSEVTAF
                     AQVGLAEARVPTDAVALGNPVLDEPRVALVVEDAQGERRVVEASLEAGRFAATIGDDE
                     RVEAVAACLGARCETLRPPA"
     gene            10331..10444
                     /gene="rpmJ"
                     /locus_tag="VM638_03550"
     CDS             10331..10444
                     /gene="rpmJ"
                     /locus_tag="VM638_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVM11539.1"
                     /translation="MKVRPSVKKMCEKCKVIRRRGRVMVICSNPRHKQRQG"
     gene            10452..10823
                     /gene="rpsM"
                     /locus_tag="VM638_03555"
     CDS             10452..10823
                     /gene="rpsM"
                     /locus_tag="VM638_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009199218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVM11540.1"
                     /translation="MARIAGVDLPRDKRVEVALTYIYGIGRTKASETVRRTGLDQATR
                     VRDLSEDEVVRIRDWIDQNYKVEGDLRRDVAQDIKRKIEISAYQGLRHRRSLPVHGQR
                     THTNARTRKGPKKTVGVKRKK"
     gene            10824..11213
                     /gene="rpsK"
                     /locus_tag="VM638_03560"
     CDS             10824..11213
                     /gene="rpsK"
                     /locus_tag="VM638_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HVM11541.1"
                     /translation="MAKPKKKVRKKEKKNVTHGAAHIKSTFNNTIVTITDPDGNTLAW
                     ASAGNVGFKGSRKSTPFAAQLAAEAAARRAQEHGLRKVDVFVKGPGSGRETAIRSLAA
                     AGLEILAIQDVTPVPHNGCRPKKRRRV"
     gene            11214..11840
                     /gene="rpsD"
                     /locus_tag="VM638_03565"
     CDS             11214..11840
                     /gene="rpsD"
                     /locus_tag="VM638_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HVM11542.1"
                     /translation="MARYNDADCKLCRRERMKLYLKGTKCETPKCPIERRPYPPGQHG
                     RARIKESEYLMQLREKQKARRIYGVMERQFRNYYESAARQRGITGENLLRLLETRLDN
                     VVYRAGFATSRDQARQLVLHRHFQVNGKIVNIPSYRVRPGDVVEVREKSRRLIPVQEA
                     VELAEGKIIPPWLDVNKGQLSVRVADLPQRVMIDTPVQEQLIVELYSK"
     gene            11922..12854
                     /locus_tag="VM638_03570"
     CDS             11922..12854
                     /locus_tag="VM638_03570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HVM11543.1"
                     /translation="MLIVARPRIQEETVNDRRSRFIIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVASVKIEGVLHEFSTVPKVTEDVTDIILNIKEMVLRADVDEPATIYLKAKGA
                     AEVTAGDIAPPAGVEILNPELHIATLAKGGSLEMEMVVERGVGYRSAEQNKKPRDPIG
                     VIPIDSMFSPVRRVSYSVEATRVEQMTDRDKLILDVETNGAISPREAVASAGTTLVEL
                     SGLFSELSEAAGLSLGPAEGEAQPSGELALPIEELTFSVRSYNCLKREGVNTVADLVQ
                     KSEQELMDIRNFGQKSIDEVKAKLEELGLSLREE"
     gene            12856..13209
                     /gene="rplQ"
                     /locus_tag="VM638_03575"
                     /pseudo
     CDS             12856..13209
                     /gene="rplQ"
                     /locus_tag="VM638_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            13235..13966
                     /gene="truA"
                     /locus_tag="VM638_03580"
     CDS             13235..13966
                     /gene="truA"
                     /locus_tag="VM638_03580"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVM11544.1"
                     /translation="MVLAYDGTGFRGWARQPGQRTVQGELERALERVLGRVPRIAVAG
                     RTDAGVHAAGQVVSFAADADPQRIGRAVNGMLAPEVAVLQAGRAPAGFDARRSATGRA
                     YRYRLRRAGTPDPFTARYVWHRPGEYRVGRMRRAARLLEGEHDFTSFSRRAPGVPPVR
                     RLQRLSVAVVGEELVIGATADGFLHQMVRSLVGTLVAVGEGKMEAEAMTEVLEARSRA
                     AAGPVAPPHGLTLVRVRYGVLGTER"
     gene            13963..14466
                     /gene="rplM"
                     /locus_tag="VM638_03585"
     CDS             13963..14466
                     /gene="rplM"
                     /locus_tag="VM638_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVM11545.1"
                     /translation="MKTYSPKAADITRGWYLVDAEGVVLGRLASEVAKILRGKHKPTF
                     APHVDGGDHVVVINAAKVSLSGGKESKKMAYRHSQYPGGLRAVPYERLLAERPERAVE
                     KAIKGMLPKNRLGRQMGKKLRVYAGPDHQQQAQQPVPLKVGDVPPAVPRLPGAGTAEN
                     GKESSDG"
     gene            14492..14860
                     /gene="rpsI"
                     /locus_tag="VM638_03590"
                     /pseudo
     CDS             14492..14860
                     /gene="rpsI"
                     /locus_tag="VM638_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            14874..16196
                     /gene="glmM"
                     /locus_tag="VM638_03595"
     CDS             14874..16196
                     /gene="glmM"
                     /locus_tag="VM638_03595"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HVM11546.1"
                     /translation="MFGTDGIRGIANRDLTPELAFDLGRAAAAVLGGDGRARFAIGRD
                     TRGSGEMLEAALAAGICSMGGDVLRLGVTPTPAVAFLTVELGAGGGVVISASHNPAEY
                     NGIKLFARDGTKLPDELEAEVERLLQDRPVDRPEGGGIGRIAEAPEAGDRYLAHVIDA
                     ARGSLEGMRVVVDCANGAASELAPTALRRLGAEVLPINDRPDGTNINAGCGATHPEVL
                     AAEVRRVGADAGVAHDGDADRAIFSDADGTIVDGDHVLAAEAIAMREEGSLAGNAVVA
                     TVMANLGFRRAMEAAGIGVVETKVGDRYVLEEMRRIGASLGGEQSGHIVFLDRATTGD
                     GLLTAVRFLSLARRRGVGLSELASVVEKYPQELLGVEAPDPGGLAADPAVIEAVRAAE
                     ASLGGSGRVLVRPSGTEPLLRIMVEAEELEQARKHAEAIADAARSAAP"
     gene            16240..18063
                     /gene="glmS"
                     /locus_tag="VM638_03600"
     CDS             16240..18063
                     /gene="glmS"
                     /locus_tag="VM638_03600"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HVM11547.1"
                     /translation="MCGIVGYVGPDEALPIVLEGLRRLEYRGYDSAGVAVLDGGLDVV
                     KRAGKLEVLDKELAQQGMPEGQVGMGHTRWATHGQPTDRNAHPHTDCRDRVAVIHNGI
                     IENFHVLKDRLEADGHEFRSDTDTEVVAHLIEERLDGDLAAAVRSAVAELEGAFALVA
                     LSTEEPDVVVGVRVSSPMVVGLGDGENLLASDVPALIDRTRRVVPLSDGQVVELRAGS
                     VRITDLDGREVSVEPMEIDWDLRRAEKEGFEDFMLKEIHEQPTAIRDTMRGRTREGRL
                     VLDELRMSDEDIRAVDKVFVVACGTAYHAGLVAKYAIEHWARLPVEIEIASEFRYRDP
                     VLDRNTLTLAVSQSGETIDTLEALRHAKRQHSWVLAITNTVGSSVARESDAVLYTHAG
                     PEIGVAATKTFATQMVALHLVALYLAQVRGVMYPREIAEVLDRMKRLPDQIATTVRLD
                     EQMRGLAQRYQDARDVLFIGRHTGYPSALEGALKLKEISYIHAEGYPAGELKHGPIAL
                     VEEGVPVVAVATECHVYPKMLSNIQEVRARGAEVVAIATEGDRDIEKHATHVVTVPRT
                     HELLSPVVVAVPLQLLAYHVAKLRGCDVDQPRNLAKSVTVE"
     gene            18070..18450
                     /locus_tag="VM638_03605"
     CDS             18070..18450
                     /locus_tag="VM638_03605"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF000832.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HVM11548.1"
                     /translation="MEILGIGVDVVDVGRIRRMLERHRRFRERVFTPDEVAYCETKAN
                     RAERYAARWAAREATIKALGGVRGLRYHDISVGRHRSGAPRIVLSGSARARAADRGVR
                     DVLVSFSHERDTTVAFCIAVGRAP"
     gene            18447..>19116
                     /locus_tag="VM638_03610"
     CDS             18447..>19116
                     /locus_tag="VM638_03610"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HVM11549.1"
                     /translation="MRPVLGAAQAKELDAASQERGVPADVLMERAGHALAREAVSIAG
                     GAAGRRVVVVAGTGNNGGDGLVAARRLRGRGAGVSVLLVGDRSRLSPLAEANLGRIHD
                     VPVWPFSSSTIERALDRADVAIDAIFGVGFRGSAEGDAAAAIAALNASPVPVVAADVP
                     SGVDADLGAVRGPAVLATRTVTFGAPKTGIILFPGAAHAGEVAVADIGFPPDLIRSDV
                     LLVDE"
BASE COUNT         3046 a         6472 c         7022 g         2576 t
ORIGIN      
        1 ggcgcgtggt ccgagcgggg cctcgaccgg agccctgatc accgacccga aggatctcgc
       61 cgaggtcgag gaagagccga cgcccgctga ggagcccaag ccggccgaag agccgacgcc
      121 cgctgaggag ccgatgccgg cgcagtcacc gtccgtcgag gtcgaccagc ctccggccgg
      181 tgagcctgcc gggggcgagg ccgctgaggg cgacgccgcc gagcaggaac agccggagcg
      241 ggagccgggc gaagccccgg cccccgggtc cgaaggtgag ggtgccgccg aggccgccgg
      301 gaccggcgag gcggctgagg gcgacgccgc cgagcagcag cagcccgagg gaggtggctc
      361 ctgatgctga tgccgaagaa ggtcaagcac cgcaagcagc accgcgggcg catgcgcggc
      421 aaggcgaagg gtggcaccga gatccacttc ggtgactacg cgctcgtcgc gatggagccg
      481 ggctggatca cgaaccggca gatcgaggcc gcccgtgtcg cgatgacgag gcacatccgt
      541 cgcggcggga aggtctggat caacatcttc cccgacaagc cggtgaccaa gaagccggcc
      601 gagacgcgga tgggctccgg gaagggcaac cccgaggagt gggtcgcggt cgtcaagccc
      661 ggccgcgtca tgttcgagct cgcgggcgtg tccgagcccg tcgcccgaga ggcgatgatg
      721 cgcgccgcgc acaagctcgc cgttcgctgc aagttcgcga cgcgcatggg aggtgcggcg
      781 tgacgcggcc gtccgagctc cgcgagctct ccgaccagga gctgctcgac cgcctcgagt
      841 cggggaagga ggagctgttc aacctccgct tccagctcgc caccggccag ctcgacaacc
      901 cgatgcggat caagcaggtc cgtcacgagg tcgcgcggct gctcacgatc atgcgcgagc
      961 gccagatcga ggcggccgaa ggcgggctcg aggagcagga ggaggtctcg tgagcgagcg
     1021 cggcgagcgc aagacccggg tcggcgtcgt cgtctccgac gcgatggaca agaccatcgt
     1081 cgtgcgcgtc gactcccaga agacgcactc cctctacggg aagacgatgc gtcggtccgc
     1141 ccggctcaag gcgcacgacg aggcgaacga cgcgcgggtc ggggataccg tgcgggtgat
     1201 ggagacgcgg ccgctgtcga agacgaagcg gtggcgcatg gtcgaggtcg tcgagcgggc
     1261 gaagtaggag cgagacgtga tccagcagga gtcgcgggcg aaggtcgccg acaacaccgg
     1321 ggcgaaggag gtcctctgca tccgggtgct cggcgggtcg gcccgccggt acgcgagcgt
     1381 cggcgacatc atcgtcggga ccgtgaagga cgcgcttccc ggcgcgccgg tgaagaaggg
     1441 tgagatcgtc cgcgccgtcg tcgtgcggac ggcgaaggag aagcgccgca ccgacggctc
     1501 ctacatcaag ttcgacgaca acgcggtcgt cctgatcaac gagcagaaca accctcgggg
     1561 gacccggatc ttcggaccgg tcgcccggga gctgagggag aagcggttca tgaagatcat
     1621 ctcgctggcg ccggaggtgc tgtagccgtg ccgggtgttc acatcaagcg tgacgacgag
     1681 gtccaggtga tccacggcaa ggaccgcggg acgcgcgggc gcgtcgtgcg ggtcatgccg
     1741 cgcgagggcc gcctgatggt cgagggtgtc gcccgcgcga agaagcacac gcgcaccggc
     1801 ggccgccgct cgaagagcgg ccagcagctc cagcaggggg ggatcctcga cgtcgagctc
     1861 ttcgtcgaca tctcgaacgt ccaagtggtc tgcaagacct gtggtcaggc gacgcgggtc
     1921 gggcaccggt tcgaggacgg caagaagatc cgcgtgtgcc ggaagtgcga ggcggacctg
     1981 tgagcgccgc gacgatcccg ccccgtctgc gcacccgcta ccgggaggag ctccggccgc
     2041 agctccagca ggagctcggc ctgcgcaacg tcatgcaggt ccccgggctc gagaagatcg
     2101 tgatcaacat gggggtcggc gaggcctcga aggacgcgaa ggtgctcgac ggcgcggtcg
     2161 ccgacatgac gacgatcgcg gggcagaagc cggtcgtgac ccgtgcccgc cgctcggtgg
     2221 cgggcttcaa gatccgcgag ggcatgccga tcggcgtcaa ggtcaccctc cggggcgcgc
     2281 ggatgtggga gttcctcgac cggctcctgt cggtcgccct cccgcggatc cgggacttcc
     2341 gcggactcga cccgcgcggg ttcgacggac ggggcaacta cacgatgggc gtgacggagc
     2401 agctgatctt ccccgagatc gactacgaca agatcgaccg ggtgcgcggg atggacatca
     2461 cggtcgtgac gacggcgaag gatgacgacg agggacgtgc cctcctgcgg gcgctggggt
     2521 tcccgttccg ggacatggcc ggcgcgcggg acgggggata ggtgaggtag atggcgaaga
     2581 agggactgat cgcgaaggcg aaccggcgac cgaagttccg cgtgcgcgga tacacgcgct
     2641 gccagcggtg cggacgctcg cgtgcggtgt tccgccggtt catgctctgt cggctctgct
     2701 tccgggagct cgcgcacgcg ggcgagatcc cgggcgtgac gaaggcctcg tggtgatgca
     2761 gcgatgacga tgaccgaccc gatcgccgac atgttgaccc ggatccgcaa cgggagcatc
     2821 gcctacaagg acgaggtcca ggtcccggcg tcgcggctga aggaagagat cgccgcgttg
     2881 ctcgcgcgcg aggggtacgt gcgcggctac cgcgtcgagg gtgaggcgcc gaagcgcaac
     2941 ctcgtgatcg agatgaagta cggagccgac cgggagcgca ccatcagcgg cctgaagcgt
     3001 gtgtcgcggc cgggacgccg ggtgtacgtc gaccgggccc acctcccgcg ggtcctcggc
     3061 ggcctcggcg tcgcggtcct ctccacgtcc cagggtgtgc tgacggaccg ccaggccgcg
     3121 cgacggggcg tcggcggcga agtgctgtgc cacgtctggt gaggatgaga tgagcaggat
     3181 cggcaaggaa cccatcacga tccccgacgg cgtgaccgtc gcggtcgagg gcgaccgtgt
     3241 gacggtgacc ggcccgaagg ggacgctgca ccaggcgatc agcccggaca tgcaggtcac
     3301 ggtcgaggac gggaccgcca ccgtgcggcg tcccacggac gagcgtcagc accgcgcgct
     3361 ccacggcctc acacggacgc tggtggccaa catgatcgag ggcgtgacga aggggttcga
     3421 gaaggcgctc gagatccagg gcgtcgggta ccgcgccgcc ctccagggac gcgatctcga
     3481 gctccagctc gggttctcgc atccggtcca cgtcgcggcc cccgacggga tcgagttcga
     3541 ggttccggcg ccgaccagga tcgtcgtgcg cgggatcgac aagcagctcg tgggtgccgt
     3601 cgcggcggac atccgcaaga tccggaagcc ggagccgtac aagggcaagg gcgtccgcta
     3661 cgaaggggag tacgtccgga agaaggccgg caaggccgcg aagggggtcg gatgaacgcc
     3721 gcgaagaagc gcgccgaccg cgttcggcgc caccgccggg ttcggaagaa gatctgggga
     3781 tccgccgagc ggccgcggct cgccgtgttc cgctcgaacc gccacatcta cgcgcagctc
     3841 gtcgacgatg ccgcgggtcg caccctggcg gccgcctcct cgctcgaggc tgacggccgc
     3901 tcggacggcg gcaagcgtgc cgccgcgaag caggtggggt cgctgctcgc gcagcgcgcg
     3961 cgtgaggccg ggatcgaacg ggcggtcttc gaccgcggcg ggttcctctt ccacggccgc
     4021 gtgaaggagc tcgcggatgc ggctcgcgaa ggaggcttga cgatttgagg cgctacgacg
     4081 caggagagca gaagagtccc tacgaggagc gggtggtctc gatcaaccgc gtggcgaagg
     4141 tcgtgaaggg tggacgccgc ttctcgttcg cggccctcgt cgtggtcggc gacgccgccg
     4201 gccgggtcgg gatcggctac gggaaggcga aggaggtccc cgcggccatc cagaagggcg
     4261 tcgaggacgc caagcgctcg atgttcgagg ttccgatgat gggagccacg atcccgcatc
     4321 aggtggtcgg ggaggacggc gccggcgtcg tcttgctcaa gccggccgct cccggtacgg
     4381 gcgtgatcgc cggcgggccc gttcgcgccg tcgtggagtg cgccgggatc aaggacatcc
     4441 tgtcgaagtc cctcgggtcg gacaacccga tcaacatcgt gcacgcgacg gtcaccggcc
     4501 tgcggtcgct gcggcggccg gaggagatcg cgaagctccg cgaccgcgag ctccacgagg
     4561 tcgcgccgag gccgatgctc gaggcgatgg cgcaggcgcg gcagcggatg accgagaagc
     4621 gctccgaggc cgggctcgag ggaggcggcg atggcgaggc ttaggatcac ccagatccgc
     4681 agcggcgtcg gcaggcccga accgcagaag cggacgctcc gcgcgctggg gctgaagcgg
     4741 ttgcgccacg cggtcgtcca agaggaccgg ccggagatcc gcggcatgat cgcccgcgtc
     4801 gggcacctcg tccaggtcga ggaggtggac gcgtgaagct gcatcacctg cgccccgcgc
     4861 cgggcgcgaa gcgaaagaag aagcgggtcg gccgcggcat cgccgccggc caggggaaga
     4921 cggccggacg gggaacgaag ggctacctcg cgcgcaatca gaagcgccac gggtacgagg
     4981 gcggtcagat gccgctcgcc cgacgggttc ccaaggagcc gggcttcacc ccgcggaacc
     5041 gcgaggagtg ggcggtggtc aacctcaagc ggctgtcgtc acgcttcgag gagggggcga
     5101 ccgtcacgcc ggaggccatg gccgaggcgg ggctcgtccg caagcgccgc ccggtgaagg
     5161 tgctcggcta cggcgacctc gaccgggccc tcacggtgca ggcgcacggg ttctccgaca
     5221 cggctcggca gaagatcgaa caggcagggg gcagcgtcca ggtcctctga ccgtgggcta
     5281 gactaagcgt ccgccgccgg ctcccggcgg gggaggagga gcaggagcgg tgcttcgagc
     5341 gttcgtcaac gcgttcaaga tcccggatct tcgcaagaag atcttcttca cgatcgcgat
     5401 catcgccgtg taccggatcg ggtcgcacct cccgatcccg aacgtcgact tcaaccggct
     5461 ggcccaggag gtcgagcagc aggcgagcac cggcgtcctc ggcttcatca acctcttctc
     5521 gggaggagcc ctccagcgcc tcgcgctgtt cgcgctcggg atcatgccgt acatcaccag
     5581 ctcgatcatc atgcagctcc tcaccgtcgt gatcccgaag ctcgagcagt ggcagaagca
     5641 gggtgagatc gggacgaaga agatcaccca gtggacccgc tacatgacgg taacgctggc
     5701 cctcctccag tcgacgggtc tgatcttcct gttcaacaac cagttccccg gctcgatccg
     5761 gcagttcacc ccatcccgcg tggcgttcat gatcctcacg ctcaccgccg ggacggcgct
     5821 gatcatgtgg ctgggtgagc tcatcacgca gcgcgggatc ggcaacggga tgtcgatcct
     5881 gatcttcacg agcatcatct cgacgctgcc gtcggaagga gcgagcatcc tcgcgcaggg
     5941 cgggaacctg gcgttcgcga tcatcgtcgc gctcggcctc tcgatcatcg tggccgtggt
     6001 cttcatggag cagggacagc ggcggatccc ggtgcagtac gcgaagcgcg tcgtggggcg
     6061 gcggatgacc tccggcggaa gcacgtacat ccccctgaag gtcaaccagg ccggcgtcat
     6121 cccgatcatc ttcgcgtcgt cggtgatgta cttcccgacg ctgatcgcct ccgccgtcgg
     6181 gggcgggttc cagaacttcg tcgaccgcta cctcgcgaac caggggagcc tcgtctacat
     6241 cgggatcttc gggctcctgg tcgtcttctt cgcgtacttc tacacggcga tctcgttcaa
     6301 cccgaccgac accgccgaca acatccgcaa gtacggcggg ttcatccccg ggatccggcc
     6361 cgggaagccg acgtcggagt acctcgagcg ggtgctttcc cggatcacgc tgccgggcgc
     6421 gctcttcctc gcgacgatcg cgctcatccc gtcgatcgtc ttcgcgttct ggctcctgcg
     6481 gccggatcag ttccccttcg ggggagcctc gatcctcatc gtggtcggtg tcgcgctcga
     6541 gacgatgaag cagctcgagt cccagctcac gatgcggcac tacgagggct tcctgaggta
     6601 gccgatgcgc atcgtgctgc tgggtccgcc gggagcgggg aaggggacgc aggctcaccg
     6661 tctggccgac taccacgaca gccggctgat cgcgaccggt gacatgttcc gccggcacgt
     6721 ccgggagcag acaccgctcg gcctcaaggc gaagtcgtac atggacgcgg gcgagctcgt
     6781 gcccgacgac gtggtgatcg ggatgctcct cgaggagctt gggaaggcgg ccaacggctt
     6841 cgtgctcgac ggcttccctc ggacgatccc ccaggcgcag gcgctcgagg aagcgctcgc
     6901 cgccgtcgga cgcccgctcc aggcggtgct gaagttcaag atccccgacg ggaccgcgat
     6961 cgcccggctc gcgggtcgcc gcacctgctc gcggtgcgag cggacgttca acgtcgagct
     7021 gaagccgccg aaggtccgcg acatctgcga cgcgtgcggt gggaggctcg tccagcgcga
     7081 tgacgaccgg gaagagacgg ttcggcaccg tctcgaggtc taccaccgcg acaccgagcc
     7141 gctcgagctc ttctattggg agcggggtct gctgcgcgag gtggatgccg tcggagaggt
     7201 cgaggacgtc acccaccgcg ccatcgcggt gctcgacgac gtcggcgacg agtgatcatc
     7261 cgcaagtccc cgcaggagat cgaccggatg cgccgggccg ggcgcatcgt cgccgacacg
     7321 atcctgcggg tcctcgacca cgtccgcccg ggcgtgacga cgggggacct cgaccgcatc
     7381 gccgaggagc agatccgcgc ggccggggcc gttccgtcct tcaaggggta ccgcggcttc
     7441 cccgcctcgc tctgcacctc ggtgaacgag gaggtcgtcc acggcatccc gggcactcgc
     7501 gtgctggagg aaggcgacct cttctcgatc gactgcggcg cgatctggga ggggtaccac
     7561 ggcgactccg ccgtctcggt cttcgtgggt gagccgccca ccgccgaagc ggagaagctc
     7621 gtccgggtga cgagcgacgc cctcgatgcc gggatcgccg agctccgccc gggcaagcgg
     7681 ctctccgacg tcggccacgc ggtgcagcag atcgtcgagg gagccgggtt ctccgtcgtg
     7741 cgcgaatacg tcgggcacgg gatcggccag agcctccacg aggacccacc gatccccaac
     7801 ttcggcgagc cgggccgagg gccgaagatc gcgccggggc tcgtcgtcgc gatcgagccg
     7861 atggtgaacc tcggcggctg ggagacacgc gtcctcgcgg accgctggac cgtggtgacc
     7921 gccgacggca gcctctccgc gcacttcgag cacacgatcg ccgtcaccga agacggtccg
     7981 gaggtcctga cggcacgcag ctgagggcgg tggcggacga accgccgcct gctgcggcat
     8041 ccccttcgcc cggtcggcag gagcgatgcc ctccggccgc gaaggaaccg gcgatgacgc
     8101 tcatccgacg cttcctcgtc ctcgtcctcg ccgctggtct cctcgcaatg cccgggcagg
     8161 cgtccgcgta cccacgaccc ggccggacca cgctcatctc cacgggcgcg accggcgccc
     8221 aggattggtc ctggctaccg gacatcagcc acgacggccg cttcatcgtg tacgagagcg
     8281 actccgccgg cctcgtaccc ggagacacga acggcgcgac cgacgtcttc tggcacgacc
     8341 gggccacggg cgagacccgt cgggcgagcg tcgccgcgga cgggggtcag gtccgtcagg
     8401 gggggaaccg gccggcggtg agcgacgacg ggaggtacgt cggcttctac agctacgcgg
     8461 cggacctcat ccccggcgat acgaacccgt gggccgactg cttcctgaag gacatgtgga
     8521 ccggcgcggt cgagctcgtc tcggtggcgc acgacggggg ctggacggac ggcggctgct
     8581 tctccatcca gatggattcc accggccgct ccatcgtgtt cgagagctac ggcacgaaca
     8641 tcgttcccgg cgacacgaac ggtgccgcgg acatcttcgt gcgggaccgg gagaaggggg
     8701 agacgcggcg ggtgtccgtc gccagcgacg ggacccaggc ggacgacgcg tcgttggtcg
     8761 atccggtgat cagcgccgac ggctccgtcg ccgcgttcta cagcaccgcg accaacctca
     8821 ccgacgaccc gaagcactcg cccggctaca tctacgccca cgacctccgg acgggcgtca
     8881 ccgagcgcgt cgcgaaggcg gatgacggaa cgaccgcgaa ctccggctcg atcttcaagg
     8941 cgatcagcgg cgacggacgg ttcgtcgcct tcgcctccgg cagcacgaac ctcacgccga
     9001 acgacacgaa cgtgagcagc gacatcttcg tccacgacct cctcacgaag aagacgcggc
     9061 gggtcaacgt gaccagcgcc ggcgccgagg cggaggcccg aaccctggcg tacacgccgt
     9121 cgctctctca cgacggacgc ttcgtctcgt tcgtcacgag ggcggccaac ctcaccgagg
     9181 gggacacgaa cacgaaggac gacgtcttcg tccacgacct cctcaccggg gagacggaga
     9241 tgatctcggt cgacctcgcg gggggtccct ccggggagtc cgtgaccggg cggtacaacc
     9301 ccaacgagat caccggcacc tcggtcatct ccggcgatgg gcgccatgtg gtcttcgcaa
     9361 gccaggcgag ggatctcgtg gcggaggcga cgacggcctg gcggagcatc ttcgtccgtg
     9421 accgcggccc gtcgcccggg gtgcaggatc tccgcctcga agggtcggac ggcactacct
     9481 cggtccgggg aacggccacc ttcggcggat ccgtcgtcgc ggccgccgcc gatcccgcgg
     9541 acgacgccga gccgcccggc ggggacgtcg gggccgagat cgtaggggcc gagctggtga
     9601 cgcgcatcga gcgtgaggat ctcctcgtcc gcatccgcct cgagtcgctc ccgggggtga
     9661 gggcaccggt tctcgacgag agtcggggct ccttcgtccc gagcgtggcc ggagcgcccg
     9721 tcgtcacgta cgggttccgc ttcctggcgg gctcttcggc gtacgaggcg cgcgccgtgt
     9781 ccgccccctc ggccgacccg gccacccagt cgttctccct ccaccgctgc gcggagacgt
     9841 gcgaacgcgt acggggcctc ggcgggggga tcggcgtcct cgccccggag gtcacgatcg
     9901 cgatcccgcg agccgagctc gccccccctg gcgccccgct ggagctctcc gaggtcaccg
     9961 cgttcgcgca ggtgggcctg gccgaggcga gggttccgac cgacgccgtg gccctcggga
    10021 accccgtgct cgacgagccc agggtggctc tggtcgtgga ggacgcacag ggtgagcggc
    10081 gggtcgtcga ggcctcgctc gaggccggcc gcttcgccgc gacgatcggc gatgacgagc
    10141 gggtcgaagc ggtggcggcc tgcctcgggg cacgctgcga gacgctccgt ccacccgcct
    10201 gagccggtgg acgcctccgc aacggccccc tatactgtgc tttcggtccc cggacggggg
    10261 ccggttctcg ctcgcccccc gagcgcacgg cgaccgtccg gcgtgcccgg agggcggagg
    10321 aggagcactg gtgaaggtac gtccgtcggt gaagaagatg tgcgagaagt gcaaggtgat
    10381 ccgccgtcgt ggacgggtga tggtcatctg cagcaaccct cgccacaagc agcgacaggg
    10441 ataggagcgg gatggcgcgc atcgcaggtg tcgaccttcc ccgcgacaag cgggtcgagg
    10501 tcgcgctgac ctacatctac gggatcggcc ggacgaaggc gtccgagacg gtccgccgga
    10561 cgggtctcga ccaggcgacc cgcgtccgtg acctctccga ggacgaggtc gtccggatcc
    10621 gcgactggat cgaccagaac tacaaggtcg agggtgacct ccggcgggac gtcgcccagg
    10681 acatcaagcg gaagatcgag atcagcgcct accaggggct gcggcaccgg cggagcctgc
    10741 cggtccacgg ccagcggacg cacacgaacg cgcggacccg caagggcccg aagaagaccg
    10801 tcggcgtgaa gaggaagaag tagatggcga agccgaagaa gaaggtccgc aagaaggaga
    10861 agaagaacgt cacgcacggt gccgcccaca tcaagtcgac gttcaacaac acgatcgtga
    10921 cgatcaccga ccccgacggg aacaccctcg cctgggcgag cgccgggaac gtcgggttca
    10981 aggggtcgcg caagtcgacg ccgttcgccg cgcagctggc agcggaggcc gccgctcgcc
    11041 gggcgcagga gcacggcctg cgcaaggtcg acgtcttcgt caagggtccg ggctcgggtc
    11101 gggagaccgc gatccgctcg ctcgccgccg cggggctcga gatcctcgcg atccaggacg
    11161 tcaccccggt cccgcacaac ggatgccgtc cgaagaagcg gaggcgcgtc tagatggctc
    11221 gctacaacga cgccgactgc aagctctgcc gccgcgagcg catgaagctc tacctcaagg
    11281 gcacgaagtg cgagaccccg aagtgcccga tcgagcgcag gccctacccg cccggccagc
    11341 acggccgcgc tcgcatcaag gagtccgagt acctgatgca gctccgtgag aagcagaagg
    11401 cgcgccggat ctacggcgtc atggagcggc agttccgcaa ctactacgag tcggccgccc
    11461 gtcagcgggg gatcaccggc gagaacctgc tgcgcctgct cgagacccgc ctcgacaacg
    11521 tcgtctaccg ggcggggttc gccacctcgc gcgaccaggc ccgccagctc gtgctgcacc
    11581 gtcacttcca ggtgaacggg aagatcgtga acatcccctc gtaccgggtg cgtcccggcg
    11641 acgtggtcga ggttcgcgag aagagccgcc ggctcatccc ggtccaggag gcggtggagc
    11701 tcgcggaggg gaagatcatc ccgccgtggc tggacgtgaa caagggccag ctcagcgtcc
    11761 gtgtcgcaga cctgccacag cgggtgatga tcgacacccc cgtgcaggaa cagctcatcg
    11821 tggagctcta ctcgaagtag ggaccgagcc ccaccggcgg gagccggcgg ggagtgggcc
    11881 gggcaggaac ggatgatccc cggccgaagg agggatacag cttgctgatc gtcgccagac
    11941 cacggatcca ggaagagacc gtcaacgacc ggcgctcgcg cttcatcatc gagccgctcg
    12001 agcccgggtt cggctacacc ctcgggaact cgctgcggcg gaccctgctc tcgtcgatcc
    12061 cgggcgcggc cgtcgcgtcg gtgaagatcg agggcgtgct gcacgagttc tccaccgtcc
    12121 cgaaggtgac ggaggacgtc accgacatca tcctgaacat caaggagatg gtgctccgcg
    12181 ccgacgtcga cgagccggcc acgatctacc tcaaggcgaa gggagccgcc gaggtgaccg
    12241 cgggcgacat cgcgcccccg gcaggcgtcg agatcctcaa tcccgaactc cacatcgcca
    12301 cgctcgccaa gggtgggtcg ctcgagatgg agatggtcgt cgagcgcggt gtcggctacc
    12361 ggtcggcgga gcagaacaag aagccgcgcg acccgatcgg cgtgatcccg atcgactcga
    12421 tgttctcgcc cgtgcgccgg gtctcgtact cggtcgaggc gacccgggtc gagcagatga
    12481 ccgaccgcga caagctgatt ctcgacgtcg agacgaacgg ggccatctcg ccgcgcgagg
    12541 ccgtcgcctc ggcggggacg acgctcgtgg agctctccgg gctcttctcc gagctctcgg
    12601 aggccgcggg tctctcgctc ggcccggccg agggcgaggc gcagccgagc ggcgagctcg
    12661 cgctgccgat cgaggagctc acgttctccg tccgctccta caactgcctg aagcgcgagg
    12721 gcgtgaacac cgtcgccgac ctcgtgcaga agagcgagca ggagctgatg gacatccgga
    12781 acttcgggca gaagtcgatc gacgaggtca aggccaagct cgaggagctg ggcctgtccc
    12841 tgcgggagga gtagcgtgcc gaagccgaag aagggaccgc ggctcggggc cgggccgacg
    12901 catcagaagc tcatgctcgg cggcctcgca tcgtcgctgt tccagcacga gcgcgtgcgc
    12961 acgaccgagg cgaaggcgaa ggcgctgcgt ccggtcgccg agcggctcat cacgttcgcc
    13021 aagcggggcg acgtcgccgc acgacgcgag gtcctgaagc tcgtgccgga ccgcgacgtc
    13081 gttcaccgcc tcttcgcgga catcgcgccg cgctacgccg agcgcccggg aggctacacg
    13141 cggatcctga agctcggccc ccgcaagggc gacggcgcgc cgatggccca gatcgagctg
    13201 gtcgaggaag cgaccgacga ctagggtcgt ccggctggtc ctcgcctacg acgggaccgg
    13261 tttccgtggc tgggcccggc agcccggaca gcggacggtg cagggagagc tcgagagggc
    13321 gctggagcgg gtcctcgggc gagtcccgcg gatcgcggtc gcgggccgga ccgacgccgg
    13381 ggtccacgcg gccgggcagg tcgtctcgtt cgcggcggac gcggatcccc agcggatcgg
    13441 gcgcgcggtg aacgggatgc tggcgccgga ggtggccgtc ctgcaggccg ggagggcgcc
    13501 tgcggggttc gacgcccggc ggagcgcgac cgggcgcgcg taccgctacc ggttacgccg
    13561 cgcggggacg ccggatccgt tcacggcgcg ctacgtctgg caccgcccgg gcgagtaccg
    13621 ggtcggacgg atgcggcggg cggcgcggct gctcgagggg gagcacgact tcacctcgtt
    13681 cagccggcgg gccccaggcg tgccgccggt ccgccgcctc cagcggctga gcgtggcggt
    13741 ggtgggagag gagctggtga tcggggcgac ggcggacggg ttcctgcacc agatggtgcg
    13801 gagcctcgtc ggaacgctgg tcgccgtggg cgaagggaag atggaggcgg aggcgatgac
    13861 ggaggtgctg gaggcccggt cgagggcggc tgcggggccg gtggcccccc cgcacggcct
    13921 gaccctcgtt cgggtacgat atggcgttct tgggacggag cgatgaagac ctactccccg
    13981 aaagcagcag acatcacgcg aggctggtac ctcgtcgacg ccgagggcgt cgtgctgggc
    14041 cgtctcgcgt ccgaggtggc gaagatcctg cgtggcaagc acaagccgac cttcgcgccc
    14101 cacgtcgacg gcggtgacca cgtcgtcgtg atcaacgccg cgaaggtgag tctctccggc
    14161 ggcaaggagt cgaagaagat ggcctaccgc cactcgcagt accccggcgg gctccgggcg
    14221 gtcccgtacg agcgcctgct cgcggagcgt cccgagcggg cggtcgagaa ggcgatcaag
    14281 ggcatgcttc cgaagaaccg cctcgggcgc cagatgggga agaagctgcg ggtctacgcc
    14341 gggcccgacc atcagcagca ggcccagcag ccggtgccgc tgaaggtggg cgacgtgccg
    14401 ccggcggtgc cgaggctgcc tggtgcgggg accgcggaga acgggaagga gtcttccgat
    14461 ggctgacacg aagaccgagc tggcccgcgc caccgggcgg cggaaggagg cggtcgcccg
    14521 cgtgcggatc gtcgagggca ccggggagtt ccacatcgac ggtcgcagcc tcgaggatta
    14581 cttcccgtcc cggacgcatc ggatgctggt gacggcgccc ctgcgcgagc tcggacgcga
    14641 gaagcaggtc gacgtcatcg cctccatcca cggcggcggc gcgagcggcc aagcgggggc
    14701 cctgcgtcac ggcatcgcgc gcgcgctcgt ggcctggacc gaggaggcta gggggagcct
    14761 gaagaaggag gggttcctcc gccgcgacgc gcgggagaag gagcgtcgca aggccggcct
    14821 caagaaggcg cgcaaggcgc cccagtacag caagcgctag ctccgccgtg gccctgttcg
    14881 ggaccgacgg catccgtggg atcgccaacc gcgacctgac ccccgagctg gcgttcgacc
    14941 tcggccgcgc agcggccgcc gtcctcggcg gggacgggcg ggctcgcttc gcgatcggcc
    15001 gggacacgcg cgggtcgggc gagatgctcg aggcggcgct cgcggccggc atctgctcga
    15061 tgggcggcga cgtcctgcgg ctcggcgtca cgcccacacc agcggtcgcc ttcctgacgg
    15121 tcgagctcgg ggcgggcggc ggcgtggtca tctcggcgtc gcacaacccc gcggagtaca
    15181 acggcatcaa gcttttcgcg cgagacggga cgaagctccc cgacgagctg gaggccgagg
    15241 tcgagcgcct gctgcaggac cggcccgtcg atcgaccgga ggggggcggc atcggccgga
    15301 tcgccgaggc tccggaggcc ggggaccgct acctcgcgca cgtgatcgac gcggcacggg
    15361 ggtccctgga ggggatgcgg gtcgtcgtcg actgcgcgaa cggggcggcg tcggagctcg
    15421 cgccgacggc actgcgccgg ctcggcgccg aggtcctgcc gatcaacgac cgccccgacg
    15481 gcaccaacat caacgccggc tgtggcgcga cgcacccgga ggttctcgcg gccgaggtcc
    15541 gccgcgtcgg tgccgatgcc ggggtcgcgc acgacggtga cgccgaccgg gcgatcttct
    15601 ccgacgcgga cgggacgatc gtcgacggcg atcacgtgct cgcggccgag gcgatcgcga
    15661 tgcgcgagga ggggagcctc gccgggaacg ccgtcgtcgc gaccgtcatg gccaacctcg
    15721 gcttccggcg cgcgatggag gccgcgggga tcggcgtcgt ggagacgaag gtcggcgacc
    15781 gctacgtcct cgaggagatg cgccggatcg gcgccagcct cgggggcgag cagtcggggc
    15841 acatcgtctt cctcgaccgc gccaccaccg gggacgggct cctgaccgcg gttcggttcc
    15901 tctccctcgc gcggcgccgc ggcgtcggcc tctcggagct cgcttccgtc gtcgagaagt
    15961 atccccagga gctgctcggc gtcgaggccc ccgacccggg cggtctggcc gccgacccgg
    16021 cggtcatcga ggccgtgcga gccgctgagg cctccctcgg ggggtccggg cgggtcctcg
    16081 tgcggccctc gggaacggag cccctcctgc ggatcatggt cgaggccgag gagctcgagc
    16141 aggcccgcaa gcacgccgag gcgatcgcgg acgcggcacg ctccgcggct ccctgagctg
    16201 ccggtccgtc ccggcacccc ctgctacgat gaacgtctca tgtgcggcat cgtgggctac
    16261 gtcgggcccg acgaagcgct ccccatcgtc ctcgaggggc ttcgtcgttt ggagtaccgc
    16321 ggatacgact ccgccggcgt cgccgtgctc gacggcggcc tcgacgtggt gaagcgcgcg
    16381 ggcaagctcg aggtcctcga caaggagctg gcccagcagg ggatgcccga aggccaggtc
    16441 gggatgggac acacgcggtg ggcgacgcac ggccagccga ccgaccgcaa cgcccacccc
    16501 cacaccgact gccgggaccg ggtcgcggtc atccacaacg ggatcatcga gaacttccat
    16561 gtgctgaagg accgtctcga ggccgacggc cacgagttcc gctccgacac cgacaccgag
    16621 gtcgtggcgc acctgatcga ggagcgcctc gacggcgatc tcgccgccgc ggtgcgctcg
    16681 gcggtcgcgg agctcgaggg ggcgttcgcg ctggtggcct tgagcaccga ggagcctgac
    16741 gtggtcgtcg gcgtccgggt ctcctccccg atggtcgtcg gcttgggcga tggcgagaac
    16801 ctcctcgcct cggacgtgcc ggcgctcatc gaccggactc gccgcgtcgt cccgctctcc
    16861 gacggccagg tcgtggagct ccgggccggc tccgtgcgga tcaccgacct cgacggccgc
    16921 gaggtctcgg tcgagccgat ggagatcgac tgggatctgc gccgcgccga gaaggagggc
    16981 ttcgaggact tcatgctcaa ggagatccac gagcagccga cggcgatccg cgacacgatg
    17041 cgcgggcgca cgagggaggg tcgcctcgtc ctcgacgagc tgcggatgag cgacgaggac
    17101 atccgcgccg tcgacaaggt cttcgtcgtc gcctgcggga ccgcgtacca cgccggactc
    17161 gtcgcgaagt acgcgatcga gcactgggcc cggctgccgg tcgagatcga gatcgcgagc
    17221 gagttccgct accgggaccc ggtcctggac cggaacacgc tcacgctcgc ggtgtcgcag
    17281 tcgggggaga cgatcgacac cctcgaggcg ctgcgccatg cgaagcgcca gcactcgtgg
    17341 gtcctcgcca tcacgaacac ggtcggctcc tcggtcgcac gcgagtcgga cgcggtcctg
    17401 tacacgcacg cgggtccgga gatcggggtc gcggcgacga agaccttcgc cacccagatg
    17461 gtggccctcc acctcgtcgc cctctacctc gcgcaggtcc gcggcgtgat gtacccgcgc
    17521 gagatcgccg aggtcctcga ccgcatgaag cgcctccccg accagatcgc cacgaccgtc
    17581 aggttggacg agcagatgcg gggcctcgcg cagcgctacc aggatgcccg cgacgtcctg
    17641 ttcatcggac ggcacaccgg ctacccatcg gcgctcgagg gcgcgctgaa gctcaaggag
    17701 atctcctaca tccacgcgga ggggtacccc gcgggggagc tgaagcacgg gccgatcgcg
    17761 ctcgtggagg agggcgtgcc ggtggtggcg gtggcgacgg agtgccacgt ctacccgaag
    17821 atgctctcca acatccagga ggtgagggct cggggcgccg aggtcgtcgc gatcgcgacc
    17881 gagggcgacc gcgacatcga gaagcacgcc acgcacgtcg tgaccgtccc ccggacccac
    17941 gagctcctgt cgccggtggt cgtcgcggtg cccctccagc tcctcgcgta ccacgtggcg
    18001 aagctccgag gctgcgacgt cgaccaaccg cggaacctcg cgaagtccgt cacggtggag
    18061 tagcccgccg tggagatcct cgggatcggc gtcgacgtcg tggacgtcgg ccggatccgg
    18121 cggatgctcg agcggcaccg gcggttccgc gagcgcgtgt tcacccccga tgaggtcgcc
    18181 tactgcgaga cgaaggcgaa ccgtgccgaa cgctacgccg ctcggtgggc ggcacgcgaa
    18241 gccacgatca aggcgctggg cggggtccgc ggcctccggt accacgacat cagcgtcggg
    18301 cgccatcggt cgggcgctcc ccgcatcgtt ctgtcgggaa gcgctcgcgc gcgcgcggct
    18361 gaccgcgggg tgcgcgacgt gctggtttcc ttctcgcacg agcgcgacac cacggtcgct
    18421 ttctgcatcg ccgtcgggag agccccgtga gaccggtgct gggggcggcg caggcgaaag
    18481 agctcgatgc cgcgtcgcag gagcggggcg tgccggccga cgtcctgatg gagcgggcgg
    18541 gccacgccct cgcgcgcgag gccgtgagca tcgccggggg cgccgccggg cgccgcgtcg
    18601 tggtcgtcgc ggggaccggc aacaacgggg gagacggcct cgttgccgca cgccgtctcc
    18661 ggggtcgtgg cgccggcgtg tcggtgctcc tggtcggcga ccgctcccgc ctgagccccc
    18721 tcgcggaggc gaacctcgga cgcatccacg acgtccccgt ttggcccttc tcctcatcga
    18781 cgatcgagcg cgcgctcgac cgggccgacg tggcgatcga cgcgatcttc ggggtcggct
    18841 tccgcgggag cgccgaaggc gacgcggccg ccgcgatcgc ggccctgaac gcgtcgccgg
    18901 tgccggtggt ggcggccgac gtcccatccg gggtcgacgc ggacctcggc gcggtccgcg
    18961 ggccggcggt cctcgcgacg cgaaccgtta cgttcggcgc gccgaagacg gggatcatcc
    19021 tgttcccagg ggccgcgcac gccggggagg tcgccgtcgc cgacatcggc ttccccccgg
    19081 acctgatccg ctcggacgtg ctcttggtcg acgagg
//
LOCUS       DATLGG010000124         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_135084, whole genome shotgun sequence.
ACCESSION   DATLGG010000124 DATLGG010000000
VERSION     DATLGG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135084"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VM638_03615"
     CDS             <1..239
                     /locus_tag="VM638_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVM11550.1"
                     /translation="LFFLFAGFNVFVGILNLLPLPPLDGGHLAVLGIEKATGRRIDPR
                     KLLPITAFVVAYLMLFFVSITATDILRPLPNPFR"
     gene            253..1347
                     /gene="ispG"
                     /locus_tag="VM638_03620"
                     /gene_synonym="gcpE"
     CDS             253..1347
                     /gene="ispG"
                     /locus_tag="VM638_03620"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVM11551.1"
                     /translation="MGIERRRSRQVKVGWVTVGGDAPIRLQSMTTTKTADVNATLQQM
                     AALVATGCEIVRVAVPHKEDAEALRQIAEHATIPVVADIHFQWRYAIMALEAGIHGLR
                     INPGNIKYQDKVRLIAREAKARGVPIRIGVNAGSLEPDLLARYGSPTPEALVESALNE
                     ARILEDEDFFDIKISVKHSNPLRMIEAYRMLAEKCDYPLHLGVTEAGPMPSGGVKSAV
                     GIGTLLAEGIGDTIRVSLTDDPVEEVKVGAWILQSLGLRKRGLDLVACPSCGRAEVDV
                     LGLTKQVNEALEAEGIKAPLRVAVMGCVVNGPGEAREADLGIAAGKGYGQLIVRGEVQ
                     ERRIPEAKLVEAVVEEARRLVADSERATEV"
     gene            1371..>2993
                     /locus_tag="VM638_03625"
     CDS             1371..>2993
                     /locus_tag="VM638_03625"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HVM11552.1"
                     /translation="MRTSELFARTLREAPADEEAPSARLLLRAAFVRKLMSGVYTMLP
                     LGLRVLRRIEAIVREEMNAAGAQEVLMPALQPSEPWKQTGRWQAYTAEELMFTLTDRA
                     GREIGLGPTHEECVTPLVDHDYSSYRDLPVNLYQVQWKYRDEARPRSGLVRGREFLMK
                     DAYSFDRDVEGLKVSYRKMVEAYQRIFDRCGLTYRVVEADPGLIGGDVNHEFMAPAGI
                     GEDLFVHCDTCDYAANVEAASASVPEAGDPEGDLAAVEVVATPGAATIEAVAEQLGVE
                     AGRVLKCLVYRVGHKMVAVLVPGDREVSEGKLRRLFAPDEVEMLTDFEAAGLVRGFVG
                     PQGLEGIEIVADPRVRLGANWVAGANEPDAHATGVNPGRDFRVDRWEEVATVHEGDRC
                     PRCETGTLRMGRSIEVGHTFQLGTKYSDPLKARFLDEDGVEKPFVMGCYGIGVSRIVA
                     SVVEQHNDDAGIVWPKAVAPFQVVVIPTNMDQPEVVEAAERLYGELGAGGIEAVIDDR
                     DARAGVKFADADLIGYPVQVVVGKKGLERGVIE"
BASE COUNT          456 a          983 c         1117 g          437 t
ORIGIN      
        1 tgctgttctt cctcttcgcc ggcttcaacg tcttcgtggg catcctcaac ctgctgccgc
       61 tgcccccgct cgacggcggc catctcgcgg tgctcgggat cgagaaggcg acggggcggc
      121 ggatcgaccc gaggaagctg ctgcccatca ccgccttcgt cgtcgcgtac ctgatgctgt
      181 tcttcgtctc gatcaccgcg accgacatcc tgcgtccgct gccgaacccg ttccggtagc
      241 tagactgaag ccgtgggcat cgagcgccgc agatcgcggc aggtgaaggt cgggtgggtg
      301 accgtcggcg gggacgcccc gatccggctc cagtcgatga cgacgacgaa gaccgccgac
      361 gtcaacgcga cgctccagca gatggcggcc ctcgtcgcga ccgggtgcga gatcgtgcgc
      421 gtcgcggtcc cccacaagga ggacgcggag gcgctccggc agatcgccga gcacgccacg
      481 atcccggtcg tcgcggacat ccacttccag tggcgctacg cgatcatggc gctcgaggcc
      541 ggcatccacg gcctgcggat caaccccggc aacatcaagt accaggacaa ggtgcggctg
      601 atcgcgcgcg aggcgaaggc gcggggcgtc ccgatccgga tcggcgtcaa cgccggcagc
      661 ctcgagcccg acctgctcgc ccgctacggc tcgccgacgc cggaggcgct cgtcgagtcc
      721 gccctgaacg aagcgcggat cctcgaggat gaggacttct tcgacatcaa gatctcggtg
      781 aagcactcga acccgctgcg gatgatcgag gcctaccgga tgctcgccga gaagtgcgac
      841 tacccgctgc acctcggggt gaccgaggcg ggcccgatgc cgtccggcgg cgtgaagtcc
      901 gcggtcggga tcgggacgct cctcgcggag gggatcggcg acacgatccg ggtctcactg
      961 acggacgatc ccgtggagga ggtgaaggtc ggggcctgga tcctccagtc gctcggcctt
     1021 cgcaagcgcg gcctcgacct cgtcgcctgc ccctcgtgcg ggcgcgcgga ggtcgacgtc
     1081 cttgggctca cgaagcaggt caacgaggcg ctcgaggccg aggggatcaa ggcgcccctg
     1141 cgcgtcgccg tgatgggctg cgtcgtcaac ggcccgggcg aggcccgcga ggcggacctc
     1201 gggatcgccg ccggcaaggg ctacggccag ctgatcgtcc gcggcgaggt ccaggagcgc
     1261 cgcatccccg aggcgaagct cgtcgaggct gtcgtcgagg aagcccggcg gctggtggcg
     1321 gactcggagc gcgcgaccga ggtttaagcc gctcgcgtat cctgagcggc atgcgcacga
     1381 gcgagctgtt cgcccgtacc ctgcgggagg cccccgccga tgaggaggcc ccgtcggcgc
     1441 ggctgctgct gcgggccgcc ttcgtccgga agctgatgtc cggcgtctac acgatgctcc
     1501 cgctcggcct gcgggtcctg cgacggatcg aagcgatcgt ccgcgaggag atgaacgccg
     1561 cgggcgcgca ggaggtcctg atgccggcac tccagccgtc ggagccgtgg aagcagacgg
     1621 gccgctggca ggcgtacacg gcggaggagc tgatgttcac gctcaccgac cgcgcgggcc
     1681 gcgagatcgg gctcggcccg acgcacgagg agtgcgtcac acctctcgtc gatcacgact
     1741 actcctcgta ccgcgacctc ccggtgaacc tctaccaggt ccagtggaag taccgcgacg
     1801 aggcgcgccc gcgctccggc ctcgtgcgcg gccgcgagtt cctgatgaag gacgcgtact
     1861 cgttcgaccg cgacgtcgag gggctgaagg tgtcgtaccg gaagatggtc gaggcgtacc
     1921 agcggatctt cgaccggtgc gggctcacgt accgggtcgt cgaggcggac cccgggctga
     1981 tcggcggtga cgtgaaccac gagttcatgg cgcccgcggg gatcggggag gacctcttcg
     2041 tgcactgcga cacgtgcgac tacgcggcga acgtggaggc ggcctcggca tcggtcccgg
     2101 aggcgggtga cccggagggg gacctcgccg cggtcgaggt ggtcgccacc cccggtgcgg
     2161 cgacgatcga ggcggtggcc gagcagctcg gagtggaggc gggacgggtc ctgaagtgcc
     2221 tcgtgtaccg cgtcggtcac aagatggtcg cggtgctcgt gcccggggac cgcgaggtga
     2281 gcgaaggcaa gctccggcgt ctcttcgcgc cggacgaggt tgagatgctc acggatttcg
     2341 aggcggcggg gctggtccgc ggcttcgtgg gcccgcaagg gctggagggg atcgagatcg
     2401 tggccgaccc gcgggtgcgc ctcggggcga actgggtcgc gggagccaac gaacccgatg
     2461 cccatgcgac cggtgtgaat cccgggcgcg acttccgcgt cgatcggtgg gaggaggtcg
     2521 cgacggtcca cgagggcgac cgctgtcccc gctgcgagac gggaacgctg cggatggggc
     2581 gctcgatcga ggtcgggcac acgttccagc tcggcacgaa gtactccgac ccgctcaagg
     2641 cgcggttcct cgacgaggac ggcgtcgaga agccgttcgt gatgggctgt tacgggatcg
     2701 gcgtgtcgag gatcgtcgcc tcggtggtcg agcagcacaa cgacgacgcg gggatcgtct
     2761 ggccgaaggc cgtcgcgccg ttccaggtcg tcgtgatccc gacgaacatg gaccagcccg
     2821 aggtcgtcga ggcggcggag cggctctacg gggaactcgg cgcgggcggg atcgaggccg
     2881 tgatcgacga ccgggatgcc cgcgccggcg tgaagttcgc cgacgccgac ctgatcggct
     2941 accccgtcca ggtggtggtc gggaagaagg gtctcgagcg cggtgtcatc gag
//
LOCUS       DATLGG010000125         5338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_135117, whole genome shotgun sequence.
ACCESSION   DATLGG010000125 DATLGG010000000
VERSION     DATLGG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135117"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VM638_03630"
     CDS             complement(<1..868)
                     /locus_tag="VM638_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11553.1"
                     /translation="MSDRGSVRSYAAAGITLVVLLGVVRGFDRADAEQARKMQERAAL
                     EHLVLVQSRLEQGLHRRLALAEGLRAFVGTGHLSRSSFRAFARGLLARDEGIDYVALA
                     RNGVLRHAYPPAAVSGIAGLASARSGPSDRLAPRTPALTIAGPIRLGTRTRLVARLPV
                     STGHGPHRQTWGIAIVAMNLDALLEDAGVRGGPFQLALRVAPEEGRRRPAFFGSDLVF
                     RSDPQVIEVGLPGRTWELAAAPVGGWTSGSPMAWAWRGGGVLLALLGAILMWFLVDAP
                     RRLRVAVSTATRS"
     gene            1127..2044
                     /locus_tag="VM638_03635"
     CDS             1127..2044
                     /locus_tag="VM638_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11554.1"
                     /translation="MKKISTRAAALAALLVLTAGSASAQPQLAITTPKDGTTVSRSES
                     SSILVSGAVQFEVPTASTRRFYLRRNGCGLNQDNTIYLSVINATNGGNGCGYTGGWNL
                     VGTVDESGFSDQYQAVDGIPFTLDASRPVAIRVRLSNYQGVSNNLVSLGAGFATLTAT
                     LSADGAAGTQTLGSQAVTKTILPTQITYDYDFSFTLPPESEKKDYTGLSLDLSMTGPG
                     VNHGWVMLNGGVSYVDLPIYTASFSRRVEIGVDSGSFSATGVTLNLEQGTWSRTMSTP
                     SVGSRVIKARAVQGSLISAVDQVTIQVVS"
     gene            2242..3120
                     /locus_tag="VM638_03640"
     CDS             2242..3120
                     /locus_tag="VM638_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11555.1"
                     /translation="MTIKTRRVVLALAGLMALTGAAAAQPTVAITAPADGSTISRSAT
                     PQIAVSGQSAFEEPVPTGRNLYLRWDGTDDSCGATYLSDNNGPDEGNGCAFVAGIASV
                     TPDEDDFTVSYSSGAEVATPITIDATRRITGTMGYVHAAPDAPIFDIEVLFDDQVVGR
                     QRYNPGPQVGLFARRQFNLDIPIPQSWHQKDVSSVTLNWHFQQSLSVASTWVEHENPV
                     SFMRLPTYSASFSRKVEVQLNNGSWQPATLSQDLTTWTRAVNAPAANGPAVIRARAVQ
                     GGSTSGVSQISVTIEP"
     gene            3309..4208
                     /locus_tag="VM638_03645"
     CDS             3309..4208
                     /locus_tag="VM638_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11556.1"
                     /translation="MRKLPIQLAVAALAVALVAPAATAEPEIAITTPADGSTISRSTT
                     SSILVSGNSLFEEPQQSNARFFLRRAGCGTANDNQRLSRTSGTDNSGCAYTASITPLN
                     ELDGGSLADSYPAADGVPLTLDATKPISGVIQVSSYQPGVNAGVGMTRIDITLRGSER
                     AGGETQIGTTSVSYTVLPGQAPVRSSWSFQPVASLDKKDFTSLELSVVVRGHNVMHGF
                     TNYGGSSYLDIPSWTKSFDRRVEIAVDNGAFSSTGVTYNAERTTWSRNLTTPSAGPHV
                     IKARAVQGSVVSAVDQVAIQVVS"
     gene            4378..5271
                     /locus_tag="VM638_03650"
     CDS             4378..5271
                     /locus_tag="VM638_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11557.1"
                     /translation="MRTMKPWVLATTLAVALLAPTSGHAVDEISITTPAEGATISRSA
                     TPAMTLSGGVAFEEPVPTTRTLYLRWNGTPGSTECSMSDAYLADVSGVDPGNGCSGFP
                     GAALTALPGEDDYNNRFPSGEHVKTPITIDASRNITGTITVRGVLNQIPNLAIIDVVV
                     ELDEVALRQRFEDAQPTTTSRRDLSLNIDIPAELDRKDVSSVTLTVYFQQYLGAGWTE
                     VENPASSFRLPAYSASFNRRVELNFDNPTTRFTATLSQDATSWTASRSTPPIGSRTLT
                     ARAIQGGTVLSTATRGFTVTG"
BASE COUNT          902 a         1929 c         1797 g          710 t
ORIGIN      
        1 ggctccgcgt ggccgtgctc accgccacgc ggagccgtcg aggagcgtcg accaggaacc
       61 acatgaggat cgcaccgagg agcgcgagca ggacgccccc accccgccag gcccacgcca
      121 tcggggagcc gctcgtccag cccccgacgg gggctgcggc gagctcccag gttcgcccgg
      181 ggaggccaac ctcgatcacc tgcgggtccg agcggaagac gaggtcggag ccgaagaagg
      241 ccggccgccg ccgcccctcc tccggcgcga cccgcaaggc gagctggaac ggtccccctc
      301 gcaccccggc gtcctccaag agggcatcga ggttcatcgc gacgatcgcg atcccccacg
      361 tctgccgatg cggcccgtgt cccgtggaga ccggaagccg agcgacgagg cgcgtgcggg
      421 tccccagccg gatcggcccg gcgatcgtca gggcgggtgt gcggggcgcg agacggtccg
      481 agggaccgga cctcgcggag gcgagacccg cgatgccgct gacggctgcc ggcggatacg
      541 cgtggcggag gacgccgttg cgagccagcg cgacgtagtc gatgccctca tcgcgtgcga
      601 gcagcccgcg cgcgaacgca cggaaggagc ttcgggagag gtggccggta ccgacgaacg
      661 cgcgcaggcc ctcggcgaga gcgagccggc ggtgcagccc ctgctcgaga cgggactgga
      721 cgaggacgag atgctcgagt gccgcccgtt cctgcatctt cctcgcctgc tcggcgtcgg
      781 cgcggtcgaa cccgcggacg actcccagga gcaccacgag ggtgatcccc gccgctgcgt
      841 acgaccggac gcttccccga tccgacaccg tgctcccgtt atcggccttc caggtcgccg
      901 gcgacatccc ccgatggggt caatctgcca gggagccgct gacccgtcgt gactagtttc
      961 gccgggacga acccctgtct tcccgacact tcgccgaggg gcggcggagc ctccgccccg
     1021 ggtggcggct cacttgtgca cccgcacgaa gggctcggcc ggagcgaagc ggaacacgtg
     1081 gtcgaacacg gacaggtccc tcggaggggg ccggaaggag ggaacgatga agaagatctc
     1141 cacgcgggcc gcggccctcg ctgcgctgct cgtgctgacc gcgggaagcg cgtccgccca
     1201 gccgcagctg gcgatcacga ccccgaagga cgggacgacg gtctcgcggt cggagagctc
     1261 ctcgatcctg gtatcgggcg cggtgcagtt cgaggtgccg accgcgtcca cgcggcgctt
     1321 ctacctgcgg cggaacgggt gcggcctgaa ccaggacaac acgatctacc tgagcgtcat
     1381 caacgccacg aacggcggca acggctgcgg gtacacgggc ggctggaact tggtgggcac
     1441 ggtggatgag agcgggttct cggaccagta ccaggcggtc gatgggatcc ccttcacgct
     1501 cgacgcctcg cggccggtcg cgatccgcgt ccgactgtcg aactaccagg gcgtgagcaa
     1561 caacctcgtc agcctcggcg ccggcttcgc gacgctcacc gccacgcttt cggctgacgg
     1621 ggcagcgggc acgcagacgc tcggatcgca agccgtgacg aagacgatcc tgccgacgca
     1681 gatcacctac gactacgact tcagcttcac gctgccaccg gagtcggaga agaaggacta
     1741 caccgggctc tccctggacc tgtcgatgac cggacccggg gtgaaccacg ggtgggtgat
     1801 gctgaacggt ggcgtctcct acgtcgacct gccgatctac acggcgagct tcagccgtcg
     1861 cgtcgagatc ggcgtcgact cggggagctt cagcgcaacc ggcgtcaccc tgaacctcga
     1921 gcaggggacc tggagccgca cgatgagcac gccctcggtc gggtcccgcg tcatcaaggc
     1981 gcgagcggtc cagggcagtc tcatctcggc cgtcgaccag gtgacgatcc aggtcgtgag
     2041 ctagacctcc tgccgttcct ccacgaaggg cgcccctgac gggggcgccc ttcgctctat
     2101 cggccgcccg ctgtggggac ggcgggaaca ccgtcaggat tgtgcacttg cacaaaggat
     2161 tcgagcgcct cgacgcgcaa caacctgagc agggcaccac gtcgaggccg ccatccgctt
     2221 gggcggtcgg aaggaggaac gatgaccatc aagacccgcc gggtcgtcct ggcgctcgca
     2281 ggcctgatgg cgctcaccgg cgctgcggcc gcccaaccca cggtcgccat caccgcgccg
     2341 gccgacggct ccacgatcag ccgctcggcc acgccgcaga tcgcggtgag cgggcagtcc
     2401 gccttcgaag agccggttcc caccggccgg aacctgtacc tccgctggga cggcaccgac
     2461 gactcctgcg gagccaccta cctctccgac aacaacggac ccgacgaggg caacggctgc
     2521 gcgttcgtgg ctggtatcgc gtcggtcacg cccgacgagg atgacttcac cgtcagctac
     2581 tcatccggag cggaggtggc cacgccgatc acgatcgacg ccaccaggag gatcaccggg
     2641 acgatgggct acgtgcacgc cgccccggac gcgccgatct tcgacatcga ggtgctcttc
     2701 gacgaccagg tcgtcgggcg gcagcgctac aacccggggc cgcaggtcgg cctgttcgcg
     2761 cggcggcagt tcaacctcga catcccgatc ccgcagtcct ggcaccagaa ggacgtgagc
     2821 tcggtgacgc tgaactggca cttccagcag tcgctcagcg tcgcctccac ctgggtcgag
     2881 cacgagaacc cggtttcgtt catgcggctc ccgacctact cggccagctt cagccggaag
     2941 gtcgaggttc agctgaacaa cggcagctgg cagccggcga cgctctcgca ggacctgacg
     3001 acgtggaccc gcgcggtcaa cgcccccgcg gcgaacggtc cggcggtgat ccgcgcacgc
     3061 gcggtccagg ggggctcgac gtccggggtc agccagatca gcgtgaccat cgagccgtag
     3121 cgaaccgaac gactgcagaa gggggcgccg ggtccggcgc ccccttcgtc gttgtctggg
     3181 atcgccagac ccgtcacggc gcggcgggtg ggccgccgat tttgtgcggc tgcacaaagg
     3241 gtttcctcgc aggcaaccgg aatacaaggt cagaacgctc gtcgatgcag cacgaaggag
     3301 gaaggtccat gcgcaagctt ccgatccagc tcgcggtcgc ggctctcgcc gtggcactcg
     3361 tggcgcccgc ggctaccgcc gagccggaga tcgccatcac gaccccggcc gatgggtcga
     3421 ccatctcccg gtcgaccacc tcgtcgatcc tcgtgagcgg gaactcgctc ttcgaggagc
     3481 cccagcagtc gaacgcccga ttcttcctgc gccgggcggg ctgcgggacc gccaacgaca
     3541 accagcggct cagccggacc tccggcaccg acaactccgg ttgcgcgtac accgccagca
     3601 tcaccccgct gaacgagctc gacggcggga gcctggcgga cagctacccc gccgccgacg
     3661 gcgtcccgct cacgctcgac gcgacgaagc ccatctccgg ggtcatccag gtttcgagct
     3721 accagcccgg cgtgaacgcc ggcgtgggga tgacgcggat cgacatcacc ctgcgcggca
     3781 gcgagcgggc cggcggcgag acgcagatcg gcacgacgag cgtctcgtac acggtcctgc
     3841 ccggccaggc gcccgtccgc agcagctggt cgttccagcc ggtcgcgagc ctcgacaaga
     3901 aggacttcac ctcgctcgag ctgtcggtcg tcgtccgcgg gcacaacgtc atgcacggct
     3961 tcaccaacta cggcgggagc tcctacctcg acatcccgtc gtggacgaag agcttcgacc
     4021 gtcgcgtgga gatcgcggtg gacaacgggg ccttcagctc gacgggcgtg acctacaacg
     4081 ccgagcggac gacgtggtcg cgcaacctga cgaccccgag cgccgggccc cacgtcatca
     4141 aggctcgcgc cgtccagggc agcgtcgtct cggccgtgga tcaggtagcg atccaggtcg
     4201 tcagctagaa caacatccgg gctcatcgaa ggggcgcctc cgggcgcccc ttcgcctatc
     4261 ccgatctggc acggggtggc ggtcgttccg ccggccctgc gccaggaacg gcatcccctc
     4321 ccgacgaaag gacccacgac cggccgccgc cccggcggca ggagggagga cgatgtcatg
     4381 aggacgatga agccctgggt gctggcgacc acgctcgcgg tggcgctcct cgctccgacg
     4441 agcgggcacg ccgtggacga gatctcgatc acgacgccgg cggagggcgc cacgatctca
     4501 cgcagcgcga cgccggcgat gaccctgtcc ggcggcgtgg ccttcgaaga gcccgtcccg
     4561 acgacgcgga cgctgtacct ccgctggaac gggacccccg gctcgacgga atgcagcatg
     4621 agcgacgcct acctcgcgga cgtgagcggc gtcgacccgg gcaacgggtg ctccggcttc
     4681 cccggcgccg ccctcacggc gctgccggga gaggacgact acaacaaccg cttcccctcc
     4741 ggcgagcacg tcaagacgcc catcacgatc gacgccagcc ggaacatcac cggaacgatc
     4801 acggtccggg gggtcctcaa ccagatcccg aacctcgcga tcatcgacgt cgtcgtcgag
     4861 ctcgacgagg tcgccctgcg ccagcggttc gaggacgcgc agccgacgac gacgagccgt
     4921 cgtgacctga gcctcaacat cgacatcccc gctgagctgg accgcaagga cgtctcgtcg
     4981 gtgacgctga ccgtctactt ccagcagtac ctcggggccg gctggaccga ggtggagaat
     5041 ccggcctcct ccttccgcct cccggcctac tccgccagct tcaaccgacg ggtcgagctg
     5101 aacttcgaca acccgacgac ccggttcacc gccacgctct cgcaggacgc gacgagctgg
     5161 accgcgagcc ggtcgacgcc gccgatcggg agccggaccc tcaccgcccg cgcgatccag
     5221 ggcgggaccg tgctcagcac cgcgacgagg ggcttcacgg tcaccggctg agccggcacc
     5281 acttcggata agaggggcgc ctccgggcgc tcctctcatc tcgcgaacga tgccacgg
//
LOCUS       DATLGG010000126         4571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_100226, whole genome shotgun sequence.
ACCESSION   DATLGG010000126 DATLGG010000000
VERSION     DATLGG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100226"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VM638_03655"
     CDS             <1..296
                     /locus_tag="VM638_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVM11558.1"
                     /translation="TGPRGAWARRHEVQFLDVREPAEVREGAIEGAVHVPLQRLLTTH
                     PDLDPERPVVAYCSAGYRSEVARQMLLARGFDAYNLEGGLDAWVRAGLPLRTP"
     gene            complement(298..2298)
                     /locus_tag="VM638_03660"
     CDS             complement(298..2298)
                     /locus_tag="VM638_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVM11559.1"
                     /translation="MTSQNRLAAATSPYLLQHASNPVDWYEWGEEALARAKREEKPIL
                     LSVGYAACHWCHVMERESFEDEATARLMNEHFVCVKVDREERPDIDAIYMDAVQAMTG
                     HGGWPMTVFLTPDLQPFYAGTYFPPEDRHGMPGFRSVLAAVARAWEEQREQVVTQGQR
                     VSEAIARSLPVADEPLEAGILDEAARSLERSFDRTWGGFGGAPKFPQPMNLGFLLRMH
                     RRGFPGAERLVTHTLDRMAAGGIHDHVGGGFHRYSTDERWLVPHFEKMLCDNALLARA
                     YLHAYQAFPERQHYREVCVRTLEYLLRDLHHPDGGFFSSQDADSEGEEGRFYVWPWEE
                     IVAVAGEEAAGALGASPGGNWEGTNVLTVDLTTEAADVARPALPALFEAREPRVKPAT
                     DDKVLAAWNGLAIQALAEAGRVLDEPRYIEAAVAAGAFVLEHLRRDDGRLLRAWRDGR
                     TSGLAYSDDHACMAAACLTLYETTFDLRWFDETRALADDLVRLFLDEEGGGFYQTGSD
                     AEALLMRPKELFDNAVPSGNSVAAEVLLRLALVTGEEGYERAATSALRAVGGVLLRAP
                     SMFGHALSALDLTLSRATEVAIVGDPDEAATRTLLDVVRKPYRPNLVVAAAREDDADA
                     TKTIPLLADRTARDGRATAYVCERFVCQQPVTSAEDLAAQLS"
     gene            2356..2610
                     /locus_tag="VM638_03665"
     CDS             2356..2610
                     /locus_tag="VM638_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11560.1"
                     /translation="MTDEGDDMDEQRFHDAILALEQATREEKAPEAWRAIGETAIEDF
                     WRAWPEIRAWGQWLWQLVDAERAEQAEPDHRADVDTGGPG"
     gene            complement(2657..3643)
                     /locus_tag="VM638_03670"
     CDS             complement(2657..3643)
                     /locus_tag="VM638_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11561.1"
                     /translation="MRRRFTLVTAIALLGSLSLVGQASANSVTLSPEFTLSGNNVTVS
                     GTASFPAITTPQSVGGTKTAFQGANGTPVAQAAGIDLAQNGGQIVPINGGLRFIWNVT
                     NMVDPQVGVPPDVVRYTWAFAIEKPDGTQTTYQLQAKRTNFTSLTWAEDPIGHLQNHY
                     NNRPWFQLRGACVDQYPGDGIATPVAGCAHLAFLPGTFDYANRQVRMDLMFRQRDTKG
                     RIVADEFAPGATLNAFNSASMSISGCFQAVVSSTATCNYINAWNPYYIGGRVQLGVGP
                     ATADPASVTYGAPVTLNNGAFTGTLTTSATNNTVFARACNGATCGYTSLRVR"
     gene            complement(3839..>4571)
                     /locus_tag="VM638_03675"
     CDS             complement(3839..>4571)
                     /locus_tag="VM638_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11562.1"
                     /translation="TISPIQDGLRFTWHLSSMPEQTLLEGIRYTWSFKIGETNYQLQA
                     KRVNLASTTTVEDPVGHVNRATSQQDFFQLRGACEPQYQGLPLAGCYHLTFLNGAFDP
                     ANKQVRVDLPFRARDRLGRIVAEDFVPGVVLESSLQAGASISAAFQAVVTYNDYPVTQ
                     LGRGFMSTYINEWSPYYIGGRVQLGVGSHLASPADVTYGSPVTLSAGRFTATISGLSS
                     TNNTVFVRACNGVTCSYASKRLIGG"
BASE COUNT          650 a         1569 c         1655 g          697 t
ORIGIN      
        1 gaactggacc tcgtggcgcc tgggcgcgtc gccacgaggt ccagttcctc gacgtgcggg
       61 agccggcgga ggtccgcgag ggcgcgatcg aaggggccgt gcacgttccg ctccagcgcc
      121 tgctgacgac ccatccggat ctcgatccgg agcggccggt cgtcgcctac tgcagcgcgg
      181 ggtaccgcag tgaggtcgcg cgccagatgc tgctcgcgcg cggcttcgac gcgtacaacc
      241 tcgagggtgg gctcgacgcc tgggtccgcg cgggtctgcc gctccggacg ccctgactca
      301 ggagagctgc gcggcgaggt cctcggcgct ggtcaccggc tgctggcaga cgaagcgctc
      361 gcagacgtag gccgtggctc gcccgtcgcg cgcggtgcgg tcggcgagca gggggatcgt
      421 cttggtcgcg tcggcgtcgt cctcccgcgc ggcggcgacg acgaggttgg gccgatacgg
      481 cttccggacg acatcgagga gcgtgcgcgt tgcggcctcg tccgggtcgc ccacgatcgc
      541 cacctccgtg gcgcgtgaca gcgtgaggtc gagcgcggac agcgcgtggc cgaacatcga
      601 cggcgcgcgc agcagcacgc ccccgacggc gcgaagcgcc gacgtcgccg cgcgctcgta
      661 gccctcctca ccggtcacga gcgcgagccg cagcagcacc tctgccgcca cggagttccc
      721 ggagggaacg gcgttgtcga acagctcctt ggggcgcatc agcagcgcct cggcatcgga
      781 gccggtctgg tagaagccgc cgccctcctc gtcgaggaag aggcgcacga ggtcgtcggc
      841 gagcgcccgc gtctcgtcga accaccgcag gtcgaaggtc gtctcgtaga gggtgaggca
      901 ggcggccgcc atgcacgcgt ggtcgtcgga gtaggcgagg ccgctcgtcc tgccgtcccg
      961 ccacgcgcgg agcagtcgcc cgtcgtcgcg ccgcaggtgc tcgaggacga acgcccccgc
     1021 cgcgaccgcc gcctcgatgt atcgaggctc gtcgaggacc ctgcccgcct ccgccagcgc
     1081 ttggatcgcg aggccgttcc acgccgcgag caccttgtcg tcggtggccg gcttcacccg
     1141 aggctcccgc gcctcgaaca gcgctgggag cgccgggcga gcgacgtcgg ctgcctccgt
     1201 cgtcaggtcg accgtgagca cgttcgtgcc ctcccagttc ccccccgggg acgcgccgag
     1261 cgcccccgcg gcctcctcgc cggcgacggc gacgatctcc tcccagggcc acacgtagaa
     1321 gcggccctcc tcgccctcgg agtcggcgtc ctgggacgag aagaaccctc cgtcgggatg
     1381 gtgaaggtcg cgcaggaggt actcgagcgt gcggacgcac acctcgcggt agtgctggcg
     1441 ctcggggaag gcctggtacg cgtgcaggta cgcgcgcgcg agcagggcgt tgtcgcagag
     1501 catcttctcg aaatggggga cgagccaccg ctcgtcggtc gagtagcggt ggaagccgcc
     1561 gccgacgtga tcgtggatcc ccccggccgc catgcggtcg agcgtgtgcg tcacgaggcg
     1621 ctccgcgccc gggaagccgc gccggtgcat ccgcaggagg aacccgaggt tcatcggctg
     1681 cgggaacttc ggcgccccgc cgaacccgcc ccacgtccgg tcgaaggagc gctcgagcga
     1741 gcgcgcggcc tcgtcgagga tccccgcctc gagcggctcg tccgcgacgg ggagcgaccg
     1801 cgcgatcgcc tcgctcaccc gctgcccctg cgtgacgacc tgctcccgct gctcctccca
     1861 cgcgcgggcc accgccgcca gcaccgatcg gaaccccggc atcccgtgcc gatcctccgg
     1921 cgggaagtag gtcccggcgt agaacggctg gaggtcgggg gtgaggaaca ccgtcatcgg
     1981 ccatccgccg tgcccggtca tggcctggac cgcgtccatg tagatcgcgt cgatgtccgg
     2041 gcgctcctcc cggtccacct tgacgcacac gaagtgctcg ttcatgagcc tcgccgtcgc
     2101 ctcgtcctcg aacgactcac gctccatcac gtggcaccag tggcacgcgg cgtagccgac
     2161 cgagagcagg atcggcttct cctcgcgctt cgcgcgagcg agcgcctcct cgccccattc
     2221 gtaccagtcg acggggttcg acgcgtgttg gaggaggtac ggactggtgg ctgccgccaa
     2281 gcggttctgc gaggtcacgc cttcactcta tccaccttgc ggcgttccac cgcgctcgcg
     2341 cagactgacg acgacatgac cgacgagggg gacgacatgg acgagcagcg gttccacgac
     2401 gcgatcctcg cgctcgagca ggcgacgcgc gaggagaagg ccccggaggc atggcgggcg
     2461 atcggcgaga ccgcgatcga ggacttctgg cgcgcgtggc cggagatccg ggcgtgggga
     2521 cagtggctgt ggcagctggt cgacgccgag cgagccgagc aggccgaacc cgatcaccgg
     2581 gccgacgtcg acacgggcgg acccggataa gccgaagggg gcggcgcggg cgccgccccc
     2641 ttcggatcgt gcgcgtctac cggacccgca ggctcgtgta gccgcaggtg gccccgttgc
     2701 acgcgcgcgc gaacacggtg ttgttcgtcg cgctcgtcgt gagcgtcccg gtgaaggcgc
     2761 cgttgttgag cgtcaccggc gccccgtacg tcacgctcgc cgggtccgcc gtcgcgggcc
     2821 cgacccccag ctggacccgt cccccgatgt agtaggggtt ccaggcgttg atgtagttgc
     2881 acgtcgcggt gctgctgacg acggcctgga agcagccgga gatcgacatg ctcgcggagt
     2941 tgaaggcgtt cagcgtcgcc ccgggcgcga actcgtcggc gacgatccgt cccttggtgt
     3001 cccgctggcg gaacatcagg tccatgcgca cctgccggtt cgcgtagtcg aacgttcccg
     3061 ggaggaacgc gaggtgagcg cagccggcga ccggggtcgc gatcccgtcc ccggggtact
     3121 ggtcgacgca cgcgccgcgc agctggaacc acggccggtt gttgtagtgg ttctggaggt
     3181 gtccgatcgg atcctccgcc caggtgaggg aggtgaagtt cgtgcgcttc gcctggagct
     3241 ggtaggtcgt ctgcgttccg tccggcttct cgatcgcgaa ggcccacgtg tatcggacca
     3301 cgtccggcgg gaccccgacc tgcgggtcga ccatgttcgt cacgttccag atgaagcgca
     3361 gacctccgtt gatcgggacg atctggccgc cgttctgggc gaggtcgatc ccggcggcct
     3421 gtgccaccgg cgtgccgttc gccccctgga acgcggtctt cgtccccccg acgctctgcg
     3481 gcgtcgtgat ggcggggaac gatgccgtcc ccgacaccgt gacgttgttc ccgctgagcg
     3541 tgaattcagg gctcaacgtg acgctgttgg cgctggcctg tccaaccaga gacagcgacc
     3601 cgagcagtgc gatcgccgtg acgagcgtga agcgtctgcg catgtccacc cccttgtggt
     3661 tcgcgcggtg tgcggctgtc gccggagggt tcgagagggg aggacgattt cctttgtgca
     3721 gctgcacaaa cttggcggcg ggaccgtcgc cggtggcggg acgcgacgga ggcgccagga
     3781 ggaaggccga gggggatggt caccaggacc acccccctcg gggcgagctt cggaggcgtc
     3841 agccgccgat caggcgcttc gacgcgtagc tgcaggtcac gccgttgcag gctcggacga
     3901 agaccgtgtt gttcgtgctg gacagccccg agatcgtcgc ggtgaaccgg ccagcgctga
     3961 gcgtcaccgg ggacccgtac gtgacgtcgg ccgggctggc gaggtggctc ccgaccccga
     4021 gctggacacg gccgccgatg tagtacgggc tccattcgtt gatgtaggtg gacatgaatc
     4081 cccgtccgag ctgcgtaacc gggtagtcgt tgtaggtcac gaccgcctgg aacgcagccg
     4141 agatcgacgc cccggcctgc aggctggact cgagcacgac gcccggaacg aagtcctccg
     4201 ccacgatgcg accgagccgg tcacgggcgc ggaacgggag gtcgacgcgg acctgcttgt
     4261 tcgccgggtc gaacgctccg ttgaggaagg tgaggtggta gcaaccggcg aggggcaggc
     4321 cctggtactg cggctcgcac gcgccgcgca gctggaagaa gtcctgctgt gaggtcgccc
     4381 ggttcacgtg gccgaccggg tcctcgaccg tggtggtcga ggccaggttc acgcgcttcg
     4441 cctggagctg gtagttcgtc tcgccgatct tgaaggacca cgtgtagcgg atcccctcga
     4501 gcagcgtctg ctccggcatg ctgctgaggt gccacgtgaa gcggagcccg tcctggatgg
     4561 gggagatcgt c
//
LOCUS       DATLGG010000127         4348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_135185, whole genome shotgun sequence.
ACCESSION   DATLGG010000127 DATLGG010000000
VERSION     DATLGG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135185"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VM638_03680"
     CDS             complement(<1..189)
                     /locus_tag="VM638_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HVM11563.1"
                     /translation="MEVPPFHRFLETHREEVLRFLVGVVGPDEADDCFQETFLAALRA
                     YPRLLDGENLRSWVLTIAY"
     gene            complement(225..995)
                     /locus_tag="VM638_03685"
     CDS             complement(225..995)
                     /locus_tag="VM638_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVM11564.1"
                     /translation="MAAAQRLLSRPYRAPTAAFRVRTEDGVVLSGSRLGEGGPPLILC
                     HGFLGWHRKRRVVDFAERLARRFTVYAFDFRGHGDSGGVCTYGDLEVLDVAAVAALAR
                     AESGADPVTVGISMGGTAVLRHAALRGGVRAVVAISAPARWDGHGSVAMRRILRLSGT
                     RSGRAVARAVGYRLSDAWDPPATPEEVVDRISPVPLIVVHGLDDHVFDVDEALRLYDR
                     AEEPKRLMLCARFGHAEDGLSASFAERIAARILDVIEE"
     gene            1043..1702
                     /locus_tag="VM638_03690"
     CDS             1043..1702
                     /locus_tag="VM638_03690"
                     /inference="COORDINATES: protein motif:HMM:NF044852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM11565.1"
                     /translation="MRRIVVLTHRQPAEILPALNALALDVKTEPPTPDTIDRLTDLEP
                     DVLLADALEDPERIFGLLSSPGAAAVRIPRVALLRRTDLERLPWPRCVEDIVFADATP
                     EELRLRFALARGRVPEDTAELIRAGPISINPETYQVTIAGRTLDLTYKEFELLRFLAA
                     TPGRVHTRVALLREVWGYDFYGGTRTVDVHIRRLRAKLGPEHEHLIETVRGVGYRAAS
                     G"
     gene            1836..2462
                     /locus_tag="VM638_03695"
     CDS             1836..2462
                     /locus_tag="VM638_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM11566.1"
                     /translation="MIADDHPMVRQGLSTQLASIPDVEVVAEVAGGREATAASLRDAV
                     DVAFLDLRMPEGDGIAAARAIREARPSTRVILFTVEVDRDRVEAAIRAGADGYLLKEA
                     GTPQLAEALARVLSGQRFIDPALVSILMDRAASAESEGRGGALSPRQREILLEVERKA
                     SDREIADRLGISPETVRTNLDRAFLRLGVATRAAAVAQARRRGLLAGA"
     gene            2540..3961
                     /gene="glnA"
                     /locus_tag="VM638_03700"
     CDS             2540..3961
                     /gene="glnA"
                     /locus_tag="VM638_03700"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HVM11567.1"
                     /translation="MTTPKDVLSLAKDADAQVVDLRFCDLPGLMQHFSVPIAQLDEDS
                     FESGFGFDGSSIRGFQEIQESDMLLLPDPEGAYIDPFLKHPTLVLHCFVKDPITGEMY
                     SRDPRGIARKAELYLQQTGIAETSYWGPECEFYIFDSIRFDQNEHEGYYHIDAIEGVW
                     NSGAEEGGRNLGYKPRYKEGYFPLPPMDHYQDLRTEMSLNIEKLGMKVEVHHHEVGTA
                     GQAEIDIRFDTLLKSADNVMKFKYAVKNTARQHGKTVTFMPKPIFQDNGSGMHSHQSL
                     WNGDENLFWDEMGYAQISDACRWYIGGLLQHAPALLAFCAPTTNSYRRLVPGYEAPIN
                     LVYSQRNRSACVRIPVYYKTPAAKRLEFRSPDPSANPYLAFSAMLMAGLDGIRNKIEP
                     PDPIDKDLYDLPPEEAVNVKQVPGSLEEVIDALEADHQFLLEGGVFTQDVIDTWIDYK
                     RTREIDQMRLRPHPYEFHLYYDI"
     gene            complement(4013..>4348)
                     /locus_tag="VM638_03705"
     CDS             complement(4013..>4348)
                     /locus_tag="VM638_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11568.1"
                     /translation="PQEANGIIEKTPPSAREVVTGFNAWGERALLIFTGGAQPDARYR
                     IVLRDLSVIEGGRTIQVFGAFRRRSGKRSSDPVVPWVVASLDVRATVNAEGCVLSFPE
                     VSAITTNCH"
BASE COUNT          728 a         1556 c         1465 g          599 t
ORIGIN      
        1 gtacgcgatc gtgagcaccc acgaccggag gttctcgccg tcgagcagcc ggggatacgc
       61 gcggagggcc gccaggaacg tctcctggaa gcagtcgtcg gcctcgtcgg gacccacgac
      121 gccgacgagg aaccgcagga cctcctcgcg atgcgtctcg aggaaccggt ggaacggcgg
      181 cacctccatg acgatggaac gaccggcgcg gcttccgcgt gaggtcactc ctcgatcacg
      241 tcgaggatgc gggcggcgat gcgttcggcg aacgaggcgc tgaggccgtc ctccgcgtgc
      301 ccgaagcggg cgcagagcat gagtcgcttc ggctcttccg cccggtcgta caggcggagc
      361 gcctcgtcga cgtcgaagac gtggtcgtcc agaccgtgca ccacgatgag cggcacgggc
      421 gagatcctgt cgacgacctc ctccggcgtc gcgggggggt cccacgcgtc ggacagtcgg
      481 tagccgacgg ccctcgccac ggcacgtccc gagcgggtgc cgctcaatcg caagatccga
      541 cgcatcgcca ccgacccgtg gccgtcccaa cgcgccggcg ccgagatcgc gacgaccgca
      601 cgcacgcccc cgcgcaacgc cgcatgccgc aggaccgcgg tccctcccat cgagatgccg
      661 acggtcaccg gatccgcccc cgactccgcg cgcgccaacg cggcgaccgc cgcgacgtcg
      721 agtacctcga ggtcgccgta cgtgcagacg cccccgctgt cgccgtggcc gcggaagtcg
      781 aaggcgtaca cggtgaagcg tcgagccagc cgttcggcga agtccacgac gcggcgcttg
      841 cggtgccagc cgaggaaccc gtggcagagg atcagcggcg gcccaccttc cccgaggcgg
      901 gagcccgaga gcacgacccc gtcttcggtc cggacccgga aggcggcggt cggtgcgcgg
      961 taggggcggg agagcaagcg ctgggcggcg gccatcggcc gcccagccta gcgtctcggc
     1021 gccctctgct accctcgctg cggtgcggcg catcgtcgtc ctgacgcatc gacagcccgc
     1081 cgagatcctc ccggcgctga acgcgctcgc gctcgacgtg aagaccgaac cgcccacccc
     1141 cgacacgatc gatcgcttga cggatctcga gccagacgtc ctgctcgcgg acgcgctcga
     1201 ggatccggag cggatcttcg gcctcctctc ctcgcccggc gccgccgcgg tgcggatccc
     1261 gcgcgtggcg ctcctccgca ggacggacct cgagcgactc ccgtggcccc gatgcgtcga
     1321 ggacatcgtc ttcgccgacg ccaccccgga agagctccgc ctgcgcttcg ccctcgcgcg
     1381 tggacgcgtc cccgaagaca cggccgagct catccgagcg gggccgatct cgatcaaccc
     1441 ggagacgtac caggtgacga tcgccggacg gacgctcgac ctgacgtaca aggagttcga
     1501 gctgctcaga ttcctcgcgg ccacgccagg ccgggtccac acccgcgtgg cgctgctccg
     1561 cgaggtgtgg ggatacgatt tctacggcgg aacccggacc gtcgacgtcc acatccgacg
     1621 tctccgcgcg aagctgggtc ccgagcacga gcacctgatc gagaccgtcc gcggcgtggg
     1681 gtatcgggcc gcgtccggtt aggccgtccg ggggacacca ccgcaggcag gggacacggc
     1741 ggccctggaa ggggccagta cgagaaccgt cccgtggagc cattcacgcc atcctccgga
     1801 gaaggcatcc tgggcccatg ccaccgatca gggtcttgat cgcagacgac catccgatgg
     1861 tccgccaggg gctgtccacc cagctcgcca gcatccccga cgtggaggtg gtggccgagg
     1921 tggcgggcgg gcgcgaggcg acggccgcga gcctgcggga cgcggtggac gtcgccttcc
     1981 tcgacctgcg gatgccggag ggcgatggca tcgcagcggc ccgggcgatc cgcgaggcgc
     2041 gcccctcgac ccgggtgatc ctgttcaccg tcgaggtcga ccgcgaccgc gtggaagcgg
     2101 cgatccgggc cggggcggac ggctacctgc tgaaagaggc gggtacgccg cagctcgcgg
     2161 aagcgctcgc acgcgtgctc tcggggcagc gcttcatcga ccccgccctc gtcagcatcc
     2221 tcatggaccg cgccgcatcg gcggagtccg aaggccgcgg cggggcgctc tctccacggc
     2281 aacgggagat cctgctcgag gtggagcgga aggcctccga ccgcgagatc gccgaccgcc
     2341 tggggatctc cccggagacc gtgcgcacga acctcgaccg cgcgttcctg cgactcggcg
     2401 tggcgacccg cgccgctgcg gtcgcgcagg cccgccgtcg aggcctcctc gccggcgcgt
     2461 aacgaagaat taacccctcc gaaaccatcg cgggcgcgga cccgcgctac cctcgcgacg
     2521 tcgaccggaa ggagggggaa tgaccacacc gaaagacgtc ctgtcgctcg cgaaggatgc
     2581 cgacgcgcag gtcgtggacc tgcggttctg cgacctgccg ggactgatgc agcacttctc
     2641 ggtcccgatc gcccagctcg acgaggactc gttcgagtcg ggcttcgggt tcgacggctc
     2701 ctcgatccgc ggcttccagg agatccagga gtcggacatg ctgctcctgc ccgaccccga
     2761 aggcgcctac atcgacccct tcctcaagca ccccacgctc gtgctccact gcttcgtgaa
     2821 ggatccgatc accggcgaga tgtattcacg cgacccccgc ggcatcgcgc ggaaggccga
     2881 gctctacctc cagcagacgg gcatcgcgga gacctcctac tggggcccgg agtgcgagtt
     2941 ctacatcttc gactcgatcc ggttcgacca gaacgagcac gagggctact accacatcga
     3001 cgccatcgag ggcgtgtgga actcgggcgc cgaggagggg ggccgcaacc tcgggtacaa
     3061 gccgcggtac aaggagggct acttccccct gcccccgatg gatcactacc aggacctgcg
     3121 gaccgagatg tcgctcaaca tcgagaagct cgggatgaag gtcgaggtgc accaccacga
     3181 ggtcggcacg gccggtcagg cggagatcga catccggttc gacacgctgc tcaagagcgc
     3241 cgacaacgtc atgaagttca agtacgcggt caagaacacg gcacgccagc acggcaagac
     3301 cgtgacgttc atgccgaagc cgatcttcca ggacaacgga tcggggatgc acagccacca
     3361 gagcctgtgg aacggcgacg agaacctgtt ctgggacgag atgggctacg cgcagatctc
     3421 cgacgcgtgc cgctggtaca tcggcggcct gctccagcac gcgccggctt tgctggcgtt
     3481 ctgcgctccg acgacgaact cgtaccgccg gctcgtgccc ggctacgagg cgccgatcaa
     3541 cctcgtgtac tcgcagcgca accgctcggc gtgcgtccgg atcccggtgt actacaagac
     3601 gccggccgcg aagcgtctcg agttccgctc ccctgatcct tcggcgaacc cgtacctcgc
     3661 gttcagcgcg atgctcatgg ccgggctcga cgggatccgg aacaagatcg agccgccgga
     3721 cccgatcgac aaggacctgt acgacctccc gccggaggag gccgtgaacg tcaagcaggt
     3781 cccgggttcg ctcgaagagg tgatcgacgc gctcgaggcg gaccaccagt tcctcctcga
     3841 gggcggggtc ttcacccagg acgtgatcga cacctggatc gactacaagc gcacacggga
     3901 gatcgaccag atgcggcttc ggccccaccc gtacgagttc cacctgtact acgacatcta
     3961 ggggagatgg atcgcggagg gccgccgggg aaccggcggc ccttcaccct cctcagtggc
     4021 agttcgtcgt gatcgcggag acctcgggga agctcaagac gcatccttcg gcgttcacgg
     4081 tcgcccgcac gtcgagggac gccacgaccc acggcacgac ggggtcggag gagcgcttcc
     4141 cgctgcgacg gcggaaggcg ccgaagacct ggatcgtgcg cccgccctcg atgaccgaga
     4201 gatcgcgcag cacgatccgg tagcgcgcgt cgggctgtgc ccctcccgtg aagatcagca
     4261 gggcccgctc tccccacgcg ttgaagccgg tgacgacctc gcgggcggac ggcggggtct
     4321 tctcgatgat cccgttcgcc tcctgcgg
//
LOCUS       DATLGG010000128         5896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_24965, whole genome shotgun sequence.
ACCESSION   DATLGG010000128 DATLGG010000000
VERSION     DATLGG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24965"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="rsmA"
                     /locus_tag="VM638_03710"
     CDS             complement(<1..904)
                     /gene="rsmA"
                     /locus_tag="VM638_03710"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HVM11569.1"
                     /translation="MTADRSSRTGSSTSPTTPSLASPPSPPGSSASGSSGSRLLSRRA
                     LQDLALRHDIRPSKALGQHFLADPNLARAIARDAGAAPGERFVEIGAGLGSLTLALAE
                     SGAEVLALELDRRLLSALGEVTAGEEHVRVVREDATRAVWSEVLGAGSWRMASNLPYN
                     VAVPVVLDLLEHAPGIDPMVIMVQREVGERLAARPGEAAFGAVSLKVAYRAEARVTRR
                     IPRQVFWPEPKVESVLVRLERRPPSVDVPERALFALVEEGFAQRRKTMANALARLGHP
                     RERGAAALRAAGLDERVRAEELGLE"
     gene            complement(795..1835)
                     /locus_tag="VM638_03715"
     CDS             complement(795..1835)
                     /locus_tag="VM638_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HVM11570.1"
                     /translation="MLLALILLGGLVVYLRLEKEVVLVVDGQPTAVRTIAGTVGDLLD
                     GEQIALGEHDRVRPPAGSPLREGMRISVDHAKEIRLVLNGSARTVHVIGRTVDDVLEQ
                     INLRAGRNAFVRPSRGAAIADGDVIEFRRAVRIRLRVDGETSDLITNAPDVGYLLDSL
                     GVILKRHDQVRPPVHASLADGDVVSVTRVRYDTRSEQTEIPFTTKTRETDRLVRGQRQ
                     VKRAGVPGLEEVRFRVRLEDGKPAARQVLSRKRVRNPVSEIVLVGTRDPNTQSGTASW
                     YHRSGMVAAHKTLPKGTQVRVTNLANGRTVTVVIDDRGPFVKDWIIDLSDDAFARLAP
                     LSTGLIRVRLEW"
     gene            complement(2013..2792)
                     /locus_tag="VM638_03720"
     CDS             complement(2013..2792)
                     /locus_tag="VM638_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVM11571.1"
                     /translation="MGAEAAAGLVDTHCHLFLMEGDPPALAERARAAGIETLVCVGVD
                     RESSERSRELADSLPGVFATAGVHPHSASAFDAAAGSAVEALLADPRVVGVGETGLDY
                     YRLLSPPDDQQRALRVHCALARETGKALVVHVREAWPDALRILDEERVERVVLHCFSG
                     EETMAEQAAARGWFCSFAGPVTYPKNEGLRRAAAVLPSDLLLVETDSPYLPPQALRGR
                     PNEPANAVAVVEAVAAARGESADAVAAATRENARRAFELPS"
     gene            complement(2793..4328)
                     /gene="metG"
                     /locus_tag="VM638_03725"
     CDS             complement(2793..4328)
                     /gene="metG"
                     /locus_tag="VM638_03725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HVM11572.1"
                     /translation="MKDTFYITTPIYYPNDVPHIGHGYTTVAADFVARYRRLRGERVF
                     FLTGTDEHGKKVPQAAREQGLEPQEFVDRMEPRWREVWDLLEISHDDYIRTTEPRHER
                     AVAKLLQAVHGNGRDDIYLGSYEGLYCVSCEAYYTEDELVDGMCPIHGKPVEAMSEEN
                     YFFRLSAYEDRLLEHYEANPSAIEPEARRNEVLSTIRGGLRDFSISRTSFAWGVPLPW
                     DPKHVCYVWFDALTNYITAAGYADDEERFGRTWPADVHFIGKDIIRFHAIYWPAMLMA
                     AGIDPPRRVWAHGFLLVGGEKMSKTKLTGIHPKQLVDHFGVDAYRYHFLRDVPFGQDG
                     NFSWESMLARYNADLANGLGNLASRVLAMLASYFGGEVPEATQEGAAGTLPATVEDVV
                     ARYDRAMGDMALSGAVAAVWDVVDAANQYLVERAPWKLAKDPAAREELASVMYASAEI
                     LRILAVLILPVMPRAARRLWSALGPAARLDDQRLPDAASWGGLEPGTRSHRAEPLFPR
                     LEE"
     gene            complement(4365..5657)
                     /locus_tag="VM638_03730"
     CDS             complement(4365..5657)
                     /locus_tag="VM638_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HVM11573.1"
                     /translation="MINIQALLERFRSSLFFKPALYVLGALVVAHGMLFADSVVNGEA
                     LPAYLRSTVDSARAMLSTIAGAIITVAAVVFSITVVSVQLASSQFSPRILRGFLRDRK
                     NQAVLGFVMGTFTYCLLILRAVRSPGENGEEAVIPNLSVAVGLLLAVLTALAILAFID
                     HSARSMQVGELINRIADEAIEQIRRLPRTEGPPEPEPPEESEEGTPIPARQRGWIQQV
                     DSGRVLASLQPGATFRQVVRTGHFVSEGTPLGFVSPAPADVDELAEALDEHFQYGPER
                     TMQEDIEFGMRQIVDVALRALSPGVNDPTSAYEVIVHLNAMLIELFQRDLPPRVVPGE
                     EGRRILRLNELTHPELLGRAFDQIRIIGARQPAVAIMLLRSLGVLRSEIERAELEHRL
                     PPVDEQARLVLEVCRRADPLPEDMQRVEAAAASGGFEV"
     gene            complement(5654..>5896)
                     /locus_tag="VM638_03735"
     CDS             complement(5654..>5896)
                     /locus_tag="VM638_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HVM11574.1"
                     /translation="VLRGRLSEVAASLSTPRGEVVLVIEGAAPSGGPDLDELVGRVED
                     LVAAGMTRRDAVGAVAESTGAPKNALYRAALGRETL"
BASE COUNT          869 a         2160 c         2034 g          833 t
ORIGIN      
        1 cctcgaggcc gagctcctcc gcgcggaccc gctcgtccag gccggccgcc ctcagcgccg
       61 cggctccccg ctcccgcgga tgcccgagcc tcgcgagcgc gttcgccatc gtcttccgtc
      121 gctgggcgaa gccctcctcc acgagcgcga acagcgctcg ctcggggacg tcgacggacg
      181 gaggccgccg ctcgaggcgg acgagcaccg actccacctt cggctcgggc cagaagacct
      241 gccgcgggat ccgcctcgtc acgcgagcct ccgcccggta cgccaccttc aggctcaccg
      301 caccgaacgc cgcctcaccc ggtcgggccg cgagccgctc cccgacctcg cgctgcacca
      361 tgatcaccat cgggtcgatg cccggcgcgt gctccagcag gtcgagcacc acggggaccg
      421 ccacgttgta cggcaggttc gaggccatgc gccacgagcc ggcgccgagc acctccgacc
      481 acaccgcgcg ggtcgcatcc tcccgaacga cccggacgtg ctcctccccc gccgtcacct
      541 cgcccagcgc cgagagcagc cgccggtcga gctcgagcgc gaggacctcg gcgccgctct
      601 ccgcgagcgc gagcgtgagg gacccgagcc ccgcgccgat ctcgacgaac cgctcgccag
      661 gcgcggcgcc cgcgtcgcgc gcgatcgccc gcgcgaggtt ggggtcggcg aggaagtgct
      721 gcccgagcgc cttgctggga cggatgtcgt ggcgaagggc cagatcctga agcgcccggc
      781 ggctcagcag ccggctacca ctcgagccgg acgcggatga gcccggtgga gaggggggcg
      841 aggcgagcga aggcgtcgtc ggagaggtcg atgatccagt ccttgacgaa cggtccgcgg
      901 tcatcgatca ccacggtaac cgttctgccg ttcgccaggt tcgtgacgcg cacctgcgtg
      961 cccttcggaa gcgtcttgtg cgcggccacc atccccgacc ggtggtacca ggaggcggtc
     1021 ccgctctggg tgttcggatc ccgcgtgccg acgaggacga tctcgctcac ggggttccgg
     1081 acccgcttcc gcgacaggac ctgccgagcg gcgggcttcc cgtcctcgag ccggacccgg
     1141 aagcgcacct cctccaggcc cggaacaccc gcccgcttga cctggcgctg tccgcgcacc
     1201 agacggtcgg tctctcgcgt cttcgtggtg aacgggatct cggtctgctc cgaccgcgtg
     1261 tcgtagcgga cgcgggtgac gctcacgacg tcgccgtcgg cgagtgacgc gtgcacgggc
     1321 ggtcggacct ggtcgtgccg cttgaggatc acgccgaggc tgtcgagcag gtagccgacg
     1381 tcgggcgcgt tcgtgatcag gtcgctcgtc tcgccgtcca cgcgcaggcg gatccggacg
     1441 gccctgcgga actcgatcac gtccccatcc gcgatcgccg ctccgcgcga cggccgcacg
     1501 aaggcgttgc gccccgctcg caggttgatc tgctcgagga cgtcgtcgac ggtgcggccg
     1561 atcacgtgga ccgttcgggc cgagccgttg aggacgaggc ggatctcctt cgcgtggtcg
     1621 acgctgatcc gcatgccctc ccgcagcggc gagcccgcgg gggggcgcac acggtcgtgc
     1681 tcgccgagag cgatctgctc gccgtcgagg aggtcaccga ccgtcccggc gatcgtccgg
     1741 accgccgtcg gctgcccgtc caccacgagc accacttcct tctcgaggcg gaggtagacg
     1801 acgaggccgc cgaggaggat gagggcgagc agcacgcgcg accggacgct cgggaccggg
     1861 agcgtgcggc ggacccgctc ccgccggggc cgtgatgcgc gcgggcggcg ctcaggcggc
     1921 aggggggacc gcatccgagg gtggatcggg tcgggcacgt tcccggaagg ctaccaaccc
     1981 gagctgggga gtgacaaacc tcgcatcccg cctcaggagg ggagctcgaa ggcccgccgg
     2041 gcgttctcgc gcgtcgcggc cgcgacggca tcggccgact cgccccgcgc cgccgccacc
     2101 gcctcgacga ccgccaccgc gttcgccggt tcgttcggcc ggccgcgcaa cgcctggggg
     2161 ggcaggtagg ggctgtcggt ctcgacgagc aggaggtccg aggggagcac ggccgcggcg
     2221 cgacggagtc cctcgttctt cgggtacgtg accggtcccg cgaacgagca gaaccatccg
     2281 cgcgccgccg cctgctccgc catcgtctcc tcgccgctga agcagtggag cacgacccgc
     2341 tcgacccgct cctcgtcgag gatccgcagg gcgtccggcc atgcctcgcg gacgtggacg
     2401 acgagggcct tccccgtctc gcgggcgagg gcgcagtgga cgcgcagcgc gcgctgctga
     2461 tcgtccggag gcgacaggag gcggtagtag tcgaggccgg tctccccgac gccgacgacg
     2521 cgcgggtcgg cgagcagagc ctcgaccgcg gagcccgcgg ccgcgtcgaa cgccgacgcc
     2581 gagtgcggat gaacccccgc cgtcgcgaag acccccggaa gcgagtcggc gagctcgcgc
     2641 gaccgctcgc tcgactcccg atcgaccccg acgcagacga gggtctcgat gccggcggcg
     2701 cgcgctcgct cggcgagcgc cggcggatca ccctccatca ggaacaggtg gcagtgcgtg
     2761 tcgacgaggc ccgcggcggc ttctgccccc atctactcct cgagcctcgg gaagagcggc
     2821 tccgctcggt gcgagcgcgt gcccggctcg aggccgcccc acgacgcggc atccggcagg
     2881 cgctgatcgt cgagccgcgc ggccgggccg agcgccgacc acaggcgcct cgccgccctc
     2941 ggcatgaccg ggaggatcag gaccgcgagg atccgcagga tctctgccga ggcgtacatc
     3001 accgacgcca gctcctctcg cgcggccggg tccttcgcaa gcttccacgg cgcgcgctcg
     3061 accaggtact ggttcgccgc atcgaccacg tcccacacgg ccgcgacggc ccccgacaac
     3121 gccatgtctc ccatcgcccg gtcgtagcgc gcgacgacgt cctcgaccgt cgccggcaag
     3181 gttcccgccg caccctcctg cgtcgcctcc gggacctcgc ctccgaaata ggacgccagc
     3241 atcgcgagca cgcgactcgc gaggttgccg agaccgttcg ccaggtcggc gttgtaacga
     3301 gcgagcatcg actcccacga gaagttcccg tcctggccga acggcacgtc gcgcaagaag
     3361 tggtagcggt acgcgtcgac cccgaagtga tcgacgagct gcttcgggtg gatccccgtc
     3421 agcttcgtct tggacatctt ctcgcccccg acgagcagga acccgtgcgc ccagacccgt
     3481 cgcggcggat cgatgccggc ggccatcagc atcgccggcc agtagatcgc gtggaagcgg
     3541 atgatgtcct tgccgatgaa gtggacgtcg gccggccacg ttcgtccgaa ccgctcttcg
     3601 tcgtcggcgt acccggccgc ggtgatgtag ttcgtcagcg cgtcgaacca cacgtagcag
     3661 acgtgcttcg ggtcccacgg cagcggcacc ccccaggcga acgaggtgcg cgagatcgag
     3721 aagtcgcgca gcccgccgcg gatcgtcgac aggacctcgt tgcgccgcgc ctccggctcg
     3781 atcgccgagg ggttcgcctc gtagtgctcc agcagacggt cctcgtaggc cgagagacgg
     3841 aagaagtagt tctcctcgct catcgcctcg acgggcttgc cgtggatcgg gcacatcccg
     3901 tccacgagct cgtcctcggt gtagtacgcc tcgcacgaga cgcagtacag gccctcgtag
     3961 gaccccaggt agatgtcatc acggccgttg ccgtggaccg cctggaggag cttcgccacc
     4021 gcccgctcgt ggcggggctc cgtcgtgcgg atgtagtcgt cgtgggagat ctcgaggagg
     4081 tcccagacct ccctccagcg aggctccatc cggtcgacga actcctgcgg ctcgagcccc
     4141 tgctcccgtg ccgcctgcgg gaccttcttc ccgtgctcgt cggtcccggt caggaagaac
     4201 acgcgctccc cgcggagccg ccggtagcgg gcgacgaagt cagcggcgac ggtcgtgtag
     4261 ccgtgcccga tgtgcggcac gtcgttcggg tagtagatcg gcgtcgtgat gtagaaggtg
     4321 tccttcatcg gcgcccgcga ttctcccaca gcgatccgag ctccctagac ctcgaagcct
     4381 cccgaagcgg cggccgcctc gaccctctgc atgtcctcag gcagggggtc cgcccggcgg
     4441 cagacctcca ggacgagccg ggcctgctca tccacgggag ggaggcggtg ctccagctcc
     4501 gctctctcga tctcagagcg cagcaccccc agggatcgca gcagcatgat cgcgaccgcc
     4561 ggctgtcgag ctccgatgat cctgatctgg tcgaaggccc tgccgagcag ctcggggtgg
     4621 gtcagctcgt tgagtcgcag gattcgccgt ccttcctcgc ccggaaccac ccggggcgga
     4681 aggtcgcgct ggaacagctc gatcagcatc gcgttcaggt gaacgatgac ctcgtaggcg
     4741 ctcgtcggat cgttcacccc cggcgacagc gcgcgcaggg cgacgtcgac gatctgccgc
     4801 atcccgaact cgatgtcctc ctgcatcgtc cgctcgggcc cgtactggaa gtgctcgtcc
     4861 agcgcctcgg cgagctcgtc cacgtctgct ggagccggcg acacgaaccc gagcggtgtc
     4921 ccttccgaga cgaagtgacc cgtcctgacc acctggcgga acgtcgcccc aggctgcaag
     4981 gacgcgagga cacggcccga gtccacctgc tggatccacc ctcgctgccg tgccggtatc
     5041 ggcgtgccct cctcgctctc ctccggaggc tccggctcgg gaggaccctc cgtgcgaggc
     5101 aagcggcgga tctgctcgat ggcctcgtcc gcgatccgat tgatgagctc tcccacctgc
     5161 atggaccggg cgctgtggtc gatgaacgcg aggatcgcga gagccgtcag gacggccagc
     5221 aggagcccga ccgccacgga gaggttcggg atcacggcct cctcgccgtt ctcgcccggc
     5281 gagcgcacgg cgcgcaggat cagcaggcag taggtgaagg tccccatcac gaagccgagg
     5341 acggcctggt tcttccggtc cctgaggaac cctcggagga tccgagggct gaactggctg
     5401 gaggccagct ggaccgagac cacggtgatc gagaagacga ccgcggcaac cgtgatgatc
     5461 gccccggcga tggtcgagag catcgcccgg gcgctgtcca ccgtggagcg caggtacgct
     5521 gggagggcct cgccgttgac gacggagtcc gcgaagagca tcccgtgcgc cacgacgagg
     5581 gcgcccaaca cgtacagcgc gggcttgaag aagaggctgg accggaagcg ctcgagcagc
     5641 gcctggatgt tgatcatagg gtctctctac ccagcgccgc acggtagagc gcgttcttcg
     5701 gggcgccggt gctctcggcg acggccccga ccgcgtcccg gcgagtcatc ccggcggcga
     5761 cgaggtcctc gacgcgtccg acgagctcgt cgaggtccgg tcccccggag ggcgccgctc
     5821 cttcgatgac gagcacgacc tcgcctcggg gggtcgagag gctcgcggcg acctcggaca
     5881 gccgtccgcg caggac
//
LOCUS       DATLGG010000129         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_148513, whole genome shotgun sequence.
ACCESSION   DATLGG010000129 DATLGG010000000
VERSION     DATLGG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148513"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VM638_03740"
     CDS             complement(<1..201)
                     /locus_tag="VM638_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HVM11575.1"
                     /translation="MSQGPTRESFRDVKFRIQNRVIQDLDPKLDLSNQVEVRRQIEEL
                     FGKIADEEGLALTRAERVRMLEQ"
     gene            complement(403..1659)
                     /locus_tag="VM638_03745"
     CDS             complement(403..1659)
                     /locus_tag="VM638_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM11576.1"
                     /translation="MPDQIRVLIVDDIPETRDHLTKLLGFESDVEVVGSAASGEEAIE
                     LASRLNPDVVLMDINMPGMDGIATTEQLASRAPNAAVVMMSVQGEADYLRRSMLAGAR
                     EFLVKPFSSDELIGSIRQVHTRERDKRSRIGAAQPAAGGPGAAAADSGREPAMVVAVF
                     SPKGGVGRTTVAVNMAVALATDLGRKVVLVDGSFQFGDVGVLLNLNPKNKSIADLVPE
                     LEAGEPESLDTFVINHSAGIRVLLAPPSPEMAELVTPTAVKRVLEALRRDNDFVIVDC
                     TSSFNDTTLAILDAADVILTMLSLEITSIKNMRLFLEVADQLGYEPGKVRLVLNRADS
                     TLGIRVTDVEQSIGRKVDHTIVSDGRSVVYALNRGVPFYLSNREAQVSQDILRLATAV
                     ARGHVEAPVAAAERKPAPKKSLFAWR"
     gene            complement(1773..>2176)
                     /locus_tag="VM638_03750"
     CDS             complement(1773..>2176)
                     /locus_tag="VM638_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11577.1"
                     /translation="SGPSRSRDQSFLHRPPPPPAPAEGGEGTTDPGTGTALNDQREIV
                     ILSANAQQSEVIKHAQTQVGSSMSLVLRSPGDFRDPATTEPIIPAPEDTTGITLSVLV
                     NEYDVLVPQVIEALLPDREPGQPAAPEEETP"
BASE COUNT          313 a          728 c          768 g          367 t
ORIGIN      
        1 ctgctcgagc atccgcacgc gctccgcgcg ggtgagggcc aggccctcct cgtcggcgat
       61 cttcccgaac agctcctcga tctggcgccg gacctcgacc tggttggaca ggtcgagctt
      121 cgggtcgagg tcctggatga cccggttctg gatccggaac ttcacgtcgc ggaacgactc
      181 acgcgtcggg ccctggctca tcagccgcgg cgcctgggtc ggcgggggac cgccggccgg
      241 cggtgccgga ggcaccggtg cgcccggtac ggccggcact cccggcgtgg ctcccgggcc
      301 agccgcgggc gctgcgcccg gacgcgcgct ctcgatgcgc ttcaacagtg acattcgact
      361 actccccttc tcgccctgag ggacctggag tgatgaaccg gatcatcgcc atgcgaagag
      421 tgacttcttt ggagccggct tccgctcggc ggccgccacc ggtgcctcga cgtgcccccg
      481 ggccacggcc gtggccaggc ggaggatgtc ctgcgacacc tgcgcttcgc ggttgctgag
      541 gtagaacggt acgccgcggt tgagcgcgta gaccacgctc cgcccgtcgc tcacgatcgt
      601 gtgatcgacc ttccggccga tcgactgctc gacgtcggtc acccggatcc ccagggtgga
      661 gtcggcgcga ttgaggacca ggcggacctt tccgggctcg tagcccagct ggtcggccac
      721 ctcgaggaac aggcgcatgt tcttgatgct cgtgatctcg aggctcagca tcgtgaggat
      781 cacgtccgcc gcgtccagga tcgcgagcgt cgtgtcgttg aagctcgacg tgcagtccac
      841 gatcacgaag tcgttgtcgc gccgaagggc ctcgaggacc cgcttgaccg ccgtcggtgt
      901 cacgagctcg gccatctcgg gcgaaggggg tgcgagcagc acccggatcc cggccgagtg
      961 gttgatgacg aaggtgtcga gtgactccgg ctccccggcc tcgagctcgg gcaccaggtc
     1021 ggcaatcgac ttgttcttcg gattgaggtt cagcaggacg cccacgtccc cgaactggaa
     1081 cgagccgtcg acgaggacca ccttgcggcc aaggtcggtt gccaacgcga cggccatgtt
     1141 caccgcgacc gtggtccgtc ccacgccgcc cttcgggctg aacaccgcga cgaccatggc
     1201 cggttcccgg cctgagtcgg cggccgcagc accgggccca ccggccgctg gttgagcagc
     1261 gccgatccgc gagcgcttgt cgcgctctcg cgtgtgaacc tggcggatgg acccgatcag
     1321 ctcatcgctg ctgaatggct tgacgaggaa ctcccgcgcg ccggcgagca tcgaccggcg
     1381 gaggtagtcc gcctcgccct ggacggacat catcacgacc gccgcgttcg gggcccgcga
     1441 cgcgagctgc tccgtggtcg cgatgccgtc catgcccggc atgttgatgt ccatcaacac
     1501 gacgtccggg ttgaggcgac tcgccagctc gatcgcctcc tcgccggacg cggccgaccc
     1561 gaccacttcg acgtcactct cgaagccgag cagcttggtg agatggtcgc gggtctccgg
     1621 aatgtcgtcg acgatgagga cgcgaatctg gtcgggcatg gatgacctgg tcgttctgcc
     1681 tgtctgcgga tgcggggtgg gacgtgggag tcgggcgagc ctggaggccg tgggccggct
     1741 cccgctggga ggtcgcgcgc gaccggtgac tactagggcg tctcctcctc cggcgccgcg
     1801 ggctgcccgg gctcacgatc gggcaggagc gcttcgatca cctgtggaac gagcacgtcg
     1861 tactcgttca cgagcacgga cagcgtgatg ccggtggtgt cttcgggagc cgggatgatc
     1921 ggctcggtgg tggcaggatc acggaagtcg ccgggggatc ggaggaccag ggacatcgag
     1981 ctcccgacct gggtctgggc gtgcttgatg acctcggatt gctgcgcgtt ggcggagaga
     2041 atgacaatct cgcgctggtc gttcagggcc gtcccggtcc cgggatcagt cgttccttca
     2101 ccgccctccg ccggcgcggg cggcggcgga gggcggtgaa ggaacgactg atcccgggac
     2161 cgggacggcc ctgaac
//
LOCUS       DATLGG010000130         2810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_135374, whole genome shotgun sequence.
ACCESSION   DATLGG010000130 DATLGG010000000
VERSION     DATLGG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135374"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VM638_03755"
     CDS             <1..584
                     /locus_tag="VM638_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM11578.1"
                     /translation="VELFDWRRGFKFSTYATWWIRQAITRAIADRGRQIRIPVHVQER
                     LRKVRQARERLVQKTGREPTPEELAKEADVPVEALEELETASRQEPRSLQEPVGEDAE
                     LGDLVAQMETDNPVDAVEDTMMREEIAQAVATTLEPLERRVVGLRYGLGNGRTMSLRE
                     VGEAVGVSAETVRKVEREALRKLRDAELLQGIW"
     gene            589..1434
                     /locus_tag="VM638_03760"
     CDS             589..1434
                     /locus_tag="VM638_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HVM11579.1"
                     /translation="MPVAVIGAGGAVGRALVPRLVPRATEVRALVRDPAVARSLRTAG
                     AKVAVGDVDDRDTLVAVLYDAHTVCHLVGGLFVEEGEHRERNLESTLAVVEAAAASNV
                     RRLLFPSFPGADAGAANAFLRAKGEAEEAIRGSGIEHVIVRSTHLVGPSTAWPREIAR
                     LARRRPSVVPGTGRQRVAPAGLEDLVAVLVAADDRASPPAGTFGLQGPQTLTLDEVAD
                     RLAGPRRWRRHTDRPPGVAPTAAEVLSADCVADAPDALAAFGLEATPVLRGSPDAHPP
                     GGQNG"
     gene            1526..2701
                     /locus_tag="VM638_03765"
     CDS             1526..2701
                     /locus_tag="VM638_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVM11580.1"
                     /translation="MNEIVTLALRAVRHPQTGLAAVLSAASADLHGRIGAIYLAPAGP
                     GPVELVAAAGRDIDRIGSPGAVHGRTSEVVRVLVLQTTAEWGPTPTGQPEPWSSYPYQ
                     GRVSTPLPSGGACLLLIAGEAPNDPELLREVSAPIALLAGALASARELEALRSELHAV
                     RQSKTLMAAGLQHDLRAPLTSILGCARTLRTQGQLLSEEERAELLDIVASQSERMNGM
                     VSEALSSDPIAEDSPLRRSAVSTQEVAARAAAAARVGRGGDVDIQVADDRLVTDSNRL
                     ERVLLNLLDNALKYSPEGNPVHLLGSWRDDEYVFVVADAGPGVTPSVVPTLFSAYATD
                     PHRSGGIGLGLHSVATLARDLGGRVAYARKEGWTRFCVTIPDMARDAAPIGAEEVPA"
BASE COUNT          380 a         1009 c         1044 g          377 t
ORIGIN      
        1 gggtcgagct cttcgactgg cgacggggct tcaagttctc cacgtacgcg acgtggtgga
       61 tccgccaggc gatcacccgc gcgatcgccg accgcggccg ccagatccgg atccccgtcc
      121 acgtccagga gcgcctgcgg aaggtccggc aggctaggga gcggctcgtg cagaagacgg
      181 gccgggagcc gacccccgaa gagctcgcga aggaagccga cgtccccgtc gaggctctcg
      241 aggagctgga gacggcctcg cgccaggagc cacgctccct ccaggagccc gtcggcgagg
      301 atgcggagct cggggacctc gtcgcgcaga tggagaccga caacccggtc gacgcggtcg
      361 aggacacgat gatgcgcgag gagatcgcgc aggccgtcgc aacgacgctc gagccgctcg
      421 agcgccgcgt cgtggggctc cggtacggcc tcgggaacgg ccggacgatg agcctgcgcg
      481 aggtggggga ggcggtcggg gtctccgccg agaccgtgcg caaggtggag cgcgaggcgc
      541 tccggaagct gcgcgacgcg gagctgctcc agggcatctg gtgaggccgt gccggtcgcg
      601 gtgatcggcg cgggcggcgc cgtcggccgc gcgctcgtcc ctcgtctggt tccgcgcgcg
      661 accgaggtcc gcgccctcgt ccgggacccc gcggtcgccc ggtcgctccg cacggccggg
      721 gcgaaggtcg cggtgggcga cgtggacgac cgcgacaccc tcgtcgcggt gctctacgac
      781 gcccataccg tctgccacct cgtcgggggg ctcttcgtcg aagaagggga gcaccgcgag
      841 cggaacctcg agtccaccct cgcggtcgtc gaggccgccg ccgcgtcgaa cgttcgacgc
      901 ctgctcttcc cgtcgttccc cggcgccgac gctggcgcgg cgaacgcctt cctccgcgcc
      961 aagggggaag ccgaggaggc gatccgcgga tcggggatcg agcacgtgat cgtccgctcc
     1021 acgcacctcg tcgggccatc gaccgcgtgg ccgcgggaga tcgcgcggct cgcccgacgc
     1081 agaccctcgg tggtcccggg gacgggccgc cagcgcgtcg ctcctgcggg gctcgaggac
     1141 ctcgtggcgg tgctcgtcgc cgccgacgac cgggcgtcac cgcccgcggg cacgttcggc
     1201 ctccagggac cgcagacgct aaccctcgac gaggtggcgg atcgcctcgc cggcccgcgc
     1261 cgctggcgcc gccacaccga ccggcccccc ggggtggcac cgaccgccgc cgaggtcctg
     1321 tccgcagact gcgtggccga cgcccccgac gcgctcgcgg ccttcgggct cgaggcgacg
     1381 cccgtcctgc gcggttcgcc cgatgcgcac cccccgggtg ggcagaacgg gtgaccgaag
     1441 atggccacaa ggggctactc aataggtccc tccagatgac cgattctcca gaggagatga
     1501 ggcgccatgt cgggcggagg tcctcgtgaa cgagatcgtg acgctggcgc ttcgagcggt
     1561 ccgtcatccc cagacggggt tggcggcggt gctttcggcg gcgagcgccg atctgcacgg
     1621 gcgcatcgga gcgatctacc tcgcgcctgc cggtcctggg ccggtcgagc tggtcgcggc
     1681 cgcgggcaga gacatcgatc gcatcgggtc accgggagcg gtccatggtc ggacgtccga
     1741 ggtcgtccgc gtcctcgtgc tgcagacgac cgcggagtgg ggccccaccc ccacgggaca
     1801 gcccgagccg tggagctcct acccgtacca ggggcgcgtc tcgaccccct tgccctccgg
     1861 cggcgcctgc ctgctcttga tcgcggggga agcgccgaac gaccccgaac tgctccgtga
     1921 ggtctcggcg cccatcgcgc tgctcgccgg tgcgctcgcc tcggcacgtg agctggaggc
     1981 gctgcgcagt gagctgcacg cggtgcgcca gtcgaagacg ttgatggccg ccgggctcca
     2041 gcacgatctc cgggcgccgc tcacgtcgat cctcggctgc gcgcggacgc tgcggacgca
     2101 gggacagctg ttgagcgagg aggaacgcgc ggagctgctc gacatcgtcg cgtcgcagtc
     2161 ggagcggatg aacggcatgg tgtccgaggc gctgagctcc gaccccatcg ccgaggacag
     2221 cccgctgcgg cgatcggcgg tgtcgaccca ggaagtcgcg gctcgtgccg ctgcggcggc
     2281 acgcgtgggg cgcggcggcg acgtggacat ccaggtggcc gacgaccgtc tcgtgacgga
     2341 ctcgaaccgc ctcgagcggg tgctgctcaa cctgctggac aatgccctca agtacagccc
     2401 cgagggcaac ccggtgcacc tcctgggctc gtggcgggac gacgagtacg tgttcgtcgt
     2461 ggccgacgcc ggtcccggcg tcacgccatc cgtcgtgccg acgctgttca gcgcgtacgc
     2521 gaccgacccc caccggagcg gtggcatcgg ccttggcctc cactcggtcg cgacgctcgc
     2581 gcgcgacctc ggcggacgtg tcgcctacgc ccgcaaggag ggctggacga ggttctgcgt
     2641 gacgatcccc gacatggcgc gcgacgcggc cccgatcggc gcggaggagg tgccggcatg
     2701 atgctcgacg tcatcgccgg gcgcctcggc gcgagcgaca tgttcgtgtt ccaccgtgtg
     2761 gagcacagcc gcctcgtcaa cctcgacggc tacgggcgcg gggccgggtg
//
LOCUS       DATLGG010000131        12132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_135385, whole genome shotgun sequence.
ACCESSION   DATLGG010000131 DATLGG010000000
VERSION     DATLGG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135385"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2581
                     /gene="ligD"
                     /locus_tag="VM638_03770"
     CDS             137..2581
                     /gene="ligD"
                     /locus_tag="VM638_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVM11581.1"
                     /translation="MPRRRDAFTVPTPVPLPAEREGEHWWIEVSNQRVRLSNLDKVYW
                     PEEGFTKGDLLAYYYNVADVILPYLADRPLTMKRMPDGVGGSHFYEKNAPSHTPDWMP
                     RCRVESEEDEAGMGYNDFLMANSVAGLLFVANLGAIEFHPLHSRCGTIERPDYLFFDL
                     DPMGAATFDDVLAVAQHVRAALGALNLPAYPKTSGATGLQIYVPLADALGYDAVRSFV
                     GTVGQAIRRADPDRVTMTWDTRKRGAKIFIDHNMNRRGANISAVYSVRPEPGASVSTP
                     LTWEEIERGDISPRDFTMENIHERLAEVGDLFRPVLDDPVHVREAMRAVGVDPGPEPA
                     TDEGRSLAEYERKRDFGGTPEPPPAIPDRPGNSFVIQKHDATRLHYDLRLERGGVLVS
                     WAVPKGLPLEPGERRLAVQTEDHPLDYGSFEGWIPEGHYGAGEVKIYDRGTYEPIEWR
                     HDKVTFRLHGERHRGEYHLVKTRENWLVFLSRRSAEEQASGAPDLRPMLAEPWPEPFD
                     DERWRFEPKLDGIRALAYIRTDGTRLVSRTGRDQTAQYPELANLARFVNARDAVIDGE
                     IVALDEEGRPSFERLQQRMNLTSPRDVERARRDVPVLLYAFDVLWVDGRDLTGEPLEE
                     RRAELERIATEIGVLRLTTVIDGRGTDLFAAAKDLGFEGILAKRLGSRYAPGRRSKEW
                     RKIKAVNRQDCVILGWTRGEGARSSTFGSLLVGAYRREEGDLVWIGQVGTGFTAALLD
                     DLLARLRSIESDDPPIEDRSLRAVKGARWVRPELVCDVEYLQMTKAGKLRAPSFKGLR
                     PDKRPSECLLEPPAPS"
     gene            complement(2574..3080)
                     /locus_tag="VM638_03775"
     CDS             complement(2574..3080)
                     /locus_tag="VM638_03775"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HVM11582.1"
                     /translation="MSEEEREFEPLDDEERQNIEADLRDLEGMRAAFEPQGTKGVVIA
                     CPECGSDHYYGWDLLRESLEHMLRSGEPRMHEPAFEPREEDYVLWDYGKGYVDALADT
                     GLDSGDHIEMTSCPWCQTPVEPHFGYCPSCGRSLAPGRLLQELLTRGLDEREARALLV
                     RAGFEPFS"
     gene            complement(3160..3489)
                     /locus_tag="VM638_03780"
     CDS             complement(3160..3489)
                     /locus_tag="VM638_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11583.1"
                     /translation="MSFEEALRRRGFAPVRQSRGIRHFARKPNDFLTYWVQIRPDGTA
                     LFSWEFAIAEYVSTLGLQVGSDEHLNTFLYPKVDERGPQDPAWLTGCIDRAEAVLESV
                     SLLEAGS"
     gene            3580..6261
                     /locus_tag="VM638_03785"
     CDS             3580..6261
                     /locus_tag="VM638_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVM11584.1"
                     /translation="MVRTLSSGLRVRLVALTLLAVVPALGVILYTNVQHHDRAASAAR
                     ADLRRAALLVEAHHEQVVGSARHLLVALRQTREVSEGGSGCARALREIVRRDARYANL
                     GVVDTNGLLRCSARNHRGVPPDLTGFVGSTLRAGAPEAARFAPEPVTRRPSLLLSSPI
                     LDEEGRVRGAMFAAIDLDWLSIAIGGSRLPDGATVTIVDDSGTVLVRLPQQARWVGRR
                     SVDAPLIRTMLAGSATAEVPDLDGVQRLYAFARIGSGERSQGHIAVGIPASAAFAEAH
                     RILAGALIVFGLAALLAIGAAWLGGDVFILRPVRLMERRAAAEERRRVEEERREAEER
                     FRAAFDNAPIGMALTTVEGRYLQANRALGELLGRSPDELLHLSWQDITHPEDISPDEQ
                     LRERAIAGEISSYQIEKRYVHREGGLVWALLSVSILRDRRGEPLYFISQIEDITERRA
                     VQERLAHQALHDPLTGMPNRTLFMDRLEVALHRLRRTRGIAAVLFLDLDRFKVVNDSL
                     GHDAGDRVLVAVSERLRTVVRPADTVARFGGDEFVILCEGIAGSAEAETIASRIAEAL
                     EIPVELGDGEIVLTASVGIALATGPENPLALLRDADAAMYRAKDRGRARHEIFDDDLR
                     GNAVQRLDTERFLRHALERDEIRLFYEPVVELPTGSVIGVEALVRWQHPTRGLLGPGH
                     FLPVAEETGLIVPLGAWVLDEACRQARAWQELRPEGPPLTVAVNLSGRQLADENLTTQ
                     VAAAIDRNDLDPSVLFLEITEGVLMSEAGATVETLRALKELGVRISIDDFGTGYSSLS
                     YLKRFPVDIIKVDRSFVDGLGDDAEDSAIVRAIVTLAHSLGLDAVGEGVETERQLEAL
                     RGLGCDAAQGYLWSRPKPPEEIEPLLRASSPAVRPSR"
     gene            complement(6263..7285)
                     /gene="rmuC"
                     /locus_tag="VM638_03790"
     CDS             complement(6263..7285)
                     /gene="rmuC"
                     /locus_tag="VM638_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HVM11585.1"
                     /translation="MDNLIPILVLALVAGGLVSAGLTAVALRRRAPADPTPLLEAQGL
                     RIDRLADALARRDREEIDLRGDLGRTREVVEALRAHADEQRRSAEVGWEVVRRLEAVL
                     VGGGARGRSGENVLHEALAALPPDMVVRDFRVNGRVVEFALCLPDGRRLPVDSKWAAV
                     RELEALETTEDAARRDALARDVERCVAKRAAEVAAYLDPALTTPFAVACIPDPAFAVC
                     RRAHGEAFARGVVLVPYQTALPVLLALYSLAMRHGGASDIGACLSDLEALLDAMEQTL
                     ENKLARAGTMLANATGEWRTQLGKARGAVARGRGVSGLEPGDASAGELRAEGEAIARV
                     WPLASS"
     gene            complement(7408..8175)
                     /locus_tag="VM638_03795"
     CDS             complement(7408..8175)
                     /locus_tag="VM638_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11586.1"
                     /translation="MRRTPLLLSLLLVCSIGLAPSPATAAPGEELLHSVTGNIKMFTR
                     FTDGEQGFPGVGRRLYQAARETNGLVSYTFRVDPATWMGRFELIVTGESGIGDAADLG
                     IYFYEDLADAGNSAAVTTASYDVRKPGGEIGYIAPGSRYAIVFMSRGINVDFAYRGFS
                     PMTVEIRDTGFVPADVTVGEGGWIVWKNVGQNFHSVTAPGRFDSSPGTNRPLIPGATF
                     ARQFDTVSTISYMDKYNPEASGTIRVVPGPGPGTPAA"
     gene            complement(8260..9081)
                     /locus_tag="VM638_03800"
     CDS             complement(8260..9081)
                     /locus_tag="VM638_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVM11587.1"
                     /translation="MVASRARGADRRALRGPSHALRSAAVSPAAADRDRGRVLIQVRR
                     DAEIYTKEGGWFSARWHFSFDDYRDPDNMGFGNLRVFNDDRLVPGAIWPLHPHRDIEG
                     LTYVVEGSFGHQDDVGGDFGPLPAGSVQRMTLGSGAWHSEQNASQTEPMRFIQMWIIP
                     EQRGLRPGVEQRVFTTEDRTDRLLPAITPEGGDAVKVHGNASVYVSRLTAGAQVDHRI
                     PDRMGAYLYVIRGDLALGVDRLGTGDAAKIRDELEIRLRAEAETELILVEVDLSR"
     gene            complement(8964..9896)
                     /locus_tag="VM638_03805"
     CDS             complement(8964..9896)
                     /locus_tag="VM638_03805"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVM11588.1"
                     /translation="MGMLEGKVVAVTGAGRGIGRGEALLLATEGAAVVVNDLGGEWDG
                     TGEDTRVASEVVREIESRGGRAVGHYADISEPEGAASLISTALETWGRIDAVVNNAGI
                     LRDRMSFNMSVEDWDAVIKVHLRGTFLCTREACAHWRARAKTGEEVSGRIVNTSSTSG
                     LLGIAGQSNYGAAKAAIAALTQIVSMEMSRYDVTINAVAPGARTRMTEETFGELKVEG
                     EFDPLEPGNVAPLVAYLCSDASRHITGQIFNATGGIVQLYQGWTPVAEIDKGARWSPA
                     ELAERIGELFGDRPTRYDPPPSPLRRLTGIGGAS"
     gene            complement(10159..>12132)
                     /locus_tag="VM638_03810"
     CDS             complement(10159..>12132)
                     /locus_tag="VM638_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11589.1"
                     /translation="EVYSLKPYSPASEPEVKAFGKVLRDIRRSTSAGRFAGGIDLHGM
                     ITAYAFSYTLLGAGQRDYRKNALSVDTSIRTWVDQTRRMGWSPYVADANANGAVDTGE
                     VCVSEPVLGGSSTRGRVPACVADQWGTVIDTIGYQVTGSMSDWFDSRLGLDGVGIGNE
                     MYASHLAPNIVFDPALEQTHIDGNKGLIYSQIAALLTSGEVTYEPPGRIAYVHNPKRL
                     RVNEVLRTKNPGLPAQEDIDVLIPCQDAVAQNLEGGCDGGKLVLAPSGAPAGMSFEFD
                     VKGPAEGIWNGGITATLTYPSAAGVGRGELQGMQLQYNDEGQWQTIGYHFRQGMGSSG
                     NHPGSLPSSTNNTDLYLPAGQVVTANDPKPGRYRIFTQVATGTPRLKIDFNAAEAEES
                     AGQVKIDASSMDFFDDLNRSVPEGSKLEPVTVDDIVAAPGSLDAYDSLIVVNRMGDRA
                     FVENDLGHGSATAGAYFAALKRFAQEGGNLVLTDAALRTLPELGIVPAGALRSATGTH
                     ANFAFQLGSTLSYTDPERFPLATDVMKPGAAEQETGRRQAVEPTPLGYTPATGLDATP
                     LMTTWGVDRTAWNAACGLPDCSVALTASGGTTTNLGEAVLGRGRVRIAGTMFPDPTYS
                     PDTYNDHRFGVASYALTYTAYEVFENLVDHRRR"
BASE COUNT         1760 a         4345 c         4247 g         1780 t
ORIGIN      
        1 cgctcctcgt cgccgcgatc ctggcgatcg gcacgaacat ggtcgtggaa caacggaagc
       61 gggatctcga cgagctccgg gctgtcccga tcacggtctc cccggagccg acgccggcgc
      121 ccggcggctc ggactagtgc cgcgacgccg cgacgcgttc acggtcccga cgccggttcc
      181 gctccccgcc gagcgcgaag gagagcactg gtggatcgag gtgtcgaacc aacgggtccg
      241 tctctcgaac ctcgacaagg tctactggcc ggaggagggg ttcacgaagg gggacctgct
      301 cgcctactac tacaacgtcg ccgacgtgat cctcccctac ctcgccgacc ggccgctcac
      361 catgaagcgc atgcccgacg gcgtcggcgg ctcccacttc tacgagaaga acgcgccgtc
      421 gcacacgccc gactggatgc cccgctgccg ggtcgagagc gaggaggacg aggcggggat
      481 gggctacaac gacttcctga tggcgaactc ggtcgcgggg ctgctgttcg tcgcgaacct
      541 cggagcgatc gagttccatc ccctgcactc gcggtgcggg acgatcgagc gtcccgacta
      601 cctcttcttc gacctcgacc cgatgggcgc ggcgacgttc gacgacgtgc tcgcggtcgc
      661 ccagcacgtt cgggcggcgc tcggcgccct gaacctgccc gcctacccga agacctccgg
      721 cgccaccgga ctgcagatct acgtcccctt ggccgacgcg ctcggctacg acgccgtccg
      781 ctcgttcgtc gggaccgtcg gtcaagcgat ccggcgcgcc gatccggacc gcgtgacgat
      841 gacgtgggac acgcgcaagc gcggcgccaa gatcttcatc gaccacaaca tgaaccggcg
      901 tggcgcgaac atctcggcgg tctactccgt ccggccggag ccgggcgcga gcgtctcgac
      961 gccgctgacg tgggaggaga tcgagcgcgg ggacatctcg ccgcgcgact tcacgatgga
     1021 gaacatccac gagcggctcg ccgaggtggg tgatctgttc cggccggtgc tcgacgaccc
     1081 ggtccacgtc cgcgaggcga tgcgcgcggt gggcgtcgac cccggtccgg agcccgcgac
     1141 cgacgaggga cgatcgctcg ccgagtacga gcggaagcgg gacttcggcg gcacgcccga
     1201 gccgccgccg gcgatcccgg accgccccgg gaacagcttc gtgatccaga agcacgacgc
     1261 gacccgcctt cactacgacc tccgcctgga acgcggcggg gtgctcgtct cctgggccgt
     1321 cccgaaggga ctgccgctcg aaccgggcga acgccggctc gccgtccaaa cggaggacca
     1381 cccgctcgac tacggctcgt tcgagggctg gatcccggag gggcactacg gcgccggcga
     1441 ggtgaagatc tacgaccggg gcacctacga gccgatcgaa tggcgccacg acaaggtcac
     1501 gttccgcctg cacggggagc gccaccgtgg cgagtaccac ctcgtgaaga cgagggagaa
     1561 ctggctcgtc ttcctctccc gcagatcggc ggaggagcag gcgtccggcg cgccggatct
     1621 gcgtccgatg ctcgccgagc cctggccgga gccgttcgac gacgagcgat ggcggttcga
     1681 gccgaagctc gacgggatcc gggccctggc ctacatccgc accgacggga cgcggctcgt
     1741 gtcgcggacg ggacgcgacc aaacggcgca gtacccggag ctcgcgaacc tcgcgaggtt
     1801 cgtcaacgcg cgagacgcgg tgatcgacgg cgagatcgtg gcgctggacg aggaagggag
     1861 gccgtcgttc gagcggctgc agcagcgcat gaacctgacg tcgccgcgcg acgtcgagcg
     1921 cgctcgtcgt gacgttccgg tgctcctcta cgcgttcgac gtgctgtggg tcgacggacg
     1981 ggacctgacg ggcgagcccc tcgaagaacg gcgtgcggag ctggagcgga tcgccaccga
     2041 gatcggcgtc ctccgtctca cgacggtcat cgacggccga ggcaccgacc tgttcgccgc
     2101 cgcgaaagat ctcggcttcg aggggatcct cgcgaagcgg ctcggcagcc ggtacgcgcc
     2161 gggccgccgg tcgaaggagt ggcggaagat caaggcggtc aaccggcagg actgcgtgat
     2221 cctcgggtgg acccggggag agggagcgcg ctcatccacg ttcgggtccc tgctggtcgg
     2281 cgcctaccgc cgcgaggagg gagacctcgt gtggatcggg caggtgggga ccgggttcac
     2341 cgcagcgctc ctcgacgacc tcctcgcgcg gctgcggtcg atcgagagcg acgacccgcc
     2401 gatcgaggac cggtcgctcc gcgcggtcaa gggcgcccgt tgggtccgtc ccgagctcgt
     2461 ctgcgacgtc gagtacctcc agatgacgaa ggccgggaag ctccgcgccc cgtcgttcaa
     2521 gggactccgc cccgacaagc gtccgagcga gtgcctgctc gagccgcccg cacctagctg
     2581 aagggctcga acccggcacg gacgaggagg gcccgcgcct cgcgctcgtc gagaccgcgg
     2641 gtgagcagct cctggagcag gcggcccggc gcgaggctcc ggccgcagct cgggcaatac
     2701 ccgaagtgcg gctcgaccgg ggtctggcac cagggacagg acgtcatctc gatgtggtcc
     2761 cccgagtcga gccccgtatc ggcgagggca tcgacgtatc ccttgccgta gtcccacagg
     2821 acgtagtcct cctcgcgcgg ctcgaaggca ggctcgtgca tccgcggctc gccgctgcgg
     2881 agcatgtgct cgaggctctc ccggaggagg tcccagccgt agtagtgatc ggagccgcac
     2941 tcggggcagg cgatcacgac ccccttcgtg ccctgtggct cgaacgcggc gcgcatcccc
     3001 tcgaggtcgc ggaggtcggc ctcgatgttc tgtcgctcct cgtcgtcgag cggctcgaac
     3061 tcgcgctctt cctcgctcat cgtctccgtc ctccacgcca cagcggtgcg tcggcccgcc
     3121 cagcgtaccg aaggtcgcag caaccgcgcg ccggaccggt catgaccccg cctccaacag
     3181 cgaaaccgac tccagcaccg cttccgcccg gtcgatgcat cccgtgagcc aggcggggtc
     3241 ctgcggccct cgctcgtcga ccttcgggta gaggaacgtg ttgaggtgct cgtccgagcc
     3301 gacctgcagc ccgagggtcg agacgtactc ggcgatcgcg aactcccagc tgaacagcgc
     3361 ggtcccgtcc gggcggatct ggacccaata cgtcaggaag tcgttgggtt tccgggcgaa
     3421 gtggcggatg ccgcgggact gtcggaccgg agcgaagccg cgccgacgca acgcctcctc
     3481 gaagctcaca cgcggagcat acccacggcc tccacctcaa ggggacgagc tggtcaaccg
     3541 atacgaaccg ggaaggagca ccgcactcat ggtcgggaca tggtgaggac cctctcgtcg
     3601 gggctgcgcg tccggttggt cgcgctgacc ctgctcgcgg tcgtgcccgc gctgggcgtg
     3661 atcctctata cgaacgtcca gcatcacgac cgagcggcct cggcagcacg cgccgatctc
     3721 cgccgggctg ccctcctcgt ggaggcgcac cacgagcagg tggtcggaag cgcccggcac
     3781 ctactcgtcg ccctccggca gacccgcgag gtctcggagg ggggcagcgg ctgcgctcgc
     3841 gcgctccgcg agatcgttcg tcgcgacgcc cggtacgcga acctcggcgt ggtggacacc
     3901 aacggcctgc tgcggtgcag cgcgcggaac catcgaggcg tccccccgga cctcacgggc
     3961 ttcgtcggca gcacgttgcg cgccggcgca cccgaggcag cacgtttcgc gccggagccg
     4021 gtcacccggc gcccctcgct cctgctctcc agcccgatcc tcgatgagga gggacgtgtc
     4081 cgcggcgcga tgttcgccgc gatcgacctc gactggctga gcatcgccat cggcgggagc
     4141 cggcttccgg acggcgcgac ggtcacgatc gtcgacgact cggggaccgt cctcgtccga
     4201 ctcccccagc aggcgcgctg ggtgggccga cgcagcgtcg acgctccgct gatccgcacg
     4261 atgctggcag gaagcgcgac ggcggaggta ccggacctcg acggggttca acgcctgtac
     4321 gcgttcgctc gcatcgggtc cggcgagcgg tcgcagggac acatcgcggt cggtatcccg
     4381 gcctcggccg cgttcgccga ggcgcatcgg atcctcgctg gcgcactcat cgtgttcggg
     4441 ctcgccgcgc tgctcgcgat cggcgcggcg tggctcgggg gcgacgtttt catcctgcga
     4501 ccggtgcgtc tgatggagcg ccgcgccgcc gcggaggaac gacggcgggt ggaggaggag
     4561 cgtcgcgagg ccgaagagcg gttccgcgcg gccttcgaca acgcccctat cggtatggcg
     4621 ctgacgacgg tggaaggccg ctacctgcag gcgaaccgcg ccctcgggga gctcctcggg
     4681 cgctcccccg acgagctgct ccacctgtcg tggcaggaca tcacgcaccc cgaggacatc
     4741 tcgccggatg agcagctgcg cgagcgcgcg atcgcgggcg agatctcttc gtaccagatc
     4801 gagaagcggt acgtgcaccg cgagggcggg ctcgtctggg cgctgctcag cgtctcgatc
     4861 ctgcgcgacc gccggggcga gcccctgtac ttcatctcgc agatcgagga catcaccgag
     4921 cggcgagcgg tgcaggagag gctcgcccac caggcgctcc acgaccccct cacgggcatg
     4981 ccgaaccgca cgctgttcat ggaccggctg gaggtcgcgc tccaccgcct gcgacgcacg
     5041 cgcggcatcg ccgccgtcct cttcctcgac ctcgaccggt tcaaggtggt gaacgacagc
     5101 ctcggccacg acgcgggaga ccgcgtcctc gtggcggtct cggagcgcct ccgcacggtg
     5161 gtgcgtccgg cggatacggt cgcccgtttc ggtggtgacg agttcgtgat cctgtgcgag
     5221 gggatcgccg ggtcggccga ggccgagacg atcgcgagcc gcatcgcgga ggccctcgag
     5281 atccccgtcg agctgggcga tggcgagatc gtgctgacgg cgagcgtcgg gatcgcgctc
     5341 gccaccggcc ccgagaaccc gcttgccctg ctccgcgacg cggacgccgc gatgtaccgc
     5401 gcgaaggacc gcggccgggc gcggcacgag atcttcgacg acgacctccg cggcaatgcc
     5461 gtccagcgtc tggacaccga gcgcttcctc cgacacgcgc tcgagcgcga tgagatccgt
     5521 ctcttctacg agccggtcgt ggagctgccg accggcagcg tgatcggggt cgaggcgctc
     5581 gtgcgctggc agcacccgac ccgagggctg ctcgggcccg gccacttcct gccggtggcg
     5641 gaggagacag ggctgatcgt tccgctcggg gcgtgggtcc tcgacgaggc gtgccgccag
     5701 gcgcgggcct ggcaggagct gcggccggag gggccgcccc tcaccgtcgc cgtgaacctg
     5761 tccggacggc agctcgccga cgagaacctc acgacgcagg tcgccgcggc gatcgaccgc
     5821 aacgacctcg atccgtcggt cctgttcctg gagatcacag aaggggtcct gatgagcgag
     5881 gccggagcga ccgtcgagac cctgcgcgcg ctgaaggagc tcggcgtgcg gatctcgatc
     5941 gacgacttcg gaaccggcta ctcgtcgctc tcctacctca agcggttccc ggtggacatc
     6001 atcaaggtgg accgctcgtt cgtcgacggc ctcggggacg acgccgaaga ctcggcgatc
     6061 gtgcgagcga tcgtcacgct cgcacactcc ctcggcctcg acgccgtcgg tgaaggggtc
     6121 gagacggagc gtcagctcga ggcgctccgc gggctcggct gcgacgccgc ccagggatac
     6181 ctgtggagcc gcccgaagcc gcccgaggag atcgaacccc tcctgcgggc atcgtctcct
     6241 gcggtgcggc cctcgcgctg agtcagctgg aagcgagcgg ccagacccgg gcgatcgcct
     6301 cgccctccgc gcgtagctcc cccgccgatg catcccccgg ctccagcccg ctcacgccac
     6361 ggccgcgcgc gaccgctcct cgggccttcc cgagctgggt ccgccattcg cccgtggcgt
     6421 tcgcgagcat cgtgcctgcg cgggcgagct tgttctcaag ggtctgctcc atcgcgtcga
     6481 ggagcgcctc gaggtcgctg agacaggctc cgatgtcgga cgcccctccg tgccgcatcg
     6541 cgagcgagta gagcgcgagc aggacgggga gagcggtctg gtagggcacg aggaccacgc
     6601 cgcgcgcgaa cgcctccccg tgcgcccggc ggcagaccgc gaaggccgga tcggggatgc
     6661 aggcgacggc gaagggcgtc gtgagcgcgg gatcgaggta ggccgcgacc tccgccgcgc
     6721 gcttcgccac gcagcgctcg acgtcgcggg cgagggcgtc gcggcgcgcg gcatcctcgg
     6781 tggtctcgag cgcttcgagc tcgcgcaccg ccgcccattt cgagtccacc ggcagacgtc
     6841 gtccgtcggg gaggcaaagg gcgaattcga cgacgcgccc gttcacgcgg aagtcgcgca
     6901 cgaccatgtc gggtgggagc gccgcgagcg cctcgtggag cacgttctct cccgatcgcc
     6961 cacgcgcgcc gccccccacg aggaccgctt ccagccggcg cacgacctcc cagccgacct
     7021 ccgccgaccg acgctgctcg tcggcgtggg cgcgcagcgc ctcgacgacc tcgcgcgttc
     7081 gaccgaggtc gccgcggagg tcgatctctt cgcggtcacg ccgagcgagc gcatcggcga
     7141 gccggtcgat gcgcaggccc tgagcctcga ggagcggggt cgggtcggca ggcgctcggc
     7201 gtcgcagcgc gaccgcggtc agacccgctg agacgagccc gcccgcgacg agagccagaa
     7261 cgaggatggg gatgaggttg tccatgcgcc caccctagcg ggggggtctg acacgaacgc
     7321 ctgggagccc accggacccc tggcagggga cggccccggc cgcttcaacc cggccggggc
     7381 cgtgcaggac ctcctccagc ggctcgccta cgcggcgggc gtgcctggcc cggggcccgg
     7441 aacgacgcgg atcgtcccgc tggcctcggg gttgtacttg tccatgtacg agatcgtcga
     7501 gaccgtgtcg aactgccggg cgaaggtggc gccggggatc aggggccggt tcgtcccggg
     7561 gctcgagtcg aacctgccgg gcgcggtgac cgagtggaag ttctgcccca cgttcttcca
     7621 gacgatccag ccgccctcgc cgaccgtcac gtcggccggg acgaacccgg tgtcgcggat
     7681 ctccacggtc atcgggctga acccgcggta cgcgaagtcc acgttgatcc cgcgcgacat
     7741 gaagacgatc gcgtaccggc tgcccggcgc gatgtagccg atctccccgc cgggcttgcg
     7801 cacgtcgtag gacgcggtgg tgaccgccgc cgagttcccc gcgtcggcca ggtcctcgta
     7861 gaagtagatc ccgagatccg ccgcgtcgcc gatgccggac tccccggtca cgatgagctc
     7921 gaagcggccc atccacgtcg ccgggtccac ccggaacgtg taggagacga ggccgttggt
     7981 ctcccgcgcc gcctgataga ggcggcggcc cacgccgggg aagccctgct ccccgtcggt
     8041 gaaccgggtg aacatcttga tgttgccggt gacggaatgc agcagctctt caccgggcgc
     8101 agccgtggcc ggagacggcg ccaggccgat cgagcagacg agcaggagtg agagcaggag
     8161 cggcgttctg cgcatgggac gacctcctat cggcgagcct tcatccctat cgttcggcgc
     8221 ggcctgccgc ctctcctttg tgcagacgca caaaggacgt cagcgcgaga ggtccacctc
     8281 gacgaggatc agctccgtct ccgcctcggc gcggagccgg atctcgagct cgtcccggat
     8341 cttcgcggcg tcaccggtcc cgagccggtc gacgccgagg gcgaggtcgc ccctgatcac
     8401 gtagaggtac gcgcccatcc gatcggggat ccggtggtcg acctgcgcgc cggcggtcaa
     8461 gcgcgagacg tagacggacg cgttcccgtg gaccttcacc gcgtccccgc cctcgggggt
     8521 gatcgcgggg aggagccggt ccgtccgatc ttccgtggtg aagacccgct gctcgacccc
     8581 gggccggagt ccccgctgct ccgggatgat ccacatctgg atgaaccgca tcggctccgt
     8641 ctggctcgcg ttctgctccg agtgccacgc ccccgagccg agggtcatcc gctgcaccga
     8701 acccgcgggc agcggcccga agtcgccgcc gacgtcgtcc tggtgcccga aggatccctc
     8761 gaccacgtac gtgagcccct cgatgtcgcg gtgggggtgc aggggccaga tcgcgccggg
     8821 gacgagacgg tcgtcgttga acacgcgcag gttgccgaac cccatgttgt cggggtcccg
     8881 gtagtcgtcg aaggagaagt gccagcgggc ggagaaccag ccgccctcct tcgtgtagat
     8941 ctccgcgtcc cgccggacct ggatcaggac gcgcccccga tcccggtcag ccgccgcagg
     9001 ggagacggcg gcggatcgta gcgcgtggga cggtccccga agagctcgcc gatccgctcc
     9061 gcgagctcgg ctggcgacca gcgcgcgccc ttgtcgatct cggcgacggg ggtccatccc
     9121 tggtagagct ggacgatccc gcccgtggcg ttgaagatct gcccggtgat gtgccgcgag
     9181 gcgtcggagc acaggtaggc gaccagcggc gcgacgttcc cgggctcgag cgggtcgaac
     9241 tcgccttcga ccttcagctc gccgaacgtc tcctccgtca tgcgggtccg ggcacccggc
     9301 gcgacggcgt tgatcgtcac gtcgtagcgg gacatctcca tcgagacgat ctgcgtcaag
     9361 gcggcgatcg cggccttcgc cgcgccgtag ttcgactggc cggcgatccc caggagcccg
     9421 ctcgtgctcg aggtgttcac gatgcggccg ctcacctcct cgccggtctt cgcccgcgcc
     9481 cgccagtggg cgcaggcctc gcgcgtgcag aggaaggtcc cgcgcaggtg caccttgatg
     9541 accgcgtccc agtcctcgac cgacatgttg aacgacatgc ggtcgcgcag gatgccggcg
     9601 ttgttcacga ccgcgtcgat gcgtccccag gtctcgagag ccgtggagat cagcgaagcc
     9661 gcaccttcgg gttccgagat gtccgcgtag tgcccgacgg cgcgcccacc gcgggactcg
     9721 atctcgcgca cgacctcgct cgccacgcgc gtgtcctcac cggtgccgtc ccactcaccg
     9781 cccaggtcgt tgacgacgac cgccgcgccc tcggtggcga ggaggagggc ctccccccgg
     9841 ccgatccccc ggccggcgcc cgtgaccgcc acgaccttcc cctcgagcat ccccatccgc
     9901 gtcctcccgc tcgttcgctc cccgcaccct acacatcggg cgggagcgga aaccctaccg
     9961 ggaaaggggg acggccggcc cgagggccgg ccgtctgtcc atgcgccccg atcgatcggg
    10021 ctcgcctccg atcgatcagg tccgggacca ccgctcggtg gcggtggaga cctgttcggt
    10081 gctgctgtcg gaggtattcg ccgtcggcta gaccggccct ccgaccgggg gtggcggggc
    10141 gaccgccacc cccgcccctt accgcctgcg gtggtcgacg aggttctcga acacctcgta
    10201 ggccgtgtac gtcagggcgt acgacgccac tccgaaccgg tggtcgttgt acgtgtcggg
    10261 gctgtacgtc gggtcgggga acatcgtccc cgcgatccga acgcgcccgc gacccaggac
    10321 cgcctcgccg aggttcgtcg tcgttccgcc cgacgcggtg agcgccacgg agcagtcggg
    10381 aagcccgcag gccgcgttcc acgccgtccg gtcgacgccc caggtcgtca tcagaggcgt
    10441 cgcgtcgagg ccggtcgcgg gcgtgtagcc gagcggggtg ggctccaccg cctgacgccg
    10501 ccccgtctcc tgctccgccg cgcccggctt catgacgtcc gtcgccagcg ggaaccgctc
    10561 gggatcggtg taggagagcg tcgagccgag ctggaaggcg aagttggcgt gcgtcccggt
    10621 cgcgctccgg agggcgcccg ccggaacgat cccgagctcc gggagcgtgc ggagggccgc
    10681 atcggtcagg acgaggttgc ctccctcctg ggcgaaccgc ttcagggcgg cgaagtacgc
    10741 tcccgccgtc gcgctcccgt ggccgaggtc gttctcgacg aaggcgcggt cgcccatccg
    10801 gttgacgacg atcagggagt cgtaagcgtc gagggacccc ggtgcggcca cgatgtcgtc
    10861 gaccgtcacg ggctcgagct tcgacccctc gggcaccgaa cggttcaggt cgtcgaagaa
    10921 gtccatcgag gacgcgtcga tcttcacctg gccggccgac tcctcggcct cggcggcgtt
    10981 gaagtcgatc ttcaggcgag gcgtcccggt ggccacctgg gtgaagatcc ggtaccggcc
    11041 gggcttgggg tcgttcgcgg tcacgacctg accggccggc aggtacaggt cggtgttgtt
    11101 cgtgctgctc ggcagcgagc ccgggtggtt gccgctcgag cccatcccct ggcggaagtg
    11161 gtagccgatc gtctgccact gcccctcgtc gttgtactgc agctgcatgc cctgcagctc
    11221 accacgcccg acgccggccg cgctcgggta cgtgagggtc gccgtgatgc cgccgttcca
    11281 gatcccctcc gccggaccct tcacgtcgaa ctcgaaggac atcccggcgg gagcgccgct
    11341 cggagccagc acgagcttgc ccccgtcgca gccgccttcg aggttctgcg ccacggcgtc
    11401 ctggcacggg atcagcacgt cgatgtcctc ctgcgcgggg aggcccgggt tcttcgtccg
    11461 cagcacctcg ttcacccgga ggcgcttggg gttgtgcacg taggcgatcc gccccggcgg
    11521 ctcgtacgtg acctcgccgc tggtcagcaa cgccgcgatc tgcgagtaga tcaggccctt
    11581 gttcccgtcg atgtgcgtct gctcgagcgc cgggtcgaag acgatgttcg gggcgaggtg
    11641 cgacgcgtac atctcgttcc cgatcccgac cccgtccagg ccgagccggg agtcgaacca
    11701 gtcgctcatc gagcccgtga cctgatagcc gatcgtgtcg atcacggtcc cccactggtc
    11761 ggccacgcag gccggaaccc gccctcgcgt cgaagagccg ccgaggaccg gctcgctcac
    11821 gcagacctct cccgtgtcga cggcgccgtt cgcattcgcg tcggcgacgt acggggacca
    11881 gcccatccgc cgggtctggt ccacccaggt gcggatcgag gtgtcgacgc tgagcgcgtt
    11941 cttccggtag tcgcgctggc cggcgccgag caacgtgtag ctgaaggcgt acgcggtgat
    12001 catcccgtgc aggtcgatcc cgccggcgaa gcgtcccgcc gacgtggacc gccggatgtc
    12061 gcgcaggacc ttgccgaagg ccttcacctc cggctccgag gccggcgagt acggcttgag
    12121 gctgtagacc tc
//
LOCUS       DATLGG010000132         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_40830, whole genome shotgun sequence.
ACCESSION   DATLGG010000132 DATLGG010000000
VERSION     DATLGG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40830"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VM638_03815"
     CDS             complement(<1..376)
                     /locus_tag="VM638_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVM11590.1"
                     /translation="MKIEPPEPTLLVVFGASGDLTRRKLLPALYHLHHEGMLPGAFHV
                     LGYARTEMTDDAFRDLARDAVERYGRSEVDGASWRELAPKLSYRSGEFGDPGAFASLR
                     QELERLDAEHGTEGRRLFYCATP"
     gene            complement(373..1272)
                     /gene="gnd"
                     /locus_tag="VM638_03820"
     CDS             complement(373..1272)
                     /gene="gnd"
                     /locus_tag="VM638_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HVM11591.1"
                     /translation="MKIGMIGLGRMGGNMAIRLARAGHEVVGYARTQESRAAVEAEGV
                     VAVSSLEDLVAALEPPRAVWMMIPAGAVEGVLHDLTGLLDEGDLIVEGANSLFHDSVR
                     RAKAMSERAIAFVDAGVSGGVWGLEGGYCLMVGGEQAAVDRVEPALEALAPPGGWARV
                     GPSGAGHFVKMIHNGIEYGLLQAYAEGFEILESSVYDLDLAQVAELWRHGSVVRSWLL
                     DLLALALEEDPELRTVRGYVDDSGYGRWTVATAIDQEVPAPLTALSLFARFASRQDDS
                     FSAKVIAALRERFGGHAVKREDV"
     gene            complement(1269..>2716)
                     /locus_tag="VM638_03825"
     CDS             complement(1269..>2716)
                     /locus_tag="VM638_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11592.1"
                     /translation="LASLAEQDVVRRIWERDHTVWTEDQTEISDRLGWLEAVDRSREE
                     LPDLRAFVNEVRRDGLTRTILLGMGGSSLAPEVIRHAFYDGTTPTFDLSVLDTTHPDA
                     LARVETAADRALFLVASKSGTTIETRSHMSYFLDRIGDGSRFAAITDPGTPLERDAAE
                     HGFRRVFAAPRDVGGRYSALTVFGLLSAALCGVDPEMLLVEAAEATRACLPEAPIERN
                     PGAVLGAILGEAALAGRDKLTLWPSLEAAPLGAWIEQLVAESTGKRGRGILPVDDEPR
                     VDRYGDDRLFLGIGERPPHAPSVGLPFGRPAEIGALFFILEMATAVAGHVLGIHPFDQ
                     PDVQEAKERTARVLEEGAHAEEPTDGIDGLLGDVRPGDYVAIQAFVDPTPPAWERLQR
                     LRERILRETGAATTLGFGPRYLHSTGQLHKGGPATGHFVQVVDEPREDRKVPGRPYTF
                     GELIAAQAAGDLAALRARGRPIVRARIEDLA"
BASE COUNT          388 a          992 c          993 g          343 t
ORIGIN      
        1 gcggagtggc gcagtagaag aggcgccgcc cctcggtccc gtgctcggcg tcgaggcgtt
       61 cgagctcctg gcgcagggag gcgaaggcgc cggggtcgcc gaactcgccg ctccggtagg
      121 agagcttcgg ggcgagctcg cgccaggagg cgccgtcgac ctcggaccgg ccgtagcgct
      181 ccacggcgtc ccgagcgagg tcgcggaagg cgtcgtcggt catctcggtg cgggcgtagc
      241 cgaggacgtg gaaggcgccc ggcagcatcc cctcgtgatg gaggtggtag agcgcaggca
      301 ggagcttccg ccgcgtgagg tcgccggagg caccgaagac gaccagcagc gtcggttccg
      361 gcggctcgat cttcacacgt cctcccgctt caccgcgtgg ccaccgaacc gctcgcggag
      421 ggccgcgatc accttcgcgg agaaggagtc gtcctgacgg gaggcgaatc gggcgaacag
      481 cgagagagcg gtgagcggcg ccgggacctc ctggtcgatg gccgtcgcga cggtccaccg
      541 gccgtagccg gaatcgtcga cgtagccgcg gacggtccgc agctccgggt cctcctcgag
      601 cgcgagcgcg agcaggtcga ggagccacga gcggacgacg ctcccgtgcc gccagagctc
      661 cgccacctgc gcgaggtcga ggtcgtaaac cgaggactcg aggatctcga acccctcggc
      721 gtacgcctgg aggaggccgt actcgatgcc gttgtggatc atcttcacga agtgcccggc
      781 gccgctcggc ccgacgcgcg cccagccacc gggcggcgcg agcgcctcga gggccggctc
      841 gacccggtcc accgccgcct gctcaccgcc gaccatcagg cagtacccac cctcgagccc
      901 ccagacgccg ccggagacgc cggcgtcgac gaacgcgatc gcgcgctcgc tcatcgcctt
      961 cgcccgccgg accgaatcat ggaacagcga gttcgccccc tccacgatga ggtcgccctc
     1021 gtcgagcagc ccggtgaggt cgtggaggac cccctccacc gcgccggcgg ggatcatcat
     1081 ccacacggcc cgcggcggct cgagcgcggc gacgaggtcc tcgagcgagg agacggccac
     1141 gacgccctcg gcctcgaccg cggcgcgcga ctcctgcgtg cgggcgtagc cgacgacctc
     1201 gtgaccggcg cgcgccaggc ggatcgccat gttgccgccc atgcggccga gcccgatcat
     1261 cccgatcttc acgcgagatc ctcgatccgg gcgcgaacga tcggacgacc gcgcgcacgg
     1321 agcgcagcca ggtcgcccgc cgcctgcgcc gcgatcagct cgccgaacgt gtaggggcgg
     1381 cccggcacct tccggtcctc ccgcggctcg tcgaccacct ggacgaagtg accggtggcc
     1441 ggacccccct tgtggagctg gcccgtcgag tggaggtacc gcgggccgaa cccgagggtc
     1501 gtcgcggcgc ccgtctcgcg caggatccgc tcgcgcaggc gctggagccg ctcccacgcc
     1561 ggaggggtgg gatcgacgaa cgcctggatc gccacgtagt ccccgggccg gacgtccccg
     1621 aggagcccgt cgataccgtc cgtcggctcc tcggcgtgcg cgccctcctc caggacccgc
     1681 gccgttcgct ccttcgcctc ctgaacgtcc ggctggtcga acgggtggat gccgagcacg
     1741 tggccggcca ccgcggtcgc catctccagg atgaagaaga gcgccccgat ctccgcgggc
     1801 cggccgaacg ggagcccaac cgacggcgcg tggggcggtc gctcgccgat cccgaggaac
     1861 aggcggtcgt cgccgtagcg gtccacgcgc ggctcgtcgt cgacgggcag gatgccgcgg
     1921 ccgcgcttgc ccgtcgactc ggcgacgagt tgctcgatcc aggcgccgag cggcgccgct
     1981 tcgagcgacg gccagagcgt gagcttgtcg cggccggcga gcgccgcctc ccccaggatc
     2041 gcaccgagga ccgcgcccgg gttccgctcg atcggcgcct cggggaggca ggcccgcgtg
     2101 gcctcggccg cctcgacgag gagcatctcg gggtccaccc cgcagagcgc cgccgacagg
     2161 agcccgaaga ccgtgagtgc cgagtagcga ccgcccacgt cgcgcggtgc ggcgaagacg
     2221 cggcggaacc cgtgctcggc cgcgtcacgc tcgagcggcg tgccggggtc cgtgatcgcg
     2281 gcgaaccgcg agccgtcgcc gatccgatcg aggaagtagg acatgtgcga tcgcgtctcg
     2341 atcgtcgtcc ccgacttgct cgccacgagg aagagcgcgc ggtcggcagc ggtctcgacg
     2401 cgagcgagcg cgtcggggtg cgtcgtgtcg aggacgctga ggtcgaacgt cggcgtggtg
     2461 ccgtcgtaga aggcgtggcg gatcacttcg ggcgccaggc tcgagccgcc catgcccagc
     2521 aggatcgtgc gcgtcagccc gtcgcggcgg acctcgttga cgaacgcgcg gaggtcgggg
     2581 agctcttcgc gcgaccgatc gacggcctcg agccacccga ggcggtcgga gatctcggtc
     2641 tgatcctcgg tccaaaccgt gtggtcgcgc tcccagatcc gccggacgac gtcttgctcg
     2701 gcgagcgagg ccaggc
//
LOCUS       DATLGG010000133         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_179332, whole genome shotgun sequence.
ACCESSION   DATLGG010000133 DATLGG010000000
VERSION     DATLGG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179332"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VM638_03830"
     CDS             complement(<1..463)
                     /locus_tag="VM638_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVM11593.1"
                     /translation="MEISRPVPLNYDERDGLVADVAIAGGGVAGLYAAWRLLTSERRR
                     EDGEPLRVAVLEATDRIGGRIETVTFPGMPQHRAEFGAMRLASWQQLVLRLAEQLGIE
                     VAPFESGDEHNFWHLRGKRLKAEDLSDPARVPYGLEGWEVGKSPEELLEHVA"
     gene            complement(511..>2882)
                     /locus_tag="VM638_03835"
     CDS             complement(511..>2882)
                     /locus_tag="VM638_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11594.1"
                     /translation="LVQSQPEVRASIKLQREWLATAGLDLHGYVNPTLVDGLTKPHNP
                     GVEYDIFLKWNQSRLDANTVDLAGVGLPINRPVNMWSSAGAPPGHPYIAEGWDDWGPF
                     YTQTYMAYYGVDSSTVEMCSSQAGGGNCNGRLGGKVSQYVTVYSSLRMWIQNRQQMMH
                     DQLEIFRRGVTGQARPACCGEASMTARGFTEDQHNWMVESPTAYIIPFGGGGQRSDAE
                     ANRMVRWLLDNGIEVDVAREPFTYAGVTYPKGSYVVWMNQARRGLALTALSPGQDISS
                     RITQLYATPAAWSHGLLWGADVVEVPRAATLSASTDPVDEPNELEGGLKEGPADWYAV
                     TPRGVRDHQAIRGLLRGGIAGEVAEEPFTTADGASMPAGTVVFPVASGSARDALEAAA
                     EGSGLTLEPGTGPRPSSTLLREAPRVAILGNSASPAPTDTSETLKAIFGDDAGFVSVS
                     AGANSLANAPEDPLLAYDVIYNTGQGWPAAGTARDRLTAFFNRGGGYIATSQSTTGFS
                     FLSQAGLVSGSLTQSSQTAYGGIAVWNNAAGAESPVTGPYPASDFLYLPSNVTYFTAT
                     PAGSVAAGRYHSDMSGPATSPNGPSPGFVAGLWRTRGTAANSAPVIVHGSTTAGGRYF
                     ALATNPFSRQDAEREWPLIAQAALWSNLTDDPRAGASAAGTTSAELTEGFSLPLGVAV
                     GSPELPPVTGDYRGEWRASDGSWGYVDAILTRRGSLVTGRLALDAAGCDALRPARGAL
                     RGPELGLSTGTLDLDGTVEGGTISGTFSDACSGKTGTFEIYTEETRAAD"
BASE COUNT          408 a         1062 c          970 g          442 t
ORIGIN      
        1 gggcgacgtg ctcgaggagc tcctccggcg acttcccgac ctcccatcct tcgagcccgt
       61 agggaaccct ggcgggatcg gacaggtcct cggccttcaa gcgcttgccg cggaggtgcc
      121 agaagttgtg ctcgtccccg ctctcgaacg gggcgacctc gatccccagc tgctccgcca
      181 gacgcagcac gagctgctgc caggacgcca gccgcatcgc gccgaactcc gctcgatgct
      241 gcggcatccc cgggaacgtc accgtctcga tgcggccacc gatccggtcc gtcgcctcga
      301 ggaccgccac ccgtagcggt tcgccgtcct cgcgccgacg ctcgctcgtc agcagccgcc
      361 aagcggcgta gaggccggcg acgccgcccc cggcgatcgc aacgtccgcg acgaggccat
      421 cccgctcgtc gtagttcagc ggcacgggcc gcgagatctc cacctcgcca tcctagtgcc
      481 ggcgcggatc agaccatcac ggcggccgtg tcagtccgcc gcgcgcgtct cctccgtgta
      541 gatctcgaac gttcccgtct tgccgctgca ggcatcggag aaggtgccgg agatcgttcc
      601 tccctccacg gttccgtcca ggtcgagggt gccggtgctc agtccgagct ccgggccgcg
      661 cagcgctccc cgcgcaggtc gcagcgcatc gcatcccgcc gcatcgagcg cgaggcgtcc
      721 cgtcaccagg gacccccggc gagtgaggat cgcgtcgacg tacccccagg atccgtcgga
      781 ggctcgccat tcgccccggt agtcgccggt gaccggtggc agctccgggc ttcccaccgc
      841 cacgcccagc gggagcgaga agccttctgt gagctccgcg gacgtcgtcc cggcggcgct
      901 cgcccctgca cggggatcgt cggtgaggtt cgaccacagc gccgcttggg cgatcagcgg
      961 ccactcgcgt tcggcgtcct ggcgcgagaa ggggttcgtc gccagcgcga agtaccgccc
     1021 gcccgcggtc gtcgacccat gcacgatgac gggagcgctg ttcgccgccg tcccgcgcgt
     1081 ccgccacagt ccggcgacga agcccggaga ggggccgttc ggactggtgg cggggccgct
     1141 catgtcgctg tggtagcgtc ccgcagccac ggatcccgcc ggcgtcgcgg tgaagtacgt
     1201 gacgttcgac ggaaggtaca ggaagtcgct cgcggggtac gggccggtca ccggactctc
     1261 cgcacccgcc gcgttgttcc agaccgcgat gccgccgtac gcggtctggc tgctctgcgt
     1321 cagggagcct gacaccaggc ctgcctgcga gaggaacgag aagccggtcg tcgactggct
     1381 cgtggcgatg tacccgccgc cacggttgaa gaaggcggtc aaccggtcac gggccgtccc
     1441 cgccgcgggc cacccctgac cggtgttgta gatcacgtcg tacgcgagca gcggatcctc
     1501 cggtgcgttc gccagcgagt tcgcgcccgc cgagaccgac acgaaccccg cgtcgtcgcc
     1561 gaagatcgcc ttcagcgtct ccgaggtgtc ggtcggcgcg ggggaggccg agttcccgag
     1621 gatcgcgaca cgcggcgcct cccgcaggag cgtgctcgac ggccgggggc ccgtcccggg
     1681 ctcgagggtc agcccggagc cttcggcagc cgcctccagc gcatccctgg cggagcccga
     1741 agccaccggg aagacgaccg tccccgccgg catcgacgcg ccatctgcgg tcgtgaaggg
     1801 ctcctccgca acctcgccgg cgatccctcc acgcaggagg ccgcggatcg cctggtggtc
     1861 ccggacgccg cgaggcgtga ccgcgtacca gtcggcgggg ccttccttca gaccaccctc
     1921 gagctcgttc ggctcatcga ccgggtcggt ggatgcggaa agggtcgccg ccctggggac
     1981 ctcgaccacg tcggcgcccc agaggaggcc gtggctccac gccgccggcg tcgcgtagag
     2041 ctgcgtgatg cggctcgaga tgtcctgccc cggcgatagc gcggtgaggg cgagaccacg
     2101 cctcgcctgg ttcatccaga cgacgtacga ccccttcggg tacgtcacgc cggcgtacgt
     2161 gaagggctcc cgcgcgacat ccacctcgat cccgttgtcg aggagccacc gaaccatccg
     2221 attggcctca gcgtcgctgc gctggcctcc ccctccgaac gggatgatgt aggccgtcgg
     2281 gctctcgacc atccagttgt gctgatcctc cgtgaaaccg cgggcggtca tcgacgcctc
     2341 accgcagcac gcgggccgcg cctggcccgt cacgccccgc cggaagatct cgagctggtc
     2401 gtgcatcatc tgctgccggt tctggatcca catccgcagc gacgagtaga ccgtgacgta
     2461 ctgcgagacc ttcccgccga gacggccgtt gcagttgccg cctcccgcct gggagctgca
     2521 catctcgacg gtggagctgt cgacgccgta gtacgccatg taggtctgcg tgtagaaggg
     2581 gccccagtcg tcccagccct cggcgatgta cgggtggccc ggaggtgccc ccgccgagct
     2641 ccacatgttc accggacgat tgatcggcag gcccaccccg gcaaggtcca ccgtgttcgc
     2701 gtcgaggcgc gactggttcc acttgaggaa gatgtcgtac tcaacccccg ggttgtgggg
     2761 cttggtgagc ccgtcgacga gcgttgggtt gacgtacccg tgcaggtcga gcccggccgt
     2821 cgcgagccac tcccgctgga gcttgatcga tgcgcgcacc tccggctgtg actgcacgag
     2881 cc
//
LOCUS       DATLGG010000134         9802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_40986, whole genome shotgun sequence.
ACCESSION   DATLGG010000134 DATLGG010000000
VERSION     DATLGG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40986"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VM638_03840"
     CDS             complement(<1..883)
                     /locus_tag="VM638_03840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVM11595.1"
                     /translation="MSLRVRLVLTAALLALPATQAPRGPAEAPGSGTRLPATGEMMDA
                     ELPEPLSLGFISRRLPAENDADVRVNAPAAVGDPAACHIQSEATVAVDRPYVYVGFND
                     TRQCTFTLGLPGVRRSMTGFARSTDGGQTFEDLGPLVPNGPIHHLVGDPVIAVDTEGE
                     GAGTVYVSSLAHIDVYDQLLLVEPHSTVAVGVSTDRGRTFTWHDAVGERSRPGILHDK
                     EWLAVDNSGGRHDGNVYLAWSEWPADFSQVRLLFTRSSDRGQSWSTPLEIAVGFPMAP
                     QLAVGPDGEVHLAWREPQ"
     gene            complement(958..2982)
                     /locus_tag="VM638_03845"
     CDS             complement(958..2982)
                     /locus_tag="VM638_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVM11596.1"
                     /translation="MRRSIAAGILVLGLALPASAGGPAFPLRSDAPLELPTGERISGV
                     APVPGGAVALGEWTQVPRTGPVVTGGGGLAFDAARGELLTFGGCCYEGDLWGFRDGAW
                     TKHPPGLLAPGGRAGVAMVYDPIRRETVMFGASQYAETWIWDGGAWRIHHQLNGPQFR
                     NDTTMAWMPRIGKIVMFGGYPDASPDRLGKQLRDTWTWDGHEWKPVQSPVTPPSRYAA
                     ALALDPSSGDLILFGGMDDAYASLNDTWRFDGTRWSEVQTEIAPGRRSYLSMAANPAL
                     GAVELFGGYVREDFTSLGDRWFFKDREWTQELEGIENGPLPPGRIGQNLAFDDTVGRM
                     RLFGGSWCDPTCGGVWTERLEEIEREDGTLERRLVWDETPMTWPPDRMSAGTMVADPL
                     GRGTLMHGGHGDSSGSGGTNDTWLWKDGAWNRLDTHGAPPSRQGLSLGVHRGSKSVVL
                     FGGLVGYQTFDPRTWVLEGETWTPRRPETSPPGRYHAAMAEDRNGNTVLFGGLDLGSP
                     QQRLGDTWVWDGETWTQHTGPGPSPRVYPHMVYDPVRRETVLFGGSDANGVTVNETWV
                     WDGTGWEQRAPLVSPTGRLGAALAWDPAGEQVVLFGGATCVNLGCLVQSDVWTWGGSS
                     WLRREHPTGWPRPRWFTLAGPSEEGIVLFGGHDGGMGLPFSDTWLWRDPT"
     gene            complement(3064..5163)
                     /locus_tag="VM638_03850"
     CDS             complement(3064..5163)
                     /locus_tag="VM638_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11597.1"
                     /translation="MRRLLASLIVLGTLAPPASGSGEPETQRLPTSALLQQGCVDDDG
                     PPVGRLLPGLVPCGVHVAPDGSAAPAAVPPAIGSPGSASAGPGWERLRRPSPERRVNV
                     ALATFPPLDGVVMFGGLVCPGVVCNPVSETWLWQGERWRRLEPATSPPPLHAAAMTYD
                     PARKELLLFGGETCTPTECSFSSATWTFDGATWRQRETTVSPPASAASAIAYDEERRR
                     VIVTGGCAQRRCPGLVGTWSWDGAGWTLEDLLTPLVRHGASAATNPGGGIVLFGGQDV
                     RSATIGETWIWDGEWRQVSPPTSPPSRMYGGATSDGARAIFFAGSNVTCNGLGCTGSY
                     LSDMWAWNGTTWSQLQQGTAPRGGQSLAIAHDAARGEIVRFGGISNFSNYMGASTTAY
                     ESSTWIWEGDGWRERRSPMPEERIQGALGYHPGTDRLILAGGNCVYTTTCDTWAWDGE
                     RWERIGDPAPRPNSRTFLIPHAPSGAALFPTADGVWALTHDDEEERWSWVKHEDSAAP
                     VPGGSVTALDRQDSVVLFGSQSCSGSGCTYLDQTWRWAGIHWEQVPAAPRPPGRAFAA
                     MAFQPQSGNTILFGGLSSGGQVYHDDTWAWNGSTWRQIAAAGPRNRAFTRIADDPATG
                     DVLLVGGISGPPEYQVLGDHWMFDGAAWRQVPGGPGTRTEHALGGHPGTRQTLLFGGQ
                     DANPGRPMSDTWIWSGE"
     gene            5264..6178
                     /locus_tag="VM638_03855"
     CDS             5264..6178
                     /locus_tag="VM638_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVM11598.1"
                     /translation="MIGERRALASFVAFSVLAGGNAVAVRFSNRELPPLWGAGLRFVF
                     GAILLGIVMGVMRLRLPRPWDSAGAALYGLLGFGGAYALAYVALVRLHAGIGQTLLAL
                     VPLATLLLAVAWRQERLRREGVIGGVVALTGVAVISGGSVDATLPLPSVLAALGSVLC
                     IAQSAVLVRRLPPMHPVSMNTLGMAAGALLLVLLSAIVGEPHPLPTQAETWLALAYVV
                     PIGSVVVFILYLMVLRQWAASRAAYGFVIIPFVTVALSAWLDDEPLRWSLIFGGPLVL
                     VGVYLGALRTRSFAPTEAAPGYDERSAR"
     gene            complement(6183..7268)
                     /locus_tag="VM638_03860"
     CDS             complement(6183..7268)
                     /locus_tag="VM638_03860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HVM11599.1"
                     /translation="MRRTAMLVLVLVALPVFPANARQQIVGGRPATQPYPFMVSLQNN
                     SGTHFCGGSLVRPEWVLTAKHCVAGRAPSAFRVRIGSTSKSQGGQIHQIAQVITHAPS
                     ASDSAVLRLTTPSTQTPIRIGMPEEAATLWPPGTTARTIGWGTSFYLVGPSPDTLHEV
                     DVPVVSDVDCARLNGPTVGFNGAYEICAGETIGGKDACQGDSGGPLMVPDSRGRWIVV
                     GTTWKGLGCGFPLFYGSYASVGEDPIYSWLRSVLPPESSLAVSGTSGTETVGAMAFTV
                     RRTGVTARTVRVNFTTVDGSATARSDYWPVSGTLTFEPDDSTQTIFVPILEDDLTEGE
                     EWFEVRLSGATEATIQVGVATGTISDA"
     gene            7426..8511
                     /locus_tag="VM638_03865"
     CDS             7426..8511
                     /locus_tag="VM638_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11600.1"
                     /translation="MRPRASRVALATTLMASLVLPGSGLAAASDERCTDRRDAALSTR
                     PRLRLSGLYALPRKPARTLVVFAHGYRKPATVYWQRHLRREARRGAIAVAMDYRGIGG
                     APDYRGWNVRAGAADSIRAARHFWQGCRSLRRIILVSVSMGSNAAGLALAAQPVRHSS
                     PCTDRAGQPRCAAPYFPEPLFDHWVNVEGAANVTETYLEAVAVAPGNAYARAAKEDIE
                     REMGGTIAEVPERYREATLVARAEDIAASGVRSVTFVHGLEDGLVPYDQSRELAAALR
                     RHDVPSDFFTVLTRGEGDDGTTISGYALGPAGQNSPFAGHGSEEDITHLVIRTGFERL
                     WAIRRGATVGPAREFVVDGILGTHPAP"
     gene            8626..9561
                     /locus_tag="VM638_03870"
     CDS             8626..9561
                     /locus_tag="VM638_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11601.1"
                     /translation="MLAKLAIVALAFLLAVPAAAQTPGALPSLAEVELDRTAVAAGQP
                     IRVTGRLLTQVQPPARLWVDATLPGDLGSFNYPVDPQLDLVEGWVARERPGDPLTMQI
                     KVAPAIGADSGPKPYMRFRWSFHLNDLWYFVRFRYLSDGTGTGFSWQGNWYYCRLALG
                     SNCFHGDTPPFPVTWDAATRTFTGRIRLADLAAKQESGRLVQDTMRPFSSQYPPESGY
                     ELPVITIGQDSSAPFAPYDLPIETVFLGIAPVGSAPQSVNYSVAADVQTTAGSLDVPF
                     SGQISTEGLATGAYDLWVKGCFGPCVTRTFRISVS"
BASE COUNT         1361 a         3516 c         3455 g         1470 t
ORIGIN      
        1 gttggggttc gcgccatgca aggtggacct ccccatcagg cccgacggcc agctgtggcg
       61 ccatcgggaa cccgacggcg atctccagtg gggtggacca gctttggccc cggtcgctcg
      121 agcgtgtgaa cagcaaccgc acctgcgaga agtccgctgg ccactcggac cacgcgaggt
      181 aaacgttccc atcgtgccgg ccaccgctgt tgtcgacggc gagccactcc ttgtcgtgga
      241 ggatcccggg ccgggaacgc tcccccaccg cgtcgtgcca ggtgaaggtt cgtccccggt
      301 cggtggagac cccgacggcg accgtgctgt gcggctcgac gaggaggagc tggtcgtaca
      361 cgtcgatgtg agcgagtgag gacacgtaga cggtgccggc gccctccccc tccgtatcga
      421 cggcgatcac agggtcaccg acgaggtgat ggatcggtcc gttcgggacg agcgggccga
      481 ggtcttcgaa ggtttgtcca ccgtccgtcg agcgggcgaa cccggtcatg ctccgacgga
      541 cccctggcaa gccgagggtg aacgtgcatt gacgggtgtc gttgaaaccc acgtacacgt
      601 acggtcggtc gaccgcgacg gtggcttcgc tctggatgtg gcacgcggct ggatccccga
      661 cagcagccgg ggcgttgacc cggacatccg cgtcgttctc ggccggcagg cggcgagaga
      721 tgaagcccag ggaaagcggc tcggggagct ccgcgtccat catctctccc gtcgccggca
      781 gcctggttcc gcttcctggt gcctctgcag gtccacgagg ggcctgggtg gccggaaggg
      841 cgagcaacgc cgcggtgagg acgaggcgga cacggaggct caccagggga ggattcgatc
      901 cgccccccgt cgttcccttc cgggcaccgt ggcggaaggt ccgccgcatc cgcgcggtca
      961 cgttgggtcg cgccagagcc aggtgtcgct gaaaggaagg cccatcccgc cgtcgtggcc
     1021 accgaagagg acgatgccct cctcactcgg gccggccagc gtgaaccagc ggggcctcgg
     1081 ccatccggtg ggatgctcgc gacggagcca cgacgaaccg ccccaggtcc agacgtcgct
     1141 ctgcacgagg cacccgaggt tcacgcacgt cgcgccgccg aacaggacga cctgctcccc
     1201 cgccgggtcc cacgccaggg cggccccgag acgcccggtg ggcgagacga ggggcgcgcg
     1261 ctgctcccag cccgtcccgt cccacaccca ggtctcgttg accgtgacgc cgttcgcatc
     1321 gcttccgccg aacagcaccg tctcgcggcg gaccgggtcg tagaccatgt gcgggtagac
     1381 cctcgggctc ggtccgggac ccgtgtgctg ggtccacgtc tcgccgtccc acacccaggt
     1441 gtcgccgagg cgctgctggg gcgatcccag gtcgaggcca ccgaacagga cggtgttgcc
     1501 gttgcggtcc tcggccatcg cggcgtggta gcggccgggc ggcgaggtct ccggacgccg
     1561 cggtgtccaa gtctcaccct ccagcaccca cgtccgcgga tcgaaggtct ggtagccgac
     1621 cagtccgccg aacaggacca cgctcttgga tccgcggtgg acgccgaggg agaggccctg
     1681 cctggagggt ggggcgccgt gcgtgtccaa ccggttccag gcgccgtcct tccacagcca
     1741 ggtgtcgttg gtgccaccgg agccgctgct gtctccgtgg ccgccgtgca tcagcgttcc
     1801 acggccgagc ggatccgcga ccatcgttcc ggcactcatg cggtcggggg gccatgtcat
     1861 cggcgtctcg tcccacacga ggcgacgctc gagcgttccg tcctcgcgct cgatctcctc
     1921 gaggcgttcc gtccacaccc cgccgcaggt gggatcgcac cagctcccac cgaacaggcg
     1981 catccgcccc acggtgtcgt cgaaggccag gttctgaccg atccgcccag gcggcagcgg
     2041 accgttctcg atcccctcga gctcctgggt ccactcccgg tccttgaaga accagcggtc
     2101 gccgagggag gtgaagtcct cacggacgta tccgccgaac agctcgaccg cgccgagcgc
     2161 cgggttcgcc gccatcgaca ggtacgaacg acgcccggga gcgatctccg tctgaacctc
     2221 gctccatctc gtgccgtcga agcgccacgt gtcgttcagg gaggcgtagg cgtcgtccat
     2281 cccgccgaac aggatcaggt cgccgctgct ggggtccagc gcgagcgcgg ccgcgtatcg
     2341 cgaggggggt gtgacggggc tctggaccgg cttccactcg tggccgtccc acgtccaggt
     2401 gtcgcggagc tgcttcccga gccggtcggg gctggcatcg gggtaccccc cgaacatcac
     2461 gatcttgccg atccgcggca tccaggccat cgtcgtgtcg ttgcggaact gcggcccgtt
     2521 cagctggtgg tggatccgcc aggcgcctcc gtcccagatc caggtctcgg cgtactgcga
     2581 ggccccgaac atgacggtct ctcgccggat cgggtcgtag accatcgcga cccccgcccg
     2641 gccgccggga gcgaggagcc cggggggatg cttcgtccag gctccgtcac ggaagcccca
     2701 gagatcgcct tcgtagcagc atccgccgaa ggtcagcagc tcgccgcgtg ccgcatcgaa
     2761 cgcgagtccg cctccccccg tgaccacggg cccggtgcga ggaacctgcg tccattcgcc
     2821 cagcgcgacg gcgccccccg gcacgggtgc gaccccggag atccgctccc cggttgggag
     2881 ctcgagcgga gcgtccgacc ggagcgggaa cgccggccca ccggcgctcg ccggaagcgc
     2941 gagcccgagg acgaggatgc cggctgcgat cgatcggcgc atcggggttg ctttcgtcgc
     3001 gggccccgcg atcccctctg tgcagatgcc caaaggctgg ctcgcgggat cgaccggccc
     3061 gacctactcg ccggaccaga tccaggtgtc gctcatggga cggcccgggt tcgcgtcctg
     3121 gccgccgaac aggagggtct gtctcgtgcc ggggtgcccg ccgagggcgt gctcggtgcg
     3181 cgtgccgggc cctccgggga cctggcgcca ggccgcgccg tcgaacatcc agtggtcacc
     3241 gaggacctgg tactcgggcg gtccggagat gccaccgacg agcaggacgt cgcccgtggc
     3301 ggggtcgtcc gcgatcctcg tgaacgcgcg gttgcggggt ccggcggcgg cgatctgccg
     3361 ccacgtcgat ccgttccacg cccaggtgtc gtcgtggtac acctgccccc cgctcgacag
     3421 ccccccgaac aggatcgtgt tcccagactg cggctggaag gccatcgcgg cgaacgcgcg
     3481 ccccggtggc cggggtgcag ccgggacctg ttcccagtgg atgccggccc agcgccaggt
     3541 ctggtcgagg tacgtgcagc ccgatcccga gcacgactgg gaaccgaaca gcacgaccga
     3601 gtcctgacgg tccagggcgg tgacggatcc gccgggcacg ggggcggcgc tgtcctcgtg
     3661 cttgacccag gaccagcgct cctcttcgtc atcgtgcgtg agcgcccaga ccccgtcggc
     3721 ggtcgggaac agcgcggctc cgctcggcgc gtgcgggatc aggaaagtgc ggctgttcgg
     3781 gcgcggcgcc gggtccccga tccgctccca tcgctcccca tcccacgccc aggtgtcgca
     3841 cgtcgtcgtg tagacgcagt tgccgccggc gaggatcagc cggtcggttc ccgggtggta
     3901 tccgagcgct ccctggatcc gctcctccgg catcgggctg cggcgctccc tccagccatc
     3961 gccctcccag atccaggtgc tcgactcgta ggcggtggtc gaggcgccca tgtagttcga
     4021 gaagttgctg atcccgccga agcgcacgat ctctccgcgg gcggcgtcat gggcgatggc
     4081 gagcgactgc ccacctcgcg gcgcggtccc ctgctgcagc tggctccacg tcgtcccgtt
     4141 ccacgcccac atgtcactga ggtagctccc ggtgcagccg agaccgttgc aggtgacgtt
     4201 gctcccggcg aagaagatgg cgcgcgctcc atcggaggtc gcgcccccgt acatccgcga
     4261 gggcggcgat gtcggcggcg acacctgacg ccactcgccg tcccagatcc aggtctcgcc
     4321 gatcgtcgcc gagcgaacgt cctggcctcc gaacaggacg atgccaccgc cggggttcgt
     4381 cgcggccgag gcgccgtggc gaacgagcgg cgtgaggaga tcctcgaggg tccagccggc
     4441 cccgtcccag ctccaggtcc cgacgagccc cgggcagcgc cgttgcgcgc atccgcccgt
     4501 gacgatgacg cgtcgacgct cctcgtcgta ggcgatggct gatgccgcgg aggcaggtgg
     4561 ggagacggtc gtctctcgct gacgccacgt cgcaccgtcg aaggtccagg tcgccgatga
     4621 gaaggagcac tccgtcggcg tgcaggtctc tccaccgaac aggaggagct ccttccgcgc
     4681 cgggtcgtac gtcatcgccg cggcatgcag cggcgggggc gaggtggccg gctcgagccg
     4741 tcgccagcgc tctccctgcc agagccatgt ctcggacacc gggttgcaca cgacgccggg
     4801 acacacgagc cctccgaaca tcacgacgcc gtccagggga gggaacgtcg cgagcgcgac
     4861 gttgacccgc cgttcggggc tcgggcgacg gagccgctcc catcccggtc cggccgaggc
     4921 cgacccgggg ctgccgatcg ccggcgggac cgccgccgga gccgcgcttc cgtccggtgc
     4981 gacgtggaca ccgcacggga cgagacccgg caggagccgt cccacgggcg gaccatcgtc
     5041 gtcgacgcag ccctgttgga ggagggccga cgtcgggagg cgttgcgtct cgggctcgcc
     5101 ggagccgctc gcgggcggag ccagggtccc gaggacgatc agcgaagcaa ggagtcgacg
     5161 catggcgaga gattcgccga gccgagcaag gatccctttg tgcaggtgcc caacgttggc
     5221 cggccggtgc cctcgacgcg ggcgaggccg gtagcatccg ggggtgatcg gtgagcgccg
     5281 cgccctcgcg tccttcgtcg ccttctcggt gctcgccggg gggaacgcgg tcgcggtccg
     5341 gttcagcaac cgcgagctgc ctcccctatg gggagccggg ctccggttcg tcttcggggc
     5401 gatcctcctc ggcatcgtca tgggcgtgat gcgcctacgg ctgccgcggc cgtgggactc
     5461 tgctggggcc gcgctctacg gcctcctcgg cttcggcgga gcgtacgcgc tggcctacgt
     5521 cgcgctcgtc cgcctccacg cggggatcgg gcagacgctg ctggcgctgg tcccgctggc
     5581 cacactcctc ctggccgtcg cgtggcggca ggaacgcctg cgtcgggagg gcgtgatcgg
     5641 cggcgtggtg gcgctcacgg gcgtcgccgt catctccggc ggctccgtcg acgcgaccct
     5701 cccgctcccg tcggtgctcg ccgcgctcgg gagcgtgctg tgcatcgcgc agtccgcggt
     5761 cctcgtgcga cgactcccgc cgatgcaccc ggtcagcatg aacaccctcg gcatggccgc
     5821 tggcgcgctc ctgctggtcc ttctctccgc catcgtaggg gaacctcatc cgctgcccac
     5881 ccaagcggaa acgtggctcg ctctcgctta cgtcgttccg atcgggtcgg tcgtcgtctt
     5941 catcctgtac ctgatggtgc tgaggcaatg ggcggcctcg cgtgccgcgt acgggttcgt
     6001 catcatcccc ttcgtcacgg tcgcgctctc ggcatggctc gacgacgagc ccctccggtg
     6061 gtcgctcatc ttcggcgggc cactcgttct cgtcggggtc tacctggggg ccctgcgcac
     6121 gcgctcattc gccccaaccg aagcggcgcc cggatacgac gagcgatccg cgcggtgacg
     6181 gctcaggcgt cggagatcgt gccggtcgcg acgccgacct ggatcgtcgc ctcggtggcc
     6241 ccggacagcc gcacctcgaa ccactcctct ccctccgtga ggtcatcctc gaggatgggc
     6301 acgaagatcg tctgcgtgct gtcgtcgggc tcgaacgtga gcgttcccga gaccggccag
     6361 tagtcggacc tggcggtcgc gctcccgtcc accgtcgtga agttcacccg cacggtgcgg
     6421 gcggtgacgc cggtccggcg gacggtgaag gccatcgcgc ccaccgtctc ggttccggac
     6481 gtgcccgaga ccgcaagcga tgactccggc ggcaggaccg accggagcca cgagtagatc
     6541 ggatcctcac cgacgctcgc atagctaccg tagaagagag ggaacccgca gccgagcccc
     6601 ttccacgtcg tgccgacgac gatccagcgg ccgcggctat ccggcaccat cagcggcccg
     6661 ccggagtcgc cctggcacgc gtccttcccg ccgatggtct cgccggcgca gatctcgtag
     6721 gcgccgttga agccgaccgt cgggccgttg agccgggcgc agtcgacgtc ggagacgacc
     6781 ggcacgtcga cctcgtggag ggtgtccggc gatggaccga cgagatagaa gctcgtcccc
     6841 cagccgatcg tgcgagccgt cgttccgggc ggccagaggg tggcggcctc ctccggcatg
     6901 ccgatccgga tcggggtctg cgtcgacggc gtcgtcagcc gcaggacggc ggagtcgctc
     6961 gcggagggcg cgtgggtgat cacctgcgcg atctggtgga tctgcccacc ctgcgacttc
     7021 gaggtcgatc cgatccggac ccggaacgcg ctcggggcgc gcccggcgac gcagtgcttc
     7081 gcggtcagga cccactccgg gcgaacgagg ctgccgccgc agaagtgggt gccggagttg
     7141 ttctggagcg agaccatgaa cgggtacggc tgggtcgccg ggcggccgcc gacgatctgc
     7201 tgacgggcgt tcgccgggaa gacagggagc gcgaccagga cgagcacgag catggcggtg
     7261 cggcgcatgg atcctcctgt tgtcgggtgc tctcgtcgcg tcaacttcca cgcgcgaggc
     7321 cgggcctcct ttgtgcacct gcacagagat ggcggcctcc ccgccgccgc ggggcgggcc
     7381 cggaccgagc aggagagcgc cccacgggcg gcgaatagca gaggtatgag gcctcgcgca
     7441 tcacgagtcg ccctcgccac gaccctcatg gccagcctcg tgctgcccgg aagcggcctc
     7501 gctgcggcga gcgatgagcg ctgcaccgat cggcgggacg ccgctctctc aacccgacct
     7561 cgcctgcggc tgtccggcct ctacgcgttg ccccggaagc cggctcggac gctcgtggtg
     7621 ttcgcgcacg ggtaccgcaa gccggcgacg gtctactggc aacggcacct gcgccgagag
     7681 gcccgcaggg gcgcgatcgc ggtcgcgatg gactaccgcg ggatcggcgg cgcgccggac
     7741 taccgcggtt ggaacgtgcg agcgggtgcg gccgattcga tccgggccgc gaggcacttc
     7801 tggcagggat gccgctcgct gcgacggatc atcctcgtga gcgtctcgat gggcagcaac
     7861 gccgccgggc tcgcactggc ggcccagccg gttcgccatt cgtcgccatg taccgaccgc
     7921 gctgggcaac cgagatgcgc cgcaccgtat ttcccggagc cgctgttcga ccactgggtg
     7981 aacgtcgagg gggcggcgaa cgtcacggag acgtatctgg aggccgtcgc cgtcgcgccg
     8041 gggaacgcgt atgcccgcgc tgcgaaggag gacatcgagc gcgagatggg agggaccatc
     8101 gccgaggtgc ccgagcggta ccgtgaggcg acgctcgttg cgcgcgccga ggacatcgca
     8161 gcgtcggggg tgcggagcgt gaccttcgtc cacgggctcg aggacgggct cgtcccctac
     8221 gaccagagcc gtgagctcgc ggcggcgctc cgtcgccacg acgtgccctc cgacttcttc
     8281 accgtcctga cgcggggcga gggcgacgac ggcacaacca tcagcgggta cgcgctcggc
     8341 cccgccggtc agaactcccc gttcgcgggc cacgggtccg aggaggacat cacgcacctc
     8401 gtgatccgga ccgggttcga gcgactctgg gcgatccgtc ggggcgcgac ggtcggcccc
     8461 gccagggagt tcgtcgtcga cgggatcctc ggcacgcatc ccgcgccctg aacccgctcc
     8521 gacggggtct tccgggtccg ccgcttcgtc atctttgggc aggtgcacaa aggaagccgc
     8581 gggggtcgtg acgaaccgtt gggcgcacac gaccgaggag gagacatgct cgccaagctc
     8641 gccatcgtcg ccctcgcatt ccttctggcc gttcccgcgg ccgcccagac cccgggtgcg
     8701 ctcccgtcgc tcgccgaggt ggagctcgac cgcaccgccg tcgcggccgg gcagccgatc
     8761 cgggtcacgg gccggctcct gacgcaggtg cagcccccgg cgcgtctgtg ggtggacgcc
     8821 acgcttcccg gggatctcgg ctcgttcaac tatccggtcg atccgcagct ggacctcgtc
     8881 gaggggtggg tggcgcgcga gcggcccggc gacccgctga cgatgcagat caaggtggct
     8941 ccggcgatcg gcgccgactc cgggccgaag ccctacatgc ggttccgctg gtccttccac
     9001 ctgaacgacc tgtggtactt cgtccgcttc cgctacctct ccgacgggac gggcaccggg
     9061 ttctcctggc agggcaactg gtactactgc cggctcgcgc tcggctccaa ctgcttccac
     9121 ggggacacgc cccccttccc cgtgacgtgg gacgcggcga cgcgcacgtt cacgggccgg
     9181 atccggctcg ccgatctcgc ggcgaagcag gagagcggga ggctcgtgca ggacacgatg
     9241 cgcccgttct cgagccagta ccccccggag tccggctacg agctgcccgt gatcacgatc
     9301 ggccaggact cgtccgcgcc gttcgcgcct tacgacctgc cgatcgaaac cgtgttcctg
     9361 gggatcgctc ccgtcgggtc cgcaccgcag agcgtgaact actcggtcgc cgccgacgtt
     9421 cagacgacgg ccggctcgct cgacgtgccg ttctcggggc agatctcaac ggaagggctc
     9481 gccacgggcg cttacgacct ttgggtgaag ggctgcttcg gcccctgcgt cacgaggacc
     9541 ttcaggatca gcgtcagctg aggccggggg taacggaagg gcgccgggga agcccggcgc
     9601 ccttcgagtg ttcggaacga tcgtggttac ggggtgggga tggttcccgg cgtaggaagg
     9661 ccctcgggac ggctccccga cccgccggac ccagaacccc cggacccgga gccgccgccc
     9721 gatccggagc cgccggaccc gccaccgctg ccgccggccg tgccgttgcc accgccaccg
     9781 gagccgccgc ccgatccgga gc
//
LOCUS       DATLGG010000135         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_25340, whole genome shotgun sequence.
ACCESSION   DATLGG010000135 DATLGG010000000
VERSION     DATLGG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25340"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="gatB"
                     /locus_tag="VM638_03875"
     CDS             <1..1078
                     /gene="gatB"
                     /locus_tag="VM638_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVM11602.1"
                     /translation="LEEDTGKTLHAGTTGRIAEAEYALEDFNRAGVPLVEVVSEPDIR
                     SPEEAGAYLRELRELLRSLGVSDVRMEEGSLRCDANISLRRVGEDAYGTKVEIKNMNS
                     IRSVERALRYEAERQRAALDAGEPIVQETRHWDEDRGTTSSLRSKEEAFDYRYFPDPD
                     LVPLEPDDEWIERLRAELPELPAARRQRFERDYGLDPSVARLVGATEWAPFFEESVRL
                     GADPRTAANWMTGDLAGLLNEARVELDESKVTPQHLAELSALVADGTISTAGAKQVLA
                     EAFASGAGARAIVDEKGLAQISDEGALGAIVDEVIAENPGPVEQFRGGKEGVIGFLVG
                     AVMKKSRGAANPQEAQRLLRDRLS"
     gene            complement(1071..1325)
                     /locus_tag="VM638_03880"
     CDS             complement(1071..1325)
                     /locus_tag="VM638_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HVM11603.1"
                     /translation="MTTTTACPHVTDTAAAPSGDRCEECGSKVNLRVCTECGHVGCCE
                     SQQGHNTAHARAAGHPIIRSLPLGPGSFTWCYECDRYVAS"
     gene            complement(1346..2149)
                     /locus_tag="VM638_03885"
     CDS             complement(1346..2149)
                     /locus_tag="VM638_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVM11604.1"
                     /translation="MPFRRIVVGTDGSDTAAVAVAQTVGLAERLDAEVVVVTAYGKGE
                     GGDDTPLAVANGLLRDVRRLHPSARIETIAREGKDPAEVIVGAAAETSADLVVVGNRG
                     MTGRRVLGGIPDRVSHHAPCSVLVADTAWAAILPSDEDPHARREVGSLLVATDGSPTA
                     EVAVDTGVGLAEALGAEVVLLFVGEREHGEKVLGRTRERIGERSGVRPVVVAGDPPSR
                     VLEVAAAEAVDLIVVGSKGMRGMRRVFLGGVPNHVTHHADRSVLVVKTS"
     gene            2255..>3402
                     /locus_tag="VM638_03890"
     CDS             2255..>3402
                     /locus_tag="VM638_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HVM11605.1"
                     /translation="MSETIRFDLTQDELPSSWYNIMPAIAGAGIQPLPPLHPGTHEPV
                     TPDLLAPLFPESLIMQEVSTDQWIDIPGPIMDVYRLWRPTPLIRATRLEQALGTPARI
                     YYKYEGTSPSGSHKSNTAVAQAYYNKEAGTRQIATETGAGQWGSALSMACRFFDIECL
                     VFMVKASYEQKPYRRVFMETFRAEVRPSPSSTTEAGKKILDADPESTGSLGIAISEAI
                     EVAAQNDDSKYSLGSVLNHVLLHQTVIGLEAQAQMEKAGDTPDVVIGCVGGGSNYAGL
                     AYPFMGERLGGGAQTRFVAAEPAACPTLTKGEFRFDFGDTGQMTPLLPMYTLGHTFKP
                     APVHAGGLRYHGDAPSLSLLVKEGHMEARAFTQNEIFEAATTFAHTQG"
BASE COUNT          521 a         1206 c         1207 g          468 t
ORIGIN      
        1 cctcgaggag gacactggca agacgctgca cgcgggcacg accggccgca tcgcggaagc
       61 cgagtatgcg ctcgaggact tcaaccgcgc gggcgtgccg ctcgtcgagg tcgtctccga
      121 acccgacatc cggtccccgg aggaggccgg cgcctacctg cgcgagctgc gtgagctcct
      181 gcgctcgctc ggcgtctccg acgttcgcat ggaggaaggc tcgctgcgct gcgacgcgaa
      241 catctcgctg cgccgtgtcg gcgaggacgc ttacggcacg aaggtcgaga tcaagaacat
      301 gaactcgatc cgatcggtcg agcgcgcgct ccggtacgag gcggagcgtc agcgggccgc
      361 gctcgacgcg ggggagccga tcgtgcagga gacgcgccac tgggacgagg atcgcggcac
      421 gacctcatcg ctccgctcga aggaggaggc gttcgactac cggtacttcc ccgacccgga
      481 cctcgtcccc ctcgagccgg acgacgagtg gatcgagcgg ctgcgcgcgg agctgccgga
      541 gctgccggcg gcgcggcggc agcgcttcga gcgcgactac gggctcgacc cgtcggtcgc
      601 gcggctcgtc ggcgcgaccg agtgggcgcc gttcttcgag gagtcggtgc gcctcggtgc
      661 cgaccccagg acggccgcga actggatgac cggcgacctt gccgggctgc tgaacgaggc
      721 acgggtggaa ctagatgagt cgaaggtgac gccccagcac ctcgccgaac tctcggcgct
      781 cgtcgccgac ggcacgatct cgacggccgg cgcgaagcag gtgctcgccg aggccttcgc
      841 ctccggagcc ggggcgcgag cgatcgtcga cgagaagggc ctcgcgcaga tctccgacga
      901 gggcgcgctc ggcgcgatcg tcgacgaggt gatcgccgag aacccgggtc cggtcgagca
      961 gttccgcggc ggcaaggagg gcgtgatcgg cttcctcgtg ggggccgtca tgaagaagtc
     1021 gcgcggggcc gccaaccccc aggaggccca gcgcctcctc cgcgatcgcc tcagctagcg
     1081 acgtaccggt cgcactcgta gcaccaggtg aacgaccccg ggccgagcgg cagggacctg
     1141 atgatcgggt gaccggccgc gcgcgcatgg gcggtgttgt gcccctgctg ggactcgcag
     1201 cagccgacgt ggccgcactc ggtgcagacg cggaggttca ccttgctccc gcactcctcg
     1261 cagcggtcgc cggagggcgc ggcggccgta tcggtgacgt gcgggcaagc ggtcgtggtc
     1321 gtcatgcctc gatggtaccc gcgtctcagc tcgtcttcac gacgaggacg ctacggtcgg
     1381 cgtggtgggt gacgtggttc gggacgcccc cgaggaagac gcggcgcatc ccgcgcatcc
     1441 ccttgctccc gacgacgatc aggtcgaccg cttccgcagc ggccacctcg aggacgcggc
     1501 tcggcgggtc gcccgcaacg accaccggcc ggaccccgga acgctcgccg atccgttccc
     1561 gcgtgcggcc cagcaccttc tcgccgtgct cgcgctcccc gacgaacagg aggaccacct
     1621 ccgcgccgag cgcctcggca agaccgacac ccgtgtcgac cgccacctcc gcggtcggag
     1681 agccgtcggt cgcgacgagc aggcttccga cctcacggcg agcatgcggg tcctcgtcgc
     1741 tcgggaggat cgcggcccag gcggtgtccg cgacgaggac gctgcacggc gcgtggtgcg
     1801 agacgcggtc ggggatcccg ccgagcaccc gccgcccggt catcccgcgg ttccccacga
     1861 cgacgaggtc cgcggaggtc tccgcggccg ccccgacgat gacctcggcc gggtctttcc
     1921 cctcgcgcgc gatcgtttcg atgcgcgcgc tggggtggag ccggcgaacg tcgcgcagga
     1981 ggccgttcgc caccgcgagc ggcgtgtcgt cgccaccctc gcccttgccg taggcggtga
     2041 cgaccacgac ctcggcgtcc aaacgctccg ccaggccaac cgtctgcgcg accgcgaccg
     2101 cggccgtgtc ggacccgtcg gtgcccacga cgatccttcg gaacggcatc gggtcatctt
     2161 cgcccacgcg cggaagacgc gcgagcgccc gtggcttgac cggatccggt ggccgggggg
     2221 agactcgagc ctcccccatc gaggaggcgt ggccatgagc gagacgatcc ggttcgacct
     2281 cacgcaggac gagcttccga gctcctggta caacatcatg ccggcgatcg ccggggcggg
     2341 gatccagccg ctgccccccc tccacccggg gacgcacgag ccggtgaccc cggatctcct
     2401 ggcgccgctc ttcccggagt ccctgatcat gcaggaggtc tcgaccgatc agtggatcga
     2461 catcccgggt ccgatcatgg acgtgtaccg cctgtggagg cccacgccgc tcatccgggc
     2521 aacgcgactc gagcaggcgc tcgggacgcc ggctcgcatc tactacaaat acgaggggac
     2581 ctcgccgtcc ggctcgcaca agtcgaacac cgcggtcgcg caggcctact acaacaagga
     2641 agccgggacg cggcagatcg cgaccgagac cggcgccggt cagtgggggt cggccctctc
     2701 gatggcctgt cgcttcttcg acatcgagtg cctggtcttc atggtgaagg cctcctacga
     2761 gcagaagccg taccggcgcg tgttcatgga gacgttccgc gccgaggtgc ggccctcgcc
     2821 gtcatcgacg accgaggcgg ggaagaagat cctcgacgcg gacccggagt cgacggggtc
     2881 gctcgggatc gcgatcagcg aagcgatcga ggtcgcggcg cagaacgacg actcgaagta
     2941 ctcgctcggc tcggtcctga accacgtgct gctccaccag accgtgatcg gtctcgaggc
     3001 gcaggcgcag atggagaagg cgggcgacac gcccgacgtc gtgatcggct gcgtcggcgg
     3061 cggatcgaac tacgccggcc tcgcgtaccc gttcatgggc gagaggctgg gtggcggggc
     3121 gcagacgcgg ttcgtcgcgg ccgagcccgc ggcctgcccg acgctgacga agggcgagtt
     3181 ccgcttcgac ttcggcgaca cgggtcagat gacgccactc ctgccgatgt acacgctcgg
     3241 ccacaccttc aagccggccc cggtgcacgc ggggggcctt cggtaccacg gcgatgcgcc
     3301 ctcgctctcc ctcctggtga aggaggggca catggaggcg cgggcgttca cgcagaacga
     3361 gatcttcgag gcggccacga ccttcgccca cacccagggg at
//
LOCUS       DATLGG010000136         4177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_25353, whole genome shotgun sequence.
ACCESSION   DATLGG010000136 DATLGG010000000
VERSION     DATLGG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25353"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VM638_03895"
     CDS             <1..345
                     /locus_tag="VM638_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVM11606.1"
                     /translation="VVSPLLEVPRLPYPGQMLYLFGHSILPTIPASFLTFGDAPLYPV
                     YAELPRVFGISVMADQMVAGLIMKIGGGLLLWSIIAILFFKWHTQESTEGIDALQWRD
                     VGRQLDRVEVTP"
     gene            342..1046
                     /locus_tag="VM638_03900"
     CDS             342..1046
                     /locus_tag="VM638_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVM11607.1"
                     /translation="MSRESRDRLLLPALLPILAILIIAILVIAMSRVLLAVPHEMATP
                     IALGLALNVLTGAALIASWPSLRGKAVVAMLVVVGLVVVGGGVTAMAVSGELHELREA
                     LARDEVAPEGGLATGEQPGPGETGEQGAPGETGEPPPPVTEPTTDVVAQQMAFDVDEL
                     LFVAGEATTLTFRNEDAAPHNIAIYTEPGAETLFSGEVFSGPDEREYEIPAMEPGRYY
                     FHCDVHPPMNGAVTVG"
     gene            complement(1033..1434)
                     /locus_tag="VM638_03905"
     CDS             complement(1033..1434)
                     /locus_tag="VM638_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11608.1"
                     /translation="MARREPALAAALCSAGSLLGLVPHLMEDLVSGIPERFGISHLVF
                     EWSMGVAVASTVVAAVLAVRGRRRALIAAAVIGVGWVLAAVLDHPGAFTPDAFREGLS
                     SRLWVWIVTIGQGAAAVFAIAALRRSGATRR"
     gene            complement(1438..2370)
                     /gene="ccsB"
                     /locus_tag="VM638_03910"
     CDS             complement(1438..2370)
                     /gene="ccsB"
                     /locus_tag="VM638_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HVM11609.1"
                     /translation="MTRASNFAFGLALALYLVGALAYFHHLAFRRRGSWIAGTSLATL
                     GAAVHLVSIVLRGVAAGRVPWGNMYEYASVVGFGVVVGYLVLERVSRVRAVGGFAIGA
                     SVLAMAGATMLYVPPGPLVPALNSYWIRIHVLAAIVGSSLFILGAVFTILYLVRDAGE
                     RRAMARQRDEEREPILAGVGGGGLPDDYAVGADRSTETVEPSRGRLPASDILDRLAYR
                     TIAFGFPVWTFAVIAGAIWAEQAWGRYWGWDPKEVWSFVTWVVFAAYLHARATVGWRG
                     RRAAVLALVGFGALVFNLVVVNLVISGLHSYAGV"
     gene            complement(2367..3851)
                     /locus_tag="VM638_03915"
     CDS             complement(2367..3851)
                     /locus_tag="VM638_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HVM11610.1"
                     /translation="MPSLTRSLAAVWRALRSMRMALLLLLVVALAATAGSLVPQVQNS
                     PGAVADLFRERPLLARIYRVTGLFDVYGSWWFTAAYSLLLISLASCLGPRTRAMVRAL
                     RRRPQPQRELEGMRHVAAARLPEEPGEALERARRLLRRRRFRVATAEGMLSAEKGLAR
                     EAGSLMFHWSFFLLLLGAVVGKGFGFTGQATIVEGDTFTESPASYDTPPVHGRFFSER
                     MHRGFQVRLRDFDVSYRETGLPREFVSRVDVLEDGRLVRREEIRVNHPLHHRGVKLFQ
                     QGYGWAPSVSVRHEGRTLAQGPVVFVVSSEQDRREPWRGVVKLPSLRPQVGIEFRLLP
                     DPVAALAGRPMLEAREPFLAYTVYEGDLGLTRAQSVFRLDTERMREIGSGGIGLGGRT
                     EGPRGLEISFDELREYTQLRITRDPGTGIMLAAAILILLGLIPALYSSRRRIWVQADP
                     EGGGTRLTVGGFALQRRAAFEEEFASLVAALLGERDPSGRVETG"
     gene            complement(3873..>4177)
                     /locus_tag="VM638_03920"
     CDS             complement(3873..>4177)
                     /locus_tag="VM638_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HVM11611.1"
                     /translation="IGPVLAGILTLAAAQGGGVWGAVLMFSYSLGLGLPFILVGLGVR
                     RLVGVLAAVKRNYHWFAGVGGALLVTIGVLLALNLWVPLLSRLGLLRLVRSFTPPL"
BASE COUNT          676 a         1553 c         1471 g          477 t
ORIGIN      
        1 gtcgtgagcc ccctcctcga ggtgccgcgg ctgccctacc ccggccagat gctctacctg
       61 ttcgggcact cgatcctgcc cacgatcccg gcctcgttcc tcaccttcgg cgacgcgccc
      121 ctctacccgg tctacgcgga gctcccgcgc gtgttcggga tctcggtgat ggcggaccag
      181 atggtcgccg ggctgatcat gaagatcggc ggcgggctcc tgctgtggag catcatcgcg
      241 atcctgttct tcaagtggca cacgcaggag agcacggagg ggatcgacgc actccagtgg
      301 cgcgacgtcg gccgccagct cgaccgggtg gaggtgacgc cgtgagccgc gaatcacgcg
      361 accggctgct gctccccgcg cttctcccga tcctggcgat cctgatcatc gccatcctcg
      421 tgatcgcgat gtcccgcgtg ctcctcgcgg tcccgcacga gatggcgacg ccgatcgcgc
      481 tcgggctcgc gttgaacgtg ctgacgggcg cggcgctcat cgcgtcgtgg ccctcgctcc
      541 gcggcaaggc ggtggtcgcg atgctcgtcg tcgtcgggct cgtggtcgtc ggcggcggcg
      601 tgacggcgat ggcggtcagc ggggagctgc acgagctgcg agaggcgctg gcccgcgatg
      661 aggtcgcacc tgaaggtggc ctggcgaccg gggaacagcc gggccccggg gagaccggtg
      721 agcagggcgc gcccggcgag accggcgagc ccccgccgcc ggtgacggag ccgaccacgg
      781 atgtcgtcgc gcagcagatg gccttcgacg tcgacgagct cctcttcgtc gccggcgagg
      841 cgacgaccct cacgttccgc aacgaggacg ccgcgccgca caacatcgcg atctacaccg
      901 agcccggcgc cgagacgctg ttcagcggcg aggtgttctc cgggcccgac gagcgggagt
      961 acgagatccc cgcgatggag cccgggcgct actacttcca ctgcgacgtc cacccgccga
     1021 tgaacggcgc ggtcaccgtc gggtagcgcc gctcctccgc aacgccgcga tcgcgaacac
     1081 cgccgccgcg ccctgtccga tcgtcacgat ccacacccaa agccgggagg agagcccctc
     1141 gcggaaggcg tcgggggtga aggcgccggg gtggtcgagc acggccgcca ggacccagcc
     1201 cacgccgatc accgccgccg cgatcagcgc acgtcgccgg ccccggacgg cgaggaccgc
     1261 ggcaaccacc gtggatgcga ccgccacgcc catcgaccac tcgaacacga gatgggagat
     1321 gccgaaccgt tcggggatgc cggagacgag gtcctccatc aggtgcggga cgagcccgag
     1381 caggctcccc gcggagcaga gcgcggccgc gagcgccggt tcgcgtcgcg ccacccgcta
     1441 gacccccgcg taggagtgga gacccgagat gacgaggttc acgacgacga ggttgaacac
     1501 gagcgcgccg aacccgacca gcgcgaggac ggccgcccgg cggccgcgcc atccgacggt
     1561 cgcacgcgcg tgcaggtacg cggcgaagac cacccaggtg acgaaggacc agacctcctt
     1621 cgggtcccac ccccagtacc gaccccaggc ctgctccgcc cagatcgccc ccgcgatcac
     1681 ggcgaaggtc cagacgggga agccgaacgc gatcgtgcgg taggcgaggc ggtcgaggat
     1741 gtccgaggcg ggcaggcggc ctcgcgacgg ctccaccgtc tcggtggacc ggtccgcccc
     1801 gacggcgtag tcgtccggca ggccgccacc gccgacgccg gccaggatgg gttcgcgctc
     1861 ctcgtcccgc tggcgcgcca tcgcccgccg ctcccccgcg tcgcgcacga ggtagaggat
     1921 cgtgaacacc gcgccgagga tgaacagcga cgaccccacg atcgccgcga gcacgtggat
     1981 ccggatccag tacgagttga gcgccgggac gaggggcccc ggcgggacgt agagcatcgt
     2041 cgcaccggcc atcgccagga ccgacgcgcc gatcgcgaac ccgccgaccg cgcggacccg
     2101 cgacacccgc tcgagcacga ggtagccgac gacgaccccg aacccgacca ccgacgcgta
     2161 ctcgtacatg ttcccccagg ggacgcgtcc ggccgcgacc ccgcgcagca cgatcgagac
     2221 cagatgcacc gcggcgccca gcgtggccag cgacgttccc gcgatccacg acccccggcg
     2281 ccggaaggcg aggtggtgga agtacgcgag cgcgcccacg aggtacagcg cgagggcgag
     2341 gccgaacgcg aagttcgagg cgcgcgtcat cccgtctcga ctctaccgga tgggtctcgc
     2401 tcgccgagca gcgccgcgac gagcgacgcg aactcctcct cgaacgcggc acgccgttgg
     2461 agcgcgaacc cgccgaccgt gagccgcgtg ccgccgccct ccggatccgc ctgcacccag
     2521 atcctccggc gggacgagta gagggcgggg atgaggccga gcaggatcag gatggccgcc
     2581 gcgagcatga tccccgtacc ggggtcacgc gtgatgcgga gctgcgtgta ctcccgaagc
     2641 tcgtcgaagg agatctcgag cccgcgaggc ccttcggtcc tcccgccgag cccgatcccc
     2701 cccgagccga tctcgcgcat gcgctccgtg tcgagccgga agacgctctg cgctcgcgtc
     2761 agcccgaggt cgccctcgta gaccgtgtag gcgaggaagg gctcccgcgc ctcgagcatc
     2821 gggcggcccg cgagcgccgc gacgggatcc ggcagcagcc ggaactcgat ccccacctgc
     2881 ggccgcagcg acgggagctt caccaccccc cgccagggct cccggcggtc ctgctccgat
     2941 gagacgacga agacgaccgg gccctgcgcg agcgtcctcc cctcgtggcg gaccgagacc
     3001 gacggcgccc agccgtagcc ctgctggaag agcttcacgc cgcggtggtg cagggggtgg
     3061 ttcacccgga tctcctcccg ccgcacgagc cgcccgtcct cgaggacgtc gacgcggctg
     3121 acgaactccc tcggcaggcc ggtctcccgg tacgagacgt cgaagtcccg cagccgcacc
     3181 tggaacccgc ggtgcatccg ttcggagaag aagcgcccgt ggaccggcgg cgtgtcgtag
     3241 ctcgccgggg actcggtgaa cgtgtccccc tcgacgatcg tcgcctggcc cgtgaagccg
     3301 aaccccttgc cgaccaccgc cccgaggagg agcaggaaga acgaccagtg gaacatcaag
     3361 ctcccggcct cgcgtgcgag acccttctcg gccgagagca tcccctcggc ggtggcgacg
     3421 cggaaccggc ggcggcgcag gagccgtcgc gcacgctcga gcgcttcccc gggttcctcc
     3481 ggcagccggg cggcggcgac gtggcgcatc ccctcgagct cccgctgtgg ctgtggccgc
     3541 cgacgcagcg cgcggaccat cgcccgtgtg cggggaccga ggcaggaggc gagcgagatc
     3601 agcagcagcg agtacgccgc ggtgaaccac cacgatccgt agacgtcgaa gaggccggtc
     3661 acgcggtaga tccgcgcgag cagcggccgt tcgcggaaca ggtccgcgac cgcgccgggc
     3721 gagttctgca cctgcggcac gagcgacccc gccgtcgccg ccagcgcgac gacgagcagc
     3781 aggagcagcg ccatgcgcat cgaccgcagc gcgcgccaga cggccgcgag ggaccgggtg
     3841 agtgacggca tcgcggcgag tctagccagg ggctacagcg gcggcgtgaa gctgcgcacc
     3901 aggcgcagca ggccgagccg cgacaggagc ggcacccaca ggttgagcgc gagcaggacc
     3961 ccgatcgtca cgaggagggc gccgccgaca ccggcgaacc agtggtagtt ccgcttcacg
     4021 gcggcgagga ccccgacgag gcgccggacg ccgagcccga ccaggatgaa cggcagcccg
     4081 agcccgaggg agtacgagaa catcaggacg gcgccccaga cgccgcctcc ctgcgccgcc
     4141 gccagcgtga ggatcccggc gagcacgggc ccgatgc
//
LOCUS       DATLGG010000137         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_4600, whole genome shotgun sequence.
ACCESSION   DATLGG010000137 DATLGG010000000
VERSION     DATLGG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4600"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VM638_03925"
     CDS             complement(<1..104)
                     /locus_tag="VM638_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HVM11612.1"
                     /translation="MRVIAGSARGMRLAGPRGSTRPVSDRAREGLFASL"
     gene            complement(106..2253)
                     /gene="recG"
                     /locus_tag="VM638_03930"
     CDS             complement(106..2253)
                     /gene="recG"
                     /locus_tag="VM638_03930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVM11613.1"
                     /translation="MAALSLGSSIETLAAGWARRYVGALRDLGVETVGDLLHHYPRRY
                     IDRSDVAKIGEVRLNQEPTVIGTVRKVHMAQTRHRKAMVTVSVWDGTGSLDLFWFNQR
                     WPMKRFRQGQEVAASGKVSAFRGRLRIQNPAVEVLRGGDADQVHTGRIVPVHGAGEGV
                     TTGVIRRLVHDALQRVPPIPDPLPPEVVERQSLLPLERALHDVHFPEDDADRQRALER
                     LKFDELFFLGLGVAFRKRRVERSTPGVAHDLDAKLAVRFLEALPFPLTDAQRRATGEI
                     ARDMAAGKPMNRLLQGDVGSGKTVVALYACLVAVGSGHQAAIMAPTEVLAQQHARSVA
                     ALLGPLGGRRLGVTGGGAQPDLFATEERGPVHALLTASVTGKERERVYEAVAAGEVDV
                     LIGTHALVQEGVAFRNLSLAVVDEQHRFGLHQRIALKEKGVQPDVLIMTATPIPRTLA
                     LTYYGDLDVSVLDEMPAGRQPIETRAARTPAERRRAYDLVREQVREGRQAFVVTAAID
                     EGNRLEVKAAESEAARLAAEVFPELEVELLHGRMRPKEKDRVMERFRTGEADILISTT
                     VIEVGVDVPNASVMLIENAERFGLAQLHQLRGRIGRGAHRSYCILFDESPPDNEEARA
                     RIEAMVATTDGFRLADEDLRLRGEGTLFDIRQSGMPDLRLARLAHDTDLVRRSRREAF
                     DLIDEDAELERHAELRVEMERRFADSIDWLFHS"
BASE COUNT          316 a          879 c          837 g          337 t
ORIGIN      
        1 agactggcga acaacccctc gcgggcgcgg tcggagaccg gtcgcgtcga gccgcgcggt
       61 cccgcgagcc tcatcccgcg cgccgacccg gcgatcaccc gcatctcagg agtggaagag
      121 ccagtcgatc gagtctgcga agcggcgctc catctccacg cgcagctccg cgtgccgctc
      181 gagctccgca tcctcgtcga tcaggtcgaa ggcctcgcgc cgggagcggc ggacgagatc
      241 ggtgtcgtgg gcgagccgcg cgagccgcag gtcgggcatc cccgactgcc ggatgtcgaa
      301 cagcgtcccc tccccgcgca gccgcaggtc ctcgtcggcg agccggaacc cgtcggtggt
      361 cgcgaccatc gcctcgatcc gggcgcgcgc ctcctcgttg tcgggcggtg attcgtcgaa
      421 caggatgcag tacgagcggt gcgctccgcg gccgatgcgg cctcgcagct ggtggagctg
      481 ggcgagcccg aagcgctccg cgttctcgat cagcatcacg ctcgcgttcg gcacgtcgac
      541 gccgacctcg atcacggtcg tcgagatcag gatgtccgcc tcgccggtgc ggaagcgctc
      601 catcacgcgg tccttctcct tcgggcgcat ccgcccgtgc aggagctcga cctcgagctc
      661 ggggaagacc tcggcggcga gccgcgcggc ctccgactcc gccgccttga cctcgagccg
      721 gttcccctcg tcgatcgcgg cggtcaccac gaacgcctgc cgcccctcgc ggacctgttc
      781 gcggacgagg tcgtaggccc ggcgccgctc cgcgggcgtg cgcgcggcgc gggtctcgat
      841 cggctggcgc cctgcgggca tctcgtcgag caccgacacg tcgaggtcgc cgtagtacgt
      901 gagggcgagc gtccgcggga tcggcgtcgc cgtcatgatc aggacgtccg gctgcacccc
      961 cttctccttc aacgcgatcc gctgatggag cccgaagcgg tgctgctcgt cgacgacggc
     1021 gagcgacagg ttgcggaacg ccaccccctc ctgcacgagc gcgtgggtcc cgatcagcac
     1081 gtcgacctcc cccgccgcga ccgcctcgta cacgcgctcc cgctccttgc cggtcacgga
     1141 tgcggtgagg agcgcgtgga cgggcccccg ttcctccgtc gcgaagaggt ccggctgcgc
     1201 tcctccgccc gtgacgccga gccggcgccc gccgaggggg ccgaggagcg cggcgacgct
     1261 gcgggcgtgc tgctgggcga ggacctcggt cggggccatg atcgcggcct gatggccgga
     1321 gcccaccgcc acgagacagg cgtacagcgc cacgacggtc ttccccgacc cgacgtcgcc
     1381 ctggagcagc cggttcatcg gcttccccgc ggccatgtcg cgcgcgatct cgcccgtcgc
     1441 acgccgctga gcgtccgtca gggggaacgg cagggcctcg aggaaccgga ccgccagctt
     1501 cgcatcgagg tcgtgggcca ccccaggggt cgagcgctcc acccggcgct tgcggaacgc
     1561 gacgccgagc ccgaggaaga agagctcgtc gaacttcagg cgttccagcg cccgttggcg
     1621 gtcggcgtcg tcctcaggga agtggacgtc gtggagcgct cgctcgagcg gcaggagcga
     1681 ctggcgctcg acgacctccg gtggcagcgg gtcggggatc ggggggaccc gctggagggc
     1741 gtcgtggacg aggcgccgga tcacgccggt cgtgacccct tcgcccgccc catgcaccgg
     1801 gacgatccga cccgtgtgca cctgatccgc gtcgccgccg cgcaggacct cgacggcggg
     1861 gttctggatc cgcagcctgc cccggaacgc cgagaccttc ccggacgccg cgacctcctg
     1921 gccctggcgg aaccgcttca tcggccaccg ctggttgaac cagaacaggt cgagcgagcc
     1981 ggtcccgtcc cagacgctca ccgtcaccat cgccttgcgg tggcgcgtct gcgccatgtg
     2041 gaccttgcgg accgtgccga tcaccgtggg ctcctggttg agccggacct cgccgatctt
     2101 cgcgacgtcg gagcggtcga tgtatcggcg cgggtagtga tgcaggaggt cacccacggt
     2161 ctcgacaccg aggtcccgca gggcgccgac gtaccggcgc gcccaaccgg ccgccagggt
     2221 ctcgatggag gatccgaggg agagcgcggc caccgctcga atcctacggg acggatgccg
     2281 tccgagaccc ggtgtaccgt ggaccggtgc gccgcgcgct cgtgctgctg ctggtggctg
     2341 cggccaccgc ctgtggggga ccctccacc
//
LOCUS       DATLGG010000138        19291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_148690, whole genome shotgun sequence.
ACCESSION   DATLGG010000138 DATLGG010000000
VERSION     DATLGG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19291
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148690"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..727
                     /locus_tag="VM638_03935"
     CDS             239..727
                     /locus_tag="VM638_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVM11614.1"
                     /translation="MTRGPETMRRLYEGLDARDADAVASCYAPVATFEDPVFRLEGGD
                     VTDMWRMLIERGRDLRVEHEIVDATSARWTARYTFGGHPVTNRITSRFAFDEEGRILQ
                     QLDSFDFPRWAAQALGLKGRLFGRFRFLRSAVRRASATSLASWQRRRGRDGQASSGSQ
                     VT"
     gene            complement(1242..1316)
                     /locus_tag="VM638_03940"
     tRNA            complement(1242..1316)
                     /locus_tag="VM638_03940"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1282..1284),aa:Ala,seq:cgc)
     gene            complement(1343..2287)
                     /locus_tag="VM638_03945"
     CDS             complement(1343..2287)
                     /locus_tag="VM638_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HVM11615.1"
                     /translation="MLVDAALLVGGIALLTVAAERFVIGASRLAVLLRVSPVVVGVVI
                     IGFGTSSPEVLAAGLAALQRQPEVAVGTIVGSNIANLTLILGVAALARPIVVTSKTIE
                     REAPLSAIAVGMFAIALQGRLGRGVGLLLLLGLAWGLWMVLRAAAPDEELARETDEFV
                     RVSEPHRRGVEAIRTVLGLGGTILGAQLLLVGATGLAESLGIAGGFAGFTIVALGTSL
                     PELVTALQAARHGEPDLIVGNLLGSNLFNSLAVAATVILVRPGPLDAPTLTGIGTLVM
                     VGVAAAGWLMMATARKVVRWEAVVLIVAYLAILPLLYA"
     gene            complement(2291..2926)
                     /locus_tag="VM638_03950"
     CDS             complement(2291..2926)
                     /locus_tag="VM638_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HVM11616.1"
                     /translation="MDLTAMAAAFQRRFDAIMAAREVVLGASRRAIRAAANSIRAIHR
                     GELDEAHRLQEEARRALAEGEAAAGGHPPRVFGGMLADAQKEYAESVLTEAVIAGRDL
                     PGPEEIGVELAPYLNGMAEAIGECRRAILDMLRTGHLERSEEILRAMEDLYYLLVTMD
                     YPDGITGHLRRSTDIARGILEKTRGDLSVSIGQQRLRAALEQHADDLNRRD"
     gene            complement(2938..3402)
                     /locus_tag="VM638_03955"
     CDS             complement(2938..3402)
                     /locus_tag="VM638_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11617.1"
                     /translation="MDWLLLVALAGIWGVLLLPLPFGRRSDGNVERSLQALAYIQERQ
                     PTPGRWVLAPRRDVRFIGPGERARARLRERRRRVLSVLGEALAFSVLIGAFPPLRAMW
                     VVSALLAGLILVYLGALLRMRNRGPRTIRLPEEDLAVVRSEEPEPRRAVAAR"
     gene            complement(3499..4380)
                     /gene="galU"
                     /locus_tag="VM638_03960"
     CDS             complement(3499..4380)
                     /gene="galU"
                     /locus_tag="VM638_03960"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HVM11618.1"
                     /translation="MAKLNKAVIPAAGLGTRFLPATKAQPKEMLPLVDKPAIQYVVEE
                     AVRSGLRDLLIITGRGKRTLEDHFDRSGELENHLERAGKHDLLKQVREITEMATVHYI
                     RQRDPLGLGAAVAVAEPHAAGEPFAVLLGDDVIAAADPLLARMAEVYERYGRSVIAVQ
                     EVPREDISLYGAVKPETVEDDLVRVLDIVEKPSPEQAPSNLAVIGRYIFTPEIFDALR
                     QTAPDAGGEVQLTDAIAILAREQAVYAYVFEGARYDIGKKLDYLRATVELAAEREDLG
                     EAFRAFLADFVQRKKLL"
     gene            complement(4427..6358)
                     /locus_tag="VM638_03965"
     CDS             complement(4427..6358)
                     /locus_tag="VM638_03965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVM11619.1"
                     /translation="MTLRARLTLFFTMIVVVPLVAATVVLQVLVNREVDRRVDERLQI
                     GARAAAALWQERSEAASREVRHIASALAGALGEPGLRAQVQRARDEAGLDFVVVTSAD
                     ETILADAVGRAAYAAEVPPPRIRDLLREEEAYPMLRARVEVVSDGIRGTVTGGFYADE
                     RFARALSRGASLDIVIVHRQRTVASNGDAPARLPAGSSGPIALPDGRRALVTPIEGQP
                     SAVAVVTREAGAGGMRGLMWVIIAISLALAMLLGYALAKLLSRPLQRLAEGAMAVAGG
                     DFDTFVPAEGQGDVARLAEAFNAMTANMRRYVGELEKSRDELRRGLDRLGATLRATHD
                     LEGMLGVILDTAAVTLGARSGAVFLRSPSGRRLQLEVGHGYEATKGISLRIGEGIAGT
                     AAGGMPVLIPSGTTSAQAVPPAEPVVSTAVAVPLTRGERNMGVLALYGRTTPQPFEPD
                     DLETLGSFAGQASIAIENVLLHQEAQRLSITDGLTGVWNRRYLQLTLGKELERAQRFG
                     RPVSVLMIDIDHFKLVNDLHGHHVGDEVLIEVTRRIMSTIRSQIDSLARYGGEEFIVV
                     LPETPREGAKVVAEKILTVVRQSPFAEEAAGGFSLTVSIGVACYPEDGTVAEELIRVA
                     DIAMYRAKESGRDRLEVAD"
     gene            6463..7038
                     /locus_tag="VM638_03970"
     CDS             6463..7038
                     /locus_tag="VM638_03970"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HVM11620.1"
                     /translation="MDRAERKAELRATMRARRDEVPGEERLRRADRIAGRITALGAFR
                     AARTVLLFYSFGSEASTLPIARRVHEEGKRLLLPFLRDGTMEAAEVSPGEALTASRYG
                     PKEPSKRIAVDPREVDLVITPGLAFDRDGRRLGYGGGHYDRYLTRLRDDTLRVGLAFA
                     FQVVDEVPSEDHDERVHVVVTEDDAIEASII"
     gene            7044..7352
                     /locus_tag="VM638_03975"
     CDS             7044..7352
                     /locus_tag="VM638_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HVM11621.1"
                     /translation="MPTYEYVCLEREHRFEVFQRIGDEPVATCPECGSAVRKVFHPVG
                     VVLKGSGFYKTDSRGQKPAGKPPEGSSEKKKEPASEKKKASGSSSEGGGTKGEKKESA
                     "
     gene            7349..8152
                     /locus_tag="VM638_03980"
     CDS             7349..8152
                     /locus_tag="VM638_03980"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HVM11622.1"
                     /translation="MSEATIGVFGGSGFYSFLEGVREHDVDTPYGKPSAPPAVGEVGG
                     RRVAFIPRHGARHQYPPHEVPYRANLWAMKDLGVERIVGPCAAGSLQPRVKPGDFVIC
                     DQLVDRTSGRRDTFYDGPVTTHIGFADPYCPTMREIAIKQGREQGLDLHERGTVVVVQ
                     GPRFSTRAESKWFASAGWEVINMTQYPEAALARELELCFLNVSLITDYDVGVEGEDME
                     PVSHEAVVRVFNENNAKLRDLLFRVIPALPEERDCPCATALDGARFEVH"
     gene            8217..8846
                     /gene="cpaB"
                     /locus_tag="VM638_03985"
     CDS             8217..8846
                     /gene="cpaB"
                     /locus_tag="VM638_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVM11623.1"
                     /translation="MRRLRPRASRLLFVSSLLLGLAVTTTLKGHLARLEARAARGGPG
                     VPAVVAVSDLPRGTILGPGSIRVAEVPAVARPPGALSRLEDATGKTLAADVAAGEILT
                     GLRLRRGGPIAASLPPGLRALAIPVPVPPGSVVPGDRVDVLATSMEGAPFSETVAREV
                     EVLRVEASDVDVSAPATIFVLAAPETAERLAAATAFSQLSIAVVSSASP"
     gene            8857..9672
                     /locus_tag="VM638_03990"
     CDS             8857..9672
                     /locus_tag="VM638_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVM11624.1"
                     /translation="MVRAVVLGAVQGLTEFIPISSSGHLTIVPFLLGWEHPGLPFDVA
                     IHLGTALAVILYFHRELVALVAGTGKTLLRRGDANDRNHARIALLLFVASIPAGLAGM
                     LFEGFFEERFGEARTAGLLLFVTAGLIFGGEAVYRRRDPGVNRDLSRVGWRDAMLVGL
                     LQACAIFPGISRSGATIAAGLVTGLARDAAARFSFLLGLPAILGAGLLQVPQLPPGMD
                     TGTVVAATAVSAATGFAAIAFLVRYLRARDMRPFAWYCVLFGAVALGFWFQVS"
     gene            complement(9677..11017)
                     /locus_tag="VM638_03995"
     CDS             complement(9677..11017)
                     /locus_tag="VM638_03995"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVM11625.1"
                     /translation="MADLLDLCRAAIAGATDREEVEAYAEESRSTSIEARGGVVQKLA
                     GSESRGVGVRLVVEGRLGYAWAADPDPAEAASLLERARENARHATPDEANGLPSPADP
                     TELPGLFRGSMASLDPERKVRLALDLERVAVSTDPSVTKVEEVRYGDAISRVALASTR
                     GVAAEYARTDCWCLVAALAERDGETQSGYSFRVAREVDDLEWQEAAAEGAIRGARLLG
                     GRKPRTERIPVVLDPWAASSFLGVLSRTLSAEEVQKGRSMMAERMGETVGSEVVTLID
                     DGRLPEGPGSAPFDDEGVPTSRTPLIERGVLRAFLHNSETARRADARSTGNARRGSYR
                     GGPGVAPTNLFLEAGAEDPEALLQRAGGGVYVQDVTGVHSGANPVSGDFSVGATGLRI
                     ENGILGEPLREMTIASTLVEMLTAVRAAGNDLRFFGGSVGSPTLLLGEMTVAGT"
     gene            complement(11010..12395)
                     /locus_tag="VM638_04000"
     CDS             complement(11010..12395)
                     /locus_tag="VM638_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVM11626.1"
                     /translation="MLDESLVREILDVALSSGGSFAEVYAEERVSTSVRLDDRRVEEL
                     STGTDRGAAVRVIAGETTGYAYSNRVEPEALRRAAEAAAAAVRGSERGRVIDLRRMEP
                     PVRHPVANPAVEVPTERKVDWVREADDAARSHAPEVRQVVAGYGDSVQRLLIATSDGR
                     WVEEERNRVRLIVNVVAARDGVMQTGFEGPAGLAGVELLEQHPPSEIARKAARQAIAM
                     LDGRPAPAGEMAVVVGAAGGGVLFHEACGHGLEADTIGKEASVYRGRMGERLASGLVH
                     GVDDATVPGAWGSFSFDDEGHPAERTTLFEGGTLVGALYDGTWSRRDGTPSTGNGRRQ
                     SYASVPIPRMTNTSILPGETDPEEIIRDTKRGLYAKALGGGQVNPATGDFVFGVSEAY
                     LIEDGTITAPVRGANLIGNGIAIMQAVDAVGSDFDTRPGTCGKDGQSAPVTTGSPTLR
                     IARMTVGGTGG"
     gene            12437..14323
                     /locus_tag="VM638_04005"
     CDS             12437..14323
                     /locus_tag="VM638_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11627.1"
                     /translation="MSWRRGAGFALAAVVAPAAVYLADVAALGHRLPVGPDGPLYTWW
                     TRYAGVAGLGAIPGRPGAVALTLGLGDLFGSDASSTVAAAGPVLSIAGGLAAAATVHL
                     LLGSDLLRSGAAALFVSAWSAHLAASWIASLLLAVLFLAAVATLGEPRGSSRTVGLGA
                     ALLAAAGLTHRSMFVLIVVVVGAAAFLLLRGGSARAQAGRMLIGLGLGGGVAVGGHFL
                     LRAAPAFGDTSQDAFLRRTGLRHILADRFRERLAGDARRAALPAAAAGALWRLGRAGW
                     SRRGEVFAAVCLSWAGLTAAGMLALAFVPRAQGNRLVAFAFFVPLVAAPGFAWLWRRG
                     RQVVAVAAAAVLVGGAFLGWLRQDVYVGAHELRLAAAAGEVVRELPPGTPIVFLVDHR
                     DDAASFHVTRYWNVIRSAIPPERIADVVVSVGSPSDHRAGRPTLTGDEEHDELSRRFL
                     RPPPEPPGVTIVLRPFNRPWYAEALRDGTEVSGGVIVLGGPRTDLGAVTAGPGEGGAP
                     AWVSQAILIGGFLAGLALVGFGWTRWALRRRDRPAALWLAPSAGAAVVVLGAVGADAF
                     AVGHLRRWAIVGIAIAGYLAAWFLPRHHELGEQGVGVAGQGTDPRSTAKVADDEDQED
                     ERER"
     gene            complement(15760..16203)
                     /locus_tag="VM638_04010"
     CDS             complement(15760..16203)
                     /locus_tag="VM638_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVM11628.1"
                     /translation="MDALKLLKDDHESMKGLLEKLGSTTERGVKTREELFAKVKEELA
                     AHEAIEEEIFYPALQEHPKAKEVVLEGYEEHHVVDLISSEIDETPYDDETWGAKFSVM
                     KENIEHHIEEEEGEMFDAARQVFEKEELEELGQRMELRKKEILGR"
     gene            complement(16272..16940)
                     /locus_tag="VM638_04015"
     CDS             complement(16272..16940)
                     /locus_tag="VM638_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVM11629.1"
                     /translation="MIIRVFRGRARPGMEGAFERLLHDQAAPMFRADPRVEEARVGHP
                     TEREPREFAMLTTWRDTAGLRAYAGPDWMRPRITPPERDLLEEASVHHFHFRAEPPEG
                     LDARVRTLEAMLGLGSADGRRSAVPSDVLRVDPRRGIAFVDGVRHELPPQELALLAAL
                     AARPGEPVTAGELASVLWDDGTCVTPEDVRRVVYRLRRRIRDADRARPLIRNRRGFGY
                     VIDP"
     gene            17014..17538
                     /locus_tag="VM638_04020"
     CDS             17014..17538
                     /locus_tag="VM638_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11630.1"
                     /translation="MEKMTRGSVWLAAALILAACPAGEEPSPTPSVGPTAVSRPYPYT
                     TPTPPAEPTEIDGWYARQVGKDVAGGPGPCRRCPPYRLEVGTATLSFDRGVFRVTNDP
                     AEEGALAWKSVGHYEVEGHEVVIFNDPNCPTARGRYRWALAGGELTLEVVQDDCAFGG
                     LRWRYLTAAAWTAA"
     gene            complement(17552..18208)
                     /locus_tag="VM638_04025"
     CDS             complement(17552..18208)
                     /locus_tag="VM638_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVM11631.1"
                     /translation="MEPPPSERTKVRRLPDRGVYERDVIEAILDEALYCHVAVVVDDA
                     PRVVPTIHARDGDTLYIHGSNASRTLRALKEGSEACIVVTLLDGLVLARSGFNSSMNY
                     RSVVVYGRMREVTDPAEKERAQRVLVDHVIPGRSADVRLPTERELRQTTIMAIGLDEV
                     SAKVRSGPPKDEEDDYALPIWAGVVPVRTVAGDPEPDPKLDLAIPAPPYVRGLPRPGR
                     "
     gene            18250..18942
                     /locus_tag="VM638_04030"
     CDS             18250..18942
                     /locus_tag="VM638_04030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVM11632.1"
                     /translation="MDGSHDPGSPAVDRGDAPTILPRHTWGKDLPPVGPLTAERPEDV
                     RFLLIHHTAGSNDYRPEQVISILRGIYDVHTREKTWPDVAYNFFVDRFGGIWEGRAGS
                     RNAAIRGDSTGGHVGGSVLCCFVGHHQEEPPAEPALAAMSRLLAWLCRRHQIDPSPGA
                     TVTFVSRGSNRWPPGASLTVRTIAGHRDVSRTVCPGDQLYRMLERDLPDRVAALLPLG
                     SSGGIDPPSGAC"
     gene            18953..19216
                     /locus_tag="VM638_04035"
     CDS             18953..19216
                     /locus_tag="VM638_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11633.1"
                     /translation="MSRDELVEAYVQGRVTRRVFIRRLVAGGVSLSAAVVYSQLLGSQ
                     APAAQHEGVYPVYPPPVPPNRNRVRRQQTDPAQPVDVDPRLTG"
BASE COUNT         2688 a         7052 c         6800 g         2751 t
ORIGIN      
        1 ggctcaccac cgacgccgtc agccgctcgg cgaccgacgt caccacggcg gagtacgcgt
       61 cgagcgcctc cccctcgctc ggacgaccca tcgcggcctg ctggaacggg ccttcgagcc
      121 tcccctcgac catcgccacg acgcccacct cccttcgaga cgaactccag ggtacgaccg
      181 accgccggta ccgaacggct cccaggagat cccggccgag ggagatacga tgcggcgcgt
      241 gacccgagga cccgagacga tgcggcgtct gtacgagggt ctcgatgcgc gggacgccga
      301 tgccgtcgcc tcctgctacg cgccggtggc gaccttcgag gatcccgtct tccggctcga
      361 gggcggcgac gtcacggata tgtggcggat gctgatcgag cgggggcgtg acctgcgcgt
      421 cgagcacgag atcgtcgacg cgacgagcgc ccgctggacc gcccgctaca ccttcggcgg
      481 tcaccccgtt accaaccgca tcacgagcag gttcgcgttc gacgaagagg gccggatcct
      541 gcagcagttg gactccttcg acttcccgcg ctgggccgcc caagcgctcg gcctcaaggg
      601 tcggctcttc ggcaggttcc gcttcctgcg gtccgcggtg cggagagcca gcgctacatc
      661 cctcgcctcg tggcagagac gtcgcggacg ggatggtcaa gcctcgagcg gatcccaggt
      721 cacctagaac cgtcctcagc cagcggctgc cctggcgggc ggacccctcc agcagggtgg
      781 ccacgatggt cgagtcgcgt gctccgcctg gcgatgagac gcctgacttg cgagtgtcgc
      841 ccgcttatgt cgcccttgcg aagttgcacc cggccgtgcc aggattgttc tttggtcggc
      901 gtctaccaga gaggggtggg gggatgcgac gcgctatcgc tgcagtgttg ctgtcagccg
      961 ccgtcggggc cggaacgttc gttggagccg cctacgcgac ggacgagaac gccccgcata
     1021 gtgggcccac atcgcatcct caccacgtcc acaggggcaa tggtgagtgc gaggacatcg
     1081 acgaagtgct gttcgaacgc gaccaccgag gattgcatca gggtgctggc caaagcggcc
     1141 ccgaccgggg tccatggcac gggacctgcg cgacccacaa cgatcactag ccgcccctta
     1201 ggtcctgcgg tcccggcgct ggttcgcgca tgggctagat ctggtggagg tgaggggatt
     1261 tgaacccctg gcctctgcgt tgcgaacgca gcgctctacc cctgagctac acccccgaag
     1321 ggcatcaggg tagcagaggc gctcaggcgt acaggagggg gaggatcgcg aggtaggcga
     1381 cgatcagcac gaccgcttcc cagcggacga ccttccgagc ggtcgccatc atcagccatc
     1441 ccgccgccgc cacgccaacc atgacgagtg tgccgatccc cgtgagggtc ggcgcgtcga
     1501 ggggcccagg ccggacgagg atgaccgtgg ccgcgacggc caggctgttg aacaggttgc
     1561 tcccgaggag gttaccgacg atcagatccg gttcgccgtg gcgggccgcc tggagcgccg
     1621 tcaccagctc cgggagcgag gtgcccagcg cgacgatcgt gaaccccgcg aagccgccgg
     1681 cgatgccgag ggactcggcg aggcccgtcg cgccgacgag caagagctgg gccccgagga
     1741 tcgtcccgcc gagcccgagg acggtccgga tcgcctccac gccgcgccga tgcggctcgg
     1801 atacgcgcac gaactcgtcg gtctcccgcg ccagctcttc gtccggcgcg gcggcgcgca
     1861 ggaccatcca caggccccag gcgagcccga gcaggaggag gagaccgacc ccccggccga
     1921 gcctgccctg gagtgcgatc gcgaacatcc caacggcgat cgccgacagc ggggcctcgc
     1981 gctcgatcgt cttggaggtc acgacgatgg ggcgcgcgag cgccgcgacc ccgaggatca
     2041 gggtcaggtt cgcgatgttc gagccgacga tcgtcccgac ggcgacctcc ggctgacgct
     2101 ggagggccgc gagacccgcc gccaggacct ccggggagct cgtgccgaag ccgatgatca
     2161 cgacgccgac gaccaccggt gacacccgca ggaggacggc caggcgcgac gccccgatca
     2221 cgaaccgctc cgccgcgacc gtcaggaggg cgatcccgcc gacgagcagg gccgcatcga
     2281 ccagcatcgc tcagtcgcgg cggttcaggt cgtccgcgtg ctgctcgagc gcggcgcgga
     2341 gacgctgctg cccgatcgag acggacaggt ccccgcgcgt tttctcgagg atcccgcgcg
     2401 cgatgtcggt cgagcggcgc aggtggccgg tgatcccgtc cgggtagtcc atcgtgacga
     2461 ggaggtagta cagatcctcc atcgcgcgga ggatctcctc gctccgctcg aggtggccgg
     2521 tgcgcagcat gtcgaggatc gcccggcggc actcgccgat cgcctcggcc atcccgttga
     2581 ggtacggcgc cagctcgaca ccgatctcct cgggacccgg aagatccctt ccggcgatga
     2641 ccgcttcggt cagcacggac tcggcgtact ccttctgagc gtcggcgagc atgccgccga
     2701 agacccgcgg tggatggccg cccgcagccg cctctccctc cgcgagcgct cgccgggcct
     2761 cctcctggag ccgatgggcc tcgtcgagct caccccgatg gatcgcacgg atcgagttcg
     2821 cggccgcacg gatcgcgcgg cgagacgcgc cgagaaccac ttcacgcgcg gccatgatgg
     2881 cgtcgaaccg ccgctggaag gcggcggcca tcgctgtgag atccacgggc gcgtgactca
     2941 gcgcgcggcg acggcgcggc gcggttcggg ctcctcgctg cgcacgaccg cgaggtcctc
     3001 ctccggcagc cggatcgtcc gcggcccgcg gttccgcatc cgcagcagcg cgccgaggta
     3061 gacgaggatc aaccccgcca acagggcgct gacgacccac atcgcccgca gcggcgggaa
     3121 ggctccgatc aggacgctga acgcgagggc ctcgccgagc accgacagca cgcggcggcg
     3181 gcgctcccgc aagcgtgccc gagcacgctc gcccggtccg atgaaacgca cgtcgcgacg
     3241 aggcgcgagc acccagcgtc cgggggtcgg ctgacgttcc tggatgtatg ccaaggcctg
     3301 aaggctcctt tcgacgttcc cgtcgctcct cctgccgaac ggcaggggga ggagcagaac
     3361 gccccagatc ccggccaatg ctacaagcag cagccagtcc accaccaagc cctccacggc
     3421 cggccccggc ccgaccgcaa gatgctgggc cgctccgaac gtaacagccc agatgtgggg
     3481 gttcaacgac cccgcagctc agagcagctt cttgcgctgg acgaagtcgg cgaggaacgc
     3541 gcggaaggcc tcgcccaggt cctcgcgctc ggcggcgagc tcgaccgtcg ccctgaggta
     3601 gtcgagcttc ttcccgatgt cgtagcgggc cccctcgaag acgtacgcgt agaccgcctg
     3661 ctcccgggcg aggatcgcga tcgcatcggt gagctggacc tccccgccgg cgtccggcgc
     3721 ggtctgtcgc agggcgtcga agatctccgg cgtgaagatg tagcggccga tcacggcgag
     3781 gttcgacggc gcctgctccg gcgatggttt ctcgacgatg tcgaggaccc gcaccaggtc
     3841 gtcttccacc gtctccggct tcacggcccc gtacaggctg atgtcctccc tggggacctc
     3901 ctggacggcg atcacgctcc gcccgtagcg ctcgtacacc tcggccatcc gcgcgagcag
     3961 ggggtcggcg gccgcgatca cgtcgtcgcc gagcaggacg gcgaagggct cccccgccgc
     4021 gtggggttcg gcgaccgcca ccgccgcacc gaggccgagc gggtcacgct ggcggatgta
     4081 gtggacggtg gccatctcgg tgatctcccg cacctgcttg aggagatcgt gcttgcccgc
     4141 gcgctcgagg tggttctcga gctcccccga gcggtcgaag tgatcctcga gcgtccgctt
     4201 gccgcggccg gtgatgatga ggagatcacg cagccccgac cggacggcct cctcgacgac
     4261 gtactggatc gcgggcttgt cgacgagggg cagcatctcc ttcggctggg ccttcgtcgc
     4321 cgggaggaac ctcgtgccga ggcccgccgc cgggatcacc gccttgttga gcttcgccat
     4381 cgcccctccg cgcgagggtc gagtgtagcc cgacccgtga cgggggtcag tcggcgactt
     4441 cgaggcggtc ccggccgctc tccttcgccc ggtacatcgc gatgtccgcg acccggatca
     4501 gctcttcggc caccgtcccg tcctcggggt agcaggcgac accgatgctg accgtgaggc
     4561 tgaagccacc cgcggcctct tcggcgaacg gcgactggcg gacgacggtg aggatcttct
     4621 ccgccacgac cttcgcgccc tcccgcggtg tctccggcag cacgacgatg aactcctcgc
     4681 cgccgtagcg cgcgagcgag tcgatctggc tgcggatcgt gctcatgatc cggcgcgtca
     4741 cctcgatcag cacctcgtcg ccgacgtggt gcccgtgcag gtcgttcacg agcttgaagt
     4801 ggtcgatgtc gatcatcagg acggaaacgg gacgtccgaa gcgctgggcc cgctcgagct
     4861 ccttcccgag cgtgagctgg aggtaccggc ggttccacac cccggtcagc ccgtcggtga
     4921 tcgagaggcg ctgcgcctcc tggtgcagga gcacgttctc gatcgcgatc gacgcctggc
     4981 ccgcgaacga gccgagcgtc tcgaggtcgt ccggctcgaa cggctgtgga gtggtccggc
     5041 cgtagagcgc gagcacgccc atgttccgct ccccgcgcgt cagggggacc gcgaccgcgg
     5101 tcgatacgac cggctccgcc gggggaaccg cctgggcgga cgtcgtgccc gacgggatca
     5161 gcacgggcat cccaccggcg gcggttcccg cgatgccctc cccgatccgc agcgagatcc
     5221 ccttcgtcgc ctcgtagccg tgaccgacct ccagctggag gcgccggccc gaaggcgagc
     5281 ggaggaacac cgcccccgac cgagcgccga gggtgaccgc cgcggtgtcg aggatcaccc
     5341 cgagcatccc ctcgaggtcg tgggtcgcgc ggagcgtcgc cccgaggcgg tcgaggccgc
     5401 ggcgcagctc gtcccgggac ttctcgagct ccccgacgta gcgccgcatg ttcgcggtca
     5461 tcgcgttgaa cgcctccgcg agccgtgcga cgtccccctg gccctcggcg gggacgaacg
     5521 tgtcgaagtc gccgcccgca acggccatgg cgccctccgc gagacgctgg agcggacgag
     5581 agaggagctt cgcgagcgcg tagccgagga gcatcgcgag cgcgaggctg atagcgatga
     5641 tgacccacat cagccctcgc atgccgcccg cacccgcctc gcgcgtcacg acggcgacgg
     5701 cggacggctg cccctcgatc ggtgtcacga gcgcacggcg gccgtcggga agcgcgatgg
     5761 gcccggagct tccagcgggg agccgggccg gcgcatcgcc gttcgaggcc accgtccgct
     5821 gacggtggac gatcacgatg tcgagcgacg cccctcgcga gagggcgcgg gcgaaccgct
     5881 cgtcggcgta gaacccaccg gtgacggtgc cgcggatgcc gtccgatacc acctcgacgc
     5941 gcgcccgcag catgggatac gcctcctcct cacggagcag gtcgcggatg cgcggcggcg
     6001 gcacctcggc tgcgtacgcc gcccgaccga cggcgtcagc gaggatcgtc tcgtcggcgg
     6061 aggtcaccac gacgaagtcg aggccggcct cgtcgcgtgc ccgctggacc tgtgcccgaa
     6121 gccccggttc gccgagcgca ccagcgaggg ccgaggcgat atgccgcacc tcacgcgagg
     6181 ctgcttccga gcgctcctgc cagagcgcag ccgcggcgcg agctccgatc tgcagtcgtt
     6241 cgtccacccg gcggtcgacc tcgcggttga cgagtacctg gaggacgacc gtggccgcga
     6301 ccagcgggac gacgacgatc atcgtgaaga acagggtgag ccgagctcgg agggtcactc
     6361 gatcaccttt ccccggccca ggcgcaggcc agcacgatcc acgatggcac cacccacagg
     6421 tatcgtcagt tcgggcggaa cccttgaatc gggacggagg ggatggatcg cgcggagcgg
     6481 aaggcggagc tgcgggcgac gatgcgcgca cggcgcgacg aggtcccggg ggaagagcgt
     6541 ctccggcggg cggaccggat cgcggggcgc atcaccgcgc tcggcgcctt ccgtgcggcg
     6601 cggacggtcc tgctcttcta ctcgttcggg tccgaggcct cgacgctgcc gatcgcgcgc
     6661 cgcgtccacg aagaggggaa gcggctcctg ctcccgttcc tgcgcgacgg cacgatggag
     6721 gccgctgagg tttcccccgg cgaagcgctc acggcctcga gatacggtcc gaaggagccc
     6781 tcgaagcgga tcgccgttga tccgcgagag gtcgacctcg tgatcacgcc gggcctcgcg
     6841 ttcgaccgcg acggcagacg actggggtac ggcgggggcc actacgaccg ctaccttacc
     6901 cgcctgcgcg acgacaccct ccgcgtgggg ctcgccttcg ccttccaggt ggtcgacgag
     6961 gtcccgtccg aggaccacga cgagcgggtg cacgtcgtcg tcaccgagga tgacgcgatc
     7021 gaagcgtcta tcatctgagc gccatgccga cctacgagta cgtctgcctc gaacgcgagc
     7081 accgcttcga ggtgttccag cggatcggag acgaacccgt cgcgacgtgc ccggagtgcg
     7141 gcagcgcggt gcggaaggtc ttccaccccg tcggcgtcgt cctgaagggg tccgggttct
     7201 acaagaccga ttcccgcggt cagaagcccg cggggaaacc tccggagggg tcctcggaga
     7261 agaagaagga gccggcctcg gagaagaaga aggcctccgg ctcctcgtcc gagggcggcg
     7321 gcacgaaggg cgagaagaag gagagcgcgt gagcgaggcg acgatcggag tgttcggcgg
     7381 gtcggggttc tactcgttcc tcgagggggt gcgggagcac gacgtggaca cgccgtacgg
     7441 caagccctcg gcgcctccgg ccgtgggtga ggtcggcgga cgccgggtgg cgttcatccc
     7501 ccggcacggc gcgcgccacc agtacccacc gcacgaggtc ccctaccgcg cgaacctgtg
     7561 ggcgatgaag gacctcggcg tggagcggat cgtcggcccc tgcgcggcgg ggtcgctcca
     7621 gcctcgcgtg aagccgggcg acttcgtgat ctgcgaccaa ctcgtcgacc ggacctccgg
     7681 ccgacgcgac accttctacg acgggccggt cacgacccac atcggcttcg ccgacccgta
     7741 ctgcccgacg atgcgcgaga tcgcgatcaa gcagggacgg gagcagggcc tcgacctgca
     7801 cgagcgcgga acggtcgtcg tcgtccaggg ccctcgcttc tcgacgcgag cggagtcgaa
     7861 gtggttcgcg agcgccggct gggaggtcat caacatgacc cagtaccccg aggcggcgct
     7921 ggcgcgggag ctcgagctct gcttcctcaa cgtctcgctg atcaccgact acgacgtcgg
     7981 ggtcgagggc gaggacatgg agcccgtgag ccacgaggcc gtcgtccggg tcttcaacga
     8041 gaacaacgcg aagctccggg acctgctgtt ccgcgtcatc ccggccctgc ccgaggagcg
     8101 cgactgcccc tgcgccacgg cgctcgacgg cgcgaggttc gaggtccact agcgttcctg
     8161 tcggtggggc gtgggatgct ccgccggtcc gctcctcccc cggaaaggag caaccgatgc
     8221 gccgcctgcg cccgcgcgcg tcccgcctgt tgttcgtctc gtcgctcttg ctcgggctcg
     8281 ccgtcacgac gaccctgaaa gggcacctcg cgcgcctgga ggcgcgcgcg gcgcgtgggg
     8341 gaccgggggt tcccgccgtc gtcgcggtgt ccgacctgcc gcgcggcacg atcctcggcc
     8401 ccgggtcgat ccgggtcgcc gaggtgccgg ccgtcgctcg cccgccgggg gcgctgtcgc
     8461 gcctggagga cgcgaccggc aagacgctcg ccgcagacgt cgcggcaggg gagatcctga
     8521 cgggcctccg cctgcgacgg ggcgggccga tcgccgcctc cctcccgccc gggctccgag
     8581 ccctcgcgat cccggtgccg gtcccgccgg ggtcggttgt cccgggggac cgcgtcgacg
     8641 tgctcgccac ctcgatggaa ggagccccgt tctccgaaac ggtggctcgc gaggtcgagg
     8701 tcctccgcgt cgaagcgagc gacgtggacg tctccgcgcc ggcgaccatc ttcgtcctcg
     8761 ccgcaccgga gacggcggag cgactggcgg cggcaaccgc gttctctcag ctctccatcg
     8821 cggtggtctc cagcgcctcc ccatagactg ccgcgcatgg tgcgcgcggt cgtcctcggc
     8881 gccgtccagg ggctgacgga gttcatcccg atctcctcat ccggccatct gacgatcgtc
     8941 ccgttcctgc tggggtggga gcatccgggc ctgccgttcg acgtcgcgat ccacctcggc
     9001 accgcgctcg cggtgatcct ctacttccat cgggagctcg tcgcgctcgt cgccggcacg
     9061 gggaagaccc tccttcggcg cggcgacgcg aacgaccgga accacgcgcg gatcgcgctc
     9121 ctgctcttcg tcgcgtcgat cccggccgga ctggcaggca tgctgttcga ggggttcttc
     9181 gaagagcggt tcggcgaggc ccggaccgca gggctgctcc tgttcgtcac cgcgggcctc
     9241 atcttcggcg gcgaagccgt ctaccggcgg cgcgacccgg gcgtgaaccg cgacctcagc
     9301 cgagtcgggt ggcgcgacgc gatgctcgtc ggcctcctcc aggcctgcgc gatcttcccc
     9361 ggcatctcac ggtcgggagc gacgatcgcg gcgggcctcg tcaccggcct ggcgcgcgac
     9421 gcggcggcgc gcttctcgtt cctgctcggg cttccggcga tcctcggtgc gggcttgctc
     9481 caggtgccgc agctcccgcc cgggatggac acgggcaccg tcgtggccgc cacggccgtc
     9541 tcggcggcga ccgggttcgc cgcgatcgcc ttcctcgtgc ggtacctccg ggcgcgcgac
     9601 atgcggccct tcgcctggta ctgcgtgctc ttcggcgccg tcgccctcgg attctggttc
     9661 caggtcagct gaggggctac gtgcccgcga ccgtcatctc gccgagcagg agggtcggtg
     9721 acccgacgga cccgccgaag aagcggaggt cgttccccgc ggcccggacg gcggtgagca
     9781 tctcgacgag cgtgctcgcg atggtcatct cgcgcagcgg ctcgccgagg atgccgttct
     9841 cgatccgcag gcccgtcgcg ccgacgctga agtccccgct caccgggttc gcccccgaat
     9901 gcacccccgt gacgtcctgc acgtagaccc cgcccccggc gcgctgcagc agggcctccg
     9961 gatcttccgc ccccgcctcg aggaagaggt tcgtcggtgc gacgcccggc cccccgcggt
    10021 aggagccgcg gcgggcgttg ccggtggagc gcgcatcggc tcgccgcgcg gtctccgagt
    10081 tgtggaggaa agcgcggagc acgccccgct cgatcagcgg ggtccgcgag gtcggaacgc
    10141 cttcgtcgtc gaacggcgcg gagcccgggc cctcgggcag gcgtccgtcg tcgatcagcg
    10201 tcacgacctc ggagcccacc gtctcaccca tccgctcggc catcatcgag cgccccttct
    10261 gcacctcttc ggcggagagc gtcctcgata ggacgccgag gaacgacgag gccgcccacg
    10321 ggtcgagcac gaccgggatc cgctccgtcc gcggcttgcg cccgcccagc agtcgcgcgc
    10381 cgcggatcgc cccctccgcc gcggcctcct gccactcgag gtcgtcgacc tcccgggcca
    10441 cccggaacga gtaaccggac tgcgtctcgc cgtcgcgctc ggccagcgcc gcgacgaggc
    10501 accagcagtc ggtgcgcgcg tattcggcag ccacacctcg cgtcgaggcg agggcgacgc
    10561 gggagatcgc gtcgccgtag cggacctcct cgaccttcgt gacggatgga tcggtcgaga
    10621 cggcgacgcg ctcgaggtcg agcgcgagcc tcaccttccg ctcgggatcg agcgacgcca
    10681 tcgatccgcg gaacaggccc gggagctcgg tgggatcggc cggggagggg agcccgttcg
    10741 cctcatcggg cgttgcgtgg cgagcgttct cgcgcgcccg ctcaagcagg ctcgccgcct
    10801 cggcgggatc ggggtcggcc gcccaggcgt agcccagccg cccctcgacg acgaggcgga
    10861 cgccgacgcc cctcgactcc gagccggcga gcttctggac gacgccgccg cgggcctcga
    10921 tcgaggtcga acggctctcc tcggcgtacg cctcgacctc ctcgcgatcg gtcgcaccgg
    10981 cgatcgccgc ccggcacagg tctaggaggt cagccaccgg ttcccccgac cgtcatgcgc
    11041 gcgatccgca gcgtcgggct tcccgtcgtc accggggcgg actggccgtc cttgccgcag
    11101 gtgccggggc gcgtgtcgaa gtcggagccg accgcgtcga cggcctgcat gatcgcgatc
    11161 ccgttgccga tcaggttcgc cccgcgcacc ggcgccgtga tcgtcccgtc ctcgatcagg
    11221 tacgcctccg agacgccgaa gacgaagtcc ccggtcgccg ggttcacctg cccgcccccg
    11281 agcgccttcg cgtagaggcc gcgcttcgtg tcgcggatga tctcctcggg gtcggtctct
    11341 ccgggaagga tcgaggtgtt cgtcatccga gggatcggca cgctcgcgta ggactggcgc
    11401 cggccgttcc ccgtcgaagg ggtgccgtcg cgacgggacc atgtgccgtc gtagagcgcc
    11461 cccacgagcg tcccgccctc gaagagggtc gtgcgctccg ccgggtggcc ctcgtcgtcg
    11521 aaggagaacg aaccccaggc gcctgggacc gtcgcgtcgt cgaccccgtg gacgagcccg
    11581 gaggcgaggc gctcccccat ccggccccgg tagaccgacg cctccttccc gatcgtgtcc
    11641 gcctccaggc cgtggccgca cgcttcgtgg aagagcacac ctccgccggc cgctccgacg
    11701 accacggcca tctccccggc cggcgccgga cggccgtcga gcatcgcgat cgcctggcgg
    11761 gccgccttcc gcgcgatctc cgaaggcggg tgctgctcca gcagctcgac cccggcgagt
    11821 ccggccggtc cctcgaaccc ggtctgcatc acgccgtcgc gcgcggcgac gacgttcacg
    11881 atcaggcgga cgcggttccg ctcctcctcg acccagcgcc cgtcggaggt cgcgatcagc
    11941 agccgctgca cggagtcccc gtagccggcg acgacctgcc gcacctctgg ggcgtgcgaa
    12001 cgtgcggcgt cgtcggcttc gcgcacccag tcgaccttcc gctccgtcgg cacctcgacc
    12061 gccggattcg cgaccggatg ccggacgggc ggctccatcc gccgaaggtc gatcacccgg
    12121 ccccgttcgc tgccgcggac cgcggccgcc gctgcctcgg cggctcgccg gagggcctcc
    12181 ggctcgacgc ggttcgagta cgcgtagccg gtcgtctcac cggcgatcac ccggacggca
    12241 gcgccgcggt cggtccccgt ggagagctcc tcgacccgcc ggtcgtcgag ccgcaccgag
    12301 gtggagacgc gctcttcggc gtagacctcg gcgaagctcc cgcccgagga gagggcgacg
    12361 tcgaggatct cgcggaccag ggactcgtcg agcatgaggg cgcatcctac cagcgggccc
    12421 ctgctaccgt gctcgcgtgt cgtggaggcg aggggccggg ttcgcgctcg cggcggtcgt
    12481 cgcccccgcc gccgtctacc tcgcagacgt ggcggcgctc gggcaccggc ttccggtggg
    12541 ccccgacggc ccgctgtaca cctggtggac gcgctacgcg ggcgtcgcgg gcctcggagc
    12601 gatcccgggc cggcccggcg ccgtagccct gacgctcggt ctcggcgacc tgttcggaag
    12661 cgacgcgtcg tcgacggtgg ccgccgccgg gcccgtcctc tcgatcgcgg gggggctggc
    12721 ggccgcggcg accgtgcacc tcctgctcgg gtccgatctc ttgcgctccg gcgccgccgc
    12781 gttgttcgtg tcggcgtggt ccgcccacct cgcggccagt tggatcgcga gcctcctgct
    12841 cgccgtcctc ttcctcgccg cggtcgccac cctcggagag ccgcgcggct cgtcgcggac
    12901 ggtggggctc ggggcggcgc tcctggccgc ggcgggtctc acgcaccgct cgatgttcgt
    12961 cctgatcgtc gtcgtcgtcg gggcggcggc cttcctcctg ctgcgcggcg gatcggcgcg
    13021 cgcccaggcc gggcggatgc tgatcgggct cgggctcggg ggcggcgtcg cggtcggcgg
    13081 gcatttcctc ctgcgggcgg cccccgcctt cggcgacacc tcacaggacg cgttcctgcg
    13141 gcgcaccgga ctgcggcaca tcctcgcgga ccgcttccgg gaacgcctcg cgggggacgc
    13201 ccgccgggcc gcgctgccgg cggcggccgc cggggcgctc tggcggctcg gccgggcggg
    13261 gtggtcccgg cgcggagaag ttttcgccgc ggtctgcctc agctgggcgg gcctgacggc
    13321 cgcggggatg ctcgccctgg ccttcgtccc gcgggcgcag gggaaccgcc tcgtggcgtt
    13381 cgcgttcttc gtgccgctcg tcgccgctcc ggggttcgcc tggctctggc ggcgcgggcg
    13441 gcaggtcgtc gccgtcgcgg cggcggcggt cctcgtgggc ggagccttcc tcgggtggct
    13501 ccgacaggac gtctacgtcg gcgcgcacga gctgcggctc gccgccgccg cgggcgaggt
    13561 cgtgcgcgag cttccgccgg ggacgccgat cgtcttcctc gtcgaccatc gtgacgacgc
    13621 ggcatcgttc cacgtcacgc gctactggaa cgtcatccgc tcggccatcc cgccggagcg
    13681 gatcgccgac gtcgtggtca gcgtcggaag cccgagcgac caccgcgccg gacgtccgac
    13741 cctcacgggt gacgaggagc acgacgagct ctcgcgccgc ttcctgcgac cgccgccgga
    13801 gccacccggc gtcacgatcg tcctgcgacc gttcaaccgg ccctggtacg ccgaggctct
    13861 gcgcgacggg accgaggtga gcggcggcgt gatcgtgctg ggcggccctc gcaccgacct
    13921 cggcgccgtg acggcggggc ctggggaagg cggggcgccg gcctgggtat cgcaggcgat
    13981 cctgatcggc gggttcctcg ccgggctggc cctcgtcggc ttcggttgga cgcgctgggc
    14041 cctccgtcga cgtgaccgtc cggccgcgct ctggttggcg ccctcagccg gagcggccgt
    14101 cgtcgtgctc ggcgccgtgg gcgccgacgc gtttgcggtc ggtcacctgc gccggtgggc
    14161 gatcgtcggg atcgcgatcg ccggctatct cgccgcctgg ttccttcccc gacaccacga
    14221 actcggcgag cagggggtcg gcgtcgccgg gcaggggacc gatcctcgtt cgacggcgaa
    14281 ggtcgcggac gacgaggatc aggaggacga gcgcgagcgg tgaggccacg tagtgcagcc
    14341 cgaggatcat cgcctcccac gcgagcggcg cgcgggaggg ctccgcgacc acctcggtga
    14401 cggcgagggc cgccgcgagc gccacggcgg cgttgcgcgg gaccttcggc aggatccagg
    14461 cgaccggcag caccaggtag gagtaccagg gcagcagcag cggcgccgtc atcaacccga
    14521 tcagcgacgc ccaccccacg gccgccacga tgagccgagg gtggacccgg gccgggtcgc
    14581 gggcgaggtg acgcgcgatc atcgcgagac acgcgagcag aacgaggggg aacaggatcc
    14641 ggaacccggc gccgacgagt cgcccgagct ccggctcacc cagcgcacgc ccgatgcgga
    14701 tcgcggtgac ccggagcaac cgcgtcggag ccagccaccc ctgcacgccg gcgaggtcga
    14761 gcatcccgag cgtcggtccg gcagtcagca tgaaggggcc ggcgagcacg gcgacgagcg
    14821 aggccgcggc tcccccgtgc atcccgagcg cgcggagccg ctccccccgc ggccgacgca
    14881 cgaccacggc accgaccgcg accgcgagcg ggagaagggc cgtgatcttc acgagcgccc
    14941 cgagggtcag caccaccgtc gcggccagct gccgcccctt gagtaggagc agcgcgccac
    15001 ccgccagggt cagcgcgacg aggggatcgt tgtgtccccc tccgacggtg tgcaggatga
    15061 ccaccgggtt ccacccgatc agcgtcgccg cgaacaccgc ccgctcgggg cgggcgcgcc
    15121 gcgccgccgc ggtcgtcaga cccatggtca ccaggctcgc gacggcagcg atgatcttga
    15181 agatcgggat gaaggcggcg ggcgcggcgg ccatcgagcc aaccgcggcg gcgagcagga
    15241 cgaaggccgg accgtagaac gacgacgtcg cgatccagtg gtgcgagacg taggggaaga
    15301 ccggttccat cgggaactgc gagggcgtga cggcgaacgg gttcgccccg tgggccgcca
    15361 ggatccgccc atacatcccg tagctgtaga cgtcgcgcga gaacatcaac ggcatcagga
    15421 gcgcgagcgc gtgcagggcg accgccacga tcaccaccgt gcgcatgctc aggttccccc
    15481 gccacgcctc gcgcagcgcg aagaggaacg cgaggaccac cgccgcgacc gcgaggcccc
    15541 cgacgcccgc cgccgcgtcg tggctcaggc catcgagccc cagcagcgag tggagcgcct
    15601 gcaggacgcc cgccgcttgg gcatcttcgg ggagcgtcgg cgtgaacgcg gagtcggtgc
    15661 gcccactgag cacggcgacc atcgccgtcg tgacgaccac gccggtcgtg gcaaggcgtg
    15721 cggcggaacg cgacaccgct gcggatgcta ccgccgtggt cagcggccca ggatctcctt
    15781 cttccgcagc tccatccgct ggccgagctc ctcgagctcc tccttctcga agacctggcg
    15841 cgccgcgtcg aacatctcgc cttcctcttc ctcgatgtgg tgctcgatgt tctccttcat
    15901 caccgagaac ttcgcgcccc aggtctcgtc gtcgtacggg gtctcgtcga tctcggagga
    15961 gatcaggtcg accacgtggt gctcctcgta cccctcgagc acgacctcct tggccttcgg
    16021 gtgctcttgg agcgccgggt agaagatctc ctcctcgatg gcttcgtggg cggctagctc
    16081 ctccttcacc ttcgcgaaga gctcttcgcg ggtcttcaca ccgcgctcgg tcgtggagcc
    16141 gagcttctcc agcaatccct tcatcgactc gtggtcgtcc ttcagcagct tcagcgcgtc
    16201 catgggtgcg tctccttctg gttcgggttc ggcgaggttc ttcccccttc gccgcggcgt
    16261 cacgctccgc ctcagggatc gatcacgtag ccgaacccgc ggcggttgcg gatgagcggc
    16321 cgggcccggt ccgcgtcacg gatgcgccgt cggagccggt agacgacacg acgaacgtcc
    16381 tcgggggtga cgcaggtgcc gtcgtcccaa aggaccgagg ccagctcacc ggcggtgacc
    16441 ggctccccgg gccgtgccgc gagggcggcc agcagcgcga gctcctgcgg cggaagctcg
    16501 tggcgcacac catcgacgaa ggcgatccca cgtcgcggat cgacacgaag cacgtccgat
    16561 ggcacggccg aacgacgacc gtcggctgag cccaggccga gcatcgcctc gagggtcctg
    16621 acgcgcgcgt cgagcccttc ggggggctcc gcgcggaaat ggaagtgatg gaccgacgcc
    16681 tcctcgagga gatcgcgttc cggcggagtg atccgcggcc gcatccagtc gggacccgcg
    16741 taggcgcgga ggcctgcggt gtcgcgccac gtggtgagca tcgcgaactc gcgcggctcc
    16801 ctctccgtcg ggtgtcccac gcgcgcttcc tcgacccttg gatcggcgcg gaacatcgga
    16861 gcagcctggt cgtggaggag gcgttcgaag gccccctcca tccccggtcg cgcacgcccc
    16921 cggaagaccc ggatgatcac tcgcccatcg tcccaccgaa gcgacatcgg cgcgacatcg
    16981 gggtcatggt ccgagacgca cgcgggtggc acgatggaga agatgacccg gggatcggtc
    17041 tggctggcgg ccgcgctcat cctcgccgcg tgccccgcag gcgaggaacc gtcgccgacg
    17101 ccgagcgtcg gcccgaccgc cgtgagccgg ccctatccct acacgacacc gacccctccg
    17161 gccgaaccca cggagatcga cgggtggtac gcgcgccagg tcgggaagga cgtcgccggc
    17221 ggtcccgggc cctgtcgccg gtgcccgccg taccgcctcg aggtcgggac ggcgacgctc
    17281 agcttcgacc gtggggtctt ccgcgtgacg aacgaccccg cggaggaagg tgcgctggca
    17341 tggaagagcg tcggccacta cgaggtcgag ggccacgagg tcgtcatctt caacgacccg
    17401 aactgcccga ccgcccgcgg ccggtaccgg tgggcgctcg cgggtggcga gctcaccctc
    17461 gaggtcgtcc aggatgactg cgccttcggc gggctgcgct ggcgctacct gacggcggcc
    17521 gcctggaccg ccgcctgagg agcggccgcc gtcagcggcc cgggcgcgga agcccgcgca
    17581 cgtacggagg agccgggatg gcgaggtcga gcttcgggtc cggctcgggg tcgcccgcca
    17641 ccgttcggac gggcaccacg ccggcccaga tcgggagcgc gtagtcgtcc tcttcgtcct
    17701 tcggtggacc gctccggacc ttcgcggaca cctcgtcgag gccgatcgcc atgatcgtcg
    17761 tctgccgaag ctcccgctcc gtcgggaggc gcacgtcagc cgagcggccc gggatcacgt
    17821 gatcgacgag cacgcgttga gcgcgctcct tctccgccgg gtccgtgacc tcccgcatgc
    17881 ggccgtagac gacgaccgag cggtagttca tcgagctgtt gaacccggag cgcgcgagga
    17941 cgagcccgtc gaggagggta acgacgatgc atgcctcgct gccctccttc agggcccgca
    18001 gggtccggct cgcgttcgac ccgtggatgt acagcgtgtc gccgtcccgt gcgtggatcg
    18061 tcggcaccac ccgaggcgcg tcgtcgacca ccaccgcgac gtggcagtag agcgcctcgt
    18121 cgaggatcgc ctcgatgacg tcgcgctcgt acaccccccg atcggggaga cgcctgacct
    18181 tcgtccgctc gctcggtggt ggctccatcg aacccagcgt acgacgtgcg ttctgcgagg
    18241 atgcaccccg tggacggaag tcacgaccct ggctccccgg ccgtcgatcg cggggacgca
    18301 cccaccatcc ttccccggca cacgtggggg aaggacctcc cgccggtcgg accgctcacc
    18361 gcggaacgcc ccgaagacgt gcgctttctg ctcatccacc acaccgcggg gtcgaacgac
    18421 taccgacccg agcaggtcat ctcgatcctc cgggggatct acgacgtgca cacgcgagag
    18481 aagacctggc cggacgtcgc ctacaacttc ttcgtcgatc gcttcggcgg catctgggaa
    18541 ggtcgggccg gtagccgcaa cgccgcgatc cgcggcgaca gcacgggcgg gcatgtgggg
    18601 ggcagcgtcc tctgctgttt cgtcgggcac caccaggagg agccacccgc ggagcctgcg
    18661 ctcgcagcga tgtcccggct cttggcctgg ctgtgccggc ggcaccagat cgacccatca
    18721 ccgggcgcca ccgtcacctt cgtctcccgc ggatcgaacc ggtggcctcc cggtgccagc
    18781 ctcacggtgc gcacgatcgc cggccaccgg gacgtctcgc gtacggtctg tcccggcgat
    18841 cagctctacc ggatgctcga gagggacctc cccgatcgag ttgccgccct cttgccgctt
    18901 ggctcatctg gcgggattga cccaccgagc ggtgcctgct agcgtcggcg cggtgtcgag
    18961 agacgagctg gtcgaggcgt acgtccaggg gcgggtcacc agacgggtgt tcatccgccg
    19021 cctggtcgcg ggcggtgtct cgctgagtgc agcggtcgtg tactcgcagc tcctcgggtc
    19081 gcaggcgcca gccgcccagc acgagggcgt ctacccggtc tacccgccac cggtcccgcc
    19141 caacaggaac cgcgtccgaa ggcagcagac cgacccagcg cagccggtcg acgtggaccc
    19201 tcgcctcacc ggctagcgcc cgggcgggag cgacgacgcg gcgatgggcg tcgaggtcct
    19261 ctgcctgctg ccggtcagga acggcgccct g
//
LOCUS       DATLGG010000139         9303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_4688, whole genome shotgun sequence.
ACCESSION   DATLGG010000139 DATLGG010000000
VERSION     DATLGG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4688"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VM638_04040"
     CDS             <1..397
                     /locus_tag="VM638_04040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVM11634.1"
                     /translation="LAILTWVLFIVTGVRHQGPAYFKNALFPPGVPKAMYILVTPIEL
                     VSTFLVRPLTLSVRLLANMMAGHLVLAIFFLGSAYLFAKPSTWAFAVPSFALSVALIA
                     FEILVAVLQAYIFTILTAVYVAGAMHPEH"
     gene            415..672
                     /gene="atpE"
                     /locus_tag="VM638_04045"
     CDS             415..672
                     /gene="atpE"
                     /locus_tag="VM638_04045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005392137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HVM11635.1"
                     /translation="MGELFANLGALVAQVEGDVGVIGRGLVFGLAAIGPGIGIGFLVG
                     KAVEAMARQPEMAGQVRTTMFLGIAFTEALALFGFVLSFII"
     gene            686..1285
                     /gene="atpF"
                     /locus_tag="VM638_04050"
     CDS             686..1285
                     /gene="atpF"
                     /locus_tag="VM638_04050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVM11636.1"
                     /translation="MTYVVKGLPRVYAQEETGTEQHAPAKEEEHHNPILPETDELVFG
                     SLAFLLVFLALWRFAFPAISRGLEQRSQKIQGDLEEAESARTQAQGMLTQYQARLDEA
                     RTEANRIIEEARKTAESMRRDVLAKAEEESRQIVARAQEEITAERDRAFEELRRQVGE
                     LSVELAERVVGHSLDKERHLQLVDGYIDELAGEGGRGAS"
     gene            1272..1814
                     /gene="atpH"
                     /locus_tag="VM638_04055"
     CDS             1272..1814
                     /gene="atpH"
                     /locus_tag="VM638_04055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HVM11637.1"
                     /translation="MARRDDLVRGYAEALFAIAEAEGVLDTVEDELFRFARALDQQDE
                     LRSALTDLALPAERKEAVLNEILGDAANEHTLNLLRFIVSSGRARDLSAIVDRVVELA
                     SERREREVAEVRTAVELTDRQRERLVAALERATGKRIDLRILVDPSVIGGVVARVGDQ
                     VLDGSLRRRLELAREHVIGA"
     gene            1816..3360
                     /gene="atpA"
                     /locus_tag="VM638_04060"
                     /pseudo
     CDS             1816..3360
                     /gene="atpA"
                     /locus_tag="VM638_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            3580..4470
                     /locus_tag="VM638_04065"
     CDS             3580..4470
                     /locus_tag="VM638_04065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HVM11638.1"
                     /translation="MGATLRTVRRRIRSVQSTMKITRAMELIAASRILKAQQRVLAAR
                     PYAELLTAAMEDVARQTGSLVHPLLEERPEPRRAAIFLVTSDRGLAGAYNANVLRRAE
                     GLMNGLRARGIEPVPYVAGRKGQSYFRFRSIHMARTWQGVSEVPSYAAAEEIAGALIG
                     AFSDREVDAVHAVYTDFRSAFTLRPIDKRFLPIAAEEVVARNGAPSPEYIFEPAPGLI
                     LDALLPRYVTAKIFHAMLESAASENAARRRAMKAATENAEEVIRRLTRVANQARQSEI
                     TTELMEVVGGAEALQQASNE"
     gene            4484..5920
                     /gene="atpD"
                     /locus_tag="VM638_04070"
     CDS             4484..5920
                     /gene="atpD"
                     /locus_tag="VM638_04070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HVM11639.1"
                     /translation="MANDKEQRPDGRVVQVIGPVVDVEFPPAALPEIHTALELDTTLG
                     GETTTIVCEVAQHIGESVVRAVALKPTDGVVRGSAVRNTGAPISVPVGPNVLGHVYNV
                     LGQPLDTDSVEYDERWPIHRDPPKFEDLEPKTQMFETGIKVIDLLEPYVQGGKIGMFG
                     GAGVGKTVIIQEMIYRVAEQHGGVSLFAGVGERTREGNDLFLEMTESGVINKTALVFG
                     QMDEPPGVRLRVGLAALTMAEYFRDVQRQDVLLFIDNIFRFVQAGSEVSTLLGRMPSA
                     VGYQPTLADEMGELQERITSTKGRSITSLQAIYVPADDITDPAPHTTFAHLDATTVLS
                     RQIVELGIYPAVDPLDSTSRILDPRYVGEEHYAVARRVQEILQRYKNLQDIIAILGID
                     ELSEEDKVIVARARKIQKFLSQPFFVAEQFTGIPGKYVSVADTIRGFKMIADGELDHL
                     PEQAFYMVGGIEEAIEQGEKLAKVGAEA"
     gene            5923..6198
                     /locus_tag="VM638_04075"
     CDS             5923..6198
                     /locus_tag="VM638_04075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HVM11640.1"
                     /translation="MALEVRVVSPEREVWSGEAELVVAKGVDGDVGIMQGHAPMLVSL
                     AVHPIRITRPGGEVQVVEAQGGFLHVTPGPETTRVDILAEAATLPEA"
     gene            complement(6250..7401)
                     /locus_tag="VM638_04080"
     CDS             complement(6250..7401)
                     /locus_tag="VM638_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11641.1"
                     /translation="MRRIALLGLAVATLVSGVPASAVPETSVDSPYGREDNPLGTIRW
                     LPRNANESPTATNRFRLVVVYDRSLNDAGQPVALKAKGAFGVKIFIPTVDARVMARSP
                     QIQDVRDFTGQGGIPPEVDQRAMAFAGDVRDGDCAKAGTPAFSAAYSTTRSFYHAGAT
                     PYQPISAAGTCEVVTRSEPATDSEGNVVGYETTVTNHVPGFWVDVEWPTRAIPGLGGA
                     GRWNAEVWYGAVYDSGGDPGPFPNGVDTSGSSKSLPAPGDYDQVFSFTAQPGAVGAEC
                     VNTGGNNRPMPGTFHDVKIMVPQAAKRVVFKLSNHVDWDLLVFNPNGLKRTSALAPPL
                     DETITAPGPTPNTSTSHANFPDLVPGEYTMRACNFAGEPTTIGGVIIEF"
     gene            complement(7540..8976)
                     /locus_tag="VM638_04085"
     CDS             complement(7540..8976)
                     /locus_tag="VM638_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11642.1"
                     /translation="MRTTLRVALLGILALSLAFVAMPAGADPEGDHPGSGAGDPGSHE
                     KMQHLANVPRSSDQFQSDLAFVGKYAFAGNYNGFRIIDVSAPARPRVVKDVWCPGPQN
                     DISVWGDLVILSVDSVMVNDRCGSPVSPNLHQGWEGIRIFSLSEVLSTPAQPNGFTVV
                     QPVATAYTDCGSHTHTGYPQGDTLYVYISSYPLRGGPGCGPNNPQGENPLHEKISIVE
                     VPVSNPTALEVHEVPIDIPTFNQDPMFLPPPAFNPMRGCHDIQVHVGLELAAAACSSV
                     GQVWDISDPLNPTTLDPLWEVDEPEVQFYHSALFSEDAETVIFGDEIITGANCDDGSG
                     AGQMWFYDMDGTLLSSHQTPRHQGGAYCTAHLFNNIPTNRGNVLVASWYAAGTAVIDY
                     TDPSNPREIAHFDPANTDTLRTSTWASYWYNGFVYANDGTGTRPNPSPPPATLHSSRG
                     LDIFSISDKVKAAAGAKKVQVLNPQTQY"
BASE COUNT         1437 a         3098 c         3358 g         1410 t
ORIGIN      
        1 cctcgcgatc ctgacctggg tgctgttcat cgtcaccggg gtccgccacc agggacccgc
       61 gtacttcaag aacgcgctgt tcccgccggg cgttccgaaa gcgatgtaca tcctcgtgac
      121 gccgatcgag ctcgtctcga cgttcctcgt ccggccgctc acgctgtcgg tccgactcct
      181 cgccaacatg atggcggggc acctcgtgct ggcgatcttc ttcctgggct cggcctacct
      241 gttcgcgaag ccgtccacct gggcgttcgc ggttccctcg ttcgcgctct cggtggcgct
      301 gatcgcgttc gagatcctcg tggccgtgct ccaggcctac atcttcacga tcctcaccgc
      361 cgtgtacgtc gccggggcca tgcaccccga gcactgagtc aaggagaacg gatgatggga
      421 gagctgttcg cgaacctcgg ggcgctggtg gcgcaggtcg agggagacgt gggcgtgatc
      481 ggacgcgggc tcgtcttcgg cctcgcggcg atcgggcccg gcatcggcat cgggttcctc
      541 gtcggcaagg ccgtcgaggc gatggcgcgc cagccggaga tggccgggca ggtccggacg
      601 acgatgttcc tcggcatcgc gttcaccgag gcgctcgcgc tgttcggctt cgtgctgtcg
      661 ttcatcatct agtcgggagg cggcgatgac ctacgtggtg aagggcctcc cgagggtcta
      721 cgcccaggag gagacgggca ccgagcagca cgccccggcg aaggaggagg agcaccacaa
      781 cccgatcctc cccgagaccg acgagctggt cttcgggagc ctcgcgttcc tgctcgtgtt
      841 cctcgcgctg tggcgcttcg ccttcccggc gatctcgcgg gggctcgagc agcggtcaca
      901 gaagatccag ggtgacctgg aggaagccga gtcggctcgg acgcaggccc aggggatgct
      961 cacgcagtac caggcgcggc tcgacgaggc gcgcaccgag gcgaaccgca tcatcgagga
     1021 ggcgcggaag acggccgagt cgatgcgccg cgacgtcctc gcgaaggccg aggaggagtc
     1081 gcggcagatc gtggcgcgtg cgcaggaaga gatcacggcc gagcgggacc gggcgttcga
     1141 ggagctgcgc cgccaggtgg gtgagctctc cgtggagctc gccgagcggg tcgtggggca
     1201 cagcctggac aaggagcggc acctccagct cgtcgacggt tacatcgacg agctcgccgg
     1261 agagggcggt cgtggcgcgt cgtgacgacc tcgtccgcgg ctacgccgag gcgctgttcg
     1321 cgatcgccga ggcggagggg gtcctcgaca ccgtcgagga cgagctcttc cggttcgcgc
     1381 gcgccctgga ccagcaggac gagctgcgct ccgcgctgac cgatctcgcc ctgccggccg
     1441 agcggaagga ggccgtgctc aacgagatcc tgggagacgc ggcgaacgag cacacgctga
     1501 acctgctccg tttcatcgtc tcctccggcc gcgcacgcga cctgtccgcg atcgtcgatc
     1561 gggtggtcga gctcgcctcg gagcggcgcg aacgggaggt ggcggaggtt cggaccgcgg
     1621 tcgagctgac cgaccgccag cgcgagcggc tcgtggcggc gctcgagcgg gcgacgggga
     1681 aacggataga cctgcgtatc ctggtagacc cgagcgtgat cggaggggtc gtcgcccgcg
     1741 tcggcgacca ggtgctggac gggagcctca ggcgccggct cgagctggcg cgtgagcatg
     1801 tgatcggagc gtgagatggc tgagctgacc ctcgacccac agaagatcgc agacgcgctg
     1861 cggcggcacg tcgaaggctt cgacccgcac gtcgaggccg aggaggtcgg gcgcgtccag
     1921 gacaccggcg acgggatcgc ccgcgtcacg gggctcccga acgcgatggc gaacgagctg
     1981 ctcgagttcc ccggcgggat cctcggcgtc gccttcaacc tcgaggagga ccagatcggg
     2041 tgcgtcgtcc tcggcgaggc cgagggcatc gaggaggggt cgccggtcaa gcggaccggg
     2101 cgcatcctct ccgtcggcgt cggcgacgcg gtcctcggcc gcgtcatcga ccccctcggg
     2161 cgcccgctcg acgggaaggg cccgatcgcg taccaggagc agcgtgtcct cgagctccag
     2221 gcgccgtcgg tcgtccagcg gcagccggtg accgaaccgc tccagacggg cgtcaaggcg
     2281 atcgacgcga tgacgccgat cggccgcggg cagcgggagc tcatcatcgg cgaccgccag
     2341 accgggaaga ccgcgatcgc gatcgacgcc atcatcaacc agcgggagaa ctgggcgacc
     2401 ggcgacccga agaagcaggt gaagtgcgtc tacgtggcca tcggccagaa ggcctccacg
     2461 gtggccgagg tcgtcggggc cctcgaggag aacggggcgc tggagtacac ggtcgtcgtg
     2521 agcgcaccgg cgtccgtccc ctcatccctc aagttcatcg ccccgtactc cgggtcgtcg
     2581 atcggccagc actggatgta caacggccag cacgcgctga tcgtcttcga cgacctgtcg
     2641 aagcaggccg aggcgtaccg cgagatctcg ctgctgctcc ggcggcctcc gggccgcgag
     2701 gcgtacccgg gcgacgtctt ctacctccac tcgcgcctgc tcgagcgctg cgcgaagctc
     2761 tcggacgagc tcgggggggg ctcgctcacg ggcctcccga tcgtcgagac gaagggcggc
     2821 gacgtctccg cctacatccc gacgaacgtg atctcgatca ccgacggcca gatctacctc
     2881 gagtccgacc tgttcaacgc cggcgtccgc ccggcgatca acgtcggcat ctccgtctcg
     2941 cgcgtcggtg ggaacgcgca gatcaaggcg atgaagcagg tcgccggccg cctgcgcctc
     3001 gacctcgccc agttccggga gctcgaggcg ttcgccgagc tcggctccga gctcgacaag
     3061 gcatcgcagg cccagctcga ccgcgggacc cgtgtcgtcg aggtcctgaa gcagctgcag
     3121 taccgtccgt tcccagtcga gcgccaggtc gtcagcatct gggccgtcac gggaggccac
     3181 ctcgacgaca tcccggtcgc ggacgcgaag cggttcgagg aggagttcct cgcctacgtg
     3241 gagacgcggc acggggacgt cctgcggcgg atcgcggagg aggggaagct cggggacgac
     3301 ctcgagtcca cgctccagac ggcggtctcg gagttcaaga agcagttcgt cgcctcggag
     3361 caggagaccg gggccggcgt cgtcgggggc ggcccgatcg accagctcca ggaggacgtc
     3421 ggctgggacc gggtcgggga gcaggcgccg gagggtgaag gcgcgcaggt cgccgccgac
     3481 cgcgaggagc cggggcgtca gctgaccgag ccgccgacgc cgaccgagcg tgaggccggc
     3541 ccggtcggcg agacgaacgt cgagcctccg gggtagcgga tgggagccac cctccgcacc
     3601 gtccggcggc ggatccgctc cgtccagtcg acgatgaaga tcacgcgcgc gatggagctg
     3661 atcgccgcgt cgcggatcct caaggcgcag cagcgggtcc tcgcggccag gccctacgcc
     3721 gagctgctga ccgcggcgat ggaggatgtg gcgcgccaga ccgggtcgct cgtgcacccg
     3781 ctcttggagg agcggcccga gccgcggcgc gcggcgatct tcctcgtcac gtccgaccgc
     3841 ggcctcgcgg gcgcgtacaa cgcgaacgtc ctccggcggg cggagggtct gatgaacgga
     3901 ctgcgggcgc ggggcatcga gccggtgccg tacgtcgccg gacgcaaggg ccagtcgtac
     3961 ttccggttcc gctcgatcca catggcgcgg acctggcagg gcgtgagcga ggtgccgagc
     4021 tacgcggccg ccgaagagat cgccggcgcg ctgatcgggg cgttctccga tcgggaggtc
     4081 gacgccgtcc acgcggtcta cacggacttc cgctcggcgt tcacgctccg cccgatcgac
     4141 aagcggttcc tgccgatcgc ggccgaggag gtcgtcgctc gcaacggcgc gccctcgccg
     4201 gagtacatct tcgagcccgc gccgggactg atcctcgacg cgctccttcc gcggtacgtg
     4261 accgcgaaga tcttccacgc gatgctcgaa tcggccgcgt cggagaacgc cgcgcggcgg
     4321 cgggcgatga aggcggcgac cgagaacgcg gaggaggtca tccgccgcct gacgcgggtg
     4381 gcgaaccagg cgcggcagtc ggagatcacg acggagctga tggaagtcgt cggcggagcc
     4441 gaggcgcttc agcaggcgtc caacgagtag acgggagaga gcagtggcga acgacaagga
     4501 gcagcgtccc gacggacgcg tcgtacaggt gatcggcccg gtggtagacg tcgagttccc
     4561 gcccgccgcc ctgccggaga tccacaccgc cctcgagctc gacacgacgc tcggcggaga
     4621 gacgacgacg atcgtctgcg aggtcgcaca gcacatcggc gagtcggtcg tgcgggcggt
     4681 cgcactgaag ccgaccgacg gcgtggtccg ggggagcgcg gtgcgcaaca ccggggcgcc
     4741 gatctccgtt ccggtggggc cgaacgtcct cggccacgtc tacaacgtgc tcgggcagcc
     4801 cctcgacacc gattcggtgg aatacgacga gcggtggccg atccaccgcg acccaccgaa
     4861 gttcgaggac ctcgagccga agacccagat gttcgagacc gggatcaagg tcatcgacct
     4921 cctcgagccc tacgtgcagg gcggcaagat cgggatgttc ggcggcgccg gcgtcggcaa
     4981 gaccgtcatc atccaggaga tgatctaccg cgtcgccgag cagcacggcg gcgtgtcgct
     5041 gttcgccgga gtgggcgagc ggacccgcga agggaacgac ctgttcctcg agatgaccga
     5101 gtcgggcgtc atcaacaaga cggcgctcgt cttcgggcag atggacgagc cgccgggcgt
     5161 gcggctccgc gtcgggctcg cggcgttgac gatggcggag tacttccgcg acgtccagcg
     5221 ccaggacgtg ctcctgttca tcgacaacat cttccgattc gttcaggccg gatcggaggt
     5281 ctcgaccctc ctcggtcgga tgccgtcggc ggtcggttac cagccgacgc tcgccgacga
     5341 gatgggggag ctccaggagc ggatcacctc gacgaagggg cgctcgatca cctccctcca
     5401 ggcgatctac gtcccggccg acgacatcac ggacccggcg ccgcacacga cgttcgcgca
     5461 cctcgacgcg acgacggtgc tgtcacggca gatcgtcgag ctcggcatct acccggccgt
     5521 ggacccgctc gactccacga gccggatcct cgacccgcgc tacgtgggtg aggagcacta
     5581 cgcggtcgcg cggcgcgtcc aggagatcct ccagcgctac aagaaccttc aggacatcat
     5641 cgccatcctc gggatcgacg agctctccga ggaggacaag gtgatcgtcg cgcgggcgcg
     5701 gaagatccag aagttcctga gccagccctt cttcgtggcc gagcagttca cggggatccc
     5761 cgggaagtac gtcagcgtgg ccgatacgat ccgcggcttc aagatgatcg ccgacggcga
     5821 gctcgaccac cttcctgagc aggccttcta catggtcggc gggatcgagg aggccatcga
     5881 gcagggcgag aagctggcga aggtcggcgc ggaggcctga gcgtggcgct cgaggtccgc
     5941 gtcgtctcgc cggagcgcga ggtgtggtcc ggcgaggccg agctcgtcgt ggcgaagggg
     6001 gtcgacggcg acgtcgggat catgcagggt cacgccccga tgctcgtctc tctggcggtc
     6061 catccgatcc gcatcacgcg gcccgggggt gaggtccagg tggtcgaggc ccagggcgga
     6121 ttccttcacg tgacgccggg gcccgagacc acgcgggtcg acatcctggc cgaggcggcc
     6181 acgcttcccg aggcgtagac atccgagagc ccggcgcgaa ggccgggctc tcgaaccgcg
     6241 ggcgcggggt cagaactcga tgatcacccc gccgatcgtc gtcggctccc cggcgaagtt
     6301 gcaggcccgc atcgtgtact cgcccggaac gaggtcgggg aagttcgcgt ggctcgtgga
     6361 ggtgttcggc gtcggacccg gcgccgtgat cgtctcgtcg agcggtggcg cgagcgccga
     6421 cgtccgcttg agcccgttcg ggttgaagac cagcaggtcc cagtccacgt ggttcgacag
     6481 cttgaagacg acccgcttcg ccgcctgagg gaccatgatc ttcacgtcgt ggaacgttcc
     6541 cggcatcggg cggttgttcc cgccggtgtt gacgcactcc gcgccgacgg cacccggctg
     6601 ggctgtgaac gagaagacct ggtcgtagtc cccgggcgcc gggaggctct tcgagctgcc
     6661 ggaggtgtcc actccgttcg ggaacggccc tgggtccccg ccgctgtcgt agaccgcgcc
     6721 gtaccagacc tcggcgttcc agcgtccggc gccgcccaag ccggggatgg cgcgggtggg
     6781 ccactcgacg tccacccaga accccgggac gtggttcgtc acggtggtct cgtagccgac
     6841 gacgttcccc tccgagtccg tggcaggctc cgaccgggtg accacctcgc aggtaccggc
     6901 cgcgctgatc ggctgataag gggtcgcgcc ggcgtggtag aagctgcggg tcgtggagta
     6961 ggcggccgag aacgcgggtg tcccggcctt cgcgcagtcc ccgtcgcgga cgtccccggc
     7021 gaaggccatc gcacgctgat cgacctcggg cgggatgccg ccctgcccgg tgaagtcccg
     7081 gacgtcctgg atctgagggg agcgcgccat cacgcgcgcg tcgaccgtcg ggatgaagat
     7141 cttcacgccg aaggcgccct tcgctttcag ggcgaccggc tgcccggcat cgttcagcga
     7201 ccggtcatag acgacgacga gccggaaccg gttcgtagcc gtcgggctct cgttcgcgtt
     7261 cctcgggagc cagcggatcg tcccgagggg attgtcctcc cgtccgtacg gactgtcgac
     7321 cgatgtctct ggaacggcgg acgcgggcac gcctgagacg agtgtcgcga cggcgagtcc
     7381 gagcagcgcg atgcggcgca atgggtgcct ccttgtcctc ggtgggctcc ggaagattcg
     7441 aggacggcgc tcggtttcct ttgtgcatct gcacagactt ctggacgcgg aagagccccg
     7501 ggctcggtgc ccggggctct tccggtcgat ccggtcggat cagtactgcg tctgcgggtt
     7561 gagcacctga accttcttcg cgccggcagc ggccttcacc ttgtcgctga tggagaagat
     7621 gtccaggccg cggctggagt gcagcgtcgc cggcgggggc gaggggttcg gccgcgtccc
     7681 cgtcccgtcg ttcgcgtaca cgaacccgtt gtaccagtac gacgcccacg tcgaggtgcg
     7741 gagggtgtcc gtgttcgcgg ggtcgaagtg cgcgatctcc cgcgggttcg acgggtcggt
     7801 gtagtcgatg acggccgtcc cggcggcgta ccacgacgcc acgaggacgt tccccctgtt
     7861 cgtcgggatg ttgttgaaca ggtgcgcggt gcagtaggcg ccgccctggt gccgcggcgt
     7921 ctggtgcgag ctcagcaggg tcccgtccat gtcgtagaac cacatctggc ccgccccgct
     7981 gccgtcgtcg cagttcgccc ccgtgatgat ctcgtcaccg aagatcacgg tctccgcgtc
     8041 ttcgctgaac agggccgagt ggtagaactg cacctccggc tcgtcgacct cccagagcgg
     8101 gtcgagcgtc gtcgggttga gggggtccga gatgtcccag acctgaccga cgctcgagca
     8161 cgccgcagcg gcgagctcga gaccgacgtg gacctggatg tcgtggcacc cgcgcatcgg
     8221 gttgaacgcc ggcgggggca ggaacatcgg gtcctggttg aaggtcggga tgtcgatcgg
     8281 aacctcgtgg acctcgaggg ccgtcgggtt gctcaccgga acctcgacga tcgagatctt
     8341 ctcgtgcagc gggttctcgc cctgcgggtt gttcggaccg cacccggggc caccgcgcag
     8401 cgggtacgag gagatgtaga cgtacagcgt gtcgccctgc gggtatcccg tgtgcgtgtg
     8461 cgatccacag tccgtgtacg ccgtggcgac cggctggacc acggtgaagc cgttcggctg
     8521 cgccggggtc gagaggacct cgctcaggct gaagatccgg atgccctccc acccctggtg
     8581 gaggttcggc gagaccgggc tgccgcagcg gtcgttcacc atcacgctgt cgacggacag
     8641 gatgaccagg tcaccccaga ccgagatgtc gttctgcggc cccgggcacc agacgtcctt
     8701 gaccacgcgc ggccgagcgg gcgcggagac gtcgatgatg cggaagccgt tgtagttgcc
     8761 ggcgaaggcg tacttcccca cgaacgcgag gtccgactgg aactgatcgg aggaccttgg
     8821 aacgttcgcg aggtgctgca tcttctcgtg cgatccgggg tcgccggccc cggaaccggg
     8881 atgatccccc tcggggtcgg cgccggccgg catcgcgacg aacgcgaggc tgagcgccaa
     8941 gatcccgagg agggcgacgc gaagcgtcgt gcgcatactc cccacctccc tgtgcggaca
     9001 cacaccgacc ccctgcgggc cggtgcgggc aagtctaggc aaggcaggca ggttaggtca
     9061 aacgtcgtac gcggggacag gcgaggaaaa agggacggcc cccgggggtc gccccggggg
     9121 ccgtcgcgct tccggcgctg ctactccttg ccggtgtagc ggaagatcca gaggccggag
     9181 tcccggtcgc tcatcagcag cagcggccgg gcgttcccct ggttcttgcc cttcgccttc
     9241 gtgttgttgc cagggagcac cgggtagacg ccccagaagt cgttgccacc ctcggcgatg
     9301 tag
//
LOCUS       DATLGG010000140         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_136074, whole genome shotgun sequence.
ACCESSION   DATLGG010000140 DATLGG010000000
VERSION     DATLGG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136074"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..895
                     /locus_tag="VM638_04090"
     CDS             209..895
                     /locus_tag="VM638_04090"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HVM11643.1"
                     /translation="MLFLLGFLGAAALLQERLLERELPGRREALEALVARRAAAVRDL
                     GRATRALEDELARVRNEAAADSGELRSLIAEVDRLSAVGGLEALRGPGLVLTVSDSRH
                     APVTREEARDFRIQDVDLQALLNAVWRAGAEAVAVNGRRVVATTAVRQAGDRILVNYA
                     PVASPYRLVAIGDAPSLARRVARSEAARRFQVWGEVYGLGFDLRTGTNLHVPALGPPP
                     NLDYASPERR"
     gene            892..1221
                     /locus_tag="VM638_04095"
     CDS             892..1221
                     /locus_tag="VM638_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HVM11644.1"
                     /translation="MTALIALVLGVVAGLVLQPTLPPGLVPYLPVVVVAALDTVLEGA
                     RARLEGGYHEGEFIVAFLANTLLAVFVVWVGDRLGAPDLRLGVIVVFGVRMFQSLAAI
                     RRHLFRE"
     gene            1218..1964
                     /locus_tag="VM638_04100"
     CDS             1218..1964
                     /locus_tag="VM638_04100"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HVM11645.1"
                     /translation="MTLDWEPVAPAWREARGPRMRPPSRLRTRLVAAALAVVIGLLVG
                     VQAGERGGAAERLRGESVEDLTLILNDLNAEADRLARQISELRVREARLRSSAGNEEV
                     LLEEARSRLEVLEVLSGQVPVRGPGVVVRIEDPAGAVSWDVILDSVQELRDAGAEAIS
                     VGDVRVVASTWFGPSEGGITVGGTPVRPPYELRAIGAPDAIREALQIAGGPLTLLQAQ
                     TGVRVSILDEQELRLPALHRPIRFRYARAA"
     gene            1980..2375
                     /gene="gcvH"
                     /locus_tag="VM638_04105"
     CDS             1980..2375
                     /gene="gcvH"
                     /locus_tag="VM638_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVM11646.1"
                     /translation="MEFPEDLKYTREHEWAALEDGGRLRVGITDFAQDALGDVVYVDL
                     PEPGTEVRGGEAFGEVESTKSVSDIFSPVSGTIVERNTALEESPELVNQDPYGEGWMV
                     LIDPADPTDADELLDAAAYQAFIREDADA"
BASE COUNT          316 a          911 c          979 g          362 t
ORIGIN      
        1 gaccgagacg atcgacggcc tcaaggccta ccggggacgc gattgggccc tcgtcgtgcc
       61 gcacccgcag gagccgctcg tccgggtctg ggccgaggcg gacagcgcgc ggcgggccga
      121 ggccctcgca tcggagttcg cggagctcgt cgaagagctc cgcacctaga ccgtggcctc
      181 ccggacctgc cagttcggtc tcggcggggt gctgttcctc ctcgggttcc tcggcgcggc
      241 ggccctgctc caggagcgcc tcctcgagcg cgagctgccg ggacggcggg aggcgctcga
      301 ggcgctcgtg gcgcgccgcg ccgctgcggt gcgggacctc gggcgcgcca cgcgcgcgct
      361 ggaggacgag ctcgctcgcg tgcggaacga ggcggcggcc gactcgggtg agctgcgctc
      421 gctcatcgcc gaggtcgatc ggctgagcgc cgtgggcggg ctcgaggcgc tccgagggcc
      481 gggcctcgtc ctcacggtct ccgacagccg gcacgccccg gtgacgcgcg aggaggcgcg
      541 tgacttccgg atccaggacg tcgacctgca ggccctcctg aacgcggtgt ggcgcgccgg
      601 cgccgaggcc gtggcggtca acggccggcg ggtcgtggcg acgaccgccg tgcggcaggc
      661 gggggatcgg atcctcgtga actacgcgcc cgtggcgtcc ccgtaccgcc tggtcgcgat
      721 cggggatgcg ccgtcgctcg cgcgccgggt cgcccggtcg gaggccgccc gccggttcca
      781 ggtctggggg gaggtctacg gtctgggctt cgacctccgc acggggacga acctgcacgt
      841 ccccgcgctg ggcccgccgc cgaacctgga ctacgcttcg ccggaacgac gatgaccgcg
      901 ctgatcgcgc tcgtgctggg ggtggtggcg ggactcgtcc tgcagccgac gctcccaccg
      961 gggctcgtcc cgtacctgcc ggtggtggtc gtcgcggcgc tcgacaccgt cctcgagggg
     1021 gcgcgtgccc ggctcgaggg cggctaccac gagggcgagt tcatcgtcgc attcctcgcg
     1081 aacaccctgc tcgcggtctt cgtcgtgtgg gtgggggacc gcctgggcgc cccggacctt
     1141 cggctcgggg tgatcgtcgt cttcggcgtc cggatgttcc agagcctggc ggcgatccgc
     1201 cgccacctgt tccgggaatg acgctcgact gggaacccgt cgcccccgcc tggcgggagg
     1261 cccgcgggcc gcggatgcgc ccgccgagcc gcctgcggac ccggctcgtg gcggcggcgc
     1321 tcgcggtggt gatcggcctc ctcgtcggtg tccaggccgg ggagcggggc ggcgccgcgg
     1381 agcgcctgcg gggcgagtcc gtcgaggacc tcaccctgat cctgaacgac ctcaacgccg
     1441 aagccgaccg gctggcgcgg cagatctccg agctgcgggt ccgcgaggcg cgcctccgct
     1501 cctccgccgg gaacgaggag gtgctgctcg aggaggcgcg gagccggctc gaggtgctcg
     1561 aggtcctctc gggccaggtc cccgtccggg gccccggggt cgtggtgcgg atcgaggacc
     1621 cggcaggcgc ggtgagctgg gacgtcatcc tggacagcgt ccaggagctc cgcgacgcgg
     1681 gggctgaggc gatctcggtg ggggacgtgc gcgtcgtcgc ctcgacgtgg ttcggcccgt
     1741 ccgagggcgg gatcaccgtg ggggggacgc ccgtccggcc gccgtacgag ctccgggcga
     1801 tcggggcacc ggatgcgatc cgcgaggcgc tgcagatcgc gggtggcccg ctcacgctgc
     1861 tccaggcgca gacgggtgtc cgggtgtcga tcctcgacga gcaagaactt cgcctccccg
     1921 ccttgcaccg gccgatccgc tttcgctacg ctcgcgcagc ctgatcgcgg ggagacgacg
     1981 tggagtttcc ggaagatctg aagtacacgc gggagcacga gtgggccgcg ctggaagacg
     2041 gcggccggct gcgcgtcggc atcacggact tcgcgcagga cgccctcggc gacgtcgtct
     2101 acgtggacct cccggagccg ggcaccgagg tccgcggggg agaggccttc ggtgaggtgg
     2161 agtcgacgaa atcggtctcc gacatcttca gcccggtctc gggcacgatc gtggagcgga
     2221 acaccgcgct cgaggagtcc ccggagctcg tgaaccagga cccctacggc gagggctgga
     2281 tggtgctgat cgacccggcc gaccccaccg acgccgacga gctgctcgac gccgccgcct
     2341 accaggcgtt catccgggag gacgcggacg cctgagcgtt gaccgctcag gctctctcgg
     2401 ctagatttgt cactccagtc tcaacctaga ccggagcgtg aggtcgagag cccatgccca
     2461 gatgccgcac ctgcggccgt gacaaccccg ccgacgcacg cttctgcgcg cactgcggaa
     2521 gcgccctgca cgatgacacc accggcagcc tcgcccccac ggaggtgc
//
LOCUS       DATLGG010000141         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_179831, whole genome shotgun sequence.
ACCESSION   DATLGG010000141 DATLGG010000000
VERSION     DATLGG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179831"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VM638_04110"
     CDS             <1..279
                     /locus_tag="VM638_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVM11647.1"
                     /translation="VEGRDIGTVVFPDADLKVFLAADPAERAARRQQERETQDPGLAD
                     ALTRRDALDARTNPLEPAQDARVVDTTGRDPAEVLDEVLALVREVVGA"
     gene            276..1619
                     /gene="der"
                     /locus_tag="VM638_04115"
     CDS             276..1619
                     /gene="der"
                     /locus_tag="VM638_04115"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVM11648.1"
                     /translation="MSTASERLPRVAIIGRQNVGKSTLLNRFLGAREAIAHEQPGVTR
                     DRLEAPVEWRGRRFLAVDTGGYVERARGIEREVARQADRAAAEADLVLLVVDAVTGVQ
                     EEDAVLARRLLRADAPVLVVANKVDNEALEHQATAFLRLGLGEPLPVSAMHGRGAGDL
                     LDRVVDLLPEEGEVEGPQEDASFALVGRPNVGKSSMFNRLVGEERAVVYEEAGTTRDA
                     IDSVVSWEGRPLRFVDTAGFRRPSRTQGVEYYGYVRAVQAIDRSHVVALVIDASEGVT
                     GEDKRIAARVAEAGRGLVAVANKWDLVAQGERAERFAMIREQIEVFPRVPLLRTSAKT
                     GAGVGKVVPALLQVHGEWTRRVPTAEVNRVLHRAQAEHPPPRGGGRIRYGTQVASGPP
                     RFVVFATGSRQQVSYRRYLENRFREAFGFDGVPIRISFRGRDRSRSGGARRAAGE"
     gene            1652..2257
                     /locus_tag="VM638_04120"
     CDS             1652..2257
                     /locus_tag="VM638_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVM11649.1"
                     /translation="MTGHRVPSGREHLAALARPLAALEGDVARIEGWGADLAEILLAG
                     GRLLAAGNGGSAAQAQHLTSELVGRYGVDRPALSAIALHAETSSLTAIANDFGLEEAF
                     ARQVRAHGRPGDVLVALSTSGRSGNVVAAVDEARRGRMRIWALTGPVPNPVADGADDA
                     VVVDAPATATIQEVHQVAIHILCAAVDATLVERGLTPRVEA"
     gene            2395..2811
                     /locus_tag="VM638_04125"
     CDS             2395..2811
                     /locus_tag="VM638_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HVM11650.1"
                     /translation="MDRTAPRRGYARTFGWIWGIAYLAVALLELLTMNNGLQVGGTEI
                     LRFGALHNGIHWATGIALIGSAMAGEGAARLVCRIIGVAFVAVVIWNFISPDSYARFV
                     GMGDDLPVSYILVHIITALGALFAGFAGPATDRDRT"
BASE COUNT          382 a          991 c         1155 g          409 t
ORIGIN      
        1 gtcgaggggc gcgacatcgg aacggtcgtc ttccccgatg ctgatctcaa ggtcttcctc
       61 gcggcggacc cggcggagcg ggcggcgcgg cggcagcagg agcgggagac gcaggacccg
      121 ggtctcgcgg acgccctgac ccggcgcgat gcgctcgacg cgaggacgaa tccgctcgag
      181 ccggcgcaag acgcgcgcgt cgtcgacacg accggccgcg acccggcgga ggtgctggat
      241 gaggtcctcg ccctggtccg cgaggtcgtg ggagcgtgag cacggcttcc gagcgcctgc
      301 cgcgcgtcgc gatcatcggc cgacagaacg tggggaagtc tacgctgctc aaccggttcc
      361 tcggcgcgcg ggaggcgatc gcgcacgagc agcccggggt cacgagggac cggctcgagg
      421 ctccggtcga gtggcgcggg cggcggttcc tcgccgtgga cacgggcggc tacgtcgagc
      481 gtgctcgcgg gatcgagcgg gaggtcgcgc ggcaggcgga ccgggctgcc gcggaggccg
      541 acctcgtgct gctcgtcgtg gacgcggtga cgggggtgca ggaggaggac gccgtgctcg
      601 cgcggcggtt gctgcgcgcc gacgcccccg tgctcgtcgt cgcgaacaag gtcgacaacg
      661 aggcactcga gcatcaggcg acggcgttcc tgcggctcgg tctcggggag ccgctcccgg
      721 tctccgcgat gcacggacgg ggtgcgggcg acctgctgga ccgcgtcgtg gacctcctgc
      781 cggaggaagg agaggtcgag ggcccgcagg aggacgcgtc gttcgcgctc gtgggacgcc
      841 cgaacgtggg gaagtcgtcg atgttcaacc gcctcgtggg cgaggagcga gcggtcgtct
      901 acgaggaggc ggggacgacg cgtgacgcca tcgattcggt ggtctcgtgg gagggtcgcc
      961 cgctccgctt cgtcgacacc gccggcttcc gccggccgtc ccgaacccag ggcgtggagt
     1021 actacggcta cgtccgtgcc gtccaggcga tcgaccgcag ccacgtcgtc gccctcgtga
     1081 tcgacgcctc ggagggcgtc acgggagagg acaagcggat cgcggcgcgc gtcgccgagg
     1141 ccgggcgcgg gctcgtcgcg gtggcgaaca agtgggacct cgtggcgcaa ggggagcggg
     1201 ccgagcgctt cgcgatgatc cgtgagcaga tcgaggtctt cccgcgcgtc cctctcctgc
     1261 ggacgtccgc gaagaccggc gccggggtcg ggaaggtggt gccggccctc ctgcaggtgc
     1321 acggggagtg gacgcggcgg gtgccgaccg ccgaggtgaa tcgcgtgctc catcgtgccc
     1381 aggccgagca cccaccccca cgggggggcg ggcggatccg ctacggcacg caggtcgcgt
     1441 cgggcccgcc gcgcttcgtc gtgttcgcca ccgggtcgcg gcagcaggtg tcctaccggc
     1501 ggtacctcga gaaccggttc cgggaggcct tcgggttcga tggcgtgccg atccggatct
     1561 ccttccgggg ccgggaccgg tcccggtccg gaggggcgcg acgcgcggcc ggggagtagc
     1621 atcgagcgat gcgcacgagg ggatcgcgag catgaccgga catcgcgtgc cgtcgggccg
     1681 cgagcacctg gccgccctgg cccggcccct cgcggcgctc gaaggcgacg tcgcccggat
     1741 cgaggggtgg ggggccgacc tcgccgagat cctgctggcc ggggggcgtc tgctcgccgc
     1801 cggcaacggc gggagcgcgg cgcaggcaca gcacctcacg agcgagctcg tcggccgcta
     1861 cggcgtcgat cgcccggccc tgagcgcgat cgcgctgcac gccgagacgt cgagcctcac
     1921 ggcgatcgcg aacgacttcg gtctcgagga ggccttcgct cgccaggtcc gcgcgcacgg
     1981 ccgtcctggc gacgtcctcg tggcgctcag cacgtcgggg cggagcggga acgtcgtcgc
     2041 cgccgtcgac gaggcgcgac gggggaggat gcgcatctgg gccctgacgg ggccggtgcc
     2101 gaaccctgtg gcggacggcg ccgacgatgc cgtcgtggtc gacgcacccg cgacggccac
     2161 gatccaggag gtccaccagg tggcgatcca catcctctgt gccgcggtgg atgcgaccct
     2221 cgtcgagcgg ggcctgacgc ctcgggtgga ggcctgaggc ggcggcaggc tcgacccgtc
     2281 cgaccctggg acggtctgcg gttccctccg cttcggacgt cgccagggga ggtgcttgct
     2341 cgcttcggtt cggctgacta cggttcggag tgtcggtcga gcaaggggga ggagatggat
     2401 cgaacggctc cgagaagggg ctatgcccgc acgttcggtt ggatctgggg gatcgcctac
     2461 ctggccgtcg cgctcctcga gctgctgacg atgaacaacg gcctgcaggt cggcggcacc
     2521 gagatcctgc ggttcggagc gctccacaac gggatccact gggccacggg gatcgcgctg
     2581 atcggctcag ccatggcggg ggagggcgcg gcccgcctcg tctgccggat catcggtgtg
     2641 gcgttcgtcg cggtcgtcat ctggaacttc atctcgccgg actcgtacgc gcgcttcgtc
     2701 gggatggggg acgacctccc ggtttcgtac atcctcgtcc acatcatcac ggccctgggc
     2761 gcgctgttcg ccggcttcgc cggaccggcg accgaccggg accgcacgta gtcacccgac
     2821 gcgagcggga cgagaccctc gggcgacgcg agccggtccg ccggtcctgt ggcgcgccag
     2881 gtgggtccag tggctcctgc gtgcggagcc cgaccgggcc gcaccgatcg gcgccgc
//
LOCUS       DATLGG010000142         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_136280, whole genome shotgun sequence.
ACCESSION   DATLGG010000142 DATLGG010000000
VERSION     DATLGG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136280"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..557)
                     /locus_tag="VM638_04130"
     CDS             complement(132..557)
                     /locus_tag="VM638_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM11651.1"
                     /translation="MSDRESEKDKPHVLLVEDYDDAREVCAALLEAQGYRVSTATNGV
                     EAVEKAQEEHPDVVLMDLSLPLMDGVTAIQKLKQQGETKDIPVALMTGHVAGKRSEDA
                     QSSGCDMVIAKPCMPEELESRIKELLAKKPTPATLLKKD"
     gene            complement(557..1438)
                     /locus_tag="VM638_04135"
     CDS             complement(557..1438)
                     /locus_tag="VM638_04135"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HVM11652.1"
                     /translation="MTAQKGSSLNGSSLNGSSLNGSSLNGSSLNGNPLNTTLASVAFA
                     GLKLNGGGTADAAWLVGTELHAKKGSTIYRGVDLKQAELQGTMADGRTVRLRLRSVTA
                     PPTGDDRWRYGVEYRESDNTWWPVCKQDGVGAVDAYALSGTWSYAFGTASGGAKSDDG
                     TKFTFACTNGASALAKCVDLGYKPWTSFAGVNLENHHQACVRLIRADYCGDGTSYTAD
                     GTPVNLYDGLGIQTDDHDWYFEAEWDERGARCFSPLNRSHEAIPCYQDRVEVGCGSQQ
                     FPFGTLLVNETTDDGIL"
     gene            complement(1580..>2360)
                     /locus_tag="VM638_04140"
     CDS             complement(1580..>2360)
                     /locus_tag="VM638_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HVM11653.1"
                     /translation="FLFGTAADEGLREYYKTAPIHPTETGAYFISRHETTWADWIEFL
                     EALSPAERAKRTPSVSSTGFHGGLSLKKEGGSWRLTMQPGAVRLSAAEGEPIRYPKRE
                     RRAVQDWRKMPVAGINADDARAYAAWLASTGRVPNARLCTEREWERAARGADAREYPH
                     GGQLAPDDANHDRTYGQVPESFGPDEVGAHPVSESPFGVHDLSGNVWEWVESSLEPGS
                     AIARGGSYYFSASTARVVNREKPEQTLRDLTVGVRICAGTE"
BASE COUNT          334 a          830 c          807 g          389 t
ORIGIN      
        1 gtcgacaagc tgatcgccgg caccgcgttc cccgaggagg acgcgggcac gatgatcccc
       61 gcgaccgagg acgatctcga ctccctcgac gagcccgacg aggggggcga cgacggcgcc
      121 ttcccgccgg actagtcctt cttgaggagc gtcgccggcg tcggcttctt cgccaggagc
      181 tccttgatac ggctctcgag ctcctcgggc atgcagggct tggcgatgac catgtcgcac
      241 ccggaggact gcgcgtcctc cgagcgcttg cccgccacgt gccccgtcat caaggcgacg
      301 gggatgtcct tcgtctcgcc ctgctgcttg agcttctgga tcgcggtgac cccatccatg
      361 agcggcagcg agaggtccat gagcacgacg tccgggtgct cctcctgcgc cttctcgacc
      421 gcctccacgc cgttggtcgc ggtcgacacc cggtagccct gcgcctcgag gagcgcggcg
      481 cagacctccc gcgcgtcgtc gtagtcctcg acgaggagga cgtgcggctt gtccttctcg
      541 ctctcgcgat cgctcatcag aggatcccgt cgtcggtcgt ctcgttcacg agcaacgtcc
      601 cgaaggggaa ctgctgggag ccgcagccga cctcgacgcg gtcctggtag caggggatcg
      661 cctcatgcga gcgattgagc ggcgagaaac agcgcgcgcc gcgctcgtcc cactccgcct
      721 cgaagtacca gtcgtggtcg tccgtctgga tgccgagacc gtcatagagg ttcacgggcg
      781 tcccgtcggc ggtatacgag gtgccgtcgc cgcagtagtc ggcgcggatg aggcggacac
      841 acgcctgatg atggttctcc aggttcactc cggcgaagga cgtccacggc ttgtaaccga
      901 ggtcgacgca cttcgcgagc gccgacgcgc cgttcgtgca ggcgaaggtg aatttcgtcc
      961 cgtcgtcgct cttggcgccg cccgacgcgg tgccgaaggc gtagctccag gtgccggaga
     1021 gggcgtacgc atcgaccgcg ccgacgccgt cctgcttgca gaccggccac cacgtgttgt
     1081 cggactcgcg gtattcgacg ccgtagcgcc agcggtcgtc accggtcggc ggcgcggtga
     1141 ccgagcggag gcgcagccga accgtccggc cgtcggccat cgtcccctgc agctccgcct
     1201 gcttgaggtc gacgccgcgg tagatcgtgg agcccttctt cgcgtggagc tccgtgccga
     1261 cgagccaggc ggcgtcggcg gtgccgccgc cgttcagctt gaggccggcg aacgcgaccg
     1321 acgcgagggt cgtgttcagc gggttgccgt tgaggctcga cccgttgagc gaggagccgt
     1381 tgagggagga gccgttgagg gacgagccgt tcaggctcga gcccttctgg gccgtcatct
     1441 cgagctccgc ttcgggagcc cggccgcacg cactgaggag gacgacgaga gcaatggata
     1501 gggaacgcat gagcgaccga tttgcgcacg agtttctccc ttgcaagcga gaaaaccgca
     1561 acgtcagcga cggtcgtcgc tattccgttc cggcgcaaat ccggacgccg accgtcaggt
     1621 cgcgcaaggt ttgctccggc ttctcgcggt tcacgacccg ggcggtgctg gcggagaagt
     1681 aatagctgcc gccgcgcgcg atcgcgctgc cgggctcgag cgaggactcg acccactccc
     1741 atacgtttcc ggacaggtcg tgcacgccga aggggctctc cgagaccgga tgcgcgccga
     1801 cctcgtccgg accgaacgac tccggcacct gtccgtaggt cctgtcgtgg ttcgcgtcgt
     1861 cgggggcgag ctgcccgccg tgcgggtact cgcgcgcatc ggccccgcgg gccgcccgct
     1921 cccactcccg ctccgtgcag agccgcgcgt tcggcacccg tccggtcgag gcgagccacg
     1981 ccgcgtaggc gcgcgcgtcg tccgcgttga tccccgcgac cggcatcttc cgccagtcct
     2041 gcaccgcgcg ccgctcgcgc ttcgggtagc ggatcggctc cccttccgcc gcggagagcc
     2101 ggacggcgcc gggctgcatc gtgagccgcc agctcccgcc ctccttcttc aaggagaggc
     2161 cgccgtggaa tccggtggag gagacgctcg gcgtgcgctt cgcgcgctcc gccggcgaca
     2221 gcgcctcgag gaactcgatc cagtccgccc acgtcgtctc gtggcgcgag atgaagtacg
     2281 cacccgtctc cgtcgggtgg atgggcgccg tcttgtagta ctcgcgcaag ccctcgtccg
     2341 ccgccgtccc gaagaggaag
//
LOCUS       DATLGG010000143         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_100718, whole genome shotgun sequence.
ACCESSION   DATLGG010000143 DATLGG010000000
VERSION     DATLGG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100718"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VM638_04145"
     CDS             complement(<1..516)
                     /locus_tag="VM638_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HVM11654.1"
                     /translation="MSELRSVVEQYRGETLSGLPDARLEEDFSELHRACELLEAERLR
                     RLRELDRRGVGAADGHLSTAGWLAARFRVAWGTAKQQVKLARALEEMPETRKAVEEGE
                     LSLSAARVLVRARDADPEAFRGSEGALVEAARVHSMGDLRKIAAYWQQRVERQAGMEG
                     EERLREQRRLYA"
     gene            complement(544..1191)
                     /locus_tag="VM638_04150"
     CDS             complement(544..1191)
                     /locus_tag="VM638_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11655.1"
                     /translation="MGSTVLLGAATVTWSLYHLIAPIVPPAFLAVGDFGEPLMIMAAG
                     SLLAFATFELRSPFPAWNDRFGPTLRRGMDLTLGVAMGGLYLWLGTSIHFWPEIVGTI
                     QDHEILWVAMYALLGGALFSFLLERWTAMAVAGGVMAAGLSWGTWFEASIYPVFGFSL
                     PYYVLDPVDEYLAIPLLAGPAPLGRTHRAPEDAPRAHRTTRSRSEPLNVLVSWGC"
     gene            complement(1418..2593)
                     /locus_tag="VM638_04155"
     CDS             complement(1418..2593)
                     /locus_tag="VM638_04155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVM11656.1"
                     /translation="MERAVIIEGARTPIGNFMGTLAEVPAVDLGIAATREALRRANVE
                     PEAVDEVVFGHARQAGNGPNPARQVAYRSGIPQEVPAFTVNMACGSGTKAVETAANDI
                     ALGNAEVVVAGGQENMSRTPYLLDRMRGGYRMGDATVYDGMRRDGFLDPLCGLMMGET
                     AENLARKYDIPRREQDEFALASQRKAEEHRDRRAREIVPVEVPGRKGQTVTFEMDEHP
                     RPETTLETLARLKPVFADDGTVTAGNSSGITDGAAALVVMSESRARAEGREPLARIVA
                     AASAGVDPAYMGIGVVPAVRKVLDKTGLSLPDFDVIELNEAFAAQVLAVDRELKLDHD
                     KLNPSGGGIALGHPIGMSGARIVLATAYAMREQGLALGLATLCISGGMGMAIVLERA"
     gene            complement(2604..>2802)
                     /locus_tag="VM638_04160"
     CDS             complement(2604..>2802)
                     /locus_tag="VM638_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11657.1"
                     /translation="RGDESGFSLPLPPDAASEVRAAASAFATAAPRILACDVPPSEPL
                     DGGADLARLRRLLAGGSGERR"
BASE COUNT          455 a         1039 c          923 g          385 t
ORIGIN      
        1 ggcgtagagg cgtcgctgct cccgcagcct ctcctcaccc tccatccccg cctggcgctc
       61 cacccgctgc tgccagtagg cggcgatctt ccgcaggtcc cccatcgaat gcacgcgtgc
      121 ggcctccacc agggccccct cggagccccg gaaggcctcc gggtcggcat cgcgggcccg
      181 caccagcacc cgggccgccg agagcgagag ctcgccctcc tccacggcct tccgggtctc
      241 gggcatctcc tcgagcgccc gcgcaagctt cacctgctgc ttcgccgtcc cccaggcgac
      301 acggaaccga gcagccagcc accccgcggt ggacagatgc ccgtctgcgg ctcccacgcc
      361 acgccgatcg agctcccgca gccgcctgag ccgctcggcc tccaggagct cacacgcccg
      421 gtgcagctcg gagaagtcct cctccagccg cgcgtcggga agcccggaca gcgtctcccc
      481 ccggtactgc tccacgaccg agcggagctc gctcatgcac ccaccccgaa catgtgttcg
      541 agcctagcac ccccaactga caaggacgtt caaaggctcg ctacgggatc gggtggtgcg
      601 gtgagcgcgc ggcgcgtctt ccggtgcccg atgagtccga ccgagaggag caggccccgc
      661 gagtagggga atcgcgaggt actcatctac tgggtccaag acgtagtacg ggagcgaaaa
      721 accgaagacc gggtagatcg aagcttcgaa ccatgtcccc cacgagagcc cggcggccat
      781 cacaccaccg gcgaccgcca tggcggtcca gcgttccagc aagaacgaga agagggcacc
      841 tcccagcagc gcgtacatcg ccacccacag gatctcgtgg tcctggatcg tccccacgat
      901 ctccggccag aaatgaatgc tagtcccgag ccagaggtag aggcctccca tggcgacgcc
      961 gagtgtgaga tccattcctc gacgcagcgt cggtccgaac cgatcgttcc aggcggggaa
     1021 gggagagcga agctcgaagg tcgcgaaagc gagcaaactg cccgccgcca tgatcatcaa
     1081 gggctcaccg aagtcgccga ccgctaggaa cgcgggaggc acgataggag cgatgagatg
     1141 gtagagagac cacgttacgg ttgccgcgcc gagcaggacc gtggacccca ccgtagccag
     1201 tagtacgcgg gagcggccga gcaatcccaa tatggtccca gccgcgaaga caagaacgat
     1261 cacgatcggt gaaaaccttc caccgtgcca tcccatatgg tgagcggatg ccaacgcagc
     1321 ggcgactgcc aggccgccaa cgaactgaag caggcccgtt ctcgtcccgc ggggagcggc
     1381 tgcgcggtgc gtcaccgcag gttccagggg agccctctca ggcgcgttcg aggacgatcg
     1441 ccatgcccat gccgccgctg atgcagaggg tggcgaggcc gagcgccaga ccctgctcgc
     1501 gcatcgcgta ggcggtcgcg agcacgatcc gggcgccgct catcccgatc gggtggccga
     1561 gcgcgatccc gccgccggag ggattcagct tgtcgtggtc gagcttcagc tcgcggtcga
     1621 ccgcgaggac ctgggcggcg aaggcctcgt tcagctcgat cacgtcgaag tcggggagcg
     1681 agagcccggt cttgtcgagc accttccgca cggccgggac gaccccgatc cccatgtacg
     1741 ccggatcgac accagccgag gccgcggcga cgatccgagc gagcggctcc cgcccctcgg
     1801 cccgggcccg cgactccgac atcacgacga gcgccgcagc gccgtcggtg atcccgctcg
     1861 agttgccggc ggtgacggtc ccgtcgtcgg cgaacaccgg cttcagcctc gcgagcgtct
     1921 cgagggtcgt ctccggccgc ggatgctcgt ccatctcgaa ggtcaccgtc tgacccttcc
     1981 ggcccggaac ctcgaccgga acgatctcgc gcgcccgacg atcccgatgc tcctcggcct
     2041 tccgctggct cgcgagcgcg aactcgtcct gctcccgccg cgggatgtcg tacttccgcg
     2101 cgaggttctc ggcggtctcg cccatcatca gcccgcagag gggatcgagg aagccgtcgc
     2161 ggcgcatccc gtcgtagacc gtcgcgtcgc ccatccggta gccgccgcgc atccggtcca
     2221 gcaggtacgg ggtccggctc atgttctcct ggccgccggc gacgacgacc tcggcgttgc
     2281 cgagcgcgat gtcgttcgcc gccgtctcga cggccttcgt ccccgagccg caggccatgt
     2341 tgaccgtgaa ggccgggacc tcctgcggga tgccggagcg gtacgcgacc tggcgcgccg
     2401 ggttcggccc gttcccagcc tggcgcgcgt gcccgaagac gacctcgtcg acggcctcgg
     2461 gctcgacgtt cgcccgtcgc agggcctccc tcgtcgccgc gatgccgagg tcgaccgccg
     2521 gcacctcggc cagggtgccc atgaagttgc cgatcggcgt gcgcgcgccc tcgatgatga
     2581 ccgcccgctc catggaagcc attctaccgg cgttcgcccg agcccccggc gagcagcctg
     2641 cggaggcgcg cgaggtcggc accgccgtcc aggggttcgg agggcgggac gtcgcacgcg
     2701 aggatccgcg gagcggcggt cgcgaaggcc gaggccgccg cccgcacctc gctcgcggcg
     2761 tccggcggga gcggcaagct gaagcccgac tcgtcgccgc ga
//
LOCUS       DATLGG010000144         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_25912, whole genome shotgun sequence.
ACCESSION   DATLGG010000144 DATLGG010000000
VERSION     DATLGG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25912"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VM638_04165"
     CDS             <1..1081
                     /locus_tag="VM638_04165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HVM11658.1"
                     /translation="HRRVGGRHRARQKGGAKLAAAALIVVAAAGAAYGFRAEIRKLIR
                     KEPAVVATPLPAPSGTPTPATTTAAPEPSPTRGRLVVHGTGDVNLDPDYIPAFRTHGY
                     AHAWSGLGGLFERDHLTIVNLECAASKLGSAIPKDFTFRCDPEALPAAKRAGVEVANL
                     GNNHSGDFGKDALLDSRRNLRRAGIEPVGAGKDERQATRPALFDLEGWRVAVLGFGGI
                     VPEPGWIAGPNHPGMADGDDIAKMTDAVRRAKRQADVVFVTIHWGVELDTEPRPDDIE
                     RAHAMIEAGADAIFGHHAHRLQPMAVYRDRPIFWGLGNFVWPATSEAGATTAVARVVV
                     TPEGRIRGKLLPARIESHGHPVLVR"
     gene            1103..1795
                     /locus_tag="VM638_04170"
     CDS             1103..1795
                     /locus_tag="VM638_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM11659.1"
                     /translation="MAQPSILLVDDDARLREIVSMALRAEGYRVDVAASAEEARSALE
                     RDDPDLLILDVMLPGEDGFELSRHIRTTSPVPILMLTAKTDTVDVVVGLESGADDYVT
                     KPFDTKVLVARIRALLRRARSPDRDLPKTISVGDLTIEPEAATVTKRGEPVHLTKTEL
                     KLLCAMAARPNQVFTREVLLQQVWDYDYFGDSRLVDVHIRRLRGKVEDDPRNPAIIQT
                     VRGLGYKVTGAT"
     gene            1792..>2821
                     /locus_tag="VM638_04175"
     CDS             1792..>2821
                     /locus_tag="VM638_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVM11660.1"
                     /translation="MRRRAPHGLRPRITIAFVGGALGVSALVAGSTYFFADRFLYRQR
                     VDESLRQSYGNVRLAVAYLSRPPARSGSLSEEQQADLAISELIDLLGTRGTADALILR
                     GDQPFSSSFSITPALLPPGLRSAVARGEVGYAFSEDPRRLVFGSSIPGYDLDAFFFYP
                     VADIDQTLLILRNVLIAVSGMAVALSAAVGTRVTRRVTTPVRLVSDAARRVAEGLLET
                     RLPVEARDELGHLAHSFNEMAAALEGRIARERRFVGDVSHELRTPLTTLRTSTDYLLS
                     HAQELPPHLRRATELLAEDLAYLQQLVADLLDISRAESGPVDLALEPVNLADLTREVV
                     SRRTRTDGR"
BASE COUNT          407 a         1036 c          991 g          387 t
ORIGIN      
        1 ccatcggcgc gtgggaggga ggcaccgggc acggcagaag ggcggagcga agctggcggc
       61 cgcggcgctg atcgtcgtcg ccgcggccgg cgccgcctac gggttccgag cagagatccg
      121 gaagctcatc cggaaggagc cggcggtcgt ggccacaccg ctccccgccc cgagcggcac
      181 gcccacgccg gccacgacca cggccgcgcc cgaaccgtcg ccgacgcgtg gccgcctcgt
      241 cgtccacggg acgggcgacg tgaacctcga cccggattac atccccgcct tccgtacgca
      301 cgggtacgca cacgcgtgga gcggcctcgg cggcctcttc gagcgggacc acctcaccat
      361 cgtcaacctc gagtgcgcgg cctccaagct cgggtcggcc atcccgaagg acttcacgtt
      421 ccgctgcgac cctgaggccc tgccggcggc gaagcgagcg ggcgtcgagg tcgcgaacct
      481 cggcaacaac cactccgggg acttcggcaa ggacgcgctc ctcgactcgc gccgcaacct
      541 gcggagggcc gggatcgagc cggtgggggc ggggaaggac gagcgtcaag cgacgcgccc
      601 ggcgctgttc gacctcgagg gttggcgcgt cgcggtcctc gggttcggcg gcatcgtgcc
      661 cgagcccggc tggatcgccg gcccgaacca cccggggatg gccgatggtg acgacatcgc
      721 gaagatgacc gacgcggtcc gccgggcgaa acggcaggcg gacgtcgtct tcgtcacgat
      781 ccactggggg gtcgagctcg acacggagcc gcgccccgac gacatcgagc gcgcgcacgc
      841 gatgatcgag gcgggcgcgg atgcgatctt cggccaccac gcgcaccggc tccagccgat
      901 ggcggtttac cgcgaccgtc cgatcttctg gggactgggg aacttcgtct ggcccgcgac
      961 ctccgaggcg ggcgccacca cggcggtcgc ccgggtcgtg gtcactcccg aggggaggat
     1021 ccgcggcaag ctcctgccgg cgcggatcga gtcgcacggt cacccagtgc tcgtccggta
     1081 gccgctccgg ccataatcag gcgtggccca gccctcgatc ctcctcgtcg acgacgacgc
     1141 gcgcctccgc gagatcgtgt cgatggcgct gcgggccgag gggtaccgcg tggacgtcgc
     1201 ggcctcggca gaggaggcgc ggagcgcgct cgagcgggac gacccggacc tcctgatcct
     1261 cgacgtgatg cttccgggcg aggacggctt cgagctctcg cgccacatcc gcacgacgag
     1321 cccggtcccg atcctgatgc tcaccgccaa gacagacacg gtggacgtcg tcgtcgggct
     1381 cgagtccggg gccgacgact acgtgacgaa gcccttcgac acgaaggtcc tcgtcgcacg
     1441 gatccgtgcg ctcctgcgac gggcacgctc accggaccgg gacctgccga agacgatctc
     1501 ggtcggtgac ctgacgatcg agccggaggc cgccacggtg acgaagcgcg gagaaccggt
     1561 gcacctgacg aagacggagc tcaagctgct gtgcgcgatg gccgcgcgtc cgaaccaggt
     1621 gttcacccgg gaggtcctcc tccagcaggt ctgggactac gactacttcg gggactcccg
     1681 gctcgtcgac gtccacatcc gtcgcctgcg aggcaaggtc gaggacgacc cgcggaaccc
     1741 ggccatcatc cagacggtgc gcgggctcgg ctacaaggtg accggagcga catgaggcgg
     1801 cgggcgccgc acggcctgcg accgaggatc acgatcgcct tcgtcggggg cgccctcggg
     1861 gtgtcggcgc tcgtcgccgg gtccacctac ttcttcgccg atcgcttcct gtaccgccag
     1921 cgcgtcgacg agtccctccg gcagagctac gggaacgtcc gcctcgccgt ggcctacctc
     1981 tcgcggcccc cggcgcgctc cgggagcctg tcggaggagc agcaggcgga cctcgcgatc
     2041 tccgagctga tcgaccttct cgggacgcgc gggaccgccg acgcgctgat cctgcggggc
     2101 gaccagccgt tctcgtcgtc gttctcgatc accccggcgc tcctgccgcc ggggctgcgc
     2161 tccgccgtcg cccgcggcga ggtcggctac gcgttctccg aggacccgcg aagactcgtc
     2221 ttcggctcca gcatcccggg ctacgacctg gatgcgttct tcttctatcc ggtcgcggac
     2281 atcgaccaga ccctcctgat cctccgcaac gtgctcatcg ccgtctcggg gatggcggtc
     2341 gcgctgagcg ccgcggtcgg gacgcgcgtg acccgccgcg tgacgacgcc ggtgcggctc
     2401 gtgagcgacg cggcacgacg ggtcgccgag ggtctcctcg agacgcgtct gcccgtggag
     2461 gcgcgcgacg agctcggcca tctcgcgcat tcgttcaacg agatggccgc cgcgctcgag
     2521 gggcgcatcg caagggagcg ccgcttcgtc ggcgacgtct cgcacgagct ccggaccccg
     2581 ctgacgacgc tgcggacctc gacggactac ctcctctcgc acgcgcagga gctgcccccg
     2641 cacctgcgcc gggccaccga gctcctggcg gaggacctcg cgtacctcca gcagctcgtc
     2701 gcggacctcc tggacatctc acgggccgag tcgggtcccg tcgatctggc gctcgaaccc
     2761 gtgaacctgg cggacctgac gcgcgaggtg gtcagccgcc ggacccggac cgacgggcgg
     2821 g
//
LOCUS       DATLGG010000145         3990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_100754, whole genome shotgun sequence.
ACCESSION   DATLGG010000145 DATLGG010000000
VERSION     DATLGG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100754"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..2085)
                     /locus_tag="VM638_04180"
     CDS             complement(286..2085)
                     /locus_tag="VM638_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM11661.1"
                     /translation="MEKDLLLPYRLRTVRLGVLATWMAIAALLAMAILPERPAFALPP
                     YLITLGVGGLGVLIVSLLPWRRLFERGIGIAVMYAWSVLDIVLITVLLGIIGPDRIEG
                     FVLYGLTTLFFAASYPPAGQVALLLFTCASYLGMLVLTGTDLGAGPLTARLALLGITT
                     FLTCFLWLELMREMRAHHGARADSERRSQLLASVAHAARGISFLESDRVLGAVTDSAV
                     ALGFEAVEICVYDGSAGTYRVAHHRNVPEGYANATHSARRGIVSMVLDRKAPVVLADY
                     TEHPLSEPALRSSGFRAAIATPVWLSGRIQAILSAATKTRRRIGDDEREAFELLGTIA
                     GRALENATLFEQEQRTVERMAELDRLKSEFLSTVSHEIRTPLTVIEGNGLTLEQHWAT
                     LDDRTKLEFIATLNSNARTLDGIITSLLDFSQLEAGKLVARPTEVEFLPLAQSVVARL
                     VKPNPDHLVVVEVDEGLRVVADPLLLDRVIENLLSNALKYSPPGSGIAVTAAIHDGTA
                     SVSVADSGPGIPAEEVARLGERFFRGGDANARSTKGIGLGLALVREVLALHETGLEVA
                     SEVGRGSRFSFRLPLVAPAPVTSNPRSGLVDAG"
     gene            2302..3081
                     /locus_tag="VM638_04185"
     CDS             2302..3081
                     /locus_tag="VM638_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVM11662.1"
                     /translation="MNDENGTDGRAVYEHRWGASGRLVQLRPHLWRELAPLLEGQRVL
                     EIGPGLRPTAPATTAFFVESSPRAATALGAAGGRSVRAGEGPLPFRDASFGGVLALEV
                     LEHVEEDVELLREIRRVLRPGGVLVVSVPVHMSRWSAIDEACGHVRRYEDGELIEKLT
                     VAGFVPERFHERPARGGSGRLAKVGTAIQASTPRLANWWLQSVAFPIHAKWQGLAGRV
                     HWTDPAVPVSARAAGITVLARSAPDASPGEVAPAAADVGRG"
     gene            complement(3078..3260)
                     /locus_tag="VM638_04190"
     CDS             complement(3078..3260)
                     /locus_tag="VM638_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11663.1"
                     /translation="MADEDDRTCANCGTELGPADEACPGCGIPRDEALNRGDRDVDEV
                     TDASMDSFPASDPPAY"
     gene            complement(3327..>3990)
                     /locus_tag="VM638_04195"
     CDS             complement(3327..>3990)
                     /locus_tag="VM638_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HVM11664.1"
                     /translation="LWEVYWNLVAEHGFNPDLDDAWNTGGNNLAVQLVMDGMKFQPCR
                     PGFEDGRDAILAADRALTGGENSCAIWRGFAKRGLGYSARQGSSGSRSDGVQAFDLHP
                     DCQAQAGVSPTSIAATVSEGGTTTETLRIANLAQRGGQTLGWSIAEAPSSCAAPADVA
                     WLAVSPSSGDTAPGRSTEVDVRLAAGGAPAGSRSTGRICVLSNDAGSSSIAIPVSLEV
                     TG"
BASE COUNT          569 a         1396 c         1475 g          550 t
ORIGIN      
        1 gcgcggcgac gacgcggcga acgtcctcgc cgggggacgc ggccgagacc ggctctccgg
       61 gcgcggcggc gccgacctcc tcgacggggg cagggggtcg gacgtgtgcc gaggcggagc
      121 gggggcggac cgcttccgaa gctgcgagcg gaagaagcag tagcgcccca gggctcgatc
      181 cgacctcagg tgccgcggcc ggtcttgcgc tgaacctcgt cgatccgcct ggaggggtcg
      241 ggcttggtga ggctctcgtc gaactcctga tcggtcggag cggtcctacc cagcgtccac
      301 gaggcccgaa cgtgggttcg aggtgacggg agccggggct acgagcggca ggcggaagga
      361 gaaacgggag ccgcggccga cctcgctcgc gacctcgagc ccggtctcat gcagggcgag
      421 cacctcgcgc acgagcgcca gtccgagtcc gatccccttg gtggagcggg cgttcgcgtc
      481 tccgccgcgg aagaaccgct cgccgaggcg cgcgacctcc tccgcgggta tcccggggcc
      541 gctgtccgcc acgctgaccg acgccgtccc gtcgtggatc gccgcggtga cggcgatccc
      601 tgagccaggc gggctgtact tgagcgcgtt cgacaggagg ttctcgatca cgcggtcgag
      661 gagcagcgga tcggcgacga cgcgcaagcc ctcgtccacc tccaccacca cgaggtggtc
      721 ggggttcggc ttcacgagcc tggccacgac gctttgggcc agcggcagga actccacctc
      781 ggtggggcgc gcgacgagct tccccgcctc gagctgggag aagtcgagca gcgaggtgat
      841 gatcccatcg agcgtgcggg cgttcgagtt cagggtcgcg atgaactcga gcttcgtccg
      901 gtcgtcgagg gtggcccagt gctgctcgag cgtcaggccg ttgccctcga tgacggtgag
      961 cggcgtccgg atctcgtgcg agaccgtcga gaggaactcc gacttgaggc ggtcgagctc
     1021 ggccatgcgt tcgaccgtgc gctgctcctg ctcgaacagc gtcgcgttct cgagcgcgcg
     1081 tccggcgatc gtccccagca gctcgaaggc ctcgcgctcg tcgtcaccga tgcgtcgccg
     1141 cgtcttcgtc gcggcgctga ggatcgcctg gatcctgccg ctgagccaga ccggcgtggc
     1201 gatcgccgcg cggaaccccg agcttcgcag cgccggctcg ctcagcggat gctccgtgta
     1261 atccgccagg acgaccggag ccttccggtc gaggaccatc gacacgatgc cccggcgcgc
     1321 ggagtgcgtg gcgttcgcgt agccctcggg gacgttgcgg tggtgcgcca cgcggtaggt
     1381 tcccgcggac ccgtcgtaga cgcagatctc gaccgcctcg aagccgagcg cgacggccga
     1441 gtcggtgacg gcgccgagga cgcgatccga ctcgaggaag ctgatccccc gcgcggcgtg
     1501 agcgacgctg gcgaggagct gcgagcgtcg ctcggagtcc gcccgggcgc cgtggtgggc
     1561 gcgcatctcg cgcatcagct ccagccacag gaagcacgtg aggaaggtcg tgatgccgag
     1621 gagcgcgagg cgcgccgtga gtgggcccgc accgaggtcc gtgccggtca gcacgagcat
     1681 ccccaggtac gacgcgcacg tgaacagcag cagcgcgacc tggcccgccg gagggtagga
     1741 agccgcgaag aacagcgtcg tcagcccgta gaggacgaag ccttcgatcc gatccggtcc
     1801 gatgatcccg agcaggacgg tgatcaggac gatgtcgagc accgaccagg cgtacatcac
     1861 cgcgatcccg atgccgcgct cgaacagccg cctccacggc aggagggaga cgatcagcac
     1921 gccgagaccc ccgaccccca gcgtgatgag gtacggcggg agggcgaacg ccgggcgctc
     1981 aggcaggatg gccatggcca gcagggccgc gatcgccatc cacgtcgcca ggacgccgag
     2041 gcgcacggtc cgcaggcggt agggcagcag gaggtccttc tccatcttcc ggttttcggc
     2101 agggacgacg ctcggctgga tgacaccgag gcatcagaac gcgcatggcc tcgtcggcgc
     2161 ccccgggtcc cccatctggg cgatgaccgg atccgccagc aggtcgcgtg gcgcggcaag
     2221 cagcgcgctg cggcatactc cgagttcgat ggacgggggg cgacgtgcgg cgcgccggag
     2281 cgcggggtag gtaggccgac ggtgaacgac gagaacggca cggacggtcg cgcggtctat
     2341 gaacatcgat ggggcgcttc cgggcggctc gtccagctgc ggccgcacct ctggcgagag
     2401 ctcgccccac tcctcgaggg ccaacgggtg ctcgagatcg gacccggcct gcgaccgacg
     2461 gctcccgcga cgacggcgtt cttcgtcgag agctcacccc gcgcggcgac ggcactcggg
     2521 gcggccggcg gacggagcgt gcgggccggg gagggaccgc tccccttccg cgatgcgagc
     2581 ttcggcggcg tcctcgcgct cgaggtcctc gagcacgtcg aggaggacgt cgaactcctg
     2641 cgtgagatcc gacgggtcct gcgtccggga ggggtcctcg tcgtgtcggt cccggtccac
     2701 atgtcccgct ggtcggcgat cgacgaggcc tgcggtcacg tccgccgcta cgaggacggc
     2761 gagctgatcg agaagctgac cgtggcggga ttcgtgcccg agcgcttcca tgagcggccg
     2821 gctcggggcg gcagcggccg cctcgcgaag gtcgggaccg cgatccaggc gagcacgccg
     2881 cggctcgcga actggtggct ccagtccgtc gccttcccga tccacgcgaa gtggcagggt
     2941 ctcgcaggac gggtgcattg gacggacccg gccgtcccgg tctcagcgcg agcggcgggg
     3001 atcaccgtcc tcgccagatc ggcgccggat gcctcgccgg gcgaggtggc tccggcggcc
     3061 gccgacgtcg gtcgcggcta gtaggccggc gggtcgctcg ccgggaacga gtccatcgac
     3121 gcgtcggtca cctcgtcgac gtcccggtcg ccccggttca gcgcctcgtc tcgcgggatg
     3181 ccgcaaccgg ggcaggcctc atcggccggc ccgagctccg tcccgcagtt cgcgcacgtg
     3241 cgatcgtctt catctgccat gacccatccc tacccggccg cggccaggcc tccacctcgg
     3301 ccgcgcggat ggagcgcagc gccacctcag cccgtgacct ccaggctgac cgggatcgcg
     3361 atgctcgagg accccgcgtc gttgctgagg acgcagatcc ggcccgtcga cctggatccc
     3421 gccggggctc cccccgcggc gagccggacg tcgacctcgg tggaacgacc cggggccgtg
     3481 tcccccgagg acggactcac ggcgagccac gccacgtccg ccggggcggc gcagctcgag
     3541 ggggcctccg cgatggacca gccgagggtc tgccccccgc gctgcgccag attggcgatc
     3601 cggagcgtct ccgtggtcgt gccgccttcg gacaccgtcg cggcgatgct ggtgggtgag
     3661 acccccgcct gcgcctggca gtccgggtgg aggtcgaagg cctgcacgcc atcgctccgg
     3721 ctcccgctgc tgccctggcg agcgctgtag ccgaggccgc gcttggcgaa cccgcgccag
     3781 atcgcgcacg agttctcgcc gccggtcagc gcgcggtcgg cggcgaggat cgcgtcgcgg
     3841 ccgtcctcga agccgggccg gcagggctgg aacttcatgc cgtccatgac cagctgcacc
     3901 gcgaggttgt tgccaccggt gttccacgcg tcgtcgaggt cggggttgaa gccgtgctcg
     3961 gcgacgaggt tccagtagac ctcccacagc
//
LOCUS       DATLGG010000146         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_41888, whole genome shotgun sequence.
ACCESSION   DATLGG010000146 DATLGG010000000
VERSION     DATLGG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41888"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..772)
                     /gene="rdgB"
                     /locus_tag="VM638_04200"
     CDS             complement(149..772)
                     /gene="rdgB"
                     /locus_tag="VM638_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HVM11665.1"
                     /translation="MTFPERVVLATRNRGKIREILAICADWPVTWLTQEDLDWPEVEE
                     TGDTYEENARLKARAVAAATGLPALADDSGIEVDALGGGPGPRSARYAGADASDEQNL
                     RALLRAVAGVPPGGRTARYRAVDVLALPDGPEWSAEGVCEGSLRSRPTGTGGFGYDPI
                     FEPAGWERTMAEMTAEEKHRISHRGKAFRQLRDLLAQAGNPPGASNT"
     gene            complement(769..1488)
                     /gene="rph"
                     /locus_tag="VM638_04205"
     CDS             complement(769..1488)
                     /gene="rph"
                     /locus_tag="VM638_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HVM11666.1"
                     /translation="MTRVDGRAPGELRPVRYRTGYLRWAEGSVLFEMGETRVLCAASY
                     EPSVPRFLQGKGQGWVTGEYSMLPRSTQERTPREAARGRPSGRTQEIQRLIGRSLRAA
                     TDLRALGEVTIWVDCDVLQADGGTRTASITGGYIALALAIRSLKEQGIVRGEPLNDPV
                     AAISAGIVEGTPFLDLTYEEDARAEVDMNVVMTGSGNLVEIQGTAEGAAFSRGELDEM
                     ISLATSGIERLTAIQREAAQA"
     gene            complement(1485..2240)
                     /locus_tag="VM638_04210"
     CDS             complement(1485..2240)
                     /locus_tag="VM638_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVM11667.1"
                     /translation="MEVTVLGACGTWPGPGRACSGYLVRHDGFDVWVDLGSGTLANAQ
                     EHVDLVEIDAVIVSHSHADHFVDLLPYFYARHYALGKPMGTPLFWPPGVYERAGVLLS
                     EETLQELSASFLIHEVEPGTAFDLGPFRVTTEPMAHPVPTLGVRFEVEGRALAYTADS
                     GPAPEVPKLARGADVLLADATWQAGTGEFPPGIHMTDREAGEAAREAEVGRLVLTHIW
                     PSLDPNRSREQAAEAFGAEVEVAQEGMTIEVGP"
     gene            complement(2231..>2701)
                     /locus_tag="VM638_04215"
     CDS             complement(2231..>2701)
                     /locus_tag="VM638_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HVM11668.1"
                     /translation="GTEATVRSGAYDRAARRANPDVDLVSASCPAFVPAVEAGDTTSD
                     ELLEAARGYLAPLVSAGIDTLILGCTHYPLLSGLLRIVMGPDVVLVSSAEETARDVYE
                     TLHRDGLLRDGGGAPAHEFLCSGDPDSFLALGGRFLGPEMASVRSAGVREAAWR"
BASE COUNT          357 a         1018 c          951 g          375 t
ORIGIN      
        1 ccggagggtt gagtgcccgt tctgttcccg gttcgcccgt tgctggcgcg atccggagcc
       61 gtgcgaccgc tctccgatcc gccttccgcc tggcaacctt accggggagt cctggtatgg
      121 atatcggcct cagcggccga cccttgactc acgtattcga cgctcccggc gggttccctg
      181 cctgggcgag gagatcccgc agctgccgga aggccttgcc acgatggctg atgcgatgct
      241 tctcctcggc ggtcatctcg gccatcgtcc gctcccatcc ggcgggctcg aagatcgggt
      301 cgtagccgaa cccgcccgtg ccggtcggcc ggctccggag ggacccctcg cagaccccct
      361 cggcggacca ctcaggcccg tccgggagcg cgaggacgtc gaccgcgcgg tagcgggcgg
      421 tccgtccgcc cggcggcacc ccggcgacgg cccgcaggag cgcgcggagg ttctgctcgt
      481 ccgatgcatc cgccccggcg tagcgtgcgg atcgagggcc cggaccgccg ccgagcgcgt
      541 cgacctcgat cccggagtcg tcggccagcg ccggcaggcc cgtcgcggcc gcgaccgccc
      601 gcgccttcag ccgcgcgttc tcctcgtacg tatccccggt ctcctcgacc tcgggccagt
      661 cgaggtcctc ctgcgtcagc cacgtgaccg gccagtcggc gcagatcgca aggatctccc
      721 ggatcttgcc gcggttgcgc gtcgcgagga cgacccgctc tgggaacgtc acgcctgcgc
      781 ggcctcgcgc tggatcgccg tcagccgctc gatgccggac gtcgcgaggg agatcatctc
      841 gtcgagctcg ccgcgcgaga aggccgcgcc ctccgccgtc ccctggatct cgacgaggtt
      901 gccggagccg gtcatcacga cgttcatgtc gacctccgcc ctcgcgtcct cctcgtaggt
      961 gagatcgagg aacggcgtcc cctcgacgat gccggcggag atcgccgcga cggggtcgtt
     1021 cagcggctct ccacgcacga tcccctgctc cttgagggac cggatcgcga gggcgagcgc
     1081 gatgtagccc ccggtgatcg aggcggtgcg cgtccccccg tcggcctgca ggacgtcgca
     1141 gtcgacccag atcgtcacct cgccgagggc ccgaagatcg gtcgccgccc gcagcgagcg
     1201 gccgatcagg cgctggatct cctgggtccg gcccgacggg cgcccccgtg ccgcttcgcg
     1261 cggcgtccgc tcctgggtcg agcgcggaag catcgagtac tcgccggtca cccacccctg
     1321 tcccttgccc tggaggaacc gcgggaccga cggctcgtag gaggccgcgc acaggacccg
     1381 cgtctccccc atctcgaaca gcaccgaccc ctccgcccac cgcaggtagc cggtgcgata
     1441 ccggaccggg cgcagttcgc cgggcgcccg gccgtcgacc cgcgtcacgg gccgacctcg
     1501 atcgtcatcc cctcctgcgc gacctcgacc tcggcgccga aggcctccgc cgcctgctcg
     1561 cgcgagcggt tcgggtcgag ggacggccag atgtgggtca gcacgagccg tccgacctcc
     1621 gcttcgcggg cggcctcgcc ggcctcgcgg tcggtcatgt ggatccccgg cgggaactcg
     1681 ccggttccgg cctgccaggt cgcgtccgcg agcaggacgt ccgcgccgcg ggcgagcttc
     1741 ggcacctcgg gggccgggcc cgagtccgcg gtgtaggcga gcgcccgccc ctcgacctcg
     1801 aaccgcaccc ccagggtggg gacgggatgc gccatcggct cggtcgtgac gcggaacgga
     1861 ccgaggtcga aggccgtccc cggctcgacc tcgtggatca ggaagctcgc gctcagctcc
     1921 tggagggtct cctcggagag gaggaccccc gcccgctcgt agacccccgg gggccagaac
     1981 agcggcgttc ccatcggctt cccgagcgcg tagtggcgcg cgtagaagta cgggagcagg
     2041 tcgacgaagt gatcggcgtg cgagtgcgag acgatgaccg cgtcgatctc gacgaggtcg
     2101 acgtgctcct gggcgttcgc gagcgtgccc gatccgaggt cgacccagac gtcgaacccg
     2161 tcgtggcgga ccaggtagcc gctgcacgcg cgccccgggc ccggccaggt cccgcaggcg
     2221 ccgaggaccg ttacctccac gcagcctccc gcaccccggc ggagcggacg ctcgccatct
     2281 cggggccgag gaagcgaccg ccgagcgcga ggaacgagtc ggggtcgccg gagcagagga
     2341 actcgtgcgc gggcgctcct ccaccgtcgc gcaggaggcc gtcgcggtgg agcgtctcgt
     2401 agacgtccct ggcggtctcc tcggcggagg agacgagcac gacgtccggc cccatcacga
     2461 tgcgcagcaa accagacagc agtgggtagt gcgtgcaccc gaggatcagg gtgtcgatcc
     2521 cggccgagac gagcggagcg aggtagccgc gggccgcctc cagcagctcg tcgctcgtgg
     2581 tgtcgccggc ctcgaccgcc ggaacgaacg cggggcagga cgccgagacc aggtccacgt
     2641 ccgggttcgc ccgtcgagcc gcgcggtcgt aggccccgct gcgaacggtg gcctcggtcc
     2701 c
//
LOCUS       DATLGG010000147         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_41982, whole genome shotgun sequence.
ACCESSION   DATLGG010000147 DATLGG010000000
VERSION     DATLGG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41982"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VM638_04220"
     CDS             <1..766
                     /locus_tag="VM638_04220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVM11669.1"
                     /translation="RTVRDGLAVYMAGGTSHPDWRRRGLGRAILRATEDHLRDRATAF
                     EDPEGRVLGAWIGEKESGAGALLAGEGYTPVRYYFSMIRQELQEIPHAPLPDGIEIRP
                     VEPDQHRAILDAENEAFRDHWGHREMTEEDFEGLFKLPDLDTSLWSVAWDGDEVAGSV
                     QTFIWKAENEALGEKRGWLERISVRRPWRRRGLAKAIIADALRRLRAAGMEVAMLGVD
                     ADNPTGALQLYEALGFQTKDRGIAYRKAWQPGGRLV"
     gene            complement(778..1416)
                     /locus_tag="VM638_04225"
     CDS             complement(778..1416)
                     /locus_tag="VM638_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVM11670.1"
                     /translation="MELISRVVGPISTNVYVLADERSREAIAIDTATPSLRWIADELA
                     AREWILKLIVSTHGHWDHIGDNAVVADHTGAPIAVHPLDRDRLTSPQPLWAPFEIPPS
                     VPAVELAEGGVIRFGELRLSVLHTPGHTEGSVCLLSADNGLLFSGDTLFAGGWGRVDL
                     PGGDPAAMVESLGRLATLDEPVQVLPGHGNATTVGRERPWMELVAKEGRLFA"
     gene            complement(1464..1826)
                     /locus_tag="VM638_04230"
     CDS             complement(1464..1826)
                     /locus_tag="VM638_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11671.1"
                     /translation="MVLLGCLAAACGPISPPAPAGTLALVLVAGPTCPVMSVPPDPGC
                     APRPVTGQVIVIVEGDREVARGTSDANGMLTFSLPYGRYIVHSISDGSLPMAPADQVV
                     DVGPVPTELVLEFDTGIR"
     gene            complement(1897..>2498)
                     /locus_tag="VM638_04235"
     CDS             complement(1897..>2498)
                     /locus_tag="VM638_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11672.1"
                     /translation="EVVALDPSPAMLDALAEQQAVHGIANVRIVRGHWPPEPGSDPDQ
                     ALGQAPHADVALIAHVGYDVEEIGPFLDAMDKAARRLCVAILMERPPASAADPFWQRV
                     HGVERIPLPALSDFVALLRARGREPEVTSFERRARHFRSREELVKFLRRQLWIADGSA
                     AEERFQAALAELAEETADGWSIAGVRPSLVGLVTWTPRG"
BASE COUNT          390 a          883 c          900 g          325 t
ORIGIN      
        1 ccgcacggtc cgtgacgggc tcgcggtcta catggccggc gggacgagcc accccgactg
       61 gcggcgtcgc ggcctgggcc gggcgatcct gcgcgcgacc gaggaccacc tgcgggaccg
      121 ggcgaccgcc ttcgaggacc ccgagggccg ggtcctcgga gcgtggatcg gcgagaagga
      181 gtcgggagcg ggagcactcc tggccggcga gggctacacc cccgtgcgct actacttctc
      241 gatgattcgc caggagctgc aggagatccc gcatgccccg ctcccggacg gcatcgagat
      301 ccgtcccgtc gagccggacc agcatcgcgc gatcctggac gcggagaacg aggccttccg
      361 cgaccactgg gggcaccgcg agatgacgga ggaggacttc gagggcctgt tcaagctgcc
      421 ggacctcgac acctccctgt ggtccgtcgc gtgggacggt gacgaggtcg ccgggtccgt
      481 gcagacgttc atctggaagg cggagaacga agcgctcgga gagaagcgag gctggctcga
      541 gcggatctcc gtacgccgtc cgtggcgccg gcgtgggctg gcgaaggcga tcatcgccga
      601 cgccctccgg cgtctccgcg cggcgggcat ggaggtggcc atgctcggtg tcgacgcgga
      661 caacccgacc ggcgcgctcc agctgtacga ggcgctcggc ttccagacga aggatcgcgg
      721 catcgcctac cgcaaggcct ggcagccggg tggccggctc gtctgaccgc cgctgactca
      781 ggcgaacagt cgcccctcct tcgctaccag ctccatccac gggcgctcgc gcccgacggt
      841 ggtcgcgttc ccatggccgg gcagcacctg caccggctcg tccagcgtgg ccagccgacc
      901 gagcgactcg accatcgccg ccggatcgcc accgggcagg tccactcttc cccatccgcc
      961 tgcgaacagc gtgtcgccgc tgaacagcag gccattgtcg gccgacagca gacagacgga
     1021 gccctcggtg tggccgggcg tgtggaggac cgagaggcgc agctcgccga accggatgac
     1081 cccgccctcc gcgagctcga cggccgggac ggaagggggg atctcgaacg gcgcccagag
     1141 gggctggggg gaggtgagcc gatcccggtc caggggatgg acggcgatcg gggcgccggt
     1201 gtgatcggcc accacggcgt tgtccccgat gtggtcccag tgcccgtgcg tggagacgat
     1261 cagcttgagt atccactccc tggccgcgag ctcgtcggcg atccagcgca gggagggcgt
     1321 cgcggtgtcg atggcaatcg cctcacgtga acgctcatcc gcgagaacgt agacgttcgt
     1381 cgagatcgga ccgacgacgc ggctgatgag ctccacgcga gcgattgtcg caggccgcgg
     1441 cgccccaccc tttggcgcgg gactcagcgg atcccggtgt cgaactccag cacgagctcc
     1501 gtcggcaccg ggccgacgtc gactacctgg tcggcggggg ccatcggcag gctgccgtcg
     1561 gagatcgaat ggacgatgta gcggccgtac gggagggaga acgtcagcat gccgttcgca
     1621 tcgctcgtgc cgcgggcgac ctcgcgatcg ccctcgacga tgacgatgac ctgcccggtc
     1681 accgggcgcg gcgcgcatcc cgggtccggt ggaacgctca tcaccggaca ggtgggtccg
     1741 gcaacgagga cgagcgccag ggttcccgca ggcgcgggcg gcgagatcgg accgcaggcg
     1801 gccgccaggc agccgaggag caccatcgca gtgaggagcc gccgggtcac aggcatcgaa
     1861 acccggacgt cgcgcgctgc catccggttg cctcgctcac ccgcgcggcg tccaggtcac
     1921 gagccccacc agcgatgggc gaacgcccgc aatggaccac ccgtccgccg tctcctcggc
     1981 cagctccgcg agggccgcct ggaagcgctc ctccgcggcc gaaccgtcgg cgatccagag
     2041 ctgccgccgg aggaacttga cgagctcctc gcgtgatcgg aagtgccggg ctctgcgctc
     2101 gaacgacgta acctccggct cccgaccacg cgcccggagg agggcaacga agtccgacag
     2161 cgccgggagt ggaatgcgct ccacgccatg gacccgctgc cagaagggat cggccgcgga
     2221 cgctggtgga cgctccatca ggatcgccac gcagaggcgg cgggcggcct tgtccatggc
     2281 gtcgaggaac ggcccgatct cctccacgtc gtagcccaca tgggcaatca gcgcgacgtc
     2341 ggcgtgcggg gcctggccca gcgcctggtc gggatcgctc cccggctccg gaggccagtg
     2401 gccgcgcacg atccgcacgt tcgcgatccc gtggaccgcc tgctgctcgg ccagcgcgtc
     2461 gagcatcgcc ggcgacgggt caagcgccac cacctcgc
//
LOCUS       DATLGG010000148         2082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_26099, whole genome shotgun sequence.
ACCESSION   DATLGG010000148 DATLGG010000000
VERSION     DATLGG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26099"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /gene="fabG"
                     /locus_tag="VM638_04240"
     CDS             complement(<1..96)
                     /gene="fabG"
                     /locus_tag="VM638_04240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008608296.1"
                     /note="catalyzes the first of the two reduction steps in
                     the elongation cycle of fatty acid synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HVM11673.1"
                     /translation="MPDFASLEGKTALVTGASKGIGRAIAKELAAA"
     gene            complement(89..958)
                     /locus_tag="VM638_04245"
     CDS             complement(89..958)
                     /locus_tag="VM638_04245"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HVM11674.1"
                     /translation="MGREVAEAVPEAMEVYEAGSDASGLDLKRICFHDPVENLLDTRI
                     QQPALVATSLALDAAIRARGIEPDFVVGHSVGEFAALGAAGSLGAPEAIALVRDRGIA
                     MAEAARERPGSMAAILGLADEVVESLCHRIANVWPANYNCPGQIVVSGEDPAVGEVCA
                     EAEREGARRAVRLRVSGAFHSPLVARAADKLRPAVDRVHFHDPRHAFMSTVTAKLEDA
                     HRYRELLIEQLTAPVKFTQAARELVEKGVTVFVEVGPGNVLSGLLKRIDRGVRTVSVS
                     DLEGLRQLEETLA"
     gene            962..1918
                     /locus_tag="VM638_04250"
                     /pseudo
     CDS             962..1918
                     /locus_tag="VM638_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
BASE COUNT          307 a          718 c          763 g          294 t
ORIGIN      
        1 ggcggcggcg agctccttcg cgatcgcgcg cccgatgccc ttcgacgcgc cggtgacgag
       61 cgctgtcttg ccctcgagcg aggcgaagtc aggcaagcgt ctcctccagc tggcgcaggc
      121 cctcgaggtc gctgacggag acggtgcgga ctccgcgatc gatcctcttc agtaaaccgc
      181 tcaggacgtt gccgggcccg acctcgacga acaccgtgac gcccttctcg acgagctcgc
      241 gcgccgcctg cgtgaacttc acgggtgccg tgagctgctc gatcaggagc tcccggtagc
      301 ggtgcgcgtc ctccagcttc gccgtcaccg tcgacatgaa cgcgtgccgc gggtcgtgga
      361 agtggacgcg gtcgacggcg gggcgcagct tgtccgccgc gcgcgcgacg agcggcgagt
      421 ggaacgcacc gctcacgcgc agacgcaccg cgcgtcgcgc gccctcgcgc tcggcctcgg
      481 cgcagacctc gcccaccgcc ggatcctcgc cggagacgac gatctgcccg gggcagttgt
      541 agttcgccgg ccagacgttc gcgatccggt ggcagaggct ctcgaccacc tcgtctgcga
      601 ggccgaggat cgccgccatc gagcccggcc gctcacgggc ggcctcggcc atcgcgatgc
      661 cgcggtcgcg cacgagcgcg atcgcctcgg gagccccgag cgacccggct gcgccgagcg
      721 ctgcgaattc gcccaccgag tgcccgacga cgaagtccgg ctcgatcccg cgcgcccgga
      781 tcgcggcgtc gagcgcgagg ctcgtggcga cgagcgccgg ctgctggatc cgcgtgtcga
      841 gcaggttctc gacggggtcg tggaagcaga tccgcttgag gtcgaggccg gaggcgtcgc
      901 tcccggcctc gtagacctcc atcgcctcgg gcaccgcctc cgccacctcg cgccccatcc
      961 cggcctcggg acgcacgttc cggacacggt gttcacgaac accgacctcg agcagtacgt
     1021 cgacacgacg gacgagtgga tccaggagcg gacggggatc agggagcgcc gcttcgcgaa
     1081 gcaggaggag gcgctgaccg acctcgcccg tccggcggcg ctgcaggcgc tcgagcgcgc
     1141 cggtgccgag cctgcgagcg tcgatctgat catcgtcgcg acggtgacgc ccgacatgat
     1201 gttcccgacg agctcggcgc tcctcgccga ccagctcggg atgcccgacg cggccgccta
     1261 cgacctgctc gccggctgca cgggcttcat gtacggactc gctcaggcgt acgggatgat
     1321 cgcagcgggc ctgtcgaagc gggcgctcgt cgtcggcggc gacgtgctct cgaagatcct
     1381 cgactggagc gaccgctcga cgctcgtcct cttcggcgac ggtgcggggg ccgtcgtgct
     1441 cgagcaggtc gaggagggag ggttcctcgg gttcgagctc ggcgcggacg gcggcggcgg
     1501 cgagcacctc tggctgccgg gcagcggttc ccgccacttc gaggacccga cgaggttcgt
     1561 gaagatgaac gggcgggagg tcttcaagtt cgcgacgcgg gtgatggtgt cgtcggcgac
     1621 gaaggtgctc gacgagtgcg gccggaccgt cgacgacgtc gacgtctacg tgccgcacca
     1681 ggcgaacaag cggatcatcg accacgcagc ggggaagctg gggatcccgg cggagaagac
     1741 ggtcgtgaac gtcgaccgct acggcaacac gtcgtcgggg tcgatcccgc tcgccctcgc
     1801 cgacgcgaag acggacggga ggctccgtcc gggcgagctc gtactgatga caggcatggg
     1861 cgccggcctc acgtggggct cggcgctgat cgagtggacg gagggaggaa cggcgtgacg
     1921 aagatcgcct tcatgttccc gggccagggt tcgtacgagg ccgggatggg gcgcgaggtg
     1981 gcggaggcgg tgcccgaggc gatggaggtc tacgaggccg ggagcgacgc ctccggcctc
     2041 gacctcaagc ggatctgctt ccacgacccc gtcgagaacc tg
//
LOCUS       DATLGG010000149         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_136662, whole genome shotgun sequence.
ACCESSION   DATLGG010000149 DATLGG010000000
VERSION     DATLGG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136662"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..788
                     /locus_tag="VM638_04255"
     CDS             162..788
                     /locus_tag="VM638_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HVM11675.1"
                     /translation="MFEDRSDAGRRLAEALPEEESSSVVIGIPRGGVAVGAEVARLRG
                     WDLDVVVPRKVRAPQNPELGIGAIAPGVRLLDEGMIATLAVDPDYLVREVADQEREIA
                     RRLAAYRGDRPPLDLAGRTGIVIDDGVATGSTAVAAVRSVRAAGARRVVVAVPVAPAE
                     AVERLRREADGVVCLITPARFHAVGEWYRRFPQVQDPEVVAHLDAAWS"
     gene            779..1060
                     /locus_tag="VM638_04260"
     CDS             779..1060
                     /locus_tag="VM638_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11676.1"
                     /translation="MVVIEIALGGLCLAVGVWSAVGSVRALSAEEPGPDRRVLVLHEW
                     AKAGFWLTLGAFLLGYLVLDDPREIRGFALVPIVLAGIRFLAAVRLARR"
     gene            1163..>2017
                     /locus_tag="VM638_04265"
     CDS             1163..>2017
                     /locus_tag="VM638_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVM11677.1"
                     /translation="MKPTVSLESMLGAQRGDPPAALIPRYLAEARRQLGMEVAYVAEF
                     RDGQRIYRFIEGETIPDGVRVGTGDPLEDTFAQRVIEGSVDAVPDVARDPALASLVET
                     RSSRIASFVGVPLRLGVDDIYGILACQSSTPTPSLDDRAVSFLHVFGRLILDQLDKEQ
                     GEWDDLRRQIERVEQALQPGSIGIEFQPVFDIRSDDGRLVGAEALARFELEPQRSPDV
                     WFAEAWTVGRGVDLELASVEAAVAHLGRLPQGTFLAINVAAETVVSPRLPGILAGVPR
                     DRVVLEIPE"
BASE COUNT          257 a          698 c          749 g          313 t
ORIGIN      
        1 gcgacctcct tcgcgacctg cgctgccttg ccgacctccg tctggatcag gatgtacgca
       61 ttcaccaccg tgagcacctc ccgggctgga tgctgaatcg tacatcagcg gagcggccgc
      121 ccccaacgga gccggtcgag ccgggtgggg ataatgccct cgtgttcgag gatcgatcgg
      181 atgcgggccg gcggctggcg gaggcgcttc cggaggagga gtcatcgtcg gtggtgatcg
      241 ggatcccgcg aggaggggtc gccgtcggcg ccgaggtcgc ccggcttcga gggtgggacc
      301 tcgacgtggt ggtcccgcgg aaggttcgcg ccccgcagaa ccccgagctg gggatcggcg
      361 cgatcgctcc gggggtccgg ctcctcgacg agggcatgat cgccaccctg gcggtcgatc
      421 cggactacct ggtccgtgaa gtggccgacc aggagcgaga gatcgcgcgg cgcctcgcgg
      481 cgtaccgcgg cgaccggcca ccgctcgacc tcgccggccg gacggggatc gtcatcgacg
      541 acggggtcgc cacggggagc accgcggtcg ccgccgtgcg ttcggtccgt gcggccggcg
      601 cgaggagggt cgtggtcgcg gttcccgtcg cccccgcgga ggccgtcgag cggctgcgca
      661 gggaggcgga cggggtggtc tgcctcatca cgcccgctcg tttccacgcg gtcggggagt
      721 ggtatcggcg gttcccgcag gtgcaggatc ccgaggtcgt cgcccacctg gacgccgcat
      781 ggtcgtgatc gagatcgcgc tcgggggcct atgcctcgcg gtgggcgtct ggtcggccgt
      841 cggttcggtc cgcgccctct cggccgagga gccggggccg gaccggcggg tgctggtgct
      901 ccacgaatgg gcgaaggcgg ggttctggct cacgctcggc gcgttcctgc tcggctacct
      961 ggtgctggac gacccgcgcg agatccgagg gttcgccctc gtcccgatcg tcctggcggg
     1021 gatcaggttc ctcgcggcgg tccgcctggc gcggcgctga ggccgcatcg cccgcggctc
     1081 aaccacttcg ccctcgcggc cgatacctcc tccagacgat cgatcgcacc ggcgcccggg
     1141 gtgccggata tggagccatg acgtgaagcc gacggtgtcg ttggagtcga tgctgggagc
     1201 gcagcggggc gacccgccgg ctgcgctcat cccgcgctac ctcgccgaag cgaggaggca
     1261 gctggggatg gaggtcgcct acgtcgcgga gttccgcgac ggccagcgga tctaccggtt
     1321 catcgagggc gagacgatcc ccgacggcgt ccgtgtcggc accggcgatc cgctcgagga
     1381 caccttcgcc cagcgagtga tcgagggcag cgtcgacgcc gtgcctgacg tcgcacgcga
     1441 tccggccctc gcttcgctcg tcgagacgcg ttcctcgcgg atcgcgtcgt tcgtgggggt
     1501 gccgctgcgc ctcggcgtgg acgacatcta cggcatcctc gcctgccaga gctcgacgcc
     1561 gacgccgtcc ctcgacgatc gcgccgtgag cttcctccat gtgttcggcc ggctcatcct
     1621 cgaccagctc gacaaggagc agggcgagtg ggacgacctc cggcgccaga tcgaacgcgt
     1681 cgagcaggcc ctccagccgg gaagcatcgg tatcgagttc cagccggtct tcgacatccg
     1741 cagcgatgac ggccgcctcg tgggggccga ggcgctcgct cgtttcgagc tggagcccca
     1801 gcgcagccca gacgtctggt tcgccgaagc ctggacggtc ggacgcggcg tcgacctcga
     1861 gctcgcctcg gtcgaagcgg ccgtggccca cctgggccgc ctgccgcagg gaaccttcct
     1921 ggcgatcaac gtggcggcag agaccgtggt ctcaccgcga ctgcccggga tcctcgcggg
     1981 cgtgccgcgc gaccgggtcg tgctcgagat cccggag
//
LOCUS       DATLGG010000150         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_136725, whole genome shotgun sequence.
ACCESSION   DATLGG010000150 DATLGG010000000
VERSION     DATLGG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136725"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VM638_04270"
     CDS             complement(<1..854)
                     /locus_tag="VM638_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11678.1"
                     /translation="MTTPGGNEQPHVPPPSLWPVGFAVGIAILLTGLVVGPVIAVLGA
                     VLTVGFGFLWVRDLRTEATPLPQPPDDVETQPGAAPAAAGTGPALPLATEEEIERFPR
                     SKFLEGATLGLGAAIGGLVTVPALGFALAPAFAEGEQHEADVGPLDAFPQGEWRVTTF
                     LADPAQGEVTRRTAYIRYNGQVEGQPSFTILSSSCVHLGCPVQPNGPIAEESAEEEEI
                     GENVVRRIPTVPAGFGCPCHGGAYDLEGNRTAGPPVRAMDRWAYVVRNGRLMLRETFS
                     VGSVQGQGA"
     gene            complement(889..2388)
                     /locus_tag="VM638_04275"
     CDS             complement(889..2388)
                     /locus_tag="VM638_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HVM11679.1"
                     /translation="MAPPRDLREWIALLEREGELVRVSTEVDPDLEVTEIVDRTVKAG
                     GPALLFERPKGSRHPLLINQFGTERRMCLAFGVERLDDLGDRVADLLELQPPQGLVDK
                     VRGLKKLKSIADSGPRSVRSGPCQEVVLTGDDVDLDRLPIQRCWPGDPAPFITLPAVI
                     TRDPRTGTRNVGMYRMQKIDSKTTFMHWQIHKDGRADWLASEGRIDVAVALGLDPVTT
                     YSASAPLPKHMGELMLAGFFRGEAVEMVKCKTVDLEVPANAEIVLEGTIDHGDMGIEG
                     PFGDHTGYYTPPEPFPILRLSAITMRRDAIYPSIVVGKPPQEDAWLGKATERIFLPAV
                     RMTVPEIVDYDLPVSGTFHNCAIVSIRKRFPGHARKVMHAIWGLGMLSLTKAIVVVDE
                     DVNVHDYEEVFFYVGANVDPKRDIVITEGPLDHLDHAPTLQFFGGKIGIDATAKGPLE
                     GTREWPEEIAMAPEVKALVDERWREYGIDLGGGSRNGNAPAPLRRLLRR"
     gene            complement(2500..2799)
                     /locus_tag="VM638_04280"
     CDS             complement(2500..2799)
                     /locus_tag="VM638_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11680.1"
                     /translation="MDRHEEGREVYDMDGVAEARLEKIDGIHERLRGYVDPLAVQLDV
                     LRLLAHSASAGAGGAGAEAAREYDSARRHLDRFQRSLSRARSGRLRAHASRAPRR"
BASE COUNT          400 a         1052 c         1098 g          424 t
ORIGIN      
        1 gcgccctggc cctggacgga gccgacgctg aacgtctcgc ggagcatcag ccggccgttg
       61 cggacgacgt acgcccagcg gtccatcgcg cggacgggcg gcccggcggt gcggttgccc
      121 tcgaggtcgt acgcgccgcc gtggcagggg cagccgaagc cggcggggac ggtggggatc
      181 cggcgcacga cgttctcgcc gatctcctcc tcctcggcgc tctcctcggc gatcgggccg
      241 ttcggctgca cggggcagcc gaggtggacg caggacgagg agaggatcgt gaagctcggc
      301 tgcccctcga cctggccgtt gtagcggatg tacgccgtgc ggcgggtgac ctcgccctgg
      361 gcggggtcgg cgaggaacgt cgtcacgcgc cactcgccct gcgggaaggc gtcgagcggc
      421 ccgacgtcgg cctcgtgctg ctcgccttcg gcgaaggcgg gcgcgagcgc gaagccgagc
      481 gcgggaacgg tgacgagccc gccgatcgcg gcgccgagcc cgagggtcgc tccctcgagg
      541 aacttgctgc gcgggaaccg ctcgatctcc tcctcggtcg cgagcgggag cgcggggccc
      601 gttccggcgg cggcgggcgc cgcgcccggc tgcgtctcga cgtcgtccgg aggctgcggc
      661 agcggggtcg cctccgtgcg caggtcgcgc acccagagga agccgaaccc gaccgtcagg
      721 acggccccga ggacggcgat cacggggccg acgacgaggc ccgtgaggag gatcgcgatg
      781 ccgacggcga agcccaccgg ccacaggctc ggcggcggga cgtgcggctg ctcgttgccc
      841 cctggggtcg tcaccgtcgc tgattcaatc cttccacagc ggcagaggtc aacgtcgcaa
      901 cagccgccgc aagggagcgg gggcgttgcc gttccgagag ccgccgccga ggtcgattcc
      961 gtactcgcgc cagcgctcgt ccacgagcgc cttcacctcc ggcgccatcg cgatctcctc
     1021 cggccactcg cgggtcccct cgagcggccc cttcgccgtc gcgtcgatcc cgatcttgcc
     1081 gccgaagaac tggagcgtcg gggcgtggtc gaggtggtcg agcgggccct cggtgatcac
     1141 gatgtcccgc ttcgggtcga cgttggcgcc gacgtagaag aagacctcct cgtagtcgtg
     1201 cacgttcacg tcctcgtcga cgacgacgat cgccttcgtc aggctgagca tcccgaggcc
     1261 ccagatcgcg tgcatcacct tgcgcgcgtg gccggggaag cgcttgcgga tcgagacgat
     1321 cgcgcagttg tggaacgtcc ccgagaccgg caggtcgtag tcgacgatct ccggcacggt
     1381 catccgcacg gcggggagga agatccgctc ggtcgccttg ccgagccacg cgtcctcctg
     1441 cggcggcttg ccgacgacga tcgacgggta gatcgcgtcg cgccgcatcg tgatcgccga
     1501 gagccgcagg atcgggaacg gctccggcgg cgtgtagtac ccggtgtggt cgccgaacgg
     1561 cccctcgatc cccatgtccc cgtggtcgat cgtgccctcg aggacgatct ccgcgttggc
     1621 gggcacctcg aggtcgacgg tcttgcactt gaccatctcg acggcctcgc cgcggaagaa
     1681 gccggcgagc atcagctcgc ccatgtgctt cgggagcggc gccgaggcgg agtacgtcgt
     1741 gacggggtcg agcccgagcg cgacggcgac gtcgatcctc ccctcggagg cgagccagtc
     1801 cgcccgcccg tccttgtgga tctgccagtg catgaaggtc gtcttcgagt cgatcttctg
     1861 catccggtac atgccgacgt tgcgcgtccc cgtgcgcggg tcgcgcgtga tcactgccgg
     1921 gagcgtgatg aacggcgccg ggtcgccggg ccagcagcgc tggatcggga gccggtcgag
     1981 gtcgacgtcg tcgccagtga ggacgacctc ctggcacggc ccggagcgga cgctgcgcgg
     2041 gccggagtcg gcgatcgact tcagcttctt gaggccgcgg accttgtcga cgaggccctg
     2101 cggcggctgg agctccagca ggtccgcgac gcggtcgccg aggtcgtcga gccgctcgac
     2161 gccgaacgcg aggcacatgc gccgctcggt gccgaactgg ttgatcagca gcgggtgccg
     2221 cgagcccttc gggcgctcga acaggagggc ggggccgccg gccttcaccg tgcggtcgac
     2281 gatctccgtc acctcgaggt cggggtcgac ctcggtggag acgcgaacga gctccccctc
     2341 gcgctcgagc agcgcgatcc actcgcggag gtcgcggggc ggggccacag gatcgagtgt
     2401 acggactgcc ggacggccga cgccgcgccc ggaggccgga gccgtcgcac gcggtgctcc
     2461 cgacgatgcc accgcgtgcg acgcccggcc ggcgcgcgcc taccgccgag gcgcgcgact
     2521 cgcgtgcgcc cgcagccgcc cgctgcgggc gcgggagagg ctgcgctgga agcggtcgag
     2581 atgccggcgc gcggagtcgt actcgcgtgc ggcctcggcg cccgcgccgc cggcgccggc
     2641 cgaagcgctg tgcgcgagca gccgcagcac gtcgagctgc accgcaagcg ggtccacgta
     2701 acccctcagc cgctcgtgga tgccgtcgat cttctccagc cgcgcctccg cgacaccgtc
     2761 catgtcgtac acctctcgcc cctcctcgtg tcgatccatg tccagtgttc gtccaagctg
     2821 gtggatcggt tcgacgcacg cggacgagcg gcgatgcgga ggcgagagtc tgcgcgctgc
     2881 ggctagcgcg ggccgcggtc gtgccggcgc cagtgcacgt actcgcccac gatcacgatg
     2941 ctcgcgaaga tgaggccgac gatcagcatc gcgg
//
LOCUS       DATLGG010000151         7203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_88741, whole genome shotgun sequence.
ACCESSION   DATLGG010000151 DATLGG010000000
VERSION     DATLGG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88741"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..957
                     /gene="lipA"
                     /locus_tag="VM638_04285"
                     /pseudo
     CDS             35..957
                     /gene="lipA"
                     /locus_tag="VM638_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796527.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(924..1454)
                     /locus_tag="VM638_04290"
     CDS             complement(924..1454)
                     /locus_tag="VM638_04290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVM11681.1"
                     /translation="MLKRALPVIGVALAGTIVLTLVALGGGRGATAPSVETSGPMPAL
                     DEPAMRGEGRIRTEELRGKIVLVNFWASWCGPCRLEQPALERLSQEFDEEVAFLGVNF
                     GDDRAAALEYLREFDVSYPSVEDRDGRIAHRFLVPYLPATVLVDRTGEMRYRLLGEQT
                     EESFRRYLVALLREAP"
     gene            complement(1469..1814)
                     /gene="rnpB"
                     /locus_tag="VM638_04295"
     ncRNA           complement(1469..1814)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VM638_04295"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1895..2290)
                     /locus_tag="VM638_04300"
                     /pseudo
     CDS             complement(1895..2290)
                     /locus_tag="VM638_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
     gene            complement(2254..2964)
                     /locus_tag="VM638_04305"
     CDS             complement(2254..2964)
                     /locus_tag="VM638_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HVM11682.1"
                     /translation="MKGTERLLELQALDLAIDRLRARRAVLESGEEVRAAREKADALE
                     DEIGEIRMALDGVRREQERLESEVRSFEAKIAAEEKRLYDGSVANPKELGSIQHEVAN
                     LRQRTARVEDEVIVQMERREELEERLPSLEADLASARRGQEELEASSATELEQVTEDL
                     ARREEERSALTPVFDEELLELYEDLRAQKRGVGAAALRDGVCGGCHQKLSPLELEKVR
                     KAEGVRRCDYCRRILVLD"
     gene            complement(2961..4064)
                     /locus_tag="VM638_04310"
     CDS             complement(2961..4064)
                     /locus_tag="VM638_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HVM11683.1"
                     /translation="MAPAPSVSDRLAVIDGLFPPSWAEPWDNVGLQLGDRHAAAERVL
                     VALDPTIDVIREAAGRGCGLLVTHHPLVFEPLRSLDLAAPLAGAVAEAIRSGVAVAAC
                     HTNADVARPGVSDALAEALDLRVEGVLRPTTAGRRVKVTTFVPPEATEKVLDAMAAAG
                     GGRIGEYSACSFRVRGTGTFVPSAAADPAVGERGTLNQVEEDRLEMVVPGHLVGPLVA
                     AMIEAHPYEEVAYDVVPLEGGEELGLGRITRTEDAISAGDLLERCRERLGPGARIVGD
                     VSRPLTTIAVCGGSGTSLIPDALAADADAFVTGDVKHHQALDAIAAGLVLIDAGHHAT
                     EWPFVLRLAARLRGAMPDAEILLSETRTEPFAP"
     gene            4131..4304
                     /locus_tag="VM638_04315"
     CDS             4131..4304
                     /locus_tag="VM638_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11684.1"
                     /translation="MRRRLIIALSALCLALPACGQNSPGGPTGPAEPGAPQQPPGDQP
                     VGPPGYPADDPAA"
     gene            complement(4301..4738)
                     /locus_tag="VM638_04320"
     CDS             complement(4301..4738)
                     /locus_tag="VM638_04320"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HVM11685.1"
                     /translation="MSTLTLVAGLVMMAAITVYLVYVLVRTIRSEARKRNIRKIWANF
                     GLSLVLMILFFGSWIGHAFAQWSDYVAEQEQHQEPAVVSEYLAEFSKATLENWQSEFL
                     QLFSFVVLAAIFLHRGSAESRDSDDEIQASLQRIEAALNTKES"
     gene            4868..6082
                     /locus_tag="VM638_04325"
     CDS             4868..6082
                     /locus_tag="VM638_04325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVM11686.1"
                     /translation="MHRPRVVLLTAHLPFPPISGGRRREYELIRRLGDRADIDLCAIS
                     KTPQEDRANAPRLEHWCRSVAVFPALSPDAPGRRTDPWVVRRHRAPGAAEHIRTRLDE
                     ADLVHLEGFYLSQHLPLDLPVAALLVEHNVEYLLWRQRAAATARRAERARHLLEYART
                     LEAEIAAWKRASIVGALTPEDREAMLQADHGLDVRLLPNGSDLRAPEPSGEDPIVLPK
                     TPRIAFVANFAYQPNVDAALHLARDILPRIRKAVPEVALMLVGNGPPDEVRALAGDGI
                     VVTGRVPSVAPYLAAADVVVCPLRVGGGAKVKISEAIAEGKAIVTTSVGAQGLPEEAS
                     RCMRIEDRPAPLARAVVELLRDPPERRRLERAASELAASLPTWDEAADRVLACYQELT
                     TRRLRSVRGAAS"
     gene            6426..7103
                     /locus_tag="VM638_04330"
     CDS             6426..7103
                     /locus_tag="VM638_04330"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVM11687.1"
                     /translation="MSYTKVVRCGELDCIVPLDVLAPPDEADLPTIVLIGGDGTFDQR
                     RYLDVLAGEVARRGAVVFLSSYRSATTVNGLTDSLHDVRCSVRYARSVTAEHGGDPQT
                     VVLVGHSYGSNLALQTAVTLDAETDGCLATGDGVPEAVVGLSEFHVASMADVPSPPPP
                     VLLGGGSDDPLSETGPKTEEQLREAGFEAEYREFSDTGHEDLIDPRLTPGVLDLIFEA
                     VASAEAG"
     gene            complement(7134..7197)
                     /locus_tag="VM638_04335"
     tRNA            complement(7134..7197)
                     /locus_tag="VM638_04335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7171..7173),aa:Val,seq:tac)
BASE COUNT         1035 a         2683 c         2429 g         1056 t
ORIGIN      
        1 ccagaccgag accccctcgg cgccgcaggc ggacctgacc gacacgcacg cggcgcactc
       61 gatcgggtgc gcccagaccg ccacgcccgt tcggcgtccg ccgtggctga agacgaaggc
      121 tcggttcggc cccaactacc gcgacctcaa ggggatcatg aacggcctcc agctcaacac
      181 cgtttgcgag gaggccggct gcccgaacat ctacgagtgc tgggaggacc gcgaggcgac
      241 cttcctgatc ctcggcaacg tctgcacccg ccgctgcggg ttctgcgacg tgcagacggc
      301 gccgacgagc ccgcccgcat cgccgaggca gtcaaggcga tgggcctcaa cttcgtcgtc
      361 ctcaccggcg tcgcgcgcga cgacctcccc gacggtggcg cgagcgtctg ggcggactgc
      421 atccgggcga tccgcaacgc ggtgcccggc tgcgggatcg aggtcctgcc gccggacttc
      481 aaggcgcacc gcgagcagat cgcgaccgtc atcgaggccg agcccgacgt gttcgcgcac
      541 aacctcgaga ccgtccgccg cctacacaag cggatccgcc ccgggttcga ctacgaccgc
      601 accctcgaag cgctgcggct cgcgaaggcg ctccggcccg gccaggtcac gaagtccaac
      661 ctgatcctcg ggatgggcga gcgccccgag gaagtgacgc aggcgctccg cgacctgcgt
      721 gaggcggacg tggatctgat gacgatcggc cagtacctcc agccgacgaa gttccacctc
      781 cccgtcgacc gctgggtcac ccccgaggag ttcgaggaac acgcgcgggc cggcaaggag
      841 atgggcttcg cctgggtcga gtccggtccg ctcgttcgat cgagctacca cgccggtgag
      901 cagctcgcgc gagcccgcgc ggctcagggc gcctcgcgca gcaacgccac caggtagcgc
      961 cggaaactct cctcggtctg ctcgcccaac aggcggtacc gcatctcccc cgtccggtcg
     1021 acgaggacgg tcgcgggcag gtacgggacc aagaagcggt gcgcgatccg gccatcgcgg
     1081 tcctcgacgc tcgggtagct gacgtcgaac tcgcggaggt actcgagagc agcggcgcgg
     1141 tcgtccccga agttcacgcc gaggaaggcg acctcctcgt cgaactcctg cgagaggcgc
     1201 tcgagcgcgg gctgctcgag gcggcagggc ccgcaccacg aggcccagaa gttcacgagc
     1261 acgattttcc cgcgaagctc ttcggtgcgg atgcggccct ccccgcgcat cgccggctcg
     1321 tcgagggcag gcatcgggcc gctcgtctcg acggagggag cggtcgcccc acggccgccg
     1381 ccgagggcga ccagcgtgag gacgatcgtc cccgcgaggg cgacgccgat gaccgggagg
     1441 gctctcttca gcatctcgtt gaacggtagc aaggagccgg cctgtaagcc ggattctgtc
     1501 ccccgaaggg gggcggccat ccatctggga gccgcgttgc cgcggccctc atgcggccta
     1561 cccgggagct cgggcgggcc gccctcgaac gctccctgct tggccttgct ccgggtgtgg
     1621 tttgccgagc cgcgacggtc acccgccgcg ctggtgagct cttacctcac cgtttcaccc
     1681 ttacccccga gggggcggtc tgctctctgt ggcactttcc gcgggtcacc ccgcctgggc
     1741 gttacccagc accctgccct gtggagtccg gactttcctc ggccctttcg gaccgcggcc
     1801 gcccggccgg ctcctccgga ccagtatagg ggccgccacc gcgggcagga cgccgtcgtg
     1861 cggcgagcca cgcatcgacg ccgtcgttcg ccagccggtc ggcatgggcg ttcagcgcgc
     1921 gcgggacgtg cgcgagcgtc accttctcga acgagcggac cagctcgagc gcctccgcgt
     1981 ggaggggccg gaggtgctcg gtgcggacct tgaacctgcc cgccagctgc tcgatcaaca
     2041 gccggctatc ggagcggagc atgacgcggg atgcaccgtg gcgggcggcc agacgcagcc
     2101 cctcgatcgc cgcgcggtac tcggcgacgt tgttcgtcgt ctcaccgatc ccctccgcca
     2161 gcacggcgat gacgttgcca tccccgtcgg tcacgaccac cccgatcccc gccggcccgg
     2221 ggttgccgcg ggcggccccg tcggtggcga cgatcagtcg aggaccagga tccgccggca
     2281 gtagtcgcac ctccggacgc cctcggcctt acggaccttc tcgagctcca gcggactcag
     2341 cttctggtgg cagccaccgc agacgccgtc ccgcagcgcc gcggccccca cccctcgctt
     2401 ctgcgcccgg agatcctcgt agagctcgag cagctcctcg tcgaacaccg gcgtcagcgc
     2461 cgagcgctcc tcctcccgcc gagcgagatc ctccgtgacc tgctcgagct cggtcgcgga
     2521 cgaagcctcc agctcctcct gaccgcgtct ggcggaggcc agatccgcct ccaggctcgg
     2581 gaggcgctcc tccagctcct cgcgtcgctc catctgcacg atgacttcgt cctcgacccg
     2641 cgccgtgcgc tgccgcaggt tcgcgacctc gtgctggatc gacccgagct ccttgggatt
     2701 ggcgaccgac ccgtcgtaga ggcgcttctc ctccgccgcg atcttcgcct cgaacgagcg
     2761 gacctccgac tcgagccgct cctgctcgcg ccggacgccg tcgagcgcca tgcggatctc
     2821 cccgatctca tcttcgagcg cgtccgcctt ctcgcgggcg gcgcgcacct cctcccccga
     2881 ctcgaggacg gcgcgccgtg cccgcagccg gtcgatcgcc aggtcgagcg cctggagctc
     2941 gaggagacgt tccgtgccct tcacggggcg aacggctccg tgcgcgtctc ggacaggagg
     3001 atctccgcgt ccggcatcgc cccgcgaaga cgcgcggcga ggcgtagcac gaacggccac
     3061 tcggtcgcgt ggtgcccggc gtcgatgagg acgaggcccg ccgcgatcgc gtccagcgcc
     3121 tggtgatgct tcacgtcgcc ggtgacgaac gcatcggcgt ccgcggcaag tgcgtcgggg
     3181 atcagcgagg tccccgaccc gccgcagacg gcgatcgtcg tgagcgggcg cgagacgtcg
     3241 cccacgatcc gcgcgccagg gccgagccgt tcgcggcagc gctcgaggag gtcgccggcg
     3301 ctgatcgcgt cctccgtgcg cgtgatccgg ccgagcccga gctcctcccc gccctcgagg
     3361 gggacgacgt cgtaggcgac ctcctcgtac ggatgggcct cgatcatcgc cgccacgagc
     3421 ggtcccacca ggtgtccggg gaccaccatc tcgagccgat cttcctcgac ctggttcagc
     3481 gtcccgcgct ccccgacggc cgggtccgcc gccgccgaag gaacgaacgt cccggtcccg
     3541 cggacacgga acgagcaggc ggagtactcg ccgatccttc caccgcccgc ggccgccatc
     3601 gcatcgagga ccttctccgt ggcctcgggt ggcacgaacg tcgtcacctt cacccggcga
     3661 cccgccgtgg tcggacgaag caccccctcg acccggaggt cgagcgcctc cgcgagagcg
     3721 tcgctgacac cggggcgggc gacgtcggcg ttcgtgtggc aggcggcgac ggcgacgccg
     3781 gagcggatgg cctccgcgac ggccccggca aggggggccg cgaggtccag gctccggagc
     3841 ggctcgaaga cgagcggatg gtgcgtcacg aggagaccgc agccccgccc ggcggcctcg
     3901 cggatgacgt cgatcgtcgg gtcgagcgcg accaggaccc gctccgcggc cgcgtgccgg
     3961 tcgccgagct gaaggccgac gttatcccac ggctccgccc agctcggagg gaagagcccg
     4021 tcgatgacgg cgaggcggtc ggagacggag ggagccggag ccatgggttc cgtagcatat
     4081 cgcccggcga ccgcccgttt gcctccacgc cgcctggata ggatgagcgc atgcgccgcc
     4141 gcctcatcat cgctctcagc gccctctgcc tcgcccttcc ggcctgcggc cagaactccc
     4201 cgggcgggcc cacgggtcct gcggaacccg gagcgccgca gcagcccccc ggcgaccagc
     4261 cggtcgggcc gcctggctat ccggccgacg acccggccgc ctagctctcc ttcgtgttca
     4321 gcgccgcctc gatgcgctga agcgaggcct ggatctcgtc atcgctgtcg cgtgactccg
     4381 cgctcccccg gtgcaggaag atcgccgcga gcacgacgaa cgagaacagc tggaggaact
     4441 ccgactgcca gttctcgagg gtggccttgc tgaactccgc caggtactcg ctcacgaccg
     4501 ccggctcctg atgctgctcc tgctcggcga cgtagtcgct ccactgcgcg aaggcgtggc
     4561 cgatccagct cccgaagaac aggatcatga gcacgaggct cagcccgaag ttcgcccaga
     4621 tcttgcggat gttgcgcttg cgcgcctcgc tcctgatcgt ccggaccagg acgtacacca
     4681 ggtagaccgt gatggcggcc atcatcacga ggcccgccac gagcgtcaac gtcgacatcg
     4741 ctcccctctc aatctcgggt gccgggaggg gcattccccg gggcggtgga atcaaaccga
     4801 ccaacggctc ggtttgccgc tcaggaggcg ggggtatccg cagactgccg gaggggaagg
     4861 agatcgcatg caccgtccgc gggtcgtgct cctgaccgcg cacctgccgt tcccgccgat
     4921 cagcgggggg cgccgccgcg agtacgagct gatccgacgt ctgggcgacc gggctgacat
     4981 cgacctctgc gcgatctcga agacgccgca ggaagatcgg gcgaacgccc cgaggctcga
     5041 gcactggtgc cgctcggtcg ccgtcttccc ggcgctctcc cctgacgctc ccggtcgtcg
     5101 cacggacccg tgggtggtgc ggcggcaccg ggcgcccggg gccgccgagc acattcgcac
     5161 gcggctggat gaggcggacc tcgtccacct cgaggggttc tacctcagtc agcacctgcc
     5221 gctggacctt cccgtcgcgg cactcctcgt cgagcacaac gtcgagtacc tcctgtggcg
     5281 gcagcgtgcc gccgccaccg ctcgccgcgc cgaacgcgcc cgtcacctgc tggagtacgc
     5341 gcgcacgctc gaggcggaga tcgccgcctg gaaacgggca tcgatcgtcg gtgcgctcac
     5401 ccccgaggat cgcgaggcga tgcttcaggc ggatcacggc ctggacgtgc gcctgctccc
     5461 gaacggatcg gatctgcggg cgcccgaacc gtccggcgag gatccgatcg tcctgccgaa
     5521 gacgccgcgg atcgccttcg tcgcgaactt cgcctaccag ccgaacgtgg acgcggccct
     5581 ccacctcgcg cgagacatcc tgccgcgtat ccgcaaggcc gttccggagg ttgccctgat
     5641 gctggtcgga aacgggccgc cggacgaggt ccgtgcgctc gcgggggatg gcatcgtcgt
     5701 caccgggcgg gtcccctcgg tcgccccgta cctcgccgcc gccgacgtcg tcgtctgtcc
     5761 gctgcgggtc ggcggaggcg cgaaggtgaa gatctccgag gcgatcgcag aagggaaggc
     5821 catcgtgacg accagcgtgg gagcgcaggg cctgccggag gaggcctctc gctgcatgcg
     5881 gatcgaggac cggccggcgc cgcttgcccg cgccgtcgtc gagctcctgc gagatccgcc
     5941 ggagcgtcgg cgcctcgagc gagccgcatc cgagctggcg gcgtcactgc cgacgtggga
     6001 cgaggcggcg gaccgcgtgc tcgcctgcta tcaggagctc acgacccgcc gcctccgcag
     6061 cgtccgcggc gccgcgagct gaagcccgcg gcgcgagcaa ccccgcagct gcgctctcca
     6121 cagcccgggt tgggaatcgg ctcccgccga ggtgaaaagc gactcgtctc tccatggcga
     6181 cactcatgta gagtgcgtcc gcgaccgcta cctcgttcgg agtgaaagcc ttgccagctt
     6241 ccacgaagtt gcttaccgcg ctcatcgcga gccttgcgcc cctccttgtc tcgtgcgggg
     6301 acgaccagag gccctccgcc tctctcgcgc agcccgatcc ggcctcacca cgaccgacgc
     6361 tcgccgcgcc accagcgggc gacttcagcg ggccggtcgc ggagggtctt cgcgccgagg
     6421 tcgacgtgag ctacaccaag gtcgtccgat gcggcgagct cgactgcatc gtcccgctcg
     6481 acgtgctggc acctcccgat gaagcggacc tcccgacgat cgtcctcatc ggtggggacg
     6541 gcacgttcga tcagcgccgc tacctcgacg tactcgcagg cgaggttgcg cgtcggggtg
     6601 cggtcgtctt cctgtcctcc taccgaagcg cgaccacggt caacggcttg accgactccc
     6661 ttcacgacgt tcgctgctcc gtccgctacg cccgctcggt cacggctgag cacggcgggg
     6721 atccgcagac agtggtgctg gtgggccact cgtacggcag caacctcgcg ctgcagacgg
     6781 ctgtgacgct cgacgcggag accgatgggt gtctcgccac cggcgacggg gtccccgaag
     6841 cggtcgtcgg cctcagcgaa ttccacgtcg cgtcgatggc cgatgttccc tctccgccgc
     6901 ccccggtgct gcttggcggt ggctccgacg atcccttgtc cgagaccggg ccgaagaccg
     6961 aagagcagct gcgcgaggcc ggcttcgagg cggagtaccg cgagttctcg gataccggcc
     7021 atgaggacct catcgacccg aggctcactc ccggcgttct cgacctcatc ttcgaagccg
     7081 tggcatccgc cgaagcgggc tgagtccgcg ccggtccggc tccagggtgt gcgtgggcgc
     7141 tgctggtttc gaaccagcga cctccgcctt gtaagggcga accagggggg ttcgccctta
     7201 caa
//
LOCUS       DATLGG010000152         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_166971, whole genome shotgun sequence.
ACCESSION   DATLGG010000152 DATLGG010000000
VERSION     DATLGG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166971"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VM638_04340"
     CDS             <1..1243
                     /locus_tag="VM638_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11688.1"
                     /translation="AGHNVDASATCGGGRFVSTGGAGTAFEFDVKSPDEGFWNSGVTV
                     TLTGPNAASVGANSANAMQLQYNDEGQWQTIAYHFRQGCSPVGCSNNPGGAGADVYVP
                     AGQVVTANEPKPGTYRVFFSNSASLPGRIKVDFHTAPAEPVPLQEKIDASSMDFFDDL
                     NRYIPDGSDTEPIQVADVIARPERLASFDSIVVVNKLGSRAYVTSDLGLSAAEANAYF
                     AGLKRFAQEGGNLVLTDAALQALPELGIVPASTVAMLTDATTQSGNYAFEIASGNVTY
                     RNPSRYPLTAGVNKPGAAEQELGRRQAVEPVPLGYTPETGLDGTPRIPFWRVERTAWQ
                     NACGQPDCAAAVTHSRGTNVNLGEARLGAGRVRIAGIMFPDPVFEPQPLVNDHRFGLA
                     SYALTYTGWQVFENLIDYRRP"
     gene            1392..>2301
                     /locus_tag="VM638_04345"
     CDS             1392..>2301
                     /locus_tag="VM638_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11689.1"
                     /translation="MKIRSAILTAAVALAGLPLAGSGSSALAVPNPVATDEATYQAFG
                     RVFPDPHGCLAHNVPDQDGNGVKDTPKGVSPWAKGRVCADQFLQYEEVIEGAKFLARR
                     LPEFVSVIRLDQAYDNPNFMSAGIARNVVTEDGKVKTLGRDRRPLYMFKVTDRTSPIA
                     EKDREHFVYSLSIHGIERAGVEGGVRAMEDLATWAACEREKYASPEAPALPLPACGIE
                     GPYPKRIVETPTNAAVPTAGDALRGSVVYFVLANPDGWARGQVAPVEVEDGSPNTNYT
                     PGFFFQRYNGNGVDLNRDWPTEGYSFK"
BASE COUNT          371 a          807 c          802 g          321 t
ORIGIN      
        1 ggcggggcac aacgtggacg cctcggcgac ctgcggcggc ggtcgcttcg tctcgaccgg
       61 cggggcgggg acggcgttcg agttcgacgt gaagtcgccg gacgaggggt tctggaactc
      121 cggcgtgacc gtcacgctca ccggaccgaa cgcagcgagc gtcggggcca actccgccaa
      181 cgcgatgcag ctccagtaca acgacgaggg acagtggcag acgatcgcct accacttccg
      241 gcagggttgc tcgccggtcg ggtgctccaa caacccgggc ggggcgggcg ctgacgtcta
      301 cgtgccggcc ggtcaggtcg tgaccgcgaa cgagccgaag cccggcacct accgcgtctt
      361 cttctcgaac tcggcctcgc tgccggggcg gatcaaggtg gacttccaca ccgcccccgc
      421 agagccggtg ccgctgcagg agaagatcga cgcctcgtcg atggacttct tcgacgacct
      481 caaccgctac atccccgacg ggtccgacac cgagccgatc caggtggcgg acgtgatcgc
      541 caggccggag cgcctcgcga gcttcgactc gatcgtggtg gtcaacaagc tcgggtcgcg
      601 ggcctacgtc acctccgacc tgggcctctc cgccgccgag gcgaacgcgt acttcgccgg
      661 actgaagcgg ttcgctcagg aaggcgggaa cctcgtcctg acggacgcgg ctctccaggc
      721 tctgcccgag ctggggatcg ttcccgcctc gacggtcgcg atgctcaccg acgcgacgac
      781 gcagtccggg aactacgcct tcgagatcgc gagcggcaac gtgacctacc gcaacccgtc
      841 gcggtacccg ctcaccgcag gcgtcaacaa gccgggtgcg gcggagcagg aactcggtcg
      901 tcggcaggcg gtcgagcccg tgccgctcgg ctacacgccc gaaaccgggc tcgacggcac
      961 gccgcggatc ccgttctggc gggtcgagcg gacggcgtgg cagaacgcct gcggccagcc
     1021 ggactgcgcg gcggccgtga cgcactcgcg cgggacgaac gtgaacctcg gcgaggcgcg
     1081 gctcggcgcc ggacgtgtgc ggatcgccgg catcatgttc cccgacccgg tcttcgagcc
     1141 gcagccgctc gtgaacgacc accggttcgg tctggcgtcc tacgccctga cctacaccgg
     1201 ctggcaggtg ttcgagaacc tgatcgacta ccgccgtccg tagcggccga gatcgcaggc
     1261 gaagggccgg ccttcgggcc ggcctttcgc gcgtgccagg ggcggtggaa ccgccaccgc
     1321 gagacgtagg cgccggggag agcggggcga acaccttcgg tgaggaaccc atcccacgaa
     1381 gggagctccc gatgaagatc cgctctgcga tcctgacggc ggcggtggcg ctcgccggat
     1441 tgcccctcgc cggctccggc agctcggcgc tggcggtccc gaaccccgtg gccaccgacg
     1501 aagcgacgta ccaggcgttc ggccgggtct tcccggaccc gcacgggtgc ctcgcccaca
     1561 acgttccgga ccaggacggg aacggtgtca aggacacccc caagggcgtc tcgccctggg
     1621 cgaagggtcg cgtctgcgcg gaccagttcc tccagtacga ggaggtgatc gagggagcga
     1681 agttcctcgc gcgccgcctt ccggagttcg tcagcgtgat ccgtctcgac caggcctacg
     1741 acaacccgaa cttcatgtcg gccggcatcg cgcgcaacgt cgtgaccgag gacggcaagg
     1801 tcaagacgct cgggcgggac cgccgtcccc tctacatgtt caaggtgacc gaccgcacct
     1861 ccccgatcgc cgagaaggac cgggagcact tcgtctactc gttgtcgatc cacgggatcg
     1921 agcgtgcggg cgtcgagggc ggcgttcgcg cgatggagga tctggcgacc tgggcggcgt
     1981 gcgagcgtga gaagtacgcg agccccgagg cgccggcgct cccgctcccg gcgtgtggga
     2041 tcgaaggccc gtacccgaag aggatcgtcg agacccccac gaacgccgcc gtgccgacgg
     2101 cgggtgacgc gctgaggggc tcggtcgtct acttcgtcct cgcgaacccc gacggctggg
     2161 cgcgcggtca ggtcgccccg gtcgaggtcg aggacgggtc accgaacacg aactacacgc
     2221 ccggcttctt cttccagcgc tacaacggca acggcgtgga cctgaaccgg gactggccca
     2281 ccgagggcta cagcttcaag c
//
LOCUS       DATLGG010000153         3517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_226937, whole genome shotgun sequence.
ACCESSION   DATLGG010000153 DATLGG010000000
VERSION     DATLGG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226937"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VM638_04350"
     CDS             complement(<1..272)
                     /locus_tag="VM638_04350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HVM11690.1"
                     /translation="MTGAVIVGLLAAGGVYLLLQRGIVRVTLGFVLLGHAANVLLVVS
                     GGVGRRGVPIAGEPGLSADPLPQAFALTAIVIGFAMTAFLLALAFR"
     gene            complement(269..685)
                     /locus_tag="VM638_04355"
     CDS             complement(269..685)
                     /locus_tag="VM638_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HVM11691.1"
                     /translation="MSTLILRTAARLLVPLIGVLAVYLLVRGHDAPGGGFIAALAGGA
                     AIVLRYLAHGPAGVRRLVPIPFSSLLGVGLLLAVGYGVAGLVAGAEFLRGAIWRVEAP
                     VVGELKVAAALVFDLGVFFVVLGVVLAIVRYLGEER"
     gene            complement(682..2967)
                     /gene="mbhE"
                     /locus_tag="VM638_04360"
     CDS             complement(682..2967)
                     /gene="mbhE"
                     /locus_tag="VM638_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HVM11692.1"
                     /translation="MATLPLILPAAMLVVAAVAPLAARWWGRSAGYAIAVAYAAGTGA
                     LLTAAPRVADGAELAAAVPWVPGLGIRLAFRLDGLSLLFGLLVLGVGALVAAYSARYL
                     KREGRHGRFYGLFTLFAASMLGLVTADDLFLLFVFWEMTSITSFFLIGGDGGPEASRS
                     ATRAFLLTSLGGLALFAALVLAGIAAGSFDLHGILERPGAIRDHAFAPAIMLLLFAAA
                     FTKSAQVPFHFWLPGAMVAPTPVSTYLHAATMVKAGIYVLARFSAVFAGDPTWTTTLV
                     LVGLTTAVVGAVLALKQHDLKALLAYSTVSQLGLLTALVGVGTFAAMAAACLHLLAHG
                     LYKASLFMVVGIIEHEAGSRDLRRLSGLRRLMPRTAAVTGIAALSMAGIPPLLGFVSK
                     EESLAGFLGGLPDGLALVAVTLAVLASVMTFAYGARIFDGAFAGTATQRLYDPPAGFL
                     APAALPAALGLLLGLVVWRLDPLVGPAAAAALGMEGETHLALWHGLTPGLALSGVAIG
                     AGVLLFAVRRPVEHVLARLRAPAAGERAFDRSHRAVVGTGKIAGRPFLAATPVRHLPW
                     VLATAAVAGVATWRLLGGSADGPPPPFPPPDRWLAGALALSVLALAWARNRLAAVAVL
                     GTVGFLVSVLWVLLGAPDLAITQLLVETLTVVLVVLVFRGLPKGFARSSGRRMAAGAG
                     AAVVVGGLAAWGTFLLTGRRQLSEPGAYFLEAAASEAGGKNVVNTILVDFRALDTLGE
                     ITVLAVAALGVFLLVRRTGER"
     gene            complement(3008..>3517)
                     /locus_tag="VM638_04365"
     CDS             complement(3008..>3517)
                     /locus_tag="VM638_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11693.1"
                     /translation="GLRGAHGFLLFLVAFALRRADNPAGWIGVLMIAGVAGVYLGDIL
                     APRLPRGIREELIVLVALAGAGGAALLAFTTFSLPTMALFTGMAGLATRFGQLAFASL
                     MQRHAPGEAHGRVFVRYEISFQLAWVGGAFAAALLPLDFRPGVALLGLFYLVVAAVYL
                     ARPLLAERG"
BASE COUNT          607 a         1308 c         1291 g          311 t
ORIGIN      
        1 ctgcggaagg cgagcgccag caggaacgcc gtcatcgcga acccgatcac gatcgcggtg
       61 agcgcgaacg cctgcggaag gggatcggca gagaggcccg gttccccggc gatcggcacg
      121 ccacgccgcc cgaccccgcc cgagacgacc agcaggacgt tcgcggcatg cccgagcagg
      181 acgaagccga gcgtgacgcg cacgatcccg cgctgcagga gcaggtagac gcctcccgcc
      241 gccaggagcc cgacgatcac cgcccccgtc atcgctcctc ccccaggtac cgaacgatcg
      301 cgagcacgac gccgagcacc acgaagaaga cgccgaggtc gaagacgagc gccgccgcca
      361 ccttgagctc cccgacgacc ggcgcctcga cgcgccagat cgccccccgg aggaactcgg
      421 ctcccgcgac gagccccgcg acgccgtagc cgacggcgag caggagcccg accccgagca
      481 gggaggagaa ggggatcggc acgagccgcc gaacccccgc cgggccgtgc gcgaggtagc
      541 gcaggacgat ggccgctccg cccgcgagcg ccgcgatgaa gcccccgccc ggcgcgtcgt
      601 gaccgcggac gaggaggtag acggcgagga cgccgatcag ggggacgagg agccgggcgg
      661 cggtccgcag gatcagggtg ctcatcgctc acccgtccgt cggacgagca ggaacacccc
      721 gagcgccgcc accgcgagca ccgtgatctc ccccagggtg tcgagcgccc ggaagtcgac
      781 gaggatcgtg ttgacgacgt tcttcccgcc ggcctcggat gcggccgcct ccaggaagta
      841 cgcgcccggc tccgacagct gccgccggcc cgtcagcagg aaggtgcccc acgcggcgag
      901 gccgccgacg acgaccgccg ccccggcgcc ggccgccatc cggcggccgc tggacctcgc
      961 gaaccccttc gggagtccgc ggaagacgag cacgacgagg accacggtga gggtctcgac
     1021 gaggagctgc gtgatcgcca ggtccggcgc tccgagcagg acccacagca ccgagacgag
     1081 gaagcccacg gtcccgagca cagccacggc cgcgaggcgg ttccgtgccc acgcgagcgc
     1141 gagcaccgag agcgcgaggg cgcccgccag ccatcggtcc gggggcggga agggaggcgg
     1201 tggcccgtcc gccgacccgc cgaggagcct ccacgtcgcg acgcccgcca ccgcggcggt
     1261 cgcgagcacc caggggaggt ggcgcaccgg ggtcgccgcc aggaacgggc gccccgcgat
     1321 cttgcccgtc cccacgaccg cgcggtggga tcgatcgaag gcgcgctcgc cggcggccgg
     1381 agcccgcagg cgggccagga cgtgctccac cggccggcgg acggcgaaca gcaggacgcc
     1441 cgcgccgatc gccacgcccg agagcgcaag cccgggcgtg aggccgtgcc acaacgcgag
     1501 gtgcgtctcg ccctccatcc cgagggccgc cgcagcggcc ggtcccacga gggggtccag
     1561 ccgccagacg acgagcccga gcagcagccc gagggccgcc ggcagcgccg cgggtgcgag
     1621 gaacccggcg ggtgggtcgt agagccgctg ggtcgcggtc cccgcgaagg cgccgtcgaa
     1681 gatccgcgcg ccgtacgcga acgtcatgac agaggcgagg acggcgaggg tgaccgcgac
     1741 gagtgcgagg ccatccggaa ggccgccgag gaacccggcg aggctctcct ccttgctcac
     1801 gaaccccagc aggggcggga tgccggccat cgagagcgcc gcgatcccgg tgaccgccgc
     1861 ggttcgcggc atcagtcgcc gcagccccga gagccgacgc aggtcccggc tcccggcctc
     1921 gtgctcgatg atgccgacca ccatgaacag ggacgccttg tagagcccgt gcgcgagcag
     1981 gtgcaggcac gcggccgcca tcgcggcgaa cgtgccgacg ccgacgaggg ccgtcagcag
     2041 gccgagctgg ctgacggtcg agtacgcgag gagcgccttg aggtcgtgct gcttcagcgc
     2101 gaggaccgcc ccgaccaccg cggtcgtcag gccgacgagc accagcgtcg tcgtccacgt
     2161 cgggtcaccg gcgaagaccg cggagaaccg cgcgaggacg tagatcccgg ccttgaccat
     2221 cgtcgccgcg tgcaggtagg tgctgaccgg cgtcggtgcc accatcgccc ccgggagcca
     2281 gaagtggaac ggcacctgcg ccgacttcgt gaaggccgca gcgaacagca gcagcatgat
     2341 cgcgggggcg aaggcgtgat cgcggatggc ccccgggcgc tcgagtatcc cgtgcaggtc
     2401 gaagcttccg gcggcgatcc cggcgaggac cagcgcggcg aacagcgcaa gaccgccgag
     2461 ggaggtgagg aggaacgcgc gcgtcgccga ccggctcgcc tcgggcccgc cgtcaccacc
     2521 gatcaggaag aacgacgtga tgctcgtcat ctcccagaag acgaacagca ggaacaggtc
     2581 gtccgccgtg acgagcccga gcatcgacgc ggcgaacagc gtgaacagcc cgtagaaccg
     2641 accgtgccgt ccctcacgct tcaggtaccg cgcggagtag gcggcgacga gcgcgccgac
     2701 gccgaggacg agcaacccga acaggagcga gagcccgtcg aggcggaacg cgaggcggat
     2761 cccgaggccc gggacccacg ggacggccgc cgcgagctcg gctccatcgg ccacgcgcgg
     2821 cgccgccgtg aggagcgcac cggttccggc cgcgtaggcc accgcgatcg cgtagccggc
     2881 gctgcggccc caccatcgcg cggcgagtgg cgcgaccgcg gcgacgacga gcatggccgc
     2941 ggggaggatc agcgggaggg ttgccatccc gtctttacct acacggttat gcgggcgttc
     3001 gcacctttca gccgcgctcg gcgagcagcg gcctcgcgag gtagaccgcg gccacgacga
     3061 ggtagaagag gccgagcagc gccacgcccg gccggaagtc cagcgggagc agcgccgcgg
     3121 cgaacgcgcc gcccacccac gcgagctgga acgagatctc gtagcggacg aagacccgcc
     3181 cgtgcgcctc gcccggggcg tgccgctgca tcaggctcgc gaacgcgagc tggccgaagc
     3241 gggtcgcgag cccggccatc cccgtgaaca gcgccatcgt cgggagcgag aacgtcgtga
     3301 aggcgagcag ggccgctccc ccggcccccg ccagcgcgac gagcacgatc agctcctcgc
     3361 ggatcccgcg cggcaggcgc ggcgcgagga tgtccccgag gtagaccccg gcgaccccgg
     3421 cgatcatgag gaccccgatc cagccggccg ggttgtcggc ccgccggagc gcgaacgcca
     3481 ccaggaacag caggaacccg tgggcccccc gcagccc
//
LOCUS       DATLGG010000154         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_69289, whole genome shotgun sequence.
ACCESSION   DATLGG010000154 DATLGG010000000
VERSION     DATLGG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69289"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VM638_04370"
     CDS             complement(<1..576)
                     /locus_tag="VM638_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HVM11694.1"
                     /translation="MAEIWTIVEHGNGEVTKPSLQALTAARTLAPALGHETAAVFLGT
                     GYDAVKERLGAYGAGKVYVAESDDLESYLVQPAVDVLAKLVEERQPAAILLPSVPSAK
                     DVAAALGAKLGAGVLADVVGIETEGDRLVCTMAVFGGAVNTRCRVKGSPQIVLVKPNS
                     FVAEEAPGELTEERVEVSVSDDAKRAQVIERV"
     gene            complement(576..1361)
                     /locus_tag="VM638_04375"
     CDS             complement(576..1361)
                     /locus_tag="VM638_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HVM11695.1"
                     /translation="MNIVVCVKRVPDTAAEKKLDPNDFTLDRDSVESIMNPVDEYAVE
                     EALKLRDGSGGGEVTILTMGPEGADKNAVRKALALGADKGMLVTDDRLHGSDAMATAY
                     VLALALAQVDFDLCLLGSESTDARTSLVPQALAQLMGMTALTDAKKLEVADGSVTVHR
                     EEEGGYAVVRTDLPAIVSVVKGINEPRYATLKGIMGAKQKEVQVLSGDDLGVDPEMVG
                     LDGSKTEVIEATPRPPKEQGELVKDEGDGHVKLADFLQRNRFV"
     gene            1501..>3293
                     /locus_tag="VM638_04380"
     CDS             1501..>3293
                     /locus_tag="VM638_04380"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HVM11696.1"
                     /translation="MRSRIATVLALLCLLPAGRAAVAQEAEDPWERFLQDRPAFFEAL
                     APPFVECFKRRDSAIDPLSPIFHGCLDWHSAAHAAYSHHVVYHRTGDGTFLTLAEDQI
                     APGGVSLIPAEFQYQRAKGLDLPLTENPYGFGWFLQLVREREISTGKKDFREMADHAA
                     AQMVNWFRARAILGDANGFIQNEAHSNYSWSLINLDVWARHTGDPLLRAAVRDATAPL
                     FNPSLDTSCPVSRDRQTNRTGFQPACLMRLVAVGHVWGEQARDWVMARLPEEGGLNIP
                     RVTTPANCHAGGLNFSRAFALYQLYRITGETAYRDNYADLVRYHVGRPDLYIDPSYAG
                     NPGYLCYSHWVAQFGVRAISLSYEEAPGTPPEILPAGQTSVSITGAQRDGDLLRVNGA
                     AAFAHQPFVTLGTDPSGDTQVPGAAGQDLTSASASTTLDGRLRLRFGVASLPSVTNRG
                     PRTSYSWAFCADASCYEIDAWSTGGSDVEPEVEVWRCATPACTPAGQTLTTIDATADF
                     NGAGRSVTVHVLLDDLGIAPGASITPASPAGSPVFTSMGTRHGPGDSLSYSAAYEAAD
                     RLVSLAVGPPGQDPAVVEYVAPAEIDGAGGYT"
BASE COUNT          464 a         1224 c         1109 g          496 t
ORIGIN      
        1 gacgcgctcg atgacctggg cgcgcttcgc gtcgtcggac accgagacct ccacgcgctc
       61 ttcggtcagc tcgcccggcg cctcctcggc gacgaacgag ttcggcttga cgaggacgat
      121 ctgcggtgag cccttcacgc ggcacctggt gttcacggcg ccaccgaaga cggccatcgt
      181 gcagacgaga cggtcaccct cggtctcgat gccgacgacg tcggcgagga cgcccgcccc
      241 gagcttcgcg ccgagcgccg ccgcgacgtc cttcgccgac ggcacgctcg gcaggaggat
      301 cgccgccggc tgccgctcct caacgagctt cgccagcacg tcgaccgcgg gctggacgag
      361 gtacgactcg agatcgtcgg actccgcgac gtagaccttg cccgcgccgt acgcgccgag
      421 gcgctccttc accgcgtcgt agccggtgcc gaggaagacc gcggccgtct cgtgtccgag
      481 cgccggggcc agggtccgtg cagccgtcag cgcctggagc gagggcttcg tgacctcacc
      541 gttgccgtgc tcgacgatcg tccagatctc agccatcaca cgaacctgtt ccgctggagg
      601 aagtcggcca gcttcacgtg gccgtcccct tcatccttca cgagctcgcc ctgctccttc
      661 ggcgggcgcg gcgtcgcctc gatcacctcg gtcttcgacc cgtcgagccc gaccatctcc
      721 gggtccaccc cgaggtcgtc gcccgagagg acctggacct ccttctgctt cgcccccatg
      781 atccccttga gggtcgcgta ccgcggctcg ttgatcccct tcacgaccga gacgatcgcg
      841 ggcaggtccg tccggacgac cgcgtagccg ccctcctcct cgcggtggac ggtcacggaa
      901 ccgtcggcga cctcgagctt cttcgcatcg gtgagcgcgg tcatgcccat gagctgagcg
      961 agggcctggg ggacgagcga ggtccgggcg tcggtggact ccgaaccgag gaggcagagg
     1021 tcgaagtcga cctgcgcgag cgcgagcgcg aggacgtagg ccgtggccat cgcgtcggac
     1081 ccgtggaggc ggtcgtcggt gacgagcatg cccttgtcgg cgccgagcgc gagcgccttg
     1141 cggaccgcgt tcttgtcggc gccctcgggg cccatcgtga ggatcgtgac ctcgccgccg
     1201 cccgacccgt cgcggagctt cagagcctcc tcgacggcgt actcgtcgac cgggttcatg
     1261 atcgactcga ccgaatcacg gtcgagcgtg aagtcgttcg ggtcgagctt cttctcggcg
     1321 gccgtgtcgg gcacgcgctt cacacagacg acgatgttca caggctcggt ctcctcggaa
     1381 ggccgggcca gacgcccggc gcgctggatt ctagcggacc cgcgggcgga gggaccaccc
     1441 atcccccggg ttgtgcagtt gcacgaagga ggagcgtccc ggggcggcga atccatcggc
     1501 atgcgctcgc ggatcgcaac ggtgctcgcc ctgctctgcc tgctccccgc gggccgggcc
     1561 gccgtcgccc aggaggcgga agacccctgg gagcgcttcc tgcaagaccg tccggcgttc
     1621 ttcgaggcgc tcgcgccgcc gttcgtcgag tgcttcaagc gtcgcgattc ggcgatcgac
     1681 ccgctgagcc cgatcttcca cggctgcctc gactggcact cggcggcgca cgctgcctac
     1741 tcgcaccacg tcgtctacca ccgcaccggc gacgggacct tcctcacgct cgccgaagat
     1801 cagatcgcgc cgggaggcgt ctcgctgatc cccgcggagt tccagtacca gcgggcgaag
     1861 ggcctcgacc tgccgctcac cgagaacccg tacgggttcg ggtggttcct ccagctcgtg
     1921 cgcgagcggg agatctccac ggggaagaag gacttccgcg agatggccga ccacgccgcc
     1981 gcgcagatgg tcaactggtt ccgggcgcga gcgatcctcg gggacgccaa tggcttcatc
     2041 cagaacgagg cccactcgaa ctacagctgg tccctgatca acctcgacgt gtgggcccgt
     2101 cacaccggcg acccgctgct ccgcgcggcg gtccgtgacg cgacggcgcc gctgttcaac
     2161 ccgtcgctcg acacgagctg cccggtttcc cgggaccgcc agacgaaccg caccgggttc
     2221 cagccggcct gcctgatgcg cctcgtggcc gtgggccacg tctggggcga gcaggcgcgg
     2281 gactgggtga tggcgcggct cccggaggaa ggggggctga acatcccccg cgtgacgaca
     2341 ccagcgaact gccacgccgg cggcctgaac ttctcgcggg cgttcgcgct ctaccagctc
     2401 taccggatca ccggcgagac cgcgtaccgc gacaactacg ccgacctggt tcggtaccac
     2461 gtcggcaggc cggacctcta catcgatccc agctacgccg gcaaccccgg ctacctctgc
     2521 tactcgcact gggtggcgca gttcggtgtc cgagcgatct cgctgtcgta cgaggaggcc
     2581 ccgggaacac ctcccgagat cctccccgcg gggcagacct cggtctcgat caccggggcg
     2641 cagcgcgacg gcgacctgct gcgggtgaac ggcgcggccg cgttcgcgca ccagccgttc
     2701 gtcacgctcg gcaccgaccc cagcggggac acgcaggtcc ccggggcggc gggccaagac
     2761 ctcacatccg cctccgcctc gacgacgctc gacggcaggc tccggctccg gttcggtgtc
     2821 gcgtcgctcc cgagcgtgac gaaccgcgga ccgcggacca gctactcgtg ggccttctgc
     2881 gcggacgcct cctgctacga gatcgacgcc tggtcgaccg gcggcagcga cgtcgagccc
     2941 gaggtggagg tctggcgctg cgcgaccccg gcctgcacgc ccgccgggca gacgctgacg
     3001 acgatcgacg cgacggcgga cttcaacggg gccggccgca gcgtgaccgt acacgtgctc
     3061 ctcgacgatc tcggcatcgc tccgggagcc tcgatcacgc ccgcctcgcc cgccggctcc
     3121 cccgtcttca cgtcgatggg gacccggcac ggccccgggg actccctctc gtactccgcc
     3181 gcgtacgagg ccgccgaccg gctggtctcg ctcgcggtcg ggccgccggg tcaggatccc
     3241 gcggtggtcg agtacgtcgc gccggccgag atcgacgggg cggggggcta cac
//
LOCUS       DATLGG010000155        19862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_100951, whole genome shotgun sequence.
ACCESSION   DATLGG010000155 DATLGG010000000
VERSION     DATLGG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100951"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VM638_04385"
     CDS             complement(<1..1813)
                     /locus_tag="VM638_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HVM11697.1"
                     /translation="MLPGGIRALLTLSLVTLVTLTTLPTHGSAPAERPRPGAAAEIAL
                     DHLRAHHERYGVGAADVADLRVQDALVSPHTGVTHVYIRQRHQGLDVLGGDATVNVGP
                     DGPVIHVGSRLVAGLASASGTARLDAPAAVRAAASALGLRPDASLRVARGPAPGGERA
                     TVLTRGGISLSPIPVRLAYQPSPQGIRLAWNLEIEEISEEHWWNVSVDASTGAVLAEI
                     DYVAHDRGDEIAAATGRVTGRRLAPLAAGSGRGNDGARYRVFALPLESPNDGDRTLVT
                     SPADALASPFGWHDTNGAAGPEFTTTQGNNVHAYLDTTNTATTGVVPPGLDAEGGPTL
                     TFDFPWNPDLPPTATKDAAVTNLFYWNNVIHDVFYRYGFTEAAGNFQVNNYGRGGTGN
                     DSVRAEAQDGSGTNNANFATPADGSRPRMQMYVWTTGLQSQRRIIDGDMDAGVIIHEY
                     GHGISNRLTGGRNNVGCLNNQEQMGEGWSDWLAIALTALPTETGAQRRGMGTYVLAQP
                     NRQGSGIRPTPYSTNMSINGATYDTIKTAAVPHGVGYVWSTMLWEVFWDLVDEHGFNP
                     NVYEDWTTGGNNLAIQLVMDGMKMQPCSPGFVDGRNAI"
     gene            complement(1964..2443)
                     /locus_tag="VM638_04390"
     CDS             complement(1964..2443)
                     /locus_tag="VM638_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVM11698.1"
                     /translation="MDDREKETTMNDTSWIRGVIDGMAMDEPVELATEEAEGWLILKG
                     NVPERWRDRAVPLYLVPLLPQEAEQVVAGSTLLPDVHLEEEPLLRLVARGRKTASIAR
                     ELGIPLRTIERRLAKLRERFNVASTADLTILLSRLGFGHESAPTDPRPPVAPTPLHR"
     gene            2572..3666
                     /locus_tag="VM638_04395"
     CDS             2572..3666
                     /locus_tag="VM638_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM11699.1"
                     /translation="MLAEQALSHRQDRFESLYRRYYWSLVGFCRGSLRSRAAAEDAAQ
                     EAFFRAYRYLEALDPSRSAWPWLKAIARNVIADRARRDARETPVEAPEAAPITNTDRA
                     ESSLALTEAMSTLPPRHRLALYLRYVEGLDPTAAAQRLDVSIAAFNQLLFRARSRLKA
                     ELRRLGEAVPALLAWPAHWMRGRAQRLSARAAGRSPVAVTRFAPEILLQVASGFIAVA
                     VIFGGGGWRAASAGESQAEVVAQLAASNVALGSPVGDSPRAERTRAWESARLAAVAQS
                     THGAATPSGGGGGKAGGSIEVVKKVQEVASDATRKARFRVSLPGVGLPDPAPVVEKAK
                     GVVCSVACGGPSAPVIVEPPSLPLDLPVEP"
     gene            complement(3806..4579)
                     /locus_tag="VM638_04400"
     CDS             complement(3806..4579)
                     /locus_tag="VM638_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM11700.1"
                     /translation="MPEGSISFDRAARYYDRTRRVSPQLLRAMVDLLRAEAPPRGLAL
                     EVGTGTGTLSLPLHRKGSRVVGLDLSLEMLRQVRAKGEPGPPLVGGDAMLLPFRNGTF
                     DLVFARHVLHLVGEWRTAAGEMARVAARGGRILVTGGCYAGAYDELIEVFTREAGRAR
                     PLPGLLPSEEPLLDEWMRSLGARVRVLDLGSEPPDETLREFLAAIDANLFSWTWPLGE
                     RDRERAAAATRRWAEERYGDLDRPIPAQLRVIWRAYDLP"
     gene            complement(4597..5082)
                     /locus_tag="VM638_04405"
     CDS             complement(4597..5082)
                     /locus_tag="VM638_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVM11701.1"
                     /translation="MERLNGDADLRVRRLGSSAGIGLSGEIDLSTVDLLRGSLAREVA
                     RGGRVVLDLREVTFMDSTGLKAIVQASRQLEGRGTLEILPSPVVRKLFDLTGMGQAPT
                     IMVVPDAVEDPDHEVDPTLARRLQDPVFEGAYRSWLASVTRGVRNRGRRGPTGADHSL
                     N"
     gene            5226..5681
                     /gene="tadA"
                     /locus_tag="VM638_04410"
     CDS             5226..5681
                     /gene="tadA"
                     /locus_tag="VM638_04410"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HVM11702.1"
                     /translation="MHEKMMGLALEEARTCIGWGDVPVGAVVAKGTEILARAGNERER
                     RGDPTAHAEILALREAAARAGSWRLDGCTLYVTLEPCAMCAGAVVLARLDRLVFAAKD
                     PKAGFAGSLGDLVRDPRLNHRAEVVTGVLAEEAGELLRSFFRDRRAPAG"
     gene            5695..5784
                     /locus_tag="VM638_04415"
     tRNA            5695..5784
                     /locus_tag="VM638_04415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5729..5731,aa:Ser,seq:cga)
     gene            5838..5929
                     /locus_tag="VM638_04420"
     tRNA            5838..5929
                     /locus_tag="VM638_04420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5872..5874,aa:Ser,seq:gga)
     gene            5976..6244
                     /gene="ffs"
                     /locus_tag="VM638_04425"
     ncRNA           5976..6244
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VM638_04425"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            6281..8026
                     /gene="dnaX"
                     /locus_tag="VM638_04430"
     CDS             6281..8026
                     /gene="dnaX"
                     /locus_tag="VM638_04430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HVM11703.1"
                     /translation="MEEYRSLYRKYRPQAPAEVLGQEHVVRALGGAVREGRLAHAFLF
                     SGPRGTGKTSTARILAKMVNCVNGPTAEPCGVCDQCVHIRDGTHMDVVEIDAASHGGV
                     DDARELRERAPTAPAEGREKVYIIDEAQRLTASAFDALLKLFEEPPPGVRFVLATTEP
                     HKMPPTIAGRCQRFEFRRVQADVIAEQLQRIAAKEGLELAATAAAAIARRSDGSVRDA
                     LSLLDQASVLGGGTVSDEVVVSLVGAPETEVRFALADAIAVGDARDVFGIVHRLVQEG
                     HDLRHFAAQVTAHFRDLLLALSAPEEPGIVDVPAELHPRLVAQAGKFSAGEIDRILSL
                     LLQAQSDMRWSTAPRLTLELALVRAAMPETDPTPAALVSRLERLERLAGIVQEAEPVR
                     AAPTAAAAKEEPVRAAPTTAAAKETPAPARPPTPPAHAPEASPGPQRRAADESPGGSS
                     SSVGAGPPSKVDAVMLRGSWPRVVEALKTRRKMVLFANAQIATVGSYDGEVLELVFPP
                     GRAFGASKVEEKQADLREVLREVFGITPQIRCTVREGALPEPEPDGEPPATPEDAARL
                     LREQFGAEVVEEAES"
     gene            8027..8620
                     /gene="recR"
                     /locus_tag="VM638_04435"
     CDS             8027..8620
                     /gene="recR"
                     /locus_tag="VM638_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HVM11704.1"
                     /translation="MFEGPIQDLVDELNRLPGIGPKSAQRLAFYLVKSPAQDAKRLAE
                     AIVQAKERVHFCRECFNVAEGELCRYCSDPTRDPAIVAVVEEPKDVSAVEKAGVIRGR
                     YHVLGGAISPMDGIGPDDLRVQELLDRVQRAGIKEVILATNPNLEGNATAMYVSGLLK
                     PLGVRVTRLASGLPVGGDLEYADEVTLGQALEGRREM"
     gene            8673..9908
                     /locus_tag="VM638_04440"
     CDS             8673..9908
                     /locus_tag="VM638_04440"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HVM11705.1"
                     /translation="MGIIVQKYGGTSVGDIDRINAVAERVVAARRKGNDVVVVVSAMG
                     ASTDDLLRMAHELTPLPNARELDLLLTAGERIAMSLLAIAINARDVPAASYTGSQAGI
                     ITDTSHGKAKIIDIRPGRIREALDRGAVVIVAGFQGVSTAYDVTTLGRGGSDTTAAAL
                     AAALEADVCEIYTDVAGVYTADPRIVPGARKLHAVSYEEMLEMAASGARVLQLRAVEY
                     ARRYGIRLHVRSSFTHEPGTWIAEEDERMEQAIIAGVTHDESEAKVTVEDVPDRPGVA
                     ARIFRAVAEEGINVDMIVQNVSHGGTTDVSFTIPKSDVPHARKVMEAVVEEVGAARHT
                     TDEEIAKISLVGAGMKTHPGVAADMFSALADAGVNIEMISTSAIRVSCVIRAEDVHRA
                     VEALHARFDLAEDSVAREH"
     gene            9911..10936
                     /locus_tag="VM638_04445"
     CDS             9911..10936
                     /locus_tag="VM638_04445"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVM11706.1"
                     /translation="MAAPRVAVAGATGAVGREILRILEERETPVGTLVPLASARSAGT
                     RLRFRGQEVEVRPLDAQDWSEIDLALFSAGATVSREVAPRAVEAGTIVVDNSSAWRMD
                     PGVPLVVPEINAHALEGHRGIVANPNCTAIVALMALAPLHRAAGLSSVIISSYQSVSG
                     AGHRGVTELLEQVEKLRGQEEDLTHPDLDALPRGEIFGPTIAYNVVPRAGSFEDDGFT
                     SEERKLADESRKILELPGLPVVGTVVRVPVIAGHAVSIHAIFERDLSPAAAWETLHAA
                     EGVRVVDDPASDLFPTPLESAGTDDVLVGRIRRAAERSLLLFAAGDNLRKGAALNAVQ
                     IAERLLG"
     gene            complement(10933..12231)
                     /locus_tag="VM638_04450"
     CDS             complement(10933..12231)
                     /locus_tag="VM638_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM11707.1"
                     /translation="MRANRHDLVTASVMLLLLVWAPAAVAATPTCGGKRATIVGTNGH
                     DTINGTSRNDVIVARGGADVIDGRGGNDRICDGSGNDWVVGDAGNDRFYNGPGADEFS
                     GGAGHDTVSYAGSSVGVVVTIDNMADDGPDSDNVRTDIEGIIGSRFSDHLTAQAGDVP
                     NVLSPGGGNDIVSGEGGDDRIVGGSGSDSIDGGPGDDRLEGGSDPDTILGGLGRDTAY
                     GGGSRDWVIGDADDDQLYGGGSGDRLQGGPGNDTLHGGSGVDIMTGDHVGEPGGEDRL
                     FGGDGADQIFGYGGADTIEGEGGADQLVGGSEDDRLIGGPESDELLGGTGEDRLFGHG
                     GHDQLRGEAGDDVLHGGGGDDSLLGGDGEDEAFGGDGEDDLLGGAQNDLLEGNAGEDV
                     LQGDDGNDELDGGRHWDSLNGGFGVDVCRNGETVYDCEPT"
     gene            complement(12315..13574)
                     /locus_tag="VM638_04455"
     CDS             complement(12315..13574)
                     /locus_tag="VM638_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM11708.1"
                     /translation="MLALTLLLVSPPAQAATPTCLGKAATIVGSNGNNTINGTSGNDV
                     IVAKDGSDSIDGKGGNDRICGGAGSDTVRGGSGSDLLHGGPGADVFHGGTGTDTVTYQ
                     DRTTGVVVRIDNIANDAGESDDVRTDVEDLRGGKGNDSLYAPSADLVANRLTGGPGHD
                     WLEGGPGNDRFDGGAGGDAADGGSGNDRFFGGAGTDSFYAGAGDDVGYGGPDPDQLDG
                     FEGKDRMYGEGGRDSLSGFEDDDLLVGGGGSDYLEGHGGDDVLRGGDGSDHMLGRAGE
                     DELTGGRGWDVMSGNEGKDAMSGGSEGDFLDGGADDDTLSGGDHDDVVYGGAGADTLT
                     GADGDDDLYGHAGADRLFGGAGDDDLYGHEDNDVMRGEGGEDVLYGGDADDHLDGGSR
                     WDELYGDGGFDLCRNGERLNGCEGQWT"
     gene            complement(13646..14884)
                     /locus_tag="VM638_04460"
     CDS             complement(13646..14884)
                     /locus_tag="VM638_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM11709.1"
                     /translation="MQRRARITLTGLISGILLVGLSPAQAATPTCLGKKATIVGTNGD
                     NTINGTAGDDVIVGRGGNDVINGRSGNDVICGGGGNDDMFGGGDNDTLHGGPGGDRFS
                     GGKGKDTATYQGYVTDVGVTLDDNSNDTDEGDNVLSDIENIRGGNGNDYLVPQLEAVA
                     NRLIGGPGNDILYGLGGNDLLEGGDGSDSIRGGLGDDTILGGANPDDLNGQEGNDRVF
                     GEGGRDTITGEDGVDRLEGGAGRDSLTGGEGDDDIRGGTGADTIHGSDGEDQLYGGAG
                     WDVIHGEAGKDTIYGGTEGDYLTGGADNDILHGGDHDDRLEGGSGEDTLLGGRHDDLL
                     YGEIGHDVLDGGEGDDDLEGGPQNDTLFGTNGDDRLLAGAGDDQLDGGRHYDVLNAGE
                     GDDQCRNGELHEDACEMAWT"
     gene            complement(14896..16206)
                     /locus_tag="VM638_04465"
     CDS             complement(14896..16206)
                     /locus_tag="VM638_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM11710.1"
                     /translation="MAAALVTLAASHAHAATPTCGGKTATIVGTSAGQTINGTSGDDV
                     IHGRGGNDVINGKGGNDRICGGDGNDTLNGDAGNDRFYNDAGSDVFSGGGGRDTVSYA
                     RSASLVIVSINDVANDGGEGDDVRTDVEGVVGSRYDDLISGNASSNVLSGGGGDDTIA
                     GFGEADLLDGGSGSDYLEGGEGHDDLFGGPDPDEIEGGAGVDWIFGEGGRDDLRGGGD
                     DDVIFGGDARDSLSGGDGEDTLFGDAGNDAIIADAVAPFPDFVDAGPGRDWVLAGGGD
                     DEVWGGEGEDSIQGGDGSDIIYGGTEGDQLLGEAGSDSILGGGGGDIILGGAANDFLE
                     GGSGYDSITGGDGNDEILGGSGDDDLIGGTGTDELRGNAGGDDLRGDEHNDRLFGGDG
                     DDSIQGGAGDDALDGGRHFDRLDGGDGTLDACTRGEILIGCETV"
     gene            complement(16315..16599)
                     /locus_tag="VM638_04470"
     CDS             complement(16315..16599)
                     /locus_tag="VM638_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HVM11711.1"
                     /translation="MDLRDFQELMRRTYAERDAARGPDATFRWLVEEVGEVARAMRRG
                     DPENLREEVGDALAWLTSLANLLGVDIADAAARYEHGCPKCGGMPCACPL"
     gene            complement(16599..17501)
                     /locus_tag="VM638_04475"
     CDS             complement(16599..17501)
                     /locus_tag="VM638_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVM11712.1"
                     /translation="MRTAPVISTAPGTRREAFGPVEWGLLSAIALMWGSSFVFIEWGL
                     QGLQPGLVAFLRIALGAASVAVIPRARRPVAREDWPRIALLGAIWMAIPLTLFPVAQQ
                     WITSSLAGMINGGMPLFAGLFAAILLRRMPRPVQVAGLLVGFAGVVLLTSPGTREGSA
                     ALLGAVLVLFATMLYGLSANLTVPLQQRYGALPIVLRAQLSAVVLLLPVGLLSLRGST
                     LDASSLASTAVLGVFGSGMAFLAMATLVGRAGATRGAVAIYFLPVVATILGVMLLDEQ
                     VDPLQLVGMGLVLAGAWLTSRRER"
     gene            17538..17918
                     /locus_tag="VM638_04480"
     CDS             17538..17918
                     /locus_tag="VM638_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVM11713.1"
                     /translation="MRRLESVILYVEDLDRSIRFYGDVLGLDLALSEHGYAEFRLENV
                     KLALYERERAVRLTGAERPPAGAAEILFVVEDADRWAERLADHVLAGPVDHPWGHRSV
                     HVADPDGHVVELAQEIPRTRPRGA"
     gene            complement(17950..18026)
                     /locus_tag="VM638_04485"
     tRNA            complement(17950..18026)
                     /locus_tag="VM638_04485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17990..17992),aa:Pro,seq:cgg)
     gene            complement(18075..>19862)
                     /locus_tag="VM638_04490"
     CDS             complement(18075..>19862)
                     /locus_tag="VM638_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM11714.1"
                     /translation="IVTLAIDAATGEVRWTNRRDGGGNAFDAGAALALSPDARTLFVG
                     GTAVVGSATSSPRYALQALDAATGAERWFSSYASPASTGQSFGADLVVDERGDRVFVT
                     GRSEAGPAERFDIATVAFEATDGALAWDARYAFPGHLDEGGSAIDYDPDTDRVYIAGW
                     SGTFLPAQSRYVALGYDAGSGHEVWSARYGMRTDDAVRLTDEDRAAAIAIDSASQRLY
                     VAGTLGRRDSVGSTTVHRTFGTLAYAMPKAGRRALPDAGGWRSPNLEFVAHLATNNDA
                     VGARIVGNLMYLTTSQHLKIYDISEPADPQLVGLLAIPAEEAPYLGEEDVDTNGKILL
                     FGRKVVDVSDPKNPTLREGNLPANPHTWSCVLNCTWAYGSEGQIVNLANHTVLAGNWR
                     TGKPATNNLHDVTEISPGLVVTSSNPFLLLDARENPANPRLVARGPSESRFNHGNLWP
                     NGGTDRFLLVGGESTGPTANCSANGSAVLRTFDVSRWAEDGTIAQTGSYTVRNGLFTD
                     GDAPADLFCAHWFDTHPTYRNGGVVAMAWYEHGTRLLRVSKEGRVREIGYYVPYGGST
                     SAAYWAGDDILYSVDYNRGVDILRYTGRG"
BASE COUNT         2720 a         7142 c         6929 g         3071 t
ORIGIN      
        1 ggatcgcgtt ccgcccgtcg acgaagcccg ggctgcacgg ctgcatcttc atcccatcca
       61 tgaccagctg gatcgccagg ttgttcccgc cggtggtcca gtcctcgtag acgttcgggt
      121 tgaacccgtg ctcgtccacg aggtcccaga agacctccca gagcatcgtc gaccagacgt
      181 agccgacccc gtgcggaacg gcggcggtct tgatcgtgtc gtaggtcgcg ccgttgatcg
      241 acatgttcgt cgagtacggg gtgggacgga tcccgctgcc ctgccggttc ggctgagcca
      301 ggacgtaggt ccccatcccg cgccgctgcg cccccgtctc ggtgggcaac gcggtcagcg
      361 cgatcgcgag ccagtcgctc cagccctcgc ccatctgctc ctggttgttg aggcacccga
      421 cgttgttccg cccgccggtg aggcggttgg agatcccgtg tccgtactcg tggatgatca
      481 cccccgcgtc catgtcgccg tcgatgatgc gccgctgcga ctggagcccg gtcgtccaca
      541 cgtacatctg catccgcggc cggcttccgt cggcgggcgt ggcgaagttc gcgttgttgg
      601 tcccgcttcc atcctgcgct tcggcccgca ccgagtcgtt cccggtcccg ccgcgcccgt
      661 agttgttcac ctggaagttc ccggcggcct cggtgaagcc gtagcggtag aagacgtcgt
      721 ggatgacgtt gttccagtag aagaggttcg tgacggcggc atccttggtg gccgttggcg
      781 ggaggtccgg gttccagggg aagtcgaagg tcagggtcgg gccgccctcg gcgtcgaggc
      841 cgggcggcac gaccccggtg gtcgcggtgt tcgtggtgtc gaggtacgcg tggacgttgt
      901 tgccctgcgt ggtggtgaac tccggccctg cggccccgtt cgtgtcgtgc cagccgaacg
      961 gggaggcgag agcgtcggcg gggctcgtga cgagcgtgcg gtccccgtcg ttcggagact
     1021 cgagcggaag ggcgaagacg cggtagcggg cgccgtcgtt cccacgcccc gatcccgccg
     1081 cgagtggggc gaggcgcctc ccggtcacgc gccccgtcgc ggcggcgatc tcatcaccgc
     1141 ggtcgtgggc gacgtagtcg atctcggcga ggaccgcccc ggtcgaggcg tcgacgctca
     1201 cgttccacca gtgctcctcc gagatctctt cgatctcgag gttccatgcg agccggatgc
     1261 cctgcggcga gggctggtac gcgaggcgta ccgggatcgg cgacagcgag atcccgccgc
     1321 gcgtgagcac cgtcgcgcgc tcgcccccgg gtgcgggtcc ccgcgccacg cgcagggagg
     1381 catccggccg caggcccagt gcgctcgccg ccgcccggac cgcggcggga gcatccagcc
     1441 gcgcggttcc ggaagccgag gccagtcccg ccacgaggcg ggagcccacg tggatgacgg
     1501 gtccgtcggg cccgacgttc accgtggcat cgccgccgag gacgtcgagc ccctggtgcc
     1561 gctgccggat gtagacgtgg gtcacgccgg tgtggggtga gacgagcgcg tcctgcacgc
     1621 gcagatccgc cacgtcggcc gcgccgaccc cgtaccgctc gtggtgcgcg cggaggtgat
     1681 cgagggcgat ctcggcggct gcgcccggac ggggccgctc ggcgggtgcc gagccgtgcg
     1741 tgggtagcgt ggtgagcgtg accaacgtga ccagcgagag cgtgagcaac gctcggatgc
     1801 ctcccggcag catggatccc ccccttgtcc ggcccctgcg ggcgtggcct accgggaggg
     1861 ttctcgaaca gccgcgcgat gtcctgtgtg caactgcaca aacctctcgg acatgcggaa
     1921 ggccccggcc atgagctcgg ccggggcctt ccgacggggt ggctcagcgg tgcagcggcg
     1981 tgggagcgac cggcggccgc gggtcggtcg gagcgctctc gtgcccgaac ccgagccgcg
     2041 agagcaggat cgtcaggtcc gccgtcgagg cgacgttgaa ccgctcgcgc agcttcgcga
     2101 ggcgccgctc gatcgtccgg agcgggatcc cgagctcgcg tgcgatcgag gcggtcttgc
     2161 ggccccgagc gaccagcctg agcaggggct cctcctcgag gtgcacgtcc ggcaggagcg
     2221 tgctgccggc gaccacctgc tccgcttcct gcgggaggag cgggaccaga tagagcggca
     2281 ccgcgcggtc ccgccagcgt tccggaacgt tgcccttgag gatcagccag ccttcggctt
     2341 cctcggtcgc gagctcgacc ggctcgtcca tcgccatgcc gtcgatcaca ccgcggatcc
     2401 agctggtgtc gttcatcgtc gtctccttct ctcgatcgtc catgtccccc caaataccgg
     2461 gcaggggggg ctgcgataac gggggcgacg gaaaaaatct ttcggcagga gattcggtcg
     2521 aaacggcgaa cacataggcc agcagggggg gaggacgaaa ggggaagggc tatgctcgcc
     2581 gagcaggcac tctcgcaccg ccaggatcgc ttcgaatccc tctatcggcg ctactactgg
     2641 agcctcgtcg gcttctgccg cggatcgctc cggagccgcg ctgccgccga ggacgccgcg
     2701 caggaggcct tcttccgcgc gtaccggtac ctcgaggcgc tcgacccgtc gcggtcggcc
     2761 tggccgtggc tcaaggcgat cgctcggaac gtgatcgccg accgggcccg tcgcgacgct
     2821 cgggagaccc cggtggaggc ccccgaggcc gcgcccatca cgaacaccga ccgggcggag
     2881 agctcgctcg ccctcacgga ggcgatgtcc acgctccctc cccgtcaccg gctggcgctc
     2941 tacctccgct acgtcgaggg gctggacccc acggccgccg ctcagcggct cgacgtcagc
     3001 atcgccgcct tcaaccagct gctgttccgc gctcgctccc ggctgaaggc cgagctgcgg
     3061 cggctgggcg aggccgtccc cgctctgctg gcctggccgg cccactggat gcgcggccgc
     3121 gcacagcgtc tttcggcccg ggccgccggt cgctcccccg tcgcggtcac ccgattcgcc
     3181 ccggagatcc tcttgcaggt cgcgagcggc ttcatcgcgg tggcggtcat cttcggtggt
     3241 gggggatggc gcgccgcttc ggcaggggag agccaggccg aggtcgtggc gcagctcgcg
     3301 gcatcgaacg tcgccctggg aagcccggtt ggcgatagcc ctcgggcgga gcgcacgcgc
     3361 gcctgggagt cggcacgcct ggcggccgtg gcacagtcga cccacggcgc tgcgaccccc
     3421 tccgggggcg gcggcggcaa ggccggcggc tccatcgagg tcgtgaagaa ggttcaggag
     3481 gtcgcgagcg acgcgacccg caaggctcgc ttcagggtca gcctccctgg cgtaggcctg
     3541 cccgacccgg ctccggtggt cgagaaggcg aagggggtcg tctgctcggt cgcttgcggc
     3601 ggcccgagcg cgcccgtgat cgtggaaccg ccctcgctcc cgctcgatct gccggtcgag
     3661 ccctaggcaa cgtcaagaac caggaggagg gccgcgcggt aagcaggggg gtgcgtcccg
     3721 tcgcggccct ctcctggttc acccttcgat accggtcgga ggccgctgcg ataacggaga
     3781 tcgtcgagat cttcggacgg ccccctcagg ggaggtcgta ggctcgccag atcacccgga
     3841 gctgcgcggg gatcgggcgg tcgaggtcgc cgtaccgctc ctccgcccag cgccgggttg
     3901 ccgccgcggc tcgttcgcgg tcccgctcgc ctagcggcca ggtccacgag aagaggttcg
     3961 cgtcgatcgc cgcgaggaac tcgcgcaggg tctcgtccgg cggctcggac ccgagatcca
     4021 ggacgcgcac gcgcgccccg agggagcgca tccactcgtc cagcagaggc tcctcggatg
     4081 gcaggagccc ggggagcgga cgcgcgcgac ccgcctctcg cgtgaagacc tcgatcagct
     4141 cgtcgtaggc gccggcgtaa cagcctccgg tgacgaggat ccggccaccc cgggcggcca
     4201 cccgagccat ctcgcccgcg gcggttcgcc attccccgac gaggtgcagg acgtgccggg
     4261 cgaagaccag atcgaaggtc ccgttccgga aggggagcag catggcgtca ccgccgacga
     4321 gcggtggccc cggctcgcct ttcgctcgga cctgccggag catctcgagc gagaggtcca
     4381 gccccacgac ccggctcccc ttccggtgca gcgggaggga cagcgtccct gtgccggtcc
     4441 cgacctcgag cgccagtccg cgaggcggag cctcggcacg gaggagatcg accatcgcgc
     4501 ggaggagctg gggggagacg cggcgcgtgc ggtcgtagta ccgggcggcg cggtcgaacg
     4561 agatcgatcc ctccggcacc gcccgaggat acgccgctag ttgaggctgt ggtccgcgcc
     4621 ggtcggcccc cgtcggccgc ggttgcggac gcccctcgtc acgctcgcga gccacgagcg
     4681 gtacgccccc tcgaacaccg gatcctgcag gcggcgggcg agcgtcgggt cgacctcgtg
     4741 gtcggggtcc tcgacagcat ccggcacgac catgatcgtc ggagcctggc ccatgccggt
     4801 gaggtcgaag agcttcctga cgaccggcga cggcaggatc tccagcgttc cgcgtccctc
     4861 cagctgtcgg gaggcctgga cgatcgcctt gagccccgtg ctgtccatga aggtcacctc
     4921 tcgcaggtcg aggaccactc ggcctccgcg ggccacctcc cgggccagcg atccccggag
     4981 caggtcgacc gtggaaagat cgatctcccc gctcagcccg atccccgcac ttgatccgag
     5041 cctgcgcacg cggaggtccg cgtcaccgtt gagcctctcc atgcgctgct ccctctgtcg
     5101 tgctcgcctg ctggggtgtc cggcgaacgt ctgttcggta ccgaacctac caactggggg
     5161 cgaaaccgtc ggtccgggag cgtacgccgg ggtcggccac ccccatgcgg tagcgttgtc
     5221 ccgagatgca cgagaagatg atggggctgg cgctggagga agcgcggacc tgcatcggat
     5281 ggggcgacgt ccccgtcggc gcggtggtcg cgaaggggac ggagatcctg gcacgcgccg
     5341 gcaacgagcg ggagcgacgc ggcgacccca cggcgcacgc cgagatcctc gccctccgcg
     5401 aggcggcggc gcgggcaggg tcgtggcgcc tcgacggctg caccctctac gtgaccctcg
     5461 agccgtgcgc gatgtgcgcg ggcgcggtcg tcctcgcgcg cctcgaccgg ctcgtcttcg
     5521 cggccaagga cccgaaggcc ggtttcgcgg gttccctcgg agacctcgtc cgggaccccc
     5581 ggctcaacca ccgcgcagag gtcgtgaccg gcgtcctggc ggaggaggca ggcgagctcc
     5641 tgcgctcctt cttccgggat cggcgcgcac cggccggctg atacactcgc cgacggaggc
     5701 gtgccggagc ggccgaacgg gaccgcctcg aaagcggtta ggggttcacg cccctcgtgc
     5761 gttcaaatcg caccgcctcc gccagtcgtg acgcgggcgg cccctcgtgg gtcgcctgcg
     5821 ctacgatcgt cgagcttgga ggcatgccgg agcggccgaa cgggcgcgcc tggagagcgc
     5881 gtaggtgggg gaacccgcct cgaaggttca aatccttctg cctccgccaa ccgccgaagc
     5941 ccgtgtcggg cccggggtct atactggcct cgtgaccgtg ctaggcgggg agttagcggt
     6001 gccctgtcac tcgcaatccg ctctagcgag gccgaagatc cgtctgcggc ccgagcggtc
     6061 cggaagagcc tcgtcgagac gcggcgtcga tggccgggtc ccgcgcgacg tcggtccgtg
     6121 aaccccgtca ggcccggaag ggagcagcgg taagcgggag ccggcgggtg ccgcgggcgc
     6181 gcctggtcgg agcctgcgct ccgggaagcg tccgggccgt ctggggtcaa gggcggatgc
     6241 acggtcacct ttcttcgatc cttccccggc gaggatgaac gtggaggaat accgctccct
     6301 ctatcggaag taccgccccc aggcacccgc cgaggtcctc gggcaggagc acgtcgtccg
     6361 ggcgctcggc ggcgccgtcc gggagggccg gttggcgcac gcgttcctct tctcgggccc
     6421 gcgcggcacc gggaagacct cgacggcgcg gatcctcgcg aagatggtca actgcgtgaa
     6481 cggccccacc gccgagccct gcggcgtctg cgaccagtgc gtccacatcc gcgacggcac
     6541 ccacatggac gtcgtcgaga tcgacgccgc gtcccacggg ggggtggacg acgcgcggga
     6601 gctccgcgag cgagcgccca ccgcgccggc ggagggccgg gagaaggtct acatcatcga
     6661 cgaggcgcag cgcctgacgg cctccgcctt cgacgccctg ctgaagctct tcgaggagcc
     6721 gccgccgggc gtgcggttcg tgctggcgac gaccgagccc cacaagatgc cgccgacgat
     6781 cgccgggcgc tgccagcggt tcgagttccg acgcgtgcag gccgacgtca tcgccgagca
     6841 gctccagcgg atcgcggcga aggagggact cgagcttgcc gccaccgcgg cggccgccat
     6901 cgcgcgccgg tcggacggat ccgtccggga cgcgctctcg ttgctcgacc aggcgtcggt
     6961 gctcggcggg gggacggtca gcgacgaggt cgtggtctcg ctggtcggag ccccggagac
     7021 ggaggtgcgg ttcgcgctcg ccgacgcgat cgccgtcggg gacgcgcggg acgtcttcgg
     7081 gatcgtgcac cggctcgtcc aggaggggca cgacctgcgg cacttcgccg cccaagtgac
     7141 cgcgcacttc cgcgacctcc tgctcgcgct gtcggctccc gaggagccag ggatcgtcga
     7201 cgtgccggcc gagctgcatc cgcgcctcgt cgcgcaggcc gggaagttct cggccgggga
     7261 gatcgaccgt atcctctcgc tgctgctcca ggcgcagagc gacatgcgct ggtcgaccgc
     7321 gccacgtctc acgctcgagc tcgcgctcgt ccgcgccgcg atgccggaga ccgatccgac
     7381 gccggcagcg ctcgtctcgc gcctcgagcg cctcgagcgg ctcgccggga tcgtgcagga
     7441 ggcggagccc gtccgcgcgg cgcccacggc cgcggccgcg aaggaggagc ccgtccgcgc
     7501 ggcgcccacg accgcggccg cgaaggagac gcccgccccc gcccggccac cgacgccgcc
     7561 ggcgcatgcc cccgaggcga gcccgggccc acagcgtcga gccgcggacg agagccccgg
     7621 tgggtcctcg tcatcggtgg gcgccggtcc accttcgaag gtcgacgccg tgatgctgcg
     7681 cgggtcgtgg ccgagggtgg tcgaggcgct gaagacgcgg cggaagatgg tgctgttcgc
     7741 gaacgcgcag atcgccaccg tcggctcgta cgacggagag gttctcgagc tggtcttccc
     7801 tccgggccgt gcgttcgggg cgtccaaggt ggaggagaag caggccgacc tgcgggaggt
     7861 cctccgtgag gtcttcggca tcacgccgca gatccgatgc acggtgcggg agggcgcgct
     7921 tcccgagccg gagcccgacg gcgagccgcc ggcgacgccg gaggacgccg cgcgcctcct
     7981 gcgggagcag ttcggggccg aggtcgtcga ggaggcagag agctaggtgt tcgagggccc
     8041 gatccaggac ctcgtcgacg agctgaaccg gcttccgggc atcgggccga agtcggcgca
     8101 gcgcctcgcc ttctacctgg tcaagtcgcc ggcacaggat gcgaagcgcc tcgccgaggc
     8161 gatcgtgcag gcgaaggaac gggtgcactt ctgtcgcgag tgcttcaacg tcgccgaggg
     8221 tgagctctgc cgttactgct cggacccgac gcgggacccg gccatcgtgg cggtcgtcga
     8281 ggagccgaag gatgtctcgg cggtcgagaa ggcgggcgtg atccgcggcc gctaccacgt
     8341 cctcggtggc gccatctcgc cgatggacgg gatcgggccc gacgacctcc gggtccagga
     8401 gctcctcgac cgcgttcagc gcgcggggat caaggaggtc atcctcgcga cgaaccccaa
     8461 cctcgagggg aacgcgaccg cgatgtacgt gagcggcctg ctcaagccgc tcggcgtccg
     8521 ggtgacgcgc ctcgcctcag ggcttcccgt cggaggagac ctcgaatacg ccgacgaggt
     8581 gaccctcggt caggccctgg aaggccgccg cgagatgtag ccgtcctccg cggcggcctc
     8641 ggagcggctg atagcctcgg ggcggaggag ccatgggcat catcgtgcag aagtacggcg
     8701 gcacgtccgt cggggacatc gaccggatca acgccgtggc cgagcgggtc gtcgcggcca
     8761 ggcggaaggg gaacgacgtc gtcgtggtcg tctcggcgat gggagcctcc accgatgacc
     8821 tcctgcggat ggcgcacgag ctgacacccc tgccgaacgc gcgcgagctc gacctgctcc
     8881 tgacggcagg cgagcggatc gcgatgtcgc tcctcgcgat cgcgatcaac gcccgcgacg
     8941 tgccggccgc ctcctacacc ggctcgcagg ccggcatcat caccgacacg tcccacggga
     9001 aggccaagat catcgacatc cgcccgggcc ggatccgtga ggccctcgac cggggggcgg
     9061 tcgtgatcgt cgccggcttc cagggcgtct ccacggccta cgacgtgacc acgctcggac
     9121 gcggcggatc ggacacgacg gccgcggccc tcgcggcggc gctcgaggcg gatgtctgcg
     9181 agatctacac cgacgtcgcc ggcgtctaca ccgcggaccc gcggatcgtc cccggcgcgc
     9241 gcaagctcca cgcggtctcc tacgaggaga tgctcgagat ggcggcgagc ggagcgcgcg
     9301 tccttcagct gcgtgcggtg gagtacgctc gtcgatacgg catccggctg cacgtccggt
     9361 cctcgttcac ccacgagccc ggaacctgga tcgcggagga agacgagcgt atggagcagg
     9421 cgatcatcgc cggcgtcacc cacgacgaga gcgaggcgaa ggtcacggtc gaggacgtcc
     9481 ccgaccgccc gggcgtggct gcgcgcatct tccgcgcggt cgcggaggag gggatcaacg
     9541 tcgacatgat cgtgcagaac gtctcgcacg gcgggacgac cgacgtctcg ttcacgatcc
     9601 cgaagtccga cgtcccgcac gcgcggaagg tgatggaggc ggtggtcgag gaggtcggtg
     9661 cggcacgaca cacgaccgat gaggagatcg ccaagatctc gctcgtcggc gccggcatga
     9721 agacccatcc gggggtggcc gccgacatgt tcagcgcgct ggccgatgcg ggtgtgaaca
     9781 tcgagatgat ctcgacctcg gcgatccgcg tctcgtgcgt gatccgagcg gaggacgtgc
     9841 atcgggccgt cgaagccctc catgcgaggt tcgacctggc cgaggactcg gtcgcgaggg
     9901 agcactgagg atggccgctc ctcgcgtcgc ggtcgcgggc gccacggggg cggtgggtcg
     9961 cgagatcctg cgcatcctcg aggagcggga gacgccggtc gggacgctcg tgccgctcgc
    10021 atccgctcgg tcggcgggca cgcgcctccg gttccgcggc caggaggtcg aggtccggcc
    10081 gctcgacgcg caggactggt cggaaatcga cctggccctc ttctcggcgg gagcgaccgt
    10141 atcgagggag gtggcgccgc gggccgtcga ggccggaacg atcgtcgtcg acaactcctc
    10201 cgcctggcgc atggaccccg gcgtgccgct cgtcgttccc gagatcaacg cccacgcgct
    10261 cgagggccat cgggggatcg tcgcgaaccc caactgcacc gcgatcgtcg cgctcatggc
    10321 gctcgcgccg ctccatcggg ccgcaggcct gagctcggtg atcatctcgt cctaccagtc
    10381 cgtctcgggc gccggacacc gcggtgtgac ggagctcctc gagcaggtcg agaagctccg
    10441 cggccaggag gaggatctga cgcatccgga cctcgacgcg ctgccccgcg gggagatctt
    10501 cggcccgacg atcgcctaca acgtcgtgcc ccgcgcgggt tccttcgagg acgacggctt
    10561 cacgagcgag gagcggaagc tcgccgacga gagccggaag atcctcgagc tcccgggcct
    10621 gccggtcgtc gggacggtcg tgcgcgtccc cgtgatcgcc ggccacgccg tgtccatcca
    10681 cgcgatcttc gagcgcgacc tttcaccggc ggcggcgtgg gagacgcttc acgccgctga
    10741 gggggtgagg gtcgtcgacg accccgcttc ggatctcttc ccgacgccgc tcgagtccgc
    10801 cggcaccgac gacgtgctcg tcggccggat ccgccgcgcg gcagagcgct cgctcctgct
    10861 gttcgcggcc ggcgacaacc tccgcaaggg cgccgccctc aatgcggtgc agatcgccga
    10921 gcggctcctc ggctaggtcg gctcgcagtc gtacaccgtc tcgccgttac ggcagacgtc
    10981 gaccccgaac ccaccgttga gactgtccca gtgccgtccg ccatcgagct cgtcgttgcc
    11041 gtcgtctccc tggagcacgt cctcgcctgc gtttccctcg aggaggtcgt tctgcgcccc
    11101 accgaggagg tcgtcctccc catcgcctcc gaaggcctcg tcctcaccgt cgccccccaa
    11161 cagtgagtca tccccgccgc cgccgtgaag cacgtcgtct ccggcttcgc cgcgcagctg
    11221 gtcgtgccct ccgtggccga acagtcggtc ctctccggtg ccccccagaa gctcgtcgct
    11281 ctccggaccg ccgatgagcc ggtcgtcttc ggacccgccg acgagctggt cggcgcctcc
    11341 ttcgccctct atcgtgtccg ctccgccgta gccgaagatc tgatcggctc cgtctcctcc
    11401 gaacaagcgg tcttctccac cgggctcgcc cacgtggtcg ccggtcatga tgtcgacgcc
    11461 gcttcctccg tgaagcgtgt cgttcccggg gccgccttgc aggcggtctc cagagcctcc
    11521 gccgtacagc tgatcatcgt ccgcatcgcc gatcacccag tcgcggctgc cgccgccgta
    11581 cgctgtgtcc cttccgagac cgccgaggat cgtgtccggg tcggaccccc cctcgagccg
    11641 gtcgtcaccc ggacccccgt cgatgctgtc gctaccgctt ccgccgacga tgcggtcgtc
    11701 tccaccttca ccgctcacga tgtcgttccc gccgccgggc gacagcacgt tcggcacgtc
    11761 gccggcctgc gcggtgaggt gatcggagaa ccggctgccg atgatccctt cgatgtccgt
    11821 tcggacgttg tccgagtccg gcccgtcatc cgccatgttg tcgatcgtga cgacgacccc
    11881 gacactggag ccggcgtagg aaacggtgtc gtgaccggcg ccgccgctga actcgtccgc
    11941 tccgggcccg ttgtagaagc gatcgttccc ggcgtctccc acgacccagt cgttccccga
    12001 cccgtcgcag atgcggtcgt tgccaccgcg gccgtcgatg acgtcggcgc caccgcgcgc
    12061 cacgatcacg tcgttgcggc tcgtgccgtt gatcgtgtca tggccgttcg tgccgacgat
    12121 ggtcgcgcgc ttacctccgc acgtgggggt tgccgccacc gctgccggag cccacacgag
    12181 gagaaggagc atcacggaag cggtcacgag gtcgtgtcgg ttggcgcgca tcgttcctcc
    12241 tcctgactcc cgtgtgggcc gacgcatcta gacggttcgc agcccacgag gacctcgagc
    12301 tgcgtcggtc ggggtcaggt ccactggccc tcgcagccgt tgagcctctc tccgttgcgg
    12361 caaaggtcga agccgccgtc tccgtagagc tcgtcccagc gcgacccgcc gtcgagatgg
    12421 tcgtccgcgt cccctccgta gaggacgtcc tcgcccccct cgccacgcat cacgtcgttg
    12481 tcctcgtggc cgtagaggtc gtcgtcgccg gctccgccga acagccggtc cgctccagcg
    12541 tggccgtaga ggtcgtcgtc tccgtcagct cccgtgagcg tgtccgcccc ggccccgccg
    12601 tacacgacgt cgtcgtggtc tcctccgctc agggtatcgt cgtcggcacc gccgtcgagg
    12661 aagtccccct cgcttccgcc ggacatcgcg tccttgcctt cgttgcccga catcacgtcc
    12721 cacccgcgtc cgccggtcag ctcgtcctcg ccggcgcgcc ccaacatgtg atccgatcca
    12781 tcgccgccac ggagaacgtc atcacctccg tgaccctcga ggtaatcgct gcctccgccg
    12841 ccgaccagca ggtcatcgtc ttcgaagccg ctcaggctgt cccggccgcc ctcgccgtac
    12901 atccggtcct tgccctcgaa gccatccagc tggtcggggt cggggccgcc gtagccgacg
    12961 tcgtcaccag cgccggcgta gaaggaatcc gttccggcac ctccgaagaa gcggtcgttg
    13021 ccgctccctc cgtcagcggc gtcgccgccg gctccgccat cgaagcgatc gttgcccggg
    13081 ccgccctcga gccagtcgtg cccgggaccc ccggtcaacc ggttggccac caggtcagcc
    13141 gaaggcgcgt acagcgagtc gttgcccttg cctccgcgca gatcctccac gtcggttcgc
    13201 acgtcgtcgc tctctccggc gtcgttggcg atgttgtcga tcctgacgac gacgccggtg
    13261 gtccggtcct ggtaggtcac cgtgtcggtc ccggttccgc catggaacac atcggcgcct
    13321 gggccgccgt gcagcaaatc gctcccgcta cctccacgga ccgtgtccga gccggctccg
    13381 ccgcagatcc ggtcgttgcc ccccttgccg tcgatcgagt cgctcccgtc cttcgccacg
    13441 atgacgtcgt tgccggaggt cccgttgatc gtgttgttgc cgttcgatcc cacgatcgtc
    13501 gcggccttgc cgaggcaggt gggcgtggcg gcctgagcag gcggggacac cagcaggagg
    13561 gtgagcgcga gcacggcgag gaacgtgcgg cggacgctcc ccgagagttc cgcggccgat
    13621 gatctcgccg gaccggatgg aggcgtcagg tccatgccat ctcgcaagca tcctcgtgca
    13681 gctccccgtt gcggcattgg tcgtcgcctt cgccggcgtt cagcacgtcg tagtgtcggc
    13741 cgccgtcgag ctggtcgtcg cccgcccccg ccaggagcct gtcatcgccg ttcgtcccga
    13801 acagggtgtc gttctggggt ccgccctcca ggtcgtcgtc accttcgcca ccgtcgagaa
    13861 cgtcgtggcc gatctctccg tagaggaggt cgtcatggcg gccaccgagc agggtgtcct
    13921 cgccgctccc gccctccagg cggtcgtcgt ggtccccacc gtgcaagatg tcgttgtcgg
    13981 ccccgccggt gaggtagtcg ccttccgtgc cgccgtagat ggtgtccttc ccggcctctc
    14041 cgtggatgac gtcccagccg gctccgccgt agagctggtc ctcgccatcg gatccgtgga
    14101 tcgtgtcggc cccggttccg cctcggatat cgtcgtcacc ctcgccgccg gtcagggagt
    14161 cgcgccccgc acccccctcc aggcggtcga cgccatcctc accggtgatg gtgtcgcggc
    14221 cgccctcacc gaagacgcgg tcgttcccct cctggccgtt gaggtcgtcc gggttggctc
    14281 cccccaagat cgtgtcgtcc cctaacccac ccctgatcga gtccgagcca tcgccaccct
    14341 cgaggagatc gttcccgccg agcccataga ggatgtcatt gccggggccg ccgatgaggc
    14401 gattcgcgac cgcttccagc tggggcacca ggtaatcgtt gccgttcccg ccgcggatgt
    14461 tctcgatgtc cgacagcacg ttgtctccct cgtcggtgtc gttgctgttg tcgtcgaggg
    14521 tgacacccac gtctgtgacg tagccctggt aggtcgcggt gtccttgccc tttccgccag
    14581 agaagcggtc gccgccgggc ccgccgtgga gggtgtcgtt gtcgccgccg ccgaacatgt
    14641 cgtcgttccc gccgcccccg cagatgacgt cgttcccgct ccggccgttg atgacgtcgt
    14701 tccccccgcg gccgacgatc acgtcgtccc cggcggtgcc gttgatggtg ttgtcaccgt
    14761 tggtgccgac gatcgtggcc ttcttgccca ggcaggtggg tgtggccgcc tgcgccggag
    14821 agaggccgac cagcaggatc ccgctgatca aaccggtcag ggtgatacgt gcacgccgtt
    14881 gcatcgatcc ctccctcaga cggtctcgca gccgatgagg atctcaccgc gcgtgcatgc
    14941 atcgagcgtc ccgtcgccac cgtccagccg gtcgaagtgg cgtccgccgt ccagcgcgtc
    15001 gtcgccggcg ccgccctgga tcgagtcgtc tccatcccca ccgaagagcc gatcgttgtg
    15061 ctcatcgccg cgcaggtcgt cgcctccggc gttccccctc agctcgtcgg tcccggtccc
    15121 gccgatcagg tcgtcgtcac ccgagccgcc caggatttcg tcgttgccgt ccccgccggt
    15181 gatgctgtcg tatccggacc cgccctccag gaagtcgttt gcggctccgc cgaggatgat
    15241 gtcgccgcct ccgcctccaa ggatggagtc gctccccgcc tcaccgagga gctggtcccc
    15301 ttccgtcccc ccgtagatga tgtcggaccc atcgccgccc tggatcgagt cctccccttc
    15361 tccgccccag acctcgtcgt cgccgccgcc cgcgagcacc cagtcgcggc ccggacccgc
    15421 gtccacgaaa tccgggaacg gggcgaccgc gtcggcgatg atggcgtcgt tgcccgcgtc
    15481 tccgaacagg gtgtcttcgc cgtcgccgcc gctgaggctg tcgcgggcgt cgcccccgaa
    15541 gatgacgtcg tcatcccctc cgccccgtag gtcatcgcgc ccgccttcgc cgaagatcca
    15601 gtccacgccg gcgccacctt cgatctcgtc cggatcgggg ccaccgaaga ggtcgtcatg
    15661 gccctcaccg ccctcgaggt agtcggatcc gcttccaccg tcgagcaggt cggcttcgcc
    15721 gaagcccgcg atcgtgtcgt cgccaccgcc gcccgagagg acgttggagg acgcattgcc
    15781 gctgatcagg tcgtcgtatc ggcttccgac cacgccttcg acatcggttc ggacgtcgtc
    15841 tccctccccg ccgtcgttcg cgacgtcgtt gatcgaaacg atcaccaggg aggcggagcg
    15901 tgcgtacgag accgtgtcgc gtccgccgcc tccgctgaac acgtcgcttc cggcgtcgtt
    15961 gtagaagcgg tcgttgcccg catcgccgtt cagcgtgtcg ttgccgtctc cgccgcagat
    16021 gcggtcgttg ccgcccttcc cgttgatgac gtcgttcccc ccgcggccgt ggatcacgtc
    16081 gtcgccgctg gtgccgttga tggtctgacc ggcgctcgtg ccgacgatcg tcgcggtctt
    16141 cccgccacag gtcggcgtcg cggcgtgcgc gtgggaggct gcgagggtga cgagggctgc
    16201 ggccaagatg gcaggagcca gggtacggcg acgtgatgcg cgcaagatgg gacctccctc
    16261 ccggaaaacg gacgaacgag tctttctagc cgttcaacag gatactgtca agggtcagag
    16321 agggcacgcg cacggcatcc cgccgcactt cgggcatccg tgctcgtacc gggcagccgc
    16381 atctgcgatg tccacgccca ggaggttggc cagcgaggtg agccacgcca gggcgtctcc
    16441 gacctcctcg cgcaggttct cgggatcccc ccgccgcatc gcgcgcgcga cctcgccgac
    16501 ctcctcgacg agccagcgga acgtcgcgtc gggaccacgc gccgcgtccc gctcggcgta
    16561 ggtgcggcgc atcagctcct ggaagtcccg gagatccatc agcgttcgcg tcggctcgtg
    16621 agccaggcgc ccgccagcac gaggcccatg ccgacgagct ggagggggtc cacctgctcg
    16681 tccagcagca tgacgccgag gatcgtggcc acgaccggga ggaagtagat cgcgaccgcg
    16741 ccgcgcgtgg cgcccgcccg gccgacgagc gtcgccatcg cgaggaacgc catccctgag
    16801 ccgaacacgc cgaggaccgc cgtcgaggcg aggctcgacg cgtcgagggt cgagccccgc
    16861 agggacagga gcccgaccgg caggagcagc acgaccgcgc tgagctgcgc gcgcaggacg
    16921 atcggaagcg cgccgtaccg ctgctggagc ggcaccgtga ggttcgcgga gagcccgtag
    16981 agcatcgtcg cgaacaggac gagcacggcc cccaggagcg ccgcgctgcc ttcgcgcgtc
    17041 ccgggcgagg tgaggagcac cacgccggcg aagccgacga gcagaccggc gacctgcacg
    17101 gggcgtggca tccgccgcag caggatcgcg gcgaacagtc cggcgaacag gggcatcccg
    17161 ccgttgatca tcccggcgag cgacgaggtg atccactgct gggcgaccgg gaacagcgtc
    17221 agcgggatcg ccatccagat cgccccgagc agcgcgatcc gtggccagtc ctcccgcgcc
    17281 accgggcgcc gggccctggg gatgaccgcg accgacgccg ctccgagcgc gatccgcagg
    17341 aacgcaacca ggcccggctg aagaccctgg agcccccact cgatgaagac gaacgacgag
    17401 ccccacatca gcgcgatcgc gctcagcagg ccccactcga ccggcccgaa cgcctcacgt
    17461 cgcgttcccg gggcggtcga gatgacggga gcggtccgca cgcgccccag cgtacccgcc
    17521 gtggtatgga tgggaccatg cgccgtctcg agagcgtgat cctctacgtg gaggacctcg
    17581 atcgttcgat ccgcttctac ggggacgtcc tcggtctcga tctcgcgctc agcgagcacg
    17641 gctacgccga gttccggctc gagaacgtga agctcgccct gtacgagcgc gagcgggcgg
    17701 tgaggctcac gggagccgag cgtccccccg ccggcgcggc ggagatcctg ttcgtggtgg
    17761 aggatgccga tcggtgggcg gagcggctcg ccgaccacgt cctcgccggg ccggtcgacc
    17821 acccctgggg tcaccgcagc gtccacgtcg cggatccgga cgggcacgtg gtggagctcg
    17881 cccaggagat cccgcggacg cgtccgcgcg gggcatgaag gaggggcgag gcgcgtgcct
    17941 cgcccccggt ggtcgggacg gcgggatttg aacccgcgac ctcagcgtcc cgaacgctgc
    18001 gcgctaccaa gctgcgctac gtcccgacgg ccgaacggcc ggacctggat ggtacaccag
    18061 ggccgcgggc cggctcagcc ccgaccggtg tagcgcagga tgtcgacgcc tcggttgtag
    18121 tccaccgagt agaggatgtc gtcaccggcc cagtaggccg cggacgtcga cccgccgtac
    18181 ggcacgtagt agccgatctc ccgcacccgt ccttccttcg agacgcgcaa gagccgtgtc
    18241 ccgtgctcgt accacgccat cgcgacgacg cccccgttcc ggtacgtcgg gtgggtgtcg
    18301 aaccagtgcg cgcagaacag gtcggccggc gcgtcgccgt cggtgaacag accgttgcgc
    18361 accgtgtacg acccggtctg ggcgatcgtt ccgtcctcgg cccagcgcga gacgtcgaag
    18421 gtccgcaaga cggcgctccc gttcgccgag cagttggccg tggggcccgt cgactcaccg
    18481 cccaccagga ggaagcggtc ggtcccgccg ttcggccaca ggttcccgtg gttgaaacgg
    18541 ctctcgctcg gcccacgggc cacgagccgg gggttcgccg ggttctcccg agcgtcgagc
    18601 agcaggaacg ggttgctcga ggtgacgacg aggccgggcg agatctccgt cacgtcgtgg
    18661 aggttgttcg tcgcaggctt cccggtcctc cagttgccgg cgaggaccgt gtggttcgcg
    18721 aggttgacga tctgtccctc ggagccgtag gcccacgtgc agttcaggac gcacgaccac
    18781 gtgtgggggt tcgccggcag gttgccctcg cggagcgtgg ggttcttcgg atccgagacg
    18841 tcgacgacct tgcgcccgaa gagcaggatc ttcccgttcg tgtccacgtc ctcttcgccg
    18901 aggtaggggg cttcctccgc cgggatcgcg aggagaccga cgagctgcgg gtcggcgggc
    18961 tcgctgatgt cgtagatctt caggtgctgg ctggtcgtca ggtacatgag gttcccgacg
    19021 atgcgcgcgc cgacggcatc gttgttcgtc gcgaggtgcg cgacgaactc gaggttcggc
    19081 gagcgccagc cgccggcgtc cggcagggcg cgccggcccg ctttcggcat cgcgtaggcg
    19141 agcgtgccga acgtccggtg gacggtcgtc gagcccaccg agtcgcgtcg gccgagcgtg
    19201 cccgcgacgt agagacgctg cgatgcgctg tcgatggcga tcgcggccgc gcgatcctcg
    19261 tcggtcaggc gcacggcgtc gtccgtgcgc atcccgtagc gagcggacca gacctcgtgc
    19321 cccgatccgg cgtcgtagcc gagcgcgacg taacgagatt gcgccggaag gaacgtcccg
    19381 ctccacccgg cgatgtacac gcggtcggtg tcggggtcgt agtcgatggc ggacccaccc
    19441 tcgtcgagat gaccggggaa ggcgtacctg gcgtcccagg cgagcgcgcc gtcggtcgct
    19501 tcgaaggcca cggtggcgat gtcgaagcgc tcggcggggc ccgcctcgga gcggcccgtc
    19561 acgaagaccc ggtcgccgcg ctcgtcgacg acgaggtcgg cgccgaagct ctgccctgtc
    19621 gaggccggcg acgcgtagct gctgaaccac cgctctgcac ccgtcgcggc gtcgagggcc
    19681 tggagcgcgt accgggggct gctcgtcgcc gacccgacca cggcggtacc gcccacgaac
    19741 agcgttcggg cgtcggggct gagggccagg gccgcccccg cgtcgaaggc gttgccgccg
    19801 ccgtcccgac ggttcgtcca gcggacctcg ccggtcgcgg cgtcgatcgc gagggtcacg
    19861 at
//
LOCUS       DATLGG010000156         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_42476, whole genome shotgun sequence.
ACCESSION   DATLGG010000156 DATLGG010000000
VERSION     DATLGG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42476"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..776
                     /locus_tag="VM638_04495"
     CDS             72..776
                     /locus_tag="VM638_04495"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVM11715.1"
                     /translation="MRTHDDLRDLLGAYALDAVEPDERRALDQHLRTCADCRREITEH
                     QEALAGLAEPVAPPPQVWERIETAAREDDAATVVPLRPRRPRLVAALSAAAVVAFAFL
                     GWRVAVLDARLERVAGDTLVASATAALADPEAERVVLRSGRGTLAVDAVVLPDGRGYV
                     LQGNLDALPEDQTYQLWAMVGEQPISVGVLGTEPSVTAFRVGSGTSALAITVERAGGA
                     PQPTSDPIAHAPLSVA"
     gene            933..1247
                     /locus_tag="VM638_04500"
     CDS             933..1247
                     /locus_tag="VM638_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVM11716.1"
                     /translation="MGSTEEATVTVTNFRYEPPTLEVPTGTTVTWVNQDEILHTVTSG
                     TPEGATVPGVSEGAAAEPDGRFDEELDGGGATVEVTLDERGTYTYFCAIHGGMVGEII
                     VG"
     gene            1313..2035
                     /locus_tag="VM638_04505"
     CDS             1313..2035
                     /locus_tag="VM638_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11717.1"
                     /translation="MERTERKVWRGGMVAALLAVAVIGTACGTGGAQERSTDSQPSAS
                     AHGAPSTGSGIDTPGAELQQTLTSLLTSHVFLAGIAVEQAALTKDPESAQFKAAAEAL
                     DKNSQDLAGAIESVYGPEAGDQFLDLWRAHIGMFVDYTVGGLTGDDKMQDKAKSELDQ
                     YRQDFGAFVDSATGGNLPAEDVAAALQEHVDSLVVTIDQVIEGKGNAFMSLYEAAQGH
                     MPATASALAGGIIAANPDKFAA"
     gene            2210..>2418
                     /locus_tag="VM638_04510"
     CDS             2210..>2418
                     /locus_tag="VM638_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11718.1"
                     /translation="MGARRSNHPLRTLLPRAATTFSLLLAIGLFAPAGAGDDEHVKDA
                     KTPLLHEADTLQPVVEEPLGEMRCE"
BASE COUNT          395 a          821 c          884 g          318 t
ORIGIN      
        1 atcaagagcc ggatccgctc cgggctgcgc cggatgcgag gcgccatgag cgacgagatg
       61 gtggacgaac gatgaggacc cacgacgatc tccgggacct cctgggggcg tacgccctcg
      121 acgccgtgga gcccgacgag cggcgcgcgc tggatcagca cctgcggacg tgcgccgact
      181 gccgtcggga gatcaccgaa catcaggagg ccctcgcggg cctggcggag ccggtggcgc
      241 cgccgccgca ggtctgggag cggatcgaga cagcggcgcg cgaggacgac gcggcgacgg
      301 tggtaccgct gcgccctcgg cggccccgcc tcgtcgccgc tctctccgcc gcggcggtcg
      361 tcgccttcgc gttcctggga tggcgggtgg cggtcctcga cgcacggctg gaacgggtgg
      421 ctggggacac cctcgtggcg tcggcaacgg cagccctcgc ggatcccgag gccgagcggg
      481 tcgtcctgcg ttcgggccgg ggaaccctcg ccgtggacgc cgtggtcctc cccgacggca
      541 ggggatacgt cctgcagggg aacctggacg cgcttccgga agaccagacg tatcagctct
      601 gggcgatggt cggggagcag ccgatctccg tgggcgtcct cgggacggag ccgagcgtca
      661 ccgccttccg agttgggagc gggacctcgg ccctggcgat cacggtggag cgcgccggtg
      721 gcgcgcccca gccgacatcg gacccgatcg cgcacgctcc cctctccgtg gcctgacgcg
      781 tcatccgcgt ccccgcgcgg ctccgaagat gggtcatgaa gaacctgcga acgttgatgc
      841 ttctggtctc cctggggctc gccgctcagg cctgctcctc agcgccggtc acggaaacgc
      901 gtacagggaa cggatcgacc ccccaggggc cgatggggag cacggaggag gcgacggtca
      961 ccgtcacgaa cttccggtac gagcccccca cgctcgaagt tccgaccggc accaccgtca
     1021 cctgggtcaa ccaggacgag atcctgcaca cggtcacgtc cggcaccccc gagggggcga
     1081 cggtccccgg cgtatctgag ggcgccgcgg ccgagccgga cggccggttc gatgaagagc
     1141 tcgacggcgg cggagcgacc gtcgaggtca cgctggacga gcgtggcacg tacacgtact
     1201 tctgtgcgat ccacggcggc atggtcggcg agatcatcgt cggctgagtc atccgtccac
     1261 acgcacggct ccgaatcccc tgtgacaccg acccccgaag gagggatcga ggatggaacg
     1321 gacggaacgg aaggtatggc gcggcgggat ggtcgcggcg ctcctggcgg tcgccgtgat
     1381 cggcacggcg tgcggcacgg gcggggcaca ggaacggtcg acggacagcc agccctcggc
     1441 cagtgcgcat ggtgcgccga gcacggggag cggcatcgac acgcccggag cggagctcca
     1501 gcagacgctc acgagcctcc tcaccagcca cgtgttcctc gcgggcatcg cggtcgagca
     1561 ggcggctctc acgaaggacc ccgagtcggc gcagttcaag gcggcggcgg aggcgctgga
     1621 caagaactcc caggacctcg ccggcgcgat cgagtccgtg tacgggccgg aggcgggcga
     1681 ccagttcctc gacctgtggc gcgcgcacat cgggatgttc gtcgactaca cggttggcgg
     1741 gctgaccggt gacgacaaga tgcaggacaa ggcgaagtcc gagctcgacc agtaccgcca
     1801 ggacttcggc gccttcgtcg actccgcgac cggcgggaac ctcccggcgg aggacgtcgc
     1861 ggcggccctg caggagcacg tggacagcct cgtggtcacg atcgaccagg tgatcgaggg
     1921 caagggcaac gccttcatga gcctgtacga ggcggcacag ggccacatgc cggccacggc
     1981 atcggccctc gccggcggga tcatcgcagc gaaccccgac aagttcgccg cctgatcacg
     2041 atcggaacgc agctcgggaa cgggcgtccc catcgggggg cgcccgttcc gcgttcggca
     2101 gcgcgatgga agacaggacg ggctaccagc agggtggctg cttgcctcgg tgtggcttcg
     2161 acgcgctccg gaaaggctag gaggagccga ccatacgagg tgatgcatca tgggtgcacg
     2221 taggtcgaac catccgctcc gaacgctcct ccctcgggcc gcgacgacgt tctcgctgct
     2281 actcgcgatc gggctgttcg ccccggccgg agccggggac gacgagcacg tgaaggacgc
     2341 gaagacgccg ctgctccacg aggcggacac cctccagccc gtcgtcgagg aaccgctcgg
     2401 tgagatgcgg tgcgagga
//
LOCUS       DATLGG010000157        12027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_69476, whole genome shotgun sequence.
ACCESSION   DATLGG010000157 DATLGG010000000
VERSION     DATLGG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69476"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VM638_04515"
     CDS             complement(<1..1008)
                     /locus_tag="VM638_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVM11719.1"
                     /translation="MKANRILTCASLVAVVGALTFAAPATASHAKPDLDQIDPVRFGL
                     QDAIVERKIITARDGVTSLALDIIRPKTEERVPTIFFQSPYYNTLGRGFRAERKTPWT
                     SMAALNPPTPWAPFPEWYDEYFVPRGYAIVMQDQRGTRNSSGCQVYGGREEITDAVDT
                     IEWIERQPWSNGAVGMTGGSYDGTIATGAASMAPRALKAIIPIRAIDRWYDYHHFNGL
                     PSSQHASTAEGFTVTRPLTDLQNSVNEDTLWAAHVAERRGCAASVGAVVAAAYKPGYQ
                     DSRSSFWSERDYLRDADRITAATFIIHGLNDTNVKTTNAGHLWDALPASTPKKLWWLR
                     GG"
     gene            complement(1090..1608)
                     /locus_tag="VM638_04520"
     CDS             complement(1090..1608)
                     /locus_tag="VM638_04520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVM11720.1"
                     /translation="MHNVRLDRIEETKGRAASDPQATMLQVNMAGAWQADDGDPQFAG
                     TVTFAQGDARLEADFPPFLGGDGRTPSPLTYCFFGAMACYGATFAMQAAMAGVEIRDL
                     RVTLALSVDFHTALGLGEHVPLSRFDFEVQVDTDASDEEIQRVKQLADERCPAIWAME
                     NPVPYRTRAQKV"
     gene            1652..2848
                     /locus_tag="VM638_04525"
     CDS             1652..2848
                     /locus_tag="VM638_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVM11721.1"
                     /translation="MAEQSGTTSRRIQELADLLVPVVRFFDRSARARERAGEAACDFR
                     AGDPQELAPSWLVQALQQAAIPRDAGWYAYTTELPAAQEAVAASLRERFGIAFRPEDV
                     ALTNAAVAAIAICLQAIIDPGDEVIITTPPHFLYEPLIRMPGGVAVRVPADPETFELD
                     LDATAAAITGRTRAVLVNSPHNPTGRIYRPETLRNLAALLTGASERNGRPIYLLSDEA
                     YQRVLFGEVAFETPTAFYPRSFLIYTYGKTLLAPGQRLGYIAMPPTMPNRADLRRALS
                     AAQLVLGWAYPTALLQHALPELERHTIDLQALRRKRDRMVEALRAFGYELHVPEATFY
                     LLVRSPDPDDQAFVDAMAEDGVLLMPGGLLEAPGFFRISLTATDDMIERSLPVFERAI
                     KERSGG"
     gene            2841..3014
                     /locus_tag="VM638_04530"
     CDS             2841..3014
                     /locus_tag="VM638_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11722.1"
                     /translation="MARRRRLWPRVERAILGAGMGVAAFLIERRLRKVLDEMRSGGDG
                     PAATLGPADGSRR"
     gene            complement(3022..5064)
                     /locus_tag="VM638_04535"
     CDS             complement(3022..5064)
                     /locus_tag="VM638_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HVM11723.1"
                     /translation="MKSILAATDRSASGDRAVRHAADLAARYEADLVVLQVLLPADGS
                     EGATAAQEAARVTHANEELARFAEELAGPRGRPRVVVDLDPAAAIVETARREGVDAIV
                     VGNVGMAGRKKFLLGNVPNRVSHNAPCSVFIVDTSDPSAPKPEPVITRSEPEVEGRLL
                     GRATRIGRVMSRNGLRDLLKPAGPGDDLRERARRLRTAFEELGPTFGKLGQILSTRPD
                     LLPPEFIDELSTLQSQVPPLTEEQVVSVMEEELGVPWEDVFESIHPEPLAAGTIAQVH
                     WATLADGEEVVVKVQRPTARQDIMQDLGLLQMFAEKTRGKAAFKQLVDLEAIVEHLST
                     SLQQELDFRQEAANGERLRKVLEPYPRLDVPGVHTDFSTDRLLVLQAIPGVKITDSED
                     TPERRDAARQLLESYYRQILTDGFFHADPHPGNLLWHDGKIWFLDTGMVGEVGPEIRE
                     YLLLVLMAFWQHDVSFLSDVVLMLTGEDQRPDLDVTRFEADLGRMVDRSRDTSLREIQ
                     LGPILQEITEISVKHNVPLPASLALTGKALAQMQLVVAQLDPELDPFQVAGTFVVKGL
                     TERLRSKADPRRLFYEGQKLKLRVVRLVESFERLTGARPGPKLQVHFRGTESLEGTVR
                     RAARRIAVSMAAGSAIIGAAVLGASPTVENWVPASLGTLGGILTGGLLWDLLRAGK"
     gene            5166..5789
                     /locus_tag="VM638_04540"
     CDS             5166..5789
                     /locus_tag="VM638_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVM11724.1"
                     /translation="MDHPTAVTERGEETRRRILDVAGRSFAEYGYQGTSFSHLVAATG
                     LTKGAFYFHFASKEALALAAFAHAQERWVTAVVRRLPIDAPAADRLRSLLRAAVRFYK
                     EDPAGRCVFRLCLELGEDPRLRPTLAPHLALWEDLVESLVRLAQEQGDYRADLDPRVV
                     ARVAVAAFVGVTDVARAMTGGQDLARRAEEFLHVFESGLRRTGRERA"
     gene            5786..8092
                     /locus_tag="VM638_04545"
     CDS             5786..8092
                     /locus_tag="VM638_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVM11725.1"
                     /translation="MKGRPATFYRLGAFAHRRRWWVLTAWVLLFVAALPPLGKLTERL
                     SQGGFEVPGSGSARALELAEEFQGHHEIVTTLVMWSDTRLAGDPAFRALFEEVRAALV
                     VGPGVAEVSDPYRTPERSISPDGRVLTAAVGLSDDQDAALRHADELNRIVAEASAGAR
                     DVRALLTGDAPFYAAFTETTTHDLERAERIALPISLLILILAFGSLVAAGLPLILALL
                     GLAVTFGVISLVAATTTVSIFTQNVASMIGIGVGIDYSLFLLTRFREELKAGRPTGDA
                     VAAATATSGKAVFVSAMTVVVSLSGILLVDIAAMRSMGLGAMIAVAVAAAAALTLLPA
                     LLSMLGPRVDRWAIRRRTTARPGRGGWWHRWARTVMRRPWRALAATALVVGVLAWPAL
                     HLRLGSSGPDIFPADSGPRVAAEVTAAAFGEGVVSPVQVFVELPRDVLGEGLEDIGRV
                     SAALAADPEVASVISVASVIPPDLPPERVAGFLASPAGAEATAGMMSRDGRLTAISVI
                     TKHGPQHPENQALVERLREDLPGLLPPGAEVAVGGSPALNLDINREMERSILPVVGMV
                     LTLSFLLLLLFFRSLLLPLKAILMNGASVLAAYGLLVFVFQDGHFQELLGFDTAGHIE
                     MFIPLFLFSILFGLSMDYEVFLLARIREEYLRTGDNTEAVGWGLEHTGRIITSAAAIM
                     VTVFGAFAFASLVPIKAMGFGLAVAVFIDATLVRIVMVPATMRLMGRWNWWLPRWLDR
                     ILPNVSLEGEVAPVAGQAAAEDPVPAKA"
     gene            complement(8143..8676)
                     /locus_tag="VM638_04550"
     CDS             complement(8143..8676)
                     /locus_tag="VM638_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11726.1"
                     /translation="MPRSIALALFAALTLTLSACERATPETAPAPAPSPIQQINPEDL
                     PRCDYPPEVRDPRWLPKNLPLPDGSYTMERLEPLAGYKRVLLVIPITLDELTTFVLEE
                     WPKQGWVLGRGDAEPGEIEHQFSKAPAVGAFKAQAVYCDPGYSVMYLIYAPDRPPDPD
                     AAPGIPSPRGSPLNPAA"
     gene            8705..9628
                     /locus_tag="VM638_04555"
     CDS             8705..9628
                     /locus_tag="VM638_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11727.1"
                     /translation="MPLVIQVVVTGVAAGAVYGLVATGYALVYRLTGVVHLAFGELLG
                     LAGFAMLLAAAGSDPLVAGGISPGRYAIGLGAGLAVAVLSSLVLYAIVRPFLRPGREL
                     GWIGAVAAVAFAVRGVLASSFARESYVFPDLLPLHRLGDAGVLRLGGGVTLPIRTFFV
                     IAVGFALAAAMARLLDRSRVGVAFRAIAEDPMAARAVGLPVDALRFAAFALAGGVAIL
                     AVIVSAPSAPFGAATGGAIGLKGLVAALLAGFGSPWRALGAGAAVGIVETAVSVSPLP
                     LIPGELRDVVPLLLALVLLAGRRRTASLEVS"
     gene            9706..11379
                     /locus_tag="VM638_04560"
     CDS             9706..11379
                     /locus_tag="VM638_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVM11728.1"
                     /translation="MLTATASAAYLALAAVGLNLSVGLAGMPSLGHGAFVGLGAFGAA
                     WARTRLGFDPVAAALAGAVLAAAAGAVTGAGLAKLRPVMLAVSTWILAWLVVTVLAAF
                     PGLSGGARGIPLPEGVLVLEGIDRLIRFTPGVHHGVAVVLLGAALLAYRRTDLGPAGI
                     HLAAARGAPAAADALGLPAERLRGGTFVASAAVAGVAGGFSVQLHGVADASAYGPLLS
                     VELLLAVLIGGAGATLGPVVGTLALVTLPSAARLVAGLLGADASGFEAVITAVLLLGI
                     LAAGGRGLLPPRRGSAARVEPADPPPPPTATLEAHDLARSFGGVRALEGVSLRVATGE
                     VHAVIGPNGSGKTTLLRILAGADRPDAGEVRLDGRGLSQAGVVPRLRAGVARTLQRTM
                     PFGALRTEEHVAAGAAIHRRTGPLRWLLATPAARREDRAVGAHARGVLRSLGLEWLEG
                     RGVRSLDGAERRLMSLATAVGAAPSILLVDEPSAGMAPREAQVVAEMLRRLRDRGLGL
                     LVVEHNVSLVHEVADRVTVLEAGRVIATGTPAEVVRDPRVQEAYLGRTG"
     gene            11393..>12027
                     /locus_tag="VM638_04565"
     CDS             11393..>12027
                     /locus_tag="VM638_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM11729.1"
                     /translation="MRLLPVALALSLASCGGGAPAGLPTELTVVVNAPFSAAPWVADA
                     IERGTRLAAEEINADGGIETAGERYTLAVRTMDTGLSPQRAVENVRTAVADGVLAIVD
                     EGTGVDASWEVARDADLPIGIVFQGGMGLVDHEARPNVFRIAPTDRGIAFRYAEYLVP
                     KGLRIAFLHDDTGYGQEGAAALRRAFECCPEAVEEDLTIPADGDPAPHVLEA"
BASE COUNT         1425 a         4334 c         4389 g         1879 t
ORIGIN      
        1 gccgccgcgg agccaccaga gcttcttcgg cgtcgacgcc ggcagcgcgt cccacaggtg
       61 cccggcgttc gtcgtcttga cgttcgtgtc gttcagcccg tggatgatga aggttgccgc
      121 cgtgatgcgg tccgcgtccc ggaggtagtc acgctccgac cagaagctcg agcgcgagtc
      181 ctggtagccg ggcttgtagg cggcggcgac caccgcgccc acgctcgccg cgcatcctcg
      241 gcgctccgcc acgtgcgccg cccacagcgt gtcctcgttc accgagttct ggaggtcggt
      301 cagcgggcgc gtgaccgtga acccctcggc cgtgctggcg tgctgggacg acggcaggcc
      361 gttgaagtgg tggtagtcgt accaccggtc gatcgcgcgg atcgggatga tcgccttgag
      421 cgcgcgcggc gccatcgagg cggcaccggt cgcgatcgtc ccgtcgtagg agccaccggt
      481 catcccgacg gcgccgttcg accaaggctg acgctcgatc cactcgatcg tgtcgacggc
      541 gtcggtgatc tcctcgcgac cgccgtagac ctggcagccc gaggagttcc gggtcccgcg
      601 ctggtcctgc atcacgatcg cgtacccgcg ggggacgaag tactcgtcgt accactccgg
      661 gaacggcgcc catggtgtcg gcgggttgag cgccgccatc gacgtccacg gcgtcttccg
      721 ttcggcgcgg aagccgcgtc cgagggtgtt gtagtagggg gactggaaga agatcgtcgg
      781 gacccgctcc tccgtcttcg gccggatgat gtccagggcc agggacgtca caccgtcgcg
      841 cgccgtgatg atcttgcgct cgacgatggc gtcctggagc ccgaagcgga cagggtcgat
      901 ctggtcgagg tcgggcttcg cgtgcgaggc cgtcgcagga gccgcgaagg tcagcgcccc
      961 aaccaccgcg acgagggatg cgcaggtcag gatccggttc gctttcatgg cacgaggatt
     1021 cggcacggag gcccgcgtct ccttcgtgca ggtgcacaaa ccccccgcca caccgcgggg
     1081 cgcgcccccc tagaccttct gggcgcgggt tcggtaaggg acggggttct ccatcgccca
     1141 gatcgccggg cagcgctcgt cggcgagctg cttgacgcgc tggatttcct cgtcggaggc
     1201 gtcggtgtcg acctgcacct cgaagtcgaa gcgggagagg gggacgtgct cgcccaggcc
     1261 gagcgccgtg tggaagtcga cgctcagcgc cagcgtgacg cgcaggtcgc ggatctccac
     1321 cccggccatc gccgcctgca tcgcgaaggt cgccccgtag caggccatcg cgccgaagaa
     1381 gcagtaggtg agggggctcg gggtccggcc gtcgccgccg aggaaggggg ggaagtcggc
     1441 ttcgagacgc gcgtccccct gcgcgaaggt caccgtgccg gcgaactgcg ggtcgccgtc
     1501 gtcggcctgc cacgcaccgg ccatgttcac ctgaagcatc gtcgcctgcg ggtcgctcgc
     1561 cgcccgcccc ttcgtctcct cgatccgatc gagccgcacg ttgtgcatcg cgaagcctcc
     1621 tcgctgggtg tgcgcgtagc ataggccccg catggctgag cagagcggca cgacgtcccg
     1681 gcggatccag gagctcgcgg atctgctcgt ccccgtcgtg cgcttcttcg accggtccgc
     1741 gcgcgcgcgg gaacgcgccg gcgaggccgc ctgcgacttc cgggccggcg acccccagga
     1801 gctcgcgccc tcgtggctcg tgcaggcgct ccagcaggcg gccatcccac gtgacgccgg
     1861 gtggtacgcc tacacgacgg agctgcctgc ggcgcaagag gcggtggcgg catccctccg
     1921 tgagcgcttc ggcatcgcgt tccggcccga agacgtcgcg ctgacgaacg cggccgtggc
     1981 ggcgatcgcg atctgcctgc aggcgatcat cgaccccggg gacgaggtca tcatcacgac
     2041 ccccccgcac ttcctctacg agcccctgat ccggatgccc ggcggcgtcg ccgtccgcgt
     2101 ccccgccgat ccggagacct tcgagctcga cctcgacgcg accgccgcgg cgatcaccgg
     2161 gcggacgcgc gccgtcctcg tcaactcgcc gcacaacccg acgggccgga tctatcgacc
     2221 cgagaccctg cggaacctcg ccgcgttgct gacgggggcg tcggagcgga acgggcggcc
     2281 gatctacctg ctgtcggacg aggcgtacca gcgggtgctg ttcggcgagg tggcgttcga
     2341 gacgccgacc gccttctatc cgagatcgtt cctgatctac acgtacggca agaccctgct
     2401 cgctcccggc cagcggctcg gctacatcgc gatgccaccc acgatgccga accgcgccga
     2461 tctccggcgg gcgctctccg cggcgcagct cgtgctcggc tgggcgtacc cgaccgcgct
     2521 cctgcagcac gcgctccccg agctcgagcg ccacacgatc gacctccagg cgctccgtcg
     2581 caagcgcgac cggatggtcg aggcgctccg cgccttcggc tacgagcttc acgtccccga
     2641 ggccaccttc tacctcctcg tccgctcgcc cgatcccgac gaccaggcgt tcgtcgacgc
     2701 gatggcggag gacggcgtcc tgctgatgcc gggtggtctc ctcgaggccc cggggttctt
     2761 ccggatctcg ctcaccgcga ccgacgacat gatcgagcgg tcgctcccgg tcttcgagcg
     2821 cgcgatcaag gagcgctccg gtggctaggc ggcggcgcct atggccgcgc gtcgagcggg
     2881 cgatcctcgg tgcagggatg ggggtggccg cgttcctgat cgagcgccgc ctccgaaagg
     2941 tcctcgacga gatgcgcagc ggcggcgacg gtcccgcggc gaccctcggt ccggcggacg
     3001 gatcccgccg ctagcgcggc ctcacttccc ggcgcggagg agatcccaca gcaggccgcc
     3061 cgtcaggatc ccgccgagcg ttccgagcga ggcggggacc cagttctcga ccgtcgggga
     3121 cgccccgagg acggcggcgc cgatgatcgc cgacccggcc gccatggaca cggcgatccg
     3181 tcgcgccgcg cgtcgcaccg tcccctcgag gctctccgtc ccgcggaagt ggacctgcag
     3241 cttcggcccc ggacgtgccc cggtcagccg ctcgaaggac tccacgaggc gcacgacgcg
     3301 gagcttcagc ttctgccctt cgtagaagag ccgccgcggg tcggccttcg agcggaggcg
     3361 ctcggtgagc cccttcacca cgaacgtccc cgccacctgg aacgggtcga gctcggggtc
     3421 gagctgcgcg acgacgagct gcatctgggc cagagccttc ccggtcaatg cgagtgaggc
     3481 cgggaggggc acgttgtgct tcacggagat ctcggtgatc tcctgcagga tcggaccgag
     3541 ctggatctcg cgcagggacg tgtcccggct gcggtcgacc atccggccga ggtcggcctc
     3601 gaagcgggtc acgtcgaggt ccgggcgctg gtcctcgccg gtgagcatga gcaccacgtc
     3661 ggacaggaac gagacgtcgt gctgccagaa cgccatcagc acgagcagga ggtactcgcg
     3721 gatctcgggc ccgacctccc cgaccatccc ggtgtcgagg aaccagatct tcccgtcgtg
     3781 ccacaggagg ttcccggggt gcgggtcggc gtggaagaac ccgtcggtca ggatctgccg
     3841 gtagtaggac tcgaggagct gcctggcggc gtctcggcgc tcgggcgtgt cctccgagtc
     3901 ggtgatcttc acgccaggga tcgcctggag gacgagcagc cggtcggtcg agaagtccgt
     3961 gtgcaccccg gggacgtcga gccgcgggta gggctcgagg accttgcgca ggcgctcacc
     4021 gttcgccgcc tcctgacgga agtcgagctc ctgctggagc gacgtcgaaa ggtgctcgac
     4081 gatcgcctcg aggtcgacga gctgcttgaa cgccgccttc ccccgcgtct tctccgcgaa
     4141 catctggagc agcccgaggt cctgcatgat gtcctgccgg gccgtcggcc gctgaacctt
     4201 gacgacgacc tcctcgccgt ccgccagcgt cgcccagtgc acctgcgcga tcgtcccggc
     4261 ggcgagcggc tccggatgga tcgactcgaa gacgtcctcc catggaaccc ccagctcctc
     4321 ctccatcacc gagacgacct gctcctcggt cagcggcggg acctgactct ggagggtcga
     4381 gagctcgtcg atgaactcgg gggggaggag atccggacgc gtcgacagga tctggcccag
     4441 cttcccgaac gtgggcccga gctcttcgaa cgccgtccgc aggcgtctcg ctcgctcacg
     4501 caggtcgtcg ccgggcccgg cgggcttcag caggtctcgc aggccgttgc gcgacatgac
     4561 gcgcccgatc cgcgtcgcgc gtcccagcag gcgtccctcc acctccggct cggaccgggt
     4621 gatgacgggt tccggcttcg gagccgaggg gtcggacgta tcgacgatga agaccgagca
     4681 cggcgcgttg tgggagacgc ggttcggcac gttccccagc aggaacttct tccgccccgc
     4741 catgcccacg ttcccgacga cgatcgcgtc gaccccctca cgacgggcgg tctcgacgat
     4801 cgcggccgcc gggtcgaggt ccaccacgac gcgcggcctc ccccgcgggc cggcgagctc
     4861 ctcggcgaag cgcgcgagct cctcgttcgc atgggtcacg cgcgccgctt cctgggccgc
     4921 ggtcgcgccc tcggagccgt cggcgggcag gaggacctgc agcaccacga ggtcggcctc
     4981 gtaccgcgcg gccaggtcgg cggcatggcg gacggcccgg tcgccggagg cggagcggtc
     5041 ggtcgcagcg aggatcgact tcaacggccc gatgatacgc gcggacgctc cgcgagaggg
     5101 tcccgcgatc ctgccgcctg ctccgtcctt gacgaaccga ccggtcggta tgtaccctcc
     5161 ccgccgtgga tcacccgacc gccgtgaccg agcgtgggga ggagacgcga cgccggatcc
     5221 tcgacgtcgc cggccgctcc ttcgccgagt acggctacca gggcacctcc ttcagccacc
     5281 tggtcgccgc gaccggcctg acgaaggggg ccttctactt ccacttcgcc tccaaggagg
     5341 cgctcgcgct cgccgcattc gcccacgcgc aggagcgctg ggtcacggcc gtcgtgagga
     5401 ggctccccat cgacgcgccg gcggcggatc ggctgcggtc gctgctccgc gcggccgtcc
     5461 gcttctacaa ggaggatccg gccggtcgtt gcgtcttccg actctgcctg gagttgggtg
     5521 aggacccccg tctccgcccg acgctcgcgc cgcatctcgc cctgtgggag gacctggtgg
     5581 agagcctggt ccgtctcgct caggagcagg gcgactaccg ggcggatctc gacccgcgtg
     5641 tcgtcgcgcg cgtcgcggtg gccgccttcg tgggggtcac cgacgtggca cgagcgatga
     5701 ccgggggcca ggacctggcg cggcgcgccg aggagttcct gcacgtgttc gagtccggcc
     5761 tgcggcgcac ggggagggag cgcgcgtgaa ggggcggccg gccaccttct accgcctcgg
     5821 ggcgttcgcc caccgccgcc gctggtgggt cctgacggca tgggtgctgc tgttcgtcgc
     5881 ggcgctcccg ccgctcggca agctcaccga gcgtctgtcg cagggtggtt tcgaggtgcc
     5941 gggctccggc tcggcgcggg cgctggagct cgcggaggag ttccagggcc accacgagat
     6001 cgtgaccacc ctcgtgatgt ggtccgacac gcggctggcg ggggacccgg cgttccgggc
     6061 tctcttcgaa gaggttcgcg cggccctggt cgtcggcccc ggcgtggccg aggtctcgga
     6121 tccgtaccgg acccccgagc ggtcgatctc tcccgacggc cgggtcctca cggcggcggt
     6181 cggactgtcc gacgaccagg acgcggcgct ccggcacgcg gacgagctca accgcatcgt
     6241 cgccgaggcg tccgccggcg ctcgcgacgt gcgcgcgctg ctgacgggtg acgccccctt
     6301 ctacgccgcc ttcaccgaga cgacgacgca cgacctcgag cgggccgagc ggatcgccct
     6361 tccgatcagc ctcctgatcc tcatcctggc gttcgggtcg ctggtggcgg cgggactgcc
     6421 gctcatcctc gcgctcctcg gcctggcggt gacgttcggc gtcatctccc tcgtcgccgc
     6481 caccacgacc gtcagcatct tcacccagaa cgtggcctcg atgatcggga tcggggtcgg
     6541 gatcgactac tccctcttcc tgctcacacg tttccgcgag gagctgaagg cggggcgtcc
     6601 gaccggagac gccgttgccg cggcgaccgc cacgagcggg aaggccgtat tcgtctcggc
     6661 gatgacggtc gtcgtgtccc tctcgggcat cctcctcgtc gacatcgccg cgatgcggtc
     6721 gatggggctc ggcgcgatga tcgccgtcgc cgtcgcggcc gccgccgcac tcacgctcct
     6781 gccggcgctc ctgtcgatgc tggggccacg cgtcgaccgc tgggcgatcc gtcgacggac
     6841 gacggcgcgg cccgggcgag gtggctggtg gcaccgctgg gcgaggaccg tgatgcgccg
     6901 gccgtggagg gccctcgccg cgacggcgct cgtcgtgggg gtcctcgcgt ggccggcgct
     6961 gcacctccga ctcgggtcct cgggccccga catcttcccg gccgactcgg gtccgcgggt
     7021 cgcggccgag gtgacggccg cggcgttcgg ggagggcgtc gtctcgccgg tccaggtctt
     7081 cgtcgagctt ccccgggacg tcctcgggga ggggctggag gacatcggcc gcgtgtcggc
     7141 cgcgctcgcc gcggaccccg aggtcgcgtc cgtgatctcg gtcgcatcgg tcatcccgcc
     7201 ggacctcccg cccgagcgcg tcgcggggtt cctcgcctcc cccgcgggtg ccgaggcgac
     7261 ggcgggcatg atgtcccgcg acggccggct gacggccatc tcggtcatca cgaagcacgg
     7321 gccgcagcac cccgagaacc aggcgctggt cgaacgcctg cgcgaggatc tccctggcct
     7381 gctcccgccg ggagcggagg tcgcggtcgg gggatcgccc gcgctcaacc tcgacatcaa
     7441 ccgcgagatg gagcggagca tcctgccggt cgtcggcatg gtcctgacgc tgtcgttcct
     7501 cctgctcctg ctgttcttcc gcagcctgct gctcccgctc aaggcgatcc tgatgaacgg
     7561 ggcatcggtg ctcgcggcct acggactgct cgtcttcgtg ttccaggacg gtcacttcca
     7621 ggagctgctc ggcttcgaca cggccgggca catcgagatg ttcatcccgc tgttcctgtt
     7681 cagcatcctg ttcggactct cgatggacta cgaggtgttc ctcctcgcgc ggatccgcga
     7741 ggagtacctg cgcaccggcg acaacaccga ggccgtcggc tgggggctcg agcacaccgg
     7801 gcggatcatc acgagcgccg ccgcgatcat ggtgacggtc ttcggcgcgt tcgcgttcgc
     7861 gagcctggtc ccgatcaagg cgatgggctt cggactcgcg gtcgccgtct tcatcgacgc
     7921 cacgctcgtg cggatcgtga tggtcccggc gacgatgcgg ctcatgggtc ggtggaactg
     7981 gtggctcccg cggtggctcg accggatcct gccgaacgtc tcgctcgagg gcgaggtcgc
     8041 ccccgtcgcc ggccaagccg ctgccgaaga tccggttccg gcgaaggcct agccgaccga
     8101 gcgcggatcg tcccgtcggc cagctgcacg atccgggtgg gtctaggcgg cggggttgag
     8161 gggtgagccg cgcggcgagg ggatgccggg cgcggcatcg gggtccggcg ggcggtccgg
     8221 cgcgtagatc aggtacatga ccgagtagcc cgggtcgcag tagacggcct gcgccttgaa
     8281 cgcgcccacg gccggcgcct tgctgaactg gtgctcgatc tcgccgggct cggcgtcgcc
     8341 ccggccgagg acccacccct gcttcggcca ctcctcgagg acgaacgtcg tcagctcgtc
     8401 gagcgtgatc gggatcacga gcaggacgcg cttgtagccg gcgaggggct cgaggcgctc
     8461 catcgtgtag ctcccgtccg gcagcggcag gttcttcggc agccagcgcg ggtcgcggac
     8521 ctcgggcggg tagtcgcacc gcgggaggtc ctcggggttg atctgctgga tcggcgacgg
     8581 cgccggggcc ggtgcggttt ccggcgtcgc tcgctcgcag gcggagaggg tgagcgtgag
     8641 ggcggcgaag agtgcgaggg ctatactccg cggcacgtcc caggcccttc caggaggaga
     8701 ccgcgtgccc ctcgtcatcc aggtggtggt caccggcgtg gccgcgggag cggtctacgg
     8761 actggtcgcc acaggatacg cgctggtcta ccgcctcacc ggcgtggtgc acctcgcgtt
     8821 cggcgagctc ctcggcctcg cgggattcgc gatgctcctc gcggctgcgg gaagcgaccc
     8881 gctggtcgcc ggtggcatct cgccggggcg ctacgcgatc gggctgggcg cggggctggc
     8941 ggtggcggtg ctttcgtcgc tcgtgctcta cgcgatcgtc cggccgttcc tccgccccgg
     9001 acgcgagctc gggtggatcg gcgccgtcgc ggcggtcgcg ttcgccgtcc ggggtgtgct
     9061 cgcatcctcg ttcgcgcgtg agagctacgt cttcccggac ctgctgccgc tgcatcggct
     9121 gggcgacgcg ggcgtcctcc gcctcggcgg cggtgtgacg ctcccgatcc gcacgttctt
     9181 cgtgatcgcc gtcgggttcg cccttgccgc cgcgatggcc cggctgctcg accggtcgag
     9241 ggtgggcgtc gcgttccgcg cgatcgccga ggacccgatg gcggcgcgcg ccgtcggtct
     9301 gcccgtggac gcgctccggt tcgcggcgtt cgcgctcgcg ggaggcgtcg cgatcctcgc
     9361 cgtgatcgtc tcggctccct cggcgccgtt cggagcggcg accggcggtg cgatcgggtt
     9421 gaaggggctg gtggctgcgc tgctcgccgg gttcggttcc ccgtggcggg cgctgggcgc
     9481 cggtgccgca gtcgggatcg tggagacggc tgtctcggtc tcgccgctcc cgctcatccc
     9541 gggggagctg cgggatgtcg tcccgttgct gctcgcgttg gtcctcttgg ccgggcgccg
     9601 ccgtacggcg tcgctggagg tctcctgagc gcggcgcgcg gggccgcatg ggccgccggg
     9661 ctcgtcgtgg tggccgtcgt cccgccagcg ctcgcgggtc cggtcgtgct cacggcgacg
     9721 gcgtcggcgg cctacctcgc gctcgccgcc gtcgggttga acctctcggt gggtctggcg
     9781 gggatgccat cgctcggtca cggagccttc gtcggtctcg gcgcgttcgg tgcagcctgg
     9841 gcgcggacgc gcctcgggtt cgacccggtc gcggccgcgt tggccggcgc ggtgcttgcg
     9901 gctgcggccg gcgcggtcac cggcgccggg ctggcgaagc tccgccccgt catgctcgcg
     9961 gtctcgacct ggatcctggc ctggctcgtc gtcaccgtcc tcgcggcgtt cccggggctc
    10021 tccggcggcg cccgggggat ccccctcccc gagggcgtgc tcgtgctcga ggggatcgac
    10081 cggctgatcc gcttcacccc cggcgtgcat cacggcgtcg ccgtcgtcct cctcggcgcg
    10141 gcgctcctcg cgtatcgccg aaccgatctc ggccctgcgg gcatccacct cgcggcggcg
    10201 cggggagctc ccgcggccgc cgatgcgctg gggcttccgg ccgaacgtct gcggggcggg
    10261 acgttcgtcg cgtcggcggc cgtcgcgggc gtcgcgggcg ggttctccgt ccagctccac
    10321 ggcgtcgccg acgcgtccgc ctatggcccg ctcctgtcgg tggagctgct cctggccgtc
    10381 ctgatcggcg gcgccggggc gacgctgggg ccggtcgtcg ggaccctcgc gctcgtcacg
    10441 cttccgtccg ccgcccggct cgtcgcgggg ctcctcggcg cggacgcatc ggggttcgag
    10501 gcggtcatca ccgcggtcct gctcctcggg atcctcgccg cgggtggccg ggggctgctc
    10561 ccgccccggc ggggctcggc cgcccgggtg gagccggcgg accccccgcc gccacccacc
    10621 gcgacgctgg aagcgcatga cctcgcacgg tcgttcggcg gggtccgggc cctcgagggg
    10681 gtctcactcc gcgtcgcgac gggcgaggtg cacgccgtga tcgggcccaa cggatcgggc
    10741 aagacgacgc tcctgcggat cctggcgggg gcggaccgtc ccgatgcggg ggaggtccgg
    10801 ctcgatggac ggggtctctc tcaggcaggg gttgtcccgc gcctgcgggc gggcgtggcg
    10861 cgcaccctgc agcggaccat gcccttcggc gcgctccgca ccgaggagca cgtcgcggcg
    10921 ggtgccgcga tccatcgccg gacggggccg ttgcgctggc tgctggcgac ccccgccgcg
    10981 cgccgcgagg accgcgccgt cggggcacac gcgcgcggcg ttctccgctc gctcgggctc
    11041 gagtggctgg aggggcgggg ggtccgctcc ctcgacggcg ccgagcgacg actgatgagc
    11101 ctcgcgaccg ccgtcggcgc cgcgccgtcg atcctcctcg tggacgagcc gtccgcgggg
    11161 atggcgccgc gcgaagctca ggtggtggcc gagatgctcc gccgcctccg ggatcgcggg
    11221 ctcgggctgc tggtcgtgga gcacaacgtc tcgctcgtcc acgaggtcgc cgaccgcgtc
    11281 acggtgctcg aggcgggccg ggtcatcgcg accggcacgc cggccgaggt ggtgcgtgac
    11341 ccgcgcgtcc aggaggccta cctgggacgc accggctaga ctcgcgcgca cggtgcgcct
    11401 cctccccgtc gcgctcgcgc tgtcgctcgc ctcctgcggt ggtggcgctc cggccggtct
    11461 tccgaccgag ctgacggtcg tcgtcaacgc gcccttctcc gccgcgccct gggtggcgga
    11521 cgcgatcgag cgcgggacgc gcctggcggc cgaggagatc aatgcggacg gtgggatcga
    11581 gaccgcgggg gagcggtaca ccctcgcggt gcgcacgatg gacaccggcc tctcgccgca
    11641 gcgagccgtg gagaacgtcc gcacggcggt tgccgacggg gtcctggcga tcgtcgacga
    11701 gggaacgggc gtcgacgctt cgtgggaggt cgcccgcgac gcggacctgc cgatcggcat
    11761 cgtcttccag ggagggatgg gcctcgtcga tcacgaggca cgtccgaacg tcttccggat
    11821 cgcccccacg gaccggggga tcgccttccg ctacgcggag tacctcgtcc cgaaggggct
    11881 gcggatcgcg ttcctccacg acgacaccgg gtacggccag gagggggcgg cggcgctccg
    11941 gcgggcgttc gagtgctgcc ccgaggcggt cgaggaggac ctcacgatcc cggccgacgg
    12001 cgaccccgcg ccgcatgtgc tcgaggc
//
LOCUS       DATLGG010000158         6185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_26518, whole genome shotgun sequence.
ACCESSION   DATLGG010000158 DATLGG010000000
VERSION     DATLGG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26518"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VM638_04570"
     CDS             complement(<1..969)
                     /locus_tag="VM638_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11730.1"
                     /translation="MSLSSSGEQQDGSASEVAISADGRVVAFVSDATNLASGDGNGTR
                     DVFVRDLSAGRTELISSTPSRRTGNAASTAPHLSADGRFVAFSSEASDLVPGDTNGVA
                     DVFVHDRQSGRTERVSISSTGGQGDGSSFDPSISWDGRMVAFASVATDLVAGDANDVS
                     DVFLHDRETGQTARASTEADGDQLTAASRQPALSGDGQHLAFVSDAETLVDGDTNGGP
                     DVFEVHLSSGTIRKASVAGDGRQSDGDIGHPTISSDGRYVGFHSSSHFVPAEPGGWSM
                     YVQDRDQDRGRSFLARVSVSEVGSGGRSDAGPSGISDDGRYAVFTSS"
     gene            1306..1698
                     /locus_tag="VM638_04575"
     CDS             1306..1698
                     /locus_tag="VM638_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11731.1"
                     /translation="MRVWGAVGDLLVEVVARRSEDGFRVQRSREARDRLRSAPVCSGL
                     VREGPAVDLRLVPPVGSPLLDLPLALAALGAAGRLEEAGWVFAHGRLGPDGTVYDGRE
                     AAGTLKDAVEACSGDPLSPSLRAGTIGS"
     gene            1698..1880
                     /locus_tag="VM638_04580"
     CDS             1698..1880
                     /locus_tag="VM638_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11732.1"
                     /translation="MDVLSKHDLKAFEERMELRFDSLRDSLLAQIYCGQRDLVLTLVT
                     VIGIVNGITLAALKLP"
     gene            complement(1881..3065)
                     /locus_tag="VM638_04585"
     CDS             complement(1881..3065)
                     /locus_tag="VM638_04585"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVM11733.1"
                     /translation="MTTSVIVSGVRTPIGKFGGALRDLAGAKLGAIAIREALARAGIQ
                     GDQVDYVIMGQVLQAGTGQITARQAAIEAGIPKEVPAITINKVCLSGLNAVALADQMI
                     RAGEIEVAVAGGMESMSQAPYVLRQARWGARMGDAQLVDTMIYDGLWSTFTDKHMGET
                     SDELNDEFRITREEQDAWSVRSHERAASGWESGRLAKEVVPVEVPQRKGDPVVVEQDE
                     GIRPDTTVEGLAKLRPAFREGGSITAGNASQISDGGAALVVMSKERAQALGLEPLAEI
                     VAHGMSADRYPYLHTVPATAMEGAMKKAGIGAQDLGVVEFNEAFAAVAINATRMLGVD
                     EEIVNPNGGAVALGHPIGMSGARLVLTTMHDLHHRDVELGGAALCGGGGQGDALILRR
                     LS"
     gene            3162..3551
                     /locus_tag="VM638_04590"
     CDS             3162..3551
                     /locus_tag="VM638_04590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVM11734.1"
                     /translation="MAAPTLTGYVGTEFTHAEPGLIRGRLEVKPELLAANGYLHAGTV
                     VALADSCCGFGTVVTLAETDADPRAWTTVELKSNFLGTALEGTIECEARMAHGGRSTQ
                     VWDATVTSAATGKTIALFRCTQLILRR"
     gene            3571..4647
                     /locus_tag="VM638_04595"
     CDS             3571..4647
                     /locus_tag="VM638_04595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVM11735.1"
                     /translation="MITDEPVRGSYAYLEHPSLLSLSGLEQLRLFLDGYFDLAPISRL
                     TGLIGEHAAEGEATFSMPASPWWRSGGGVFLAGVFPYVADAALGLAVYNTLPPATGLA
                     TSELSMNFLRPATVASGRLVAEGKLIQMGRSQGLSECRVTDAEGRLLAHGTSRLIKQP
                     LGFEPMPVTDDFPKRTPPDPSDPDPHERPSEGEVVPLAELNERKWLDIYREWRDGTRP
                     YAPVCHLTGMQIADVEEGTAVWTMPASRWYSSSVGAFYGGALAMLADAAMAGAVGSIV
                     PARSSFGTLDLKVNFLRPVRVDDRPLTARATVVHRGKTIAVATCEVTDADGRRVAIGS
                     TSAMLLPGRQWSEAGEAVPIDEAI"
     gene            complement(4651..>6185)
                     /locus_tag="VM638_04600"
     CDS             complement(4651..>6185)
                     /locus_tag="VM638_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM11736.1"
                     /translation="RSPHMLVRPEHIEAGLDEVKGLDGPVDEIVRTLNVFLGYTTFRN
                     KLGGNPRRGVLFEGPPGTGKTYLAKAMAKQAGVPFLFVSAPAFQSMWFGMTNARIRSF
                     FKALRKAARREGGAIGFIEEIDAIGVDRGTSAASPAPAGLGRSVSSMVSSGTGGMVNE
                     LLIQMQSFDQPVWRHKVRDRFVEWVNGYLPAGKRIRTRQPDYHNILLIAATNRGDALD
                     AALLRPGRFDRRLYFDVPTKQGRRDLIDYFLDRKAHDEGLDDEDVRERLAHDTFGYTP
                     VMIEHLFDEALLVALRNNRDKMRPSDVYEAKLSEEIGLRQPVAYTVAERNAVATHEAG
                     HATVAHFLAPTRRLEVLSIIKRRQSLGLLAHGDTEERWTKSRTELEASIAVALGGMAA
                     EELFFGEPGTGPASDLAGATQTAALMVGALGMAGSLVSYEAVNEGPISTRNLVGRVLG
                     DGDGKRKVEDLLDDQKARAIQVLEQNRDVVEALRDALLERDELIGEEIVAEIRAALAR
                     RN"
BASE COUNT          857 a         2200 c         2178 g          950 t
ORIGIN      
        1 cgagcttgtg aacacggcgt acctgccgtc gtcgctgatc ccggagggcc ccgcgtccga
       61 ccggccaccc gatccaacct cggagaccga gacgcgagcg aggaacgaac ggccgcggtc
      121 ctggtcgcgg tcttggacgt acatcgacca gccgccgggc tccgcgggaa cgaagtgcga
      181 ggagctgtgg aacccgacgt agcggccgtc ggagctgatc gtcgggtgcc cgatgtctcc
      241 gtcggactgg cgaccgtcgc cggccaccga ggccttgcgg atcgttccgg aggagaggtg
      301 cacctcgaac acgtcgggcc cgccgttcgt gtccccgtcg accaaggtct cggcgtcgct
      361 cacgaacgcg aggtgctgcc catccccgct cagggcgggc tgccgggagg ctgccgtgag
      421 ctggtcgccg tcggcctcgg tcgaggcgcg tgcggtctgt ccggtctcgc ggtcgtggag
      481 gaagacatcg gagacgtcgt tcgcgtcgcc tgcgacgagg tcggtcgcga cgctggcgaa
      541 cgcgaccatc cgcccgtccc agctgatcga cgggtcgaag gatgacccgt cgccctgccc
      601 gccggtcgat gagatcgaga cccgctcggt ccttccgctc tgccggtcgt ggacgaagac
      661 gtcggccacc ccgttcgtgt ccccggggac caggtcggat gcctcgctgg agaacgcgac
      721 gaagcgtccg tcggcggaga ggtgaggggc ggtggacgcc gcgttgcctg tccggcgcga
      781 cggcgtcgac gaaatcagct cggtccgccc cgccgagaga tcccgcacga acacgtcgcg
      841 ggttccgttc ccgtcgccgc tggcgaggtt cgtcgcgtcg ctcacgaacg ccaccacacg
      901 cccgtcagca ctgatcgcga cctcagaggc cgacccatcc tgctgctccc cggaactgct
      961 cagcgacacc agtcgcgtcg tcgtgggccg gctccacgcc tgcgctgaag gcgccgcggt
     1021 gatgagcaac gtcagcgacg tcgcgatggc cgccgcgcga ggagtaggcg aggaaagcag
     1081 gccagcgaag acgccgtccg cgcgaacacc ccccattgat cgaagtctcc tcaacgaagc
     1141 tccacggctg cttcccctgc tgagcggtcg cccgtcctat tcggtgccag cggtgaggtt
     1201 ccctacgtga tccagcggcg gataggccgc cagtcaagat ccgagtccgt gtcggtgggt
     1261 ggggctaggg ttctgggcgc tcccccacga gccgaggagg cgggcatgcg cgtgtggggt
     1321 gcggtcgggg atctcttggt cgaggtggtg gcccggcggt cggaggacgg gttccgggtc
     1381 cagaggtcgc gggaggcgag ggaccgactg cggtcggcgc ccgtctgctc cggactggtc
     1441 cgggagggac cggcggtgga cctgcgcctg gtcccgccgg tgggctcgcc tctgctggac
     1501 ctcccgctgg ccctggccgc gctcggggcg gcgggtcggc tcgaggaggc gggctgggtg
     1561 ttcgcccacg ggaggctcgg gcccgacggg accgtctacg acgggcgtga ggccgcgggg
     1621 acgctgaagg acgccgtcga ggcctgctcc ggagacccct tgtccccctc tctcagagca
     1681 ggcacgattg gctcttgatg gacgtcctca gcaagcacga tctcaaggcc ttcgaggagc
     1741 gcatggagct ccggttcgac agcctgcgcg actcgttgct ggcgcagatc tattgcggtc
     1801 agcgcgacct ggtgctcacc ctcgttaccg tgatcgggat cgtcaacggg atcacgctgg
     1861 cggcgctgaa gctcccctag ctacgaaagc cggcgcagga tgagggcgtc gccctggccg
     1921 ccgccgccgc agagggcggc gccgccgagc tcgacgtcgc ggtggtggag gtcgtgcatc
     1981 gtggtcagga ccaggcgggc gccgctcatg ccgatggggt ggccgagggc gaccgcgccg
     2041 ccgttggggt tcacgatctc ttcgtcgacg ccgagcatcc gcgtggcgtt gatcgccacc
     2101 gcggcgaacg cctcgttgaa ctcgacgacc ccgaggtcct gggcgccgat gccggccttc
     2161 ttcatcgcgc cctccatcgc ggtcgccggg accgtgtgca ggtacgggta gcggtcggcg
     2221 ctcatgccgt gcgcgacgat ctcggccagc ggctcgaggc cgagcgcctg ggcgcgctcc
     2281 ttcgacatga cgaccaaggc cgctcccccg tccgagatct gcgacgcgtt ccccgccgtg
     2341 atggacccac cttcgcggaa ggccgggcgg agcttcgcca gcccctcgac cgtcgtgtcg
     2401 gggcggatcc cctcatcctg ctcgacgacg accgggtcac ccttgcgctg cggcacctcg
     2461 acggggacga cctccttcgc gaggcggccg ctctcccagc cgctcgccgc ccgctcgtgg
     2521 ctccggaccg accaggcgtc ctgctcctcg cgggtgatcc ggaactcgtc gttcagctcg
     2581 tcggaggtct cccccatgtg cttgtcggtg aaggtcgacc acaggccgtc gtagatcatc
     2641 gtgtcgacga gctgcgcgtc gcccatgcgc gcgccccacc gggcctggcg gagcacgtac
     2701 ggggcctggc tcatcgactc catcccgccg gcgaccgcga cctcgatctc gccggcgcgg
     2761 atcatctgat cggcgagcgc aaccgcgttc aggcccgaca ggcacacctt gttgatcgtt
     2821 atggccggga cctccttcgg gatccccgcc tcgatcgccg cctggcgcgc ggtgatctgg
     2881 ccggtcccgg cctgcagcac ctgccccatg atcacgtagt cgacctggtc gccctggatc
     2941 ccggcgcggg cgagcgcctc gcggatcgcg atcgcgccga gcttcgcccc ggcgaggtcg
     3001 cgcagcgcgc cgccgaactt gccgatcggc gtgcggacgc cggacacgat gacggatgtg
     3061 gtcacgggaa ggctccttta tcctcggtct cccccatgat acggccgccg tcccgacccg
     3121 tccgaggagg aaccgtgatc gagctcgacc gcctgaacga gatggccgcc cccacgctca
     3181 ccggctacgt ggggacggag ttcacccacg ccgaacccgg gctgatccgc ggacgcctcg
     3241 aggtgaagcc cgagctcctg gccgcgaacg gctatctcca cgcggggacc gtcgtcgcgc
     3301 tcgccgactc gtgctgcggc ttcggcacgg tcgtcacgct ggccgagacc gacgccgacc
     3361 cgcgtgcgtg gacgaccgtc gagctgaagt cgaacttcct cggcacggcc ctggagggca
     3421 cgatcgagtg cgaggcgcgg atggcccacg gcgggcgcag cacgcaggtc tgggacgcga
     3481 cggtcacgtc ggccgcgacc gggaagacga tcgccttgtt ccgctgcacg cagctcatcc
     3541 tgcgccgctg acccggatat cctcggcgac gtgatcacgg acgagccggt ccgcgggagc
     3601 tacgcctacc tcgagcatcc ctcgctcctc tcgctctccg gcctcgagca gctccggctc
     3661 ttcctcgacg ggtacttcga cctggcgccg atctcgcggc tgaccggcct gatcggggag
     3721 cacgcggccg agggtgaggc gaccttctcg atgcccgcga gcccgtggtg gcgctccggt
     3781 ggcggcgtct tcctcgccgg cgtcttcccc tacgtcgccg acgccgcgct cggcctcgcc
     3841 gtctacaaca cgctcccgcc ggcgaccggg ctcgccacct cggagctgtc gatgaacttc
     3901 ctgcgcccgg cgaccgtcgc aagcggccgg ctggtggccg aagggaagct gatccagatg
     3961 gggcgctcgc aggggctctc ggagtgccgg gtgacggacg cggagggacg cctgctcgcg
     4021 cacggaacct cgcgtctgat caagcagccg ctcgggttcg agccgatgcc ggtcaccgac
     4081 gacttcccga agcgcacacc cccggacccg tcagaccccg acccccacga gcgcccgtcg
     4141 gagggtgagg tcgtgccgct ggccgagctg aacgagcgga agtggctcga catctaccgg
     4201 gagtggcgcg acgggacgcg gccgtacgca cccgtctgcc acctcaccgg catgcagatc
     4261 gccgacgtcg aggagggcac cgcggtctgg acgatgccgg caagccgctg gtactcgagc
     4321 tccgtcggcg ccttctacgg cggcgcgctg gcgatgctcg ccgacgcggc catggcgggc
     4381 gccgtcggat cgatcgtgcc ggcgcggtcg tcgttcggca cgctcgacct caaggtcaac
     4441 ttcctgcgcc cggtccgggt cgacgaccga cccctcacgg cccgcgcgac ggtcgtccac
     4501 cgcggcaaga cgatcgccgt cgccacgtgc gaggtgaccg acgccgacgg ccggcgcgtc
     4561 gcgatcggca gcacgagcgc gatgctgctc ccgggacgcc agtggtcgga ggcgggcgag
     4621 gccgtcccga tcgacgaggc gatctgaggc ctagttcctc ctcgccagcg ccgcgcggat
     4681 ctcggcgacg atctcctcgc cgatcagctc gtcgcgctcg aggagggcgt cccgcagggc
     4741 ctcgacgacg tcacggttct gctcgaggac ctggatcgcg cgcgccttct ggtcgtcgag
     4801 gaggtcctcg accttccgct tgccgtcgcc gtcgcccagc acgcgcccga cgaggttccg
     4861 cgtggagatc ggtccctcat tcacggcctc gtaggagacg agcgaccccg ccatcccgag
     4921 cgcgcccacc atgagggccg cggtctgggt cgcaccggcg aggtccgaag ccggtccggt
     4981 gcccggctca ccgaagaaca gctcctcggc cgccatgccg cccagagcga ccgcgatcga
     5041 ggcctcgagc tcggtccgcg acttcgtcca gcgctcctcc gtgtcgccgt gcgcgaggag
     5101 gccgagtgac tgccggcgct tgatgatcga gaggacctcg agtcgccgcg tcggggcgag
     5161 gaagtgcgcg accgtcgcgt gccccgcttc gtgcgtggcc accgcgttgc gctccgcaac
     5221 ggtgtaggcg acgggctggc gcaggccgat ctcctccgag agcttcgcct cgtacacgtc
     5281 ggacggtcgc atcttgtcgc ggttgttccg cagggcgacg aggagcgcct cgtcgaagag
     5341 gtgctcgatc atcaccggcg tgtacccgaa ggtgtcgtgc gcgagtcgct cgcgcacgtc
     5401 ctcgtcgtcg agcccttcat cgtgcgcctt gcggtcgagg aagtagtcga tcaggtcgcg
     5461 ccggccctgc ttcgtcggga cgtcgaagta gaggcgacgg tcgaagcgcc ccggccgcag
     5521 gagcgccgcg tcgagggcgt caccgcggtt cgtcgccgcg atcagcagga tgttgtggta
     5581 gtccggctgc cgcgtccgga tccgcttgcc ggccggcagg tagccgttca cccactcgac
     5641 gaagcgatcg cggaccttgt ggcgccagac cggctggtcg aacgactgca tctggatcag
     5701 aagctcgttg accatgccgc cggtcccgga ggagaccatg ctgctcaccg aacgaccgag
     5761 gccggcggga gcgggcgaag cggcggaggt tccgcggtcc acgccgatcg cgtcgatctc
     5821 ctcgatgaag ccgatcgccc ccccctcacg acgagcggcc ttccgcagcg ccttgaagaa
     5881 cgagcggatc cgggcgttcg tcatcccgaa ccacatggac tggaaggcgg gagcggagac
     5941 gaacaggaac ggcacgcccg cctgcttcgc catcgccttc gctaggtatg tcttgccggt
     6001 ccccggtggg ccctcgaaca ggacgccgcg acgagggttg ccgccgagct tgttgcggaa
     6061 ggtcgtgtag ccgaggaaga cgttgagggt gcgcacgatc tcgtcgacgg gaccgtcgag
     6121 gcccttcacc tcgtcgagcc cggcttcgat gtgctcgggc cggacgagca tgtgcggtga
     6181 gcggc
//
LOCUS       DATLGG010000159         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_180187, whole genome shotgun sequence.
ACCESSION   DATLGG010000159 DATLGG010000000
VERSION     DATLGG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180187"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1552)
                     /gene="fumC"
                     /locus_tag="VM638_04605"
     CDS             complement(152..1552)
                     /gene="fumC"
                     /locus_tag="VM638_04605"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVM11737.1"
                     /translation="MSDEARVETDAMGPIEVPADRYWGAQTQRSLHHFSIGDDRMPVE
                     VVRAFGILKKACAEVNADLGRLPREKAEPIVAAAQEVIDGKLDEHFPLYVWQTGSGTQ
                     SNMNANEVIANRAIELTGGVLGSKDPIHPNDHVNMSQSSNDTFPTAMHVAAARAMVER
                     LIPSVRALREALDARAREWTDLVKIGRTHLQDAVPVTLGQEFSGYVAQLDADLARLEA
                     SLDGLYELAIGGTAVGTGLNAPAGFGEKVAARIAQQTGLPFRAAGNFFAALAAHDAMV
                     AAHGAMRTLATSLMKIANDIRWLGSGPRSGLGELELPENEPGSSIMPGKVNPTQSEAM
                     TMVCTQVFGNDVAITVAGSQGNFELNVFKPVIVRNFLHSVAILSDVCRTFREFCVEGI
                     RPNQERIAELVDRSLMLVTALSPHIGYDKAAEIAKKAHHDRRTLRETALELGYVTEEQ
                     YDEWVRPDRMVGPDAG"
     gene            1669..2046
                     /locus_tag="VM638_04610"
     CDS             1669..2046
                     /locus_tag="VM638_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVM11738.1"
                     /translation="MIGKTWMATASVVLLTACGGGGAGGGGDAASPAGGGGDAAASTG
                     TVRMVDNAYEPASMTLDAGQQVELVNDGVAPHTFTIEGEGVDHDVPAGESATETIELE
                     PGTYTVFCKYHREQGMEGTLTIR"
     gene            2043..2822
                     /locus_tag="VM638_04615"
     CDS             2043..2822
                     /locus_tag="VM638_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVM11739.1"
                     /translation="MRALALAMLLPGLAACTASGPATTPVAGPTGRPTPGPTASATAA
                     PAEGSDATEAGGGKVDPRDGGFEVGLGEWAVTLEAEEIRPGPVTFVVRNGGELIHGFE
                     MEIEGVEGDSSGPGSGSAFKHETETFGPGESVRFDLDLAPGLYKVECLVDGHDDMGME
                     AMLRVRRGAPLVAAPSAQAGTVSIAGFRFAPARIEVQPGEEITWTNDDAADHTVTAED
                     GSFGSDPFGRGGTFRWTFDEPGRYAYLCAIHPAMRGEVVVG"
BASE COUNT          412 a         1022 c         1119 g          448 t
ORIGIN      
        1 cgtggagctg gacccagacc tcctcgtccg gacccgctcc ctgcttgtgc gcgggccccg
       61 gacggtcgcg cgtttcgatc gcgtatccga tcgacggtcg tgcggcgctc ccggtcaggt
      121 gctcgaggcg gcggacgacg acgtgctcca tctagccggc gtcgggcccg accatccggt
      181 cgggacgcac ccactcgtcg tactgctcct cggtgacgta cccgagctcg agcgccgtct
      241 cccgcagggt gcgacggtcg tggtgggctt tcttcgcgat ctcggcggcc ttgtcgtagc
      301 cgatgtgcgg cgacaacgcg gtcacgagca tgagcgagcg atcgacgagt tcggcgatgc
      361 gctcctggtt cgggcggatc ccctcgacgc agaactcgcg gaacgtccgg cagacgtcgc
      421 tgaggatcgc gaccgagtgg aggaagttgc ggacgatcac cggcttgaag acgttcagct
      481 cgaagttgcc ctgcgagccg gcgacggtga tcgcgacgtc gttgccgaag acctgggtgc
      541 agaccatcgt catcgcctcg gactgcgtcg gattcacctt ccccggcatg atcgaggagc
      601 cgggctcgtt ctcgggcagc tcgagctcgc cgagacccga ccgtggcccg gacccgagcc
      661 agcggatgtc gttcgcgatc ttcatgaggg aggtggcgag cgtgcgcatc gcgccatggg
      721 cggcgaccat cgcgtcgtgc gcggcgagcg cggcgaagaa gttgcccgcc gcccggaagg
      781 ggagtcccgt ctgctgggcg atcctcgcgg cgaccttctc gccgaagccg gcgggagcgt
      841 tcaggcccgt gccgaccgcc gtgccgccga tcgcgagttc gtacagcccg tcgagcgagg
      901 cctcgagccg ggcgaggtcc gcgtcgagct gggcgacgta gcccgagaac tcctgcccga
      961 gggtcaccgg cacggcatcc tggaggtgcg tccgcccgat cttgacgagg tcggtccact
     1021 cgcgcgcccg cgcgtcgagc gcctcacgga gagcacggac gctcgggatc agacgctcca
     1081 ccatcgcccg cgcggcagcc acgtgcatcg cggtcgggaa cgtgtcgttc gacgactgcg
     1141 acatgttgac gtggtcgttc gggtggatcg gatccttcga tccgaggacc ccgccggtga
     1201 gctcgatcgc gcggttcgcg atcacctcgt tggcgttcat gttcgactgc gtccccgagc
     1261 ccgtctgcca cacgtacagc gggaagtgct cgtcgagctt gccgtcgatg acctcctggg
     1321 ccgcggcgac gatcggctcg gccttctctc gcgggaggcg tccgaggtcc gcgttcacct
     1381 cggcgcaggc cttcttgagg atgccgaagg cgcggacgac ctcgacgggc atccggtcgt
     1441 ccccgatcga gaagtggtgg agggatcgtt gggtctgggc cccccagtag cggtcggccg
     1501 ggacctcgat cggccccatc gcgtcggtct cgacccgcgc ctcgtcgctc acgtccctag
     1561 cgtatccgct gcgacggccg aagcttgacc gcgcgtttgc gccgccttga ccggtcctta
     1621 accggccccc tgccacgatc gtcgcgtccg ctacggaagg aggcagggat gatcggaaag
     1681 acctggatgg cgaccgcgag cgtcgtgctg ctgacggcgt gcgggggcgg aggagccggc
     1741 ggaggcggcg acgccgcgtc gcccgccggc ggaggaggcg acgcggccgc gtccaccggg
     1801 accgttcgga tggtggacaa cgcgtacgag cccgcgtcga tgacgctcga cgcggggcag
     1861 caggtggagc tcgtgaacga tggagtggcg ccgcacacct tcacgatcga gggcgagggc
     1921 gtcgaccacg acgttcccgc gggcgagagc gcgacggaga cgatcgagct cgagcccggc
     1981 acctacaccg tgttctgcaa gtaccaccgc gagcagggca tggaagggac cctgacgatc
     2041 cgatgagggc gctggcgctg gcgatgctgc tgccgggcct cgccgcctgc acggcctccg
     2101 ggccggcgac cactccggtc gccggcccga cgggccgtcc caccccgggc ccgaccgcct
     2161 ccgcgaccgc ggcgccggcc gaggggtcgg acgccacgga ggccggcggc gggaaggtgg
     2221 acccccgcga cggaggcttc gaggtcgggt tgggggagtg ggcggtgacg ctcgaggccg
     2281 aggagatccg ccccggcccg gtcacgttcg tcgtccggaa cggcggtgag ctgatccacg
     2341 ggttcgagat ggagatcgag ggggtcgagg gggacagctc cggccccggc agcgggagcg
     2401 cgttcaagca cgagaccgag accttcggcc ccggcgagag tgtgcgcttc gacctcgacc
     2461 tcgcacccgg tctctacaag gtcgagtgcc tggtcgacgg gcacgacgac atggggatgg
     2521 aggcgatgct ccgggtccgg cgcggggcgc ccctcgtcgc ggccccctcg gcgcaggcgg
     2581 ggaccgtctc gatcgcgggc ttccggttcg ctccggcgcg gatcgaggtc cagccggggg
     2641 aagagatcac ctggacgaac gacgacgcgg ccgaccacac cgtcaccgcg gaggacggtt
     2701 cgttcggctc ggatccgttc gggcggggcg gcaccttccg ttggaccttc gacgagcccg
     2761 gtcgctacgc ctacctctgc gcgatccatc ccgcgatgcg cggggaggtc gtggtcgggt
     2821 gagccgtcgc gcggccgcgc gggcgaggga ggctccgtag gatggccctc gtgccgacgc
     2881 gcgtcctcgt cgtcgaagac gaccgctcga tcgccgagcc cctcgtggat gccctcgtcc
     2941 gcgaggggtt cgaggcggag gcggcgggga cgctcgcgga cgcgcgcgcg tcgttcggcg
     3001 t
//
LOCUS       DATLGG010000160         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_167384, whole genome shotgun sequence.
ACCESSION   DATLGG010000160 DATLGG010000000
VERSION     DATLGG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167384"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1790)
                     /locus_tag="VM638_04620"
     CDS             complement(156..1790)
                     /locus_tag="VM638_04620"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVM11740.1"
                     /translation="MKQPKYIFVTGGVSSSLGKGITSASLGRLLRSRGFRISMQKLDP
                     YINVDPGTMNPFEHGEVFVLDDGAETDLDLGHYERFTDQSLHRGSNVTTGAVYSTVIA
                     KERRGEFLGKTVQVIPHITNEIKDRIRSHASGSGADIALVEIGGTVGDIESLPFLEAV
                     RQLRNEVGRQNCAFVHLSLLPHIGPTGELKTKPTQHSVKELRSLGLQPDVIVCRSDRP
                     IGRGLKEKISLLCDVPMQGVVSAVDADSIYEVPLVLHAEGLDTYLVELLQLPSATPDL
                     TEWSGLVERVQTASAPLTVAVVGKYVDLPDAYLSVTEALRHGGFHHGADVRLRWIASD
                     ELAAGNVAEVLGDADAILVPGGFGVRGVEGKVDAIRFARERKVPFLGICLGLQCAVIE
                     FARNVCGISDANSSEFEPTTPNPVIDLLPEQRDITDLGATMRLGAQPCHVGEGTLAEQ
                     AYGERVVYERHRHRYEVNPAYHEVLRAHGMVFSGLSPDERLVEIIELPDHPYFVAGQF
                     HPELKSRPTRPHPLFRDFIGAAVVRRREAASAPAGS"
     gene            complement(1861..2676)
                     /locus_tag="VM638_04625"
     CDS             complement(1861..2676)
                     /locus_tag="VM638_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11741.1"
                     /translation="MTRVGLAAIGGAGLVWGAWVLLVRGRLVPRRPNHRGRSVPTGVG
                     LSLAFAVVAAAAARMVAGGSGATPVEVTAGWSLLALAAAGFVDDRVVGGPRGLPGHVR
                     SLLRGRMTSGILKLVVAIAAAAWVAATARPDPLGFGLALVVLAAATNVWNAFDVRPGR
                     ALKWSLCALVPMLVASWGETFSFLAAAMIGGCLVLLVPDLRERTMLGDAGSNPLGFAV
                     GLGLVSTLDLLGLAVAAVLLLALQVVAETVTITRVIERVPPLRWFDRLGRLPR"
     gene            complement(2673..3341)
                     /locus_tag="VM638_04630"
     CDS             complement(2673..3341)
                     /locus_tag="VM638_04630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM11742.1"
                     /translation="MKILALIAAYEGSGMIAATVRAVRSLGDVSEVVVVDDGSTDETA
                     ERAAAAGARVLRAPRNLGKGGALEGALARLPSVDVVLLLDQDLGDTAAAASSLLGPVV
                     EGEADMTIAQLPRDPRHGGFRLVKRLSGWMIRRLCGFPPQEPMSGQRAVSARALEAAR
                     PLAPGFGVELGMTVDVVRAGMRVVEVPVEMSHAPTGRDLRGFLHRARQGRDHVRAALA
                     RVRP"
     gene            complement(3338..>4099)
                     /locus_tag="VM638_04635"
     CDS             complement(3338..>4099)
                     /locus_tag="VM638_04635"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HVM11743.1"
                     /translation="REQVVDLQSERQTWSAFGGEVAAYLVDGRLPAREVVIVTQEGTD
                     TLAIDAARRALQAAGAEVAAVLSVDPSLSLEREADVTEVARLVGSPHEDPEAVAADAA
                     AALAARLVSGSGPTDVLEGFLASGFLLNRGPGLGAAAVRALGGRDQIFLMVAGGEDEP
                     AVDPAWFLAPLTRELARSGASVGAAEPADTEYSFVGILRGDAAVSRLLVTQDNVDQVP
                     GEVGLVLGLQDLVVNGRPGHYGVKDGADAVIPPLP"
BASE COUNT          644 a         1512 c         1435 g          508 t
ORIGIN      
        1 ccacagaggt caaggacaac gaatttcgcg tcgcggtcac gccggccggc gtgaccgcga
       61 cgcgaaattc gttgtccttg acctctgtgg ggatcccgac gatcaacgcg cccccttccg
      121 gacggccggc ctaggatacc gctccgcgac ggcggctagg agcccgcggg cgcagacgcg
      181 gcctcgcgcc tgcggacgac cgcggccccg atgaaatccc ggaacaacgg gtgcggccgc
      241 gtcggccgcg acttcagctc cgggtggaac tggcccgcga cgaagtatgg gtgatccggg
      301 agctcgatga tctcgacgag gcgctcgtcc ggcgacagcc ccgagaagac catcccgtgg
      361 gcccgcagga cttcgtggta cgccgggttc acctcgtagc ggtgacggtg ccgctcgtag
      421 accacgcgct ccccgtaggc ctgctcagcc agggttccct ccccgacgtg acacggctgg
      481 gcgccgaggc gcatcgtcgc gccgaggtcc gtgatgtcgc gctgctcggg caggagatcg
      541 atcacggggt tgggggtggt cggctcgaac tcgctcgagt tcgcatccga gatcccgcac
      601 acgttccgcg cgaactcgat caccgcgcac tgcagcccca ggcagatccc gaggaagggg
      661 accttccgct cgcgcgcgaa gcggatcgcg tcgaccttcc cctcgacgcc gcgcaccccg
      721 aacccccccg ggacgaggat cgcgtcggca tcgcccagaa cctcagccac gttgcctgcc
      781 gccagctcgt ccgaggcgat ccagcgcagc cggacgtccg caccgtggtg gaagccgccg
      841 tgccgcaggg cctccgtcac cgacaggtaa gcgtcgggaa ggtcgacgta cttgccgacc
      901 accgcgaccg tcagtggcgc gctcgcggtc tgcacgcgct ccacgaggcc cgaccactcg
      961 gtgaggtccg gcgtcgcgga cgggagctgg aggagctcga ccaggtaggt gtccagaccc
     1021 tcggcatgca ggacgagcgg gacctcgtag atggagtccg catcgacggc cgagaccacg
     1081 ccctgcatcg ggacgtcgca gagcagggag atcttctcct tcaggccgcg cccgatcggc
     1141 cggtcggagc ggcacacgat cacgtccggc tgcaggccga gggatcggag ctccttgacc
     1201 gagtgctgcg tcggcttcgt cttcagctca ccggtcgggc cgatgtgcgg gagcagcgag
     1261 aggtgaacga acgcacagtt ctgccgcccg acctcgttcc ggagctgccg gaccgcctcg
     1321 aggaagggca gcgactcgat gtcgccgacg gtgccgccga tctccacgag ggcgatgtcg
     1381 gcgccggacc cgctcgcgtg gctgcggatc cggtccttga tctcgttggt gatgtgcggg
     1441 atcacctgga ccgtcttgcc gaggaactcg ccgcgccgct ccttcgcgat cacggtcgaa
     1501 tagaccgcgc cggtcgtgac gttcgagccg cggtggaggc tctggtcggt gaaacgctcg
     1561 tagtgcccga ggtcgaggtc ggtctcggcc ccgtcgtcga gcacgaagac ctcgccgtgc
     1621 tcgaacggat tcatcgtccc gggatcgacg ttgatgtacg ggtcgagctt ctgcatcgag
     1681 atgcggaagc cgcgcgatcg gaggagccgt ccgagggagg cggaggtgat gcctttcccg
     1741 aggctcgagg agacgcctcc cgtgacgaag atgtacttcg gctgtttcac ctcgggactc
     1801 gatgctagca gaggttcccc ccaccgagaa gcaccacgcc cgatatctcc cgaagaacct
     1861 tcaacgtggg aggcgaccga gccggtcgaa ccagcgcagg ggcgggacgc gctcgatgac
     1921 gcgagtgatc gtcacggtct ccgcaacgac ctggagggcg agcaggagga cggcggcgac
     1981 ggcgagcccc aggaggtcca gcgtcgagac caggccgagc ccgaccgcga agccgagcgg
     2041 gttcgacccg gcgtccccga gcatcgtccg ctcccgcagg tcgggaacga gcaggacgag
     2101 gcagccgccg atcatcgccg ctgcgaggaa ggagaaggtc tcgccccagc tcgccacgag
     2161 catcggcacg agcgcgcaga gcgaccactt cagcgcgcgg cccggccgga cgtcgaaggc
     2221 gttccagacg ttcgtcgcgg cggcgaggac gacgagggcg agcccgaagc cgagcggatc
     2281 agggcgcgcc gtggccgcca cccacgccgc cgcggcgatg gccacgacga gcttcaggat
     2341 ccccgacgtc atccgcccgc ggagcaggct ccggacgtgg ccggggaggc cgcgcggtcc
     2401 cccgacgacg cggtcgtcga cgaacccggc tgcggcgagc gcgaggagcg accatccggc
     2461 cgtcacctcg accggggtcg ccccggaccc gccggccacc atccgcgccg ccgccgcggc
     2521 cacgacggcg aacgcgaggg agagccccac gcccgtcggg accgagcgtc cccggtggtt
     2581 cggccgccgg ggaaccagcc tgcctcgcac gagcaggacc catgcgcccc agaccaagcc
     2641 ggcgccgccg atggccgcga gaccgacccg cgtcacggtc ggacgcgcgc gagggccgcc
     2701 cgcacgtggt ccctcccctg ccgcgcccgg tgcaggaacc cacggaggtc gcggccggtc
     2761 ggcgcgtggg acatctcgac cggcacctcg acgacgcgca tgccggcccg cacgacatcg
     2821 accgtcatcc cgagctcgac gccgaaccca ggcgccagcg gccgcgccgc ttccagcgca
     2881 cgggccgaaa cggcgcgctg gcccgacatc ggctcctgcg gagggaaacc gcacagacgg
     2941 cggatcatcc accccgagag ccgcttcacg aggcggaagc cgccgtgacg cggatcgcgc
     3001 ggcagctgcg cgatcgtcat gtcggcctcg ccctccacga ccggcccgag caacgacgag
     3061 gccgctgccg ccgtgtcgcc gaggtcctgg tccagcagga ggacgacgtc gacggatggc
     3121 agccgcgcga gcgcgccctc gagcgccccg cccttgccga ggttgcgggg ggcacggagg
     3181 acccgagcgc ccgcggcggc tgcgcgctca gccgtctcgt ccgtcgagcc gtcgtccacc
     3241 acgaccacct cggagacgtc tccgaggctc cgcacggcgc ggaccgtcgc agcgatcatg
     3301 ccgctcccct cgtaggcggc gatgagggcg aggatcttca cggcagcggg gggatcaccg
     3361 cgtcggcccc gtccttcacg ccgtagtgac cgggccggcc gttcacgacg aggtcctgaa
     3421 ggccgaggac gagcccaacc tcccccggca cctggtcgac gttgtcctgc gtgacgagga
     3481 gcctcgagac ggccgcgtca ccgcgcagga tcccgacgaa cgagtactcg gtgtcagcgg
     3541 gctccgccgc gccgacgctg gcacccgagc gagccagctc cctggtcagc ggggcgagga
     3601 accacgcggg atcgacggcc ggctcgtcct cgccaccggc gaccatcagg aagatctgat
     3661 cgcggccgcc gagcgccctc acggccgccg cgccgaggcc gggtccgcgg ttcaggagga
     3721 accccgaggc gaggaacccc tcgaggacgt ccgtcggtcc gctcccactg acgaggcgag
     3781 ctgccagcgc tgcggcagcg tcggccgcca cggcctccgg atcctcgtgc gggctgccga
     3841 cgagccgcgc cacctccgtg acatcggctt cccgctccag cgacaggctc gggtcgaccg
     3901 acagcaccgc ggcgacctcc gcgcccgccg cctggagagc gcggcgggcc gcgtcgatcg
     3961 cgagggtgtc cgtcccttcc tgggtcacga tgacgacctc ccgcgccgga aggcgcccgt
     4021 cgaccaggta cgccgccacc tcgccgccga aagccgacca cgtctgacgc tccgactgga
     4081 ggtcgacgac ctgctcccg
//
LOCUS       DATLGG010000161         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_180210, whole genome shotgun sequence.
ACCESSION   DATLGG010000161 DATLGG010000000
VERSION     DATLGG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180210"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VM638_04640"
     CDS             complement(<1..972)
                     /locus_tag="VM638_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM11744.1"
                     /translation="MAQVINEPAGDPLGAGAPGEPGQQVVGRSPGQLFWRRFRRDRFA
                     IAGLIFLGFIAIMALAAPLAAAITGHGPNETFLREATDEVGLPLGPSRDFWMGANRSG
                     QDLFVRVVYGARTSLTVALLATGISLVIGVTLGVAAGFYRGKTDTVISRLIDIVLSLP
                     VLLLALGLVAACGLSRQGCLGGLIRPGMLLVSYVIGLFSWPYIARIVRGQVLSLREKE
                     FIEAARSLGAGDLRIMTREILPNVVAPIIVYTTLIIPNNILFEAALSFLGIGVPPTTP
                     SWGRMLSDASVQGIYQVAPWMMLFPGLFLFLTTMAFNLVGDGLRDALD"
     gene            complement(1072..>2034)
                     /locus_tag="VM638_04645"
     CDS             complement(1072..>2034)
                     /locus_tag="VM638_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HVM11745.1"
                     /translation="IEGEEIIDRRVGRDLTSQESQALAAEVNEKLTLPEATFELKATV
                     EHRGALVIRSAKGPLSAEVGNTDPAYVKQGPLGVALETFPNRVARSEPLEETEAARLG
                     AELTNRFVEGARRVLEASEVNANRRAAGKLPGNLILTRDAGDHIPRLQPIRDRFGPSW
                     GCFVEMPVERGIAMVLGMAEVPAPTPDDPEERYAAWAALAAEALGGHDALYIHIKGPD
                     VPAHDGRAEDKRTVCEQIDGAFFGELLPRIDPSSTLVAVTADHATSCARKAHTDDPVP
                     LLVSGPGVSADAPDVFGERAAAAGALGTMNGVDIVPALVELLRR"
BASE COUNT          302 a          752 c          710 g          270 t
ORIGIN      
        1 gtccagcgcg tcccggaggc catccccgac caggttgaac gccatcgtcg tcaggaagag
       61 gaacaggcca gggaacagca tcatccacgg ggccacctgg tagatcccct gcaccgaggc
      121 gtccgagagc atccgccccc acgatggggt ggtcggcggc acgccgatcc cgaggaagga
      181 gagcgccgcc tcgaacagga tgttgttcgg gatgatcagc gtcgtgtaga cgatgatcgg
      241 cgcgacgacg ttcggcagga tctcgcgcgt catgatccgc aggtcccccg ccccgagcga
      301 ccgggccgcc tcgatgaact ccttctcccg gagcgacaac acctgacccc ggacgatccg
      361 ggcgatgtag ggccagctga acaggccgat gacgtagctg acgagcagca tgcccgggcg
      421 gatcagcccg ccgaggcagc cctgccggct caggccgcac gccgcaacga ggccgagcgc
      481 gagcaggagg acgggcagcg agagcacgat gtcgatcagc cgcgagatga ccgtatccgt
      541 cttcccccgg tagaagccgg ccgcgacccc gagggtcacc ccgatgacca gcgagatccc
      601 cgtcgcgagc agggcaacgg tcagcgaggt gcgggcgccg tacacgacgc gcacgaagag
      661 gtcctggccg gagcggttcg ctcccatcca gaaatcgcgg ctcggcccga gcggcagccc
      721 cacctcgtcc gtcgcctccc gcaggaacgt ctcgttcggc ccgtgccccg tgatggcagc
      781 ggcgagcggc gcggcgaggg ccatgatcgc gatgaacccg aggaagatga gccccgcgat
      841 cgcgaagcgg tctcggcgga agcgccgcca gaacagctgc cccgggctcc ggccgacgac
      901 ctgctgcccg ggctccccgg gcgcgcccgc gcccagcggg tcgccggcgg gctcgttgat
      961 cacctgcgcc actcggtccc ttcctcagcg acgtgccgca tcctagccgc ggcgcccggc
     1021 gcgggcaacg agcgccccgg tccatgtacg gatcgggggc cgggccggat cttacctccg
     1081 caggagctcg accagggcgg gaacgatgtc gaccccgttc atcgtcccga gggcccccgc
     1141 cgcggccgcg cgctctccga agacatccgg agcgtccgcc gaaacgcccg ggccggacac
     1201 caggagcggc acggggtcgt ccgtgtgtgc cttgcgggcg caggaggtcg cgtggtcggc
     1261 ggtgacggcg accagggtcg aggaggggtc gatccggggc agcagctctc cgaagaacgc
     1321 cccgtcgatc tgctcgcaga ccgtccgctt gtcctccgct cgcccgtcgt gcgcggggac
     1381 gtccggaccc ttgatgtgga tgtagagcgc gtcgtggccg ccgagggcct cggcggccag
     1441 cgccgcccac gccgcgtacc gctcctccgg atcgtccggt gtcggcgcgg ggacctcagc
     1501 catccccagg accatcgcga tgccgcgttc gacgggcatc tccacgaagc acccccacga
     1561 cggtccgaac cggtccctga tcggctggag gcgcgggatg tggtccccgg cgtcccgcgt
     1621 caggatcagg ttcccgggga gcttcccggc cgccctccgg ttcgcgttca cctcggaggc
     1681 ctcgaggacg cggcgcgcgc cctcgacgaa ccggttcgtc agctcggcgc ccagccgcgc
     1741 ggcctcggtc tcctcgaggg gctcggagcg ggcaacgcgg ttcgggaagg tctcgagggc
     1801 gacccccagc ggtccctgct tgacgtacgc ggggtcggtg ttgccgacct cggcggacag
     1861 cggccccttc gcggagcgga tcacgagcgc gccgcggtgc tccaccgtcg ccttcagctc
     1921 gaacgtcgcc tccggcaggg tcagcttctc gttcacctcc gcggccagcg cctgcgactc
     1981 ctggctcgtg agatcccgcc cgacccggcg gtcgatgatc tcctcgccct cgat
//
LOCUS       DATLGG010000162         6831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_69725, whole genome shotgun sequence.
ACCESSION   DATLGG010000162 DATLGG010000000
VERSION     DATLGG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69725"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VM638_04650"
     CDS             <1..1457
                     /locus_tag="VM638_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HVM11746.1"
                     /translation="TTILAEQHHVTFSERFAPFPVRVAMLSRFLSKAEERRVLEELAA
                     GAVDVIVGTHRLLSKDVRFKDLGLLIVDEEQRFGVAHKERLKKLRVNVDVLSMSATPI
                     PRTLEMALAGIREMSVVDTPPEDRQPVLTSVGPWDEATALGAVRRELRRGGQVFWVHN
                     EVRTIDRRAGWLQTRVPEARILVAHGQMDEANLEKAMIAFWAGEADVLVCSTIIESGL
                     DVPSANTLIVDRADRMGLSQLYQLRGRVGRSAERAFAYLFFPPQASLSEEAHERLTAI
                     GRLTELGSGLRVAMRDLEIRGAGNLLGAEQSGHVAAVGFDTYLRLLGEEVAELRGQPQ
                     PEEREIRVDLPVRAFIPPEWLGQERLRLELYRRVSSSRSDEELDALREETLDRFGAMP
                     PEVETLFRLARLRILCSALGVEEVSTFRGQVRIRPLPLSEEGRVLPEEASYHAATRTL
                     NLDPPPAEGGPRLPGWVRESLLGLVPAPAGTPSG"
     gene            1468..2454
                     /locus_tag="VM638_04655"
     CDS             1468..2454
                     /locus_tag="VM638_04655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVM11747.1"
                     /translation="MRPPRKLLGCVLAVLLLSGCAAFDTAAAVVDGSRIEEEPFARTL
                     EYFAADPQFAAQFPGEEAREGKRSLARQLLTFLIHQVVIEEHARARSLGADEAAVERR
                     LQEQIQALGGEQAFDRQLAAAGASRGDVEDLIRKQVLRENVARLVVTEQVPEEQLRQQ
                     YEERAIEFTQVRVSHILVADEALAQDLAQRVTPKNFAELAREHSEDPGSAEQGGDLGM
                     RPAGEFVEPFARATVGSSVGDIVGPVRTEFGYHIVWVRERTAPAFEEVRDQLLAEVGQ
                     EFFTNWLLQRLRDAQVLVNPRYGVFDRESGEVVERTATTPLPDEQPDVQVQP"
     gene            2454..3305
                     /gene="mazG"
                     /locus_tag="VM638_04660"
     CDS             2454..3305
                     /gene="mazG"
                     /locus_tag="VM638_04660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HVM11748.1"
                     /translation="MGEVRGTPDPLSHVPKGPVPSSKEGERLLDLVRVVARLRGPGGC
                     PWDAAQTHRSLRRHLLDEAYELLDAIDDGDPDRIKDELGDLLLQAIFHAQMAAEAAAF
                     DIDDVAEGTVAKLIHRHPHVFADVDAETPEEVYGNWERIKAEEAGRREIDEGVPAELP
                     SLAAAQKVQRRAAGRGFDWAELAGAVEKVREELAELEEAAAAHGEEDREGAAKDALER
                     EVGDVLFAVSALARRLGVDAEIALRGTTRTFVQRFSTMAERAEAEGVRLEDLSEEELV
                     ARFRAAR"
     gene            3302..4225
                     /locus_tag="VM638_04665"
     CDS             3302..4225
                     /locus_tag="VM638_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVM11749.1"
                     /translation="MTYLAALVGILTISFSAALVAMADVSASTAAFYRCAYAVPFLLV
                     GWLTIRRRDRRTRRERVLAVVAGVLFALDLAFWHRAIEMIGTGMSTLLGNTQILFVGV
                     LAWMFFRERPTRAAMVLIPVVLGGVALSSGLGRGDAFGSDPVLGAVFGLLTGLAYAGF
                     LLIFRASNRSLAPSAGPLLDATAGAAVAGLAFAPFDTDFSFAIPMASHLWLLVLAVGV
                     QVVGWLLISIALPRLPSLETSVLLLLQPLAALLWGILFFGEDPSAVQWVGNALILAGV
                     AVLSWRGSVEETRVERPLDAPGPIHEASTPE"
     gene            4276..5556
                     /gene="eno"
                     /locus_tag="VM638_04670"
     CDS             4276..5556
                     /gene="eno"
                     /locus_tag="VM638_04670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HVM11750.1"
                     /translation="MPPIERVHAREILDSRGNPTVEAEVVLAEGIFGRAAVPSGASTG
                     EHEAVERRDGDAKRFGGKGVLDAVRAVDETIAPALLGIDVLDQRQVDLLMLDLDGTPD
                     KSSLGANAILAVSLATAKAAAAWQGLPLYRYLGGPNAHLLPVPLLNVINGGAHADNSL
                     DVQEFMLAPVGAASFSEALRWGAEVFHALRARLLERGLATAVGDEGGFAPDLPGTAAA
                     CAELVAAIEAAGLEPGEEVALALDVAASELHEDGRYRIEGEERRSEEMSGFYEGILRD
                     FPIVSIEDPLDENDWEGWGALTSALGTRVQLVGDDLFVTHPERLERGMAAGAGTAILV
                     KPNQIGTLTETMDVVRAAAEGGFGRVMSHRSGETEDATIADLAVAGGTGQIKTGAPSR
                     GERTAKYNQLLRIEEELGEGARYAGTAPFRRYAR"
     gene            5553..5873
                     /locus_tag="VM638_04675"
     CDS             5553..5873
                     /locus_tag="VM638_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HVM11751.1"
                     /translation="MSSRAEVGTRPRLHPRSALLGALLLLLAATGIVPVKQYLAQRER
                     MADLERKVELLATERERLRARIAQLQDPAELERLARECLGMVKPGEIAFVGVPEGGGP
                     AADC"
     gene            5998..6360
                     /locus_tag="VM638_04680"
     CDS             5998..6360
                     /locus_tag="VM638_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HVM11752.1"
                     /translation="MIADLEVGAVVEGTVQRITPYGAFLQLEDGRVGLVHISEIDRNY
                     VKDVREHLRENDKVQAKVQSIKEDGKIDLSIKALQDPPRRPLRRGSDPDLEHKLKRFM
                     KQSEERQVDFRRSMDKRR"
     gene            6441..>6831
                     /locus_tag="VM638_04685"
     CDS             6441..>6831
                     /locus_tag="VM638_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HVM11753.1"
                     /translation="MARCGVGHPLVIRNHPLDREGRPFPTLFWLTCPDAVRAVSRLEA
                     DGWIKRLAERAEQEPAFGQELAEAHERHARERGRLASEGEGWGGVAGSPGGVKCLHAH
                     YANHLAGGGDPVGAWVAERVEPVHARGG"
BASE COUNT          930 a         2374 c         2542 g          985 t
ORIGIN      
        1 cgacgacgat cctcgccgag cagcaccacg tgacgttctc cgagcggttc gctcccttcc
       61 ccgtccgcgt cgcgatgctg tcgcgcttcc tgtcgaaggc ggaggagcgt cgggtgctgg
      121 aggagctggc ggccggcgcc gtggacgtga tcgtcggcac gcaccggctc ctgtcgaagg
      181 atgtgcggtt caaggacctc gggctgctga tcgtcgacga ggagcagcgg ttcggcgtgg
      241 cccacaagga gcggctgaag aagctgcggg tgaacgtcga cgtcctttcg atgtcggcga
      301 cgccgatccc gcgcacgctc gagatggcgc tcgccggcat ccgcgagatg agcgtcgtcg
      361 acactccgcc ggaggaccgg cagccggtcc tcacctccgt cgggccgtgg gacgaggcga
      421 ccgcgctcgg ggcggtgcgc cgcgagctgc ggcgaggcgg acaggtcttc tgggtgcaca
      481 acgaggtccg cacgatcgac cgccgtgccg gatggctcca gacccgggtc cccgaggcgc
      541 gcatcctcgt ggcgcacggg cagatggatg aggcgaacct cgagaaggcg atgatcgcct
      601 tctgggccgg ggaggccgac gtgctcgtgt gctcgacgat catcgagtcg ggtctggacg
      661 tgccgagcgc gaacacgctc atcgtcgacc gggccgaccg catggggctc tcgcagctct
      721 accagctccg ggggcgggtg ggccgctcgg cggagcgagc gttcgcctac ctcttcttcc
      781 caccgcaggc gtccctgagc gaggaggcgc acgagcggct gacggcgatc ggccgcctca
      841 ccgagctcgg ttcggggctc cgcgtcgcga tgcgcgacct cgagatccgc ggagccggca
      901 atctcctcgg cgcggagcag tcggggcacg tggccgccgt cggcttcgac acgtaccttc
      961 ggctcctggg tgaggaggtg gcggagctgc gcgggcagcc ccagccggag gagcgggaga
     1021 tccgcgtgga cctcccggtg cgcgccttca tcccgccgga gtggctcggg caggagcgac
     1081 tgcggctcga gctgtaccgc cgcgtgtcgt cctcccgctc ggacgaggag ctcgacgcgc
     1141 tgcgcgaaga gaccctcgac cggttcggcg cgatgccgcc ggaagtcgag accctcttcc
     1201 ggctcgcacg gctgcgcatc ctctgctccg ccctcggggt cgaggaggtc tcgacgttcc
     1261 ggggacaggt ccggatccgg ccgctgcccc tctcggagga ggggcgcgtc ctgcccgagg
     1321 aggcgtcgta ccacgccgcg acgcggacgc tgaacctcga tccgccgccc gccgagggcg
     1381 gtccgcgtct ccccggctgg gttcgggagt ccctcctcgg gctcgtcccg gcgcccgccg
     1441 ggacccccag cggctagaat cccgaccgtg cgccctcccc ggaagctcct cggctgcgtc
     1501 ctggccgtcc tcctgctgtc cggctgcgcc gccttcgata cggcggcggc cgtcgtcgac
     1561 gggtcccgga tcgaggagga gccgttcgcc cggacgctcg agtacttcgc ggccgacccg
     1621 cagttcgccg cgcagttccc cggcgaggag gcgcgggagg gcaagcggag cctcgcgcga
     1681 cagctgctga ccttcctcat ccaccaggtc gtcatcgagg agcacgcgcg ggccaggtcg
     1741 ctcggcgccg acgaggccgc cgtcgagcgt cgcctccagg agcagatcca ggcgctcggt
     1801 ggcgagcagg cgttcgaccg gcagctggcc gcggcgggcg ccagccgggg cgacgtcgag
     1861 gacctgatcc gcaagcaggt gctccgcgag aacgtcgccc ggctcgtcgt caccgagcag
     1921 gtaccggagg aacagctccg ccagcagtac gaggagcgcg cgatcgagtt cacgcaggtg
     1981 cgtgtctcgc acatcctcgt cgcggacgag gcgctcgcgc aggacctcgc gcagcgtgtg
     2041 acccccaaga acttcgccga gctcgcccgc gagcactccg aggatccggg ctcggccgag
     2101 caggggggcg acctcggtat gcgtccggcc ggggagttcg tcgagccgtt cgcgcgggcg
     2161 accgtcgggt ccagcgtcgg cgacatcgtc ggtccggtcc ggaccgagtt cgggtaccac
     2221 atcgtctggg tccgcgagcg caccgccccc gccttcgagg aggtccgtga ccagctgctg
     2281 gcggaggtcg gacaggagtt cttcacgaac tggctcctcc agcggctccg ggacgcgcag
     2341 gtcctggtca acccccggta cggcgtcttc gaccgcgaga gcggcgaggt ggtcgagcgg
     2401 accgccacga cgccgcttcc cgacgaacag cccgacgtcc aggtccagcc gtagtgggcg
     2461 aggtgcgcgg gacgccggat ccgctctcgc acgtcccgaa gggtccggtc ccctcctcga
     2521 aggagggtga gcgactcctc gacctcgtcc gcgtcgtcgc gcgcctccgc ggtcctggcg
     2581 gatgtccgtg ggatgcggcc cagacgcatc ggagcctccg acggcacctc ctcgacgagg
     2641 cgtacgagct cctcgatgcg atcgacgacg gcgatcccga ccggatcaag gacgagctcg
     2701 gggatctgct gctccaggcg atcttccacg cgcagatggc ggctgaggcc gcggcgttcg
     2761 acatcgacga cgtcgccgag ggcacggtgg cgaagctgat ccaccggcac ccgcacgtct
     2821 tcgccgacgt cgacgcggag acgcccgagg aggtctacgg caactgggag cggatcaagg
     2881 ccgaagaggc cgggcgccgc gagatcgatg agggcgtgcc ggcggagctc ccctcgctgg
     2941 ccgccgcgca gaaggtccag cggcgcgcgg cgggacgcgg cttcgactgg gccgagctcg
     3001 cgggcgccgt cgagaaggtg cgcgaggagc tggcggagct cgaggaggcc gccgccgcac
     3061 acggcgagga ggatcgcgaa ggtgcggcga aggacgccct cgagcgggag gtcggcgacg
     3121 tgctgttcgc ggtgtcggcg ctcgcgcggc gcctcggggt ggatgccgag atcgcgctgc
     3181 gggggacgac ccggaccttc gtccagcgct tctccaccat ggcggagcga gcggaggccg
     3241 agggggtccg cctcgaggat ctctcggagg aggagctcgt cgcgaggttc cgggcggcgc
     3301 ggtgacctac ctcgcggcgc tggtcgggat cctcacgatc tcgttctcgg ccgccctcgt
     3361 cgcgatggcc gatgtctcgg cgtcgacggc ggcgttctac cggtgcgcgt acgcggtccc
     3421 gttcctgctc gtcggctggc tgacgatccg tcgtcgcgac cgaaggacac ggcgtgagcg
     3481 ggtgctggcc gtcgtcgccg gcgtcctctt cgcgctcgac ctcgccttct ggcaccgcgc
     3541 gatcgagatg atcgggacgg ggatgtccac gctgctcggg aacacgcaga tcctgttcgt
     3601 cggcgtcctc gcctggatgt tcttccgcga gcggccgacg cgtgcggcga tggtgctgat
     3661 ccccgtcgtc ctcggcggcg tcgcgctctc gagcggcctc ggccgcggcg acgcgttcgg
     3721 gtcggacccc gtgctcggcg cggtcttcgg gctcctgacg ggactcgcct acgcgggctt
     3781 cctcctgatc ttccgcgcgt cgaaccgctc cctcgcaccc tcggccggcc ccctcctgga
     3841 tgcgacggcc ggggcagccg tcgccggact cgcgttcgcg ccgttcgaca cggacttctc
     3901 cttcgcgatc ccgatggcct cgcacctgtg gctcctcgtg ctggcggtcg gcgtccaggt
     3961 ggtcggctgg ctcctgatct cgatcgcgct ccctcggctc ccgtcgctcg agacctcggt
     4021 gctcttgctg ctgcagccgc tcgccgcgct cctgtggggg atcctgttct tcggggagga
     4081 tccgtcggcg gtccagtggg tcggcaacgc gctgatcctc gccggcgtcg cggtgctctc
     4141 gtggcgcggg agcgtcgagg agacccgggt tgagcggccc ctcgatgccc ccggtccgat
     4201 ccacgaggca tcgacgcccg agtagccgcg gaaatccccg ggccgcgcac gctcagcccc
     4261 taggatggtg aggccatgcc gccgatcgaa cgcgtccacg cccgggagat cctcgactcg
     4321 cgggggaacc ccacggtcga ggccgaggtc gtcctcgccg aggggatctt cgggcgggcg
     4381 gccgtccctt ccggcgcctc caccggggag cacgaggccg tcgagcggcg tgacggcgac
     4441 gcgaagcgtt tcggcggcaa aggtgtgctc gacgccgtcc gtgcggtcga cgagacgatc
     4501 gcgccggcgc tgctggggat cgacgtgctc gaccagcggc aggtcgacct tctcatgctc
     4561 gacctcgatg gaacgccgga caagtcgagc ctcggcgcga acgcgatcct cgcggtttcg
     4621 ctcgcgacgg cgaaggcggc cgccgcctgg caggggcttc cgctgtaccg gtacctcggc
     4681 gggccgaacg cccacctgct gcccgtcccg ctcctcaacg tgatcaacgg cggggcccat
     4741 gccgacaact cgctcgacgt gcaggagttc atgctggcac ccgtgggcgc agcgtcgttc
     4801 tccgaggcgc tgcggtgggg cgccgaggtc ttccacgcgc tccgggcgcg cctcctcgaa
     4861 cgcggcctcg ccaccgcggt cggggacgag ggcgggttcg cccccgacct gcccgggacg
     4921 gcagccgcct gcgcggagct cgtcgcggcg atcgaagcgg ccggcctcga gccgggcgag
     4981 gaggtcgcgc tggcgctcga cgtggccgcg agcgagctgc acgaggatgg tcgataccgg
     5041 atcgagggtg aggagcgtcg ctcggaggag atgagcggct tctacgaggg gatcctgcgc
     5101 gacttcccca tcgtctcgat cgaggatccg ctcgacgaga acgactggga ggggtggggg
     5161 gccttgacct ccgcgctcgg gacgcgcgtc cagctggtcg gcgacgacct gttcgtgacc
     5221 catccggagc ggctcgagcg agggatggcg gcgggagccg gcaccgcgat cctggtcaag
     5281 cccaaccaga tcggaacatt gaccgagacg atggacgtcg tccgggcggc cgccgagggc
     5341 gggttcggcc gggtcatgtc gcaccgctcc ggcgagacgg aggatgcgac gatcgcggac
     5401 ctcgcggtcg ccgggggcac gggacagatc aagacgggcg ctccctcgcg cggggagcgt
     5461 accgcgaagt acaaccagct gctccggatc gaagaggagc tcggcgaggg cgcccgctac
     5521 gccgggacag cgccgttccg gaggtacgcc cggtgagcag ccgagccgag gttgggacgc
     5581 ggccccggct gcatccgagg agcgcgctcc tcggcgcgct cctcttgctg ttggccgcga
     5641 cggggatcgt gccggtcaag cagtacctcg cccagcgtga gcggatggcc gacctcgagc
     5701 gcaaggtcga gctgctcgca accgagcggg agcgcctccg cgcgcgcatc gcccagctcc
     5761 aggacccggc ggagctcgag cgcctcgccc gcgagtgcct ggggatggtc aagcccggcg
     5821 agatcgcctt cgtgggcgtc cccgagggcg gaggcccagc cgccgactgc tgaaacagac
     5881 ggcctcggca gggccgccca gacacccgca acggaccatt gacgcctcgc cggggcgggt
     5941 gggatagtga ccttcctgtt cgcccggggg cttccggccc cccacgagga aggagtcgtg
     6001 atcgcagatc tcgaagtcgg cgcggtcgtg gaggggaccg tgcagcgcat cacgccctac
     6061 ggggcgttcc tccagctcga ggacgggcgg gtcggtctcg tccacatctc cgagatcgac
     6121 cgtaactacg tgaaggacgt ccgcgagcac ctgcgcgaga acgacaaggt gcaggcgaag
     6181 gtccagtcga tcaaggagga cggcaagatc gacctgtcga tcaaggccct ccaggacccg
     6241 ccgagacgac cgctgcggcg cggctccgac ccggacctcg agcacaagct caagcgcttc
     6301 atgaagcaga gcgaggagcg gcaggtcgac ttccgccgct ccatggacaa gcgccggtag
     6361 ctccgatcga gcttcgcgag cgcgacctcg ctgccgtcac cgagcagttg gggcgtcctc
     6421 ccatgtccag gttcggggtc gtggcccgct gtggggtcgg acacccactg gtcatccgca
     6481 atcacccgtt ggaccgtgag ggtcggccgt tccccacgct gttctggctg acctgtcccg
     6541 acgcggtccg cgccgtttcg cgactggagg cggatgggtg gatcaagcgt ttagcggagc
     6601 gagccgagca ggagccggcc ttcggacagg agctggcgga ggcgcacgag cggcacgcgc
     6661 gcgagcgtgg gcggctcgcg agcgagggtg aaggctgggg cggcgtcgcc gggagcccag
     6721 gcggggtgaa gtgcctccac gcgcactacg cgaaccacct cgcgggcggc ggcgacccgg
     6781 tgggggcgtg ggtggccgag cgggtcgagc cggtccacgc acgtgggggt g
//
LOCUS       DATLGG010000163         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_5342, whole genome shotgun sequence.
ACCESSION   DATLGG010000163 DATLGG010000000
VERSION     DATLGG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5342"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VM638_04690"
     CDS             <1..768
                     /locus_tag="VM638_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVM11754.1"
                     /translation="SMEELDGRSRAILERRFEDPFTLIRRAIGSAYVAGRSFVRVPAP
                     MLRAPAVLQRAFPGGLFANAEGKRRFAEELPVGWPERDLWLCTYDIHRGRRVVLGRPS
                     RRPTKGPLASLQQAVLASCAIPGVYPPTQVGSLTLVDGGVWSTTNLDLAAKAGCDLII
                     GVAPMAYDTVGPLPNPLMQLVRRVPARMLVNEMADARERGAEVLLIRPSAGELRIHGS
                     NLMRRAGWERVARAAYESTCDLLQTERFQPLLAAKAA"
     gene            820..1041
                     /locus_tag="VM638_04695"
     CDS             820..1041
                     /locus_tag="VM638_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11755.1"
                     /translation="MGLTRETVGTPLNMERHDQRTELWASDPVTGESKLIEVFDHPLS
                     EEDLRAFAVAASLIYIDDLTVEDLWSLGA"
     gene            1083..1994
                     /locus_tag="VM638_04700"
     CDS             1083..1994
                     /locus_tag="VM638_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVM11756.1"
                     /translation="MEDRFERLTNLVTFLLDQRNGASFAQILEEIPGWPEGEDARRRA
                     FERDKRVLRDEGIPLIEEKGLYRIPPERFYLPDLELTEDEQVALRLAVAAVPGSSELG
                     ANALNKLTLGTGAGGDEPVPVVAALDEQPILPILHAAIRDRATLRFRYAGGDEVREVE
                     PALLFFREGNWYVVGHDRLRDARRSFRVDRIDGDVEVGPAGSFEPAATTAEEAMPRQA
                     WLYGDAAEPEQATVEVDAVLAAKAIADIGGRGTVERRADGSAVITMPVTNREAFRTWV
                     LGMLDHAVVLGSPELRADVIAWLESIR"
     gene            1991..>2881
                     /locus_tag="VM638_04705"
     CDS             1991..>2881
                     /locus_tag="VM638_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVM11757.1"
                     /translation="MSGRPKTDLGARLRRVLLMIPWLLEEGGSTVAEIARRFETTEKE
                     VIRDLTLVMCCGLPPYGGGDLITIVLDDDGTVEAWPGPFFTRPMQLTPREGFAVLAAG
                     RALEAVPGTAQRGALASALAKLEAALGASGTLAVDLEAPPHLATVQRAATAGERLQVT
                     YYSAWRDDVTERVVEPLVVHSRDGRWYAETRDVTDPAAGPRERRLRVDRIRSAEPTGE
                     TFEVPPTPPAPPERLYVPGPDAVEVTVLLPPTASWFADWMETESVEELDDGRLRVRLF
                     VEGEKVLERLLLRVGPEAVVE"
BASE COUNT          405 a         1010 c         1051 g          415 t
ORIGIN      
        1 tcgatggagg agctcgacgg ccgcagccgg gccattctcg agcgccggtt cgaagacccc
       61 ttcaccctga tccggcgggc gatcgggtcc gcgtacgtgg ccggccgctc gttcgtccgg
      121 gtgccggcgc cgatgctgcg cgctcctgcg gtcctgcaga gggccttccc cggagggctg
      181 ttcgcgaacg cggaggggaa gcgtcggttc gccgaggagc ttccggtcgg gtggcccgag
      241 cgcgacctct ggctctgcac gtacgacatc caccgcgggc gtcgagtcgt cctcggccgg
      301 cccagccgcc gccccaccaa ggggccgctg gcgagcctcc agcaggcggt gctcgcgtcg
      361 tgcgcgatcc cgggcgtgta tccgcccacg caggtcgggt cgctcaccct ggtcgacggc
      421 ggcgtgtggt cgacgacgaa cctcgatctc gcggcgaagg ccgggtgcga cctgatcatc
      481 ggcgtcgcac cgatggctta cgacacggtc ggcccgctcc cgaacccgct gatgcagctg
      541 gtccgccggg ttccggcccg gatgctggtg aacgagatgg cggacgcccg ggagcgcggt
      601 gccgaggtgc tgctgatccg cccgtccgca ggggagctcc ggatccacgg gtcgaacctg
      661 atgcgccgag cgggttggga acgggtggct cgcgccgcgt acgagagcac ctgcgatctc
      721 ctgcagacgg agcgcttcca gccgctcctc gccgcgaaag cggcctgaag ccgcgccctg
      781 acaccgcagc ctggcgccgt cgagggcacc gtccgttgca tggggttaac ccgcgagacc
      841 gtgggtacac cgctgaacat ggaacggcac gatcagcgga ccgagctctg ggcatcggac
      901 cctgtgacgg gcgaatcgaa gctgatcgag gtcttcgacc atccgctcag cgaggaagac
      961 ctgcgggcct tcgccgtcgc agcgtcgctc atctacatcg acgacctcac cgtggaagac
     1021 ctctggtctc tcggcgcctg agcgtctgag gcggggaacc gcctggcaga ctcggtcctc
     1081 gcatggagga tcgattcgaa cgactgacca acctggtcac cttcctcctc gaccagcgga
     1141 acggcgcctc gttcgcgcag atcctcgagg agatcccggg ctggcccgag ggcgaagacg
     1201 cccgccgccg agccttcgag cgtgacaagc gggtgctccg cgacgagggt atccccctca
     1261 tcgaggagaa gggcctctac cggatccctc ctgagcggtt ctacctgccc gatctcgaac
     1321 tgacggagga cgagcaggtc gccctgcggc tggcggtggc cgcggtgccc ggcagcagcg
     1381 agctgggcgc gaacgcgctc aacaagctga ccctcgggac gggagccggc ggcgacgagc
     1441 cggtgccggt cgtcgccgcg ctcgacgagc aaccgatcct cccgatcctc cacgccgcca
     1501 tccgcgaccg ggcaaccctc cggttccgct acgccggtgg agacgaggtt cgtgaggtcg
     1561 aaccggcgct gctcttcttc cgcgaaggca actggtacgt ggtgggccac gaccggttgc
     1621 gggacgcccg ccgcagcttc cgggtggacc gcatcgacgg cgacgtcgag gtcggcccgg
     1681 cgggatcgtt cgaacctgca gccaccaccg ccgaggaggc gatgccccgc caggcctggc
     1741 tctacggcga tgccgccgag ccggagcagg cgacggtgga ggtcgacgcc gtgctcgcag
     1801 ccaaggcgat cgccgacatc ggcggccggg gcaccgtcga gcgccgggcg gacggatccg
     1861 cggtgatcac gatgcccgtc acgaatcgcg aggcgtttcg cacctgggtg ctcgggatgc
     1921 tcgaccacgc ggtggtgctc ggctcgccgg agctgcgggc cgacgtgatc gcgtggctgg
     1981 agtcgatccg atgagcgggc gaccgaagac cgacctcggg gcgcgcctgc ggcgggtgct
     2041 gctgatgatc ccgtggctcc tcgaggaggg ggggtcgacg gtggccgaga tcgcccgccg
     2101 cttcgagacg acggagaagg aggtgattcg cgacctcacg ctcgtcatgt gctgcggcct
     2161 gcctccgtac ggcggcggcg acctgatcac catcgttctc gacgacgacg gaacggtcga
     2221 agcatggccc ggcccgttct tcacccggcc gatgcaactc acgccgcggg agggcttcgc
     2281 ggtgctcgcc gccggccgcg cgctcgaggc ggtgccgggg accgcccagc ggggggcgct
     2341 cgcttccgcg ctggcgaagc tcgaggccgc cctcggtgcg tcgggaacac tcgcggtcga
     2401 cctcgaggcg ccgccccacc tcgccacggt gcagcgggcg gccacggccg gcgagcggct
     2461 gcaggtcacc tactactccg cgtggcggga cgacgtcacc gagcgggtgg tggaacccct
     2521 cgtcgtccat tcccgggacg gacgctggta cgcggagacg cgcgacgtca ccgatccggc
     2581 ggccggtcct cgcgagcgcc ggcttcgggt cgatcggatc cgctccgccg agccgacggg
     2641 ggagaccttc gaggtgccgc ccacgccgcc ggcacctccc gagcggctgt acgtgccggg
     2701 gcctgatgcg gtcgaggtca ccgtgctgct ccctccgacc gcctcgtggt tcgccgactg
     2761 gatggagacg gagtcggtcg aggagctcga cgacgggcga ctgcgggtcc ggctgttcgt
     2821 cgaaggcgag aaggtgctcg agcgcctgct gctccgcgtc ggccccgaag cggtcgtcga
     2881 g
//
LOCUS       DATLGG010000164         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_26915, whole genome shotgun sequence.
ACCESSION   DATLGG010000164 DATLGG010000000
VERSION     DATLGG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26915"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="VM638_04710"
     CDS             complement(<1..2486)
                     /locus_tag="VM638_04710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HVM11758.1"
                     /translation="MATAKSDTQAPEPAVEEVVNTVVDEGASLGVRRYFTIPGRDPFD
                     EIEWEIRDAFIPGKDKPAFEQKGVEFPKFWSQTATNIVAQKYFRGRLSSPERESSVKQ
                     MLGRVVGTIGAWGRDGGYFASEEEAETFEAELKAILVNQLASFNSPVWFNVGFEEKPQ
                     CSACFILSIDDSMESILDWIRREGVIFRGGSGSGVNLSRLRSSKEQLSKGGYASGPVS
                     FMRGADASAGTIKSGGKTRRAAKMVVLDVDHPDVEEFIWCKAKEERKARVLEQAGYDM
                     SLDSPDWASIQYQNANNSVRVSDAFMEAVVEGKEWNLTARTDGTVVDTVDARELLRQI
                     AAAAWECADPGVQYDTTINAWHTLPNTGRINASNPCSEYMSIDDSACNLASLNLMKFR
                     REDGELDVEAFTHAVDTVFLAQEILVGYSSYPTPEIERNAKAYRQLGLGYANLGALLM
                     SRGLPYDSDEGRAYAAAVTALMTGRAYRKSAEIAARMGAFAGYRPNAAAMIGVIAKHR
                     AAVGNIEDSHSVPDDLLRAARQAWDDALDLGEVSGYRNAQATVLAPTGTISFMMDCDT
                     TGVEPDFSLVKSKKLVGGGELTIVNRSVPMALEKLGYAPPEVEEVVAFVDDRNTVVGA
                     PYVKTEHYPVFDCAIGERAIHYMGHVKMMGAVQPFISGAISKTVNLPETATVEDVSQL
                     LVESWQLGVKAIAIYRDNCKVAQPLSGKSDTAAAAPLAAGAVVAASPQRRRLPDDRTE
                     VGRKFRVGDYEGYIHVGLFEDGTPGDIFVDIAKEGTTLAGLMNSFMISVSLGLQYGVP
                     LEVYVSKFSHMRFEPSGPTNDPDIRIAKSIV"
     gene            complement(2655..>2901)
                     /locus_tag="VM638_04715"
     CDS             complement(2655..>2901)
                     /locus_tag="VM638_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11759.1"
                     /translation="SGGDDDPDDGDATAAPNDEDDGFIGFFRPSNADEIPLPLLVLAG
                     LALALLAAAAAGFVARRVQARRLRPAGIDDRPAPPDA"
BASE COUNT          415 a         1024 c          994 g          468 t
ORIGIN      
        1 acgatcgact tcgcgatccg gatgtccggg tcgttcgtcg ggcccgacgg ctcgaagcgc
       61 atgtggctga acttcgagac gtagacctcc agcggcacgc cgtactggag gccgagcgac
      121 accgagatca tgaacgagtt catcaggccg gcgagggtcg tcccctcctt cgcgatgtcg
      181 acgaagatgt cgcccggggt gccgtcctcg aacaggccga cgtggatgta cccctcgtag
      241 tcgccgacgc ggaacttgcg gccgacctcg gtccggtcgt ccggcaggcg ccggcgctgg
      301 ggcgacgcgg cgacgacggc gccggccgcg agcggggcgg ccgcggcggt gtcgctcttt
      361 ccggacagcg gctgcgcgac cttgcagttg tcgcggtaga tcgcgatcgc cttcacgccg
      421 agctgccacg actcgacgag gagctgcgag acgtcctcga ccgtcgcggt ctccggcagg
      481 ttgaccgtct tggagatcgc gcccgagatg aacggctgga cggcgcccat catcttcacg
      541 tgacccatgt agtggatcgc gcgctcgccg atcgcgcagt cgaagacggg gtagtgctcc
      601 gtcttgacgt acggcgcgcc gacgacggtg ttgcggtcgt cgacgaacgc gacgacctcc
      661 tcaacctccg gcggcgcgta gccgagcttc tcgagggcca tcggcacgga gcggttcacg
      721 atcgtgagct ccccgccgcc gacgagcttc ttcgacttca cgagcgagaa atccggctcc
      781 acgcccgtcg tatcgcagtc catcatgaag ctgatcgtcc cggtgggcgc gagcacggtc
      841 gcctgcgcgt tgcggtagcc gctgacctcg ccgaggtcga gcgcgtcgtc ccacgcctgc
      901 cgcgccgcgc ggagcaggtc gtccggcacc gagtgcgagt cctcgatgtt gccgacggcc
      961 gcgcggtgct tcgcgatcac gccgatcatc gccgccgcgt tcggccggta gcccgcgaac
     1021 gcgcccatcc gcgccgcgat ctcggccgac ttccggtacg cgcgccccgt catcagcgcc
     1081 gtcaccgcag ccgcgtacgc gcggccctcg tcggagtcgt acggaagccc gcgcgacatc
     1141 agcagcgcgc cgaggttcgc gtagccgagc ccgagctgcc ggtacgcctt cgcgttccgc
     1201 tcgatctcgg gcgtcgggta cgacgagtag ccgaccagga tctcctgcgc gaggaacacc
     1261 gtgtcgaccg cgtgcgtgaa cgcctcgacg tcgagctccc cgtcctcgcg gcggaacttc
     1321 atcaggttca gcgaggcgag gttgcacgcg gagtcgtcga tcgacatgta ctccgagcac
     1381 gggttcgacg cgttgatccg ccccgtgttc gggagcgtgt gccacgcgtt gatcgtcgtg
     1441 tcgtactgca cgccggggtc ggcgcactcc cacgcggcgg cggcgatctg ccggagcagc
     1501 tcgcgcgcgt cgacggtgtc gacgaccgtg ccgtcggtgc gcgccgtcag gttccactcc
     1561 ttgccctcga cgaccgcctc catgaaggcg tcgctgacgc ggacggagtt gttcgcgttc
     1621 tggtactgga tcgacgccca gtccggcgag tcgagcgaca tgtcgtagcc cgcctgctcg
     1681 aggacgcgcg ccttccgctc ctccttggcc ttgcaccaga tgaactcctc gacgtccggg
     1741 tggtcgacgt ccaggacgac catcttcgcg gcgcgacgcg tcttcccgcc cgacttgatc
     1801 gtccccgcgg acgcgtcggc gccgcgcatg aacgagacgg gcccggaggc gtagccgccc
     1861 ttcgagagct gctccttcga cgagcgcagg cgcgagaggt tgacccccga gccggagccg
     1921 ccgcggaaga tgacgccctc ccggcggatc cagtcgagga tcgactccat cgagtcgtcg
     1981 atcgagagga tgaagcaggc ggagcactgc ggcttctcct cgaagccgac gttgaaccag
     2041 accggcgagt tgaacgaggc gagctggttg acgaggatcg ccttcagctc ggcctcgaac
     2101 gtctccgcct cctcctcgga ggcgaagtag ccgccgtcgc ggccccacgc gccgatcgtc
     2161 cccacgacgc gcccgagcat ctgcttcacg ctcgactcgc gctcgggcga gctcaggcgg
     2221 ccgcggaagt acttctgcgc gacgatgttg gtcgccgtct gcgaccagaa cttcgggaac
     2281 tcgacgccct tctgctcgaa cgccggcttg tccttgccgg ggatgaacgc gtcgcggatc
     2341 tcccactcga tctcgtcgaa cgggtcgcgg cccggaatgg tgaagtagcg gcggacgccc
     2401 aggctggccc cctcgtcgac gacggtgttc accacctcct cgacggccgg ctccggtgcc
     2461 tgtgtgtcgc tcttcgcggt cgccatctct acctcccggg tacgcctgct cgaaagactc
     2521 gattcgatcg acctgcggac cccccgaaag cggtgtcggt cgaccggact cggaacccta
     2581 gagagaggct cggacggatc ccggaggcct ccgaattgta cccggtgcgg cccttccggc
     2641 cggctccggg gggcctacgc gtcgggcggc gcgggacggt cgtcgatccc ggccgggcgg
     2701 agcctccggg cctgcacgcg gcgtgcgacg aagccggccg cggcggccgc gaggagcgcg
     2761 agcgcgagcc ctgcgaggac gagcagggga agcgggatct cgtccgcgtt gctcgggcgg
     2821 aagaagccga tgaagccgtc gtcctcgtcg ttcggcgccg ccgtggcgtc gccgtcgtcg
     2881 ggatcatcgt ctccgccgga a
//
LOCUS       DATLGG010000165         3774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_43025, whole genome shotgun sequence.
ACCESSION   DATLGG010000165 DATLGG010000000
VERSION     DATLGG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43025"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VM638_04720"
     CDS             complement(<1..527)
                     /locus_tag="VM638_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11760.1"
                     /translation="MSLLLLTVITVLAGGSVHAASPQITDPEGDANGLLYSAGPDTRP
                     VSYDPADLVSVRLQTAYRTIRVGDDGLRHEPTALQIRFTTLAPPQAPGTSLGFLLHAG
                     TGSQTSLIKGFLGGDVPDPRAGDVEWTLVTERAYVLGTGEVTKDRAWFGTAVPGGSDF
                     VITIPFASLTPAQLR"
     gene            complement(663..1862)
                     /locus_tag="VM638_04725"
     CDS             complement(663..1862)
                     /locus_tag="VM638_04725"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVM11761.1"
                     /translation="MADTTEERSAYSQLGEQWLLRGGGLDIITTEERDLSTEEMVLNI
                     GPQHPSTHGVLRVVLEMEGEMITRAEPVIGYMHRGAEKMAEHRDHRQTLVLMNRHDWL
                     SAFNNELGWVLAVEKLAGIEVPDRAQWIRTMLAELNRLLNHWMFCGSFPLELGAMTPM
                     FYAFREREMIQSLMEAVTGGRIHFMYSRVGGLKDDLPAGFLKEAAQVVRWNRKKLKDF
                     ERLVMGNEIIHARTKGVGILPPDVAKGFGASGPVLQASGVAMDPRKDEPYEKYAEVDF
                     DVPVGENGDSYDRLWVLVQRMWQSCDIIEQCLERLPSGPFIQPKLPKKINIPEGEVYV
                     RTENPLGLMAYYLVGYGKKYPYRLKMRTASFSNVAVLPWILPGKLLPDLIAILGSIFF
                     VVGDVDK"
     gene            1968..2723
                     /locus_tag="VM638_04730"
     CDS             1968..2723
                     /locus_tag="VM638_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11762.1"
                     /translation="MKKLGTVLAVVLALGASAPAGANAPINAAVFAADCADGTVVVDG
                     TMNVFGGVGVIRDGICAVQMTPHSRLFFRDARLLGADVFLVIADAQQHTRVEALRTSI
                     EFTGAESAIQLSPGCCAGDPEEGRDERYGWVEIIDSSLHAATVEVSASYASSHGQTFV
                     KRSRIVATHTDGLASLRITASNTGSHGHVLVEDSDLRSGDAIRIDTFERGRTLALRNR
                     FLSEPALIYTGPRGTCISRHNVPETPCVAHPGD"
     gene            complement(2785..>3774)
                     /locus_tag="VM638_04735"
     CDS             complement(2785..>3774)
                     /locus_tag="VM638_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11763.1"
                     /translation="IKNCRVGQGDIQVAKDGTVAAIASQTSGSCQTHDGRNVPRGSAL
                     VDMRDVYDPLVVGSAPEPGGSHNQTIHPSGDYLYISISSGSGKIPIFDIRDPENPRKV
                     RDWNPGSGNNPHDIRFSDDGKRAYMAGIAQFRIVNTENPESPVLISSFTPPGSTIGHD
                     VLVTPDKRFLFAGDELNGGSTAPCPGGAIYTYDIGDEANPRLIGIAEAGGGPVIGFQA
                     DTPYPTGGTGGCTSHVMDMNPDKKSLSIGWYVLGTRTFSWASLYNADGTPKNVQGVTF
                     TWGSRATGDGLVETGYIIPQGANTWAAKQYHRVPGYIFSDDLNLGFYITKVKS"
BASE COUNT          567 a         1261 c         1339 g          607 t
ORIGIN      
        1 aagcggagct gagccggggt cagcgacgcg aacgggatcg tgatgacgaa gtcgctcccg
       61 ccgggaacgg cggtcccgaa ccaggcgcgg tccttcgtca cctcgccggt cccgaggacg
      121 taggcccgct ccgtcacgag ggtccactcg acgtccccgg cccgcgggtc cggaacgtcc
      181 ccgcccagga agcccttgat gagggaggtc tggctgccgg tcccggcgtg cagcaggaag
      241 ccgagggagg tgccaggcgc ctgcggcgga gccagcgtcg tgaaccggat ctggagggcg
      301 gtgggctcgt ggcggaggcc gtcgtccccg acgcggatgg tgcggtaggc ggtctggagc
      361 cggacggaca cgaggtccgc cgggtcgtag gagacggggc gcgtgtcggg gccggcggaa
      421 tacaggaggc cgttcgcgtc cccctcggga tccgtgatct gcggtgacgc ggcgtgcacg
      481 cttccgccgg cgagcacggt gatgacggtc agcagcaaca gcgacatccg gcgcatcctc
      541 cgcgatcctc ctcgtctcgt cgtcacgaac cacctgcatc ttcgcgcatg ggacacgaga
      601 tccctttggg cagatgcaca gtctttcggc ggatggcggc ggaaccgccg tggcgtggcg
      661 gtctacttgt cgacgtcgcc gacgacgaag aagatcgagc cgaggatcgc gatcaggtcg
      721 ggcaggagct tcccgggcag gatccacggc agcacggcga cgttggagaa cgaggccgtc
      781 cgcatcttca gccggtaggg gtacttcttg ccgtacccga cgaggtagta cgccatgagc
      841 ccgagcgggt tctcggtccg cacgtagacc tcgccctcgg ggatgttgat cttcttcggg
      901 agcttcggct ggatgaaggg ccccgacggg aggcgctcga ggcactgttc gatgatgtcg
      961 cacgactgcc acatccgctg gacgaggacc cacagccggt cgtaggagtc gccgttctcg
     1021 ccgaccggga cgtcgaagtc cacctcggcg tacttctcgt acggctcgtc cttccgcggg
     1081 tccatcgcca cgccggatgc ctgcaggacc gggcccgagg caccgaagcc cttcgccacg
     1141 tccggcggca ggatgcccac gcccttcgtg cgggcgtgga tgatctcgtt gcccatcacc
     1201 agccgttcga agtccttcag cttcttgcgg ttccagcgca cgacctgcgc ggcctccttc
     1261 aggaacccgg ccggcaggtc gtccttcaga ccgccgacgc gcgagtacat gaagtggatc
     1321 cggcccccgg tgacggcttc catcagcgac tggatcatct cgcgctcgcg gaaggcgtag
     1381 aacatcggcg tcatcgcgcc gagctcgagc gggaaggagc cgcagaacat ccagtggttc
     1441 agcaggcggt tgagctcggc gagcatcgtg cggatccact gtgcccgatc gggaacctcg
     1501 atccccgcga gcttctcgac cgcgagcacc cagcccagct cgttgttgaa cgccgacagc
     1561 cagtcgtggc ggttcatgag cacgagcgtc tggcggtggt cgcggtgctc ggccatcttc
     1621 tccgcgcccc ggtgcatgta cccgatgacg ggctcggcgc gggtgatcat ctcgccctcc
     1681 atctcgagga cgacccgcag gacgccgtgg gtggaggggt gctgggggcc gatgttgagg
     1741 accatctcct cggtggagag gtcgcgctcc tcggtcgtga tgatgtcgag gccgcccccg
     1801 cgcagcagcc actgctcgcc gagctgggag tacgccgagc gctcttcggt ggtgtccgcc
     1861 atgccgccgc ctagcctagc ggacccgccg gggagacgcc gctcccggct tgccccggaa
     1921 cgggccatgc cgcagagtgc gcgggtccga cagaagggag gaatgggatg aagaagctcg
     1981 gaaccgtgct ggccgtcgtg ctggcgctcg gcgcctcggc ccccgcggga gcgaacgcgc
     2041 cgatcaacgc ggccgtgttc gcggccgact gcgccgacgg aacggtcgtc gtggacggga
     2101 cgatgaacgt cttcggcggt gtgggggtga tccgcgacgg gatctgcgcc gtccagatga
     2161 cgccgcacag ccggttgttc ttccgcgatg cgcgcctgct gggagcggac gtcttcctgg
     2221 tgatcgccga cgcgcagcag cacacgcggg tcgaggctct ccggacgtcg atcgagttca
     2281 ccggcgccga gagcgcgatc cagctctccc cgggctgctg cgcgggcgac cccgaggagg
     2341 ggcgcgacga gcgatacggg tgggtcgaga tcatcgacag cagcctgcac gcggccacgg
     2401 tcgaagtgtc cgcatcatac gcatcgagcc acgggcagac gttcgtgaag cgctccagga
     2461 tcgtagcaac gcacaccgat ggtctcgcat ccctgcggat cacggcatcg aacacaggct
     2521 cgcacggaca cgtcctcgtc gaggactccg acctgcgcag cggcgacgcg atccgcatcg
     2581 acacgttcga gcggggtcgc acgctggcgc tgcgcaaccg cttcctgagc gagccggcgt
     2641 tgatctacac ggggccgcgc gggacctgca tctcgcgcca caacgtcccc gagacgccct
     2701 gcgtcgctca ccccggcgac tgacggcggg aacgcgaagg gggcggccga ccggccgccc
     2761 cctcatcgcg tgcggaaccc gcggttacga cttgaccttg gtgatgtaga agcccaggtt
     2821 caggtcgtcg gagaagatgt agcccggaac gcggtggtac tgcttcgcgg cccaggtgtt
     2881 cgcgccctgc gggatgatgt agccggtctc gacgagaccg tccccggtcg ccctcgaccc
     2941 ccaggtgaac gtcacgccct ggacgttctt cggcgtcccg tcggcgttgt agagcgacgc
     3001 ccagctgaac gtccgggtcc ccaggacgta ccacccgatc gacagcgact tcttgtcggg
     3061 gttcatgtcc atcacgtgcg aggtgcatcc gcccgtgccg cccgtcgggt acggggtgtc
     3121 ggcctggaag ccgatcaccg ggccaccgcc ggcctcggcg atgccgatca gccgcgggtt
     3181 cgcctcgtcg ccgatgtcgt aggtgtagat cgcgccgccc gggcagggag cggtcgaccc
     3241 cccgttcagc tcatcgccgg cgaagaggaa ccgcttgtcc ggcgtgacga gcacgtcgtg
     3301 gccgatcgtg ctgcccggcg gggtgaacga cgagatgagg accgggctct ccgggttctc
     3361 cgtgttcacg atccggaact gcgcgatccc ggccatgtac gcgcgcttcc cgtcgtcgct
     3421 gaaccggatg tcgtgcgggt tgttgcccga gcccgggttc cagtcgcgga ccttgcgggg
     3481 gttctcgggg tcgcggatgt cgaagatcgg gatcttcccg gagccggacg agatcgagat
     3541 gtagaggtag tcgccgctcg ggtggatcgt ctggttgtgc gagccacccg gctccggcgc
     3601 gctgccgacc acgagcgggt cgtagacgtc gcgcatgtcg acgagcgccg agccgcgcgg
     3661 gacgttgcgg ccgtcgtgcg tctggcagga gccgctcgtc tgcgaggcga tcgcggcgac
     3721 cgtgccgtcc ttcgccacct ggatgtcgcc ctgaccgacg cggcagttct tgat
//
LOCUS       DATLGG010000166         6229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_70050, whole genome shotgun sequence.
ACCESSION   DATLGG010000166 DATLGG010000000
VERSION     DATLGG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70050"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..980
                     /locus_tag="VM638_04740"
     CDS             162..980
                     /locus_tag="VM638_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11764.1"
                     /translation="MDPSGLAVLSGWSVIEAHGADARDWLHDLLTADVESLATGRSAV
                     ALLLTPTGRIRASAVVVATEDAILLVQGPGQPRPIADLLEPYVLSSAVELVPTRLVPV
                     ARLGAGGLSGRYGHAPSALGAGAGTFLSGDDVDALPSDDLIAPAELERWRVERGVARF
                     PVDLTPESLPHEADFGDAIDLEKGCYLGQEAVARVRNLGHPTRLVLAAEVEGGANAGD
                     AVEGPDGPVGEVTSVAGQGSDAVLIRVRWAAREAPLRLSDGRALRMLGRARGGA"
     gene            complement(945..1868)
                     /gene="sepH"
                     /locus_tag="VM638_04745"
     CDS             complement(945..1868)
                     /gene="sepH"
                     /locus_tag="VM638_04745"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HVM11765.1"
                     /translation="MERIHLVGRSADGERLVFARGGRDRTLFEVPITPELRTLVAPER
                     GDDRGVAAAGDAGPPPTSHEHEDGHVRLFPTAPEAPPPRRRHGGLAPGALARATGLAT
                     PREGVGPRGVHALIGDGGTALAPPTPEPEPAREPERSADDPAGDAVIHTIRPRLTAGQ
                     IQAKLRAGRTIDEVTRLSGAPQDWVERLDATVQRERHVVVAQMLHEAARHPALGRAPV
                     ALGESLAAHLEHTGSSADALKEGWFAARPEEGGPWRVAWMLADGGVERRAEWRYDPRA
                     RLLEAADDLATELAWPPEERSGAAARPAEHP"
     gene            complement(1942..2109)
                     /locus_tag="VM638_04750"
     CDS             complement(1942..2109)
                     /locus_tag="VM638_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11766.1"
                     /translation="MQGTDRCGDCVVTFILDQPDEAVVVDAEEARALRVLGEAGLVPG
                     LRYEPRREEAG"
     gene            complement(2203..4713)
                     /locus_tag="VM638_04755"
     CDS             complement(2203..4713)
                     /locus_tag="VM638_04755"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HVM11767.1"
                     /translation="MRLRPLIPALLLALALPTPTSIPGDASATAHAGSASFIDDATLQ
                     LLGFRGGSHPAEPSGDWLLRQRAGEDGAIPRDAYARALDQAREIGAMTERAAPEVASA
                     TWEAMGPTNIGGRVTGLAVDPTRGTVAVNAGGARTVVNSHVYAATASGGVWRSSDAGA
                     TWAYRWNRDLTQSIGAIAIGSDGWIHVGTGETNPGGGSVTWGGTGMYRSKDGGATWQQ
                     TGLPTSGRIAHVVVDPTDPKRVFVAASGNLFVPGGERGIYRSLDGGDTWTRVLAGDNP
                     TTGGSFLAIDPKDPNNVLAGMWDHRRFPTHRRYAGPGSGVYRSTDGGSTWARVTLPGA
                     PAPEQVGRIGVAFAPSDPSRAYAIIANNLAGNGVGLWRSNDGGATWTKTAAAVDGTGA
                     TGVNGLSQSSYGWWFGKLWVDPLRPDRVFVAGVELVESLNGGDTFFPHGNTTAMVVSG
                     ANQAYPHADQHAMIWDPLVPNRVYLGNDGGIYVSEANAQVGTWRAAAVQGWTQHYSVD
                     VSEQNPRRVVSGLQDNMCQRNYLAGEGGHPHTWTKFGLCGDGLQTLVNPTNENVIYGC
                     AQYGNNCSRTGNDGHVSSSLSPLLGALPRVPGDRHGWWVPLVFDPNDPSIMYGGSNRV
                     ARSTSGGTSWTTISPDLTTNPPQQDPNSSYRIYGTITTIAVSKSDGNVLHVGTDDGLL
                     WRTTNRGQSWTKLDDEVDPEDPEDTDDIPDGQWVTSVAIDPSDHRTAYATFSGFREGN
                     ASPLVVRTRNGGTRWDDISGNLPAAPVNDLAILPGDRLVVGTDVGVYLTSDAGATWFR
                     VGSNLPAIPVLDLRYHAATNSLTVSTFGHGIQRVVLPA"
     gene            4849..>6229
                     /locus_tag="VM638_04760"
     CDS             4849..>6229
                     /locus_tag="VM638_04760"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HVM11768.1"
                     /translation="MQTIQRSFDDLGIPLREVTFACVDLETTGGAPPASRITEVGAVL
                     YRGGERVRSFQTLVDPGVPIPRFVTHLTGIDDRSVRGAPTIEQVLPSLAELLRGTVFV
                     AHNAAFDHRFLSHDLERLGYPPLPDPVCTVKLARRVLGPGDAPNLRLDTLAQLFRTVR
                     PTHRALDDAEACAAVLHALLELGGRLGILTLEDLRETMRARSHPHRAKLRLAASVPSA
                     PGVYVFRRAATSGGRGEVLYVGKSKDLRARVRSYFGGDPRRKVEALLAELGEVEAIPC
                     ATEVEALVLEARLIRRHAPRYNRRGRSWQRFAYVKLDLEEPWPRLKVVRRIGGRGPIL
                     GPMPPSRARLVKEALEEAFPIRRCTERMGRETRFTPCALADLGRCAAPCDGRVSPDRY
                     EELVRSLHHALSTPGGLLAALEARMARLAEAERFEEAALLRDRIAALVGALTRDRTDR
                     WLVGAGRLVV"
BASE COUNT          766 a         2350 c         2213 g          900 t
ORIGIN      
        1 ctcgccacga cgccgacgac gaacccgagg atcgccagca cgagcacgac ctccaccacg
       61 gtcacgccct cctcgccgat gctccgtcgc acgcccgtcc tcctccggtt ccagcaagcg
      121 taccccgagg catccggccc gacgtggata ccatggccgc gatggacccc tccggtctcg
      181 ccgtcctctc gggctggtcc gtcatcgagg cgcacggggc ggacgcgcgg gactggctgc
      241 acgacctgct caccgccgac gtggagtccc tcgcgaccgg ccggtccgcc gtcgccctgc
      301 tcctcactcc gaccgggcgg atccgcgcgt cggccgtcgt cgtggcgacg gaggatgcga
      361 tcctcctcgt gcagggaccc ggccagcctc gtccgatcgc cgacctgctc gagccctacg
      421 tgctctcttc cgcggtcgag ctcgtcccga cccggctcgt cccggtcgcc cgcctcgggg
      481 cgggtggcct ctcgggtcgc tacgggcacg ctccctccgc gctcggcgcg ggcgcgggca
      541 cgttcctcag cggggacgac gtcgacgcgc tgccatccga cgacctcatc gcaccggccg
      601 agctcgagcg atggcgggtc gagcgcggcg tggctcggtt ccccgtcgac ctcacgcccg
      661 agtcgctgcc gcacgaggcc gacttcggcg acgcgatcga cctcgagaaa ggctgctacc
      721 tggggcagga ggcggtcgcg cgcgtccgca acctcggcca tccgacgcgg ctcgtcctcg
      781 ccgcggaggt cgagggcggc gcgaacgccg gtgacgccgt cgagggaccg gacgggccgg
      841 tgggcgaggt gacgagcgtc gccgggcagg ggtcggacgc ggtgctcatc cgggtgcgat
      901 gggcggcgcg agaggcgccg ctgcggctct ctgacggccg ggccctacgg atgctcggcc
      961 gggcgcgcgg cggcgcctga ccgttcctcg ggcggccagg cgagctccgt ggcgaggtcg
     1021 tccgcggcct cgaggagacg cgcccgcggg tcgtagcgcc actcggcgcg ccgctcgacc
     1081 ccaccgtcgg cgagcatcca ggccacgcgc caggggccgc cttcctcggg acgggcggcg
     1141 aaccacccct ccttcaacgc gtcggcggac gagccggtgt gctcgagatg cgccgcgagc
     1201 gactccccga gcgcgaccgg cgcgcgaccc agcgccgggt ggcgcgcggc ctcgtgcagc
     1261 atctgcgcga ccacgacgtg ccgctcgcgc tgcacggtgg cgtcgaggcg ctcgacccag
     1321 tcctgcggag cgccggagag gcgggtgacc tcatcgatcg tccgaccggc ccgcagcttc
     1381 gcctggatct gcccggcggt gaggcgggga cggatcgtgt ggatcaccgc gtcccccgcg
     1441 ggatcatcgg cggagcgctc gggctcgcgc gccggctcgg gctcgggcgt gggcggagcg
     1501 agcgcggtcc cgccgtcacc gatcagcgcg tggacgccgc gcggaccgac gccctcccgg
     1561 ggtgtcgcga gaccggtggc gcgcgccagc gcccccggtg cgaggcctcc gtggcgccgc
     1621 ctcggcgggg gcgcctcggg ggcggtcggg aagagccgca cgtggccgtc ttcgtgctcg
     1681 tggctggtcg gaggcggtcc ggcgtcgccc gccgccgcga cccctcggtc gtcccctcgt
     1741 tccggcgcga cgagcgtccg cagctcgggc gtgatcggaa cctcgaacag cgtccgatcc
     1801 ctgccaccgc gagcgaagac gagccgctcg ccgtcggcgc tccgcccgac caggtggatc
     1861 cgctccatcg ccctccttcc gcgcgctccc gatgaccagt gtcacccgcg gaaggggtcg
     1921 aatcaacgaa ccggcatctc ctcagccggc ctcctcgcgc cgcggctcgt accgcagccc
     1981 cgggacgagt ccggcctctc cgaggacgcg cagggcccgc gcctcctcgg cgtcgacgac
     2041 caccgcctcg tccggctgat cgaggatgaa cgtcacgacg cagtccccgc accggtccgt
     2101 cccctgcatc acgcaatcgt cacagctcca ccgcacggct cctcctctcg gatctgggcc
     2161 gcgcggaacc caccctagcc ggggggtctg acacgaacgc cctcaggccg ggaggacgac
     2221 ccgctggatg ccgtgcccga aggtcgagac cgtgagactg ttcgtcgcgg cgtggtaccg
     2281 caggtcgagc accgggatcg ccggcaggtt ggacccgacc cggaaccacg tcgctcccgc
     2341 gtcggaggtc aggtagacgc cgacgtccgt cccgacgacg agccgatccc ccggcaggat
     2401 cgccagatcg ttcaccgggg cggccgggag gttcccgctg atgtcgtccc accgcgttcc
     2461 gccgttccgg gtccgcacga cgaggggaga ggcgttgccc tcgcggaagc cggagaaggt
     2521 ggcgtacgcc gttcggtggt ccgaaggatc gatcgcgacg gaggtcaccc actgcccgtc
     2581 ggggatgtcg tcggtgtcct cggggtcttc ggggtccacc tcgtcgtcga gcttcgtcca
     2641 gctctggccg cggttggtcg tgcgccagag caggccgtcg tcggtaccga cgtggaggac
     2701 gttcccgtcg ctcttcgaga cggcgatcgt cgtgatcgtc ccgtagatcc ggtagctcga
     2761 gttcggatcc tgctgcggcg ggttcgtcgt gaggtcgggg ctgatcgtcg tccagctcgt
     2821 cccgccgctc gtcgaccggg cgacgcggtt cgaacccccg tacatgatgc tcgggtcgtt
     2881 cgggtcgaag acgaggggca cccaccaccc gtggcggtcg ccgggaacgc gcggcagggc
     2941 gcccaggagc ggtgacaggg aggagctgac gtgcccgtcg ttcccggtcc tcgagcagtt
     3001 gttcccgtac tgggcgcagc cgtagatcac gttctcgttc gtcgggttga cgagcgtctg
     3061 cagcccgtcg ccgcacagcc cgaacttcgt ccaggtgtgc ggatgaccgc cttcgcccgc
     3121 gaggtaattg cgctggcaca tgttgtcctg caacccgctc accacccggc gcgggttctg
     3181 ctcgctcacg tcgacgctgt agtgctgggt ccacccctgc accgccgctg ctcgccaggt
     3241 cccgacctgc gcgttcgctt cggagacgta gatgccgccg tcgttgccca ggtacacgcg
     3301 gttcggcacc aacggatccc agatcatcgc gtgctgatcg gcgtgcgggt aggcctggtt
     3361 ggcccccgag acgaccatcg cggtcgtgtt cccgtgcggg aagaaggtgt cgccgccatt
     3421 cagcgactcg acgagctcga cgcccgcgac gaacacacgg tcgggccgca gcgggtcgac
     3481 ccacagcttc ccgaaccacc agccgtagct cgactgcgag aggccgttga cgccggtcgc
     3541 accggtcccg tccacggccg cggcggtctt cgtccaggtc gcacccccgt cgttcgagcg
     3601 ccagagaccg acgccgttcc ccgcgaggtt gttcgcgatg atcgcgtagg cgcgcgacgg
     3661 atccgacggg gcgaaggcga cgccgatccg gcccacctgc tccggtgccg gggcgccggg
     3721 gagggtcacg cgggcccagg tgcttccgcc gtcggtcgac cggtagacgc cggagcccgg
     3781 tcccgcgtag cgccgatgcg tcgggaaccg acggtgatcc cacatcccgg cgaggacgtt
     3841 gttcgggtcc ttcggatcga tcgcgaggaa cgatccaccc gtggtggggt tgtcgccggc
     3901 gaggacgcgg gtccaggtgt cgccgccgtc gagcgaccgg tagatcccgc gctcgccgcc
     3961 ggggacgaag aggttcccgg aggccgcgac gaagacccgc ttcgggtccg tggggtcgac
     4021 cacgacgtgc gcgatgcgcc ccgacgtcgg cagcccggtc tgctgccacg tcgcgccgcc
     4081 gtccttcgag cggtacatcc cggtcccgcc ccacgtgacg ctcccgcctc cggggttcgt
     4141 ctcgccggtg ccgacgtgga tccagccgtc cgagccgatc gcgatcgcgc cgatcgactg
     4201 cgtcaggtcg cggttccagc ggtaggccca ggtcgcgccg gcgtcgctgc tgcgccagac
     4261 gccaccgctc gcggtggcgg cgtagacgtg gctgttcacg acggtcctcg ctcccccggc
     4321 gttgacggcg accgtgcccc gggtcgggtc gacggcgagg cccgtgacac gtcctccgat
     4381 gttcgtcgga cccatcgcct cccacgtcgc gctcgccacc tccggcgccg cgcgttcggt
     4441 catcgcgccg atctcgcggg cctgatcgag cgcgcgcgcg taggcatccc gagggatcgc
     4501 cccgtcctcg cccgcacgct gacggagcag ccagtccccg ctgggttccg cggggtggct
     4561 cccaccccgg aagccgagga gctggagcgt ggcgtcgtcg atgaacgacg cgctccccgc
     4621 gtgcgcggtc gccgaagcgt ccccggggat ggatgtcggt gtggggagcg cgagcgccag
     4681 caggagcgcg gggatcagcg ggcggagccg cacgggcaga cctccttcgg gtcgtgggcg
     4741 atgtcggtcg tctgtttcgc cgtgcgagcc cccgattcct gccgcggctt gacctcgaac
     4801 acctgttcgg gtaccatcgc cgacgcgcgt ggcccgcgag gaggccgcgt gcagacgatc
     4861 cagcgctcgt tcgacgatct ggggatccct ctccgcgagg tgaccttcgc ctgcgtcgac
     4921 ctcgagacga cgggcggggc cccgccggcc tccaggatca cggaggtcgg agcggtcctg
     4981 taccgcggcg gggagcgtgt ccgttccttc cagacgctcg tggatccggg ggtgccgatc
     5041 ccgcggttcg tcacccacct caccgggatc gacgaccggt cggtccgcgg cgccccgacg
     5101 atcgagcagg tcctcccctc gctcgccgag ctgctgcgag ggaccgtgtt cgtcgcgcac
     5161 aacgccgcgt tcgaccaccg gttcctctcc cacgacctcg agcgcctcgg ctatccaccc
     5221 ctcccggacc cggtctgcac ggtcaagctc gcccgtcgcg tcctgggtcc cggggacgcg
     5281 ccgaacctgc gcctcgacac gctcgcccag ctgttccgca ccgtccgtcc gacccaccga
     5341 gcgctcgacg acgccgaggc ttgtgcggcg gtgctccacg cgctcctcga gctcggaggc
     5401 cggctcggca tcctgaccct cgaggacctc cgggagacga tgcgcgcgcg cagccacccg
     5461 catcgggcga agctgcgtct cgccgccagc gtcccctcgg ctcccggcgt ctacgtgttc
     5521 cgacgggcgg cgacgagcgg cggacgcggt gaggtgctct acgtcgggaa gtcgaaggac
     5581 ctccgcgcgc gcgtccgctc gtacttcgga ggcgaccctc ggcggaaggt cgaggcgctc
     5641 ctcgccgagc tcggggaggt cgaggccatc ccctgcgcga ccgaggtcga ggcgctggtg
     5701 ctcgaagcgc gcctgatccg ccggcacgcg ccgcgctaca accgccgagg gcgctcgtgg
     5761 cagcggttcg cctacgtgaa gctcgacctc gaggagccct ggccgcggct gaaggtcgtc
     5821 cgcaggatcg gagggcgggg cccgatcctg ggcccgatgc cgccctcgcg cgccaggctc
     5881 gtgaaggagg cgctcgagga ggccttcccg atccgccgct gcaccgagcg gatgggtcga
     5941 gagacccgct tcaccccctg cgccctcgcg gacctcggcc gctgcgccgc cccctgcgac
     6001 ggccgcgtca gccccgatcg ctacgaggag ctcgtccggt ccctccacca cgccctctcc
     6061 acaccgggcg ggctcctcgc agcgctcgag gcgaggatgg cgaggctcgc cgaggccgag
     6121 cggttcgagg aagcggcgct cctgcgcgac cggatcgccg ccctcgtcgg ggccctcacc
     6181 cgggaccgca cggaccgctg gctcgtcggc gcgggccggc tcgtcgtcg
//
LOCUS       DATLGG010000167         4954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_89755, whole genome shotgun sequence.
ACCESSION   DATLGG010000167 DATLGG010000000
VERSION     DATLGG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89755"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(133..261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(290..1219)
                     /locus_tag="VM638_04765"
     CDS             complement(290..1219)
                     /locus_tag="VM638_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM11769.1"
                     /translation="MAEKILVVDDDPDIARFVELNLRTHGYETHVASDGEEALERAFA
                     VGPDLVLLDVTMPKMDGFEVAQRLRSDPRTSNVSIIMLTARALSVDKVLGLTAGADDY
                     VIKPCDPVELVARVKGTLRRAQEMRALSPLTGLPGNMRIQDELHRLVMEGQPFALLYL
                     DLDNFKAYNDHYGFLRGDEAIRFAARCIQEHALRVVGQEAFIGHVGGDDFVVVCSPDR
                     AEELCRAIIAACDEKIPGLYDPEDRERGHIEVENRRGQVERYPFVSISIGVATTDRRR
                     FSHHGEAVAVATELKEFAKREQGSSFAVDRRGS"
     gene            complement(1220..1879)
                     /locus_tag="VM638_04770"
     CDS             complement(1220..1879)
                     /locus_tag="VM638_04770"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVM11770.1"
                     /translation="MARLAASILSANFARLEDEVKRVEPYADLIHVDVMDAHFVPPLT
                     IGPVVVEWLRPVSDLPLHCHLMVEHPERLFADFAEAGTDLVTFHLEAVEDPGKVAAAA
                     RAAGMAPGIGINPETDIRAAFPHLEGLDNVIVMTLESTGYAGQPFQESSLRKVEALRS
                     EIDRRGLRVDVIVDGGINEATGARCIEAGATMLAAASSIFRADDPARAAEGLARVAAG
                     G"
     gene            complement(1893..3155)
                     /gene="rsmB"
                     /locus_tag="VM638_04775"
                     /pseudo
     CDS             complement(1893..3155)
                     /gene="rsmB"
                     /locus_tag="VM638_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
     gene            complement(3166..4089)
                     /gene="fmt"
                     /locus_tag="VM638_04780"
     CDS             complement(3166..4089)
                     /gene="fmt"
                     /locus_tag="VM638_04780"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVM11771.1"
                     /translation="MRVAFLGSDAWSVPSLEALAGSDHHVAIVGTRPPRPAGRGGKLR
                     PTAVADAARRLGLPLVEPGTVRTGEGFEALRAAGAEALVVVAYGEILPRHVLDLAYPL
                     NVHFSLLPSLRGADPVRRAILSGATTTGVTIMRMDEGMDTGPLLISAEEPIREDDDAG
                     TLGDRLARRGADLLLEALDLLGRGQANERPQDGATATLAPKISREEEWLDWREEAPAL
                     VRRVRALAPDPGARTRYRGKVLKVLRAAAVDGSGEPGAIMEVSNDGIRVAAARGAVAL
                     LEVVPEGRRRISGGDFVRGYRPAPGEVLGAD"
     gene            complement(4090..4614)
                     /gene="def"
                     /locus_tag="VM638_04785"
     CDS             complement(4090..4614)
                     /gene="def"
                     /locus_tag="VM638_04785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVM11772.1"
                     /translation="MAILPIKTFGDPVLRERARPVETFDDSLQRFAEDMIETMHDAPG
                     VGLAAPQVGRSIRMIVFDVGEGSHALVNPVLSGFEGEQVGEEGCLSIPGLYFPVKRAR
                     RVVCEARDTAGRPVRIEGEDLLARVLQHEVDHIDGVLFVDRLEGEERKQALAAIRDQA
                     LGLAESIPDPSRAL"
     gene            complement(4628..>4954)
                     /locus_tag="VM638_04790"
     CDS             complement(4628..>4954)
                     /locus_tag="VM638_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11773.1"
                     /translation="VVQTAEPGDAIVQALVRWDPWHLHAHEAARRAEAGFPPGHPIFR
                     LRGTPDLPAALGDIDPPPVSVLSTPVGDETVSLVTIRPDAVGAFRAWVMEHLGVVTRV
                     EANPDI"
BASE COUNT          692 a         1842 c         1776 g          644 t
ORIGIN      
        1 ggtcccgagc gcgcgggtgg acagcgcgac agcacgccgc atcgcttcgc gctcgtccac
       61 cgggtcccct cgcctgctcg agcgcgctcc ggggacgagc ggtcacggcg cacgcgggcg
      121 ccgccaccgg ccgcgcgccg cctctcctcc ggactgtcac cgtcggtccc ggagttccac
      181 cgggtccacc cccgactggc tatcgagggg tcgcggactg tcaccgccgg tggggaattc
      241 caccccgccc cggaaacgag ctacggaccc agtatacggc cggggctgct cagctcccgc
      301 ggcggtcgac ggcgaaggac gacccctgct cgcgcttcgc gaactccttg agctcggtgg
      361 cgaccgccac cgcctcgccg tgatgggaga accgccgccg gtcggtcgtc gcgacgccga
      421 tcgagatcga gacgaacggg taccgctcga cctgccctct gcggttctcg acctcgatgt
      481 gaccgcgctc acggtcctcg gggtcgtaga gcccggggat cttctcgtcg caggcggcga
      541 tgatcgcccg gcagagctcc tcggcacgat cgggtgagca gacgacgacg aagtcgtcgc
      601 cgccgacgtg gccgatgaac gcctcctggc cgacgacccg cagggcgtgc tcctggatgc
      661 atcgcgccgc gaaccggatc gcctcgtctc cccgcaggaa cccgtagtgg tcgttgtagg
      721 ccttgaagtt gtcgagatcg aggtagagga gagcgaacgg ctgcccctcc atgacgaggc
      781 ggtggagctc gtcctggatc cgcatgttgc cggggaggcc ggtcaagggc gagagcgcac
      841 gcatctcctg cgcccgccgc agggtgccct tcacgcgggc cacgagctcg acggggtcgc
      901 agggcttgat gacgtagtcg tccgcgccgg cggtgagccc gaggaccttg tcgaccgaca
      961 gggcccgagc ggtcagcatg atgatgctga cgttcgacgt ccgggggtcg gagcgcaggc
     1021 gctgggcgac ctcgaacccg tccatcttcg gcatcgtgac gtcgagcagc acgaggtccg
     1081 gcccgacggc gaaggcgcgc tcgagggctt cctctccgtc cgaggcgacg tgcgtctcgt
     1141 acccgtgggt ccgcaggttg agctcgacga accgggcgat atccggatcg tcgtcgacga
     1201 cgaggatctt ctcggccatc tagccccccg ccgcgaccct cgcgagcccc tccgccgccc
     1261 gcgccgggtc gtcagcacgg aagatcgagg aggcggcggc gagcatcgtc gccccggcct
     1321 cgatgcaccg cgctccggtg gcctcgttga tccctccgtc gacgatgacg tcgacgcgga
     1381 ggccccggcg gtcgatctcc gaccgaagcg cctccacctt ccgcaggctc gattcctgga
     1441 agggctggcc ggcgtacccc gtgctctcga gggtcatcac gatgacgttg tcgaggcctt
     1501 cgaggtgcgg gaacgcggcc cggatatcgg tctcggggtt gatcccgatg cccggcgcca
     1561 tcccggcggc gcgggcggcg gccgcgacct tcccggggtc ctcgacggcc tcgaggtgga
     1621 aggtgacgag atcggtcccc gcctcagcga agtcggcgaa caggcgctcg ggatgctcga
     1681 ccatcaggtg gcagtggagc ggaaggtcag agaccgggcg cagccactcg acgacgacgg
     1741 gaccgatcgt gagcggcggg acgaagtgcg cgtccatcac gtcgacgtgg atcaggtcgg
     1801 cgtagggctc cacccgcttg acctcgtcct cgaggcgggc gaagttcgcc gacaggatcg
     1861 atgctgccag gcgtgccacg cgcggcatcg tactacgcgc cgcggtgggc gaatcgagcc
     1921 acgaacatcc cgtcggtgcc gtgacggtgc ggccacagac gggccctcgc ggctggtcca
     1981 tccggcccgt cgaacggatc cggccgcaac tcggggaccc gtcggagcag cgcctcgcag
     2041 accgcgtccg tctccgcggc cgggaacgtg cagaccgagt agacgaggac gcccccgggc
     2101 cgcagcagcg ccgccgcccc cacgaggatc cgcgtctgga gccgcgccag cgccgggacg
     2161 tcctcccggc gcggccgcca caggagctcg ggccggcgcc gcgccgcgcc gatgcccgag
     2221 cacggcgcat cgaccagcac gcggtcgaag ctgcccggtc gcagagcggg ccgtgcggcg
     2281 tcctggacga ggagcctcgc ggagacgccg agccgctcga ccgtctccgc caccaggcgc
     2341 accctcccct ccccggcgtc cgcggcgagg accccgccgg cgcggcaggc gatgtccgac
     2401 gccttccccc ccggcccggc gcacagatcc gccacgcgct ccccgggctg cggatcgagg
     2461 acgtcgacca cccaggcgga ggactcgtcc tgggccgtca tccagccatc gtcgaagccg
     2521 ggcacgcgct ggggcgcgac cccgctggcc agcctgagcg tccgctcgtg cagccgaccg
     2581 cgttcgaagc tgatgcccgc gtctccgagg cgtcgctcga tctcggaggc gtcgctgcgg
     2641 caggggttca gccggatcgt cgtcggggcc ggggaggcga aggccatggc ggcctcttcc
     2701 gcctggtcgc cgagttgccg gcggagctcg cggaccgccc acggcgcgag tccgcttcgg
     2761 atcgcgagcc gatcgatcgc gccgcccgtc accgcccgcg gtccggaggc agcgagacgc
     2821 cgaaggacag cgttcacgaa gccgcgctcg gaggcgccga cgagcgcgac cgtctcgctc
     2881 accgcggcgt gatcgggcac gcgcgtgtgc aggagctggt aggcgccgag ccgcaaggcc
     2941 gcccgggccg tgggaggagc cttcgtcagc ggcttgcgca ggagcggagc gagggcgtga
     3001 tcgatcgccg gcagccgccg aagggtcccg tagacgagct ccgtgacgag gtcggcgtcc
     3061 tggtgcccga ggcgcgcgcg gcggatctgg ccccgcagcg cgaggttcga gtacgcgccg
     3121 tgctcggtga cccggcggat cacctcgagc gcgacgcgac gcgggtcagt ccgcgccgag
     3181 gacctctccc ggggccggtc ggtagccgcg gacgaagtcc ccgccgctga tccgccgccg
     3241 gccctccggg acgacctcga ggagcgcgac cgctccccgc gccgcggcga ccctgatccc
     3301 atcgttcgac acctccatga tcgcgccggg ctcgccggat ccatccaccg ccgccgcccg
     3361 aagcaccttc aggaccttcc ctcggtaccg tgtcctcgca ccgggatccg gcgccagcgc
     3421 gcggacccgt cgcacgagcg ccggcgcctc ctcgcgccag tcgagccact cctcctcccg
     3481 ggagatcttc ggcgcgagcg tcgcggtcgc cccgtcctgc gggcgctcgt tcgcctgacc
     3541 ccgccccagc aggtcgaggg cctcgaggag gaggtccgcg ccacggcgcg cgaggcggtc
     3601 gcccagcgtc cccgcgtcat cgtcctcgcg gatcggctcc tccgccgaga tcagcagcgg
     3661 tccggtgtcc atgccctcgt ccatccgcat gatcgtgacc cccgtggtcg tcgcccccga
     3721 caggatcgcg cgccgcaccg ggtcggcccc gcgcagcgaa ggcaggaggg agaagtgcac
     3781 gttcaaggga tacgcgaggt cgagcacgtg acgcgggagg atctcgccgt acgcgacgac
     3841 cacgagcgcc tcggctccgg cggcacgcag cgcctcgaac ccctcgcccg tccggacggt
     3901 ccctggctcc acgagcggca ggccgagcct ccgcgcggca tcggcgacgg ccgtcggacg
     3961 gagcttcccg ccccgccccg ccggccgggg cgggcgcgtc ccgacgatcg cgacgtggtg
     4021 gtcggagccg gcgagcgcct ccagggacgg gacggaccag gcgtcgctcc cgaggaaggc
     4081 gacccgcacc tacagcgcgc ggctcgggtc cgggatcgac tcggccagcc cgagcgcctg
     4141 atcgcggatc gccgccagcg cctgcttgcg ctcctcaccc tcgagccggt cgacgaagag
     4201 aacgccgtcg atgtgatcga cctcgtgctg caacacgcgc gcgaggagat cctcgccttc
     4261 gatccggacc ggacggccgg cggtgtcgcg agcctcgcac acgacccggc gcgcgcgctt
     4321 caccgggaag tacaggccgg ggatcgacag gcatccctcc tcccccacct gctcgccctc
     4381 gaaccccgac agcaccgggt tgacgagcgc gtgggacccc tcgcccacgt cgaagacgat
     4441 catccggatg ctccgaccca cctggggcgc ggcgaggcct accccagggg cgtcgtgcat
     4501 ggtctcgatc atgtcctcgg cgaagcgctg gagcgagtcg tcgaaggtct ccacggggcg
     4561 agcgcgctca cgcaggaccg ggtcgccgaa cgtcttgatc ggaaggatcg ccatcgcgct
     4621 ctcctgtcta gatgtcgggg ttcgcctcga cgcgggtcac cacgccgagg tgctccatca
     4681 cccaggcgcg gaaggcgccg accgcgtccg ggcggatcgt gaccaaggat accgtctcgt
     4741 cgccgacggg ggttgagagc acggagacgg gaggtgggtc gatgtccccg agcgccgccg
     4801 ggagatcggg ggtgccgcgg aggcggaaga tggggtgacc gggggggaac ccggcctccg
     4861 cgcgccgcgc cgcctcgtgc gcgtggaggt gccacgggtc ccagcggacg agcgcctgca
     4921 cgatcgcgtc gcccggctcg gcggtctgaa cgac
//
LOCUS       DATLGG010000168         2874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_168016, whole genome shotgun sequence.
ACCESSION   DATLGG010000168 DATLGG010000000
VERSION     DATLGG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168016"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VM638_04795"
     CDS             complement(<1..508)
                     /locus_tag="VM638_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11774.1"
                     /translation="MRRTRTAVALASLLLAALFAHPVGAQSTGEKLKEAKARLAKIDR
                     EAAAATAEYEAARGRLILTQEQMGELKKRMRDSRRRVTALQGRLGKRAREFYQLGPSS
                     TLELLLAADSFSEFSDRMVFLDQLAQEDADLVVRVSVLQEELRRRRSDLDTLAERQAE
                     TMETLEEKK"
     gene            622..1041
                     /locus_tag="VM638_04800"
     CDS             622..1041
                     /locus_tag="VM638_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVM11775.1"
                     /translation="MRDDGVIDTLGSLALFADLSRPELESVAHTWEEEWHPEGQRILR
                     QGFTGNNFYVILEGEAAVRIDGQDRATLGKGDFFGEVSVLLGEPPTADVVATRPLHCL
                     VLGGPDVEGFLIGLPKLMFRLLQAEARRLRNTIQWRA"
     gene            1029..2537
                     /locus_tag="VM638_04805"
     CDS             1029..2537
                     /locus_tag="VM638_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVM11776.1"
                     /translation="MARVARPSHPPGRYPVVIVGSGPGGIQMSYALSRLGVEHAFLSA
                     DDAPAGMFQRFPFFQRLISWTKPYAPHERGTRAYQRYDWNSLIAEDPAHNALVPRRMD
                     GTSSFPSREEMELGIADFVRRTRMKIRYGCRWEGTSREEDGSGFTIHTSDGDYRCGVF
                     VCAVGMTEAWKPDIPGLDRVPHYVNVKQARRYAGKDIVIIGKRNSGFELADGLLPWAR
                     TITLVSPRPTRLSIQIYSPAAARARYMQPYEEHVLGSGGVYVLDAAIERVSRGSDGWK
                     VIAQGTTAPGRIVLPADEVIAATGFGTPLRDLGDLGVKTFFSNRLPSQTPWWESATVP
                     GIYFAGSITQGSIGLKKYGQVSSSASVHGFRYNALVLARHLAETRFGVRPRRRKMRAD
                     AVAGYLLKEAAEAPELWNQRSYLCRSVVADAGRGIVDDGIVPLAQFVDAPGPDGVAVT
                     VETTDTGVMQPAIYVRSGGRVEEHILETDPLLRFRTRDNRARLDAILKGLFD"
     gene            2549..>2874
                     /locus_tag="VM638_04810"
     CDS             2549..>2874
                     /locus_tag="VM638_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVM11777.1"
                     /translation="MANPSDDELREIYASAKTIAVVGASADDSKPAHTIPRYLQRHGY
                     RVIPVNPRGGEILGEKVYEKLTDIEEPVDVVDVFRPSPETPDIARDAVQIGAKVLWLQ
                     QGIEHE"
BASE COUNT          428 a         1007 c         1015 g          424 t
ORIGIN      
        1 tcttcttctc ctcgagcgtc tccatggtct cagcctggcg ctccgcgagg gtgtcgaggt
       61 cgctccggcg gcggcgcagc tcctcctgca gcacgctgac ccggaccacg aggtccgcat
      121 cctcctgcgc cagctggtcg aggaagacca tccggtcgga gaactcgctg aaggagtcgg
      181 ccgccagcag cagctcgagg gtgctcgagg gaccgagctg gtagaactcc cgcgcccgct
      241 tcccgagccg gccctgcagc gccgtgaccc gtcggcggga atcccgcatc cgcttcttca
      301 gctcgcccat ctgctcctgc gtgaggatga gcctgccgcg tgccgcctcg tactccgccg
      361 tcgcggcggc ggcctcgcgg tcgatcttcg cgaggcgggc cttcgcctcc ttcagcttct
      421 cgcccgtgct ctgggcgccc acggggtgcg cgaagagcgc cgcgaggagg agggatgcga
      481 gggcgacggc ggtgcgggta cgtcgcatcg tcatatcgct cgaggtctcc ggtcgtatcg
      541 acacgggctg gggaaccggc ctcccgtacc ctatcaacgg ctccgcccga ccatcaacca
      601 gccggtggta ggctcccccc gatgcgggac gacggcgtca tcgacacgct cggcagcctg
      661 gcgctcttcg ccgacctctc gcgacccgag ctcgagtcgg tcgcgcacac gtgggaggag
      721 gagtggcacc cggaggggca gcggatcctc cgccagggct tcaccggcaa caacttctac
      781 gtgatcctcg agggggaggc ggcggtccgg atcgacggtc aggaccgcgc caccctcggc
      841 aagggcgact tcttcggcga ggtctcggtc ctcctcggag agccgccgac ggccgacgtg
      901 gtggcgacgc ggcccctcca ctgtctcgtg ctcggaggac ccgacgtcga ggggttcctc
      961 atcgggctcc ccaagctgat gttccgcctg ctccaggccg aggcccgccg cctgcggaac
     1021 acgatccagt ggcgcgcgta gcgcggccgt cgcacccgcc gggcagatac ccggtcgtca
     1081 tcgtcggcag cggtcccggg ggcatccaga tgtcgtacgc gctttcacgc ctcggcgtcg
     1141 aacatgcctt cctctccgcc gacgacgcac cggccgggat gttccagcgc ttcccgttct
     1201 tccagcgcct gatctcctgg acgaagccgt acgcgccgca cgagcgcggt acgagggcct
     1261 accagcgcta cgactggaac tccctgatcg ccgaggaccc cgcgcacaac gcgctcgtcc
     1321 cacggcggat ggacggcaca tcctcgttcc cgtcccgcga ggagatggag ctcggcatcg
     1381 cggacttcgt gcggcggacg cggatgaaga tccgctacgg gtgccgctgg gagggcacga
     1441 gccgcgagga ggatggctcc ggcttcacga tccacacctc cgacggcgac tatcgatgcg
     1501 gcgtgttcgt ctgcgccgtc gggatgacgg aggcgtggaa gcccgacatc ccgggcctcg
     1561 accgggtccc gcactacgtc aacgtgaagc aggctcggcg gtacgcgggc aaggacatcg
     1621 tcatcatcgg gaagcgcaac tccggcttcg agctggcgga cgggctgctt ccgtgggccc
     1681 ggacgatcac gctcgtctcg cctcgcccga cccgcctgtc gatccagatc tactcgccgg
     1741 ccgcagcgcg ggcgcgctac atgcagccgt acgaggagca cgtgctcggc tccggcgggg
     1801 tctacgtcct cgacgcggcg atcgagcggg tgagccgcgg gagcgacggg tggaaggtga
     1861 tcgcccaggg cacgaccgcg cccggaagga tcgtgctgcc ggccgacgag gtgatcgccg
     1921 cgaccggctt cgggacgccg ctgcgcgacc tcggcgacct cggcgtgaag accttcttca
     1981 gcaaccgcct cccgtcgcag acgccgtggt gggagtcggc gacggtgccg ggcatctact
     2041 tcgcggggtc gatcacccag ggatcgatcg gactcaagaa gtacgggcag gtcagcagct
     2101 ccgcgtcggt ccacggcttc cgctacaacg cgctcgtgct cgcgcgccac ctcgccgaga
     2161 cgcggttcgg cgtccggccg cgccgccgga agatgcgggc ggacgcggtg gccggatacc
     2221 tgctcaagga ggccgcggag gcgccggagc tgtggaacca gcgttcgtac ctgtgccgct
     2281 cggtggtggc ggatgcgggg cgcgggatcg tcgacgacgg gatcgtgccc ctcgcccagt
     2341 tcgtcgacgc tccgggaccc gacggcgtcg ccgtcacggt ggagacgacc gacaccggcg
     2401 tgatgcagcc ggcgatctac gtgcgctccg ggggccgggt cgaggagcac atcctcgaga
     2461 ccgatccgct gctgcgcttc cgcacgcggg acaaccgcgc gcggctcgat gcgatcctga
     2521 aggggctgtt cgactgagag gagcggagat ggcgaacccg tccgacgacg agctgaggga
     2581 gatctacgcg agcgcgaaga cgatcgcggt ggtgggcgcg tctgccgacg actcgaagcc
     2641 ggcgcacacg atcccccggt acctccagcg gcacgggtac cgcgtgatcc cggtgaaccc
     2701 gcgcggcggc gagatcctcg gcgagaaggt ctacgagaag ctgacggaca tcgaggagcc
     2761 ggtcgacgtc gtcgacgtct tccgcccctc gccggagacg cccgacatcg cgcgggacgc
     2821 ggtgcagatc ggggcgaagg tgctctggct ccagcagggg atcgagcacg agga
//
LOCUS       DATLGG010000169         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_227362, whole genome shotgun sequence.
ACCESSION   DATLGG010000169 DATLGG010000000
VERSION     DATLGG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227362"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..2065
                     /locus_tag="VM638_04815"
     CDS             128..2065
                     /locus_tag="VM638_04815"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVM11778.1"
                     /translation="MRHAGRRIWSSALIALVLASLAALGLGAGVFHGLQLRLTDTLFP
                     AVAADPRIAVVGFDDRSLSEVQRWPWDRDLHARLIDALAADGAALVGYDVTFSEPSEP
                     EDDRELGRAIREAGNVVLGATATFEGRGERVLRAARFEPPVPAIAEGAAAIAHVNVIP
                     DPGDGVVRSIPLVVETPDGELVPSLSFALYLLEGRAGGPITFRPEGIQAGGTLVPTGR
                     QANLDVNFAEGFDVYPAVDVLEGNIPPGTFRDRIVLVGATAIGLGDIKITPLDKASGQ
                     PGVLVHANALSTVRNAAYLTPLGRAPSVALVFLLALLVALAVAFLGVWLSPIVTAGIG
                     VGYAMLAFQRFDRGQVLNLVYPAIAIVLAYLAALAFRYLTEVRERRRVTRTFTRYLAG
                     DVVEEVLASPEEAFATLQGVERPISVLFADLRGFTSASEGATPREVVAALNVYLDAMT
                     QAVMAEGGTIDKFMGDCVMAFWNAPREDPEHADKAVRAAVGMLDRIDRAVVEKEEARL
                     LQVKGCGVGVATGEAVVGNIGSSERLDYTVVGDTVNTASRLCGVAAAGQVVVTAETAR
                     QVEGKVRLADLPPLAVKGKREPLRVFQVLREGQEAQAFEEGAVLDATEDKGHFETPVE
                     APPKAAGYTPVEPRPEQPPQA"
BASE COUNT          292 a          795 c          844 g          333 t
ORIGIN      
        1 cccccacctc ctcctccgga ggacccgccg aagccgatcc acgagcaggt gatcgagcgg
       61 gtgtgcagcg aggtcgagaa ggtgagcggg caggagctcg gcccgccctg ctaggctccc
      121 ggcctgcatg aggcatgcag gccgccgcat ctggtcctcc gcgctgatcg cgctcgtgct
      181 cgcttcgctc gccgcgctgg gcctcggtgc cggggtcttc cacgggctcc agctacgcct
      241 gaccgacacc ctgttcccgg cggtggcggc cgacccgcgg atcgccgtcg tcgggttcga
      301 cgaccgcagc ctcagcgagg ttcagcggtg gccgtgggac cgtgacctgc acgcgcggct
      361 gatcgacgcg ctcgcggcgg acggcgcggc gctcgtcggc tacgacgtca ccttctcgga
      421 gccgagcgag ccggaggacg accgggagct cggacgggcg atccgcgagg cggggaacgt
      481 cgtgttgggc gcgaccgcca ccttcgaggg acggggcgag cgggtgttgc gcgccgctcg
      541 gttcgaaccg cccgtcccgg cgatcgccga gggcgcggcg gccatcgccc atgtcaacgt
      601 gatccccgac ccgggtgacg gcgtcgtgcg ctcgatcccc ctcgtcgtcg agacccccga
      661 cggcgagctg gtcccgtcgc tgtcgttcgc gctctacctc ctcgagggtc gcgcgggcgg
      721 cccgatcacg ttccggcccg aggggatcca ggcgggcggc acgctcgttc ccacggggcg
      781 tcaggcgaac ctcgacgtga acttcgccga aggcttcgac gtctacccgg ccgtcgacgt
      841 cctcgagggg aacatcccgc cggggacctt ccgcgaccgc atcgtgctgg tgggcgcgac
      901 ggcgatcggc ctgggggaca tcaagatcac gccgctcgac aaggcatccg ggcagccggg
      961 ggtgctcgtg cacgcgaacg ccctcagcac cgtgcgcaac gccgcgtacc tgacgcccct
     1021 cgggagggcg ccctcggtcg cgctcgtctt cctgctcgcg ctgttggtcg cgctggcggt
     1081 cgccttcctc ggcgtctggc tttcgccgat cgtgacggcg gggatcggcg tcggctacgc
     1141 gatgcttgcg ttccagcggt tcgatcgggg gcaggtcctt aacctcgtct acccggcgat
     1201 cgcgatcgtg ctcgcgtacc tcgcggccct cgccttccgc tacctcacag aggtccggga
     1261 gcggcggcgc gtgacgcgga ccttcacgcg ctacctcgcc ggcgacgtgg tcgaggaggt
     1321 gctcgcctcg cccgaggagg cgttcgccac ccttcagggc gtcgagcggc cgatctcggt
     1381 cctgttcgcg gacctccggg ggttcacgtc cgcgtcggaa ggggcgaccc cccgggaggt
     1441 ggtggccgcg ctgaacgtct acctcgacgc gatgacgcag gcggtgatgg cggagggcgg
     1501 aacgatcgac aagttcatgg gcgactgcgt gatggcgttc tggaacgcgc cccgggagga
     1561 tcccgagcac gcggacaagg ccgtccgcgc ggccgtcggc atgctggacc ggatcgaccg
     1621 ggccgtcgtc gagaaggagg aggcgcgcct cctccaggtg aaggggtgcg gggtcggggt
     1681 ggcgaccggc gaagcggtcg tcgggaacat cggctcgagc gagcgcctcg actacaccgt
     1741 cgtcggggac acggtcaaca ccgcgagccg tctctgcggc gttgccgctg ccggccaggt
     1801 ggtcgtgacg gcggagaccg cccggcaggt ggaggggaag gtgcgtctcg cggacctccc
     1861 gccgctcgcc gtcaagggga agcgggagcc cctgcgcgtc ttccaggtcc tgcgcgaggg
     1921 gcaggaggct caggccttcg aggaaggcgc ggtgctcgac gcgacggagg acaaggggca
     1981 tttcgagacg ccggtggagg ccccgcccaa ggccgccggc tacacacccg tcgagccccg
     2041 ccccgagcag cctccccaag cctgaccctg tcccgccggg gggcccggcg accccccgcc
     2101 cactgttggg aaggtcggcg gcaccctgtt cgcgagctgc atgctgctgt gacgtcatgg
     2161 cgtcactccg gggacgaacg cgctggtcgt gggcgcgaca tgctgccatg gcgccatcgc
     2221 agcatgatgg tcggcttggg agggaggccg ccgctctcgg aggg
//
LOCUS       DATLGG010000170         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_57980, whole genome shotgun sequence.
ACCESSION   DATLGG010000170 DATLGG010000000
VERSION     DATLGG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57980"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VM638_04820"
     CDS             <1..789
                     /locus_tag="VM638_04820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVM11779.1"
                     /translation="GWGRIPGGKGLVGRIGRDAILLTTVSEWTRQILRDAVGPRPRIE
                     LLPPGIDTDLFHPSVSDEEVRDRHRLWDDPVICCVSRTVARKGQDQVIEALPRIAAEV
                     PNVRFLVVGSGPHERKLWELARARRVEHRVVFAGEVPYHELPMYFRAGEVFAMPSRPR
                     WFGLEVEALGAVYLQAYAVGRPCVIGASGGAPEAALDGDAGIVVDGTSAGEVAGAILS
                     LLRDPERAEKMGAAGAAWVHRDLGWGTIAARLRDLLADCLRGRR"
     gene            786..1787
                     /locus_tag="VM638_04825"
     CDS             786..1787
                     /locus_tag="VM638_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HVM11780.1"
                     /translation="MTIRAKAPLRISFAGGGTDVEPYPTEHGGAVLSATIDLYAFASL
                     QPRDDGEHSVETDDGGSARYASRQDLVFDGHLDLVKAVLRHTAPDRGFDLHLSSDAPP
                     GSGLGTSSALVVAMLAALGEATREPVTPYELASRAYQVERVDLRQAGGMQDQYASAFG
                     GFNFIEFFGPDRVIVNPLRIRSDVLNELHGSLLLCHTGITRTSGGILRRQVAGYRSGE
                     TVDTLGRIRDLAVEMKEALLVGDLDRFAKGLTRGWEEKRTLAEGITNDRVEDLYQRAM
                     ASGAIAGKLLGAGGGGFLLLFCPFRRRARVAEAMESAGARVVRFHFEERGAETWRAR"
     gene            1772..2353
                     /locus_tag="VM638_04830"
     CDS             1772..2353
                     /locus_tag="VM638_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVM11781.1"
                     /translation="MASALSPARSEEAAPARPAVFLDRDGTLIEDPGYLGDPDGVVLI
                     EGVPDALRRLASTGYALVVISNQAGVARGLFDEDAVRAVNARVEELLAEHGARIDAWH
                     WCVHHPDVTGPCACRKPGTELLERAARELGLDLGASWMVGDHPTDVQAGIRAGGRGIM
                     VLTGHGGHGTAPPGHPVVADLRAAADLILGAGR"
     gene            2408..2722
                     /locus_tag="VM638_04835"
     CDS             2408..2722
                     /locus_tag="VM638_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVM11782.1"
                     /translation="MTLEVEHTGDTFRLKGELDIAAEEALMQALEPELEAGREVILDL
                     GGLEFIDSSGVRCLIRAANAVGSGGRVVLRDPLPPVRRVFELMRVDTIPNVAVEPSGG
                     SP"
BASE COUNT          356 a          943 c         1043 g          381 t
ORIGIN      
        1 gggtgggggc ggatcccggg cggcaaggga ctcgtggggc ggatcggccg cgacgccatc
       61 ctgctcacga ccgtgagcga atggacccgc cagatcctgc gtgacgcggt ggggccccgg
      121 ccgcgcatcg agctcctccc accgggcatc gacacggatc tcttccaccc gagcgtctcc
      181 gacgaggagg tccgggacag gcatcgcctc tgggacgacc ccgtgatctg ctgcgtctcg
      241 cgcacggtcg cgcggaaggg ccaggaccag gtcatcgagg cgctcccccg gatcgccgcc
      301 gaggtcccga acgtgcgctt cctcgtcgtc gggtcggggc cgcacgaacg gaagctctgg
      361 gagctcgcgc gtgcccggcg ggtggagcat cgcgtcgtgt tcgcgggcga ggtcccctac
      421 cacgagctgc cgatgtactt ccgggccggc gaggtcttcg cgatgccgag ccggccgcgg
      481 tggttcggcc tcgaggtcga ggcactgggg gccgtgtacc tccaggccta cgcggtcggt
      541 cgcccgtgcg tgatcggcgc ctccggcggc gccccggagg ccgcgctcga cggcgacgcg
      601 gggatcgtcg tcgacggcac gagcgccgga gaggtcgcgg gcgcgatcct ctcgctcctg
      661 cgggacccgg agcgcgcgga gaagatgggc gccgccggcg cagcgtgggt ccaccgcgac
      721 ctcggctggg gaacgatcgc cgcccggctc cgggacctgc tggccgattg ccttcggggg
      781 cggcggtgac gatccgagcc aaggcgcctc tgcgcatcag cttcgccggc ggcgggaccg
      841 acgtcgagcc gtaccccacc gagcacggcg gcgccgtcct ctcggcgacg atcgacctgt
      901 acgcgttcgc ctcgctgcag ccgcgggacg acggcgagca ttcggtcgag accgacgacg
      961 gcggctccgc ccgctacgcc tcccggcagg acctcgtctt cgacggccac ctcgacctcg
     1021 tgaaggcggt cctgcggcac acggcccccg accgcgggtt cgacctgcat ctgtcgagcg
     1081 acgccccgcc cggctcgggt ctcgggacct cgtccgcgct cgtggtcgcg atgttggcgg
     1141 ccctcggaga agcgacgcgc gagccggtga cgccgtacga gctcgccagt cgtgcctacc
     1201 aggtggagcg ggtcgacctg cgtcaggccg gcgggatgca ggatcagtac gcgtccgcgt
     1261 tcggcgggtt caacttcatc gagttcttcg ggcccgaccg ggtgatcgtg aacccgttgc
     1321 ggatccggtc ggacgtcctc aacgagctgc acgggtcgct cctgctgtgc cacacgggca
     1381 tcacccggac ctcggggggg atcctgcgcc ggcaggtcgc cgggtaccgc tccggcgaga
     1441 cggtcgacac gctcgggcgg atccgggacc tcgcggtcga gatgaaggag gcgctgctgg
     1501 tcggcgacct cgaccggttc gcgaagggcc tgacgcgcgg gtgggaggag aaacgcacgc
     1561 tcgcggaggg gatcacgaac gatcgcgtcg aggacctcta ccagcgggcg atggcgtcgg
     1621 gagcgatcgc ggggaagctc ctgggggccg ggggcggagg gttcctcctc ctgttctgcc
     1681 cgttccggcg ccgcgcccgg gtcgcggagg cgatggagtc cgcgggcgcc cgggtcgttc
     1741 ggttccactt cgaggagcgg ggggcggaga cgtggcgagc gcgctgagcc cggcgcgatc
     1801 ggaggaggcg gcccccgcgc gcccggcggt cttcctggac cgcgacggaa ccctgatcga
     1861 ggatccgggc tacctcggcg accccgacgg cgtggtgctg atcgagggcg tgcccgatgc
     1921 gctccgacgg ctcgcctcga ccgggtacgc cctcgtcgtc atctcgaacc aggccggcgt
     1981 cgcccgcggg ctcttcgacg aggacgcggt gcgggcggtg aacgcgcggg tggaggagct
     2041 gctcgcggag cacggggcca ggatcgacgc gtggcactgg tgcgtgcacc atccggacgt
     2101 gaccggcccc tgcgcctgcc gcaagccggg gaccgagctg ctcgaacggg cagcgcgcga
     2161 gctcggcctc gacctcggcg cgtcgtggat ggtcggggat cacccgaccg acgtccaggc
     2221 agggatccgg gccggcggac gcggcatcat ggtcctgacg ggccacggtg gtcacggaac
     2281 cgcgccccca ggccatccgg tggtcgccga tcttcgggcc gcggcggacc tgatcctggg
     2341 cgcaggccgc tagacgcgtc aacccgactg ggcgtagatt cagcgctatg ggagcggagg
     2401 gaccttggtg acgctcgagg tcgagcacac cggcgacacg ttccgcctca agggcgagct
     2461 ggatatcgcc gctgaggagg cgctgatgca agccctcgag ccggagctgg aggcgggccg
     2521 cgaggtcatc ttggacctcg gtggactcga gttcatcgac agcagcgggg tccgctgcct
     2581 gatccgggcg gcgaacgccg tcggctccgg cggccgggtc gtcctgcgcg acccgctccc
     2641 accggtccgc cgggtgttcg agctcatgcg ggtggatacg atccccaacg tggcggtcga
     2701 accgagcggc ggctccccat gat
//
LOCUS       DATLGG010000171         2383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_137527, whole genome shotgun sequence.
ACCESSION   DATLGG010000171 DATLGG010000000
VERSION     DATLGG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137527"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..380)
                     /locus_tag="VM638_04840"
     CDS             complement(81..380)
                     /locus_tag="VM638_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11783.1"
                     /translation="MKRLLTGIGGALGLAALVRLVRGRRSAPADTGAVPEPAEELRRK
                     LAEAREAAEDRDEFDASEGQPVDEVDEPRSIEERRRAIHERAQEALDEMRRLDDA"
     gene            502..870
                     /gene="rpsF"
                     /locus_tag="VM638_04845"
     CDS             502..870
                     /gene="rpsF"
                     /locus_tag="VM638_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVM11784.1"
                     /translation="MIDYEILLMLDPDLGEERQEEIVKRTRGLVEGGGGTWAGQDSWG
                     RRKLAYEIDHKAEGVYHLLSFTAEPETLDEISRVLKIDDGVMRHMATRRYKPGSTQPV
                     AAGGPAREDAADTDIPLEEE"
     gene            870..1307
                     /locus_tag="VM638_04850"
     CDS             870..1307
                     /locus_tag="VM638_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVM11785.1"
                     /translation="MPANINSVVLVGNLTRDPELRHTPSGMAVCSLRLAVNSRRKDET
                     GQWADKPNYFDVTVWGQQGENCAQYLAKGRPVGVQGRLEWREWDAQDGTKRQAVEVVA
                     DNVQFLGGRGDGESGGGGFVPQGAAAPAAADFPSSAADDDIPF"
     gene            1316..1585
                     /gene="rpsR"
                     /locus_tag="VM638_04855"
     CDS             1316..1585
                     /gene="rpsR"
                     /locus_tag="VM638_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVM11786.1"
                     /translation="MAQPKTQERSRKRPGSAAAPGRRKSCLFCKDKVEEVDYKNVNQL
                     RRYISEKGKIRNRRITGACRRHQRQVAVAVKRAREMALLPYVEQR"
     gene            1585..2250
                     /gene="rplI"
                     /locus_tag="VM638_04860"
     CDS             1585..2250
                     /gene="rplI"
                     /locus_tag="VM638_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HVM11787.1"
                     /translation="MDVILLKDVEKVGLRGEVVSVARGYARNYLLPRKLAEAATPGRV
                     AELKRIDEQRARHEARSVEQAREVADALGKTVLRFEVKAGPTGSLFGSVTATDIADEL
                     WRTRKIRIDRRKIEIDTLKRIGRYSVPVHVFEDVTAEVKTLVVPEGGELPPEEELAAM
                     EAAERAAAGEEPAASEVPADEAPLAEAEGPAEIEALEEAAPAEAPADGSDEGAEAEET
                     AAP"
BASE COUNT          373 a          846 c          862 g          302 t
ORIGIN      
        1 cagcacgctc gtaccgacgt tcccggtcgc tccgacgacg acgacgtgca tgcgggcggc
       61 gtgcccgcgt ggggcggccg ctatgcgtcg tcgagccggc gcatctcgtc gagcgcctcc
      121 tgcgcgcgct cgtggatcgc ccgccgccgc tcctcgatcg accgcggctc gtcgacctcg
      181 tcgacgggtt gcccctccga cgcgtcgaac tcgtcgcggt cctccgctgc ctcgcgtgcc
      241 tccgcgagct tccgccgcag ctcctccgcc ggctcaggca cggctccggt gtccgcgggc
      301 gcgctccgcc tcccgcggac gagacgcacg agcgccgcga gcccgagcgc cccgccgatc
      361 cccgtgagca gccgcttcat cgacccgatg ctactctgac ggcgcttccc gcctcgtctc
      421 gcacgcgctc gttcccgcac gtgcctgccg gcgaggccgc ctaagaccag gggaagccat
      481 gccgaaagga gccaggggaa cgtgatcgac tacgaaatct tgctgatgct ggaccccgac
      541 ctgggcgagg agcgccagga ggagatcgtc aagcgcacgc gcggcctcgt cgagggcggc
      601 ggcggcacgt gggccggcca ggactcgtgg gggcggcgca agctcgccta cgagatcgac
      661 cacaaggcgg agggggtcta ccacctgctc tcgttcacgg ccgagccgga gacgctcgac
      721 gagatctcgc gcgtgctgaa gatcgacgac ggcgtgatgc gccacatggc gacacgtcgg
      781 tacaagcctg gctcgactca gccggtagcg gcgggcggcc ctgcgcgcga ggatgcagcg
      841 gacaccgaca tcccgctgga ggaggagtaa tgcccgcgaa catcaacagc gtcgtcctcg
      901 tcggcaacct gacgcgcgac ccggagctgc gccacacccc gtcggggatg gccgtctgct
      961 cgctgcggct cgcggtcaac tcacggcgca aggacgagac cggccagtgg gccgacaagc
     1021 cgaactactt cgacgtcacc gtgtggggcc agcagggcga gaactgcgcg cagtacctcg
     1081 cgaagggccg ccccgtcggc gtccagggcc gcctcgagtg gcgcgagtgg gacgcgcagg
     1141 acggcacgaa gcggcaggcg gtcgaggtcg tcgccgacaa cgtccagttc ctgggcggcc
     1201 gcggtgacgg cgagtccggc ggcggcggct tcgttcccca gggcgcggct gcgcccgctg
     1261 cggcggactt cccgtcctcc gcagccgacg acgacattcc gttctaggag agacgatggc
     1321 acagccgaag acacaggagc ggtcgcgcaa gcgccccggc tcggccgccg cccccgggcg
     1381 ccgcaagagc tgcctcttct gcaaggacaa ggtcgaagag gtcgattaca agaacgtcaa
     1441 ccagctccgg cgctacatct cggagaaggg caagatccgc aaccgccgca tcacgggcgc
     1501 atgccggcgc caccagcgcc aggtcgcggt ggccgtgaag cgggcgcgcg agatggcgct
     1561 cctgccgtac gtggagcagc gctagtggac gtcatcctgc tcaaggacgt cgagaaggtc
     1621 ggcctccgcg gcgaggtcgt cagcgtcgcc cgcggctacg cgcgcaacta cctgctcccg
     1681 cggaagctgg ccgaggcggc gacgcccggc cgggtcgcgg agctgaagcg gatcgacgag
     1741 cagcgcgcac ggcacgaggc acggtcggtc gagcaggcgc gggaggtcgc tgacgccctc
     1801 ggcaagaccg tgcttcgctt cgaggtgaag gcgggcccga ccggttcgct gttcggctcc
     1861 gtgaccgcga ccgacatcgc cgacgagctc tggcggacgc ggaagatccg gatcgaccgg
     1921 cgcaagatcg agatcgacac gctgaagcgg atcggccgct acagcgtccc ggtccacgtc
     1981 ttcgaggacg tgacggcgga ggtgaagacg ctcgtcgtcc ccgagggcgg agagctgccg
     2041 ccggaggagg agctcgccgc gatggaggcc gccgagcgcg cggctgccgg cgaggagccg
     2101 gcagcctccg aggtgcccgc cgacgaggcg ccgctcgcgg aggccgaggg ccccgccgag
     2161 atcgaggcgc tcgaagaggc cgctcccgcc gaggcgcccg cggacgggtc ggacgagggc
     2221 gcagaggccg aggagacggc ggcgccgtag gtcctttcca cagcgtgctc cacaacgtgt
     2281 ggagcacgct gtgaacttcc cgcatcttcc gccctgtccg ctgtggacag gtgccacggt
     2341 cggtgcccgt tcccgtccga gcccttgggg atactgacgg aac
//
LOCUS       DATLGG010000172        11000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_27316, whole genome shotgun sequence.
ACCESSION   DATLGG010000172 DATLGG010000000
VERSION     DATLGG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27316"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VM638_04865"
     CDS             complement(<1..199)
                     /locus_tag="VM638_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11788.1"
                     /translation="MTKPDVFLGPTYLKDDDFKKAIGAVDHQVDARYAWDTGIAISEY
                     LRGLKEGKILARYCRVCRRTLV"
     gene            complement(226..1389)
                     /locus_tag="VM638_04870"
     CDS             complement(226..1389)
                     /locus_tag="VM638_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HVM11789.1"
                     /translation="MGQRVAVVGAGMTKFVRRAQETGKELAWQAAKAALESAEMTLDD
                     IDCVAMGSAPDAFDGVHMKGEYLADGAGAWGKPYFRTFVGGGTGVFVTIHGWYQVASG
                     MFDTCLVVAEEKMSSVQPHAQGAFITIFDNILERPLGPNLLWIFALEMNRYMTVHGLE
                     KKDIAAVSVKNKRNAADHPAALLGDPNITVDDVLKSETLAWPVQRLDVSPVSDGAVAI
                     VLAAEHKAKRLTNRPVWIDGVGWHLDTAYWTNRDLYYPRYVEYAARQAYDMAGIREPR
                     KEIHIAEAYDPFDYKELHHLEGLMLFDKGKAPEAMKDGVLNRDGDLPSTPSGGLLGVG
                     NPIAAAGLMKIAELYWQLSGQAGKRQVPGDPRKGVAQAWGDLMQVGTVVVMGK"
     gene            complement(1390..1761)
                     /locus_tag="VM638_04875"
     CDS             complement(1390..1761)
                     /locus_tag="VM638_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVM11790.1"
                     /translation="MKLGPLDHVYYWTEDMDRAVSFYEGTLGLRLLRREGGSWAMFDT
                     GSDVHLALHGAIEGRRTETGGATAVFRVEDLDAARADLAGRGVPLDDHLGEVEGYARF
                     ASLVDPDGNRVQMIEYARRGS"
     gene            complement(1758..2924)
                     /locus_tag="VM638_04880"
     CDS             complement(1758..2924)
                     /locus_tag="VM638_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HVM11791.1"
                     /translation="MGNRVAIVGIGHAGFAPITAGLSYKELTFEAAVRAYDDAGIDPR
                     KDVDQFVTAAEDFWEGTSIFDEYVPDQIGAALRPVQTVTADGLYAIATAVMLIRSGAA
                     KVVAVEGHSKASDILSLGSIHQFAMDPVYNRPLDVSPLAVAGLEMNRYLEATGQTDEQ
                     CALVVEKNRRNALDNPHAAHPADLSAEDVLGSDPTWWPLRRLDAAQRADGCIVAVLAD
                     GDVARDLSDSPVWVVGIGWDTGSPSLESRSWEEADYVKRAAKRAYRMAGLSNPANEVD
                     LAEVDDLYSYRELMSLEALGLARQGEAGPMLEEGAFAADGDLPVNVSGGSLGVGNLFE
                     ANGLARTFECVLQLRGEAGERQLEDADVAIAQSWRGVPTSSAAVAVYAADEVVA"
     gene            complement(2970..3737)
                     /locus_tag="VM638_04885"
     CDS             complement(2970..3737)
                     /locus_tag="VM638_04885"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HVM11792.1"
                     /translation="MSQHSLEQAVQRAGIDAPPVWFDETASTNDEALVLAARGAPSWT
                     VIATGHQTAGRGRERRTWHSVPGKLLQFTLLLRPPLHPAEASLTSLLAAAEMARACRE
                     VAGVAVRSKWPNDLLAGRRKVGGILTEARVQGEELEVLFLGVGVNVAQAEEDFPAELA
                     EVATSLAIEGSPAEPLELLAGFLHGFRAAWAPGSAGHGRRIVSAYREICDTIGRRVRA
                     TTVDGRVVEGLAERIDDRGGLVVDGRTVSFGEIAHVG"
     gene            3769..5493
                     /locus_tag="VM638_04890"
     CDS             3769..5493
                     /locus_tag="VM638_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVM11793.1"
                     /translation="MAETRVLIANRGEIAVRVIRTCRELGIPTIAVYSDADRDGLHVE
                     MADEAYRIGPATPSQSYLNVAAILDAAARGRATHVHPGYGFLSENPDFARACADAGLT
                     FIGPPPEAMEMMGDKAAARRAAESVGVPVIPGVSEPVSLEQAREVASSIGYPMAVKAA
                     FGGGGRGMHLIEDASALDESLERSAREAKAYFGRPEVYLERYIARAHHVEAQIIADTQ
                     GNISFLGERDCTLQRRYQKLLEESPSPVVDESLRTRIGEASLAIAKAAGYANAGTVEF
                     LVDDDGAFYFLEVNARLQVEHPVTEMVTGLDLVKLQLAVAHGEKVDLTPDLRGHAIEC
                     RINAEDPTKDFLPGPGLLTRFRPPGGPFVRLDTGFGEGKVVPGDYDSLFAKLLVWGED
                     REAARRRMLRAFDEFEIEGVPTTIEFYRWAMETLTFREASHTTKWVEGALAEGQFQPA
                     GPEAAATAAPPVRPARVIVELDGRRVPVQVWGDDLPAPPEPPAAVSGGGGAHGHDVLV
                     APMQGTILKVLVEEGQEIAAGDSVCILEAMKMENHIASTRAGVIEKVAVKPGDVVDGG
                     QLLVGFAD"
     gene            complement(5494..6120)
                     /locus_tag="VM638_04895"
     CDS             complement(5494..6120)
                     /locus_tag="VM638_04895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM11794.1"
                     /translation="MLPDDLDGLRVAELGCGTAYVSAWLARRGARPVGLDLAERQLET
                     AARLQREHGLRFPLVHGDAEHPPFADAAFDLVINEYGAALWCDPYRWIPEAARILRPG
                     GSLIFLTMGVLMALTIPDLGTDGPASDRLLRPYFGMHRFEWEDDESVEFHLGHGDWVR
                     LLRETGFEIEDLIEVRPPEGSTTRYPWAELDWARKWPVEEVWKARKRR"
     gene            complement(6264..7112)
                     /locus_tag="VM638_04900"
     CDS             complement(6264..7112)
                     /locus_tag="VM638_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM11795.1"
                     /translation="MKIGIGLPNTIPGTPGRMLVEWARHAEDLGFSSLATIGRVAYPS
                     YDTMATLAAAGAVTERIGLVSNVLLIPTHDPVMIAKAAASVDQISGGRFTMGIGVGTR
                     DDDYQAAGVPFEERGKRADRSLEVIHRAWAGELVEGARKPVTPAPIAGTVPIIVGGSS
                     DAAIRRTVRWGIGWTAGGSAPQQVGPFAERVRAAWKEAGREGEPKIMALAYYALGGAE
                     QEGLASLTDYYGDFGPKIFEYMPKSPEDIRTTAKMFEDAGVDELVFDPTVADLKQVDL
                     LAEAAL"
     gene            complement(7109..7792)
                     /gene="npdG"
                     /locus_tag="VM638_04905"
     CDS             complement(7109..7792)
                     /gene="npdG"
                     /locus_tag="VM638_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HVM11796.1"
                     /translation="MTVAIVGGTGDLGFGLALRLAAAGQEVVIGSRSAEKAGDAVARA
                     QEVLGPDAPVDGRANPEAVAAGDVTFVTVPFEGQAATYRALKEAWRDGAIVCDTTTPL
                     ATAVGGAATQVLRPWQGSAAEQAKALLPPGVRLASGFHSVGAEALEALDGDLQGDVLL
                     CGDGEAKAAVGGLVEKIGMRWVDCGPLTMARILEPLTAVMIRVNKTYGLRDTGIRLTG
                     RERWGRSDA"
     gene            complement(7789..9354)
                     /locus_tag="VM638_04910"
     CDS             complement(7789..9354)
                     /locus_tag="VM638_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVM11797.1"
                     /translation="MAEDERRTDSGIEIRPLYEAPDRPPELGRPGEPPFTRGIYPSMY
                     RGRPWTMRQYAGMGTAEETNRRFRYLLDHGQTGLSVAFDLPTQMGYDSDHPRAEGEVG
                     RTGVAIDSIEDMKTLFAEIPLDQVTTSMTINATAAILLLLYELTAEEQGIGPEKLGGT
                     VQNDILKEYAARGTYIYPPKPSMRIITDLFAYCRERIPRWNTISISGYHMREAGSTAV
                     QEVAFTLANAIAYVQAALDAGLKVDDFGPRLSFFFACHMNFFEEVAKFRAARRMWAKI
                     MSERFGAKDAKSLMLRFHTQTGGATLTAQQVENNVVRTALEALAAVFGGTQSLHTNSF
                     DEALALPSETAARVALRTQQVIAYETGVIDTVDPLGGSYFVEALTDEIEAGAWDYIEK
                     IDAMGGSVPAIERGWIQDEIHEAAFRIQQGVESGERVVVGVNRFVSEDEEPVELQRLD
                     EEQVRRQSERLREIRAGRDNAEVERALKEVEETARGTDNLLPPMREALKARATLGEAS
                     DALRRVFGEHRPA"
     gene            9420..9815
                     /gene="sdhC"
                     /locus_tag="VM638_04915"
     CDS             9420..9815
                     /gene="sdhC"
                     /locus_tag="VM638_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HVM11798.1"
                     /translation="MAKRTSELGGIYKAGTGQWAWLAHRITGVGILFFLFAHIVDTAL
                     VAWGPETYNKVIALYHHPIVQIMEIGLVGAVIFHGVNGIRIMIMDFWPKSLLYHRQLT
                     WATLLVFFAAMGPVTWIMGDRVLHALGWL"
     gene            9815..10219
                     /locus_tag="VM638_04920"
     CDS             9815..10219
                     /locus_tag="VM638_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HVM11799.1"
                     /translation="MAVATREPTLPAEPRTRRRGFELWSWLFMRISGILLLFLAVGHV
                     LIMHVMEEGVERVNFDFVAGRWANPLWKTWDWAMLSLALLHGLNGMRVIIQDYVRRPG
                     WRLFWNSTFWVLGFGLFLLGTIIVLTFDPTVF"
     gene            10221..>11000
                     /locus_tag="VM638_04925"
     CDS             10221..>11000
                     /locus_tag="VM638_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVM11800.1"
                     /translation="MTIEHTYDAVIVGAGGAGLRAAIEAAGSVKTAVISKLYPTRSHT
                     GAAQGGMCAALGNVEEDYPEWHAFDTVKGGDYLVDQPAAEIMANEAVDAVIQLEHWGF
                     PFNRTPDGRIDQRRFGGHTRNHGEAAVRRACYAADRTGHMILQTLYQQCIKRDVRFFN
                     EFYVLDLLMQDGRAAGVVAYELSSGELHVFHAPAVLFATGGYGKMFKVSSNAHTLTGD
                     GPAVCFRRGIPLEDMEFFQFHPTGIYKMGILISEAVRGEGGI"
BASE COUNT         1549 a         3982 c         3814 g         1655 t
ORIGIN      
        1 gcacgagggt ccgccggcag acgcggcagt agcgggcgag gatcttcccc tccttgagcc
       61 cgcggaggta ctcgctgatc gcgatcccgg tgtcccaggc gtagcgcgcg tcgacctggt
      121 ggtccaccgc cccgatcgcc ttcttgaagt cgtcgtcctt gaggtaggtc gggccgagga
      181 agacgtccgg cttcgtcacg tggggctcct cccgcctccg cgccgtcact tgcccatcac
      241 caccacggtg ccgacctgca tcaggtcgcc ccacgcctgc gcgacgccct tgcgcgggtc
      301 gcccggaacc tgccgcttgc cggcctgccc cgagagctgc cagtagagct cagcgatctt
      361 catgagccct gccgcggcga tcgggttccc gacgccgagc aacccgcccg agggcgtcga
      421 ggggagatcg ccgtcgcggt tcagcacgcc gtccttcatc gcctccggcg ccttcccctt
      481 gtcgaagagc atcagcccct cgaggtggtg cagctccttg tagtcgaagg ggtcgtaggc
      541 ctccgcgatg tggatctcct tccgcggctc gcggatgccg gccatgtcgt acgcctggcg
      601 tgcggcgtac tccacgtagc gcgggtagta gaggtcgcgg ttcgtccagt aggcggtgtc
      661 gaggtgccag ccgacgccgt cgatccacac cggccggttc gtgaggcgct tcgccttgtg
      721 ctcggcggcg aggacgatcg cgaccgcgcc gtcggagacc ggcgagacgt cgagacgctg
      781 gaccggccac gcgagcgtct cggacttcag gacgtcgtcc acggtgatgt tcgggtcgcc
      841 gaggagcgcc gcggggtgat ccgccgcgtt gcgcttgttc ttgaccgaca ccgcggcgat
      901 gtccttcttc tcgagaccgt ggaccgtcat gtagcggttc atctcgagcg cgaagatcca
      961 cagcaggttc gggccgagcg gccgctcgag gatgttgtcg aagatcgtga tgaaggcgcc
     1021 ctgcgcgtgc ggctggaccg aggacatctt ctcctcggcg acgacgaggc aggtgtcgaa
     1081 catcccgctc gcgacctggt accagccgtg gatcgtgacg aagacgccgg tgccgccgcc
     1141 gacgaacgtc cggaagtagg gcttgcccca cgcgccggcg ccgtcggcga ggtactcgcc
     1201 cttcatgtgc acgccgtcga acgcgtccgg agccgatccc atcgcgacgc agtcgatgtc
     1261 gtcgagcgtc atctcggcgg actcgagcgc cgccttcgct gcctgccagg cgagctcctt
     1321 gcccgtctcc tgggcccggc ggacgaactt cgtcatcccc gccccgacga ccgcgacccg
     1381 ctgccccatc taggaacccc tcctggcgta ctcgatcatc tggacgcggt tcccatccgg
     1441 atccaccagg ctggcgaacc gcgcgtagcc ctcgacctcg ccgaggtggt cgtcgagcgg
     1501 aaccccgcgc ccggccaggt cggcccgcgc ggcgtcgagg tcctcgacgc ggaagaccgc
     1561 cgtcgccccg ccggtctcgg tccgacgacc ctcgatcgcc ccgtgcaggg ccaggtgcac
     1621 gtcactgccg gtgtcgaaca tcgcccacga gccgccctca cgccgcagga gccgaagccc
     1681 gagggttccc tcgtagaagg agacggcgcg gtccatgtcc tcggtccagt agtagacgtg
     1741 gtcgaggggc ccgagcttca cgcgaccacc tcgtcggcgg cgtagacggc gacggcggcg
     1801 ctcgacgtcg ggacgccgcg ccaggactgg gcgatcgcga cgtcggcgtc ctcgagctgt
     1861 cgctcgcccg cctccccgcg gagctgcagg acgcactcga aggtgcgcgc aagaccgttc
     1921 gcctcgaaga ggttcccgac cccgagcgat ccgccggaga cgttcaccgg caggtcgccg
     1981 tcggccgcga aggcgccctc ctcgagcatc gggccggcct cgccctgccg cgcgagtccg
     2041 agcgcctcga ggctcatgag ctcgcggtac gagtagaggt cgtcgacctc ggcgaggtcg
     2101 acctcgttcg cggggttcga aaggcccgcc atgcggtacg cgcgcttcgc ggcgcgcttc
     2161 acgtagtcgg cctcctccca cgagcggctc tccaggctcg ggctgcccgt gtcccagccg
     2221 atcccgacga cccagaccgg ggagtcggag agatcgcgcg cgacgtcgcc gtcggcgagc
     2281 accgcgacga tgcacccgtc cgcgcgctga gcggcgtcga ggcggcggag cggccaccag
     2341 gtgggatcgg acccgagcac gtcctccgcc gagaggtccg ccgggtgcgc cgcgtgcggg
     2401 ttgtcgaggg cgttgcggcg gttcttctcg acgaccaggg cgcactgctc gtcggtctgg
     2461 cccgtcgcct cgaggtagcg gttcatctcg agcccggcga ccgcgagcgg cgagacgtcc
     2521 agcggccggt tgtagaccgg atccatcgcg aactggtgga tcgacccgag cgagaggatg
     2581 tcggaggcct tcgagtggcc ctcgacagcg accaccttcg ccgcgcccga gcggatcagc
     2641 atcacggcgg tcgcgatcgc gtacaggccg tcggcggtca ccgtctggac cgggcgcagg
     2701 gccgcaccga tctggtcggg gacgtactcg tcgaagatcg aggtcccttc ccagaagtcc
     2761 tcggccgccg tgacgaactg gtcgacgtcc ttccgagggt cgatgcccgc gtcgtcgtac
     2821 gcgcggacag ccgcctcgaa ggtcagctcc ttgtacgaca ggcccgccgt gatcggcgcg
     2881 aagccggcgt gcccgattcc cacgatcgca acccggttcc ccatgagcgg ccggagtcta
     2941 gcaaaggccc gtcggccggc acgaatccgt cacccgacgt gggcgatctc gccgaaggag
     3001 accgtccggc cgtcgacgac gaggccgccg cggtcgtcga tgcgctcggc caggccttcc
     3061 acgacccgcc cgtcgacggt ggtggcgcgg acccggcgac cgatcgtgtc gcagatctcg
     3121 cggtaggcgg agacgatgcg gcggccgtgg ccggcggacc ccggcgccca ggcggcgcgg
     3181 aagccgtgga ggaagccggc gagcagctcg agcggctcgg ccgggctccc ctcgatcgcg
     3241 agactcgtgg ccacctcggc gagctccgct gggaagtcct cctcggcctg ggccacgttc
     3301 acgccgacgc ccaggaacag gacctcgagc tcctccccct ggacgcgggc ctcggtgagg
     3361 atcccgccga ccttccggcg gccggcgagg aggtcgttcg gccacttgga ccggacggcg
     3421 accccggcca cctcccgaca cgcccgcgcc atctcggcgg cggccagcag gctcgtcagc
     3481 gaggcctcgg cggggtggag gggtgggcgg agcaggagcg tgaactgcag gagcttcccg
     3541 gggaccgagt gccaggtcct ccgctcgcgt ccgcggcccg ccgtctggtg cccggtcgcg
     3601 atcacggtcc acgagggcgc accgcgcgcg gcgaggacga gggcctcgtc gttcgtcgaa
     3661 gccgtctcgt cgaaccagac cggtggagcg tcgatcccgg cgcgctggac cgcctgctcc
     3721 aggctatgct ggctcacgcc ccgaagccta tccaacggga gcgagcacat ggctgagaca
     3781 cgggtgctga tcgcgaaccg cggcgagatc gccgtgcgcg tcatccggac gtgccgcgag
     3841 ctcggcatcc caaccatcgc cgtgtactcc gacgccgacc gcgacggcct gcacgtggag
     3901 atggccgacg aggcctaccg catcggcccg gcgaccccgt cgcagtcgta cctgaacgtc
     3961 gccgcgatcc tcgacgcagc cgcccgtggg cgcgcgacgc acgtgcaccc tggctacggg
     4021 ttcctctccg agaaccccga cttcgcccgc gcgtgcgccg acgcgggcct cacgttcatc
     4081 gggccgccgc cggaggcgat ggagatgatg ggcgacaagg cggccgctcg acgcgcagcc
     4141 gagtccgtcg gggtgccggt gatccccggc gtctccgagc cggtgagcct cgagcaggcg
     4201 cgcgaggtgg cctcgtcgat cggctacccg atggccgtga aggccgcgtt cgggggcggc
     4261 ggccgcggga tgcacctgat cgaggacgcc tccgccctcg acgagtccct cgagcggtcc
     4321 gcgcgcgagg cgaaggcgta cttcgggcgc cccgaggtct acctcgagcg ctacatcgcg
     4381 cgcgcgcacc acgtcgaggc gcagatcatc gccgacacgc aaggcaacat ctccttcctc
     4441 ggcgagcgcg actgcacgct gcagcgccgg taccagaagc tgctcgagga gagcccctcg
     4501 ccggtcgtcg atgagagcct gcggacacgg atcggcgagg catcactcgc gatcgcgaag
     4561 gccgcgggct acgcgaacgc cgggacggtc gagttcctcg tcgacgacga cggcgccttc
     4621 tacttcctcg aagtgaacgc gcggctccag gtcgagcacc ccgtcaccga gatggtcacg
     4681 gggctcgacc tcgtgaagct ccagctcgcg gtcgcgcacg gcgagaaggt cgacctgacc
     4741 ccggacctgc gcgggcacgc gatcgaatgc cgcatcaacg cggaggaccc gacgaaggac
     4801 ttcctgcccg ggcccggact gctgacgcgg ttccgccccc ccggggggcc gttcgtgcgg
     4861 ctcgacaccg ggttcggcga ggggaaggtc gtaccgggcg attacgactc cctattcgcg
     4921 aagctcctcg tctggggtga ggaccgcgag gctgcgcgcc ggcggatgct gcgggccttc
     4981 gacgagttcg agatcgaggg cgtccccacg acgatcgagt tctaccgctg ggcgatggag
     5041 accctcacgt tccgggaggc gagccacacg acgaaatggg tcgagggcgc gctggccgag
     5101 ggccagttcc agccggcggg gccggaggcg gccgcgacgg cggcgccgcc ggttcggccg
     5161 gcgagggtca tcgtcgagct cgacggccgc cgcgtccccg tgcaggtctg gggcgacgac
     5221 ctcccggcgc cgcccgagcc gcccgccgcc gtgtccgggg gcggcggggc gcacggccac
     5281 gacgtcctcg tcgccccgat gcagggcacg atcctcaagg tcctcgtcga ggagggccag
     5341 gagatcgcgg ccggcgactc cgtctgcatc ctcgaggcga tgaagatgga gaaccacatc
     5401 gcctccaccc gtgccggcgt gatcgagaag gtcgccgtca agcccggtga cgtggtcgac
     5461 ggcggccagc tcctcgtcgg tttcgcggac tagctagcgg cgtttgcggg ctttccagac
     5521 ctcttcgacg ggccacttgc gtgcccagtc gagctcggcc caggggtagc gcgtcgtgga
     5581 gccctcgggt gggcggacct cgatcaggtc ctcgatctcg aagccggtct cgcgcaggag
     5641 ccgcacccag tcgccgtggc cgaggtggaa ctcgacgctt tcgtcgtcct cccactcgaa
     5701 gcggtgcatc ccgaagtacg gacgcaggag gcggtcggat gccgggccat ccgtcccgag
     5761 atccgggatc gtcagcgcca tcaggacccc catggtcagg aagatcaagg atccgccggg
     5821 ccgcaggatg cgcgccgcct ccgggatcca gcggtaggga tcgcaccaca gcgccgcgcc
     5881 gtactcgttg atcacgaggt cgaaggcagc gtccgcgaac ggcgggtgct cggcatcgcc
     5941 gtgaaccagg gggaagcgaa ggccgtgctc ccgctggagc cgtgccgccg tctcgagctg
     6001 gcgctccgcc aggtcgagcc ccacgggcct cgctccccgg cgcgcgagcc aggcggagac
     6061 gtacgccgtc ccgcacccga gctccgcgac ccgcagcccg tcgaggtcgt ccggcagcat
     6121 ccggacctcg ctctccgggg cggagaagac cccccagtac ggcccgtccg aggcccacgc
     6181 cgcttccgcg gcttcgacgt agttccccgc gtcggcgttc cagtccgcgc ggttgcgccg
     6241 gatgtggtcg ggttctcgtt gcctcagagc gccgcctccg cgaggaggtc gacctgcttg
     6301 aggtcggcga ccgtcgggtc gaagacgagc tcgtcgacgc cggcgtcctc gaacatcttc
     6361 gcggtggtgc ggatgtcctc cggggacttc ggcatgtact cgaagatctt cggcccgaag
     6421 tcgccgtagt agtcggtgag gctggcgagg ccctcctgtt cggcgcctcc gagcgcgtag
     6481 taggcaagcg ccatgatctt cggctcaccc tcccgccccg cctccttcca ggcggcgcgc
     6541 acacgctcgg cgaaggggcc gacctgctgg ggggcggatc cgccggcggt ccacccgatc
     6601 ccccagcgga ccgtgcgccg gatcgccgcg tccgagcttc cgccgacgat gatcgggacc
     6661 gtcccggcga tcggcgcggg cgtcacgggc ttgcgggcgc cctccacgag ctcaccggcc
     6721 caggcacgat ggatcacctc gagcgaacgg tcggcgcgct tcccccgctc ctcgaagggg
     6781 acgcccgccg cctggtagtc gtcgtcgcgc gtccccaccc cgatgcccat cgtgaagcgg
     6841 ccgccggaga tctggtcgac gctcgccgcg gccttcgcga tcatcacggg gtcgtgcgtc
     6901 gggatcagca ggacgttcga gacgaggccg atccgctcgg tcacggcgcc ggcagccgcg
     6961 agcgtcgcca tcgtgtcgta cgacgggtag gcgacccgtc cgatcgtggc cagcgaggag
     7021 aacccgagat cctcggcgtg gcgcgcccac tcgaccagca tccgccccgg cgtgccgggg
     7081 atcgtgttcg gcagtccgat cccgatcttc atgcgtcgct ccttccccat cgctcgcggc
     7141 cggtcagtcg gatcccggtg tccctgagac cgtaggtctt gttcacccgg atcatcaccg
     7201 ccgtgagcgg ctcgaggatc cgggccatcg tgagcggccc gcagtcgacc caccgcatcc
     7261 cgatcttctc gacgaggccc ccgaccgcgg ccttcgcctc cccgtcgccg cacaggagga
     7321 cgtcaccctg gaggtcgccg tcgagcgcct cgagcgcctc cgcgccgacc gagtggaacc
     7381 cggaggcgag gcgcaccccg ggcggcagga gcgccttcgc ctgctcggcg gccgagccct
     7441 gccacgggcg gaggacctgc gtcgcggcgc cgccgacagc cgttgcgagc ggcgtcgtcg
     7501 tgtcgcacac gatcgcgccg tcgcgccacg cctccttcag cgcgcggtag gtggccgcct
     7561 gaccttcgaa cggaacggtc acgaaggtca catcaccggc ggccaccgcc tccgggttgg
     7621 ctcgcccgtc gaccggcgcg tcaggaccga ggacctcctg ggcccgggcg acggcatcgc
     7681 ccgccttctc ggcagagcgg gatccgatca cgacctcctg gcccgccgca gccaggcgca
     7741 gcgcgaggcc gaaaccgagg tccccggtcc ccccgacgat cgcgaccgtc atgccggccg
     7801 gtgctcgccg aagacgcgcc gcagggcgtc ggaggcctcg ccgagcgtcg cccgcgcctt
     7861 gagggcctcg cgcatcggcg gcaagaggtt gtccgtgccg cgcgcggtct cctcgacctc
     7921 cttcagcgcg cgctcgacct cggcgttgtc gcgccccgcg cggatctcgc gcaggcgctc
     7981 cgactgccgc cgcacctgct cttcgtcgag ccgctggagc tcgacgggct cctcgtcctc
     8041 gctgacgaag cggttcacgc cgacgacgac acgctccccc gactcgacgc cctgctggat
     8101 gcggaaggcc gcctcatgga tctcgtcctg gatccagccg cgctcgatcg ccgggaccga
     8161 gccacccatc gcgtcgatct tctcgatgta gtcccaggcg cccgcctcga tctcgtcggt
     8221 tagcgcctcg acgaagtagg acccgccgag ggggtcgacc gtgtcgatca cgccggtctc
     8281 gtacgcgatc acctgctggg tccgcagcgc cacgcgcgcg gccgtctcac tcgggagcgc
     8341 gagcgcctcg tcgaaggagt tcgtgtggag cgactgcgtc ccgccgaaga ccgcggcgag
     8401 cgcctcgagg gccgtgcgca ccacgttgtt ctcgacctgc tgagcggtga gcgtcgcccc
     8461 gccggtctgc gtgtggaagc ggagcatcag cgacttcgcg tccttcgcgc cgaaccgctc
     8521 cgacatgatc ttcgcccaca tgcggcgcgc ggcgcggaac ttcgccacct cctcgaagaa
     8581 gttcatgtgg caggcgaaga agaacgagag tcgcggcccg aagtcgtcga ccttcaaccc
     8641 ggcgtcgagc gcggcctgca cgtacgcgat cgcgttcgcg agcgtgaacg ccacctcctg
     8701 cacggcggtc gagcccgcct cgcgcatgtg gtagccggag atcgagatcg tgttccaccg
     8761 cgggatccgc tcgcggcagt aggcgaacag gtcggtgatg atccgcatcg acggcttcgg
     8821 cgggtagatg taggtcccgc gggccgcgta ctccttgagg atgtcgttct ggacggtccc
     8881 gccgagcttc tcgggaccga tgccctgctc ctccgccgtc agctcgtaga gcagcagcag
     8941 gatcgcggcg gtcgcgttga tcgtcatcga ggtcgtgacc tggtccagcg ggatctcggc
     9001 gaagagcgtc ttcatgtcct cgatcgagtc gatcgccacg ccggtccggc ccacctcccc
     9061 ctccgcgcgg gggtggtccg agtcgtagcc catctgcgtg ggaaggtcga aggcgaccga
     9121 gagcccggtc tgcccgtggt cgaggaggta ccggaaccgc cggttcgtct cctcggccgt
     9181 ccccatcccc gcgtactgcc gcatcgtcca cgggcgtccg cggtacatcg agggatagat
     9241 cccacgcgtg aagggcggct cgccgggccg gccgagctcc ggcgggcggt ccggcgcttc
     9301 gtacagcggc cggatctcga tccccgagtc ggtgcggcgc tcgtcctcgg ccatgcccag
     9361 agcgtacccg ctcaccggtt tcgaaggggg tgagggccgg tgctagactc gcccgcggca
     9421 tggcgaagcg gacctcggag ctcgggggga tctacaaggc cgggaccgga cagtgggcct
     9481 ggctcgccca tcgcatcacc ggggtcggga tcctcttctt cctcttcgcc cacatcgtcg
     9541 acacggcgct cgtcgcgtgg gggccggaga cgtacaacaa ggtcatcgcc ctctaccacc
     9601 acccgatcgt gcagatcatg gagatcgggc tcgtgggcgc ggtcatcttc cacggcgtga
     9661 acgggatccg gatcatgatc atggacttct ggccgaagtc gctcctgtac caccggcagc
     9721 tcacgtgggc gaccctgctg gtcttcttcg ccgcgatggg gccggtcaca tggatcatgg
     9781 gcgaccgcgt gctccacgcg ctggggtggc tctgatggcc gtcgcgaccc gcgagccgac
     9841 cctgcccgcg gagccgcgca cgcgccgccg cgggttcgag ctctggtcct ggctgttcat
     9901 gcggatcagc gggatcctgc tgctgttcct cgccgtgggc cacgtcctga tcatgcacgt
     9961 gatggaggag ggcgtggagc gcgtgaactt cgacttcgtc gccgggcgct gggcgaaccc
    10021 gctgtggaag acctgggact gggcgatgct ctccctcgcg ctcctccacg gcctgaacgg
    10081 gatgcgcgtg atcatccagg actacgtgcg tcggccgggc tggcgcctgt tctggaacag
    10141 cacgttctgg gtcctcggct tcggactgtt cctgctcggc acgatcatcg tgctgacctt
    10201 cgacccgacg gtcttctgac gtgacgatcg agcacacgta cgacgcggtg atcgtcggcg
    10261 cgggcggagc gggcctccgg gccgctatcg aggccgccgg ctccgtcaag acggccgtga
    10321 tctcgaagct ctacccaacc cggtcgcaca ccggagccgc gcagggcggg atgtgcgcgg
    10381 cgctcgggaa cgtcgaggag gactacccgg agtggcacgc gttcgacacg gtgaagggcg
    10441 gcgactacct cgtggaccag ccggcggccg agatcatggc gaacgaggcc gtcgacgctg
    10501 tgatccagct cgagcactgg ggcttcccct tcaaccgcac gcccgacggc aggatcgacc
    10561 agcggcggtt cggaggccac acgcgcaacc acggcgaggc cgcggtccgc cgggcctgct
    10621 acgcggccga ccgcactggc cacatgatcc tccagaccct ctaccagcag tgcatcaagc
    10681 gcgacgtccg cttcttcaac gagttctacg tgctcgacct cctcatgcag gacgggcgcg
    10741 cggccggtgt cgtcgcctac gagctctcga gcggcgagct ccacgtgttc cacgcgccgg
    10801 cggtgctctt cgccacgggc ggctacggca agatgttcaa ggtctcttcg aacgcccaca
    10861 cgctcacggg cgacggcccg gcggtctgct tccggcgcgg gatcccgctc gaggacatgg
    10921 agttcttcca gttccacccc accggcatct acaagatggg catcctgatc tccgaggcgg
    10981 tccgcggcga gggcgggatc
//
LOCUS       DATLGG010000173         4634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_70290, whole genome shotgun sequence.
ACCESSION   DATLGG010000173 DATLGG010000000
VERSION     DATLGG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70290"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VM638_04930"
     CDS             <1..339
                     /locus_tag="VM638_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM11801.1"
                     /translation="RHTPADTPIWVRVSATERDVLIVVEDAGPGVPEELRTAIFRPFE
                     QGTRGDGHSPGVGIGLSIVGRFAELHGGRAWVEERPGGGASFRVTLPLDGSERERPRT
                     RSPADRTARA"
     gene            complement(359..1246)
                     /gene="pdxS"
                     /locus_tag="VM638_04935"
     CDS             complement(359..1246)
                     /gene="pdxS"
                     /locus_tag="VM638_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HVM11802.1"
                     /translation="MERETGTFRVKSGLAEMLKGGVIMDVTSAEQAKIAEEAGAAAVM
                     ALERVPADIRAEGGVARMADPTKVQEIMDAVTIPVMAKCRIGHFVEAQILQALGADYI
                     DESEVLTPADEAHHIDKWGFDVPFVCGARDLGEALRRIAEGAAMIRTKGEAGTGNVVE
                     AVRHVRTINTQIARLRGLRPEELGAVAKELNAPLELLRQVAERGSLPVVNFAAGGLAT
                     PADAALMMQLGVDGVFVGSGIFKSKDPAARAAAIVEATTSYNDPEVLAKVSAGLGEPM
                     KGLEISTLGEDGLLQTRGW"
     gene            complement(1304..2329)
                     /locus_tag="VM638_04940"
     CDS             complement(1304..2329)
                     /locus_tag="VM638_04940"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HVM11803.1"
                     /translation="MTSRGKVVLGTVLTVLAIGGAAFGYMALTGKDVPIVGNLIRPDP
                     PTCPLTGEPPSDEALLDRPVLAVKVENIAESRPQAGLARADVVYEQPVEGGITRFIAL
                     YHCRDADRIGPVRSGRRMDADVLRQFGRPIFAFAGGVDAVFRSVARARLVDMNFIVAE
                     QLYGKDPNRLAPHNLFISTSSFYEAAEGGEAPGETPFSFADEPPGETASKPARVIRAT
                     FSPQAEVLWRYNRKRDRWQRFHGEERHRLESGAVSARNVVVQVVNVRAGGVLDAAGNP
                     SPEVDVTGRGKVYVFRDGRVVEGRWSRPRRGALTEYVDANGDPIPLAPGTTWVELFPR
                     DLPFSFE"
     gene            complement(2390..3286)
                     /locus_tag="VM638_04945"
     CDS             complement(2390..3286)
                     /locus_tag="VM638_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVM11804.1"
                     /translation="MYEQISRNRRRSILLVLLVLALAVGIGYLFGELFAIGPAGAVVA
                     LVIALAMSLSSYFFGDRIVLRVSKARPATRETHPRLINIVEGLCIASGLPMPKVYVVP
                     EEAPNAFATGRTPETASIAVTEGLLRDLDRLELEGVIAHELSHVKNRDILVGTLVATL
                     VGVVVLLADWMRRALFWGGRSTGRGRGWPVGAALFAVGMLLSILAPLGAQLIRFAVSR
                     RREYLADADGALMTRYPPGLASALRKIANAPNRMRVTNNATAHLWFSQPSRIEGPGVS
                     WFERMFSTHPPVEERVRALEEM"
     gene            complement(3288..3839)
                     /locus_tag="VM638_04950"
     CDS             complement(3288..3839)
                     /locus_tag="VM638_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVM11805.1"
                     /translation="MTWIWIVVLVPAVLLLYGIVAFNRLVRHRNRVNSSWAGIDVQLR
                     RRYDLIPNLVEAVQGYAAHERQLFERVTEARTRAIQAGTVHDQADAETDVTRNLRQLM
                     AVAEAYPQLRANENFLALQEELTGTESKIAFARQFYNDQVMRYNTLIQQFPSLVVARI
                     ANFTPREFFEAEEASRGPVGVNP"
     gene            3925..>4634
                     /locus_tag="VM638_04955"
     CDS             3925..>4634
                     /locus_tag="VM638_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HVM11806.1"
                     /translation="MPRLILLCLLVLLPACQGASDPGRPTVVAAVYPLAYVAERIGGP
                     AVEVIDLTPAGSDAHDVELSLEDRAAIEEADVVVYMGQLGFQPQVEAAVADAAGHVVD
                     ASRGDQTLAAGEDPHRWLDPIFVRDAAEDLAEAIASADPARADAHRQRAARLVADLDR
                     LADEVGVEDCSHHTAIVTHEAFASLLPEPFRQHGLAGADPEGEPTSDRLREAARLVRA
                     GDAGAVFYEETDEGRRVGT"
BASE COUNT          660 a         1664 c         1649 g          661 t
ORIGIN      
        1 cggcacaccc ctgcggacac gccgatctgg gtccgggtct cggcgacgga gcgcgacgtg
       61 ctgatcgtgg tcgaggacgc cggccccggc gtgcccgagg agctgcggac ggccatcttc
      121 cgccctttcg aacaggggac gcgtggcgac gggcacagcc ctggggtcgg gatcgggctc
      181 tcgatcgtcg ggcggttcgc cgagctgcat gggggtcgcg cctgggtcga ggagcgtccc
      241 ggcgggggcg cctcgttccg cgtcacgctc ccgctcgacg gttcggagcg cgagagaccc
      301 cgcacccgaa gcccggccga tcgaaccgcc cgcgcctgac ctgggcccgg ggaccgatct
      361 accagccgcg ggtctgcagg agcccgtcct cgccgagcgt cgagatctcg aggcccttca
      421 tcggctcgcc gaggccggcc gagaccttcg ccaggacctc ggggtcgttg taggacgtgg
      481 tcgcctccac gatcgcggcg gcgcgcgcgg cggggtcctt cgacttgaag atccccgacc
      541 cgacgaagac gccgtcgaca ccgagctgca tcatcagcgc cgcgtcggcc ggcgtcgcga
      601 gacctccggc ggcgaagttc accacgggga gcgagccgcg ctccgcgacc tgccggagga
      661 gctcgagcgg cgcgttcagc tccttcgcga ccgcccccag ctcctcgggt cgcaggccgc
      721 gcagccgtgc gatctgcgtg ttgatcgtcc gcacgtgtcg cacggcctcc acgacgttgc
      781 cggtgcccgc ctcccccttc gtgcggatca tcgccgcgcc ctcggcgatc cggcgcagcg
      841 cctcgccgag gtcgcgcgcg ccgcagacga acgggacgtc gaacccccac ttgtcgatgt
      901 gatgcgcctc gtcggcgggg gtcagaacct cggactcgtc gatgtagtcg gcgccgagcg
      961 cctgcaggat ctgcgcctcg acgaagtgtc cgatccggca cttcgccatg accgggatcg
     1021 tcacggcgtc catgatctcc tggaccttgg tgggatcggc catcctcgcc acgcctcctt
     1081 cggcgcggat gtccgcggga acgcgctcga gcgccatgac ggcggcggcg ccggcctcct
     1141 cggcgatctt cgcctgctcg gcggaggtga cgtccatgat cacgccaccc ttcagcatct
     1201 cggccagccc ggacttcacc cggaaggtgc ccgtctcgcg ttccatcctg cctccctcga
     1261 acggtcctgc cgctcaggat acgcccgatg ggcgcagcgg gacctactcg aaggagaacg
     1321 ggaggtcgcg cgggaagagc tcgacccagg tcgtgccggg cgcgagcggg atcgggtccc
     1381 cgttcgcgtc cacgtactcg gtgagggcgc cacgccgggg ccggctccat cgcccctcga
     1441 cgacacggcc gtcgcggaag acgtagacct tccctcgccc cgtcacgtcg acctccgggg
     1501 aggggttgcc cgcagcatcg aggacgccgc ccgcccgaac gttgacgacc tggacgacga
     1561 cgttgcgagc cgacacggcg ccggactcga gccggtggcg ctcctcgccg tggaaccgct
     1621 gccaccgatc gcgtttgcgg ttgtaccgcc agaggacctc ggcctgcggg gagaaggtcg
     1681 cgcggatcac gcgggcgggc ttcgacgcgg tctcccccgg gggctcgtcc gcgaaggaga
     1741 acggcgtctc cccgggtgcc tccccccctt cggctgcctc gtagaagctc gacgtcgaga
     1801 tgaacaggtt gtgcggcgcg agccggttcg gatccttccc gtacagctgc tccgcgacga
     1861 tgaagttcat gtcgacgagc ctcgcgcgcg cgacggagcg gaagacggca tccacgccac
     1921 cggcgaacgc gaagatcgga cggccgaact gccggagcac gtcggcgtcc atccgacggc
     1981 cgctgcgcac cgggccgatc cgatcggcgt cgcggcagtg gtagagcgcg atgaacctcg
     2041 tgatgccgcc ctcgaccggc tgctcgtaca cgacgtccgc gcgggcgagg cccgcctgcg
     2101 gccgagactc ggcgatgttc tcgaccttca ccgcgaggac cggccggtcg agcagcgcct
     2161 cgtcgctcgg cggctccccg gtcaggggac acgtcggggg atcgggtcgg atcaggttcc
     2221 cgacgatcgg gacgtccttc ccggtcagcg ccatgtagcc gaacgcggcg ccgccgatcg
     2281 cgagcaccgt caggacggtg cccagcacga ccttgcctcg ggatgtcacg gttggggggg
     2341 acctcctcgc tctcgctccg atgctagccg cgcccggggg gcgcttcaat cacatctctt
     2401 cgagcgcgcg gacccgctcc tccacgggcg gatgcgtgga gaacatccgc tcgaaccagc
     2461 tgacacccgg tccttcgatc cgtgaaggct gcgagaacca caggtgcgcc gtggcgttgt
     2521 tcgtcacgcg catgcggttc ggcgcgttcg cgatcttccg cagggccgag gcgagcccgg
     2581 gcgggtaccg cgtcatgagc gcgccgtccg cgtcggcgag gtactcgcgg cgacgggaga
     2641 cggcgaagcg gatcagctgg gcgccgagcg gggcgaggat cgacagcagc atcccgacgg
     2701 cgaacagcgc cgccccgacc ggccagccgc gtccgcgccc ggtgctccga ccaccccaga
     2761 agagggcgcg gcgcatccaa tccgccagca gcacgacgac cccgacgagg gtcgcgacca
     2821 gggtccccac caggatgtct cggttcttca cgtgcgagag ctcgtgcgcg atgacgccct
     2881 cgagctcgag gcggtcgagg tcgcgcagga gcccctcggt gacggcgatc gaggccgtct
     2941 cgggcgtccg ccccgtcgcg aacgcgttcg gcgcctcctc cgggacgacg tagaccttgg
     3001 gcatcggcag gccggaggcg atgcaaaggc cctcgacgat gttgatcagg cgtgggtggg
     3061 tctcacgggt ggcgggtcgt gccttgctca cccgcaggac gatgcgatcg ccgaagaagt
     3121 acgaggacag cgacatcgcg agggcgatga cgagggcgac aaccgcgccc gccgggccga
     3181 tggcgaacag ctcgccgaag aggtagccga tcccaacggc gagcgcgagg acgagcagga
     3241 ccaggaggat ggaccggcgg cggttgcggg agatctgctc gtacaactca gggattcacg
     3301 ccgacgggcc cgcgcgacgc ctcctcggcc tcgaagaact cccggggggt gaagttggcg
     3361 atccgggcga cgacgagcga cgggaactgc tggatgaggg tgttgtagcg catcacctgg
     3421 tcgttgtaga actgccgcgc gaacgcgatc ttgctctcgg tgccggtcag ctcctcctgc
     3481 agggcgagga agttctcgtt cgcgcggagc tgcgggtagg cctcggcgac ggccatcagc
     3541 tggcgaaggt tcctcgtgac gtcggtctct gcgtcggcct ggtcgtggac cgtcccggcc
     3601 tggatcgccc gcgtccgcgc ctccgtcacg cgctcgaaca gctgccgctc gtgcgcggcg
     3661 taaccctgga ccgcctcgac gaggttcggg atcaggtcgt accggcggcg gagctggacg
     3721 tcgatcccgg cccaggagct gttcacgcgg ttgcggtgcc gcacgagccg gttgaacgcc
     3781 acgatcccgt agagcagcag caccgcgggc accagcacga cgatccagat ccacgtcacg
     3841 gcgcggatag taccaggggc gtccggcccg gcctcggcgt gcgcgccttg acgtcgcatc
     3901 catgagactg ataatcattc tcacgtgccg cgtctcatcc tgctctgcct cctcgtcctc
     3961 ctccccgcct gtcagggcgc gagcgatccc ggtcgcccga ccgtggtggc ggccgtctac
     4021 ccgctcgcct acgtggccga gcggatcggc gggcccgcgg tcgaggtgat cgacctcacc
     4081 cccgccgggt ccgacgcgca cgacgtggag ctctccctgg aagaccgcgc ggcgatcgag
     4141 gaggccgacg tcgtggtcta catgggccag ctcgggttcc agccgcaggt cgaggccgcg
     4201 gtcgccgacg ccgccgggca cgtcgtcgac gcctcacgcg gcgaccagac gctggccgcg
     4261 ggcgaggacc cccaccggtg gctcgacccg atcttcgtcc gcgacgcggc ggaggacctc
     4321 gccgaggcga tcgcctcggc cgaccccgcg cgggccgacg cccaccggca gcgggccgcg
     4381 cgactcgtgg cggacctgga tcgcctcgcg gacgaggtcg gggtcgagga ctgctcccac
     4441 cacacggcga tcgtgacgca cgaggccttc gcatccctcc ttcccgagcc gttccgccag
     4501 cacggtttgg cgggcgcgga tccggaggga gagcccacgt ccgaccggct ccgggaggcg
     4561 gcccgcctgg ttcgggcggg tgacgccggg gccgtcttct acgaggagac cgacgagggg
     4621 cgtcgcgtcg gaac
//
LOCUS       DATLGG010000174         5443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_211603, whole genome shotgun sequence.
ACCESSION   DATLGG010000174 DATLGG010000000
VERSION     DATLGG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211603"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /gene="tsf"
                     /locus_tag="VM638_04960"
     CDS             <1..283
                     /gene="tsf"
                     /locus_tag="VM638_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708782.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HVM11807.1"
                     /translation="WVSREEVPAEEVDRERRIAEEQAREQGKPENVVPKIVEGKLQSF
                     YKDVCLLEQPFIKDDKLTVGDLVTEAAAKVGENVTIRRFARFQLGDEAS"
     gene            292..1056
                     /gene="pyrH"
                     /locus_tag="VM638_04965"
     CDS             292..1056
                     /gene="pyrH"
                     /locus_tag="VM638_04965"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVM11808.1"
                     /translation="MVPEREAQHGGAGPDGTLYRRVLLKLSGDAFAPDDREYGISADA
                     VALLARQLADVVERGVQTAVVVGGGNIFRGRDAPGMDRARADYMGMLATVMNALALQD
                     GLERIGVTTRVQTAIAMAQIAEPYIPLRGIRHLEQGRIVIFAAGLGRPFFSTDTTAAQ
                     VALEIHAEAILKGTRVDGVYDSDPNLNPDAVRFDHLEYIEVLKRGLQVMDATAISLCM
                     ENELPIIVFDYREEGNIVRALMGEKIGTVVDAGRMR"
     gene            1053..1616
                     /gene="frr"
                     /locus_tag="VM638_04970"
     CDS             1053..1616
                     /gene="frr"
                     /locus_tag="VM638_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVM11809.1"
                     /translation="MSTAPEILKDAVHKMERAVDVSRDEFATVRTGRASPSILNRITV
                     DYYGAPTPLNQLASFSVPEPRLLVIQPFDRNSIAAIEKAIMQSDLGLTPSNDGQLIRL
                     AFPPLTEERRKELTRVVRERAEDGRVAVRNVRRHAKEELERLEKNHEISEDELARAEK
                     ELQKHTDEHVAKIDDLVAHKEKELMEV"
     gene            1619..3337
                     /locus_tag="VM638_04975"
     CDS             1619..3337
                     /locus_tag="VM638_04975"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVM11810.1"
                     /translation="MVGPDDHRDDDGEDLFEDLDRFFAPIKDTDWPEEEPPPPAEAEQ
                     PAEQPDAPPTPAASAEDEDILGGWGGPQIDIPDPEELVGDLFPEPEPEAEPVLLSEPE
                     PGEEPAPEPELPSEPEPEPELPREPEPEPVGGPAAAAEDEGVDEWERFREEVQREAAG
                     TDDATEIGEPLTIGDGEDEPAVAPPRYAGLPGADHEDDDAWFSELTEEPAEPQVVEVD
                     AEGADRVDVEAAAEHFAAGIRETPADVERELLADLDETAVVPGTVRIDPLDTEMGGEP
                     GPSWDEPGRIPEEEPSGGRSLPAAVISGGLLAVAAVALLAIGKAPFAVLAGAVILLGQ
                     VELYSVIRARGIHPASGLGLVIGLLMIIGAYLRGEPALLFMLVLGMGLVIPWYMASPP
                     KARGGMLTNVAMTILGIVYVPFLASFALVLLRLPGPVGRNLLLTVVGLTVLYDICAYA
                     IGSWWGDRPLAPTISPRKSWEGVIGATFALLIVALAVLPLFAPFADFAGPGSGLGAGP
                     AVGLALIIAIAAPLGDLAESALKRDLGIKDMGTILPGHGGILDRVDSLLFALPAAWYF
                     LRVVLL"
     gene            3373..4539
                     /locus_tag="VM638_04980"
     CDS             3373..4539
                     /locus_tag="VM638_04980"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVM11811.1"
                     /translation="MRKVIILGSTGSIGTQALDVVRRNPDRFEVVGLAAAQNQELLVG
                     QIREFMPPVVAVSDETAAAELRASLERIKGLKNIDIVSGPQAAEEIARDLEADVVLNA
                     MVGAVGLAPTLSALQSGKTLALANKESLIVGGELVTDLVKGEPERLVPVDSEHAALAQ
                     CLRGERREDLRRVVITASGGPFRGWTREELAKASVKEALAHPTWKMGPKITIDSATLM
                     NKGLEVIEAHFLFNLDYAEISVVIHPESIVHGIAEFRDGSMMAHLSVPDMRLPIQLAL
                     DYPNRMPSGIQPLDLAAVGKLTFEALDRDAFPSLDLCYRAGRAGSTYPAVLNAANEVA
                     VMAFLEGKIPMTGITEIIERTLDEHDPVSVVSIVTLERADAWARRRAAEIIDAR"
     gene            4565..>5443
                     /locus_tag="VM638_04985"
     CDS             4565..>5443
                     /locus_tag="VM638_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVM11812.1"
                     /translation="MPAWIGIVIFVVAVLAIIFIHELGHFLTAKWFGIKVEEFFIGFG
                     PRVWSFRRGETEYGVKAIPAGGYVRIAGMNPYEEPAPEDQARTFGAKPAWQRAIVLVA
                     GSVTHFILAFLLLAFFFGAIGVPSRFSADVAQVEATLEGRPSPAAQAGLRPGDRFVEV
                     DGRPVQDYDEFVEYTRARPGQEIEVVVERDGRRVPLRLTPVLSEVGGERVGRLGVVLS
                     PGEILERERVGPLGAMGRAAAGIGEISVRSVQAMGQVFSPSGLMRIVRQLFGDEPRQI
                     DRARSICRGSSPKSWRT"
BASE COUNT          818 a         1890 c         1944 g          791 t
ORIGIN      
        1 gtgggtctcc cgtgaggagg tcccggccga ggaggtcgac cgcgagcgga ggatcgccga
       61 ggagcaggcc cgtgagcagg ggaagccgga gaacgtcgtg ccgaagatcg tcgagggcaa
      121 gctccagtcg ttctacaagg acgtctgtct cctcgagcag ccgttcatca aggacgacaa
      181 gctgacggtc ggcgacctgg tcaccgaggc cgcggcgaag gtcggggaga acgtgaccat
      241 ccgccggttc gcgcggttcc agctgggcga cgaggcgagc tgaggaggag cgtggttccc
      301 gagcgggagg cgcagcacgg aggggccggc ccggacggca cgctctaccg ccgcgtcctg
      361 ctgaagctct ctggcgacgc gttcgccccc gacgaccggg agtacgggat ctccgccgac
      421 gccgtggcgc tgctcgcgag gcagctggcc gacgtcgtgg agcgaggcgt tcagaccgcg
      481 gtcgtcgtcg ggggaggcaa catcttccgt ggccgggatg ccccggggat ggaccgcgca
      541 cgcgccgact acatggggat gctggcgacg gtgatgaacg ccctcgccct ccaggacggt
      601 ctcgagcgca tcggcgtcac gacccgggtc cagacggcga tcgcgatggc gcagatcgcc
      661 gagccctaca tcccgctgcg cggcatccgc cacctcgagc agggccggat cgtcatcttc
      721 gccgccgggc tcggacgtcc gttcttctcg accgacacga ccgcggcgca ggtcgccctc
      781 gagatccacg ccgaggcgat cctcaagggc acgcgggtcg acggcgtcta cgactccgac
      841 ccgaacctga accccgacgc ggtgcgcttc gaccacctcg agtacatcga ggtcctcaag
      901 cgcggactcc aggtgatgga cgccacggcg atctccctct gcatggagaa cgagctgccg
      961 atcatcgtgt tcgactaccg cgaggagggg aacatcgtgc gcgcgctgat gggggagaag
     1021 atcggcacgg tcgtcgacgc ggggcggatg cgatgagcac ggcccccgag atcctcaagg
     1081 acgccgtcca caagatggag cgcgcggtcg acgtcagccg cgacgaattc gccacggttc
     1141 gcaccggccg ggcgtcgccg tcgatcctca accgcatcac ggtcgactac tacggcgcgc
     1201 cgacgccgct gaaccagctc gcgtccttct ccgtccccga gccgcgcctg ctcgtgatcc
     1261 agccgttcga ccgcaattcg atcgccgcga tcgagaaggc gatcatgcag tccgacctcg
     1321 ggctcacccc ctcgaacgac ggccagctga tccgtctggc gttcccgccg ctcaccgaag
     1381 agcgacggaa ggagctcacc cgggtcgtgc gcgaacgcgc ggaagacggc cgggtcgccg
     1441 tccgcaacgt ccgccggcac gcgaaggagg agctggagcg tctcgagaag aaccacgaga
     1501 tctccgagga cgagctcgcg cgcgcggaga aggagctcca gaagcacacc gatgagcacg
     1561 tcgccaagat cgacgacctt gtggcacaca aggagaagga gctgatggag gtctgaccgt
     1621 ggtcggaccc gacgatcatc gcgacgacga cggcgaggac ctcttcgagg acctcgacag
     1681 gttcttcgcc cccatcaagg acaccgactg gccggaggag gagccgcccc cgccggctga
     1741 ggcggagcag cctgcggagc agccggacgc gccgccgacg cctgcggcat cggccgaaga
     1801 tgaggacatc ctcggcggct ggggcggtcc ccagatcgac atccccgacc ccgaggagct
     1861 cgtcggcgat ctcttccccg agcccgagcc cgaggcggag cccgtgctcc tatccgagcc
     1921 cgagccgggg gaggagccag caccggagcc cgagctccca tcggaacccg agccggagcc
     1981 cgagctccca cgggagcccg agcccgagcc ggtgggggga ccagccgcgg cagccgagga
     2041 cgagggtgtc gacgaatggg agcgcttccg cgaggaggtc cagcgggaag cggcgggcac
     2101 ggacgacgcg accgagatcg gcgagcccct gacgatcggc gacggcgagg acgagccggc
     2161 ggtcgcgccc ccacggtacg cggggctccc gggggccgac cacgaggacg acgacgcctg
     2221 gttcagcgag ctcaccgaag agcccgccga gccgcaggtc gtcgaggtgg atgcggaggg
     2281 agccgatcgc gtggacgtcg aggcggccgc ggagcatttc gcagccggca tccgcgagac
     2341 ccccgccgac gttgagcggg agctgctggc cgacctcgac gaaaccgcgg tggtccccgg
     2401 gaccgtccgc atcgatccgc tggataccga gatgggcggc gagccggggc cgagctggga
     2461 tgagccgggt cggatccctg aggaggagcc ctcgggcgga cgcagcctcc ccgccgcggt
     2521 gatctcgggt ggcttgctcg ccgtcgcggc cgtcgcgctg ctcgcgatcg gcaaggcccc
     2581 gttcgccgtc ttggccgggg cggtgatcct gctcggacag gtcgagctgt actcggtgat
     2641 ccgcgcgcgc gggatccatc cggcctcagg gctcgggctc gtgatcggcc tgctcatgat
     2701 catcggcgcc tacctccgcg gcgagccggc gctgctgttc atgctcgtgc tcggcatggg
     2761 gctggtgatc ccgtggtaca tggcgtcgcc accgaaggcc cgcgggggca tgctcacgaa
     2821 cgtcgcgatg acgatcctcg ggatcgtcta cgtgcccttc ctcgcttcct tcgcgctcgt
     2881 gctcctgcgg ctcccgggcc ccgtcggccg caacctgctg ctcacggtcg tcggactgac
     2941 ggtgctgtac gacatctgcg cgtacgcgat cgggagctgg tggggcgacc gtccgctcgc
     3001 gccgacgatc agcccgagga agtcgtggga gggggtcatc ggggccacgt tcgccctgct
     3061 gatcgtcgcc ctcgcggtcc tgccgctgtt cgcgccgttc gccgacttcg ccggtccggg
     3121 tagcggcctc ggcgcggggc ccgccgtcgg tctggcgctc atcatcgcga tcgcggctcc
     3181 cctcggcgac ctcgccgagt cggccctcaa gcgcgatctc ggcatcaagg acatggggac
     3241 gatcctcccg ggccacgggg ggatcctcga ccgcgtcgat tccctgctgt tcgcgctgcc
     3301 cgccgcctgg tacttcctcc gcgtcgtgct tctctgactt tgccgttgcg agcgggtacc
     3361 ctgggaagcg cgatgcggaa ggtcatcatc ctcggctcca ccggctccat cggaacccag
     3421 gctctcgacg tcgtccgccg caaccccgac cggttcgagg tggtgggtct cgccgccgcg
     3481 cagaaccagg agctgctcgt cggccagatc cgcgagttca tgccgcccgt ggtcgccgtc
     3541 agcgacgaga ccgccgcggc tgagcttcgg gcctcgctcg agcggatcaa gggtctgaag
     3601 aacatcgaca tcgtctccgg cccgcaggcc gcggaggaga tcgcgcggga cctggaagcc
     3661 gacgtcgtgc tgaacgcgat ggtcggcgcc gtcgggctcg cgccgacgct gtcggcgctg
     3721 cagtccggga agaccctcgc cctcgcgaac aaggaaagcc tcatcgtcgg cggcgagctc
     3781 gtaaccgacc tcgtgaaggg cgaacccgag cggctcgttc ccgtcgactc ggagcatgcc
     3841 gcgctcgcgc agtgcctgcg gggggagcgc cgcgaggatc tccgccgcgt cgtgatcacc
     3901 gcctccggcg ggcccttccg cgggtggacg cgcgaggagc tggcgaaggc gtcggtgaag
     3961 gaggctctgg cccacccgac ctggaagatg gggccgaaga tcacgatcga ctccgccacg
     4021 ctcatgaaca agggcctcga ggtgatcgag gcgcacttcc tgttcaacct cgactacgcg
     4081 gagatctccg tcgtcatcca ccccgagagc atcgtccatg ggatcgcgga gttccgcgac
     4141 ggctcgatga tggcccacct gtcggtgccg gacatgcgcc ttccgatcca gctcgcgctc
     4201 gactacccga accggatgcc gtcggggatc cagccgctcg acctcgccgc cgtcgggaag
     4261 ctgacgttcg aggcgctcga tcgcgacgcc ttcccgagcc tcgacctctg ctaccgtgcc
     4321 ggccgcgcgg gctccaccta ccctgcggtg ctgaacgccg cgaacgaggt cgccgtgatg
     4381 gccttcctcg agggcaagat cccgatgacg ggcatcaccg agatcatcga acggacgctc
     4441 gacgagcacg accccgtctc ggtggtctcc atcgtcacgc tcgagcgagc ggacgcctgg
     4501 gcccgccgcc gcgcggccga gatcatcgac gcccgctgat gccggggcgc ccggcgcgat
     4561 cctgatgccc gcctggatcg gcatcgtcat cttcgtggtc gccgtgctcg cgatcatctt
     4621 catccacgag ctcggccatt tcctcacggc gaagtggttc ggcatcaagg tcgaggagtt
     4681 cttcatcggc ttcgggccgc gggtctggtc gttccgccgc ggcgagacgg agtacggcgt
     4741 gaaggcgatc ccggccggcg gctacgttcg gatcgccggc atgaaccctt acgaggagcc
     4801 ggcgccggag gatcaggcgc gcacgttcgg cgcgaaaccc gcgtggcagc gagccatcgt
     4861 gctcgtggcc ggctccgtca cgcacttcat cctcgcgttc ctgctgctcg cgttcttctt
     4921 cggcgcgatc ggcgtgccga gccggttctc cgccgacgtg gcgcaggtgg aggcgaccct
     4981 cgaggggcga ccctcgccgg cggcgcaggc ggggctgcgc ccgggcgacc gattcgtcga
     5041 ggtggacggg cgtcccgtgc aggactacga cgagttcgtg gagtacacgc gcgcgcggcc
     5101 ggggcaggag atcgaggtcg tcgtcgagcg cgacggccgg cgggttccgc tccggctgac
     5161 gccggtcctg tcggaggtcg gcggcgagcg cgttggccgt ctgggcgtcg tactcagtcc
     5221 cggcgagatc ctcgagcgcg agcgggtggg accgctcggt gcgatggggc gagcggccgc
     5281 cgggatcggc gagatctcgg tccgcagcgt gcaggcgatg gggcaggtgt tcagcccctc
     5341 gggcctgatg cggatcgtcc gccagctctt cggggacgag ccccggcaga tcgaccgggc
     5401 ccggtcgatc tgccggggct cgtccccgaa gagctggcgg acg
//
LOCUS       DATLGG010000175         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_137805, whole genome shotgun sequence.
ACCESSION   DATLGG010000175 DATLGG010000000
VERSION     DATLGG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137805"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VM638_04990"
     CDS             <1..673
                     /locus_tag="VM638_04990"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HVM11813.1"
                     /translation="GASVLRAPAITIESIDGEELDLAIRDVAARGFAWVAFTSPATVE
                     VWSRRAPVLGLGPADLRARVAAVGKATEHALTTWGATVDLVPRVFTTDALGRAFPRGE
                     GRVLLPRADIAPPDLEDTLRQRGWEPVRVDAYRTRPAASLPEEACRALEDGAVDAVTF
                     TSASTVDGFVHAAGALRGPRVACIGPVTARRARERGLPVHAVARPHTAEGLVEAVVRA
                     LRGRP"
     gene            715..1092
                     /locus_tag="VM638_04995"
     CDS             715..1092
                     /locus_tag="VM638_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11814.1"
                     /translation="MHRALVPLLAALFLTGCNLEESVAGLVRDEESAGVTLTVEECGT
                     DDAENPYALVSMVSEEKEWGTVLFNVEMLNEDGVVIGQGSTSFRSVEPGKRYQTKVII
                     SFPSEDPPKEPACEVDLEFASRQ"
     gene            1127..2107
                     /gene="hemB"
                     /locus_tag="VM638_05000"
     CDS             1127..2107
                     /gene="hemB"
                     /locus_tag="VM638_05000"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVM11815.1"
                     /translation="MSFPSERPRRLRRTPALRALVRETDLSTDRLVAPLFVKEGIDEP
                     APIASMPGQRQHTLESLRKEARELAELGILGFVLFGIPARKDPEGSEAWAEEGIAQRG
                     IRALKDELGEDGVVFADLCLDEYTDHGHCGVLTDAGEVDNDATLERYRRIAVAQAAAG
                     ADLVAPSGMMDGQVAAIRSALDDGGFTGTGIMAYAAKFASTFYGPFRDAAECAPRFGD
                     RAAYQMDPANAEEALREIRLDVEEGADIVMVKPALAYLDVIALARHEVSVPVAAYNVS
                     GEYAMLKAAAERGWLDGERAMLEALASIRRAGADLILTYHAREAAELLRR"
     gene            2104..>2301
                     /locus_tag="VM638_05005"
     CDS             2104..>2301
                     /locus_tag="VM638_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVM11816.1"
                     /translation="MTEELFDRARELIPGGVNSPVRAFNAVGGTPLFVERGEGAEIVD
                     AAGRRYVDYVQSWGALLFGHAR"
BASE COUNT          307 a          831 c          850 g          313 t
ORIGIN      
        1 cggcgccagc gtcctgcgcg cgccggcgat cacgatcgag tcgatcgacg gcgaggagct
       61 tgacctggcg atccgcgacg tggcggcgcg gggcttcgcc tgggtcgcgt tcacgagtcc
      121 ggcgaccgtc gaggtgtggt cccggcgcgc gcccgtcctc gggctcggac ccgcggatct
      181 ccgcgcgcgg gtcgccgcgg tgggcaaggc gaccgagcat gcactgacga cgtggggagc
      241 caccgtcgac ctcgtcccgc gggtcttcac gaccgatgcc ctcggccgcg cgttcccgcg
      301 cggtgagggc cgcgtgcttc tgccgcgcgc cgacatcgcg ccgccggacc tcgaggacac
      361 cctgcgccag cgcggctggg agccggtgcg ggtcgacgcc taccgcacga ggccggccgc
      421 ttccctcccc gaggaggcct gtcgggccct cgaggatggc gcggtcgacg ccgtgacgtt
      481 cacctcggcc tcgaccgtcg acggcttcgt ccacgccgcc ggcgctctcc gcggcccccg
      541 cgtcgcctgc atcggccccg tgaccgcgcg acgtgcccgc gagcggggcc ttccggtcca
      601 cgcggtcgcc cgcccgcata cggccgaagg cctggtcgag gccgtggtcc gggcgcttcg
      661 cggccggccg tagtccctgc tagagtcccg ccgaccatca cggagggagg ctcgatgcac
      721 cgcgcgctcg tcccgctgct cgcagcgctg ttcctgaccg gctgcaacct cgaggagagc
      781 gtcgccggcc tcgtccgcga cgaggagagc gccggcgtga cgctcacggt cgaggagtgc
      841 ggaaccgacg acgcggagaa tccctacgcg ctcgtcagca tggtgagcga ggagaaggag
      901 tgggggacgg tcctgttcaa cgtcgagatg ctgaacgagg acggcgtggt catcgggcag
      961 ggctcgacca gcttccgcag cgtcgagccg ggcaagcggt accagacgaa ggtcatcatc
     1021 tccttcccct ccgaggatcc gccgaaggag ccggcctgcg aggtcgacct cgaattcgcc
     1081 tcgcgccagt gaggccgccc ggcggcggac ccgataccat cgaggcatga gcttcccctc
     1141 ggaacggccg cgccgcctgc gccgaacccc ggcgctccgg gccctcgtcc gcgagacgga
     1201 cctgtcgacg gaccgcctcg tcgcgcccct gttcgtgaag gaggggatcg atgagcccgc
     1261 cccgatcgcg tcgatgccgg ggcagcggca gcacacgctc gagtcgctgc ggaaggaggc
     1321 ccgcgagctg gccgagctgg ggatcctcgg attcgtgctg ttcgggatcc cggcgcggaa
     1381 ggaccccgag gggagcgagg cgtgggccga ggagggcatc gcccagcgcg ggatccgcgc
     1441 gctgaaggac gagctcgggg aggacggggt cgtcttcgcc gacctctgcc tcgacgagta
     1501 caccgatcac ggccactgcg gcgtgctgac ggacgcggga gaggtcgaca acgacgccac
     1561 gctcgagcgc taccggcgga tcgccgtcgc acaggcggcc gccggggccg acctcgtcgc
     1621 cccgtccggg atgatggacg gccaggtcgc ggcgatccgc tcggcgctcg acgacggcgg
     1681 cttcaccggg accgggatca tggcctatgc ggcgaagttc gcctcgacct tctacggccc
     1741 gttccgtgac gccgccgagt gcgcccctcg gttcggcgat cgggccgcgt accagatgga
     1801 tccggcgaac gcggaggagg ccctccgcga gatccggctc gacgtcgagg agggcgccga
     1861 catcgtcatg gtgaagcctg cgctcgcgta cctcgacgtc atcgcgctcg cccgccacga
     1921 ggtctccgtc ccggtcgccg cgtacaacgt ttcgggcgag tacgcgatgc tcaaggcggc
     1981 ggccgagcgc ggctggctcg acggcgagcg tgcgatgctc gaggcgctcg cctcgatccg
     2041 tcgcgcgggc gccgacctga tcctcaccta ccacgcgcgg gaagccgccg agctgctccg
     2101 ccggtgaccg aggaactctt cgaccgcgcg cgtgagctga tccccggcgg ggtgaactcg
     2161 cccgtgcgcg ccttcaacgc ggtcggcggg acaccgctgt tcgtcgagcg gggcgaaggg
     2221 gcggagatcg tcgacgcggc cgggcgtcgg tacgtcgact acgtccagtc gtggggggcg
     2281 ctcctgttcg ggcacgcacg c
//
LOCUS       DATLGG010000176         3613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_5627, whole genome shotgun sequence.
ACCESSION   DATLGG010000176 DATLGG010000000
VERSION     DATLGG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5627"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VM638_05010"
     CDS             <1..637
                     /locus_tag="VM638_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM11817.1"
                     /translation="IDREALVDSVYGELALVATGLVPPPIPGYRRDACGPRCSRDLGR
                     ARALVSTLSGDELTLDFSDSAVGQQLASALASQLDEVGLSVRPRPHDDESFATLIRDG
                     RQELFCFVWVGDYPRQQAFLEPLLRSDSPDNHTRLEDARIDRILERARAEPDPTAREE
                     RYRQVERLALQRAPLFPIAWFRSRLAVQDYVGGFVLDPLGGFDVSHLSIGR"
     gene            609..2138
                     /locus_tag="VM638_05015"
     CDS             609..2138
                     /locus_tag="VM638_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM11818.1"
                     /translation="MSRTFRSAASGAVAALLLAACPAAGPPPPEPPPAPAGVVRLAYP
                     LDPPTLNPIRATTPEARDVLRALLPSFHVVTPDLRYRPSLLAEEPVVVVEGERMEVRF
                     RIRDDARWSDGRPITVEDVAFTWQVMVHRRLDAPVSTGFEHLLGVERRGPKAGTLVFE
                     PPFTGWRDLFSAGRFVLPARGRPADVAGWDDGPPVSGGPFVLERWVRGRSIDLARNPR
                     AFVGPPLVQGIRLEIVPDATTALQLFEAGEVDALAPMLGLGWGSRLERLDGVDLSRAT
                     GPTQVHLLLHTGRLSRRRDRQSIAQALDRDRFADVVLRGRDRVAQSVLSPEQPGYLPA
                     WAGYGGGGPVRVGRELRLVSPRAELLDLTARWVQTALSTAGADVDLVAVDPQVFERLV
                     LRERDYELALWVTRSGPGPWLDRWFSSDSGERPSTWEDPTLDALLGRVGLGGPEGASA
                     LETSQQLLAQQAAVIPLFRPEVVMASRGVRGVEANPTADGPLWNAEAWAFVERPTTRR
                     T"
     gene            2172..2261
                     /locus_tag="VM638_05020"
     tRNA            2172..2261
                     /locus_tag="VM638_05020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2207..2209,aa:Leu,seq:gag)
     gene            2325..2534
                     /locus_tag="VM638_05025"
     CDS             2325..2534
                     /locus_tag="VM638_05025"
                     /inference="COORDINATES: protein motif:HMM:NF040017.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HVM11819.1"
                     /translation="MALVGWAVVAAIVLGYQGLALASGPGFPTISDLLGVVTRSLVGR
                     WVLFALWLWLGWHLFVRGWRFFLQG"
     gene            2531..2896
                     /locus_tag="VM638_05030"
     CDS             2531..2896
                     /locus_tag="VM638_05030"
                     /inference="COORDINATES: protein motif:HMM:NF040039.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6256 family protein"
                     /protein_id="HVM11820.1"
                     /translation="MSVDGSLGGGVLGGVVAPLASVYLAFLAMLRLYRRSGRDDRRDR
                     HRAPARPLALLRHLLVTAAGGFGVFLGIVLVFSFVFADQERALREALGGGGFLAALAV
                     VVLALAGWIEGSIASRGSR"
     gene            3072..3356
                     /locus_tag="VM638_05035"
     CDS             3072..3356
                     /locus_tag="VM638_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11821.1"
                     /translation="MPGSRKARDLLRDPRLALHGPTEDPPEPPGPWDGEAKLAGLAVP
                     IERHVAEGPPAEWFRLDIREAVLTWIGDPPDRLLIDSWHPERGLRRRERR"
     gene            complement(3389..>3613)
                     /locus_tag="VM638_05040"
     CDS             complement(3389..>3613)
                     /locus_tag="VM638_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11822.1"
                     /translation="PSYTSSFNRRVELTIDNPTFSGTKTNATVNLATGTFSASMPTTF
                     VQPGTRTIYARAIQGGKVSPVDSVPITVTS"
BASE COUNT          406 a         1325 c         1302 g          580 t
ORIGIN      
        1 gatcgatcgt gaggcgctcg tcgactcggt gtacggcgag ctggcgttgg tggcgaccgg
       61 tctcgtcccc ccgccgatcc ccgggtaccg gcgcgatgcc tgcgggccga ggtgcagccg
      121 agacctgggc cgggcgcgag ccctcgtctc gacgctctcg ggcgacgagc tcaccctgga
      181 cttcagcgac tccgccgtcg gccagcagct cgcctctgcg ctcgcgtcgc agctcgacga
      241 ggtcggcctg agcgtgcgcc cccggccgca cgacgacgag tcgttcgcca ccctgatccg
      301 cgacggtcgc caggagctct tctgcttcgt ctgggtgggt gactacccgc gccagcaggc
      361 gttcctcgag ccgctcctgc gttcggactc gcccgacaac cacacgcgcc tcgaggatgc
      421 gcgcatcgat cggatcctcg agcgagcgcg cgccgagccc gatccgaccg ctcgcgagga
      481 gcgctaccgg caggtcgaac gcctcgccct tcagcgagcc cccctcttcc cgatcgcctg
      541 gttccgctcc cgtctcgccg tccaggacta cgtcggcggg ttcgtgctcg accccctggg
      601 cggcttcgat gtctcgcacc tttcgatcgg ccgctagcgg ggcggtcgcc gccctcctgc
      661 tcgcggcgtg cccggccgcg gggccgccgc cgcccgagcc gccaccggct ccggccggcg
      721 tcgtgcgcct cgcgtacccg ctggacccgc cgaccctcaa cccgatccgc gcgacgaccc
      781 cggaggcgcg ggacgtcctg cgtgcgctgc tgccatcgtt ccacgtcgtc acgccggacc
      841 ttcggtaccg gccctcgctc ctggcggagg agcccgtggt cgtcgtcgag ggggagcgga
      901 tggaggtccg gttccggatc cgggacgacg ccaggtggag cgacggacgc ccgatcacgg
      961 tcgaggacgt tgccttcacc tggcaggtca tggtccatcg gcgcctcgat gcgccggtgt
     1021 cgaccggctt cgagcacctc ctgggcgtcg aacggcgcgg gccgaaggcc gggaccctcg
     1081 tgttcgagcc accgttcacc ggctggcggg acctgttctc cgcggggcgc ttcgtccttc
     1141 ccgcccgcgg acgcccggcc gacgtcgccg gctgggacga cggaccaccg gtgagcggcg
     1201 gcccgttcgt cctcgagcga tgggtccgcg gccgctcgat cgacctggcc cggaacccgc
     1261 gtgccttcgt cgggcccccc ctcgtccagg ggatccgcct ggagatcgtg cccgacgcga
     1321 cgacggccct gcagctgttc gaagcgggcg aggtcgatgc cctcgctccg atgctcggcc
     1381 tcggatgggg gtcgaggctc gagcgcctcg acggggtcga cctttcacgc gcgaccggtc
     1441 cgacgcaggt ccacctcctc ctccacacgg gtcggctgtc gcggcggcgc gaccggcagt
     1501 cgatcgcaca ggcgctggat cgggaccgct tcgccgacgt cgtgctccgc ggccgcgacc
     1561 gggtggcgca gagcgtcctc tcgcccgagc aaccgggcta ccttcccgcc tgggcgggct
     1621 acgggggagg cggccccgtc cgggtcggac gtgagctccg gctcgtctct ccccgtgcgg
     1681 agctcctcga cctgacggcc cggtgggtcc agacggccct gtcgaccgcg ggggcggacg
     1741 tggacctcgt ggcggtggat ccacaggtgt tcgagcgcct ggtcctgcgg gagcgggact
     1801 acgagctcgc gctgtgggtc acgcgcagcg gcccggggcc gtggctcgac cgctggttct
     1861 cctcggactc gggggagcgt ccctcgacct gggaggatcc gacgctcgac gccctgctcg
     1921 gacgggtcgg cctcggggga ccggagggtg caagcgcgct cgagacctcc cagcagctcc
     1981 tcgcccagca ggcggccgtc atcccgctgt tccgcccgga ggtcgtgatg gcctcgaggg
     2041 gggtccgtgg cgtcgaggcc aaccccacgg ccgacggtcc gctgtggaac gccgaggcgt
     2101 gggcgttcgt cgaacgaccg accacacggc gaacgtgatc cgtcggcggt ctcctataat
     2161 cgaggctcct cgtcggagtg gcggaatttg gtagacgcgc taggttgagg gcctagtgcc
     2221 ccgagagggg cgtgggagtt caaatctccc ctccgacacc atcgagcagg ggcgacgccg
     2281 ggtcgtcgtt tcctcggcgc ccggcgccgt ggcaggatgg acgcatggcg ctggtcggat
     2341 gggcggtcgt ggccgcgatc gtgcttggct accagggact ggcgctcgcc agcggccccg
     2401 gcttccccac gatcagcgac ctgctgggcg tggtgacgcg cagcctcgtc ggacggtggg
     2461 tgctcttcgc cctgtggctg tggctggggt ggcacctgtt cgtccgggga tggcgcttct
     2521 tcctccaggg gtgagcgtgg acggaagcct cggcggcggg gtcctcggcg gcgtcgtggc
     2581 tccgctggcc tcggtgtacc tcgcgttcct cgcgatgctt cgcctctacc gccgctcggg
     2641 gcgcgatgac cgtcgggacc gtcaccgtgc cccggcgcgc cctctcgctc tcctgcgaca
     2701 cctcctcgtc acggccgcgg gcggcttcgg cgtcttcctc gggatcgtcc tggtcttctc
     2761 gttcgtgttc gccgatcagg agcgggccct ccgtgaggcc ctgggtgggg gaggtttcct
     2821 ggccgccctc gcggtggtgg tactcgccct cgcgggatgg atcgagggct cgatcgcctc
     2881 ccgaggttcg aggtagcccc gctggcctcc tgggcggacc tggagcgggc cgagcccggt
     2941 ttcgccgagc ggctccgcgc ccgcctctcg caccggaagc actgcaccct cgcgaccctc
     3001 cgagccgacg gatccccgcg gatcagcggg accgaggtcg tgttcgaagg cggggagacc
     3061 tcctcgggtc gatgcccggc tcgaggaaag cgcgcgacct cctccgcgat cctcgcctgg
     3121 cgctgcacgg tccgaccgaa gatcccccgg agccgccggg cccgtgggac ggcgaggcga
     3181 agctggcggg tctcgccgtc ccgatcgagc gccacgtcgc ggagggaccg ccggcggaat
     3241 ggttccgcct cgacatccgc gaggccgtcc tcacctggat cggggatcca cccgaccggc
     3301 tgctcatcga ctcgtggcac ccggagcgtg ggctacgccg ccgcgagcgc cgctagcgcg
     3361 cggcgcagcc ccgctcgaga ggccgtgcct agctcgtgac cgtgatcggg acgctgtcga
     3421 ccggcgagac cttcccgccc tggatcgcgc gcgcgtagat cgtgcgcgtc cccggctgca
     3481 cgaacgtcgt cggcatggac gccgagaacg taccggtggc gaggttcacg gtcgcgttcg
     3541 tcttcgtccc gctgaaggtc gggttgtcga tcgtcagctc gacccgtcgg ttgaagctcg
     3601 aggtgtagct cgg
//
LOCUS       DATLGG010000177         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_90312, whole genome shotgun sequence.
ACCESSION   DATLGG010000177 DATLGG010000000
VERSION     DATLGG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90312"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VM638_05045"
     CDS             <1..1078
                     /locus_tag="VM638_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11823.1"
                     /translation="EWDAWYAQRLGAPHYLLASGDARITFDELLGDPITVQTRIEDRA
                     EVTIRGHASWLGLDLRDDARLTGGSLWATGEATDRARLDITEDASWGSIDLRDDASAR
                     LVGSGGPSTGFSIRLHDRANAKIERAKAGMYQHWGSGLLEVVDTVAPAELGGQRELRI
                     WMRSADARVSLRNFGHQHSLNLPVCGMPCNPWFVIFGEGEYVRAEDSVFHMEIDRTRF
                     VELEDSVGLSVYPVRTGGGGYKDPVRGECIRSVASATNVGFEGHCARLRVSVLGSDGA
                     MLPGATVTLLDGVSGEPSPAYDAGSGEVDARRTTDAAGVATFDLARRAHPLKVRVQGP
                     GIDHTTDVFVVATDAHDLTVRLPR"
     gene            complement(1075..1356)
                     /locus_tag="VM638_05050"
     CDS             complement(1075..1356)
                     /locus_tag="VM638_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVM11824.1"
                     /translation="MIRTRVRYGRLVALLVATVTILAAGRAGAGSGAEEQPEPLQREH
                     VVRSGETLWEIASRLAAPGEDPRPLIGEIRRANDLSDSALREGQRLLLP"
     gene            1466..2080
                     /gene="lexA"
                     /locus_tag="VM638_05055"
     CDS             1466..2080
                     /gene="lexA"
                     /locus_tag="VM638_05055"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVM11825.1"
                     /translation="MGEDITPRQQRILEFIRRTVQERGYPPTVREIGEAVGLTSSSSV
                     HAQLANLQRKGYLRKDAAKPRAIEVSGGRSAAAAAVPLVGRIAAGEPVLAEEHVEEYL
                     PVPPGFVGRGEHFAVTVTGDSMIDAGIHHGDIAVIRRQDVAEDGAIVAAILPGAAEEE
                     ATVKRLRRRDGRLVLVPENPELDPFEMPKGGRIVGVVVSILRKL"
     gene            2090..>2685
                     /locus_tag="VM638_05060"
     CDS             2090..>2685
                     /locus_tag="VM638_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HVM11826.1"
                     /translation="MEPDHPRFSRRRFLSIAAVLAATRLLPRRRPLREILSTAPSASE
                     PLSLDILCRDAWGAADRRGSYVRHTVRRITLHHSAVRLEDNRDAPGRFRSHQAYHQSL
                     GWPDIAYHILIDRNGHVYRGRPAWAKGDTGTDYDPRGHLLVLCEGDFESQGPSRAQVR
                     SLVRVLAWACRRFDVPPRRIRGHRDWASTTCPGRNLYAL"
BASE COUNT          379 a          981 c          952 g          373 t
ORIGIN      
        1 ggagtgggat gcctggtacg cgcagcggct cggcgctccc cactacctgc tcgcgagcgg
       61 cgacgcgcgg atcaccttcg acgagctcct cggggacccg atcaccgtcc agacgcggat
      121 cgaggaccgg gccgaggtga cgatccgggg gcacgcctcc tggctggggc tcgacctgcg
      181 ggacgacgct cggctcacgg gcgggagcct gtgggcgacc ggagaggcga ccgaccgcgc
      241 gcggctcgac atcaccgagg acgcgtcgtg gggaagcatc gacctgcgcg acgacgcctc
      301 ggcgcgcctc gtcggcagcg gtgggccgag cacgggcttc tcgatccggc tccacgaccg
      361 ggcgaacgcg aagatcgaac gggcgaaggc ggggatgtac cagcactggg gctcgggtct
      421 gctcgaggtc gtcgacacgg tcgcccccgc ggagctcggg ggacagcgcg agctgcgcat
      481 ctggatgcgc agcgccgacg cccgggtgtc gctccggaac ttcggccacc agcattccct
      541 gaaccttccg gtctgcggga tgccgtgcaa cccgtggttc gtcatcttcg gggagggtga
      601 atacgtgcgg gcggaggatt cggtcttcca catggagatc gaccgcacgc gcttcgtcga
      661 gctcgaggac agcgtcgggc tctccgtcta tccggtccgg acggggggtg gcggctacaa
      721 ggacccggtt cggggcgagt gcatccgctc cgtcgcctcg gccacgaacg tcggcttcga
      781 gggccactgc gcgaggctcc gcgtgagcgt cctcggctcc gacggtgcga tgctccccgg
      841 cgcgaccgtg accctcctcg acggcgtgag cggcgaaccc tcccccgcct acgacgccgg
      901 cagcggcgag gtcgacgccc gtcgcacgac cgatgcagcg ggggtcgcga ccttcgacct
      961 cgcgcgacgg gctcatccgc tcaaggttcg ggttcagggg ccgggcatcg accacacgac
     1021 ggacgtcttc gtggtcgcga cggatgcgca cgacctgacg gtcaggctcc cccgctaagg
     1081 gagcaggagg cgctggccct cccgtagggc ggaatcggaa aggtcgttcg ctcgccggat
     1141 ctccccgatg agcggccggg ggtcctcgcc gggcgccgcg agccggctcg cgatctccca
     1201 cagggtctcg ccggagcgga cgacgtgctc ccgctggagg ggctccggct gctcctcggc
     1261 ccccgagccg gcacccgctc gccccgccgc caggatggtg accgtcgcga cgaggagggc
     1321 caccaagcgt ccgtatcgaa cccgtgttcg gatcacgtgc cgagtatctc gaacgcacgt
     1381 tcggatgtca aggagtcgaa cacatgtttg cccccggggc ctccccgtgc tacgttcggc
     1441 cgcgaacccg acaggaggac cgacgatggg cgaggacatc accccccggc agcagcggat
     1501 cctggagttc atccggcgaa ccgtgcagga gcgtggatat cccccgacgg tgcgcgagat
     1561 cggcgaggcc gtgggactca cctcctcctc gagcgtccat gcgcagctcg cgaacctcca
     1621 gcgcaagggg tacctgcgga aggacgccgc gaagccgcgg gcgatcgagg tctccggggg
     1681 ccgctcggcc gccgccgccg ccgtcccgct cgtcgggcgg atcgccgccg gggagccggt
     1741 cctcgccgag gagcacgtcg aggagtacct ccccgtcccg ccgggcttcg tggggcgcgg
     1801 ggagcacttc gccgtgaccg tgaccggcga ctccatgatc gatgcgggga tccaccacgg
     1861 cgacatcgcg gtgatccgcc gccaagacgt cgccgaggac ggagcgatcg tcgcggcgat
     1921 ccttccgggc gccgccgagg aggaggcgac ggtgaagcgc ctccgtcgac gcgacggacg
     1981 cctcgtcctg gtcccggaga accctgagct cgaccccttc gagatgccga agggcgggcg
     2041 catcgtcggc gtcgtggtct cgatcctgcg gaagctctag cctggcaggg tggagcccga
     2101 ccacccgcgc ttctctcgcc gtcgcttcct gtcgatcgcc gcggtgctcg cggcgacgag
     2161 gctgctccca cgccggcgtc cgctccgaga gatcctctcg accgcgcctt cggccagcga
     2221 gcccctctcg ctggacatcc tctgccgcga cgcctggggc gccgcggacc gccgcggctc
     2281 ctacgtgcgg cacaccgtcc gacggatcac cctccaccac agcgccgtga ggctcgagga
     2341 caaccgtgac gcacccggcc gcttccgctc ccaccaggcg taccaccagt cgctcgggtg
     2401 gccggacatc gcttaccaca tcctgatcga ccgcaacggc cacgtgtacc gcggccgccc
     2461 ggcctgggcg aagggcgaca ccgggacgga ctacgacccg cgtggtcacc tcctcgtgct
     2521 gtgcgagggc gacttcgagt cgcagggccc ctcgcgggcg caggtccgct cgctcgtgcg
     2581 ggtcctggcc tgggcctgcc ggcggttcga cgtccccccg cgacggatca gggggcaccg
     2641 ggactgggcc tcgaccacct gccccggacg caacctctac gcgct
//
LOCUS       DATLGG010000178         3643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_168554, whole genome shotgun sequence.
ACCESSION   DATLGG010000178 DATLGG010000000
VERSION     DATLGG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168554"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..905)
                     /gene="folK"
                     /locus_tag="VM638_05065"
     CDS             complement(414..905)
                     /gene="folK"
                     /locus_tag="VM638_05065"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HVM11827.1"
                     /translation="MTRAYLGLGSNLGDRLANLEEAVRRLDAAGARVLRSSRVYETDP
                     VGGPEQPDYLNAVVEVETELSARDLLDACLDVERQMGRVRTERWGPRRIDIDLLTYGR
                     RQIDEPGLTVPHPRMHERAFVLVPLLELDPDPPLPGGRHAVREAERETSLPAVRPFAP
                     PLR"
     gene            complement(902..1258)
                     /locus_tag="VM638_05070"
     CDS             complement(902..1258)
                     /locus_tag="VM638_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HVM11828.1"
                     /translation="MPQLLRVTGIAADGRHGASEGERDEPQRFAIDLEVVVDASDDDL
                     GSTADYRDLARVARSAVEEGSYAILETIAEQVSAAVAEVPGVLTCRAVVHKPAAADRL
                     GVADVSAEATAGRLGR"
     gene            complement(1262..1723)
                     /locus_tag="VM638_05075"
     CDS             complement(1262..1723)
                     /locus_tag="VM638_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVM11829.1"
                     /translation="MAKLDISMTPEEMETYLAEQRTARVATADERGEPHVVPLWFIWH
                     DRALFLNSTLGNVTVENMLRSGRATAVVDDGVEYEVLRGVTIHGRIERANDDPRQPEV
                     ERMWSEKYMGGGEVPYSKWRNRTWLRLDPDSIASWDFRKIPEAKARARAQG"
     gene            complement(1724..2635)
                     /locus_tag="VM638_05080"
     CDS             complement(1724..2635)
                     /locus_tag="VM638_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVM11830.1"
                     /translation="MSELDFQRVELSVGEVAYADEGDGPPVVLLHGFPTSSHLWRDLL
                     PMLSVRMRALAPDLPGYGASERRAEADSGIPALAAAVRELLDHLGIERAALVGHDIGG
                     AIAQILALEGRAEALVVIDGVTIGVEPSEPMRVLQATPDRLATPELAELTVRTAFDLG
                     MGQRARLPEEELEVFLRPWREDPPALLRAARALTETGLDGRREDLAALDIPAFVVWGE
                     DDAFLPARHAEELWETLPDAGIALLPGCGHYVTEDAAETVLPLVSQFLRNRYLGQPGH
                     DHAGGPVPVDLGVSFTRPDPPHEFVEE"
     gene            complement(2632..3474)
                     /gene="folP"
                     /locus_tag="VM638_05085"
     CDS             complement(2632..3474)
                     /gene="folP"
                     /locus_tag="VM638_05085"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVM11831.1"
                     /translation="MNDYRWRLPDRVLDCGERTLVMGIINVTPDSFSDGGRFFDPEVA
                     VAEGVRMVADGADILDVGGESTRPGSDAVPAADERDRVVPVVKRLVAEVDVPISIDTR
                     KAEVAAAALEAGATIVNDVTAGRDPEMFGLVRDSGAALVLMHMKGEPKTMQENPVYDD
                     VVREVHDYLAERVEAAVEAGIERERVAIDPGLGFAKTTPHSLTLMKETPAFLDLGRPL
                     LVGPSRKSFIGDALRGLPVGERMEGTAAAVAAMAFLGAHIVRVHDVPQMVRVVRVADA
                     IRRA"
BASE COUNT          533 a         1353 c         1259 g          498 t
ORIGIN      
        1 caccgccgtt gcggatgcgc tccccgaccc gcaccagttc ccgccccttc tcatccagca
       61 cggccccgcc accgggctct gggcggaacg aggtcgacac cggccatacc gggaaggcca
      121 cgtcgccgcc tccgaccagc agcaggcaat caccgcggat caccaagcgg ccctcgaggc
      181 ccgcctggcc gaacgcgcgc gtatccgcta ccggaaagag taagccttgg gcttccggcg
      241 gcagtgccgg cgagggagaa gcacgtgagg tccgcggtcg agcccggtcg acatctcggg
      301 gtcccgcaca cccaaccacg acgagggtga gacacagcaa cgctcggagg gaatggaacc
      361 tggtcacgcc accatcgcag ccatcgtagc cgagctgcgc tatcgggcga tccctagcgg
      421 agaggcggag cgaacgggcg aaccgccgga agggacgtct cccgctccgc ttcgcggacc
      481 gcgtggcgtc cgccggggag cggcggatcg gggtcgagct ccaggagggg gacgaggacg
      541 aacgcgcgct cgtgcatcct cgggtgcggg acggtgaggc cgggctcgtc gatctgccgc
      601 cggccgtagg tcaagaggtc gatgtcgatc ctgcgtggcc cccaacgttc ggtgcggacg
      661 cgtcccatct gccgctcgac gtcgaggcag gcatcgagga gatcgcgcgc ggagagctcc
      721 gtctcgacct cgacgaccgc gttcaggtag tccggctgct cgggtccgcc gacgggatcg
      781 gtctcgtaaa cgcgcgagga gcggaggacg cgcgcgccgg cggcgtccag tcgccggacc
      841 gcttcctcca ggttcgcaag acggtcgccg aggttcgagc cgagcccgag gtaggcgcgg
      901 gtcaccgccc gagccggccc gcggtcgcct ccgccgacac gtcggccacc cccagccggt
      961 cggcggccgc gggcttgtgg acgacggcgc gacaggtcag gacacccgga acctccgcga
     1021 cggccgcgct cacctgctcg gcgatcgtct cgaggatcgc gtacgacccc tcctccacgg
     1081 ctgagcgggc gacgcgcgcc aggtcgcggt agtcggcggt cgacccgagg tcgtcatccg
     1141 aagcgtccac cacgacctcg aggtcgatcg cgaaccgctg aggctcgtca cgctcaccct
     1201 cgctcgcgcc gtgccgaccg tcggcggcga tcccggtcac ccggaggagc tgcggcatgc
     1261 ctcagccctg cgcgcgggcg cgcgccttcg cctcggggat ctttcggaag tcccaactcg
     1321 cgatcgaatc ggggtcgagg cggagccagg tgcggttccg ccacttcgag tacggcacct
     1381 caccgccccc catgtacttc tccgaccaca tccgctcgac ctcgggctgg cgggggtcgt
     1441 cgttggcccg ctcgatgcgc ccgtggatcg tgacaccgcg caggacctcg tactcgacgc
     1501 cgtcgtcgac gacggccgtc gcgcggccag atcgcagcat gttctcgacg gtgacgttgc
     1561 cgagcgtcga gttgaggaag agcgcccggt cgtgccagat gaaccacagc gggacgacgt
     1621 gtggctcgcc tcgttcgtcg gccgtcgcca cccgcgccgt ccgttgctcc gcgaggtacg
     1681 tctccatctc ctccggcgtc atcgagatgt cgagcttcgc catctactcc tccacgaact
     1741 cgtgcggcgg gtcgggccgc gtgaaggata cgccgaggtc caccggcacg ggccccccgg
     1801 cgtgatcgtg ccccggctgg ccgaggtagc ggttgcgcag gaactgcgag acgagcggaa
     1861 gcacggtctc ggccgcgtcc tcggtgacgt agtggccgca gccgggcagc agcgcgatgc
     1921 cggcatccgg cagcgtctcc cagagctcct ccgcgtgccg cgccggcagg aaggcgtcgt
     1981 cctcccccca gaccacgaac gccgggatgt cgagcgcggc gaggtcctca cgccgaccgt
     2041 cgagccccgt ctcggtcaag gcacgcgcgg cgcgcaggag cgccggcggg tcctcgcgcc
     2101 agggccgcag gaacacctcg agctcctcct ccggcagcct cgcgcgctgc cccatgccga
     2161 ggtcgaacgc cgtgcgcacc gtgagctccg cgagctccgg cgtcgcgagg cggtcgggcg
     2221 tcgcctgcag cacgcgcatc ggctcggacg gctcgacgcc gatcgtcacg ccgtcgatca
     2281 cgaccagtgc ttcggctcgc ccttcgaggg cgaggatctg ggcgatcgcg ccaccgatgt
     2341 cgtgcccgac cagcgccgcg cgctcgatgc cgaggtggtc gagcagttcg cggaccgcgg
     2401 cggcgagcgc ggggatcccg gagtccgcct ccgcgcgcct ctcggacgcg ccgtaaccgg
     2461 gcaggtcggg cgcgagggcg cgcatccgca ccgagagcat cggcaggagg tcccgccaga
     2521 ggtgcgacga cgtcgggaag ccgtggagca ggacgaccgg tggtccatcg ccctcgtcgg
     2581 cgtacgcgac ctctccgacg gagagctcga cgcgttggaa gtcgagctcg ctcatgcgcg
     2641 ccggatcgcg tccgcgaccc ggaccacgcg gaccatctgg ggaacgtcgt ggacccggac
     2701 gatgtgtgcg ccgaggaacg ccatcgccgc gacggccgca gccgtcccct ccatccgctc
     2761 cccgacgggg agcccacgga gcgcgtcgcc gatgaacgac ttccgcgacg gcccgacgag
     2821 cagcggccgc ccgaggtcga ggaacgccgg tgtctccttc atgagcgtga gcgagtgcgg
     2881 tgtcgtcttc gcgaacccga gccccgggtc gatcgcgacc cgctcccgct cgatccccgc
     2941 ctcgaccgcg gcctcgaccc gctccgcgag gtagtcatgg acctcgcgca cgacgtcgtc
     3001 gtacacgggg ttctcctgca tcgtcttcgg ctcgcccttc atgtgcatca gcacgagtgc
     3061 ggcgccgctg tcgcggacga ggccgaacat ctcggggtcg cgcccggcgg tcacgtcgtt
     3121 gacgatcgtc gcgcccgcct ccagcgccgc ggcggcgacc tcggccttcc gcgtgtcgat
     3181 cgagatggga acgtcgacct cggcgacgag ccgcttcacg accgggacca cgcgatcgcg
     3241 ctcgtcggcc gcggggaccg cgtccgagcc ggggcgtgtg gactcgccgc cgacgtcgag
     3301 gatgtccgca ccgtcggcca ccatccggac gccctcggcc accgcgacct ccgggtcgaa
     3361 gaaccgcccc ccgtccgaga acgagtccgg cgtgacgttg atgatcccca tgaccagcgt
     3421 ccgctcgcca cagtcgagca cgcgatcagg cagcctccac cggtagtcgt tcacgagcgg
     3481 aagcctaccg gacccgtaac gatcgccgcc cctcgggcgt ttccttcacg atgacgctca
     3541 cgatggcagc aaccctggcc gagcaggcgg cggtcccccc caccttcgag tgcttctacg
     3601 aggcgacgta cgagcgcctc ctgagggcga tgtacctagt ggc
//
LOCUS       DATLGG010000179         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_227555, whole genome shotgun sequence.
ACCESSION   DATLGG010000179 DATLGG010000000
VERSION     DATLGG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227555"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VM638_05090"
     CDS             complement(<1..1819)
                     /locus_tag="VM638_05090"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HVM11832.1"
                     /translation="MTGDRVGNGLTFKRFFTDEGVHPFDQVEWETRDAIIPNFKEGGN
                     AFEQRDVEFPRSWSQNATNIVAQKYFRGPLGTPQREYSVRQMIGRVVDTITGWGRKDG
                     YFATEHDAEIFAAELTHLLVHQKAAFNSPVWFNVGVEEKPQCSACFILAVDDTMTGIL
                     NWYVEEGTIFKGGSGSGINLSTIRSSKERLSSGGLASGPVSFMRGADASAGTIKSGGK
                     TRRAAKMVVLDVDHPDVEDFIWCKANEERKARALRDAGFDMDLDGKDSASVQYQNANN
                     SVRVTDEFMQAYVDDRDWKLKAVTNGEPLESVRARDVMRQIAQAAWECADPGVQYDTT
                     INDWHTSPASGRINASNPCSEYMHLDNSACNLASLNLLKFADADGNFDIAAFKHAVEI
                     VFTAQEIIVGNSSYPTEKIGRNAKAFRQLGIGYANLGALLMARGLPYDSDGGRAWAGA
                     ITAILTGHAYTTSAKIAEQMGPFAGFAPNRDAMLRVMRKHRSAADRIDAELVPEALLS
                     AAKSSWDRAISLGETHGYRNAQASVLAPTGTIGLMMDCDTTGIEPDLALVKSKKLVGG
                     GTMQIVNQTVPRALTRLGYTDQQVTDIVDYIAEHNSVGDA"
     gene            complement(2003..>2504)
                     /gene="nrdR"
                     /locus_tag="VM638_05095"
     CDS             complement(2003..>2504)
                     /gene="nrdR"
                     /locus_tag="VM638_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HVM11833.1"
                     /translation="SKMLCVVRCQRCGKPDTRVVDSRPADAGATVRRRRECPSCGARF
                     TTFERAEEVHLVVIKRDGTKEPYDRAKVVGGIAKAIKNRPVTEEQVERLADRVQERLR
                     RKGPQVTSQDVGLEVLGALRRTDEVAYMRFASVYKGFQELTDFERELGLLLQKRQPKG
                     RATRKR"
BASE COUNT          344 a          870 c          843 g          447 t
ORIGIN      
        1 gtgcgtcgcc gacggagttg tgctcggcga tgtagtcgac gatgtcggtg acctgctggt
       61 cggtgtagcc gagccgggtg agcgcccgcg ggacggtctg gttgacgatc tgcatcgtcc
      121 cgccgccgac gagcttcttc gacttcacga gcgcgaggtc gggctcgatg ccggtcgtgt
      181 cgcagtccat catgagcccg atcgtgcccg tgggagccag cacgctggcc tgcgcgttgc
      241 ggtagccgtg cgtctcgccg aggctgatcg cccggtccca ggagctcttc gccgcggaga
      301 gcagcgcctc gggcacgagc tccgcgtcga tccggtccgc cgccgagcgg tgcttgcgca
      361 tcacgcggag catcgcgtcg cggttcggcg cgaagccggc gaagggcccc atctgctcgg
      421 cgatcttcgc cgaggtcgtg taggcgtggc ccgtgaggat cgcggtgatc gcgcccgccc
      481 acgcgcgccc gccgtcggag tcgtacggga ggccccgggc catgagcagc gccccgaggt
      541 tcgcgtaccc gatgccgagc tgtcggaagg ccttggcgtt ccgcccgatc ttctcggtcg
      601 ggtagctcga gttgccgacg atgatctcct gcgcggtgaa cacgatctcg accgcgtgct
      661 tgaacgccgc gatgtcgaag ttcccatcgg cgtcggcgaa cttcagcagg ttgaggctcg
      721 cgaggttgca cgccgagttg tcgaggtgca tgtactccga gcacgggttc gaggcgttga
      781 tccgtcccga ggccgggctc gtgtgccagt cgttgatcgt cgtgtcgtac tggacgcccg
      841 ggtcggcgca ctcccaggcg gcctgcgcga tctgccgcat gacgtcacgg gcacggacgc
      901 tctcgagcgg ctcgccgttc gtcaccgcct tgagcttcca gtcgcggtcg tcgacgtagg
      961 cctgcatgaa ctcgtcggtc acccgcacgg agttgttcgc gttctggtac tggaccgagg
     1021 ccgagtcctt gccgtcgagg tccatgtcga acccggcgtc acgcagggcc cgcgccttgc
     1081 gctcctcgtt cgccttgcac cagatgaagt cctcgacgtc ggggtggtcc acgtcgagca
     1141 cgaccatctt cgcggcgcgc cgggtcttgc ccccggactt gatcgtgccc gcggaggcgt
     1201 cggcgccgcg catgaacgag accggcccgg aggcgaggcc gccgctcgag aggcgctcct
     1261 tcgacgagcg gatcgtcgag aggttgatgc cggaaccgga gccgcccttg aagatcgtcc
     1321 cctcctcgac gtaccagttg aggatgcccg tcatcgtgtc gtcgacggcg aggatgaagc
     1381 acgcggagca ctgcggcttc tcctcgaccc cgacgttgaa ccacaccggc gagttgaacg
     1441 cggccttctg gtggacgagg aggtgcgtga gctcggcggc gaagatctcg gcgtcgtgct
     1501 ccgtcgcgaa gtagccgtcc ttccgccccc agccggtgat cgtgtcgacc acgcggccga
     1561 tcatctgccg gacggagtac tcgcgctgag gggtgccgag ggggccgcgg aagtacttct
     1621 gcgcgacgat gttcgtcgcg ttctgcgacc aggagcgggg gaactcgacg tcccgctgct
     1681 cgaaggcgtt cccgccctcc ttgaagttcg ggatgatcgc gtcgcgggtc tcccactcga
     1741 cctggtcgaa ggggtggacc ccctcgtcgg tgaagaaccg cttgaacgtc aggccgttgc
     1801 cgacccgatc ccccgtgatc cgctgaacgt catgctcccg cgtcgccacg ccagtccccc
     1861 tttctccgcc ggccgggaag gtggccctcg gaatgacagc ggtgtgattc tacgccggtg
     1921 cccccgggcg cgcaaccatc ctacgacgca gatgttgtgc ccgtccaggt tcctaccaca
     1981 atatgttgtg tctctccgga catcaccgct tccgtgtcgc acgacccttc ggctgccgct
     2041 tctggaggag cagaccgagc tcgcgctcga aatccgtgag ctcctggaag cccttgtaga
     2101 ccgaggcgaa ccgcatgtac gcgacctcgt cggtccggcg cagggccccg aggacctcga
     2161 gaccgacgtc ctggctcgtc acctgcggac ccttgcgccg gagacgctcc tgcacgcggt
     2221 cggccagccg ctcgacctgc tcctccgtca ccggccggtt cttgatcgcc ttcgcgatgc
     2281 ccccgacgac cttcgcccgg tcgtagggct ccttcgtccc gtcgcgcttg atcacgacca
     2341 ggtggacctc ttcggcgcgc tcgaaggtcg tgaagcgcgc gccgcaggag gggcactccc
     2401 gccggcggcg gaccgtcgcc ccggcgtcgg ccgggcgcga atcgaccacc cgcgtatccg
     2461 gcttcccgca ccgctggcag cgcacgacac acaacatctt ggac
//
LOCUS       DATLGG010000180         6826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_90401, whole genome shotgun sequence.
ACCESSION   DATLGG010000180 DATLGG010000000
VERSION     DATLGG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90401"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      34..170
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            271..513
                     /locus_tag="VM638_05100"
     CDS             271..513
                     /locus_tag="VM638_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11834.1"
                     /translation="MTRAMGDAYAVLEALREMPALEPSLESSTGDLLARPDGTLVPIG
                     EPGSSGEEAELPGGEPEAPLRSQRPASSRAASTLWD"
     gene            561..1409
                     /locus_tag="VM638_05105"
     CDS             561..1409
                     /locus_tag="VM638_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HVM11835.1"
                     /translation="MTTPEHYSRIDAVIDLPLMRSKTVAVCGAGAVGSRIADELARSG
                     IGALILVDPEPFEASNLSRHVLGYRDVGRNKAEALADHLRRQVPDLQVATSPADLADL
                     PDDALDSVLWWADVIVAATDDRVAQRRIGSSARRRSIPAVFPGVAPEGGGGEIFVSFG
                     WETPCFGCWDRFRSATERPRAVQALGVDILPVVHVAVSAVLALLDPASRFAHIFRPQS
                     PDPRLRTLFFAGTFRMDEGRVADGYRRAVPEFRAACPDCDPWGWRLPRGGPGHRHAFP
                     PGDDEE"
     gene            1440..1862
                     /locus_tag="VM638_05110"
     CDS             1440..1862
                     /locus_tag="VM638_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11836.1"
                     /translation="MVDTLNHELIGIRKPFGMFGQINWSVESVRGETIQVSIWTPRPL
                     DGLGVATNVRNALLNYLQDLLPLRRCRVLFERRLDGVREEHLLDFEYRPGEERKSAAI
                     LIEANVAPRRERLRPLLRRLYRDDGHAEAPDRLPKGGA"
     gene            2147..2638
                     /locus_tag="VM638_05115"
     CDS             2147..2638
                     /locus_tag="VM638_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11837.1"
                     /translation="MSAGVPHLWRPGRPETAQCHWVAGRVRVPGVNHAPFERCRCGIY
                     AFRELDHLIEQGPNPWVEPVVLGAVRLWGKVIVHDLGYRAQHAKVTEIFAVPGQEWLH
                     DVARRYRAEVTDHLVERLAWYRGGPRDGEIRWPDAATEAGAHFWTGTTYRYGRPSGRK
                     RPA"
     gene            2753..3055
                     /locus_tag="VM638_05120"
     CDS             2753..3055
                     /locus_tag="VM638_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11838.1"
                     /translation="MAVDERRRKALHDALERRIGSEEAATLMEMLPPVGWADVATKHD
                     LHQLELRLEARIEGGLAGVRGELGSAIERMGRRIVMWTAVMLVGLAGVVLTAARLS"
     gene            complement(3107..3742)
                     /locus_tag="VM638_05125"
     CDS             complement(3107..3742)
                     /locus_tag="VM638_05125"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVM11839.1"
                     /translation="MVPRVEATVVGDDGIRRCWWCAGDALYERYHDTEWGRPVADDVR
                     LFEKLSLEGFQSGLSWLIILRKREGFRRAFRGFEPAAVARFNSRSVERLMGDGSIVRN
                     RAKIEATINNARRYLELVGEFGSLARYVWGFEPDAKDRPGRVVLEWLFANPKTPASIA
                     MSKDLRKRGWNFVGPTTVYAFMQAMGLVNDHVEDCDLRTEVERDRTRFRRP"
     gene            3817..4005
                     /locus_tag="VM638_05130"
     CDS             3817..4005
                     /locus_tag="VM638_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11840.1"
                     /translation="MIVFVVVLAFVAAIVTVLRLTRPQQADAPPADPNDVLARFLAKW
                     IIAGFILWLVLMLFGEPW"
     gene            4096..4617
                     /locus_tag="VM638_05135"
     CDS             4096..4617
                     /locus_tag="VM638_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11841.1"
                     /translation="MKTKEIAERLASLCAEAKMQTSAGRILVPARPVDLIEELLPFLD
                     DREREGALWNGFAHMARRSRSIGWRSLLQFCWDERESSIDLLTVEGLSFVALPVDEDG
                     TAERVIARFDPEVSGDLVAAFLLDVARSNGEEYGFELFDVLPDEAIDRVGLPYGAIED
                     AYRAWTHRQETSR"
     gene            4643..5605
                     /locus_tag="VM638_05140"
     CDS             4643..5605
                     /locus_tag="VM638_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HVM11842.1"
                     /translation="MDQLITLLGGGLPPREELADRYRGTMLGVAVGNALGLHVEGASA
                     ATVAERFGHITDIDPKVRETPWDDDLAQTVVLAEALLEGDDLDLDDLARRLLAWREAN
                     GRGIGALTWQVLDELEEGTPAHDAARVVWERTGRSSAGNGAVMRCWPVALRWLHDPER
                     LVREARTSALVTHHDPRCEWSTVASVMLAAGTLLGRDLELPRLARALEEAGAPAEVTD
                     ALRAAEAASLDDLALDDPFAMGYTLKAMQVAAWAGRQEPDVARVLSDVVSAGGDTDTN
                     GAVAGAIMGARLGARAIPQRWIDAIPRPEDLVTVADDLLTRVQA"
     gene            complement(5773..6159)
                     /locus_tag="VM638_05145"
     CDS             complement(5773..6159)
                     /locus_tag="VM638_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVM11843.1"
                     /translation="MSGGTFDFPVCPTLPAADLARAKEWYREKLGVTPAEELPEGLIY
                     RTGGGRWDLYPSASADTNQATAATIIVDDVDRVVDELTARGVTFERYDVPGLQTDARG
                     VGVFETAKMAWFKDSEGNILAVEQRI"
     gene            complement(6156..>6826)
                     /locus_tag="VM638_05150"
     CDS             complement(6156..>6826)
                     /locus_tag="VM638_05150"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM11844.1"
                     /translation="RVLDVACGTGVVAREVADRLGAAAVVGVDLNQAMLAVARRLRPD
                     IAWRQADAAALPFADDSFDAVLCQAALMFFPDRAGALREMVRVAKPHGKVVAQVWDDL
                     GRQPAYGPFVEVVADRLGAEAKRVLDSYWVLGDIDLVRALFEAAGLEVTEARTHLETA
                     RFPSVEEMLRAEIEATPLIDRISDEDHARVLADAGDALRGFEREDGTADIPVGGHVVV
                     GRKR"
BASE COUNT         1002 a         2427 c         2398 g          999 t
ORIGIN      
        1 gagcgtcggt gtcagggcgg tggtgtacac tacgttctgc atcaggagtg gctcggcggt
       61 agtcgggccc agcaacctgg gacggacacc caaggtgctt cccgctgagg cggggatgcg
      121 gtcacgaagg gccggacaag cggcccggag gggcaaacaa gtgtcctgaa ggcaggagct
      181 cggcctggtg cggaggccga gctttttgcg tttcgggccg ccgcatcccg gctcggcctg
      241 gcaccgaccg tcccccgagt gggaagggag gtgacacgcg cgatgggaga cgcgtacgcg
      301 gtcctcgagg ccctgcggga gatgcctgcc ctggagcctt ctctcgagag ctcgaccggc
      361 gacctgctcg ctcgccccga cggcacactg gtgccgatcg gcgagccagg gtcctcgggc
      421 gaggaggcgg agctgccggg cggcgagccg gaagcgccgc tccgcagcca gcggccggca
      481 tccagccggg ctgcctcgac gctctgggac tgaaggttgg gggcgggttc gcccgccccc
      541 tccttcccat cacgcacgcc atgacgaccc ccgagcacta cagccggatc gacgcggtga
      601 tcgacctgcc gctgatgcga tcgaagaccg ttgcggtctg cggcgcgggc gccgtcggct
      661 cgcgcatcgc cgacgagctc gcgcgctccg ggatcggcgc gctgatcctc gtggaccccg
      721 agccgttcga ggcgagcaac ctctcgcggc acgtcctcgg ataccgcgac gtcgggcgga
      781 acaaggccga agcgctggcc gaccacctca ggcgccaggt gccggacctg caggtcgcga
      841 cgtccccggc cgacctggcc gacctacccg acgacgcgct ggacagcgtc ctctggtggg
      901 ccgacgtcat cgtggccgcg accgatgacc gcgtcgcgca acgacgcatc ggatcttcag
      961 cccgccgccg ctcgatcccc gcggtcttcc ccggcgtcgc tcccgaggga ggcgggggtg
     1021 agatcttcgt ctccttcggc tgggagactc cgtgcttcgg gtgctgggac cggttccgca
     1081 gcgccactga gcgtccgcgg gccgtgcagg cgctgggcgt ggacatcctg ccggtggtgc
     1141 acgtcgctgt ctcggcggtg ctggcgctgc tggacccggc gtcgcgcttc gcccacatct
     1201 tccggccgca atctcccgat ccgaggctcc ggaccctctt cttcgcgggc accttccgca
     1261 tggatgaggg gcgggtggcc gacggttacc gacgggccgt cccggagttc cgggccgctt
     1321 gcccggactg cgatccctgg ggctggcgac tgccccgagg cgggccgggg caccggcatg
     1381 cgttcccgcc aggcgatgac gaggagtagg agggagacga gatcgatcag aaacgggtct
     1441 tggtcgacac actgaaccac gagctgatcg gcatcaggaa gccgttcggg atgttcgggc
     1501 agatcaactg gtcggtcgag tccgtgcggg gggagacgat ccaggtctcg atctggacgc
     1561 cgagaccgct ggacgggctg ggggtggcga cgaacgtgcg gaacgcgctc ctcaactacc
     1621 tccaggacct cttgcccctg cgtcggtgca gggtcctgtt cgagcggcga ctcgacggcg
     1681 tgcgcgagga gcacctgctg gacttcgagt accggccggg cgaggagcgg aagagcgccg
     1741 cgatcctgat cgaggcgaac gtcgcgcccc gccgcgaacg gctgaggccc ctgctccggc
     1801 gcctgtaccg ggacgacggc cacgccgaag cccccgaccg gctgccgaag gggggcgcct
     1861 agaggtccat caccggaact tgtcagaccc cagtggcact atccgcaggc aggtcccttg
     1921 acagcagtgc gaccatggag gcgaacagtg aacataggac gggaacgcga gaccatctac
     1981 atcgagccgg tcgaggcgcc ggcccccacc cccgtgccac ccgacgaccc gccggagaag
     2041 gaacccgccg aggtcggctg agccggtgcg cgtccccgac tcccccgtgc ctctccgtgc
     2101 gttccgcgcc tggaggatcc gccgagacca gagcggcatc acgctggtgt ccgcgggtgt
     2161 gccccatctg tggcgacccg gccggccgga gacagcccag tgccactggg tcgccggtcg
     2221 cgtccgcgtc ccgggggtga accacgcgcc gttcgagcgc tgcaggtgtg ggatctacgc
     2281 gttccgtgag ctcgaccacc tgatcgagca ggggccgaac ccgtgggtcg agccggtggt
     2341 gctcggtgcc gtccggctgt gggggaaggt gatcgtgcac gacctcggct accgggcgca
     2401 gcacgcgaaa gtaaccgaga tcttcgcggt ccccgggcag gagtggctgc acgacgtcgc
     2461 ccgccgctac cgcgccgagg tgaccgacca tctcgtggag cggctcgcct ggtatcgggg
     2521 cggtccgcgc gacggcgaga tccgctggcc cgacgccgcg accgaggccg gcgcgcactt
     2581 ctggacgggg accacgtacc gctacggccg accgagcggc cggaagagac ccgcctgacc
     2641 ctcgcgccgc caggatccga tgtgccggcg acgtcagagc ctcctgccac cgtcggatcg
     2701 cccatcctct cccgggccag tgggttgttc gctccacgga tgaggggaga caatggcggt
     2761 tgatgaacgc agacggaagg cgcttcacga tgcgctcgag cggcgcatcg gctccgagga
     2821 ggcggcgacg ctgatggaga tgctgccgcc ggtcggctgg gcagacgtcg cgacgaagca
     2881 cgacctgcac cagttggagc ttcggctgga agcgcggatc gagggaggcc tcgcgggcgt
     2941 tcgaggtgag ctcgggtccg cgatcgagcg catgggccgg cggatcgtca tgtggaccgc
     3001 cgtgatgctc gtcggcctcg ccggggtcgt cctcaccgcg gcgcgcctct cctagcaggg
     3061 ttcgcggacc ggaaccctcg gcccagagcc cgccggttcc gtatcgctag ggccggcgga
     3121 agcgggtgcg gtcgcgctcc acctccgtgc gcaggtcgca gtcctcgacg tggtcgttga
     3181 cgaggcccat cgcctgcata aaggcgtaca cggtcgtggg gccgacgaag ttccagccgc
     3241 gcttgcgcag gtccttgctc atcgcgatcg aggcgggcgt cttcgggttg gcgaacagcc
     3301 actcgaggac gacccgtccg ggccggtcct tcgcgtccgg ctcgaacccc cagacgtagc
     3361 gcgcgaggct gccgaactcg ccgacgagct cgaggtagcg gcgggcgttg ttgatcgtcg
     3421 cctcgatctt cgcgcggttg cggacgatgc tcccgtcgcc catcagtcgc tcgacgctgc
     3481 ggctgttgaa ccgcgccacc gccgccggtt cgaacccgcg gaacgcgcgg cggaacccct
     3541 cgcgcttgcg caggatgatc agccagctca gcccggactg gaacccctcg aggctcagct
     3601 tctcgaacag ccggacgtcg tcggcgaccg gacggcccca ctcggtgtcg tggtaccgct
     3661 cgtacagcgc gtcgccggcg caccaccagc aacgccggat cccatcgtcg ccgacgaccg
     3721 tcgcctccac gcgcggaacc atctcacgtc gccgcccgcc gcggccaccg ccgcacccgt
     3781 ccggttcgtg tcggcggcca ccggcatcat cggcgcgtga tcgtcttcgt cgtcgtgctc
     3841 gcgttcgtcg ctgccatcgt cacggtcctt cggttgaccc ggccgcagca ggcagacgcc
     3901 ccgccggcgg acccgaacga cgtgctcgcc cgcttcctgg cgaagtggat catcgccggg
     3961 ttcatcctct ggctcgtgct catgctcttc ggagagccct ggtgaagtcg cggcacccga
     4021 tcgtcccgct cccccatcga acgcgcgttc gtgtcggtgc ctcccggcat catccgaggt
     4081 ggagagcgtg gccccatgaa gacgaaggag atcgccgagc gcctcgcgtc gctgtgcgcc
     4141 gaggcgaaga tgcagacgtc cgccggacgg atcctcgtgc cggcgcggcc cgtcgacctc
     4201 atcgaggagc tcctgccgtt cctcgacgat cgcgagcgcg agggcgccct gtggaacggc
     4261 ttcgcccaca tggcccggcg ctcccggtcg atcggctggc ggtcgctcct gcagttctgc
     4321 tgggacgagc gggagagctc gatcgacctc ctgacggtcg agggtctgtc gttcgtcgcg
     4381 ctgccggtcg acgaggacgg cacggccgag cgcgtgatcg cccggttcga cccggaggta
     4441 agcggcgacc tcgtcgccgc cttcctgctc gacgtcgcgc ggtcgaacgg ggaggagtac
     4501 ggcttcgagc tcttcgacgt gttgccggac gaggcgatcg accgggtcgg gctgccgtac
     4561 ggtgcgatcg aagacgcgta ccgcgcgtgg acgcaccgac aggaaacgag ccgatgagcc
     4621 ggcgcacatg acggagggac gcatggatca gctgatcacc ctgctcggcg gagggctccc
     4681 gccgcgcgag gagctcgcag atcgctaccg cggcacgatg ctcggcgtgg cggtcggcaa
     4741 cgcgctcggc ctgcacgtcg agggtgcctc cgcggcgacc gtcgccgagc gcttcggcca
     4801 catcaccgac atcgacccga aggtgcggga gacgccgtgg gacgacgacc tcgcgcagac
     4861 ggtcgtgctg gcggaggcgc tgctcgaggg ggacgacctc gacctcgacg acctcgctcg
     4921 ccggctgctc gcatggcggg aggcgaacgg acgcgggatc ggggcgctca cgtggcaggt
     4981 gctcgacgag ctcgaagaag gaacgcccgc ccacgatgca gcgcgcgtcg tctgggaacg
     5041 caccggacga agctccgccg ggaacggcgc ggtgatgcgg tgctggcccg tcgccctccg
     5101 gtggctccac gatcccgagc ggctcgtgcg cgaggcgagg acgagcgccc tcgtgacgca
     5161 ccacgacccg cggtgcgagt ggtcgaccgt ggcctcggtg atgctcgccg ccgggacgct
     5221 cctcggtcgc gacctcgagc tccccaggct cgcacgtgcg ctcgaggagg ccggagcccc
     5281 tgcagaggtc acggacgcgc tgcgtgccgc cgaggccgcc tccctcgacg acctcgcgct
     5341 cgacgacccg ttcgcgatgg gctacacgtt gaaggcgatg caggtcgccg cgtgggccgg
     5401 tcgccaggag ccggacgtgg cacgcgtcct cagcgacgtc gtcagcgccg gaggcgacac
     5461 cgacacgaac ggagcggtgg cgggcgcgat catgggcgcg cgtctcggcg cccgagcgat
     5521 cccccagcga tggatcgacg cgatcccgcg ccccgaggac ctggtgaccg tcgccgacga
     5581 cctcctgact cgggtccagg cgtagcagag cgacgtccgg ggaaggctcc aagcagagga
     5641 ggtgagctga tgtacatacc gatcggcatc ggagcgatcg tcgtcatcgc gctcctgatc
     5701 tggctcctcg cctgagctag cagcccacaa cgacggggcg ggcccgcgcc gccccgtcac
     5761 catcccccga gctcagatcc tctgctcgac cgcgaggatg ttcccttcgc tgtccttgaa
     5821 ccaggccatc ttcgcggtct cgaagacgcc gacccctctt gcgtccgtct ggaggcccgg
     5881 aacgtcgtac cgctcgaagg tcacgccgcg ggcggtcagc tcgtcgacga cgcggtccac
     5941 gtcgtccacg atgatcgttg ccgccgtcgc ctggttcgtg tcggcggacg cgctcggata
     6001 gaggtcccac cgccctcccc ccgtacggta gatcaatccc tccggaagct cttcggccgg
     6061 cgtcaccccg agcttctcgc ggtaccactc cttcgcccgt gcgaggtccg ccgccggcaa
     6121 agtgggacag accgggaagt cgaacgtccc gccgctcatc gcttcctccc gacgacgaca
     6181 tgacctccga ccgggatgtc ggccgtgccg tcctcccgtt cgaatccgcg cagcgcgtca
     6241 ccggcgtccg ccaagacgcg ggcgtgatcc tcgtctgaga tgcggtcgat cagcggggtc
     6301 gcctcgatct cggcacgcag catctcctcc acggagggga agcgagcggt ctccagatgc
     6361 gtccgagcct ccgtgacctc gagcccagcc gcctcgaaca gcgcccgcac cagatcgatg
     6421 tcgccgagga cccagtagct gtcgagcaca cgcttcgcct cggcaccgag acgatccgcg
     6481 accacttcca cgaacgggcc gtacgcgggt tgtcttccga ggtcgtccca tacctgcgcg
     6541 acgaccttgc cgtgcggctt ggcgacacga accatctccc gcaacgctcc ggcgcggtcg
     6601 gggaagaaca tgagcgccgc ctggcacagg accgcgtcga acgagtcgtc ggcgaagggg
     6661 agggcggctg cgtcagcctg gcgccatgcg atgtccggcc ggaggcgccg tgcgaccgcc
     6721 agcatcgctt ggttcagatc cacgccgacg accgccgcgg ctccgagccg gtcggccacc
     6781 tcccgcgcga ccacgccggt cccgcacgcc acgtcgagca cacgat
//
LOCUS       DATLGG010000181         3490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_211925, whole genome shotgun sequence.
ACCESSION   DATLGG010000181 DATLGG010000000
VERSION     DATLGG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211925"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VM638_05155"
     CDS             complement(<1..435)
                     /locus_tag="VM638_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVM11845.1"
                     /translation="MAELIAQNLAPIMFATLVVFMLTGYPVAFALAANGLLFFLIAVE
                     LAPYAPQTITLSWPLLQALPERVYGTMANEVLLAIPFFTFMGLILERSGMAEDLLDTI
                     GQLFGTVRGGLAYAVIFVGALLAATTGVVAASVISMGLISLPI"
     gene            complement(461..1093)
                     /locus_tag="VM638_05160"
     CDS             complement(461..1093)
                     /locus_tag="VM638_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HVM11846.1"
                     /translation="MLLAFSRGIDALTVRLGKITAWAIVVAIVVSAANAIIRKVFGTS
                     SNAWLELQWYLFGAVFMLCAAWTLKDNEHIRIDIVVSRFSKRTRDWIDVLGHVLFLLP
                     FVAVMIWLSWPFFWRSYHSGEISTNAGGLIIWPAKALILLGFMVLLLQWASELIKRVA
                     VMRGEIEDPHHGAGGHGAAAESEAERLLAELAPPQPSTEVSGAPPVDPKR"
     gene            1304..>3490
                     /locus_tag="VM638_05165"
     CDS             1304..>3490
                     /locus_tag="VM638_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HVM11847.1"
                     /translation="MHSLAVIAVSGAYLGLLFGIAWWADRKAADGSFARTFGPWAAIT
                     FALTLAIYNTSWSFYGSVGRAAASGLDFLPIYIGPTLVLLFGHRMLAKVIAIAKAQNV
                     TSISDFIAARYGKSQLIAALVTVTALIGVLPYIALQLKAVGTSFDVLTSPGPAGTDEI
                     VPFWRDSAFAVALAMAAFTIVFGVRHINASEHHRGLMVAIAFESLVKLGAFLAVAAFI
                     VFGMSDGIGALLDRAAESPGLRPLLTVDLGQPAWFATTLIAVIAFLCLPHAFHVAVVE
                     NDDPRHARFAAWFYPVYLAALSVFMVPIAIVGLTTLDASINPDTFVISLPIAADRPGF
                     ALLAFIGGLSAATGMVIVAAVSLSTMLCNDVIIPLLLRSGLVQRTGGRDLSRLLLRIR
                     RGAVVAVLLLAYLMYRALDRGFPLTQIGLVSFVAIAQLGPAFFLGLYWRRASAAGAAA
                     GIAVGFLIWAYTLLVPAMLPLTPLSAELLAQGPLGIAALRPRGLFGTGALDLISHATL
                     WSLAGNLLVFVAVSWATRQSPVERLQAAAFVSGAAAEDGSPRPRAGLARLEDLRALAA
                     RFVGAERSETAFGNYLRGRDGADAPERSGLADLDAVRFTEHLVAGAIGAPSARVVMAA
                     SLETVSLSRGAAMAMLDEASEALRFNRSLLQSTLESVPQGICTFDDELRITAWNGRFV
                     RLLGLPEPFVRVGLPLSELIAFNRDRGEYRSEDLNALLTSRDLPNQT"
BASE COUNT          476 a         1282 c         1159 g          573 t
ORIGIN      
        1 gatcggcagc gagatcagcc ccatcgagat gacggaggcc gcgacgacgc ccgtcgtcgc
       61 cgcgaggagc gcgccgacga agatgaccgc ataggcgagg cccccccgga ccgttccgaa
      121 gagctggccg atcgtgtcga ggaggtcctc cgccatcccc gagcgctcga ggatgaggcc
      181 catgaaggtg aagaacggga tcgcgagcag gacctcgttc gccatcgtgc cgtagacccg
      241 ctccggcagc gcctgcagga gcggccagga gagcgtaatg gtctgggggg cgtaaggcgc
      301 gagctcgact gcgatcagga agaacaggag gccgttcgcc gcgagcgcga aggcgaccgg
      361 gtagccggtg agcatgaaca cgaccagcgt cgcgaacatg atcggggcga ggttctgggc
      421 gatgagctcg gccatgggag gctccggagg gggtcgcgcg tcagcgcttc ggatcgacgg
      481 gcggggcgcc ggacacctcg gtcgagggct gcggcggggc gagctcggcg aggagacgct
      541 cggcctcgct ctcggcagcc gcgccgtgcc cgcctgcccc gtgatgcggg tcctcgatct
      601 cgccgcgcat caccgccacg cgcttgatga gctcgctcgc ccattgcagc aggaggacca
      661 tgaagccgag caggatcagc gccttggcgg gccagatgat cagcccaccg gcgttcgttg
      721 agatctcgcc cgagtggtac gagcgccaga agaagggcca ggacagccag atcatcacgg
      781 cgacgaaggg caggaggaag aggacgtgcc cgagcacgtc gatccagtcg cgggtgcgct
      841 tggagaagcg cgacacgacg atgtcgatgc gaatgtgctc gttgtccttg agcgtccacg
      901 cggcgcagag catgaacacg gccccgaaca ggtaccattg cagctcgagc cacgcgttgg
      961 aggaggtccc gaagaccttg cggatgatgg cgttcgccgc cgagaccacg atggcgacga
     1021 cgatggccca ggcggtgatc ttgcccaggc gcaccgtgag cgcgtcgata ccgcgactga
     1081 aggcgagcag catggcgttt cctccgccgg cgctccgggc gctcgtgcgt aggctgctcc
     1141 gtagtcgaaa cgccgtcggc gcggcccccc tactttcgct cgccccaccc cctactttgg
     1201 ctcgtttcgg cctcaattcc cggcgaacgg cagcaactct ggttgttcgg ggaagatcgc
     1261 tccgctaact tccggcgctc gcaaccgccc gttcggatcg atcttgcaca gtctcgccgt
     1321 catcgccgtc tcgggcgcct atctcggcct cctgttcggc atcgcctggt gggccgaccg
     1381 aaaggcggcc gacggctcgt tcgcgcggac cttcggaccg tgggcggcga tcaccttcgc
     1441 gctcaccctg gccatctaca acacgtcctg gagcttctac ggatcggtcg ggcgcgccgc
     1501 cgcgagcggc ctcgacttcc tgccgatcta catcggcccg acgctggtgc tgttgttcgg
     1561 ccatcgtatg ctcgccaagg tgatcgccat cgcgaaggcg cagaacgtca cctcgatctc
     1621 cgacttcatc gcggcgcgct acggcaagag tcagctcatc gcggcgctgg tcacggtcac
     1681 ggcgctcatc ggcgtgctgc cctacatcgc cctgcagctg aaggcggtcg gcacgagctt
     1741 cgacgtgctc acttcgcccg gccccgccgg aacggacgag atcgtgccgt tctggcgcga
     1801 ctcggccttc gcggtggcgc tcgcgatggc ggccttcacc atcgtgttcg gggtgcgcca
     1861 catcaacgcc agcgagcacc atcgcggtct gatggtggcg atcgccttcg agagcctcgt
     1921 caagctcggc gcgttcctcg ccgtggccgc cttcatcgtg ttcgggatgt cggacgggat
     1981 cggggcgctc ctcgaccgcg ccgcggagag cccggggctg cggcccctgc tcaccgtcga
     2041 tctcggacag ccggcgtggt tcgcgacgac gctgatcgcg gtgatcgcct tcctgtgcct
     2101 gccgcacgcc ttccacgtgg cggtggtcga gaacgacgac cctcggcacg cgcgcttcgc
     2161 cgcctggttc tatccggtct acctcgcggc cctcagcgtc ttcatggtcc cgatcgccat
     2221 cgtcgggctc accaccctcg acgcctcgat caacccggac accttcgtca tcagcctgcc
     2281 gatcgccgcc gaccggcccg gcttcgcgct cctcgccttc atcggcggtc tctcggcggc
     2341 gaccgggatg gtgatcgtcg ccgccgtgtc gctcagcacg atgctgtgca acgacgtgat
     2401 catccccctc ctgctgcgct ccgggctcgt ccagcgcacg ggcgggcgcg acctgtcgcg
     2461 cctcctgctt cggatccggc gcggcgccgt cgtcgcggtc ctcctcctcg cctacctcat
     2521 gtaccgcgcc ctcgaccgcg gcttccccct gacccagatc gggctggtct cgttcgtcgc
     2581 gatcgcccag ctcgggcccg ccttcttcct cggcctctac tggcgccggg cgagcgccgc
     2641 cggcgccgcg gcgggcatcg ccgtcggctt cctgatctgg gcctacaccc tcctcgtgcc
     2701 ggcgatgctg cccctgaccc cgctctccgc cgagcttctc gcgcaaggcc ccttggggat
     2761 cgcggcgctg cggccgcggg ggctcttcgg taccggtgcg ctcgacctca tctcgcacgc
     2821 cacgctctgg agcctcgcgg gaaacctcct cgtcttcgtg gccgtgtcct gggcgacccg
     2881 ccagagcccg gtcgagcggc tccaggcggc ggccttcgtg tccggcgccg ccgccgagga
     2941 cgggtcccct cgtcccaggg cgggcctcgc ccgcctcgag gacctgcggg cgctcgccgc
     3001 ccggttcgtc ggcgcagagc gctcggagac cgcgttcggc aactacctgc gcggccggga
     3061 cggtgcggac gccccggagc ggtccggcct cgccgatctc gacgcggtgc gcttcaccga
     3121 gcacctcgtc gcgggcgcga tcggcgcgcc ctcggcccgg gtggtcatgg ccgcctccct
     3181 cgagaccgtc tcgctctccc gcggcgccgc catggcgatg ctcgacgagg cctccgaggc
     3241 gcttcgcttc aaccgttcgc tcctgcagtc caccctcgag agcgtgccgc agggcatctg
     3301 caccttcgac gacgagctgc gcatcaccgc ctggaacggc cgcttcgttc ggctgctcgg
     3361 cctgccggaa ccgttcgtcc gcgtcggcct gcccctgtcc gagctcatcg ccttcaaccg
     3421 cgatcgcggc gagtatcgca gcgaagacct caacgcgctg ctcaccagcc gggacctgcc
     3481 gaaccagacc
//
LOCUS       DATLGG010000182         4569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_150149, whole genome shotgun sequence.
ACCESSION   DATLGG010000182 DATLGG010000000
VERSION     DATLGG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150149"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1954)
                     /gene="ftsH"
                     /locus_tag="VM638_05170"
     CDS             complement(98..1954)
                     /gene="ftsH"
                     /locus_tag="VM638_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018222220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVM11848.1"
                     /translation="MIYILLVLVGAWFFVRFATQAPEPTSITLSELESLAEQDRISTA
                     IFMERDQQVEGKLVTGEAYQTPYPQEYQDELARNLRDAGVPVSADPQKGSAVVNALLQ
                     ILPFLLIFGFIIFLMQQGQGGGGRVMSFGKAKARQVTKDEPKTSFDDVAGVDEAIEEL
                     QEVKEYLENPAKFQAMGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFV
                     EMFVGVGAARVRDLFEQAKTNAPAIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLV
                     EMDGFDARTGVILMAGTNRPDILDPALLRPGRFDRQIVIGYPDLEGRKGILRVHARGK
                     PMHESVDLDVIARQTPGFTGADLANLMNEAALLTARREKEQVTLSELEEAIDRVRLGP
                     ERKTRLISEKEKRITAYHEAGHAMVAFAHPDVDPVHKVTVIPRGRAGGYTMVLPTEDR
                     NYHTRSELLGFLAYGLGGRVAEELVIGDVTTGAQDDLRQATRTAREMVTQYGMSDALG
                     PITLGQREHEVFLGRDLNVTPDYSDQVAFEIDTEVRRLIDEAHDEALTVLQANREKLD
                     DLAGALMERETLDGEEVARILEGMTMASPKDAERRSSGIAVFRRSNGERSSD"
     gene            complement(2077..2649)
                     /gene="hpt"
                     /locus_tag="VM638_05175"
     CDS             complement(2077..2649)
                     /gene="hpt"
                     /locus_tag="VM638_05175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HVM11849.1"
                     /translation="MTEHVAEYEQDIERILISGEQIQDRIRELGERITEDYRGKDLLL
                     VGVLKGAFVVMADLSRHIRLPLQFDFMAVSSYGASTKSSGVVRILKDLDLDLEGRDVL
                     LVEDIVDSGLTLQYLLKNLRTRRPASLEVCALFQKPDVQEVEVDVRYRGFDIPPVFVV
                     GYGLDYDERYRNLPYLATLHPRVYGGSQGP"
     gene            complement(2699..3766)
                     /gene="tilS"
                     /locus_tag="VM638_05180"
     CDS             complement(2699..3766)
                     /gene="tilS"
                     /locus_tag="VM638_05180"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVM11850.1"
                     /translation="MTPRPPAVARVLERVTATVRHRGLFEEGVTVIVACSGGPDSLCL
                     LHALHRLRRLLKVRLAVFHFDHGLRRASTEDAGYVERQAARLGLPIVLRRAHDRPPGG
                     ASIEAWARTARYAALTEAAIEVGADVAATGHTRDDQAETVLIGLIRGGGLDAVAGMPP
                     RGSIPPLGIPVVRPLIDVTREETEAFCRSLRLRPRDDPHNRDPRFLRARIRTRVVPAL
                     ERAIDRGVTETLARTADAVRGDAAHLESLASEAAHAVVDVGEGEVRIVAEGVVALPGP
                     IATRVVRQALHLAGAAVGAGDAPPTSAHVAAVLDLARGRPGRRVDLVGPLSAARDRRY
                     VRLTRASPDRPPSRGARGGSR"
     gene            complement(3763..>4569)
                     /locus_tag="VM638_05185"
     CDS             complement(3763..>4569)
                     /locus_tag="VM638_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HVM11851.1"
                     /translation="IRGFSRLIEPLAPRMLRDGAGMGAAIRRKALAAQVGVLLGYVGR
                     KVLGQYDLFAEERDVLYFVGPNIVEVERRFRFSPRDFRLWLCLHEVTHRAQFDGVPWL
                     RGYLTGHIASYVRTMDLDARQLAETVRRAIEEARRSDRWRGLGFLFLLMTEEQRELFR
                     KMQALMSLLEGHANHVMARTSPGNVRGAERMRRTLQRRRRGRPLDRAVQRTLGFEAKV
                     RQYDLGEAFCLRVEQLAGQEGLGRVWEGPENLPTLEEIGDPDAWVARVVR"
BASE COUNT          649 a         1626 c         1634 g          660 t
ORIGIN      
        1 cttcagcccc tcgcgcccga catcctctcc gaccccttcg aggatcaggc gaacgccctc
       61 ctcgaccttc tgcgggtcga actccgccac ggcggcctca gtccgacgag cgctcgccgt
      121 tggagcggcg gaagacggcg atcccggagc tgcgacgctc ggcgtccttg ggcgaagcca
      181 tcgtcatccc ctcgaggatc cgggcgacct cctcgccgtc gagggtctcc cgctccatga
      241 gcgcacccgc gaggtcgtcg agcttctccc ggttggcctg caggaccgtc agcgcctcgt
      301 cgtgcgcctc gtcgatcagg cggcgcacct cggtgtcgat ctcgaacgcg acctggtcgg
      361 agtagtccgg ggtgacgttg aggtcgcggc cgaggaagac ctcgtgctcc cgctggccga
      421 gcgtgatcgg accgagcgcg tccgacatgc cgtattgcgt gaccatctcg cgcgcggtgc
      481 gggtggcctg ccggaggtcg tcctgcgccc cggtggtgac gtcgccgatc acgagctcct
      541 cggcgacacg cccaccgagc ccataggcga ggaacccgag cagctccgag cgcgtgtggt
      601 agttccgatc ttcggtcggc agcaccatcg tgtacccacc ggctcgcccg cgcgggatca
      661 ccgtgacctt atgcacgggg tccacgtcgg ggtgcgcgaa ggcgaccatc gcgtgaccgg
      721 cctcgtggta cgccgtgatg cgcttctcct tctcggagat caggcgcgtc ttccgctcgg
      781 ggccgaggcg gacgcggtcg atcgcctcct cgagctccga gagcgtgacc tgctccttct
      841 cccggcgagc ggtcaggagc gccgcctcgt tcatcaggtt ggcgaggtcg gctccggtga
      901 agccgggggt ctggcgcgcg atcacatcga ggtcgacgct ctcgtgcatc ggcttgccgc
      961 gcgcgtgcac gcgcaggatg cccttgcggc cctcgaggtc gggatagccg atcacgatct
     1021 ggcggtcgaa gcggccgggc cgcaggagtg ccgggtcgag gatgtccggg cggttggtgc
     1081 cggccatgag gatcacgccg gtgcgcgcgt cgaagccgtc catctcgacg aggagctggt
     1141 tcagggtctg ctcgcgttcg tcgtggccgc cgccgagccc cgcgccgcgc tggcgtccga
     1201 cggcgtcgat ctcgtcgatg aacacgatgg ctggggcatt cgtcttcgcc tgctcgaaga
     1261 ggtcccggac gcgcgcggcg cccacgccga cgaacatctc cacgaagtcc gagcccgaga
     1321 tcgagaagaa gggaaccccg gcctcaccgg cgacggcgcg ggcgagcagg gtcttcccgg
     1381 ttcccggcgg cccgtacagc aggacgccct tggggatctt cgcccccatc gcctggaact
     1441 tcgccgggtt ctcgaggtac tccttcacct cctgcagctc ctcgatcgct tcgtcgaccc
     1501 ccgcgacgtc gtcgaaggag gtcttcggct cgtccttcgt gacctgccgc gccttcgcct
     1561 tgccgaacga catcacgcgg ccgccgccac cctggccctg ctgcatcagg aagatgatga
     1621 agccgaagat caggaggaac ggcaggatct gcaggagggc gttcacgacc gccgagccct
     1681 tctgcgggtc agcgctgacg ggcacgccgg cgtcgcgcag gttgcgtgcg agctcgtcct
     1741 ggtactcctg cgggtacggg gtctggtagg cctcgcccgt gacgagcttc ccctcgacct
     1801 gctgatcgcg ctccatgaag atggccgtcg agatgcggtc ctgctcggcc agggactcga
     1861 gctccgagag cgtgatggag gtcggctccg gcgcctgcgt cgcgaagcgg acgaagaacc
     1921 aggccccgac cagaacgagc aggatgtaga tcaacgggcc acggaacgcc cgtcctgccc
     1981 ggggggtgcg agggttcagc gaggctctcc tttatcgggg tcggccgccg gcagtggtcg
     2041 cggcagtctc gaaccgatgg tagcacgcgc ccctgactag gggccctgcg agcccccgta
     2101 gacgcgcggg tgcagcgtgg cgaggtacgg caggttgcgg tagcgctcgt cgtagtcgag
     2161 cccgtacccg acgacgaaga cgggggggat gtcgaatccg cggtaccgca cgtcgacctc
     2221 gacctcctgg acgtcgggct tctggaacag ggcgcagacc tcgaggctcg ccggccggcg
     2281 ggtccgcagg ttcttcagca ggtactggag cgtcaagccc gagtcgacga tgtcctcgac
     2341 gaggaggacg tcgcggcctt cgaggtcgag gtcgaggtcc ttgaggatcc ggacgacgcc
     2401 ggacgacttc gtcgaggccc cgtaggagga gaccgccatg aagtcgaact gcagcggcag
     2461 gcggatgtga cgcgagaggt cggccatgac cacgaaggcc cccttgagga cgccgaccag
     2521 caggagatcc ttgccgcggt agtcctcggt gatccgctcc ccgagctcgc ggatgcggtc
     2581 ctggatctgc tcgccgctga tgaggatgcg ctcgatgtcc tgctcgtact cggcgacgtg
     2641 ctcggtcaac gcggtgctcc ggccgttccc ctcgccctgc acgacgtctc ccccttcctc
     2701 agcgagatcc tccacgggca ccacgcgacg gcggtcggtc gggggaagcg cgagtgaggc
     2761 gaacataccg cctgtcgcgc gccgcggaca aggggccgac gaggtccacg cgccgtcccg
     2821 gacgtccccg cgcgaggtcg aggacggcgg cgacgtgggc cgaagtgggc ggggcgtctc
     2881 cggcgccgac ggccgcgccc gcgaggtgga gcgcctgacg gaccacgcgc gtcgcgatcg
     2941 gcccgggaag cgccacgacc ccctcggcca cgatgcggac ctcgccctca ccgacgtcca
     3001 ccaccgcgtg cgccgcttcg gaggcgagcg actcgagatg ggccgcgtcg ccgcggaccg
     3061 cgtcggcggt ccgggcgagg gtctcggtga ccccgcggtc gatcgcccgc tcgagcgcgg
     3121 gcaccacccg ggtgcggatc cgggccctca ggaagcgcgg gtcgcggttg tgggggtcgt
     3181 cacgcggccg cagccggagc gagcggcaga aggcctccgt ctcctcgcgc gtcacgtcga
     3241 tcaacggccg gaccaccggg atgccgagtg gggggatcga cccgcgcggg ggcatccccg
     3301 cgaccgcgtc gagtccgccg ccgcggatca gcccgatcag cacggtctcg gcctgatcgt
     3361 cgcgggtgtg cccggtcgcc gcgacgtcgg cgccgacctc gatcgccgcc tccgtcagcg
     3421 ccgcgtagcg ggcggtccgg gcccacgcct cgatcgatgc gccgccgggc ggacggtcgt
     3481 gagcccgccg gaggacgatc gggagcccga ggcgggccgc ctgccgctcg acgtacccgg
     3541 cgtcctccgt ggaggcgcgg cggagcccgt ggtcgaagtg gaagaccgcc aagcgcacct
     3601 tcagcagccg gcgaaggcgg tggagggcgt gcagcaggca gagggagtcc gggccgccgg
     3661 aacacgcgac gatcacggtg acgccctcct cgaagaggcc gcggtgccgg accgtggcgg
     3721 tcacgcgctc gaggacccgc gcgaccgccg gggggcgggg ggtcaccgca cgacccgcgc
     3781 cacccacgcg tccgggtccc cgatctcctc gagggtcggc aggttctccg gcccctccca
     3841 gacgcggccg agcccttcct gcccggcgag ctgctcgacg cgcaggcaga aggcctcccc
     3901 caggtcgtac tggcggacct tcgcctcgaa ccccagggtt cgctggaccg cccggtccag
     3961 cggacgtccc cgacgacgtc gttggagcgt gcgtcgcatc cgctcggcgc cccggacgtt
     4021 ccccggggag gtccgcgcca tcacgtggtt cgcgtgaccc tcgaggagcg acatcagcgc
     4081 ctgcatcttc cggaagagct cgcgctgctc ctcggtcatc agcaggaaca ggaagccgag
     4141 accccgccat cggtcgctgc gacgcgcctc ctcgatcgcc cgccggacgg tctcggcgag
     4201 ctggcgggcg tcgagatcca tcgtgcgcac gtacgaggcg atgtgacccg tgaggtagcc
     4261 gcgcagccag gggacgccgt cgaactgcgc gcggtgcgtc acctcgtgca gacagagcca
     4321 gagccggaag tcccgcggtg agaaccggaa ccggcgctcg acctcgacga tgttcggccc
     4381 cacgaagtac aggacgtcgc gctcctccgc gaagaggtcg tactggccga ggaccttgcg
     4441 gccgacgtag ccgaggagca ccccgacctg cgccgcgagc gccttccggc ggatcgccgc
     4501 gcccatgcct gccccgtcgc ggagcatccg cggcgcgagc ggctcgatca ggcgggagaa
     4561 cccgcggat
//
LOCUS       DATLGG010000183        14899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_71052, whole genome shotgun sequence.
ACCESSION   DATLGG010000183 DATLGG010000000
VERSION     DATLGG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14899
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71052"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VM638_05190"
     CDS             <1..350
                     /locus_tag="VM638_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVM11852.1"
                     /translation="YGRAGYREMVERHLDLAQRVGRRVEEADDLELLAEVKLNIVCFR
                     YRPPGVDDEEAIDEINRRIGQEILRDGRVYVGTTTYDGKVAFRPAIVNWRTREEDVDL
                     LVDTIRDLGSRPA"
     gene            complement(360..995)
                     /locus_tag="VM638_05195"
     CDS             complement(360..995)
                     /locus_tag="VM638_05195"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVM11853.1"
                     /translation="MRVKAGSGRDGSGMVTTKRRMRGPERRAQLLSVARSVFARDGYH
                     GVTMDDVAAEAGVTKPILYDHFPSKRELYLALLEADLAELRERVHAGLQTSTGNRERI
                     RSSIRAYVEFVDEHAEGFQLLMQEAIGAEREFRELIGTFKDEVRAEVADVIARESKGK
                     VAREDADVVAQALIGMTETVAQQGIGRSKEERDRVVNTLVRLAWRGITGLA"
     gene            1074..2261
                     /locus_tag="VM638_05200"
     CDS             1074..2261
                     /locus_tag="VM638_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM11854.1"
                     /translation="MDFRPSEELVTLQKWAHEFAEKEIRPVAPEYDESDEFPWPVVKK
                     AAEVGLYSLEFYTETVMPDPTGVALLMAAEETTWGCAGIALALFGTGLPLASLAAAGT
                     PEQLQRWAPEMFGTPAEPKLGAFAVTEPNAGSDVSALRTTARREGDEWVLNGQKVFIT
                     NGGIADVHIVVATVDPELGHRGQAAFIVPKVPGITQGKKERKLGIRASHTAEVLLDDC
                     RIPLENVLGGMDRLEQKLERARRQEHTRTSHALKTFEMTRPLVAASAIGVARAAYEFA
                     LEYAKERTQFGRPIIENQGIAFKLADMAMEIDAARLLTHRAGWMARNGVDFTRAEGSM
                     SKLKASEVAVWVTEQAIQVLGGYGYIKDFPVEKWYRDSKIFTLFEGTSEIQRLVISRA
                     AAR"
     gene            2303..3265
                     /gene="corA"
                     /locus_tag="VM638_05205"
     CDS             2303..3265
                     /gene="corA"
                     /locus_tag="VM638_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HVM11855.1"
                     /translation="MITCRLYRDGRLAEEGSDPRLVSDRLAEPEGLIWLDAADPTDEE
                     LRLIEEEFGLHPLAVEDARHRNQRPKLESYEAYDFLVAHEVTFDGVEVANAELHAFVA
                     PRFLVTLRFPPVGDLRRALAWANRHPDLVREGGGALLYALLDRVVDGYFLVVDAFEDR
                     TDEVESEVFAEDTNDDVQRDIFRLKKQVIEFRRLAAPLREVLNLVQATPAIVTPPLTA
                     YYRDVADHLIRTLEFVDNVRELLGTALDAHLSQVSNRLNEVMKAVTSWGAIILVPTLI
                     AGIYGMNFRHMPELSWLLGYPAALGLMALSAGALFVAFKRRGWL"
     gene            complement(3295..3918)
                     /locus_tag="VM638_05210"
     CDS             complement(3295..3918)
                     /locus_tag="VM638_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVM11856.1"
                     /translation="MRRHPHSRRVLAATIGGALLFSLTAPATAHRPKHWRYEKRIRNH
                     KAWQDHQRFRVLHRRWHEERLATVSEDVPQEDGTTLTVTRTEPTYTEAEHRRFHHRLR
                     HRHLKRHHRYRVVARQRGRASWYDYEGRVGACGKVLKGMYAAHRTWPCGSKVAVKRGG
                     RVVVVRVLDRGPFVDGWVIDLAPRAFRKVGGLGEGVVPVKIFRLKKR"
     gene            4181..5134
                     /locus_tag="VM638_05215"
     CDS             4181..5134
                     /locus_tag="VM638_05215"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018740339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HVM11857.1"
                     /translation="MPKRILLAWPRGYCAGVDRAVEVVERALRIYGAPVYVRKQIVHN
                     IHVVRDLERKGAVFVEEETEVPEGATVVLSAHGVAPTVYANSEARELRVIDATCPLVT
                     KVHLEARRFAADGYTILLIGHEGHEEVVGTSGEAPEHIRLVGDPQEAREVDVPNPGRV
                     AYLSQTTLSVDETNEVIEVLRERFPALQGPPRDDICYATQNRQEAVKALAKQAELVLV
                     IGSDNSSNSNRLVEVARSEGAASYLIDDETEVDPAWLEGVETVGVTSGASAPEWLVER
                     VVGHLRNLGASEVEELRIREEHVVFGLPPGVREADAAAARS"
     gene            complement(5162..6358)
                     /locus_tag="VM638_05220"
     CDS             complement(5162..6358)
                     /locus_tag="VM638_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HVM11858.1"
                     /translation="MDEQPRKNVKDLLAESKDTSELMVDLAYAAVFFNDEELAGEVER
                     LEEMLDAYLADLRWISMLAARSPEDAEAMGGVLHIAGAMEKIGDAASDIARVVAAKLG
                     LPDALRQDLRHADEIVGRVKVREGAECVGRTLRELMLPIETGMWLIAVRRGVEWEFDP
                     GPETVISDGDVLLFQGPEEGVNLIREQAGAPPVPGPPEADALPLTELDRAVDILVEMK
                     NSAEVAVGLAYSSLLLGEAALAAEVATLESRSDMLHDQLESWVLRASPEARNPDELRG
                     LIRLAYATEAIFDAAREMTRLVERGEELHPVVAAAIQASDEVSAEAIVEEGAPALGKT
                     LKQLSVETETGMMVLAVQRGRRWIYRPRPMFELQVDDRLIASGPEDGIAELNDLVRAP
                     QAATAG"
     gene            complement(6387..7607)
                     /locus_tag="VM638_05225"
     CDS             complement(6387..7607)
                     /locus_tag="VM638_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HVM11859.1"
                     /translation="MRGRVGAVFAYWRAERRTIRQGFVALVVASTGNLVAGLTLGSIT
                     GTLERLPGLMVLVPAAIGMRGNIFGALGSRLGTTIHTGLFEASWRRQGVVYQSVSAAV
                     VLTFAISVVLAVLAKGLSEVFGFASISVVDLVAISLIGGVLSSVVVGIFTVALSVGAF
                     RRGWDLDSVAAPLVTAVGDMVTLPALFLATFIVGIPIVTPAVTAAMVLVTLVLTIQGV
                     RTNLQITRRVVRQGILLLSVAGGIDVLAGLVAESRLEHFITYPALLVLIPPFLADAGA
                     LGGILSARLASKLHLGALTPRGRPEPVALLDTSIIFLFAIWLFALVGISADIVAGLFG
                     LASPGALTIVGVSLFGGLLATCFAVVVAYYAATASFRLGLDPDDFGIPIITSSMDFLG
                     VISLVIALIVFGVA"
     gene            7780..7995
                     /locus_tag="VM638_05230"
     CDS             7780..7995
                     /locus_tag="VM638_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11860.1"
                     /translation="MKTKTIWFALFLLYFGLLALPAQAYLDPGTGSVIFQVIVGGLLG
                     AAFAIKTFWRRIVGFFSRRKPESERVD"
     gene            7988..9364
                     /locus_tag="VM638_05235"
     CDS             7988..9364
                     /locus_tag="VM638_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVM11861.1"
                     /translation="MTDPDVLPSSFRDPSGFVFRREGILYRQVNRSFQESFDALLSSG
                     LYDALTGDGLLIPHEEAGEPATDEAYRVLRPEPIPFVSFPYEWSFSQLKDAALATLRI
                     QSLALDHGMTLRDASAYNIQFRDGRPIFIDTLSFEPWTEGQPWVAYRQFCQHFLAPLA
                     LAAFADIRLLQLLRTNIDGVPLDLAARLLPGAARRRLGIRLHVVAHAKSQRRHQGDAE
                     APSRGRSFSMRAFRGLLDNLTSTVRALSWSPGRTTWSSYYEEADHYSDEAFERKRSLV
                     DRFIGETSPASVWDLGGNIGVFARIASERGIPTVCFDLDEAAVEANYRRVREQAERQL
                     LPLVLDLTNPSPALGWAGAERPSLAQRGPVDLALALALVHHLAIGNNVPLQRIAAYFA
                     DLCEGLVIEFVPKEDEKVRTLLATREDVFPDYTEEGFERAFEERFAIGSKEPIEGSAR
                     TLYLMKRR"
     gene            9364..10869
                     /locus_tag="VM638_05240"
     CDS             9364..10869
                     /locus_tag="VM638_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVM11862.1"
                     /translation="MRWPRPLVLHPLLFAALPVVYLYAANLAETNLSDVLAPLAVILV
                     GAVILLAAAAFLLRRVHAAGVAVSGLILLFFSYGHVHRGLAGTPVGRHRVLLVLWAAL
                     ALALVAAVRGRRWPVRATGALNFVAAGLVLVNVATIALYNLRSRPAEVGDPGIAAAPR
                     TAPDRDIYYLVFDRYGGERALRGSFRFDNSTFVEGLEKRGFYVADRSSGNYPRTLHSL
                     ASSLNMDHLLELARRVGEDSGDGGPLREILADHRVGRFLKEAGYRYIQVGSWWAPTAT
                     SPLADRTMRAGLLSEFSAVLLSTTAAAPVLEMVSGEMEPRRREYNRTLQQFENLTKAS
                     RLPGPKFVFAHFLVPHTPYVFDRNGAFLPQEIHDARSEGENYVEQLRYVNRRIEQLLD
                     EILAVPEKERPIVLLQSDEGPGERPESWEGVPQRVLEEKFFILNAYHLPGVEDPPLYP
                     TITPVNSFRLVLNLYFGAELPLLPDRVYVFQDIDHLYRFTDVTERLFGDDP"
     gene            complement(10885..12219)
                     /gene="gabT"
                     /locus_tag="VM638_05245"
     CDS             complement(10885..12219)
                     /gene="gabT"
                     /locus_tag="VM638_05245"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HVM11863.1"
                     /translation="MSIPQQRRLVTEIPGPRSKELLDRRALAVPPAVFNTVPVFVQDA
                     DGAVLEDVDGNRLIDLGAGLAVLNVGNAAPEVVEAVRRQAEAFTHTCFHVTMHEPYVE
                     LAERLNELTPGEHAKQTMLVNSGAEAVENAVKIARHATGRAGVVAFDHAFHGRTLLAM
                     TLTAKDMPYKHGFGPFAPDVHRLPYAYPYRCPAGASPQECGPACAERAIDRMRELGPG
                     NIACLVVEPVQGEGGFVVPGEGFLEALHAFCREEGIVFIADEVQTGFARTGRWFGIEH
                     SGLVPDLVVSAKGLGGGLPIGAVTGRRELMEGVHVGGLGGTFGGNPIACAAALAVIDL
                     IERDDLCARAERIGERMRGRLETAATETDLIGDVRGLGAMAAVELVDDPDTKEPAKQA
                     AARVIEECYRNGVVVLKAGTYDNVVRLLPPLTIDEELLDEALGVLAESLVAR"
     gene            12366..13919
                     /locus_tag="VM638_05250"
     CDS             12366..13919
                     /locus_tag="VM638_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVM11864.1"
                     /translation="MAGVRVADIVELDSLGLNLVSGRRGARHPIRWVHVSELEDPTPW
                     LKGGELLLTTGMGLGKSASRQRAYIERLASAGLAGLGFGIGFTYRRVPKAIVDASERL
                     SFPVFEVPYPVPFIAITEAVYSRLSAEQVDVLRRALEAQGALTRAVLAGDGVAGVVAA
                     LRGATGGWALALDVRGGVVAASPSAVASRAPRIWEEVRAARPSGPGFALSVNDRDDHV
                     SIHPIGVHGHVEGYLATGAAHPPGQLERIVGGHALSLLALELDKTRALADAERRLRGD
                     LFDALARGELSESEARRGLRRFGFDRADAVAVVAIEAEAPMQDLAWAAEDLLALDGVP
                     FLTAPRDRTLFVLLRPNGRGTLEDLHRRLSERLPEGVLAAAGSAVEPAEAARSLREAR
                     YALEVCRAQGRSFADVHALGTYRLLLTLQEPDALRAFADAVLTPLDEYDRSGEGELVP
                     SLQAFLEHNARWESAAAALFVHRHTLRYRMRKVEELTGRDLSSAHDRMEFWLALRARE
                     MVARETEEV"
     gene            14024..>14899
                     /locus_tag="VM638_05255"
     CDS             14024..>14899
                     /locus_tag="VM638_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM11865.1"
                     /translation="MTTKQMFIGGDWVDGSGGSVEVINPATGERLAETPNATVEDVDR
                     AVTAAQRAFDDVWFDSTPAERQAMLLKWADRIEEHAEELGRIESENVGKPMSWVVSDE
                     ILVVAEQIRFFAGAARVMGGLSAGEYFKDDSTMYTSWLRREPIGVVGLIAPWNYPLYM
                     AMWKFAPALAAGNTVVIKPSEWTPLTLLRATELAADIFPPGVINVVTGEGQPTGAAIV
                     EHPGVGMVSLTGEVTTGKEVARAAASTLKRVHLELGGKAPVVVFDDADPAAVAEGIKV
                     AGYWNSGQDCTAASRV"
BASE COUNT         2152 a         5266 c         5279 g         2202 t
ORIGIN      
        1 cctatgggcg ggcggggtac cgcgagatgg tcgagcgcca cctcgacctc gcccagcggg
       61 tgggccgccg ggtcgaggag gccgacgatc tcgagctcct cgcggaggtg aagctcaaca
      121 tcgtctgctt ccggtaccgc ccgccgggcg tcgacgacga ggaggcgatc gacgagatca
      181 accgtcggat cgggcaggag atcctccgcg acggccgcgt ctacgtcggc accacgacct
      241 acgacggcaa ggtcgcgttc cgccccgcca tcgtcaactg gcgcacgcgc gaggaggacg
      301 tcgacctcct cgtcgacacg atccgcgacc tcggctcccg tccggcgtag tccttcggct
      361 caggcgaggc cggtgatgcc tcgccaggcg aggcgaacga gggtgttgac gacgcggtcg
      421 cgctcctcct tcgagcgacc gatgccctgc tgagcgacgg tctcggtcat cccgatcagc
      481 gcttgggcga cgacgtcggc gtcctcgcgg gcgaccttcc ccttcgactc gcgcgcgatg
      541 acgtcggcga cctccgcgcg cacctcgtcc ttgaaggtcc cgatcagctc ccggaactcg
      601 cgctcggccc cgatcgcctc ctgcatcagg agctggaagc cttcggcgtg ttcgtcgacg
      661 aactccacgt aggcgcggat cgagctgcgg atccgctcgc ggttgccggt cgaggtctga
      721 agccccgcgt ggacgcgctc gcgcagctcc gcgaggtcgg cttcgaggag cgccaggtac
      781 agctcgcgct tcgaggggaa gtggtcgtag aggatcggct tcgtgacgcc ggcttccgcg
      841 gcgacgtcgt ccatcgtgac gccgtggtag ccgtcgcggg cgaagacgga gcgggccacg
      901 ctcaggagct gcgcccgccg ctccggccct cgcatccgtc gcttcgtcgt gaccatcccg
      961 gatccgtccc tccccgagcc tgccttgacg cgcatcctac cgcatagtaa cctaccggct
     1021 ggtagggagc cgtccgggac gggacggccc ggcacattct ctcaggaggc acgatggatt
     1081 tccgaccctc cgaggagctg gtcacgctcc agaagtgggc gcacgagttc gccgagaagg
     1141 agatccgccc ggtggcgccc gagtacgacg agtccgacga gttcccgtgg ccggtcgtga
     1201 agaaggccgc cgaggtcggg ctctactcgc tcgagttcta caccgagacc gtgatgccgg
     1261 acccgaccgg cgtggccctc ctgatggcgg ccgaggagac cacgtggggc tgcgcgggga
     1321 tcgcgctggc cctgttcggc acggggcttc cgctcgcctc gctggccgcc gccgggacgc
     1381 ccgagcagct ccagcgctgg gcccccgaga tgttcggaac gcccgccgag ccgaagctcg
     1441 gggcgttcgc ggtcaccgag ccgaacgccg gctccgacgt ctcggcgctg cgcaccaccg
     1501 cgcggcgcga gggcgacgag tgggtcctga acgggcagaa ggtcttcatc acgaacggcg
     1561 ggatcgccga cgtgcatatc gtcgtcgcca ccgtcgaccc ggagctcggc caccgcggtc
     1621 aggcggcgtt catcgtcccg aaggttcccg gcatcaccca gggcaagaag gagcgcaagc
     1681 tcgggatccg ggcctcgcac accgccgagg tgctcctcga cgactgccgg atcccgctcg
     1741 agaacgtcct gggcgggatg gaccgcctcg agcagaagct cgagcgcgcg cgccggcagg
     1801 agcacacgcg gacctcgcat gcgctcaaga ccttcgagat gacccgtccc ctcgtggcgg
     1861 cctccgcgat cggcgtcgcg cgggcggcgt acgagttcgc gctcgagtac gcgaaggagc
     1921 gaacgcagtt cgggcggccg atcatcgaga accaggggat cgcgttcaag ctcgccgaca
     1981 tggcgatgga gatcgacgcg gcacgcctgc tcacgcaccg cgcggggtgg atggcgcgca
     2041 acggcgtgga cttcacccgc gccgaagggt cgatgtcgaa gctcaaggcg agcgaggtcg
     2101 cggtctgggt caccgagcag gcgatccagg tcctcggggg ctacgggtac atcaaggatt
     2161 tcccggtcga gaagtggtac cgcgactcga agatcttcac gctgttcgag ggcacgagcg
     2221 agatccagcg gctcgtgatc agccgggccg ccgcccgctg acctctcccg gccggcgcgc
     2281 gtccctgcgc tagagtcgcc gcgtgatcac ctgccggctc taccgcgacg gtcgtctggc
     2341 cgaggaaggg tcggatccgc ggctcgtcag cgatcgtctc gccgagcccg aagggctcat
     2401 ctggctcgac gcggcggacc cgaccgacga ggagctgcgg ctcatcgagg aggagttcgg
     2461 cctgcacccg ctcgccgtcg aggatgcgcg ccaccgcaac cagcgtccga agctcgagtc
     2521 gtacgaggcc tacgacttcc tcgtcgcgca cgaggtgacc ttcgacggtg tggaggtggc
     2581 caacgcggag ctccacgcgt tcgtggcccc gcgcttcctc gtgacgctgc ggttcccgcc
     2641 ggtgggggac cttcggcggg cgctcgcctg ggcgaaccgg caccccgacc tggtgcgcga
     2701 gggcggcgga gcgctgctct acgcgctgct cgaccgggtc gtcgacggct acttcctcgt
     2761 ggtcgacgcg ttcgaggacc gcaccgacga ggtggaatca gaggtcttcg ccgaagacac
     2821 gaacgacgac gtccagcggg acatcttccg gctgaagaag caggtcatcg agttccgccg
     2881 gctcgcggcg cccctgcgtg aggtcctcaa cctcgtccag gcgacgccgg cgatcgtgac
     2941 cccgccgctg acggcgtact accgcgacgt ggccgaccac ctgatccgaa cgctcgagtt
     3001 cgtcgacaac gtgcgtgagc tcctcgggac ggcgctcgac gcgcacctct cccaggtgag
     3061 caaccgcctc aacgaggtga tgaaggcggt gacctcctgg ggcgcgatca tcctcgtgcc
     3121 gacgctgatc gcggggatct acggcatgaa cttccgccac atgccggagc tgagctggct
     3181 cctcggctat cccgccgcac tcgggctgat ggcgctctcc gcaggcgccc tgttcgtggc
     3241 gttcaagcgg cgggggtggc tctagccgcg cgcggccgag ccaccccggc cggctcaccg
     3301 cttcttcaga cggaagatct tcaccggcac gacgccctcg ccgaggccgc cgaccttccg
     3361 gaaggcgcgc ggtgcgaggt cgatgaccca gccgtccacg aacggcccgc ggtccaggac
     3421 ccgcacgacg accacgcggc cgccacgctt caccgcgacc ttcgacccgc acggccaggt
     3481 ccggtgggcg gcgtacatcc ccttcagcac cttcccgcag gcgccgacgc gcccttcgta
     3541 gtcgtaccaa gaggcacggc cgcgctgccg cgcgaccacc cggtagcggt ggtggcgctt
     3601 gagatgacgg tgccgcagcc ggtggtggaa ccgccggtgc tccgcctcgg tgtaggtcgg
     3661 ctccgtccgc gtgaccgtga gggtcgtgcc gtcttcctgc ggcacgtctt cgctgacggt
     3721 cgccaggcgt tcctcgtgcc agcggcgatg caggacgcgg aaacgctgat ggtcctgcca
     3781 ggccttgtgg ttgcggatgc gcttctcgta gcgccagtgc ttcggtcggt gggcggtggc
     3841 gggggcggtg agggagaaca ggagcgcccc gccgatcgtc gccgcgagga cgcggcggct
     3901 gtgcggatgt cgtctcatat cggatgcctt cacccccacg gctcaggccg gggttatccg
     3961 cccccggtcg cggccgcggt aggtcctggg tgctggcttc acctcctcgt ggtcgatggg
     4021 cgtgctccca ccccgttcgg gcgcgttcca gcgcctggag gagtgggatc ggaggacccc
     4081 tacccatccg ggagcgggat atgcaccttc gtcggaagat cttccgacgg cccggcggcc
     4141 gacccaccgg tcatagggag gcaggcggta cgatggaggc gtgccgaaga ggatcctgct
     4201 cgcgtggccg aggggctact gcgccggtgt ggaccgcgcc gtcgaggtgg tcgagcgggc
     4261 gctgcgcatc tacggcgccc cggtctacgt gcgcaagcag atcgtgcaca acatccacgt
     4321 cgtccgcgac ctcgagcgga agggcgccgt cttcgtcgag gaggagaccg aggtgcccga
     4381 gggggcgacg gtcgtgctct ccgcgcacgg ggtcgcgccg acggtctacg cgaactcgga
     4441 ggcgcgcgag ctgcgggtga tcgatgcgac ctgtccgctc gtgacgaagg tccacctcga
     4501 ggcgcgccgg ttcgccgccg acggctacac gatcctgctg atcggccatg agggccacga
     4561 ggaggtcgtc gggacgagcg gcgaggcgcc ggagcacatc cgcctcgtcg gggacccgca
     4621 ggaggcgcgc gaggtcgacg tcccgaaccc cggtcgggtc gcgtacctgt cgcagacgac
     4681 gctttcggtc gacgagacga acgaggtgat cgaggtcctc cgcgagcgct tcccggcgct
     4741 gcagggaccg ccgcgcgacg acatctgcta cgccacgcag aaccgccagg aggccgtgaa
     4801 ggcgctcgcg aagcaggcgg agctcgtcct cgtgatcggc tccgacaact catcgaactc
     4861 gaaccgcctc gtcgaggtcg ctcgctccga gggcgcggcg tcctacctga tcgacgacga
     4921 gaccgaggtc gacccggcgt ggctcgaggg cgtcgagacg gtcggcgtca cctctggggc
     4981 gtctgccccg gagtggctcg tcgagcgcgt cgtcggccac ctccgcaacc tcggcgccag
     5041 cgaggtcgag gagctgcgga tccgcgagga gcacgtcgtc ttcgggcttc cgccgggcgt
     5101 ccgcgaagcc gacgccgcgg ccgcccgctc ctgagggcga tccggcccgc cggccgcgtg
     5161 ctcagccggc ggtcgcggct tgcggggcgc ggaccaggtc gttgagctcg gcgatcccgt
     5221 cctccggccc gctggcgatc aggcggtcgt cgacctggag ctcgaacatc ggccgcgggc
     5281 ggtagatcca ccggcggccg cgctggacag cgaggaccat catcccggtc tcggtctcca
     5341 ccgagagctg cttcaacgtc ttgccgaggg ccggcgcgcc ctcctcgacg atcgcctcgg
     5401 ccgagacctc gtcgctggcc tggatcgcgg ccgcgacgac cgggtggagc tcctcgcccc
     5461 gctcgacgag gcgcgtcatc tcgcgcgcgg cgtcgaagat cgcctcggtt gcgtacgcga
     5521 gccggatgag accgcgcagc tcgtcggggt tccgagcctc gggcgaggcg cgcaggaccc
     5581 aggactcgag ctggtcgtgc agcatgtcgc tccgggattc gagggtggcc acctcggccg
     5641 ccagcgccgc ctcgccgagc agcagcgagg agtaggcgag accgaccgcg acctcggcgg
     5701 agttcttcat ctcgacgagg atgtcgaccg cgcggtcgag ctcggtcagg ggcagcgcgt
     5761 ccgcctcggg cgggcccggc accggtggcg cgcccgcctg ctcgcggatc aggttgacgc
     5821 cctcctcggg gccctggaac aggagcacgt cgccgtcgga gatcaccgtc tcgggcccgg
     5881 ggtcgaactc ccactcgacc ccgcgccgca cggcgatgag ccacatcccg gtctcgatcg
     5941 gcagcatcag ctcgcggagg gtgcgaccga cgcactccgc gccctcgcgg accttgacgc
     6001 ggccgacgat ctcatcggcg tgccgcaggt cctggcgcag cgcgtcgggg agcccgagct
     6061 tggccgcgac gacccgcgcg atgtcggagg cggcgtcgcc gatcttctcc atcgccccgg
     6121 cgatgtgcag gacgccgccc atcgcctcgg cgtcctccgg gctgcgcgcc gcgagcatcg
     6181 agatccagcg gaggtcggcg aggtaggcgt cgagcatctc ctcgagccgt tcgacctcgc
     6241 cggcgagctc ctcgtcgttg aagaagaccg ccgcgtaggc gaggtcgacc atcagctcgg
     6301 aggtgtcctt cgactccgcg aggaggtcct tgacgttctt cctgggttgt tcgtccatca
     6361 gggatcgacg cctccgagta taggggtcat gccacgccga acacgatgag ggcgatcacg
     6421 agcgagatca cgccgaggaa gtccatcgag ctcgtgatga tcgggatgcc gaagtcatcg
     6481 gggtcgaggc cgaggcggaa gctcgcggtg gccgcgtagt aggcgacgac gacggcgaag
     6541 caggtcgcca ggagcccccc gaacaggctg accccgacga tcgtcagcgc tcccgggctc
     6601 gccagcccga acagccctgc gacgatgtcc gcggagatcc cgaccagcgc gaacagccag
     6661 atcgcgaaca ggaagatgat cgaggtgtcg aggagcgcca ccggctccgg acgtccccgc
     6721 ggcgtcagcg cgccgaggtg gagcttcgag gccagccgtg ccgagaggat cccgccgagc
     6781 gcgccggcgt cggcgaggaa cggcgggatg aggacgagga gcgccggata cgtgatgaag
     6841 tgctccagcc gcgactcggc gacgaggccc gcgaggacgt cgatgccgcc cgcgaccgag
     6901 agcaggagga tcccctgccg cacgacgcgc cgggtgatct gcaggttggt ccgcacgccc
     6961 tggatcgtca gcacgagcgt cacgagcacc atcgccgcgg tcacggccgg ggtcacgatc
     7021 gggatcccga cgatgaaggt ggcgaggaac agcgcgggca acgtgaccat gtcgccgacg
     7081 gcggtcacga ggggcgcggc gacgctgtcg aggtcccagc cccgccggaa cgcgccgacg
     7141 gagagcgcga ccgtgaagat gccgaccacc acgctcgaga ggacgccgcc gatgagcgag
     7201 atcgcgacga ggtccaccac cgagatgctc gcgaacccga agacctccga cagccccttc
     7261 gcgaggacgg ccaggacgac cgagatcgcg aacgtcagga cgacggcggc cgacacgctc
     7321 tggtagacga cgccctgccg acgccagctc gcctcgaaca ggcccgtgtg gatcgtcgtg
     7381 cccagcctcg agccgagcgc gccgaagatg ttgccccgca tcccgatggc ggcggggacg
     7441 aggaccatca ggcccgggag ccgctcgagc gtgccggtga tcgagccgag cgtcagtccg
     7501 gcgacgaggt tgccggtcga ggcgacgacg agcgcgacga acccctgccg gatcgtccgg
     7561 cgctcggcgc gccagtacgc gaagaccgca ccgaccctgc cccgcatctc ggcaccacca
     7621 tcgcgtcgga cctcctcggc tcgtcctgct cagtatcgcc cgcgggcgcg gaaggcctca
     7681 agcgcatacc gaacccggct cgagggagga tgagccgccc gttcgagcgg taccccgcac
     7741 ctgttagaat ccgccggtcg gcgctcagga ggcatgctcg tgaagacgaa gacgatctgg
     7801 ttcgcgttgt tcctcctcta cttcggcctg ctggcgctcc ccgcgcaggc gtacctcgac
     7861 ccggggaccg ggagcgtcat cttccaggtc atcgtggggg gtctgctcgg cgcggcgttc
     7921 gcgatcaaga cgttctggcg gcggatcgtc ggcttcttca gtcggcggaa gcccgagtcc
     7981 gagcgggttg actgacccag acgtccttcc cagctccttc cgggacccca gcggattcgt
     8041 cttccggcgc gaggggatcc tctaccgcca ggtcaaccgc tctttccagg agagcttcga
     8101 cgcgctgctg tcgtccggcc tgtacgacgc gctgacgggc gatgggctgc tgatcccgca
     8161 cgaggaagcg ggggagccgg caaccgacga ggcgtaccgg gtcctgcggc ccgagccgat
     8221 cccgttcgtc tcgttcccct acgagtggag cttctcccag ctgaaggacg cggcgctcgc
     8281 gaccctgcgg atccagtcgc tcgccctcga tcacgggatg acgctgcgcg acgcgagcgc
     8341 ctacaacatc cagttccggg acggacgccc gatcttcatc gacaccctct cgttcgagcc
     8401 atggacggag ggacagccgt gggtcgccta ccggcagttc tgccagcact tcctcgcgcc
     8461 gctcgcgctg gctgccttcg ccgacatccg gctcctgcag ctcctccgca cgaacatcga
     8521 cggcgttccc ctcgacctcg ccgcgcgact gctgccgggc gcggcccggc gtcgcttggg
     8581 catccggctt cacgtcgtcg cccacgcgaa gagccagcgg cgccaccagg gcgatgccga
     8641 agcgccatcg cgggggcgga gcttctcgat gcgcgcgttc cgggggctgc tcgacaacct
     8701 cacctcgacg gttcgcgcgc tttcttggag ccccggtcgc accacctggt cgagctacta
     8761 cgaggaggcg gatcactaca gcgacgaagc gttcgagcgg aagcggtcgc tcgtggatcg
     8821 gttcatcggc gagacctccc ccgcgagcgt gtgggatctc ggcggcaaca tcggcgtctt
     8881 cgcgaggatc gcgtcggagc gcgggatccc gacggtctgt ttcgacctcg acgaggctgc
     8941 ggtggaagcg aactaccgac gggtgcgcga gcaggccgag cgacagctcc tcccgctcgt
     9001 cttggatctc acgaacccga gcccggcgct cggatgggcc ggcgccgagc gtccgtcgct
     9061 cgcccagcgc ggtcccgtcg acctcgccct ggcgctcgcg ctcgttcacc acctcgcgat
     9121 cggcaacaac gtcccgctgc agcggatcgc ggcgtacttc gccgacctct gcgagggcct
     9181 ggtgatcgag ttcgtcccga aggaggacga gaaggtccga accctgctgg cgacccggga
     9241 ggacgtcttc cccgactaca cggaagaggg gttcgagcgc gccttcgagg agcggttcgc
     9301 gatcgggtcg aaggagccga tcgaaggctc cgcccgcacc ctctacctga tgaagcggcg
     9361 ctgatgcgct ggccacggcc gctcgtcctc catccgctcc tgttcgccgc gctccccgtc
     9421 gtgtacctgt acgccgcgaa cctcgcggag acgaacctga gcgatgtcct cgcgccgctg
     9481 gcagtgatcc tggtgggcgc cgtgatcctg ctggcggcgg cggccttcct cctccggcgc
     9541 gtccacgccg ccggcgtcgc ggtcagcggc ctgatcctcc tgttcttctc ctacgggcat
     9601 gtgcaccgtg gcctcgccgg cacaccggtc ggacggcatc gtgtcctgct cgtcctctgg
     9661 gcggcgctcg ccctcgccct cgtcgccgcc gtacggggac gtcgctggcc cgtccgtgcc
     9721 accggggcgc tcaacttcgt cgcggctggg ctggtcctcg tgaacgttgc gacgatcgcc
     9781 ctgtacaacc tgcgaagcag gccggccgag gtcggggatc cagggatcgc tgccgcgccg
     9841 aggacggcgc cggaccggga tatctactac ctcgtcttcg accgatacgg cggtgagcgg
     9901 gcgctccggg gaagcttccg gttcgacaac tccaccttcg tcgaaggact ggagaagcgt
     9961 gggttctacg tggccgaccg cagcagcggg aactacccgc ggacgctcca ctcgctggcg
    10021 tcatcgctga acatggacca cctgctcgag ctcgcgcgac gcgtgggcga agactccggc
    10081 gacggcggcc cgctgcgcga gatcctcgcc gatcaccggg tcgggcgttt cctcaaggag
    10141 gccgggtatc gctacatcca ggtcggctcc tggtgggctc cgacggcgac gagcccgctc
    10201 gccgaccgaa cgatgcgggc gggactcctg tccgagttct cggcggttct cctctcgacg
    10261 acggcggccg ctcccgtgct cgagatggtg tcgggtgaga tggagccgcg ccgccgcgag
    10321 tacaaccgca cgctccagca gttcgagaac ctgacgaagg cgtcacgcct gccggggccg
    10381 aagttcgtgt tcgcccactt cctcgttccg cacacgccgt acgtcttcga ccggaacggc
    10441 gccttcctgc cgcaggagat ccacgacgcc cggagcgagg gcgagaacta cgtggagcag
    10501 cttcgctacg tgaaccgccg gatcgagcag ctcctcgacg agatcctggc ggtccccgaa
    10561 aaggagcgcc cgatcgtcct cctccaatcc gacgaggggc cgggcgagcg gccggagagc
    10621 tgggaaggcg tgccgcagcg cgtgctcgaa gagaagttct tcatcctcaa cgcgtaccac
    10681 ctccccggcg tggaggaccc gcccctgtac ccgacgatca cgcccgtgaa ctccttccgc
    10741 ctcgtgctca acctgtactt cggtgcggag ctcccgctgc ttcccgaccg tgtctacgtc
    10801 ttccaggaca tcgatcacct ctaccgtttc accgacgtca ccgagcggct cttcggggac
    10861 gacccgtagc tcccggggtc gccgtcagcg agcgacgagt gactccgcaa ggacgccgag
    10921 cgcttcgtcg aggagctcct cgtcgatcgt cagcgggggg aggaggcgca cgacgttgtc
    10981 gtacgtcccg gccttgagga cgacgacgcc gttgcggtag cactcctcga tcacgcgcgc
    11041 ggcggcctgc ttcgcgggct ccttcgtgtc ggggtcgtcg acgagctcga ccgcggccat
    11101 cgcgccgagg ccgcggacgt cgccgatcag atcggtctcg gtcgcggccg tttccaggcg
    11161 cccgcgcatg cgctcgccga tccgctcggc ccgcgcgcac aggtcgtcgc gctcgatcag
    11221 gtcgatgacc gccagcgcgg ccgcgcaggc gatcgggttg ccgccgaagg tcccgccgag
    11281 cccaccgacg tgaacgccct ccatcagctc gcggcggccg gtcacggcac cgatcggcag
    11341 tccgccgccg aggcccttgg cgctcacgac gaggtcgggg acgagccccg agtgctcgat
    11401 cccgaaccac cgaccggtcc gagcgaagcc cgtctgcacc tcgtcggcga tgaagacgat
    11461 gccctcctcg cggcagaacg cgtgcagcgc ctcgaggaag ccttcgcccg ggacgacgaa
    11521 cccgccctcc ccctggaccg gctcgaccac gaggcaggcg atgttcccgg gaccgagctc
    11581 gcgcatccgg tcgatcgcgc gctccgcgca cgcgggaccg cactcctgcg gcgacgcacc
    11641 cgcggggcag cggtacggat aggcgtacgg aaggcggtgc acgtccgggg cgaacgggcc
    11701 gaacccgtgc ttgtacggca tgtccttcgc cgtcagcgtc atcgcgagga gcgtgcgtcc
    11761 gtggaacgca tggtcgaagg cgacgacgcc ggcccgaccg gttgcgtggc gagcgatctt
    11821 gaccgcgttc tcgacggcct ccgcgccgga gttcacgagc atcgtctgct tggcgtgctc
    11881 gccgggcgtg agctcgttca gacgctcggc cagctcgacg tagggctcgt gcatcgtcac
    11941 gtggaagcac gtgtgcgtga acgcttccgc ctgtcgtcgc acggcctcca cgacctccgg
    12001 tgcggcgttc cccacgttca gcacggcgag cccggccccg aggtcgatca ggcggttgcc
    12061 gtccacatcc tcgaggaccg cgccgtcggc gtcctggacg aagaccggca ccgtgttgaa
    12121 gacggccggc ggaacggcga gggcgcgccg gtcgagcagc tccttcgacc gcggtccggg
    12181 gatctcggtc accagtcgcc gctgctgggg gatgctcatg cgagcgagtg tggcgcgccc
    12241 gcccggcccg cgcgagggcg ccgcggtgaa cgcggcggac cacgtttgta cgccccgtac
    12301 acccccctcg ctgttgggcg gagcggcccg gcacgtcccg gcgtgccgga acgtacaatc
    12361 gacgggtggc cggggtccgt gtcgccgaca tcgtcgagct cgactccctc gggctgaacc
    12421 tcgtctccgg gcggcgcggc gcgcggcacc cgatccgctg ggtgcacgtg agcgagctgg
    12481 aggaccccac cccgtggctc aagggcggcg agctcctgct cacgaccggc atggggctcg
    12541 ggaagtcggc atcccggcag cgcgcctaca tcgagcgcct cgcctcggcg ggcctggccg
    12601 ggctcgggtt cggcatcggg ttcacgtacc gccgcgtccc gaaggcgatc gtcgacgcgt
    12661 cggagcgcct gtcgttcccc gtcttcgagg tcccctatcc ggtcccgttc atcgcgatca
    12721 ccgaggcggt gtactcgcgg ctctcggccg agcaggtgga cgtcctgcga cgggcgctcg
    12781 aggcgcaggg cgcgctgacg cgcgcggtgc ttgcgggaga cggggtcgcc ggcgtggtcg
    12841 cggcgctgcg cggcgccacc ggcggctggg cgctcgccct cgacgtgcgc ggcggggtcg
    12901 tggcggcgtc tccgtccgcg gtcgcgtccc gagcgcccag gatctgggag gaggtgcgcg
    12961 cggcgcgccc cagcggtccc gggttcgcgc tcagcgtcaa cgaccgcgac gaccacgtct
    13021 cgatccatcc gatcggcgtg cacggccacg tcgagggcta cctggcgacc ggtgccgccc
    13081 atccgccggg ccagctcgag cggatcgtcg gcggccacgc gctctcgctg ctcgcgctcg
    13141 agctcgacaa gacgcgcgcg ctcgcggacg cggagcggcg gctccgcggt gacctgttcg
    13201 acgccctcgc gcggggcgag ctcagtgaga gcgaggcccg gcgtggcctc cggcggttcg
    13261 gcttcgaccg tgccgacgcg gtggcggtcg tggcgatcga ggcggaagct ccgatgcagg
    13321 atctcgcgtg ggcggcggag gacctgctcg cgctcgacgg tgttccgttc ctcacggccc
    13381 cccgcgatcg cacgctcttc gtgctgctcc ggccgaacgg ccgcggaacc ctcgaggatc
    13441 tccaccggcg cctgtcggag cggctgccgg aaggggtgct cgcggcggcg gggagcgccg
    13501 tcgaacctgc cgaggcggcg aggagcctgc gcgaggctcg ctacgccctc gaggtctgcc
    13561 gcgcgcaggg gcgctcgttc gccgatgtgc acgcgctcgg gacctaccgg ctgctcctga
    13621 cgcttcagga gccggacgcg ctgcgggcct tcgccgacgc cgtgctgacc cccctcgacg
    13681 agtacgaccg gagcggggag ggcgagctgg tcccgtcgtt gcaggcgttc ctcgagcaca
    13741 acgcgcgctg ggagtcggcg gccgcggcgc tgttcgtcca tcgccacacg ctccgctacc
    13801 ggatgcgcaa ggtcgaggag ctcaccggtc gcgacctctc gtcggcgcac gaccggatgg
    13861 agttctggct cgcgttgcgc gcccgcgaga tggtcgcgcg cgagaccgag gaggtctgat
    13921 gttgtccctt ccgtacagcc gagcggcggg aggatgaaca gggcggccga gggcccctga
    13981 gcgctcggtc gatagcctgt ccgcagacgc gcgaggagga acgatgacga cgaagcagat
    14041 gttcatcggt ggggactggg tcgacgggag cggtggctcg gtcgaggtga tcaacccggc
    14101 cacgggggag cgactcgcgg agacgccgaa cgcgaccgtg gaggacgtgg atcgcgccgt
    14161 gaccgcggcg cagcgcgcgt tcgacgacgt gtggttcgac tccacccccg ctgagcggca
    14221 ggcgatgctg ctgaagtggg ccgaccggat cgaggagcac gccgaggagc tcggccggat
    14281 cgagtcggag aacgtcggca agccgatgtc gtgggtcgtc tccgacgaga tcctggtggt
    14341 ggccgagcag atccgcttct tcgccggcgc ggcgcgggtg atgggcggcc tctccgccgg
    14401 tgagtacttc aaggacgact ccacgatgta cacgagctgg ctccgccggg agccgatcgg
    14461 cgttgtcggc ctgatcgcgc cgtggaacta cccgctctac atggcgatgt ggaagttcgc
    14521 acccgccctg gccgcgggga acaccgtcgt gatcaagccc tcggagtgga ccccgctgac
    14581 gctcctccgc gcgacggagc tggcggcgga catcttcccg cccggggtca tcaacgtcgt
    14641 gacgggtgaa ggtcagccga ccggggccgc gatcgtcgag cacccgggcg tcgggatggt
    14701 ctcgctcacg ggagaggtga cgaccgggaa ggaggtcgcg cgcgcggccg cctcgacgct
    14761 caagcgcgtc cacctcgagc tcggcggcaa ggcgcccgtg gtcgtgttcg acgacgccga
    14821 ccccgcggcg gtcgcggagg ggatcaaggt cgccgggtac tggaactccg gccaggactg
    14881 cactgccgcc tcgcgcgtc
//
LOCUS       DATLGG010000184         4225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_71059, whole genome shotgun sequence.
ACCESSION   DATLGG010000184 DATLGG010000000
VERSION     DATLGG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71059"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1535)
                     /locus_tag="VM638_05260"
     CDS             complement(90..1535)
                     /locus_tag="VM638_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11866.1"
                     /translation="MRIRLTARLAVLALLGAVPIGAVAQERVCPKPADFAPPPLTFSP
                     PSYIDTSRAGGEPVSVIGHDGSINVSAHAGTTHVYKNPSAAPGAGDFLVGYANQTLNW
                     RSTDGGQTWKYVGTLGLPAGPHSLTSTGFSDPDFAIDQAGKIYNVEIDLANNAVFSSN
                     DDGQSYAIAHPQVAPGDRPWLAALEKDEVFLYINSLPKSFWRSTDGGITWSLISTSPP
                     FNGDFHPDPRNPNDGLIGPVGTYGAAISADDGRTWELHYGANTGSNTGFNGIATVGID
                     RAGHIYQAAAGSGEVSFSAFNRDTKTWGPRVDIPHPAGGDTLWPWIVAGDDGRVAMTW
                     YQTLPGKPDEYYVYASYTLNARGTVAQCSDGSTVFIPPQFSTANASGRMIHKGQICLQ
                     GTICNADLGEGGDRRLGDFFTINFTHAGEIFIASGDTMLRSPTGGPKPVANPIFIKQS
                     GGAPLLAKPDRVRPSRCLLDLPVCPPPRAPR"
     gene            complement(1619..1903)
                     /locus_tag="VM638_05265"
     CDS             complement(1619..1903)
                     /locus_tag="VM638_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HVM11867.1"
                     /translation="MLHMVVNTHNPESCGFRGDEENRILEEALDAFKATGGEVSLKGW
                     WVHPPGHEIFMLVEAPNAHAVQGALLEAGLVGRTHSRILPVLSVEEVFGD"
     gene            complement(1932..3344)
                     /locus_tag="VM638_05270"
     CDS             complement(1932..3344)
                     /locus_tag="VM638_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HVM11868.1"
                     /translation="MLASGDLGGAWTTSPEPILAGDVLSQVDRVGTADILVGIPAFNS
                     AETIGYVITAVEAGLRKHFPDQRAVICVSDGGSEDGTMEVAVRAGVGDDHEQLLVDPT
                     TPVPKKIVFQYRGPSGKGSAFRSIFDVAVAVDAKACAVVDSDLRSITPYWLDRLLGPV
                     VKSGYGFVAPVYSRHKFDGTITNSFAYPLTTALYGERIRQPIGGEFGFSGALARTYAG
                     AEDVWESDVARFGIDIWMTTVAVVEGFRTCQGILGAKIHDPKDPGKDLGPMFRQVVGS
                     LFALAGRYEDRWYDLSEVNTPPRFGFNAAYSAEPIQVSVPRLTWKFVEGYVRHHTLWR
                     EVLSEDSMAGVEKAVADAAESTRGLVLEPETWTTIVYDYLVAYNAGEIDAGPLLDSLI
                     PLYFARTGTFVQEVSADSYEEAEMKVEQAVDLAIELKPYLRKRWDERQVSPRPLSDQT
                     VPSGPPPDERLEGAIRAEIP"
     gene            3385..>4225
                     /locus_tag="VM638_05275"
     CDS             3385..>4225
                     /locus_tag="VM638_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVM11869.1"
                     /translation="MRIGQVSPPWLAVPPKGYGGIEWVVALLADGLAERGHDVTLFAT
                     GDSTTAARLEYVFEEAPGPTAINSIWHDVRQSLLAYRDPARFDLIHEHGLWSGLVAAA
                     LSPVPVVHTLHGQFSEEMRAIYEPLADRLWFVAISERQRSHMPDLRYAGVVYNGIDVG
                     RYPLREEKDDFLLFLGRANPDKGPLRAVEAARASGLPLVMAVKAASEIEVRHWEDEVL
                     PRVPEGTTLLGEITIDEKLDLLSRARAVLFPIDWEEPFGLVMTESMACGTPVLATPRG
                     SVPE"
BASE COUNT          608 a         1429 c         1505 g          683 t
ORIGIN      
        1 ctgccctccc ccccgcgctc cgaggtagcc ctcccggggg ccggtttgtg caccctttgg
       61 gcgggtgcac aaaccggacc ccgggagggc tacctcggag cgcggggggg agggcagacc
      121 ggcaggtcga ggaggcacct cgagggccgc acccggtccg gcttcgccag caacggggcc
      181 ccgccgctct gcttgatgaa gatcgggttc gcgaccggct tcggcccgcc ggtggggctg
      241 cggagcatcg tgtcgccgct cgcgatgaag atctcgccgg cgtgcgtgaa gttgatcgtg
      301 aagaagtccc cgagccggcg gtcgcctccc tcgccgaggt ccgcgttgca gatcgtgccc
      361 tggaggcaga tctgtccctt gtggatcatg cgccctgacg cgttcgccgt cgagaactgc
      421 ggcgggatga agacggtcga gccgtcggag cactgggcca cggtgccgcg cgcgttgagc
      481 gtgtacgagg catagacgta gtactcgtcc ggtttcccgg ggagggtctg gtaccaggtc
      541 atcgcgacgc gtccgtcgtc accggcgacg atccacggcc acagggtgtc gccaccggcg
      601 gggtggggga tgtcgacgcg cggcccccag gtcttggtgt cccggttgaa cgcggagaac
      661 gagacctctc cgctgccggc ggccgcctgg tagatgtgtc cggcgcgatc gattccgacg
      721 gtcgcgatcc cattgaagcc cgtgttcgat ccggtgttgg cgccgtagtg gagctcccag
      781 gtccggccgt cgtccgcgga gatcgcggcg ccgtaggtcc caaccggccc gatcaacccg
      841 tcgttcgggt tgcgagggtc ggggtggaag tcgccgttga acggcggcga ggtcgagatc
      901 agcgaccagg tgatcccgcc gtcggtcgat cgccagaacg atttcggcag gctgttgatg
      961 tagaggaaca cctcgtcctt ctcgagagcg gcgagccacg ggcggtcgcc cggcgccacc
     1021 tgcgggtgcg cgatcgcgta cgactgcccg tcgtcgttgc tcgagaagac ggcgttgttc
     1081 gccaggtcga tctcgacgtt gtagatcttg ccggcctggt cgatcgcgaa gtcggggtcg
     1141 gagaacccgg tcgaggtcag gctgtggggc ccggcgggga gcccgagggt cccgacgtac
     1201 ttccaggtct ggccaccgtc ggtcgagcgc cagttcagcg tctggttcgc gtagccgacg
     1261 aggaagtcgc cggcgccggg cgccgccgag gggttcttgt agacgtgcgt ggtgccggcg
     1321 tgcgccgaga cgttgatcga cccgtcgtgg ccgatcacgc tcacgggttc gccgcccgcc
     1381 cgggaggtgt cgatgtagct cgggggcgag aacgtgagcg gcggcggcgc gaagtcggcg
     1441 ggcttcgggc agacgcgctc ctgcgccacg gcgccgatcg gcaccgctcc gagcagggcg
     1501 agcacggcga ggcgagcggt cagacggatg cgcatgggtc cccccgttcc ggttacgtca
     1561 cggagggttc gccatcagcc acgagcgttc cttcgtgcag atgcacaaac cggcaggctc
     1621 agtcgccgaa gacctcctcg acgctgagga ccgggaggat gcggctgtgg gtgcggccga
     1681 cgagtccggc ctcgaggagc gcgccctgga ccgcgtgcgc gttcggggcc tcgacgagca
     1741 tgaagatctc gtggcccggc gggtgcaccc accagccctt cagcgacacc tcaccgcccg
     1801 tggccttgaa cgcgtccagg gcttcctcga ggatccggtt ctcctcgtcg ccgcggaacc
     1861 cgcagctctc ggggttgtgc gtgttcacca ccatgtgcag catgcgtatc cctccctgat
     1921 gatcgttccg cctatgggat ctcggccctg atcgcgcctt cgagccgctc gtccggcggc
     1981 ggcccggacg gaacggtctg gtccgagagg ggccgcgggc tgacctgccg ctcgtcccag
     2041 cgcttccgca ggtacggctt cagctcgatc gcaagatcga ccgcctgctc gaccttcatc
     2101 tccgcctcct cgtagctgtc ggcggatacc tcctgcacga acgtgccggt ccgggcgaaa
     2161 tacagcggga tcagcgagtc gaggagcggc cccgcgtcga tctcaccggc gttgtaggcg
     2221 accaggtagt cgtagacgat cgtcgtccag gtctccggct ccagcacgag gccgcgcgtg
     2281 ctctccgcgg cgtcggccac cgccttctcg accccagcca tcgagtcctc ggacagcacc
     2341 tcccgccaca gggtgtggtg ccggacgtag ccctcgacga acttccacgt gagccgcggc
     2401 accgagacct ggatcggctc cgcggagtac gcggcgttga acccgaaccg tggcggggtg
     2461 ttcacctcgc tcaggtcgta ccagcgatcc tcgtagcggc cggccagggc gaagaggctg
     2521 ccgacgacct gccggaacat cgggccgagg tccttcccgg gatccttcgg gtcgtggatc
     2581 ttcgccccga ggatgccctg gcaggtgcgg aagccctcca ccacggcgac ggtcgtcatc
     2641 cagatgtcga tcccgaaccg agcgacgtcg gactcccaga cgtcctcggc gcccgcgtag
     2701 gtccgggcga gggcgccgga gaacccgaac tcgcccccga tcggctggcg gatccgctcc
     2761 ccgtagagcg ccgtcgtcag gggataggcg aaggaattcg tgatcgtccc gtcgaacttg
     2821 tgccgcgagt agacgggcgc gacgaacccg tagccgctct tcacgaccgg cccgaggagg
     2881 cggtcgagcc agtagggggt gatgctccgg aggtcggagt cgacgacggc gcaggccttc
     2941 gcgtcgaccg ccacagcgac gtcgaagatc gagcggaagg cgctgccctt cccggacggt
     3001 ccccggtatt ggaagacgat cttcttcgga acgggggtcg tcgggtcgac gaggagctgc
     3061 tcgtggtcgt cgccgacgcc cgcacggacg gcgacctcca tcgtcccgtc ctccgacccg
     3121 ccgtcggaga cgcagatcac ggcgcgctgg tcggggaagt gcttccggag gcccgcctcg
     3181 acggcggtga tcacgtagcc gatcgtctca gcgctgttga acgccgggat gccgacgagg
     3241 atgtcggcgg tccccacgcg gtcgacctgc gagagaacgt caccagcgag gatcggctcc
     3301 ggggacgtcg tccatgcacc tccaaggtcg cctgatgcca gcatacccgt tcgtcctccg
     3361 gggcgagcgg atatcgtgac cgcgatgcgg atcgggcagg tctcaccgcc gtggctcgcg
     3421 gttcccccga aggggtacgg gggcatcgag tgggtcgtcg ccctgctggc ggacgggctg
     3481 gcggagcgcg gccacgacgt caccctgttc gcgaccggcg actcgacgac cgcggcgcgc
     3541 ctcgagtacg tcttcgagga ggcaccgggc ccgacggcca tcaactcgat ctggcacgac
     3601 gtgcgccagt ctctgctggc gtaccgagac ccggcccggt tcgacctgat ccacgagcac
     3661 ggcctttggt cgggcctcgt cgcggctgcc ctttcgccgg tcccggtcgt ccacacgctc
     3721 catgggcagt tctcggagga gatgcgcgcg atctacgagc ccctcgccga ccggctctgg
     3781 ttcgtcgcga tcagcgaacg gcagcgctcg cacatgccgg acctgcgcta cgccggcgtc
     3841 gtctacaacg ggatcgacgt cgggcggtat cccctccgcg aggagaagga cgacttcctg
     3901 ctgttcctcg gccgcgcgaa cccagacaag gggccccttc gggcggtgga ggcggcgcgg
     3961 gcgtcgggtc tccccctcgt catggccgtg aaggcggcct cggagatcga ggtgcggcac
     4021 tgggaggacg aggtcctgcc gcgcgttccg gagggaacga ccttgctcgg agagatcact
     4081 atcgacgaga agctcgatct cctgtcgcgc gcacgcgccg tgctgttccc gatcgactgg
     4141 gaggagccgt tcggactcgt gatgaccgag tcgatggcct gcggaacccc ggtgctcgcg
     4201 accccccgcg gctccgttcc ggagg
//
LOCUS       DATLGG010000185         3805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_58812, whole genome shotgun sequence.
ACCESSION   DATLGG010000185 DATLGG010000000
VERSION     DATLGG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58812"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VM638_05280"
     CDS             complement(<1..1434)
                     /locus_tag="VM638_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HVM11870.1"
                     /translation="MRTALVAAALAVGLLAAALPAGADGNPNTSAKLREAVTLQGVRR
                     HQQAWQQIADANGGNRFAGLPGHGKSAEYVIEQLEAAGYQPVVHEFDYEAFFERTTSQ
                     LAQVSPNAVTYVNGNDFRIMSYSGSGSVTAPVFHLGAGVLGCNPADFAALPAGSIALV
                     SRGACTFRIKTDNAAAAGASAILIYNNTVGVINGTLGATGLSAIPSLGITQSLGQSLA
                     SQLTSGPVIMRVLTDTAEETLPTYNVLAEKTGIQNPQNVVMAGAHLDSVTAGPGINDN
                     GSGSAAILETAIQMAKVKPFNTIRFAWWSAEESGLIGAREYVTSLTQAERDKIALYLN
                     FDMVGSPNFVRFVYDGDNSAELPSGVAGPAGSETIEQLFVDYFASQGLESAPTPFNGR
                     SDYGPFIDVGIPAGGLFTGAEGIKTAAQADIYGGLAGVAFDPCYHAACDDYENNSDLG
                     LDQMSDAIAHSIITYAQSTTAINQVRGK"
     gene            complement(1581..1886)
                     /locus_tag="VM638_05285"
     CDS             complement(1581..1886)
                     /locus_tag="VM638_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11871.1"
                     /translation="MRTDRAWLPALGWALAGLIFSVGLTLAAYALVGGGAAQPALPVL
                     GDLPTPTASPSGSPSATPTTGVDVSGPCDEPEHRNDPRCLDGGSRSGSNRGPGSGDD"
     gene            complement(1883..3199)
                     /locus_tag="VM638_05290"
     CDS             complement(1883..3199)
                     /locus_tag="VM638_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVM11872.1"
                     /translation="MLTVIVAFGIPLALSFERRVVAEAQGRSLAQAQAIASAVGDPRE
                     DEDEIPRVIADAARVIGGRVTVVDAAGAVVADSSGTPPGDAATEVLRALGSRAPVSEV
                     RRGEGPEVVGTSVPIVEEGEVVGAVGIEVPLDRVRAGLPRTYLALGVVGSAALLAGLA
                     IAFALAGSFARPLRRLAASAARLGRGDLGVRTGEVRAPREIREVATSFDEMAERLQAT
                     VRAQRRFAGNASHQLRTPLTALKLRIEAALAGAPEDLRPELEAADHEVDRLTAIVERL
                     LVLSRRVERTGPWESSLGDLAAAAVDRWRARLAQARRPINATGDDARAAANPADVEQI
                     LDNLIENALVHGGGAVTIETRAAGARAMLAVEDRGRGIRDEEAARVLERFYRGSEAPA
                     GGSGLGLAIVRDLAERWEGEVRVGPGPGGGARVEIELPAPPPTRAR"
     gene            complement(3251..>3805)
                     /locus_tag="VM638_05295"
     CDS             complement(3251..>3805)
                     /locus_tag="VM638_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM11873.1"
                     /translation="DLVLLDLMLPDGNGRDLFAEMRSTSDVPVIIVTARGEEVDRVVG
                     LELGADDYVVKPFSARELVARIRAVLRRAAPGGGGGTIRIGRVELDPEARLVTKDGEP
                     VALAAREFDLLRLLMENAGRVLRREEIMDEVWDPHWFGPTKTLDVHISWLRKKLEDDA
                     AEPRYITTVRRVGFRFAAAAEVEG"
BASE COUNT          520 a         1428 c         1313 g          544 t
ORIGIN      
        1 cttgccgcgg acctggttga tggccgtcgt gctctgggcg tacgtgatga tcgaatgcgc
       61 gatcgcgtcg ctcatctggt cgaggcccag gtcgctgttg ttctcgtagt catcgcaggc
      121 ggcgtggtag caggggtcga aggcgacccc cgcaaggccc ccgtagatgt ccgcctgcgc
      181 ggcagtcttg atcccctcgg cgcccgtgaa cagaccgccc gctgggatcc ccacgtcgat
      241 gaacggcccg tagtccgacc gtccgttgaa cggcgtgggc gcgctctcga gcccctggct
      301 cgcgaagtag tcgacgaaga gctgctcgat cgtctccgac ccggcagggc ccgccacgcc
      361 gctcggcagc tcggccgagt tgtcgccgtc gtacacgaac cggacgaagt tcggggaccc
      421 caccatgtcg aagttgaggt acagcgcgat cttgtcccgc tcggcctgcg tcagcgatgt
      481 cacgtattca cgagcgccga tgaggcccga ctcctccgcg ctccaccagg cgaaccggat
      541 cgtgttgaac ggcttcacct tcgccatctg gatcgccgtc tcgaggatgg ccgccgagcc
      601 cgagccgttg tcgttgatgc ccggtcccgc ggtgaccgaa tccaggtgcg cgcccgccat
      661 caccacgttc tgcgggttct ggatgccggt cttctcggcg aggacgttgt aggtcggcag
      721 cgtctcttca gcggtatccg tcaggacccg catgatgacc ggccccgagg ttagctgcga
      781 ggcgagcgac tgccccaggc tctgcgtgat cccgagcgag gggatcgccg agaggccggt
      841 ggctcccagc gtcccgttga tgacgcccac ggtgttgttg tagatcagga tcgccgatgc
      901 cccggctgcg gcggcgttgt ccgtcttgat ccggaaggtg cacgcgcccc tgctgacgag
      961 cgcgatcgac cccgccggga gggcggcgaa gtccgccggg ttgcagccga ggacgcccgc
     1021 acccaggtgg aagaccggcg ccgtcacgct cccgctaccg gagtagctca tgatccggaa
     1081 gtcgttgccg ttcacgtagg tgacggcgtt cggggagacc tgcgccagct gtgacgtcgt
     1141 cctctcgaag aaggcctcgt agtcgaactc gtgcacgacc ggctggtacc cggccgcctc
     1201 gagctgctcg atcacgtact ccgcgctctt cccgtgcccg ggaaggccgg cgaagcggtt
     1261 ccctccgttg gcgtccgcga tctgctgcca cgcctgctgg tggcggcgaa ccccctgcaa
     1321 cgtgactgcc tcccgcagct tcgcggaggt gttcgggttc ccatccgctc ccgccggcag
     1381 cgcagccgcc aggagcccca ccgccagcgc agcggcgacc agcgccgtgc gcatccacgt
     1441 ccttcgatcg tccatccagc acctccctgc tcacggagtg ccggtcccat cgatcggccc
     1501 cctccctgga agcagagggt tcgacgcgcg ctgcccgtcc acctttttct ttccttgcgg
     1561 atcggagcgt ccccgtgacc tcagtcgtcg ccggaacccg gtccccggtt ggagccggaa
     1621 cggctgcctc cgtcgaggca ccgagggtcg ttccggtgct caggctcgtc gcagggcccg
     1681 gaaacgtcga cgccggtggt gggggtcgcg gaggggcttc cggacggcga ggccgtgggt
     1741 gtcggcagat cgccgaggac cggcagggcc ggctgggcgg cgccgccgcc gaccagtgcg
     1801 tatgccgcga gcgtgaggcc gaccgagaag atcagccccg ccagggccca cccgagggcc
     1861 ggcagccacg cccggtcggt cctcatcggg ccctcgtggg cgggggcgcc ggaagctcga
     1921 tctcgacgcg cgccccgccg cccgggcccg ggcccacgcg cacctcgccc tcccagcgct
     1981 ccgccagatc gcggacgatc gcgaggccga ggccggaccc gccggcgggg gcttcggacc
     2041 cccggtagaa ccgctccagg acccgcgcgg cctcctcgtc ccgtatccct cggccgcgat
     2101 cctccacggc cagcatcgcc cgggcgcccg ccgcccgcgt ttcgatcgtc acggcaccac
     2161 cgccgtgaac gagcgcgttc tcgatcaggt tgtcgaggat ctgctccacg tcggccgggt
     2221 tggcagcggc ccgagcgtcg tcgccggtgg cgttgatcgg cctcctcgct tgggccaggc
     2281 gggcgcgcca gcggtcgacc gcggcggcag cgaggtctcc gagcgaggac tcccacggcc
     2341 ccgtccgctc cacgcggcgc gacaggacca ggagccgctc cacgatggcc gtgagccgat
     2401 ccacctcgtg gtcggcggcc tcgagctcgg gacggagatc ctccggcgcg ccggcgagcg
     2461 ccgcctcgat ccggagcttc aacgccgtga gcggggtccg cagctggtgc gaagcgttcc
     2521 ccgcgaaccg gcgctgggcc cgcaccgtgg cctggagccg ctccgccatc tcgtcgaagg
     2581 acgtggcgac ctcccggatc tcgcgggggg cccggacctc gccggtgcgg acgccgaggt
     2641 ctccccggcc gagccgggcc gcggatgccg cgagccgccg gaggggccgg gcgaaggacc
     2701 cggccagcgc gaacgcgatc gcgagaccgg ccaggagagc agccgaaccg accaccccga
     2761 gggcgagata ggtgcgagga agcccggctc gcacgcggtc gagcggcacc tcgatcccga
     2821 ccgcgccgac cacctctccc tcctccacga tcggcacgga cgtgcccacc acctcggggc
     2881 cctcccccct gcggacctcg ctcaccggcg cgcgggagcc gagggcgcgg aggacctccg
     2941 tcgcggcgtc ccccggaggc gtccccgacg agtccgcgac gacggcaccg gcggcgtcga
     3001 cgaccgtgac ccggccgccg atcacgcgcg ccgcgtcggc gatcacacgg gggatctcgt
     3061 cctcgtcctc ccgcgggtca ccgaccgccg acgcgatggc ctgggcctgg gccagtgagc
     3121 gtccctgcgc ctcggccacg accctccgct cgaagctcag cgccaggggg atgccgaagg
     3181 cgacgatcac cgtcagcacg atgtaggcga aggcgaggac gaggcgggcg cggacccccg
     3241 tgaccgcctc ctacccctcg acctcggccg cggccgcgaa gcggaacccc acccggcgaa
     3301 cggtcgtgat gtagcggggt tccgccgcat cgtcctcgag cttcttccgc agccacgaga
     3361 tgtgcacgtc gagcgtcttc gtcgggccga accagtgcgg gtcccagacc tcgtccatga
     3421 tctcctcgcg ccgcaggacc cgcccggcgt tctccatcag gaggcgcagg aggtcgaact
     3481 ctcgagcggc gagcgcgacc ggctctccgt ccttcgtcac gagccgggcc tccggatcga
     3541 gctccaccct cccgatccgg atcgtcccgc cgccgccgcc gggcgcggct cgccggagca
     3601 ccgcacggat cctcgcgacg agctcacgcg ccgagaacgg cttcacgacg tagtcgtcgg
     3661 cgccgagctc cagcccgacg acgcggtcga cctcctcccc gcgggcggtc acgatgatga
     3721 ccgggacgtc ggaggtcgag cgcatctcgg cgaagaggtc gcgcccgttg ccgtcgggga
     3781 gcatgaggtc gaggagcacg aggtc
//
LOCUS       DATLGG010000186         5894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_58818, whole genome shotgun sequence.
ACCESSION   DATLGG010000186 DATLGG010000000
VERSION     DATLGG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58818"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VM638_05300"
     CDS             complement(<1..283)
                     /locus_tag="VM638_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11874.1"
                     /translation="MTNETAGAAGAVTNSVAATLGQSKLVKAGRADVARIRERTRRRR
                     LLKLAIVLGIVDAWLWKRLAEGNPVGLPSMPEDAMLWLPGFLLIGILAIV"
     gene            411..800
                     /locus_tag="VM638_05305"
     CDS             411..800
                     /locus_tag="VM638_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11875.1"
                     /translation="MPRKPQFEREDARLVGGLLRDLRRAAGFRAVKDAAAAEGSPAAR
                     QTIYAYERGGLCPSLPQFLELVEFYALKAPAADPAIRHMAIAAIHRALALPMYHVRSA
                     LELIERLQPEPSRARRRGGAASEDEEG"
     gene            805..1206
                     /gene="mce"
                     /locus_tag="VM638_05310"
     CDS             805..1206
                     /gene="mce"
                     /locus_tag="VM638_05310"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HVM11876.1"
                     /translation="MIEDIDHVGIAVGDLETAVERYRAAYGIEPAHRETVEEQGVEEV
                     LFAVGTSYIQLLRPLGPETPVGRFLEKRGEGIHHVAYRVRSVREGLDRLRAEGHRLID
                     EEPRRGSRGTTIAFVHPKAFDGVLIELVEEG"
     gene            complement(1203..2378)
                     /gene="ccrA"
                     /locus_tag="VM638_05315"
     CDS             complement(1203..2378)
                     /gene="ccrA"
                     /locus_tag="VM638_05315"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HVM11877.1"
                     /translation="MHASVIRPERYGEPEDAFQVEEIPVPEVGPDDALVLVMAAGINY
                     NNVWAARGIPVDITKVHAKLGEPDDFHIGGSDASGVVYAVGENVTNVAVGDHVVVHCG
                     QWDREADALEDPMLHPSFRIWGYETNWGSFAQFTKVQAHQLMPKAPQLTWEQAAAPTL
                     VGATAYRMLLGWPPHTVQPDDVVLIWGGAGGLGCMAIQIVREMGGKAVAVVSDESKAE
                     FCRKLGAVGVIDRREFDHWGMLPHWTDEEAFAEWTKGAKAFGKAIWEALGEKRSPRIV
                     FEHPGESTIPTSMFVTDTGGMVVICAGTTGYNAVVDLRYLWMRQKRFQGSHFANDEQS
                     LAFNQLVMDGRIDPCLSRTFAFDEIGTAHQLMGENKHPEGNMAALVSAPREGLTDLP"
     gene            complement(2511..2930)
                     /locus_tag="VM638_05320"
     CDS             complement(2511..2930)
                     /locus_tag="VM638_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVM11878.1"
                     /translation="MIALDTSVAVAAFAPWHTLHRDAVATLGEGARLPAHAALETFAV
                     LTRLPPPHRAPAGAVSRWLAERFPAPWLTLAPAGYRRILEAVAGGGLGGGAIHDAALA
                     ATALEHGATLVSADRRAKPVYELIGVEVDYLPAPPAR"
     gene            complement(2927..3172)
                     /locus_tag="VM638_05325"
     CDS             complement(2927..3172)
                     /locus_tag="VM638_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HVM11879.1"
                     /translation="MKTTIDGAGRVVIPKSIRDRLGLEGGTEIEITERDGAIEIEPAA
                     TPMRLEEGEQGLVAVPDRELPPLQPDTVRETLERIRR"
     gene            complement(3192..4118)
                     /locus_tag="VM638_05330"
     CDS             complement(3192..4118)
                     /locus_tag="VM638_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVM11880.1"
                     /translation="MELTPVQERTLVELLGRDHPRPSFPRELPERLVALIERQAGGAL
                     DSLREGEQVWISKTGLADLHARCEGMYQARIMGENVFEMNLPLAVGKVVHRAIETDVI
                     GPRLPEPELAEKAIERMRRSDPEFDRFVGTLDPVDAAWLISEGARRLALFRATMPPLK
                     KQWAPIPELRIKAEVARGRLVLSGAIDLCLFRPDESAPAQATRLFVELKTGASRAEFA
                     DDLRFYALLATLRFGVPPFRVATLLLDDGSYRPEDVTEDVLEAAARRVAAAVERHVEL
                     LGDRQPTLRPGAWCRWCPRAATCPEASLPAGP"
     gene            4177..4605
                     /locus_tag="VM638_05335"
     CDS             4177..4605
                     /locus_tag="VM638_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HVM11881.1"
                     /translation="MPIYRSEAGDAALVSLEPDTDLLEGLLAAAKDLDMRAGWIQVIG
                     AAKRLAYGVYDQKTQEYRTFEEEGQFEIAAAMGNVSLKDGDPFVHLHIVATRHDGSAI
                     GGHLMPGTTVFVAEAWLQRLASETVPVREMDERRGLALWR"
     gene            4620..>5894
                     /locus_tag="VM638_05340"
     CDS             4620..>5894
                     /locus_tag="VM638_05340"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVM11882.1"
                     /translation="MATTRDPEQEQVTDPRLADLAKRTAEALQAGGEGAVSRQHERGK
                     LSARERLEILLDPGSFVETDMLARHQAHGFGIEKTRPYGDGVITGWGTIDGRKVFVYA
                     QDFTVFGGSLGETHAQKICKVMDLAMSTGAPVIGLNDSGGARIQEGAASLAGYGTIFD
                     RNVRASGVIPQISVIMGPSAGGAVYSPAVTDFIYMVEETAHMFITGPEVIKTVTGEDV
                     TFEELGGAQTHASRSGIASFIAENEEECLRQVRYLLSFLPSNNMEDPPVYASADDPER
                     LVPELNTIVPESPKEPYDMKDVIRAIADGGEFYEVFPRWAMNIVIGFTRMDGMPVGVV
                     ANQPKVLAGTLDIESSEKAARFIRFCDAFNIPLVTLVDVPGFMPGTAQEYGGIIRRGA
                     KLLYAFTEATVPKLTVITRKAYGGAYVVMNSKH"
BASE COUNT          890 a         2085 c         2069 g          850 t
ORIGIN      
        1 ggacgatcgc gaggatcccg atcaggagga accccggcag ccagagcatc gcgtcctcgg
       61 gcatgcttgg gagccccacc gggttgccct cagcgaggcg cttccacagc cacgcgtcga
      121 cgatcccgag cacgatcgcg agcttgagga ggcgacgccg gcgcgtccgc tcccggatcc
      181 gggcgacatc ggcgcgcccc gccttcacga gcttcgactg tccgagggtc gccgcaacgc
      241 tgttcgtcac cgctcccgcc gcccctgctg tctcgttcgt catgtcctgc ctccctgggc
      301 ccggggcgct cgggtctccc tcgatattcc acggcggggg gcacatccct gcccagcgct
      361 acgtaccttc ctgtatggtt ctcgggctcc gccgaccgga cacttgaggg atgccgagga
      421 agccccagtt cgagcgcgaa gacgcgcgac tcgtcggcgg gctcctgcgg gacctgcggc
      481 gcgcggctgg gttccgggcg gtgaaggacg cggcggccgc ggaaggctcc cccgcggctc
      541 gtcagacgat ctacgcctac gagcgaggcg gcctgtgccc gagcctgccg cagttcctcg
      601 agctcgtcga gttctacgcg ctgaaggccc ccgcagcaga ccccgcgatc cggcacatgg
      661 cgatcgcggc gatccaccgg gccctcgcgc tcccgatgta ccacgtgcgg agcgcgctcg
      721 agctcatcga gcggctccag cccgagccgt cccgcgcgcg ccgccgcggt ggcgccgcga
      781 gcgaggacga ggaaggatag gcgcgtgatc gaggacatcg accacgtggg catcgccgtc
      841 ggggacctgg agaccgcggt ggaacgctac cgggccgcgt acggcatcga gccggcgcac
      901 cgggagacgg tcgaggagca gggcgtcgag gaggtcctgt tcgccgtcgg gacctcctac
      961 atccagctcc tgcgcccgct cgggccggag acaccggtcg gacggttcct cgagaaacgc
     1021 ggggagggca tccaccacgt ggcctatcgc gtgcggtcgg tccgcgaggg gctcgaccgc
     1081 ctccgcgccg aggggcaccg cctgatcgac gaggagcccc ggcgggggtc gcgcggcacg
     1141 acgatcgcct tcgtccaccc gaaggccttc gacggcgtcc tgatcgagct cgtcgaagag
     1201 ggctagggca ggtcggtcag gccctcccgc ggggcgctca cgagcgcggc catgttcccc
     1261 tcggggtgct tgttctcgcc catgagctga tgggcggtcc cgatctcgtc gaacgcgaag
     1321 gtccgcgaca ggcacgggtc gatgcgcccg tccatcacga gctggttgaa ggcgagcgac
     1381 tgctcgtcgt tggcgaagtg cgagccctgg aagcgcttct gacgcatcca caggtaccgg
     1441 aggtccacga ccgcgttgta gccggtggtg ccggcgcaga tcacgaccat gccgccggtg
     1501 tcggtcacga acatcgaggt ggggatcgtc gactcaccgg ggtgctcgaa cacgatgcgc
     1561 ggcgagcgct tctcgccgag cgcctcccag atcgccttcc cgaacgcctt cgcccccttc
     1621 gtccactcgg cgaacgcctc ttcgtcggtc cagtgcggga gcatgcccca gtggtcgaac
     1681 tccctccggt cgatcacgcc gacggctccc agcttgcggc agaactcggc cttcgactcg
     1741 tcggagacga cggcgacagc cttgccgccc atctcgcgca cgatctggat cgccatgcac
     1801 ccgagtccgc cggcgccgcc ccagatcagg acgacgtcgt caggctgcac ggtgtgcggc
     1861 ggccagccga ggagcatgcg gtaggcggtg gcgccgacga gcgtcggcgc ggcggcctgc
     1921 tcccaggtga gctgtggcgc cttcggcatc agctgatggg cctggacctt cgtgaactgg
     1981 gcgaacgagc cccagttcgt ctcgtagccc cagatccgga aggacgggtg cagcatcggg
     2041 tcctcgagcg cgtcggcctc gcgatcccac tgcccgcagt gcacgacgac gtggtcgccg
     2101 accgccacgt tggtcacgtt ctcgccgacc gcgtagacga cgccggatgc gtccgacccc
     2161 ccgatgtgga agtcgtcggg ctcgccgagc ttcgcgtgga ccttcgtgat gtcgaccggg
     2221 atgccgcgcg ccgcccagac gttgttgtag ttgatgccgg cggccatcac gagcacgagc
     2281 gcgtcgtcgg gccccacctc cggcacgggg atctcctcga cctggaaggc gtcctccggc
     2341 tcaccgtacc gctccggccg gatcacgctg gcgtgcatgc gagcgggaac ctcgccgagt
     2401 ggcgggatct cgccgatctc gtacaggtcc ttggccacgg ctcctcctgg acgctcggtc
     2461 tggttactgc tcagtaggtt ataccgcggc cggggcgggc ggcaacctgc ctatcgggcg
     2521 ggcggagccg gcaggtagtc gacctcgacg ccgatcagtt cgtagaccgg cttggcacgc
     2581 cgatcggcgc tcacgagcgt cgctccatgc tcgagcgccg tcgccgccag cgcagcgtca
     2641 tggatggctc cgcctcccag accgccacct gccaccgcct cgaggatccg acggtagccg
     2701 gcaggcgcga gcgtcagcca gggtgcgggg aaccgctccg cgagccatcg gctgaccgct
     2761 ccggctggcg ctcggtgcgg gggcgggagc ctggtgagca ccgcgaacgt ctcgagcgct
     2821 gcgtgcgccg ggagacgtgc gccctccccc aacgtagcca ccgcgtcccg atggagcgtg
     2881 tgccaagggg cgaaggcggc caccgccacg ctcgtgtcga gcgcgatcac cgccggatcc
     2941 gctcgagcgt ctcccggacg gtatcgggct ggaggggcgg gagctcccgg tcgggcacgg
     3001 caaccaggcc ctgctcgccc tcctcgaggc gcatcggcgt tgcggccggc tcgatctcga
     3061 tggcgccgtc acgctcggtg atctcgatct cggtgccgcc ttccagcccc aaccggtccc
     3121 ggatgctctt cgggatgacc acacgcccgg cgccatcgat ggtggttttc atggtaggaa
     3181 aataccatcc ctcatgggcc cgcgggaagc gacgcctccg ggcaggtggc tgcgcgggga
     3241 caccaccgac accaggcacc cggccggagc gttggctggc gatctcccag gagctcgacg
     3301 tggcgctcca ccgcggcggc gacgcggcgc gcggcagctt cgagcacgtc ctcggtcacg
     3361 tcctctggtc ggtaggaccc gtcgtcgagc aggagcgtcg ccacgcggaa cggggggacg
     3421 ccgaagcgga gcgtcgccag gagggcgtag aagcgcaggt cgtcggcgaa ctccgcccgg
     3481 ctcgcgccgg tcttcagctc gacgaacaga cgggtcgcct gcgccggcgc cgactcgtcc
     3541 ggacggaaga gacagaggtc gatggcgccg ctcagcacga ggcgcccgcg ggcgacctcc
     3601 gccttgatcc gcagctcggg gatcggcgcc cactgcttct tgagcggcgg catcgtcgcg
     3661 cggaagagcg ccagccggcg cgcgccctcg gagatgagcc acgcggcgtc gaccggatcg
     3721 agcgtgccca cgaaccggtc gaactccggg tccgagcgcc gcatccgctc gatcgccttc
     3781 tcggcgagct ccggctcggg caggcgcggc ccgatcacgt cggtctcgat cgcgcggtgg
     3841 acgaccttcc cgaccgcgag cgggaggttc atctcgaaga cgttctcgcc catgatccgg
     3901 gcctggtaca tgccctcgca gcgcgcgtgg aggtcggcga ggccggtctt cgagatccag
     3961 acctgctccc cctcgcgcag cgaatcgagg gcaccgccgg cctggcgctc gatcagggcg
     4021 acgagacgct cgggcagctc gcgcgggaag ctcggccgcg gatggtcccg gccgagcagc
     4081 tcgacgaggg ttcgctcctg gacgggcgtc agctccacac ggataggcta gcccccgcca
     4141 ccgacacgat cgcggaagcg aaaggagatc cctcacatgc cgatctaccg atccgaggcc
     4201 ggggacgcgg ccctcgtgag cctcgagccc gatacggacc tgctggaggg tctcctcgcg
     4261 gctgcgaagg atctcgacat gcgcgcgggc tggatccagg tgatcggcgc cgccaagagg
     4321 ctcgcctacg gcgtctacga ccagaagacg caggagtacc ggacgttcga ggaggagggc
     4381 cagttcgaga tcgcggccgc gatgggcaac gtctcgctca aggacgggga tcccttcgtt
     4441 cacctgcaca tcgtggccac ccggcacgac ggcagcgcca tcggcggcca cctgatgccc
     4501 ggcacgacgg tcttcgtggc cgaagcgtgg ctccagcgtc tcgccagcga gacggtcccc
     4561 gtccgcgaga tggacgagcg gcgcggtctc gcgctctggc gatagactcg gcgacggcca
     4621 tggcgacgac gcgcgacccc gagcaggagc aggtgaccga cccgcgcctc gcggacctcg
     4681 cgaagcgcac ggccgaggcg ctccaggcgg gtggcgaggg cgcggtctcg cgccagcacg
     4741 agcgcgggaa gctctcggcc cgcgagcgcc tcgagatcct cctcgacccc ggttccttcg
     4801 tcgagaccga catgctcgcg cggcaccagg cgcacgggtt cgggatcgag aagacgcgcc
     4861 cgtacggcga cggcgtcatc accggatggg gaaccatcga cggccggaag gtcttcgtct
     4921 acgcgcagga cttcacggtc ttcggcgggt ccctcggcga gacgcacgcc cagaagatct
     4981 gcaaggtgat ggacctcgcg atgtcgacgg gggcgccggt catcgggctg aacgactccg
     5041 ggggcgcgcg gatccaggag ggcgcggcct cgctcgcggg ctacggcacg atcttcgacc
     5101 gcaacgtccg cgcgtccggc gtcatccccc agatcagcgt catcatgggc ccctcggccg
     5161 gcggcgccgt ctactccccg gcggtcaccg acttcatcta catggtcgag gagaccgcgc
     5221 acatgttcat caccgggccc gaggtgatca agaccgtcac cggcgaggac gtgacgttcg
     5281 aggagctcgg cggcgcgcag acgcacgcct cgcgcagcgg catcgcgagc ttcatcgccg
     5341 agaacgagga ggagtgcctg cgacaggtcc gctacctgct ctcgttcctg ccgtcgaaca
     5401 acatggagga cccgccggtc tacgcgagcg ccgacgaccc ggagcggctc gtgcccgagc
     5461 ttaacacgat cgtgccggag tcgccgaagg agccctacga catgaaggac gtgatccggg
     5521 cgatcgcgga cgggggcgag ttctacgagg tcttcccgcg ctgggcgatg aacatcgtga
     5581 tcgggttcac gcggatggac ggcatgccgg tcggcgtcgt cgcgaaccag ccgaaggtgc
     5641 tggccgggac gctcgacatc gaatcgagcg agaaggccgc gcggttcatc cgcttctgcg
     5701 acgcgttcaa catcccgctc gtgacgctcg tcgacgtgcc cgggttcatg cccgggacgg
     5761 cgcaggagta cggcgggatc atccgccgtg gggcgaagct cctctacgcg ttcacggagg
     5821 cgacggtccc gaagctcacg gtcatcaccc gcaaggccta cggcggggcg tacgtcgtca
     5881 tgaactcgaa gcac
//
LOCUS       DATLGG010000187         3033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_58934, whole genome shotgun sequence.
ACCESSION   DATLGG010000187 DATLGG010000000
VERSION     DATLGG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58934"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1066
                     /locus_tag="VM638_05345"
     CDS             410..1066
                     /locus_tag="VM638_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HVM11883.1"
                     /translation="MDRDSWRARTDILATVDPQRRQVLSVPRDLWCDPVRNRVNVAFA
                     RGGHDLLVSCLASIGIEAQHSVCLRRLAVERALEGVTVTVPVRTPLRFWYPLAPQAPI
                     EDGRKPVDFDPPEAELSGERLHQWMGARIARTGPSSDLHRIRRQQMLVVALLEQGFDF
                     TSFVRDPDLVKTSSPRVLEELRQVDASWTFEVFDRVVPAKIDDKEVLVPAPVSDAPPA
                     "
     gene            complement(1044..1451)
                     /locus_tag="VM638_05350"
     CDS             complement(1044..1451)
                     /locus_tag="VM638_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVM11884.1"
                     /translation="MRPCDPSQHRRYTTPWGIDVVVNRALLARFALACERAAREPWAV
                     RRIDSYVCRPVRGGSVYSRHAYAAAWDFFSTPPGVEPPGGVWTPDDPVPAGFARHFED
                     LGFRWGRWLPRRDDPHIEWPDDEVPELTPGERR"
     gene            complement(1460..>3033)
                     /locus_tag="VM638_05355"
     CDS             complement(1460..>3033)
                     /locus_tag="VM638_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM11885.1"
                     /translation="KVLVTNPRREGYAGWDDGANRNRLLEAAAELDPEWVLELDADER
                     IPADDAAALRRFVQDDAVPGFAYGFRVYRMIGDEQHYDRAGLWVYRLFAYEPGQRVPD
                     VLQHFVPVPTSIPRSNWLRTTVRIQHLSSLTEERRRARYAKYVEADPERAYQPDYKHL
                     LDEPGRVRAWKPRPSALPVLVDPLGKQRVNEFVLEDLDLDAPLLSAVVIARNDEERIE
                     RVLGSVVSQRCPFPFEVIAVVSGTDRTAEIVRDRFPEVTLIELEGAALPGRARNAGVE
                     VARGDYVSFPGSHVELPPGSLAARLAAHERGYPMVTGETHNGTRTRSGWASYFLDHAV
                     NLPGQPAGELVGPPAHCSYARDLLIEVGGFPTDMRAGEDTTVNLELARRGYVAYRSSD
                     VKLVHHTRCSNPLRLIRHHFSRGRAYGKILLEGRVFRGAGARRKLTRHVIVYVRQRIK
                     ATTRNVRTADPEMWKTYRRVFPLVVTAVVAAWLGLLSKLLSTPLTGRDRKARGSSAPA
                     PQGSMRGTARAGGSA"
BASE COUNT          452 a         1037 c         1085 g          459 t
ORIGIN      
        1 cgcggacgcc atcccggtcc aggtccccga gagcgatcgc gcgcggaaag gccgagggaa
       61 ccgccatccg gccgaagctc gggccctcgt cgctcggtgc cgctccggag gctggagggc
      121 tgccgaacag cacgacgacc agggctgtcg cgacgagcgc cgtccggggc ggtgaccccg
      181 aggtggcgcg tcgcgaggtc gatccgtctc gagtgcgggc cgaggacacg atgtggatgg
      241 attcgcggcc ggttacgcgg gaccctcgtc agccgtcggc ggacccgccg ccagctgatg
      301 atgcgcccgt ccccggctca gccccgttgc ggccacctcc cggcagggct agcatccggc
      361 tgcttcgagg acgacgccgg aggatgcatt ggcgaccgcg gtcggcatca tggatcggga
      421 cagctggcga gctcgaaccg acatcctcgc gaccgtcgac ccccagcggc gtcaggtcct
      481 ctcggtgccc cgcgacctgt ggtgcgatcc cgtcaggaac cgcgtgaacg tggccttcgc
      541 gcgcggtggc catgatctgc tggtctcgtg cctcgcgagc atcggcatcg aggctcagca
      601 cagcgtgtgt ctgcgacgac tcgccgtcga acgcgcgctg gaaggcgtca cggtcaccgt
      661 gccggtgcga accccgctga ggttctggta cccgctcgct ccccaagcgc cgatcgagga
      721 tgggcggaaa ccggtcgact tcgatccgcc cgaggcggag ctgtccggcg agcgtctgca
      781 ccagtggatg ggggcgcgga tcgcgcggac gggcccgtcc tcggacctgc accgcatccg
      841 tcgccagcag atgctcgtcg tcgcgctcct cgagcagggc ttcgacttca ccagcttcgt
      901 ccgcgatccc gatctcgtga agacgtcgtc tcctcgagtg ctcgaggagc tgcgccaggt
      961 ggacgcttcc tggacgttcg aggtcttcga ccgcgtcgtc ccggcgaaga tcgacgacaa
     1021 ggaggtgctg gtacctgcgc ccgtcagcga cgctcccccg gcgtgagctc ggggacctcg
     1081 tcgtccggcc actcgatatg gggatcgtcg cggcggggta gccacctccc ccagcggaag
     1141 ccgaggtctt cgaagtggcg agcgaaccct gcgggaactg ggtcgtccgg tgtccagacc
     1201 ccgccggggg gctccacccc gggcggcgtc gagaagaagt cccacgccgc ggcgtacgcg
     1261 tgccgcgagt agaccgatcc gccacggacg ggacggcaga cgtagctgtc gatcctgcgg
     1321 accgcccacg gttctctggc agcccgctcg caggcgaggg cgaaccgagc gaggagcgcg
     1381 cggttgacga cgacgtcgat gccccacgga gtcgtgtagc ggcggtgctg cgacggatcg
     1441 cagggccgca tcagcatcct caggccgatc ctccagcccg tgccgttcca cgcatcgatc
     1501 cctgcggcgc cggcgcgctc gagccacgcg ccttcctgtc ccgaccggtc aggggggtgc
     1561 tgagaagctt cgagagcagt cccagccagg ccgcgacgac ggcagtgacg acgaggggga
     1621 agacccgccg atacgtcttc cacatctcgg gatccgcggt acggacgttc cgcgtcgtcg
     1681 ccttgatgcg ctggcggacg tagacgatga cgtgccgcgt gagcttccgc cgcgcccccg
     1741 cgccgcggaa cacgcgaccc tcaagcaaga tcttcccgta cgcacgccca cggctgaagt
     1801 ggtgtcggat caggcgcagc gggttgctgc accgggtgtg gtggacgagc ttcacgtcgc
     1861 tgctgcggta cgcgacgtag ccgcggcgag cgagctccag gttgacggtc gtgtcttcgc
     1921 ccgctcgcat gtcggtgggg aacccgccca cctcgatgag caggtcgcgg gcgtacgagc
     1981 agtgagcggg cggtccgacc agctcgccag cgggctgacc tggaaggttc acggcgtgat
     2041 cgaggaagta cgacgcccac cccgatcggg tgcgtgtccc gttgtgggtc tcgccggtga
     2101 ccatcgggta cccccgttcg tgggcggcga gccgcgcggc caggctgccc gggggcagct
     2161 ccacgtgcga gccggggaac gacacgtagt cgccgcgcgc gacctcaacg ccggcgttcc
     2221 tcgcccgacc tggcagggct gcgccttcga gctcgatgag ggtcacctcg gggaagcgat
     2281 cgcgcacgat ctcggcggtc cggtcggtgc cgctcacgac ggcgatgacc tcgaagggga
     2341 aggggcatcg ctgggagacc acggagccta ggacgcgttc gatccgctcc tcgtcgttcc
     2401 gcgcgatgac gacggccgac agcaggggcg catcgagatc gaggtcctcg aggacgaatt
     2461 cgttcacgcg ttgcttcccc aagggatcca cgaggaccgg gagagccgac gggcgtggtt
     2521 tccacgctcg aacgcgaccg ggctcgtcca gaaggtgctt gtagtcgggc tggtacgccc
     2581 gttcggggtc cgcctcgacg tatttggcgt accgagcgcg gcgccgctcc tccgtcaagc
     2641 tcgagaggtg ctggatacgc acggtcgtcc gcagccagtt cgagcggggg atcgaggtgg
     2701 ggaccggaac gaagtgctgg agcacatccg gcacccgctg gcccggttcg taggcgaaga
     2761 gccggtagac ccagagacct gcgcgatcgt agtgctgctc gtctccgatc atgcggtaga
     2821 cgcggaagcc gtaagcgaac ccggggaccg cgtcgtcctg cacgaaccgg cggagcgcgg
     2881 cggcgtcatc ggcggggatg cgttcgtcgg cgtccagctc gagcacccac tccgggtcca
     2941 gctccgcggc ggcttccagg aggcggttgc ggttcgcgcc gtcatcccag cccgcgtagc
     3001 cctctcgtcg cgggttcgtc acgagcacct tca
//
LOCUS       DATLGG010000188         4655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_58967, whole genome shotgun sequence.
ACCESSION   DATLGG010000188 DATLGG010000000
VERSION     DATLGG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58967"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VM638_05360"
     CDS             complement(<1..256)
                     /locus_tag="VM638_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HVM11886.1"
                     /translation="MRTATSRGTRAVVRSPEATPLAIVGPTAAGKSALAVEIARELDA
                     EIVAVDSMTIYRGMDVGTAKPTPEQRALVRHHLLDVAEPTE"
     gene            complement(292..1593)
                     /gene="miaB"
                     /locus_tag="VM638_05365"
                     /pseudo
     CDS             complement(292..1593)
                     /gene="miaB"
                     /locus_tag="VM638_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            complement(1594..3129)
                     /gene="rny"
                     /locus_tag="VM638_05370"
     CDS             complement(1594..3129)
                     /gene="rny"
                     /locus_tag="VM638_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HVM11887.1"
                     /translation="MTVALAALAALVVGVALGFVIRRFMGQRAVSSAEVKARKLVTDA
                     EREAQDTIRRSLAEVKEEIAAMRRDAEQDIKARREESRRREEALTKREESIEVKAAQV
                     AKAERAVGDRQQRLEQTQKELERAREEHRRELERIARMTAQEAKDSLMAAMVDQAKRD
                     AMGTVREIEERAREEGEERARKIVTIAIQRVASEQTSESTVSVFTLPSEDLKGRIIGR
                     EGRNIRAFEALTGVNLIIDDTPEAVVLSSFDPVRREIARMTLDKLVQDGRIQPVRIEE
                     MFERSTKEVEEQIRRAGERALEDVGITDAHPEMVRVLGRLQYRTSYGQNVLKHLVESA
                     HVAGVMAAELGLANPALVKRCALMHDIGKALTHEVEGSHALIGAELARRLKEPPEVVH
                     AIESHHGEVEQRTVEAVLAQAADAISGSRPGARRESIEQYVKRLERLEQICTSHPGVA
                     KTFAMQAGREVRVMVKPEEVDDLSAQVLARDIAKQVEEELQYPGQIRITVVREMRAVE
                     VAK"
     gene            complement(3370..3834)
                     /locus_tag="VM638_05375"
     CDS             complement(3370..3834)
                     /locus_tag="VM638_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HVM11888.1"
                     /translation="MATSAKDRALRVLGIRARSREELRRRLAQKGHEVEEIEAALDDL
                     ESVGLVDDEEFARQLTAHHIGVRGSGRRVALAELRRAGVETEVAERAVAAGAPEDEEA
                     RAEELARGRLRRLGTLDEATAYRRLVAFLHRRGYDAETARNAARRALANSEL"
     gene            complement(3854..>4655)
                     /gene="recA"
                     /locus_tag="VM638_05380"
     CDS             complement(3854..>4655)
                     /gene="recA"
                     /locus_tag="VM638_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVM11889.1"
                     /translation="HAMDPTYAKRIGVNVDELLVSQPDTGEQALEIADMLIRSGALDI
                     IVIDSVAALVPRAEIEGEMGDSHVGLQARLMSQALRKITGALSNSGTTAVFINQLREK
                     IGVMFGSPETTPGGRALKFYSSVRLDVRKIDNLKDGTEVVGSRVRAKVVKNKVAPPFR
                     QAEFDIIYGKGISKEGSLLDVGVSLEMIKKSGAWFTYEGEQLGQGRENARQFLMEHRD
                     LAEEIERKIREAVGLTSFGEDEVVSIEEPVTDAAPTSGSSKKEPARAG"
BASE COUNT          607 a         1699 c         1580 g          769 t
ORIGIN      
        1 cctccgtggg ctcggcgacg tcgagcaggt gatggcggac gagcgcacgc tgttccggcg
       61 tgggcttcgc cgtcccgacg tccatcccgc ggtagatcgt catcgagtcg acggcgacga
      121 tctcggcgtc gagctcgcgg gcgatctcga cggcgagcgc gctcttgccc gccgcggtcg
      181 ggccgacgat cgcgagcggg gtggcctcgg gagagcggac caccgcgcgg gtgccgcggg
      241 aggtggccgt cctcatcccg cgcccgagcg agccggttgg agagcgtgcc ccgtgaggtg
      301 gtgggggtgg ccccgctcga tgcgcacctg gaggaacgtg cccggtgacg ccggctcggt
      361 gacattcacg accttgttcg tccgcgtccg tccctgcgtc ccgcccttgc gacccgcgcc
      421 ctcgacgagg atctcgtgga cccgcccttc ctcggcccgg ttcttctcga gcgagatccg
      481 ctcctgcaga gcgacgagcc gctcgaaccg ctcctgcacg acctcgcggg caacctgctc
      541 ctcgagggtc gcggcaacgg ttcccggccg cggcgagtac tggaaggtga acgcggcgtc
      601 gaaccgcgcc gcctcgacga cctgcagcgt ctcggcgaag tcgtcctcgg tctcgcctgg
      661 gaacccgacg atgatgtcgg tggtcaccgc gatctcgggg atcgcctcgc ggatccgggc
      721 gagccagccg aggtagcgct cggcgcggta ggagcggcgc atcttcccca ggatccggtc
      781 ggacccggac tgcaggggga agtggatgtg ctcgcagacg cgctcacact cggccatcgc
      841 ctcgatcacg tcctcggaga agtggtgcgg gtgcgggctc gtgaagcgga tccggcggat
      901 cccgtccacc tcgttcacgg cacgcaacag cgcgccgaac aacggccggg gcggatgacc
      961 gtcgaccgtc acgtcgcggc cataggtgtt cacgttctgc ccgaggagcg tgacctcgac
     1021 cacgccttcc cgcgcgagac cctggacctc gccgaggatc tgcccgagcg gccgggatcg
     1081 ctgcgggccg cggacgagcg gcacgatgca gaacgagcag gtgttgtcac acccgaccga
     1141 gacgctcacc cacgcgtgat gcgcgacctc gcggcgggcc ggaagcgccg acgggaacat
     1201 ctgcgtgtac tcgccgacat ccatctgcgg accctcgcgc tccgcgcggc ggagcaggtc
     1261 ggtgatctcc ggcagggcgt aggtcccgac gacgacgtcg acccacgggg cacgccgctg
     1321 gatctccccg cggtccttct gggcgaggca ccccgcgacg acgatccgca tgcgcgggtt
     1381 ccggtccttc gcctgccgca gccgtccgag gttcccgtag agccggtcgt cggcgttctc
     1441 gcgcaccgcg cacgtgttca gcacgacgac gtcggcttcc tcgacccgcg aggtctccgc
     1501 catcccgtcg gccacgagcg ttcccgcgat ccgctcggag tcgtgctcgt tcatctgaca
     1561 gccgaacgtg cggatcagga agcggccgtc cacctacttc gcgacctcca ccgcgcgcat
     1621 ctcgcggacg accgtgatcc ggatctgccc ggggtactgg agctcctcct cgacctgctt
     1681 cgcgatgtcg cgcgcgagga cctgcgcgga gagatcgtcg acctcctccg gcttcaccat
     1741 cacgcggacc tcccggcccg cctgcatcgc gaacgtcttc gcgacgccgg ggtgcgaggt
     1801 gcagatctgc tcgaggcgct cgaggcgctt gacgtactgc tcgatgctct cgcgccgtgc
     1861 gccaggtcgg ctgccggaga tcgcgtccgc cgcctgggcg aggacggcct cgaccgtccg
     1921 ctgctcgacc tcgccgtggt gcgactcgat cgcgtggacg acctccggcg gctccttcag
     1981 gcgccgcgcc agctccgcgc cgatcagcgc gtgggacccc tcgacctcgt gcgtcagcgc
     2041 cttgccgatg tcgtgcatca gcgcgcagcg cttgacgagg gccgggttcg cgaggccgag
     2101 ctcggcagcc atcacgccgg cgacgtgcgc cgactcgacg aggtgcttga ggacgttctg
     2161 cccgtaggag gtccggtact ggagccgtcc gagcacgcgg accatctcgg ggtgcgcatc
     2221 ggtgatgccg acgtcctcga gggcgcgctc ccccgcgcgt cggatctgct cctcgacctc
     2281 cttcgtggag cgctcgaaca tctcctcgat ccggaccggc tggatgcggc cgtcctggac
     2341 gagcttgtcg agggtcatcc gcgcgatctc gcgccggacc gggtcgaagc tcgagagcac
     2401 gacagcctcc ggcgtgtcgt cgatgatgag gttgacgccg gtcagggcct cgaaggcgcg
     2461 gatgttgcgg ccctcgcgcc cgatgatgcg ccccttcaga tcctccgacg gcagcgtgaa
     2521 gaccgagacc gtcgactccg acgtctgctc ggaggccacg cgctggatcg cgatcgtcac
     2581 gatcttgcgt gcgcgctcct ccccttcctc gcgggcgcgc tcctcgatct cacggaccgt
     2641 tcccatcgcg tcgcgcttcg cctggtcgac catcgccgcc atcagcgagt ccttcgcctc
     2701 ctgcgccgtc atgcgcgcga tccgctcgag ctcgcggcgg tgctcctcgc gggcgcgctc
     2761 gagctccttc tgcgtctgct cgaggcgctg ctgacgatcg ccgacggccc gctcggcctt
     2821 cgcgacctgt gctgccttta cctcgatcga ctcctcacgc ttcgtgagcg cctcttcgcg
     2881 gcgccgcgac tcctcgcgac gagccttgat gtcctgttcg gcgtcgcggc gcatcgccgc
     2941 gatctcttcc ttcacctcgg cgagcgagcg gcggatcgtg tcctgcgcct cccgctcggc
     3001 gtcggtgacg agcttgcggg ccttcacctc cgcgctcgag acggctcgct gccccatgaa
     3061 cctgcggatc acgaagccca gcgcgacgcc aaccacgagc gccgcgaggg ctgcgagtgc
     3121 gacggtcatc gttcctccct gtctgcgccg gacgaggtcc ggctcccgcc gggaggacgg
     3181 cgaggacgcg caagaggaac ggcgccgtgc gcctaggggc cggctctcct cctgctcgcg
     3241 tgaatatcgt cggggaacac gtcgatgtcc tctggcccgc caggcgggtt cggttgtcgg
     3301 tttgcgttca caaatgtact agccgacccg tcagagcgca acagatggcg agagatccct
     3361 cggcgaaggt cagagctccg agttcgcgag ggcgcggcgg gcggcgttgc gggcggtctc
     3421 ggcgtcgtag ccgcggcggt ggaggaacgc caccaagcgc cggtacgcgg ttgcctcgtc
     3481 cagcgtcccg agacggcgca ggcgaccgcg agcgagctcc tcggcccggg cctcctcgtc
     3541 ctcgggggcg ccggcggcaa ccgcccgctc ggccacctcc gtctcgacgc cggctcgccg
     3601 cagctcagcg agcgcgacgc gtcgcccgga tccacgcaca ccgatgtggt gcgcggtcag
     3661 ctggcgggcg aactcctcgt cgtcgacgag cccgaccgac tcgaggtcgt cgagggcggc
     3721 ctcgatctcc tccacctcgt gccccttctg ggcgaggcgg cggcgcagct cctcgcggct
     3781 ccgggcccgg atgccgagca cgcgcagggc ccggtccttc gcggaggtgg ccaccgcctc
     3841 gcctcctgct cgctcagccg gcgcgggccg gctccttctt cgaggagccc gatgtggggg
     3901 cggcatcggt cacgggctcc tcgatggaga cgacctcgtc ttccccgaac gaggtcagtc
     3961 ccacggcttc acggatcttg cgctcgatct cctcggcgag gtcccggtgc tccatcagga
     4021 actgtcgcgc gttctcgcgt ccctggccga gctgctcgcc ctcgtaggtg aaccaggcgc
     4081 ccgacttctt gatcatctcg agcgagacgc cgacgtcgag cagcgagcct tccttggaga
     4141 tccccttgcc gtagatgatg tcgaactccg cctggcggaa cggcggcgcg accttgttct
     4201 tcacgacctt cgcccgcacg cgcgacccga cgacctcggt tccgtccttg aggttgtcga
     4261 tcttccgcac gtccagccgg acgctcgagt agaacttcag cgcccgcccg ccgggggtgg
     4321 tctcgggcga gccgaacatc acgccgatct tctcgcggag ctggttgatg aagaccgccg
     4381 tggtaccgga gttgctgagc gcaccggtga tcttgcgcag cgcctggctc atcaggcggg
     4441 cctgcaggcc gacatggctg tcgcccatct cgccctcgat ctcggcgcgc ggcacgagcg
     4501 cggccaccga gtcgatcacg atgatgtcga gcgcgccgga gcggatcagc atgtcggcga
     4561 tctcgagggc ctgctcgccg gtgtccggct gggacacgag cagctcgtcg acgttgacgc
     4621 cgatgcgctt cgcatacgtc ggatccatcg cgtgc
//
LOCUS       DATLGG010000189         3612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_44328, whole genome shotgun sequence.
ACCESSION   DATLGG010000189 DATLGG010000000
VERSION     DATLGG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44328"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VM638_05385"
     CDS             complement(<1..1357)
                     /locus_tag="VM638_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVM11890.1"
                     /translation="MLRTLTETAQELRERRVSPVELVEGALDRASRWQPVINAFSQVH
                     AEEALREASRQADALARGEDPGPLAGVPVAVKDLFDVAGWETSGSSLAYRGSVARADA
                     EAVTRLRRAGAILIGKTNQHELACGGTNSVSAHGPARNPWDPARITGGSSGGSAAAAA
                     ARIVPLALGTDTGGSIRIPASFCGVAGLKPTYGLLPTDGVMPLAPSLDTVGALGARVE
                     DVALGHAVLAGLGAGWVETTTGSIAGIRVAILGGFFEKRVDAQVLEAVGSAAAELERS
                     GAEVRSMDPGAVEDAPDVWSILTMSEFAAGHGRLLRRPETLYPMTRGSMEWGMQRTAV
                     DYLHARERARAIRDAFLRLLDEADVLLAPATIFPAPPHGADEVEVAGDLVGIHRGGAG
                     WLTRPISLTGLPALSLPVGRSREGLPLGGQLVARDGEEALLLRVGLAYQRATDHHVAA
                     PT"
     gene            complement(1398..2420)
                     /locus_tag="VM638_05390"
     CDS             complement(1398..2420)
                     /locus_tag="VM638_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVM11891.1"
                     /translation="MQVFDASEARERRPSMALVWTALLAVWIVWGSTYLAIRVGVRTI
                     PPLLMASARFLVAGTILYLIVRRRDPRRLSSQHWRSAFLIGGALLLGGNGLVSIAEQS
                     VPSGIAALIIASVPLFLASGDRVFFGKRLSWVAVVGLVVGFAGVALLVGQSVGGRFDL
                     GGMLLLIGASISWASGSLYARAAPQPDNPVLGAAMQMMAGSLILVVVAGLTGEFARLD
                     LGGISLESFLGLVYLIVFGSWVGFVAYVWLLKHVSVSLVGTYAYVNPVVAVLLGWAIL
                     GEAVTARTLVAGGIVIIGVALIVAAREPKPLAGDPAGAKPPVRHRLSLRRSRRVHRSD
                     RSLASR"
     gene            2510..3031
                     /locus_tag="VM638_05395"
     CDS             2510..3031
                     /locus_tag="VM638_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVM11892.1"
                     /translation="MFALEHERVLDETGWRILAELQEDGRISLSELGRRVALTPPAVA
                     ERVRRMEEAGIIKGYRAEIDRSRVGLPITVYIRWTATGNDCAALGEVAREIPEIVECH
                     RVTGEESYVVKAAVRSVGHLEELINRLMPHGETKTSMVLSSAVEHRPVERDHVATDEP
                     EAATPIRGRRRSA"
     gene            3031..>3612
                     /locus_tag="VM638_05400"
     CDS             3031..>3612
                     /locus_tag="VM638_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM11893.1"
                     /translation="MAVRVGLGLFTGQVPPESGRSFADEYHDIIELARLGESLGFDSV
                     WVSEHHGAGDGYLPSLLPVLGAIAAVTDRVRLGTGVLLTPFHHPLRLAEDAAVVDQLS
                     GGRLILGLGLGWREEEFRMFGVPIRERVGRTLETVEILRKAWTGERFTHRGRAFPLED
                     VRVTPPPARDDGIPIYVGGHVEAACRRAGAVGEP"
BASE COUNT          554 a         1291 c         1322 g          445 t
ORIGIN      
        1 gcgtcggcgc ggccacgtgg tggtccgtcg cccgctggta ggcgaggccg acgcgcagca
       61 gcagggcctc ctccccgtcg cgcgcgacga gctggccgcc gagcggaagc ccctcgcgtg
      121 agcgaccgac cgggagcgac agcgccggca ggcccgtcag gctgatcggc cgcgtcagcc
      181 agcccgcccc gccgcggtgg atcccgacca ggtcgccggc gacctcgacc tcatccgcgc
      241 cgtgcggcgg tgccgggaag atcgtcgccg gcgcgaggag aacgtcggcc tcgtcgagca
      301 accgcaggaa cgcgtcgcgg atcgcccgtg cgcgttcccg cgcgtggagg tagtcgaccg
      361 cggtgcgctg catcccccac tccatcgagc cccgggtcat cggatagagg gtctcgggcc
      421 gtcggagcag gcgcccgtgg cccgcggcga actcggacat cgtcaggatg ctccagacgt
      481 cgggtgcgtc ctccacggcg ccgggatcca tggagcgcac ctcggcgccg ctgcgctcga
      541 gctcggcggc ggccgacccc accgcctcca gcacctgcgc gtcgacgcgc ttctcgaaga
      601 accctccgag gatcgccacg cggatcccgg cgatcgaccc ggtggtcgtc tcgacccatc
      661 ccgcccccag cccggcgagc accgcatgcc ccagggcgac gtcctccacg cgcgcgccga
      721 gcgctccgac cgtgtcgagc gagggcgcga ggggcatgac cccgtcggtc ggaagcaacc
      781 cgtaggtggg cttcaagccg gcgaccccgc agaaggaggc ggggatgcgg atcgatccac
      841 ccgtgtcggt gccgagggcc agcggcacga tgcgcgcggc ggcggcggcg gccgacccgc
      901 cgctcgagcc cccggtgatc cgcgccgggt cccacgggtt gcgcgccgga ccgtgtgccg
      961 agaccgagtt cgtgcccccg cacgcgagct cgtgctggtt cgtcttgccg atcaggatcg
     1021 cgccggcgcg tcgtaggcgt gtcaccgcct cggcgtccgc acgcgcgacg ctcccccggt
     1081 aggcgaggct gctgcccgac gtctcccagc ccgcgacgtc gaagaggtcc ttgacggcga
     1141 ccgggacgcc ggcgagaggc ccggggtcct ccccgcgcgc gagcgcatca gcctgccgtg
     1201 acgcctctcg cagcgcctcc tccgcgtgga cctggctgaa ggcgttgatc accggctgcc
     1261 agcgggacgc tcggtcgagc gcgccttcga cgagctccac cggcgagacg cgccgctcgc
     1321 gaagctcctg cgccgtctcc gtcagggtcc gcagcacggc gtcaggctat aggagcggag
     1381 cgagcgaggg gagggggcta ccgggaagcc agcgagcgat ccgaccggtg gacccgccgc
     1441 gatcggcgga gcgaaagacg gtggcggacc ggcggcttcg cccctgcggg atcgcccgcc
     1501 agcggcttcg gctcccgtgc cgcgacgatc agcgcgaccc cgatgatgac gatcccgccc
     1561 gcgacgagcg tccgagccgt caccgcctcc ccgaggatcg cccagccgag cagcacggcg
     1621 acgaccgggt tcacgtaggc gtacgtcccg acgagcgaga ccgagacgtg cttcaggagc
     1681 cagacgtagg ccacgaaccc gacccacgac ccgaagacga tgaggtagac gaggccgagg
     1741 aaggactcga gcgagatgcc gccgaggtcg agtcgtgcga actcgcccgt caggccggcg
     1801 acgaccacga ggatcaggct ccccgccatc atctgcatcg cggcgccgag caccgggttg
     1861 tcgggctgcg gggcggcccg ggcgtagagc gacccgctcg cccaggagat cgacgcgccg
     1921 atcagcagga gcatccctcc gagatcgaac cggccgccga ccgactgccc gacgaggagc
     1981 gcgacgcccg cgaagccgac cacgaggccc accaccgcga cccacgacag gcgcttgccg
     2041 aagaagacgc ggtccccgct cgcgaggaac agcggaaccg aggcgatgat gagcgcggcg
     2101 atcccggatg ggaccgactg ctcggcgatc gagacgaggc cgttgccgcc gagcaagagc
     2161 gcgccgccga tcaggaaggc cgagcgccag tgttgggagg agaggcgccg ggggtcgcgg
     2221 cggcgcacga ttaggtagag gatcgtcccc gcgacgagga acctcgcgga ggccatgagc
     2281 aacggtggga tcgtgcggac accgacgcgg atcgcgagat acgtcgaccc ccagacgatc
     2341 cacacggcga ggagcgcggt ccacaccagc gccatcgacg gacgccgctc tcgcgcctcg
     2401 ctggcgtcga acacctgcat ggctttatga taggggtgtc cttcgcggat gtgaatggca
     2461 gatcatagag atgtggctgg ggtcactttg aaacctgaac cggatggagg tgttcgcttt
     2521 ggaacatgaa agggtcctcg acgaaacggg ctggaggatc ctcgcggagc tccaggagga
     2581 cggccgcatc tcgctcagtg agctcggacg ccgcgtcgcg ctcacgcccc cggcggtcgc
     2641 cgagcgggtg cgtcggatgg aggaggccgg gatcatcaag gggtaccgcg ccgagatcga
     2701 ccggtcccgc gtcgggctcc cgatcacggt ctacatccgc tggacggcga ccgggaacga
     2761 ctgcgccgcg ctgggagagg tcgcccggga gatccccgag atcgtcgagt gccaccgcgt
     2821 gaccggcgag gagtcctacg tcgtgaaggc ggcggtgcgc tccgtggggc acctcgagga
     2881 gctgatcaac cggctgatgc cccacggcga gacgaagacc tcgatggtcc tcagctcggc
     2941 ggtcgagcac cggcccgtcg agcgggacca cgtcgccacc gacgagccgg aagcggccac
     3001 gccgatccgc ggccggcgcc gctccgcctg atggcggtcc gggtcggact cgggctgttc
     3061 acggggcagg taccgccgga gagcggccga tcgttcgccg acgagtacca cgacatcatc
     3121 gagctcgccc ggctcggaga gagcctcggc ttcgactccg tctgggtgag cgagcaccac
     3181 ggcgcgggcg acggctacct cccctccctc cttccggtcc tcggcgcgat cgccgccgtc
     3241 accgatcggg tccggctcgg gaccggggtc ctgctgacgc cgttccacca tcccctccgg
     3301 ctcgcggagg acgccgccgt cgtggaccag ctctccggcg ggcggctcat cctcggactg
     3361 ggcctcgggt ggcgcgagga ggagttccgg atgttcgggg ttccgatccg ggagcgcgtc
     3421 gggcgaacgc tcgagacggt ggagatcctg cggaaggcgt ggacgggcga acggttcacc
     3481 caccgcggcc gcgcgttccc gctcgaggat gtgcgcgtca cgccgcctcc ggcccgagac
     3541 gatgggatcc cgatctacgt ggggggtcac gtcgaggctg cgtgccgtcg cgcgggggcg
     3601 gtgggtgaac cg
//
LOCUS       DATLGG010000190         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_138583, whole genome shotgun sequence.
ACCESSION   DATLGG010000190 DATLGG010000000
VERSION     DATLGG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138583"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VM638_05405"
     CDS             complement(<1..1062)
                     /locus_tag="VM638_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVM11894.1"
                     /translation="MASNDGPAEGADLRRLEFLAEIGATLVSLDPKRILGDLTRVVVP
                     ELADWALAYLVEEGGRIRRVAMGHADASKAELAEEIEQEFWIVPDAPVGLPRVIRTGE
                     AEFYPRATAALLAADVQEPERMEPMLRELGVRSWICIPLSARGRVLGALSLVYAESGR
                     NYTPADLALAKTLAQRASLAVDNALLVSDLEFQQVLLRSQSEATIDGILVVDPEGKII
                     SFNQRFVEMWGIPPEVIKTGSDEAAIEFVVDQLEDPEAFIARIRHLYAHPEERSRDVL
                     HLRDGRTFDRWSAPLTGPDGAPYGRAWYVRDVTDFARVEEILRENQRRAELLAEAGSL
                     FAASLDDRVIAKGLAELVAR"
     gene            complement(1117..3159)
                     /locus_tag="VM638_05410"
     CDS             complement(1117..3159)
                     /locus_tag="VM638_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVM11895.1"
                     /translation="MRIVVVGGGPAGVYAALLLKKADRSHDITVVERNPEGATYGWGV
                     VFSDRTLGEFREADAVTFERITQGFVTWDAIDVRIGGKVIRSGGHVFAGIARKRLLAI
                     LAERAAELGVELRFDTEIPDPSELFDADLVVGADGVRSGVREAWHERFRPRLSEGRAR
                     YIWFGTPLALDSFTFSFRPTEHGLFQAHAYPFDGRMSTFIVECPEDVWRGAGLDRADE
                     AGSIAFCEDVFADDLRGAPLLSNRSAWISFVTVKNATWHHANVVLIGDAAHTAHFSIG
                     SGTKLAMEDAISLAAAFERHGQDVDTALADYESERKPIVERFQDAAAESRTYFEETAR
                     WLHVEPEQFAFHLLSRSGRIDYDVLRLRDAAYVDGVDRWFARRDGGIAPPPLFTPFEV
                     GPLRLANRVVATTDRPLDGAALLLTPIAAVSPEGRITTDDLTTYRPEDEARIAAVVRE
                     ARDSGAAVGLRIGHAGRRGATEPRARGLDRPLAEGWELLAPTAEPYTAVSPTPRAMTE
                     DDAARIVGGFAAAAAVATRAGVDLLEIHMAQGYLLGSFLSPLTNRREDRYGQERLLLP
                     LAVVDAVRSAWDGPLAVALNAADGVPGGTEPPEAVRVAAAMRERGVDLVEVLAGYTTA
                     RMRPRYGPAFLAPLADVVRNDARVPVLCGGGVFTTGRANTMLAGGRADLVVMSPPR"
     gene            complement(3159..>3851)
                     /locus_tag="VM638_05415"
     CDS             complement(3159..>3851)
                     /locus_tag="VM638_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVM11896.1"
                     /translation="CYGALDPFDSLTAAANVTERVRLVTMVAVAPLRTAEALVDAAEG
                     VQRASAGRLVLGMAVGARPEDYVAAGVPHRGRGARFDDQLTRLPGLWSEEPPEILVGG
                     TDDRAFARVTRHADGYVHGGGPPRAFARAAEKARAAWIDGGRPGEPRLWGQSYFALGD
                     EEEVERGRAYMLDYYAFTGPFARRIAEGLLTTPQAIAHQIRGYAEAGCDELVLLPALA
                     RIEQVDRLAEVL"
BASE COUNT          537 a         1401 c         1406 g          507 t
ORIGIN      
        1 ccgtgcgacg agctccgcga gacccttcgc gatgacccgg tcgtcgaggg aggcggcgaa
       61 cagcgagccg gcctccgcca gcagctccgc ccggcgctgg ttctcccgca ggatctcctc
      121 gacccgtgcg aagtcggtga cgtcgcggac gtaccacgcg cggccgtacg gcgcgccgtc
      181 cggccccgtc agcggcgcgc tccagcggtc gaaggtgcgt ccgtcgcgca ggtgcaggac
      241 gtcgcggctc cgctcctcgg ggtgcgcata gaggtggcgg atacgagcga tgaacgcctc
      301 gggatcctcg agctggtcga cgacgaactc gatcgcggct tcgtcggacc cggtcttgat
      361 cacctcgggc gggatgcccc acatctcgac gaaccgctgg ttgaacgaga tgatcttccc
      421 ctccggatcg accacgagga tgccgtcgat cgtcgcctcg ctctgcgagc gcaggagcac
      481 ctgctggaac tcgaggtccg agacgagcag ggcgttgtcg accgcgaggc tcgctcgttg
      541 cgcgagggtc ttcgccagcg cgaggtcggc gggcgtgtag ttgcgacccg actcggcgta
      601 gacgagcgag agggcgccga ggacgcgtcc gcgcgccgac aacgggatgc agatccacga
      661 tcggaccccg agctcgcgaa gcatcggctc catccgctcg ggctcctgca cgtcggcggc
      721 gaggagcgcc gcggtcgcgc gtgggtagaa ctccgcctcg ccggtgcgga tcacgcgcgg
      781 gagcccgacg ggggcgtcgg gcacgatcca gaactcctgc tcgatctcct cggccagctc
      841 cgccttcgag gcgtccgcat ggcccatggc gacgcggcgg atgcggccgc cctcctcgac
      901 gaggtacgcg agggcccagt ccgccagctc cgggacgacg acgcgcgtga ggtcgccgag
      961 gatgcgcttc gggtcgaggg agacgagggt cgcgccgatc tccgcgagga actcgaggcg
     1021 gcggagatcg gcgccctccg ccgggccgtc gttcgatgcc accaagcccc tcctccccgt
     1081 gcttgcttcc cccggaaagg ataccgctgc tcgctctcag cgcgggggcg acatcacgac
     1141 gagatccgca cgtccgccgg cgagcatcgt gttcgcgcgc ccggtggtga acacgccgcc
     1201 gccgcacagg acgggaacgc gcgcgtcgtt ccggacgacg tcggccagcg gcgccaggaa
     1261 ggcggggccg tatcggggcc gcatcctggc cgtcgtgtac cccgcgagca cctcgacgag
     1321 gtcgacaccc cgctcgcgca tcgccgccgc cacgcggacc gcctcgggtg gctcggtccc
     1381 cccggggacg ccgtccgcgg cgttcagcgc gacggcgagg ggcccgtccc acgccgagcg
     1441 cacggcgtcg acgaccgcga gcggcaggag cagccgctcc tggccgtagc ggtcctcccg
     1501 gcggttcgtg agcggtgaga ggaagctgcc gagcaggtag ccctgcgcca tgtggatctc
     1561 cagcaggtcg acccccgcgc gcgtggcgac cgccgcggcc gccgcgaacc cgcccacgat
     1621 gcgggcggcg tcgtcctcgg tcatcgcgcg tggggtgggg ctgacggccg tgtagggctc
     1681 cgccgtgggc gcgaggagct cccagccctc ggcgagcgga cggtcgaggc ctcgcgcacg
     1741 cggctcggtg gcgccccgcc ggccggcgtg cccgatgcgg agcccgaccg ccgcacccga
     1801 gtcgcgcgcc tcccggacga cggccgcgat ccgcgcctcg tcctccggac gatacgtggt
     1861 gaggtcgtcg gtggtgatcc gtccctccgg cgagacggcg gcgatcggcg tgagcagcag
     1921 cgccgcgccg tcgagtggcc ggtcggtcgt ggcgaccacg cggttcgcga gccgcagcgg
     1981 acccacctcg aacggcgtga acagcggggg cggggcgatg ccgccgtcgc ggcgggcgaa
     2041 ccagcggtcc accccatcga cgtaggccgc gtcgcgcagg cgcaggacgt cgtagtcgat
     2101 ccgcccgctt cggctgagga ggtggaacgc gaactgctcg ggctcgacgt ggagccagcg
     2161 tgccgtctcc tcgaagtacg tccggctctc ggcggccgca tcctggaacc gctcgacgat
     2221 cggcttccgc tcggattcgt agtccgcgag ggccgtgtcc acgtcctgcc cgtgacgctc
     2281 gaaggccgcg gcgagggaga tcgcatcctc catcgcgagc ttcgtccccg atccgatcga
     2341 gaagtgagcg gtgtgcgccg cgtccccgat gagcaccacg ttggcgtgat gccaggtcgc
     2401 gttcttcacc gtgacgaacg agatccaggc cgatcggttc gagagcaggg gcgccccgcg
     2461 caggtcgtcg gcgaagacgt cctcgcagaa ggcgatgctg ccggcctcgt ccgcgcgatc
     2521 gaggcccgcc ccgcgccaga cgtcctcggg gcactcgacg atgaaggtgc tcatccgtcc
     2581 gtcgaacgga tacgcatgcg cctggaagag gccgtgttcc gtcgggcgga acgagaacgt
     2641 gaacgagtcg agcgcgagcg gcgtcccgaa ccagatgtag cgggcgcgac cctcggagag
     2701 ccgtggccgg aagcgctcgt gccatgcctc gcgaacgccg ctccggacgc cgtcggcacc
     2761 gacgacgagg tcggcgtcga agagctccga gggatcgggg atctccgtgt cgaagcggag
     2821 ctcgaccccc agctccgccg cccgttccgc gaggatcgcg agcaggcgct tgcgcgcgat
     2881 cccggcgaag acgtgcccgc ccgaacggat caccttcccg ccgatgcgca cgtcgatcgc
     2941 gtcccacgtc acgaagccct gcgtgatgcg ctcgaacgtc acggcgtcgg cctcgcggaa
     3001 ctcgccgagg gtccgatcgg agaacacgac cccccagccg tacgtggcgc cttcggggtt
     3061 ccgctcgacc acggtgatgt cgtggctccg atcggccttc ttcagcagca gcgccgcgta
     3121 cacacccgcg ggtccgcccc ccacgacgac gatccgcatc agaggacctc cgcgagccgg
     3181 tcgacctgct cgatgcgggc gagcgccggg agcaagacga gctcgtcgca tccggcttcg
     3241 gcgtagccgc ggatctggtg ggcgatcgcc tgcggcgtgg tcagcaggcc ctccgcgatc
     3301 cggcgggcga aggggccggt gaacgcgtag tagtcgagca tgtacgcgcg cccgcgttcg
     3361 acctcttcct cgtcgccgag cgcgaagtag ctctgccccc acaggcgcgg ctcgccgggc
     3421 cggccgccgt cgatccacgc ggcgcgcgcc ttctccgcag cccgagcgaa ggcgcgcggt
     3481 ggtccgcccc cgtggacgta gccgtccgcg tgccgcgtga cgcgggcgaa ggcgcggtcg
     3541 tcggtcccgc cgacgaggat ctccggcggc tcctcgctcc agagccccgg cagacgcgtg
     3601 agctggtcgt cgaagcgggc gccgcgcccg cggtgcggga ccccggccgc gacgtaatcc
     3661 tccgggcgtg ccccgacggc catcccgagg acgagccgcc cggcggaggc acgctgaacc
     3721 ccctcggcgg cgtcgacgag ggcctcagcc gtccgcaggg gcgcgacggc gaccatcgtc
     3781 accaggcgga cgcgctcggt cacgttggcc gcggcggtga gggagtcgaa cgggtcgagg
     3841 gcgccgtagc a
//
LOCUS       DATLGG010000191         3822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_138719, whole genome shotgun sequence.
ACCESSION   DATLGG010000191 DATLGG010000000
VERSION     DATLGG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138719"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VM638_05420"
     CDS             <1..367
                     /locus_tag="VM638_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11897.1"
                     /translation="FRFFAGRDPFLPLIVADIGTGGRETTEAQTGDDGTGTVEEPAPD
                     AEQRAQDGGGTSVGGHRVTLVDISGNRAQVTVDGETYTVREGERFAGSFRLVSIDGSC
                     ATFQHGDESFTLCEGGERK"
     gene            508..1575
                     /locus_tag="VM638_05425"
     CDS             508..1575
                     /locus_tag="VM638_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVM11898.1"
                     /translation="MDHHARRRRLLTRLDDLGIDALLVTHLPNVRYLTGFTGSNGQLL
                     IAANHAVFLTDPRYEEQARHEAPDVERATYRLDFPSSFAEISGDLGLARVGFEAASVS
                     YGLYERLRRTGVELVPTTGEVERLRRVKDPDELALIEEAQRATDDALERVLPKLVEGR
                     NEREVALDLEHEMRALGAEEAAFRSIVAFGESAAEPHHVPGERTLRRGDIIKLDFGAR
                     VQGYCADMTRTVALGAPSRDLVEIHDLVRRAQAEGIEAVRPGAVGGEVDRVAREVIAH
                     AGQRERFGHSLGHGIGLEVHEAPTLRRGSEDVLEPGTVVTVEPGVYIPGVGGVRIEDM
                     VVVTETGARPLPSAPRELQIL"
     gene            1589..2158
                     /gene="efp"
                     /locus_tag="VM638_05430"
     CDS             1589..2158
                     /gene="efp"
                     /locus_tag="VM638_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017242230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HVM11899.1"
                     /translation="MSVSTNDLKTGMTLSLDGGELWTVLDFLHHKPGKGQAVVRTKLR
                     NLRTGSVQDRTFKADEKVGLARLDRREMQYLYREGEELVFMDNETYEQHHVPSEVAGE
                     ATKYLVEGATAILPIHEGLAIGVELPAAVVLTVSKTDPGVKGDRVSGATKLAELETGI
                     STQVPLFIEEGDRIKVDTRTGGYLTREKE"
     gene            2163..2564
                     /gene="nusB"
                     /locus_tag="VM638_05435"
     CDS             2163..2564
                     /gene="nusB"
                     /locus_tag="VM638_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVM11900.1"
                     /translation="MTRRHEARRRAVALLYEADVGGRSPLDLLEERRSLGHDVPRFTE
                     ELVRGVAERIEELDRLIGTHAEGWTVPRLAAVDRAVLRLACHEILHRDEVPAAVAVDE
                     AVEAAKELSTEDSGRFVNGILGRIVRGCAAS"
     gene            complement(2571..3020)
                     /locus_tag="VM638_05440"
     CDS             complement(2571..3020)
                     /locus_tag="VM638_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11901.1"
                     /translation="MRGFLGVVLTLALWPAPAGANAASGCDAFPWVQTYQGWGPAANA
                     GRTVTVVYVADCTASDGAIEASGTATVHRGRTTDGSVLAVHPFRHRATWSSGGGRFPW
                     WTCVEEAEYVWELGEWYTFRATAAGGSWSLEVSPGPDGTLTWSHGAC"
     gene            complement(3080..3295)
                     /locus_tag="VM638_05445"
     CDS             complement(3080..3295)
                     /locus_tag="VM638_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HVM11902.1"
                     /translation="MIAFLQGIGAPELIIILVILLLLFGARKLPEMARSMGRASKEFK
                     QGLRDGADEEPMGAKDLEHPAKDRRTD"
     gene            3489..>3822
                     /locus_tag="VM638_05450"
     CDS             3489..>3822
                     /locus_tag="VM638_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HVM11903.1"
                     /translation="MAPDTATRVLGTPDIRRAVTRIAHEILEGNRGAEDLVLVGIAAR
                     GDDLARRLATEIERIEGTEVPVGVLDITFYRDDIGLRAEAPEVHETRIPFDVTGRTVV
                     LVDDVLYTG"
BASE COUNT          578 a         1336 c         1397 g          511 t
ORIGIN      
        1 gttccgcttc ttcgcgggtc gggacccctt cctcccgctg atcgtggccg acatcggcac
       61 cggcggacgg gagacgaccg aggcccagac cggcgacgac gggacgggaa ccgtcgagga
      121 gccggccccc gatgccgagc agcgggctca ggacggcggc ggcacgagcg tcggcggaca
      181 ccgcgtgacg ctcgtcgaca tcagcggcaa cagggcgcag gtgaccgtgg acggggagac
      241 ctacaccgtg cgggaggggg agcgcttcgc cggcagcttc cggctcgtct cgatcgacgg
      301 ctcctgcgcg accttccagc acggagacga aagcttcacc ctctgcgagg ggggtgagcg
      361 caaatgacgc cccgcgcggc gtcccggctc ccggcgccct gacctcgccg ggagccaaca
      421 cgtccggcgg tccgcgatct ggggggaccg gccgccgggt tcgggaggcg gtccttcggg
      481 gccgcctccc atctatgatg gggcaccgtg gaccaccacg cgcgccgccg gcggctgctg
      541 acccgcctcg acgacctcgg gatcgacgcc ctgctcgtga cccatctccc gaacgtccgc
      601 tacctgaccg ggttcacggg gtcgaacgga cagctcctga tcgcggcgaa ccatgcggtc
      661 ttcctgaccg acccccgcta cgaggagcag gcgcgccatg aggcgcccga cgtcgagcgg
      721 gcgacctacc ggttggactt cccctcctcc ttcgccgaaa tcagcggcga cctcggcctg
      781 gcgcgcgtcg gcttcgaagc cgcctccgtc agctacggcc tgtacgagcg gttgcggcgg
      841 acgggcgtcg agctcgtccc gaccaccggt gaggtcgaac gcctacgccg cgtgaaggac
      901 ccggatgagc tggcgctgat cgaggaggcg cagcgggcga ccgacgacgc gctcgagcgc
      961 gtcctgccga agctcgtcga ggggcggaac gagcgagagg tggccctcga cctcgagcac
     1021 gagatgcgcg cgctcggggc ggaggaggcc gcgttccggt cgatcgtcgc gttcggtgag
     1081 agcgcggcgg agccccacca cgtgccgggg gagcggaccc tgcggcgggg cgacatcatc
     1141 aagctcgact tcggcgcccg cgtgcagggc tactgcgccg acatgacgag gacggttgcg
     1201 ctcggggcgc cgtcccggga cctcgtcgag atccacgacc tcgtgcgccg tgcgcaggcc
     1261 gaggggatcg aggccgtgcg gccgggggcc gtcggaggtg aggtcgaccg cgtggcgcgc
     1321 gaggtgatcg cccacgccgg tcagcgcgag cggttcggcc actccctggg acacgggatc
     1381 ggtctcgagg tgcacgaggc gccgaccctg cggcgggggt ccgaagacgt cctcgagccc
     1441 gggacggtcg tcacggtcga gcccggggtg tacatcccgg gggtcggcgg ggtccgcatc
     1501 gaggacatgg tggtggtcac ggagacgggg gcgagacccc tcccgtcggc gccccgcgag
     1561 ctgcagatcc tgtgacgaaa ggagcgacgt gagcgtctcg acgaacgacc tgaagacggg
     1621 gatgaccctc tcgctggacg ggggtgagct ctggaccgtg ctcgacttcc tccatcacaa
     1681 gccggggaag ggacaggccg tcgtccggac gaagctgcgc aacctgcgga cgggatccgt
     1741 gcaggaccgc accttcaagg cggacgagaa ggtgggtctc gcgcgtctcg accgtcggga
     1801 gatgcagtac ctctaccgcg agggagagga gctcgtcttc atggacaacg agacgtacga
     1861 gcagcaccac gtgccgtccg aggtcgccgg ggaggcgacg aagtacctcg tcgagggggc
     1921 gaccgcgatc ctcccgatcc acgagggtct cgcgatcggc gtcgagctgc cggcggcggt
     1981 cgtgctgacg gtatcgaaga cggacccggg ggtgaagggc gaccgggtga gcggcgcgac
     2041 gaagctcgcc gagctcgaaa ccgggatctc gacgcaggtg ccgctgttca tcgaggaggg
     2101 ggaccgcatc aaggtggaca cgcgcaccgg cggctacctc acgcgcgaga aggagtgagc
     2161 gcgtgacccg tcgccacgag gcacggcggc gggctgtcgc gctcctctac gaggcggacg
     2221 tcggcggccg atcgccgctg gatctcctcg aggaacgacg ctcgctcggc cacgacgttc
     2281 cgcgcttcac cgaggagctc gtccgcggcg tggccgagcg gatcgaggag ctcgaccggc
     2341 tcatcgggac gcacgccgaa ggctggaccg ttccgcggct cgcggcggtc gaccgcgcgg
     2401 tcctgcggct cgcgtgccac gagatcctcc accgagacga ggtgccggct gcggtcgcgg
     2461 tcgacgaggc ggtcgaggcg gcgaaggagc tctcgacgga ggactctggt cggttcgtga
     2521 acgggatcct cggccggatc gtgcgggggt gcgccgcgag ctgaggtccc tcagcaggcc
     2581 ccgtgggacc acgtgagggt cccgtcgggc cccggggaca cctcgagcga ccagctcccg
     2641 cccgcggcgg tcgcgcggaa cgtgtaccac tcgccgagct cccagacgta ctcggcctcc
     2701 tcgacgcacg tccaccacgg gaagcgcccg ccaccgctgg accaggtagc ccggtgccgg
     2761 aacgggtgga cggcgaggac gcttccgtcg gtcgtccggc cccggtggac ggtcgcggtg
     2821 cccgacgctt cgatcgcgcc gtccgacgcg gtgcagtccg ctacgtagac gacggtgacg
     2881 gttcggcccg cgttcgcggc ggggccccag ccctggtagg tctggaccca cgggaaggcg
     2941 tcgcagcccg aggccgcgtt cgcgccggcg ggagccggcc agagcgcgag cgtcagcacg
     3001 acgccgagga aaccccgcat gcccagatca tcggcacgat gaagctacgg gtgaatagtc
     3061 agaacggagg gttctgccgc tagtcggtgc gacggtcctt cgccgggtgc tcgaggtcct
     3121 tcgctcccat cggctcttcg tccgcaccgt cgcggagccc ctgcttgaac tccttcgagg
     3181 cgcgacccat cgagcgggcc atctcgggaa gcttccgggc cccgaacagc aggagcagga
     3241 tcacgaggat gatgatgagc tccggcgcac cgatgccctg gaggaacgcg atcacgtcga
     3301 cccctttcgt cttgccgaaa gcctacaccg cgccgccgcc tcgtggtatc cttccgcgca
     3361 gcttcccttt aagtccggtc ccgtgaggcc gggaaggaga gagagcacga tgcgagacac
     3421 cgcggctcca ccccgtccgg gtggggcttt ctcttcgtct ccttccggcc tcccgctcgg
     3481 ggggcccgat ggcgcctgac accgccaccc gcgtcctcgg cacgcccgac atccgccgcg
     3541 cggtgacccg catcgcccac gagatcctcg agggcaaccg cggcgccgag gacctcgtcc
     3601 tcgtcgggat cgccgcccgc ggggacgacc tcgcgaggcg cctggccacc gagatcgagc
     3661 ggatcgaggg gacggaggtc cccgtcgggg tcctcgacat caccttctac cgcgacgaca
     3721 tcggcctgcg cgccgaagcc cccgaggtcc acgagacccg gatccccttc gacgtgaccg
     3781 gccgcacggt cgtgctcgtc gacgacgtcc tctacaccgg cc
//
LOCUS       DATLGG010000192         5919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_212139, whole genome shotgun sequence.
ACCESSION   DATLGG010000192 DATLGG010000000
VERSION     DATLGG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212139"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VM638_05455"
     CDS             complement(<1..706)
                     /locus_tag="VM638_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVM11904.1"
                     /translation="MIGTTGRSGGHDPQAASDLASVGETLEELYHDAPCGYISSLPDG
                     RIVRVNETFLRWTGYEEDALLGRRLQDLLTPGGRIYHETHYAPLLRMQGSVREIALDL
                     LCADGRTLPVLMNSVLRVDERGEPALVRTVVLDATERRGYERELLRARDEAEQAEHRA
                     QELARTLQASLIPPGPPQIPGLDVAAIYRPAGDGAEVGGDFFDVFPLDDSTWGITIGD
                     VEGKGVEAAALTAFVRY"
     gene            complement(703..1503)
                     /locus_tag="VM638_05460"
     CDS             complement(703..1503)
                     /locus_tag="VM638_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVM11905.1"
                     /translation="MDVLARNNVRVSGRPDGQPIVFAHGFGCDQNMWRLVVPAFEDRY
                     RIVLFDHVGFGGSDLSAWSLGRYASLDGYARDILEICDALELDDVIFVGHSVSAMIGV
                     LAVREAPGVFDSLVLVGPSPRYINDDGYVGGFSREDIEGLLESLSSNYLGWSKQLAPV
                     IMGNQDRPHLGAELTESFCRTDPAIAEHFARLTFLSDNREDLSKVEHRALVLQCSDDV
                     IAPTSVGEYVHERMPNSEIVHLRATGHCPNLSAPEETIDAIERFLAAG"
     gene            1578..2018
                     /locus_tag="VM638_05465"
     CDS             1578..2018
                     /locus_tag="VM638_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11906.1"
                     /translation="MRVVRVLALGTTLVVACVAPARTAQDYRADAAETAKQVAAVLAS
                     VVLTIEVAQGEAATAPYLSLRLSESEAAASAAVATFRELQPPTPGSDRLRETLLERLD
                     TAVEGIEETRILVRRRAFDQLPPLEGDLRALVAELRGIEAELRG"
     gene            2015..3244
                     /locus_tag="VM638_05470"
     CDS             2015..3244
                     /locus_tag="VM638_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HVM11907.1"
                     /translation="MKKILTLALGILTAIGGFMDIGDLVTDALIGARFGLNLVWVTVV
                     AVIGITAYSEMAGRVAAVTERTVFDLVRERLGATAGLLNLSASYFVTALTLVAELCGV
                     ALAIQLATDVHYLLWVPLVAFLAFLVFWKMPFQPMERIYGVLGLSLFVFVIAIWQLDP
                     DWGRMMHDVTHPQIPPGEGISTFAFYAIVMLGAQMTPYEVFFFSSGVVEHGWSAEKLG
                     EIRVNNLIGYPIGGILAIGIQVAAYLVLQPRGIEVAHLSETVLPVVVAFGKLGLALAI
                     IGIFAATFGSVLETLLSSGYTVAHHFGWRWGKFEAPVNASRFSVLVIVTLLGATAVAL
                     TAIDPIKLTIYAVYLGAATLPFTYIPILIVANDERYMGEHVNGRLANTVGTIYVFVVS
                     AAAILALPLLIITKAGA"
     gene            3241..3627
                     /locus_tag="VM638_05475"
     CDS             3241..3627
                     /locus_tag="VM638_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11908.1"
                     /translation="MSEETLDAGLALLDRQVVDIDGKLVGKVDDLELAIRRGGEIEVE
                     AILMGPQALGPRLGGRVGGWIRRLGATLADAEEPIRVPMKHVKELGVRITLSIKVTDI
                     ERLLDVEQWLRDHVIRKIPGGRDASE"
     gene            3614..3937
                     /locus_tag="VM638_05480"
     CDS             3614..3937
                     /locus_tag="VM638_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11909.1"
                     /translation="MRASDLLHTELRDPHTGRPTQIRDLRLERMEGKTIRLRLTEVVL
                     GGGALAERFGYGRGPVRGPWLVKVVLDRLRRRHTDTVAWREVLEVRGEKVKVRRKLLN
                     EGDES"
     gene            4114..4314
                     /locus_tag="VM638_05485"
     CDS             4114..4314
                     /locus_tag="VM638_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11910.1"
                     /translation="MNVVEMGKAGLAGSKGKLVHKVSKPVARRTGMSRDQVEAIVGGI
                     FLTLLAWQVVGTLRRVWAASGR"
     gene            complement(4344..4838)
                     /locus_tag="VM638_05490"
     CDS             complement(4344..4838)
                     /locus_tag="VM638_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11911.1"
                     /translation="MTRSIIAAPLFALLLAAAGCGGAAEQEGSPDPTAAPTAAPTTAA
                     AEGTTYVGVEYEFQGEDQLASGQQTITLENGGEQPHELQLMLLEEGRTSADIEALFEA
                     DDPSQGIPEWVTQVGGTYTDVGETASFDAELEAGTYAMVCFIPDEDGVPHAAKGMIKQ
                     VTVA"
     gene            complement(4927..5808)
                     /locus_tag="VM638_05495"
     CDS             complement(4927..5808)
                     /locus_tag="VM638_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HVM11912.1"
                     /translation="MENAYKALRPLLFSLAYRMTGSVGEAEDIVQEAFLRFHRARAGG
                     EEIASTKGYLVTIVTRLSIDHSRSARVQRERYVGTWLPEPILNEEVPDVAASAELAES
                     LSLAFLVLLETLSPVERAAFLLHDVFGYPYVQVAEVIGKTEENARQLAARARRHIEAR
                     RPRFEASKEERDALARAFFQAAQEGHVERLISLLADEAVAYADGGGKAPAASQPIQGA
                     ERVARFLVALGAVAMRRGVRVRLVEANGQPAILAVEPDGRVGSVMALQIADGRIHAIA
                     SVVNPDKLVHLTEGHPG"
BASE COUNT          882 a         2004 c         2086 g          947 t
ORIGIN      
        1 tgtagcgcac gaacgccgtc agcgccgctg cttcgacacc cttcccctcg acgtcaccga
       61 tcgtgatccc ccacgtgctg tcgtcgaggg ggaagacgtc gaagaagtcg ccgcccacct
      121 cggcgccgtc gccggcggga cggtagatgg ccgccacatc gaggccgggg atctgcgggg
      181 ggcccggtgg gatcaagctg gcctggagcg ttcgggccag ctcctgtgcg cgatgctccg
      241 cctgctcggc ctcgtcgcgg gcgcgcagga gctcacgctc gtagccgcgg cgctccgtcg
      301 catcgagcac caccgtccgc accagcgccg gttcgccgcg ctcgtccacc cgcaggaccg
      361 agttcatcag gaccggcagc gtgcggccgt cggcgcagag caggtcgagc gcgatctcgc
      421 ggacgcttcc ctgcatccgc aagagcggcg cgtaatgcgt ctcgtggtag atccgtccgc
      481 cgggcgtcag gaggtcctgg agccggcgcc cgagcagcgc gtcctcctcg tagccggtcc
      541 agcggaggaa cgtctcgtta acgcggacga tcctcccgtc ggggagcgac gagatgtagc
      601 cgcacggagc gtcgtggtag agctcctcga gggtctcgcc gaccgaggcg aggtcggatg
      661 ccgcctgcgg gtcatggccc ccagaccggc cggtcgttcc gatcaccccg ccgcgaggaa
      721 gcgctcgatg gcgtcgatcg tctcctcggg cgcgctcagg ttggggcagt gcccggtcgc
      781 gcgcaggtgg acgatctcgc tgttcggcat ccgctcgtgg acgtactcgc cgacgctcgt
      841 cggcgcgatc acgtcgtcgg agcactggag gacgagcgcg cgatgctcga ccttggacag
      901 gtcctcccgg ttgtcggaga ggaacgtgag gcgggcgaag tgctcggcga tcgccgggtc
      961 cgtgcggcag aacgactcgg tcagctcggc accgaggtgt gggcggtcct ggttgcccat
     1021 gatcaccggc gcgagctgct tcgaccaccc gaggtagttg ctcgacagcg actccaggag
     1081 gccttcgatg tcctcgcgcg agaacccgcc gacgtacccg tcgtcgttga tgtagcgggg
     1141 cgacggaccg acgaggacca gtgagtcgaa gaccccggga gcctcgcgga cagcgagcac
     1201 gccgatcatc gcgctgaccg agtgcccgac gaagatgacg tcgtccagct cgagcgcgtc
     1261 gcagatctcg aggatgtccc gcgcgtaccc gtcgagggag gcgtaacgcc ccagcgacca
     1321 ggcactgagg tccgatccgc cgaagccgac gtgatcgaac agcacgatgc gatagcggtc
     1381 ctcgaaggcg gggacgacca accgccacat gttctggtcg catccgaagc cgtgagcgaa
     1441 gacaatcggc tgcccgtcgg gccgtccgga aacgcgcacg ttgttgcggg cgaggacatc
     1501 cacgccacca tggtacccat gttcctgggt gatccggccg gggtgaaggc agagcgaccg
     1561 gcgggtaggc tggccgagtg cgggtcgtgc gggtcctggc gctcgggacg acgctggtgg
     1621 tcgcttgcgt cgccccggcg cggacggccc aggattaccg ggcggacgcc gccgagaccg
     1681 cgaaacaggt ggcggccgtc ctcgccagcg tcgtgctgac gatcgaggtg gcgcagggag
     1741 aagcggcaac cgccccgtac ctctcgctcc ggctgagtga gtcggaggcg gccgcgtctg
     1801 ccgcggtcgc gacgttccgt gagctccagc ctccgacgcc cgggtcggat cggctgcgcg
     1861 agacactcct cgagcggctg gacaccgccg tcgaggggat cgaggagacg cggatcctgg
     1921 ttcggcggcg agcgttcgac cagctgccac cgctcgaggg ggacctccgc gcgctcgtgg
     1981 cggagctacg agggatcgag gcggagctgc ggggttgaag aagatcctca cgctcgcgct
     2041 cgggatcctg accgcgatcg gcgggttcat ggacatcggc gacctggtca cggacgcgct
     2101 gatcggggcg cgcttcgggc tcaacctcgt gtgggtgacg gtcgtggcgg tgatcggcat
     2161 cacggcatat tcggagatgg cggggcgggt cgcggccgtg accgagcgaa ccgtcttcga
     2221 cctcgtgcgt gagcgtctcg gtgccaccgc gggactgctg aacctgagcg cctcgtactt
     2281 cgtcactgca ctcacgctcg tggcggagct gtgcggggtc gcgctcgcga tccagctcgc
     2341 caccgacgtg cactacctgc tgtgggtgcc gctcgtcgcg ttcctcgcgt tcctcgtctt
     2401 ctggaagatg cctttccagc cgatggagcg catctacggc gtcctcgggc tctcgctgtt
     2461 cgtcttcgtg atcgcgatct ggcagctcga cccggactgg ggacggatga tgcacgatgt
     2521 cacgcacccg cagatccctc cgggcgaggg catctcgacc ttcgccttct acgcgatcgt
     2581 catgctgggc gcgcagatga ctccgtacga ggtgttcttc ttctcctccg gcgtcgtgga
     2641 acacggctgg tcggcggaga aactcgggga gatcagagtg aacaacctca tcggctatcc
     2701 gatcggcggg atcctcgcga tcgggatcca ggtcgccgcc tacctggtgc tgcagccacg
     2761 cgggatcgag gtcgcgcacc tgagcgagac cgtcctgccg gtcgtcgtgg cgttcgggaa
     2821 gctgggcctc gccctcgcga tcatcggcat cttcgcggcc acgttcggct cggtcctgga
     2881 gaccctgctc tcgtccgggt acacggtcgc gcaccacttc ggatggcggt ggggcaagtt
     2941 cgaggcaccg gtgaacgcct cgcggttctc cgttctcgtg atcgtgacgc tcctcggggc
     3001 aacggcggtc gccctcaccg cgatcgaccc gatcaagctg acgatctacg ccgtgtacct
     3061 cggggccgcg accctcccgt tcacctacat cccgatcctc atcgtcgcga acgacgagcg
     3121 gtacatgggc gagcacgtga acggccgcct cgcgaacacc gtcggcacga tctacgtctt
     3181 cgtcgtgtcc gccgcggcga tcctcgcgct ccccctgctg atcatcacga aggccggagc
     3241 atgagcgaag agacgctgga cgctgggctc gctctcctcg accgtcaggt ggtggacatc
     3301 gatgggaagc tcgtggggaa ggtggacgac ctcgagctcg cgatccgcag aggcggtgag
     3361 atcgaggtcg aggccatcct catgggcccg caggccctgg gcccccggct cggcggcagg
     3421 gtcggcggct ggatccgccg actcggggcg accctcgccg acgcggagga gccgatccgg
     3481 gtgccgatga aacacgtgaa ggagctcggc gtccgcatca cgctctcgat caaggtgacg
     3541 gacatcgagc gcctgctcga cgtcgagcag tggctccgcg atcacgtgat ccggaagatc
     3601 cccggaggac gcgatgcgag cgagtgacct cctgcacacc gagctccgcg atccgcacac
     3661 cggccggccg acgcagatcc gcgacctgcg cctcgagcgg atggagggca agaccatccg
     3721 cctccgcctc acggaggtcg tgctcggggg cggtgcactc gccgagcgct tcggctatgg
     3781 acggggaccg gtcaggggcc cgtggctcgt gaaggtggtc ctcgatcggt tgcgccggcg
     3841 tcacacggac accgtggcct ggcgggaggt cctcgaggtg cgcggagaga aggtcaaggt
     3901 tcgcaggaag ctcctcaacg aaggcgacga gagttgatca acggtctcat catcgccgcg
     3961 gcgtggctgg agttcctcgt cctcgtaggc atcctcttct tcttcatctt gcgccgcgga
     4021 gaggagcggt gagctcgcgt agccctcggg ggtccgggta tgtaccaccg aaggcggtat
     4081 agcgaacgat gaccgacgcc aggaggtggc ctcgtgaacg tggtggagat gggcaaggca
     4141 gggctggccg gaagcaaggg gaagctcgtc cacaaggtgt ccaagcccgt ggcccggcgg
     4201 accgggatgt cccgggatca ggtcgaggcg atcgtcggcg ggatcttcct gaccctgctc
     4261 gcttggcagg tggttgggac cctgcgacgc gtgtgggcgg ccagcgggcg ctgagctgag
     4321 cgacgtccag cgccccggtc gtgctacgcg accgtgacct gcttgatcat ccccttcgcc
     4381 gcgtgcggga cgccgtcttc gtccgggatg aagcacacca tcgcgtaggt cccggcctcg
     4441 agctccgcgt cgaagctcgc ggtctccccg acgtccgtgt aggtgccacc cacctgcgtc
     4501 acccactcgg ggatcccctg ggaggggtcg tccgcctcga agagcgcctc gatgtccgcg
     4561 gaggtgcgcc cctcctcgag gagcatgagc tgaagctcgt gcggctgctc tcccccgttc
     4621 tccagggtga tcgtctgctg ccctgaagcg agctggtcct cgccctggaa ctcgtactcc
     4681 acgccgacgt aggtggtgcc ttcggcggcc gccgtcgtcg gggctgccgt cggggccgcc
     4741 gtcgggtcag gcgacccttc ctgctcggcg gcgcccccgc atcccgccgc ggccagcaac
     4801 agggcgaaca gtggtgcagc gatgatcgat cgggtcacgc tcgtctcctc tcggtcggtg
     4861 gatcccgggc gctcttaccc cgccgagccc ctcggcgaac gccgatcggg ggcgcaggcg
     4921 ccgggatcac cctgggtgtc cttcggtgag gtgcaccagc ttatcgggat tcaccaccga
     4981 cgcgatcgcg tggatccgcc cgtccgcgat ctggagcgcc atgaccgatc ccacgcggcc
     5041 atcgggctcg acagcgagga tcgcgggttg gccgttcgcc tccaccaggc gaacccggac
     5101 ccccctgcgc atcgcgaccg ccccgagcgc gaccaggaag cgggccacgc gctcggcgcc
     5161 ctggatcggc tgggaggcgg ccggcgcctt ccccccgccg tcggcgtacg cgacggcctc
     5221 atcggccaag agggagatga gccgctcgac gtgcccctcc tgggcggcct ggaagaacgc
     5281 gcgagcgagc gcatcgcgct cctccttgga tgcctcgaag cgaggccgtc gtgcctcgat
     5341 gtggcggcgg gccctcgcgg cgagctggcg agcgttctcc tcggtcttgc cgatcacctc
     5401 ggccacctgg acgtagggat agccgaagac gtcgtgcagc aggaaggccg cccgctcgac
     5461 cggggagagc gtctccagca ggacgaggaa cgcgagggag agcgactccg cgagctcggc
     5521 ggacgccgcg acgtcgggga cctcctcgtt caggatgggt tccggcaacc aggtcccgac
     5581 gtaccgctct cgctggaccc gcgccgaccg cgagtgatcg atcgacagcc tcgtgacgat
     5641 ggtcacgagg tagcccttcg tggacgcgat ctcctcgcct ccggcgcggg cgcggtggaa
     5701 cctgaggaac gcctcctgga cgatgtcctc cgcctcgccc acgctgccgg tcatccggta
     5761 ggcgagcgag aacagcagcg gccgtagcgc cttgtacgcg ttctccatcg gtggtgccct
     5821 tcctcacccc cccagacgga tctcggaccc ggatcgtgac agaaggatgt cacgcgcgat
     5881 gcccctcacc cgtctgaggg gtgggaacgg aaggaggag
//
LOCUS       DATLGG010000193         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_59332, whole genome shotgun sequence.
ACCESSION   DATLGG010000193 DATLGG010000000
VERSION     DATLGG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59332"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VM638_05500"
     CDS             complement(<1..406)
                     /locus_tag="VM638_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HVM11913.1"
                     /translation="MAKSNTPARTKTSAASKRSSSRNAGGLDGFFKISERGSTIRTEV
                     VAGFATWLTMAYILFVNPAILGFQGIPDLQAQGLQFNQVLTVTALVAGVITILMGVWA
                     NYPFAIAAGLGLNAFVAFTLVVGQGLSYPEAMG"
     gene            complement(500..1747)
                     /locus_tag="VM638_05505"
     CDS             complement(500..1747)
                     /locus_tag="VM638_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11914.1"
                     /translation="MRGKIRRHDHDPAAVALGVALLVATAVPAAAQTPLRLPTEADAV
                     AIASGSQTILYGPEGIERVLPGPVAAREQVLVELSPGGGIGRVSVDQQLTVTGLGDFR
                     FRIPGPARDVVPLPASEGEPGLRRGSMIWQGFSPGERRLAARAELHPEQEAARLPVRV
                     AELSITVDGRPLLPGERATGTMEIRLRLENQTAIPVQIAGARTDRRTTRIALEQVTDL
                     IEAGERPRPGQGGLPRALNARGDVGSRTRPVHAWFELTGSIRLPNLEPEMVQADPPAA
                     VTSTPDGVGLRTRLGPDGLRLRLRAAVEDAPPPSIDLTLVPAVPSLDGRSLPADPLDR
                     LLLLLWEVARLRQYDAYLGNPDPTGPAETSYRYVLAAVRDGAEQPPARRALPSTFGPL
                     GLAVALLALALLLLSGILLWRHS"
     gene            complement(1702..2088)
                     /locus_tag="VM638_05510"
     CDS             complement(1702..2088)
                     /locus_tag="VM638_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11915.1"
                     /translation="MRTLALVATIVLVGCGGPSLPACGPPPPADPGVELPDGFPAIAD
                     LTLTSSREAGPSLIVEGYLPHAIPAGFERASAAFEASAGYDVTKDEVEQVDAEVFFAS
                     EDSTGQVKLQQTCAGRSDVTITIRPL"
     gene            complement(2085..>2407)
                     /locus_tag="VM638_05515"
     CDS             complement(2085..>2407)
                     /locus_tag="VM638_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11916.1"
                     /translation="EEVGVTTSAGETVVAPPSLAMYAYDFVRVLAAAVEAAQGPEPAR
                     VLEFLEQVDVKGANGDERGFNEKSHESVVDDDVYFAEYRDMVFVPVDDDPLSSTLDVI
                     QQAR"
BASE COUNT          349 a          871 c          877 g          310 t
ORIGIN      
        1 cccccatcgc ctcggggtag ctgaggccct gccccacgac caacgtgaac gcgacgaacg
       61 cgttcaaccc gagccccgcg gcgatcgcga aggggtagtt cgcccagacg cccatcagga
      121 tcgtgatgac cccggcgacg agcgccgtca ccgtgaggac ctggttgaac tggagcccct
      181 gtgcctggag gtcggggatg ccctggaacc cgaggatcgc ggggttcacg aacaggatgt
      241 aggccatcgt cagccaggtg gcgaacccgg caacgacctc cgtgcggatc gtggaacccc
      301 gctcggagat cttgaagaac ccgtccaagc cgcccgcgtt gcggctcgag gacctcttgg
      361 acgccgccga cgtcttcgtc cgcgccggcg tgttcgactt cgccatgggc acccctccct
      421 cgtcgtgcgc cggccgagcc ggacgcgtgg cggggaaggc aggagcatga tagggaccgg
      481 cgcgcgcggc gcgcaagcat caggagtggc gccagagcag gatcccgctg agcaggagga
      541 gggcgagcgc gagcagcgcg acggcgagcc cgaggggtcc gaaggtgctc gggagggcgc
      601 ggcgcgctgg aggttgctcc gccccgtcac gcacggcggc gaggacgtat cggtacgacg
      661 tctccgcggg ccccgtgggg tcggggttcc cgaggtacgc gtcgtactgc cgcaagcggg
      721 cgacctccca gagcaggagg aggaggcggt cgagcgggtc cgccgggagg ctcctcccgt
      781 cgagggaggg gacggccgga acgagggtca gatcgatcga gggaggcggg gcatcctcga
      841 cggccgcccg gagccgcagc cgaagcccgt cgggtcccag acgggtccgc agcccgacgc
      901 cgtccggggt cgacgtgacg gccgcgggcg gatccgcctg gaccatctcc ggctcgaggt
      961 tcgggagccg gatcgaaccc gtgagctcga accacgcgtg cacggggcgg gtccgcgagc
     1021 ccacatcgcc gcgggcgttc agcgcgcgcg gaagaccgcc ctggcccggg cgaggccgct
     1081 cgccggcctc gatcaggtcg gtgacctgct cgagcgcgat gcgcgtcgtc cggcgatccg
     1141 tgcgcgcccc ggcgatctgg acggggatcg ccgtctggtt ctcgagccgc agacggatct
     1201 ccatcgtccc ggttgcccgc tccccgggga gcagcggacg cccgtcgacg gtgatggaca
     1261 gctcggcgac gcggaccggg aggcgcgccg cctcctgctc cgggtggagc tcggcccgtg
     1321 cggcgaggcg ccgctcaccg ggcgagaacc cctgccagat catggacccg cgccgcagcc
     1381 cgggctcccc ctccgatgcg ggcaggggga cgacgtctct ggccgggccc gggatccgga
     1441 accggaaatc ccccagtccg gtgaccgtga gctgttggtc gacgctgacg cgaccgatcc
     1501 ccccgcccgg ggagagctcg acgagcacct gctcgcgagc ggcgaccggc ccgggcagga
     1561 cccgctcgat cccctcgggc ccgtagagga tcgtctggga tccgctcgcg atcgcaacgg
     1621 cgtccgcctc ggtcggcagc cgcagaggcg tttgcgccgc ggcgggcacg gcggtcgcca
     1681 ccaggagggc gaccccgagc gctacagcgg ccggatcgtg atcgtgacgt cggatcttcc
     1741 cgcgcatgtc tgctgcagct tcacctgccc cgtgctgtcc tcgctcgcga agaagacctc
     1801 cgcgtccacc tgctcgacct cgtccttcgt gacgtcgtag cccgccgacg cctcgaacgc
     1861 cgccgaggcc cgctcgaagc ccgccgggat ggcgtggggc aggtagccct ccacgatgag
     1921 cgatggcccc gcctcgcgcg aggacgtgag ggtgaggtcg gcgatggcgg ggaagccatc
     1981 cggcagctcc acgcccggat cggccggcgg tgggggcccg cacgccggca gcgacggccc
     2041 gccgcacccg acgagcacga tcgtcgccac cagcgcgagc gtcctcatcg cgcctgctgg
     2101 atcacgtcga gcgtcgacga cagcggatcg tcgtccacgg ggacgaagac catgtcccga
     2161 tactccgcga agtagacgtc gtcatccacg acgctctcgt ggctcttctc gttgaacccg
     2221 cgctcgtcgc cgttggctcc cttgacgtcg acctgttcca ggaactcgag gacccgcgcc
     2281 ggctccggcc cctgggccgc ctcgaccgct gcggccagca cgcggacgaa gtcataggcg
     2341 tacatcgcca ggctcggcgg cgcgacgacc gtctcgccgg ccgacgtggt cacgccgacc
     2401 tcctcgg
//
LOCUS       DATLGG010000194         5873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_227981, whole genome shotgun sequence.
ACCESSION   DATLGG010000194 DATLGG010000000
VERSION     DATLGG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227981"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VM638_05520"
     CDS             <1..674
                     /locus_tag="VM638_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HVM11917.1"
                     /translation="ALQPDAYRMRAVDLVCVENTSQAGGGSVLSPEALGAIRAAADAV
                     GVPLYLDGARIFNAAAASGTEVGAFAAEAEALMFSVSKGLGAPVGSLLCGPAPLIEEA
                     RRIRLQLGGGWRQAGVLAAAALVALEEGPPLLPGDHERARRLAEGIAEATPGAVDPDR
                     VETNIVYLDVQDGAGVVARLSERGVRAGFVGGRVRMVTHRDVDDRGIDAALEAWRAVS
                     ARRGA"
     gene            complement(652..1485)
                     /locus_tag="VM638_05525"
     CDS             complement(652..1485)
                     /locus_tag="VM638_05525"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HVM11918.1"
                     /translation="MRLGAHVPTRGRITNAVAHAVERGAEALQVFISNPRAWAPPALT
                     AELAAEFRAAAAEADVGPLVAHSSYLINIASPAEGFRRRSVDLARRELEAVAALGGAG
                     LVVHSGAAGAETPRPEAIARAAESVRRIAEDAPAPVILELMAGGAGTVASTLPQAAEL
                     FEAVGRDEIRLCLDTCHLFAAGYRLDEPGGVEELFAELRDLGLAERLAVLHANDARDP
                     RGSRRDRHWHIGRGGIGEDGFRGILSRPELEGRPVLIETPGEPEDDRRNLAELRRLAG
                     R"
     gene            complement(1493..3316)
                     /gene="pknB"
                     /locus_tag="VM638_05530"
     CDS             complement(1493..3316)
                     /gene="pknB"
                     /locus_tag="VM638_05530"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HVM11919.1"
                     /translation="MIETTVDGRYQIVARIAAGGMGEVFRARDPVLDRDVALKLLHAT
                     LAGDTAFIDRFRREARASAMLNHPNIVHVHDWGQAGDTYFMVMEFVRGPNLRTVLSQH
                     GPLQPAQAVEVLQQVLAALEHAHAQGIVHRDVKPENILITTDGLVKVADFGLARALAE
                     SRVSTAPGTVTGTVQYLAPEQIQGEVADQRTDLYAAGIALYEVLTGRVPFTGETSVAI
                     AYKHLQERVPAPSAANPMVPPSLDRAVLGATEPDRERRTPDARSMRAALAASLPELPP
                     APSLAEVVRSVPSGEEAEADRAATVTIPRAESPRKRRRRRFRRVLRWATALALLTTSA
                     WASWTYAIPHIEVPDLAGMTVDAASTEAEARGLTITVERQVFSPDVPAGEIVSQDPPP
                     GDNARRGATLAIVVSRGPELVEVPKVEGRRLARAEQILAEAKLEVRVTRDFHDEVERG
                     RVVSQSPAGGATLEAGETVELVVSRGRPPVEVPDVRGRSTADATALLEAAGLGVRLLE
                     EFSTEVARGRVIRQEPAAGKTVTRGASVTIVVSKGPQRFPMPDVIGMQEEDAVRRLGR
                     LGLEVSVVRLPGSSGDRVVGQNPRRGETVEAGSQVKIYVGG"
     gene            3395..4174
                     /locus_tag="VM638_05535"
     CDS             3395..4174
                     /locus_tag="VM638_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11920.1"
                     /translation="MAQQGSLDEELAALEGRVAAGETDRRALGFSSFLRRVKRDPEAR
                     ERLAERVGAVDRRLFEARKRVIPIPVGLTILLAQTIVLAAVVPVALALARGMDRPRPG
                     LAGLLLVGAAVGLSVSVHGPAHWIAGRTMGMRFVGWFLGGPFRIQPGLKVDQASYLRA
                     DPTSRAAMHAAGAIASKVAPFAVFGVAYVQHARAGWELVPEWSLWAILGFGALQIVTD
                     VVWSTRRSDWMRVRRELRIARAEQGAVLSSRGDPGSSGQPG"
     regulatory      4149..4241
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4301..5134
                     /locus_tag="VM638_05540"
     CDS             4301..5134
                     /locus_tag="VM638_05540"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVM11921.1"
                     /translation="MASTDIRTDPLLIAGRSFSSRLLVGTGKFGSHEAMRDALDASGA
                     EVVTVALRRVDLDATGGPDILEFIDPDRYLLLPNTSGAVDADEAVRLARLARAAGMPP
                     WVKLEVTPEPRYLLPDPVETLRAAEVLVADGFTVLPYMGADPILAKRLEEAGCATVMP
                     LGSWIGSNQGLRTRDAVAIIVEQASVPVVVDAGLGAPSHAAACMELGADAVLVNTAIA
                     VASDPVAMARAFALGVEAGRRAFLAGRAPELEAAEASSPLTGFLDKLATSQTDPAVTE
                     P"
     gene            5131..>5873
                     /locus_tag="VM638_05545"
     CDS             5131..>5873
                     /locus_tag="VM638_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVM11922.1"
                     /translation="MRVERLADLAAPIGERDGFVEPWPLRTFAHALAALPLDDLVAVA
                     NDASPGRVDRALSTDAAARTLEDVAALLSPAAAERLEDVAAASRALTLARFGRTMHLY
                     APLYLSNECLTTCGYCGFAKNLPVARKTLGEDETLEEARHLVREGYRSLLLLTGEHER
                     LTGVAFLERHVRALAPLVPSIALEVQVWGEEDYRRLVAAGTEGIVIYQETYDPRRHLE
                     VHLAGRKRDYRWRLLGPERAARAGARRVG"
BASE COUNT          706 a         2104 c         2280 g          783 t
ORIGIN      
        1 cggcgctgca gccggacgcc taccgcatgc gcgcggtcga cctcgtctgc gtggagaaca
       61 cgagccaggc ggggggtggg agcgtcctgt ctcccgaggc cctgggcgcg atccgggcgg
      121 cggcggacgc cgtcggtgtg ccgctctacc tcgacggcgc gaggatcttc aacgccgccg
      181 ccgcgagcgg gaccgaggtg ggggcgttcg cggcggaggc cgaggcgctg atgttctcgg
      241 tgtcgaaggg actgggggcg ccggtgggtt cgctgctgtg cggcccggct ccgctgatcg
      301 aagaggcgcg gcggatccgg ctccagctcg gcggcgggtg gcggcaggcg ggcgtgctcg
      361 cggccgccgc gctcgtcgcc ctcgaggagg ggccaccgct gctgccgggc gaccacgagc
      421 gggcgcggcg cctggcggag gggatcgcgg aggcgacgcc gggggccgta gatccggacc
      481 gggtcgagac gaacatcgtc tacctcgacg tccaggacgg ggccggggtg gtcgcgcggc
      541 tgtccgagcg cggcgtgcgt gccgggttcg tcggcggccg cgtgcggatg gtgacgcacc
      601 gcgacgtcga cgaccgcggg atcgacgcgg cgcttgaggc ctggcgcgcc gtcagcgccc
      661 ggcgaggcgc ctgagctcgg cgaggttccg ccggtcgtcc tccggctcgc ccggcgtctc
      721 gatcagtacc ggtcgtccct cgagctccgg gcggctgagg atcccgcgga acccgtcctc
      781 gccgatcccg ccccggccga tgtgccagtg ccggtcgcgc cgggagccac gcgggtcgcg
      841 cgcgtcgttc gcgtggagga ccgcgagccg ctccgccagc ccgaggtcac ggagctcggc
      901 gaacagctcc tcgacgcctc cgggctcgtc gagccggtac cctgcggcga agaggtggca
      961 ggtgtcgagg cagagccgga tctcgtcgcg cccgaccgcc tcgaacagct cggccgcctg
     1021 cggcagcgtg gacgcgacgg tcccggcgcc gcccgccatg agctcgagga tcaccggagc
     1081 gggcgcgtcc tccgcgatcc gccgcacgga ctcggcggct cgggcgatgg cctccggccg
     1141 aggcgtctcc gccccggccg cccccgaatg cacgacgagg cccgcgccgc ccaacgctgc
     1201 gacggcctcg agctcgcgcc gggcaaggtc gaccgatcgc cgccggaacc cctcggcggg
     1261 ggaggcgatg ttgatcaggt aggagctgtg ggcgacgagc gggccgacgt ccgcctccgc
     1321 tgcggccgct cggaactccg cggcgagctc cgcggtcagg gccggtggcg cccaggcgcg
     1381 cgggttcgag atgaagacct ggagcgcctc ggccccgcgc tcgaccgcgt gcgcgaccgc
     1441 gttcgtgatc cggccccggg tggggacgtg ggccccgagc ctcaccgccg gctcatccgc
     1501 cgacgtagat cttgacctgg ctcccggcct cgaccgtctc tcccctccgc gggttctgcc
     1561 cgacgacgcg gtcgccggac gagcccggga ggcggacgac gctcacctcg agcccgagcc
     1621 gtccgaggcg gcggaccgcg tcctcctcct gcatgccgat cacgtccggc atcgggaacc
     1681 gctggggacc cttcgacacg acgatcgtca ccgacgcgcc acgggtgacg gtcttgcccg
     1741 ccgccggctc ctggcggatc acgcggccac gcgcgacctc ggtcgagaat tcctcgagga
     1801 gccgcacgcc gaggccggcc gcctcgagca gcgcggtcgc gtcggcggtg ctgcggccgc
     1861 gcacatccgg gacctccacc ggcgggcggc cgcggctcac gacgagctcc accgtctcgc
     1921 ccgcctcgag cgttgccccg ccggccgggc tctgggagac gacgcgcccg cgctcgacct
     1981 cgtcgtggaa atcgcgcgtc acgcgaacct cgagcttcgc ctccgcgagg atctgctcgg
     2041 cgcgggcgag ccggcgcccc tcgaccttcg gcacctcgac cagctcgggc ccgcgtgaga
     2101 cgacgatggc gagcgtcgcg cctcgccggg cgttgtcacc cggcggcggg tcctgcgaga
     2161 cgatctctcc cgccggcacg tcgggcgaga agacctggcg ctcgacggtg atcgtgagcc
     2221 cgcgcgcctc cgcctcggtc gaggcggcat cgacggtcat cccggcaagg tcggggacct
     2281 cgatgtgggg gatcgcgtag gtccaggagg cccacgccga ggtcgtcagg agggcgaggg
     2341 ccgtcgccca ccgcaggacg cggcggaacc ggcgacggcg gcgcttccgc ggcgactccg
     2401 cgcgcgggat cgtgacggtg gcggcgcgat cggcctcggc ttcctcgccg ctcggcacgg
     2461 aacggacgac ctcggccagc gagggcgcgg gcggcagctc ggggaggctc gcggcgagcg
     2521 ccgcgcgcat cgagcgggcg tccggcgtgc gtcgctcgcg atccggttcg gtcgccccga
     2581 ggacggctcg gtccagtgag ggcgggacca tcgggttcgc ggccgacggc gccggcacac
     2641 gctcctggag atgcttgtac gcgatcgcca ccgaggtctc cccggtgaac ggcacccggc
     2701 cggtcagcac ctcgtacagc gcgatgcccg ccgcgtacag gtccgtgcgc tggtcggcga
     2761 cctcgccctg gatctgctcg ggtgcgaggt actggaccgt gccggtgacg gtccccggag
     2821 cggtgctgac gcgcgactcg gcgagcgcgc gcgcgagccc gaagtcggcg accttcacga
     2881 gcccgtcggt cgtgatgagg atgttctcgg gcttgacgtc gcggtggacg atcccctgcg
     2941 cgtgggcgtg ctcgagcgcg gcgagcacct gctggaggac ctcgaccgcc tgtgccggct
     3001 ggagcggccc gtgctgggac agcaccgtcc gcaggttcgg tccgcgcacg aactccatca
     3061 ccatgaagta ggtgtcgccg gcctggcccc agtcgtggac gtggacgatg ttcgggtggt
     3121 tgagcatcgc cgaggcccgg gcctcgcggc ggaagcggtc gatgaacgcg gtatcgcccg
     3181 cgagggttgc gtgcaggagc ttcagcgcga cgtcgcggtc gagcaccgga tcgcgcgcgc
     3241 ggaagacctc gcccatgccg ccggcagcga tgcgggcaac gatctgatag cggccgtcga
     3301 cggtggtctc gatcacggcg atggtaggtt gggggacacg gcctccagac cattgtaggg
     3361 ggcgcttccc cccggtacac ggaagggagc ccgcgtggcg cagcagggga gcctcgacga
     3421 ggagctggcg gcgctcgagg gccgagtggc cgccggcgag accgaccgcc gtgccctcgg
     3481 cttctcatcg ttcctccggc gggtcaagcg ggacccggag gcgcgcgagc gcttggccga
     3541 gcgggtcggc gcggtcgacc ggcgcctctt cgaggcccgc aagcgcgtga tcccgatccc
     3601 cgtgggtctc acgatcctgc tcgcgcagac gatcgtgctc gccgcggtgg ttcccgtcgc
     3661 gctcgcgttg gcgcggggca tggaccggcc gcgcccgggg ctcgccgggc tcctgctggt
     3721 gggcgccgcc gtgggtttgt cggtcagcgt gcacggcccc gcgcactgga tcgcaggacg
     3781 cacgatgggg atgcgcttcg tggggtggtt cctcggcggg ccgttccgga tccagccggg
     3841 gctgaaggtc gaccaggcga gctacctgcg ggcggatccc acctcgcgtg cggcgatgca
     3901 cgccgccgga gcgatcgcga gcaaggtcgc gccgttcgcc gtgttcggcg tcgcctacgt
     3961 ccagcacgcg cgggccggat gggagctggt gccggagtgg tcgctgtggg cgatcctcgg
     4021 cttcggcgcc ctgcagatcg tgaccgacgt cgtgtggtcg acgcggcgct ccgactggat
     4081 gcgcgtgcgc cgcgagctgc ggatcgcgcg ggcggagcag ggcgcggtgc tatcatcgag
     4141 gggagatccg gggagctcgg ggcaaccggg ctgagaggcc ggctggggac gatccgcgcc
     4201 ggcgacccgc gaacctgaac cgggtaatgc cggcgcaggg agcgcacggc cgcgcgactc
     4261 gcggccccgt ccggtgccgg cagcgacgcg aggagcgacc gtggccagca ccgacatccg
     4321 caccgacccg ctcctgatcg ccggccgctc gttctcctcc cggctgctcg tcgggaccgg
     4381 gaagttcggc tcgcacgagg cgatgcgtga cgcgctcgac gcctcgggcg cggaggtcgt
     4441 gacggtggcg ctgcggcgcg tggacctcga tgcgaccggc gggcccgaca tcctcgagtt
     4501 catcgatccg gaccggtacc tgctgctgcc gaacacctcg ggtgccgtcg acgccgacga
     4561 ggcggtgcgc ctcgcccggc tcgcgcgcgc cgccgggatg ccgccgtggg tgaagctcga
     4621 ggtcacaccc gagccgaggt acctgctccc ggacccggtc gagacgctgc gcgccgcgga
     4681 ggtcctcgtc gccgacggct tcacggtcct gccgtacatg ggggccgacc cgatcctggc
     4741 gaagcgtctc gaagaggccg ggtgcgcgac cgtcatgccg ctcgggtcgt ggatcgggtc
     4801 gaaccagggc ctgcggacgc gcgacgctgt cgccatcatc gtcgagcagg cgagcgtgcc
     4861 ggtcgtcgtc gacgccggcc tcggcgcccc gtcgcacgcc gcggcgtgca tggagctcgg
     4921 ggcagacgcc gtcctcgtga acaccgcgat cgcggtggcg agcgacccgg tggcgatggc
     4981 gcgggcgttc gcgctcgggg tcgaggccgg acggcgcgcg ttcctcgcgg gacgggcgcc
     5041 ggagctcgag gcggcggagg catcgagccc cctcacgggg ttcctcgaca aactcgcgac
     5101 gtcgcagacc gaccccgcgg tgacggaacc gtgagggtcg agcgtctcgc cgacctcgcc
     5161 gcgccgatcg gcgagcgtga tggcttcgtc gagccgtggc cgctgcggac gttcgcgcac
     5221 gcgctggcgg cgctgccgct cgacgacctc gtggccgtcg cgaacgacgc ctcgccgggc
     5281 cgtgtcgacc gcgcgctctc gaccgatgcg gccgcgcgca cgctcgagga cgtcgcggcg
     5341 ctgctatccc cggcggcggc cgagcgcctc gaggacgtgg cggcggcgtc gcgcgcgctg
     5401 acgctcgcgc gcttcggccg gacgatgcac ctgtacgcgc cgctgtacct gtcgaacgag
     5461 tgcctcacga cctgcggata ttgcgggttc gcgaagaacc tccccgtcgc gcgcaagacc
     5521 ctcggcgagg acgagacgct cgaggaggcg cgtcacctcg tccgcgaggg gtaccggtcg
     5581 ctgctgctgc tgaccggtga gcacgagcgg ctgaccgggg tcgcgttcct cgagcggcac
     5641 gtgcgggcgc tcgcgccgct cgtcccgtcg atcgccctcg aggtgcaggt gtggggcgag
     5701 gaggactacc gccgcctcgt cgctgccggg accgagggga tcgtgatcta ccaggagacg
     5761 tacgacccgc ggcgccacct cgaggttcac ctcgctgggc ggaagcgcga ctaccgctgg
     5821 cgtctgctcg ggcccgagcg cgcggcacga gccggcgcga ggcgggtggg gat
//
LOCUS       DATLGG010000195         5640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_6254, whole genome shotgun sequence.
ACCESSION   DATLGG010000195 DATLGG010000000
VERSION     DATLGG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6254"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VM638_05550"
     CDS             <1..419
                     /locus_tag="VM638_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HVM11923.1"
                     /translation="CLDLFAGTGALGIEALSRGAASCVFVERDPAAVRVIRENLERTR
                     LAPLGRVIRATVQGHLRRGSHDRPDLVFLDPPYDHPEDDLRSVLGSVDDRLVRGGRFV
                     LTRPKTGAIDVIPVNFQVERRLSYGDAVILVCREGR"
     gene            416..892
                     /gene="coaD"
                     /locus_tag="VM638_05555"
     CDS             416..892
                     /gene="coaD"
                     /locus_tag="VM638_05555"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HVM11924.1"
                     /translation="MSLVALCPGTFDPVTNGHLDIVERAAKLFDRVVVAVIENPSKEP
                     LFSVEERVTMLKEAVAPIAGVEVDSFTGLLVEYARRKGTRIVVKGIRAVSDFDYELQM
                     AQMNRELAGLETCFVPTNPNWSYLSSSLVKEVARYGGDVTPLVPEHVRARIEERLG"
     gene            892..1458
                     /locus_tag="VM638_05560"
     CDS             892..1458
                     /locus_tag="VM638_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11925.1"
                     /translation="MDIASRIRQLEELVNEAKSMPLSASILMNKDELLELVDGMKKTL
                     PDEIRQARWVVKDREELLAKARRDAEGIIEEAGRERQRLIAEQEVVVEAAREGDQILA
                     EARERARQIQLEAEDYVDGKLAGFEATMTQVRDQLADLQDQLAERVARILDVIHKGRE
                     RLRGQTVADEHLGDDEAELLAPEEEAIE"
     gene            1455..1955
                     /locus_tag="VM638_05565"
     CDS             1455..1955
                     /locus_tag="VM638_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HVM11926.1"
                     /translation="MTTVDVRDLLNRPGSSRELHVSPVLEDLGTELAAVPAETPVRVT
                     GLLESVVEGILVSGAVSGPVAFRCARCLTEFTRDVRVDVAELFGLDVEDPAEDTGYIV
                     RDGELDLEPLIRDSLVLALPFSPLCRPDCLGLCERCGGNRNLGECSCEPEVDERWSAL
                     ADLDLD"
     gene            1976..2152
                     /gene="rpmF"
                     /locus_tag="VM638_05570"
     CDS             1976..2152
                     /gene="rpmF"
                     /locus_tag="VM638_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HVM11927.1"
                     /translation="MAVPKRRQTKTRRDKRRANWKGEAPTYSECPQCKQPKLPHRVCT
                     NCGHYGGRQAVEVE"
     gene            2157..2867
                     /gene="rnc"
                     /locus_tag="VM638_05575"
     CDS             2157..2867
                     /gene="rnc"
                     /locus_tag="VM638_05575"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVM11928.1"
                     /translation="MRRRTRLEQGLGFRFRDEGLRVTSLTHRSFAFERGSGDTNERLE
                     FLGDAVLGLVVTDLAYRRFPDLPEGELAKLRAAIVNMATLADVARDLGIGEEVLLGRG
                     EARSGGAQKASILADALEAVIGALYLDRGLVAARKFIERVFWPRMLAYAQGRGPRDHK
                     TGLQELAAQSSGVVPQYRVTERGPDHAKRFTAVVLLGGEPYGSGEGTSKKEAEQQAAR
                     EAFERLTDEGRTVAAGEG"
     gene            2867..3709
                     /gene="mutM"
                     /locus_tag="VM638_05580"
     CDS             2867..3709
                     /gene="mutM"
                     /locus_tag="VM638_05580"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HVM11929.1"
                     /translation="MELPEVEVMRRDLEKDVVGRKIASVDVKRTKNTKRVLRRHKVPK
                     DFRDRLVDRKVTGADRRGKYILLGLDSEDVLVVHFGMSGQLLRGTKRTAPPQHTHVVL
                     TFATGGDLRFVDPRAFGELFVTPRDDLGKVKELAHIALDPLEHTFTWQQFSQLLAAHR
                     AKLKPLLMDQTFISGLGNIYSDEVLFAAGLRWDRRSDELSSQEVRRLYRGIQEVVHDA
                     IRLRGTTLGDQAYVDLYGKPGEFLPELKVYGRKGEPCRRCRTPLESVRVGNRSSYYCP
                     QCQS"
     gene            complement(3731..4684)
                     /locus_tag="VM638_05585"
     CDS             complement(3731..4684)
                     /locus_tag="VM638_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11930.1"
                     /translation="MPLAHVKWFVEDPTAVPLDQARLLSLPVALALLGTLLAAVVLGW
                     VERRAEMDRRLARLRRRLRPFLPFLLSLHLGVSLIAHALLGSYLAPSLRLPGGSTGTT
                     LAAIQVVIAVLIVVGLFTRAAAWLLVLSGLVGMSVYGVVPMLERAELLGIALYLGIVG
                     RRRWSVDARVAGPDEAPAMNPGAVGMLRVCAGVAIAVNGLTEKLLAPGVSEVFLTRNP
                     HFNVGAGFGVSDRAFIDLAGAVELAIGLLLISGVATRLAVLGAVVPFNVTLLFLGWLE
                     LVGHLPIYGIFLVLLVEGSGRVRDVARWARDPGATGVPSTA"
     gene            4794..>5640
                     /locus_tag="VM638_05590"
     CDS             4794..>5640
                     /locus_tag="VM638_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM11931.1"
                     /translation="MFLRSLTLRGFKSFADKTTLEFAPGISAVVGPNGSGKSNLVDAI
                     SWVLGEQGPRALRGSQMADVIFAGSANRPALGMAEVSLVIDNSAGAIPVDLTEIEVSR
                     VVYRSGESQYLIGGKPVRLMDIQELLSDTGIGRALHTVVGQGQLEDVLTARPEERRQF
                     IEEAAGIAKHRRRKERAQRKLAGLEQDLLRLQDVMTELRRQLKPLKHQAESARKHQEL
                     SDEAERLTWRLAAARLRDLQRVRDDRRHRWEEGQTRRRDAEERLRRLDADIARIGERL
                     AGTEQA"
BASE COUNT          903 a         1931 c         2060 g          746 t
ORIGIN      
        1 ggtgcctgga cctgttcgcc ggcacgggcg cgctcgggat cgaggcgctc tcccgcgggg
       61 ccgcttcatg cgtcttcgtc gagcgcgacc ccgccgcggt gcgggtgatc cgcgagaacc
      121 tcgagcggac acggctcgcg cccctcggac gggtcatccg cgccacggtc caggggcatc
      181 tgcgccgggg aagccacgat cggccggacc tcgtcttcct cgaccccccc tacgaccatc
      241 ccgaggacga tctccgctcg gtcctcggct cggtcgatga ccgacttgtc cggggcggcc
      301 ggttcgtcct cacgcggccg aagacgggtg ccatcgatgt gattcctgta aacttccagg
      361 tcgagcggcg tctctcctac ggggatgccg tgatcctcgt ctgtcgggag ggacggtgag
      421 ccttgtggct ctgtgcccgg ggaccttcga tccggtcacc aacgggcacc tggacatcgt
      481 cgagcgcgcg gccaagctgt tcgaccgcgt ggtcgtggcg gtgatcgaga acccctcgaa
      541 ggagccgctg ttcagcgtcg aggaacgggt caccatgctg aaggaggccg tggcgccgat
      601 cgccggcgtg gaggtggatt cgttcaccgg cctcctcgtg gagtacgccc ggcggaaggg
      661 gacccggatc gtcgtcaagg ggatccgggc cgtgagcgat ttcgactacg agctgcagat
      721 ggcgcagatg aaccgggagc tggcggggct ggagacgtgc ttcgtcccca cgaacccgaa
      781 ctggtcctac ctgtcgtcct cgctggtgaa ggaggtcgca cggtacggcg gcgacgtgac
      841 gccgctcgtc ccggagcacg tccgcgcccg gatcgaggag cgactcggct gatggacatc
      901 gcgtcgcgga tccggcagct cgaggagctc gtcaacgagg cgaagtcgat gccgctgtcc
      961 gcctcgatcc tgatgaacaa ggacgagctg ctcgagctcg tcgacgggat gaagaagacg
     1021 ctgccggacg agatccggca ggcgcgctgg gtcgtgaagg atcgcgagga gctgctcgcg
     1081 aaggcccgtc gggacgccga ggggatcatc gaggaggccg ggcgcgagcg gcagcggctg
     1141 atcgccgagc aggaggtggt cgtcgaagcg gcccgcgagg gcgaccagat cctcgcggag
     1201 gcgcgggagc gcgctcggca gatccagctc gaggcggagg actacgtcga cgggaagctc
     1261 gccgggttcg aggccacgat gacgcaggtc cgcgaccagc tggccgatct ccaggatcag
     1321 ctggccgagc gcgtcgcccg gatcctcgac gtgatccaca agggccgcga gcggctgcgc
     1381 gggcagaccg tcgccgacga gcacctcggg gacgacgagg ccgagctcct cgccccggag
     1441 gaggaggcga tcgaatgacg accgtcgacg tgcgcgatct cctcaaccgg cccggcagct
     1501 cgcgtgagct gcacgtgtcc ccggtcctcg aggatctcgg gaccgagctc gccgcggttc
     1561 cggcggagac gccggtgcgc gtcacgggac tgctcgagag cgtcgtcgag gggatcctcg
     1621 tcagcggcgc cgtctccggg ccggtcgcgt tccgctgtgc gcgctgcctc acggagttca
     1681 cccgcgacgt ccgcgtcgac gtcgcggagc tcttcggctt ggacgtcgag gacccggccg
     1741 aggacaccgg gtacatcgtg cgcgacggcg agctcgacct cgagccgttg atccgcgact
     1801 ccctcgtgct cgcgctaccc ttctcgccgc tctgccggcc cgattgcctc ggcctgtgcg
     1861 agcgctgcgg gggcaaccgg aacctgggtg aatgctcgtg cgaaccggag gtcgacgagc
     1921 gctggagcgc gctcgccgac ctcgacctgg actgaagacg aaaggagcga tcgacgtggc
     1981 ggttccgaag cggagacaga cgaagacccg gcgggacaag cgtcgggcga actggaaggg
     2041 cgaggcgccc acctacagcg agtgcccgca gtgcaagcag ccgaagcttc cgcaccgcgt
     2101 ctgcacgaac tgcggccact acggagggcg tcaggccgtg gaggtggagt aggcacttgc
     2161 gcaggcggac gcggctcgag cagggcctcg gcttccgctt ccgtgacgag gggctccgcg
     2221 tcacgtcgct cacccaccgc agcttcgcgt tcgagcgggg gagcggggac acgaacgagc
     2281 gtctcgagtt cctcggcgac gccgtcctgg gcctcgtggt caccgacctc gcctaccgcc
     2341 gcttccccga tctgcccgag ggcgagctcg cgaagctccg cgcggcgatc gtgaacatgg
     2401 cgaccctcgc ggacgtcgcg cgcgacctcg ggatcgggga ggaggtgctc ctgggacgag
     2461 gggaggcacg ctccggcgga gcccagaagg cgagcatcct cgccgacgcc ctggaggccg
     2521 tgatcggggc gctctacctc gaccgggggc tcgtggccgc ccggaagttc atcgagcggg
     2581 tcttctggcc gcgcatgctc gcgtacgcgc aggggcgcgg gccgcgcgac cacaagaccg
     2641 gcctgcagga gctcgccgcg cagtcgagcg gcgtcgttcc ccagtaccgc gtcacggagc
     2701 gagggccgga ccacgcgaag cggttcaccg ccgtcgtcct gctcggcggc gagccgtacg
     2761 gctccggcga ggggacgtcg aagaaagagg cagagcagca ggcggcgcgg gaggcgttcg
     2821 agagactcac cgatgaaggt cgcacggttg cggcggggga gggctgatgg agctaccgga
     2881 ggtcgaggtg atgcgccggg acctcgagaa ggacgtggtc gggcggaaga tcgcctcggt
     2941 cgacgtgaag cgcacgaaga acacgaagcg ggtcctccgc cggcacaagg tcccgaagga
     3001 tttccgggac cggctcgtcg accgcaaggt caccggcgcc gaccggcgcg ggaagtacat
     3061 cctgctcggg ctcgactccg aggacgtcct cgtcgtccac ttcgggatga gcggacagct
     3121 cctgcggggc acgaagcgca ccgcgccgcc gcagcacacg cacgtggtgc tcaccttcgc
     3181 gacgggaggg gacctccgct tcgtcgaccc gcgggccttc ggggagctgt tcgtcacgcc
     3241 gcgcgacgat ctcgggaagg tgaaggagct cgcccacatc gcgctcgacc ccctcgagca
     3301 caccttcacg tggcagcagt tctcccagct cctggcggcg caccgggcga agctcaagcc
     3361 actgctgatg gaccagacct tcatctcggg tctcggcaac atctattccg acgaggtcct
     3421 gttcgcggct gggctgcgat gggatcgccg gtcggacgag ctctcctcgc aggaggtgcg
     3481 tcgcctctac cgcgggatcc aggaggtcgt ccacgacgcg atccggctcc ggggcacgac
     3541 cctcggcgac caggcgtacg tcgatctcta cgggaagccg ggcgagttcc tccccgagct
     3601 caaggtctac gggcgcaagg gcgagccctg ccgccgctgt cgcacgcctc tcgagagcgt
     3661 ccgcgtcggc aaccgtagct cgtactactg cccgcagtgt cagagctgat cccgagggcg
     3721 cgtcggcccg tcacgcggtc gaggggacgc ccgtcgcccc ggggtcgcgg gcccaccggg
     3781 ccacgtccct gacgcgtccg gacccttcca cgagcagcac caggaagatc ccgtagatcg
     3841 ggaggtggcc gacgagctcg agccatccga ggaacagcag ggtgacgttg aacgggacga
     3901 cggcgccgag caccgccagg cgcgtggcga cccccgagat gagcaggagc ccgatcgcga
     3961 gctccaccgc tcccgccaga tcgatgaagg cacggtcgga gaccccgaag cccgcgccga
     4021 cgttgaagtg cgggttccgc gtgaggaaga cctccgagac gccgggggcg aggagcttct
     4081 cggtgagacc gttcaccgcg atcgcgacgc ccgcgcagac gcggagcatg ccgaccgcgc
     4141 ccgggttcat ggccggcgcc tcgtcgggac cggcgacgcg tgcgtcgacc gaccagcgcc
     4201 ggcgcccgac gatccccagg tagagcgcga tccccagcag ctccgcgcgc tcgagcatcg
     4261 gcacgacccc gtagacggac atccccacca gcccgctcag cacgagcagc cacgcggccg
     4321 cccgggtgaa cagcccgacg acgatcagca cggcgatcac gacctggatc gcggcgagcg
     4381 tcgtgcccgt gctcccgccg gggagccgca ggctcggggc gaggtaggag cccagcaggg
     4441 cgtgggcgat cagcgagact cccaggtgca gcgagagcag gaaaggcagg aagggccgga
     4501 gccgacgccg cagccgggcg agccgccgat ccatctccgc gcgccgctcg acccagccga
     4561 gcacgacggc cgcgagcagc gtcccgagga gggccagcgc gaccggcagc gacagcaggc
     4621 gggcctgatc gagcgggacg gccgtcgggt cctcgacgaa ccacttcacg tgtgcgaggg
     4681 gcatcgacgt ccatcatccc cgttccggga aggagcgagc aggccggatc ggctgcggag
     4741 gatccggtga gcttccgggc atccgaatca caccgctggt agcatcgccg cagatgttcc
     4801 tccgctcact cacgctccgc gggttcaagt cgttcgccga caagacgacc cttgagttcg
     4861 cccccggcat cagcgcggtg gtcggcccga acggctccgg gaagtcgaac ctcgtggacg
     4921 cgatctcctg ggtcctcggc gagcagggtc cccgggcgct ccgcggctcc cagatggccg
     4981 acgtgatctt cgcggggagc gcgaaccgtc cggctctcgg gatggccgag gtgagcctcg
     5041 tgatcgacaa cagcgcgggc gcgatccccg tcgacctcac cgagatcgag gtctcgcggg
     5101 tcgtgtaccg ctccggcgag agccagtacc tgatcggggg gaagccggtc cgcctgatgg
     5161 acatccagga actgctgtcc gacaccggga tcggccgggc gctgcacacc gtggtcggtc
     5221 agggccagct cgaggacgtt ctgaccgcgc ggcccgagga gcgccgtcag ttcatcgagg
     5281 aggccgccgg catcgcgaag caccgtcggc ggaaggagcg ggcccagcgg aagctcgccg
     5341 gcctcgagca ggacctcctc cgcctccagg acgtgatgac cgagctccgg cgccagctga
     5401 agccgctgaa gcaccaggcc gagtcggcga ggaagcacca ggagctgtcg gatgaggccg
     5461 agcggctgac gtggcggctc gccgccgccc gcctgcgcga cctccagcgc gtgcgggacg
     5521 accggcggca ccgttgggag gagggacaga cgcgccgtcg cgacgccgaa gagcgcctcc
     5581 gccggctcga cgccgacatc gcgcggatcg gggagcggct cgccgggacg gagcaggccc
//
LOCUS       DATLGG010000196         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_227989, whole genome shotgun sequence.
ACCESSION   DATLGG010000196 DATLGG010000000
VERSION     DATLGG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227989"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VM638_05595"
     CDS             <1..684
                     /locus_tag="VM638_05595"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HVM11932.1"
                     /translation="TVPERTADELALLFGGLLRHRDLSFDRNVTGVVVSSAVPQATQA
                     LREMVGRYFGFPPVVVEPGVKTGVPVLTDNPREVGADRIVNALAAFSKHGGPAIVVDF
                     GTATTFDAISERGEYLGGVIAPGLQISARALYEHTARLPRVELVPPRSVVGKNTVESV
                     QSGLVFGYASMVDGTVERIAKELGDATVVATGGLAAVVIGECRTIDHHEPWLTLDGLR
                     LVYERNAAS"
     gene            681..2186
                     /gene="lysS"
                     /locus_tag="VM638_05600"
     CDS             681..2186
                     /gene="lysS"
                     /locus_tag="VM638_05600"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVM11933.1"
                     /translation="MSDAGAPETPEERPEDVLAVRRQKLERLRERGIEPFARRYEPDA
                     RAADLQERFLDLEAGASSGERARVAGRLHGLRRLGKLWFGVLRDGSGEIQLMLDQATL
                     GAEAWALLDDLDVGDWVGAEGEVIRSRRGELSVRASELSVLAKSLRPMPEKWHGLRDV
                     ELRMRQRYLDLATNDEARSMVRRKAAMLRALRGVLDARGFLEVETPIVQTIPGGGLAR
                     PFETFHEALGIPMFLRIAPELYLKRLLVGGFDRVYEIGRNFRNEGISPQHNPEFTMLE
                     AYEAFGTYEDMMDLLEELVRAAADGVNGSLVFTFRGESVDLAQPFRRATLAEMVSEAL
                     HHHVTIDTSIMDLQKLAGRHGMDADPDWGPGKLMLEIFEKLVEPKLIEPTFVRDFPRE
                     VSPLARSHRDDPRLTEHVDLILGGVEIAPAYSELNDPDEQRSRFELQMQQKAAGAEET
                     HPYDEEFIVALEHGMPPAGGLGLGVDRLLAVLTDAPSLREVIIFPALRPEA"
     gene            complement(2169..3029)
                     /locus_tag="VM638_05605"
     CDS             complement(2169..3029)
                     /locus_tag="VM638_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11934.1"
                     /translation="MTRELVERYWRATAPPDEPTLRRLRHPGWTMEWPQSGERIVGHQ
                     NAMAIMEAYPGFPDQEIRSVQGTPDRWTVSPMMIPVRISGGGNAWFVETALEYPEQGS
                     WQGVAITELREGLVWRDIMYFVEPFEAPEWRGEITERAPDRASEPSLAEGDGNEEARR
                     ATLARYAEHVAGGDLPSAARELFHEDAVEDIPQSGERIRGMENQLAMIEHHPTRPTGG
                     VRRIFAAGNQFAWETDLDYEGERWLEVVLATFRGDKVTHAIAYFAQPFEAPEWRARWV
                     ERMDLSGFGA"
     gene            3098..>4311
                     /locus_tag="VM638_05610"
     CDS             3098..>4311
                     /locus_tag="VM638_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVM11935.1"
                     /translation="MRRVLERLRPIVIPLLVGSLGAWLALLLAGPVRYPVGPFDVELF
                     ARPGAPVTEIHLPPFGRIRADTHRAPLHLSATLTEFAAPELTSSLRSGGTHGIVRQVE
                     RDGAIAVRRHARASAAVALAGAATAGVVAFRRRWRPVAGSLLAGGVIFLLTAGTTVAT
                     YRSSAFLEPTFTGSLTLAPRLLGPVRGATDRIEDFRAELTRIVDGAVRAYGVIAAQPP
                     STSRVVALHISDVHASPVGMDVAQRLAEAFEVDLVLDSGDITTFNTPLEQEILARIPD
                     FGIPYVFVRGNHDSPATTAQIAAYPNVHVLEDETVEVLGLRVHGLPDPELTPANPDRA
                     GERERIRLLRERGEVAAERFRAAGEPPDVLLVHDERMAEPLAGVVPLVLGGHFHRTEA
                     RVLEGTPFLRVGT"
BASE COUNT          565 a         1641 c         1465 g          640 t
ORIGIN      
        1 accgtccccg agcggacggc cgacgagctc gcccttctct tcgggggcct cctccggcac
       61 cgcgacctct ccttcgaccg caacgtcacc ggcgtcgtcg tctcgagcgc ggtcccgcag
      121 gcgacccaag ccctgcgcga gatggtcggg cgctatttcg gcttcccgcc ggtggtcgtc
      181 gagccgggcg tgaagacggg cgtgcccgtc ctcaccgaca acccgcgcga ggtcggcgcc
      241 gaccgcatcg tgaacgcgct cgcggccttc tcgaagcacg gcggtccggc gatcgtcgtt
      301 gacttcggca ccgcgacgac cttcgatgcg atctccgaac gaggcgagta cctcggcggc
      361 gtgatcgcgc cgggcctgca gatctccgcg cgcgccctgt acgagcacac cgcgcgactg
      421 ccgcgcgtcg agctcgtccc gccgcgttcg gtcgtcggga agaacaccgt cgagagcgtc
      481 cagtcggggc tcgtcttcgg gtacgcctcg atggtcgacg ggaccgtcga gcggatcgcg
      541 aaggagctcg gggacgcgac ggtcgtcgcc accgggggcc tggcggcggt cgtgatcggc
      601 gagtgccgca cgatcgacca ccacgagccg tggctaaccc tcgacgggtt gcgactcgtc
      661 tacgagagga atgcggcctc gtgagcgatg ccggtgcgcc ggagacgccg gaggagcgtc
      721 cggaagacgt cctcgccgtc cgccgtcaga agctcgagcg tctgcgcgag cgcggcatcg
      781 agccgttcgc ccgccgctac gagccggacg cgcgcgccgc cgacctccag gagcggttcc
      841 tcgacctcga ggccggcgcc tccagcggcg agcgcgcgcg ggtcgccggc cggctccacg
      901 ggctccgccg cctcggcaag ctctggttcg gcgtcctgcg cgacggctcc ggcgagatcc
      961 agctgatgct cgatcaggcg acgctcgggg cggaggcctg ggcgctgctc gacgacctcg
     1021 acgtcggcga ctgggtcggt gccgaaggcg aggtgatccg ctcgcgccgg ggcgagctct
     1081 ccgtccgcgc gtccgagctt tcggtcctcg cgaagtccct ccgccccatg ccggagaagt
     1141 ggcacggcct gcgcgacgtc gagctccgca tgcgtcagcg ctacctcgac ctggcgacga
     1201 acgatgaggc gcgctcgatg gtccggcgca aggcggcgat gctgcgcgcc ctccgcggcg
     1261 tcctcgacgc ccgcggcttc ctcgaagtgg agacgccgat cgtccagacg atccccggcg
     1321 gcggcctcgc gcggcccttc gagaccttcc acgaggccct cgggatcccg atgttcctgc
     1381 ggatcgcccc cgagctgtac ctcaagcgcc tgctcgtcgg cggcttcgac cgcgtgtacg
     1441 agatcgggcg taacttccgc aacgagggga tctcgcccca gcacaacccc gagttcacga
     1501 tgctcgaggc ctacgaggcc ttcgggacct acgaggacat gatggacctg ctcgaggagc
     1561 tcgttcgcgc ggctgccgac ggcgtgaacg gctcgctcgt cttcaccttc cgcggcgagt
     1621 cggtcgacct cgcccagccg ttccgtcgcg ccacgctcgc cgagatggtc agcgaggcgc
     1681 tccaccacca cgtcacgatc gacacgtcga tcatggacct ccagaagctc gccggccgcc
     1741 acggcatgga cgccgacccc gactggggcc cgggcaagct gatgctggag atcttcgaga
     1801 agctcgtcga gcccaagctg atcgagccga ccttcgtccg cgacttccca cgcgaggtct
     1861 caccgctggc gcgctctcac cgtgacgacc ccaggctgac cgagcacgtc gacctgatcc
     1921 tcggcggggt cgagatcgcg cccgcctact ccgagctgaa cgacccggac gagcagcgca
     1981 gccgcttcga gctccagatg cagcagaagg cggccggagc cgaggagact cacccgtacg
     2041 acgaggagtt catcgtcgcc ctcgagcacg gcatgccccc cgccggcggc ctcggcctgg
     2101 gcgtcgatcg cctcctcgcc gtcctgaccg acgcccccag cctccgcgag gtcatcatct
     2161 tccccgccct acgccccgaa gcctgagagg tccatccgct cgacccagcg cgcccgccac
     2221 tcgggggctt cgaacggttg ggcgaagtag gcgatcgcgt gcgtcacctt gtcgccgcgg
     2281 aaggtggcca ggacgacctc gagccagcgt tcgccctcgt agtcgaggtc ggtctcccag
     2341 gcgaactggt tgcccgcggc gaagatgcgg cgaaccccgc cggtcgggcg ggtgggatgg
     2401 tgctcgatca tcgccagctg gttctccatg ccgcggatcc tctcccccga ctgcgggatg
     2461 tcctcgacgg cgtcttcgtg gaagagctcc cgggcggccg agggcaggtc cccgccggcg
     2521 acgtgctccg cgtagcgcgc cagcgtcgcg cgtcgggcct cctcgttccc gtcgccctcc
     2581 gccaacgacg gttcgctcgc gcgatccggc gcgcgctcgg tgatctcccc gcgccactcg
     2641 ggcgcctcga acggctcgac gaagtacatg atgtcgcgcc atacgagccc ctcacgcagc
     2701 tcggtgatcg ccaccccttg ccagcttccc tgctccggat actcgagcgc cgtctcgacg
     2761 aaccacgcgt tgccgccgcc ggagatccgg acggggatca tcatcggcga gacggtccag
     2821 cggtccgggg tcccctggac cgagcggatc tcttggtccg ggaagcctgg ataggcctcc
     2881 atgatggcca tcgcgttctg gtgcccgacg atccgctctc ccgactgcgg ccactccatc
     2941 gtccagccgg ggtgccggag gcgccgaagc gtcggctcgt ccggcggagc cgtcgccctc
     3001 cagtagcgct ccacgagctc gcgggtcatc gttcctccct tagccggagc aagcctacag
     3061 gcggcgagcc gatcgctgca ctcgctaggg ttggagggtg cgcagggtgc tcgagcgcct
     3121 ccggcccatc gtcatcccgc tcctcgtggg ctcgctgggc gcctggctgg ccctcctgct
     3181 cgcgggcccc gtccgctatc ccgtggggcc gttcgacgtc gagctcttcg cgcggcccgg
     3241 ggcgcccgtc acggagatcc acctgccgcc gttcggccgg atccgcgccg acacgcaccg
     3301 cgcgccgctg cacctgagcg caacgctgac cgagttcgcc gcgccggagc tcacgtcgtc
     3361 gctccgatcc ggcgggacgc acgggatcgt ccggcaggtc gagcgcgacg gcgccatcgc
     3421 cgtccggcgg cacgcccgcg cgtcggccgc ggtcgccctt gcgggagcgg cgaccgccgg
     3481 cgtcgtcgcg ttccgccgcc gctggcgccc ggtcgccgga tcgctcctcg ccgggggcgt
     3541 catcttcctc ctcacggccg ggacgaccgt cgccacctat cgttcgagcg cgttcctcga
     3601 gccgacgttc acggggtcgc tcacgctcgc gccgcggctg ctcggcccgg tccgcggcgc
     3661 gaccgaccgc atcgaggact tccgcgcgga gctcacgcgg atcgtcgacg gagccgtccg
     3721 tgcctacggt gtgatcgcgg cccagcctcc ctcgacgagc cgcgtcgtcg cgctgcacat
     3781 cagcgacgtc cacgcctcac cggtcgggat ggacgtggcc cagcgcctgg cggaggcgtt
     3841 cgaggtcgac ctcgtcctcg acagcggcga catcacgacg ttcaacacgc cgctcgagca
     3901 ggagatcctg gcccggatcc ccgacttcgg catcccgtac gtcttcgtcc gcgggaacca
     3961 cgactccccg gcgacgaccg cgcagatcgc cgcctacccg aacgtccacg tcctcgagga
     4021 cgagacggtc gaggtcctgg gcctgcgcgt ccacggcctc cccgacccgg agctgacccc
     4081 ggcgaacccc gaccgggcgg gggagcgcga gcggatccgg ctcctgcgcg agcgtggtga
     4141 ggtcgcggcc gagcgcttcc gcgccgcggg cgagccgccg gacgtcctgc tggtgcacga
     4201 cgagcggatg gcggagcccc tcgcgggcgt cgtgccgctc gtgctcggcg gtcatttcca
     4261 ccgcaccgag gcccgggtcc ttgaggggac gcccttcctg cgggtgggga c
//
LOCUS       DATLGG010000197         4792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_228007, whole genome shotgun sequence.
ACCESSION   DATLGG010000197 DATLGG010000000
VERSION     DATLGG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228007"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VM638_05615"
     CDS             complement(<1..265)
                     /locus_tag="VM638_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthetase"
                     /protein_id="HVM11936.1"
                     /translation="MTAVVAICGPGNARPAEVEAAAFVARRLAEAGATIVCGGLGGVM
                     DAAARGAREAGGTVVGILPGPTREGASPHLSVALPTGMGEARNA"
     gene            complement(262..2511)
                     /locus_tag="VM638_05620"
     CDS             complement(262..2511)
                     /locus_tag="VM638_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVM11937.1"
                     /translation="MDADGFLRSLEHDLEVDERLVHVERIAAREPDPQPFPDLPDPLP
                     ARLRHLGLDGLHPHQATALDHLTAGRNVIVATGTASGKTLVYNLELARRVLTEPHATG
                     LYLFPTKALARDQLRQIRELRLPQLRPAVYDGDTPKQERPLIRRTANLILTNPDMLHA
                     SILPNHARWADFLLRLALVVVDEAHVARGVFGSHVAMILRRLRRLVARYGGDPRFVLA
                     SATVGNPGELAERLVGVPFEEVREDASPRGEKVFCLWNPPMIDEEEGTRKGAVGEAGF
                     LLSRLVGSGARTIAFGRTRRSAELLADIAKRQTDDVLAERIKAYRAGYLPEERRALER
                     DLAEGRLLGVAATNALELGIDIGSLDAAVLAGYPGTRASLWQQAGRAGRRGSGSIAIL
                     VAQDDPLDQYLVTHPDDLFRKPPEAAVVDPTNPYVVGPHLRCAAQEHPLDPEELEVFG
                     DAAPDVVASLLETGGLAERRGKLHNRGEAPHRAVDIRSASSRVVLIVEEATGSLLGTV
                     DEARSHSQVHEGAIYLHQGEQYEVRSLDLGEGVALVHRSSTDVYTQAKRTTDIDVLRV
                     LAESKVAETPMFFGEVRVTTQVTGYQRKLPSGEILDTVLLDLPPQTLVTRAVWWAIPD
                     ATIVRAGVPPASLPGAAHAAEHAAIGLLPLVATCDRWDVGGVSTAYHPDTDSCAIFIY
                     DGYPGGAGISERGFVQGERLLRATLEAIRACPCRDGCPSCVQSPKCGNGNEPLDKHRA
                     ADLLERILG"
     gene            complement(2613..3410)
                     /locus_tag="VM638_05625"
     CDS             complement(2613..3410)
                     /locus_tag="VM638_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11938.1"
                     /translation="MRSATLAAVLALAVAAVAPSAGADSEATTVITDAAGDANFVNGN
                     GLSSLAPTDGVDTRPASIAGADLRSVDFSTPYDTIKVYNQDGTVSQVLYSPKAFRVRL
                     QMEGDVMPTFGPTIIVRILTNIAGCDVWFQAIIRGSNPDAAADPHRADIRLPAAASCP
                     GNAAVLSIANGFTLDVSGKTVTMTYPFEAFTGNHAGWIKPGTQIDPDGSFLNNANTPH
                     VRPWLTSLGVTVPSIDETARPQAFVVGSDVPQTLVCSQQPAHPDCQP"
     gene            complement(3528..4439)
                     /locus_tag="VM638_05630"
     CDS             complement(3528..4439)
                     /locus_tag="VM638_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HVM11939.1"
                     /translation="MRRLSVLVAGAVVALIGVNVVTGVRMEPVRAQTPAVEIQRVQDA
                     AASPLVASKEPIFILALGSDARPHEPVNRLHSDAIQLIGINPAKGGAGILGFPRDSWV
                     TLPGRGQTRINDAMVYGGPQLAVETVESITGIKIDYYALTSFPEFQRMVKGIGGVTIE
                     IEYPMHDDASGTSFDPGRRRLGGADALAFARDRKSAPGGDLGRSDNQGRLLIAALKKL
                     RKDVERNPATLLRWLRTGVENVETDLKLGEIFDLLAGATQIDPSKVRNAVVPATDGEV
                     NGASVVFISDGAQALYDDLADDGMLAR"
     gene            complement(4436..>4792)
                     /locus_tag="VM638_05635"
     CDS             complement(4436..>4792)
                     /locus_tag="VM638_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11940.1"
                     /translation="FPKVVEAFAPGTVDAAQRDLAELTLGPGSARITRVVPAESRLDV
                     GILLSKKEPVAAVARTAFGADATVRGGGSVRVSHGGTYFLRMIDGTWKIVGYDVDGSI
                     ESVDPQAGPTPTGSAG"
BASE COUNT          647 a         1678 c         1747 g          720 t
ORIGIN      
        1 gggcgttgcg cgcctcgccc atccccgtgg gcagcgccac gctcaggtgc ggagaggcac
       61 cctcccgggt cgggcccggc aggatgccga cgacggtccc gccggcttcg cgcgcgccgc
      121 gcgcggcggc gtccatcact cccccgaggc cgccgcacac gatcgtcgcc ccggcctccg
      181 ccagccgccg tgcgacgaac gccgcggcct ccacctcggc tgggcgcgcg ttcccgggcc
      241 cgcagatcgc cacgaccgcc gtcatccgag gatccgctcg aggaggtcgg cggcgcggtg
      301 cttgtccagg ggctcgttcc cgttgccgca cttcggcgac tggacgcacg aggggcagcc
      361 gtcgcggcag gggcacgcgc ggatcgcctc gagggtcgca cgcaggagcc gctcgccctg
      421 gacgaacccg cgctcgctga tccccgcccc gccgggatag ccgtcgtaga tgaagatcgc
      481 gcaggaatcg gtgtcggggt ggtaggcggt cgagacgccg ccgacgtccc agcggtcgca
      541 ggtggcgacg agcggcagga gcccgatcgc cgcgtgctcc gcggcgtggg ccgcgcccgg
      601 gaggctcgcc ggtggcacgc cggctcggac gatcgtcgcg tcggggatcg cccaccacac
      661 cgcgcgcgtg acgagggtct gcggcgggag gtcgagcagg accgtgtcga ggatctcgcc
      721 ggacggaagc ttgcgctgat acccggtgac ctgtgtcgtg acgcgcacct cgccgaagaa
      781 catcggcgtc tccgcgacct tcgactcggc gaggacccga agcacgtcga tgtccgtcgt
      841 ccgcttcgcc tgcgtgtaga cgtcggtgct ggaacggtgg acgagcgcca cgccttctcc
      901 aaggtcgagg gagcgaacct cgtactgctc gccctgatgg aggtagatcg cgccctcgtg
      961 gacctgcgag tgggagcgcg cctcgtcgac ggtcccgagg agcgagcccg tcgcctcctc
     1021 gacgatcagc acgacacgcg acgaggcgga ccggatgtcg acggcccggt gcggggcctc
     1081 gccacggttg tggagcttcc cgcgtcgctc ggcgagccca ccggtctcca ggagcgacgc
     1141 gaccacgtcc ggtgcggcgt cgccgaacac ctcgagctcc tccgggtcca gggggtgctc
     1201 ctgcgcggcg cagcggagat ggggccccac cacgtacggg ttcgtcggat cgacgacggc
     1261 ggcctccggc ggcttgcgga agaggtcgtc cgggtgggtc accaggtact ggtcgagcgg
     1321 gtcatcctgc gcgacgagga tcgcgatcga gccgctgccc cgccgtccgg cgcgccccgc
     1381 ttgctgccag agcgacgcgc gggttcccgg gtagccggcg aggaccgcgg cgtcgagcga
     1441 cccgatgtcg atcccgagct ccagcgcgtt cgtcgcggcc acgccgagca gcctcccctc
     1501 ggcgaggtcc cgttcgagcg cgcgtcgctc ctcggggagg tacccggcgc ggtaggcctt
     1561 gatccgttcg gccaggacat cgtccgtctg gcgcttcgcg atgtcggcga gcagctcggc
     1621 gctccgccgc gtccgtccga acgcgatcgt ccgcgcaccg cttccgacga gccgtgacag
     1681 gaggaagccg gcctcgccga cggctccctt ccgcgttccc tcctcctcgt cgatcatggg
     1741 cgggttccac aggcagaaga ccttctcgcc gcgcggcgag gcgtcctcgc gcacctcctc
     1801 gaacggcacg cccacgagcc gctcggcgag ctcgcccggg ttgccgacgg tcgcagaggc
     1861 gaggacgaac cgcgggtcgc cgccgtagcg cgcgacgagg cgccgcaggc ggcgcaggat
     1921 catggccacg tgggagccga agacgccgcg cgccacgtgc gcctcgtcga cgacgacgag
     1981 cgcgagccgc aggaggaagt ccgcccagcg cgcgtggttc ggaaggatcg acgcgtggag
     2041 catgtcgggg ttcgtgagga tcaggttcgc cgtccggcgg atgagcgggc gctcctgctt
     2101 cggcgtgtca ccgtcgtaga ccgccgggcg gagctggggc aggcggagct cacggatctg
     2161 ccggagctgg tcgcgcgcga gcgctttcgt cgggaagagg tagagaccgg tcgcgtgcgg
     2221 ctccgtgagc acgcgccggg cgagctcgag gttgtagacg agggtcttgc cgctcgccgt
     2281 ccccgtcgcc acgatgacgt tgcgccccgc ggtcaggtgg tcgagcgccg tggcctgatg
     2341 cgggtggagg ccgtcgagtc cgaggtgacg gaggcgcgcc ggcagcgggt cggggaggtc
     2401 ggggaagggc tgcgggtccg gttccctcgc cgcgatccgc tcgacgtgga cgagccgctc
     2461 gtcgacctcg aggtcatgct cgagcgaccg caggaacccg tccgcgtcca taccgccgat
     2521 gttctcaggt cacggggccc cgtgcggccc gccgccgacc cggacgcgac gagggcccgg
     2581 ccggagccgg gccctcgctt cgtgcgtgtt cgctacggct ggcagtccgg gtgcgcgggc
     2641 tgctgcgagc agacgagcgt ctgtggaacg tcagacccga ccacgaacgc ctggggccgc
     2701 gccgtctcgt cgatcgaggg gacggtcacg ccgagagacg tgagccacgg gcgcacgtgc
     2761 ggggtgttcg cgttgttgag gaacgatccg tcgggatcga tctgcgtccc gggcttgatc
     2821 catcccgcgt ggttcccggt gaacgcctcg aacgggtagg tcatcgtcac ggtcttcccg
     2881 ctgacgtcga gcgtgaagcc gttcgcgatc gagaggacag ccgcgttgcc cgggcagctc
     2941 gcggccgcag ggaggcggat gtcggctcgg tgcgggtcgg cggcggcgtc ggggttggat
     3001 ccgcggatga tggcctggaa ccagacgtca catcccgcga tgttcgtcag gatcctgacg
     3061 atgatcgtcg ggccgaaggt cggcatgacg tcgccctcca tctgcaggcg cacgcggaac
     3121 gccttcgggg agtagaggac ctggctcacg gtcccgtcct ggttgtagac cttgatcgtg
     3181 tcgtacggcg tcgagaagtc caccgaccgc aggtcggcgc cggcgatcga tgccggacgc
     3241 gtgtcgaccc cgtcggtcgg ggcgaggctc gacagcccat tgccattgac gaagtttgca
     3301 tctccggccg cgtcagtgat gactgtcgtc gcctctgagt cggcacccgc ggatggtgct
     3361 acagcggcca ccgccagcgc gagcacggcg gcgagggtgg cgctacgcat aggatcctcc
     3421 tcctgtcgat tccgtcgctg tcgtgttcgg cttcgggagc caggatgcct ttgtgcaatt
     3481 gcacagagtt ggcggaggcg ccgccgcggg atcgggaggg atgggaccta tcgggcgagc
     3541 atgccgtcgt cggcgaggtc gtcgtagagc gcctgagcgc cgtcggagat gaacacgacg
     3601 ctggcgccgt tgacctcgcc gtcggttgcg gggacgaccg cgttgcggac cttcgacgga
     3661 tcgatctgcg tcgctccggc cagcaggtcg aagatctccc cgagcttgag gtcggtctcg
     3721 acgttctcga cgccggttcg gagccatctc aggagcgtcg ccgggttccg ctcgacgtcc
     3781 ttccgcagct tcttcagcgc ggcgatcagc agccgtccct ggttgtcgga gcggccgagg
     3841 tccccaccgg gcgcgctctt gcggtcgcgc gcgaaggcga gggcgtcggc gcctccgagc
     3901 ctccgccggc cggggtcgaa gctcgtcccc gatgcgtcgt cgtgcatcgg gtactcgatc
     3961 tcgatcgtca ccccgccgat gcccttcacc atccgctgga actcggggaa ggacgtgagg
     4021 gcgtagtaat cgatcttgat cccggtgatg ctctccaccg tctcgaccgc gagctgcggt
     4081 cccccgtaga ccatcgcgtc gttgatccgc gtctggccgc gacccggaag cgtgacccac
     4141 gagtcacgcg ggaacccgag gatccccgcc ccgcccttcg ccgggttgat gccgatcagc
     4201 tggatcgcat cggagtggag ccggttcacg ggctcgtgcg gccgcgcgtc gctcccgagc
     4261 gcgaggatga agatgggctc cttgctggcc accagcgggg aagccgcggc gtcctgcacg
     4321 cgctggatct cgacggccgg cgtctgcgcc cgcaccggct ccatgcggac accggtgacg
     4381 acgttcacac cgatgagcgc gacgaccgcg ccggcgacga ggacggagag gcgcctcatc
     4441 ccgcgctccc ggtcggcgtc ggaccggcct gcggatccac cgactcgatc gagccgtcga
     4501 cgtcgtagcc cacgatcttc cacgtgccgt cgatcatccg caggaaatac gtgccgccgt
     4561 gactcacgcg gacgctccct ccgccgcgga ccgtcgcgtc cgcgccgaag gccgttcgcg
     4621 cgaccgcggc gacgggctcc ttcttcgaga gcaagatccc gacgtcgaga cggctctccg
     4681 ccggcaccac ccgggtgatc cgcgcggatc ccggccccag cgtcagctca gccaggtcgc
     4741 gctgagcggc gtcaaccgtc cccggtgcga acgcctccac gaccttgggg aa
//
LOCUS       DATLGG010000198         3244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_169758, whole genome shotgun sequence.
ACCESSION   DATLGG010000198 DATLGG010000000
VERSION     DATLGG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169758"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VM638_05640"
     CDS             complement(<1..783)
                     /locus_tag="VM638_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVM11941.1"
                     /translation="MKKRFVWMAALSALVLITAACRQQGGQQGDNGTTDGDPCGGEFG
                     CVEYGPNDPIKLGTLLVISGSDASLGQDSQNGAVLALDFRDTPGEVLGHPVEFNHQDD
                     GCSAEGGQAGAQALASDPTIAAVIGTSCSSAALGVADTILSEQGIVLISPSNTGPALT
                     APETHQPFYLRTAHNDKIQGGAVADFAYNEAGKRKAATIHDGSPYADGLQQVFADNFT
                     RLGGEITAQQAIQVGETDFGPVLTDIAADQPDFLYFPVFVAEG"
     gene            complement(983..1270)
                     /locus_tag="VM638_05645"
     CDS             complement(983..1270)
                     /locus_tag="VM638_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11942.1"
                     /translation="MHGIEARTAGVDRQMARALARLSRLRVEAAEASDALADARLRML
                     IAETPVADEAVVAGAARLRRVREALRAAESALACLQRDRLDGGETAAPVRP"
     gene            1335..1417
                     /locus_tag="VM638_05650"
     tRNA            1335..1417
                     /locus_tag="VM638_05650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1368..1370,aa:Leu,seq:caa)
     gene            complement(1433..>3244)
                     /gene="dnaE"
                     /locus_tag="VM638_05655"
     CDS             complement(1433..>3244)
                     /gene="dnaE"
                     /locus_tag="VM638_05655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVM11943.1"
                     /translation="EELGLLKMDFLGLRNLSVIEDAVRMLRERGVTLDIDHVPLDDEA
                     TYAMLRRAETTGVFQLESPGMRSLIRELAPDRFEDLMALVALYRPGPLNAGMHIEYAE
                     RKHGRRPVTYPHPALETILAGTYGVIVYQEQVMEIAVRMSGYEMGEADLLRKAMGKKI
                     REELTPHRTKFVQGAVERGCEQRVAEHIFDDLIMPFADYGFNASHACGYGFVAYQTAY
                     LKAHHPVEYFAALLTSVKDDKDKKPYYLNACRTMGITVLPPDVNASDVDFTPHGGDIR
                     YGLSAVRNVGEGAVGQIIRARREKGAFTSFADFCRKVDVSVLHKRVLESLILAGAFDS
                     LGYGRRGLLETYDKVVGPIVSERRAEEAGQFSLLGAENGQPAGSEIDESVLSREEFEK
                     ATLLRHEKEMLGSYVTDHPLLAVRERLEAQTDREMADLASLGDGEVVTLGGIIVGVQR
                     RYTKRGEPYALLRLEDLVGGVQVVCFPSVYERAGDLVAPDRIILVKGRADLRGRELQI
                     VAMELRELDGTAPPRAARSEPLLLEVPAVQCTGGLVVRLKEVLAGHPGQVPVVLRLTS
                     NGSGTTLRLGDGYRVDRSGGLLAELRSLLGPESVTTG"
BASE COUNT          468 a         1161 c         1116 g          499 t
ORIGIN      
        1 accctcagcg acgaagaccg ggaagtacag gaagtccggc tggtccgccg cgatgtccgt
       61 gaggaccgga ccgaagtccg tctcaccgac ctggatcgcc tgctgggccg tgatctctcc
      121 accgagcctg gtgaagttgt ccgcgaacac ctgctggaga ccgtcggcgt aggggctgcc
      181 gtcgtggatc gtggcggcct tccgcttgcc ggcctcgttg taggcgaagt cggccaccgc
      241 gccaccctgg atcttgtcgt tgtgcgcggt ccgcaggtag aagggctggt gcgtctccgg
      301 cgccgtcagc gccgggcccg tgttcgaggg cgagatcagc acgatgccct gctcggacag
      361 gatcgtgtcc gcgacgccga gcgccgcgct cgagcagctg gtgccgatga ccgcggcgat
      421 cgtcgggtcg gacgcgagcg cctgggcacc ggcctgtccg ccttcggccg agcagccgtc
      481 gtcctggtga ttgaactcca ccgggtgccc gaggacctcg ccgggcgtgt cacggaagtc
      541 gagcgcgagc accgcgccgt tctggctgtc ctggcccaga gaagcgtcgg aacccgagat
      601 cacgagcagc gtcccgagct tgatcgggtc gttcggtccg tactccacgc agccgaactc
      661 gccgccgcac gggtcgccgt cggtcgtacc gttatcaccc tgctggccac cctgctgccg
      721 gcaggcggcg gtgatgagca cgagcgcgct cagcgccgcc atccaaacga atcgcttctt
      781 catcaagtcc tccttgtgcg ggcgaccttc cgaaaccttg ccgaaagaag tgcctagcac
      841 gacccccttt ccctctctcg cggtgtcctg cctcccaaca ggtcgagcat atttaccaca
      901 gtcgtccggc ggtgtccaag cgcgcccccc gtcgcccgtt cgggggtccg ccggacgtcg
      961 ggcctgcccg tccatacggg ttctacggac ggacgggcgc tgcggtttcc cctccgtcga
     1021 ggcgatcgcg ctgaaggcac gccagcgccg actcggccgc gcgcagcgcc tcgcggaccc
     1081 tgcggagccg cgccgcgccg gccacgacgg cctcgtccgc gacgggggtc tcggcgatca
     1141 gcatccgcag gcgggcgtcg gcaagggcgt ccgacgcctc ggccgcctcg acgcgcagcc
     1201 gcgagaggcg cgccagagcc ctggccatct gccggtcgac gccggccgtc cgagcctcga
     1261 tgccgtgcat ggggaaggga agggcagtgt aggagccgga caccccttcg gcaagaccta
     1321 tactcgccgt cgaggccggg gtggtggaac ggcagacacg ggggcctcaa aagcccttgg
     1381 gcgcgagccc gtgagggttc gaatccctcc cccggcaccc cgcccgaggg gatcagccgg
     1441 tggtcaccga ctcgggaccg agcagcgacc gcagctccgc caacaggccc cccgagcggt
     1501 ccacccggta gccgtcgccg aggcggagcg tggtccccga cccgttcgat gtcaggcgca
     1561 ggaccacggg cacctgcccg gggtgcccgg cgaggacctc cttcagccgg acgacgagcc
     1621 cgccggtgca ttggaccgcc ggcacctcga ggagcagcgg ctcgctccgc gcggcgcggg
     1681 gtggagcggt cccgtccagc tcccgcagct ccatcgcgac gatctggagc tcccgccccc
     1741 ggaggtccgc gcggcccttc acgaggatga tgcggtccgg ggcgaccagg tcccccgcgc
     1801 gctcgtacac cgaggggaag cacacgacct ggacgccgcc gacgagatcc tcgaggcgga
     1861 gcaacgcgta cggctcgccg cgcttcgtgt accgacgctg gaccccgacg atgatcccgc
     1921 cgagcgtcac gacctctccg tcgccgaggg aggcgaggtc cgccatctcg cggtccgtct
     1981 gcgcctcgag gcgctcgcgg accgcgagca gcgggtggtc cgtgacgtag gatccgagca
     2041 tctccttctc gtggcgcagg agcgtcgcct tctcgaactc ctcgcgcgag aggaccgact
     2101 cgtcgatctc gcttccggcg ggctgcccgt tctcggcccc caggagcgag aactgccccg
     2161 cctcctcggc ccggcgctcc gacacgatcg gtccgaccac cttgtcgtag gtctcgagga
     2221 gaccgcgccg cccgtacccg agcgagtcga aggcgccggc gaggatgagg ctttcgagca
     2281 cgcgcttgtg caggacgctg acgtcgacct tgcggcagaa gtcggcgaag gacgtgaagg
     2341 cgcccttctc gcgtcgcgcc cggatgatct ggccgacggc gccctccccc acgttgcgaa
     2401 cggccgagag cccgtagcgg atgtccccgc cgtgcggcgt gaagtccacg tcggaggcgt
     2461 tgacgtccgg cggcaggacc gtgatgccca tcgtccggca cgcgttgagg tagtagggct
     2521 tcttgtcctt gtcgtccttc acgctcgtca gcagcgccgc gaagtactcg acggggtgat
     2581 gggccttcag gtacgccgtc tggtaggcga cgaacccgta gccgcacgcg tgcgaagcgt
     2641 tgaagccgta gtcggcgaag ggcatgatca ggtcgtcgaa gatgtgctcg gcgacccgct
     2701 gctcgcatcc gcgctcgacc gctccctgca cgaacttcgt ccggtgcggc gtcagctctt
     2761 cccggatctt cttgcccatc gccttgcgga gcaggtcggc ctcgcccatc tcgtagccgg
     2821 acatgcgaac ggcgatctcc atgacctgct cctggtacac gatcaccccg taggtgccgg
     2881 cgaggatcgt ctccagggcc gggtgcggat aggtcacggg tcggcggccg tgcttgcgct
     2941 ccgcgtactc gatgtgcatc ccggcgttga gggggcccgg gcggtacagg gccaccagcg
     3001 ccatgaggtc ctcgaaccgg tcgggcgcca gctcgcggat cagcgagcgc atcccggggc
     3061 tctcgagctg gaagacgccg gtggtctcgg cccgccggag catcgcgtac gtggcctcgt
     3121 cgtcgagggg cacgtggtcg atgtcgagcg tcaccccgcg ctcgcgaagc atccggaccg
     3181 cgtcctcgat caccgagagg ttgcggagac cgaggaagtc catcttcagc aggccgagct
     3241 cctc
//
LOCUS       DATLGG010000199         5384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_228240, whole genome shotgun sequence.
ACCESSION   DATLGG010000199 DATLGG010000000
VERSION     DATLGG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228240"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VM638_05660"
     CDS             <1..587
                     /locus_tag="VM638_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HVM11944.1"
                     /translation="AIEANISCPNVERRNQVFACRPELAAEVVGAVSRRARLPVFAKL
                     TPDVTDIAEVARACVEAGAHGLSLINTLLGMAIDVDTFRPRLGGVTGGLSGPAIRPVA
                     VRAVYQVAQALPGVPLIGMGGVRTWRDAVELMLAGAWAVAIGTANFFDPHVTIEVAQG
                     VGRFLADRGLTSPADLRGKVKVHGETLEPAHRGG"
     gene            550..1230
                     /gene="pyrF"
                     /locus_tag="VM638_05665"
     CDS             550..1230
                     /gene="pyrF"
                     /locus_tag="VM638_05665"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVM11945.1"
                     /translation="MARPSNPLIAAVDLSDLAEAERLGGALAGTVGHLKVGLELFTAA
                     GPPAVERLGRHAPLFLDLKLHDIPTTVERAARNVGRLGTAMLTVHALGGPAMVAAAVA
                     GAERGAADAGVEPPAIVAVTVLSSLAGEGLALPSSLAYEAVDAGARGVVVSGDDVRQV
                     REVLGSGPLLIVPGIRPSAHPTNDHARVLSPGEALEAGADHLVIGRPITGAPDPAEAA
                     RAILREIT"
     gene            1227..1790
                     /gene="pyrE"
                     /locus_tag="VM638_05670"
     CDS             1227..1790
                     /gene="pyrE"
                     /locus_tag="VM638_05670"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVM11946.1"
                     /translation="MNERETLDLLREKGAVLDGHFLLTSGRHSPTYVEKARIFEHPAA
                     VERLAAEVAAWHGGVEAVVSPAVGAIPFGFAVALAAGSRFLYAERQDGAMTLRRGFSL
                     RAGERVLVVEDVVTTGGSAAEVAALVRDSGAELLGVAALVDRSDGTTGLPLRALVRIE
                     AQTYAPEECPLCRDGRPLESHGSRHLA"
     gene            1851..2165
                     /gene="mihF"
                     /locus_tag="VM638_05675"
     CDS             1851..2165
                     /gene="mihF"
                     /locus_tag="VM638_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HVM11947.1"
                     /translation="MPLPTLTPEQRREALAKAAEARKKRAEVKDRLKSGSMSLKDVLG
                     KQSDSVVGKMKVSTVLESLPGVGKVRARRIMERLDISSSRRVRGLGTKQREALAAEFA
                     NK"
     gene            2173..2754
                     /gene="gmk"
                     /locus_tag="VM638_05680"
     CDS             2173..2754
                     /gene="gmk"
                     /locus_tag="VM638_05680"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVM11948.1"
                     /translation="MAARGRLFVISGPSGVGKGTVVRRLIDLRPDLAFSVSYTTRRRR
                     PTEREGRHYRFVDREAFDRLIEDGQFLEWAEVFGERYGTPSAEVEVARRAGRDVLLEV
                     NMEGARSVRARVPDAVLVFLAPPSEEELARRLSARGTESSQELQRRLREARREMAEAP
                     AFDHVVLNDEVERAAAAILAIIDATPPAAEGDV"
     gene            2754..3008
                     /gene="rpoZ"
                     /locus_tag="VM638_05685"
     CDS             2754..3008
                     /gene="rpoZ"
                     /locus_tag="VM638_05685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HVM11949.1"
                     /translation="MIEPSIDQLMDRVDSKYTLVILAAKRAREINSYYSQLGEGRGEF
                     VPPLVETGGLRNKPLSIAMEEIADDKVTFERSEPAPDDEE"
     gene            3012..4208
                     /gene="coaBC"
                     /locus_tag="VM638_05690"
     CDS             3012..4208
                     /gene="coaBC"
                     /locus_tag="VM638_05690"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HVM11950.1"
                     /translation="MEGPLAGRTVLLGVTGGIAAYKAALLARLLREDGAEVQVVMTPS
                     ATRFIGPDTFAALTGRSVHSDVFERPEAVLHVRLAREADLAVVAPATANLLARLALGL
                     ADDLLSSTLLELRAPVVVAPAMHTGMWSSAATRTNLATLRDRGVRIVGPVEGALAAGD
                     RGMGRMAEPDEILAEVRAALAGPGDLAGRRLLVTAGPTHEPIDPVRFIGNRSSGKMGY
                     AVAAAAARRGAAVTLVSGPTRLDPPPGVEVVRVETAEEMRSAVLARLDDSDAVVKAAA
                     VADFRPARSATRKLKKEAGVPDLGLEPTPDILREVGERAGERVLVGFAAETEDLETAG
                     RRKLAEKGLDLVVVNEVGSPGTGFDADTNRAMILSAGGDDEPLRDWTKVELAEAICDR
                     IAKRLA"
     gene            4272..>5384
                     /locus_tag="VM638_05695"
     CDS             4272..>5384
                     /locus_tag="VM638_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11951.1"
                     /translation="MKLAFRSLLPLALVFGIPGVPATAGGGLLPDGPGLESEGDIVHV
                     ANVSYGGGSDLEFATKTVRVLDENGEPVCARDERGECVRDANGDVVYETEERDYAFAG
                     RLSQPVQVIDITDPAKPVRVAEVPCRFNQNDVQVFGDTLLMAADASGPCTMANGATTN
                     GAGFATADISDPRFPRVIGRANQPGGAHTLTVHPSRPLVYVSNGDISVNGSIHIWSIA
                     NPANPVKVRDWAPQGAVEPPHDITFNATGTRAYSAAQDAHTFILDTTDPQNPSTISVI
                     VNEGISYSHQADPTPDGKYLLIGDELGGGAATLPSPGGPVHVYDIRNEQRPVKVGVIG
                     TDATGVNGVSTAHVFRINPDGWTMAIGWYRDGVHVWD"
BASE COUNT          747 a         1966 c         1954 g          717 t
ORIGIN      
        1 cggcgatcga agcgaacatc tcctgcccga acgtcgagcg ccgcaaccag gtgttcgcct
       61 gccgccccga gctcgccgcc gaggtggtcg gcgcggtcag ccgccgggcg cggctgccgg
      121 tgttcgcgaa gctcacgccg gacgtcacgg acatcgccga ggtcgctcgc gcgtgcgtcg
      181 aagcgggagc gcacggcctc tcgttgatca acacccttct cgggatggcg atcgacgtcg
      241 acacgttccg cccgcggctc ggcggggtga ccgggggcct gtcggggccg gcgatccgcc
      301 cggtcgccgt ccgcgcggtc taccaggtgg cgcaggcgct gcccggggtc ccgctgatcg
      361 ggatgggcgg ggtgcggacg tggcgtgacg cggtcgagct gatgctcgcc ggcgcgtggg
      421 cggtcgcgat cggcaccgcg aacttcttcg acccgcacgt cacgatcgag gtcgcgcagg
      481 gcgtcgggcg cttcctggcg gatcgtggcc tgacgagccc cgcggacctg cgcggcaagg
      541 tgaaggtgca tggcgagacc ctcgaacccg ctcatcgcgg cggttgatct ctccgacctc
      601 gcggaggcgg agcgtctcgg cggcgccctc gcggggacgg tcgggcacct caaggtcggg
      661 ctcgagctgt tcacggccgc cggccccccc gccgtggagc gtctcgggcg ccacgcgccg
      721 ctgttcctcg acctcaagct gcacgacatc ccgacgacgg tcgagcgcgc cgcgcgcaac
      781 gtcgggcgcc tcggcaccgc gatgctcacc gtccacgcgc tcggcggccc cgcgatggtg
      841 gccgcggcgg tcgcgggcgc ggagcgaggg gcggccgacg ccggcgtcga accgccggcg
      901 atcgtcgccg tgaccgtcct gtcgagcctc gccggcgagg ggctagcgct cccgtcctcg
      961 ctcgcctacg aggccgtcga cgcgggggcg cgtggcgtcg tcgtctccgg tgacgacgtc
     1021 cggcaggtcc gcgaggtcct cggcagcgga ccgctcctga tcgtccccgg gatccggccc
     1081 tcggcacatc cgacgaacga ccatgcgcgg gtcctctcgc ccggcgaagc gctcgaggcg
     1141 ggcgccgacc acctcgtgat cggccgtccg atcacgggcg cccccgaccc ggccgaggcc
     1201 gcccgcgcga tcctccggga gatcacctga acgagcgcga gaccctcgac ctcctccgtg
     1261 agaagggggc cgtcctcgat ggtcacttcc tcctgacgtc ggggcggcac tcgccgacct
     1321 acgtcgagaa ggcgcggatc ttcgaacacc cggcggcggt cgagcggctc gccgccgagg
     1381 tggcggcctg gcacgggggg gtcgaggccg tggtctcgcc cgccgtcggt gcgatcccgt
     1441 tcgggttcgc ggtggcgctc gcggccggga gccggttcct ctacgcggag cggcaggacg
     1501 gggcgatgac gttgcggcgt gggttctccc tgcgggcggg ggagcgcgtc ctcgtcgtcg
     1561 aggatgtcgt gacgacgggg gggtcggccg cggaggtcgc cgccctcgtc cgtgacagcg
     1621 gcgcggagct cctgggcgtc gcggcgctcg tcgaccgatc cgacggcacg accggactgc
     1681 cgctgcgggc gctcgtccgg atcgaggcgc agacctacgc gcccgaggaa tgtccgctct
     1741 gccgcgacgg acgaccgctc gagtcgcacg gctcgcgcca tctcgcctga ttgcccgcgc
     1801 gaaaaagcgc cggatataat ccggttcccc cacgccagag gaggagacgc atgccgctgc
     1861 cgaccctgac ccccgagcaa cgacgcgagg ccctcgcgaa agccgccgag gcccggaaga
     1921 agcgtgccga ggtgaaggat cggctcaaga gcggcagcat gagcctcaag gacgtcctcg
     1981 ggaagcagtc cgacagcgtc gtcggcaaga tgaaggtctc caccgtcctc gagtccttgc
     2041 cgggcgtcgg gaaggtccgg gcgcggcgga tcatggagcg gctcgacatc tcctcgagcc
     2101 ggcgggtccg cggcctcggc acgaagcagc gcgaggcgct cgccgccgaa ttcgcgaaca
     2161 agtagcggcg acatcgcggc acgcgggcgg ctcttcgtca tctccggtcc ctccggcgtc
     2221 ggcaagggga ccgtcgttcg acgtctgatc gacctgcgcc ccgacctcgc gttctccgtc
     2281 tcctacacga cgaggcgccg ccggccgacc gagcgtgagg gccgtcatta ccgcttcgtc
     2341 gaccgggagg ccttcgaccg gctcatcgag gacgggcagt tcctggagtg ggcggaggtc
     2401 ttcggcgagc gctacggcac gccgtcggcg gaggtcgagg tcgcgcgccg tgccgggcgc
     2461 gacgtgctgc tcgaggtcaa tatggagggc gcccgctccg tccgggctcg cgtgccggac
     2521 gccgtgctcg tgttcctcgc gccgccctcc gaggaggagc tggcgcggcg actctccgcc
     2581 cgcggcaccg aatcgtcgca ggagctgcag cgccgtctcc gcgaggctcg ccgcgagatg
     2641 gccgaggcgc cggcgttcga ccacgtcgtg ctgaacgacg aggtggaacg ggccgcggcg
     2701 gcgatccttg ctatcatcga tgcgaccccc cccgcagccg agggagacgt gtgatgatcg
     2761 aaccgagcat cgaccaactg atggacaggg tcgactccaa gtacaccctg gtgatcctcg
     2821 ccgccaagcg cgcgcgggag atcaactcgt actacagcca gctcggggag ggccgcgggg
     2881 agttcgtgcc gccactcgtc gagaccggcg gccttcgcaa caagccgctc tcgatcgcga
     2941 tggaggagat cgccgacgac aaggtgacct tcgagcggtc ggagccggca cccgacgacg
     3001 aggagtaacc ggtggaaggc ccgctcgccg ggcgcaccgt cctcctcggg gtcaccggag
     3061 ggatcgccgc gtacaaggcc gccctgctcg cccggctcct gcgggaggac ggcgccgagg
     3121 tccaggtcgt gatgacgccg tccgcgacgc gcttcatcgg ccccgacacc ttcgcggccc
     3181 tcaccggccg gtccgtccac tccgacgtct tcgagcgccc cgaggcggtc ctgcacgtgc
     3241 ggctcgcgcg cgaggccgat ctggcggtgg tcgcccccgc gaccgcgaac ctgctggccc
     3301 ggctcgcgct cggcctggcc gacgacctcc tcagctccac cctcctcgag cttcgggcgc
     3361 cggtcgtcgt cgccccggcg atgcacaccg ggatgtggtc ctccgcggcg acccggacga
     3421 acctcgcgac gctgcgcgat cgcggcgtcc ggatcgtcgg cccggtcgag ggcgcgctcg
     3481 cagccggcga ccgcgggatg ggccgcatgg ccgaacccga cgagatcctc gcggaggtcc
     3541 gcgccgccct cgcgggcccg ggggacctgg ccggccgacg cctcctcgtc accgccgggc
     3601 cgacgcacga gccgatcgac cccgtccgct tcatcgggaa ccggtcgagc gggaagatgg
     3661 gctacgccgt ggcggcggcg gccgcccgcc ggggcgccgc cgtgacgctc gtcagcggcc
     3721 ccacgcggct cgacccgccg cccggcgtcg aggtcgtgcg cgtcgagacc gcggaggaga
     3781 tgcgctcagc ggtcctggct cgcctcgacg actcggacgc cgtcgtgaag gcggcggccg
     3841 tggcggactt ccggccggcg cggagcgcga cacgcaagct gaagaaggag gcgggcgtgc
     3901 ccgacctcgg cctcgagccg acgccggaca tcctgcgaga ggtcggggaa cgggcgggag
     3961 agcgcgtcct cgtcgggttc gccgcggaga ccgaggacct cgagaccgcg ggccgccgca
     4021 agctcgcgga gaaggggctg gacctcgtgg tcgtgaacga ggtcggctcc ccggggacgg
     4081 ggttcgacgc ggacacgaac cgggcgatga tcctctccgc cggcggcgac gacgagcccc
     4141 tccgcgactg gacgaaggtc gagctcgccg aggcgatctg cgaccgcatc gcgaagcgcc
     4201 tcgcctgaag ccttcgcgtt gtgtgcacct gcacaaaggg ttcccgccgc cgcgcgtcgt
     4261 agatagagcg cgtgaagctc gctttccgct ccctgcttcc cctcgccctg gtgttcggca
     4321 tcccgggggt gccggccacg gctggcggag gcctgctccc cgacggcccc gggctcgagt
     4381 cggaggggga catcgtccac gtcgccaacg tctcctacgg cggtggctcc gacctggagt
     4441 tcgccacgaa gacggtgcgg gtcctcgacg agaacgggga gcccgtctgc gcgcgcgacg
     4501 agcggggcga gtgcgtccgc gatgcgaacg gcgacgtggt ctacgagacc gaggagcgcg
     4561 actacgcctt cgccggaagg ctctcgcagc cggtccaggt gatcgacatc accgacccgg
     4621 ccaagccggt tcgcgtggcc gaggtcccgt gccgcttcaa ccagaacgac gtccaggtct
     4681 tcggggacac gctgctcatg gcggccgacg cgagcggtcc ctgcacgatg gcgaacgggg
     4741 cgacgacgaa cggcgccggc ttcgccaccg ccgacatctc ggatccgcgc ttccctcggg
     4801 tgatcgggcg cgcgaaccag cccggcggcg cgcacaccct caccgtccat ccgtcgcggc
     4861 cgctcgtcta cgtctcgaac ggcgacatct cggtgaacgg atcgatccac atctggagca
     4921 tcgcgaaccc cgccaacccc gtgaaggtga gggactgggc gccacagggg gcggtcgaac
     4981 cgccgcacga catcaccttc aacgcgacgg ggacgcgcgc ctactcggcc gcccaggacg
     5041 cccacacgtt catcctggac acgacggacc cgcagaaccc ctcgacgatc tcggtgatcg
     5101 tgaacgaggg gatctcctac tcccaccagg ccgaccccac ccccgacggg aaatacctgc
     5161 tgatcggcga cgagctcggc ggcggcgcgg cgacccttcc gtcgcccggg ggcccggtcc
     5221 acgtctacga catccgcaac gagcagcggc cggtgaaggt cggtgtgatc ggcaccgacg
     5281 ccaccggcgt gaacggcgtc tcgacggccc acgtcttccg gatcaacccc gacggctgga
     5341 cgatggcgat cggctggtac cgcgacggcg tccacgtctg ggac
//
LOCUS       DATLGG010000200         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_91558, whole genome shotgun sequence.
ACCESSION   DATLGG010000200 DATLGG010000000
VERSION     DATLGG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91558"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1545
                     /locus_tag="VM638_05700"
     CDS             148..1545
                     /locus_tag="VM638_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM11952.1"
                     /translation="MRATGTGSLPRKATAAVAATTQQQITRLVFLLTLGVVALALLLA
                     LLAARRITQPVVALTQAARRVQAGDLAAKADVKARDEVGDLAGAFNQMTESVTEATEK
                     LRAAAEEEARLRTRLETVLNSMGDGLLAVNEAGAVVTYNPAAAAMIGLPERRVIGREV
                     REVVSGRDVEGRDVDFGDEEVPGLAFIRRDDGREVPVAITSSPLRNSAGVLLGRVLVL
                     RDMSREHEVERMKTEFLANVSHELRTPLTPIIGYSEIMARRGVPPERSQEFAGSILDS
                     ARRLERIVAMLVDFSAMEGGRMSIDAQPTSVRKLVKTAVDEWRERSSSHRFVTKFEQG
                     LPEAEVDTSLMRRALDELLDNAVKYSPNGGNIRVSVSSPNSRSRRILRIDVEDEGIGI
                     ERDDLAGVFEDFRQVDASDTRTFGGLGLGLAFVRRIVEAHRGTIGVESQPGQGSSFSI
                     TIPAADTSKEKAEDA"
     gene            1542..>2460
                     /locus_tag="VM638_05705"
     CDS             1542..>2460
                     /locus_tag="VM638_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11953.1"
                     /translation="MRRTTAAAVILGMVAAACARSEASEQERPRLVPMSGGVQVLNGE
                     SWEPVSDEVELGPDMQVRVGRSSRARLELPAGQTLELARDARVAVDDVLAPKVLAGSV
                     LAYADQGLTLDVESFKVFGRQSVFRVDLSLPQRVAVYEGAATLPGSGWESPVGALQQV
                     GVIAGAVLRGPQPLQVRPDDPWDIRLLSEAIDVGAGLLRQETGLGRQLAGDRVRTAIL
                     RALGDEVGEDELADLLDEAGAAEVVVAAVVAMTAGRPDLGTAIAEILELRRLGASWFV
                     VAQRFGVSRTVLTSVTEVVGRILDTVERLE"
BASE COUNT          360 a          815 c          923 g          362 t
ORIGIN      
        1 ggcgtgtagg ccacgaagta gcgctggccg ccgatgcggg cgaccccttc ggccgtcgcg
       61 tggttggcga ggaagtcctc gagcagaccc gagagccgat cctcggggat catgggcgac
      121 ccgagctggt agcccgggaa cgtaccggtg agggccacgg ggacggggtc gcttccccgg
      181 aaggcaacgg cggcggtcgc ggccacgacc caacaacaga tcacgcggct cgtgttcctg
      241 ctgacgctcg gggtggtcgc tctcgcgctc ctcctcgcgc tcctcgccgc gcggcgcatc
      301 acgcagcccg tcgtcgccct cacgcaggcc gcccgccggg tgcaggcggg tgacctggcg
      361 gcgaaggcag acgtgaaggc gcgcgacgag gtcggtgatc tcgccggcgc cttcaaccag
      421 atgacggagt ccgtgacgga ggcgacggag aagctccgcg ccgcggccga agaggaagcg
      481 cgcctccgca cccgactcga gaccgtcctg aactcgatgg gcgacggcct cctcgcggtc
      541 aacgaggccg gcgcggtggt cacctacaac ccggcggccg cggcgatgat cggccttccc
      601 gagcgccgcg tgatcggtcg ggaggtccgc gaggtggtga gcgggcgcga cgtcgaaggg
      661 cgcgacgtcg acttcgggga cgaggaggtc cccggcctcg cgttcatccg ccgggacgac
      721 ggccgtgagg tgcccgtcgc gatcacctcg tcaccgctgc gcaacagcgc gggggtgctg
      781 ctcggtcgcg tgctcgtcct gcgtgacatg tcgagggagc acgaggtcga gcgcatgaag
      841 acggagttcc tcgcgaacgt gtcgcacgag ctgcgcacgc cactcacccc catcatcggc
      901 tactccgaga tcatggcgcg gcgcggggtc ccgccggagc gcagccagga gttcgccggc
      961 tcgatcctcg actcggcgag gcgcctcgag cggatcgtcg ccatgctcgt cgacttctcg
     1021 gcgatggagg gcgggcggat gtcgatcgac gcccagccga cctcggtccg gaagctcgtg
     1081 aagaccgccg tggacgaatg gcgggagcgt tcctcgagcc atcgcttcgt gacgaagttc
     1141 gagcagggtc tccccgaggc cgaggtcgat acctcgttga tgcggcgcgc cctcgacgag
     1201 ctcctcgaca acgcggtgaa gtattccccg aatgggggga atatccgggt ttctgtctct
     1261 tccccgaact cccgatccag gcgtatcctg aggatcgacg tcgaagacga aggtatcggg
     1321 atcgaacggg acgacctggc gggggtcttc gaggacttcc ggcaggtgga cgcctcggac
     1381 accaggacct tcggcggatt ggggctgggg ctggcgttcg tgcgacggat cgtcgaggcg
     1441 catcgcggga cgatcggcgt ggagagccaa ccgggtcagg gaagcagctt cagcatcacc
     1501 atccctgcag ccgatacatc caaggagaag gcggaggacg cgtgaggcgg acgacagcgg
     1561 cggcggtgat cctggggatg gtggccgccg cctgcgcgcg ctcggaggca tccgagcagg
     1621 agcgcccccg cctcgtcccg atgagcggcg gcgtccaggt tctgaacggc gagtcgtggg
     1681 agccggtctc cgacgaggtc gagctgggtc ccgacatgca ggtgcgcgtc ggccgctcga
     1741 gcagggcgcg cctcgagctc ccagcgggac agaccctcga gctcgcgcgc gacgcgcggg
     1801 tcgccgtcga cgacgtcctc gcgccgaagg tgctggccgg gagcgtcctg gcctacgccg
     1861 accagggtct gacgctcgac gtcgagtcgt tcaaggtctt cggccggcag tccgtgttcc
     1921 gcgtcgacct cagccttccg cagcgcgtcg ccgtctacga aggggccgcc acccttccag
     1981 ggtcgggctg ggagtcgccg gtcggcgcgc tccagcaggt cggggtcatc gccggggcgg
     2041 tcctgcgcgg accgcaaccg ctccaggtcc ggcccgacga cccttgggac atccggttgc
     2101 tcagcgaggc gatcgacgtc ggcgcggggc tgctgcgcca ggagacgggt ctgggccggc
     2161 agctcgcggg tgatcgggtc cgcacggcga tcctgcgggc cctcggcgac gaggtgggtg
     2221 aggacgagct cgcggatctc ctcgacgagg cgggagcggc cgaagtcgtc gttgctgccg
     2281 tggtcgcgat gaccgcgggg cgtccggacc tcggaaccgc catcgccgag atcctcgagc
     2341 tgcggcgact cggggcatcg tggttcgtcg tcgcgcagcg gttcggcgtc tcccggacgg
     2401 tcctcacatc cgtgactgag gtcgtcggca ggatcctcga cacggtcgag cggttggagg
//
LOCUS       DATLGG010000201         3319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_212489, whole genome shotgun sequence.
ACCESSION   DATLGG010000201 DATLGG010000000
VERSION     DATLGG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212489"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VM638_05710"
     CDS             complement(<1..200)
                     /locus_tag="VM638_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11954.1"
                     /translation="MRRWLPIVAILLATLPAPSLGGPPPEEELAELLRERAEAMRRGD
                     LETYLASVAPDVPDFRRRQRRL"
     gene            324..2162
                     /locus_tag="VM638_05715"
     CDS             324..2162
                     /locus_tag="VM638_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11955.1"
                     /translation="MQLALRTIIPVILAVALLPSPLGATATTTDAETGAERSTRAPRA
                     ALFHAFQRSDDPAGDVRDAVRSRMGDLVGIPTSSSSGPIYDGEAYLLNGGDFDGDGRD
                     DGLEISFLLGNRFMTEIAAVRATDGAVLWQQAVEGFAWARAAGDLDGDDLADIFVFDD
                     TYEPAYAGASDVDAYSEVYQITTRAVSVDRGADIWAAPRSGTGAFAYIDHDWERGVAA
                     IAGASFPVSDGHDLDGDGATDLLVGSFAGAYAWDDEAETFNGVDAVSSFELVSGRTGA
                     SRTVFPASAGYAEIAAVPDLTGDDLPDVAILTLGGLAPSVAAYPGTGGPRKWSAGVPE
                     DLSWAWPSGADLDGDGTGDVLVHGRSLAWDDEEEDYLVSSQRLSALSGIDGTTAWTAE
                     TPPWSDGVVAGDATGDGGDDVLVVTSEDDDRSLFGHHVGGGDEMWLLHGDDGSTVWKR
                     RIARLRWMSVAGDLTGDGVIDALVHRFARLTVNGRRRTVRAVSVYSGVTGSARWTVPA
                     TRGWMLPLGGDLNDDGAGDLAWFKGGGAYRAMSGRTGRSLWRRDVGTGGYLLLLGVAR
                     TNEGGDILESAIGSGWSSMITSMRAGDTGSLWWRRTRPFDGWGGEG"
     gene            complement(2170..2625)
                     /locus_tag="VM638_05720"
     CDS             complement(2170..2625)
                     /locus_tag="VM638_05720"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HVM11956.1"
                     /translation="MAENRDERLDRELIELLQELRVAIPGVQVMFAFLLMVPFTERFG
                     QVDALERAVYLGAFLATAVASVLFIAPTAYHRLRWREYDKERMLVTSNRLTIAGTIFL
                     ALAVAGVVFVVTDQVLGARAAAATVGVAVALLVWFWFGLPLSRKRSDRA"
     gene            complement(2632..2805)
                     /locus_tag="VM638_05725"
     CDS             complement(2632..2805)
                     /locus_tag="VM638_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11957.1"
                     /translation="MTYDPHHVPPAHAHHEEPVRESTGLTGYAIVKYGFILLITIVVL
                     YFIARYIIPLLTR"
     gene            complement(2865..>3319)
                     /locus_tag="VM638_05730"
     CDS             complement(2865..>3319)
                     /locus_tag="VM638_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM11958.1"
                     /translation="WALRVPRRLQETGLKVSKAVSQLSQELGRSPRVSEIAKATALTE
                     EEVLEGMDTIHAYTTSSLDAPTDEDGTTTAQKLGSEDETLELLEGWTSVAPALKALPE
                     RERRIVYLRFFRGMTQTQIADELGISQMHVSRLLARTLRTLRDSVTGD"
BASE COUNT          498 a         1141 c         1195 g          485 t
ORIGIN      
        1 aagagccgcc gctggcggcg ccggaagtcg gggacgtcgg gagcaacgct ggcgaggtag
       61 gtctccagat cgcctcgccg catggcctcc gcacgctcac ggaggagctc ggcgagttcc
      121 tcttccggcg ggggacctcc gaggctcggc gccgggagcg tggcgagaag gatcgccacg
      181 atgggaagcc atctgcgcac gtggtcaccc tatcgcgggg cggaggccga tctctgtgca
      241 ggtgcacaaa gggatgggtg gagcggtcag cgaactagat acatcaggag ggcgactcca
      301 tcgcccgacc caggaggtcc ctcatgcagc tcgcgctccg caccatcatc cccgtgatcc
      361 tggccgtcgc gctgcttccg agcccattgg gcgccacagc gacaacgacc gatgctgaga
      421 ccggcgcgga gcgatccacc cgcgctcctc gcgccgccct cttccacgcc ttccagcggt
      481 cggacgatcc tgccggcgat gtccgggatg ccgtgcggtc gcggatgggc gacctcgtcg
      541 ggatcccgac ctcctcgtct tccggcccca tctacgacgg cgaggcgtac ctgctgaacg
      601 gaggcgactt cgacggggac ggccgcgacg acggcctcga gatcagcttc ctgctcggca
      661 accggttcat gaccgagatc gccgcggtcc gcgccaccga cggcgcggtg ctgtggcagc
      721 aggcggtgga gggattcgcc tgggcgaggg ctgccgggga cctcgacggc gacgacctgg
      781 ccgacatctt cgtcttcgac gacacgtacg agccggcata cgccggcgcc tccgacgtcg
      841 acgcctacag cgaggtctac cagatcacga cgcgcgcggt gagcgtcgac cggggagcgg
      901 acatctgggc ggccccgcgc agcgggactg gcgccttcgc ctacatcgac catgactggg
      961 agcgtggcgt cgccgcgatc gcaggcgctt ccttccctgt atccgacggc cacgacctcg
     1021 acggagacgg ggcgacggac ctcctggtgg gcagcttcgc cggcgcctac gcgtgggacg
     1081 acgaggccga gacgttcaac ggcgtggacg ccgtgagcag cttcgagctc gtctccggca
     1141 ggaccggagc ctcccgtacc gtcttcccgg ccagcgccgg gtacgcggag atcgccgccg
     1201 ttcccgacct gaccggcgac gacctccccg acgtcgccat cctcacgctc gggggcctgg
     1261 cgccctcggt ggcggcctac ccggggaccg gcggtccccg caagtggtcc gcgggggtcc
     1321 ccgaagacct gagttgggca tggccctccg gagccgacct cgacggcgac gggaccggcg
     1381 acgtcctcgt gcacggccgt tcgctggcct gggacgacga ggaggaggac tacctcgtca
     1441 gctcgcagcg gctcagcgcg ctgtccggca tcgacggcac gacggcctgg acggctgaga
     1501 ccccgccgtg gagcgacggc gtcgtcgccg gagacgcgac cggagacggg ggcgatgacg
     1561 tcctcgtcgt cacctcggag gacgatgacc gctccctctt cgggcatcac gtcggcggcg
     1621 gcgacgagat gtggctcctg cacggcgacg acggctcgac ggtctggaag cgccggatcg
     1681 cacggctccg gtggatgagc gtcgccgggg atctcaccgg tgacggcgtg atcgacgccc
     1741 tcgtccatcg gttcgcccgc ctcacggtga acgggcggcg ccggaccgtc cgcgcggtga
     1801 gcgtctacag cggggtgacc gggtccgctc ggtggaccgt cccggcgacg cgcggctgga
     1861 tgcttccgct cggtggcgac ctgaacgacg acggggccgg agacctcgcc tggttcaagg
     1921 gcgggggcgc ctaccgcgcc atgagcggcc ggaccggacg gtcgctgtgg aggcgagacg
     1981 tcggaacggg gggctacctt ctgttgctgg gtgtcgcccg cacgaacgag ggcggtgaca
     2041 tcctcgagtc cgcgatcggg tccggatggt cgagcatgat cacgtcgatg cgcgccgggg
     2101 acacgggctc gctctggtgg cgcaggacgc gaccgttcga cggctggggc ggagaaggct
     2161 agcgccgcct caggcccggt ccgagcgttt ccgcgagagc ggcagcccga accagaacca
     2221 gacgagcagc gccaccgcga cgcccacggt cgccgcagcg gctcgggcgc cgagcacctg
     2281 atccgtcacg acgaacacga cgccggccac cgccagcgcg aggaagatcg ttccggcgat
     2341 cgtcagccgg ttcgacgtca cgagcatccg ctccttgtcg tactcccgcc accgcagccg
     2401 gtggtaggcg gtcggagcga tgaacaggac cgacgcgacc gccgtcgcga ggaacgctcc
     2461 caggtagacg gcgcgctcca gcgcatcgac ctgaccgaac cgctccgtga acggaaccat
     2521 caggaggaac gcgaacatca cctggacgcc cgggatcgcg acgcggagct cctgcaggag
     2581 ctcgatcagc tcgcggtcga gccgttcatc gcggttctcg gccacgcgcg gctagcgggt
     2641 gagcagcggg atgatgtagc gcgcgatgaa gtacaggacg acgatcgtga tgagcaggat
     2701 gaacccgtac ttcacgatcg cgtaccccgt gagccccgtc gactcgcgca ccggctcttc
     2761 gtggtgggcg tgggcgggtg ggacgtggtg cggatcgtag gtcatggtcg tctcccttgt
     2821 ctcgcttcgc tcggcaggat tccctcgctt cgctcgggcg aaactcagtc gccggtcacg
     2881 ctgtcgcgca gggtccggag ggtccgcgcg agcaggcgcg aaacgtgcat ctgcgagatc
     2941 ccgagctcgt cggcgatctg ggtctgggtc atgccccgga agaaccggag gtagacgatc
     3001 ctgcgctcgc gctcggggag cgccttgagc gcgggggcga ccgaggtcca cccctcaagg
     3061 agctcgagcg tctcgtcctc gctgccgagc ttctgcgcgg tggtcgttcc atcctcgtcg
     3121 gtgggagcgt cgagcgagga tgtcgtgtag gcgtggatcg tgtccatccc ctcgaggacc
     3181 tcttcctcgg tcaacgcggt cgccttcgcg atctcgctga cgcggggcga ccgaccgagc
     3241 tcctgcgaga gctgggagac ggccttgctc accttcaggc cggtctcctg gagacggcgc
     3301 ggcacgcgca gcgcccacc
//
LOCUS       DATLGG010000202         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_6538, whole genome shotgun sequence.
ACCESSION   DATLGG010000202 DATLGG010000000
VERSION     DATLGG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6538"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VM638_05735"
     CDS             complement(<1..1779)
                     /locus_tag="VM638_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11959.1"
                     /translation="MRGSFLRRVFTVTVAGAIWMVGLGAVPAGAHPEAHCEKEASTTS
                     SLGAQLLEELVAGSHNDCTAEGIDVSESTGGALGEPVEEAVEGDSFGGAYEWSANMEP
                     IGFSKRHVPTSGTGANLYNSDLAFWGDLAFQGTYAGFRIIDISDPTDPVQLVNYTDCA
                     SSAGQGDLVVWEHLLIRSWDAPASTSATCGGQPVGSGFEGIHIFDISNPSNPVFIRGL
                     RMASNTTPAGCGSHTATAVPDPARGNLYLYVGGSSSSCTGMDIVRISMSDPQFAVYLR
                     RASASRQCHDNNVIMGDVNLAVCAGGNGFSVFSFDPSIDPEAVGGLANPRLLYSMPVS
                     GVSIGHSASFSYDGSILIFGHEPGGGSQAQCQSTSSVTNRTIYFFEPRTGAALGSYVH
                     PRPQSAQENCTWHNFNVVPSDHGHVFVSGNYQSGILVVDFTNPATPHLVAHADPARLG
                     TGSGITLGGDWSTYWYDGLIYESDIRRGLIVWKLEDDEGRPLVGRARTFGRSNPQTQD
                     VTFSWDRTAPRIDIASPVDGASYAAGSEVVADYDCTDEQGGSGVVSCEGTAADGEALD
                     TSSPGEYTFTVEASDLAGNTTTRSVTY"
     gene            1982..>3248
                     /locus_tag="VM638_05740"
     CDS             1982..>3248
                     /locus_tag="VM638_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11960.1"
                     /translation="MTAPSDAGAHRGDAYHALRQRHLQEMRQLLPEHLDRVAWSREEL
                     AAERLRRLRHLLRVAKARSSWHRERLSGVDPERFREEDLASVPAMTKEELMESFDDVL
                     TEPSLTLERVEDHLDDLEEGPAYLPGGYQAITSSGSSGRRGVFVYDFRGWTEKFLGWI
                     RYLVREIGTGKRFAIVAGGSPSHGSRLLLQTFSDPEVFDVRPLPTVLPQERIVQGLNA
                     LQPEVLAGYATAIGALSPAAAEGRLRIQPRAVVTGGEPLLPELRALIEQAWRVPVHDW
                     WLSSEAGAMGIGCGRGDALHLSDDLLIVEPVDRHGNPVPPGEAAAKIYVTNLFNTALP
                     LIRYEMSDEVTLLPETGTACGCGSQHRRVAAVQGRVDDAFVYAGGPIVHPHVFRAPLG
                     RQRDVIEYQVRQTSDGAAVSIVTGGRTDLD"
BASE COUNT          490 a         1143 c         1108 g          507 t
ORIGIN      
        1 gtacgtcacg gaccgggtcg tcgtgttgcc cgccaggtca ctcgcctcga cggtgaacgt
       61 gtactcgccc ggcgagctgg tgtcgagcgc ctcgccgtcg gcagccgtac cctcgcagct
      121 gacgacgccg gatccgccct gctcgtcggt gcagtcgtag tcggccacga cctccgagcc
      181 ggcggcgtag gacgcgccgt cgacggggga ggcgatgtcg atccgcggcg cggttcggtc
      241 ccaggagaag gtcacgtcct gcgtctgcgg gttcgaacgg ccgaacgtcc tcgcgcggcc
      301 cacgagcggc cgaccctcgt catcctcgag cttccacacg atgaggccgc gccggatgtc
      361 cgactcgtag atgagcccgt cgtaccagta cgtcgaccag tcgccgccga gggtgatccc
      421 actgccggtc ccgagtcggg cagggtcagc gtgcgccacc agatgcggcg tcgccgggtt
      481 cgtgaagtcg acgacgagga tgcccgactg gtagttgccg gacacgaaga cgtggccgtg
      541 atccgacggc accacgttga agttgtgcca cgtgcagttc tcctgcgcgc tctgcgggcg
      601 cgggtggacg taggacccca gcgcggcgcc ggtgcgcggt tcgaagaagt agatcgtgcg
      661 gttcgtcacg ctgctcgtgc tctggcattg cgcctggctc ccgccgccgg gctcgtggcc
      721 gaagatcagg atgctgccgt cgtagctgaa cgaggccgag tggccgatcg agacgccgct
      781 caccggcatc gagtagagga gcctcgggtt cgcgaggcct ccgacggctt cagggtcgat
      841 gctcgggtcg aagctgaaga cggagaaccc gttgccaccc gcgcagaccg cgaggttgac
      901 gtcgcccatg atcacgttgt tgtcgtggca ctgccggctc gccgacgctc gacgcaggta
      961 gaccgcgaac tgcgggtccg acatcgagat ccgcacgatg tccatcccgg tgcatgagct
     1021 gctcgatcca ccgacgtaga ggtaaaggtt gccgcgcgcc gggtccggaa cggccgtcgc
     1081 cgtgtgggat ccgcacccag ccggcgtggt gttcgaggcc atccgaagcc cgcggatgaa
     1141 gaccgggttg gaggggttcg agatgtcgaa gatgtggatc ccctcgaaac cgctgccgac
     1201 gggctgtccg ccgcaggtcg cggatgtcga cgcgggcgcg tcccaggagc ggatcaggag
     1261 atgctcccag acgacgaggt caccctgacc cgcgctgctc gcgcagtcgg tgtagttgac
     1321 cagctgcacc gggtcggtcg ggtcggagat gtcgatgatg cggaaccccg cgtacgtccc
     1381 ctggaaggcg agatcccccc agaacgcgag gtccgagttg tagaggttcg cacccgtccc
     1441 gctggtcggc acgtgacgct tcgagaagcc gatcggctcc atgttcgccg accactcgta
     1501 ggcgccgccg aagctgtcgc cctcgacggc ctcttcgacc ggctcgccca gggcgccgcc
     1561 ggtgctctcg gacacgtcga tcccctcggc ggtgcagtcg ttgtgcgatc ccgccaccag
     1621 ctcctcgagg agctgcgccc cgaggctgga cgtcgtggac gcctccttct cgcagtgcgc
     1681 ctccggatgc gcgccggccg gaaccgcgcc gaggcccacc atccagatgg caccggccac
     1741 cgtcaccgtg aagacgcgcc gtaggaacga accccgcatc gataccccct cccatcgccg
     1801 ccggcgacgc gcatgcgcac ccctgccgga cctgtggtct ctggcgtctg tattcgacgt
     1861 accgccccac cctcctgcct ggcggagggc gttcccgacg cgcggacgca ggccagaggg
     1921 gcattgtcgc ggcatcggcc cagacgcata atccagccat gtccgtcact gatgccgggc
     1981 cgtgactgct ccctcagacg ctggggccca tcgtggcgat gcctaccacg ccctgcggca
     2041 gcggcacctc caagagatgc gccagctctt gccggagcat ctggaccgtg tggcgtggtc
     2101 gcgtgaggag ctggccgcgg agcgcctccg ccgcctccga catctcctcc gggtcgcgaa
     2161 agcgcgatcc tcctggcacc gggagcgcct gagtggggtc gatcccgagc ggttccggga
     2221 ggaggacctc gcctcggtcc ccgcgatgac gaaggaggag ctcatggaga gcttcgacga
     2281 tgtgctcacc gagccgagcc tgacgttgga gcgggtcgag gatcatctcg atgatctcga
     2341 agagggtccg gcctacctgc ccggcggcta tcaggccatc acctcatcgg gatccagcgg
     2401 gcgccgcggg gtcttcgtct acgacttccg cggctggacc gagaagttcc tcggctggat
     2461 ccgctacctc gtccgcgaga tcgggacagg gaagcgcttc gcgatcgtgg ccggaggctc
     2521 gccctcccac ggctcccgcc tgctcctcca aacgttctcc gaccccgagg tattcgacgt
     2581 tcgaccgctc ccgaccgtgc tgccgcagga gcgcatcgtc caggggctca acgcgcttca
     2641 gcccgaggtc ctcgccggct atgcgacggc gatcggggcg ctttcgccgg cagccgcgga
     2701 agggcgcctg cggatccagc cgcgggcggt cgtcaccggc ggcgagccgc tcctgcccga
     2761 gctccgtgcc ctcatcgagc aggcctggcg cgtgcccgtc cacgactggt ggctgagctc
     2821 ggaggccggc gcgatgggga tcgggtgcgg acgcggggac gccctccacc tgagcgacga
     2881 cctgctgatc gtcgagccgg tcgatcgcca cgggaacccg gtgcccccag gcgaagcagc
     2941 agccaagatc tacgtcacga acctcttcaa cacggccctc cccctgatcc gctacgagat
     3001 gagcgacgag gtgacgctgc tccccgagac gggaaccgcc tgcggctgtg gatcgcagca
     3061 tcggcgggtg gcggcggtcc agggacgcgt ggacgacgcc ttcgtctacg ccggtggacc
     3121 catcgtccac ccgcacgtct tccgcgcccc cctcggccgc caacgggacg tcatcgaata
     3181 ccaagtccgg cagacctcgg acggcgccgc ggtcagcatc gtcaccggcg ggcggaccga
     3241 cctcgatg
//
LOCUS       DATLGG010000203         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_181501, whole genome shotgun sequence.
ACCESSION   DATLGG010000203 DATLGG010000000
VERSION     DATLGG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181501"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VM638_05745"
     CDS             <1..560
                     /locus_tag="VM638_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HVM11961.1"
                     /translation="LPVRRPPGLAHPAWQIFQELSEAMGRDMGLTSLDAIHEEMGEVL
                     APREAGAASATVPATTAPPGDRSEGELTLFSYPLLVDEGRLSVGAEELKEALEQEAFA
                     EVNPADAARLELVDGGRARVETERGAAELPVRVTDGVAAGSVFVPWNQPGLQANTLLA
                     GRLTAPVTLSPVGEPAMAAAGEEES"
     gene            560..1714
                     /gene="nuoH"
                     /locus_tag="VM638_05750"
     CDS             560..1714
                     /gene="nuoH"
                     /locus_tag="VM638_05750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVM11962.1"
                     /translation="MDLLDWLLLLGRVVVVFAALLVSVMLVIWIERKVVADMQTRLGP
                     KRAGPFGILITLADGIKLFFKEGIRPSRADRWVYPMAPVASLVPAFLAFSVIPFGRPV
                     ELFGREVPLQLADLNVGVLFFLAMGSLAVYGVVLAGWASGSSYPLLGGVRSTAQMISY
                     EVGMGLGLVAVLMYTGTMQTSEIVARQDVIWNIIPQAPAFLLFMAAAMAETNRAPFDL
                     PEAESELVAGYHTEYSGIKFAMFFLAEYLHMITIAGITVTLFLGGWRGPIFSFLPGLW
                     PVVWFVLKVSVVLFIFIWLRATLPRFRYDRLMNFGWKVLIPWGLAWIVATGAIVVLPD
                     VYGRDRFFRWALYFVGAFLVIAVIGPMLFPKNRRTREPFTPIARQGGGAP"
     gene            1711..2169
                     /gene="nuoI"
                     /locus_tag="VM638_05755"
     CDS             1711..2169
                     /gene="nuoI"
                     /locus_tag="VM638_05755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HVM11963.1"
                     /translation="MILDLLKGFGVTFRNMFRKPVTVAYPHEIKDLPPRGHIGRHLLN
                     RHENGLEKCIGCELCAWACPADAIYVQGEDNDPDEPVSAGERFASDYQINYLRCIMCG
                     LCVEACPTRALTLTSNFEMSWETREEAIFTKEQLLRPPPPLGTAPGTEQG"
     gene            2169..2762
                     /locus_tag="VM638_05760"
     CDS             2169..2762
                     /locus_tag="VM638_05760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVM11964.1"
                     /translation="MDLVAFWMIAPVSVGAAVLMLLQRNAVHAALLLVLNQFTLAVFF
                     VLLDAHFLAAVQVIVYAGAIMVLFLFVIMLLGVDRNEVLRETIRGQRPLAVFLGVLLV
                     AELVLAVRLGVGLLSGPIPEMVQEGGNVEAVAQVLFTRYVFPFEVTSILLIVAAIGAM
                     VLARQRKVPPGTRIPVNEAGPGEPGAPSPRDHLEAAR"
     gene            2759..3064
                     /gene="nuoK"
                     /locus_tag="VM638_05765"
     CDS             2759..3064
                     /gene="nuoK"
                     /locus_tag="VM638_05765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVM11965.1"
                     /translation="MRTPIGHYIILSGLLFSIGTVGVLVRRNALVIFMAIELQLNAVN
                     LSLIAFSRRLGTVDGQVLAFFSMVVAAAEVVVGLAIIVAIFRRRRTANVDDASLLRW"
     gene            3069..>3915
                     /locus_tag="VM638_05770"
     CDS             3069..>3915
                     /locus_tag="VM638_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVM11966.1"
                     /translation="MIENLWLAAAFPLAGAVLLLFFGNALRRVAGGLAAALVGGSFVV
                     ALLALRDFLAAAPQARQVSVKLFDWISVGSFQAPVEFRVDPLSMVMVLVVTGVAFLIH
                     VYSIEYMHGDPRYPRFFAYMNLFVFAMLTLVLADNFVLMFVGWEGVGLCSYLLISFWH
                     ERPSAAAAGKKAFIVTRIGDTAMLIGVFLIFAVMGSVRFEEVLPAAAGLAEGTAFVIS
                     LLLFAGAVGKSAQIPLHTWLPDAMEGPTPVSALIHAATMVTAGVYMVGRNAVLFSHAP
                     MVLEII"
BASE COUNT          479 a         1355 c         1369 g          712 t
ORIGIN      
        1 tgctcccggt ccgccgccca cccgggctcg cgcaccccgc ctggcagatc ttccaggagc
       61 tgtccgaggc gatgggtcgc gacatgggcc tgacgtcgct cgacgcgatc cacgaggaga
      121 tgggggaggt gctggcgccg cgtgaggccg gcgcggcgtc cgccacggta ccggcgacca
      181 cggcgcctcc cggggaccgg tccgagggag agctgacgct cttcagctac ccgctcctcg
      241 tggacgaggg ccgtctctcg gtgggagccg aggagctgaa ggaggcgctg gagcaggagg
      301 ccttcgccga ggtgaacccc gccgacgcgg cgaggctgga gctcgtggac ggtggacggg
      361 cacgggtcga gaccgagcgc ggcgcggccg agctgccggt ccgggtgacg gacggggtcg
      421 cggccggctc cgtcttcgtc ccgtggaacc agcccgggct ccaggcgaac acgctactcg
      481 ccgggcggct gaccgcgccc gtgaccctca gcccggtggg agagcccgcg atggccgccg
      541 ccggcgagga ggagagctga tggatctcct cgactggctc ctgctcctcg ggcgcgtcgt
      601 cgtcgtcttc gcggcgctcc tcgtctcggt gatgctcgtc atctggatcg agcgcaaggt
      661 cgtcgccgac atgcagacgc gcctcgggcc gaagcgcgcg gggccgttcg gcatcctcat
      721 cacgctcgcg gacgggatca agctcttctt caaggagggc atccggccgt cgcgcgcgga
      781 ccgctgggtc tacccgatgg cgccggtggc gtcgctggtc ccggccttcc tcgcgttctc
      841 ggtcatcccg ttcggccgcc cggtcgagct gttcggccgc gaggtccccc tccagctcgc
      901 ggacctcaac gtcggcgtcc tcttcttcct cgcgatgggc tcgctcgccg tgtacggcgt
      961 cgtcctcgcc gggtgggcct cgggctcgtc gtacccgctg ctcgggggcg tccgctcgac
     1021 cgcgcagatg atctcctacg aggtggggat gggcctcggg ctcgtcgcgg tgctgatgta
     1081 caccggcacg atgcagacct cggagatcgt cgcgaggcag gacgtgatct ggaacatcat
     1141 cccgcaggca ccggcgttcc tgctgttcat ggcggcggcg atggccgaga cgaaccgcgc
     1201 gccgttcgac ctgcccgagg cggagagcga gctcgtcgcc ggctaccaca cggaatattc
     1261 cggcatcaag ttcgcgatgt tcttcctcgc cgagtacctg cacatgatca cgatcgcggg
     1321 gatcaccgtg acgctcttcc tcggcggctg gcgcggcccg atcttctcgt tcctgccggg
     1381 gctctggccg gtcgtgtggt tcgtgctcaa ggtttcggtc gtgctcttca tcttcatctg
     1441 gttgcgcgcg acgctcccgc ggttccgcta cgaccgcctg atgaacttcg gatggaaggt
     1501 gctgatcccg tgggggctcg cgtggatcgt ggcgacgggt gcgatcgtcg tcctgcccga
     1561 cgtctacggg cgggaccggt tcttccgatg ggcgctctac ttcgtggggg cgttcctcgt
     1621 gatcgcggtc atcggcccga tgctgttccc gaagaaccgg cggacccgcg agccgttcac
     1681 cccgatcgcg cggcagggag gaggggcccc gtgatcctcg acctgctgaa gggcttcggc
     1741 gtgacgttcc ggaacatgtt ccgcaagccg gtgacggtcg cctacccgca cgagatcaag
     1801 gacctcccgc cccgcggcca catcggccgg cacctgctca accggcacga gaacggcctc
     1861 gagaagtgca tcgggtgcga gctctgcgcg tgggcgtgcc cggccgacgc gatctacgtc
     1921 cagggcgagg acaacgaccc ggacgagccg gtctcggccg gcgagcggtt cgcgagcgac
     1981 taccagatca actacctccg ctgcatcatg tgcgggctct gcgtcgaggc gtgcccgacg
     2041 cgggcgctca cgctcacgag caacttcgag atgtcgtggg agacgcggga ggaggcgatc
     2101 ttcacgaagg agcagctcct gcggccgcca ccgccgctgg gcacggcacc ggggacggag
     2161 caggggtagt ggacctcgtc gcgttctgga tgatcgcgcc cgtgagcgtc ggcgccgcgg
     2221 tgctcatgct gctccagcgc aacgccgtgc acgcggccct cctgctcgtg ctcaaccagt
     2281 tcacgctcgc ggtcttcttc gttctcctcg acgcgcactt cctcgcggcg gtccaggtga
     2341 tcgtctacgc cggcgcgatc atggtgctgt tcctgttcgt gatcatgctg ctcggcgtcg
     2401 accgcaacga ggttctgcgc gagacgatcc gcggccagcg cccgctggcg gtcttcctcg
     2461 gcgtcctgct cgtcgccgag ctggtgctcg cggtccggct cggggtcggt ctcctcagcg
     2521 gcccgatccc cgagatggtg caggaaggcg gcaacgtcga ggcggtggcg caggtcctgt
     2581 tcacccgcta cgtgttcccc ttcgaggtca cctcgatcct gctgatcgtc gccgcgatcg
     2641 gggcgatggt cctcgcgcgt cagcgcaagg tgcccccggg gaccaggatc cccgtgaacg
     2701 aggcggggcc gggcgagccg ggggcgccgt caccgcgcga ccacctcgag gcggcgcgat
     2761 gaggaccccg atcggccact acatcatcct ctcggggctg ctcttctcga tcggcaccgt
     2821 cggcgtgctc gtccgccgga acgcgctcgt gatcttcatg gcgatcgagc tccagctgaa
     2881 cgccgtgaac ctctcgctga tcgccttctc gcgccggctg gggacggtcg acggtcaggt
     2941 gctggccttc ttctccatgg tggtcgccgc tgcggaggtc gtcgtggggc tcgcgatcat
     3001 cgtcgcgatc ttccggcgcc ggcggacggc gaacgtcgac gacgcgagcc tcctgcgatg
     3061 gtgacccgat gatcgagaac ctctggctcg cggccgcgtt ccccctggcg ggagccgtcc
     3121 tgctcctgtt cttcggcaac gccctgcgcc gcgtggccgg tgggctcgcg gcggcgctcg
     3181 tcggcgggtc gttcgtggtg gcgctcctcg cgctccgcga cttcctcgcg gccgccccgc
     3241 aggcgcgtca ggtcagcgtg aagctcttcg actggatctc cgtcggctcg ttccaggcgc
     3301 cggtcgagtt ccgcgtcgac ccgctctcga tggtcatggt gctcgtcgtg accggggtcg
     3361 ccttcctgat ccacgtgtac tcgatcgagt acatgcacgg cgatccgcgg tacccgcggt
     3421 tcttcgccta catgaacctg ttcgtgttcg cgatgctcac gctcgtgctc gcggacaact
     3481 tcgtcctgat gttcgtcgga tgggagggcg tcgggctctg ctcgtacctc ctgatctcct
     3541 tctggcacga gcgcccgtcc gccgccgccg ctgggaagaa ggccttcatc gtcacgcgga
     3601 tcggcgacac cgcgatgctg atcggcgtct tcctgatctt cgcggtcatg ggctcggtgc
     3661 ggttcgagga ggtcctgccc gccgccgccg ggctggcgga ggggacggcg ttcgtcatct
     3721 ccctcctcct gttcgccggg gcggtgggga agtcggcgca gatcccgctg cacacctggc
     3781 ttccggacgc gatggagggc ccgacgccgg tctccgcgct gattcacgcc gcgacgatgg
     3841 tgaccgcggg tgtgtacatg gtcggccgga acgcggtgct cttctctcac gcgccgatgg
     3901 tgctcgagat catcg
//
LOCUS       DATLGG010000204         3693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_228657, whole genome shotgun sequence.
ACCESSION   DATLGG010000204 DATLGG010000000
VERSION     DATLGG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228657"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VM638_05775"
     CDS             <1..594
                     /locus_tag="VM638_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HVM11967.1"
                     /translation="ETAEHPGVMDAEQLDLAGFCVGTVARDATLGPHRVGEGDLLVGL
                     PSTGPHANGYSLIRRLVEGLDLEAPAPWSGRALAEELLEPTAIYVRPVLEAVRAGLVS
                     AAAHITGGGLVENLPRALPRGLGADLDRASWDVPEVFKFLQERGDIPEEDMARTFNLG
                     LGMILVVPDERVLDPLSSAGLEASVVGRVAAGEGVRW"
     gene            591..2132
                     /locus_tag="VM638_05780"
     CDS             591..2132
                     /locus_tag="VM638_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM11968.1"
                     /translation="MKRGVLTLRATRGPDLQEVRSRRSQLHRLLLVIVVLLVAAVVLF
                     SIAAEQLLHRALPFVGIVATRAGFAAVMLLFAFYVWRRERALSRAERALVEGRVLAAA
                     LSNRMHELSAVSEAGRAVSAVLSEKQVMQVILDSVRDLLDATEASVMLLDPETNVLRL
                     AAGWGIDEEVTRGTAVMVGEGIAGWVAESLEPVILNGDVRDPRFRGFVPKDRPVRSAM
                     SAPLRAGSTTVGVINVSVSGGGREYTEHDLRALTDFAEHAGAAIANARLFERERQASS
                     ELAALEQQRRDFLATVTHDLKTPLTSILGYVKLLRRMDDSLDSEQAQAFTSVIERQGQ
                     RILEMVEQLVEASKLEAGAPALARERLDLGRIIDEQVLAVGGLLGGRSVDVGLPDALP
                     PMYGDPQAVEHILMNLLENAVKYTPDGSSITVEVTPADGEVQVSVTDDGPGIPEDELP
                     RVFDRYRQATERTERGSVGLGLYIVRNLAEAHGGRAWAENAPGKGARITFTLPVRAER
                     KPAEV"
     gene            complement(2136..2339)
                     /locus_tag="VM638_05785"
     CDS             complement(2136..2339)
                     /locus_tag="VM638_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11969.1"
                     /translation="MRGKNLVTPDHVGRRVTFQYELPNGFLGEVVGTLEWFDSAAATF
                     VVKDREGRLVRVPEKGVRHGKPV"
     gene            2420..2680
                     /locus_tag="VM638_05790"
     CDS             2420..2680
                     /locus_tag="VM638_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11970.1"
                     /translation="MERFLVVYDTGQGGAWAYLRARSRAEIYMRFPELEVVDRPPGWM
                     SPEVLERIEREMTADVDEDRDALIARLRARPAEGPVPGRRPR"
     gene            complement(2685..>3693)
                     /locus_tag="VM638_05795"
     CDS             complement(2685..>3693)
                     /locus_tag="VM638_05795"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11971.1"
                     /translation="SMTQARSITATFTAAHTLTVSQTGSGAGGVTSSPAGIDCGSDCS
                     ENFLHGTAVTLTPLADEHSDFSGWTGCTSVTGDTCTVELTSSTTVTASFTQRTYALQV
                     DRSGSGSGSVGSSDAGIMCGADCSHTFGSGTSVTLTATPATGSVFWSWTGCDSVTGTQ
                     CTVTLTSERTVTATFKKQYRLEVTTSGSGTVTSSSSGIDCPSDCAEDVVDGTTVTLTA
                     TPASGSIFTGWSGGGCSGTGTCVITVGGDTTVAASFATAFTLTVNRMGPGIVSSSPGG
                     ISCGADCVEVYQSGTSVTLTATSTGGTGTITWGGACLGTIGSQCTVTMSANRSVSVTF
                     S"
BASE COUNT          511 a         1265 c         1358 g          559 t
ORIGIN      
        1 gagacggcgg agcatcccgg cgtcatggac gccgagcagc tcgacctggc ggggttctgc
       61 gtcggcacgg tcgcccgcga cgcgacgctc gggccgcacc gggtcggcga aggtgatctg
      121 ctcgtcggtc ttccctcgac ggggccccac gcgaacgggt attcgctgat ccggcggctc
      181 gttgagggcc tcgacctgga ggcgcctgcc ccctggagcg gccgagctct ggcggaggag
      241 ctcctcgagc caaccgcgat ctacgtccgg cccgtcctcg aggccgtgcg cgcgggcctc
      301 gtttccgccg cggcgcacat caccgggggt ggcctggtcg agaacctgcc acgcgccctg
      361 ccgcgcggcc tgggcgccga cctcgatcga gcctcctggg acgtgccgga ggtgttcaag
      421 ttcctccagg aacggggcga catacccgag gaggacatgg cgcgcacctt caacctcggg
      481 ctggggatga tcctggtcgt ccccgacgag cgggtcttgg acccgctctc atcagctggg
      541 ctggaggcgt cggtcgtggg gcgcgtggcg gccggggaag gggtccgctg gtgaagcgcg
      601 gcgtgctcac gttgcgggcc acgcgcggtc cggatctcca ggaggtccgc agccgtcgtt
      661 cgcagctgca ccggctgctg ctggtgatcg tcgtcctgct ggtggccgcg gtcgtgctgt
      721 tctcgatcgc ggcggagcag ctcctccatc gggccctccc gttcgtgggg atcgtcgcga
      781 cccgcgccgg attcgcggcc gtgatgctcc tgttcgcctt ctacgtctgg cgccgggagc
      841 gggcgctctc ccgcgccgaa cgtgcgctcg tcgagggacg ggtgctcgca gcggcgctgt
      901 cgaacaggat gcacgagctc tcggccgtgt cggaagccgg gcgggcggtc tccgcggtgc
      961 tgtcggagaa gcaggtgatg caggtcatcc tcgattccgt ccgggatctc ctcgacgcga
     1021 cggaagcatc cgtgatgctg ctcgacccgg agacgaacgt gctccggctg gccgccggct
     1081 gggggatcga cgaggaggtc acgcgcggaa ccgcggtcat ggtcggggag ggcatcgcgg
     1141 gctgggtggc ggaatcgctc gagccggtga tcctcaatgg tgacgtgcgc gacccgcgct
     1201 tccgcgggtt cgtcccgaag gaccggccgg ttcgctcggc catgtccgcg ccgctgcgcg
     1261 ccgggagcac cacggtcggc gtgatcaacg tgagcgtctc cggcggcggg cgcgagtaca
     1321 ccgagcacga cctccgggcc ctcacggact tcgccgagca cgccggcgcc gcgatcgcga
     1381 acgcgcggct cttcgagcgc gagcgccagg catcctcgga gctcgcggcg ctcgagcagc
     1441 agcggcgcga cttcctggcg acggtcacgc acgacctcaa gaccccgctc acctcgatcc
     1501 tcgggtacgt gaagctcctg cggcggatgg acgattcgct tgactccgag caggcgcagg
     1561 ccttcacctc ggtgatcgag cggcaggggc agcggatcct cgagatggtc gagcagctcg
     1621 tcgaggcgag caagctcgaa gccggcgctc cggccctcgc tcgcgaacgc ctcgacctgg
     1681 gccggatcat cgacgagcag gtcctcgccg tggggggact gctcggaggc cggagcgtgg
     1741 acgtggggct gccggacgcg ctcccgccga tgtacggcga tccgcaggcg gtcgaacaca
     1801 tcctgatgaa cctgctcgag aacgccgtga agtacacgcc ggacgggtcc tcgatcaccg
     1861 tcgaggtcac accggcggac ggcgaggtgc aggtgagcgt gaccgacgac gggccgggta
     1921 tccccgagga cgagctcccg cgggtcttcg accgctaccg gcaggcgacc gagcggacgg
     1981 agcgcgggag cgtcgggctc ggcctctaca tcgtccgcaa cctcgccgag gcccacggcg
     2041 gccgggcgtg ggcagagaac gcgccgggga agggagcgag gatcacgttc acccttccgg
     2101 tccgtgccga gcggaagcct gccgaggtct gagggctaga cgggcttccc gtgccggacg
     2161 cctttctccg gcacgcggac gaggcgcccc tctcggtcct tcaccacgaa ggtcgccgcc
     2221 gcggaatcga accactcgag cgtcccgacg acctcgccga ggaagccgtt cggcagctcg
     2281 tactggaacg tgacgcgccg cccgacgtgg tcgggggtca cgaggttctt gccgcgcatg
     2341 gccccaccgt agagcctggg gagccacggc gacaacccgg gggtgcgtgc ccccgggagc
     2401 cgggggaagg atgcctcgga tggagcggtt cctcgtggtc tacgacaccg gccagggcgg
     2461 cgcgtgggcc tacctccgtg cccgctcgcg cgcggagatc tacatgcggt tcccggagct
     2521 cgaggtcgtg gaccggccac cggggtggat gtcccccgag gttctcgagc gcatcgagcg
     2581 cgagatgacg gccgacgtcg atgaggatcg ggacgcgctg atcgcgcgcc tgcgggcacg
     2641 gcctgcggaa ggcccggtgc ccggacgacg tccgcgctga tcgctcagct gaacgtcacg
     2701 gacacgctgc ggttggcgct catcgtcacg gtgcactgcg agccgatggt gcccagacag
     2761 gcaccgcccc aggtgatcgt tccggtgccc ccggtggagg tcgcggtgag ggtgacgctc
     2821 gtgcccgact ggtagacctc gacgcagtcg gcgccgcagc tgatgccgcc cgggctgctt
     2881 gatacgatcc ctggccccat gcggttcacg gtgagggtga acgccgtcgc gaagctcgcc
     2941 gcgaccgtgg tgtccccgcc gacggtgatg acgcaggttc cggttccgct gcagccgccg
     3001 ccgctccacc cggtgaagat ggacccgctc gcgggcgtcg cggtcagcgt cacggtcgtg
     3061 ccatcgacga cgtcctcggc gcagtcgctc gggcagtcga tgccgctcga agagctcgtg
     3121 acggtcccgg atccggacgt cgtcacctcg agccggtact gcttcttgaa cgtggccgtg
     3181 acggtgcgct ccgacgtgag cgtgacggtg cactgcgtcc ccgtgacgga atcgcagccg
     3241 gtccacgacc agaacaccga gccggtcgcc ggcgtggccg tcagggtgac ggatgttccg
     3301 gacccgaagg tgtggctgca atccgcccca cacatgatcc cggcatccga gctgccgacg
     3361 cttccggacc cgctcccgct ccggtccacc tggagggcat aggtccgctg cgtgaaggag
     3421 gcggtgaccg tcgtgctcga tgtgagctcc acggtgcagg tgtcgccggt gacggacgtg
     3481 cacccggtcc atccggagaa gtcggagtgc tcgtcggcca gcggcgtgag ggtgaccgcc
     3541 gtgccatgga ggaagttctc ggagcagtcg ctgccgcagt cgatcccagc cggcgagctc
     3601 gtcacacccc cggcccccga gcccgtctgc gaaaccgtga gcgtgtgcgc cgcggtgaac
     3661 gtcgccgtga tgctccgcgc ctgcgtcatg gag
//
LOCUS       DATLGG010000205        18905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_228668, whole genome shotgun sequence.
ACCESSION   DATLGG010000205 DATLGG010000000
VERSION     DATLGG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228668"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VM638_05800"
     CDS             complement(<1..581)
                     /locus_tag="VM638_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVM11972.1"
                     /translation="MRFALPERLSLQKIIQLILAIVLVIIVLVSSSKTLDTGRFTGAQ
                     WRSLVVNGIALGSLYALIAMGYTLVYGILFIINFAHGEVFMAGGFTAFFLARTLNNAG
                     FLNENPLVSLILLFAVGMLVSMLIAVTLERVAYRRLRGAPRLVPLITAVGASLFLQNT
                     FRGLYGAQVRSYPEIEMLSGSVSIFGMPVGKLSL"
     gene            complement(752..2035)
                     /locus_tag="VM638_05805"
     CDS             complement(752..2035)
                     /locus_tag="VM638_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVM11973.1"
                     /translation="MRRSKWFSLLALLFALSLFTAACADDGGGDTTGENGGGDAQAAC
                     DEDEFGCVEIAEGSPLTLGTLLVITGENASLGLDSQAGVELAADYYADQSFDGSPGEV
                     LGHTIEFDHQDDGCSPEGGTTGARALSSNESIVAVIGTSCSSAGEPASQILSDAGIVL
                     ISPSNTAPSLTDPETHQPFYFRTAHNDKIQGSAMAQFVAEELGHTTAATVHDGSPYAE
                     QLQQVFADTFSGEYGGEITSQEAVQVGQKDMTPVLTNIASGSPEFLYFPVFVAEGGLL
                     TAQARETSGLENTDLGGADGMLTPDWIDAAGAENAEGAYLSGPDLEFSGDFYDAEFLP
                     AYEETVGSEPTSVFHAHAFDATNMVLQAIEEVAIQEGGTTYIPRTALRDALVGATHDG
                     ITGALECDENGDCNQDATISVSVVENGKFKRIWPE"
     gene            complement(2214..2822)
                     /locus_tag="VM638_05810"
     CDS             complement(2214..2822)
                     /locus_tag="VM638_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM11974.1"
                     /translation="MGDGSGRKVLIAEDEAIIRLDLKEMLEEEGFDVVGEAADGDAAV
                     RLTRDRSPDLVIMDVKMPGRDGLSAAEAIAEDDLAAVLILTAFSQRDLVRRAADAGAM
                     GYLVKPFQKSDLLPAIEIALARRAELVAVRRESESLGEQLETRKLVDRAKGRLMDEGG
                     LSEADAFRHIQKQAMDRRLSMRAVAEEVLQERASGPEPLRHE"
     gene            complement(2827..3105)
                     /locus_tag="VM638_05815"
     CDS             complement(2827..3105)
                     /locus_tag="VM638_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11975.1"
                     /translation="MHIEKRLSQVGDRLARVREELRILEEQLLFQMDVLEEAKTRMLV
                     SENPVADREFRIARDDHDRLVRQREGMSEEIADLLREQDRLLDRMLNA"
     gene            complement(3159..3587)
                     /locus_tag="VM638_05820"
     CDS             complement(3159..3587)
                     /locus_tag="VM638_05820"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HVM11976.1"
                     /translation="MDLGHLGVLNWLAIAVATLAYFALGGVWFLPGMFGKAWSRSMGW
                     DMPEGAEGPGAAIYIGPLLTSLVASIALALLAEATRADGAGDGLILGLVVGLGIAGSV
                     LLLTAVFDPKKPEPRTWFAITGGYHIAGLTLASVIISAWR"
     gene            complement(3617..3700)
                     /locus_tag="VM638_05825"
     tRNA            complement(3617..3700)
                     /locus_tag="VM638_05825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3664..3666),aa:Leu,seq:caa)
     gene            complement(3803..4651)
                     /locus_tag="VM638_05830"
     CDS             complement(3803..4651)
                     /locus_tag="VM638_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11977.1"
                     /translation="MSELRELTDRDIERLLAGDALERADLTEVASHLRNLRAFSARAP
                     GVEVANRHVVAAASAARAASGDAVLEGAASPAPARRGFLERTRLAVARLATGAVALSG
                     ATAGLAYAGVDLPGSAAEQAFDKVGIELPNQAPDTEAADVADRVHAVIEAWPQQERGC
                     AFGQAVAAAARDDDVQTCRSDASKAGAEAAKGKAEGKAKGPEKARGGRATGTAKSARG
                     RAEAAERSDGPAAPEQSSGGRATAEERSSSAGTTGEEHSSAGRAAAEEKSGGGQANKP
                     ENTGKP"
     gene            complement(4678..5235)
                     /locus_tag="VM638_05835"
     CDS             complement(4678..5235)
                     /locus_tag="VM638_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM11978.1"
                     /translation="MTLGQSFTEVLAAARTGAEWAWRALYDDLAPPVLGYLRGRGVPD
                     PEDALGEVFLQVVGGLGRFTGDESSFRSWVLTIAHRRAVDAHRRAARRPAVPIAADLL
                     EAAGPQGDTEEEGLSRIEAARALEAIRSLSPDQQDVLLLRLMGGLTTEEIARIVGKKP
                     GAVKALQRRGLATLRRKISGEGVSE"
     gene            complement(5435..6226)
                     /locus_tag="VM638_05840"
     CDS             complement(5435..6226)
                     /locus_tag="VM638_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11979.1"
                     /translation="MAGDSSGAGWRLPALLWVTVAGAILASCSPARTPVPAPAPSGPP
                     EVEAAAALGHARELARELGARAPGSQEEQAASVYVTAHLQQAGYAVFLDGVPVEDLVR
                     STNVVARPPAGGAPEVVVVAPYGTPPEESDGGAAAGLVLELARALRVRSPEHAAGFAL
                     LGAEHAAAGPLGSRRLAEDLAEDAPGALVVVVGDVESDAGCLAAGGAEGLVGRLTPTC
                     PETPTGVADASRRVFAAAGLETAAVSGDLDRVATALLRLLEGTEA"
     gene            6225..6491
                     /locus_tag="VM638_05845"
     CDS             6225..6491
                     /locus_tag="VM638_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVM11980.1"
                     /translation="MTGTPRPIAWLALDEGTPVWGTDGSELGSVGTVVGDEQKDIFHG
                     ITIRSGLFSDERLVPADRVEEVLTDRVVVRVSEEEMDALEVYRP"
     gene            complement(6518..6841)
                     /gene="hisI"
                     /locus_tag="VM638_05850"
                     /pseudo
     CDS             complement(6518..6841)
                     /gene="hisI"
                     /locus_tag="VM638_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375790.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            complement(6838..7980)
                     /locus_tag="VM638_05855"
     CDS             complement(6838..7980)
                     /locus_tag="VM638_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVM11981.1"
                     /translation="MTTFLLYDDALRSPELRHEISEPVMDPMVFIEHDDRRVLIGSVL
                     EAPIFDDRPEIVDEFWLQDSLGSEDLIKDASFPVDLVDLEVARRALERLGARTVVVPP
                     TFRVKAADYLRERGVEVVVDAEAWMLRRRRKSPAELEGIERAQRATETAMLAAQRMLR
                     EAEPTSDGRLRFEGEILTAEWIRQVMQQELGTQGAECEDIIVHSGDACLNGHDIGSGP
                     ILPDQTVIIDCFPKDRRSGAYTDMTRTFVPGEPSEEIKRIHADCCRALEIAYEHLEPG
                     RDDAHRAVVDFFHERGYPTREHHEGDEPLGVGFFHSLGHGVGLEVHERPWVGRRPDAL
                     ADGDVVAIEPGLYFPGVGGVRLEDTVVVTDDGPHRFTDPFPYDLEV"
     gene            complement(7977..8753)
                     /gene="hisF"
                     /locus_tag="VM638_05860"
     CDS             complement(7977..8753)
                     /gene="hisF"
                     /locus_tag="VM638_05860"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HVM11982.1"
                     /translation="MTRLAKRIVPCLDVDGGRVVKGVRFTGLRDAGDPVELAARYDAE
                     GADELVFLDITASHERRDIILEVAARTAESVFIPLTVGGGIRSVDDVRRLLRAGADKV
                     AINTAAVERPEVLKEAAGEFGRQAIVAAIDARARADGWEVYVQGGRVATDLDAVEWAR
                     RAAALGAGEILLTSMDRDGTTDGYDLALTRAVVDTVEVPVIASGGAGRPEDLADAVDP
                     ARGAAEAALAASVFHFGTFSIGDAKAALDRRGLTVRITRP"
     gene            complement(8750..9520)
                     /gene="hisA"
                     /locus_tag="VM638_05865"
     CDS             complement(8750..9520)
                     /gene="hisA"
                     /locus_tag="VM638_05865"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HVM11983.1"
                     /translation="MSFTVFPAVDISDGRCVRLLQGRFGTETVYSDDPVKVAIGLSKA
                     GARWLHIVDLDAAKTGEPVNRELVLEVVRRASCPVQAGGGIRSLDDVTEILAAGANRA
                     VLGTAALEDPVEMKRACARYGERIAVSLDARGDELASHGWTVGSGTSVDEAIATFDDA
                     GAAAFIYTDVTRDGMMAGPNLDALARVSSLTSRPVVASGGISSLADLTSVARMYPQNV
                     RGAIVGRALYEGKFSVGEAQYAADEAAAGRAEPPLVES"
     gene            complement(9517..10095)
                     /gene="hisH"
                     /locus_tag="VM638_05870"
     CDS             complement(9517..10095)
                     /gene="hisH"
                     /locus_tag="VM638_05870"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HVM11984.1"
                     /translation="MSRRVAVLDHNMGNLRSVAKALEAAGARVAVTAEPDEIQACDAL
                     CVPGQGIFGRCMSALVSGGTGDLVRDWVAGDRPFLGICLGMQVLFEASEESPEPGLGI
                     LPGGCRRLRGPVTIPHIGWNEVDSSEGRTYYYFDHSFAAHPAEAEVVDGWCEHGERFA
                     ASVRRDRLLAVQFHPEKSGAAGLRLLKGWVDD"
     gene            complement(10092..10679)
                     /gene="hisB"
                     /locus_tag="VM638_05875"
     CDS             complement(10092..10679)
                     /gene="hisB"
                     /locus_tag="VM638_05875"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HVM11985.1"
                     /translation="MTRRARVERRTNETSVVVEVDLDGGDVRAATGIPFFDHMLDQLG
                     RHGRLGLSIVAEGDLEIDAHHTVEDCGIVVGKAIAEALGDKAGIRRYGDALVPMEESL
                     AQVALDLSGRPLLVYDADLPAEAIGAFDVVLVEEFLLALVRAAGLTLHVRLLRAKNAH
                     HAVEAIFKALAVALGEALEIDPRGRGVVPSTKGLL"
     gene            complement(10676..11734)
                     /gene="hisC"
                     /locus_tag="VM638_05880"
     CDS             complement(10676..11734)
                     /gene="hisC"
                     /locus_tag="VM638_05880"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HVM11986.1"
                     /translation="MRRPSVRDDLATVAPYISPQQQARYRMNVNESPYSPPPAVLEEA
                     FAEVRQREVNRYPERDAAPLQEAVAAHLGVAPESIWLANGSNEVFQHLFLAFGGSGRK
                     TLVFEPTYSLHTLIPRITGTAVATRPRTDGFGIDPDEARRAVAEERPDLVLLCSPNNP
                     TGNLAPPDLVRATAEGASLVVVDEAYVEFARPGSSVLDLVEELPNVVVVRTFSKAWRL
                     AGVRLGYLVGAPSIVRDLLRVRLPYHLSVFTQAMGTAALRHAADALEHVGAIVAERER
                     MGAGLSALGLRVHPSEANFVLFDVDDPGRVWRDLADRGVLVRRYDGAPRLERSLRVTA
                     GLPDETDAFLAAMDEVVG"
     gene            complement(11743..13035)
                     /gene="hisD"
                     /locus_tag="VM638_05885"
     CDS             complement(11743..13035)
                     /gene="hisD"
                     /locus_tag="VM638_05885"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HVM11987.1"
                     /translation="MLEIIDGRDARTPVHIPRPRAARGAAEPDGTVRAIVEAVRSRGD
                     EALIELTDRHDRVRLTPDRIRVEPEIIEKAPSLVPPEFVDALEVMAARLRRTCERQMP
                     REWIEDDGDEFSGELVRPLRRAGIYAPGGRYPYPSSVVMAAVPAQVAGVTGIAVCSPP
                     GLGGEIPDVILTACRVLEIDEVYRLGGAQAIAALAYGTQSVRPVEKIVGPGNIFVTLA
                     KRQVSGWVGIDGDAGPTEIAIVADATADPQETAADLVAQAEHGPLGTHALITWEASLA
                     EAVLDALEPLVMAHPHPEEVENALIEGGRAVLVHDLDHALETANAFAPEHLQLVFPGA
                     REALGKVRNAGSVFVGRYSPVPVGDYVGGTNHVLPTGASARWESGLAVRDFVKHIYVS
                     GYERSALARLAPHVEALADAEGLHGHARAVELRLSDEL"
     gene            complement(13099..13332)
                     /locus_tag="VM638_05890"
     CDS             complement(13099..13332)
                     /locus_tag="VM638_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11988.1"
                     /translation="MPCYRCGRIQTDPARGASPWARGVVGDEQVLVCPECQTAEPSWP
                     DDLARCPSCGSTRLGIVMGSVVCRACGHDRPLD"
     gene            13378..14163
                     /locus_tag="VM638_05895"
     CDS             13378..14163
                     /locus_tag="VM638_05895"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HVM11989.1"
                     /translation="MDERGMAAAVEDAAARVGSLLRSVKDPQAPAVGTWKIRDVAVHL
                     AGVFSAYPGYLDRSTPLFEHPMDVVAHNEAVVSSDETTDLGAVADRIEASATGLAGAL
                     TAGAATGAVPWHGGSTIPGASIPAIAVTEAFVHGHDIAAAEGRRCQFPRRPLAFGLRG
                     LLDVLPLYVDRERAADAVVCFDVRIRGGERAGLVFHRGTLTVHEGGAPSRPDCTLWVD
                     PGGMLLVGYERVSPLAAVARGWVAAWGRKPWLGLALPKLIRQP"
     gene            complement(14170..17607)
                     /gene="dnaE"
                     /locus_tag="VM638_05900"
     CDS             complement(14170..17607)
                     /gene="dnaE"
                     /locus_tag="VM638_05900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVM11990.1"
                     /translation="MSFVHLHAHTEYSMLDGASRISEMFEAAANAGMPACAITDHGVM
                     YGALDFYRSGRSHGVKPILGMEGYLFGGNRREKPPQRENGERTYHLTLLAMDQTGYGN
                     LVKISSRAWLEGFHYQPRSDWELLTEHAEGVICLSGCLSAEIPRAIVAGDPAGARRKV
                     AQYRELFGDRFYLELQDHGLAQQGPLNDELVAIGREMGIPWVCTNDSHYTHKDDAAGH
                     EVLLCINTGSELSDPNRFKFDSEEFYLKTPEEMQRAFARWPGACETTLDIAERCNVEM
                     TFDKLLLPRYDVPAGETLDSYLERLVLDGCRRRYGEPLPAEVSERARYELRVIADMGF
                     AGYFLVVQDFVNWAKRQGIRVGPGRGSAAGSIVSYAIGITELDPIKYDLMFERFLNPK
                     RVAMPDIDIDFDDRRRGDVIRYVQDKYGDDRVAQIVTYGTIKGKQAIKDAARVLGFPY
                     AEGDRLTKMYPPLVMGRDSGLRSALETSPELAAAYQGGGAAKEIIDTALQVENLKRSA
                     GIHAAAVVIGRDPLVEHVPLRRGEHGEIVTQFDMNGVEELGLLKMDFLGLRNLSVIEL
                     TLDYVEANRGTRPDVDDLPLDDPKVYEILRRGDSDGVFQLESEGMRRLLVQLKPDRFE
                     QIMALIALYRPGPMDEIPKYLERKHDPSKVEYLHPALERITAETYGVLVYQEQVVQLL
                     QILAGYSPGEADMVRKAIGKKNREIMAREEPRFISGCTASGMSEADAKRLWALIQPFA
                     DYSFNRAHSACYAYVAYQTAWLKAHYPVEYMAALLTSVKDRKDDKPKYLHMARKMGIP
                     VLIPDVNSSEKDFTPTGDSIRFGLSAVRGLGDGVVEKIIDARLRKGAFESFHDFCRKV
                     DYICLNKKTVESLIHAGAFESLGHTRKGLAETFERLCADIVDRRKQEEAGQFSLFGAA
                     CGEGGAHQDTPIPRDEYPKELLLAHEKEMLGLYVSDHPLLGVEGLLSRMTDGPISALE
                     GRSQGESVTIGGLVAGYQKRVTKSGGVMNILTVEDLSGATVEVLCFPKTVEQFGAILR
                     PDAILLIRGRLDRDVRDDSIKLIAQDVKEPKLGDEGPLEVTLPSDACSETLIAKLKDV
                     LRAHPGNTPVFLHLRARSGRTTTLKLGSEFWVSTSNGIHAELKALLGPGALDPGAAA"
     gene            complement(17802..18725)
                     /locus_tag="VM638_05905"
     CDS             complement(17802..18725)
                     /locus_tag="VM638_05905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVM11991.1"
                     /translation="MTGTGVSFTVAPGEAGGRVDVVVAGRAGVTRTLVQRAVREGLVT
                     VGGVAVRPSHRLEEGDVVAGTIPLPESGPPEAEDIPVGVRYEDERVLVISKPPGLVTH
                     PARGHEAGTLVNALLGLGVPLSGAGSTRPGIVHRLDKDTSGLLLVAKDDGAAAVLVEA
                     IRQREVERRYLALVRGRPPAASGTIEAPVGRHPVRRRRMAVVAEGRPSVTHYRAVDAT
                     DETALLDVALETGRTHQIRVHLSHLGFPILGDAVYGGKGDLARSLGLERPFLHAYLLR
                     FPHPDGGEVEVTDPLPADLADVARRAGVSPP"
     gene            complement(18722..>18905)
                     /locus_tag="VM638_05910"
     CDS             complement(18722..>18905)
                     /locus_tag="VM638_05910"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVM11992.1"
                     /translation="LVLGGGLGNVSDRIFRATGGRVVDFVDLHVWPIFNVADACITVG
                     VLVLLVVGWRAAPAER"
BASE COUNT         2842 a         6786 c         6434 g         2843 t
ORIGIN      
        1 aggctgagct tgccgacggg cattccgaag atcgagacgg agccggagag catctcgatc
       61 tccgggtacg accgcacctg cgcgccgtag agcccacgga aggtgttctg caggaacagc
      121 gacgcaccca cggccgtgat cagagggacc agtcgcgggg cgccgcgcaa cctccgatag
      181 gcgacgcgtt ccaacgtcac cgcgatgagc atggacacga gcatgccgac cgcgaagaga
      241 aggatgagag ataccaacgg gttctcgttg aggaacccgg cgttgttcag ggtgcgggcg
      301 aggaagaacg cggtaaatcc acccgccatg aagacctcgc cgtgcgcgaa gttgatgatg
      361 aacaggatgc cgtagacgag cgtgtagccc atggcgatca gggcgtaaag gctgcctaga
      421 gcgatgccgt tcaccacgag ggaacgccac tgcgcgccgg tgaaacggcc ggtgtcgagc
      481 gtcttcgacg agctcacgag cacgatgatc accaggacga tcgcgaggat cagctggatg
      541 atcttctgca gcgagagccg ctcaggtaag gcaaaccgca tgcatcatcc tctccgccgt
      601 aaagaatgac ccgggcgcct tcgatcgtgc aggggcgcgc attctgctgg aaacctaccg
      661 cggcttgccg ccgcggttcc ggacgaacat gcgggtgggc ccgtcgccga gcccacccgc
      721 ggtcgttcgt ttggtgcgaa ctacctccgg cctattccgg ccagatgcgc ttgaacttgc
      781 cgttctcgac cacgctcacc gagatcgtgg cgtcctggtt gcagtcgccg ttctcgtcgc
      841 actcgagcgc gccggtgatg ccgtcgtggg ttgcccccac cagggcgtcc cgcagagcgg
      901 tccgcgggat gtaggtggtg ccaccctcct ggatcgccac ctcctcgatc gcctggagca
      961 ccatgttcgt ggcgtcgaag gcgtgggcgt ggaacacgga ggtcggctcg ctgcctactg
     1021 tctcctcgta cgccgggagg aactcggcgt cgtagaagtc gccggagaac tcgaggtcgg
     1081 gtccggagag gtaggcaccc tcggcgttct ccgcgcccgc cgcgtcgatc cagtccggcg
     1141 tcagcatccc gtcggcaccg ccgagatcgg tgttctcgag accggaggtc tcacgcgcct
     1201 gggcggtgag cagaccaccc tcggccacga acacagggaa gtagaggaac tcggggctgc
     1261 ccgaagcgat gttggtgagg accggcgtca tgtccttctg cccgacctgc acggcttcct
     1321 ggctggtgat ctcaccgccg tactcgccgc tgaacgtgtc ggcgaagacc tgctgcagct
     1381 gctccgcgta cggcgatccg tcgtgaacgg tcgcggcggt cgtgtgaccg agttcctcgg
     1441 ctacgaactg cgccatggcc gagccctgga tcttgtcgtt gtgcgcggta cggaagtaga
     1501 acggctggtg cgtctccggg tcggtcagcg acggcgccgt gttcgacggg gagatcagga
     1561 cgatcccggc gtcggacagg atctgcgaag ccggctcacc cgcgctggag cacgaggtgc
     1621 cgatgaccgc gacgatcgac tcgttcgacg aaagcgcgcg ggcaccggtc gtgccgccct
     1681 cgggcgagca gccgtcgtcc tggtgatcga actcgatcgt gtgacccagc acctcgcccg
     1741 gcgagccgtc gaaggactgg tcggcgtagt agtcggccgc cagctcgacg ccggcctggc
     1801 tgtcgaggcc gagcgaagcg ttctcgcccg tgatcacgag cagcgtgccg agcgtgaggg
     1861 gcgagccctc cgctatctcg acgcagccga actcatcttc gtcgcacgcg gcctgggcgt
     1921 caccgccgcc gttctccccg gtggtgtccc cgccgccgtc gtccgcgcac gccgccgtga
     1981 acagcgacag agcgaagagc agtgcgagaa gcgagaacca cttcgacctt ctcatccaag
     2041 tcccccttac tcctgacaac agacccggtt tccccgcagc gtggacgcga ccgggatcag
     2101 ccggagcccg cccacaacgt ggaaaacggc gctagtttac cgacccccgc ctggatgtcc
     2161 aacgtcatga ggcaagaact tcctttttct tgtccaaaaa ggcgccgcgg ctactactcg
     2221 tgtcggagtg gctcgggtcc cgacgcccgt tcctgcagca cctcttcggc gacggcgcgc
     2281 atcgagaggc ggcggtccat cgcctgcttc tggatgtgcc ggaaggcatc ggcctcgctc
     2341 aggccgccct cgtccatgag cctccctttg gcccggtcga cgagcttccg ggtctcgagc
     2401 tgctccccga gcgattcgct ctcgcgcctc accgccacca gctcggcgcg ccgcgccagc
     2461 gcgatctcta tcgccggcag caggtcggac ttctggaacg gcttgacgag gtagcccatc
     2521 gcgccggcat cggcggcgcg ccgcacgagg tcgcgctgtg agaaggcggt caggatcaga
     2581 acggccgcga ggtcgtcctc cgcgatcgcc tccgccgccg agagtccgtc gcggcccggc
     2641 atcttgacgt ccatgatcac gaggtcgggc gatctgtcac gggtgagccg cacggcggcg
     2701 tcgccgtcgg ccgcctcgcc caccacatcg aagccctctt cctcgagcat ctccttgagg
     2761 tcgaggcgga tgatcgcttc gtcctccgcg atcaggacct tgcgcccgga gccgtctccc
     2821 atcgcctcag gcgttcagca tgcggtcgag aaggcggtcc tgctcgcgca gcagatcggc
     2881 gatctcctcc gacatcccct cccgctgccg gaccagccgg tcgtgatcgt cgcgcgcgat
     2941 ccggaactcc cggtcggcga cggggttctc ggacaccaac atccgcgttt tcgcctcttc
     3001 gaggacgtcc atctggaaca agagctgctc ctcgaggatg cggagctcct cacggaccct
     3061 cgccagccgg tcgcccacct gggacaggcg cttctcgatg tgcatgggcg cagaagtata
     3121 ggtgacgaag ggggcggatc cgcggttccg gcggcgcctc agcgccaggc ggagatgatc
     3181 accgaagcca gcgtgagccc cgcgatgtga tagccgccgg tgatggcgaa ccacgtcctg
     3241 ggctccggct tcttcgggtc gaacaccgcc gtgagcaaca acacgctgcc ggcgatccca
     3301 agccccacga cgaggcccag gatcagcccg tcccccgcgc cgtccgcacg cgtcgcctcc
     3361 gccaggagtg cgagcgcgat cgaggcgacg agggaggtca ggagcgggcc gatatagatg
     3421 gcggcgcccg gcccctcggc gccctccggc atgtcccagc ccatggagcg gctccacgct
     3481 ttcccgaaca tgccgggcag gaaccagacg ccgccgaggg cgaagtacgc cagcgtcgcg
     3541 accgcgatcg cgagccagtt gagaaccccc aggtgcccta gatccacgtc gcaaatatac
     3601 cgacaggtcc ggtgcgtgcc ccgggtggga ttcgaaccca cacggcccga aggccaatgg
     3661 attttgagtc catcgagtat gccagttcct ccaccggggc cggggggagt ctagtcgttc
     3721 gctcgttcga tcccggagac gaaacgaccc cgcccgaggg gcggggtcgt tcgggcacgg
     3781 taggtgaaac gcctgtctct tgttagggct tgccggtgtt ctccggcttg ttcgcctgac
     3841 ccccgccgct cttctcctcc gcggcggccc ggccggcgga agagtgctcc tcgccggtgg
     3901 tgcctgcaga cgaggaacgc tcctcagccg ttgctcgacc gccggaggac tgctcgggcg
     3961 ctgccggtcc gtcggatctt tcggcggcct cggcgcgccc gcgggccgac ttcgcggttc
     4021 cagtggcacg accccctcgc gccttctcgg gacccttcgc tttgccctcg gccttgccct
     4081 tcgccgcttc ggcgccggcc ttcgaggcgt cggagcgaca ggtctgcacg tcgtcgtcgc
     4141 gagcggcagc tgcgaccgcc tgtccgaagg cgcagccccg ctcttgctgc ggccacgcct
     4201 ctatcaccgc gtggactcga tcggcgacgt ccgccgcctc ggtgtccggc gcctggttgg
     4261 gcagctcgat accgaccttg tcgaacgctt gctccgccgc ggagccggga agatccacgc
     4321 cggcgtaagc caagcctgcg gtcgcccccg aaagcgcgac ggcgccggtt gccagccggg
     4381 ccaccgcgag tctcgtgcgc tcgaggaagc cgcggcgcgc cggtgccggc gacgccgccc
     4441 cctcgaggac ggcgtcgccg gacgcagcgc gggctgcgga ggccgccgcg acgacgtgac
     4501 ggttcgcgac ctcgaccccc ggagcccgcg cactgaatgc ccgcaggttc cgcaggtggg
     4561 acgcgacctc ggtcaggtcg gcgcgttcga gcgcgtctcc cgccagaagg cgctcgatgt
     4621 ctcggtccgt cagctcccgc agctcactca tctcgtgtcc gtaatcgccg cttcgcctca
     4681 ttcggatacg ccctcgcccg agatttttct ccgcagcgtc gcgagaccgc gccgctgcag
     4741 cgccttcacc gcacccggct tcttgccgac gatgcgtgcg atctcctcgg tcgtgaggcc
     4801 gcccatcaac cgcagcagga ggacgtcctg ctggtccggt gagagcgacc ggatcgcctc
     4861 gagcgcgcgc gccgcctcga tgcggctcaa gccctcctcc tcggtatcgc cctgggggcc
     4921 cgccgcctcg agcagatccg ccgctatcgg cacggcgggg cgtcgggcgg cgcggcgatg
     4981 cgcgtcgacc gcgcgacggt gggcgatcgt gagcacccac gagcggaagc tcgactcgtc
     5041 ccccgtgaac cggccgagac ctccgacgac ctgcaggaac acctcgccca gagcgtcttc
     5101 cgggtccggc accccccgac cccgcaggta gccgaggacg ggcggcgcga ggtcgtcgta
     5161 gagggcccgc caggcccatt ccgcgccggt ccgggcggcg gccagaacct cggtgaagga
     5221 ctgaccgagc gtcacgaccg ggagcctcct ccgggccgcc cgatgaggtg aggcgcgcgc
     5281 tgcgggatgg gcggcctagg gccgcggtgg gtgccccttc cggatcctga tctggcgcat
     5341 aggtccctcc cgcagaggat gctgtcacct cctacccctc tcggcgtgga tttgcagaac
     5401 ctgagccgca gagcgggagg tggaggaggc ggcgtcacgc ctcggtcccc tcgaggaggc
     5461 ggaggagcgc cgttgccacc cggtcgagat cgccggacac ggccgcggtc tccagccccg
     5521 cggcggcgaa gaccctccgg gacgcgtccg cgacgcccgt gggagtttcg gggcacgtag
     5581 gcgtcagacg cccgaccaac ccctcggcac cgcccgcggc cagacacccc gcgtccgact
     5641 cgacgtcgcc gacgaccacc accaacgccc ccggggcgtc ctccgcgagg tcctcggcga
     5701 gccgtcggct gccgagcggc cccgcggcgg catgctcggc tccaaggagg gcgaatcccg
     5761 ccgcgtgttc gggtgaccgg acccgcaggg cacgggcgag ctcgagcacg agccccgcgg
     5821 cggcgccgcc gtcgctttcc tcgggaggcg tcccgtacgg agcgacgacc accacctccg
     5881 gagcgcctcc ggccggcggg cgcgccacca cattggtgga gcggacgaga tcctcaaccg
     5941 ggacgccgtc gaggaaaacg gcgtagcccg cctgctgcag atgggcggtg acgtagacgc
     6001 tcgccgcctg ttcctcctgc gacccgggcg cccgcgcgcc gagctcccgt gcgagctccc
     6061 gcgcgtgacc cagggccgcg gcggcctcga cctcgggcgg tcccgaggga gccggagccg
     6121 ggacgggcgt gcgcgccggc gagcaggacg ccaggatcgc ccccgcgacc gtcacccaca
     6181 agagtgcggg gagccgccag ccggcgccgc tagaatcgcc ggccatgacc ggtaccccgc
     6241 gtcccatcgc ctggctcgcg ctcgacgaag gcacgcccgt gtggggcacg gacggcagcg
     6301 agctcgggtc ggtaggcacg gtcgtcggag acgagcagaa ggacatcttc cacggcataa
     6361 cgatcaggtc cgggctcttc tcggacgagc gtctcgtgcc ggcggaccgg gtggaggagg
     6421 tcctcacgga ccgggtcgtc gtgcgcgtct cggaggaaga gatggacgcg ctcgaggtct
     6481 atcggccctg accttccgcg gcggcgtccg gccggcggtc gaagctccgg tggaagcacg
     6541 tctgctcgcc ggtatgacag gcgggtccgg cggtctcgac cagcatgagg agcgcatcgg
     6601 cgtcgcagtc gtaacgcacc tcgcggacgt gctgcacgtt gccggaggtc gctcccttgt
     6661 tccagtactc ccggcggctc cggctccaga accaggtcgt gcgggaggag agcgtccgcc
     6721 gaacggcctc ggcgtcgcac cacgcgagca tcagcacgtc tccggacgac gcgtcctgca
     6781 cgatcgccgg gaccaatccc cgatcgtcga acttcaggcc ctccggctgc acccgcgtca
     6841 gacctcgagg tcgtacggga acggatcggt gaatcggtgc gggccgtcat cggtgaccac
     6901 cacggtgtcc tcgaggcgga cgcctccgac tcccgggaag tagaggccgg gctcgatcgc
     6961 caccacgtcg ccgtcggcca gcgcgtccgg ccgccgtccc acccatggcc gttcgtgcac
     7021 ctcgaggccg acgccgtgcc cgagcgagtg gaagaagccg acgccgaggg gctcatcgcc
     7081 ctcgtggtgc tcgcgcgtcg gataaccacg ctcgtggaag aagtcgacta cggcacggtg
     7141 cgcgtcgtcg cgccccggct ccaggtgctc gtaggcgatc tcgagcgcgc ggcagcaatc
     7201 cgcgtggatc ctcttgatct cttccgacgg ctcacccggc acgaaggtgc gcgtcatgtc
     7261 ggtgtacgcg ccgctgcgac ggtccttggg gaagcagtcg atgatgaccg tctggtccgg
     7321 gaggatcggg ccgctgccga tgtcgtgccc gttcagacac gcgtcgcccg aatgcacgat
     7381 gatgtcctca cactccgccc cttgggtgcc gagctcttgc tgcatcacct gccggatcca
     7441 ctccgccgtc aggatctcgc cttcgaagcg cagacgtccg tcggatgtcg gctctgcctc
     7501 gcgcagcatc cgctgcgccg ccagcatcgc cgtctccgtc gcgcgttgtg cacgctcgat
     7561 gccctccagc tcggcagggc tcttgcgccg ccgccggagc atccatgcct cggcatccac
     7621 caccacctct acgccccgct cgcgcaggta atcggcggct ttaacccgga aggtcggagg
     7681 caccacgacc gtgcgcgcgc cgaggcgctc gagcgctcgg cgcgccacct ccagatcgac
     7741 gagatccacc gggaacgacg cgtccttgat gaggtcctcg gagcccagcg aatcctgcag
     7801 ccagaactcg tcgacgatct ccggacggtc gtcgaagatc ggcgcctcga ggacgctccc
     7861 gatcaggacc cgccggtcgt cgtgctcgat gaagaccatc gggtccatca ccggctcgga
     7921 tatctcgtgg cgcagctccg ggctgcgaag cgcgtcgtcg tagagcagga aggtcgtcat
     7981 gggcgggtga tcctaacggt gaggccgcgc cgatcgagcg cggctttcgc atcgccgatc
     8041 gagaacgtgc cgaaatggaa gacgctcgcg gcgagcgcgg cctccgccgc gccgcgcgcg
     8101 ggatcgaccg cgtcggcgag gtcctcgggc cgccccgcgc ctcccgacgc gatcaccggc
     8161 acctcgaccg tatcgaccac ggctcgcgtc agcgcgaggt cgtacccgtc ggtggtgccg
     8221 tcgcggtcca tcgacgtgag gaggatctca ccggccccga gagccgcggc gcgccgcgcc
     8281 cactccactg cgtcgaggtc cgtcgcgact cgcccccctt gcacgtacac ctcccagccg
     8341 tcggcacggg cgcgggcgtc gatcgcggcg acgatcgcct ggcgaccgaa ctcccccgcc
     8401 gcctccttca acacctccgg tcgctcgacc gccgcggtgt tgatcgccac cttgtcggcg
     8461 ccggcgcgca ggagtcgccg cacgtcgtcg accgacctga tcccgccgcc gaccgtgaga
     8521 gggatgaaga ccgactccgc cgtccgcgcc gccacctcga ggatgatgtc ccgcctctcg
     8581 tgcgacgccg tgatgtcgag gaagacgagc tcgtccgccc cctccgcgtc gtagcgggcg
     8641 gccagctcga ccgggtctcc tgcatcccgc aggcccgtga agcgcacgcc cttgaccacc
     8701 cggccgccgt cgacgtcgag gcacgggacg atccttttgg cgagcctcgt cacgattcga
     8761 cgagcggggg ctcggcgcgc cccgcggcgg cctcgtccgc ggcgtactgc gcctcgccga
     8821 ccgagaactt cccctcgtag agcgcgcgcc cgacgatcgc gccgcggacg ttctgcggat
     8881 acatacgcgc cacggaggtg aggtcggcga gggacgagat cccgccggac gcaacgacgg
     8941 gccgcgacgt cagcgacgaa accctcgcga gagcgtcgag gttcggcccg gccatcatcc
     9001 cgtcccgggt tacgtccgtg tagatgaaag ccgccgcacc cgcgtcgtcg aaggtggcga
     9061 tcgcctcgtc gaccgatgta ccgctgccga ccgtccagcc gtggctcgcc agctcgtccc
     9121 cccgcgcgtc gagagagacc gcgatgcgct cgccgtagcg cgcgcacgcc cgcttcatct
     9181 cgaccggatc ctcgagggcg gcggttccca gcacggcacg gttggcgccc gccgccagga
     9241 tctcggtgac gtcgtcgagc gaccggatcc ccccgcccgc ctgcacggga cacgaggcgc
     9301 gtcgcacgac ctcgagcacg agctcgcggt tcaccggctc gcccgtcttc gcggcgtcga
     9361 gatcgacgat gtgcagccag cgcgcccccg ctttcgacag gccgatcgcc accttcaccg
     9421 ggtcgtccga gtacaccgtc tcggtcccga agcgtccctg gagcaagcgc acgcagcgac
     9481 cgtcggagat gtcgacggcc gggaagaccg tgaagctcaa tcgtccaccc aacctttcag
     9541 aagccgaagt ccggcggcgc cggacttctc cggatggaac tgcacggcca gcaggcggtc
     9601 gcggcgcacc gaggcggcga acctctcgcc gtgctcgcac cagccgtcga cgacctccgc
     9661 ctccgccgga tgggcggcga aagagtggtc gaagtagtaa taggtgcggc cttcggagga
     9721 gtccacctcg ttccacccga tgtgcgggat cgtcacgggg ccgcgtagtc ggcgacaccc
     9781 gccgggcagg atgcccagtc cgggctccgg tgactcctcg ctcgcctcga agaggacctg
     9841 catgccgagg cagattccca agaaggggcg gtcgcccgcc acccagtcgc gcaccagatc
     9901 tcccgtgccg ccggaaacga gcgcggacat gcacctaccg aagatcccct ggccggggac
     9961 gcagagcgcg tcgcaggcct ggatctcgtc gggctcggcg gtgacggcga cgcgcgcgcc
    10021 cgccgcctcg agcgccttcg ccacgctccg gagattcccc atgttgtggt ccagcacggc
    10081 gacgcggcgg ctcacagaag gcccttcgtc gacggcacga cgccgcggcc ccgcgggtcg
    10141 atctcgagcg cctcgcccag cgcgaccgcc agcgccttga agatggcttc gaccgcgtgg
    10201 tgagcgttct tcgcacgcaa gaggcggacg tgcagggtca gacccgcggc gcggacgagc
    10261 gcgagcagga actcctcgac cagcacgaca tcgaacgccc cgatcgcctc ggccggcaga
    10321 tcggcgtcgt agacgagcag cgggcgcccg gagaggtcga gggcgacctg cgcgagggac
    10381 tcctccatcg gaaccagagc gtcgccgtag cgacggatgc cggccttgtc gccgagggcc
    10441 tctgcgatcg ccttcccgac gacgatcccg cagtcctcca cggtgtgatg cgcatcgatc
    10501 tcgaggtcgc cctcggcgac gatcgaaaga ccgaggcgtc cgtgccgccc gagttggtcg
    10561 agcatgtggt cgaagaacgg gatacccgtc gcggcgcgta cgtccccccc gtcgagatcg
    10621 acctcgacca cgacgctcgt ctcgttcgtc cttcgttcga cgcgcgctcg ccgggtcatc
    10681 cgacgacctc gtccatcgcc gcgaggaagg catcggtctc gtccggcagg cccgccgtca
    10741 cacgcagcga gcgctcgagc cgcggcgcgc cgtcgtagcg ccgcaccagc acgccgcgat
    10801 cggcgagatc tcgccagacg cggcccgggt cgtcgacgtc gaagaggacg aagttcgcct
    10861 ccgaggggtg cacccgcagg ccgagggcgg agagacccgc ccccatccgt tctcgctcgg
    10921 ccacgatcgc tccgacgtgc tcgagcgcgt cggcggcgtg gcgtagagcg gcggtcccca
    10981 tcgcctgggt gaagaccgac aggtgatacg gcagacggac gcgcaggagg tcgcgcacga
    11041 tcgacggcgc gccgaccaga tatcccagcc gaacgcccgc gagccgccac gccttcgaga
    11101 acgtgcgaac gaccaccacg ttcgggagct cctcgaccag gtcgagcacg ctcgagcccg
    11161 gccgcgcgaa ctcgacgtac gcctcgtcga ccacgacgag gctcgcaccc tccgcggtag
    11221 ccctcaccag atcgggcggc gcgaggttgc cggtcgggtt gttgggcgag cacagcagaa
    11281 cgagatcggg acgctcctcc gccacggcgc gccgcgcctc gtccgggtcg atcccgaatc
    11341 cgtcggtgcg cggtctggtc gcgaccgccg tccccgtgat gcgcgggatc aaggtgtgca
    11401 gggagtaggt cggctcgaac accagcgtct tccggccgga gccgccgaac gcgaggaaga
    11461 ggtgctggaa cacctcgttc gagccgttgg ccagccagat cgactcgggc gcgaccccga
    11521 ggtgggcggc gaccgcctcc tgcagaggcg cggcgtctcg ctccggatag cggttcacct
    11581 cgcgttgtcg cacctccgcg aaggcctcct ccaggacggc gggcggcggc gaatacggcg
    11641 actcgttgac gttcatccgg tagcgagcct gctgctgcgg tgagatgtac ggggcgacgg
    11701 tcgcaaggtc gtcgcgcact gaaggacggc gcacggccac gctcacagct cgtcggagag
    11761 tcggagctct accgcgcggg catgcccgtg caggccctcg gcatccgcca gcgcctccac
    11821 gtgcggcgcg agacgagcca gggcgcttcg ctcgtacccc gagacgtaga tgtgcttcac
    11881 gaagtcccgc acagccagtc ccgactccca gcgcgccgag gctcccgtcg ggagcacgtg
    11941 gttggtcccg ccgacgtagt cccccaccgg gacgggcgag tagcggccga cgaagacgct
    12001 gccggcgttc ctcaccttgc cgagcgcctc ccgcgcgccg gggaagacga gctgcaggtg
    12061 ctccggggcg aacgcgttcg ccgtctcgag ggcgtggtcc agatcgtgga cgagcacggc
    12121 gcgccctccc tctatcagcg cgttctcgac ctcctcgggg tgcgggtgcg ccatcacgag
    12181 cggctcgagg gcgtcgagga ccgcctccgc gagggacgcc tcccacgtga tgagcgcgtg
    12241 cgtcccgagc gggccgtgct cggcctgcgc gacgaggtcg gccgccgtct cctgcgggtc
    12301 cgcggtggcg tcggcgacga tggcgatctc cgtgggcccc gcgtcgccgt ctatgcccac
    12361 ccatcccgac acctgccgct tggcgagcgt cacgaagatg ttccccggcc ccacgatctt
    12421 ctccacggga cgaaccgact gcgtcccgta cgccagcgcc gctatcgcct gcgcgccgcc
    12481 caaccggtac acctcgtcga tctccagcac ccggcaggcg gtcaggatga cgtcggggat
    12541 ctcgcctccg agccccggcg gcgagcacac cgcgatgccg gtcacgccgg cgacctgggc
    12601 cgggacggcc gccatcacga ccgacgacgg atagggatat cgaccccccg gcgcgtagat
    12661 cccggcgcgc cgcaacggtc gcacgagctc gcccgagaac tcatcgccgt cgtcctcgat
    12721 ccactctcgc ggcatctgcc gctcgcacgt acgccggagc cgggcggcca tgacctcgag
    12781 ggcgtccacg aactcaggcg gtacgagcga cggcgccttc tcgatgatct ccggctcgac
    12841 cctgatccgg tcgggcgtga ggcgaacgcg atcgtgccga tccgtcagct cgatgagcgc
    12901 ctcgtcgccg cgcgaccgca ccgcctcgac gatcgcgcgc accgtcccgt ccggctcggc
    12961 cgcgccgcgg gcggcacgcg gccgcgggat gtggaccggg gtccgggcgt cgcgcccgtc
    13021 gatgatctcg agcatggccc gagggtaatc gagggcgaca cagcgccagg gtcggccggg
    13081 agccgcgcct agacgcggtc agtcgagcgg acggtcgtgc ccgcaggcgc ggcagacgac
    13141 cgagcccatc acgattccga ggcgcgtgct gccgcaggac gggcagcgcg cgagatcgtc
    13201 gggccaggag ggctctgccg tctggcactc gggacagacg agcacctgct cgtccccgac
    13261 gacgccgcgc gcccagggcg acgcaccgcg cgcgggatcc gtctggatcc tcccgcagcg
    13321 gtagcacggc atctgactat tctgacgccg gcgggcggag ggagcggagg tggcgacgtg
    13381 gacgagcgcg ggatggcggc ggcggtcgag gacgcggccg cgcgtgtcgg gtcgctgctt
    13441 cgctcggtga aggatccgca ggcgccggcg gtcggcacct ggaagatccg cgacgtcgcc
    13501 gtacacctcg ccggggtctt ctccgcctac cccgggtatc tcgaccggag cactcccctc
    13561 ttcgagcatc cgatggacgt cgtcgcgcac aacgaagccg tcgtgagctc ggacgaaacg
    13621 accgacctgg gtgcggtagc ggaccggatc gaggcttcgg cgacgggtct cgccggagcg
    13681 ctcaccgcag gagcggcgac cggcgccgtc ccgtggcacg gaggctcgac gatccccggg
    13741 gcctctatcc cggcgatcgc agtcaccgag gcattcgtgc acggccacga catcgcggcg
    13801 gcggaaggcc ggcgttgcca gttcccgcgc cgccccctcg cgttcggcct gcgcgggctc
    13861 ctggacgtcc tccccctgta cgtggatcgg gagagggcgg cggacgcggt cgtctgtttc
    13921 gacgtgagga tccgcggcgg cgagcgagcc gggctggtgt tccatcgcgg caccctgacg
    13981 gtgcacgagg gcggcgcgcc ctcgcgcccc gactgcacgc tctgggtcga ccccggcggc
    14041 atgctgctgg tcggctacga gcgcgtctct ccgctcgccg ccgtcgcacg gggatgggtc
    14101 gcggcgtggg ggaggaagcc ctggctcggg ctcgcgcttc cgaagctcat tcgccagccg
    14161 taacgccggc tacgcggcgg ctccggggtc gagcgctccc gggccgagga gggccttcag
    14221 ctcggcatgg atgccgttcg acgtcgagac ccagaactcg ctgcccagct tgagggtcgt
    14281 cgtccggccg ctccgggcgc gcagatggag gaagacgggc gtgttgcccg gatgtgcccg
    14341 gagcacgtcc ttcagcttcg cgatgagcgt ttcactgcac gcatcggacg ggagcgtcac
    14401 ctcgagcggg ccctcgtcgc cgagcttcgg ctccttgacg tcttgagcga tcagcttgat
    14461 cgagtcgtcg cgcacgtcgc ggtcgagacg ccctcttatg aggaggatcg catcgggacg
    14521 aaggatcgct ccgaactgct ccacggtctt cgggaagcac agcacctcga cggtcgcgcc
    14581 ggacaggtcc tcgaccgtca ggatgttcat cacgccaccg ctcttcgtga cgcgcttctg
    14641 gtagccggcg acgagcccgc cgatcgtcac ggattctccc tgtgaccgcc cctcgagcgc
    14701 cgagatcggc ccatcggtca tgcggctcag gagtccctcg acgccgagga gcggatgatc
    14761 cgagacgtac agccccagca tctccttctc gtgcgccaac aacagctcct tcggatattc
    14821 gtcgcgtggg atcggcgtgt cctggtgcgc cccgccctcg ccgcatgccg cgccgaacaa
    14881 cgagaactgc cccgcctctt cctgcttgcg ccggtcgacg atgtcggcgc acagacgctc
    14941 gaacgtctcc gcgagccctt tccgcgtgtg gccgagcgac tcgaacgcgc ccgcgtggat
    15001 cagcgactcc accgtcttct tgttgagaca gatgtagtcg accttgcggc agaagtcgtg
    15061 gaacgactcg aaagccccct tgcgcaggcg agcgtcgatg atcttctcga cgacgccgtc
    15121 acccagcccc cgcacggcgg aaagcccgaa gcggatggag tcgcccgtgg gcgtgaagtc
    15181 cttctccgac gaattgacgt cggggatgag aacggggatc cccatcttcc gcgccatgtg
    15241 caggtacttc ggtttgtcgt ccttgcgatc cttgacgctc gtcagaagcg ccgccatgta
    15301 ctcgaccggg tagtgcgcct tcaaccacgc cgtctggtag gcgacgtagg cgtagcaggc
    15361 cgagtgcgcg cggttgaacg agtagtcggc gaacggctgg atcaacgccc acagcctctt
    15421 cgcgtccgcc tcggacatgc cggacgcggt gcagcctgag atgaagcgag gctcctcgcg
    15481 cgccatgatc tcgcggttct tcttaccgat cgccttgcgc accatgtcgg cctcgcccgg
    15541 cgaataaccg gcgaggatct gcaggagctg cactacctgc tcctgataca cgaggacccc
    15601 gtacgtctcg gcggtgatcc gttcgagcgc gggatgcagg tactcgacct tcgacgggtc
    15661 gtgcttgcgc tcgaggtact tggggatctc gtccatgggc ccgggacggt agagcgcgat
    15721 cagggccatg atctgctcga agcggtcggg cttgagctgc acgagcaacc gccgcatgcc
    15781 ctccgactcg agctggaaga ccccgtcgga gtcgccgcgt cgcaggatct cgtacacctt
    15841 cgggtcgtcg agcgggaggt cgtcgacgtc ggggcgcgtc ccgcggttcg cttcgacgta
    15901 gtcgagcgtc agctcgatca ccgagaggtt cctgagcccg aggaagtcca tcttcaggag
    15961 gcccagctct tcgacgccgt tcatgtcgaa ctgcgtgacg atctcgccgt gctcgccgcg
    16021 acgcaggggg acgtgctcga cgagagggtc acggccgatg accaccgccg ccgcgtggat
    16081 ccctgcggac cgcttgagat tctcgacctg cagggcggta tcgatgatct ccttcgccgc
    16141 gccgccgccc tgatacgccg cggccagctc gggggacgtc tcgagggcgc tccgcaggcc
    16201 tgagtcgcgc cccatgacaa gcggcgggta catcttggtg aggcggtcac cctccgcgta
    16261 ggggaagccc agcaccctcg ccgcgtcctt gatcgcctgc ttccccttga tcgtgccgta
    16321 ggtgacgatc tgtgcgacgc ggtcgtcgcc gtacttgtcc tgcacgtaac ggatcacgtc
    16381 gccgcgccgt cgatcgtcga agtcgatgtc gatgtcgggc atagcgacgc gtttggggtt
    16441 gaggaagcgc tcgaacatca ggtcgtactt gatgggatcg agttcggtga tcccgatcgc
    16501 gtaggagacg atcgagcctg ccgcgctgcc gcgcccggga ccgacgcgga tgccctgcct
    16561 cttcgcccag ttcacgaagt cctggacgac gaggaagtag ccggcgaagc ccatgtcggc
    16621 gatcacccgc agctcgtacc gcgcccgctc cgagacctcc gccggcagcg gctcgccgta
    16681 acggcggcgg catccgtcga gcacgagccg ctcgaggtac gagtcgagcg tctcgccggc
    16741 cgggacgtcg tagcgcggga gcagcaactt gtcgaaggtc atctcgacgt tgcaccgctc
    16801 tgcgatgtcg agcgtcgtct cgcacgcccc cggccaccgc gcgaaggcgc gctgcatctc
    16861 ctcgggagtc ttcagataga actcctccga gtcgaacttg aagcggttcg ggtcgctcag
    16921 ctcgctgccg gtgttgatgc acaagaggac ctcgtgcccg gcggcgtcgt ccttgtgggt
    16981 gtagtgggag tcgttggtgc agacccaggg gatgcccatc tcgcggccta tggcgacgag
    17041 ctcgtcgttc aacggaccct gctgcgcgag accgtgatcc tgtagctcca ggtagaaccg
    17101 gtcgccgaac aactcgcggt actgcgccac cttgcggcgc gccccggccg ggtcgccggc
    17161 gacgatcgca cgcgggatct cggcggagag gcatcccgac aggcagatga cgccctccgc
    17221 gtgctcggtc aggagctccc agtcggaacg gggttgatag tggaacccct cgagccacgc
    17281 ccgcgacgag atcttgacga ggttgccgta tcccgtctgg tccatggcca gcagcgtcag
    17341 gtggtaggtg cgctcgccgt tctcgcgctg cggcggcttc tcgcgccggt tcccgccgaa
    17401 gaggtagccc tccatcccaa ggatcggctt gacgccgtgg ctccggccgg accggtagaa
    17461 gtcgagcgcg ccgtacatca ctccgtggtc ggtgatcgca caggcgggca tgccggcgtt
    17521 cgcggcggct tcgaacatct cggagatgcg gctcgcgccg tcgagcatcg agtactcggt
    17581 gtgcgcgtgg aggtggacga agctcaaccc ggactgcctt tcgcgagccg ccgacgaatc
    17641 acaaacgtgt agttctatct actccgcccg ccgccgcggt gcaagcagcc gcgtcccacc
    17701 tgggaggacg tgtctgtcaa gcaccggttc ggcgcgccgg gcatgagcgt cgtcgctccc
    17761 ggctgtggat cacaaggggg cggaacctgt ggatttctcc tctacggcgg agagacgccg
    17821 gcgcgccggg cgacgtccgc caggtccgcc gggagggggt ccgtcacctc gacctcgccg
    17881 ccgtccgggt gcgggaaccg gagcaggtag gcgtggagga acggccgttc cagcccgagc
    17941 gagcgggcga ggtcgccctt gccgccgtag acggcgtcgc ccaggatcgg gaacccgaga
    18001 tgcgacaggt gcacccgtat ctgatgggtc cggccggtct cgagagcgac atcgagaagc
    18061 gccgtctcgt ctgtggcatc gacggcgcgg tagtgggtga cgctcggccg cccctcggcg
    18121 acgacggcca tgcgccggcg ccggaccgga tgtctgccca ccggcgcctc gatcgtgccg
    18181 ctcgctgcgg gcggtcgccc acgcacgagg gcgagatagc gcctctccac ttcccgctgc
    18241 cggatcgcct cgaccaggac ggccgcggcc ccgtcgtcct tcgctacgag caagagaccc
    18301 gaggtgtcct tgtcgaggcg gtggacgatg cccgggcgcg tcgatcccgc gcccgacagc
    18361 gggacgccca ggcccagcag cgcgttcacc agcgtcccgg cctcgtgccc ccgtgcggga
    18421 tgcgtgacga gacccggcgg cttgctgatc accaggacac gctcgtcctc gtagcggacg
    18481 ccgacgggga tgtcctcggc ctccggcggg cccgattcgg gcagggggat cgtcccggcg
    18541 acgacgtcgc cctcttcgag gcggtgcgaa ggcctgaccg ctaccccgcc gacggtaacg
    18601 agaccctcgc ggacggcccg ctgcaccagc gtgcgggtga cgccggcgcg ccccgccacc
    18661 acgacgtcca cgcggccccc cgcttcaccg ggcgcgacgg tgaaggagac cccggtcccc
    18721 gtcaccgctc cgccggcgcg gcccgccagc ccacgaccag cagaaccagc accccgaccg
    18781 tgatgcaggc gtcggccacg ttgaagatcg gccacacgtg gaggtcgacg aagtcgacca
    18841 cgcggccgcc cgtggcgcgg aagatccggt cggacacgtt gccgagccct cccccgagca
    18901 cgagg
//
LOCUS       DATLGG010000206         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_59959, whole genome shotgun sequence.
ACCESSION   DATLGG010000206 DATLGG010000000
VERSION     DATLGG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59959"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VM638_05915"
     CDS             complement(<1..749)
                     /locus_tag="VM638_05915"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HVM11993.1"
                     /translation="METSEAMPTGGAAAPDLADILRHEGPYLSLYLETAGDVETPRAR
                     SEQRWRSLRGSLEGADLPDDLLDTIGGIVADAHLQGPGLAVIATREEVRHVEHGSQPP
                     RSDVALVADLPALGPLLGWRLHAPAHMVVLADRTGADIFGFRYGRPDIHREAEGDDLH
                     VARSSPGGWSQRRFQERAENAWEENAEVAAAEVVRVAHAVDPRLIVVAGDVRAVNLLR
                     EALPPELATLIQEVGGGRAADGSREAIAEEAA"
     gene            816..1031
                     /locus_tag="VM638_05920"
     CDS             816..1031
                     /locus_tag="VM638_05920"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HVM11994.1"
                     /translation="MGVVARTPEPSITTYPAGPYLVRGPIRIRDESGCDLVVRRRVVA
                     LCRCGRSATKPLCDGSHAAPAAARRAD"
     gene            complement(1370..1987)
                     /locus_tag="VM638_05925"
                     /pseudo
     CDS             complement(1370..1987)
                     /locus_tag="VM638_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(1991..2983)
                     /locus_tag="VM638_05930"
     CDS             complement(1991..2983)
                     /locus_tag="VM638_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HVM11995.1"
                     /translation="MSLPRARGPLSGWVLERLDAAATPSPAPTVGDDPLAGDDLHLAL
                     YLCYELHYGGLPGVPADLEWDLDLLAFRGLLEARFEDAVRAALAPDVIPAGPIDRRLR
                     AVVAADDGPAVSRYLRRNGTVAEYREFLMHRSAYQLKEADPHAWAIPRLRGPAKVALV
                     EILGDEYGGGDPAWLHSQLFADSMRALELDATPLAYLDRLPGVTLAWVNLMSLFGLHR
                     RLRGALVGHLAAFEMTSCGPNRRYADGLRRLGYGEEATRYFDEHVEADAVHEAIACID
                     LAGSLVTAEPDLEDDVVFGARALLLTEAQLARHLLSCWSTGLSSLLDLRLPSSV"
     gene            3148..>3455
                     /locus_tag="VM638_05935"
     CDS             3148..>3455
                     /locus_tag="VM638_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HVM11996.1"
                     /translation="MIAALLTAASFAGLGGGDAAAQDPPPRTGFEQRNGSSWTTHAEE
                     LAFLSEVDRRSDRVELSVVGTTLQNRPIHLVRVGHPVPRDVAAAQTEPVVLFVCTQHG
                     "
BASE COUNT          502 a         1265 c         1262 g          426 t
ORIGIN      
        1 gccgcctcct ccgcgatcgc ctcgcgcgac ccgtcggccg cgcgtccgcc tccgacctcc
       61 tggatcagcg tcgccagctc cggcgggagc gcctcgcgca gcaggttcac cgcccgcacg
      121 tcgccggcga cgacgatcag ccggggatcc acggcgtggg cgacgcgcac gacctccgcg
      181 gccgcgacct ccgcgttctc ttcccacgcg ttctccgcgc gctcctggaa gcgccgctgg
      241 gaccagccgc ccggactcga ccgcgcgacg tgcagatcgt ccccctcggc ctcccgatgg
      301 atgtccggcc ggccgtagcg gaacccgaag atgtcggccc cggtccggtc ggcgagcacc
      361 accatgtgcg ccggagcatg gagccgccat ccgaggagcg gcccgagcgc cggaaggtcg
      421 gcgacgagcg ccacgtcgga gcgcggtggt tgggaaccgt gctcgacgtg acgcacctcc
      481 tcgcgggtcg cgatcacggc gaggccgggg ccctggaggt gcgcatcggc gacgatgccg
      541 ccgatggtat cgagcaggtc gtccggcaga tccgcccctt cgagcgagcc gcgcaacgac
      601 cgccaccgct gctccgaccg cgccctcggc gtctcgacgt ctcccgccgt ctcgaggtag
      661 agcgagaggt aaggtccttc gtgccgcagg atgtccgcga ggtccggcgc tgcggcgcca
      721 ccggtcggca tcgcctcaga ggtttccatg acgggttcct tcccccgggt tcccgccgct
      781 aacccgggag gtgtcgctca gggccgtgtg ggtagatggg cgtcgtggcg cgcacaccgg
      841 agccgagcat cacgacgtat cccgcggggc cctacctcgt gcgggggccg atccgcatcc
      901 gggacgagtc cgggtgcgac ctcgtcgtcc gccggcgggt cgtcgccctc tgccgttgcg
      961 ggcgctcggc gacgaagccg ctctgcgacg gctcccacgc ggcgcccgcc gctgcgcggc
     1021 gagccgactg acccaggcgc cgacgcagga cccgaagggg tgtcgccgcg cggcggagcc
     1081 gccgcccccg gtagcgcgcc cctccgtgga tcacccgagc cccgagagcg acccatgaac
     1141 agaccgcccg ctcgaggacc catgcgggtg cgaacagcgg ggcgcgcgcc gagaacgccc
     1201 gccggccgcc ggcacggcgc cggccgacct cggcgaggag cgtcagaccg gccgccgcgg
     1261 cgccgatccc gcgccacgcc cggcgatgca ccgccgcgcc gaccagcggg agcaccgaga
     1321 gcgagagcat gaggcgaacg ggctgcgcga ggtcgtcgta ggcctggcgc acgcgctggt
     1381 cgaggaacac ctccagcgtc ggagccaggc ggcggacgat gatctcggga gcgcgccgca
     1441 cgcgtccgcc cgcggccgtg atcgtccgca tcagctcgag gttctcgaac aggacgtcac
     1501 cgtcgtagcc cccgacgctg aggaacgagg accggcggat accgagcgtc cccgggtaat
     1561 cggtgtgcgt cgctcggttc aggagcgtgc gggccgtatc ccagagggca tgccaggtgt
     1621 cgggatccag gacgttctgc ggcacgacga ggtccgacgt ccgcagcagc tccgcgaccg
     1681 cacgcagctc cgcctcctcg tatcgcacgt cgtcgtccgc gatcacgacg agctcgtgca
     1741 tcgcctcccg cacgccggtg gtgacgccgt tcaccttgcc gttcgcgaac gcgaggtccg
     1801 gatgcggcgc gaggtgacgg atgcggtcgg ggaaggcggc cgcgtggtcg cggaacacgg
     1861 cttcgggcga accgtcgatg acgacgacct cggcgatgct cgcggttcgt gacaggtagg
     1921 cggccagctc ggcgtgctcg tgtccgctcc cgtcgcagcg gatcggcagg acgtaggaga
     1981 gctgcacgtc tcagacggag ctgggcagcc gcaggtcgag gagcgaggag agaccggtcg
     2041 accagcacga cagcaggtgg cgcgcgagct gggcctcggt gaggagcagg gcgcgcgctc
     2101 cgaacacgac gtcgtcctcg aggtcgggct ccgcggtgac gagcgagccc gcaaggtcga
     2161 tgcaggcgat cgcttcgtgg acggcgtcgg cctcgacatg ctcgtcgaag taccgcgtcg
     2221 cctcctcccc gtaccccaga cggcgcagcc cgtcggcgta gcggcggttc ggaccgcagg
     2281 aggtcatctc gaaggccgcg aggtgaccaa ccagggctcc gcggaggcgt cgatggagcc
     2341 cgaacaacga catcaggttg acccaggcga gcgtgacccc gggaaggcgg tcgaggtacg
     2401 cgaggggggt ggcgtcgagc tcgagcgccc gcatcgaatc cgcgaacagc tgcgagtgga
     2461 gccacgccgg atcgccgccg ccgtactcgt ccccgaggat ctcgaccagt gcgaccttcg
     2521 ccgggccgcg caaccgcggg atggcccacg cgtgcggatc ggcctccttc agctggtagg
     2581 ccgaacggtg catcaggaac tcccggtact ccgccaccgt gccgttccgc cggaggtacc
     2641 gcgacacggc cggcccgtcg tccgccgcca ccaccgcgcg cagccgtcga tcgatcgggc
     2701 ccgcgggaat cacgtccggg gccagcgcgg cgcggacggc gtcctcgaaa cgcgcctcga
     2761 ggaggccccg gaacgcgagc aggtcgaggt cccactccag gtcggcgggc acgccgggaa
     2821 ggccgccgta gtgcagctcg tagcagaggt acagcgccag gtggaggtcg tcgcccgcga
     2881 gcgggtcgtc tccgaccgtc ggggccggcg acggggtcgc ggcggcgtcg agccgctcga
     2941 ggacccagcc gctgagcggt ccgcgcgcgc gtgggaggct cacgccgcga cccttccccg
     3001 gccccaggcg tcgaaacgag cgaagcaggc gccttttggg caggtgcaca aaggggtcgg
     3061 gcggggcgat ggcgaaccac agggcaacac ggccgagcca ggggagccca gatgcagccc
     3121 acgcacacca cccgcctgag gagcgccatg atcgcggcgc tgttgaccgc cgcctcgttc
     3181 gccggcctgg gcggggggga tgctgcggcg caggacccac ctccacggac cgggttcgag
     3241 cagcggaacg ggtcctcgtg gacgacgcat gccgaggagc tcgccttcct ctccgaggtc
     3301 gatcgccggt ccgaccgggt cgagctctcg gtggtcggga cgaccctcca gaaccggccc
     3361 atccatctcg tccgcgtcgg ccacccggtc ccacgggacg tcgccgcggc gcagacggag
     3421 cccgtcgtgc tcttcgtctg cacccagcac ggcaa
//
LOCUS       DATLGG010000207         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_72316, whole genome shotgun sequence.
ACCESSION   DATLGG010000207 DATLGG010000000
VERSION     DATLGG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72316"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VM638_05940"
     CDS             <1..321
                     /locus_tag="VM638_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HVM11997.1"
                     /translation="GQRHPKTTLVYEYPRAEGDPYYPVPRPENAALYRRYKELAEATP
                     GVHFVGRLATYRYYNMDQVVAQALAVYARIAGPTRPTTTVNGNGGLKLDRDDDEPVAP
                     RRGR"
     gene            318..2510
                     /locus_tag="VM638_05945"
     CDS             318..2510
                     /locus_tag="VM638_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVM11998.1"
                     /translation="MTQEADARGLELWAGIECTRNRVGDRYLDQLELSGHDRRIEDLD
                     LFAELGVRSVRYPVLWEHVAPAGLEHADWRWSDERLERLRELGITPIVGLVHHGSGPP
                     HTNLLDPSFPEQLATFAGAVAERYPWVEMFTPVNEPLTTARFSGLYGHWYPHAKDHDS
                     FIRALAVQCRAVILAMRAIRERTPSARLIQTEDLGKTHSTPLLAYQAEMENERRWLSF
                     DLLCGRLDEASPVWPLVTSALEAEEIAWFRENPCPPDVLGINHYLSSERYLDEDLARY
                     PEAAHGGNHRHAYADVLAARVLADGPAGPRRLLEEAWERYGLPIAVTEAHNSGAREEQ
                     LRWLAEVWDGAAAAKAGGADIRAVTVWSVFGAFGWASLVTGGDLAYESGVYDLRAPAP
                     RPTLLVQACRALAAEGRFEHPVLASPGWWHRNDRYWYGRPPDLPDVGPMRGDARPLLV
                     VGGRGTLGRAFGRLCTARGLEARVVVREQMDLTDPASIAGALEEIRPWAVVNATGFVR
                     VDDAEVETEACHRANTLAPGLIAAACAERDLPLLTFSSDLVFDGESERPYVESDPVGP
                     LNVYGRTKAEGERLALGAHPRTLVVRTSAFFGPWDEYNFAVQAVRALRTGEGFVAAGD
                     ATVSPTYVPDLVWTSLDLLLDGETGIWHLANEGSMAWAGFARAAARTAGADPGGVREV
                     SMAELALPAPRPRWSVLGSERGWIMPSLEDALARWAVDMGWRSVPVGV"
     gene            2551..3093
                     /locus_tag="VM638_05950"
     CDS             2551..3093
                     /locus_tag="VM638_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM11999.1"
                     /translation="METNRYLGIYLNDHLAGATAGRELAKRCLSNNEGTPLGAYLETL
                     IDEIDEDRAALERLMERLDVPVSPWKRQALWLAEKVGRLKMNGQVFGYSDLSRVEELE
                     TLLLGVTGKLILWRSLAETGPQEPRLEVAELAVLAERAERQLRILEGHHRDAVKQAFQ
                     KPDIVERNRMQNVPIAEGDV"
BASE COUNT          470 a         1091 c         1180 g          433 t
ORIGIN      
        1 ggccagcgcc acccgaagac gacgctcgtc tacgagtacc cgcgggccga gggcgacccc
       61 tactatcccg tgccccgccc cgagaacgcg gcgctgtacc gccgctacaa ggagctggcg
      121 gaggcgacgc ccggcgtcca cttcgtcgga cggctcgcga cctatcgcta ctacaacatg
      181 gaccaggtcg tcgctcaggc gctggcggtc tatgcgagga tcgcggggcc cacgcgcccg
      241 acgacgaccg tgaacggcaa cggcggcctg aagctcgatc gcgacgacga cgagccggtc
      301 gccccgcgac gaggccggtg acccaagaag cggacgctcg cggcctcgag ctgtgggcag
      361 ggatcgagtg cacgcgaaac cgggtgggtg accgctacct cgaccagctc gagctgagcg
      421 gccacgaccg tcgcatcgaa gacctggacc tgttcgccga gctcggcgtc cgctcggttc
      481 gatacccggt cctgtgggag cacgtcgcgc ccgcgggtct cgaacatgcg gactggcgct
      541 ggagcgacga gcggctcgag cggctccgcg agctcggcat cacgccgatc gtgggcctcg
      601 ttcaccacgg cagcggcccg ccgcacacga acctgctgga tccgagcttt ccggagcagc
      661 tggcgacctt cgccggcgcg gtcgccgaac gctacccgtg ggtcgagatg ttcactccgg
      721 tgaacgagcc gctcaccacg gcgcgcttca gtgggctcta cggccactgg tacccgcacg
      781 cgaaggacca cgactcgttc atccgggcgc tggccgtgca gtgccgcgcc gtgatcctgg
      841 cgatgcgcgc gatccgcgag cggacgccga gcgctcggct gatccagacc gaggatcttg
      901 ggaagacgca cagcacgcct ctgctcgcct accaggccga gatggagaac gagcgccgat
      961 ggctgtcgtt cgatctgctg tgcggccgcc tcgacgaggc ctcgccggtc tggccgctcg
     1021 tgacgtcggc cctggaggcg gaggagatcg cgtggttccg cgagaaccca tgcccgccgg
     1081 atgtcctggg catcaaccac tacctcagca gcgagcggta cctggacgag gatctcgccc
     1141 ggtacccgga ggcggcgcac ggaggcaacc accggcatgc ctacgccgac gtcctcgccg
     1201 cgcgcgtgct ggcggacggg ccagctgggc cgcgacggct cctcgaggaa gcgtgggagc
     1261 ggtacgggtt gccgatcgcc gtgaccgagg cccacaactc cggcgcgcgg gaggagcagc
     1321 tgcggtggct ggcggaggtg tgggacgggg ccgccgcagc gaaggcgggc ggcgccgaca
     1381 tccgcgcggt gacggtgtgg tcggtgttcg gtgcgttcgg ctgggcgtcg ctcgtgacgg
     1441 ggggagacct cgcctacgaa tcgggtgtgt acgacctgcg ggcgcccgcg ccccgaccga
     1501 cgctcctcgt ccaggcatgc cgggccctgg cggccgaagg caggttcgaa cacccggtcc
     1561 tcgcctcgcc cggctggtgg caccgcaacg accgctactg gtacgggcgc ccgccggacc
     1621 ttcccgacgt cgggccgatg cgaggcgacg ctcgtccgct gctcgtcgtc ggcggccgcg
     1681 ggacgctggg gcgcgcgttc ggccgcctct gcacggctcg aggcctggag gcgcgggtcg
     1741 tcgtgcgcga gcagatggac ctgacggatc ccgcatcgat cgcgggggcg ctcgaggaga
     1801 tccggccctg ggccgtggtg aacgcgaccg gcttcgtccg cgtcgacgac gcggaggtcg
     1861 agaccgaggc ctgccaccgg gcgaacacgc tcgcgccggg cctgatcgcc gccgcctgcg
     1921 ccgagcgcga cctcccgctc ctgaccttct cgtccgacct cgtgttcgac ggcgagagcg
     1981 aacgcccgta cgtcgagagc gacccggtgg ggccgctcaa cgtgtacggc cggacgaagg
     2041 ccgagggcga gcgactcgcg ctcggggcac atccgcggac gctcgtggtc cgcacgagcg
     2101 ccttcttcgg gccctgggac gagtacaact tcgccgttca ggcggtccgc gcactgcgga
     2161 cgggtgaagg cttcgtcgcc gcgggggatg caacggtctc cccgacgtac gtgccggatc
     2221 tcgtatggac gtcgctcgat ctcctcctcg atggcgagac cgggatctgg catctcgcga
     2281 acgaggggtc gatggcctgg gccgggttcg cccgagcggc ggcccggacc gccggggcgg
     2341 atccgggcgg cgtccgggag gtgagcatgg cggagctggc gcttccggcg ccgcgcccgc
     2401 gatggagcgt cctcgggagc gaacgcggct ggatcatgcc atcgctcgag gacgccctcg
     2461 ctcggtgggc cgtcgacatg ggctggcgca gcgtgccggt cggcgtctga gagcgtgtcc
     2521 aaccgcggcc ccgggggtag gtacggaccc atggagacga accggtacct ggggatctat
     2581 ctgaacgacc atctggccgg cgcgacggcg ggccgcgagc tcgcgaagcg ctgcctctcg
     2641 aacaacgagg ggacgccgct cggcgcctac ctcgagacgt tgatcgacga gatcgacgag
     2701 gatcgcgcgg cgctcgagcg gctcatggag cgcctcgacg tgcccgtctc gccgtggaag
     2761 cggcaggcgc tctggctcgc cgagaaggtg ggccgcctca agatgaacgg tcaggtcttc
     2821 ggctactccg acctcagccg tgtcgaggag ctggagacgc tgctgctcgg cgtcaccggg
     2881 aagctgatcc tgtggcggtc gctcgcggag acgggcccgc aggagccacg cctggaggtc
     2941 gcggagctcg cggtcctcgc cgagcgtgcc gagcggcagt tgcggatcct cgaaggccac
     3001 caccgggacg ccgtgaagca ggcgttccag aagcccgaca tcgtcgagcg gaaccggatg
     3061 cagaacgtgc cgatcgcgga aggagacgtc tgatggccaa gcgtgcgcag cagccggagc
     3121 tcgcgcggag cgagcgtggc gacaccgaca tggacggcaa gcgggtcgct cggg
//
LOCUS       DATLGG010000208         4069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_72343, whole genome shotgun sequence.
ACCESSION   DATLGG010000208 DATLGG010000000
VERSION     DATLGG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72343"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VM638_05955"
     CDS             complement(<1..913)
                     /locus_tag="VM638_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVM12000.1"
                     /translation="MHPPDPATPEAGTLDAAGDRRTTWLLLGIILGAGLVLRLWDIDH
                     ALPFVYTADELRYFVPEAVRFVEEGYLPNRMGGNPPGLAYAFHAVYLAAFGGPEGILR
                     AWQASPETLFLIGRVVSALSGVVAAWLIFLTGTRLFGRRAGLVAAGLMVIAFLPFVYS
                     RRALADAAQLAPLALSLVGSAGILRRGRWTDYAIAGAGLGLATGTKYLSAIAALPLAA
                     AILPRFVDRRAWRPALLGAAIAVGAALVGLFLTHPFLLLRWGEVASQVPGLAGRGGGG
                     SPKVGIEPGGGVAYYLLVFTRGIGWVAT"
     gene            980..2041
                     /locus_tag="VM638_05960"
     CDS             980..2041
                     /locus_tag="VM638_05960"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HVM12001.1"
                     /translation="MKGLILAGGVGSRLRPLTHTQSKQLIPVANKPILYYGLEALAEA
                     GITEVGIVVGVLEEEIRGAVGDGSRWGLKVDYIHQESPLGLAHAVLTAQDYIGDEEFV
                     MYLGDNLVPEGLTDFVRAYEEHRPDAQIFLVKVPEPERFGVAVLEGDRVVRLVEKPKE
                     HISDLALAGVYIFNPTVFRAASAIEPSHRGELEITDAIQYLIDHGYRVRAEMITGWWK
                     DTGKMEDLIEANRIMLERREGSVRGSVDEESHLVGDVIVEEGATVRESVIRGPAIIGS
                     GAVVERSIIGPYVAVHDDTRIEDSEIENSILLSGSLISEVRKMSDSLVGKNVRIRKDD
                     ERPSVYRFMLGDNSEIRVP"
     gene            complement(2038..3198)
                     /locus_tag="VM638_05965"
     CDS             complement(2038..3198)
                     /locus_tag="VM638_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HVM12002.1"
                     /translation="MAHRQTGGGVPERTRRRLTVAAVVLGVVTLAGMIALRPGGGPRP
                     DLTELGVYRDVFAAEVVAAADQPCRGSAPEENIVCRRVTFELREGPDAGKRTSVDFPV
                     DAAVPPLAPGDGVVLGHTAGAEPGFEYSFVDRQRRPVLVWLALAFAAAVIALGRMRGL
                     AALAGLAASLLVLLAFILPAIVDGRSPVLVAVVGAAAIAYLALYLAHGFRPMTTVALL
                     GTLTSLALTALLAEAFVTMAELTGFASEEATIVRVGAAQIDLAGLTLAGVVIGALGAI
                     DDVTVTQASAVWEIRDADPKMPSRDLLRAGLRIGRDHVASTVNTLALAYAGASMPLLV
                     LFVLSQQPLGAIANGETVATEIVRTLVGSIGLVASVPITTWLAVRTATPRKR"
     gene            complement(3200..>4069)
                     /locus_tag="VM638_05970"
     CDS             complement(3200..>4069)
                     /locus_tag="VM638_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12003.1"
                     /translation="SGSPVAAVSLSLFDAGATTRLQTLTMLSGSRLGASFIVLLVGFL
                     YAMRSKDRRRSVGMGVLALTMTAIVYIPGMFAAYGLLRSGLLSGVGWQASEEVNGVID
                     AIWGPIVALADRVLPGPMLLPVGLAIILISFKLLDVVLPSLDGERHAGAREHWLKRPW
                     PMFFLGSLAALLTLSVSVALTILVPLASKGYVTRREAIPYIAGANITTLADTLVAAMI
                     LGNPVAVQVVLAEAIAVAVITVLILLTVYGPLQRTLIAFDDWIVATTPRLVAFVAALF
                     VLPVGLLFSGTFV"
BASE COUNT          670 a         1446 c         1485 g          468 t
ORIGIN      
        1 cggtggcgac ccacccgatt cccctggtga acacgagcag gtagtaggcg acccctccgc
       61 ccggctcgat ccccaccttc gggctgccgc cgccgccgcg tccggcgagc ccggggacct
      121 ggctggcgac ctcgccccag cggaggagga ggaacggatg ggtgaggaag aggccgacca
      181 gcgccgcccc cacggcgatc gcggcgccga ggagcgcggg gcgccacgcg cggcgatcga
      241 cgaaccgagg aaggatggcg gccgcgagcg gaagggcggc gatcgccgac aggtacttcg
      301 tcccggtggc gagccccagc cccgcaccgg cgatcgcgta gtcggtccag cggccgcggc
      361 gcaggatgcc ggcggagccg acgagcgaga gcgccagggg cgcgagctgc gcggcgtcgg
      421 cgagggcacg ccgcgagtac acgaacggca ggaaggcgat gaccatcagc cccgcggcga
      481 cgagccccgc gcggcgaccg aagagcctcg tgccggtgag gaagatgagc caggcggcga
      541 cgacgcccga cagggccgac acgacgcgcc cgatcaggaa cagcgtctcg ggcgaggcct
      601 gccacgcgcg gaggatgccc tcgggccccc cgaacgcggc gaggtagacg gcgtggaagg
      661 cgtacgcgag gccgggcggg ttcccgccca tgcggttggg caggtagccc tcctcgacga
      721 agcggacggc ctccgggacg aagtagcgca gctcgtcggc cgtgtagacg aacgggagcg
      781 cgtggtcgat gtcccacagc cgcaggacga gccccgcgcc gaggatgatc ccgaggagca
      841 gccaggtcgt cctgcggtcg cctgcggcgt cgagggtccc cgcctcgggc gtcgcgggat
      901 cgggcggatg catcggtgcc ggagtctagc cgcgggcccg gcggggcggc cggaggctgc
      961 ctgctatcct tggccggtca tgaagggact catcctggcc ggtggggttg ggagccggct
     1021 gcggccactc acgcacacgc agtcgaagca gctgatcccc gtcgcgaaca agccgatcct
     1081 ctactacggc ctcgaggcgc tcgccgaggc gggcatcacc gaggtcggga tcgtcgtcgg
     1141 cgtgctcgag gaggagatcc ggggcgcggt cggtgacggc tcgcgctggg ggctgaaggt
     1201 cgactacatc caccaggagt cgccgctcgg gctggcgcac gccgtcctca ccgcgcagga
     1261 ctacatcggc gacgaggagt tcgtcatgta cctcggcgac aacctcgtcc ccgaggggct
     1321 cacggacttc gtccgcgcgt acgaggagca tcgcccggac gcgcagatct tcctcgtcaa
     1381 ggtgccggag ccggagcgct tcggggtggc ggtcctcgag ggcgaccggg tcgtgcgcct
     1441 cgtcgagaag ccgaaggagc acatctccga cctcgcgctc gccggcgtct acatcttcaa
     1501 cccgaccgtc ttccgcgcgg cctcggcgat cgagccgtcg caccgcggcg agctcgagat
     1561 caccgacgcg atccagtacc tgatcgacca cggctaccgg gtccgcgccg agatgatcac
     1621 cggctggtgg aaggacaccg gcaagatgga ggacctgatc gaggccaacc ggatcatgct
     1681 cgagcgtcgc gagggaagcg tccggggttc ggtggatgag gagagtcacc tcgtcgggga
     1741 cgtgatcgtc gaggaaggag cgaccgtccg cgagagcgtg atccgcggcc ccgcgatcat
     1801 cggctcgggc gcggtcgtcg agcgcagcat catcgggccg tacgtcgcgg tccacgacga
     1861 cacccggatc gaggacagcg agatcgagaa ctcgatcctg ctctcgggct cgctgatctc
     1921 cgaggtgcgg aagatgagcg actccctcgt cggcaagaac gtgcggatcc gcaaggacga
     1981 cgagcgtccg tcggtctacc ggttcatgct cggcgacaac tccgagatcc gcgtccccta
     2041 gcgcttccgc ggcgtcgcgg tgcgcaccgc gagccaggtc gtgatcggca ccgacgcgac
     2101 gaggccgatg ctccccacga gcgtccgtac gatctcggtc gccaccgtct cgccgttcgc
     2161 gatcgccccg agcggctgct gcgagagcac gaagagcacg agcaggggca tcgacgcgcc
     2221 cgcgtaggcg agggcgagcg tgttcaccgt cgaggcgacg tggtcccggc cgatccgcag
     2281 gcccgcgcgc aaaaggtccc gcgacggcat cttcggatcg gcgtcccgga tctcccagac
     2341 cgccgaggcc tgggtcaccg tgacgtcgtc gatcgccccg agcgcgccga tcaccacccc
     2401 cgccagcgtc agtccggcga gatcgatctg cgccgccccg acccgcacga tcgtcgcctc
     2461 ctccgaggcg aacccggtca gctccgccat cgtgacgaac gcctccgcca gcagggccgt
     2521 gagcgcgagg ctcgtgagcg tcccgaggag ggcgacggtg gtcatggggc ggaacccgtg
     2581 cgccaggtag agggcgaggt aggcgatcgc cgcggcgccg acgaccgcca cgaggaccgg
     2641 gctccgcccg tcgacgatcg ccggcaggat gaacgcgagg agcacgagga ggctcgccgc
     2701 gagcccggcg agcgccgcga gaccgcgcat ccgaccgagc gcgatcaccg ccgcggcgaa
     2761 cgcgagcgcg agccagacca ggaccggccg tcgctgccgg tcgacgaacg aatactcgaa
     2821 ccccggctcc gctcccgccg tgtgcccgag gacgacgccg tccccgggcg ccaggggagg
     2881 caccgccgcg tcgaccggga agtcgacgct cgtccgcttg ccggcgtccg gtccctcccg
     2941 gagctcgaac gtgacgcgcc ggcacacgat gttctcctcc ggcgcgctgc cgcggcaggg
     3001 ctggtcggcc gccgcgacca cctcggccgc gaagacgtcg cggtacaccc cgagctcggt
     3061 gaggtccggg cgcgggccgc cgccggggcg cagcgcgatc atccccgcga gcgtgacgac
     3121 cccgagcacg accgccgcga ccgtcaggcg ccggcgcgtc cgctccggga cccccccgcc
     3181 ggtctgccgg tgcgccatct cagacgaagg tgccggagaa cagcaacccg accgggagga
     3241 cgaacagcgc ggcgacgaag gcgacgagcc gcggcgtcgt cgcgacgatc caatcgtcga
     3301 acgcgatgag ggtccgctgg agcggcccgt acaccgtcag gaggatcagc acggtgatca
     3361 cggcgacggc gatcgcctcg gcgagcacga cctgcacggc gaccgggttc ccgaggatca
     3421 tcgccgcgac gagcgtgtcg gcgagggtcg tgatgttggc gccggcgatg tacgggatcg
     3481 cctcccggcg cgtgacgtac cccttgctcg cgagcggcac gaggatcgtc agcgccaccg
     3541 agaccgacag cgtgagcagg gccgcgaggc tccccaggaa gaacatcggc cacggacgct
     3601 tcagccaatg ctcgcgcgcg cccgcatgtc gctcgccgtc gagggacggg agcacgacgt
     3661 cgaggagctt gaacgagatg aggatgatcg cgagcccgac gggcaggagc atcggacccg
     3721 ggaggacgcg gtcggccagg gcgacgatcg gcccccagat cgcgtcgatc acgccgttga
     3781 cctcctcgga cgcctgccat ccgacccccg acaggagccc cgagcgcagc aggccgtagg
     3841 cggcgaacat ccccgggatg tagacgatgg ccgtcatcgt cagcgcgagc acccccatgc
     3901 cgacgctccg ccggcggtcc ttgctgcgca tcgcgtagag gaacccgacg aggagcacga
     3961 tgaacgacgc gccgagccgc gagccgctca gcatcgtcag cgtctgcagg cgcgtcgtcg
     4021 cgccggcatc gaacagcgac agcgacaccg cggccaccgg cgaccccga
//
LOCUS       DATLGG010000209         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_6633, whole genome shotgun sequence.
ACCESSION   DATLGG010000209 DATLGG010000000
VERSION     DATLGG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6633"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VM638_05975"
     CDS             complement(<1..661)
                     /locus_tag="VM638_05975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVM12004.1"
                     /translation="MEAAFFDLDKTIISRSSSLALSRPMYRAGLVSRAQLLRGAYAQL
                     VYLLIGADERRMERAKNALLALTKGWERRRVEQVVRETLDNVLQPLIYQEALDLIALH
                     RALGRRVFIISSATEEIVRPLAERLGVEDVIATRVRIEGGRYTGELAFYCYGPAKAEA
                     IREVAAARGVDLAGSYAYSDSVTDLPMLEAVGNPIAVNPDRELRRVAIGRGWQTRDFR
                     RP"
     gene            849..1619
                     /locus_tag="VM638_05980"
     CDS             849..1619
                     /locus_tag="VM638_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12005.1"
                     /translation="MPRLVVAALLSLSLSSGTALAATPQIVDQAGDANGLNGQWYDPA
                     MLGKIAQEVRDIFLGGDPFGEGRPTPGSYAPADLRSVTLETEYTRTAVGADGWRHRPT
                     ALRIRFRTEDPAAAPHGATPLRLELHATLGPPFPDGCSSAFHIDVEADGTLSFARWFR
                     WEDCRWNTGASWGSFQHPAWTATRGADATEVIVRIPFASLTEEEHALLGPGAALRELQ
                     ATTQLWPPFTFDVTHRASGFVVGEDVPPDVPCTRDCGR"
     gene            1687..2613
                     /locus_tag="VM638_05985"
     CDS             1687..2613
                     /locus_tag="VM638_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM12006.1"
                     /translation="MAEQQRRDDGSRLDEVGRYLDMAARHPLLTKEDEAALAVVIERG
                     REARERLDSGRIRSEASRRELERAVREGEQARERFVLSNLRLVVSVAKKYQGQGLGLL
                     DLVQEGNIGLMRAVELFDHRKGFKFSTYATWWIRQAITRGIADRGRTIRVPVHVGDRL
                     RKLKATARRLLQETGEEPDVETLARAVEMPIDEVAKLLDLDPGDPVSLQAVVGEDTEL
                     GELIADDDEGPEATVVEGMTAEAIERAMNDLLGEQERRVLALRFGLDGAGARSLREIG
                     REIGISAERARQVEQKALTALRESDALRTDAA"
     gene            complement(2686..3060)
                     /locus_tag="VM638_05990"
     CDS             complement(2686..3060)
                     /locus_tag="VM638_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVM12007.1"
                     /translation="MAETRHYAARARSTDTPGRMICTARDHHWVIDGPAWNGFPHEAV
                     TPGESFLGAIAACCVEILQLVAAKEEVPLHDARVSVDAELDPDDQPHPQHTLFNRVRM
                     RVEVDGPTEEQAAALVDRFKGR"
     gene            complement(3087..>4134)
                     /locus_tag="VM638_05995"
     CDS             complement(3087..>4134)
                     /locus_tag="VM638_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM12008.1"
                     /translation="VVRRDVVFVTGTDEAAPFAGAVSDKNVLTHAFDAEDGSLLWTRI
                     YDGPSGSFEWGRMVETSPDGSRVFVGGIVTEGYWKYGVIAYDAATGDEVWTARHVFPD
                     SGRDGLLEDWPTAMAISPDGSTVGLTGRTIGSGGERHGTVLFDAADGSTRWSRVYEGD
                     GGPSEVPERIDIPPTFPVASVRLKGRAQQGNGLAFDPSGERVYVTGGGRVFPGGPVST
                     TIAYAAGSGEELWVARYGGLPGSIGASNAIAVSPSGDRIYVTGRTSQAGGRFIGSQHD
                     LPSSIDDVATVAYDRDGNEVWAARFDGANHLDMGEAISAHAGGVVVAGRSLVAEIPLL
                     PFANASLLVLSYDA"
BASE COUNT          605 a         1528 c         1442 g          559 t
ORIGIN      
        1 ccggccttcg gaagtccctc gtctgccacc ctcgtccgat cgcgacgcga cgcagctccc
       61 ggtcggggtt caccgcgatc gggttcccga cggcctcgag catcggcagg tcggtgaccg
      121 agtcggagta cgcgtacgag ccggcgagat cgacgcctct cgccgcggcg acctctcgga
      181 tcgcttccgc cttcgcgggc ccgtagcagt agaaggccag ctccccggtg tagcggccac
      241 cctcgatccg cacgcgggtg gcgatgacgt cctcgacccc gagccgttcg gcgagcggac
      301 gcacgatctc ctcggtggcg gatgagatga tgaagacccg ccggccgagc gcgcgatgca
      361 gcgcgatcag gtcgagcgcc tcctggtaga tcagcggctg gagcacgttg tcgagggtct
      421 cgcggaccac ctgctcgacg cggcgccgct cccagccctt cgtgagcgcg agcagcgcgt
      481 tcttcgctcg ctccatccgg cgctcgtcgg ccccgatcag gaggtagacg agctgcgcgt
      541 aggccccccg cagcagctgg gcgcggctga cgaggccggc gcggtacatc ggccgcgaca
      601 gggcgagcga gctggacctc gagatgatcg tcttgtcgag gtcgaagaac gccgcctcca
      661 tggctccatc gtaccgtcgc caccccatcg gcccgtggtg cctgctccgc tgctcgcgag
      721 ctggcggccg gaccgcctcc tccggcggga acccgcggca ggagacggcg gccggtgcgc
      781 agaagagggg tgccgacggc ccgccgccgg acggatgcgc ccacgggtcg tcgcgggaag
      841 ggaggaccat gccgcgtctc gtcgtcgccg cgttgctgtc gctctcgctt tcgtccggga
      901 cggccctcgc cgccacgccg cagatcgtcg accaggcggg tgacgcgaac ggcctgaacg
      961 ggcagtggta cgacccggcg atgctcggga agatcgccca ggaggtccgc gacatcttcc
     1021 tgggtggcga ccccttcggc gagggacgcc cgacccccgg gtcgtacgcg ccggccgacc
     1081 tgcgctcggt gacgctcgag acggagtaca ccaggacagc ggtcggagcc gacgggtggc
     1141 gacaccgacc gacagcgctg cgcatccggt tccggaccga ggaccccgcc gccgcgccgc
     1201 acggggcgac cccgctgcgc ctcgagctgc acgccaccct cggcccgccg ttccctgacg
     1261 gctgctcctc cgcgttccac atcgacgtgg aggccgatgg aaccctctcc ttcgcgcggt
     1321 ggttccgctg ggaggactgc cgctggaaca ccggcgcgag ctggggcagc ttccagcatc
     1381 ccgcctggac cgccacccgc ggcgccgacg cgaccgaggt catcgtccgg atccccttcg
     1441 cgtccctgac cgaggaggaa cacgccctgc tggggcccgg agcagcgctg cgggagctcc
     1501 aggcgacgac ccagctctgg ccgcctttca ccttcgacgt gacccaccgc gcgagcgggt
     1561 tcgtcgtggg cgaggacgtc ccacccgacg tcccctgcac ccgcgactgc ggccgctgac
     1621 cggctgcggg tctccgaccg cttgggtggg aataacccgc ccgtgcaggg cgttgtcacc
     1681 gttcccatgg cagagcagca gcgacgcgac gacgggtcgc gcctcgacga ggtcggacgc
     1741 tacctcgaca tggccgcgcg ccacccgctc ctgacgaagg aggacgaggc ggcgctcgcg
     1801 gtcgtgatcg agcgtgggcg ggaagcacgc gagcgcctgg attccggccg catccggtcg
     1861 gaggcgagcc ggcgtgagct cgagagggcg gtccgcgagg gcgagcaggc ccgcgagcgg
     1921 ttcgtcctgt ccaacctccg cctcgtcgtg tcggtcgcga agaagtacca ggggcagggc
     1981 ctcggtctcc tcgacctcgt ccaggagggg aacatcggcc tgatgcgcgc ggtcgagctg
     2041 ttcgaccacc gcaagggctt caagttctcg acctacgcca cctggtggat ccgccaggcg
     2101 atcacccgcg gcatcgccga tcgcgggcgg acgatccgcg tgccggtgca cgtgggcgac
     2161 cgcctgcgga agctcaaggc cacggcgcgg cggctgctcc aggagacggg cgaggaaccc
     2221 gacgtcgaaa cgctcgcccg ggcggtcgag atgccgatcg acgaggtcgc gaagctgctc
     2281 gacctcgacc ccggggaccc ggtctccctt caggcggtcg tgggcgagga caccgagctc
     2341 ggcgagctga tcgccgacga cgacgaaggg cccgaagcga ccgtcgtcga gggcatgacc
     2401 gccgaggcca tcgagcgggc gatgaacgac ctgctcggcg agcaagagcg acgcgtgctg
     2461 gcgctgcggt tcggcctcga cggcgcgggc gcgcgctccc tccgcgagat cggccgggag
     2521 atcggcatct ccgccgagcg cgctcggcag gtcgagcaga aagccctcac cgcgctgcgg
     2581 gagtccgacg cccttcgcac ggacgccgcc taggcgtcag cgcggccgag cctcgatctc
     2641 gatcctgacg tccggggtcg cgaccgcgac cgaaccgtag agcggtcacc tgcccttgaa
     2701 gcggtcgacg agcgccgctg cctgctcctc cgtcggtccg tcgacctcca cccgcatccg
     2761 gacccggttg aagagcgtgt gctgcgggtg cggctgatcg tcggggtcga gctcggcgtc
     2821 gacgctcacg cgcgcgtcgt gcagcgggac ctcctccttc gccgcgacga gctgaaggat
     2881 ctcgacgcag cacgcggcga tcgcaccgag gaagctctcg cccggagtca ccgcctcgtg
     2941 cgggaacccg ttccacgccg gcccgtcgat cacccaatgg tggtcccgcg ccgtgcagat
     3001 catccgcccg ggggtatcgg tcgagcgcgc acgcgctgcg taatgccggg tctccgccat
     3061 ccggcggaac gtaccaccgg acggcctcag gcgtcgtagg agaggacgag gaggctggcg
     3121 ttcgcgaacg gcaggagtgg gatctccgcg acgagcgagc gacccgcgac gacgacgccg
     3181 ccggcgtgag ccgagatagc ctcgcccata tcgaggtggt tcgcgccgtc gaaccgcgcc
     3241 gcccagacct cgttcccatc gcggtcgtac gcgaccgtcg ccacgtcgtc gatggagctg
     3301 gggaggtcgt gctgggagcc gatgaaacgc ccaccggcct gggacgtccg acccgtcacg
     3361 tagatccggt cgccggaggg cgagaccgcg atcgcgttcg acgcgccgat gctcccgggc
     3421 aggcccccgt accgcgcgac ccagagttcc tcccccgatc ccgccgcgta ggcgatcgtc
     3481 gtggagaccg gaccacccgg gaagacacgg ccgccaccgg tgacgtacac gcgctccccc
     3541 gacggatcga acgccagccc gttcccctgc tgggcccgcc ccttcagtcg gaccgaggcg
     3601 acggggaagg tgggcgggat gtcgatccgc tccggaacct cgctcggccc gccgtcgccc
     3661 tcgtacaccc ggctccaacg cgtcgagccg tcggcggcgt cgaacaggac ggtgccgtgt
     3721 cgctcaccgc cggagccgat cgtcctcccg gtgagcccga ccgtcgagcc atccggcgag
     3781 atcgccatcg cggtcggcca gtcctccagg aggccgtcgc ggccggagtc cgggaagacg
     3841 tgtcgggccg tccagacctc gtcgccggtg gcggcatcgt acgcgatgac cccgtacttc
     3901 cagtacccct ccgtcacgat gccgcccacg aacacgcggg acccgtcggg ggaggtctcg
     3961 accatcctcc cccactcgaa gctcccactc ggcccatcgt agatgcgggt ccacaggagc
     4021 gacccatcct cggcgtcgaa cgcatgggtc aggacgttct tgtcgctgac agcccccgcg
     4081 aacggcgccg cctcgtccgt cccggtcacg aacacgacgt cgcgacgcac gacc
//
LOCUS       DATLGG010000210         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_170557, whole genome shotgun sequence.
ACCESSION   DATLGG010000210 DATLGG010000000
VERSION     DATLGG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170557"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VM638_06000"
     CDS             complement(<1..353)
                     /locus_tag="VM638_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVM12009.1"
                     /translation="MRRNLFAKVAVLAAIALVTGACQRGGDGGNAGGEGGGEGQGGRR
                     LLQTVQERGTLRCGVNETVPGFGFLNPDSGEIEGFDIEFCKAIAAAVLGDAEAHELVP
                     VSAEERLSALRAGNY"
     gene            complement(485..2020)
                     /gene="purH"
                     /locus_tag="VM638_06005"
     CDS             complement(485..2020)
                     /gene="purH"
                     /locus_tag="VM638_06005"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVM12010.1"
                     /translation="MRSIPVARALVSVSDKEGLADLAAGLADAGVAIVSTGSTAEAIR
                     GGGVPVTNVAEVTGFPELMEGRVKTLHPAIHAGILADKEKPEHLRAIEEHGIEPFDLV
                     VVNLYPFAKTVASGAAREEVIEQIDIGGPTLVRAAAKNHGSVGIVVSPDRYGEVLEAI
                     RTGGLPPELRASLAADAFAHVAAYDAAISAWFDTWSPRERFPESTTLGLERLQELRYG
                     ENPHQPAALYRTLPGPGPLGGADVLQGKEMSFNNWLDAEAARALAATFDEPAAVIVKH
                     NNPCGAAVADSLAEAYRRAFASDEVSAFGGVVAFNGEVDAAAAAAMAEVFTEVVVAPA
                     YVGEALEAFAARTNLRVVVAPAPEALGIEVRSIDGGALVQSRDTVTEERAEMQVVTKR
                     EPTEEEWADLLFAWRVAARVKSNAIVLVSDRATVGVGAGQMSRVDAVDIAARKAGERA
                     RGAVLASDAFFPFRDGIDRAAEAGVRAAIHPGGSVRDEEVTAAADEHGIAMVLSGRRH
                     FRH"
     gene            complement(2020..2625)
                     /gene="purN"
                     /locus_tag="VM638_06010"
     CDS             complement(2020..2625)
                     /gene="purN"
                     /locus_tag="VM638_06010"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HVM12011.1"
                     /translation="MGARIAVLASGSGTNLQALLDEPAVGERVAVVVSDRGEAGALQR
                     AEERGIPALHLDPGAHASREDYDAALLDVLRERADLVCLAGFMRILSAPVVRAFERRM
                     LNVHPSLLPAFPGAHAVRDALAWGVKVTGVTVHLVDEEVDHGPIVLQEAVPVAAEDDE
                     RSLHARIQQVEHRLFPRAAALLADDRLRVEGRTVVVVGEER"
BASE COUNT          383 a         1068 c          941 g          353 t
ORIGIN      
        1 tcgtagttgc cggcgcgcag cgcgctcagc cgctcctcgg cgctcaccgg aacgagctcg
       61 tgggcctccg cgtcgccgag gacggcggcc gcgatcgcct tgcagaactc gatgtcgaac
      121 ccttcgatct caccgctgtc cgggttgagg aagccgaacc cgggcacggt ctcgttcacg
      181 ccgcagcgca gcgtgccgcg ctcctgaacc gtctggagca ggcggcggcc accctggccc
      241 tcgccccctc cttctcctcc cgcgttcccg ccgtccccac cacgctggca ggcgccggtc
      301 acgagggcga tcgcggcaag caccgcgacc ttggcgaaga gattcctccg catgatcccc
      361 tcccttcgac tcgctggccc aggacgttcg ggcgctcccg ggccggtgct cccggagcat
      421 agtcgattcg ccgggatgcc taccacctgg gaggaagggc ccgatacggc cgggatcggc
      481 cggttcagtg ccggaagtgc cggcgcccgg agaggaccat cgcgatgccg tgctcgtcgg
      541 cggcggcggt gacctcctcg tcccggacgg acccccccgg gtggatcgcg gcacggaccc
      601 cggcctcagc ggcgcggtcg atcccatcgc ggaaggggaa gaaggcgtcc gaggcgagga
      661 cggcgccacg ggcccgctcc cccgccttcc gggcggcgat gtccaccgcg tccacccggc
      721 tcatctgccc cgcgccgacg ccgacggtcg cgcggtccga cacgaggacg atcgcgttcg
      781 acttcaccct cgccgcgacg cgccaggcga acagcaggtc cgcccactcc tcctcggtcg
      841 gctcccgctt ggtcacgacc tgcatctccg cccgctcctc cgtcacggtg tcgcgcgact
      901 gaaccagagc gcctccgtcg atcgagcgga cctcgatccc gagcgcctcg ggggccggcg
      961 cgacgaccac gcgcaggttc gtgcgcgccg cgaaggcctc gagggcctcc cccacgtacg
     1021 cgggcgccac gacgacctcg gtgaacacct cggccatcgc cgcggcagcg gcggcgtcga
     1081 cctcgccgtt gaaggcgacg acgccaccga acgcggaaac ctcgtccgag gcgaacgccc
     1141 gccggtacgc ctccgccagc gaatcggcca cggcggcccc gcaggggttg ttgtgcttca
     1201 cgatcaccgc cgccggctcg tcgaaggtcg cggcgagggc gcgcgccgcc tcggcgtcga
     1261 gccagttgtt gaacgacatc tccttgccct ggaggacgtc ggcgcccccg agcggtcccg
     1321 ggcccggcag ggtccggtac agggccgccg gctggtgcgg gttctcgccg taccgcagct
     1381 cctggagccg ctcgaggccc agggtcgtgc tctcggggaa ccgctcgcgc ggggaccagg
     1441 tgtcgaacca ggccgagatc gccgcgtcgt aggcggccac gtgggcgaag gcatccgcgg
     1501 cgaggctcgc gcggagctcc ggcggcagcc cgcccgtccg gatcgcttcg agcacctccc
     1561 cgtaccggtc gggcgagacc acgatcccga ccgagccgtg gttcttcgca gcggcccgca
     1621 cgagggtcgg tccgccgatg tcgatctgct cgatcacctc ctcccgcgcc gctccggagg
     1681 ccaccgtctt cgcgaagggg tacaggttga cgacgacgag gtcgaacggc tcgatcccgt
     1741 gctcctcgat cgcgcgcagg tgctccggct tctccttgtc ggcgaggatc cccgcgtgga
     1801 tcgcggggtg gagggtcttc acgcggccct ccatcagctc cgggaacccg gtgacctccg
     1861 cgacgttcgt caccggaacc ccccctccgc ggatcgcttc ggccgtggaa ccggtcgaga
     1921 cgatcgcgac gccggcatcg gcgaggccgg cggcgaggtc cgccagcccc tccttgtccg
     1981 agaccgagac gagcgcgcgc gcgaccggga tcgagcgcat cagcgctcct ccccgacgac
     2041 cacgaccgtc cgtccctcca cgcgcagccg atcgtcggcc aggagcgcgg cggcccgtgg
     2101 gaacagccgg tgctcgacct gctggatgcg ggcgtgcagc gaccgctcgt cgtcctcggc
     2161 ggcgacgggg accgcctcct gaaggacgat cgggccgtga tcgacctcct cgtccaccag
     2221 gtggacggtg acgcccgtga ccttcacccc ccaggcgagc gcgtcgcgga cggcgtgcgc
     2281 ccctgggaac gccgggagca gcgacgggtg cacgttcagc atccggcgct cgaaggcccg
     2341 caccaccgga gccgacagga tccgcatgaa ccccgccagg cacacgaggt ccgcacgctc
     2401 gcggaggacg tccagcagag cggcgtcgta gtcctcccgg gaggcgtgcg cccccgggtc
     2461 gaggtggagg gccgggatcc cgcgctcctc cgcccgctgc agggctccgg cctcgccgcg
     2521 atcggagacg acgacggcca cacgctcccc gacggccggc tcgtcgagga gcgcctggag
     2581 gttcgtcccc gaccccgaag caaggaccgc gatccgcgca cccacgcggc gaacctacgg
     2641 aggcagtccc acggctgtca acgcggcagc gggagccccg cgggggctac tatccccgag
     2701 ccccccggga gcgacgatgg agaactcgac cacagcaccc accaa
//
LOCUS       DATLGG010000211         3120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_72697, whole genome shotgun sequence.
ACCESSION   DATLGG010000211 DATLGG010000000
VERSION     DATLGG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72697"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VM638_06015"
     CDS             <1..452
                     /locus_tag="VM638_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12012.1"
                     /translation="SAGVVKASTLFWGRNAPLVTLLKRRGDREIGRGCWAAKISTDPV
                     FGRLYVGEIFVAHLAPTAPHAFRVDVARGPADPFEVLGRLAGCADDAAFVGYPYPLAR
                     AHQAARVSGYAVADLRRGFRDALSRAGMSEDDIEVLFQDFHEVLNRS"
     gene            454..2019
                     /locus_tag="VM638_06020"
     CDS             454..2019
                     /locus_tag="VM638_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12013.1"
                     /translation="MDYKGRIFGKSVLEARFRAFHGTDLFLGELLAAEDPERARRYLF
                     RVIDVVYGSDSSDPAWAERTAGAYMAGDAAGSPYTLRDPEQRLHKVAMCAPLGYIDAA
                     GSFRKPKSLPSQFTRVTAPTAEDFDFLRERMGDLRVGLLRSGEDTIDLEVGVRGETLT
                     SHVGVFATTGMGKSNLMKVLAGTILESRGRYACLLFDPHGEYLEGGGGTARGLRHHPW
                     AVDRLKVYATRAAGAAATALRLSLAELTLDDLHTAYDWSRAQEEAMYRLERLYREDWI
                     PTIADEGQDLQDLAEQTGSSTSTLQVVQRRARRILDLPCISRDLSQVSLSERIVEELS
                     NGYSVLVDSSGLSDMEEILVASVITRRVLGAYGNAYLEDREAFDRLPPTLIALEEAQR
                     VLSKVKRSDANVFPRVAREGRKFKVGLCAVSQQPKLIDDELLSQFNTFFVLGLADEKD
                     RTILRGSSKQDIKDLGPEIQTLMPGEAIVTNLEAPFALPARVHLYEDYLKAVKGPAPL
                     PEKPKDAGRGFVE"
     gene            2016..>3120
                     /locus_tag="VM638_06025"
     CDS             2016..>3120
                     /locus_tag="VM638_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVM12014.1"
                     /translation="MRNVGRLEGIPGFNIDKVAAAAGDDPEVLRMENLDTDLDPPAGV
                     REATKEAVDRSEANSWLPFDGKPEMKEAVAGYIERRSGQRYDPLGEVVITSGEGEAWL
                     NALLVVTDPGDEVVVTDPTYAGMINRVRLAGAVPKPVPLLPSQDGWRLDLDALGAAVG
                     PRTRAVFVMNPSLPTGCVLTHDEWKAIADLCRRHDLWLIYAQLWEGIVFDGLPIVHPA
                     SLPDIRDRLIVAGSTVCEHRTIGWRIGWTVAPEGLRSDLSKTQIYNGLVPSGILQAGV
                     RVALGEPDEDLARAVAEYERRRDETLRQLEGFPVVPASGGWMVLMETASMGIDPAEAS
                     RRMLEQKVAVTQMRGWGADVAERYLRFVYSREPV"
BASE COUNT          462 a         1086 c         1123 g          449 t
ORIGIN      
        1 acagcgcagg ggtcgtgaag gcgtccacgc tgttctgggg acgcaacgcc cccctcgtga
       61 ccctgctgaa gcggcgtggg gaccgcgaga tcggccgggg gtgctgggcc gcgaagatct
      121 cgacggaccc cgtcttcgga cgcctctacg tcggggagat cttcgtggct cacctcgcgc
      181 ccaccgcgcc gcacgccttc cgcgtcgacg tggctcgggg gccggcggat ccgttcgagg
      241 tcctcggccg cctcgcgggc tgcgccgacg acgcggcctt cgtcggctac ccctatcccc
      301 tggcgcgagc gcatcaggcg gcgcgcgtca gcggttacgc cgtcgccgac ctgcggcgag
      361 ggttccgcga cgccctctcg cgcgccggca tgtccgagga cgacatcgag gtcctgttcc
      421 aggacttcca cgaggtgctg aaccggagct gacatggact acaagggcag gatcttcgga
      481 aagagcgtgc tcgaagcgcg cttccgcgcg ttccacggaa cagacctgtt cctcggcgag
      541 ctgctggcgg ccgaggaccc cgagcgcgcc cggcgctacc tcttccgcgt gatcgacgtc
      601 gtctacggca gcgactcctc cgatccggcc tgggccgagc ggacggccgg cgcgtacatg
      661 gcgggcgacg ccgcggggag cccctacacc cttcgggacc ccgagcagcg cctccacaag
      721 gtcgcgatgt gtgcgccgct cggctacatc gacgccgcgg gatccttccg caagccgaag
      781 tccctcccct cgcagttcac ccgcgtgacg gcgccgacgg ccgaggactt cgacttcctg
      841 cgcgagcgga tgggcgacct gcgcgtcggc ctcctccgct ccggggagga cacgatcgac
      901 ctcgaggtcg gggtccgcgg cgagaccctc acgtcgcacg tcggcgtctt cgcgaccacg
      961 ggtatgggca agtcgaacct gatgaaggtc ctcgccggga cgatcctcga gtcgcgtggg
     1021 cgctacgcgt gcctgctgtt cgacccgcac ggtgagtacc tcgagggtgg cggagggacg
     1081 gcccgcggcc tgcggcacca cccctgggcc gtcgaccgcc tcaaggtcta cgcgacgcgc
     1141 gccgccgggg ccgcggcgac agcgctccgg ctgtcgctcg cggagctcac gctcgacgac
     1201 ctccacacgg cgtacgactg gagccgcgcg caggaggagg cgatgtaccg cctcgagcgt
     1261 ctgtaccgcg aggactggat cccgacgatc gccgacgagg ggcaggacct ccaggacctc
     1321 gcagagcaga ccggctcgtc gacctccacg ctccaggtgg tccagcgccg ggcgcggcgg
     1381 atcctcgacc tcccctgcat cagccgcgac ctctcccagg tgtcgctgtc ggagcgcatc
     1441 gtggaggagc tctcgaacgg ctactcggtg ctcgtggact cgagcgggct ctccgacatg
     1501 gaggagatcc tcgtcgcgtc cgtgatcacg cggcgggtcc tcggcgccta cggcaacgcc
     1561 tacctcgagg accgggaagc gttcgaccgg ctgccgccca ccctgatcgc cctcgaggag
     1621 gcccagcggg tcctctcgaa ggtcaagcgc tccgacgcga acgtgttccc gcgcgtcgcg
     1681 cgcgagggcc ggaagttcaa ggtcggcctc tgcgcggtgt cgcagcagcc gaagctgatc
     1741 gacgacgagc tgctctcgca gttcaacacg ttcttcgtcc tggggctcgc cgacgagaag
     1801 gaccggacga tcctgcgcgg ctcgtcgaag caggacatca aggacctcgg gccggagatc
     1861 cagacgctca tgcccggcga ggcgatcgtc acgaacctcg aggctccgtt cgctctcccg
     1921 gctcgggtgc acctgtacga ggactacctc aaggcggtga agggcccggc gcccctgccg
     1981 gagaagccga aggacgcggg aagggggttc gtggaatgag gaacgtcgga cggctcgaag
     2041 gcatccccgg gttcaacatc gacaaggtgg cggccgccgc gggtgacgac cccgaggtcc
     2101 tgcggatgga gaacctggac acggatctcg atccccccgc gggcgtccgc gaggcgacga
     2161 aggaggcggt cgaccggtcc gaggcgaaca gctggctgcc gttcgacgga aagccggaga
     2221 tgaaggaggc cgtcgccggc tacatcgagc gtcggagcgg ccagcggtac gacccgctgg
     2281 gcgaggtcgt catcacgagc ggcgagggtg aggcgtggct gaacgcgctc ctcgtcgtca
     2341 ccgatcccgg ggacgaggtg gtcgtcaccg accccaccta cgccgggatg atcaaccgcg
     2401 tccgcctggc gggcgccgtg ccgaagccgg tgccgctcct cccgagccag gacgggtggc
     2461 ggctcgacct cgatgcgctc ggtgccgcgg tgggtccgag gacgcgcgcg gtcttcgtga
     2521 tgaacccgtc gctgccgacc gggtgcgtgc tgacgcacga cgagtggaag gccatcgcgg
     2581 acctgtgccg ccggcacgac ctctggctga tctacgcgca gctgtgggag ggcatcgtct
     2641 tcgacgggct cccgatcgtc cacccggcgt cgttgcccga catccgtgac cgcctgatcg
     2701 tcgccggctc cacggtgtgc gagcaccgca cgatcggctg gcggatcggc tggaccgtcg
     2761 cgccggaggg cctccgctcg gacctgtcga agacgcagat ctacaacggg ctggttccca
     2821 gcgggatcct ccaggcgggc gtcagggtcg cgctcggcga gccggatgag gatctggcac
     2881 gcgccgtcgc ggagtacgag cggcggcgag acgagacgct gcggcagctc gaggggttcc
     2941 cggtggtgcc cgcatccggc ggatggatgg tcctcatgga gaccgcctcg atggggatcg
     3001 acccggccga ggcctcgcgc cggatgctcg agcagaaggt ggccgtcacg cagatgcggg
     3061 gctggggcgc cgacgtggcg gagcggtacc tgcggttcgt ctacagccgc gagccggtcg
//
LOCUS       DATLGG010000212         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_196040, whole genome shotgun sequence.
ACCESSION   DATLGG010000212 DATLGG010000000
VERSION     DATLGG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196040"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VM638_06030"
     CDS             <1..581
                     /locus_tag="VM638_06030"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVM12015.1"
                     /translation="GAQSLVRDFLSGFFILLENQFGIGDVIDVTATGATVTGKVEQIS
                     LLTTSLRSYDGTLHIVPNGSIQLVGNRYKGWARAIVDIGVAYGEDLERVKGVLEGLFE
                     ELRADRELAPGFLSGPDVLGVERMTDYEIVLRIVAEVRPSRRFDIERQLRRRIKERFD
                     REGVEIPFPHQVMISRQEEPAKGQPGRPDRAG"
     gene            724..879
                     /locus_tag="VM638_06035"
     CDS             724..879
                     /locus_tag="VM638_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12016.1"
                     /translation="MNGVAKQFWSEVMDIRSPLPDDGRGRKVARKGRKIQRRTVSTAR
                     RTGRPAA"
     gene            911..1102
                     /gene="rpmB"
                     /locus_tag="VM638_06040"
     CDS             911..1102
                     /gene="rpmB"
                     /locus_tag="VM638_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HVM12017.1"
                     /translation="MAAVCEICGRKPGFGMQVSHSHRRSNRRWSPNVQKIRVLINGAP
                     KRAHVCTKCIKAGKVTRAI"
     gene            complement(1115..>2186)
                     /locus_tag="VM638_06045"
     CDS             complement(1115..>2186)
                     /locus_tag="VM638_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12018.1"
                     /translation="ISGNGRYVAFHSMASNLVPGDTNETMDTFVHDRVTGGVDRVSVS
                     ESGEEGNHWSASGRPAFARGGREVIFATDAWNLLPETQEGDREVVARDRGPDLGVGAI
                     SLGSVDGQVRVGGWASFSGAVLASAADPADDGAGAAPRVGGEITAASAVLRPEAEDLL
                     LRIDVSAIPGARPAAAPAAGGSATGWAGLPAVRYGFEIAAGPVRFEARVLRSTENDVP
                     LGPTFHLYRCEPDCVRVGGLRGSYGNTGDSVRVALPLDRLGLEPGTPLSLRAHTALAE
                     SGGDPFLPLDEVTLPAATLAAPSVAVGTAPQGAPYEEVGFSPASLDSGRFTATLAEPE
                     PNHDLWVRACLAETCSWTRREL"
BASE COUNT          348 a          785 c          778 g          275 t
ORIGIN      
        1 tcggggcgca gagcctcgtc cgggacttcc tgtcggggtt cttcatcctg ctcgagaacc
       61 agttcggcat cggcgacgtg atcgacgtga ccgcgacggg agcgaccgtc accgggaagg
      121 tcgagcagat cagcctgctc acgacgtcgc tccgctccta cgacgggacg ctgcacatcg
      181 tgccgaacgg cagcatccag ctggtcggca accgctacaa gggctgggcg cgggcgatcg
      241 tcgacatcgg cgtcgcctac ggcgaggacc tcgagcgggt gaagggggtc ctcgaggggc
      301 tcttcgagga gctccgggcc gaccgcgagc tcgcgcccgg cttcctctcc ggccccgatg
      361 tgctcggggt cgagcggatg accgactacg agatcgtgct ccggatcgtc gcggaggtcc
      421 gcccgtcccg gcggttcgac atcgagcgcc agctccgccg tcgcatcaag gagcggttcg
      481 accgggaagg cgtcgagatc cccttcccgc accaggtcat gatctcgcgc caagaggagc
      541 ccgcgaaggg ccagcccggg cgtccggatc gggccggata gcccggaaga tccccgcgaa
      601 gttcgcctgc gtacagaggg atgggccctg ccgcgtcgaa caccgcggta ggagatcggc
      661 aacaccggcg aaaggcctca ggcccacgcg aacggtgccg atacggaagg taaggagggt
      721 gagatgaacg gagtcgcgaa gcagttctgg agcgaggtca tggacatccg ctctcccctg
      781 ccggacgacg gccgcggccg gaaggtcgcc cgcaagggcc ggaagatcca gcgccggacc
      841 gtctccaccg cccgccgcac cggtcggccc gccgcctgac ccggacccgt ctgctaccat
      901 ccctcaggtc atggcagccg tgtgcgagat ctgcgggcgc aagccggggt tcgggatgca
      961 ggtgagtcac tcccaccgcc ggtccaaccg ccgctggtca ccgaacgtcc agaagatccg
     1021 ggtcctgatc aacggagccc cgaagcgcgc gcacgtctgc acgaagtgca tcaaggccgg
     1081 caaggtcacc cgcgcgatct gaggcgggcg ggccctagag ctcccgccgg gtccacgagc
     1141 aggtctcagc gaggcacgcg cgcacccaca gatcgtggtt cggctccggc tcggccagcg
     1201 tcgccgtgaa cctgcctgaa tcgagcgagg caggcgagaa gccgacctcc tcatacgggg
     1261 ccccctgcgg agccgtgccg accgcgaccg aaggcgcggc gagggtcgcc gccggaagcg
     1321 tcacctcgtc gagcgggagg aacgggtcac ccccggactc ggcgagcgcc gtatgcgcgc
     1381 ggagcgagag cggcgtcccg ggctcgaggc ccagccgatc gagtggaagc gcgacgcgca
     1441 ccgagtcgcc ggtgttaccg tagctcccgc gcagaccccc gacgcggacg cagtcgggtt
     1501 cgcaccgata cagatggaag gtcggaccga gcggcacgtc gttctccgtg ctccgcagga
     1561 cccgcgcttc gaaccgcacc ggccccgctg cgatctcgaa cccgtagcgg accgcgggga
     1621 ggccggccca cccggtcgcg ctgcctccgg ccgccggggc cgcggcggga cgcgcgccgg
     1681 ggatggcgct cacatcgatg cgcagcagca gatcctccgc ctcggggcgc aggacggcgg
     1741 atgccgccgt gatctcgcct ccgacccgcg gcgccgcgcc cgccccgtcg tcagcggggt
     1801 ccgccgcgct cgcgaggacg gcgccggaga aggacgccca gccgccgaca cgcacctgac
     1861 cgtcgacgga cccgagggag atcgcaccga cgcccaggtc cggaccgcgg tcgcgggcga
     1921 cgacctcgcg gtccccctcc tgcgtctccg gcaggaggtt ccacgcgtcg gtcgcgaaga
     1981 tcacctcacg acctccgcgg gcgaacgcgg gccgacccga cgcggaccag tggttgccct
     2041 cttcgccgct ctcggagacg gagacgcggt cgacgccgcc cgtgacgcgg tcgtggacga
     2101 aggtgtccat cgtctcgttg gtgtccccgg ggacgaggtt cgacgccatg ctgtggaagg
     2161 cgacgtaccg gccgttgccg gagatc
//
LOCUS       DATLGG010000213         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_229223, whole genome shotgun sequence.
ACCESSION   DATLGG010000213 DATLGG010000000
VERSION     DATLGG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229223"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VM638_06050"
     CDS             complement(<1..730)
                     /locus_tag="VM638_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVM12019.1"
                     /translation="MTSDPPARFRDELRPSILLPSLTQGLVLGILEVVVATSFAAFIF
                     SGPLAVHLPAGIGASLFAAVAVMGILAVTSSLPGAIGSVQDSTAAVMAIGAAAIASRL
                     PPEAPETFLTVILAVGVTTTAAGLFFLVLGTFRLGDLIRFVPYPVIGGFLAGTGWLIA
                     KGAVGVLTGIDVSLATLGELGTSAAAARWGPGLAFAVILLVLLRRTGRFLLIPGMVIG
                     GAVAFYVVLAATGTSIAAAESGGWL"
     gene            complement(774..2186)
                     /locus_tag="VM638_06055"
     CDS             complement(774..2186)
                     /locus_tag="VM638_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM12020.1"
                     /translation="MVPLRILITAVLAVAGMAPTAVPAASQTACLPLGVDGRCPAWVS
                     TEDGGDFDITTAVTTAPDGTAVYAAGTTVTPGGYRSLLVASDAAGGERRWAVTDGRDG
                     DAYDTAYALAVSPDGGSVYIAGGACGAIDDGSTCDVLVAAHDAETGATRWTALRDGTG
                     RGLDSAQGLALSRDGSVLFVGGQTFSAQTGLDYLLLAYDARSGDELWHATWDRARGGD
                     RASALALAGDAVVLTGRSGSDAEADIATVAFDTREEREGEILWAARYDGGGNLDEAFA
                     MAADARRVVVAGESRTTTPFSPVVVAYDAVTGERSWVDRHDDPGLLGGTAFDVTISRD
                     GRLVAATGYTRRGDAKGPTTTLYDASTGEPRWRAHLPDGVGQGVAISPDGRRVYTTGP
                     TYTRRGTMHRTVSYLAATGAERWSGLYEQAAPPHGLLDTLLYPSGIALSPDGTHVYVG
                     GATRGPLATHDDAMVLSYQA"
     gene            complement(2298..>2614)
                     /locus_tag="VM638_06060"
     CDS             complement(2298..>2614)
                     /locus_tag="VM638_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12021.1"
                     /translation="AITSDNAGPVSDPALGHVFWGKNAAVKTAEVVNNPSGGSQCVTI
                     RMRVTVPAGSTRYLLFFTEMSNSNGAAQTSISKFNTEGLSSSLLTGIKSSVRSKIVNW
                     NL"
BASE COUNT          385 a          946 c          946 g          337 t
ORIGIN      
        1 ggagccatcc gccgctctcc gccgccgcga tcgacgtccc cgtcgcggcg agcaccacgt
       61 agaacgcaac ggcccccccg atcaccatcc ccgggatcag caggaaccgg ccggtgcgcc
      121 ggaggagcac gaggaggatc accgcgaacg cgaggcccgg cccccaccga gccgcggcgg
      181 ccgaggtgcc gagctcaccg agcgtcgcga gcgagacgtc gatgccggtc agcacgccga
      241 ccgcaccctt cgcgatcaac caacccgtcc cggcgaggaa cccgccgatg acggggtacg
      301 gcacgaaccg gatcaggtcg ccgagacgga acgtcccgag cacgaggaag aagagcccgg
      361 ccgccgtcgt ggtcacgccc accgccaaga tgaccgtcag gaaggtctcc ggcgcctccg
      421 gcgggaggcg gctcgcgatc gccgccgccc cgatcgccat gaccgcggcc gtgctgtcct
      481 ggacgctgcc gatcgcgccg ggcaggctgc tcgtcaccgc gaggatcccc atcaccgcga
      541 cggccgcgaa gaggctcgcg ccgatcccgg ccggcaggtg gaccgcgagc ggaccggaga
      601 agatgaacgc ggcgaacgag gtcgccacga ccacctcgag gatcccgagc acgagcccct
      661 gggtgaggga cgggagcagg atgctcggcc ggagctcgtc gcggaagcgg gcgggcgggt
      721 cgctcgtcac ggacgtcatc ctggcacggc gcgcgcggcg gagggaacct cggctacgcc
      781 tggtacgaga ggaccatcgc gtcgtcgtgc gtggccagcg ggccgcgggt cgcgccgccg
      841 acgtagacgt gcgtcccgtc ggggctcaac gcgatccctg agggatacag cagggtgtcg
      901 agcagcccgt gcgggggtgc ggcctgctcg tagagcccgc tccagcgctc cgcccccgtc
      961 gcggcgaggt aggagaccgt tcggtgcatc gtgccgcggc gggtgtaggt cgggccagtg
     1021 gtgtagacgc ggcgtccgtc ggggctgatc gcgacgccct gcccgacgcc gtcgggaagg
     1081 tgtgcgcgcc aacgcggctc ccccgtcgac gcatcgtaga gcgtcgtcgt cgggcccttc
     1141 gcgtcgccgc gtcgcgtgta cccggtcgca gcgacgagcc tgccgtcccg gctgatcgtg
     1201 acatcgaagg cggtgccgcc caggaggccc gggtcgtcgt ggcggtcgac ccaggaccgc
     1261 tcccccgtca ccgcgtcgta ggcgacgacg acgggggaga aaggggtggt ggtgcggctc
     1321 tcgcccgcga cgacgacgcg ccgcgcatcg gccgccatgg cgaacgcctc atcgaggttg
     1381 ccgcccccgt cgtagcgcgc cgcccagagg atctcgccct cccgctcctc acgcgtgtcg
     1441 aaggcaacgg tcgcgatatc cgcctcagcg tccgagccac tgcggccggt gaggacgacg
     1501 gcgtcgccgg cgagcgcgag ggcagaagca cgatcgccgc cacgcgcgcg gtcccacgtg
     1561 gcgtgccaca gctcgtcgcc cgagcgggca tcgtaggcga gcaggaggta gtcgagaccg
     1621 gtctgcgccg agaacgtctg gccgccgacg aacaggaccg agccgtcgcg gctcagcgcg
     1681 agaccctgag cgctgtcgag gccgcgcccg gtcccgtcgc ggagggccgt ccagcgtgtc
     1741 gcgccggtct ccgcgtcgtg cgccgccacc aggacgtcgc acgtcgaccc gtcgtcgatc
     1801 gcgccgcagg caccaccggc gatgtagacc gatcccccgt cgggggagac cgccagcgca
     1861 tacgcggtgt cgtacgcgtc cccatcacgg ccgtcggtaa cggcccagcg acgctcgccc
     1921 ccggcggcgt cggaggcgac gaggagcgac cggtaccctc ccggcgtgac ggtggtcccg
     1981 gcggcgtaga cggcggtgcc gtccggagcc gtggtcaccg cggtcgtgat gtcgaagtcc
     2041 ccgccgtctt cggtcgaaac ccaggccggg catcgcccgt cgacaccgag cggcaggcag
     2101 gccgtctgag acgccgccgg gacagccgtg ggcgccatgc cggcgaccgc cagcaccgcg
     2161 gtgatgagga tcctgagggg caccatgccg agttgattcg ccgtccggcg cttgctcccc
     2221 tttgtgcagg tgcacgaagg taggcgcgga ggaggggggc cggatcgtcc ggcccccctc
     2281 ctgtgctgcc gcgcgagcta caggttccag ttcacgatct tgctgcgaac gctggacttg
     2341 atcccggtga ggagactgct gctcagtccc tccgtgttga acttcgagat gctcgtctgc
     2401 gccgcgccgt tcgagttgct catctcggtg aagaagagca ggtagcgcgt gctcccggcc
     2461 gggaccgtga cgcgcatccg gatcgtcacg cactggctcc ccccagaggg gttgttcacg
     2521 acctccgccg tcttcaccgc ggcgttcttc ccccagaaga cgtgtccgag ggccgggtca
     2581 gagacgggac ccgcgttgtc actcgtgatc gccc
//
LOCUS       DATLGG010000214         3540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_170952, whole genome shotgun sequence.
ACCESSION   DATLGG010000214 DATLGG010000000
VERSION     DATLGG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170952"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VM638_06065"
     CDS             <1..980
                     /locus_tag="VM638_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVM12022.1"
                     /translation="FVAGATGAIGQRLVPRLIARGHEVVGMTRSTTKAQRLAGQGVEP
                     VVADALDREAVMAALQRSAPEVVVHQLTALSTLSNIRRFVKEFEATNRLRTHGTDHLL
                     DAATAAGARRFVAQSYAGWPYARDGGPVKTEDDPLDADPPKAFRAAFGAIRHLERRVT
                     QAREIEGLVLRYGVFYGPGTGIAPGGAQLEEVRRRRFPVVGAGGGVWSFIHIDDAAEA
                     TVAAIEGGAPGVYNIVDDDPAPVSEWLPTLAAEAGAPPPRRAPAWLARVLAGEHGVVI
                     MTEARGASNAKAKRDLGWRPKHPSWRQGFADMLRSPHPEDENSAVSPST"
     gene            complement(1019..2464)
                     /locus_tag="VM638_06070"
     CDS             complement(1019..2464)
                     /locus_tag="VM638_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVM12023.1"
                     /translation="MFRLPARFAPVLLTAALITSVGGSPAAAAAGQLDTSFGNGGITS
                     PVDFGSADDVAQQSDGKLLALGGRLLVRFTPQGTLDSSFGSGGTVRLPFAENDELYQF
                     RIWYLPQVAVQRDDGVEKVLVLGRGNLGRLLIARFLPNGSPDRSFGENGVLTDDLPLA
                     GSTEMDLVTQPDGRIIVAATHPGPQVIVPNVCIPMGDCFGSVATGPGQGTLTRLLPDG
                     GRDTTFGVDGRTTLPGRGVVGEVLLQPDGKILVAAGRLYRLLPDGTPDLSFGVAGQAL
                     ASAQDIALGREIVAVSGDRASFSTISHDGRTVRDHALPSGIETWSLDVAAHPERIVIV
                     DQRRAVGFTWDGALDPSFGTGGYGPWIGMSHRTLLVQPDGKIVAGGGSGNPPGLSLAR
                     YLSDGPVFGSIAGSVTQQKAGSPIAGASVDCGDGLRATTGGDGRYSIANVPPGSRTCV
                     ASASGFDSATKQVSVKEGATAIADFVLRKGR"
     gene            2652..3155
                     /locus_tag="VM638_06075"
     CDS             2652..3155
                     /locus_tag="VM638_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM12024.1"
                     /translation="MTESEGASTTYHLGSFEEFFGSERDELFATLCLVTRDRHEAEEL
                     TQEAFVRVLERWERVSGLENPRGYLYRTAMNVFRSRYRRTLVAAKRALRITPPDDGIV
                     EVEERDTAMRALAPLSPRQRAAVVLTDLLGFPSDEAARMLGIRASTLRMHASRAHAAL
                     RKGMSHE"
     gene            3148..>3540
                     /locus_tag="VM638_06080"
     CDS             3148..>3540
                     /locus_tag="VM638_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12025.1"
                     /translation="MSEYRALLEQARRQFPPPDITVETIADRGRRRQRNRRAGAIVMA
                     LTLLAGALAGLKALGDIRPGPRPADLPSAPESWSRVWIEQTPAGEDGFARPQEVFVAG
                     SRLVAWGIRTPAVIRPGEPASDPAAWEST"
BASE COUNT          508 a         1260 c         1276 g          496 t
ORIGIN      
        1 tcttcgtggc gggcgcgacc ggcgcgatcg ggcagcgact cgttccccgt ctgatcgcca
       61 gagggcacga ggtcgtcggg atgaccaggt cgaccacgaa agcccagcgg ctggcggggc
      121 agggggtcga gccggtcgtc gccgacgccc tcgaccggga agcggtcatg gcggcgcttc
      181 agcggagcgc tcccgaggtg gtcgtccacc agctgacggc gctttcgacg ctgtccaaca
      241 tccggcggtt cgtgaaggag ttcgaggcga cgaaccgact gcggacccac gggaccgacc
      301 acctgctgga cgccgccacc gcggcgggcg cccggcggtt cgtggcccag agctacgcgg
      361 ggtggccgta cgcgcgcgat ggcgggccgg tgaagacgga ggatgaccca ctcgacgcgg
      421 atccaccgaa ggcgttccgt gccgcgttcg gtgcgatccg gcacctcgaa cgacgcgtga
      481 cgcaggcacg ggagatcgaa ggcctcgtgc tgcgctacgg cgtcttctac ggacccggca
      541 cggggatcgc cccgggcggc gcgcagctgg aggaggtgcg acggcggcgg ttcccggtgg
      601 tgggggcggg aggtggtgtg tggtccttca tccacatcga cgacgccgcg gaggcgacgg
      661 tcgcggcgat cgagggggga gcaccgggcg tgtacaacat cgtggacgac gaccccgcgc
      721 cggtctcgga gtggctgccg acgctggccg cggaagccgg cgctcctccc ccacggcgcg
      781 ccccggcgtg gctcgcacgc gtgctcgccg gcgagcacgg ggtcgtcatc atgacggagg
      841 cgaggggcgc ctcgaacgcg aaggcgaagc gggacctcgg ctggcgcccg aagcacccca
      901 gctggcgaca aggcttcgcc gacatgctga ggtccccgca cccagaggac gagaactccg
      961 cggtctcacc ctcgacgtag ccgggcggtc tcccggacgg gtgccgttcg gctcacgctc
     1021 agcggccctt ccggagcacg aagtccgcga tcgcggtcgc gccctccttc accgagacct
     1081 gcttcgtggc gctgtcgaag ccgctcgccg aagccacgca cgtgcggctt cccgggggca
     1141 cgttcgcgat cgagtagcgc ccgtcaccgc cggtcgtcgc gcggagccca tcgccgcagt
     1201 cgaccgatgc gccggcgatc ggcgagccgg ccttctgctg ggtcaccgac ccggcgatcg
     1261 agccgaagac cggtccgtcc gacaggtacc gggcgagcga caggccaggc gggttgccgg
     1321 acccgccgcc ggcgacgatc ttcccgtcgg gctggacgag cagggtgcgg tggctcatcc
     1381 cgatccacgg tccgtacccc cccgtgccga agctcgggtc cagggcgccg tcccacgtga
     1441 atccgacggc gcgccgctga tcgacgatga cgatccgttc cgggtgagcc gccacatcga
     1501 gggaccaggt ctcgatcccg ctgggcaacg cgtgatcccg caccgtccga ccgtcgtggc
     1561 tgatcgtgct gaaggacgcg cggtccccgc tgaccgccac gatctcccgc cccagcgcga
     1621 tgtcctgtgc ggacgcgagg gcctggccgg ccacgccgaa cgacaggtcg ggcgtcccgt
     1681 ctgggagcag acggtacaac cgtcccgcgg ccacgaggat cttgccgtcc ggctggagga
     1741 ggacctcgcc cacgaccccg cgcccgggca acgtcgtacg cccgtccacg ccgaacgtcg
     1801 tgtcgcggcc gccgtccggg agcagacgcg tcagggtgcc ctgtccgggc ccggtcgcga
     1861 cgctgccgaa gcaatcaccc atcgggatac agacgttggg gacgatgacc tgcggtccgg
     1921 ggtgggtcgc ggccacgatg atccgcccgt cgggctgcgt cacgagatcc atctcggtgc
     1981 tgccggcgag cggcaggtcg tcggtcagga cgccgttctc cccgaagctc ctgtccgggc
     2041 tcccgttcgg gaggaaccgg gcgatcagca accgtccgag gttccctcgt cccaggacga
     2101 gcaccttctc gaccccgtcg tcccgctgga ccgcgacctg aggtaggtac cagatccgga
     2161 actggtagag ctcgtcgttc tccgcgaacg gcagccgcac cgtcccgccg gagccgaagc
     2221 tcgagtcgag ggtcccttgc ggcgtgaagc ggaccaggag tctgccgcca agcgcaagga
     2281 gcttgccgtc ggactgctgg gccacgtcgt cggccgagcc gaagtcgacg ggcgaggtga
     2341 tcccgccgtt cccgaacgat gtgtcgagct gtcccgccgc ggcggcggcg ggcgatcccc
     2401 ccacggaggt gatgagggcg gccgtcagca ggacgggggc gaaccgggcg ggcaagcgga
     2461 acatcgaggc tctccttcgg tcggtttcct tatccgtacg gtgtgacatc cgccgccacg
     2521 atgtgtcgcc tctcccgtcc cgagctcggt ccgaccctcc aggtccccat cggcccgggt
     2581 tatctgctcc gggtgtaggc attctggcgc ttcctcgcgt tgatacttcg atgtacgaac
     2641 ctctgagagc ggtgacggag agcgagggag cgtcgacgac gtatcacctg ggctcgttcg
     2701 aggagttctt cggcagcgag cgggacgagc tcttcgccac gctctgcctc gtcacccggg
     2761 accgccacga ggcggaggag ctgacgcagg aggccttcgt gcgcgtgctc gaacggtggg
     2821 agcgcgtgag cgggttggag aacccccgcg ggtacctcta ccgaaccgcg atgaacgtgt
     2881 tccggagccg ctaccgccgg acgctggtcg ccgcgaagcg ggcgctgcgc atcacccctc
     2941 ccgacgacgg gatcgtcgag gtcgaagagc gcgacaccgc gatgcgggcg ctcgccccac
     3001 tctcgccccg ccagcgggcg gccgtcgtcc tgaccgatct gctcggcttc ccatcggacg
     3061 aggccgcacg gatgctcggg atccgggcct cgacgctgcg gatgcacgct tctcgggcgc
     3121 acgccgccct gaggaagggg atgtcccatg agtgagtacc gtgccctgct cgaacaggcc
     3181 cgccggcagt tcccgccccc cgacatcacc gtcgagacga tcgccgaccg cggtcgccgc
     3241 cggcagcgga accgacgggc gggagcgatc gtcatggcac tcaccctgtt ggcgggggcg
     3301 ctcgccgggc tcaaggccct cggtgacatc cggcccgggc ctcgccccgc cgacctgccc
     3361 agcgccccag agtcctggag ccgggtctgg atcgagcaga ccccggccgg cgaagacggg
     3421 ttcgcgcgcc cacaggaggt cttcgtcgcc ggctcgcgtc tcgtggcgtg ggggatcagg
     3481 acgcctgcgg tgatccggcc gggcgagccg gcctccgacc ccgccgcgtg ggagtccacc
//
LOCUS       DATLGG010000215         9287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_46068, whole genome shotgun sequence.
ACCESSION   DATLGG010000215 DATLGG010000000
VERSION     DATLGG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46068"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1160)
                     /locus_tag="VM638_06085"
     CDS             complement(138..1160)
                     /locus_tag="VM638_06085"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HVM12026.1"
                     /translation="MTLPFGPPLAPMLARSQDEIPEGAGWRYEPKWDGYRAIVFRDGP
                     DVHIGSRGEKPLHRYFPELLPVLRDALPKTCVLDGEIITPVNGVLEFDVLSQRIHPAA
                     SRIELLSRETPATFVAFDLLAAAGRDLRERPLRERWERLAKALADLPSDFDAVLSPGP
                     AVCLTPQGSDAVEAARWLEDLAASGLDGIIAKREEEPYRPGERTMVKVKRTKTADVVI
                     GGYRVHKSGEGVGSLLLGLYDDAGVLHHVGFSGAFPAKQRREMLEILRPLEGGDAFGP
                     GAGPGGPSRWRQEERAWVPLEPKLVAEVAYDHVTGQRFRHGTRLLRWRDDKPPEACTL
                     DQIRPG"
     gene            1212..1583
                     /locus_tag="VM638_06090"
     CDS             1212..1583
                     /locus_tag="VM638_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12027.1"
                     /translation="MDDNMVTSDEDVQRSGHRPADLVPPPDEPVGVDERTTAAEQREG
                     DTLGERLNRERPDRGAGAATLPAEDLVDDDPDHEKDLVAEEAESEGGREVTPEEGAMR
                     VVDEDEAPGLTDGPDRYVEEE"
     gene            1698..3803
                     /locus_tag="VM638_06095"
     CDS             1698..3803
                     /locus_tag="VM638_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12028.1"
                     /translation="MRSRRWRRAVVVAPLLVTLLATAASAYPRPGRTELVSLAHDGSL
                     GDRAWETASEWPALTPDAGLVAFHSSATNLVPADTNNRSDVFLRDRVHGTTTRISVAS
                     DGRQGNHDSYSPRITPDGRYVAFDSWASTLWPGDMGWSDVFVHDRQTGFTERISTPVG
                     GGNGNEGSWGPAISDDGRWVAFDSQATNMVAGDINGYSDVFLHDRQTGTTELISKSSQ
                     GIQGDTFSAYPAISADGRYVAYTSRATNLVLGDTNRTYDVFVYDRVEEDVERVSVASD
                     GTEGNRGGFNPVRMSADGRYVAFVSESSNLVSHDGNGTVWDVFVHDRETGRTRRVSAS
                     SAGEPANGFSMLTGFSRDGRYVTFWSDASNLVPGDTNDERDVFLHDLHAGTTEIVSVT
                     HNGAKANNMSWGIGDVTPGGRHVGFMSVASNIVPGDTNGGSDVFVRDRGPALGVLVMD
                     VVDDEAGRPLVEGSATFSGRPIASAADPAADAGHGIALGADLTRADLTYRPETGDLLV
                     RLRLDRLPTVSPCVDSGLCPAVGPPGVVYGLELTVDGVPHEIRAMRIAATGAAAETPL
                     IALYRCPGECSRVATLKGGIGTTETEVSAAVPLATLGADEGDELTGVRAFTSLGEVAT
                     GSLQEFDSVSVGSAEIPRRSVSVAFAPEGLPGAEVSFGDEASLDGARFSGRPSGAGER
                     AWARVCLGAECAFGSTLRP"
     gene            3952..4152
                     /locus_tag="VM638_06100"
     CDS             3952..4152
                     /locus_tag="VM638_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVM12029.1"
                     /translation="MATGTVKWFNPDKGFGFISQEDGPDVFVHYSAIEGSGYRNLEEN
                     QQVEFDITQGEKGPQASNVRPV"
     gene            complement(4211..6700)
                     /locus_tag="VM638_06105"
     CDS             complement(4211..6700)
                     /locus_tag="VM638_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM12030.1"
                     /translation="MSTRGRAALTVPLALILLGAPASAQEAPAEPELLFAQPQPRGTA
                     LLGTADLDDVPGEEAVLASATTLRAVKLDGSILWEVTTAAMTRPAIVPDMTGDGVVDV
                     AAQVAFPGRSGEVLLVDGATGGIHWREPVHSPVGSIVATDLNADGNLDLVTPSVDGTM
                     RIRAFFGPDLATGWTTTLPGTLNAYWVFAESMAAGDLDGVSGSEVVFGTRMGQNESGS
                     IHALDGATGTIRWTQPTGAIFGLKVASGRVVGLQWHPAVVTWRAELAAWPGGGGSPLW
                     THPLHGHVKPANLLIGDVDADGGLDVVTAATPFQEGTVLFGLDPSLGMVHAVGIEDGR
                     PRWASKVNREVSTLAAVPRAAGGIDVAYGTDAWITTYPDEQVGLLSGTSGAPIWVHVR
                     PSETQDDRIAGIAHADLDADGTREIVYGANEQKIVGLGLANGAVLFRQAYPAIWMAAD
                     AGDVDGDGRPESVGGGSEGVVQMLHADGSTAWATRVGGLVGGVRVQDDTVLVAALGAD
                     TRVHALDASNGGERWSFTTGVGATNNFSIHQQAFGDLDGDGVDDVVIGGAQNLRPTVV
                     AVNGRTGQLLWRSLSPLLPPGQQPLTWAGAVNSIAVIGDDVAAFIRPLDSREQATPRL
                     ALLNGVGGTPLWTATSSRGEVAAAIVPLGDQVLFAFGNRHVSARRLADGTESWTLDLG
                     NERHLVPLGADRVAAIARWSGVNRTFDATILTPSGTAGSVADPQLPAPVAAGLVPGAG
                     ATLATREGVMVLDAARLASREIRADRQVRLRRYTNPNNESIDIPSATRVFGIRLGGRS
                     FAFGTPDRLLLNGTGGEMPGVWAARLLPPRS"
     gene            complement(6697..8754)
                     /locus_tag="VM638_06110"
     CDS             complement(6697..8754)
                     /locus_tag="VM638_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12031.1"
                     /translation="MTTIRARSVALALAAGVVLTALPASAYPRPGRVTRISLAADGSQ
                     STGESYHAVVSADGGSLTFTSYGELLPEDRSGHADVYVKDLRTNELELVSVTPGGIAG
                     ENHSRWPSISADGRIVAFESEADDLVPGDTNAVTDVFVYDRGTGVVKRVSVTSDGAQV
                     AAWSRRPEVSADGRHVVFYSESEELDPNDVSPYIDVFVHDLQTGKTELVSVSTHGTQS
                     ILPASGADISGDGNIVAFSARYPLADPPDVNGIYEDVFIRDRAAGTTERISDSALGLQ
                     GESHSIYPSLSDDGRYVAFQSLASTLISNDSNRFADIYVYDRVTERLERVSVGTHGQE
                     GNHVAASGSISADGRYVVFVSGASNLSVEPDQNGVADVFVHDRLSGTTELLSRTPGGT
                     TGDHISHQPSISGDGRIAAFISYASDLVPDDTNGVLDIFTHDRGPELGTGEVVARRGS
                     ERIDVEGWARFAGRIVAQGTDFTGDAVPAGYADLAAASLVQRPEEGDLQFRVQLGALP
                     ETSPAGVGPKGLATISYSASFTVASVTYRVTVVPMPETDPLTPSFTLSRCVGPCTPIG
                     PVPGSFGGTMDGFVVSVPLATIGLTPPAAVSSSSVASHQTLPGSARTADTISLGGGTI
                     GAATVSAGIAPPGTPVEDVVFAPAPTGEGRFWTSFPAASGARDVWVRACLAGTCRTTK
                     AEA"
     gene            complement(8820..>9287)
                     /locus_tag="VM638_06115"
     CDS             complement(8820..>9287)
                     /locus_tag="VM638_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12032.1"
                     /translation="ATPELGARGGVRGEGPGWVDASRTFPGYGIAPLQPVRRDVTLAL
                     AQDTLTETNGGWIPVRARLLDGGAPVANREVTFDLEWRSESPLGDAWFPHHFRAHGVT
                     DADGWATAHLDADEFRDYWSGDPSWLTLRIAARYHGDPARRPASDARPITFSG"
BASE COUNT         1324 a         3255 c         3394 g         1314 t
ORIGIN      
        1 ggccccggcc gcgatcgcca gcacggcgag tgagagcgga aggatcttct tgcgcatcca
       61 cagccccttt cggagttagg tatgcctaac ccagttggtt aggccgtgcg aatactagtg
      121 atgcggtcgg attcgcgtca acccggacgg atctggtcga gcgtgcaggc ctcgggcggc
      181 ttgtcgtcgc gccagcggag caggcgcgtg ccgtgacgga accgctggcc cgtcacgtgg
      241 tcgtaggcga cctcagcgac gagcttcggc tcgagcggga cccacgcccg ctcctcctgt
      301 cgccagcggc tcggcccgcc gggccccgct cccgggccga acgcgtcgcc tccctcgagc
      361 ggccggagga tctcgagcat ctcgcgccgc tgcttcgccg ggaacgcccc cgagaacccg
      421 acgtgatgca ggacccctgc gtcgtcgtag aggccgagga gcagggagcc aaccccctcg
      481 ccgctcttgt ggacgcggta cccgccgatc acgacgtccg cggtcttggt ccgcttgacc
      541 ttcaccatcg tgcgctcgcc gggccggtag ggctcttctt cccgcttggc gatgatgccg
      601 tcgagcccgg aggctgccag gtcctccagc cagcgcgcgg cctcgacggc gtccgagccc
      661 tggggcgtca ggcagacagc cgggcccggg gagaggaccg cgtcgaagtc cgacggcagg
      721 tcggcgagcg ccttcgccag ccgttcccac cgctcgcgga gggggcgctc gcggaggtcg
      781 cggccggccg ccgcgaggag gtcgaaggcc acgaaggtgg ccggcgtctc ccgtgacagc
      841 agctcgatgc gggaggccgc cggatggatc cgctgcgaga ggacgtcgaa ctcgaggacc
      901 ccgttcaccg gcgtgatgat ctcgccgtcc aggacgcagg tcttcggcag ggcgtcgcgc
      961 agaacgggca ggagctctgg gaagtagcgg tggagcggct tctcaccgcg gctcccgatg
     1021 tggacgtcgg gcccgtcgcg gaacacgatc gcccgatagc cgtcccactt cggctcgtag
     1081 cgccacccgg cgccctcggg gatctcgtcc tgggatctcg cgagcatcgg ggcgagcggc
     1141 ggcccgaacg gcagcgtcat gcgacccatc atcgccgttt gtgcgctgcc gcgtccgggt
     1201 agggcccgcc catggacgac aacatggtga cgagtgatga ggacgtgcag cgctccggcc
     1261 accggccggc cgacctggtt cccccgcccg acgagcccgt tggcgtcgac gagcggacga
     1321 ccgcagccga gcagcgcgag ggggacaccc tcggcgagcg cctgaaccgc gagcggcccg
     1381 accgcggcgc cggtgccgcc acgcttcccg ccgaggacct cgtggacgac gatccggacc
     1441 acgagaagga tctcgtcgcc gaggaggcgg agtccgaagg cggccgcgag gtgacccccg
     1501 aggaaggggc gatgcgcgtc gtcgacgagg acgaagctcc gggtctcacg gacggccccg
     1561 accgctacgt cgaggaggag tagcgcggcg ggcggcccgc cgccccgtga cgggacttcc
     1621 tgtgcacctg cccaaagggt tcgcgcgcgc acgccgcgaa caggggaagc ggcagggggg
     1681 agaacaggag gttccacatg cgttcgcgtc gttggcggcg cgcggtcgtg gtcgcgccgc
     1741 tcctcgttac ccttctcgcc accgcggcat ccgcttaccc gcggccaggg cggaccgagc
     1801 tggtctcgct cgcacacgac ggcagcctcg gcgaccgcgc ctgggagacg gccagcgagt
     1861 ggccggctct gacacctgac gctggactgg tcgccttcca cagctcggcg acgaacctcg
     1921 ttcccgcgga caccaacaac cgctccgacg tcttcctccg cgatcgggtc cacgggacga
     1981 cgactcggat ctccgtcgcg agcgacggcc gccagggcaa ccacgactcc tactcgcccc
     2041 ggatcacgcc cgacgggcga tacgtcgcct tcgacagctg ggcgtcgacg ttgtggccgg
     2101 gcgacatggg ttggtcggac gtgttcgtgc acgatcggca gacagggttc accgaacgga
     2161 tctcgacccc ggtcggtggc gggaacggca acgagggttc gtggggcccc gcgatcagcg
     2221 acgacggccg ctgggtcgcc ttcgacagtc aggcgacgaa catggtggcc ggagacatca
     2281 acggctactc cgacgtgttc ctccacgacc gacagaccgg cacgaccgag ctgatctcca
     2341 agtcgagcca ggggatccag ggcgacacgt tctcggccta tcccgcgatc agcgccgacg
     2401 gccgctacgt cgcctacacg agccgtgcga cgaacctcgt gctgggcgac acgaaccgga
     2461 cctacgacgt cttcgtctac gaccgcgtcg aggaggacgt cgagcgggtc tccgtcgcga
     2521 gcgatggcac ggaggggaac cgtggggggt tcaacccggt gcggatgagc gccgacggcc
     2581 gctacgtcgc cttcgtgtcg gagtcgagca acctcgtgtc ccacgacggg aacggaacgg
     2641 tctgggacgt gttcgtccac gaccgcgaaa ccggccgcac gcgccgtgtg agcgcctcga
     2701 gcgccggcga gccggcgaac ggcttctcca tgctcaccgg gttcagccgt gacggccgct
     2761 acgtgacgtt ctggagcgac gcctcgaacc tcgtccccgg ggacacgaac gacgagcgcg
     2821 atgtcttcct gcacgatctc cacgcgggca ccaccgagat cgtctcggtg acgcacaacg
     2881 gcgcgaaggc gaacaacatg tcctggggga tcggcgacgt gactcccggc ggacgccacg
     2941 tcgggttcat gagcgtcgcc tcgaacatcg tgcccgggga cacgaacggc ggctccgacg
     3001 tcttcgtccg ggaccggggc cccgcgctcg gtgtgctggt gatggatgtc gtcgacgacg
     3061 aggccgggag gccgctcgtc gagggctcgg cgacgttctc ggggcggccg atcgcctcgg
     3121 ccgcggaccc tgccgccgac gccggtcacg ggatcgcgct cggcgcggac ctgacgaggg
     3181 cggatctgac gtatcggccg gagacggggg atctgctggt ccgcctccgt ctcgatcggc
     3241 tcccgacggt ttccccgtgc gtggacagcg gcctctgccc ggcggtgggt ccgccggggg
     3301 tcgtgtacgg gctcgagctg acggtggacg gggtcccgca cgagatccgg gcgatgcgga
     3361 tcgccgcgac cggcgctgcg gccgagaccc cgctcatcgc gctctaccgc tgcccgggcg
     3421 aatgctccag ggtcgcgacg ctgaagggcg gcatcgggac gacggagacc gaggtctccg
     3481 ctgcggtccc gctcgccacc ctcggcgcgg acgagggcga tgagctgacg ggggtgcggg
     3541 ccttcacgtc cctcggcgag gtggcgacgg gctcgctgca ggagttcgac agcgtatccg
     3601 tcggatccgc cgagatcccg cggcggtcgg tctcggtcgc gttcgcaccg gaaggtctcc
     3661 ccggggcgga ggtgtccttc ggggacgagg cgtcgctgga cggcgcccgc ttctccggtc
     3721 gtccgtccgg cgccggcgag cgagcctggg cgcgcgtctg cctcggcgcg gagtgcgcgt
     3781 tcgggtcgac actgcggccg tagccttccc gctggtgtcc gtggcgccgc tcctgctagg
     3841 ctatgcgcga gggttcggcg ttatgagcag ttccctcacc ggcgttcgcg ccgggcggcc
     3901 gacggacgag caacaagcgt ccgatccggc caagaaagga ggggagtgcg tatggcgacc
     3961 ggtaccgtga agtggttcaa ccccgacaag gggttcggct tcatctccca ggaggacggc
     4021 cccgacgtgt tcgtgcacta cagcgcgatc gaggggtccg gctaccgcaa cctggaggag
     4081 aaccagcagg tcgagttcga catcactcag ggcgagaagg gcccgcaggc gtcgaacgtc
     4141 cggccagtct gaggtcgagg tactgaggga gcccgccgcg tgatcgcggc gggcttccgc
     4201 cttcccgggg tcagctcctc ggcggcagga gacgcgcggc ccacaccccg ggcatctcgc
     4261 caccggtccc gttcagcagg aggcgatcgg gggtgccgaa cgcgaagctg cggccgccga
     4321 ggcggatgcc gaatacccgg gtggccgagg ggatgtcgat ggactcgttg ttcgggttcg
     4381 tgtagcgccg cagccgcacc tgccggtccg cgcggatctc ccgcgaggcg agccgtgcgg
     4441 cgtcgaggac catcacgccc tcccgtgtgg cgagggtcgc accggccccg gggacgagcc
     4501 ccgccgcgac cggcgccggg agctgtggat cggcgaccga tccggcggtg ccgctcggcg
     4561 tgaggatcgt cgcgtcgaag gttcggttca ctcccgacca gcgagcgatg gccgcgacgc
     4621 ggtccgctcc gagggggacg aggtgccgct cgttcccgag gtcgagcgtc caggactcgg
     4681 ttccgtccgc gaggcgccgc gcggacacgt gacggttgcc gaaggcgaac agcacctggt
     4741 cgccgagggg gacgatcgcg gccgcgacct cgccgcgcga ggacgtcgcg gtccacagcg
     4801 gggtcccgcc gaccccgttc agcagcgcga ggcgcggtgt ggcctgctcg cgtgagtcga
     4861 gcggccggat gaacgccgcg acgtcgtccc cgatcaccgc gatgctgttg acggcgccgg
     4921 cccacgtgag cggctgttgc ccgggtggga gcagcggcga gagcgaccgc cacaggagct
     4981 ggccggtgcg cccgttgacg gcgaccacgg tggggcggag gttctgggct ccaccgatca
     5041 cgacgtcgtc gacgccgtcc ccgtcgaggt cgccgaaggc ctgctgatgg atcgagaagt
     5101 tgttcgtcgc accgacgccg gtcgtgaagc tccagcgttc gccgccgttc gacgcatcga
     5161 gcgcgtggac gcgcgtgtcg gcaccgaggg ctgcgacaag gacggtgtcg tcctggaccc
     5221 gcacgccgcc gacgaggccg ccgacccgcg tcgcccacgc cgtcgatccg tcggcgtgga
     5281 gcatctggac gaccccttca gagccaccgc cgaccgactc cggcctgccg tcgccatcca
     5341 cgtcgcccgc gtcggcggcc atccagatcg ccggataggc ctgtcggaac aggaccgccc
     5401 cgttggccag gccgagcccg acgatcttct gctcgttcgc gccgtagacg atctcccgcg
     5461 tgccgtcggc gtcgaggtcg gcatgcgcga tcccagcgat ccgatcgtcc tgcgtctccg
     5521 acggccggac gtggacccag atcggtgcgc cgctggttcc ggagagcagc ccgacctgct
     5581 cgtcgggata ggtcgtgatc cacgcgtcgg tcccgtaggc gacgtcgatg ccacccgcgg
     5641 cgcgcgggac cgccgcgagg gtcgagacct cgcggttcac cttcgaggcc cagcgcggcc
     5701 gtccatcctc gatgccgacg gcgtggacca tcccgaggct cgggtcgagt ccgaacagga
     5761 ccgtcccctc ctggaacggc gtggccgccg tcacgacgtc gagcccaccg tccgcgtcga
     5821 cgtcgccgat cagcaggttc gccggcttca cgtggccgtg cagcggatgg gtccagagcg
     5881 gggaaccccc gccgccaggc caggcggcca gctcggcgcg ccacgtcacg accgcggggt
     5941 gccactggag gccgacgacc cgcccggacg cgaccttcag gccgaagatc gcgccggtcg
     6001 gctgcgtcca gcggatcgtc ccggtcgcgc cgtcgagcgc gtggatcgag ccgctctcgt
     6061 tctggcccat ccgcgtgccg aagacgacct cgctgccgct gacgccgtcg aggtcgccgg
     6121 cggccatgct ctcggcgaac acccagtagg cgttcagggt gcccggaagg gtcgtcgtcc
     6181 agccggtcgc caggtcgggg ccgaagaacg cacggatgcg catcgtcccg tcgaccgagg
     6241 gcgtcacgag gtcgaggttg ccgtcggcgt tcaggtcggt cgcgacgatc gagccaaccg
     6301 gcgaatgcac gggctcccgc cagtggatcc caccggtggc gccgtcgacg agcaggacct
     6361 cgccgctacg acccgggaag gccacctgcg cggccacgtc gacgaccccg tcgccggtca
     6421 tgtccggcac gatcgccggt cgcgtcatgg cggcggtcgt gacctcccac aggatcgagc
     6481 cgtcgagctt gaccgctcgg agcgtggtcg ctgaagcgag cacggcctcc tcgccaggga
     6541 cgtcgtcgag atccgccgtc ccgaggagcg ccgtccccct cggttgcggc tgggcgaaca
     6601 ggagctccgg ctccgccggc gcctcctgcg cggacgcggg cgcaccgagg aggatcaggg
     6661 ccagcggaac ggtgagcgcg gctcgaccgc gagtgctcac gcctcggcct tcgtggtgcg
     6721 acaggtcccg gcgaggcagg cgcggaccca cacgtctcgc gctcccgacg cggctgggaa
     6781 cgacgtccag aaccgtccct cgcccgtcgg ggccggcgcg aagacgacat cctcgacggg
     6841 ggttcccggc ggtgcgatgc cggcgctgac ggtcgcggct ccgatcgtcc ctccgccgag
     6901 cgagatcgtg tccgcggtgc gcgccgagcc ggggagggtc tgatgcgagg cgaccgagct
     6961 gctggagacc gccgccggcg gggtgaggcc gatcgtggcg agcggcacag aaaccacgaa
     7021 cccgtccatc gtcccgccga acgagcccgg aacgggaccg atcggggtgc agggaccgac
     7081 gcatcgggag agcgtgaagc tcggggtcag cggatcggtc tcgggcatgg gtacgacggt
     7141 cacgcggtag gtcacggatg ccaccgtgaa gctcgcgctg tacgagatcg tcgcgagccc
     7201 cttcgggccg acgcctgccg gcgaggtctc cggaagcgcg ccgagctgga cgcggaactg
     7261 gaggtctccc tcctccgggc gctggacgag gctcgcagcg gcgaggtcgg cgtagcccgc
     7321 ggggaccgcg tcaccggtga agtccgtccc ctgcgcgacg atgcggccag cgaaccgcgc
     7381 ccatccctcc acgtcgatcc gctccgagcc ccgcctggca acgacctcgc ccgttcccag
     7441 ctccgggcct cggtcgtggg tgaagatgtc gagcacgccg ttcgtgtcgt cgggcacgag
     7501 gtcggaggcg tagctgatga acgcggcgat ccgcccgtcg ccgctgatgg atggctgatg
     7561 ggagatgtgg tctccggtcg tgccgcccgg ggtccgggag aggagctccg tcgtgccgct
     7621 cagccggtcg tggacgaaga catcggcgac gccgttctga tccggctcca ccgagaggtt
     7681 cgaggcgccg ctgacgaaga ccacgtaccg accgtcggcg ctgatcgacc ccgacgccgc
     7741 gacgtggttc ccctcttgac cgtgcgtgcc gacggagacg cgctcgagcc gctccgtgac
     7801 ccggtcgtag acgtagatgt cggcgaagcg gttggagtcg ttcgagatga gcgtcgaggc
     7861 gaggctctgg aaggccacgt accggccgtc gtcgctgagc gatgggtaga tcgagtggct
     7921 ctcaccctgg agcccgaggg cggaatcgga gatgcgctcg gtcgtccccg ccgcgcggtc
     7981 gcggatgaag acatcctcgt agataccgtt gacgtccggc gggtcggcca gagggtaccg
     8041 cgcggagaag gccacgatgt tcccgtcccc gctgatgtcg gctccggacg ccgggaggat
     8101 cgactgcgtc ccgtgggtgc tgacgctcac cagctcggtc ttcccggtct gcaggtcgtg
     8161 gacgaagacg tcgatgtagg ggctcacatc attggggtcc agctcctcgg actcgctgta
     8221 gaacacgacg tgccggccgt ccgcactgac ctctggccgg cgggaccacg cagcgacctg
     8281 cgccccgtcg gacgtcacgg acacccgctt cacgaccccc gttccgcggt cgtacacgaa
     8341 gacgtctgtc acggcgttcg tgtccccggg gacgaggtcg tccgcctccg actcgaaggc
     8401 gacgatgcgc ccgtcggccg agatggacgg ccagcgcgag tggttctccc ccgcgatccc
     8461 gccgggggtg accgagacga gctcgagctc gttcgtccgg aggtccttca cgtagacgtc
     8521 ggcgtggccg ctccggtcct ccggcaggag ctccccgtag ctcgtgaagg tcagggatcc
     8581 cccgtcggct gacacgacgg cgtggtacga ctctccggtc gactgggaac cgtcggccgc
     8641 gaggctgatg cgggtcaccc ggccgggccg cgggtacgcg gaggccggaa gggcggtgag
     8701 gacgacgccg gccgcgaggg cgagcgccac ggaacgtgcg cggatggttg tcatgctccg
     8761 atgattcgcg cccgggcacc ctgcttcctt tgtgcaggtg accaaagcct ccggaacggc
     8821 tagccgctga acgtgatcgg gcgcgcgtcg ctcgccggtc gccgggcggg gtccccgtgg
     8881 tagcgtgcgg cgatccggag cgtcagccat gacgggtccc cgctccagta gtcgcggaac
     8941 tcgtcggcgt ccaggtgcgc cgtggcccag ccgtccgcgt cggtgacccc gtgggcgcgg
     9001 aagtggtgcg ggaaccacgc gtcgccgagc gggctctcgg accgccattc caggtcgaag
     9061 gtgacctccc ggttcgcgac gggagcgccg ccgtcgagga gcctcgcgcg caccgggatc
     9121 cagccgccgt tcgtctccgt cagggtgtcc tgggccagcg cgagcgtcac gtcccgcctc
     9181 accggctgca gcggcgcgat gccgtagccg gggaacgtcc gagaggcgtc gacccagccg
     9241 ggtccctctc cccggacgcc gccgcgcgcg ccgagctccg gcgtcgc
//
LOCUS       DATLGG010000216         6311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_139466, whole genome shotgun sequence.
ACCESSION   DATLGG010000216 DATLGG010000000
VERSION     DATLGG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139466"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VM638_06120"
     CDS             <1..941
                     /locus_tag="VM638_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12033.1"
                     /translation="VPLEATMVTVTSRGLGPPYRRFAHRVRKRLARPGVRASLDPFLY
                     GTTMATLTPERVRRELDIWQSFEQAFVVGHASGIHWIADQIRRQGIQLRRPPVAIATN
                     ADMLDPHAHAAIEATFGVRVHTRYGSREMNRIATSLPGTIDRYAFNPFLVYAEVVDDA
                     GRPVPPGVPGRLALTDLNNLVMPLIRYDLGDIAIASDEIAGGFPVIEQILGRSSEVLT
                     LPSGKVLATVTIGNVLFLEQDMGSWVRFYQCAQTAENELELRVVWEDEPPPGLRERMV
                     ELLGSAADPDTVIRVRDVDDVGRLPSGKVWVVRREF"
     gene            complement(951..2078)
                     /locus_tag="VM638_06125"
     CDS             complement(951..2078)
                     /locus_tag="VM638_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVM12034.1"
                     /translation="MFGNSWRVGRVAGIEIRIDQSWILIALLITYSLYLRFTFIYREL
                     STPAAVILAVAAALLFFGSVLTHEMAHSLMAVRRKIPVRGITLFLFGGATHAKVESRG
                     PWDEFVISVVGPLTSVALALVFGAVGIWGDGVLPAPVAGMFGYLGWVNLLLAIFNLVP
                     GFPLDGGRVLRSAVWKATGSLQTATRVASFAGQVVGYLLVGVGIWSVFAGSLAGGIWF
                     AFIGWFLAQAAANSYEQMQIQRMLEKVDAESVMARDLVAVAPDLTLQEVVDRFFMRYD
                     HSAFPVEEGGRTLGLVTLRAVKRLPRERWGEARVREAMGAVDDQVTVTPGTGMDVVLT
                     KLQDGEVHRVLVVEGEEVVGIITPHDVARWFRRREEIDARS"
     gene            2114..3331
                     /locus_tag="VM638_06130"
     CDS             2114..3331
                     /locus_tag="VM638_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVM12035.1"
                     /translation="MSDAAEGAGTEVEALRRQLQAAEEREAAVSELLESIARSTFDLD
                     SLLQTVIDRAVRLCEADTGNIARHDGDVFRVVAFTRSPADYEELVRRRVYVPERGSVI
                     GRTLLERRVVQIEDVLQDAEYALTELQAVGGYRSLLAAPMLRDGEAIGAIAVARNEVR
                     PFTDAEVRLLSTFADEVVLAIETIRLFRTVEHQREELARYAPEVATLLATEEGQRLLA
                     GHRREITALFSDLRGFTAFAETAEPEEVLGVLRDYYAAAGEVITANGGSVEQFAGDGL
                     MAFFNDPVPAQDHELAAVRSAVEMRERFDALAERWRKRGYELGLGVGVASGYATLGRV
                     GFEGRYDYAAIGTVVNLAARLSDVAGPGEILLSQPVHAAVEDHVRTEPPRELHLKGFS
                     RSTVAFQVIGLGA"
     gene            complement(3350..3422)
                     /locus_tag="VM638_06135"
     tRNA            complement(3350..3422)
                     /locus_tag="VM638_06135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3387..3389),aa:Ala,seq:ggc)
     gene            complement(3449..5413)
                     /locus_tag="VM638_06140"
     CDS             complement(3449..5413)
                     /locus_tag="VM638_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVM12036.1"
                     /translation="MTDAPSLEEDPVAAGRAAIDRHAWSEGYDLLSRADREGSLAPED
                     IAALGEAAWWAGHIGEAISARERAYAAYLDASQPEEAAMFALILARDHLNRADGAIAG
                     AWLSRAERLLEGLPESRAHGWLLRARARAAAEKDGDHERALGLCEEALDLATRLGDRD
                     LLALALQGKGDALVALGRVEEGRSLQDEATVAAVSGELSPFVTGVVYCNVINACARLG
                     DYGRAGEWTEAAKRWCERQSISGFPGVCRVHRAEIMRLRGSWADAIEEVERACVELES
                     HGLLTHAGAAFDELGNMRLRMGDVAGATSAFRQAHELGREPQPGLALLLLAEGKIEQA
                     RTSLDRALEELSAEDRLRRAKLLPARARVALAGGDVDRACEMSEELSATADLYETTAF
                     RAQALTTRGSVEDAEGRHEEAIATLRQARRLWSEIDLPYEAAETRALLGEAYRAAGDE
                     GSATLELEASRATFERLGAPLDAARVTKLLEGVREEVAERVHPTFMFTDIVGSTNLVA
                     AMGDEAWEQILAWHDRTLRGAFEAHRGEEVDHTGDGFFVAFETQEEALACAVDIQRRL
                     AEHRKEAGFAPRIRIGVHATEASKVDGDYRGRGVHEAARVGAIGGADEIVASLSTVAT
                     TGRRFTASEPRRVELKGIPEPIDVVTVDWR"
     gene            complement(5449..5814)
                     /gene="rsfS"
                     /locus_tag="VM638_06145"
     CDS             complement(5449..5814)
                     /gene="rsfS"
                     /locus_tag="VM638_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HVM12037.1"
                     /translation="MTSSRDLAIVAARSASSKKAEDIVVMDVRSLIGITDFFVLASGT
                     NDRQVRTIAEEVQRALRNQDVKPVRREGEREGRWVLLDYVDIVVHVFHAEEREFYSLE
                     RLWADAPVLPWQDRAASSG"
     gene            complement(5811..>6311)
                     /locus_tag="VM638_06150"
     CDS             complement(5811..>6311)
                     /locus_tag="VM638_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HVM12038.1"
                     /translation="VLDGLLAATAARPRVLAGALPRDAARIVTSAGVGGARAIDLPVV
                     EAGSGLVRAEAEAVAALARERFARTARTAEPVRLVVLNGNGIPGIGERVARKLVPAGF
                     RLVSSQNASTFDVKETLIAATDERFLDEARLARELLGVGRVALDPQSSRVAEVTVIVG
                     RDFRAA"
BASE COUNT          917 a         2376 c         2141 g          877 t
ORIGIN      
        1 gcgtgcccct ggaggcgacg atggtgaccg tcacctcgcg cgggctcggc cccccgtacc
       61 gccgcttcgc ccatcgagtc cgcaagcggc tcgcgcgacc cggggtgcgc gcctcgctcg
      121 atccgttcct gtacgggacg accatggcga ccctgacgcc cgagcgcgtc cgccgggagc
      181 tcgacatctg gcagtccttc gagcaggcgt tcgtcgtcgg gcacgcgagc gggatccatt
      241 ggatcgccga ccagatccgc cggcaaggca tccagctccg caggccaccg gtcgccatcg
      301 cgacgaacgc cgacatgctc gacccgcacg cccatgcggc gatcgaggcg acgttcggcg
      361 tgcgcgtcca cacgcgctac gggagccgcg agatgaaccg catcgcaacg agcctgcccg
      421 gcacgatcga ccggtacgcg ttcaacccgt tcctcgtgta tgcggaggtg gtcgacgacg
      481 cgggccggcc ggttccgccg ggcgtgcctg ggcggctcgc gctgacggac ctcaacaacc
      541 tcgtgatgcc cctgatccgc tacgacctcg gggacatcgc gatcgcctcg gacgagatcg
      601 cggggggctt cccggtgatc gagcagatcc tcggacggag ctcggaggtc ctcacccttc
      661 cgagcggcaa ggtcctggcg accgtcacga tcggcaacgt cctgttcctc gagcaggaca
      721 tgggcagctg ggtccggttc taccaatgcg cgcagaccgc ggagaacgag ctcgagctgc
      781 gggtcgtgtg ggaggacgag ccgccgccgg gactccggga acggatggtg gagctcctgg
      841 ggagcgccgc ggatcccgac accgtcatcc gcgtccgcga cgtcgacgac gtgggtcgcc
      901 tcccgtcggg gaaggtctgg gtcgtccggc gcgagttctg agcggggccg ctagctccgc
      961 gcgtcgatct cctcacggcg gcggaaccac cgcgcgacgt cgtgcggcgt gatgatcccg
     1021 acgacctctt cgccctcgac gacgagcacc cggtggacct cgccgtcctg cagcttcgtg
     1081 agcaccacgt ccatgccggt cccgggcgtg accgtcacct gatcgtcgac cgcgcccatc
     1141 gcctcgcgca cgcgtgcctc gccccagcgc tcgcgcggaa ggcgcttcac ggctcgcagc
     1201 gtgacgagcc cgagcgttcg cccgccctcc tcgacgggga acgcgctgtg gtcgtaccgc
     1261 atgaagaatc ggtcgaccac ctcctgaagc gtcaggtccg gggcgaccgc gaccaggtcg
     1321 cgcgccatga cgctctcggc gtcgaccttc tccagcatcc gctggatctg catctgctcg
     1381 taggagttcg ccgcagcctg cgccaagaac cagccgatga acgcgaacca gatcccgccg
     1441 gcgagcgagc ccgcgaacac ggaccagatc ccgaccccga cgaggaggta gccgacgacc
     1501 tgcccggcga agctcgcgac ccgcgtcgcc gtctgcaagc tgccggtcgc cttccacacc
     1561 gccgagcgca gcacgcggcc cccgtcgagc gggaagcccg ggacgaggtt gaagatcgcg
     1621 agcaggaggt tcacccagcc gaggtagccg aacatcccag cgacgggcgc gggcaggacg
     1681 ccgtcccccc agatcccgac cgcgccgaag acgagcgcca gtgccacgct cgtgagcggc
     1741 ccgacgacgc tgatcacgaa ctcgtcccac ggtccgcgcg actcgacctt cgcgtgggtg
     1801 gcgccgccga acaggaacag cgtgatcccc cgaaccggga tcttccgccg gacggccatc
     1861 aacgagtgcg ccatctcgtg ggtgaggacc gagccgaaga acagcagggc cgccgcgacc
     1921 gcgaggatca ccgccgccgg cgtgctgagc tcgcggtaga tgaacgtgaa cctgaggtag
     1981 aggctgtagg tgatcagcag cgcgatcagg atccacgact ggtcgatccg gatctcgatc
     2041 ccggccaccc ggcctacccg ccacgaattc ccgaacatgg agcgagcata cccaccaccg
     2101 tagaatcgcc gcgatgagcg acgccgccga gggagcaggg acggaggtcg aggcgctccg
     2161 gcggcagctc caggccgccg aggagcgcga ggccgccgtc agcgagctcc tcgagtcgat
     2221 cgctcgctct accttcgacc tcgattcgct gctgcaaacg gtgatcgacc gcgccgtccg
     2281 cctctgcgag gcggacaccg gcaacatcgc ccgtcacgac ggcgacgtct tccgcgtcgt
     2341 ggcgttcacc cgctcccccg ccgattacga ggagttggtc cgccgccgcg tctacgtgcc
     2401 ggagcgcggg tcggtgatcg ggcggaccct gctggagcgg cgcgtcgtcc agatcgagga
     2461 cgtcctgcag gatgccgagt acgccctcac cgagctgcag gcggtgggtg gctaccgttc
     2521 gctgctggca gcgccgatgc ttcgcgatgg cgaggcgatc ggggcgatcg ccgtcgcgag
     2581 gaacgaggtg cgtccgttca ccgacgccga ggtgcgcctg ctcagcacgt tcgccgacga
     2641 ggtcgtcctc gcgatcgaga cgatccgcct gttccgcacc gtcgagcacc agcgggagga
     2701 gctggcgcgc tacgcccccg aggtcgcgac cctgctcgcg acggaggagg gccagcggct
     2761 gctcgcggga caccgccgcg agatcaccgc gctcttctcg gacctccgcg gcttcaccgc
     2821 gttcgccgag accgcggagc cggaggaggt cctcggcgtc ctgcgggatt actacgcggc
     2881 cgccggcgag gtcatcacgg cgaatggcgg atccgtcgag cagttcgcgg gagatgggtt
     2941 gatggccttc ttcaacgacc ccgtgcccgc ccaggaccac gagctggcgg cggtgcggag
     3001 cgcggtggag atgcgcgagc gcttcgacgc cctcgccgag cgatggcgga agcgcgggta
     3061 cgagctcggg cttggggtgg gcgtcgcgtc cgggtacgcg acgctcggcc gcgtggggtt
     3121 cgagggccgc tacgactacg ccgcgatcgg cacggtcgtg aacctcgcgg cgcgactgag
     3181 cgacgtcgcg ggaccgggtg agatcctcct gagccagccc gtgcacgccg ccgtcgagga
     3241 ccacgtgcgc acggagccac cccgcgagct ccacctcaag ggattcagcc gctcgaccgt
     3301 cgccttccag gtcatcggcc tgggcgcctg accgtcgaag ggagtgaggt ggagctgagg
     3361 ggattcgaac ccctgacctc ctccatgcca tggaggcgcg ctaccaactg cgccacagcc
     3421 ccgcggggcc gattctagca ggcggatttc agcgccagtc gacggtgacg acgtcgatgg
     3481 gctcggggat ccctttcagc tcgacgcgcc gcggctcgct cgcggtgaag cgcctgccgg
     3541 tcgtcgcgac cgtcgacagg cttgcgacga tctcgtccgc gccgccgatc gcgccgaccc
     3601 gagcggcctc gtggacgccc cttccccggt agtccccatc gaccttcgag gcctcggtcg
     3661 cgtggacccc gatgcggatc cgcggggcga accccgcttc cttccggtgc tcggcgagcc
     3721 ggcgctggat gtcgacggca caggcgagcg cctcctcctg cgtctcgaac gcgacgaaga
     3781 acccgtcgcc ggtgtggtcc acctcctcgc cgcggtgcgc ctcgaatgcc ccccggagcg
     3841 tgcggtcgtg ccacgccagg atctgctccc aggcctcgtc ccccatcgcc gcgacgaggt
     3901 tcgtcgagcc gacgatgtcg gtgaacatga acgtcgggtg gacccgctcg gctacctctt
     3961 cgcggacccc ctcgagcagc ttcgtcaccc gagccgcgtc gagcggcgcg cccagccgct
     4021 cgaacgtcgc gcgcgacgct tccagctcga gcgtggcgga accctcgtcc ccggcggcgc
     4081 gatacgcctc cccgaggagc gcgcgcgtct cggccgcctc gtagggcaag tcgatctcgc
     4141 tccacagccg gcgcgcctgg cgcagcgtcg cgatcgcctc ctcgtgcctc ccctccgcgt
     4201 cctcgacgga accccgggtc gtcagcgcct gcgcgcggaa ggcggtcgtc tcgtagaggt
     4261 ccgcggtcgc gctcagctcc tcgctcatct cgcacgcacg gtccacgtcc ccgccggcga
     4321 gagccacgcg tgcccgcgcc gggaggagct tcgcccgccg cagccgatcc tcggcactca
     4381 gctcctcaag cgcgcggtcg agcgaggtac gtgcctgctc gatcttgcct tcggccagca
     4441 gcagcagggc cagtcccggc tgaggctccc gccccagctc gtgcgcctgc cggaacgcgc
     4501 ttgtcgcacc cgcgacgtcg cccatccgca gtcgcatgtt cccgagctcg tcgaaggcgg
     4561 ctcccgcgtg ggtcagcaac ccgtggctct cgagctccac gcaggctcgc tccacttcct
     4621 cgatcgcgtc cgcccatgac ccgcgcaacc gcatgatctc ggcgcggtgg acgcggcaga
     4681 cgcccgggaa cccgctgatg gactggcgct cgcaccagcg cttcgccgcc tccgtccact
     4741 ccccggcgcg gccgtagtcg cccagcctcg cacacgcgtt gatgacgttg cagtacacga
     4801 cgccggtcac gaacggtgag agctcgcccg agacggcggc caccgtcgcc tcatcctgga
     4861 ggctgcgtcc ctcttccacg cggccgaggg ccacgagcgc gtcgcccttc ccctgcaggg
     4921 cgagcgccag gaggtcgcgg tcaccgaggc gcgtcgccag gtccagcgcc tcctcgcaca
     4981 gccccagggc gcgctcgtgg tcgccgtcct tctctgcggc ggctcgcgcc ctggcccgca
     5041 ggagccaccc gtgcgctcga gactccggca gcccctccaa caggcgctcg gcgcgactga
     5101 gccacgcacc cgcgatcgct ccgtcggcgc ggttgaggtg gtcgcgagcg aggatgagcg
     5161 cgaacatggc cgcctcctcg ggctgactcg catcgaggta cgcggcgtag gcccgttcgc
     5221 gcgccgagat ggcctccccg atgtgcccag cccaccaggc ggcctcaccg agcgccgcga
     5281 tgtcctccgg cgcgagcgaa ccctctcggt ccgcacgcga caggaggtcg tacccctcgc
     5341 tccacgcgtg ccggtcgatc gccgcacgcc ctgctgcgac cgggtcctct tcgagggaag
     5401 gcgcgtccgt cacgctcgtc attctgacac cgcgcccgag gggccgggct acccgcttga
     5461 ggcggcccga tcctgccagg gaagaacggg ggcgtcggcc cagaggcgct cgagcgagta
     5521 gaactcccgc tcctcggcgt ggaagacgtg gacgacgatg tcgacgtagt cgagcaggac
     5581 ccagcggccc tcgcgctccc cctcccgccg gacgggcttc acgtcctggt tccggagcgc
     5641 gcgctggacc tcctcggcga tcgtccggac ctgtcggtcg ttcgtgcccg aggcgaggac
     5701 gaagaaatcc gtgatgccga tcagcgaccg cacatccatc acgacgatgt cctcggcctt
     5761 cttcgaggag gccgaccgcg cggccacgat cgcgaggtcg cgggagctcg tcacgcggcc
     5821 cggaagtccc tcccgacgat caccgtgacc tcggcgaccc gcgacgactg cggatcgagg
     5881 gcgacccgcc cgacgccgag cagctcccgg gcgagcctcg cctcatccag gaaccgctcg
     5941 tcggtggccg cgatcagtgt ctccttcacg tcgaacgtcg atgcgttctg cgaggatacc
     6001 agccggaacc cggccggaac gagcttccgc gcgacccgct cgccgatccc ggggatcccg
     6061 ttgccgttga ggacgaccag ccgcaccggc tcggccgtgc gcgccgtgcg tgcgaaccgc
     6121 tcgcgcgcga gcgccgcgac cgcctcggcc tcggcccgaa cgagcccgga ccccgcctcg
     6181 acgaccggca ggtcgatcgc gcgggccccg ccgaccccgg cagaggtcac gatccgggcc
     6241 gcgtcgcgcg ggagcgcccc ggcgaggacg cgcgggcggg cggcggtcgc agccaggaga
     6301 ccgtccagca c
//
LOCUS       DATLGG010000217         9665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_46220, whole genome shotgun sequence.
ACCESSION   DATLGG010000217 DATLGG010000000
VERSION     DATLGG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46220"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VM638_06155"
     CDS             <1..387
                     /locus_tag="VM638_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVM12039.1"
                     /translation="IPERAPVEDHYALNRAAEELRALGIRLAADDAGAGFESLGHILE
                     LRPELIKFDVALTRDVDTDPVRHGLARSLVDFARQFDALLVAEGIETRAELEALRELG
                     FGFGQGFFLGRPGGLPLAERPLPQDD"
     gene            complement(384..1226)
                     /locus_tag="VM638_06160"
     CDS             complement(384..1226)
                     /locus_tag="VM638_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVM12040.1"
                     /translation="MAERPFPRGRSTVRDPRTVVARVGEHLNGHRDQDRRLRAVEAEV
                     RDVLRGGGPRIVFQPIVDLRNGDVVGLEALSRFTGSHRPPLAWFEAARQVGLGCELEL
                     LALRRARAACAAADGIFLAVNLSPETLVSPAFLELLDGEDHRLVIEATEHAPVHDYDA
                     LARPLRTIKEKGARLAVDDAGAGFASLRHVLRLAPDLIKLDIELTRQIDSDRAKRALA
                     AGLIAFAEELPATIVAEGIETREQLDELRELGVTLGQGFYLCPPSTIGDIDPEAVAER
                     AVDR"
     gene            1399..2289
                     /locus_tag="VM638_06165"
     CDS             1399..2289
                     /locus_tag="VM638_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12041.1"
                     /translation="MNTKALTCVLTLAGVLAAAVPAGAAGAVTVDSATRAGDTLAVGG
                     GSTYAAQPNVTLGTDAREDAPPGFAQIGGDLASVALSTNLDGETTLRLLWAVSPGLNS
                     VPLVTHGWTFCISGDGCYELQTQRFTFGTHNSLPTAADSALYRCADETCEIEGRTKLT
                     TPWIPTYFDGQGSTTTLPAALGLAPGKVITSVTADPLGPVYAAAGFANPATVQRGTVD
                     GIPTVAAYEVPRRQVSVAVAQPGLNPAQVDYGTSVTPNPNGTWSASLDVAGLSGSYTV
                     YARACYGANNCAYTTRSVSL"
     gene            2337..3230
                     /locus_tag="VM638_06170"
     CDS             2337..3230
                     /locus_tag="VM638_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12042.1"
                     /translation="MKRMIGSGLAVGLLALAVPAGASGTVSVTSTTRTGDTLRASGGS
                     TFAAQPYLTVGTDASGDAPDGWQQTGGDLVKAEISGTLEDTLRFRVTVDQMLALVAGF
                     PAYQFGWNFCVSGGSCYELNLTRVKADMANNTAAAPPHWYLFRCGNEQCTPANQIRVA
                     DGQAQVFQSDRTYIATVPMLSMGARPGDVITPKAVDAKGPVFSAFGAPYPTQMQRGSG
                     DGIETVAPYRIPKREVSVAVDAPGQAPDSVGYTTTVTPAATGAWSATFDVFGRSGAHA
                     VYAKACFGENNCAYAQAPVTL"
     gene            complement(3306..4829)
                     /locus_tag="VM638_06175"
     CDS             complement(3306..4829)
                     /locus_tag="VM638_06175"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HVM12043.1"
                     /translation="MTRPMRAKKIHDNPSPEELRAFSEEMENARVTEFGNLNVQTRAT
                     SRSAGSTYIVTDDPSITSGQTITREEYERIAKLQDDYIAEQEMIVVDGYIGNVEGFRT
                     PARLTIEKRNANIAGMQQKLYYPREGGEPEVQIIYTPNLPLPGYPDDRCIAVDLDNGV
                     TRVLNSDYFGESKKGGLRMWNTIVYDRGGLALHAGCKVIPTDRGEKTFLIIGLSGTGK
                     TTTTFTTQNESLPVQDDFVALMPGGKVYGTENGCFAKTFSLDPDFEPNIYNAVTRPES
                     YLENVYQDDEGNVNFFETSYTKNGRAVFGMDALGRFRDPREVGTVDYLLILNWNENII
                     PAVAKLTQAQAAAYFMLGETTGTSAGGKAEEGKFLRVPGTNPFFPLRHDQQGNRILEL
                     LGSHPIEVFLMNTGRVGGKADDERSKKVKIPHSSAVVKAIAEGTIRWTEDPDFGYQVA
                     ESVPDLDDPDLLQPRRLYEAQGRIAEYDAFVDRFKTERVAFLERFEGLEDEIIKAVG"
     gene            complement(4893..5102)
                     /locus_tag="VM638_06180"
     CDS             complement(4893..5102)
                     /locus_tag="VM638_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVM12044.1"
                     /translation="MPVINTIELEGVSEMSWGDAAREALREASKTIRNITRLEVLGTA
                     TEVEGNRIARYRTEVRLYFEVEQSR"
     gene            5167..6168
                     /gene="thiL"
                     /locus_tag="VM638_06185"
     CDS             5167..6168
                     /gene="thiL"
                     /locus_tag="VM638_06185"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HVM12045.1"
                     /translation="MSQLSEDELLRAIRKVVAEDAPGVVIPVGDDAAVVDPGRALSVL
                     TADLLIEGVHFDLDATSAHDLGYKAVAVNVSDLAAMGAAPRFALVSLGLQPDVDAAWV
                     MDLYGGMREAAGEYGLALVGGDLSRAKERVIGVTAVGAVAKGKAVTRSGARPGDAIVV
                     TGALGAAAAGLLLAKRAPHDVRGPLGTDEGKEAVRALLRPHARVGEGETLAQAGATAM
                     IDLSDGLAIDLHRLCEESGTGARVRLADVPLATGLEGLAAALEAEAWPLALGGGEDYE
                     LLATLSPEVVEPTRQKLRDRFGTPLTRIGEITDADVVAVGPDGLEQPLERAGWEHFG"
     gene            6161..6958
                     /gene="thiD"
                     /locus_tag="VM638_06190"
     CDS             6161..6958
                     /gene="thiD"
                     /locus_tag="VM638_06190"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HVM12046.1"
                     /translation="MAEPRRALTVAGSDSGGGAGIQADLKTFQALGVFGTTAITAVTV
                     QNTKGVSGYEEISPEVVAEQIRAVVTDIGVDAAKTGMLASAPIVEAAAEALRETRVPN
                     LVVDPVFLSKHGHPLLQEDAVEALRRRILPLATLVTPNLPEAEGLAGFPIRSDDDMER
                     AGRAILDLGARAVLVKGGHREGPQATDLLVMADRVEWIEGSRIDTPHTHGTGCVLAAA
                     AAAYLARGEDLPEAVRGAKRFVTGAIRHALPIGQGIGPVDPAWRDRA"
     gene            7020..9080
                     /locus_tag="VM638_06195"
     CDS             7020..9080
                     /locus_tag="VM638_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12047.1"
                     /translation="MRSLRAVPVLLCSLVLSLVATTPAAAYQRPGLTVRLSESAQGEQ
                     GNHESTHSAISHDGRFVAFASQSSNLVPGDTNGSLDIFVRDLVGGTIERASVSSTGVQ
                     SDRNSFNRPAISPDGRFVAFQSLGTNLAPGGGNGSAQIFLRDRARGTTERISIASDGA
                     MGNGISSQPAISADGRFVVFQSQASNLVPDDTNATYDVFVRDRVEGTTERVSVSTDGA
                     AANAGMYDSPAISADGRYVAFATQASNLVADDTNAALDVFVRDRAAGTTRRISVSSAG
                     AQGDDHSYAPTMSADGRTVAFISRATTLIPSDANYTYDIFLRDIERGTTERVALTAAG
                     RDPNAESYYPSISADGSVVAFVSEASNLVPGDTNGRSDIFVRNRVTDTTERISASLAG
                     GASNERSGSPSLSGDGRVVSFQSFASNLVPGDTNGRGDVFVRDRGPALGLGGLTARVE
                     NEGVAVSGWSTFSGATISAATDAEDVLPVTGLAGSDIARARVVHRPEDEDLLLRIDLA
                     ALPSRDGWASPWAHFGFRFSVGATRYEVRSISGLTGSSLYRCAPDCIEQERLSGGVGT
                     AGEDVLVAVPLAAVGALPGAQLTDVSAYTALGPPQARLDETMVDELDLPSAVVPRIEV
                     TLGLASAETPEEEVRFDVAAPLEVGRFSRILDGRALGSGEHRVWARACLGGTCSTSFR
                     VVEG"
     gene            9160..>9665
                     /locus_tag="VM638_06200"
     CDS             9160..>9665
                     /locus_tag="VM638_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12048.1"
                     /translation="MTRRASVALLALLLLIALSSPAAAYPRPGRTDQVSVAPSGKAAD
                     GFVVYPSISGDGRHVVFVSDAPNLVAGISPEGDHVFAHDRATRRTEIVSVRSDGTLPP
                     GRSKIATVSDDGRSVAFDSTERLVPEDTGDHLDIYVRDRALDVTRRASVGHDGRQANA
                     DAGLHGLSG"
BASE COUNT         1344 a         3429 c         3447 g         1445 t
ORIGIN      
        1 atcccggagc gcgcgccggt ggaggaccac tacgccctga accgggccgc ggaggagctg
       61 cgggcgctcg gcatccgtct cgccgccgac gacgccggcg ccggcttcga gagcctcggg
      121 cacatcctcg agctccggcc ggagctgatc aagttcgacg tcgccctgac ccgcgacgtc
      181 gataccgatc ccgtccgaca cgggctcgca cggtccctcg tcgacttcgc gagacagttc
      241 gacgcgctct tggtcgccga ggggatcgag acccgtgcgg agctcgaggc gttgcgggag
      301 ctcgggttcg gcttcggtca ggggttcttc ctagggcgcc ccggcggtct gccgctcgcc
      361 gagcggcccc tgccgcagga cgactagcgg tcgacggccc gctccgcgac ggcctctggg
      421 tcgatgtccc cgatcgtcga cggcgggcac aggtagaagc cctgaccgag cgtgacgccg
      481 agctcgcgta gctcgtcgag ctgctccctc gtctcgatcc cctccgcgac gatcgtcgcc
      541 ggcagctcct cggcgaaggc gatcaggccc gccgccagcg cccgcttcgc tcggtcggag
      601 tcgatctgcc gcgtgagctc gatgtcgagc ttgatcagat ccggagccag gcgcaggacg
      661 tgccggaggc tggcgaatcc cgcgccggcg tcatcgaccg ccaggcgggc gcccttctct
      721 ttgatcgtgc ggaggggccg ggcgagggca tcgtagtcgt ggacgggcgc atgctccgtc
      781 gcctcgatca cgagccggtg gtcctcaccg tcgaggagct cgaggaaggc cggcgagacc
      841 agggtctcgg gtgagaggtt caccgcgagg aagatcccat ccgctgctgc acacgccgct
      901 ctcgcgcgac gcagcgcaag aagctcgagc tcgcagccga gcccgacctg ccgtgccgcc
      961 tcgaaccagg cgagcggcgg ccggtgcgat cccgtgaacc gcgagagggc ctcgaggccg
     1021 acgacatcgc cgttgcgcag gtcgacgatc ggctggaaga cgatgcgggg gccgcctccg
     1081 cgaagcacgt cgcggacctc ggcttcgacc gcacgcaagc gccggtcctg gtcgcggtgg
     1141 ccgttgaggt gctcgccgac gcgcgcgacg acggtccgcg ggtcgcggac ggtggaccgg
     1201 ccgcggggga agggacgttc ggccatcgtt cgctcgttat gcgccggttc gggcgtccgc
     1261 gcaagtcccc catccgtccg acacggtcgc gctggcgctg gcggcccctc cgccaccgcg
     1321 ggccggaggg gtggacgtct tccgccccga atcccatcta tgaggcgcgg tgccctgccg
     1381 cgccgggagg aggacgggat gaacaccaag gcactcacgt gcgttctcac gcttgccggc
     1441 gtgctcgcgg cggcggtccc cgccggtgcg gccggcgctg tgacggtcga ctccgcaacg
     1501 cgggcgggag acaccctcgc ggtcggcgga ggctccacct acgcggctca acccaacgtc
     1561 accctcggga cggatgcccg ggaggacgcg ccacccgggt tcgcccagat cggaggggac
     1621 cttgcgtcgg tcgcgttgag cacgaacctc gatggcgaga cgacgctgcg cctgctctgg
     1681 gcggtgtcac cgggactgaa cagtgtcccg ctggtgacgc acgggtggac gttctgcatc
     1741 tcgggggacg gctgctacga gctccagacg cagcggttca ccttcggcac ccacaacagc
     1801 ctgccgaccg cggctgacag cgccctgtac cgctgcgccg acgagacctg cgagatcgag
     1861 gggcggacca agctcacgac accgtggatc ccgacctact tcgacggcca gggctccacg
     1921 accacgctgc cggcggcgct cggcctcgcg ccggggaagg tcatcacctc cgtcaccgcg
     1981 gaccctctcg ggcccgtcta cgccgcggct ggcttcgcca accctgcgac cgtccagcgc
     2041 ggcaccgttg acggcatccc gacggtcgcg gcgtacgagg tcccccgccg ccaggtctcc
     2101 gtcgcggttg ctcagccggg actcaacccc gcccaggtcg actacggcac cagcgtcacc
     2161 ccgaacccga acggcacctg gtcggcgtcg ctcgacgtgg cgggtctcag cggcagctac
     2221 acggtctacg cccgcgcctg ctacggcgcg aacaactgcg cctacacgac gaggtccgtc
     2281 tcgctctgag gcggttcgag agcggcatgc gtcgcgaaga ggaaaaggag aacgggatga
     2341 agaggatgat cggatcggga ctcgcggtgg ggctcctggc gctcgccgtg ccggccgggg
     2401 cgtcggggac cgtcagcgtc acgtccacga cgaggaccgg ggacacgctg cgcgcatctg
     2461 ggggatccac cttcgcggcc cagccgtacc tgaccgtcgg gacggacgcc tcgggtgacg
     2521 ctcccgatgg atggcagcag accggcggcg atctggtgaa ggcggagatc tccggcacgc
     2581 tggaggacac cctgcggttc cgagtgaccg tcgatcagat gctcgcgctc gtggcggggt
     2641 tcccggcgta ccagttcggc tggaacttct gcgtcagcgg cgggagctgc tacgagctca
     2701 acctgacgcg cgtgaaggcg gacatggcga acaacaccgc tgctgcgccg cctcactggt
     2761 acctgttccg gtgcggcaac gagcagtgca cgccggcgaa ccagatccgg gttgccgatg
     2821 ggcaggcgca ggtcttccag agcgaccgta cctacatcgc gaccgtgccg atgctttcga
     2881 tgggtgcgag gccgggcgac gtgatcacgc cgaaggcggt ggacgcgaag gggcccgtct
     2941 tcagcgcctt cggggcgccc tacccgacgc agatgcagcg ggggtccggt gacgggatcg
     3001 agaccgtggc cccgtaccgg atcccgaagc gggaggtgtc ggtcgcggtc gacgcgccgg
     3061 gacaggcgcc cgactcggtc ggctacacga ccacggtcac gcccgcggcg accggggcat
     3121 ggtcggccac cttcgacgtg ttcggtcgga gtggcgccca cgcggtctac gcgaaggcgt
     3181 gcttcggcga gaacaactgc gcgtacgccc aggcgccggt gacgctgtag gaccccgaac
     3241 gttcgaagcc cccggctgcc cgtggccggg ggcttcgttc gttccgggtg accccggctg
     3301 agcggctagc cgaccgcctt gatgatctcg tcctcgaggc cctcgaagcg ctcgaggaac
     3361 gcgacccgct ccgtcttgaa ccggtcgacg aacgcgtcgt actccgcgat ccgcccctgc
     3421 gcctcgtaca gccggcgcgg ctggagcagg tcggggtcgt cgaggtccgg gaccgactcc
     3481 gcgacctggt agccgaagtc ggggtcctcg gtccagcgga tcgtcccctc ggcgatcgcc
     3541 ttcacgaccg cggacgagtg cgggatcttg accttcttcg agcgctcgtc gtcggccttg
     3601 ccgccgacgc gcccggtgtt catgaggaac acctcgatcg ggtgcgaccc caggagctcg
     3661 aggatccggt tcccctgctg gtcgtgccgg agcgggaaga acgggttcgt cccggggacc
     3721 cggaggaact tgccctcctc ggccttcccg cccgcggagg tgccggtcgt ctcgccgagc
     3781 atgaagtacg cagcggcctg cgcctgcgtc agcttcgcga cggccgggat gatgttctcg
     3841 ttccagttca ggatcagcag gtagtcgacg gtcccgacct cgcgcgggtc gcggaagcgc
     3901 ccgagcgcgt ccatcccgaa caccgcgcgg ccgttcttcg tgtacgaggt ctcgaagaag
     3961 ttcacgttgc cctcgtcgtc ctggtacacg ttctcgaggt acgactcggg tcgggtgacg
     4021 gcgttgtaga tgttcggctc gaagtcgggg tcgagcgaga acgtcttcgc gaagcagccg
     4081 ttctcggtcc cgtagacctt gccgcccggc atcagcgcga cgaagtcgtc ctgcaccggc
     4141 agtgactcgt tctgggtcgt gaacgtcgtg gtcgtcttgc cagtgccgga gaggccgatg
     4201 atgaggaagg tcttctcgcc gcggtcggtc gggatcacct tgcatcccgc gtggagcgcg
     4261 aggccgccgc ggtcgtagac gatcgtgttc cacatgcgga ggccgccctt cttcgactcc
     4321 ccgaagtagt cggagttgag gacgcgcgtg acgccgttgt cgaggtcgac cgcgatgcag
     4381 cggtcgtcgg ggtagccggg cagcggcagg ttcggcgtgt agatgatctg gacctcgggc
     4441 tcgccaccct cgcgcgggta gtagagcttc tgctgcatcc cggcgatgtt cgcgttgcgc
     4501 ttctcgatcg tgaggcgcgc gggcgtgcgg aagccttcca cgttgccgat gtagccgtcg
     4561 acgacgatca tctcctgctc ggcgatgtag tcgtcctgga gcttcgcgat ccgctcgtac
     4621 tcctcgcgcg tgatcgtctg cccgctcgtg atcgacgggt cgtccgtcac gatgtaggtg
     4681 ctccccgcgc tgcgcgaggt ggcccgggtc tggacgttca ggttcccgaa ctccgtgacc
     4741 cgcgcgttct ccatctcctc gctgaacgcg cgcagctctt cgggggaggg gttgtcgtgg
     4801 atcttcttcg ctcgcatcgg tcgcgtcacc tcgtggtcgg gaaagacgct cagtctactc
     4861 cagcgaggcg ggcatcaacc gcgaagcggg cgtcagcggc tctgctcgac ctcgaagtag
     4921 aggcgcacct cggtgcggta gcgagcgatg cggttgccct cgacctcggt ggcggtgccg
     4981 aggacctcga gccgcgtgat gttgcggatc gtcttcgacg cctcccgcag ggcctcgcgg
     5041 gcggcgtccc cccacgacat ctccgagacc ccctccagct cgatcgtgtt gatcaccggc
     5101 atcgcatcct cctcggccgt cgtatcccgg tcgattatgg tgaagcggtc ggccgaaggg
     5161 aacccgatgt cccagctctc cgaagacgag ctcctccgcg cgatccggaa ggtcgtggcc
     5221 gaggacgcgc cgggcgtggt gatcccggtc ggcgacgacg ccgcggtcgt ggaccccgga
     5281 cgggcgctct ccgtgctgac ggcggacctc ctgatcgagg gtgtccactt cgacctcgac
     5341 gcgacctccg cccacgacct cggctacaag gcagtcgccg tgaacgtgtc cgacctcgcg
     5401 gcgatgggcg cggccccgcg cttcgccctc gtctccctcg gcctgcagcc cgacgtggac
     5461 gcagcctggg tcatggacct ctacggaggc atgcgggagg cggccggcga gtacggcctg
     5521 gcgctggtcg gcggcgacct gagccgcgcg aaggagcgcg tgatcggggt gacggcggtc
     5581 ggagccgtcg cgaaggggaa ggcggtgacg cgctcggggg cgcgccccgg cgacgcgatc
     5641 gtcgtgaccg gagcgctcgg ggccgcggcg gcgggcctcc tgctggcgaa gcgcgctccc
     5701 cacgacgtgc gcggaccgct gggcaccgat gaggggaagg aggcggtccg ggccctgctc
     5761 cgtccgcacg cccgcgtcgg ggagggggag acgctcgcgc aggccggcgc caccgcgatg
     5821 atcgatctct ccgacggcct cgcgatcgac ctgcaccgcc tgtgcgagga gagcgggacg
     5881 ggcgcccgcg tccgcctcgc cgacgtgccg ctggcgacgg ggctcgaggg tctcgcggcc
     5941 gcgctcgagg ccgaggcctg gcccctggcg ctcggcggcg gcgaggacta cgagctgctg
     6001 gcgacgctct cgccggaggt cgtggagccc acgcggcaga agctgcggga tcgcttcggc
     6061 acgccgctca cgcgcatcgg cgagatcacc gacgccgacg tcgtcgccgt cgggccggac
     6121 ggcctcgaac agccgctcga gcgcgcgggg tgggagcact ttggctgagc cgcggcgcgc
     6181 gctcacggtt gcgggttcgg actcgggcgg cggggcgggg atccaggccg acctcaagac
     6241 cttccaggcg ctcggggtct tcggcacgac ggcgatcacc gccgtcaccg tgcagaacac
     6301 gaagggcgtg tccggctacg aggagatctc gccggaggtc gtggcggagc agatccgcgc
     6361 ggtcgtgacg gacatcgggg tcgacgcggc gaagaccggg atgctcgcct ccgccccgat
     6421 cgtcgaggcg gcggcggagg cgctgcgcga gacgcgggtg cccaacctcg tggtcgaccc
     6481 cgtgttcctc tcgaagcacg ggcacccgct cctccaggag gacgcggtgg aggccctccg
     6541 tcggcgcatc ctgccgctgg cgacgctcgt gacgccgaac ctgcccgagg ccgaaggtct
     6601 cgcgggtttc ccgatccgct cggacgacga catggagcgg gccgggcgcg cgatcctcga
     6661 cctcggcgcg cgagcggtcc tcgtgaaggg cggccaccgt gagggtccgc aggcgacgga
     6721 cctgctggtg atggccgacc gcgtggagtg gatcgaggga agccgcatcg ataccccgca
     6781 cacgcacggg accggctgcg tcctcgcggc tgccgccgcc gcgtacctcg cgagggggga
     6841 ggacctcccc gaagcggtcc gcggggcgaa gcgcttcgtc acgggggcga tccgccacgc
     6901 cctccccatc gggcagggga tcggcccggt cgacccggcc tggcgcgacc gggcctgagg
     6961 cccgctctgc tgtgcagctg cacaaagggt cgtcgccgat ccgcgtcgaa cacagcggca
     7021 tgcgatccct ccgggccgtc ccggtcctgc tgtgctcgct cgtcctgtcg ctggtggcga
     7081 cgactccggc cgccgcctac cagcgtcccg gcctgaccgt gcgcctctcc gagagcgccc
     7141 agggagaaca ggggaaccac gagtcgaccc actcggcgat cagccacgat ggccgcttcg
     7201 tggcgttcgc gagccagtcg tcgaacctcg tgccgggcga cacgaacgga tcgctcgaca
     7261 tcttcgtccg cgacctcgtc ggcgggacga tcgagcgggc ctcggtctcg agcaccggcg
     7321 tccagagcga ccggaactcg ttcaaccgtc ccgcgatctc tcccgacggg cgcttcgtcg
     7381 cgttccagag cctcgggacg aacctcgccc ccggcggagg gaacgggtcg gcgcagatct
     7441 tcctgcgcga ccgggcgcgc ggaacgaccg agcggatctc gatcgcgagc gacggcgcga
     7501 tggggaacgg gatctcgtcg cagccggcca tctcggccga cggacggttc gtcgtgttcc
     7561 agagccaagc ctcgaacctc gtgccggacg acacgaacgc cacgtacgac gtgttcgtcc
     7621 gcgaccgcgt ggagggaacc accgagcgcg tgagcgtgtc gaccgacggg gccgccgcca
     7681 acgcgggcat gtacgactcg ccggcgatct ccgccgacgg ccgctacgtg gccttcgcga
     7741 cgcaggcctc gaacctcgtg gctgacgaca cgaacgccgc gctcgacgtc ttcgtccgcg
     7801 accgcgcagc cgggaccacg cgacggatct ccgtgtccag cgcgggcgcc cagggcgacg
     7861 atcactccta cgccccgacg atgagcgcgg atgggcggac cgtcgcgttc atcagccggg
     7921 cgaccaccct gatcccgagc gacgccaact acacctacga catcttcctg cgcgacatcg
     7981 agcgcgggac gacggaacgc gtggcgctca cggcggcggg tcgcgatccg aacgccgagt
     8041 cctactaccc atcgatcagc gccgacggct cggtcgtcgc gttcgtgagc gaagcgtcca
     8101 acctcgtccc cggcgacacg aacggccgct cggacatctt cgttcggaac cgggtcaccg
     8161 atacgaccga gcggatctcc gcctcgctcg cgggcggcgc gtcgaacgaa cgatcgggtt
     8221 ctccgtcgct gagcggcgac ggccgcgtcg tctcgttcca gagcttcgcg tcgaacctgg
     8281 tgcccgggga cacgaacggc cgcggcgacg tgttcgtgcg cgaccgtggc cccgccctcg
     8341 gcctcggcgg cctgacggct cgggtcgaga acgagggggt cgccgtctcg gggtggtcga
     8401 ccttctcggg cgcaacgatc tccgcggcga ccgacgcgga agacgtcctc cccgtgaccg
     8461 gcctcgctgg atccgacatc gcccgggcac gcgtggtgca tcggcccgag gacgaggatc
     8521 tgctcctccg gatcgacctg gccgcactcc cgagccgcga cggctgggcg tccccctggg
     8581 cgcatttcgg gttccggttc tccgtgggag cgacccgcta cgaggtccga tcgatctcgg
     8641 ggctcaccgg ctcgagcctg taccgctgcg cccctgactg catcgagcag gagcgcctct
     8701 ccggcggcgt cggaaccgct ggggaagacg tcctcgtcgc ggtcccgctc gcggccgtcg
     8761 gggcgctccc gggagcccag ctcaccgatg tctccgccta tacggcgctc ggcccgcccc
     8821 aggctcggct cgacgagacg atggtcgacg agctcgatct cccgagcgcc gtcgttccgc
     8881 gcatcgaggt gacgctcggg ctcgcctccg ccgagacccc cgaggaggag gttcggttcg
     8941 acgtcgccgc cccactggag gtcggtcgct tctcacggat cctcgacggg agggcgctcg
     9001 gctccgggga gcaccgcgtg tgggcacgtg cgtgcctcgg gggcacctgc tcgacgagct
     9061 tccgggtcgt cgagggctga cgtctcgccg cgcgcgggcg gcagctttgg gcagttgcac
     9121 agaggaaggc gagcatcccg gggcgaatct ccggggatga tgaccagacg cgcctccgtc
     9181 gcgctcctcg cgctgctgct cctcatcgcg ctctcgtcgc cggcggccgc ctacccgcgt
     9241 cccggccgga ccgaccaggt ctccgtcgcc ccgtccggga aggcggcgga cggcttcgtc
     9301 gtctacccct cgatctccgg cgacggacgt cacgtcgtgt tcgtgagcga cgcaccgaac
     9361 ctcgtcgccg gcatctcccc ggagggggac cacgtgttcg cgcacgaccg cgcgacccgt
     9421 cgcaccgaga tcgtctcggt ccgctccgac ggcacgctgc cgccgggacg gagcaagatc
     9481 gcgacggtca gcgacgacgg ccgatcggtc gcgttcgact cgaccgagcg cctcgtgccg
     9541 gaggacaccg gggaccacct cgacatctac gtgcgcgacc gcgccctcga cgtgacgcgg
     9601 cgcgcgtcgg tcggccatga cggacgacag gcgaacgccg acgccgggtt gcacggcctc
     9661 tccgg
//
LOCUS       DATLGG010000218         7152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_139488, whole genome shotgun sequence.
ACCESSION   DATLGG010000218 DATLGG010000000
VERSION     DATLGG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139488"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VM638_06205"
     CDS             complement(<1..861)
                     /locus_tag="VM638_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM12049.1"
                     /translation="MVAVPEDFDRGFEEAYRAHYPALRRRSRRWLPADEVDDAVQETF
                     LRAWAHHGGRDPGMGWLVTVLRNLAIDRARRRTAEPVGDIEKLDSPTDEGPDDKVVAL
                     EERRAVRQAMLDLTESQRTVLHLREWDGKSHQEIADSLGTTIPSVESLLVRGRRRLRG
                     ALEKVMGVVLWPASGAWRKLRGLPEAGAGAAVNPVAVGAVSTAVAHFATAAAVIVAGV
                     AGGGGSGRGEEREPIRPSEGYTAAVAGGSEQVGSRRGASAGFTRETSSTKETARGSTS
                     GGGSTASTETS"
     gene            1024..1410
                     /locus_tag="VM638_06210"
     CDS             1024..1410
                     /locus_tag="VM638_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM12050.1"
                     /translation="MEDNGASTKILVADDDPVILRLLQVNFGLEGFEVHTASRGEEAL
                     ERAREMHPDIILLDVMMPGLDGWAVCQRLKEDERFTDTPIVFLSARAQDEDRKRGYGL
                     GVAAYVTKPFDPGRLVELVRRLISAD"
     gene            1427..3115
                     /gene="argS"
                     /locus_tag="VM638_06215"
     CDS             1427..3115
                     /gene="argS"
                     /locus_tag="VM638_06215"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HVM12051.1"
                     /translation="MIEAELVRLIRTAVEATAAELALDGPAPEIEILRPRQKEHGDYA
                     TGVALALAAGAGGRNPRAIAEALLRHLPPADFVDRAEVAGPGFINFFVTHGWLHGVVE
                     QVRRLGGDYGRVDVGAGERVLVEFVSANPTGPLHVGTGRNAAVGDALARVLAFAGYEV
                     SREYYVNDAGSQMELFGASVEARYLEHFGRPAEVPERGYHGAYVRELAAAIAQDLGDE
                     PLDMPAPERRRKLAEEGMARALEGIRGTLERFGVRFDTWFRESTLHETGRVQQALELL
                     RERDLAYEAEGALWFRAERFGDDKDRVLVRASGDPTYFAADTAYLRDKFARGFDHLIY
                     VWGADHHGTVKRLEGAATAFGYEVDRVEFILTQLVSLYRDAEPVRMSKRTGDIVTLDE
                     LVEEVGPDAARYTLLTRSVDSALDFDIEHVKRRSLDNPVYYVQYAHARIASVIRFARE
                     QGVELGEEEAPADLLTHETELDLMRKIAELPSLIRLVADGRAPHRLTRYAEEVAAGFH
                     RFYTACRVVTEDADLTRARLHLAAATRQVIANVLGLLGVSAPESMERLGEEDEA"
     gene            3112..4908
                     /gene="lysA"
                     /locus_tag="VM638_06220"
     CDS             3112..4908
                     /gene="lysA"
                     /locus_tag="VM638_06220"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVM12052.1"
                     /translation="MSHPWPEGVRFGDEGLTFRGRTARDLVARYGTPLVLVDETVVRE
                     RCRSIAEAFGRALWAVKAFPAGALIRIAHEEGLGLLAATGGEVDACLRAGVPASRVVL
                     HGNNKTDAELRLAVGAGVGLLILDNEEEVGRVADVGRAHGVRQPVLVRVVPGLEVDTH
                     EAVATGVVDTKFGVPMPLALRVLRLALEAPELDLRGIHLHLGSQLLDASPHEAAIDVA
                     LDLLVEARRELGWTAETLDLGGGMGVAYTDERALDLRGLAATVRSRVVDGAAARGLEP
                     PEVVVEPGRAVTAPAALTLYTVGAIKEVPGVRTYVAVDGGMSDNLRPALYGSRYTMAL
                     ASRRSDAEARPATVVGRHCESGDILARDVPLPSDLRRDDLLAVAGTGAYEYAMSSNYN
                     KVGRPAVVLATETTDRAILRREDAGDLARLELPTAPRPAAPRPAGVEVRPARVRDARS
                     LARMVESVAAEGRFIRTEGPITVRETRRRLRVGWSPDHAELVAVADGAVVGHLGIGRT
                     PHAGTRHVASLGMAVAAPWRGRGVGSALLGAAFDWARWAGVEKLSLEVYPHNTVAMAL
                     YRKFGFVEEGRLRAHSKKRGTYEDEIVMGAWV"
     gene            4899..6191
                     /locus_tag="VM638_06225"
     CDS             4899..6191
                     /locus_tag="VM638_06225"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVM12053.1"
                     /translation="MGVTRTLGVGMLGCGTVGASVARLLHDGAPSIARRTGATLEVRR
                     VGVRDPGRDRSVPLPPDAFTADLRSVVADPSVDVVLELLGGIEPARALIRAAFDAGKH
                     VVTANKELVATHGRELLEAAAAAGVALRFEAAVGGGIPLVHPLRESLAGDRITRVIGI
                     VNGTTNYVLTRMTEEGLSFDDALTEAQALGFAERDPTDDVEGHDAAAKCAILAALAFD
                     VAIAAPDVYREGITGVRPEDIEFAARLDHVVKLLAVAELADGEVAARVHPAMLPREHP
                     LASVRDSFNAVFVEGEHAGQLMFFGRGAGGAPTATAVVGDLIDVARDIARGARGPAVV
                     PVADRPIRPIEAMQGQYYVCLHVADRPGVLAQVAGAFGSNDVSIKSVWQEGTGEEALL
                     VLITHRANEGDLRRTMDDLRGLDAVTEVRSVMRVEGGE"
     gene            6191..>7152
                     /gene="thrC"
                     /locus_tag="VM638_06230"
     CDS             6191..>7152
                     /gene="thrC"
                     /locus_tag="VM638_06230"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVM12054.1"
                     /translation="MARPWRGVIEEYRDRLPVSERTPIVSLQEGGTPLVRSTRLSEET
                     GCHVFLKYEGTNPTGSFKDRGMTLAVSKALEEGATAVVCASTGNTSASAAAYAARAGI
                     PCAVLVPRGGVAMGKVAQTLAAGARVLQVAEGGFDAALRITRSLAERHPIALVNSVNP
                     YRLEGQKTLAFEVADALARAPDLHLVPVGNAGNITSHWMGYRQLREAAEVAALPRMHG
                     FQAEGAAPIVLGHPVEDPTTVASAIRIGDPASWRGAVEAAEASGGSIDAVSDAEILAA
                     YRELAAEGVFCEPASAASVAGLRLLHRRGGLERGLVVVCVLTGHG"
BASE COUNT          940 a         2536 c         2699 g          977 t
ORIGIN      
        1 cgaggtctcg gtgctggccg tcgagccccc gccggaggtc gacccgcgcg cggtttcctt
       61 cgtcgaggag gtctcgcggg tgaagccggc gctggcaccc cgccgggagc cgacctgctc
      121 gcttccccct gcgacggcag cggtgtagcc ctcgctcggt cggatcggct cgcgctcctc
      181 gccgcggccg ctgccgcccc cgcccgcgac gcccgccacg atgacggcgg ccgccgtcgc
      241 gaagtgggcg acggcggtgg acaccgctcc gacggccacc gggttgacgg cggcgccggc
      301 accggcctcg ggcagacccc ggagcttccg ccacgcgccg ctggcgggcc agagcacgac
      361 ccccatgacc ttctcgagcg ctccgcggag gcgtcggcgc ccccgcacga gcagggactc
      421 gaccgacggg atcgtcgtgc cgagggaatc cgcgatctcc tggtgcgact tgccgtccca
      481 ctcccggagg tggaggaccg tccgctggct ctcggtcagg tcgagcatcg cctggcggac
      541 cgcgcgccgt tcctcgaggg cgacgacctt gtcgtccggc ccctcgtccg tcggcgagtc
      601 gagcttctcg atgtcgccga ccggctcggc cgtgcgacgg cgagcgcggt cgatcgcgag
      661 gttgcgcagg accgtgacga gccagcccat gccgggatcg cggccgccgt ggtgcgccca
      721 cgcgcggagg aacgtctcct ggacggcgtc gtccacctcg tcggcgggga gccagcggcg
      781 ggagcggcgg cgcagcgccg ggtagtgcgc ccggtaggcc tcttcgaagc cccggtcgaa
      841 gtcttcgggg accgctacca tctcaacccc ccgcacaccg gcggtgtcct cctgggagga
      901 ccttgtcctc tcatattcgc cggaggaagg gcgattcctg ccgagccggg agaaatcgga
      961 cggatttttt tcgcttgctc cgctgtcaca ctggcggggc tgatattgct accatccgcg
     1021 gcgttggagg acaacggagc gtccacgaag atcctcgtcg ccgacgacga cccggtgatc
     1081 cttcggctgc tccaggtgaa cttcggtctc gaggggttcg aggtccacac cgccagccgc
     1141 ggcgaggagg cgctggagcg ggcccgcgag atgcacccgg atatcatcct cctcgacgtg
     1201 atgatgcccg gcctcgacgg ctgggcggtc tgccagcggc tgaaggagga cgagcggttc
     1261 accgacaccc ccatcgtctt cctctcggca cgtgcccagg acgaggatcg caagcgggga
     1321 tacggcctgg gcgtcgccgc gtacgtcacg aagccgttcg accccggacg gctcgtcgag
     1381 ctcgtccggc gtctgatctc cgccgactag gccccggacc gcgcgcatga tcgaggccga
     1441 gctcgtccgg ctgatccgga ccgccgtcga ggcaaccgcc gcggagctgg ccctcgacgg
     1501 gcccgcgccg gagatcgaga tcctgcgtcc gcgtcagaag gagcacgggg actacgccac
     1561 gggggtggcg ctcgccctgg ccgcaggtgc cggcggacgg aacccgcgcg cgatcgccga
     1621 ggccctcctg cgacacctcc cgcccgccga cttcgtggac cgcgccgagg tcgcgggccc
     1681 ggggttcatc aacttcttcg tcacccatgg gtggctgcac ggcgtcgtcg agcaggtgcg
     1741 ccggctgggc ggggactacg gccgggtcga tgtcggcgcc ggcgagcggg tgctcgtcga
     1801 gttcgtctcg gccaacccga cgggaccgct tcacgtgggg accgggcgca acgccgccgt
     1861 cggggatgcc ctcgcccgcg tcctcgcctt cgcggggtac gaggtgagcc gggagtacta
     1921 cgtgaacgac gccgggagcc agatggagct cttcggggcg tccgtcgaag cccgctacct
     1981 cgagcacttc ggccgtcccg ccgaggtccc cgagcgcggc taccacggcg cctacgtccg
     2041 cgagctggcc gcggcgatcg cgcaggatct cggcgatgag cccctcgaca tgcccgcgcc
     2101 ggagcggcga cggaagctcg cggaggaagg catggcccgc gcgctcgagg ggatccgggg
     2161 gacgctcgag cggttcgggg tgcgcttcga cacctggttc cgcgagtcca ccctccacga
     2221 gacgggaagg gtccagcagg cgctcgagct cctgcgggag cgggacctcg cctacgaggc
     2281 cgagggggcg ctgtggttcc gagcggagcg gttcggagac gacaaggacc gcgtcctggt
     2341 ccgcgcctcc ggcgacccga cctacttcgc cgccgacacc gcttacctgc gcgacaagtt
     2401 cgcgcgcggg ttcgaccacc tgatctacgt ctggggggcc gatcatcacg ggacggtgaa
     2461 gcggctcgag ggcgccgcga cggccttcgg ctacgaggtg gaccgggtcg agttcatcct
     2521 cacccagctc gtctcgctgt accgggacgc cgagccggtt cggatgagca agcggaccgg
     2581 tgacatcgtc accctcgacg agctcgtcga ggaagtcggg ccggacgccg cgcgatacac
     2641 gctcctgacg cgatcggtgg actccgccct cgacttcgac atcgagcacg tgaagcgccg
     2701 cagcctcgac aatcccgtct actacgtcca gtacgcgcac gcgcgcatcg cgagcgtgat
     2761 ccgcttcgcg cgcgagcagg gcgtggagct cggcgaggag gaggcgccgg ccgacctgct
     2821 gacgcacgag acggagctcg acctgatgcg gaagatcgcg gagcttccgt cgctgatccg
     2881 gctcgtcgcg gacggacgcg cgccgcaccg gctgacgcgg tacgccgagg aggtcgccgc
     2941 cgggttccac cgcttctaca ccgcgtgccg cgtggtgacg gaggatgccg acctcacgcg
     3001 ggcgcgcctg cacctcgccg cggcgacccg gcaggtgatc gcgaacgtgc tcggcctgct
     3061 cggcgtctcg gcgccggaga gcatggagcg cctcggcgag gaggacgagg cgtgagccat
     3121 ccgtggcccg agggggtccg cttcggtgat gaggggctga cgttccgcgg gcggaccgct
     3181 cgcgacctcg tcgcccggta cgggacgccg ctcgtgctgg tcgacgagac ggtcgtccgc
     3241 gagcgctgcc gctcgatcgc cgaggccttc gggcgggcgc tgtgggcggt gaaggcgttc
     3301 cccgccgggg cgctgatccg catcgcgcac gaggagggac tcggtctcct ggcggcgacc
     3361 gggggggagg tcgatgcctg tctccgggcc ggggttccgg cctcacgcgt cgtgctgcac
     3421 ggcaacaaca agaccgacgc cgagctccgg ctggcggtcg gggcgggggt cgggctcctg
     3481 atcctcgaca acgaggagga ggtgggacgg gtcgccgacg tgggccgcgc ccacggggtg
     3541 cgccagcccg tcctggtgcg ggtcgtcccc gggctcgagg tcgataccca cgaggcggtc
     3601 gcgaccggcg tcgtggacac gaagttcggg gtcccgatgc cgctcgcgct ccgcgtcctc
     3661 cgtctggcgc tcgaggcgcc ggagctggac ctgcgcggga tccacctgca cctcgggtcg
     3721 cagctcctcg acgcatcccc gcacgaagcc gcgatcgacg tcgcgctcga cctgctcgtc
     3781 gaagcgaggc gcgagctcgg atggaccgcc gagacgctcg acctcggcgg ggggatgggg
     3841 gtcgcctaca ccgacgagcg ggccctcgac ctgcgagggc tggcggcgac cgttcggtcg
     3901 cgggtcgtcg acggcgcggc ggcgcgaggg ctggagccgc cggaggtcgt cgtggagccg
     3961 gggcgcgcgg tgacggcacc ggcggcgctg accctctaca ccgtcggcgc gatcaaggag
     4021 gtccccggcg tgcggaccta cgtcgcggtg gacggcggga tgtccgacaa cctaaggccc
     4081 gcgctctacg ggtcgcgcta cacgatggcg ctcgcatcgc gtcggtcgga tgcggaggct
     4141 cggccggcga ccgtcgtcgg gcgccactgc gagtccggcg acatcctcgc ccgcgacgtg
     4201 ccgctcccgt cggacctgcg ccgggacgac ctcctcgccg tcgccgggac gggcgcgtac
     4261 gagtacgcga tgagcagcaa ctacaacaag gttgggcgcc ccgcggtcgt gctcgcgacg
     4321 gagacgacgg accgcgcgat ccttcgccga gaggacgcgg gcgatctcgc gcggctcgag
     4381 ctgccgacgg caccgcggcc ggccgcgccg cgcccggcgg gcgtggaggt ccgtcccgcg
     4441 cgtgtgcgcg acgcgcgcag cctcgcgcgg atggtggagt cggtcgctgc ggagggtcgc
     4501 ttcatccgca ccgaagggcc gatcacggtc cgggagacgc ggcggcgcct gcgtgtcggc
     4561 tggagccccg atcatgccga gctcgtcgcc gtggccgacg gtgcggtggt gggacacctc
     4621 gggatcgggc ggaccccgca tgccggcacg agacacgtcg cgtcgctggg catggccgtc
     4681 gcggccccgt ggcgcgggcg cggcgtcggc tcggcgctcc tcggcgcggc gttcgactgg
     4741 gcacgatggg ccggggtcga gaagctctcg ctcgaggtct acccgcacaa caccgtcgcg
     4801 atggcgctct accggaagtt cggtttcgtc gaggaggggc ggttgcgcgc gcattcgaag
     4861 aagcgcggca cgtacgaaga cgagatcgtg atgggcgcat gggtgtgacc cgaacgctgg
     4921 gggtcggcat gctcggctgc ggcacggtcg gtgcatcggt cgcgcgtctc ctgcacgacg
     4981 gcgcgccctc gatcgcgcgg cgcaccgggg cgacgctcga ggtccgacgc gtcggcgtcc
     5041 gcgaccctgg acgggaccga tcggtcccgc ttccacccga cgcgttcacc gccgacctcc
     5101 gctccgtcgt cgccgacccg agcgtggatg tcgtcctcga gctcctcggc gggatcgagc
     5161 cggcgcgcgc cctgatccgc gcggcgttcg acgccgggaa gcacgtcgtg acggcgaaca
     5221 aggagctcgt ggcgacgcac ggccgggagc tcctggaggc cgcggccgcc gcgggggtcg
     5281 cgctccgctt cgaggccgcg gtcggcggcg ggatcccgct cgtgcatccg ctccgtgagt
     5341 cgctcgcggg tgaccggatc acccgcgtca tcggcatcgt gaacgggacg acgaactacg
     5401 tgctcacgcg gatgaccgag gaggggctct ccttcgacga tgccctcacg gaggcgcagg
     5461 cgctcgggtt cgccgaacgc gacccgacgg acgacgtcga gggacacgat gccgccgcga
     5521 agtgcgcgat cctcgccgca ctcgccttcg acgtcgcgat cgcggccccc gacgtctacc
     5581 gcgaggggat cacgggcgtc cgaccggagg acatcgagtt cgcggcgcgg ctcgaccacg
     5641 tggtcaagct gctcgcggtg gccgagctgg cggacgggga ggtcgccgca cgcgtccacc
     5701 cggcgatgct cccgcgcgag caccccctgg cctcggtgcg cgacagcttc aacgcggtgt
     5761 tcgtcgaggg ggagcacgcg ggccagctca tgttcttcgg acgtggcgcc ggcggagcac
     5821 cgacggcgac ggcggtcgtg ggggacctga tcgacgtcgc gcgggacatc gcccggggcg
     5881 cgcgcgggcc cgccgtcgtc cccgtggcgg accgtccgat ccgaccgatc gaggcgatgc
     5941 aggggcagta ctacgtctgc ctacacgtcg ccgaccggcc gggagtcctc gcgcaggtgg
     6001 ccggcgcgtt cggctcgaac gacgtgtcga tcaagagcgt ctggcaggag ggcacggggg
     6061 aggaggccct gctcgtgctc atcacgcacc gcgcgaacga gggtgacctc cgccgcacga
     6121 tggacgacct gcgcggcctc gacgccgtga ccgaggttcg gagcgtcatg cgcgtggaag
     6181 gaggggagta gtggcgcggc cgtggcgcgg ggtgatcgag gagtatcggg atcggctgcc
     6241 cgtgagcgag cggaccccga tcgtgagcct ccaggagggc ggcacgcccc tcgtgcgctc
     6301 cacgcgcctg tccgaggaga ccggctgcca cgtcttcctc aagtacgagg ggacgaaccc
     6361 gaccggctcg ttcaaggatc gggggatgac cctcgccgtg tcgaaggcgc tcgaggaggg
     6421 ggcgaccgcc gtcgtctgcg catcgaccgg caacacgagc gcgtccgcgg cggcgtacgc
     6481 ggcgcgcgcc gggatcccct gcgcggtgct cgtcccccgc ggcggcgtcg cgatggggaa
     6541 ggtcgcccag acgctcgccg ccggcgcccg tgtgctccag gtggccgagg gggggttcga
     6601 cgcggcgctg cggatcacgc ggtcgctcgc cgagcggcac cccatcgccc tcgtcaattc
     6661 ggtgaacccc taccggctcg aggggcagaa gacgctcgcc ttcgaggtcg ccgacgccct
     6721 ggcacgcgcg cccgacctcc acctcgtgcc ggtggggaac gccggcaaca tcacgagcca
     6781 ctggatgggc taccgccagc tccgcgaggc agccgaggtc gcggcgctcc cgcggatgca
     6841 cgggttccag gccgaggggg cggcaccgat cgtgctcggg catccggtcg aggacccgac
     6901 gaccgtcgcg agcgcgatcc ggatcggtga ccccgcctcc tggcgcggcg ccgtcgaggc
     6961 ggccgaggcc tccggcggat cgatcgacgc cgtcagtgac gcggagatcc tcgcggcgta
     7021 ccgggagctc gccgcggagg gcgtcttctg cgagccggca tcggcggcgt cggtcgccgg
     7081 gctcaggctc ctgcaccggc gcggcggcct ggagcgcggc ctcgtcgtcg tctgcgtcct
     7141 cacggggcac gg
//
LOCUS       DATLGG010000219         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_229641, whole genome shotgun sequence.
ACCESSION   DATLGG010000219 DATLGG010000000
VERSION     DATLGG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229641"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VM638_06235"
     CDS             <1..1039
                     /locus_tag="VM638_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12055.1"
                     /translation="LFDGFQRLGLAEYRLDLGSVAGDLATDRERRRYGGEVRILHVEE
                     RSRLRGLDPQPAVEDLYLTFVRRGGRWLVASDRDLEDVGLLSARRLWEIGPVEARREG
                     RFVYVAHPEVAEAAAAVIGSAEPAMQRVVERWPRPWHRRVAIIAPRDTDQLGRLIQAT
                     FDLDAFVAFAASGVERGADWDLVGHRILLNWPSFSSHAEGSRERILTHELLHVATRED
                     AGPMIPIFVEEGLAEWVTDERSGDVLGAVPARFDGRLPLDHEFTTGTPGEIRGAYLAS
                     ASAIGYAVERFGIERTVELYRRLGAVRREPGTWRYHTDRAMREAWGVGYRRFERDWVR
                     WVNGARADRAA"
     gene            1062..>2555
                     /gene="thrS"
                     /locus_tag="VM638_06240"
     CDS             1062..>2555
                     /gene="thrS"
                     /locus_tag="VM638_06240"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HVM12056.1"
                     /translation="MQDDLHVLRHSTAHVMAQAVCALWPEAKYAIGPPIQDGFYYDFE
                     LPVQLTPDHLPRIEKKMREIVKRDQPFVREEVPRDEALERFADQPFKTEIIEAVGEDE
                     EARAEAGAGDSVTVYRNDGWQDLCLGPHVDSTGKLGAFKLLSVAGAYWRGDENRPQLQ
                     RIYGTAWHTQEELDEYLHRLKEAEKRDHRKLGRDLDLFSFPDELGGGLSVWHPKGGVI
                     RKLLEDYVRDLHLERGYDLVATPHVARGGLWETSGHLEKYRDNMYPAMPAADGSEYFV
                     KPMNCPFHVLIYRSKTRSYRDLPMRLSELGTVYRYEKPGVLHGLLRVRGLTQDDSHIF
                     CTPDQLVDEIVKVFDLTLEIHRTFGFERPKVELSTKPGLAIGDESMWERATETLRDAL
                     ERSGLEFGIAEGEGAFYGPKVDFHFHDAIGRLWQLTTVQVDFALPERFGLEYAGEDNE
                     RHRPVMVHRAILGSVERFTGVLTEHYAGAFPLWLAPEQVRVIPIADRH"
BASE COUNT          380 a          866 c          950 g          359 t
ORIGIN      
        1 gctcttcgac gggttccagc gactgggcct ggccgagtac cggctcgacc tcggctcggt
       61 cgccggcgat ctcgcgaccg accgcgagcg tcgccgctac gggggcgagg ttcggatcct
      121 gcacgtcgag gagcgctccc ggctccgcgg cctcgacccg cagccggccg tcgaggacct
      181 gtacctgacg ttcgtgcgga gaggcggccg atggctggtg gcgtcggacc gggacctcga
      241 ggacgtcggg ctcctctccg cgcggcgcct ctgggagatc gggccggtcg aggcccggcg
      301 ggagggacgg ttcgtgtacg tcgcgcaccc ggaggtggct gaggcggccg cggccgtgat
      361 cggatccgcc gagccggcga tgcagcgggt cgtcgagcgg tggccgaggc cgtggcaccg
      421 acgggtggcg atcatcgccc cgcgcgacac cgaccagctg ggccggctga tccaggcgac
      481 gttcgacctc gacgcgttcg tggcgttcgc cgcgtcaggc gtggaacgcg gggcggactg
      541 ggacctggtc gggcaccgca tcctgctcaa ctggccgagc ttctcgagcc acgccgaggg
      601 ctcccgcgag cggatcctga cgcacgagct cctgcacgtc gccacccgtg aggacgccgg
      661 gccgatgatc ccgatcttcg tcgaggaagg cctggccgag tgggtcacgg acgagcgcag
      721 cggcgacgtc ttgggagcgg tcccggcgcg gttcgacggc cggttgccgc tcgaccacga
      781 gttcacgacc gggacgccgg gggagatccg gggggcgtac ctcgcctccg cctccgccat
      841 cggatacgcg gtcgagcggt tcgggatcga gcggacggtg gagctgtacc gacggctcgg
      901 cgcggtccgg cgcgagccgg gcacgtggcg ctaccacacc gaccgtgcca tgcgcgaagc
      961 gtggggcgtt ggctaccggc ggttcgagcg cgactgggtg cggtgggtca acggcgcgcg
     1021 cgcggaccga gccgcctgag cggcgggtag acttggcgac catgcaggac gacctccacg
     1081 tgctccgcca ctcgacggcg cacgtcatgg cgcaggcggt ctgcgcgctc tggccggagg
     1141 cgaagtacgc gatcggcccg ccgatccagg acggcttcta ctacgacttc gagctgccgg
     1201 tgcagctcac gcccgaccac ctcccgcgga tcgagaagaa gatgcgcgag atcgtgaagc
     1261 gggaccagcc gttcgtccgc gaggaggtcc cgcgggacga ggcgctcgag cgcttcgccg
     1321 accagccgtt caagaccgag atcatcgagg cggtgggcga ggacgaggag gcccgcgccg
     1381 aggcgggcgc gggagacagc gtcacggtct accgcaacga cggctggcag gacctctgcc
     1441 tcgggccgca cgtcgactct acggggaagc tcggcgcgtt caagctgctc tcggtcgccg
     1501 gcgcgtactg gcgcggggac gagaaccgcc cccagctcca gcggatctac gggaccgcct
     1561 ggcacacgca ggaggagctc gacgagtacc tgcaccggct gaaggaggcc gagaagcgcg
     1621 accatcggaa gctcgggcgc gacctcgacc tgttctcgtt cccggacgag ctcggtggag
     1681 ggctctcggt ctggcatccg aagggcggcg tgatccgcaa gctcctggag gactacgtgc
     1741 gggacctgca cctcgagcgc ggctacgacc tggtcgcgac gccgcacgtc gcgcgcggcg
     1801 ggctgtggga gacgagcggt cacctcgaga agtaccgcga caacatgtac ccggcgatgc
     1861 cggcggccga cggcagcgag tacttcgtca agccgatgaa ctgcccgttc cacgtcctga
     1921 tctaccgctc gaagacgcgc tcgtaccgcg acctgccgat gcggctatcc gagctcggca
     1981 ccgtgtaccg atacgagaag cccggggtgc tgcacgggct cctgcgcgtg cgcggcctga
     2041 cgcaggacga ctcgcacatc ttctgcacgc ccgaccagct cgtggacgag atcgtcaagg
     2101 tgttcgacct gacgctggag atccatcgca cgttcgggtt cgagcggccg aaggtggagc
     2161 tctcgacgaa gccgggcctc gcgatcggcg acgagtcgat gtgggagcgc gcgaccgaga
     2221 ccctgcgcga cgcgctcgag cgcagcggcc tcgagttcgg gatcgccgag ggcgaggggg
     2281 ccttctacgg gccgaaggtc gacttccact tccacgacgc gatcgggcgc ctctggcagc
     2341 tcacgaccgt gcaggtggac ttcgcgctgc cggagcgctt cggtctcgag tacgcgggcg
     2401 aggacaacga gcgtcaccgt cccgtgatgg tccaccgggc gatcctcggt tcggtcgagc
     2461 ggttcaccgg cgtgctgacc gagcactacg ccggggcatt cccgctctgg ctggcgccgg
     2521 agcaggtgcg cgtcatcccg atcgccgacc gtcac
//
LOCUS       DATLGG010000220         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_151470, whole genome shotgun sequence.
ACCESSION   DATLGG010000220 DATLGG010000000
VERSION     DATLGG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151470"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VM638_06245"
     CDS             <1..915
                     /locus_tag="VM638_06245"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HVM12057.1"
                     /translation="DTSDDWIVSRTGIRARHFVGEGETASSLAAQAARNALDAAGIDP
                     SAIDLTIVATVSGDQPLPSTASFVQDHLGLSGGAFDLAAACAGFIYGTELAAAQIEAG
                     RAETVLVCGTEVLSRFVNFRDRTTCVLFGDGAGAAVLTPGDEPGLIGSILQNDGSHAR
                     ILEVPAGGSAEPVTEETIREARDKIRMPDGREVFKRAVVGMSDAAAALLDKKGFSADD
                     VDLLVPHQANVRIIKSVAQRLQFPMEKVFVDLEEVGNTSAASVPIALDHAWRSGRLQP
                     GNLVLTAAFGAGLAWGANLLRWTAEAPR"
     gene            912..1649
                     /gene="fabG"
                     /locus_tag="VM638_06250"
     CDS             912..1649
                     /gene="fabG"
                     /locus_tag="VM638_06250"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HVM12058.1"
                     /translation="MTERVALVTGASGGIGRATAMALARSGAAVACGYATNDAGAKET
                     VALIEEAGGTAAAFGGDIADPSAVEDLFRQVAEWRGAPLIVVNNAGTNRDGLAVKFPP
                     QEFARILTVNLTGAFLCIQQSLPAMLKARWGRIINVSSVVAIRGNAGQPAYSAAKSGL
                     LGLTRSLAKEYGRRGITVNAVCPGYVQTDMTSSLDERQRQDLRGVIPAGRVGEAEEIA
                     AAIAFLASEEASYVNGVVLPVDGGMTA"
     gene            1671..1922
                     /gene="acpP"
                     /locus_tag="VM638_06255"
     CDS             1671..1922
                     /gene="acpP"
                     /locus_tag="VM638_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVM12059.1"
                     /translation="MERQEVEERVRKVLAEQLSVDLEKVTPDARFEEDLDADSLDLVE
                     AVLGLEEELGVDIPEEEMEGIKTVGQAIDLAMGKLEASA"
     gene            1919..3145
                     /gene="fabF"
                     /locus_tag="VM638_06260"
     CDS             1919..3145
                     /gene="fabF"
                     /locus_tag="VM638_06260"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HVM12060.1"
                     /translation="MTRKVVVTGIGPVSPVGVGREAMWSSILAGRSGIGPVTLFDVSE
                     HAVKIAGEIRDFDAEAFMDRKAVRRTDRAVQFAVGAAKLAWEDAGSPQADPSRTAVVV
                     STGIGGLTTLLEQTRILWEKGPGRVSAFMVPGMMPNAAAGQVAMEFGFTGPNTCITTA
                     CAAGAHGVGEAYRYIKHGLADVALAGGTEAVITDLAMAGFSQMQALSRNPDATRASRP
                     FDADRDGFVLSEGACILVLEEEERARARGATIYAEVAGYGASADAHHITAPEPEGSGA
                     VMAIQAALADAGEPAEAVDYVNAHGTSTPLNDAAETKAIKKALGDHAHRIAMSSTKSM
                     TGHLLGAAGALESAISALAIHERMLPPTINLQTPDPECDLDYVPNEARPAEVRLALNE
                     AFGFGGHNAVVAFRKA"
     gene            complement(3226..>4107)
                     /locus_tag="VM638_06265"
     CDS             complement(3226..>4107)
                     /locus_tag="VM638_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12061.1"
                     /translation="YRVRCFYKGPSASNLSEFTGDCPQSPQTGMYGGHAFDPPGNQGP
                     WPTATGAMFEIWIPVKSSRQLNGSVPSSGPCDTCLYAGVWMIDGVNSPWVWPRIPVYV
                     GGSSTTSGPYVTYPAPAITDVTYNPSDNAAYVKLWANLFTEGTSGKAYFQMGFKKGEY
                     VAKSNPEITVPSGSWQMSEIWRMQPGKIYHWRACYEPTGGTRVCGPNKSFHAPPDTVI
                     TESKIRRSKRRATFSFTSPTSGMIFQCKLDDQKWKACTSPRAYGKLSKGTHVFRVRAV
                     DPVGNKDTTPAKKAFRI"
BASE COUNT          617 a         1398 c         1477 g          615 t
ORIGIN      
        1 gacacgagcg atgactggat cgtctcgcgc accgggatcc gagcgcgcca cttcgtcggg
       61 gagggagaga cggcctcgtc cctcgcggcc caggcggcgc ggaacgcgtt ggacgccgcc
      121 gggatcgacc cctcggcgat cgacctgacg atcgtcgcga ccgtcagcgg ggatcagccc
      181 ctgccgtcga ccgcctcctt cgtccaggac cacctcgggc tctcgggagg cgcgttcgac
      241 ctcgctgccg cgtgcgcagg cttcatctac gggaccgagc tggccgccgc gcagatcgaa
      301 gcgggccgcg ccgagacggt cctcgtctgc ggcaccgagg tgctctcgcg gttcgtgaac
      361 ttccgcgacc ggaccacctg cgtgctcttc ggcgacgggg ccggcgccgc cgtcctgaca
      421 cccggtgacg agccgggcct gatcggatcg atcctgcaga acgacggctc ccacgcgcgg
      481 atcctcgagg tgccggcggg tggttcggcc gagccggtga ccgaggagac gatccgcgag
      541 gcccgcgaca agatccggat gccggacggg cgcgaggtct tcaagcgcgc cgtcgtcggg
      601 atgtccgatg cggccgctgc gctcctcgac aagaaggggt tctccgcgga tgacgtggac
      661 ctgctcgttc cccatcaggc gaacgtgcgg atcatcaagt cggtggctca gcggctccag
      721 ttcccgatgg agaaggtgtt cgtggacctc gaggaggtcg gcaacacctc ggcggcgagc
      781 gtcccgatcg cgctcgatca cgcgtggcgc agcgggcgtc tccagccggg gaacctcgtg
      841 ctcaccgccg cgttcggagc cggcctcgcc tggggcgcga acctgctgcg gtggacggcg
      901 gaggcgccgc ggtgacggag cgcgtcgcgc tcgtgaccgg cgcctcgggc ggcatcggcc
      961 gcgcgaccgc gatggccctc gcgcgctccg gggctgcggt cgcgtgcggg tacgccacga
     1021 acgacgccgg cgcgaaggaa acggtcgcgc tgatcgagga ggctggcggc acggccgcgg
     1081 cgttcggcgg cgacatcgcc gacccctccg ccgtcgaaga cctcttccgg caggtcgccg
     1141 agtggcgcgg cgctccgctc atcgtggtca acaacgcggg caccaaccgc gacgggctcg
     1201 cggtcaagtt ccccccgcag gagttcgcca ggatcctcac ggtgaacctg accggcgcgt
     1261 tcctgtgcat ccagcagagc ctcccggcga tgctgaaggc gcgctggggg cggatcatca
     1321 acgtctcctc ggtcgtggcg atccgcggca acgccggcca gccggcgtac tcagcggcga
     1381 agagcgggct cctcgggttg acccgctccc tcgcgaagga gtacgggcgc cgcgggatca
     1441 cggtgaacgc cgtgtgcccg ggctacgtgc agaccgacat gacctcctcg ctcgacgagc
     1501 ggcagcggca ggaccttcgc ggggtgatcc ccgccggccg cgtcggcgag gcggaggaga
     1561 tcgcggccgc gatcgcgttc ctcgcgagcg aagaggcctc gtacgtgaac ggagtggtgc
     1621 tccccgtgga cggcgggatg accgcctagg ctgaggaaga ggaggacgac atggagcggc
     1681 aggaagtcga agagcgggtg cggaaggtgc tggccgagca gctctcggtc gacctcgaga
     1741 aggtgacgcc ggacgcgcgg ttcgaggagg acctcgacgc ggactcgctc gacctcgtcg
     1801 aggccgtcct cgggctcgag gaagagctcg gcgtggacat ccccgaggag gagatggagg
     1861 ggatcaagac ggtcgggcag gccatcgacc tcgcgatggg gaagctggag gcgtccgcgt
     1921 gacgaggaag gtcgtcgtca ccgggatcgg ccccgtctcc cctgtcggcg tcggccgcga
     1981 ggcgatgtgg tcctcgatcc tcgccggacg gtccgggatc ggcccggtga cgttgttcga
     2041 cgtctcggag catgcggtca agatcgcggg cgagatccgc gacttcgacg ccgaggcgtt
     2101 catggaccgc aaggcggttc gacggaccga ccgcgcggtg cagttcgcgg tcggggcggc
     2161 gaagctcgca tgggaggacg cgggaagccc ccaggccgat ccctcgcgca ccgccgtcgt
     2221 cgtgtccacc ggcatcggtg gtctcacgac gctcctggag cagacgcgga tcctctggga
     2281 gaagggcccc gggcgggtct ctgccttcat ggtgcccggc atgatgccga acgccgcggc
     2341 cggacaggtc gcgatggagt tcggcttcac ggggccgaac acctgcatca cgaccgcatg
     2401 cgcggccggc gcccacggcg tcggtgaggc gtaccgctac atcaagcacg gcctcgccga
     2461 tgtggcgctg gccggcggga ccgaggcggt catcaccgac ctcgcgatgg ccggcttcag
     2521 ccagatgcag gcgctctcgc ggaacccgga cgcgacgaga gcgtcccgtc cgttcgacgc
     2581 cgaccgcgac gggttcgtcc tgtcggaggg ggcgtgcatc ctcgtcctcg aggaggagga
     2641 gcgtgcgcgg gcgcgcggcg cgacgatcta cgccgaggtc gccgggtacg gcgcctccgc
     2701 cgacgcccac cacatcacgg cgccggagcc ggagggaagc ggcgcggtca tggcgatcca
     2761 ggccgcgctc gcggacgccg gcgagccggc tgaggccgtc gactacgtga acgcgcacgg
     2821 gacgtcgacg ccgctcaacg atgcggccga gacgaaggcg atcaagaagg cgctcggcga
     2881 ccacgcgcac cggatcgcga tgtcgtcgac gaagtcgatg accggccacc tgctcggcgc
     2941 agccggggcg ctggagtctg cgatctccgc gctcgcgatc cacgagcgga tgctgccgcc
     3001 gacgatcaac ctccagaccc ccgatccgga gtgcgacctc gactacgtgc cgaacgaggc
     3061 ccgtcccgcc gaggtgcgtc tcgcgctcaa tgaggccttc ggcttcggcg gccacaatgc
     3121 cgtcgtcgcc ttccggaagg cctgagcggc ccagcgcaca ggccgggacc cggcggacgc
     3181 cgccactcct ggcctgggcc gtccgcggga ccgagctgca accggctaga tccggaacgc
     3241 cttcttggcc ggtgtcgtgt ccttgttccc gacgggatcc acggctcgaa cacggaacac
     3301 gtgcgtcccc ttcgacagct tgccgtaggc cctcggcgac gtacacgcct tccacttctg
     3361 gtcgtcgagc ttgcactgga agatcatccc gctcgtcgga gaggtgaagc tgaaggtggc
     3421 ccgtcgcttg gatctcctga tcttcgactc cgtgatgacg gtgtcgggtg gcgcgtggaa
     3481 cgacttgttc ggcccgcaca ccctggtgcc accggtgggc tcgtagcagg ctcgccagtg
     3541 gtagatcttt ccgggctgca tccgccagat ctccgacatc tgccagctcc ctgatggaac
     3601 cgtgatctcc gggttgctct tggcgacgta ctcccccttc ttgaagccca tctggaagta
     3661 cgctttgccg ctcgttccct cggtgaagag gttcgcccaa agcttcacgt acgccgcgtt
     3721 atcggagggg ttgtaggtga catcggtgat cgccggcgca gggtaggtca cgtacgggcc
     3781 gctcgtggtc gagctcccgc cgacgtagac agggatgcgc ggccagaccc aggggctgtt
     3841 gacaccgtcg atcatccaca cgcccgcgta caggcaggtg tcgcaaggcc cgctggatgg
     3901 gacgctcccg ttcagctgac ggctcgactt cacggggatc cagatctcga acatggctcc
     3961 ggtggccgta ggccagggtc cctggttccc cggcggatcg aacgcatggc ctccgtacat
     4021 ccccgtctgc gggctctgcg ggcaatcccc cgtgaactct gagaggttgc tcgccgaggg
     4081 gcccttgtag aagcaccgga cccggta
//
LOCUS       DATLGG010000221         8843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_151487, whole genome shotgun sequence.
ACCESSION   DATLGG010000221 DATLGG010000000
VERSION     DATLGG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151487"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VM638_06270"
     CDS             <1..640
                     /locus_tag="VM638_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HVM12062.1"
                     /translation="YHASKVHDSTNIHLACRIWGLRATDLNQGVVYGIETDETRMDER
                     FHTRFDYDEVWGTALNRFCLQAVIGHPLTVYGAGGQTRGFLNIVDTLQCVELATLNPA
                     DVGEYRVFNQFTETFSLLELARLVERAAGEYGLRVRVDHVPNPRVEAEQHYFNPVHTK
                     LLDLGLKPHLLSETLIGSMFGAIERYRERVIPEAIMPRTRWAPGTGGDRARR"
     gene            618..1688
                     /locus_tag="VM638_06275"
     CDS             618..1688
                     /locus_tag="VM638_06275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12063.1"
                     /translation="MGTELAARLVALRLIGGLFAVALLIWSTVRFRKRRISRLNLLIS
                     WAIGLTIALVALWPDLFNPVFGLFNFRRGGGQRIVGLLLLGNLVLLALVLRNLTQTDL
                     VTRDLRLLIEVLGTRSFDETKVADLPAGERIVVVMPAFNEAENVGAVIRAMPAEVEGR
                     PVVALVVDDASTDGTDAVAEEAGALVARLPVRRGGGLALRVGYEIALKLGAVVVASMD
                     ADGQHLPSELPQVVGPVLRGEADMVQGSRVLGAFERESRIRHLGVGFFSGLVSLMTGV
                     RVTDISNGYRATRTDTLRKLVLEQDQFWTSEILLEGLRQHARIVEVPVTVRARAGGVS
                     KKPKSLRYGWNFTKAIFQTWLR"
     gene            1676..3514
                     /locus_tag="VM638_06280"
     CDS             1676..3514
                     /locus_tag="VM638_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12064.1"
                     /translation="MAPLGARARAGVGPALVVLVGFGVFLSIYVVRGIEGAIGWDATQ
                     YAWRGELAAAVGLDALPDRLPSGIEVKAGRPGFPAVAGPAGALLGASMLDMTAILPVA
                     LALAVGLAGAAVAVPLVGDERWGFAALGVVAVVSPNVVLMLRYAYLDNLLVTAIFLAA
                     AAVAVLAVRDRRRLIPAVALHAAAGLAHPPFVWVTSAILVAAAAVLLRRSGRERAEGR
                     GRLDTSAARLLVVGVGGAVASLVLRLALAVGTASPVLERSGFVERLGNDLGAYLLPVT
                     IPLAVAGAVLALHRLRGPSPASPPTLALLLGWSGMTIAAVGAFVLLPLPIPANRVLVF
                     ALAIPLLAAVGVILLAGGLRGWLRPAAVVMVVSALALVGTWRWINTAPQTDPARLREA
                     AWLAAYLEAARIPPDREVVVIVDDRSEDPATRTMLMVDHLLAALPPGRMGQIHPYLGS
                     TASFLAGEPSEGPGAYRRASRRFLERIDPASAVAVVLPSYRREPGRGPADPRPGEVVQ
                     GPARPPGLDVEDPVIGTPRLAVLALATLLLLGLAGGGWARRLVGGDDAVRAVALAPAI
                     GAAGILLVTLAADRFGLGIASPGAAAIPVLVGAAGWVAAARKRRPA"
     gene            3511..5550
                     /locus_tag="VM638_06285"
     CDS             3511..5550
                     /locus_tag="VM638_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12065.1"
                     /translation="MSVLALFVLLLLVLVPGTGALLAFDPTARLSSTTRLALAFALGY
                     AIPGAIAFLLVLLSIFSAPLFLALLGVATLACWTVAVRRGAPRAHLQAVREEVRTERW
                     PLVLGLAALAAFAVVRSGYLGDFPAGTPYRYWVDGMEVADAGGIPPSTLHHGALWPPV
                     TSKMFLNAFTAGAHVLTGWSVPAAIVAMLWVTAIGSFVGLWATARELGLRWVAPLVPV
                     LLLANRAIFGDEMTLDLLEYRAEIVGRLVVFCALPLGIVALRDRIRRRDAVVTGLLLA
                     AAAGTHLVALTAGVIVLACYGLARLVLDRERRPVLRQGLLLGGITIVVAAAVLILPRG
                     EIGFGGAADPSQYAATDVTFDKSRFLYSGSLAPADHPPRGRFYVPPGGVLAAFVQEGL
                     RPPPGSILTGPAGTMALALGGLALALLVFLRFPRELRPIGPIAVGLGGILAGAALFFS
                     WRYDVFIPATFGIRRLTDYAAVPVVLLLSGVAEVGLRAMRRVGPRGPVAGATVLVVLV
                     TLLVLPSGRVTAEQVRRNHLAREAFAWIRSEVPCDARILLNQRTVGAVRALTGRVAVL
                     EGMGPFLRPRMLDRVVETLLGARAFFVEPAANRAFLEEHGITHVILVKRFRLGYSPEA
                     GGRPRPGRLRDVPFLEPMHRGSSFDAYRVVGVQGRGRFPDPSAHPAYDCRRDPLR"
     gene            5550..7505
                     /locus_tag="VM638_06290"
     CDS             5550..7505
                     /locus_tag="VM638_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12066.1"
                     /translation="MLPAPSGPLVAGVTGAVVAAGFLGLGFLVVRALAGARPIERTLA
                     WSLSLPGAVAFTLILTLVHIVSGGRVFASPWLVRGLTVAAALGLWLAVRRRQPAEEDR
                     WDVRLALLLAGAGLLVWGLPVVRLLPLDFFGDTHWHAGWAAQMLNGEPLPSAAITGEI
                     PNYYPWLFHAACAFLATFTPGGTTLHALAPLQMALVVGTVLILFRLGRSLTGLRATGG
                     WLALFAGMSGGLGFVLLRYRDVVIWPRFEEGPTVAALRYGGDLLYRRSYHVAFSNLSP
                     PLPRDVALAMLLGFLLLVAVGLRRRSVAALVGAGACLGMAGIAGAEAFLVGCAAAVGL
                     TFVPVTLPRRTVALAVLGPAAALAAIWLVPLAIGYTRLGGFVNITGVEAIDLPPWSIL
                     VAWGITTPLAVVGVLRWRTLRGRPGGRVALAVVAGALFLLFAATLIPGAVGRAFLTLG
                     RRHRYWPMLHLGVTMLASVGVTHLLGRIASRHRRLVASAIVVALAIPSAFVASISAPL
                     AVPQPPAVAAALRGEPGTFVDALRGPTGARCTVAVPGRERQAIVWSLTGYRLVSADWK
                     TRLPRNRARIRWRDLVPDDDARRRDNAALVTGDVDPQRWRDLVEGYAVDAILVEPQHA
                     SAPVFTGYPTTRTREGPVVVRTGSCDG"
     gene            complement(7510..8142)
                     /gene="thiE"
                     /locus_tag="VM638_06295"
     CDS             complement(7510..8142)
                     /gene="thiE"
                     /locus_tag="VM638_06295"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVM12067.1"
                     /translation="MKPAKRRDRLAAARLYLVTGGRRPDIVPFLEAVLAAGVDIVQLR
                     EKDAEAGEVMRLAEPFVEAAERHGALFVVNDRADIAAAVGADGLHVGQDDLPVAAARE
                     LLGPEVLIGLSTHSASQLDAAPAEADYLCAGPVWETPTKPGRPAAGLAAVRHAAATAR
                     RPWFAIGGIDTGNLPSVVDAGARRIVVVRAITQAPDAAEAVRALRAGLPD"
     gene            complement(8139..8339)
                     /gene="thiS"
                     /locus_tag="VM638_06300"
     CDS             complement(8139..8339)
                     /gene="thiS"
                     /locus_tag="VM638_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HVM12068.1"
                     /translation="MPRIVANGREYEVSETTVAGFVRSLGLDPRVVIVERNGEALERA
                     RYEEVELAEGDRLEVVRAVAGG"
     gene            complement(8341..>8843)
                     /locus_tag="VM638_06305"
     CDS             complement(8341..>8843)
                     /locus_tag="VM638_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12069.1"
                     /translation="RHDRLGALYGLADDWRAEAIVTAAHARFLMRMYWRAQVTVSVPR
                     IRPSASGFQPRVEVGDRDLAQIVCAMRLGMPDAGIVLSTREPAGLRDGLHRIGVTHTS
                     AGSHTEPGGYEHPGEAEEQFEIADERSPAEVAAALRAMGYDPVWGDPSTPEGMARALR
                     GTAAVG"
BASE COUNT          908 a         3384 c         3176 g         1375 t
ORIGIN      
        1 ctaccacgcc tcgaaggtcc acgactcgac gaacatccac ctcgcgtgcc gcatctgggg
       61 cctgcgcgcc acggacctca accagggcgt cgtctacggg atcgagaccg acgagacgcg
      121 gatggacgag cgcttccaca cgcgcttcga ctacgacgag gtgtggggca ccgcgctgaa
      181 ccggttctgc ctccaggccg tgatcggcca tccgctcacg gtctacggcg ccggcgggca
      241 gacccgcggc ttcctgaaca tcgtcgacac gctccagtgc gtcgagctcg cgacgctcaa
      301 cccggcggac gtgggcgagt accgcgtgtt caaccagttc accgagacct tctcgctcct
      361 cgagctggcg cggctcgtgg agcgcgccgc cggggagtac ggcctgcgcg tgcgcgtcga
      421 ccacgtgccg aacccgcggg tggaagccga acagcactac ttcaacccgg tgcacacgaa
      481 gctgctcgac ctcggcctga agccgcatct gttgtcggag acgttgatcg ggtcgatgtt
      541 cggggcgatc gagcggtacc gcgaacgcgt gatccccgag gccatcatgc cgcggacgcg
      601 ctgggctccc gggacgggtg gggaccgagc tcgccgctag gctcgtcgcc ctccgcctga
      661 tcggagggct cttcgccgtc gccctcctga tctggtcgac ggtccgcttc cgcaagcgtc
      721 gcatcagccg gctcaacctg ctgatctcct gggcgatcgg gctgacgatc gcgctcgtcg
      781 cgctctggcc ggacctgttc aacccggtct tcgggctctt caacttccgc cgcggcggcg
      841 gccagcggat cgtcggactg ctcctgctcg gcaacctcgt cctgctcgcg ctcgtgctgc
      901 gcaacctgac gcagaccgac ctcgtcacgc gggacctgcg cctgctgatc gaggtcctcg
      961 ggacacgatc gttcgacgag acgaaggtgg cggaccttcc cgccggcgag cggatcgtcg
     1021 tcgtcatgcc cgcgttcaac gaggccgaga acgtgggcgc ggtgatccgc gcgatgccgg
     1081 ccgaggtcga gggtcggccg gtcgtggcgc tcgtcgtcga cgacgcctcg accgacggca
     1141 ccgacgcggt cgccgaggag gccggcgcgc tcgtggcgcg cctgcccgtg cgccggggcg
     1201 gcgggctcgc cctgcgcgtc ggctacgaga tcgcgctgaa gctcggcgcc gtcgtcgtcg
     1261 cctcgatgga cgccgacggc cagcacctgc cgagcgagct gccccaggtc gtggggcccg
     1321 tcctccgcgg cgaggccgac atggtgcagg gctcgcgtgt gctcggcgcg ttcgagcgcg
     1381 agagccggat ccgccacctc ggcgtcggct tcttctcggg gctcgtctcg ctcatgacgg
     1441 gcgtgcgcgt caccgacatc tcgaacggct accgcgcgac gcggaccgac accctgcgca
     1501 agctcgtcct cgagcaggat cagttctgga cctccgagat cctcctcgag gggctgcgcc
     1561 agcacgcgcg gatcgtggag gtcccggtga cggtccgcgc gcgggcgggg ggcgtgtcga
     1621 agaagccgaa gagcttgcgc tacggctgga acttcacgaa ggcgatcttc cagacgtggc
     1681 tccgctaggg gcgagggcgc gcgccggcgt cgggcccgcg ctcgtcgtgc tggtcggctt
     1741 cggcgtgttc ctctcgatct acgtcgtccg gggcatcgag ggggcgatcg gctgggacgc
     1801 gacgcagtac gcgtggcgcg gcgagctcgc ggcagccgtc ggcctcgatg ccctccccga
     1861 ccggctgccg agcggcatcg aggtcaaggc gggtcggccc gggttcccgg cggtcgccgg
     1921 cccggccggc gccctcctcg gtgccagcat gctcgacatg acggcgatcc tcccggtcgc
     1981 gctcgctctc gcggtcggcc tcgccggggc cgcggtcgcc gttccgctcg tcggtgacga
     2041 gcgctggggg ttcgcggcgc tcggcgtggt cgcggtggtt tcgccgaacg tcgtgctgat
     2101 gctgcggtac gcctacctcg acaacctcct cgtcaccgcg atcttcctcg ccgccgcggc
     2161 ggtggcggtc ctcgccgtcc gcgaccggcg acggctcatc ccggcggtgg cgctccacgc
     2221 ggccgcgggt ctggcgcatc cgccgttcgt ctgggtcacc tcggcgatcc tcgtggcagc
     2281 ggccgccgtg ctcctgcggc gctcggggcg agagcgcgcg gagggccgcg ggcgcctcga
     2341 tacctcggcg gcgcgtctcc tcgtcgtcgg ggtcggcggc gccgtcgcca gcctcgtcct
     2401 gcgcctcgcg ctcgcggttg ggaccgcttc gccggtgctc gagcggtccg ggttcgtgga
     2461 acgactcggg aacgacctcg gggcgtacct cctgcccgtg acgatcccgc tcgccgtcgc
     2521 cggcgccgtc ctggcgctgc atcggctgcg cggcccgagc cctgcatcac ccccgacgct
     2581 cgcgctcctg ctggggtgga gcgggatgac gatcgcggcg gtcggcgcgt tcgtgctcct
     2641 gccgctcccg atcccggcga accgcgtgct ggtcttcgcg ctcgcgatcc ccctcctcgc
     2701 ggcggtcggc gtgatcctgc tcgccggagg cctccgcggc tggctccggc cggcggccgt
     2761 cgtcatggtc gtgtccgcgc tcgccctcgt cgggacgtgg cggtggatca acaccgcgcc
     2821 gcagaccgac cccgcgcgcc tccgcgaggc ggcgtggctc gcggcgtacc tcgaggcggc
     2881 ccggatcccg ccggaccgcg aggtcgtcgt gatcgtcgac gaccggtccg aggatccggc
     2941 gacgcggacg atgctcatgg tcgaccacct cctcgccgcg ctgccgccgg ggcgcatggg
     3001 gcagatccat ccgtacctcg gctcgaccgc gagcttcctc gccggtgagc catccgaggg
     3061 tccgggcgcg taccgccgcg cttctcgtcg cttcctcgag cggatcgacc cggcctcggc
     3121 ggtggccgtc gtcctgccct cgtaccggcg ggagccgggt cgaggaccgg cggatccgcg
     3181 gccgggcgag gtcgtgcagg ggccggcgcg gccgcccggg ctcgacgtcg aggacccggt
     3241 gatcggaacg ccgcgtctgg ctgtgctcgc gctcgcgacc ctgctgctgc tcgggctcgc
     3301 gggcggcgga tgggcgcggc gcctcgtggg cggcgacgac gccgtgcgcg cggtggccct
     3361 cgcccccgcg atcggggcgg ccggcatcct cctcgtcacc ctcgcggcgg accggttcgg
     3421 cctggggatc gcctcgcccg gagcggcggc gatcccggtc ctcgtcgggg ccgccggctg
     3481 ggtggccgcg gcgcggaagc ggaggccggc gtgagcgtcc tcgcgctctt cgtgctcctg
     3541 ctgctcgtcc tcgtgcccgg gaccggcgcc ctcctcgcgt tcgatcccac ggcccgtctc
     3601 tcctcgacga cgcgcctcgc gctcgcgttc gccctcggct acgcgatccc cggcgcgatc
     3661 gccttcctcc tcgtcctgct ctcgatcttc tccgcaccgc tgttcctcgc cctcctcggc
     3721 gtcgcgacgc tcgcctgctg gaccgtcgcg gtccggcgcg gggcgccgcg ggcgcacctg
     3781 caggcggtcc gcgaggaggt ccgcacggag cggtggccgc tcgtgctcgg gctcgccgcg
     3841 ctcgccgcct tcgcggtcgt ccgcagcggc tacctcggcg acttcccggc cggcacgccc
     3901 taccgttact gggtcgacgg gatggaggtc gccgacgcgg gcgggatccc gccgagcacg
     3961 ctgcaccacg gggcgctctg gccaccggtg acgagcaaga tgttcctcaa cgcgttcacc
     4021 gccggggccc acgtcctcac cgggtggagc gtccccgcgg cgatcgtcgc gatgctctgg
     4081 gtgacggcga tcgggtcgtt cgtcgggctc tgggcgaccg cccgtgagct cggccttcgg
     4141 tgggtcgcgc cgctcgtccc ggtactgctg ctcgcgaacc gggcgatctt cggcgacgag
     4201 atgaccctcg acctgctcga gtaccgggcc gagatcgtcg ggcgcctcgt ggtcttctgc
     4261 gcgctgccgc tggggatcgt cgcgctgcgc gaccgcatca ggcgccgcga cgcggtggtc
     4321 acgggcctcc tgctggccgc cgccgccggc acgcacctcg tcgccctgac cgcgggcgtc
     4381 atcgtcctgg cctgctacgg gctcgcgcga ctcgtgctcg accgggagcg tcggcccgtc
     4441 ctccgccagg gcctcctgct cggcggtatc acgatcgtcg tggccgccgc cgtcctcatc
     4501 ctcccgcgcg gcgagatcgg gttcgggggg gccgccgacc cgtcgcagta cgcggcgacg
     4561 gacgtcacct tcgacaagtc ccggttcctc tactccggga gcctcgcgcc ggccgatcac
     4621 ccgccgcggg gacgcttcta cgtcccgccg gggggcgtcc tcgccgcctt cgtccaggag
     4681 ggtctgcggc cgccgccggg gtcgatcctc accggccccg cgggaacgat ggcgctcgcg
     4741 ctcggcggtc tcgcgctcgc cctgctggtg ttcctccgct tcccccggga gctccggccg
     4801 atcggcccga tcgcggtcgg cctcgggggg atcctcgccg gagcggcgct cttcttctcc
     4861 tggcggtacg acgtcttcat cccggcgacg ttcgggatcc gccggctgac ggactacgcc
     4921 gccgtcccgg tcgtgctgct cctctccggt gtggccgagg tcgggctccg ggcgatgcgg
     4981 cgcgtcggac cgcgcgggcc ggtggccgga gcgacggtcc tcgtggtgct cgtgacgctc
     5041 ctcgtccttc ccagcgggcg cgtgacggcc gagcaggtgc ggcgcaacca cctcgcgcgg
     5101 gaagccttcg cctggatccg gtcggaggtt ccctgcgacg cgcgcatcct gctgaaccag
     5161 cggacggtcg gggcggtgcg cgcgctcacc ggccgcgtcg cggtgctcga ggggatgggg
     5221 ccgttcctcc gaccgcggat gctggaccgc gtcgtcgaga cgctgctcgg cgcgcgcgcc
     5281 ttcttcgtcg agccggccgc caaccgcgcg ttcctcgagg agcacggcat cacgcacgtg
     5341 atcctcgtga agcggttccg gctcggatac tcacccgagg cgggagggcg cccgcggccg
     5401 ggacggctcc gggatgtccc cttcctcgag ccgatgcacc gcggctcttc gttcgacgcg
     5461 taccgcgtgg tcggcgtgca gggccgcggc cgcttccccg acccatccgc gcacccggcc
     5521 tacgactgcc gccgggatcc gctccgttga tgctgcccgc gccgtcgggg ccgctcgtcg
     5581 ccggcgtgac gggcgcggtc gtcgcggccg ggttcctcgg gctcggcttc ctcgtggttc
     5641 gcgccctcgc cggcgcgaga cccatcgagc gcacgctggc gtggagcctc tcgctccccg
     5701 gggcggtggc cttcaccctc atcctcacgc tcgtccacat cgtcagcggc gggcgcgtct
     5761 tcgcgtcgcc ctggctggtc cgcgggttga ccgtcgcggc ggccctcggg ctctggctcg
     5821 cggtacggcg gcgacagcca gccgaagagg atcggtggga cgtccgcctc gcgctcctgc
     5881 tcgcaggtgc cgggctcctc gtctgggggt tgccggtcgt ccgactgctc cccctcgact
     5941 tcttcggcga cacgcactgg cacgccggct gggcggcgca gatgctgaac ggcgagcccc
     6001 tgccctccgc cgcgatcacc ggcgagatcc cgaactacta cccgtggctc ttccacgcgg
     6061 cctgtgcttt cctcgcgacg ttcacccccg gcggcacgac cctgcacgcg ctggcgccgc
     6121 tccagatggc gctcgtcgtg gggaccgtcc tgatcctgtt ccggctcggc cgctcgctca
     6181 ccgggctgcg ggcgaccggc gggtggctgg cgctcttcgc cggcatgtcg ggagggctgg
     6241 ggttcgtcct cctgcgctac cgagacgtcg tgatctggcc gcggttcgag gagggtccga
     6301 ccgtcgccgc gctgcgctac ggcggagacc tgctctaccg ccgctcgtac cacgtcgcgt
     6361 tctcgaacct ctcgccgccg ctcccccgcg acgtcgcgct cgcgatgctg ctcggcttcc
     6421 tgctgctcgt cgccgtcggc ctccgccgtc ggagcgtggc cgcgctcgtg ggcgcgggtg
     6481 cgtgcctcgg tatggcaggc atcgccgggg ccgaggcgtt cctcgtcggg tgcgctgcgg
     6541 cggtgggtct cacgttcgtg cccgtgacgc tcccgcggcg gaccgtcgcg ctcgccgtcc
     6601 tcggacctgc ggccgccctc gcggcgatct ggctcgtgcc gctcgcgatc gggtacacgc
     6661 gcctcggcgg gttcgtgaac atcacggggg tcgaggcgat cgacctcccg ccgtggtcga
     6721 tcctcgtcgc ctgggggatc acgacgccgc tcgccgtggt cggggtgctg cgatggcgga
     6781 cgcttcgagg ccgcccgggt ggccgggtgg cgctcgcggt cgtcgcggga gccctgttcc
     6841 tcctgttcgc agcgaccctg atccccggcg ccgtggggcg cgcgttcctc acgctcggcc
     6901 ggcgacaccg gtactggccg atgctgcacc tcggcgtcac gatgctcgcg tcggtgggcg
     6961 tcacgcacct cctcggacgc atcgcgagca gacaccggcg cctcgtcgcc tcggcgatcg
     7021 tcgtcgcgct cgcgatcccc tcggcgttcg tggcgtcgat ctcggcaccg ctcgcggtcc
     7081 cgcagcctcc cgcggtcgcc gcggcgctcc ggggcgagcc ggggacgttc gtggacgccc
     7141 tccgcggccc gaccggagcc cgatgcaccg tcgcggtgcc cggccgggag cgacaggcga
     7201 tcgtctggtc gctcacgggc taccgcctcg tctcggccga ttggaagacg cgtctcccgc
     7261 gcaaccgcgc ccgcatccgg tggcgggacc tcgtcccgga cgacgacgcg cggcgacgcg
     7321 acaacgccgc gctcgtcacg ggcgacgtcg acccccagcg gtggcgggac ctcgtcgagg
     7381 ggtacgcggt cgatgcgatc ctcgtcgagc cgcagcatgc gagcgctccg gtgttcaccg
     7441 ggtacccgac gacgcgcacg cgcgaggggc cggtcgtcgt tcgcaccggg tcctgcgacg
     7501 gctgatcgat cagtccggga gacccgcccg cagcgcccgg accgcctctg cggcgtcggg
     7561 cgcctgcgtg atcgcgcgga cgaccacgat ccgccgagcg ccggcgtcca cgaccgatgg
     7621 caggttcccc gtgtcgatcc cgccgatcgc gaaccacggt ctcctggcgg tggcggccgc
     7681 gtggcgcacc gccgccagcc ccgcggcggg tcgcccgggc ttcgtcggcg tctcccacac
     7741 cggccccgcg cagaggtagt cggcctccgc cggggcggcg tcgagctgcg acgcggaatg
     7801 cgtcgacagc ccgatcagca cctcggggcc gaggagctcg cgtgcagccg ccaccggcag
     7861 gtcgtcctgg ccgacgtgca gcccgtccgc gccgacggca gcggcgatat ccgcccggtc
     7921 gttcacgacg aacagcgcgc cgtgtcgctc ggcggcctcg acgaacggct cagcgaggcg
     7981 catcacctcc ccggcttcgg cgtccttctc gcggagctgg acgatgtcga cgcccgcggc
     8041 gaggacggct tcgaggaagg ggacgatgtc cgggcgacgc ccgccggtga cgaggtacag
     8101 cctcgccgcg gcgagccggt cgcgccgctt ggccggcttc atcccccggc gaccgcgcgg
     8161 acgacctcga ggcgatcgcc ctcggcgagc tcgacctctt cgtagcgcgc gcgctccagc
     8221 gcctccccgt tccgctcgac gatcacgacg cgcgggtcga ggccgagcga gcggacgaag
     8281 ccggcgaccg tcgtctccga cacctcatac tcgcggccgt tcgccacgat ccgcggcatc
     8341 tcagcccacc gccgcggtgc cgcggagcgc gcgggccatc ccctccggcg tcgaggggtc
     8401 gccccacacc gggtcgtagc ccatcgcccg caacgccgcc gcgacctcag ccggcgaacg
     8461 ttcgtcggcg atctcgaact gctcctcggc ctcgccgggg tgctcgtagc cgcctggctc
     8521 ggtgtgcgag ccggcgctcg tgtgcgtcac gccgatccga tggaggccgt cccgcagccc
     8581 cgccggctcc ctcgtcgaga gcacgatgcc agcgtcgggc atgcccaggc gcatcgcgca
     8641 gacgatctgc gcgaggtcgc ggtccccgac ctcgaccctg ggctggaacc cgctcgccga
     8701 cggacggatc cgcgggaccg agaccgtcac ctgggcccgc cagtacatgc gcatcaggaa
     8761 gcgcgcgtgc gccgccgtca cgatcgcctc ggcgcgccag tcgtcggcga ggccgtacag
     8821 cgcgccgagg cgatcgtgac ggc
//
LOCUS       DATLGG010000222         6310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_119647, whole genome shotgun sequence.
ACCESSION   DATLGG010000222 DATLGG010000000
VERSION     DATLGG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119647"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VM638_06310"
     CDS             complement(<1..653)
                     /locus_tag="VM638_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12070.1"
                     /translation="MKHRSIARFGRGLTLLALVLTTAGWGAPTPTTFVKPGSAEPGPD
                     LPGEVKLVNLSHVFAHDPGGARAKGTDIEFYTSTVPVRDENGEIVRDELGEPVLVEKD
                     FAVMGSYNRGAFVYDITDPENASLVRLIACNQTQNDVQIKSFPDAEGNDRWILALARD
                     GSDLPCTGISTSLMPSKGNSSGGGIAVFDITDPWDPKPVYRFRVPSGAHNFTFHPTKP
                     "
     gene            818..2536
                     /locus_tag="VM638_06315"
     CDS             818..2536
                     /locus_tag="VM638_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVM12071.1"
                     /translation="MRTSRMIVVVALSVALTGAVPAGANAPAPRPVDVGRLATFYRPT
                     PDLADLNNWRTYNAYNVVHYVSKNLPNRQPNDQTSTDPVGPGNLAACLALSNESQGCA
                     NHQLEYLSWFETAFGDVLKDFGVTFRTYQFNSGGSGPGTLASNAGRALNKLAIVPGAD
                     DPEDIVLIGSHYDQVDGSPFAAWDQTSGTGVMLRTAKILSDYWKSTGTRPSKTYVFAA
                     WDAEEAGALGSKFYVGTRSNRSSENGTLPKDPNVTLTAYLNHDPCGAAYPALYRGVPA
                     SRQPLSEKTGFIPVNVALHAPGGNTTERARMTAFNASIRPLINDLFNQIDDTLPVAPG
                     HAAAEVLPVFVSKEEAAALGHEALEQESVLKVNEAGSLLGFTTDAESFHDWVPTLNPF
                     PDVLGPHGVSTNPESTGWSADALVWVHTPLDSFEGLVAYTSPDQTGLTYSKGLAMSWE
                     LCSMLSAAVMLQPAQGGATPANTDPVAFFETPTPNRNGGTHVFNASGSYRYENATTRK
                     LDPSLVYEWDFGDGTTAEGRTVSHTYGTYGGGAAGRAYEVTLTVTDPQTQKSDRMMMK
                     IGTGRL"
     gene            complement(2533..3525)
                     /gene="speB"
                     /locus_tag="VM638_06320"
     CDS             complement(2533..3525)
                     /gene="speB"
                     /locus_tag="VM638_06320"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HVM12072.1"
                     /translation="MRVNRPSEPSYAGGATFSKLPLVLDPQELRGADVAILGAPIDET
                     TSHRPGARFGPRAIRLADVAGGSPPGWPHLDLGVDAASVLRIVDHGDAEVRPADAEWS
                     HAALRRAVRDVVSAGCVPVVLGGDHSIAHPNVSAMAEHFGAGTVGIIHFDAHADDAAE
                     VYGVERSHGTPMRLLVEEGSVRGDRIVQVGLRGYWPDPEDFDWAREQGFVWYTMAGME
                     ELGLSRVLDEVIERASAWERVWLSIDVDVADPAYAPGTGTPEPGGLTARELLGAVRRL
                     ALEVGFDGMEVVEVCPPYDHADITALLAHRVVLEALSGLAVRRSGGSPRPERPR"
     gene            3549..4349
                     /locus_tag="VM638_06325"
     CDS             3549..4349
                     /locus_tag="VM638_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVM12073.1"
                     /translation="MDDVVTFVDSGGVKLAVEVQGEGEPVTVLAHGLSGSRADVALFA
                     PFLPGTKVMFDFRGHGDSDRPGDGAYALDLFAQDVDAVATTFGATAFAGISLGTAAAL
                     RLLARRPDRFARLVFLLPARLDRSMSAHAQLMSLADLLERQGVETVAERVLEEEDRAG
                     RFTDFPAGRDFRRGVVLRMNREGVPRAIREVLAEGPLQDEGDLLARIEAPALVIGQRG
                     DPIHAAAVAEDLAETLPNAELLLFDDQHALMRRIPEVVGRVARFLAGT"
     gene            complement(4346..4705)
                     /locus_tag="VM638_06330"
     CDS             complement(4346..4705)
                     /locus_tag="VM638_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVM12074.1"
                     /translation="MSTRPVRPVGAGQDTSHKAVRMMADRVLVRRHDDGSERRSKAGL
                     VIPPTASSVSKRGVWVEVASVGPHVRHVREGDEVLLLPEEGIEVEIRGEEFLLVRERE
                     IHAVASEDEDAASGIYL"
     gene            4728..5597
                     /locus_tag="VM638_06335"
     CDS             4728..5597
                     /locus_tag="VM638_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HVM12075.1"
                     /translation="MPTVKDVLRDGTRRLVGTPAVDGMTSGRARWEAQVLLAHVLGHE
                     DPDDLDPEAGVGAPRERRFRDLIRRRAGGEPIPHIVGAMDFRGLRLEIRPGAFVPRQS
                     SEGLVELLVRRLRRRRRPVHVDLATGVGPVALAVAHEVPGAEVHGTDLLATGLRQARA
                     NARRLGLANVSFHRGDLYAPLPPDLRRRVDAVSIHPPYVPKDEVGDLPLEIRGYEPEE
                     VLTDHSEHGLGLVERAAEEGLAWLRPGGWMLMEVSPDRVRAVRGVLQRAGYRDVRSLK
                     AWPEVTRVVAGRA"
     gene            5623..>6310
                     /locus_tag="VM638_06340"
     CDS             5623..>6310
                     /locus_tag="VM638_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVM12076.1"
                     /translation="MHPIRGLLIDFGGVLTSSLGEAFERFCEEEGIPPDRLRDVLRAA
                     YDDEDPDHFVSLIETGRIEIDEFERLLAEVLSDGLQRPIRADGLVRRMVEDMEIDPEM
                     VDLVRRARAAGIRTALVSNSWGLHYYPMDLLEEIFDAILISGEVGLRKPGPEIFRAAA
                     ERLGLDVAGCVFVDDFGGNVAAAERLGMRGYLHEESARSVKELETLLGLPPAAAESDL
                     RMPRGAWARRH"
BASE COUNT          910 a         2274 c         2214 g          912 t
ORIGIN      
        1 ggcttggtcg ggtggaaggt gaagttgtgc gctccgctcg gaacgcggaa gcggtacacg
       61 ggcttcgggt cccacggatc ggtgatgtcg aagaccgcga tgccgccccc ggaggagttc
      121 cccttcgacg gcatcaggct cgtgctgatc ccggtgcagg ggagatcgct gccgtccctc
      181 gccagcgcga ggatccaacg gtcgttgccc tcggcgtccg ggaagctctt gatctggacg
      241 tcgttctggg tctggttgca cgcgatcagc ctgacgagcg acgcgttctc cgggtccgtg
      301 atgtcgtaga cgaaggcgcc tcggttgtag gaccccatca cggcgaaatc cttctcgacg
      361 aggacgggct cacccagctc gtcgcggacg atctcaccgt tctcgtcgcg gaccgggacc
      421 gtgctcgtat agaactcgat gtcggttccc ttggcgcgcg cgccgcccgg gtcgtgggcg
      481 aacacgtggc tcaggttgac gagcttcacc tcaccgggaa ggtccggtcc gggctcggcg
      541 ctcccgggct tcacgaacgt cgtcggggtg ggggcgcccc agccagccgt cgtgagcacc
      601 agcgccagca gcgtcaggcc tcgtccgaac cgtgcgatcg accgatgctt cacctgcgat
      661 ccctccttcg ggacgtgcgt cgttcctcag ccttctccga gtggtttcgc cgacggtctc
      721 cgccaaccct ttgtgcatct gcacaaaggg ttcgacctcc gagcgggcga acaaggctgg
      781 tagcggcgcg atggcgccgg cggagagcgg agacggcatg aggacgagcc ggatgatcgt
      841 cgtggtggca ctgagcgtcg ccctcacggg ggcggtgccg gccggcgcga acgcccccgc
      901 tcctcgaccg gtcgacgtgg gcaggctcgc aaccttctac cgtccgaccc ccgacctcgc
      961 cgatctcaac aactggcgga cctacaacgc gtacaacgtc gtgcactacg tctcgaagaa
     1021 cctcccgaac cgccagccga acgaccagac gtcgaccgac cccgtggggc ccgggaacct
     1081 ggcagcctgc ctcgcgctca gcaacgagag ccagggctgc gcgaaccatc agctcgagta
     1141 cctgagctgg ttcgagacgg ccttcggcga tgtcctgaag gacttcggcg tgacgttccg
     1201 gacctaccag ttcaactcgg gaggctcggg accgggcacc ctcgcgagca acgccgggcg
     1261 cgccttgaac aagctcgcga tcgtccccgg cgccgacgac cccgaggaca tcgtcctgat
     1321 cggcagtcac tacgaccagg tggacgggtc acccttcgcc gcgtgggatc agacctccgg
     1381 caccggcgtc atgttgcgga ccgcgaagat cctctccgac tactggaagt cgaccggcac
     1441 gcgtccgtcg aagacgtacg tgttcgcggc gtgggacgcc gaggaggcgg gggcgctcgg
     1501 gtcgaagttc tacgtcggaa cgaggagcaa ccgcagctcc gagaacggga cgctcccgaa
     1561 ggatccgaac gtcacgctga ccgcctacct gaaccacgac ccgtgcggcg ccgcctaccc
     1621 agcgctgtac cgcggcgttc ccgcctctcg ccagccgctg tcggagaaga caggcttcat
     1681 accggtgaac gtcgccctcc acgctcccgg cgggaacacg accgaacgag cccggatgac
     1741 ggcgttcaac gcgagcatcc ggccgctgat caacgacctg ttcaaccaga tcgacgacac
     1801 cttgccggtc gcgccggggc atgcggccgc cgaggtcctg ccggtgttcg tctcgaagga
     1861 ggaggcagcg gcgctcgggc atgaggcgct cgagcaggag agcgtgttga aggtgaacga
     1921 ggcgggctcc ctactcggct tcaccaccga tgcggagagc ttccacgact gggtcccgac
     1981 gctgaacccg ttccccgacg tcctcggtcc gcacggcgta tccaccaacc ccgagtccac
     2041 gggttggagc gccgacgcgc tggtctgggt ccacaccccg ctcgacagct tcgaagggct
     2101 ggtggcctac acgagccccg accagaccgg cctcacctac tcgaagggcc tcgcgatgtc
     2161 gtgggagctc tgctcgatgc tgtccgcggc ggtcatgctg cagcccgcgc agggaggggc
     2221 gacgccggcg aacacggacc ccgtcgcctt cttcgagact ccgacaccga accgcaacgg
     2281 cggcacgcac gtcttcaacg catcgggctc gtaccgctac gagaacgcca cgacgcggaa
     2341 gctggatccg tccctcgtct acgagtggga cttcggcgac ggcacgaccg ccgagggcag
     2401 gaccgtctcg cacacctacg ggacctacgg aggcggggcc gccggccgtg cctacgaggt
     2461 cacgctcacg gtcaccgatc cgcagacgca gaagtcggac cggatgatga tgaagatcgg
     2521 gacgggcagg ctctagcgag gccgctccgg cctcggcgag ccgccgctgc gccgcaccgc
     2581 caggccgctc aacgcttcga ggacgacgcg gtgggccagc agggccgtga tgtcggcgtg
     2641 gtcgtacggc gggcacacct cgacgacctc catcccgtcg aaccccacct cgagggcgag
     2701 ccgccgcacc gcgccgagca gctcgcgcgc cgtcagcccg ccgggctcgg gcgtgccggt
     2761 ccccggcgcg tacgcgggat cggccacatc gacgtcgatc gacagccaga cccgctccca
     2821 cgccgaggct cgctcgatca cctcgtcgag gacgcgcgac agccccagct cctccatccc
     2881 ggccatcgtg taccagacga agccctgctc ccgcgcccag tcgaaatcct ccgggtccgg
     2941 ccagtagccg cgcagcccga cctgcacgat ccggtccccc cggaccgagc cctcctcgac
     3001 gagcaggcgc atgggcgtcc cgtgcgagcg ctcgaccccg tagacctcgg ccgcgtcgtc
     3061 ggcgtgggcg tcgaagtgga tgatgccgac ggtccccgcg ccgaaatgct cggccatcgc
     3121 cgagacgttc ggatgagcga tcgagtggtc gccgccgagg acgaccggga cgcacccggc
     3181 cgagacgacg tcgcgcaccg cccgccggag ggccgcgtgg ctccactcgg cgtcggccgg
     3241 tcgaacctcg gcgtcgccgt ggtcgacgat ccggagcacg ctggccgcgt cgacgcccag
     3301 gtcgaggtgc ggccatcccg ggggcgagcc accggccacg tccgcgaggc ggatcgcccg
     3361 tgggccgaac cgggcgcccg gccggtgcga ggtcgtctcg tcgatcggcg cgccgaggat
     3421 cgcgacgtcg gccccacgca gctcttgggg gtcgaggacc agggggagct tcgagaaggt
     3481 cgcgccgccc gcgtaggagg gctcgctggg ccgattcaca cgcatgaccc tgagtatcct
     3541 ctgtcggcgt ggacgacgtc gtgaccttcg tcgactccgg gggcgtcaag ctcgccgtcg
     3601 aggtccaggg tgagggcgaa cccgtcaccg tcctcgcgca cggcctgagc ggcagccgcg
     3661 cggacgtcgc cctcttcgcg ccgttcctcc cggggacgaa ggtgatgttc gacttccgcg
     3721 gtcacggtga cagcgaccgg cccggcgacg gcgcctacgc gctcgacctg ttcgcgcagg
     3781 acgtcgacgc ggtcgcgacc acgttcggcg cgacggcgtt cgccgggatc tcgctcggta
     3841 cggccgccgc gctccgtctc ctggcccgtc gcccggaccg gttcgcgcgc ctcgtgttcc
     3901 tccttccggc gcgcctcgac cggtcgatga gcgcgcacgc gcagctcatg tcgctcgcgg
     3961 atctcctcga gcggcagggg gtggagaccg tcgccgagcg cgtcctcgag gaggaggatc
     4021 gcgccgggcg cttcaccgac ttccccgcgg gccgcgactt ccggcgtggg gtcgtcctgc
     4081 ggatgaaccg cgagggcgtc ccccgcgcga tccgggaggt gctggccgaa ggaccgctcc
     4141 aggacgaggg ggatctgctg gctcgcatcg aggcaccggc gctcgtgatc ggacagcggg
     4201 gggatccgat ccacgccgcg gcggtcgccg aggacctcgc cgagaccctg ccgaacgcgg
     4261 agctcctgct gttcgacgac cagcacgccc tgatgcgccg gatcccggag gtcgtcggga
     4321 gggtcgcgag gttcctggcc gggacctaga ggtagatccc cgaggccgcg tcctcgtcct
     4381 ccgaggcgac cgcatggatc tcgcgctcgc ggacgagcag gaactcctca ccgcggatct
     4441 cgacctcgat cccttcctcg ggcaggagca gcacctcgtc gccctcccgc acgtgccgca
     4501 cgtggggccc gacggaggcg acctcgaccc acaccccgcg cttgctcacg ctgctcgccg
     4561 tcggcgggat cacgagcccc gccttcgagc gtcgctcgct gccgtcgtcg tggcgacgga
     4621 cgaggacgcg gtcggccatc atccgcaccg ccttgtgcga cgtgtcctgc cccgccccaa
     4681 ccgggcggac cggtctggtg ctcatccgtg tggaaggata cgacgacatg ccgacggtga
     4741 aggacgttct ccgggacggg acgcggcggc tggtggggac gccggccgtg gacgggatga
     4801 cgtcgggccg cgcacggtgg gaggcgcagg tgctgctggc gcacgtcctc ggacacgagg
     4861 acccggacga cctcgatccc gaggcgggcg tcggcgcgcc gcgcgagcga cgcttccgcg
     4921 acctgatccg ccgtcgggct gggggtgagc cgatcccgca catcgtcggg gcgatggatt
     4981 tccgcggcct gcggctcgag atccgccccg gcgcgttcgt cccccgacag tcgagcgagg
     5041 gactcgtcga gctcctcgtc cgtcgcctcc gccgccgccg ccggccggtc cacgtcgacc
     5101 tcgcgaccgg cgtgggcccg gttgccctgg cggtggcgca cgaggtcccg ggtgccgagg
     5161 tccacgggac cgatctgctc gcgaccgggc tccggcaggc ccgtgcgaac gcccggcggc
     5221 tggggctcgc gaacgtctcg ttccatcgcg gcgacctcta cgcgcccctc ccgcccgacc
     5281 tgcggcgacg cgtcgacgcc gtctcgatcc acccgccgta cgtcccgaag gacgaggtcg
     5341 gcgacctccc cctcgagatc cgcggctacg agcccgagga ggtgctgacc gaccactcgg
     5401 agcacgggct cggcctggtc gagcgcgcgg cggaggaggg gctcgcgtgg ctccgccccg
     5461 gtggatggat gctgatggag gtgagccccg accgcgtgcg tgcggtgcgg ggggtgctcc
     5521 agcgcgccgg ctaccgggac gtccgcagcc tcaaggcatg gcccgaggtg acgcgggtcg
     5581 tcgccggccg ggcgtgacgg tgccagcggg taccctggga ccgtgcaccc gatccgcggc
     5641 ctgctgatcg acttcggcgg cgtcctgacg tcgtcgctcg gcgaggcgtt cgagcggttc
     5701 tgcgaggagg aggggatccc gccggaccgt ctccgcgacg tgctccgtgc ggcgtacgac
     5761 gacgaggatc cggatcactt cgtctccctg atcgagacgg gccgcatcga gatcgacgag
     5821 ttcgagcgcc tgctcgccga ggtgctctcc gacggtctcc agcggccgat ccgcgccgat
     5881 ggcctcgtgc ggcggatggt ggaggacatg gagatcgacc ccgagatggt cgatctcgtg
     5941 cgccgcgcgc gcgcggccgg gatccggacg gcgctcgtct cgaactcctg gggcctgcac
     6001 tactacccga tggacctgct cgaggagatc ttcgacgcga tcctgatctc gggcgaggtc
     6061 gggctccgca agcccggccc ggagatcttc cgagccgccg ccgagcgcct cgggctcgac
     6121 gtggccggtt gcgtcttcgt cgacgacttc ggggggaacg tggctgccgc cgaacgcctc
     6181 gggatgcgcg ggtacctcca cgaggagagc gcccgctccg tgaaggagct ggagacgctg
     6241 ctcgggctcc cgccagccgc cgcggagagc gacctgcgga tgccgcgtgg cgcctgggcg
     6301 cgtcgtcacg
//
LOCUS       DATLGG010000223         5051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_196402, whole genome shotgun sequence.
ACCESSION   DATLGG010000223 DATLGG010000000
VERSION     DATLGG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196402"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VM638_06345"
     CDS             complement(<1..504)
                     /locus_tag="VM638_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVM12077.1"
                     /translation="MTDCSRVRPLLAEHALGILEPAERGAVGAHLAWCAGCRKEAREL
                     AEGAAVVAQGIVPAEPPETLGPRILGTARRTPRGRGRRAFVGALAAAVLAMGAFGWAV
                     AMTGRAEQLEAAARSAAERAERFEAVVAQLLLDAGEGRVLSARLEAVEGRSGGGRAVI
                     LDSPGGVD"
     gene            complement(501..1055)
                     /locus_tag="VM638_06350"
     CDS             complement(501..1055)
                     /locus_tag="VM638_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM12078.1"
                     /translation="MTGPLAPQDEQDLMRRLALGDEDAVRLVYERFGRAVYSLGLRML
                     RTPESAEDLTQEVFLTAWRRAGGYDPRRGRLSTWLMTIAHNAAVDRIRHDAARPRPAL
                     KAIDETVAPSLVEEDGVIDRLLAGHVLASLSHRERRLIELAYFRGWSAREIAEADGIP
                     LGTVKTRLRATLIRLRAVHKGEAS"
     gene            complement(1108..1779)
                     /locus_tag="VM638_06355"
     CDS             complement(1108..1779)
                     /locus_tag="VM638_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM12079.1"
                     /translation="MADTKALEIHKALADDTRFRLYRHLRLSGRPVSVQEMSRRLGLH
                     PNTLRPHLRRLEEAGLVSRQVRKVARVGRPQTQYQALEASAEEGRDFRLLAEMLCGLL
                     RTKREIERARGLAREWGAYLVSQGGPKPGAKLPPGRSLAVLQEAMAAAGFDPRFRRGR
                     GGVEVTMRDCPFRELAGDHRELVCTLHQGLVEGMAAGLKPSSALRDFRPFAERGICRM
                     RLGPA"
     gene            1822..2934
                     /locus_tag="VM638_06360"
     CDS             1822..2934
                     /locus_tag="VM638_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HVM12080.1"
                     /translation="MVTEQQVRDALRKVLDPEIGKPIEDLGMLKGIEIDGGTVRIDIL
                     LTIAGCPLKDRIEHDVSHALAPLEGIEHIEVRMGTMTGEQREGLVTSLRGQQPDTPTF
                     FADGRTEVIAVASGKGGVGKSSVTANLAAAMAAEGQRVGVLDADVWGFSIPRMLGVTG
                     QPVGFNEMILPLESHGVKVISMGFFVKDDTPVIWRGPMLHKALNQFLGDVYWGDLDYL
                     LIDLPPGTGDVSLSLATFLPSGSMVVVTTPQEAAHKVAERAGRMAQHPNVKMKLLGVI
                     ENMAGFVCPHCDEVTELFGSGGGARTAEALGVPLLGSVPMQVSLREGSDAGAPVVVTE
                     PDSPAGRAFREVASELTSRTSTRLGKALPLTVAPRG"
     gene            complement(2957..3436)
                     /locus_tag="VM638_06365"
     CDS             complement(2957..3436)
                     /locus_tag="VM638_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVM12081.1"
                     /translation="MTPKGGSRTPAASGARKDRSERFTAEERAAMKERADELRSERRG
                     GRGRKADGESEVLAKIAEMAQPDRAMAERLHALVKEAAPDLVPRTWYGMPAYAKDGKV
                     VCFFQGAQKFKSRYATLGFNDAANLDDGPMWPTAFALEELTPAVEARIRALVKQAAG"
     gene            complement(3433..4371)
                     /locus_tag="VM638_06370"
     CDS             complement(3433..4371)
                     /locus_tag="VM638_06370"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HVM12082.1"
                     /translation="MTLLAAIGWPVIERIGPVSPHGIGIAAGYLLGAMWMVREGRKRG
                     LDEDHVGTILVWSLVGAIIGARLFYVIGHFNEFDGIVDMLSIWRGGISLIGGIVGAMA
                     LAYPIMRRYGYRFLQVMDSAAIGLAFGIMVGRIGDLVIGDHLGKHTTFFLGFRYSGGD
                     LPGYTCLGERMFQECHTTLANGTQQVIRHGDVVHQTALYDMLIAGVLFALLFWINRRP
                     RREGVLIATFAIWYGAGRVLTDFLRVDKTWFGLTGSQWTSIAAILLSIGLLVRYAMRP
                     GPGPQPVLAGGEPGSVEGEPGPVEGEDQPPGVPGQG"
     gene            complement(4382..5029)
                     /locus_tag="VM638_06375"
     CDS             complement(4382..5029)
                     /locus_tag="VM638_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVM12083.1"
                     /translation="MTKRTTGPSAAFDPRSRRPGPVGTGLLVSAALGLAAATVRGRGR
                     VLDDRVFNLVNSEMEHPLLDRFFMGVTELGSLYASGAAGLTVLASGRRREALDALGAA
                     AATWLIGQWLKQAWRRNRPFQSDIPFRLLIGKPRGTSWPSSHPMVLLAFVTVAGRNLR
                     LGKPVRTGLNGLAGLVAASRVYLGVHYPADVAGGLLLGRAIADTWSRTVSPRAVG"
BASE COUNT          729 a         1875 c         1768 g          679 t
ORIGIN      
        1 gtcgaccccg cccggcgagt cgaggatgac cgcccggccc ccgccggagc gcccctcgac
       61 cgcctcgagc cgcgccgaga gcacgcgccc ctcgccggca tcgaggagga gctgcgcgac
      121 caccgcttcg aagcgctcgg ctcgctccgc cgcgctcctc gccgccgcct cgagctgctc
      181 ggcgcgtccc gtcatcgcga cggcccaccc gaaggcgccc atcgccagga ccgccgccgc
      241 gagcgctccg acgaaggcgc gccgcccacg gccccgaggg gtccggcgcg ccgtcccgag
      301 gatccgcggc ccgagagtct ccgggggctc cgccgggacg atcccctgcg cgacgacggc
      361 cgctccctcg gccagctcgc gcgcctcctt gcggcacccg gcgcaccaag cgaggtgcgc
      421 accgaccgcg ccgcgctcgg ccggctcgag gatcccgagt gcgtgctcgg cgaggagcgg
      481 ccgcaccctc gagcagtccg tcacgaggcc tcccccttgt ggaccgcgcg gagccggatc
      541 agcgtcgccc ggagccgggt cttgacggtc ccgagcggga tcccgtccgc ctcggcgatc
      601 tcgcgcgcgc tccatcctcg gaagtaggcg agctcgatca gccgtcgctc gcggtgcgag
      661 agggaggcga ggacgtgacc ggccaggagc cggtcgatga ccccgtcctc ctcgacgaga
      721 ctgggggcca cggtctcgtc gatcgccttg agggccggcc gcggtcgcgc ggcgtcgtgc
      781 cggatccggt cgacggcggc gttgtgagcg atcgtcatga gccaggtcga cagccggccg
      841 cgccgggggt cgtagcctcc cgcgcgccgc caggccgtca ggaacacctc ctgcgtcagg
      901 tcctccgcgc tctcgggcgt ccgcagcatc cgcagaccga gcgagtacac ggcgcggccg
      961 aagcgctcgt agacgaggcg caccgcgtcc tcgtcgccga gcgcgagccg tcgcatcagg
     1021 tcctgctcgt cctgcggcgc caggggtccg gtcatggacc gccctccatg accgttctac
     1081 ggctgcgccg gccgatcggt ttcccgctca tgccggcccc agccgcatcc ggcagatgcc
     1141 tcgttccgcg aacgggcgga agtcccggag agccgagctg ggcttcagcc ccgccgccat
     1201 cccctccacg agaccctggt ggagcgtgca gacgagctcg cggtggtcgc cggcgagctc
     1261 gcggaagggg cagtcccgca tcgtcacctc gacgccgcca cggccacggc ggaagcgcgg
     1321 atcgaacccc gccgcggcca tcgcctcctg caggaccgcg aggctccgcc cggggggcag
     1381 cttcgccccg ggcttcggcc cgccctgcga gacgaggtag gcaccccact cccgtgcgag
     1441 cccgcgagcc cgctcgatct cccgcttcgt gcggagcagg ccgcagagca tctccgcgag
     1501 caggcggaag tcgcgcccct cctcggcgct cgcctcgagc gcctggtact gggtctgcgg
     1561 ccggccgacc cgcgccacct tccggacctg gcgcgagacg aggcccgcct cctcgagacg
     1621 tcgcaggtgc ggccgcagcg tgttcgggtg gagaccgagg cgccgcgaca tctcctggac
     1681 cgaaacgggg cgccccgaca ggcgcaggtg ccggtacagg cggaaccgcg tgtcgtccgc
     1741 gagcgcttta tggatctcga gggccttcgt atccgccatc agcgtctagt atggaggccg
     1801 aagccccacc ggaggtcccc gatggtcacc gaacagcagg tccgtgacgc cctgaggaag
     1861 gtgctcgacc ccgagatcgg gaagccgatc gaggatctcg gcatgctgaa gggcatcgag
     1921 atcgacgggg gcaccgtccg gatcgacatc ctcctgacga tcgcgggatg cccgctcaag
     1981 gaccgcatcg agcacgacgt ctctcacgcg ctggccccgc tcgaggggat cgagcacatc
     2041 gaggtgcgga tgggcacgat gaccggcgag cagcgagagg gcttggtgac ctcgctgcgc
     2101 ggtcagcagc ccgacacccc gaccttcttc gccgacggcc ggaccgaggt catcgcggtg
     2161 gcgtcgggca agggcggcgt cggcaagtcc agcgtgacgg cgaacctcgc cgcggcgatg
     2221 gccgccgagg gccagcgggt cggcgtcctc gacgccgacg tctgggggtt ctcgatcccc
     2281 cgcatgctgg gcgtcaccgg tcagcccgtc gggttcaacg agatgatcct ccccctcgag
     2341 agccacggcg tgaaggtcat ctcgatgggc ttcttcgtga aggacgacac gccggtgatc
     2401 tggcgcggcc cgatgctcca caaggcgctg aaccagttcc tcggcgacgt gtactggggc
     2461 gacctcgact acctgctgat cgacctgccc ccggggaccg gcgacgtctc cctctccctc
     2521 gcgacgttcc tcccgagcgg ctccatggtc gtcgtgacga ccccgcagga ggcggcgcac
     2581 aaggtggccg agcgcgccgg gcggatggcg cagcacccga acgtgaagat gaagctgctc
     2641 ggcgtcatcg agaacatggc cgggttcgtc tgtccgcact gtgacgaggt gacggagctc
     2701 ttcgggagcg gcggcggtgc acggaccgcg gaggcgctcg gggttccgct cctcgggtcg
     2761 gtcccgatgc aggtctcgct gcgtgaggga tccgacgccg gcgcacccgt ggtcgtgacc
     2821 gagccggact cccccgccgg tcgcgccttc cgcgaggtcg catccgagct cacgtcgcga
     2881 acctcgaccc ggctcggcaa ggccctgccg ctgaccgtcg cgccccgcgg ctgacgaacg
     2941 cgcgctcgat ccggcctcag cccgccgctt gcttcacgag cgccctgatc cgcgcctcga
     3001 cggccggggt cagctcctcc aacgcgaacg ccgtcggcca catcggtccg tcgtcgaggt
     3061 tcgcggcgtc gttgaagccg agcgtggcgt agcgcgactt gaacttctga gcgccctgga
     3121 agaagcacac gaccttgccg tccttcgcgt acgccggcat gccgtaccag gtcctcggca
     3181 cgaggtcggg cgcggcctct ttcacgagcg cgtggagccg ctcggccatc gcgcggtcgg
     3241 gctgggccat ctcggcgatc ttcgcgagga cctcgctttc gccatccgcc ttcctccctc
     3301 ggcccccgcg ccgctccgac ctcagctcgt cggcgcgctc cttcatcgcg gcgcgctcct
     3361 cggccgtgaa acgttcggag cgatccttgc gagcgcccga ggcggcgggc gttctcgacc
     3421 cccccttggg ggtcatccct ggcccggtac gccgggtggc tggtcctcgc cctcgacggg
     3481 gcccggctcg ccttcgacgg agcccggctc gccgccggcg agcaccggct gcgggcccgg
     3541 tcccggacgc atcgcgtagc ggacgagcaa cccgatcgag agcaggatcg ccgcgattga
     3601 ggtccactgg ctcccggtga gcccgaacca ggtcttgtcg acgcggagga agtcggtgag
     3661 gacgcgcccg gcgccgtacc agatcgcgaa cgtcgcgatg aggaccccct cgcggcgcgg
     3721 gcgccggttg atccagaaca gcagcgcgaa cagcacgccg gcgatcaaca tgtcgtagag
     3781 cgccgtctgg tggacgacat caccgtggcg gatcacctgc tgggtcccgt tggcgagcgt
     3841 cgtgtggcac tcctggaaca tccgctcgcc gaggcaggtg tagccgggga ggtcgccgcc
     3901 cgagtagcgg aacccgagga agaacgtcgt gtgcttgccg aggtggtccc cgatgacgag
     3961 gtcgccgatg cgtccgacca tgatgccgaa ggccaggccg atcgccgccg agtccatcac
     4021 ctgcaggaag cggtagccgt agcggcgcat gatcggatag gcgagcgcca tcgcgccgac
     4081 gatgcccccg atcaggctga tcccgccgcg ccagatcgac agcatgtcga cgatcccgtc
     4141 gaactcgttg aagtggccga tcacgtagaa gaggcgcgcg ccgatgatcg cgccgacgag
     4201 cgaccagacg aggatcgtcc cgacgtgatc ctcgtccaga ccgcgcttgc gtccctcccg
     4261 caccatccac atcgcgccga ggagatagcc cgcggcgatg ccgatgccgt gcggggagac
     4321 cggcccgatc cgctcgatga ccggccagcc gatcgctgcg aggagggtca cgcgcgggag
     4381 tctatccgac ggcccggggc gagacggtgc gcgaccaggt gtcggcgatc gcccgcccga
     4441 gcagcaggcc cccggcgacg tcggccgggt agtgcacgcc gagatagacc cgcgacgcgg
     4501 cgaccagccc ggcgaggccg ttcagacccg tccgcaccgg cttccccagc cgcaggttcc
     4561 gccccgcgac ggtgacgaag gcgagaagga ccatcgggtg gctgctcggc cacgaggtgc
     4621 cgcgcggttt cccgatcagg agccggaagg ggatgtcgga ctggaacggc cggttccgcc
     4681 gccaggcctg cttcaaccac tgtccgatca gccaagtcgc ggccgccgcc ccgagcgcat
     4741 cgagggcctc gcggcgacgt cccgaggcca gcaccgtgag cccggctgcg cccgaagcgt
     4801 agagggatcc gagctccgtg acgcccatga agaagcggtc cagcagcggg tgctccatct
     4861 cggagttcac gaggttgaac acccggtcgt cgaggacgcg gccgcggccg cgcacggttg
     4921 ccgcggcaag accgagcgcg gccgacacca gcaggccggt gccgacgggg cccggccggc
     4981 gcgatcgcgg atcgaacgcc gccgacggac cggtggttct cttcgtcacg gtcgggcatc
     5041 atacccacac g
//
LOCUS       DATLGG010000224         7017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_73787, whole genome shotgun sequence.
ACCESSION   DATLGG010000224 DATLGG010000000
VERSION     DATLGG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73787"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VM638_06380"
     CDS             complement(<1..864)
                     /locus_tag="VM638_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HVM12084.1"
                     /translation="MELARTLAGRVDDALDRMEAATAEALDALGAFDEAVEYRSDGHG
                     ACAEWLCARRAMSWATASELVRLARALRRLPEIAGAYRRGELCWDQLRPLCQVALAET
                     DRAWAERAPRMSPAELWREVERQRQVRAERAADDRKARRLWMRWDPERRFLQVAGDLP
                     GEQGVAFQAAVRRRAKEIPRDPEAFSPAEARQADAVVELVSASGGRPAPATVVVHADA
                     KLLADAPPDGTRRLAETAEGLRLAAEAVRRLACDAQVELHLEREGHVVGIGRAGRAVP
                     GWLLRAIEHRDA"
     gene            1055..1408
                     /locus_tag="VM638_06385"
     CDS             1055..1408
                     /locus_tag="VM638_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12085.1"
                     /translation="METLGILINAAVVAAVGLLLAWQTRGRFDAQNQRFDTRFQAIDT
                     RFDAVDQRFGGIDQRLDRLDHRIDRLEDRLDKRIDGLETRLGRLETETVGIRTDLTRM
                     AFALRIAPETQTGND"
     gene            1483..4686
                     /locus_tag="VM638_06390"
     CDS             1483..4686
                     /locus_tag="VM638_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HVM12086.1"
                     /translation="MRRSRHAPRTHALLLATAFVALGHVPAGAGSLSTTHGDLRGVVS
                     HPRWGPIAGIRVSVTSNHAEPIQTSTCTYLNGSYLLTLPAGSYTVRFDDDDASCPQPP
                     GLFRTQWWQAAETEEAATRLEIVAGQQHTADASLQLADESTPFISGVLSGPPGQSPHS
                     VRVEAFDADDAADASPVASTCTPSEGPAYFRLMVPASADYVVRFAGHDTDPCPGGLPV
                     FISEWYDDSSTRTGARTIAGTSLPFGPLEATLSPGRQITGTVEDQQRRDGIDGACVDI
                     FSIRPPDRPQDIFDWRSPHDPIVTVRTAANGAYASPVMARGDEYAVRFRGPDGCGGTG
                     AYVDDWYGAGIGTPYRTVDVSLSQGWKSTGISGSLYPCPEADCSAVKDLRIEAIVENG
                     PVFTPMTYRVRVENRGPGTETGVTLTTLLDERLQFSAASAPGGCERSGRTVVCALGSL
                     ERWETANARITVIPNEARTYLTQATASGDGFDPFPADNQRLFSDAVIASRNADTSLSA
                     SGSADPVTVNGWFRYDFRISNAGPAAAEGVLAVANLAPGLRIGPEALPEGAPTYTASQ
                     GVCDLDPTRNRIVCQLGKLERNGSALVSIPVRAPGTPGNLESGFTVRSSNLDPEPGNN
                     QAVGQTLVVPVVASQPTLTGASRDPATGELTAVASKTDPQPIAISALVACPNGSAPTS
                     VTLTIGSLTTPMTPEGEGRYGATVTAEDLQAGAVGDVIITAACAGGNVANQVGTIVLY
                     DPSGIVTDAVTDQPVNGATVTLHKVADWTPWSEGEEQPAKTCQTVGTRPGGAEGSWTQ
                     TAPTYAGEVVDPASPEIDPAENPQITGAGGRLPGQYAWDVAEGCWYVVVEKTGYLTLT
                     SPVVGVPPAVTDLHLELTPVPGFRTTTLSSSFADRTYEEPVTLSGAVSSATDGCAAAS
                     VRIWRDVLGDQVDFQVYRETTAGPSGAFSVPVTPDASATYRAEAVAEGACLAAESATQ
                     TVRVAAKVGLKASSTRVSKGTTVKLTATVAPCPGHPGHAVTLVRGTKQIGEKQLGSTC
                     KAVFKVKMLKTRTFEARYEQQHDDHDAGISKRVTVRV"
     gene            complement(4792..4920)
                     /locus_tag="VM638_06395"
                     /pseudo
     CDS             complement(4792..4920)
                     /locus_tag="VM638_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
     gene            complement(4987..5436)
                     /locus_tag="VM638_06400"
     CDS             complement(4987..5436)
                     /locus_tag="VM638_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVM12087.1"
                     /translation="MPSEADIRERLGWAASSEEHEKIRRLWIEHSKAEDARDLEGLIA
                     TLTPDCVYELVWSGERWKGHDGARRFYLELLTAFPDIDFDLQDIVIGPQGVFEVANVT
                     GTHHGPWAGVEPDGRQKRFQVVIHFPWDPQAELFAGEKVYLGPIESA"
     gene            5510..6346
                     /locus_tag="VM638_06405"
     CDS             5510..6346
                     /locus_tag="VM638_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVM12088.1"
                     /translation="MRRRPPRSSAPAAATPDWRSYDSVAETYERVRAPLHADPAADLV
                     RLMELPPGGRVLDVGTGPGVAAELAGEELGAEGLVVGVDPSIGMLRTAIRRVPHVIQA
                     QALDLPFRDGVFDGVIATFVVFFFRRYETGLAELSRVLRPGAELGLTTWGGGDDAFAA
                     TWREIAEAYVGRDLLRDATRQAAPWEEHFSDPGRVKETLRDAGFRNVRVEVRTYRHTV
                     SLEDYIAGRETSVTGRFLRGMLGEALWARFHARVTEEFRNRFPDPIGDSNDVLLTLAR
                     KP"
     gene            complement(6343..>7017)
                     /locus_tag="VM638_06410"
     CDS             complement(6343..>7017)
                     /locus_tag="VM638_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HVM12089.1"
                     /translation="DAKGMEWMKCDMAGAASILGTMQAVARLKPPVNVVAAIPSAENM
                     PGGSAIRPGDVLRHRGGKTSEVLNTDAEGRLILADALAYLTEKKPSVIVDTATLTGAC
                     VIALGEELWGVMGSDRGLVRELLDAGEAAGEPGWELPLWDGYKRHIESQIAEVKNIGA
                     RGAGTITAALFLKHFVGDVPWAHLDIAGTAYSERPGDYWPRGATGSPVRTLVRFIEGR
                     ANGKRR"
BASE COUNT         1005 a         2645 c         2429 g          938 t
ORIGIN      
        1 cgcgtcccga tgctcgatcg cccgaagcag ccagcccggg accgcccgcc cggcgcggcc
       61 gatccccacg acgtgcccct cccgctcgag gtgcagctcc acctgcgcgt cgcaggcgag
      121 tcggcggacc gcctcggccg ctagccggag cccctcggcg gtctccgcca ggcgccgggt
      181 gccgtcgggg ggagcatccg caaggagctt cgcatcggcg tgcacgacca cggtcgcggg
      241 ggcgggcctg ccccccgagg cactcacgag ctccaccacc gcatccgcct gccgcgcctc
      301 cgcgggggag aaggcctcgg ggtcgcgggg gatctccttc gcccggcgcc gcacggcagc
      361 ctggaacgcc accccctgct cgccgggaag gtcgcctgcg acctgcagga accgccgctc
      421 ggggtcccag cgcatccaca gcctgcgagc cttccggtcg tcggcggccc gctcggcgcg
      481 cacctggcgc tgccgctcca cctcccgcca gagctccgcc ggggacatcc gcggggcccg
      541 ctcggcccag gcacgatcgg tctctgccag cgcgacctgg cacagcggcc ggagctggtc
      601 ccagcagagc tcgccccggc ggtacgcccc ggcgatctcc gggagccgcc ggagcgcccg
      661 ggcgaggcgc acgagctccg aggctgtggc ccagctcatc gcccgccgcg cacacagcca
      721 ctcggcgcaa gcgccgtgac catccgagcg gtactccacc gcctcgtcga aggcccccag
      781 cgcatccagc gcctcggccg tggccgcctc catccgatcg agcgcgtcgt ccacgcggcc
      841 ggcaagcgtg cgggcgagtt ccatcgatgt gatcctaccg aggggcaccg acacgaacgt
      901 ccggccgacc ggccctcacg cgaggtacga ctccctcttc atgagcgccg ggtcgggcag
      961 cacgttcacg caggccggca ccccgcgcgc gaacccgtct gcccccggtg ctacgctggc
     1021 cttcgacgcc gatcctcccc cgggaaggac gacgatggag acgctcggca tcctgatcaa
     1081 cgccgccgtg gtggcggcgg tcggcctgct cctcgcatgg cagacgcgag gacgcttcga
     1141 cgcccagaac cagcgcttcg atacccggtt ccaggcgatc gacacgcggt tcgacgctgt
     1201 ggaccagcgg ttcggcggca tcgatcagcg gctcgatcgc ctcgaccatc ggatcgaccg
     1261 gttggaggac cggctcgaca agcggatcga cgggctggag acgcggctcg ggcgcctgga
     1321 gaccgagacc gtcggcatcc gcaccgacct cacccggatg gccttcgccc tccgcatcgc
     1381 ccccgagacg cagaccggga acgactgaca ccgtcgggcc tatcgcaaac catccgaacg
     1441 agctattccg agaaactccc gcaatgccta ccttcgagct gcatgcgacg ctcccgccac
     1501 gccccgagga cccatgcgct gctgctcgcc acggcgttcg tcgcgctcgg ccacgtcccc
     1561 gccggggccg ggtcgctctc caccacccac ggcgacctcc gcggggtcgt gtcccatccc
     1621 cgatggggcc cgatcgccgg catccgcgtg agcgtcacct cgaaccatgc cgagccgatc
     1681 cagacctcga cctgcaccta cctgaacggg tcgtacctgc tcacgctgcc cgccggctcc
     1741 tacaccgtcc ggttcgacga cgacgacgcg agctgcccac agcccccggg gctcttccgg
     1801 acgcagtggt ggcaggcagc cgagaccgag gaggctgcga cccggctcga gatcgtggca
     1861 ggccagcagc acaccgcgga cgcgtccctc cagctcgcgg acgagagcac cccgttcatc
     1921 tccggggtcc tgagcgggcc cccgggccag tcgccgcaca gcgtgcgcgt cgaggcgttc
     1981 gacgccgacg acgccgccga cgcgtcaccc gtagcgagca cctgcacgcc gtccgagggg
     2041 cccgcgtact tccggctgat ggttccggcc tccgccgact acgtcgtccg cttcgccggc
     2101 cacgacacag acccctgccc aggcgggctg ccggttttca tctcggagtg gtacgacgac
     2161 tcatccaccc ggacaggcgc gaggacgatc gcgggcacga gccttccgtt cgggccgctc
     2221 gaggccaccc tctcccccgg ccgccagatc accgggacgg tcgaggacca gcagcgccgg
     2281 gacgggatcg acggagcgtg cgtcgacatc ttcagcatca gaccgccgga tcggccccag
     2341 gacatcttcg actggaggag cccgcacgac ccgatcgtga ccgtccgcac ggcggcgaac
     2401 ggcgcctacg cgagcccggt catggctcgc ggcgacgagt acgccgtccg gttccgcggc
     2461 ccggacggat gcggcggaac cggcgcctac gtcgacgact ggtacggagc cgggatcggc
     2521 acgccctacc ggacggtcga cgtcagcctg agccagggct ggaagagcac cggcatcagc
     2581 gggtcgctct acccctgtcc ggaggcggac tgctccgcgg tgaaggatct gcggatcgag
     2641 gcgatcgtcg agaacggccc cgtcttcacg ccgatgacgt atcgggtccg ggtggagaac
     2701 cgcgggccgg gcaccgagac cggcgtcacg ctgacgacgc tgctcgacga gcgtcttcag
     2761 ttctccgccg cgagcgcgcc cggggggtgc gagcggtccg gacgaacggt cgtctgcgcc
     2821 ctcggctcgc tcgagcggtg ggagacggcg aacgcgcgga tcacggtcat cccgaacgag
     2881 gcgaggacgt acctgaccca ggcgaccgcg tcgggtgacg gcttcgatcc cttcccggct
     2941 gacaaccagc ggctgttctc ggacgccgtt atcgcgagcc ggaacgccga cacgagcctg
     3001 tcggcctcag ggtccgcgga cccagtgacc gtcaacgggt ggttccgcta cgatttccgc
     3061 atctccaacg ccggcccggc ggcggcggag ggcgtcctgg ccgtggcgaa cctcgcgcct
     3121 ggactccgga tcggccccga ggcgctgccc gagggggcgc cgacctacac cgcctctcag
     3181 ggggtgtgcg acctggatcc gacccgcaac cgcatcgtct gccagctcgg aaagctcgag
     3241 cgcaacgggt cggccctcgt ctcgatccct gtgagggccc cggggacgcc ggggaacctc
     3301 gagagcggat tcaccgtgcg gtcgtcgaac ctcgacccgg agcccgggaa caatcaggcc
     3361 gtggggcaga cgctcgtcgt gccggtcgtg gcatcccaac cgacgctgac cggcgcgagc
     3421 agggatcccg cgaccgggga gctgacggcg gtcgcgtcga agaccgaccc gcagccgatc
     3481 gcgatctcgg ccctggtcgc gtgcccgaac ggcagcgcgc cgacctcggt cacgctcacg
     3541 atcgggtcgc tcaccacgcc gatgacgccg gaaggggaag gccgctacgg cgccacggtc
     3601 acggcggagg acctccaggc gggagcggtg ggcgacgtga tcatcaccgc cgcctgcgcc
     3661 ggcgggaacg tggcgaacca ggtcgggacg atcgtcctct acgacccctc aggcatcgtc
     3721 accgacgcgg tcaccgacca gccggtgaac ggcgcgaccg tgaccctgca caaggtcgcc
     3781 gactggacgc cgtggagcga aggcgaggag cagccggcga agacgtgcca gaccgtcggc
     3841 acgcgcccgg gcggcgccga gggctcctgg acccagacgg cgcccaccta cgccggcgaa
     3901 gtggtggatc ccgcctctcc ggagatcgat cccgccgaga acccgcagat caccggcgcg
     3961 ggcggccggc tgccggggca gtacgcgtgg gacgtggccg agggctgctg gtacgtcgtc
     4021 gtcgagaaga ccggctacct gacgctgacg agccccgtgg tcggcgtccc gccggcggtc
     4081 acggatcttc acctggagct gacgcccgtt ccggggttcc gcacgacgac gctctcgagc
     4141 tcgttcgccg accggaccta cgaggaaccg gtgacgctga gcggcgcggt ctcctccgcc
     4201 accgacgggt gcgccgccgc ctccgtgcgc atctggcgcg acgtcctcgg cgaccaggtg
     4261 gacttccagg tgtatcgcga aacgacggcg ggaccgtccg gagccttctc ggttcccgtc
     4321 acacccgatg cgtctgccac gtaccgggcc gaagccgtgg cggagggcgc gtgcctcgcc
     4381 gccgagagcg cgacgcagac ggtgcgggtg gctgcgaagg tcggtctcaa ggcctcgagc
     4441 acccgcgtca gcaaggggac gacggtgaag ctgacggcca cggtcgcgcc ctgcccgggg
     4501 cacccggggc acgcggtcac gctcgtgcgg gggacgaagc agatcgggga gaagcagctc
     4561 ggaagcacct gcaaggccgt cttcaaggtg aagatgctga agacccggac gttcgaggcc
     4621 cgctacgagc agcagcacga cgaccatgac gcgggcatct ccaagcgtgt gaccgtgcgc
     4681 gtctgatccc ggacctcacc ccacgtacga cgcgcgggtc atcagcgacg gatccaccca
     4741 tgagcccggc ctggtcgagc tcgtggaagg cgccgaggtt cgcctccggc gaccgctctt
     4801 gccgcgcgcc caggcgtcgg ccgcgtgcac cgcggcctgc tggtggcgga ggtcgacgat
     4861 gcggatgccg gcacgaccgc agccgtcgag gatcggggcg tggtgacctc ccgccaccgt
     4921 gcaccaggtg ggtgatcccc cacgccacga ggacgtcggc ggcgcgatcg ccgccgtgcg
     4981 gctgcgtcac gccgactcga tcggcccgag gtagaccttc tcgccggcga acagctccgc
     5041 ctgcgggtcc cacgggaagt ggatcaccac ctggaagcgc ttctgtcgac cgtcgggctc
     5101 gaccccggcc cacggtccgt ggtgggtccc cgtcacgttc gccacctcga acaccccctg
     5161 cggcccgatc acgatgtcct ggaggtcgaa gtcgatgtcg gggaaggccg tgagcagctc
     5221 aaggtagaag cggcgggcgc cgtcgtgccc cttccagcgc tcccccgacc agaccagctc
     5281 gtagacgcag tccggcgtca gggtcgcgat caggccctcg aggtcgcgcg cgtcctccgc
     5341 cttcgagtgc tcgatccaca gccgccggat cttctcgtgc tcctcggagc tcgctgccca
     5401 tcccaggcgc tcccggatgt ccgcttcgct cggcatctcg cctcctcccc tcggtcggcg
     5461 cgagcgtagc gcacgcgggg gacccggcgg ccttcgctac agtggagccg tgcgacgacg
     5521 ccccccacgt agctcggccc cggccgcggc gaccccggac tggcgctcgt acgactcggt
     5581 cgccgagacg tacgagcggg tgcgcgcgcc gctccacgcc gacccggccg ccgacctcgt
     5641 gcgcctcatg gagctgccgc cgggtgggcg tgtcctcgac gtcggcacgg gccccggcgt
     5701 cgcggcggaa ctggcgggcg aggagctcgg ggccgagggg ctcgtggtcg gcgtcgaccc
     5761 gtcgatcggc atgctccgga cggcgatccg tcgggtcccg cacgtgatcc aggcgcaggc
     5821 gctggacctc cccttccgcg acggcgtctt cgacggcgtg atcgcgacct tcgtcgtgtt
     5881 cttcttccgc cggtacgaga ccggcctcgc cgagctgtct cgggtgctgc gccccggcgc
     5941 ggagctcggc ctgacgacct ggggcggtgg cgacgacgcg ttcgccgcga cgtggcgtga
     6001 gatcgccgag gcgtacgtcg ggcgggacct gctgcgcgac gccacgcgcc aggcggcgcc
     6061 gtgggaggag catttctcgg atcccggccg cgtgaaggag accctccgcg acgccggctt
     6121 ccgcaacgtg cgcgtcgagg tccgcaccta ccgccacacc gtgagcctcg aggactacat
     6181 cgcgggccgc gaaacgagcg tgaccggtcg gttcctccgc ggcatgctcg gcgaggcgct
     6241 ctgggcgcgc ttccacgccc gcgtcacgga ggagttccgc aaccggttcc cggatccgat
     6301 cggcgactcg aacgacgtcc tgctgacgct cgcccggaag ccctagcggc gcttgccgtt
     6361 cgcccgtccc tcgatgaacc gcacgagcgt gcggacgggg ctgccggtgg cgccgcgcgg
     6421 ccagtagtcg cccggccgct ccgagtacgc cgtcccggcg atgtcgaggt gggcccacgg
     6481 cacgtcgccg acgaagtgct tcaggaacag ggcggcggtg atcgttccgg cgccgcgcgc
     6541 accgatgttc ttcacctcag cgatctgcga ctcgatgtgc cgcttgtagc cgtcccacag
     6601 tgggagctcc cagccgggct cgccggccgc ctcacccgcg tcgagaagct cgcggacgag
     6661 tccgcggtcg ctgcccatca caccccagag ctcctcgccg agcgcgatca cgcaggcgcc
     6721 ggtgagcgtc gccgtgtcga cgatgacgga cggcttcttc tcggtgaggt acgcgagggc
     6781 gtcggcgagg atcagccggc cctcggcgtc ggtgttgagg acctcggagg tcttcccgcc
     6841 gcggtgccgc aggacgtcgc ccggacggat cgcggagccg cccggcatgt tctcggcgct
     6901 cgggatcgcc gcgacgacgt tgacgggcgg cttcagccgc gcgaccgcct gcatcgttcc
     6961 gaggatcgac gccgccccgg ccatgtcgca cttcatccac tccatgccct tcgcgtc
//
LOCUS       DATLGG010000225         4137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_230140, whole genome shotgun sequence.
ACCESSION   DATLGG010000225 DATLGG010000000
VERSION     DATLGG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230140"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VM638_06415"
     CDS             complement(<1..811)
                     /locus_tag="VM638_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVM12090.1"
                     /translation="MPRREPRPPQASHAGTAELLNGYRTTSLIVAAVQLGLPEHLAQG
                     PVSPEPLAERVDADADALVRLLRGLAALGLVEENDDGHALTDAGAELLPGRPLRDLAV
                     LVGEEYLPAWSRIADGVRTGRPAFPEVFGTGIWEHRERHPHLNDAFNRYLGRSEERAG
                     EAIASVLDLDAARVIVDLGGGLGGVLAGLLRSDPRLEGILVDLPHVIEGASARLDAAG
                     VLDRCRLVAGSFFERIPEGGDIYVLRYILHDWDDERAGAILRRCREAMHERA"
     gene            910..1773
                     /locus_tag="VM638_06420"
     CDS             910..1773
                     /locus_tag="VM638_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVM12091.1"
                     /translation="MPRPSDALTTDANPDHWPDLARRWSLVGPPLRVSAEDAAAYAEF
                     ARRILERPQPPRVLILGVTPELYGLPWPAGTLLRAADRSAAMARAVWPGPPEAVQIED
                     WTRLSFAPSSFDLALCDGGLHLLSYPDGQAGFVRSLRRVLAPGALLLLRLFVPPPEAE
                     AVDEVLRDLGAGRIGNVNELKIRLDMALQPDPVTGVRIGDVWEAFERAEPDLDALAAR
                     TGWSRESLGSIEAYRGSEATYHFVTERQVRRLFEVDPGGFTVEAVREPAYELGQRFPL
                     VALRRGPEDGG"
     gene            1770..2153
                     /locus_tag="VM638_06425"
     CDS             1770..2153
                     /locus_tag="VM638_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12092.1"
                     /translation="MSEQGRIWEAEWGPLGVVEGHDDVEGVLRWAVASDPVHVAVHRI
                     EGLGDDPPAYTSPHRHQDEELNLILGEDLVYEIRIGEDTSLVRAPGAVWVPSGVPHAA
                     NARGGRGHLICIRFTPGERPARGTR"
     gene            2242..>4137
                     /locus_tag="VM638_06430"
     CDS             2242..>4137
                     /locus_tag="VM638_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12093.1"
                     /translation="MNKSFVNRNRRPTRTMLVLAIGASIAAHPLHAPAASEAGEVRPV
                     YTVIDLGTFGGPLSNAFAVNDRSEVAGRGETPSLATKGFFWRDGRLTDIGALNASGNG
                     TTGLAINDRGEIAGDSMAPDPQFQGNSAMPFYWSEETGMINLGQDLTRTGTGRARGIN
                     NSGVVVGVWRKAAFRWSRAEGYREMEFLPDALWKQAEALAVNDAGVATGWATDPQGRV
                     RPVRWRPDGTIERLAEHATGWYGQGNEINASGAIAGEADRIIFDNVRRTRPVVWHPDG
                     SIEEIPLVQVDPPLDMGRADGINDRGEIVGMDSSSAWGDGRQVGWIRDPQGRKTVLND
                     LIDPSVGWDIRIPLDINNHGDIVGIGKLTKDGVEYPGRAFLMRKTVTVIGDSVRITGH
                     ARFAAPSQQVGASDRGSSPAGPAALGMDLASARLQQRDQHHLVAELGIHDMPENVPPE
                     VVHYEWPFEIRRGGQVVADLTLQAIRSAQAQRPGSLDPVFRLLECTPDPAGAQCTQRA
                     TLQGAFTSEGVRFVIPLSAIDGVAGGVMTQGRDVVSTSIGASGVRSGIAGDRMGVSDF
                     TIHPATVSLGIAPAGTPDHEVPLTRQATVYADGTYSGSVPRPTAPGEYVAVAKACLGP
                     DSCRLVSH"
BASE COUNT          591 a         1465 c         1529 g          552 t
ORIGIN      
        1 gtgcgcgctc gtgcatcgct tcgcggcagc gtcggaggat cgccccggcc cgctcgtcat
       61 cccagtcgtg aaggatgtag cgcagcacgt agatgtcgcc accctccggg atccgctcga
      121 agaacgagcc ggcgacgagg cggcagcggt cgaggacgcc ggccgcgtcg agccgggcac
      181 tcgcgccttc gatcacgtgc ggcagatcga cgaggatccc ctcgaggcgc ggatccgagc
      241 ggaggagccc cgccaggacg ccgccgaggc caccgccgag gtcgacgatc acccgtgctg
      301 cgtcgaggtc gaggacggag gcgatcgcct cgccggcgcg ctcctccgag cgtccgaggt
      361 accggttgaa ggcgtcgttg aggtgggggt ggcgctcgcg atgctcccag atccccgtcc
      421 cgaacacctc cggaaaggcc ggacgaccgg tgcggacgcc gtcggcgatc cgcgaccacg
      481 ccgggaggta ctcctcgccc acgaggacgg cgaggtcgcg caggggtcgt cccggcagca
      541 gctcggcccc cgcgtccgtc agcgcgtggc cgtcgtcgtt ctcctcgacg aggccgagcg
      601 cggcgagccc gcggaggagg cgcaccagcg cgtcggcatc tgcatcgacg cgctcggcga
      661 ggggctcggg ggagaccggg ccctgcgcga gatgctccgg caggccgagc tggacggcgg
      721 cgacgatgag cgatgtcgtc cggtacccgt tgagcagctc ggccgtcccc gcgtgggacg
      781 cctgcggcgg tcgtggctcg cgtcggggca tcgtctcggg aggcggttgg cggcgaggga
      841 cggcgcggct tacggtggcg gagtctagca gggcgagttt ctgaccgatc ccgcgaaccg
      901 gagccccgag tgccgagacc gagcgatgct ctgacgaccg acgcgaaccc cgaccactgg
      961 cccgacctgg ctcggcggtg gagcctcgtc gggccgcccc tgcgcgtctc ggcggaggat
     1021 gcggcggcgt acgccgagtt cgcgcggcgc atcctcgagc gcccgcagcc acctcgcgtc
     1081 ctgatcctcg gggtcacccc ggagctctac gggctcccat ggccggcggg aaccctcctg
     1141 cgggcggcgg atcggtctgc ggccatggcc cgcgccgtct ggccgggtcc ccccgaggcg
     1201 gtgcagatcg aggactggac ccggctttcc ttcgctccct cgtcgttcga tctcgccctg
     1261 tgcgacggcg ggctccacct gctgtcctac cccgacgggc aggcgggctt cgtccgctcg
     1321 ttgcggcggg tgctggcgcc cggcgccctc ctcctgctcc gcctcttcgt cccgccgccc
     1381 gaggcggaag ccgtcgacga ggtcctgcgg gatctgggcg ccggccggat cggcaacgtg
     1441 aacgagctga agatccgcct cgacatggcg ctgcaaccgg acccggtgac cggcgtgcgg
     1501 atcggtgacg tgtgggaggc gttcgagcga gccgaacccg acctcgacgc gcttgccgct
     1561 cggaccggat ggagccgcga gagcctcggc tcgatcgagg cctaccgagg ctcggaggcg
     1621 acctaccatt tcgtcacgga gcgtcaggtc cgccggctct tcgaggtcga ccccggaggg
     1681 ttcaccgtcg aggccgtgcg cgagccggcc tacgagctcg gccagcggtt cccgctcgtg
     1741 gcgctgcggc gcggcccgga ggacgggggt tgagcgagca gggacggatc tgggaggcgg
     1801 agtggggccc gctcggcgtg gtcgagggcc acgacgacgt ggagggggtt ctccggtggg
     1861 ccgtcgcgag cgatcccgtg cacgtggccg tccaccggat cgaaggactc ggggacgacc
     1921 cgcccgccta cacctcgccg caccgccacc aggacgaaga gctgaacctc atcctcggcg
     1981 aggacctcgt ctacgagatc cggatcggcg aggacacatc cttggtcaga gctccgggcg
     2041 ccgtctgggt cccgagcggc gtcccccatg cggcgaacgc ccggggcgga cgcggccacc
     2101 tcatctgcat ccgcttcacg ccgggagagc ggcctgcccg cgggacgcgc tgacgtccgc
     2161 cgttcgacaa ccggtccacg cgaaggacct cccgcccgat gtgctttggg cggatgcaca
     2221 aagggttccg cgcagcgcgg ggtgaacaag tccttcgtga accggaaccg gcgcccaacc
     2281 cggacgatgc tcgtgctcgc gatcggggca tccatcgctg cgcatccgct gcacgccccc
     2341 gccgcctcgg aggccggtga ggtgcggccc gtctacaccg tgatcgacct cggcaccttc
     2401 ggcggcccgc tgtcgaacgc gttcgcggtc aacgaccgga gcgaggtcgc gggccgcggg
     2461 gagacgccga gcctcgcgac gaagggcttc ttctggcgcg acggcaggct gacggatatc
     2521 ggggcgctga acgcgagcgg gaacggcacg acgggtctcg cgatcaacga ccggggcgag
     2581 atcgccggcg acagcatggc cccggacccg cagttccagg ggaactcggc gatgccgttc
     2641 tactggtccg aggagacggg catgatcaac ctcgggcagg atctcacccg gacgggcacg
     2701 ggacgcgctc gcgggatcaa caacagcggg gtggtcgtcg gcgtctggcg gaaggccgcg
     2761 ttccgctgga gccgggccga gggctaccgg gagatggagt tcctccccga cgcgctgtgg
     2821 aagcaggccg aggcgctcgc ggtcaacgac gcgggcgtcg cgacggggtg ggcgaccgac
     2881 ccccagggtc gcgtgcggcc cgtccgctgg cggcccgatg gcacgatcga gcgtctcgcc
     2941 gaacacgcga ccggatggta cgggcagggg aacgagatca acgcatccgg cgcgatcgcg
     3001 ggggaggccg accggatcat cttcgacaac gttcgccgga cgcggccggt cgtgtggcac
     3061 ccggacggct cgatcgagga gatcccgctc gtccaggtcg atccgcccct cgacatgggc
     3121 cgggccgacg ggatcaacga ccgtggcgag attgtgggca tggactcgag ctcggcgtgg
     3181 ggcgacgggc ggcaggtcgg atggatccgc gacccgcagg gtcggaagac cgtcctcaac
     3241 gacctgatcg acccgagcgt cggctgggac atccgcatcc cgctcgacat caacaaccac
     3301 ggcgacatcg tcggcatcgg caagctcacg aaggacggcg tcgagtaccc gggacgcgcg
     3361 ttcctgatgc gcaagaccgt gacggtgatc ggcgacagcg tgcgcatcac cggccacgcg
     3421 cggttcgcgg cgccgtccca gcaggtcggg gcgtccgacc gcggatcgag cccggccgga
     3481 ccggcggcac tcggcatgga cctcgcgtcg gcacggctcc agcagaggga ccagcaccac
     3541 ctcgtcgcgg agctcgggat ccatgacatg cccgagaacg tccctccgga ggtcgtccac
     3601 tacgagtggc cgttcgagat ccgccgcggg ggacaggtgg tcgccgacct gacgctgcag
     3661 gcgatccgaa gcgcgcaggc ccagcggccg ggatcgctcg atcccgtgtt ccgcctcctc
     3721 gagtgcaccc ccgaccccgc gggcgcccag tgcacccagc gtgcgacgct tcagggtgcc
     3781 ttcacgtccg agggcgtccg cttcgtgatc ccgctctcgg cgatcgacgg ggtcgccgga
     3841 ggggtcatga cccagggccg cgacgtcgtc tcgacgtcga tcggagcatc cggcgtgcgt
     3901 tccgggatcg cgggcgaccg gatgggcgtg tccgacttca cgatccatcc cgcgaccgtc
     3961 tcgctgggca tcgcgccggc ggggacgccg gaccacgagg tgcccctcac gcggcaggcg
     4021 accgtctacg cggacggcac ctacagcggc tcggtcccgc gcccgaccgc ccccggcgag
     4081 tacgtggccg tcgcgaaggc ctgcctcggc ccggacagct gccggctcgt gtcgcac
//
LOCUS       DATLGG010000226        10077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_230210, whole genome shotgun sequence.
ACCESSION   DATLGG010000226 DATLGG010000000
VERSION     DATLGG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230210"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VM638_06435"
     CDS             <1..388
                     /locus_tag="VM638_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HVM12094.1"
                     /translation="VKHREGFRPFAPSVLHERGSEFFEGYYPNPFMLLVLPIVPEKRD
                     VIPAVTHVDGTGRMQSVRREENPDYHRLISEFERLTGVPVVLNTSFNLRGEPIVARPE
                     EALEDYLNTGMDALLLGRYVLEKEPT"
     gene            524..676
                     /locus_tag="VM638_06440"
     CDS             524..676
                     /locus_tag="VM638_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HVM12095.1"
                     /translation="MASYEGYCVKDKKKVQFEGEVVTLKNGRQAAKGKCPTCGTTVMR
                     ILGKNA"
     gene            complement(722..1174)
                     /locus_tag="VM638_06445"
     CDS             complement(722..1174)
                     /locus_tag="VM638_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM12096.1"
                     /translation="MARILSVEDDDAIRGLIKHALTQYGYDVSEAASGPEAIDLLRRQ
                     EFDLAILDLMLGGEISGWDVYDEMKKMGIRDGMKVIIMTARSQEPEILKGWRLGVDQY
                     ITKPFDLDIFIVTVQDTLLSSKDQLAKHREQELRKTELLHMVDTVFNE"
     gene            complement(1217..1696)
                     /locus_tag="VM638_06450"
     CDS             complement(1217..1696)
                     /locus_tag="VM638_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HVM12097.1"
                     /translation="MFKVSQKTEYALRAMVELALRAGSSPVPAREISASQGIPLRFLE
                     QQLAALHKAGLVRSFRGAGGGCQLAREAESIPVAEVIDLMEGPSYPMVCLAPENDDCT
                     QTSHCGLQELWGEVHTAVREVFDRVTIGTLAARHRQLAPLNPMWSPEDLLRPVEQAR"
     gene            1810..2769
                     /locus_tag="VM638_06455"
     CDS             1810..2769
                     /locus_tag="VM638_06455"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HVM12098.1"
                     /translation="MESWFERRTFHHARFEDLDELRRAKETQGLTVSVCIPTLNEAAT
                     VGPIIDAVRRALVEDVPLVDELAIVDSASDDDTAGVAEAAGAVVFQDRDLVPDLPHLG
                     GKGDAMWKSLFALRGDLVLWIDADVEDFHPRFVYGLLGPLLLDPGVEYVKGFYERPLR
                     IGGETDPFGGGRVTELMARPLINAFWPDLAGIIQPLSGEYGGRRQLLERIPFFTGYAV
                     ELAMLIDILDASGLDAIAQVDLHRRTHRNRNIGDLSRMAYAILQGAAARLRGSGALAE
                     DAFVGGDLSLFAKEDGSYRIRPMAVPIHERPPAASVSGYQARG"
     gene            2766..3581
                     /locus_tag="VM638_06460"
     CDS             2766..3581
                     /locus_tag="VM638_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HVM12099.1"
                     /translation="MRAAAERRGRRPRVLYTDLDGTLLGPGGSLFAGSSGVTDAAASA
                     LTALHRAGIEVVPLSGRTEEQVREVARLLGATSFIAELGGIVWMGERIARYGAHPGPG
                     TPYEAMARSGAVAFLLERSAGRLEPHAPWAFRGREVTMLLRGHVDPSEANTSLAEAGY
                     GWLRLVDNGQIPVPGPTDVVHAYHLAPEGVTKADAVAEHLRRRGLHAGDAVAIGDAPA
                     DAAVADHVDAVYIVANGAASVEAAGAGRENVYVTPSERGEGFAEAVALLLEAA"
     gene            complement(3578..4837)
                     /locus_tag="VM638_06465"
     CDS             complement(3578..4837)
                     /locus_tag="VM638_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVM12100.1"
                     /translation="MQDEPNRVLATPLVIRAARWGVVAWAAIGVSALTIAFFAFVVYP
                     IRIIFPPLVVALIAVFLLNPIVSRLEARGMPRLLGALLTYSIFVAIVALGLRFLIPIV
                     VAQAQEFGETVPALVDRLQRWTQGVAGGLGFDVAGETGGRQVLDFLGGLLSFTRGLFH
                     VALVLVLGPILAFYLLVDLPKIRRGIEALIPERRRDEVHTVARELSHALGGFFRGQLL
                     VAASVGIGSAIALFIVGLPYWAVVGLVAGLFNLIPLIGPFIGGLVAIAIAFTTDQSGG
                     LLSLAPGVPLAVGSAIALTIVQQLDNHILSPNIVARTVKLHPVTVMLALLAGGTLLGL
                     WGMLLAVPVVASVKVLALHAWDTRMTWPPPPPDAQTEPPSAAVAGPPEDPPPPSPAAP
                     PPRSPRPRRWWPWPERSGSAGEGRRRT"
     gene            4930..5376
                     /locus_tag="VM638_06470"
     CDS             4930..5376
                     /locus_tag="VM638_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM12101.1"
                     /translation="MREGGVLRILVIDDEPDVLLLCRVNLHFEGHEVIEAGDGERGLE
                     RAREEQPDLIVLDVMLPQRDGFSVLQRLTADPRTSEIPVILLTAKAQEDDQMRGWLAG
                     AAEYVTKPFSPVALTDAVRSVLSMTPAERSARRAKNLAQLSILHRI"
     gene            5421..6659
                     /locus_tag="VM638_06475"
     CDS             5421..6659
                     /locus_tag="VM638_06475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12102.1"
                     /translation="MLVSVPLPEPKRADDLRAAAGDEAIDRLKAAAEPFAGARIVHIS
                     STAFGGGVAELLNTQIALLQDLGIDAEWQLMEGSEEFFAVTKLVHNALQGAGVPWTEA
                     METTYLDRVAVNAARFDASADFVFVHDPQPAAMLTFLEEQGRRSGKWVWRCHIDLSQP
                     MEAVWGFFALHVARYDAAVFTTEGFVRPGLPIRSAIIPPAIDPLAPKNTLLDDDAVTE
                     ILRGYSIDARRPIVTQVSRFDPWKDPLGVIDAYRIARERIPDLQLVMVGSMAHDDPEG
                     WHYLERTEEHRAGDPDIHILTNLQGVGDVAVNAFQRASQVVVQKSLREGFGLTASEAM
                     WKERPVIAGNVGGLRLQVEEGVTGHLVDSVEECAERMIGLLEDEELRDKMGRAGRQRV
                     QERFLTPREVQDYLEVLTEL"
     gene            6656..8116
                     /locus_tag="VM638_06480"
     CDS             6656..8116
                     /locus_tag="VM638_06480"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVM12103.1"
                     /translation="MTARRILVASNRGPVSYVAGDDGALVPKRGAGGLVTALSGAVQT
                     TGGLWVASAMSAADREMAASTPTGRLDPVVDEAKYALRLIAEDPQTYDRFYNGISNHM
                     LWFLHHALWNLPYAPTLDSGTRDDWEAYRRVNGRFAEALAEEIDRNPADVLVQDYHLS
                     LVPAALRARAAHARIAYFHHIPFAGSVELSLLPGWIRRDLLTGLLGADLVGFQAARWA
                     ENFLTACRSLEDAQVDTRRRQVRWEDRIVQVGVYPITVETDSLLETADSPAVAARTTD
                     LERWLGGRRLLLRVDRTEPSKNILRGFQAYEEFLRRYPGWRRKVCFLALLNPSRQSLP
                     EYRRYTDACREAAEAINAAFGEPDWRPVELRINDDFDEVVAAYGLYDVLLVNPVLDGM
                     NLVAKEGPLLNRRDGVLLLSRTAGAFAELGRQALALDPLDVTATADAINAALRMPAGE
                     RARRAGGLRRAAGAGSPADWVARQMADLDRAATREE"
     gene            complement(8118..8876)
                     /gene="otsB"
                     /locus_tag="VM638_06485"
     CDS             complement(8118..8876)
                     /gene="otsB"
                     /locus_tag="VM638_06485"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVM12104.1"
                     /translation="MPQTIDAIRERRERAGILLDFDGTLARIVPTPEEARPVPGGVEV
                     LRELRHRYRSVAVVSGRRAADVWERIEGAARCFGLYGLEDERGPTRWALEPRPDLDEV
                     VEQVRTIAAAVPGARLEDKGPHVAIHYRAARDPGAGSLLRSRLLPLADRAGLRLLEGK
                     QVLELAPRGGPTKGDVVREVARADELQAILFAGDDLGDVDAFAAVDELAQVPSCVGVT
                     VAVRSAETPPAVVDAATTSVEGPERLVELLRSLL"
     gene            8910..9842
                     /locus_tag="VM638_06490"
     CDS             8910..9842
                     /locus_tag="VM638_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12105.1"
                     /translation="MRRLLLIPLLASLTACLPDEVTLKYGTEPGRRLTYELRLRARID
                     RTLAGEERRQTVAATFRAQQQITGRSPQGGATALMTLEPRSLTVDGRGQQVGAPQSFE
                     VRIAADGRILQIQRTGEGDAAAEEAEPVAAPTPGPPLASPAADPTPQPPDGTDDPGTG
                     EEQLAPLGIDRLIPRLRPVLPGRPVSPGETWRSVSDFQDEGGRFSLELRSRLAALGLR
                     EDRRAALVRTTYSSPVRRREIFSNAVTDIQGEDVGSQEAWFALDGYLIEARGDSVGVY
                     RLLFRPPGGSGAGLEPVEGSLRVRLSTEMRLVGR"
     gene            complement(9839..>10077)
                     /locus_tag="VM638_06495"
     CDS             complement(9839..>10077)
                     /locus_tag="VM638_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HVM12106.1"
                     /translation="SGLAASAADELQQYGYEISGVGNAQRNYRVTTLFYQPDAQAAAE
                     QLAADFFPEARLEAANNNLSPNIQVTVVIGEDAL"
BASE COUNT         1555 a         3490 c         3597 g         1435 t
ORIGIN      
        1 ggtgaagcac cgggaggggt tccggccgtt cgcccccagc gtcttgcacg agcgcggcag
       61 cgagttcttc gagggctact accccaaccc attcatgctc ctcgtgctgc cgatcgtgcc
      121 cgagaagcgc gatgtcatcc cggccgtgac ccacgtcgac ggcacgggac ggatgcagag
      181 cgtccgccgg gaggagaacc ccgactacca ccggctcatc tccgagttcg agcgcctgac
      241 cggcgtgccc gtggtgctga acacgagctt caacctccgc ggtgagccga tcgtggcgcg
      301 tccggaggaa gccctcgagg actacctgaa cacggggatg gatgctctgc tgctcgggcg
      361 gtacgtgctc gagaaagagc ccacctgacc ggaacgcggg atacggccgg ttcatctcgc
      421 tcgtcaggct tcttgccgga ggtgcttgcg cttccccgct cgaggcgaca cgattccgcc
      481 gtggagccgg gatcacggct ccacgtcgat gaaagggggc tcgatggcca gctacgaggg
      541 ctactgcgtg aaggacaaga agaaggtcca attcgagggt gaggtcgtga cgctgaagaa
      601 cggccggcag gcggcgaagg gcaagtgccc gacctgcggc acgaccgtga tgcggatcct
      661 cggcaagaac gcctgaggcg accggacccc acccgcgcgg ggccggtgcg cgggacgccg
      721 tctactcgtt gaagacggtg tcgaccatgt gcaggagctc ggtcttgcgg agctcttgct
      781 cacggtgctt cgccagctga tccttcgagg acagcagcgt gtcctggacg gtgacgatga
      841 agatgtccag gtcgaagggc ttcgtgatgt actggtcgac gccgaggcgc cagcccttca
      901 ggatctcggg ctcctggctc cgcgcggtca tgatgatgac cttcatcccg tcgcggatcc
      961 ccatcttctt catctcgtcg tagacgtccc agcccgagat ctcgccgccg agcatcaggt
     1021 cgaggatcgc gaggtcgaac tcctggcgac gcagcaggtc gatcgcctct ggcccggacg
     1081 ccgcctcgga gacgtcgtag ccgtactggg tcagggcgtg cttgatcagg ccgcggatcg
     1141 cgtcatcgtc ctcgacgctc aggattcgtg ccatcggagt aggtatcggc gggtcgcggc
     1201 cgatattgag agcctatcag cgggcctgct cgacggggcg gagaaggtcc tccggcgacc
     1261 acatcgggtt gagcggggcg agctgacggt ggcgagcggc gagggtcccg atcgtgacgc
     1321 ggtcgaacac ctcgcgaacc gcggtgtgaa cctctcccca cagctcctgg aggccgcagt
     1381 gggaggtctg ggtgcagtcg tcgttctccg gcgcgaggca caccatcggg taggacgggc
     1441 cctccatcag gtcgatcacc tcggcgacgg ggatcgactc cgcctctcgc gcgagttggc
     1501 acccaccgcc ggcgccacgg aaggagcgga cgagaccggc cttgtgcagg gcggcgagct
     1561 gctgttcgag gaaccgcaag gggatcccct gggacgcgga gatctcgcgg gcggggacgg
     1621 ggctgcttcc ggcccggaga gcgagctcga ccatcgctcg gagcgcgtac tccgtcttct
     1681 gcgagacctt gaacatcgga cctcctccgg agtggcccct tttgggagtt gccgtgaatc
     1741 atatcacagg ggtcggattc ggtcacgcac gacccgggcg gtcgagcgac cacgtatatc
     1801 atccggcgga tggagagctg gttcgagcgg cggacgttcc accatgctcg gttcgaggac
     1861 ctcgatgagc tgcggcgcgc caaggagacg caggggctca cggtgtccgt ctgcatcccc
     1921 accctgaacg aggcggcgac cgtcggaccg atcatcgacg cggtgcggcg cgcgctcgtg
     1981 gaggacgtcc ccctcgtcga tgagctcgcg atcgtcgatt ccgcctcgga cgatgacacg
     2041 gcaggggtgg ctgaggccgc cggcgcagtc gtcttccagg accgcgacct cgtccccgac
     2101 cttcctcatc tcgggggaaa gggcgacgcg atgtggaaga gcctcttcgc gctccgaggg
     2161 gacctcgtcc tgtggatcga tgccgacgtc gaggacttcc acccgcgatt cgtctacggg
     2221 ctgctcggac ccctcctgct cgacccgggg gtcgagtacg tcaagggctt ctacgagcgg
     2281 ccgctacgga tcgggggcga gaccgacccg ttcggcggcg gacgcgtcac ggagctcatg
     2341 gcgaggccgc tcatcaacgc cttctggccc gacctcgcgg ggatcatcca gcccctgtcc
     2401 ggtgagtacg ggggacgccg tcagctgctc gagcggatcc cgttcttcac cgggtacgcg
     2461 gtggagctgg cgatgctcat cgacatcctc gacgcatcgg gcctcgacgc gatcgcccag
     2521 gtcgacctgc accggcggac ccaccgcaac cggaacatcg gtgacctgag ccgcatggcg
     2581 tacgcgatcc tgcagggcgc ggcggcgcgc ctccgcggat cgggcgccct cgccgaagac
     2641 gcgttcgtcg ggggcgacct cagcctcttc gcgaaggagg atggctcgta ccggatccgc
     2701 ccgatggcgg tcccgatcca cgagcgccca ccggcagcat ccgtcagcgg gtaccaggcc
     2761 cgaggatgag ggcagcggcg gagcggcgcg gacgccgccc ccgtgtcctc tacaccgacc
     2821 tcgacggaac gctcctcggc cccggggggt ccctgttcgc cgggtcttcc ggcgtcaccg
     2881 acgccgccgc ctccgccctg acggcgctcc accgagcggg gatcgaggtg gtcccgctgt
     2941 cgggacggac ggaagagcag gtccgggagg tggcgagact cctcggagcg acgtcgttca
     3001 tcgcggagct cggcggcatc gtctggatgg gtgagcggat cgctcgctac ggcgcccacc
     3061 ccggccccgg cacgccgtac gaggcgatgg cgcgcagcgg agccgtcgcc ttcctgctcg
     3121 agcgatccgc cgggaggctc gagccccacg ccccctgggc gttccgcgga cgcgaggtga
     3181 cgatgctcct ccgtgggcac gtcgaccctt cggaggcgaa cacgagcctc gctgaggcgg
     3241 gctacggctg gctccggctc gtggacaacg ggcagatccc ggtccccggt cccaccgacg
     3301 tcgtgcacgc gtaccacctc gcgcccgaag gcgtcacgaa ggcggatgcg gtcgcggagc
     3361 acctccggcg acgcggtctt cacgcggggg atgccgtcgc gatcggcgat gcgccggccg
     3421 acgcggccgt ggccgatcac gtcgacgcgg tgtacatcgt cgcgaacggc gccgcttcgg
     3481 tggaggcggc gggagcgggg cgggagaacg tgtacgtgac gccctccgag cggggcgagg
     3541 ggttcgcgga ggcggtggcc ctcctcctgg aagcggccta ggtcctgcgg cgtccctcgc
     3601 cggccgatcc ggagcgctcc ggccacggcc accaccgacg cggccgcggc gatcgaggcg
     3661 gaggcgcagc cggagacgga ggtggtggat cctcgggcgg cccggcgacc gcagccgacg
     3721 gcggctccgt ctgggcgtcg ggtggcggtg gcggccacgt catccgggtg tcccacgcgt
     3781 gcagcgccag caccttcacc gaggcgacca ccggcaccgc gagcagcatg ccccacagcc
     3841 cgagcagcgt gccgcccgcg aggagcgcga gcatgaccgt gaccggatgc agcttcacgg
     3901 tccgcgcgac gatgttgggg ctgaggatgt ggttatccag ctgctgaacg atcgtgagag
     3961 cgatcgccga acccaccgcg agcggcacac cgggagcgag gctcaggagc cctccgctct
     4021 gatcggtcgt gaacgcgatc gcgatcgcga cgaggccgcc gatgaacggg ccgatcagcg
     4081 ggatgagatt gaagagcccc gccacgagtc cgacgaccgc ccagtacgga aggccgacga
     4141 tgaacagcgc gatcgcggag ccgatcccga cggatgccgc gacgagcagc tgcccgcgga
     4201 agaacccgcc gagcgcgtgg gacagctcgc gtgcgacggt gtgcacctcg tcccgacgac
     4261 gctccgggat cagcgcctcg atcccgcgcc ggatcttcgg gaggtcgacg agcaggtaga
     4321 aggcgaggat cggcccgagc accagcacga gggcgacgtg gaagagaccg cgcgtgaacg
     4381 acaggaggcc tccgaggaag tcgagcacct gccggccacc cgtttcacca gccacatcga
     4441 agccgaggcc gccggcgacc ccctgcgtcc accgctgcaa ccggtcgacg agggcaggga
     4501 ccgtttcgcc gaactcctgc gcctgcgcga cgacgatggg gatcaggaaa cgcagcccca
     4561 gggcgacgat cgcgacgaag atcgaatacg tgaggagcgc gccgagcagg cgcggcatcc
     4621 ccctggcctc cagccgcgag acgatcgggt tcaggaggaa caccgcgatc agggcgacca
     4681 cgagcggcgg gaagatgatc cggatcggat agacgacgaa cgcgaagaac gcgatcgtca
     4741 gcgccgacac gccgatcgcg gcccaggcca cgacccccca gcgcgcggcc cggatgacca
     4801 gaggggtcgc gagcacccgg ttcggctcgt cctgcacgtg ctcattctga cacgcgcacc
     4861 ggcgtgggcc acggggaaat ggcccgaacg ggccattcac ctggtcccgt cagctcccga
     4921 tgctggcgta tgagggaagg aggcgtcctg cggatcctgg tgatcgatga tgagcccgac
     4981 gtgctgctgc tctgccgcgt caaccttcat ttcgaagggc acgaggtgat cgaggccggc
     5041 gacggcgaga ggggactcga acgggcccgc gaggagcaac ccgacctcat cgtcctcgac
     5101 gtcatgctgc ctcagcgcga cgggttctcc gtgctgcagc gactcaccgc cgacccgcgc
     5161 acgagcgaga tcccggtcat cctgctgacc gcgaaggcgc aggaggacga ccagatgcgc
     5221 ggctggctcg ccggcgccgc cgagtacgtg acgaagccgt tcagccccgt ggcgctgacg
     5281 gacgccgtcc gctcggtcct gtcgatgacg cccgccgaac gatcggctcg gcgcgcgaag
     5341 aacctggcgc agctctcgat cctgcaccgc atctagctcc gcttggaggg cccccgtcgg
     5401 gccgctgtat ggtggaggcg atgctcgtca gcgtccccct tccggagccg aaacgcgccg
     5461 acgacctccg agcagcagcc ggcgacgagg cgatcgaccg cctgaaggcg gcggccgagc
     5521 ccttcgccgg ggcgcggatc gtccacatca gcagcaccgc gttcggtggc ggcgtggccg
     5581 agctcctgaa cacccagatc gcgctcctgc aggacctcgg tatcgacgcg gagtggcagc
     5641 tgatggaggg cagcgaggag ttcttcgccg tcacgaagct cgtccacaac gccttgcagg
     5701 gtgcgggcgt gccgtggacc gaagcgatgg agacgaccta cctcgatcgc gtcgccgtca
     5761 atgccgcccg gttcgacgcg tcagccgact tcgtgttcgt ccacgatccc cagcccgcgg
     5821 cgatgctgac gttcctcgag gaacaggggc ggcgctcggg gaagtgggtc tggcgctgcc
     5881 acatcgacct ctcgcagccc atggaggccg tgtggggctt cttcgccctc cacgtcgcgc
     5941 gctacgacgc ggcggtgttc accaccgagg ggttcgtccg gcccgggctg ccgatccgga
     6001 gcgcgatcat cccgccggcg atcgacccgc tggccccgaa gaacaccctg ctcgacgacg
     6061 acgccgtcac cgagatcctg cggggatact cgatcgacgc ccggcggccg atcgtcacgc
     6121 aggtgtcccg gttcgatccc tggaaggacc cgctcggtgt catcgacgcg taccgcatcg
     6181 cccgcgagcg gatccccgat cttcagctcg tcatggtcgg gtcgatggcg cacgacgacc
     6241 ccgagggctg gcattacctc gagcgcacgg aggagcaccg cgccggtgac cccgacatcc
     6301 acatcctgac gaacctccag ggggtcggcg acgtggcggt caacgccttc cagcgcgcgt
     6361 cacaggtggt cgtgcagaag tccctgcggg agggcttcgg cctgaccgcc tcggaggcga
     6421 tgtggaagga gcgtccggtg atcgccggca acgtgggcgg cctccggctc caggtcgagg
     6481 agggggtcac cggccacctc gtggactccg tggaggaatg cgccgagcgg atgatcggcc
     6541 tgctcgagga cgaggagctc cgggacaaga tggggcgcgc ggggcgccag cgcgtgcagg
     6601 agcgtttcct gacgccccgg gaggttcagg attacctgga ggtgctcacg gagctgtgac
     6661 cgcacggcgg atcctcgtcg catcgaaccg ggggccggtc tcgtacgtgg ccggggacga
     6721 cggcgccctc gtcccgaagc gcggggcggg cggcttggtg acggcgctga gcggggcggt
     6781 ccagacgacg ggcgggctct gggtcgcctc ggcgatgagc gcggcggacc gcgagatggc
     6841 ggcctccacg ccgacgggcc gcctcgatcc cgtcgtcgac gaagcgaaat acgcgttgcg
     6901 cctgatcgcg gaggatccgc agacgtacga ccgcttctac aacggcatca gtaaccacat
     6961 gctgtggttc ctccatcacg ccctttggaa cctgccgtac gcgccgacgc tcgacagcgg
     7021 cacgcgtgat gactgggaag cgtaccgacg ggtgaacggg cgcttcgctg aggctctcgc
     7081 cgaggagatc gaccgcaacc ccgccgacgt cctcgtgcag gactaccacc tctcgctcgt
     7141 tcccgccgcg ctccgggcca gggctgccca cgctcggatc gcgtacttcc atcacatccc
     7201 gttcgccggc agcgtcgagc tcagcctgct ccccgggtgg atccgacgcg acctgctcac
     7261 ggggctcctc ggtgcggatc tcgtgggctt ccaagccgcg cgctgggccg agaacttcct
     7321 cacggcctgc cggtcgctcg aagacgccca ggtggacacg cgccggcgac aggtccggtg
     7381 ggaggaccgc atcgtgcagg tcggggtgta cccgatcacg gtcgagacgg attcgctgct
     7441 ggagaccgcg gacagcccgg cggtggcagc gcggacgacc gacctggagc ggtggctcgg
     7501 gggccgccgc ctgctcctgc gggtcgaccg gacggaacca tcgaagaaca tcctgcgtgg
     7561 cttccaggcc tacgaagagt tcctgcgccg ttaccccggg tggcgacgga aggtctgctt
     7621 cctcgcgctc ctcaacccct cgaggcagag cctgccggag taccgccgct acaccgacgc
     7681 ctgtcgcgaa gcagcggaag ccatcaacgc ggcgttcggc gaaccggact ggcgtccggt
     7741 ggagcttcgg atcaacgacg acttcgacga ggtggtcgcg gcgtacgggc tctacgacgt
     7801 cctcctcgtg aaccctgtgc tggacgggat gaacctcgtc gcgaaggagg ggccgctgct
     7861 gaaccggcgc gacggcgttc tcctgctctc ccggaccgcg ggtgccttcg ccgagctggg
     7921 ccggcaggcg ctcgccctcg atcccctcga cgtcaccgcg accgcggacg cgatcaacgc
     7981 ggcgctgcgg atgccggcgg gtgaacgcgc gcggcgggcc ggcgggctgc gacgggcggc
     8041 gggcgctgga tcgccggcgg actgggtggc gcgccagatg gcggacctcg accgcgcggc
     8101 gacgcgcgag gagtagatca gagcagcgag cgcaagagct cgacgagtcg ttcggggcct
     8161 tccacactgg tcgtcgcggc atccacaacg gccggaggcg tctccgccga gcggaccgcg
     8221 accgtgaccc cgacgcagga cgggacctgg gccagctcgt ccacggcggc gaaggcgtcg
     8281 acgtcgccaa ggtcgtcgcc ggcgaagagg atcgcctgca gctcgtccgc ccgcgccacc
     8341 tcccgcacga catctccctt cgtcggtcca ccgcggggag cgagctcgag gacctgcttg
     8401 ccttcgagca accggagacc cgcgcggtcg gcgagcggca ggaggcgcga gcgcaggagc
     8461 gagcccgccc ccgggtcgcg cgctgcgcgg taatggatcg cgacgtgcgg gcccttgtcc
     8521 tcgaggcggg ctcccggcac ggcggccgcg atcgtccgaa cctgctcgac gacctcgtcg
     8581 aggtcgggtc ggggctcgag cgcccagcgg gtgggcccgc gctcgtcctc gagcccgtag
     8641 agcccgaagc accgagccgc cccctcgatc cgctcccaca cgtcggccgc gcgccggccc
     8701 gagacgacgg cgacgctgcg gtagcggtgg cggagctccc gcagcacctc gaccccgccc
     8761 ggcacgggac gcgcttcctc gggcgtgggc acgatccgtg cgagggttcc atcgaagtcg
     8821 agcaggatcc ccgcacgctc gcgtcgctcc cggatcgcgt cgatcgtctg cggcatctcc
     8881 ggaccctacc cttgctagac tcgggatgcg tgcgccgcct cctcctgatc ccgctccttg
     8941 cgtccctcac ggcgtgtctc ccagacgagg tgaccctcaa gtacggcacg gagccgggac
     9001 gacgcctcac ctacgagctg cgcctgcgcg ccaggatcga ccgcaccctc gccggggagg
     9061 aacggcggca gacggtcgcc gcgacgttcc gtgcgcagca gcagatcacc ggtcgatcgc
     9121 cgcagggggg agccaccgcg ctgatgaccc tcgagccgcg gtcgctgacc gtcgacggcc
     9181 gtgggcagca ggtcggcgcg ccacagtcgt tcgaggtgcg gatcgccgcc gatggccgga
     9241 tcctgcagat ccaacggacg ggcgagggcg atgccgcggc ggaagaggcc gagcccgtgg
     9301 cggcaccgac gcccggacca ccgctcgcct cgcccgccgc ggatccgacg ccgcagccgc
     9361 ccgacggaac ggacgatccc gggacgggag aggaacagct ggctccgctc gggatcgacc
     9421 gcctgatccc gcgcctccga ccggtcctgc cggggcggcc ggtcagcccc ggagagacct
     9481 ggcgctcggt ctcggacttc caggacgagg gcggccgctt ttccctcgag ctgcgctcgc
     9541 gtcttgcggc tctcgggctg cgggaggacc gtcgcgccgc cctcgttcgc accacctact
     9601 cgtcccccgt tcggaggcga gagatcttct cgaacgccgt gacggacatc cagggcgaag
     9661 acgtcggcag tcaggaagcc tggttcgccc tcgacgggta cctgatcgag gcccgcgggg
     9721 attccgtcgg cgtgtaccgc ctcctgttcc gcccgccggg tggcagcggg gcgggcctcg
     9781 agccggtgga gggaagcctg cgggtgcggc tcagcaccga gatgcggctg gtcggacgct
     9841 agagggcgtc ctcgccgatc acgaccgtca cctggatgtt cgggctgagg ttgttgttgg
     9901 cggcctcgag gcgggcctcc gggaagaagt ccgcggcgag ctgctccgcc gctgcctgcg
     9961 cgtcgggctg gtagaagagc gtcgtcacac ggtagttccg ctgggcattg cccacgccgc
    10021 tgatctcgta gccgtactgc tggagctcgt cggcggcgga agcggcgagc cccgact
//
LOCUS       DATLGG010000227         2925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_61066, whole genome shotgun sequence.
ACCESSION   DATLGG010000227 DATLGG010000000
VERSION     DATLGG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61066"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VM638_06500"
     CDS             complement(<1..653)
                     /locus_tag="VM638_06500"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HVM12107.1"
                     /translation="MARIGVLTGGGDCPGLNAAIRAIVRKGVEHHGDHLIGFRNGYEG
                     VLEGSALELGTGAARGILHRGGTILGTSRVDPFRVDGGPDRIREGLEAQRIDGLIVIG
                     GEGTLGTAAKLWAEGIPIVGVPKTIDNDLAVTDLTFGFDTAVQVATDAIDRLHTTAES
                     HNRVMVIEVMGRSAGWIGLHAGLAGGADVILIPEQPFDVGKVCAHLRNRPEEGHFFS"
     gene            754..1782
                     /locus_tag="VM638_06505"
     CDS             754..1782
                     /locus_tag="VM638_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVM12108.1"
                     /translation="MRKIALALAAASLALPALAAPPKQRDVERARQARDQAQATVRAL
                     TAEAAALTERFHELGARADRAAARVVDAYRAELELEGQMAEARTTLNERASAAYRIGP
                     AAMVSVFLGSDSPADFLASQELIEHAIYGDIREATEILERSEEATARRAQVERERQKL
                     LLRHRQLQELRAQIEDRLVRAESAAKAAGLRLAAVQEARKRFLAEQQAQEEAVRLIGG
                     VDQSELLKLLGPNGGRGCEIPPRLRNTGRTISGTASWYGWDFAGRPTATGATYDPRLF
                     TAAHKTLPLNSFIRVRWNGRCAILLVNDRGPFHDDWILDLSQAAARYLGYERSGTAEV
                     TADLLARR"
     gene            1805..>2925
                     /gene="glpK"
                     /locus_tag="VM638_06510"
     CDS             1805..>2925
                     /gene="glpK"
                     /locus_tag="VM638_06510"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HVM12109.1"
                     /translation="MPHVLSIDAGTTGVTALLVREDGEIAARGYREFPQSFPRPGWVE
                     HNPEDWWRAALAATGEALAHARLAPRDVTAVGITDQRETTVVWDRRDMRPLHPAMVWQ
                     DRRTAPLCEELREEGWEERIRERTGLVIDPYFSATKLAWLLQEVDGLRAEAEGGRAAF
                     GTVDSYLIARLTGGRVHATDHTNASRTMLFDIHRLDWDDDILERLRIPRTMLPEVLPS
                     SGAFGTTDPDAFLGIEAPIAGVAGDQQAALVGQACFSPGDVKNTYGTGSFVLLNTGER
                     ATVSREGLLTTIAYSDADGARYALEGSIFVTGAAIQWLRDGLGILERSSDAEPLARTV
                     PDTGGVYFVPALTGLGAPWWDPYARGTIVGITRGTTRAHL"
BASE COUNT          409 a         1120 c         1012 g          384 t
ORIGIN      
        1 atcgagaaga agtggccttc ctcaggccgg ttccgcaggt gggcgcacac cttgccgacg
       61 tcgaacggct gctcggggat caggatcacg tcggcgccac cggcgagacc cgcgtgcagg
      121 ccgatccagc ccgcgctccg ccccatcacc tcgatcacca tcacgcggtt gtggctctcg
      181 gcggtcgtgt ggaggcggtc gatcgcgtcg gtcgcgacct gcacggctgt gtcgaacccg
      241 aaggtgaggt cggtgacggc gaggtcgttg tcgatcgtct tggggacgcc cacgatcggg
      301 atcccctcgg cccagagctt cgccgcggtc ccgagcgtcc cctcgccgcc gatcacgatc
      361 agcccgtcga tccgctgggc ttcgagcccc tcgcggatcc gatccggtcc gccgtcgacc
      421 cggaacgggt ccacccgcga ggtgccgagg atcgtccccc cgcgatgcag gatcccgcgg
      481 gcggcccccg tcccgagctc gagcgcggac ccctcgagca cgccctcgta gccgttccgg
      541 aagccgatca ggtggtcccc gtggtgctcc acccccttgc ggacgatcgc gcggatcgcc
      601 gcgttcagcc ccggacagtc gccgcccccg gtgaggacgc cgatcctcgc cacatctgct
      661 ccttcccctc gaccggagcc catcctacgt cggcccccct cggggccgat aagatggagg
      721 agcccgaacc cccctgaagc ctggagatcc ggcatgcgga agatcgcact cgcgctcgcg
      781 gcggcctcgc tggcgctgcc ggccctcgcg gcacccccga agcagcggga cgtcgagcgg
      841 gcgcgacagg cgcgggacca ggcgcaggcg accgtccgcg cgctgacggc cgaggcggca
      901 gccctgacgg agcggttcca cgagctcggc gcgcgggccg accgggcggc tgctcgggtc
      961 gtcgacgcct accgggcgga gctcgagctc gagggacaga tggccgaggc ccgaaccacg
     1021 ctgaacgagc gagcgagcgc cgcctaccgg atcggcccgg cggcgatggt ctcggtcttc
     1081 ctcggctccg acagccccgc cgacttcctc gcgagccagg agctgatcga gcacgcgatc
     1141 tacggcgaca tccgcgaggc caccgagatc ctcgagcgct ccgaggaggc cacggcgcgg
     1201 cgggcacaag tcgaacggga gcgccagaag ctcctcctgc gccaccggca gctgcaggag
     1261 ctccgcgcgc agatcgaaga tcgcctcgtg cgggcggagt cggccgcgaa ggccgctggt
     1321 ctgcggctcg ccgccgtgca ggaggcgcgg aagcgcttcc tcgccgagca gcaggcccag
     1381 gaggaggccg tgcgcctgat cggcggcgtc gaccagtcgg agctgctgaa gctcctcggg
     1441 ccgaacggcg gtcgcggctg cgagatcccg ccgaggctcc ggaacaccgg ccggacgatc
     1501 tccggcaccg cgtcctggta cggctgggac ttcgccggcc ggccgaccgc gacgggcgcg
     1561 acgtacgacc cgcggctgtt caccgcggcc cacaagaccc tgccgctgaa ctcgttcatc
     1621 cgcgtgcgct ggaacggccg ctgcgcgatc ctgctcgtca acgaccgcgg gccgttccac
     1681 gacgactgga tcctcgacct ctcccaggcc gccgcccgtt acctcggcta tgagcgctcg
     1741 ggtaccgccg aggtcaccgc cgacctcctc gcccgccgct agccgaggcc ggaagcctcc
     1801 cggcatgccg cacgtgctgt cgatcgatgc gggcacgacc ggcgtcacgg cgctgctcgt
     1861 gcgggaggac ggcgagatcg cggcgcgcgg gtaccgggag ttcccgcagt cgttcccgcg
     1921 gccggggtgg gtcgagcaca acccggagga ctggtggcgg gccgccctcg ccgcgacggg
     1981 tgaggcgctg gcccacgcgc gactggcgcc gcgcgacgtg accgccgtcg gcatcaccga
     2041 ccagcgtgag acgaccgtcg tctgggaccg ccgggacatg cgcccgctcc accccgcgat
     2101 ggtctggcag gaccgccgaa ccgccccgct gtgcgaagag ctgcgtgagg aagggtggga
     2161 ggagcgcatc cgcgagcgga cgggcctcgt gatcgatccg tacttctcgg ccaccaagct
     2221 cgcctggctg ctccaggagg tcgacggcct gcgcgcggag gccgagggag gccgcgccgc
     2281 cttcgggacc gtcgactcct acctgatcgc gcgcctcacc ggcggccgcg tccacgccac
     2341 cgatcacacg aacgcctccc gcacgatgct cttcgacatc caccgcctgg actgggacga
     2401 cgacatactt gagcgcctcc ggatcccgcg gacgatgctc cccgaggtcc tcccgtcgag
     2461 cggcgccttc ggcacgaccg accccgacgc gttcctcggc atcgaggccc cgatcgcggg
     2521 cgtcgccggc gatcagcagg cggccttggt cggccaggcg tgcttctccc ccggcgacgt
     2581 gaagaacacc tacggcaccg gctcgttcgt cctgctcaac accggcgagc gcgcgacggt
     2641 ctcgcgcgaa ggcctgctga cgacgatcgc ctactccgac gccgacggcg cgcgctacgc
     2701 gctcgagggg tcgatcttcg tcaccggcgc cgcgatccag tggctgcgcg acggcctcgg
     2761 catcctggag cgctcctccg acgccgagcc gctcgcccgc acggtgcccg acaccggcgg
     2821 cgtgtacttc gtccccgcgc tgacgggcct cggcgcgccg tggtgggatc cctacgcgcg
     2881 cggaacgatc gtcggcatca cgcgaggcac cactcgcgcc cacct
//
LOCUS       DATLGG010000228         4435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_93492, whole genome shotgun sequence.
ACCESSION   DATLGG010000228 DATLGG010000000
VERSION     DATLGG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93492"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VM638_06515"
     CDS             <1..548
                     /locus_tag="VM638_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVM12110.1"
                     /translation="SPLDPFALALAAVRRGRTLRFLAAAEFFERPIKGWFLRGLAQIP
                     VRRGTGGRSALDEAVRTLAAGAAVGIFPEGRVGEGMELARGRTGVARIALRSRAPIVP
                     VGIWGTQERWPRSGPRLGPPVRPGLAVVAAPAITPVGDPTSAADQRALIDLVMGAIGR
                     ALDQARKRAALTPPPGGSTRG"
     gene            complement(514..2001)
                     /locus_tag="VM638_06520"
     CDS             complement(514..2001)
                     /locus_tag="VM638_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12111.1"
                     /translation="MRRVLLVLAGFTLVVAGLPYAAAAAAGAPTTQRSSTTPPGSFEL
                     VGHEPLGNRGMNAALAIHGNYAYVGSRTDGKPGSQNLTNGGIMIVDISNPAAPRVVGQ
                     VTTPDEGNSGESSRELRVWRSQGILIVLRTNCSSQIHWCTSASPANNFRAYDISGANA
                     TSPKLIRQWTPGTHEFFLWEDPFDPNRALIFNTPASGSSFSIFDISPLRTGGAPVQLR
                     NGSHLMGGSAHSLSVSNDGKRAYFALLTGGFGMGDVSEFTSATPNPQYRPITGTRPTW
                     DGPGAHSAVKVWGKEWVAVFDEVYGTAARALGPHGCPWGWGRLIDIADPARPQVRAEY
                     KLAENQASYCQPVDPSPAASYSAHNPTFTPNVGLVTWHSGGLQAIDLSDPASPKQLAE
                     FRPTPLPFVVTEDPMLSAGLDKIVMWSYPIVRDGLIYVVDVRNGLYILRYKGPFQDEV
                     AEATFLEGNSNQGHALCYEPVLKPKEDPEEEDEYLIPEYCLPAEE"
     gene            complement(2092..2165)
                     /locus_tag="VM638_06525"
     tRNA            complement(2092..2165)
                     /locus_tag="VM638_06525"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2129..2131),aa:Ile,seq:cat)
     gene            2244..2444
                     /locus_tag="VM638_06530"
     CDS             2244..2444
                     /locus_tag="VM638_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12112.1"
                     /translation="MRTEDLVLEEQPYRSPDDEGMERGPEPGGGMEPGGGTGEEQEGE
                     EGGTSEEQEGEQGGQGEAGSPT"
     gene            complement(2457..2879)
                     /locus_tag="VM638_06535"
     CDS             complement(2457..2879)
                     /locus_tag="VM638_06535"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HVM12113.1"
                     /translation="MLRAFGRALLKRCPRCGTGRLFRRWFTMLERCPGCGLRFEREEG
                     YWVGALIVNTAATSVAFLVVLFGGMAAFWPEVPWVALTVTAALVNLVVPVVFYPWSKT
                     IWMAVDVALHRMDRQDRSVAGNGDATAQVLDRGVERER"
     gene            complement(2890..3966)
                     /locus_tag="VM638_06540"
     CDS             complement(2890..3966)
                     /locus_tag="VM638_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12114.1"
                     /translation="MTRRGVALRRSSRRQPAGLVVGVLLALAGIAGGSIGATWASFSS
                     EARNPENSFATATLAPPSDLTVRRTCEQSSTVGVRSSTSGSGSAGSLSIARPSGVASG
                     DVLLAQVAIRTGAATSVAAPEGWSSIRQDAAGTQVLQALYLKVAGASEPSSYTWTSAS
                     TGRAAGGIVAYTGVNQSNPVDAHAGGTGAVANMDAPSIRTETPNTRLVAAYALRQVTM
                     TLPSGMSSRWNLNSGGGSGSVTVGVADEAFVGPGATGDRRATSSNVGFDWVAQLVALR
                     PEATARASLSWTATPSSFAEGYRLQRWQGEALQVDRTLTPRTKTSESDGPVVTGESYT
                     YRLAAVSGDWRSAWTSVTFTPTAC"
     gene            complement(3963..>4435)
                     /locus_tag="VM638_06545"
     CDS             complement(3963..>4435)
                     /locus_tag="VM638_06545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVM12115.1"
                     /translation="RGFSRAMLALALLTFTVLGLMLASGLRPLVVRSGSMEPAIGTGD
                     VVFTRMVPPALLKRGEVVTFRDPSRGDDLVTHRVVEVRRAATSFGFVTRGDANTGVER
                     WSVERDGRIGRMALRVPRAGYVLAVFATAPVRAALLAAGFLVLSIVALRRIWAR"
BASE COUNT          680 a         1523 c         1616 g          616 t
ORIGIN      
        1 cgagcccgct cgatcctttc gcgctcgcgc tcgctgccgt ccgtcgtgga cggacgctgc
       61 gcttcctcgc ggccgcggag ttcttcgagc ggccgatcaa gggctggttc ctccgcgggc
      121 tcgcgcagat cccggtccgg cggggaaccg gtggacgttc ggcgctggac gaggccgtcc
      181 gaaccctcgc ggcgggcgcg gccgtcggga tcttccccga gggccgggtc ggagagggca
      241 tggaactcgc gcgcggcagg acgggggtgg cgaggatcgc gttgcgtagc cgagcgccga
      301 tcgttcccgt cgggatctgg gggacgcagg agcgctggcc ccggtccggc ccgagactgg
      361 ggccgcccgt gcgcccgggt ctggcggtcg tcgccgcgcc ggcgatcacg ccggtcggag
      421 atcccacctc agccgccgac caacgggccc tgatcgacct cgtgatgggc gcgatcggcc
      481 gcgcgttgga tcaggcccgg aagcgtgccg ctcttactcc tccgccggga ggcagtactc
      541 ggggatgagg tactcatcct cctcctccgg atcctctttc ggcttgagca ccggctcgta
      601 gcacagggcg tgtccctggt tcgagttccc ctcgaggaac gtcgcctcgg ccacctcgtc
      661 ctggaacggg cccttgtagc gcaggatgta gagcccgttg cggacatcca cgacgtagat
      721 caggccgtcc cgcacgatcg ggtagctcca catcacgatc ttgtcgaggc cggcggagag
      781 catcgggtcc tcggtgacga cgaacggcag cggggtggga cggaactcgg cgagctgctt
      841 cggtgaggcc ggatcgctca ggtcgatcgc ctggagcccg ccggagtgcc acgtcacgag
      901 gccgacgttc ggcgtgaagg tcgggttgtg ggcggagtac gacgccgcag ggctcgggtc
      961 gaccggctgg cagtacgagg cctggttctc ggcgagcttg tactccgcgc ggacctgcgg
     1021 acgcgctggg tcggcgatgt cgatcagtcg gccccagccc caggggcagc cgtgcggccc
     1081 gagggcgcgc gcggccgtgc cgtagacctc gtcgaagacg gcgacccact ccttccccca
     1141 gaccttgacg gcgctgtgcg cgcccggacc gtcccaggtg ggacgcgtcc ccgtgatcgg
     1201 tcggtactgt gggttcggcg tggcgctcgt gaactcggag acgtcgccca tcccgaaccc
     1261 gccggtgagc agtgcgaagt acgcgcgctt cccgtcgttg ctgaccgaga gggagtgcgc
     1321 gcttccaccc atgaggtgcg agccgttgcg cagctgaacc ggcgcgcctc cggtccggag
     1381 cggcgagatg tcgaagatcg agaagctcga gccgctcgcg ggggtgttga agatgagcgc
     1441 ccggttcggg tcgaaggggt cttcccacag gaagaactcg tgcgtccccg gggtccattg
     1501 acggatcagc ttcggcgagg tcgcgttcgc tcccgagatg tcgtaggcgc ggaagttgtt
     1561 cgccggcgag gccgaggtgc accagtggat ctgggagctg cagttcgtcc gcaggacgat
     1621 caggatgccc tgcgagcgcc agacccgcag ctcgcgggag gactcaccgc tgttcccctc
     1681 gtccggcgtg gtgacctggc ccaccacccg cggcgccgcg gggttcgaga tatccacgat
     1741 catgatcccg ccgttcgtca ggttctggct ccccggcttc ccgtcggtcc ggctgccgac
     1801 gtacgcgtag ttcccgtgga tcgccagagc ggcgttcatc ccccggttcc cgaggggctc
     1861 gtgtccgacg agctcgaacg acccgggagg ggtggtggac gagcgctggg tcgtgggagc
     1921 cccggccgcg gcggcggccg cgtacggcag acccgcgacg accagcgtga acccggcgag
     1981 caccagcaga accctgcgca tggatgacct cctcgatggg atgccgaagg gttcgccgcc
     2041 gagaccgccg ggtcctgccg aagaggttgt tcaactgcac aaaacgggcg gtggggcggc
     2101 caggactcga acctgggacc gacggattat gagtccgctg ctctgaccga ctgagctacc
     2161 gcccccgagc gagtatatcg accggtttgc gccggtcttc cgtcgggaaa gaacgtcgcc
     2221 cacgacggat cggaggtggg cggatgcgga cggaggatct cgtgctcgag gagcagccct
     2281 accgatcccc cgacgacgag gggatggagc gcgggccgga gccgggtggc ggcatggagc
     2341 cgggcggcgg cacgggcgaa gagcaggagg gcgaggaagg cggcacgagc gaagagcagg
     2401 agggcgagca gggcggccag ggcgaggccg gctcccccac ctgatccacg cgcggtctat
     2461 cgctcgcgtt cgacgccacg gtccagcacc tgggccgtgg cgtcgccgtt cccggccacc
     2521 gaacggtcct gacgatccat ccggtgcagg gcgacgtcga ccgccatcca gatcgtcttc
     2581 gaccacggat agaagacgac cggcaccacg aggttgacga gcgccgccgt taccgtaagc
     2641 gccacccacg gcacctcggg ccagaacgcg gccatgccgc cgaacaggac gacgaggaag
     2701 gcgaccgagg tggcggccgt gttcacgatc aacgcgccga cccaataccc ctcctcccgc
     2761 tcgaaccgca ggccgcaacc cggacagcgc tcgagcatcg tgaaccaacg ccggaacagg
     2821 cggcccgtgc cacaccgagg gcaccgcttg agaagtgcgc ggccgaacgc gcggagcatg
     2881 acaggcacgc tagcaggcgg tgggagtgaa ggtgacgctg gtccaggctg accgccagtc
     2941 gccggatacc gccgcgagac gatacgtgta ggactccccg gtcaccaccg gtccgtcgga
     3001 ctccgaggtc ttggtcctcg gcgtcagcgt gcggtcgacc tgaagagctt ctccctgcca
     3061 ccgctgaagc cggtagccct ccgcgaagct cgagggtgtc gcggtccagc tgagcgatgc
     3121 gcgcgccgtc gcttcgggcc gcagcgcgac cagctgggcc acccagtcga agcccacgtt
     3181 gctcgaggtc gcccgtcggt cccccgtggc gcccggaccc acgaaggcct cgtccgcgac
     3241 cccaaccgtc acgctgccgc tgccgcctcc cgaattgagg ttccaacgcg aggacatccc
     3301 cgaggggagc gtcatcgtta cctgacggag cgcgtaggcg gcgacgagcc gggtgttggg
     3361 ggtctccgtg cggatcgagg gcgcgtccat gttcgcgacc gcgccggtcc cgccggcatg
     3421 cgcatcgacc gggttcgact ggttgacccc cgtgtaggcg acgatcccgc ctgcggcccg
     3481 gccggtcgag gcagaggtcc aggtgtagct cgacggctcg gaagcgccgg cgaccttgag
     3541 gtagagcgct tgcaggacct gcgtccccgc ggcgtcctgt cggatggagg accagccctc
     3601 gggcgcggcg acggacgtcg cggcccctgt cctgatcgcg acctgagcga ggagaacgtc
     3661 gcccgacgcc acccccgagg gccgggcgat cgagagcgac ccggccgagc cgctgccgga
     3721 ggtcgaggag cggacgccca ccgtggagga ctgctcgcac gtgcggcgga cggtgaggtc
     3781 ggagggagga gccagcgtcg ccgtggcgaa gctgttctcc gggttgcgcg cctcgctcga
     3841 gaaggacgcc caggtggctc cgatgctgcc gccggcgatc ccagccagcg cgaggaggac
     3901 ccccacgacg agtccggccg gctggcgtcg gctcgagcgt cgaagggcga ctcccctgcg
     3961 ggtcatcgcg cccagatcct ccgcagggcg acgatcgaga gaacgaggaa gccggccgcg
     4021 aggagcgccg cgcgcaccgg cgcggtggcg aacacggcga gcacgtaccc tgcacgcgga
     4081 acgcggagcg ccatacgccc gatgcgcccg tcgcgttcca ccgaccagcg ttcgactccc
     4141 gtgttggcgt ccccacgtgt cacgaagccg aacgaggtcg ccgcgcgccg gacctcgacg
     4201 acccggtggg tcacaaggtc gtccccgcgg gaggggtccc ggaacgtgac cacctccccg
     4261 cgcttcagca gcgccggcgg caccatcctg gtgaagacca cgtcaccggt tccgatggcc
     4321 ggctccatgc tgccgctccg gacgacgagc ggtcggaggc cggatgccag catgagaccc
     4381 aacaccgtga acgtgaggag cgcgagcgcg agcatggcgc gggagaaccc gcgcc
//
LOCUS       DATLGG010000229         6723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_47094, whole genome shotgun sequence.
ACCESSION   DATLGG010000229 DATLGG010000000
VERSION     DATLGG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47094"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /gene="dnaE"
                     /locus_tag="VM638_06550"
     CDS             complement(<1..1722)
                     /gene="dnaE"
                     /locus_tag="VM638_06550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVM12116.1"
                     /translation="MATKSFVHLHTHSEFSLLDGASRIDAMFESAARMGMPALAVTDH
                     GTMFGALDFYRAGVKAGVKPIIGVEAYVAPGSRFDRNPGESEEKYRHLTILARNEQGY
                     RNLLKLVTAAHLEGFYTRPRIDKELLAEHREGLIGLSGCLASEVCRQILAGQERKALE
                     AAARFRDIFGPDAFYIELQDHGLEDQHRVNPVLLRIARDLGIPLVATNDTHYTHKEDA
                     RAHDVLLCIQQHGKVLADTNRLRFDTEEFFVKSAEQMRHLFRDVPEACDATLEIAGSV
                     EPIPDLDAAVEHRSPLRFPRFPAPEGRSLEAHLRDLVLDGARRRYGEPTPEVLDRVEH
                     ELSIIASMGFAGYFLIVWDLIRFARERGIRVGPGRGSAAGSVVSYCLGITDIDPLRYG
                     LIFERFLNPERKQMPDIDMDFDERRRDEVIRYVAQKYGSDHVAQIVTFQTIKGKQGIR
                     DSARVLGYPASVGDRLCKMYPAAVLGRDAPIDKALKDSTELREAYEKEPEAREIIDSA
                     RALEGLRREDSVHAAGVVIGDAPLVNYLPLKLSKDSRDDSKRIVTQFDMHHVEELGLL
                     KMDFLGLR"
     gene            1847..2965
                     /locus_tag="VM638_06555"
     CDS             1847..2965
                     /locus_tag="VM638_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVM12117.1"
                     /translation="MKLSYSSLNTYETCPAKYRFQYEDRLSASRSPALAFGDSLHRAL
                     QRFHARPVPVAPSLEELWQFLEDEWVSDGYRDEVEERPYRDHARQVLAEYHRQNAHAY
                     RIPAALEFRFEIEVEGVTLTGVIDRLDRLPGGGYEIIDYKTNRRLPPRKVVERDLQLS
                     IYALAAREVWGIEPERLTLYYLLPGERFTTTRSPADLDALRGRLATAAERIEAGMFEP
                     RENRLCGWCDFRPLCPLFRHLYRKDEAPRIAEVVDEWVALKREDRERTRRLEELGAMI
                     RAYAEEHGLGQLFGEDGAVKIVERIETAPDPEAVRRALEPLGLYDSVISVDPRKLQEL
                     FERRVLPPDAEESLLSGRDEVRKVKALHLRDADRAGRR"
     gene            complement(3119..6091)
                     /locus_tag="VM638_06560"
     CDS             complement(3119..6091)
                     /locus_tag="VM638_06560"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HVM12118.1"
                     /translation="MSDKLPADGWAFGSRVDRGRERRSRTRTMLLAFALVATVLGTVL
                     PALGQEEPVEQGEVAEVVEESVVEEAAEPSSPGSSSSGAESPAEGSSGEAEQAPAPAA
                     PKNNPWIKSDKEDYVPGELVTLTGGDWLPGENVNIFVDDDGIREQKWSRTVDVTADSD
                     GQIEDSFSLPDWFVADYLVVATADSGTARTTFTDGNLRFKVNHSQASFDADWSRHNGT
                     NCSNGQRFDQGTTTIGHTNSTLVLAAEATQSVKVTALATVGTRTFSGWTGPGGWSSSS
                     LTICLPGFAGSGIQDYMANYVTTAAPTTTSVGSITASASVFGGTTNLSATVSPANSPG
                     SVSFFVNGSSTPAAGTVSYAQSTGIATLSGLNHGLNASSTAYSVRAVFTSSNTGTHTS
                     SEGTNASALTVSKANQTLSFTAGTVPASALFGGTFTPGATATSGLSPAISVSGGCSIL
                     SGVVTMTSGTTACLVKASQAGDTNYNPAPEISQSVTAGKAAATIHLSGLSKTYTGSPQ
                     AATATTTPAGLSGVSITYDGSPSTPTNAGSYLVSASLNHDDYAATPATGTLVIAPKSV
                     TVTPDSDQSKVFGTALDPVLTFTLSEAVATTGALARAEGENVGTYAINLGSLASSSTN
                     HTLALSATTVSFSITPKPVTITPSSGQGKVYGSPDPVLTFSNSEGLGDVFSGALSRAA
                     GDDVGDYAIGLGSLSAGSNYSLSLAEGSYSFSITPKTVTVLPTANQSKVYGQADPAAF
                     AYTLSESVPVNGALDRVPGEDVGTYAINLGSLVTTSGNHTLALSATVVSFSITPKPVT
                     VTPDPGQSKIFGASDPALTYALSEALAVTGSLGRAPGETVGSYAFDLGTLATSSTNHV
                     LVLAHPAGSFTITGWLIAAAPFKQPVDNVPTWNVVKAGSAVPVKFSLGGDKGTNIFSA
                     GFPKPESIACDSAAQLDGIETTVAATANSLTYDSVSDTYTYVWKTDKAFATGTGCRQL
                     VMRFLDGTIRRANFKFTK"
     gene            complement(6306..>6723)
                     /locus_tag="VM638_06565"
     CDS             complement(6306..>6723)
                     /locus_tag="VM638_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM12119.1"
                     /translation="ADSGKVVISSGTGTCSITAHKAADDNYSSTSSAPLELDLVAGVV
                     IIRCPGISAAGNHIVGSAGANTLRGTPGRDIICGLGGNDTIYGKGGNDILIGGAGKDT
                     LYGGAGKDSLRGGAGRDSCFGGGDLDSFRSCETRRQ"
BASE COUNT          999 a         2233 c         2333 g         1158 t
ORIGIN      
        1 gcggagaccg aggaagtcca tcttcagcag gccgagctct tcgacgtggt gcatgtcgaa
       61 ctgcgtcacg atccgcttcg agtcgtcgcg ggagtccttc gagagcttga gcggcaggta
      121 gttcacgagc ggcgcgtccc cgatgacgac gccggcggcg tggacggaat cctcacggcg
      181 gaggccctcg agcgcgcggg cggagtcgat gatctcccgg gcctcgggct ccttctcgta
      241 cgcctcccgc agctccgtgc tgtccttcag tgccttgtcg atcggcgcgt cgcgtccgag
      301 gacggccgcc gggtacatct tgcagaggcg gtcgccgacg ctggcggggt acccgaggac
      361 gcgagccgag tcgcggatcc cctgcttccc cttgatcgtc tggaacgtga cgatctgcgc
      421 cacgtggtcg ctcccgtact tctgcgcgac gtagcggatc acctcgtcgc gccggcgctc
      481 gtcgaagtcc atgtcgatgt caggcatctg cttgcgctcg gggttgagga accgctcgaa
      541 gatcagcccg tagcgcagcg ggtcgatgtc ggtgatcccg aggcagtacg agacgacgga
      601 gccggcggcc gacccgcggc ccgggccgac gcggatcccg cgctcgcgcg cgaaccggat
      661 caggtcccag acgatgagga agtacccggc gaaccccatc gaggcgatga tcgacagctc
      721 gtgctcgacc cggtccagca cctccggcgt cggctcgccg tagcgccggc gcgcgccgtc
      781 gaggacgagg tcccgcaggt gcgcctcgag gctcctgccc tcgggggcgg ggaaccgcgg
      841 gaagcgaagc ggggatcggt gctcgaccgc ggcgtcgagg tcggggatcg gctcgacgga
      901 cccggcgatc tcgagggtgg cgtcgcaggc ctcggggaca tcgcggaaca gatgccgcat
      961 ctgctcggcc gacttcacga agaactcctc ggtgtcgaac cggaggcggt tcgtgtcggc
     1021 caggaccttg ccgtgctgtt ggatgcagag caggacgtcg tgcgcccggg catcctcctt
     1081 gtgggtgtag tgggtgtcgt tcgtcgcgac gagggggatc ccgaggtccc gcgcgatccg
     1141 caggagcacc gggttcacac ggtgctggtc ctcgaggccg tggtcctgga gctcgatgta
     1201 gaaggcgtcc gggccgaaga tgtcgcggaa ccgcgccgcg gcctcgagcg ccttccgctc
     1261 ctggcccgcg aggatctgcc ggcagacctc cgaggcgagg cagcccgaga gcccgatgag
     1321 cccttcacgg tgctcggcca gcagctcctt gtcgatgcgc ggccgcgtgt agaacccctc
     1381 gaggtgagcc gcggtcacga gcttcaggag gttgcgatag ccctgctcgt tgcgggcgag
     1441 gatcgtcagg tggcggtact tctcctcgct ctccccgggg ttgcggtcga acctcgagcc
     1501 cggagcgacg taggcctcca ccccgatgat cggtttgacc cccgccttga cgccggcacg
     1561 gtagaagtcg agggcgccga acatcgtgcc gtggtcggtg accgcgagcg ccggcatccc
     1621 catgcgggcg gcggactcga acatcgcatc gatgcggctg gccccgtcga ggagcgagaa
     1681 ctcgctgtgc gtgtgaaggt ggacgaagct cttcgttgcc atgcctcggt tgcctgcgga
     1741 tggcggggaa gcccttgaac cggaccggag ccgagtatag gctgggtccc gacggaatta
     1801 caagtatgtc attctatcca caggttttcc acacccgacg ggaccgatga agctctccta
     1861 ctcctctctc aacacctacg agacgtgtcc ggcgaagtac cgcttccagt acgaggatcg
     1921 gctgtcggcc tcccgttccc ccgcccttgc cttcggcgac tcgctccacc gcgcgctcca
     1981 acggttccac gcacgaccgg tcccggtggc gccgtcgctc gaggagctct ggcagttcct
     2041 cgaggacgag tgggtctccg acggctaccg cgacgaggtg gaggagcgtc cgtaccgtga
     2101 ccacgcgcga caggtcctcg ccgagtacca ccggcagaac gcacacgcct accggatccc
     2161 cgccgccctc gagttccgct tcgagatcga ggtcgagggg gtgacgctca cgggggtcat
     2221 cgaccggctc gaccgcctgc ccgggggagg ctacgagatc atcgactaca agacgaaccg
     2281 tcggctcccc ccgaggaagg tcgtcgagcg ggacctccag ctctcgatct acgcgctcgc
     2341 cgctcgtgag gtgtggggga tcgagccgga gcggctcacc ctctactacc tccttcccgg
     2401 ggagcggttc accacgacgc gctcccccgc cgacctcgac gccctccggg ggcggctcgc
     2461 cacggccgcc gagcgcatcg aggcggggat gttcgagccc cgcgagaacc gcctgtgcgg
     2521 ctggtgcgac ttccggcccc tctgcccgct gttccggcac ctctaccgga aggatgaagc
     2581 ccctcggatc gcggaggtcg tcgacgagtg ggtcgccctg aaacgcgagg accgggagcg
     2641 gacccgccgc ctcgaggagc tgggagcgat gatccgcgcc tacgccgagg agcacgggct
     2701 ggggcagctc ttcggggagg acggcgcggt gaagatcgtg gaacggatcg agacggcgcc
     2761 agacccggag gcggtgcgcc gagccctcga gcccctggga ctgtacgact cggtgatctc
     2821 cgtcgacccg cggaagctgc aggagctctt cgagcgccgg gtgctccctc ccgatgcgga
     2881 ggagagcctg ctgtcaggcc gcgatgaggt ccggaaggtg aaggcgctcc acctgcgcga
     2941 cgccgatcgc gcaggccgcc gctagcccgt tcgcagcacg tggaccaggg tcaacaggac
     3001 agccgccgtc acagccatgg tcgtcagcag gaacagcgcg cgtccggtca cgggatcctc
     3061 tctctgaagg gtgcagagag gggcggcctg acggccgccc ctctctcgtg ctccctccct
     3121 acttggtgaa cttgaagttc gcgcgcctga tggtgccgtc gaggaacctc atcacgagct
     3181 gacggcagcc cgtgccggtg gcgaaggcct tgtccgtctt ccagacgtac gtgtaggtgt
     3241 cggacaccga gtcgtaggtc aggctgttgg ccgttgccgc aaccgtggtc tcgataccgt
     3301 cgagctgtgc cgccgagtcg cacgcgatgc tctcaggctt ggggaagccg gcggagaaga
     3361 tgttcgtccc cttgtcaccg ccgaggctga acttcaccgg gaccgcgctg ccagccttga
     3421 cgacgttcca ggtcggcacg ttgtccaccg gctgcttgaa cggcgctgcg gcgatcagcc
     3481 atcccgtgat cgtgaagctc ccggcggggt gcgccagaac gagcacgtgg ttggtgctcg
     3541 aggtggccaa cgtgcccaga tcgaaggcgt agcttcccac cgtctctcca ggggcgcgac
     3601 ccagggagcc cgtaaccgcc agcgcctcgc tcagcgcata ggtcagggcg ggatccgagg
     3661 caccgaagat cttgctctgg ccagggtctg gcgtaacggt gacgggcttc ggcgtgatcg
     3721 agaagctcac gacggtcgcc gacagcgcca gcgtgtggtt cccgctcgtc gtcacgaggc
     3781 tgcccaggtt gatcgcgtag gtcccaacgt cttctcccgg gacacgatcg agagctccgt
     3841 tcaccggaac cgactcgctg agcgtgtacg cgaacgccgc ggggtccgct tggccgtaga
     3901 ccttgctctg gttcgccgtc ggcagcaccg tcacggtctt cggggtgatc gagaagctgt
     3961 agctcccttc ggcaaggctc aggctgtagt tggacccagc cgaaagactc cccaggccga
     4021 tcgcgtagtc accgacgtcg tccccggccg cgcggctgag agcgcccgag aacacgtcgc
     4081 cgagcccctc gctgttggag aaggtgagca ccgggtcggg gctgccgtac accttgccct
     4141 gacctgagct cggagtgatc gtgaccggct tcggggtgat cgagaagctc acggtcgtcg
     4201 ccgacagcgc cagcgtgtgg ttggtgctcg agctcgcgag gctgccgagg ttgatcgcgt
     4261 aggtacccac gttctccccc tcggcgcggg cgagggctcc ggtcgtcgcg accgcctcgc
     4321 tcagcgtgaa ggtgagcacc gggtcgagtg ccgtaccgaa caccttcgac tggtcactgt
     4381 ccggcgtcac cgtgacgctc ttcggggcga tcacgagggt gcctgttgcc ggcgtcgccg
     4441 cgtagtcgtc gtggttcagg gacgcggaga ccaggtagct gccggcgttg gtcggtgtcg
     4501 agggggaccc gtcgtacgtg atcgatacgc cggacagtcc ggcaggggtg gtcgttgccg
     4561 tcgccgcctg cggtgacccg gtgtaggtct tgctcaggcc ggagagatgg atcgtggccg
     4621 cggccttgcc ggccgtgacc gactgggaga tctccggcgc cgggttgtag ttcgtgtcgc
     4681 cggcctggct ggccttcacg agacaggccg tggtgccgct cgtcatcgtg acgacgcctg
     4741 acaggatcga gcagccgcca ctcacggaga tcgccggaga gagacccgac gtggccgtcg
     4801 cgcccggggt gaacgtgccg ccgaacaggg cggacgccgg cacggtcccc gcagtgaacg
     4861 agagggtctg gttggccttg ctcacggtca acgcggaggc gttcgttccc tcgctgctcg
     4921 tgtgcgtccc ggtgttgctc gaggtgaaga ccgcccggac gctgtaggcc gtgctgctgg
     4981 cgttcaggcc gtggttgagc cccgagagcg tggcgatccc cgtcgactgg gcgtaggaga
     5041 cggtccccgc cgcgggagtg ctgctgccgt tcacgaagaa cgagacgctc cccggcgagt
     5101 tggccggcga gacggtcgcc gagaggttcg tcgtcccccc gaagaccgag gcggaggcgg
     5161 tgatgctccc gacgctcgtg gtggtcgggg cagccgtggt cacgtagttg gccatgtagt
     5221 cctggatccc gcttccagcg aacccaggga gacagatcgt gagactcgac gacgaccacc
     5281 cgcccggccc ggtccatccg ctgaacgttc tggttcccac ggttgccagc gccgtgacct
     5341 tcaccgactg cgtcgcctcg gcggccagga cgagggtgct gttggtgtgc ccgatcgtgg
     5401 tggtgccctg gtcgaacctc tgcccgttcg aacagttcgt gccgttgtga cgggaccagt
     5461 ccgcgtcgaa agacgcctgg gagtgattca ccttgaagcg caggttcccg tcggtgaagg
     5521 tggttcgtgc cgtgccggaa tcggcggtcg ccacgaccag ataatcggcg acgaaccagt
     5581 ccgggaggct gaaggaatcc tcgatctgcc cgtcgctgtc ggcagtcacg tcgacggtgc
     5641 gggaccactt ctgctcgcgg atgccgtcgt cgtcaacgaa gatgttgacg ttctcgccgg
     5701 ggagccagtc cccgcccgtg agcgtgacga gctcgccggg aacgtagtcc tccttgtcgc
     5761 tcttgatcca ggggttgttc ttgggtgccg ccggagcggg cgcctgctcg gcctctccac
     5821 tcgatccttc cgcgggagac tcggctcctg agctcgaaga tcccggagag gagggttcgg
     5881 ccgcctcttc cacgacgctt tcctctacga cctcggcgac ctcgccctgc tcgacgggct
     5941 cttcctgccc gagggcaggc aggaccgtgc cgaggaccgt cgcgaccagc gcgaaggcca
     6001 ggagcatcgt tcgtgtgcgg ctgcgacgct cgcgtccgcg atcaacgcgc gatccgaacg
     6061 cccatccatc cgcagggagc ttgtcggaca tgtgttcctc ctcttcctcg ggctgcggcc
     6121 gacgggcgtc gtgccgctgt cggtcgattg tccgagggcc gaggggtcca cggcatcgcc
     6181 aggattggcc gaaacttgat actcaaggtt gaccgttccg ggtggggcgt ccctgccccc
     6241 agcacggatc gggccccccc aaggttctgg gagggcccga gctcggtcgt gcgggtcttc
     6301 gttgactact ggcgacgcgt ttcacagctc ctgaagctgt ccaggtcacc gccaccgaag
     6361 cagctatccc gtcctgcgcc gccccggagg gagtccttcc cggcgccgcc gtacagcgtg
     6421 tccttaccgg cgccaccgat caggatgtcg ttgccgccct tcccgtagat ggtgtcgttg
     6481 ccccccagcc cgcagatgat gtctcggcca ggcgttcctc ggagggtgtt tgctcctgcg
     6541 ctgccgacga tgtggttccc agcggcagat atgcccggac acctgatgat caccactcca
     6601 gccacgaggt ccagctcgag aggggccgag ctcgtgctgc tgtagttgtc gtcagccgcc
     6661 ttgtgagcgg tgatggaaca cgtgcccgtg ccagagctga tcacgacctt gcccgaatcg
     6721 gct
//
LOCUS       DATLGG010000230         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_196770, whole genome shotgun sequence.
ACCESSION   DATLGG010000230 DATLGG010000000
VERSION     DATLGG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196770"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VM638_06570"
     CDS             complement(<1..822)
                     /locus_tag="VM638_06570"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVM12120.1"
                     /translation="MRDRIVIANAGGFWGDDPTAAGRQVRGGKIDYLVMDYLAEVTMA
                     ILQKQKAKDPKAGYAKDFLVQLREVLPACVEKGITIIANAGGVNPLACRDAVDALAKE
                     LGVADKVKVGVIIGDDLMGDLDRLLEAGEPLKHMDTGRPLSEVRSQVVSANAYLGAAP
                     VVKALSMGANVIIAGRVTDTAVTLGPMIHEFGWSFDDWDKIASGVIAGHIIECGTQCT
                     GGNFTDWEKVPSYQDLGFPLIEANADGTFVVTKHPKTGGLVSVHTVTEQLLYEMGT"
     gene            complement(937..1281)
                     /locus_tag="VM638_06575"
     CDS             complement(937..1281)
                     /locus_tag="VM638_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12121.1"
                     /translation="MSIHYLEIVSEDADSLIRLHERMHGLSFGEPDPDLGHARVAMRP
                     DGTLIGIRKPLAAHEGATMRTYLEVDDIERAVKAAADAGALVAYGPTRQGVRGTFAIV
                     IQGNVQHGLWQR"
     gene            complement(1368..1751)
                     /locus_tag="VM638_06580"
     CDS             complement(1368..1751)
                     /locus_tag="VM638_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12122.1"
                     /translation="MIRSILAVVAGYLAMFIGVSIFFTFVVFFAWGGKLPDPATYTTP
                     PSWLLYAELAWVPLVSVAGGWVCAFVARSRPMRHAAALVAIMAPLTAVSIVGEWGMKP
                     VWSLLGVPVLGMAGVLLGARLYRAV"
     gene            complement(1818..>2363)
                     /locus_tag="VM638_06585"
     CDS             complement(1818..>2363)
                     /locus_tag="VM638_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM12123.1"
                     /translation="LYARYADRVASFFGRKTSADVPDLVQRTFLKLFEAAKAGVEIGR
                     VDAMLFTIARNELYDSFRRARREGDPFDPDAMTLEDQATRASQLLARREEQTLLLQAL
                     RRLPLDDQLALELYYWEELPMEEVARVLGATKSAAINRVHRARGALKAVLERLEARPE
                     LVRETISDLEGWAKSLKDLAK"
BASE COUNT          362 a          809 c          824 g          368 t
ORIGIN      
        1 ggtgcccatc tcgtagagga gctgctcggt gacggtgtgc accgaaacga gcccgcccgt
       61 cttcgggtgc ttcgtgacga cgaacgtgcc gtcggcgttc gcttcgatca gcgggaagcc
      121 gaggtcctgg tagctcggga ccttctccca gtcggtgaag ttgccgcccg tgcactgcgt
      181 gccgcactcg atgatgtgtc cggcgatgac gccggaggcg atcttgtccc agtcgtcgaa
      241 cgaccagccg aactcgtgga tcatcggccc gagcgtcacc gcggtgtcgg tcacgcggcc
      301 ggcgatgatc acgttcgccc ccatcgagag cgccttcacc accggcgccg cgcccaggta
      361 ggcgttggcg gacacgacct gcgagcggac ctcggagagc ggacggccgg tgtccatgtg
      421 cttcaggggc tcgccggcct cgaggaggcg gtcgaggtcc cccatcaggt cgtcgccgat
      481 gatcacgccg accttcacct tgtcggcgac gccgagctcc ttcgccagcg cgtcgaccgc
      541 gtcgcggcac gcgagcgggt tcacgccgcc tgcgttcgcg atgatcgtga tgcccttctc
      601 cacgcaggcg gggaggactt cgcggagctg cacgaggaag tccttcgcgt agcccgcctt
      661 cgggtccttc gccttctgct tctggaggat ggccatggtg acctcggcga ggtagtccat
      721 gaccaggtag tcgatctttc cgccgcgcac ctggcggccg gcggcggtgg ggtcgtcgcc
      781 ccagaagccg ccggcgttgg caatgacgat gcgatcgcgc atgcgcggga acctactacg
      841 ccgattcttc ggcgtgccag atgatcggcg agggagcgat tcgagccctg aagcggactc
      901 cgcgagcagg acgggggccc gcgctcgcgg agacggtcag cgctgccaga ggccgtgttg
      961 cacgttcccc tggatcacga tcgcgaacgt gcctcgcacg ccctgccgcg tcgggccgta
     1021 ggcgacgagg gcgccggcgt ccgcggcggc cttcacggcg cgctcgatgt cgtccacctc
     1081 gagataggtc cgcatcgtcg cgccctcgtg cgcggcgagg ggcttccgga tgccgatcag
     1141 cgtgccgtcg ggccgcatcg cgacccgcgc gtgtccgaga tcggggtcgg gttctccgaa
     1201 cgacaggccg tgcatgcgtt cgtgaagccg gatgagcgaa tcggcgtcct cgctgacgat
     1261 ctcgaggtag tggatggaca tcgggcgact cccagcggct cgggctctcg tggcgcggcg
     1321 ggctccgccg gtttccggat cccgccgcga gaggtacgag acgtatccta gaccgcccgg
     1381 tagaggcgcg cgcccagcag gacaccggcc atgcccagga ccggcacccc gaggagtgac
     1441 cagacgggct tcatgcccca ttcgcccacg atcgacaccg ccgtgagcgg cgccatgatc
     1501 gcgacgagcg ccgccgcatg ccgcatcgga cgcgaccggg ccacgaacgc gcagacccag
     1561 ccgccggcga ccgagacgag cggaacccag gcgagctccg catagagcag ccagctcgga
     1621 ggcgtggtgt aggtcgccgg atcggggagc ttcccgcccc acgcgaagaa cacgacgaag
     1681 gtgaagaaga tcgagacgcc gatgaacatg gcgaggtagc cggcgacgac ggcgaggatc
     1741 gagcggatca tgacgggttc ctttcgtttg cctctctcgc catcaaggac gtccgtccct
     1801 ccgcgcgatc gcggaaatta tttggccagg tccttcaagg acttggccca cccctccagg
     1861 tcggagatcg tctcgcggac gagctcgggg cgcgcttcga gccgttccag gacggccttg
     1921 agcgccccgc gcgcgcggtg gacccggttg atcgccgcgc tcttcgtcgc gccgaggacg
     1981 cgggcgacct cctccatcgg aagctcctcc cagtagtaca gctcgagcgc gagctgatcg
     2041 tcgagcggca gccgccgcag agcctggagg agcagcgtct gctcttcgcg ccgcgcgagg
     2101 agctgcgacg cgcgcgtggc ctggtcctcg agggtcatcg cgtccggatc gaacgggtcc
     2161 ccctcgcggc gcgcccgccg gaacgagtcg tagagctcgt tgcgcgcgat cgtgaagagc
     2221 atcgcgtcca cccggccgat ctccacgccc gccttcgcgg cctcgaagag cttcaggaac
     2281 gtccgctgga cgagatcggg gacgtcggcc gaagtcttcc gtccgaagaa gctcgcgacc
     2341 cggtcggcgt agcgggcata cag
//
LOCUS       DATLGG010000231         6987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_30976, whole genome shotgun sequence.
ACCESSION   DATLGG010000231 DATLGG010000000
VERSION     DATLGG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30976"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VM638_06590"
     CDS             <1..505
                     /locus_tag="VM638_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVM12124.1"
                     /translation="TGRRVPWPSREELREARKVLDELGLGAVAGRHIRELSGGQQQRV
                     FIARALLGHPRLLLLDEPTSGVDVHTRHDILHLLHELHHDGLAIVVTTHDLNGVAAHL
                     PEIVCLNREVVAVGPPRRVLTPEVLERTYGAPMEVLHHGGMPVVVDHPEDVEVLHGEE
                     HHHEHPA"
     gene            489..1355
                     /locus_tag="VM638_06595"
     CDS             489..1355
                     /locus_tag="VM638_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HVM12125.1"
                     /translation="MSILLEPFAYGFFVSGVVVATLAGVLCGLVGVYVVLRGMSYIGH
                     GLSHAIFGGAVASFVAKVNFYVGAAAWGFVAALLVNRVARRRTIGADAAIGVVTTASF
                     AVGLALISRFRSFTRNFEAALFGNVLGVRLQDALVVGGVLLVAAAFVFFAYRPLLFTT
                     FDPEVAEASGVPTGRIDAGFALLLAATIVATMNILGVLLIAAALVIPAVVARLLTDSF
                     GRMLWLSMLVGGLSGFAGMYLSWYLNVSSGATIVLTQAAFFMVAFALTGLRRRHGIAP
                     RPAVPPGTQSGW"
     gene            complement(1862..2290)
                     /locus_tag="VM638_06600"
     CDS             complement(1862..2290)
                     /locus_tag="VM638_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVM12126.1"
                     /translation="MREVEEIIGRLRARGHRLTPQREAIIEEVLRTDGHITPQAVARR
                     VRRRMPAVNASTVYRTLATLEDLGVVQHAHRESGAEYHRAGEGDHVHLTCSRCGAEDD
                     LSMAEAGSLRDLIRRHRGFDPDLTHFAISGLCARCSAETR"
     gene            complement(2323..3627)
                     /locus_tag="VM638_06605"
     CDS             complement(2323..3627)
                     /locus_tag="VM638_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12127.1"
                     /translation="MRVEAITMHRVAVLLTALLLWVPAAPAPARSPVEPFRGLGAWVD
                     IFDDAAWAAPEATVADLRARGVETLYLQTTNPKEGGPLRFPGGTSRFLDAAHANGMRV
                     VGWYVPDFHRPRRDLRWSLAAIRFRSATGRSFDGFALDIEITKVRDHAERSRRLVRLS
                     HDLRAAVGRSYPLGAIIPSILVPSKDGGRIHPGYWSDFPYAGIAPHYDAFLPMAYWTY
                     RTSGERGTHAFIARSIQLIRQGTGRPDVPIHVIGGIANEVSAADMRGFDRAVREGGAI
                     GASMYDAATSGRMVWGALAALKARLPRPADPAIAPAAPAPPPTVVALPPPPGQWSVDP
                     TATQLEGGVRVTFAFPSGGAAGWANVRAYDVSPGEIRVVLNGKPVGILRATQPGAWGG
                     VQRVPLRGLGPRNRLTFESALNPPGTDPWGVQILGTAALTPL"
     gene            complement(3645..4919)
                     /locus_tag="VM638_06610"
     CDS             complement(3645..4919)
                     /locus_tag="VM638_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12128.1"
                     /translation="MASPPPGGSTLRTRSILIVAALAASLLPASPAQAAPSLAAYRGL
                     GAWIDIYDPTAWDQPGRKVRRLAARGVRTLYLQSNNYHQPGDIRFPAATGRFVDAAHR
                     HGMKIVAWYLPGLKDLYRDQRRAMAAIRFRSPEGRRFHSFALDIEATIVKDIDARNRR
                     LLRLSDFLRARVGSSYPMGAIIPSPRGMQLAPGYWPRFPYRALAERYDVFVPMTYYSW
                     RASGAAEVTDYMRRSIRILRSRTEDPGVPIHAIGGLADGSSRSETRAYVRSVREHGLL
                     GGSLYDDSLFGAEDWEELARVQPNPPRQSPPMPLRFGEHTDGYGRITGGDRTHPDEVV
                     YRHGPLSSDQVLSFEGFDIGAGEVEVQVNWTRLVVPLAAGPPEDWTPARQTILIPASA
                     LRPDAKNVIAFVPRIRHPWTTWGVRGVTLAPA"
     gene            4957..5736
                     /locus_tag="VM638_06615"
     CDS             4957..5736
                     /locus_tag="VM638_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVM12129.1"
                     /translation="MGELVRLEVEEGVGTIRLDRPPMNAINAELVTDLRAAALEAAER
                     EDVRAVVLYGGEKVFAAGADVKMMAEMGPAEIRPVIRMMQETFTLVEEIPKVTIAALT
                     GYALGGGCELALAADLRFAAEDARIGQPEILLGIIPGAGGTQRLPRLVGVSRAKDLIY
                     SGRHLRAQEALEIGLVDAVHPSGEVYGAAAEQARRYAAGPTQALRAAKIAIDRGVRTD
                     IGTGLAIEREVFCDLFFTEDQREGMRSFVEQGPGKATFRGR"
     gene            5866..6063
                     /locus_tag="VM638_06620"
     CDS             5866..6063
                     /locus_tag="VM638_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12130.1"
                     /translation="MSEYIKGSRPRQLPPASRTYGGGRVCAEQGCETRISIYNKATHC
                     WAHAPLRYPVSRGERRKKTAA"
     gene            6112..6780
                     /locus_tag="VM638_06625"
     CDS             6112..6780
                     /locus_tag="VM638_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVM12131.1"
                     /translation="MPPSGIAPERRPPRAAIGLLLAGLAVFGLLAVLVLLAEPALLAI
                     DRPLSEALRADRGDVLAEVMRGISLLGSRAVIGGILLALTLWAIVTRECQRTVGIVLV
                     VFAVAMVLEWTLKAGIGRLRPEEAFALTRVTTAAFPSGHALGSAAVYAILAVALGGGA
                     RGHALVWLLIAAIGVSRVVLGVHWPSDVLGGIALGAAIAAGGVLALRGHRLDPKRCPG
                     GHGT"
     gene            complement(6777..>6987)
                     /locus_tag="VM638_06630"
     CDS             complement(6777..>6987)
                     /locus_tag="VM638_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12132.1"
                     /translation="RSGEGPRVRLERLAGDATAVFRRAGGGWTEIPPEEGLERGADAC
                     VEALLDETVFVALRVFLGAEGCGPV"
BASE COUNT          937 a         2453 c         2581 g         1016 t
ORIGIN      
        1 aacgggccgg cgtgttccct ggccgtcgcg tgaggagctc cgcgaggctc ggaaggtcct
       61 cgacgagctc gggctcggag cggtcgccgg ccggcacatc cgcgagctct cgggcggcca
      121 gcagcagcgc gtcttcatcg cccgtgcgct cctcggccat ccgcgtctgc tgctgctcga
      181 cgagccgacg agcggcgtcg acgtgcacac gcgccacgac atcctccatc tcctccacga
      241 gctgcaccac gacgggctgg cgatcgtcgt cacgacgcac gacctcaacg gcgtcgccgc
      301 gcacctgccg gagatcgtgt gcctgaaccg ggaggtggtc gcggtcgggc ctccccgacg
      361 ggtcctgacg ccggaggtcc tcgagcggac gtacggcgcg ccgatggagg tgctgcacca
      421 cggggggatg ccggtggtcg tcgatcatcc cgaggacgtc gaggtgctgc acggggagga
      481 gcaccaccat gagcatcctg cttgagccgt tcgcgtacgg gttcttcgtc agcggcgtcg
      541 tcgtcgcgac gctcgccggc gtgctctgcg gcctcgtggg ggtctacgtc gtgctccgcg
      601 ggatgagcta catcggccat ggcctctcgc acgcgatctt cgggggcgcg gtggcgagtt
      661 tcgtggcgaa ggtgaacttc tacgtcggcg cggcggcgtg ggggttcgtc gcggccctgc
      721 tcgtcaaccg cgtggcgcgg cggcgcacga tcggggccga cgccgcgatc ggcgtcgtca
      781 ccaccgcgag cttcgcggtg gggctcgcgc tcatctcgcg cttccggagc ttcacgcgga
      841 acttcgaggc ggcgctgttc ggcaacgtgc tcggcgtgcg cctccaggac gcgctggtcg
      901 tcggcggcgt cctgctcgtc gcggcggcgt tcgtcttttt cgcctaccgg ccgctgctgt
      961 tcaccacgtt cgaccccgag gtcgccgaag cgagcggcgt cccgacgggc cggatcgacg
     1021 ccggcttcgc gctcctgctc gccgcgacga tcgtcgccac gatgaacatc ctcggcgtcc
     1081 tgctgatcgc cgccgccctc gtgatccccg cggtcgtcgc ccgcctgctc accgactcgt
     1141 tcggccggat gctctggctg tcgatgctcg tcggcggcct ctccggcttc gcgggcatgt
     1201 acctgtcctg gtacctgaac gtcagctccg gcgccacgat cgtcctcacc caggccgcgt
     1261 tcttcatggt ggccttcgcc ctcacgggct tgcgccgccg ccacgggatc gcgccgaggc
     1321 cagccgttcc tccaggcacc cagtccggct ggtagccaag gctcgccggc cgggctccgc
     1381 ccggcccgag tgcttcctat gatgtcggcg ggatagtgat gagtggcgcc gatgaaactc
     1441 gcagagatcg aaccgcaaga gatctgcgtg cgtgcggttt cttggcgatc gcagcggccc
     1501 tctttgtagg cgctttcacc ctatgggcga gaggggttga gcccgagtcc ttcgcacaag
     1561 cacgttgcga gcgattcctg gaaggggaaa gcacagcaga gccagcggag tggtccgcat
     1621 gtatgcaaga acaacgagcg aaacccatcg tcacgttgct actgccgagc tggatcttcg
     1681 tcctactcgg gttgcttttc ctcatcctgg cgaatcggaa acagactcgt gagggccgcg
     1741 gctcgccgct gaccccacag cgacgccgac gaccgccgca ttcgaagccg cgcggagtag
     1801 accgcggtta gaagagaggc cggccgcggg gccatccgca agcgaggcga ctgccgtgat
     1861 ctcaacgcgt ttcggcgctg cagcgggcgc agaggccgga gatcgcgaag tgcgtgaggt
     1921 cggggtcgaa gccgcggtgg cggcggatca ggtcgcggag ggagccggcc tcggccatcg
     1981 aaaggtcgtc ctcggcgccg cagcgggagc aggtcaggtg gacgtggtcg ccctcgccgg
     2041 cgcggtggta ctcggccccg ctctcccggt gcgcgtgctg gacgacgccg agatcctcga
     2101 gcgtggcgag cgtgcgatag accgtcgagg cgttgaccgc gggcatccgg cgacggacgc
     2161 gccgggcgac ggcctggggc gtgatgtgcc cgtccgtccg gaggacctcc tcgatgatcg
     2221 cctcgcgctg cggcgtcagg cggtgaccgc gggcccgcag ccgaccgatg atctcctcga
     2281 cctcgcgcac gcttcgacgt tactgcaacg ctgctgcaac gcctagagcg gggtcagcgc
     2341 ggcggtcccg aggatctgga cgccccaggg atccgtcccc ggcgggttga gcgcgctttc
     2401 gaaggtcagc cggttccggg gcccgagacc tcgcagcggc acccgctgca ccccgcccca
     2461 ggcaccgggc tgggtggctc ggaggatccc gaccggcttc ccgttcagca cgacgcggat
     2521 ctccccaggc gagacgtcgt acgcgcggac gttcgcccac ccggcggcac cgccggaggg
     2581 gaaggcgaag gtcacgcgca cgccgccctc gagctgggtc gccgtcgggt cgacgctcca
     2641 ctgcccgggc ggcggcggga gcgcgacgac cgtcggcggc ggcgccgggg ccgcgggcgc
     2701 gatcgccggg tcggccgggc gaggcagccg cgccttcagc gcagcgagcg caccccacac
     2761 catgcggccg ctggtcgccg cgtcgtacat gctggcgccg atcgcgccgc cctcgcggac
     2821 ggcccggtcg aagccgcgca tgtcggcggc gctgacttcg ttcgcgatcc cgccgatcac
     2881 gtggatcggc acgtcgggcc ggcccgtgcc ctggcggatc agctggatgc tccgcgcgat
     2941 gaacgcgtgc gtgccacgct cgccggaggt gcggtaggtc cagtacgcca tcgggaggaa
     3001 ggcgtcgtag tgcggcgcga tccccgcgta cgggaagtcg ctccagtacc cggggtggat
     3061 ccgtccgccg tccttcgacg ggacgaggat cgaggggatg atcgccccca gcgggtacga
     3121 gcgcccgacg gcggcgcgga ggtcgtgcga cagccggacc agccggcggc tccgctcggc
     3181 gtggtcgcgg accttcgtga tctcgatgtc gagcgcgaag ccgtcgaagc ttcggccggt
     3241 cgcgctgcgg aagcggatcg ccgccagcga ccaccggagg tcacggcggg gccggtggaa
     3301 gtcggggacg taccagccga cgacgcgcat cccgttcgcg tgagccgcgt cgaggaagcg
     3361 cgaggtcccg ccggggaagc gcagcgggcc gccctccttg gggttcgtcg tctggaggta
     3421 gagcgtctcg acgccgcggg cgcggaggtc ggccacggtc gcttccgggg cggcccaggc
     3481 ggcgtcgtcg aagatgtcga cccaggcacc gaggccccgg aagggctcca ccggcgagcg
     3541 cgccggggct ggtgcggccg gaacccacag cagcagggcc gtcaggagga cggcgacgcg
     3601 gtgcatggtg atggcttcga cgcgcacgct cgcttccctg cgcctcacgc cggcgccagc
     3661 gtgacgcccc ggacgcccca ggtggtccag ggatgccgga tccgcggcac gaacgcgatc
     3721 acgttcttcg cgtcgggccg gagcgccgag gccgggatca ggatcgtctg ccgcgcgggg
     3781 gtccagtcct ccgggggtcc cgccgcgagg ggcaccacga gccgggtcca gttcacctgg
     3841 acctccacct ccccggcgcc gatgtcgaag ccctcgaagc tgagcacctg atccgagctg
     3901 agcgggccgt gccggtagac gacctcatcg gggtgcgtcc ggtcaccgcc ggtgatccgg
     3961 ccatagccgt cggtgtgctc gccgaaccgc agcggcatcg gcggcgactg ccgcggcggg
     4021 ttcggctgga cgcgggcgag ctcctcccag tcctccgccc cgaagagcga gtcgtcgtag
     4081 aggctgccac ccagaaggcc gtgctcacgg acgctccgga cgtacgcgcg ggtctcggat
     4141 cgggacgatc cgtcggccag tccaccgatc gcatggatcg gcacgcctgg gtcctcggtc
     4201 cggctgcgca ggatccggat cgagcgccgc atgtagtccg tcacctcagc ggcgcccgag
     4261 gctcgccacg agtagtaggt catcggcacg aagacgtcgt agcgttccgc gagcgcccgg
     4321 tacgggaagc gcggccagta cccgggggcg agctgcatcc cgcgtggcga cgggatgatc
     4381 gcccccatgg ggtagctcga accgacccgg gcccggagga agtccgacag tcgcaggagc
     4441 cgccggttcc gtgcgtcgat gtccttgacg atcgtggcct cgatgtcgag cgcgaacgag
     4501 tggaaccgcc gcccctcggg gctgcggaac cggatcgctg ccatcgcccg gcgttggtcg
     4561 cggtagaggt ccttcagccc ggggaggtac caggcgacga tcttcatccc gtgccggtgc
     4621 gcggcgtcga cgaaccgccc cgtcgccgca gggaaacgga tgtcccccgg ctggtggtag
     4681 ttgttcgact ggaggtagag ggtccggacg ccgcgggctg cgagccggcg gaccttccgg
     4741 cccggttggt cccatgcggt cgggtcgtag atgtcgatcc aggcgccgag gccgcggtag
     4801 gccgcgagcg agggggccgc ctgcgccggc gaggccggca ggagggacgc ggcgagggcg
     4861 gcgacgatga ggatggacct ggtgcgcagg gtgctccctc ccgggggtgg tgatgccatg
     4921 ataggccgcg ttcgcgccca agcagaagga ggggatatgg gggagctggt ccgcctggag
     4981 gtggaggagg gggtcggcac gatccgtctc gaccgcccac cgatgaacgc gatcaacgcc
     5041 gagctcgtta ccgacctccg ggccgcggcg ctcgaggcgg ccgagcgcga ggacgtgcga
     5101 gcggtcgtgc tctacggcgg cgagaaggtc ttcgccgcgg gcgccgacgt gaagatgatg
     5161 gcggagatgg ggcccgccga gatccgcccg gtgatccgga tgatgcagga gaccttcacc
     5221 ctcgtcgagg agatccccaa ggtgacgatc gcggccctca ccggctacgc gctcggtggt
     5281 ggctgcgagc tggcgctcgc cgcggatctc cggttcgccg ccgaggacgc gaggatcggg
     5341 caacccgaga tcctcctcgg gatcatcccc ggcgctggcg gcacccagcg gctgccgcgg
     5401 ctcgtcgggg tctcgcgggc gaaggacctg atctactccg ggcgccatct ccgcgcgcag
     5461 gaggccctcg agatcggact cgtcgacgcc gtccacccct cgggcgaggt gtacggggcc
     5521 gccgcggagc aggcccgccg gtacgccgcc ggcccgaccc aggcgctccg cgccgcgaag
     5581 atcgcgatcg accgcggcgt ccgcaccgac atcgggaccg gactcgcgat cgagcgggag
     5641 gttttctgcg acctgttctt caccgaggac cagcgggaag ggatgcggtc cttcgtcgag
     5701 caggggccgg ggaaggccac cttccgcggc cggtgaggat gaaacgggag gttccggact
     5761 tccgcgtgtg cgcctgtcca cgtgcgggta gaacccgacc actctggtcg ggaatacccg
     5821 ccacacccag ggcgtttccg atcgtgacga cccgaggggt gaggcatgag cgagtacatc
     5881 aaggggtccc ggccgcggca gctccccccg gccagccgaa cgtacggtgg cggccgtgtc
     5941 tgcgcggaac agggctgcga gacgcgcatc tcgatctaca acaaggcgac gcattgctgg
     6001 gcgcacgctc cgctgcggta cccggtcagc cggggcgagc gccgcaagaa gaccgcggcc
     6061 tgacgacctc tcgcggctgc ccggcgagcg cgtcgtagac tgcgcgaccg tatgccgccg
     6121 tccgggatcg cccccgaacg ccgacccccg cgcgccgcga tcggcctcct cctcgccggc
     6181 ctcgccgtct tcggactgct cgcggtgctc gtcctcctcg cggagcccgc cctgctcgcg
     6241 atcgatcgtc ctctgagcga ggccctgcgg gcggaccggg gcgacgtgct ggccgaggtg
     6301 atgcgcggga tcagcctcct cggcagccgc gcggtgatcg gcggcatcct cttggcgctc
     6361 acgctctggg cgatcgtgac gcgcgagtgc cagcgcacgg tgggcatcgt cctcgtggtc
     6421 ttcgcggtcg cgatggtcct cgagtggaca ctgaaggcgg ggatcggccg cctgcggccg
     6481 gaggaggcgt tcgcgctcac gcgcgtcacg acggccgcct tcccgagcgg ccacgccctc
     6541 ggatcggccg ccgtctacgc gatcctggcc gtcgcgctcg gcggcggcgc acgcgggcac
     6601 gcgctcgtgt ggctcctgat cgcggcgatc ggcgtgtcac gggtcgtgct cggcgtccac
     6661 tggccgagcg atgtcctcgg ggggatcgcc ctgggcgctg cgatcgccgc ggggggcgtc
     6721 ctcgcgcttc gcgggcatcg cctcgatccg aagcgctgcc ccggcgggca cgggacctag
     6781 acgggtccgc agccctccgc gcccaggaag acccgcaggg ccacgaacac ggtctcgtcg
     6841 aggagggcct cgacgcaggc atccgccccg cgctcgagtc cctcctcggg tgggatctcc
     6901 gtccacccgc cccccgcgcg acggaacacg gccgtcgcgt cgccggcgag acgctccaag
     6961 cgcacgcgcg gtccctcgcc gctgcgg
//
LOCUS       DATLGG010000232         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_93699, whole genome shotgun sequence.
ACCESSION   DATLGG010000232 DATLGG010000000
VERSION     DATLGG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93699"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VM638_06635"
     CDS             <1..662
                     /locus_tag="VM638_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12133.1"
                     /translation="AGAWGRRVAAAVTDMNQPLGTAIGNALDVAEAVAILRGERGGRL
                     LDLVIELAAAAQVALGGAEEGEARRHARDAIASGRAAETFRAMVAAQGGDPRVIDDPA
                     AVLPSAPVVRPILLERSGVLGGVDAEGLGRAAAALGAGRRRKGDPVDPAVGIVFAPKI
                     GDEIHAGGTAGEIHARSEDAADEAHRRVVSALEVVDGPVDPPPLVYGWRSEGSDPSRT
                     S"
     gene            623..1285
                     /locus_tag="VM638_06640"
     CDS             623..1285
                     /locus_tag="VM638_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVM12134.1"
                     /translation="MALGRERPIENVLTVVFFVVALVPAIVLHEVSHGYVADRFGDHT
                     ARMAGRLSLNPIRHVDPFGTVILPGLLLLPHLFGRGQNPVFGYAKPVPINPANLSHPD
                     RQTMWIALAGPATNLGLAIVGALGFRFVGAGAGEISRFLFIFVAVNVLLAVFNILPIP
                     PLDGSKVLARYLPPRAREIYRSWEAYGALFMLVIFFLFPGAIFGIVDPIVTGLFGLLV
                     GS"
     gene            1326..2330
                     /gene="trpS"
                     /locus_tag="VM638_06645"
     CDS             1326..2330
                     /gene="trpS"
                     /locus_tag="VM638_06645"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVM12135.1"
                     /translation="MEPASPQAPDRRARVLTGYRPTGPLHVGHWFGNIQNMLELQREH
                     DAFFFVADWHALTTDYDRTERLPENVRNLVLDWLAAGIDPAAATIYLQSDVPEVAELT
                     LLLGMMTPLGWLERVPTYKERLRDLAEREIANYGLLGYPLLQTVDIAIVRGELVPVGE
                     DQVAHLELAREIVRRFNGLYGDILVEPQALLSETPRIPGTDGRKMSSSLGNDIRINDA
                     PDVMMEKARSMVTDPQKVRLGDPGRPEVCPVFQLHKRFSTDILEETRAGCSTGTLGCV
                     KCKTNLGERMVASFAPFQERRRDLDPASAQDALTRGREKARPVAERTMKDVREAMNLT
                     "
     gene            2327..3073
                     /locus_tag="VM638_06650"
     CDS             2327..3073
                     /locus_tag="VM638_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HVM12136.1"
                     /translation="MSEPVGTQVGGFPVELPVFTGPFRVLAELLLNGKVDVCDVRVAT
                     VTDRFLARAQEAEGWTLEEATWFLAVGAALLEMKVGRLMPKHREPDEEDLIGGSPDLA
                     YARSLELRAFRRVAGWVAERLETWSKLHPREVGPAEEFADLYPDLMEKVTASGLAEAA
                     AALLRSPTVDLTHVQPIRASVTEALEALSERLTAGRREPARFRELVADCREPIQVVVR
                     FLALLELYKDGRVALSQAETFGEIEVRWEG"
     gene            3073..3639
                     /gene="scpB"
                     /locus_tag="VM638_06655"
     CDS             3073..3639
                     /gene="scpB"
                     /locus_tag="VM638_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVM12137.1"
                     /translation="MQRDPLTRDLEALLLVAAEPLPAAALASVVEVPRREVEEALQRL
                     SDRYEESGSGILLRNVAGGWRLFTHPDANEVVERFILSSRHARLSRAALETLAIVAYK
                     QPVTRHQVAAIRGVNSDGVLKALTDRGLLQEVGRDEGPGRPLLYGTTPEFLERLGLAS
                     VQDLPPLAPLLGEEEPEEDGAGEAAAGA"
     gene            3611..4384
                     /locus_tag="VM638_06660"
     CDS             3611..4384
                     /locus_tag="VM638_06660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVM12138.1"
                     /translation="MPEKRPPAPEPERLQKVLARAGLGSRRACEELVRDGRVTVNGEI
                     ADLGRRVDTARDHVEVDGLRVPVNPDLVYFALNKPSGVVTTARDPQRRPDVSRYYPRG
                     VRVFPVGRLDRDTEGLLLLTNDGELANRLTHPRYGAEKEYLAEVEGAVADRLLRRLER
                     GVDLEDGLARAVAARRAAAAGGRTAVRMVMTEGRKREVRRMLAAVGLRVTRLVRVRVG
                     PLRLKDLPPGSVRPLTPLEVRDLYGHVEEAERRARRRKP"
     gene            4381..>4761
                     /locus_tag="VM638_06665"
     CDS             4381..>4761
                     /locus_tag="VM638_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVM12139.1"
                     /translation="MIPKAGLVVAIDGPAGAGKSTLASRLAGALALPCVTTGVMYRAV
                     AREALDRGVDVEDEVALAEIAAGIRFSLGDDDPPGLLVDGRPPGDELVGTDVEAVVSA
                     VARHPRVRSILRERQRAIASSGGVV"
BASE COUNT          628 a         1687 c         1756 g          690 t
ORIGIN      
        1 tcgcgggggc gtgggggcgg cgcgtcgccg cggccgtcac ggacatgaac cagccgctcg
       61 gaaccgccat cgggaacgcc ctcgacgtcg ccgaggcggt cgcgatcctg cgtggcgagc
      121 ggggcggccg gctcctcgat ctcgtgatcg agctggccgc ggcggcccag gtcgcgctcg
      181 gcggagcgga ggaaggcgag gcgcgccggc atgcgcggga cgcgatcgca tccggccgcg
      241 ccgccgagac cttccgggcc atggtcgccg cgcagggcgg ggacccccgg gtgatcgacg
      301 atcccgccgc ggtcctgccg agcgccccgg tggtccggcc gatcctgctc gagcggagcg
      361 gcgtcctcgg cggcgtcgac gccgaaggac tcggccgagc cgccgcggcc ctcggcgcgg
      421 gccgacgacg gaagggcgac cccgtcgatc cggccgtcgg catcgtgttc gctccgaaga
      481 tcggcgacga gatccacgct ggtgggacgg ccggcgagat ccacgctcga tcggaggatg
      541 cggccgacga ggcccatcgt cgcgtcgtgt ccgcgctgga ggtcgtcgat ggcccggtcg
      601 acccgccccc gctcgtgtac ggttggcgct cggaagggag cgacccatcg agaacgtcct
      661 gaccgtcgtc ttcttcgtgg tcgccctcgt gccggcgatc gtcctgcacg aggtgagcca
      721 tggctacgtc gcggaccgct tcggcgacca caccgcgcgg atggccggcc ggctgagcct
      781 caacccgatc cgccacgtcg acccgttcgg gaccgtgatc cttccgggcc tgctgctgct
      841 gccgcacctg ttcggtcgcg ggcagaaccc ggtgttcggc tatgcgaagc ccgtcccgat
      901 caaccccgcg aacctgtcgc accccgaccg ccagacgatg tggatcgcgc tcgccggtcc
      961 ggccacgaac ctcggcctcg cgatcgtggg ggcgctcggc ttccgcttcg tcggcgccgg
     1021 ggcgggcgag atctcgcggt tcctgttcat cttcgtcgcg gtgaacgtcc tgctggcggt
     1081 cttcaacatc ctgccgatcc cgccgctcga cgggtcgaag gtcctcgcgc gctacctccc
     1141 gcctcgcgca cgggagatct accgctcctg ggaggcgtac ggggcgctct tcatgctggt
     1201 gatcttcttc ctgttcccgg gggcgatctt cgggatcgtc gacccgatcg tcacgggact
     1261 gttcgggctc ctcgtcgggt cgtgagtgat tgaggcgctc cggcgccggg gctagcatgg
     1321 accccgtgga acccgcctcc ccccaggctc ccgaccggcg cgcgcgcgtg ctcaccggct
     1381 accgtccgac cgggccgctc cacgtcggcc actggttcgg gaacatccag aacatgctcg
     1441 agctccagcg ggagcatgac gccttcttct tcgtggccga ctggcatgcc ctcacgaccg
     1501 attacgaccg caccgagcgc ctcccggaga acgtccgcaa cctcgtcctg gactggctcg
     1561 ccgccgggat cgaccccgcg gcggcgacga tctacctgca gtctgacgtg cccgaggtgg
     1621 cggagctgac gctgctcctg gggatgatga cgccgctcgg gtggctcgag cgcgtgccga
     1681 cctacaagga gcgactgcgt gacctcgcgg agcgggagat cgcgaactac gggctcctcg
     1741 ggtacccgct cctgcagacc gtcgacatcg cgatcgtccg cggggagctc gtgcccgtcg
     1801 gcgaggacca ggtggcgcac ctcgagctcg cgcgcgagat cgtgcgtcgc ttcaacgggc
     1861 tctacggcga catcctcgtc gagccgcagg ccctcctgtc cgagaccccg cggatcccgg
     1921 ggaccgacgg gcggaagatg tcctcctcgc tcgggaacga catccggatc aacgacgcgc
     1981 cggacgtgat gatggagaag gcgcggtcga tggtgacgga cccgcagaag gtccgtctcg
     2041 gagatccggg gcgcccggag gtctgtccgg tgttccagct ccacaagcgc ttctcgaccg
     2101 acatcctcga ggagacccgg gcgggctgca gcaccgggac cctcggatgc gtgaagtgca
     2161 agacgaacct cggcgagcgg atggtggcct cgttcgcacc gttccaggag cggcgtcgcg
     2221 acctggatcc cgcctcggcc caggacgccc tgacgcgagg ccgcgagaag gcccgtccgg
     2281 tggccgagcg gacgatgaag gacgtgcggg aggcgatgaa cctcacgtga gcgagccagt
     2341 cgggacgcag gtcggggggt tcccggtgga gctgccggtg ttcaccggtc ccttccgggt
     2401 cctggcggag ctcctcctca acgggaaggt ggatgtctgc gacgttcgcg tcgcgaccgt
     2461 caccgaccgc ttcctggctc gtgcgcagga ggccgagggc tggaccctcg aggaggcgac
     2521 gtggttcctc gccgtcggag cggcgctcct cgagatgaag gtcggacgcc tgatgccgaa
     2581 gcaccgggag ccggacgagg aggacctcat cggcggctcg ccggacctcg cgtacgcgcg
     2641 gagcctggag cttcgggcgt tccggcgggt ggcggggtgg gtagcggagc ggctcgagac
     2701 ctggtcgaag ctgcacccac gcgaggtcgg cccagccgag gagttcgccg atctctatcc
     2761 cgacctcatg gagaaggtga cggcgagcgg tctcgcggag gcggcggccg ccctcctgcg
     2821 ctcgcccacc gtggacctca cgcacgtcca gccgatccgc gcatccgtga cggaagcgct
     2881 cgaggcgctg tccgagcggc tgacggccgg gcggcgggag ccggcgaggt tccgcgagct
     2941 cgtcgcggac tgccgcgagc cgatccaggt cgtggtccgc ttcctcgccc tgctcgagct
     3001 ctacaaggac ggccgggtcg cactcagcca ggccgagacg ttcggcgaga tcgaggttcg
     3061 gtgggagggc tgatgcaacg tgatccgttg acgcgggacc tcgaagccct gctgctcgtc
     3121 gcggccgagc ccctgcccgc ggcggcgctc gcgtcggtgg tcgaggtgcc gcgccgcgag
     3181 gtcgaggagg ccctgcaacg tctctccgac cggtacgagg agagcggctc cggcatcctg
     3241 ctccgcaacg tggccggcgg ctggcgcctg ttcacccacc cggacgcgaa cgaggtcgtc
     3301 gagcgcttca tcctgtcgtc gcgccacgcg cgcctcagtc gcgccgctct cgagacgctc
     3361 gcgatcgtcg cgtacaagca gccggtcacg cgccaccagg tggcggcgat ccgcggggtg
     3421 aactccgacg gcgtcctcaa ggcgctcacc gaccgtggtc tcctccagga ggtcgggcgc
     3481 gacgagggac ccgggcgacc gctgctgtac gggacgaccc cggagttcct cgagcgcctc
     3541 ggactcgcct ccgtccagga tctgcctccc ctcgcgcccc tcctcggcga ggaggagcct
     3601 gaggaggacg gtgccggaga agcggccgcc ggcgcctgag cccgagcgcc tccagaaggt
     3661 gctcgcgcgc gccggcctcg gatcccgccg cgcctgcgag gagctggttc gcgacggacg
     3721 cgtcaccgtg aacggcgaga tcgcggacct cggccgccgg gtcgataccg cgcgcgacca
     3781 cgtcgaggtg gacggcctgc gcgttccggt gaacccggac ctcgtgtact tcgcgctcaa
     3841 caagccctcg ggcgtggtga ccacggcgcg cgatccgcag cggcgccccg acgtctcccg
     3901 ctactacccg cggggtgtgc gggtgttccc ggtcggccgg ctcgatcggg acacggaggg
     3961 actgctgctc ctgacgaacg acggcgagct ggcgaaccgt ttgacccatc ctcgctacgg
     4021 tgccgagaag gagtacctcg ccgaggtcga gggcgcggtc gcggaccggc tcctgcgtcg
     4081 cctcgagcgc ggggtcgacc tcgaagacgg cctcgcgcga gcggttgcgg cgcgtcgcgc
     4141 cgccgcggcg ggcgggcgga cggcggtgcg catggtcatg accgagggac gcaagcggga
     4201 ggtccgacgg atgctcgcgg cggtcgggct gcgggtcacg cggctcgtgc gtgttcgcgt
     4261 cggacccctt cgcctgaagg acctgccgcc gggatcggtc cggccgctca cgccgctgga
     4321 ggtccgcgac ctgtacggtc acgtcgagga ggccgagcga cgcgcgcgca ggaggaagcc
     4381 gtgatcccga aagcggggct ggtcgtggcg atcgacgggc cggccggcgc gggcaagagc
     4441 acgctcgcga gccgcctcgc cggagccctc gccctgccct gcgtcacgac cggcgtgatg
     4501 taccgggccg tggcgcggga ggcgctcgat cgcggcgtcg atgtcgagga cgaggtcgcg
     4561 ctcgccgaga tcgcggcggg catccgcttc tccctcggcg acgacgaccc tcccgggctc
     4621 ctggtcgacg ggcgaccgcc gggcgacgag ctcgtgggca ccgacgtgga ggccgtggtg
     4681 tcggccgtcg ctcggcaccc gcgggtccgg tcgatcctgc gggagcggca gcgcgcgatc
     4741 gcctcgtcag gcggtgtcgt c
//
LOCUS       DATLGG010000233        11254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_151824, whole genome shotgun sequence.
ACCESSION   DATLGG010000233 DATLGG010000000
VERSION     DATLGG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151824"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /gene="dnaK"
                     /locus_tag="VM638_06670"
     CDS             complement(<1..1753)
                     /gene="dnaK"
                     /locus_tag="VM638_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HVM12140.1"
                     /translation="MPKAVGIDLGTTNSVVAVLEGGEPTVIPNAEGARTTPSVVGFSK
                     TSEILVGEVAKRQAITNPDRTVRSVKRHMGERDWSLEVDSKKWSPQEISAQILQKLKR
                     DAEAYLGDTVNQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAAALAYGLDK
                     TETDQTILVFDLGGGTFDVSLLEIGDGVFEVKATAGNTQLGGDDWDQRVMDWMVKEFK
                     NAHGIDLSKDRMAMQRLREAAEKSKIELSQVSESHINLPFITAGADGPLHLEMKLTRS
                     EFERMTEDLVQGCEGPFNQAIKDWGKAASDIDHVILVGGSTRMPMIQQLVKELTGGKE
                     PHKGVNPDEVVAVGAAIQAGVLRGEVKDVLLLDVTPLSLGIETKGGIFTKLIERNTTI
                     PTKKSETFTTADDNQTQVEVHVLQGEAETVYSPGVRSLGRFQLMGIAPAPRGMPQIEV
                     TFDIDANGIVNVSAKDTATGKEQAMTITGGTALGKDEIDRMMKDAESFAEEDRRRREA
                     AEARNNADNLAYQAEKQLQELGDKVPADDRTALEQAIGEVRKALEGDDVDEIRAKTDA
                     LMQAFQKVGQAVYQQQQG"
     gene            complement(1911..2714)
                     /locus_tag="VM638_06675"
     CDS             complement(1911..2714)
                     /locus_tag="VM638_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12141.1"
                     /translation="MTKQTGSAIRSFVAALLAAVVLAAAGVSAAKQVGMQLASGEAEL
                     IQDGGTGDGSGEGSDELTCPEDGSTDDGTSDDGTTDDGTSDDGATDDGASDDGSSEEE
                     VFASALDGDDSGTGDGSETGGEEGCPSDDADADPKPEMSDDADPTVAREEACAAAAGV
                     SPAGEEGDEAPGPAKGLDRALQVVGENCVKNPQAPGLLVALERLSANKAKQEERKAAR
                     QARREARAAERAAKRAAKAGGGEIGVPSALGGGQGGHGGKGKGKGRGKG"
     gene            2812..4836
                     /locus_tag="VM638_06680"
     CDS             2812..4836
                     /locus_tag="VM638_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVM12142.1"
                     /translation="MLRILILLFFLGTAGGLFMLRAWTLFRLVRLGQPVKRTDDLPKR
                     VQKEVVVSLGQKKLLQRLGPGLMHAFIFWGFLVLLTTVIEVFGEVVDERFAIPFIGRS
                     GWLGLVQDAFAGLVLVGIGMAIYFRKVRRDERFKGSHLEEADFILLMIFGIIFTLLFL
                     NAVKIAQGMNESPAAWTPLSNAIAPLFRPMGEAALGFFRGLFLWSHILLILGFLVYIP
                     YSKHLHIVTSFFNVFFSKTKPMGKLRTLDIDVENLEGSSLGAATVEDLTWKQVLDTFT
                     CTECGRCQDVCPAWNTGKVLSPKLLIMNLRDHILDEGQEILWARAEGEEREKVPLNPN
                     VVEDQQVWDCTTCGACMRECPVNIEHVDHIIDMRRNLVMAESRFPQEAGLLLRNLESA
                     KNPWGMPQATRADWAEGLGVRVLEDGEAPEYLYWVGCAAAFDDRSKKIAQATARVMQK
                     AGLRFAILGPRERCNGDPARRLGNEFLFQELARENVETMNGIGVRKIVVNCPHCFNTI
                     RNEYPDFGGDYEVIHHTQLFARLVEEGKLRPTEEVRSLITYHDPCYLGRHNGVYEEPR
                     ELLEAIPGVRQVEMPRHAERGFCCGAGGARMWMEERIGKRINEERTDEAAATGADVMG
                     VACPFCLVMLDDGAKARGADIQVIDIAQVVAESVGLGDRAPAIEGAESTS"
     gene            4894..4964
                     /locus_tag="VM638_06685"
     tRNA            4894..4964
                     /locus_tag="VM638_06685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4926..4928,aa:Gly,seq:ccc)
     gene            complement(4967..5587)
                     /locus_tag="VM638_06690"
     CDS             complement(4967..5587)
                     /locus_tag="VM638_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12143.1"
                     /translation="MGERGPLFFTSMLSLTVLSGLLTLILLVQQTVPPPSALAPEHLD
                     PPVGGPPVRDGAGGPPEAPFPTLGLDLTTSGGVIGIGITPSVGGPQGGGGAGPDDGRP
                     RRPRPAPEPEPGVTPRTDPPPDDDDEDGPGKGPKSKGGNKGKKVGHHKGHGKGHGGSH
                     GHGHLHHEASASTASSIGGGPSAKAKTNAGANAKGRSKAKGRGARG"
     gene            complement(5813..7168)
                     /gene="dnaB"
                     /locus_tag="VM638_06695"
     CDS             complement(5813..7168)
                     /gene="dnaB"
                     /locus_tag="VM638_06695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HVM12144.1"
                     /translation="MKAADDVRALRTERVPPHNLEAEASVLGSMMVSDEAIAGVTEVV
                     KAEDFYRSAHRTIYSAMLRLYAQGEPVDAITTVEELRRLGHLDTIGGPLYIHHLVESV
                     ATPASASHYARIVAEHALLRRLIDAAWTIVDRAYKVPEDPVGLADEAEGLVYGVARHR
                     DKEQAVPLSDLVSETMEALERLHERDSAFAGVPTGFQDLDSLLQGLHPGNLIVVAARP
                     GVGKSSFVTNIARNVVVETGKPVAMFSLEMSRSEIAMRLLCAEARVPWDKVRAGRIGA
                     EDWARIAEAAERLDQAPLWIVDSGNVNIVDIRAKARRLRSSREGLGLIIVDYLQLMSS
                     HIRVENRQQEIAEISRSLKMLAKELEIPVIAVSQLNRDPEKRQDKRPQLSDLRESGAI
                     EQDADVVMFVHREDAFNDDPAVKGLAEIIVAKHRNGPQDRIKLTFLSHLTQFRNFARE
                     P"
     gene            complement(7610..8061)
                     /gene="rplI"
                     /locus_tag="VM638_06700"
                     /pseudo
     CDS             complement(7610..8061)
                     /gene="rplI"
                     /locus_tag="VM638_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
     gene            complement(8058..8219)
                     /gene="rpsR"
                     /locus_tag="VM638_06705"
     CDS             complement(8058..8219)
                     /gene="rpsR"
                     /locus_tag="VM638_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVM12145.1"
                     /translation="MDYKDTATLRRSISDRGKIRARRVTGTCSQHQREVAAAVKTARQ
                     LALLPYSTR"
     gene            complement(8370..8816)
                     /gene="ssb"
                     /locus_tag="VM638_06710"
     CDS             complement(8370..8816)
                     /gene="ssb"
                     /locus_tag="VM638_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVM12146.1"
                     /translation="MANGNQVIVMGNLTDDPELRYTPNGAAVANFRVAVNRRYQDQSG
                     QWREEETSFFTVTAWRSLGENAAESLTKGSRVIVAGRLRSRSYETQEGQKRTVVEIEA
                     EDLGPSLRWATAKVEKQTGSGGGGGDWNANVAVGAGSSEEAPFEGA"
     gene            complement(8885..9172)
                     /gene="rpsF"
                     /locus_tag="VM638_06715"
     CDS             complement(8885..9172)
                     /gene="rpsF"
                     /locus_tag="VM638_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVM12147.1"
                     /translation="MRDYEVMVIFDPDADQAAIDGVVSRATSLLAERGGEVESSETWG
                     KRKLAYEIDHRSEGIYVLVKFRAEPGDLTELERVLTLADQVLRHKVVKRAA"
     gene            complement(9265..10329)
                     /locus_tag="VM638_06720"
     CDS             complement(9265..10329)
                     /locus_tag="VM638_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12148.1"
                     /translation="MRRRVARILSGALLFCTVLAFVPPIRARATGVGVVAEAVGLGLP
                     RPFAHDVARVEVTVAGVVGDAYLPAGRAPLVVLVPGAAMDARRDERLVRLARALGRAG
                     RIVFIPELALARERLDERDIDRIVAVTAALDAHPRTEGRPVMFGISFGGSFSLVAAAD
                     PRIRDRLAQVAVFGAYWELGGLLQAVTTRTSVVGDRRIPFEPHPLAGEVLREVALALV
                     DPAERPALRAVLRGQRDPATLPAGAAAIHALLRNRVPERTPRLIELLPGRARRLLERL
                     SPAAAAPGLRAPVVAMHSTDDAAVPHAELLRLQHALPEARTLTVSLFRHVEFEGGDVP
                     GALRDLWRGATFVAWLLEAP"
     gene            complement(10334..11122)
                     /gene="panB"
                     /locus_tag="VM638_06725"
     CDS             complement(10334..11122)
                     /gene="panB"
                     /locus_tag="VM638_06725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVM12149.1"
                     /translation="MTTIHDLAAWKAQGRRFAMLTAYDHPTARILDRAGIPVILVGDS
                     LANVVLGYETTLPVTMEEMLHHAKAVARGAEHALLVGDMPFMSYQASVEEGVRNAGRF
                     LKEGGMHAVKLEGPLFQLAEALTSRGIPVMGHLGLTPQSVHSMGGYRVQGRSEEDADR
                     LLSDALTLEKAGIFSLVLEGVPSDVARRVTTSLRVPTIGIGAGPHTDGQVLVVNDLLG
                     LGEGEPPRFVKRYADLRRDIERAVHAFADEVERGVFPDEGHSYA"
BASE COUNT         1544 a         4045 c         3850 g         1815 t
ORIGIN      
        1 gtccctgctg ctgctggtag accgcctggc cgaccttctg gaacgcctgc atcagcgcgt
       61 cggtcttggc gcggatctcg tccacgtcgt cgccttcgag cgccttgcgg acctccccga
      121 tcgcctgctc gagcgcggtg cggtcgtcgg ccgggacctt gtcgcccagc tcctgcagct
      181 gcttctcggc ctggtacgcg aggttgtcgg cgttgttgcg cgcctccgcg gcctcgcgac
      241 ggcggcgatc ctcctcggcg aacgactcgg cgtccttcat catccggtcg atctcgtcct
      301 tgccgagggc ggtgccgccg gtgatcgtca tcgcctgctc cttgccggtg gcggtgtcct
      361 tcgccgagac gttcacgatc ccgttcgcgt cgatgtcgaa ggtcacctcg atctgcggca
      421 tcccacgcgg cgccggcgcg atccccatca gctggaaccg cccgaggctc cgcacgcccg
      481 gcgagtagac ggtctcggcc tcgccctgga ggacgtggac ctcgacctgc gtctggttgt
      541 cgtccgcggt ggtgaaggtc tcggacttct tcgtcgggat cgtcgtgttg cgctcgatga
      601 gcttcgtgaa gatcccgccc ttggtctcga tcccgagcga caggggcgtc acgtcgagga
      661 gcaggacgtc cttgacctcg ccgcggagca caccggcctg gatcgcggcg ccgaccgcga
      721 cgacctcgtc ggggttcacg cccttgtggg gctccttgcc gccggtcagc tccttcacca
      781 gctgctggat catcggcatg cgggtcgagc cgcccacgag gatcacgtgg tcgatgtcgc
      841 tcgcggcctt gccccagtcc ttgatcgcct ggttgaaggg gccctcgcag ccctgcacca
      901 ggtcctcggt catccgctcg aactccgagc gcgtcagctt catctcgagg tgcagtggcc
      961 cgtcggcgcc cgccgtgatg aacggcaggt tgatgtggct ctccgagacc tgggagagct
     1021 cgatcttcga cttctccgca gcctcgcgca ggcgctgcat cgccatgcgg tccttcgaga
     1081 ggtcgatccc gtgcgcgttc ttgaactcct tgaccatcca gtccatgacg cgctggtccc
     1141 agtcgtcgcc gccgagctgg gtgttgccgg cggtcgcctt cacctcgaag acaccgtcgc
     1201 cgatctcgag cagggagacg tcgaaggtcc ctccgccgag gtcgaagacg aggatcgtct
     1261 ggtcggtctc ggtcttgtcg agcccgtagg cgagggcggc cgcggtcggc tcgttgatga
     1321 tgcgcaggac ctcgagcccg gcgatctggc cggcctcctt ggtcgcctgc cgctgggcgt
     1381 cgtcgaagta cgccgggacc gtgatgacgg cctggttcac ggtgtcgccc aggtaggcct
     1441 ccgcgtccct cttgagcttc tggaggatct gagcggagat ctcctggggg ctccacttct
     1501 tcgagtcgac ctcgagggac cagtcccgct cccccatgtg gcgcttcacc gagcggaccg
     1561 tgcggtccgg gttggtgatg gcctggcgct tcgccacctc gccgacgagg atctccgatg
     1621 tcttggagaa ccccacgacc gagggcgtgg tccgcgcccc ctccgcgttg gggatgacgg
     1681 tcggctcgcc gccctcgagg acggcgacga cggagttcgt cgtccccagg tcgatcccaa
     1741 cggccttcgg catgctgtcg ctccttcctc aggattcctt atacgccagc atgataggaa
     1801 gttgagcgtg tcgttgtcaa ctccgagggc gtgacccccg ggggtcggtg agggccagaa
     1861 cgcgagaagg gcgggcaagc ccgcccttct ccatgggatc gatcttggat tcagcccttg
     1921 ccgcgtccct tgcccttgcc cttgccgccg tggccgccct ggccgccgcc gagggccgag
     1981 ggaacgccga tctcgccgcc tccggccttc gcggcgcgct tcgccgcacg ctccgcggcg
     2041 cgagcctctc gccgggcctg ccgcgccgcc ttccgctcct cctgcttcgc cttgttcgct
     2101 gaaagtcgct cgagtgccac gagcaggccg ggcgcctgcg ggttcttgac gcagttctcg
     2161 ccgaccacct ggagcgcccg gtcgagaccc ttcgcgggtc cgggggcctc gtcgccctcc
     2221 tcacctgccg gcgagacgcc agccgcggcc gcgcaggcct cctcacgggc aacggttgga
     2281 tccgcgtcat cggacatctc cggcttcggg tcggcgtcgg cgtcatccga gggacacccc
     2341 tcctcgccac ccgtctcgct gccgtccccg gtcccgctgt cgtcaccatc gagcgcggac
     2401 gcgaagacct cctcctcgct cgacccgtcg tcgctggcgc catcgtcggt ggcgccatcg
     2461 tcgctcgtgc cgtcgtcggt ggtgccgtcg tcgctcgtgc cgtcgtcggt ggagccgtcc
     2521 tccggacagg tcagctcatc ggatccctcg ccggagccgt ctccggtgcc gccgtcctgg
     2581 atgagctcag cctctcccga tgccagctgc atcccgacct gtttggccgc cgagacacct
     2641 gcggccgcca gaaccaccgc ggcgagcagc gcggccacga acgatcggat ggcggaacct
     2701 gtctgcttcg tcatgcggag cagtatcggc tcggccggac tagtccttta ggcctaggcc
     2761 cgccgggccc gctggtttgc cgacctcgcc gtcactgcct agactcggcg cgtgctcagg
     2821 atcctcatcc tgctcttctt cctcgggacc gccggcggcc tgttcatgct ccgcgcctgg
     2881 accctgttcc gcctcgtccg gctcgggcag ccggtgaagc ggaccgacga cctcccgaag
     2941 cgcgtccaga aggaggtcgt ggtctccctc ggccagaaga agctgctcca gcgcctcggc
     3001 ccggggctga tgcacgcgtt catcttctgg ggattcctcg tcctgctcac gacggtgatc
     3061 gaggtcttcg gagaggtcgt cgacgagcgc ttcgcgatcc cgttcatcgg acgcagcgga
     3121 tggctggggc tggtccagga cgcgttcgcg ggcctggtcc tcgtcgggat cgggatggcg
     3181 atctacttcc gcaaggtgcg acgcgacgag cgcttcaagg ggagccacct cgaggaggcc
     3241 gacttcatcc tgctgatgat cttcgggatc atcttcaccc tgctgttcct gaacgcggtc
     3301 aagatcgccc aggggatgaa cgagtctccg gcggcctgga cgccgctttc gaacgcgatc
     3361 gccccgctgt tccggccgat gggcgaagcc gccctcgggt tcttccgagg gctcttcctg
     3421 tggtcgcaca tcctgctgat cctcgggttc ctcgtctaca tcccctactc gaagcacctg
     3481 cacatcgtca cgagcttctt caacgtcttc ttctcgaaga cgaagccgat gggcaagctg
     3541 cggaccctcg acatcgacgt cgagaacctc gagggatcga gcctgggcgc ggcgacggtc
     3601 gaagacctca cgtggaagca ggtgctggac accttcacct gcaccgaatg cgggcggtgc
     3661 caggacgtct gcccggcgtg gaacacgggc aaggtcctct ccccgaagct cctgatcatg
     3721 aacctgcgcg accacatcct cgacgaggga caggagatcc tgtgggcacg ggcggagggc
     3781 gaggaacggg agaaggtgcc cctcaacccg aacgtcgtcg aggaccagca ggtctgggac
     3841 tgcacgacct gcggcgcgtg catgcgcgag tgcccggtga acatcgagca cgtcgaccac
     3901 atcatcgaca tgcgccggaa cctcgtgatg gcggagtccc gtttcccgca ggaggcgggg
     3961 ctgctcctgc ggaacctcga aagcgcgaag aacccctggg gcatgccgca ggcgacgcgg
     4021 gccgactggg ccgaggggct cggggtgcgg gtcctcgagg atggggaggc tcccgagtac
     4081 ctctactggg tgggctgcgc cgccgccttc gacgaccgct cgaagaagat cgcgcaggcg
     4141 accgcgcggg tgatgcagaa ggcgggactg cggttcgcga tcctcggccc acgcgagcgc
     4201 tgcaacggcg accccgcacg tcggctcggc aacgagttcc tcttccagga gctcgcccgc
     4261 gagaacgtcg agacgatgaa cgggatcggc gtgcggaaga tcgtcgtcaa ctgcccgcac
     4321 tgcttcaaca cgatccgcaa cgagtacccg gacttcggcg gcgactacga ggtgatccac
     4381 cacacccagc tgttcgcccg gctggtcgag gaagggaagc tccggcccac cgaggaggtg
     4441 cggtccctca tcacctacca cgacccctgc tacctcggac ggcacaacgg cgtctacgag
     4501 gagccgcggg agctcctcga ggccatcccg ggcgtgcgac aggtggagat gccacggcat
     4561 gcggagcggg gcttctgctg cggcgccggg ggggcgcgga tgtggatgga ggagcggatc
     4621 ggcaagcgga tcaacgagga gcgaaccgac gaggcggccg cgaccggcgc cgacgtgatg
     4681 ggcgtcgcct gcccgttctg cctcgtgatg ctcgacgacg gtgccaaggc gcgcggcgcc
     4741 gacatccagg tgatcgacat cgcccaggtc gtcgcggagt cggtcggtct cggcgaccga
     4801 gcgcccgcga tcgaaggcgc tgagtccacc tcctagagca ggatcggcgg gccgggcgct
     4861 ccgccgcgtt cgctatactc ggaccccggt tccgcgggtg tagttcaatg gtagaacgcg
     4921 agcttcccaa gctcgaggcg ggggttcgat tcccctcacc cgctcgctag cccctcgccc
     4981 cgcgtccctt cgccttcgag cggcccttgg cgttggcacc ggcgttcgtc ttcgccttcg
     5041 ccgaggggcc acccccgatg gaggaggccg tggatgccga ggcctcatgg tggaggtgac
     5101 cgtggccgtg actgccgccg tgccccttcc cgtggccctt gtgatggccg accttcttgc
     5161 ccttgttccc gcccttcgac ttcggtccct tgccgggccc gtcctcgtcg tcatcgtccg
     5221 gcggcggatc ggtccgcggc gtgacacccg gctcgggctc cggggcgggt cgcggtcgcc
     5281 gcggccgccc gtcgtcgggg cccgctcctc ctccaccctg aggtcctccg actgacggcg
     5341 tgatcccgat gccgatgacg ccgccactgg tcgtcagatc gaggccgagc gtcgggaagg
     5401 gcgcctccgg agggcccccc gcgccgtcac ggacgggcgg cccgccgacg ggcgggtcga
     5461 ggtgctccgg ggcgagggcg ctcggcggcg ggaccgtttg ctggaccaac aggatcagcg
     5521 tcaggagccc gctcaagacg gtgagcgaaa gcatgctcgt gaagaaaagt ggccctcgct
     5581 cccccatcag atgcccctcc ggacggacgt tcgcagggag catctccccc gctcgccgcc
     5641 gatgcaaacg gcctgctcag gaggcacgtc ctgacggcgg tcggcacccg atcgggagaa
     5701 ccggaccaca ccacccatat cggcggaaac tgccaaagcc ttgagcctcc ctcccgacgg
     5761 cggatacgcc ggcttcccta cgatgttcga cgtccggccc acgcttcctt ccctacggct
     5821 cccgcgcgaa gttgcggaac tgcgtgaggt gcgacaggaa cgtcagcttg atgcggtcct
     5881 gtggcccgtt ccggtgcttc gccacgatga tctccgcaag ccccttgacc gcgggatcgt
     5941 cgttgaaggc gtcctcccgg tgcacgaaca tcacgacgtc ggcgtcctgc tcgatggctc
     6001 ccgactcgcg gaggtcggag agctgggggc gcttgtcctg gcgcttctcc gggtcccggt
     6061 tgagctgcga gacggcgatc acggggatct ccagctcctt cgcgagcatc ttcagggatc
     6121 gcgagatctc cgcgatctcc tgctggcggt tctcgacacg gatgtgcgat gacatcagct
     6181 ggaggtagtc gacgatgatg agcccgagtc cctcgcggct cgacctcagg cggcgcgcct
     6241 tcgcgcggat gtccacgatg ttgacgttcc ccgagtcgac gatccacagc ggcgcctggt
     6301 cgaggcgctc cgccgcctcg gcgatgcgcg cccagtcctc ggcgccgatc cggccggcgc
     6361 ggaccttatc ccacgggacg cgtgcctccg cgcagagcag gcgcatcgcg atctccgagc
     6421 gggacatctc gagcgagaac atcgcgacgg gcttgccggt ctcgacgacg acgttgcgag
     6481 cgatgttcgt cacgaacgac gacttgccga cccccgggcg cgcggccacg acgatgaggt
     6541 tgccggggtg gaggccctgc aggagcgagt cgaggtcctg gaaccccgtg ggcaccccgg
     6601 cgaacgcgct gtcgcgctcg tggaggcgct cgagggcctc catcgtctcg ctcacgaggt
     6661 ccgagagcgg gacggcctgc tccttgtccc ggtggcgggc gacgccgtag acgagcccct
     6721 ctgcctcgtc ggcgagcccg acggggtcct cggggacctt gtaggcgcgg tcgacgatcg
     6781 tccacgccgc gtcgatcagc cgacggagca gggcatgctc ggcgacgatc cgcgcgtagt
     6841 gcgaggcgct cgcgggggtc gcgaccgact ccacgaggtg gtggatgtac agcgggccgc
     6901 cgatcgtgtc caggtgcccg agccggcgga gctcctcgac cgtcgtgatc gcgtcgacgg
     6961 gctcgccctg cgcgtacagg cgcagcatcg ccgagtagat cgtgcggtgc gccgaccggt
     7021 agaagtcctc cgccttgacg acctcggtga cgcccgcgat cgcctcgtcg gagaccatca
     7081 tcgacccgag gacggaggcc tcggcctcca ggttgtgcgg cgggacgcgc tcggtgcgga
     7141 gcgcgcgaac gtcgtcagcg gccttcatcg gtctccctcg cctccccagc tcaccccggg
     7201 cgacgctatc cgcgagccgc tgtgggatac aagggcaaca acctgtggat tccctgtgga
     7261 gcgcatgccg gcatcgtaca cccagggaat gacacggatg tcattcgaga gcggcaccgg
     7321 gggccggccg acgggctcgg cgcggtcccg cgacgggctc ggggacgggc tcggggcgct
     7381 caggacaggt tcgggctcgt ctcgaggccg gaaacgtccc gccaggagcc ccatccgaag
     7441 ctgtgcacgc ggttgggtgg cggccgcagg gaacctctgt cagccgtcca gccacgaggc
     7501 gaccctgaga ggacccgccc cgccgacccg cataggccgg cagcacgtga ccggaggccg
     7561 ccgcccaacc gcccgacccc gctgggccgg gcgtcgagcg gctggcaggc tagctgtcct
     7621 gggggacgac ctcgatcgtg gcctgggcgt tgacctctgg gtggaggtgg atggcgacgg
     7681 tgtgcgagcc gacgctgcgg atcggctcgt cgaggtggat ccgcttgcga tcgacctcac
     7741 gcccgacggc cgcggtcagc tcctgggcga tccgctcggc cgtgacgctg ccgaacagcc
     7801 gcccctctcg cccgcctgcg ccgcgatctg gacgggacgg gccgtgagct cggcggcgaa
     7861 cgcctccgcc tccgccttgc ccttcgcgac ccggctcgcg tgcgccgcct tcagccgctc
     7921 ggcgtgccgc accgccccct tcgtggcggc gatcgccatg ccacggggga gcaggtagtt
     7981 ccgcgcgtag ccgtcggcga cctcgacgac gtcgcccggc gtcccgagct tgtcgacctc
     8041 ccgctgcagg atgatcttca tcgtgtcgag tacggcagga gcgcgagctg ccgggccgtc
     8101 ttcaccgcgg ccgccacctc acgctggtgc tgagagcagg tgccggtcac gcggcgcgcg
     8161 cggatcttgc cccggtcgga gatcgaccgg cgcagggtcg ccgtgtcctt gtagtcgatc
     8221 cactcgacct tgtccttgca gaactggcag aacttccggc gctgcttctt ctggaagccg
     8281 tcgtcggcgt cgcgcttccg gcgcccgccg ggaccgctct tcccgccgcc cttgcgatca
     8341 cgagccagtg tcgatccctc cttcgtgcat caggcccctt cgaacggagc ctcctccgac
     8401 gaaccggcgc cgaccgcgac gttcgcgttc cagtcaccgc cgcccccgcc ggagcccgtc
     8461 tgcttctcca ccttcgcggt cgcccagcgc aggctcggac ccaggtcctc cgcctcgatc
     8521 tcgacgaccg tccgcttctg cccttcctgc gtctcgtacg agcgggagcg cagccgtccg
     8581 gcgacgatca cgcggctccc cttcgtcagg gactccgcgg cgttctcgcc gaggctgcgc
     8641 cacgccgtga cggtgaagaa ggaggtctcc tcctcacgcc actggccgct ctggtcctgg
     8701 tagcggcggt tcaccgccac gcggaagttc gccacggcgg ccccgttcgg ggtgtaccgc
     8761 agctcgggat catccgtgag gttgcccatc acgatgacct ggttcccatt ggccatctcc
     8821 atcaccacct tgcctacgcc gtcccgccgc tcgcatggcc cccgaccggc gggaggggaa
     8881 gaacctacgc cgccctcttc accaccttgt gccgcaacac ctggtccgcg agggtcagca
     8941 cgcgctcgag ctccgtcagg tcgcccggct cggctcggaa cttcacgagg acgtagatcc
     9001 cctcggaccg gtggtcgatc tcgtaggcga gcttgcgctt gccccacgtc tcggagctct
     9061 ccacctcacc gccgcgctcg gcgagcaggc tggtggcccg ggacacgacc ccatcgatcg
     9121 ccgcctggtc ggcgtccggg tcgaagatga ccatcacctc gtagtcgcgc atcccacctc
     9181 ctctggtctc gacggccccc tgggggagca ggagtggtcc gaccagaggg tagcacctta
     9241 tcgcccccct ctgacacggt cccctcaggg cgcctcgagc agccaggcca cgaaggtcgc
     9301 cccgcgccac agatcccgca gcgcaccggg cacgtcgccg ccctcgaact cgacgtggcg
     9361 gaagagggag accgtgagcg tccgcgcctc ggggagagcg tgctggaggc ggaggagctc
     9421 ggcgtggggc accgcggcat cgtcggtgga atgcatggcg accaccgggg cgcggaggcc
     9481 cggcgccgcg gccgcgggtg agagccgctc gaggagccgc cgcgcccgcc ccggcagcag
     9541 ctcgatcagc cggggggtcc gctcgggaac ccggttccgc aggagcgcgt ggatcgcggc
     9601 ggccccggcg ggaagggtcg ccgggtcgcg ctgcccgcgg aggacggcgc gcagtgccgg
     9661 ccgctccgcg gggtccacga gggcgagcgc gacctctcgg aggacctcgc cggcgagggg
     9721 gtgaggctcg aacgggatcc gacggtcgcc gacgaccgac gttcgggtcg tcaccgcctg
     9781 caggagaccg cccagctccc agtaggcgcc gaagaccgcg acctgcgcga gacggtcccg
     9841 gatcctcggg tcggccgccg cgacgagcga gaacgacccg ccgaacgaga tcccgaacat
     9901 caccgggcgg ccctccgtcc gcgggtgggc gtcgagcgcc gccgtgaccg cgacgatccg
     9961 gtcgatgtcc cgctcgtcga gccgctccct cgcgagggcc agctcgggga tgaagacgat
    10021 gcgtcccgcg cgcccgaggg cccgagccag ccggacgagt cgctcatcgc ggcgggcgtc
    10081 catcgcggcg cccggaacga ggacgacgag cggtgccctt cccgcaggca ggtaggcgtc
    10141 gcccaccacc ccggcgaccg tgacctcgac gcgcgcgacg tcgtgcgcga acggtcgcgg
    10201 aagcccgagc ccgaccgcct ccgcgacgac cccgacgccg gtcgcccggg cgcggatcgg
    10261 cgggacgaac gcgaggacgg tgcagaacag gagcgcaccg gaaaggatcc tcgcgacgcg
    10321 ccggcgcacc cgctcaggcg tacgagtggc cctcgtcggg gaagacgccg cgctcgacct
    10381 cgtcggcgaa cgcgtggacc gcgcgttcga tgtcccggcg gaggtccgcg tagcgcttga
    10441 cgaaccgcgg cggctcccct tcgccgaggc ccaggaggtc gttgaccacc aggacctgcc
    10501 cgtcggtgtg cggacccgcg ccgatcccga tcgtcgggac ccgcagcgac gtcgtcacgc
    10561 gccgcgcgac gtcgctcggc acgccctcga ggacgagcga gaagatgccg gccttctcga
    10621 gcgtgagcgc gtcggagagc agccggtcgg cgtcttcctc ggatcggccc tggacccggt
    10681 agccgcccat cgagtggacg gactgcggcg tgagaccgag gtgccccatc accgggatgc
    10741 cgcgcgaggt gagcgcctcg gccagctgga agagcggacc ctcgagcttc acggcgtgca
    10801 tcccgccttc cttgaggaac cggccggcgt tgcggacgcc ttcctccacc gaggcctggt
    10861 aggacatgaa cggcatgtcg ccgaccagca gcgcgtgctc cgccccgcgc gcgaccgcct
    10921 tggcgtggtg gagcatctcc tccatcgtca ccggcagcgt cgtctcgtag ccgaggacga
    10981 cgttcgcgag cgagtcgccg acgaggatca ccgggatccc ggcacggtcg aggatccgag
    11041 cggtcgggtg gtcgtacgcg gtgagcatcg cgaagcgccg cccctgcgcc ttccacgcgg
    11101 cgaggtcgtg gatcgtggtc atggctcgcg cctccttccg aagtgcacgg cgtgcggggt
    11161 caggctacca ccgctcagcc ggaggggaac tcgagcgtga gcgcgtgcat gtcggcgctc
    11221 acgctcgagt tcccctccgg ctgagcggtg gtag
//
LOCUS       DATLGG010000234         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_230521, whole genome shotgun sequence.
ACCESSION   DATLGG010000234 DATLGG010000000
VERSION     DATLGG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230521"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1189)
                     /locus_tag="VM638_06730"
     CDS             complement(212..1189)
                     /locus_tag="VM638_06730"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HVM12150.1"
                     /translation="MAAVPPLDELNTMPPDAFAAAVAPLFEGAPRFLHRLAEEGPFST
                     DSELFDAARAVARGLPEEAQVELLNAHPRIGADPASVSSLSYAEQGYDGEGHEDRAWV
                     DDELRALNEAYESHFGFRFVIFVAGRPRIDIIPIIERALHADRDEELRRGLDDVVLIA
                     ADRHATLQGPAPLREELREAIALEISRWMIGELDRNGLIRAAHRLIDEDVRSPALLDL
                     SLANEHEEPNLDRPVARLMSEIGLAGWDAGQAGQLLALHAAASILGEVSQPIDGARRI
                     ASVASDPRFAELVSRWEADPGARASTDEEIRRAATELFGPHDQGSWHEA"
     gene            complement(1195..1569)
                     /gene="uraH"
                     /locus_tag="VM638_06735"
     CDS             complement(1195..1569)
                     /gene="uraH"
                     /locus_tag="VM638_06735"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HVM12151.1"
                     /translation="MEVPQRRISMADARPTISTHVLDLATGDPAAGVSVALFFLADDG
                     TPELLAEAETDDDGRIRDLLAGSPLREGDYQLAFDVGGYADDPDAFFQSAALALRISD
                     TDRSYHVPLLLGPFGMTTYRGS"
     gene            1613..2026
                     /locus_tag="VM638_06740"
     CDS             1613..2026
                     /locus_tag="VM638_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HVM12152.1"
                     /translation="MADLKITVGDLEFAARWEDAAPLTREALRPWLPIRSKLIHCKWS
                     GESTWIPFGEERPDVGYENHNTHPAPGDLLIYTGDISECEILFPYGACSFSSRVGPLA
                     GNHFATLTAGREHLAEMGRRVLWEGAQEIEISEGS"
     gene            2023..>2332
                     /locus_tag="VM638_06745"
     CDS             2023..>2332
                     /locus_tag="VM638_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HVM12153.1"
                     /translation="MTRRLITGGRVVTSSGTSKLDVAIRDGRIEAVGPGLPRDAAEVI
                     DATGLLVLPGTIDVHTHVRLSDAEHPRRFEQDLAAAAAGGTTTVLTFNNPGTGISDRG
                     A"
BASE COUNT          363 a          801 c          835 g          333 t
ORIGIN      
        1 gccacaggcg gcgaggatga tggcgagcga tgccgcgggc aacagcgcga cgcgtgggtt
       61 gcttcggcgc aaggacgaaa ggttcacggg ttcccctagc aggcatgaaa cggcacgccc
      121 agtggcgtgc cgcctcggag cctaggtgat gaggcgagcg tggcgcaaac ggccgatggt
      181 ccgcttggcc ggcaggaccc gagctcggcc ctcatgcctc atgccaactc ccctgatcgt
      241 gcggtccgaa cagctccgtg gcggccctgc ggatctcctc gtcggtggaa gctcgggcgc
      301 cgggatccgc ctcccagcgc gacacgagct ccgcgaagcg tgggtcggag gcgaccgatg
      361 cgatgcgccg ggcgccgtcg atcggctgac tgacctcgcc caggatcgag gcagccgcat
      421 ggagagccag cagctgcccg gcctgcccgg cgtcccatcc ggcgagcccg atctccgaca
      481 tgagacgcgc gaccggacgg tcgaggttcg gctcctcgtg ctcgttcgcc agtgacaggt
      541 cgagcagggc cggcgaccgc acgtcctcgt cgatgagccg atgcgccgcc cggatgagcc
      601 cgttgcgatc cagctcgccg atcatccagc gcgagatctc gagcgcgatc gcctctcgca
      661 gctcctcgcg aagcggggcc ggtccctgga gggtcgcatg gcgatccgcc gcgatgagga
      721 cgacgtcgtc gagaccccgc cggagctcct cgtcccgatc cgcgtggagc gcccgctcga
      781 tgatcggaat gatgtcgatg cggggccgcc cggcgacgaa gatcacgaaa cggaacccga
      841 agtgcgactc gtatgcctcg ttcagggccc gcagctcgtc gtcgacccag gcacggtctt
      901 cgtggccctc cccgtcataa ccctgttcgg cgtacgagag ggaggagacg ctggcggggt
      961 cggcaccgat gcgcggatgg gcattcagca gctcgacctg ggcctcctcc ggaagcccgc
     1021 gcgcgacggc gcgggccgcg tcgaacagct cgctgtccgt cgagaacggc ccctcctcgg
     1081 ccaggcgatg gaggaagcgg ggtgcgccct cgaacagcgg cgcgacggcg gccgcgaatg
     1141 cgtcgggcgg catcgtgttg agctcgtcga ggggcggaac cgcggccatc tgcgtcagct
     1201 tccgcggtac gtcgtcatgc cgaatggacc cagcagcagc ggcacgtgat acgagcggtc
     1261 ggtgtcgctg atccgcaggg cgagggcggc gctctggaag aacgcgtcgg ggtcatcggc
     1321 atagccgccg acgtcgaacg ccagctggta gtcgccctcg cggaggggcg agccggcgag
     1381 gagatcgcgg atgcggccgt cgtcgtcggt ctcggcctcg gcgagcagtt cgggcgttcc
     1441 gtcgtcagcc aggaagaaga gcgccacgct cacccccgcg gcgggatcgc cggtggcgag
     1501 gtccaggacg tgggtgctga tggtggggcg cgcgtcggcc atcgagatcc tccgctgtgg
     1561 gacctccatc atgaccgatg ctcgccgccg ttcgtggcgt atgctcgtcg gcatggctga
     1621 cctgaagatc acggtgggcg acctggagtt cgccgcacgc tgggaggacg cggcgcccct
     1681 cacccgcgag gcgctgcgac cctggctgcc gatccgctcg aagctcatcc actgcaagtg
     1741 gagcggcgag tcgacgtgga tcccgttcgg cgaggagcgg ccggacgttg gctacgagaa
     1801 ccacaacacg caccctgccc ccggcgacct gctcatctac accggtgaca tcagcgagtg
     1861 cgagatcctc tttccctacg gtgcgtgcag cttcagctcg cgcgtcggtc cgttggcggg
     1921 gaaccacttc gcgacgctga ccgccggacg agagcatctc gccgagatgg gccgtcgtgt
     1981 cctgtgggag ggcgcgcagg agatcgagat cagcgaaggg tcgtgacccg tcggctcatc
     2041 acggggggac gcgtcgtcac ctcgtccggc acctcgaagc tcgacgttgc catccgcgac
     2101 ggacggatcg aggcggtcgg accgggcctg cccagggacg cggccgaggt catcgacgcg
     2161 accggcctgc tcgtgctgcc cggcacgatc gacgtccaca cccacgtccg cctctccgac
     2221 gcggagcacc cccggcggtt cgagcaggac ctcgccgccg cagccgcggg aggcaccacc
     2281 accgtgctga ccttcaacaa ccccgggacg gggatcagcg accgaggagc cc
//
LOCUS       DATLGG010000235         7639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_93763, whole genome shotgun sequence.
ACCESSION   DATLGG010000235 DATLGG010000000
VERSION     DATLGG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93763"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VM638_06750"
     CDS             <1..375
                     /locus_tag="VM638_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12154.1"
                     /translation="RSRRRQDLRQGERALALPEVLSSAAPPELVSIGEPTLVTALGVG
                     LLVAGAIVGLGRGTRPAVERPDEAPAPLAAPWRRLAERGGALRERTEEALVGPVLAAL
                     LLLGAVAIAVRLVLVAAAQGFL"
     gene            421..2619
                     /locus_tag="VM638_06755"
     CDS             421..2619
                     /locus_tag="VM638_06755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVM12155.1"
                     /translation="MAVRALPRKRTGKVARRSRRIRTKRGFLRRTWWLWAPPVALATA
                     LALALTYVYVRLPLPKEPAAAQTTFLYDANGKMLTTVRGEVDRTVIPLKTMPLHLRQA
                     VIAAEDEDFYSHPGISLPAIARAAWANLRGRGIQQGASTVSQQYVRNAFRDELGYDRR
                     TGFDRTIARKLKEALLAIKLERKISKNQILERYLNTIYFGRSAYGAEAAARTYFGTKA
                     ENLTLLQSATLVGLIPRPVGTDPFEYPERALQERNRVLRRMAEAGFISDSTSRELQEK
                     PLKVHNDKQTRRERNVTRGPAAYFVDYAKRTLQQKYGESQTFTGGLRVTTTIDMKMQR
                     FAEKAVSTHLGDRGDPDAALVAIDNRTGEVRAMIGGKNFRKAKCNLATARGGGRNCGT
                     GRQTGSSFKMFTLAAAVDKGISLRSVFSGPGRIVMKDNPECGEWSTRGVGNYADSGSG
                     TMDLIGATAKSVNTIYAQLVAEVGPQRVVEMAKRLGIKSKLSAVCSITLGVHPVSPLE
                     MTTSFATIASGGVRRDPTPLRRVETSEGEVLYRNTKDGKRVMKENDAWQVAKALRSVV
                     TGGTGTAAALPNVEIIGKTGTSQNHANAYFCGSSRILTTCVWVGHSEGNIPMSGVTGG
                     SYPARIFHSFMAPAHEGKSVPGFPEPTLTGKTVKGKNVSTGDSTAAPEPTQRKPKPKP
                     KQTVQPSPKPEPEPEPEPTCGAPPNPPCPEPTTGTGSSGSGTSSTSDPGG"
     gene            complement(2620..3246)
                     /locus_tag="VM638_06760"
     CDS             complement(2620..3246)
                     /locus_tag="VM638_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM12156.1"
                     /translation="MPVTVVVADDHSMVRQGLRRTLEETPDIEVVGEASTGAQAVDVV
                     ARTSPDMVLLDVRMPDMDGIEATGEITSRFPGVNVVMLTGSDDRRLVLAAVRAGARGY
                     ILKTREAETLAGTLRLVAEGNLVIDPEVAAAVGEELAGGAPAHGLTDREMEILKLVAD
                     GLTNREIGARLYVSADTVKTHLEHVFQKLGASDRTAAVAQALRRGLIE"
     gene            complement(3312..4511)
                     /locus_tag="VM638_06765"
     CDS             complement(3312..4511)
                     /locus_tag="VM638_06765"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HVM12157.1"
                     /translation="MTTRTAPGEDEATGQAERLRIRRGILYCLAVFVAARVGLSIVAA
                     LGVALIDPLEPTTDSGWRTAPFAPGWHSIVTAWERFDALWFLRISDVGYVQPKSAVFF
                     PLYPLAIRAVSPLLGGHPLAAAIVVGNAAYIGGLIATFFLTAGEWGERVARRTILYLA
                     IFPTAFFLLAPYSEPLFLLLVTTSLWGARRGRWAIAGIAAALAAMTRNLGIVLVLPLA
                     FEAWHQHRQQGTRLFPTVLWSLLPAAGLLAYLGYWEVAGGSWRAPFELQEEWERAIAF
                     PPWTVIQATYQAFRWIGVYPGGYHLLDWLIVAPMLVAGVWVARRARPIFSLYTWASLL
                     VPLSFVFAPRPFMSLPRFLLPIVPLFWAAAVVAERRPWLHEAVVVASSALLGVMTVLF
                     VSWYYVF"
     gene            complement(4508..5671)
                     /locus_tag="VM638_06770"
     CDS             complement(4508..5671)
                     /locus_tag="VM638_06770"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HVM12158.1"
                     /translation="MRPRRSLATVLAATGITLLVGLAIKWPCVTGDWADGRQYRRLCY
                     TDIVPLYTARGIDDGRVPYLEARNEYPVLTGAAMWAAGVPARSYASFFGWTAAMLAAA
                     AFATAWALHRLVGRRALFFAAAPTLAIYGFMNWDLLAVAPATLAVLLYPAGRYLASGA
                     LLGLGAAAKLYPALFVLPLGLRRLQRGDRTGAIRLAAAALAVWSALNLIVAIAAPDGW
                     AEFFRFSAERPADWDSLWLLAGHHLGWPRDTGTVNAAAALSFLGLAALTWWRAARRRP
                     GFPAWTFAFPLLVLFLVTSKVYSPQFSLWLLPWFALALPHLGLFVVFSAAEVAVFVTR
                     FTWFANVEAHPFPAPEFQAAIVVRAIVLLACVAAWVRRPAPEVTPETRSEAAR"
     gene            5712..>7639
                     /locus_tag="VM638_06775"
     CDS             5712..>7639
                     /locus_tag="VM638_06775"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HVM12159.1"
                     /translation="MERGGAGRAGIAGCLLALAVLLPACGAGKDYSFPDVRIDATILP
                     DGSLDLVERRTFRFDGEFSVAFFSIEHPATLIQDFRVTRDGRPLPVTTVAATWERFEA
                     EWPSPARDEDVTFTVAYRARCAVEVFGDAAHLLWQFVGRGWEKPTDRVLVTLRVPGRA
                     TEIPERPLLPCRLATDPPPDRRLETVPLARGDVRAWGHGPYSGEVAFVDPQTVTFEVR
                     DLRPATFVEGSVLMPPEVVPLAYQLSLPARDRILREEAGLAAEANRLRARSARARTTA
                     VALLLLLPLLVLGLVALSRRRDRVAGVPPYLEEPPDETHPAELAHAWATLEKANAGER
                     AYRTQLLHLARQGTIEIAPIGRVTKPSDFTLRRRKEAAPGLDQEFMEFLFPDGQGSVR
                     LKELTATGSRGAELKEWSKELASTATGKLAKVVASRTRVESWLLAGLAAAVAVWSVRS
                     AGFGPADVVLSAAVGLLAGGLVRIVPALLRDPALALVWAVIAGVIGVPSLIPLWLALS
                     EGGTLRPTLTHAAVALAFALWPLGLRFMREHLPPTLRRRIARWRAFRRYLKDFSSLPE
                     APTMAVTIWERYMVYAVALGVAAEVERQVRALVPAEELGSPFAGAPAGFDGTDAYRSF
                     ATTRIPSRAYAPLAAGSGS"
BASE COUNT         1161 a         2780 c         2712 g          986 t
ORIGIN      
        1 cggagccgcc gacgacagga cctccggcaa ggcgagcgcg cgctcgcctt gccggaggtc
       61 ctgtcgtcgg cggctccgcc ggagctcgtt tcgatcggcg agccgaccct cgtgacggcc
      121 ctcggcgtcg gcctgctcgt cgccggagcc atcgtcgggc tcgggcgtgg gacgaggcca
      181 gcggtcgagc gaccggacga ggctcccgca ccgctcgcag cgccgtggcg gcgcctggcc
      241 gagcgcggtg gcgccctccg ggagcggacc gaggaagcgc tggtcggccc cgtcctcgcc
      301 gcgctcctcc tgctcggggc ggtggcgatc gccgtgcgac tcgtcctcgt cgcggccgcg
      361 cagggattcc tctagcgcgc gcggaatgcg ccgttggggc gcgggtacac ttgctctcca
      421 gtggctgttc gagcacttcc ccggaagcgg accggcaagg tcgcccgtcg atctcgccgg
      481 atccggacga agcgcggctt cctgcgccgc acgtggtggc tgtgggcgcc gcccgtcgcg
      541 ctggcgaccg cactcgccct cgcgctgacc tacgtctacg tgcgcctgcc cctgccgaag
      601 gagcctgccg cggcacagac gaccttcctt tacgacgcga acggcaagat gctgaccacg
      661 gtccgcggcg aggtggaccg gacggtgatc ccgctgaaga cgatgcccct gcacctccgt
      721 caggccgtga tcgccgccga ggacgaggac ttctactccc acccggggat cagccttccg
      781 gcgatcgcgc gcgcggcgtg ggcgaacctg cggggtcggg ggatccaaca gggggcctcg
      841 accgtgtccc agcagtacgt ccgcaacgcc ttccgtgacg agctgggcta cgaccggcgg
      901 accggcttcg accggacgat cgcccgcaag ctcaaggagg cgctcctcgc gatcaagctc
      961 gagcggaaga tctcgaagaa ccagatcctc gagcgctacc tcaacacgat ctacttcggt
     1021 cgaagcgcct acggggccga ggcggccgcg cgcacttact tcggcacgaa ggccgagaac
     1081 ctgaccctcc tgcagtccgc gacgctcgtc ggcctcatcc cccggcccgt ggggacggac
     1141 cccttcgagt accccgagcg cgcgctgcag gagcggaacc gggtcctgcg gcgcatggcc
     1201 gaggccggct tcatctccga cagcacctcg cgcgagctcc aggagaagcc gctcaaggtc
     1261 cacaacgaca agcagacccg tcgggaacgg aacgtgaccc gggggccggc cgcctacttc
     1321 gtggattacg cgaagcgcac cctccagcag aagtacggcg agagccagac cttcaccggc
     1381 gggctgcgtg tcaccacgac gatcgacatg aagatgcagc ggttcgcgga gaaggccgtc
     1441 agcacccacc tcggcgacag gggggatccc gacgcggcgc tcgtcgcgat cgacaaccgc
     1501 accggagagg tccgggcgat gatcggcggc aagaacttcc ggaaggcgaa gtgcaacctc
     1561 gcgaccgccc gcggcggcgg gcggaactgc ggcaccggcc gtcagaccgg gtcgtcgttc
     1621 aagatgttca ccctcgcggc cgctgtcgac aaggggatct cgctgcgctc ggtgttctcg
     1681 gggccgggcc ggatcgtcat gaaggacaac ccggagtgcg gcgagtggtc gacgcggggc
     1741 gtcggcaact acgccgactc cggctccgga acgatggatc tcatcggcgc gacggcgaag
     1801 tccgtcaaca cgatctacgc ccagctcgtg gccgaggtcg gcccgcagcg cgtcgtggag
     1861 atggcgaagc ggctcgggat caagagcaag ctctccgccg tgtgttcgat caccctcggc
     1921 gtccacccgg tgtcaccgct cgagatgacg acctcgttcg cgacgatcgc ctcgggcggg
     1981 gtccggcgcg acccgacccc gctcaggagg gtcgagacgt ccgaggggga ggttctctac
     2041 cgcaacacga aggacggcaa gcgcgtcatg aaggagaacg acgcatggca ggtggcgaag
     2101 gcgctgcggt ccgtggtgac gggcgggacg ggcaccgcgg ccgcgctgcc gaacgtcgag
     2161 atcatcggca agacgggtac ctcgcagaac cacgcgaacg cgtacttctg cggaagctct
     2221 cgcatcctca cgacctgcgt ctgggtcggc cacagcgagg gcaacatccc gatgtcgggc
     2281 gtcaccggtg gctcctaccc ggcgcggatc ttccactcct tcatggcgcc ggcgcacgag
     2341 ggcaagagcg tcccgggctt ccccgagccg accctcaccg ggaagaccgt caagggcaag
     2401 aacgtctcga ccggcgactc caccgccgcg cccgagccaa cacagcggaa gccgaagccg
     2461 aagccgaagc agacggtcca gccctctccg aaacccgagc cggagccgga acccgagccg
     2521 acgtgcggcg ccccgccgaa ccccccgtgc ccggagccca cgaccggaac cggctcctcg
     2581 gggtccggca cgagctcgac ctccgacccc gggggctagc tactcgatca gcccgcgtcg
     2641 cagcgcctgg gcgaccgcgg ccgtgcggtc cgaggctccg agcttctgga agacgtgctc
     2701 gaggtgggtc ttcaccgtgt cggcggagac gtagagccgc gctccgatct cgcggttggt
     2761 cagcccgtcg gcgacgagct tcaggatctc catctcccgg tcggtgaggc cgtgcgccgg
     2821 cgcgccgccg gccagctcct cgccgaccgc ggcggcgacc tccgggtcga tcacgaggtt
     2881 gccctccgcg acgagccgta gcgtgcccgc cagcgtctcc gcctcccggg tcttcaggat
     2941 gtatccgcgt gccccggccc gcaccgcggc gagcaccagg cgccggtcgt cggagcccgt
     3001 gagcatcacg acgttcacgc ccgggaaccg cgatgtgatc tctccggtcg cctcgatgcc
     3061 gtccatgtcc ggcatccgaa cgtcgagcag gaccatgtcc ggggaggttc gcgccacgac
     3121 gtcgacggct tgggcgccgg tcgaggcctc cccgacgacc tcgatgtccg gggtctcctc
     3181 gagcgtgcgg cgaaggcctt ggcggaccat cgagtgatcg tcggcgacca ccacggtgac
     3241 gggcacgggc aacctcctgt cgcgggtgcg ccgatcgtag tagggcgatc ggccggaaag
     3301 caagtcgagc atcagaacac gtagtaccag gagacgaaca gcacggtcat gacgccgagg
     3361 agcgcggacg aggcgaccac gacggcctcg tgcagccagg gccgccgttc cgccaccacc
     3421 gccgccgccc agaacagtgg gacgatcggg agcaggaagc gcggcagcga catgaacggc
     3481 cgaggcgcga agacgaagga cagcggcacg agcaggctcg cccacgtgta cagcgagaag
     3541 atcgggcgtg cccgccgcgc cacccagacg ccggcgacga gcatcggcgc gacgatcagc
     3601 cagtcgagca agtggtagcc gcctgggtag acgccgatcc agcggaacgc ctggtacgtc
     3661 gcctggatca cggtccacgg tgggaacgcg atcgcccgtt cccactcctc ctgaagctcg
     3721 aacggcgccc gccagctgcc gcccgccacc tcccagtagc cgaggtacgc gagcagtcct
     3781 gccgccggca gcaacgacca caggaccgtc gggaacagcc gtgtcccctg ttgccggtgc
     3841 tggtgccatg cctcgaaggc gagcggcagg acgaggacga tcccgaggtt gcgtgtcatc
     3901 gccgcgaggg ccgcggcgat gcccgcgatc gcccaccggc cgcgccgcgc cccccacagg
     3961 gaggtcgtca ccagcagcag gaacagcggc tccgaatacg gcgcgaggag gaagaacgcc
     4021 gtcgggaaga tcgcgaggta caggatcgtc cgccgcgcga cccgctcgcc ccactcgccg
     4081 gcggtgagga agaacgtcgc gatcagcccg ccgatgtacg ccgcgttgcc gacgacgatc
     4141 gcggcggcga gcggatgtcc cccgaggagg ggggacaccg cccggatcgc gagcgggtag
     4201 agggggaaga agaccgcgct cttcggctgc acgtagccca cgtcggagat gcgcaggaac
     4261 cacagggcgt cgaaccgctc ccacgccgtg acgatgctgt gccacccggg cgcgaacggc
     4321 gccgtgcgcc agcccgagtc ggtggtgggt tccagcggat cgatcagggc gacaccgagc
     4381 gccgcgacga tcgagagtcc gacgcgagcc gcgacgaaca cggcgaggca gtacaggatc
     4441 ccgcggcgga tccgcaggcg ctcggcctgc ccggtcgcct catcctcccc aggtgcggtt
     4501 cgcgtggtca ccgcgccgcc tccgacctcg tctccggcgt gacctccggc gccggccgcc
     4561 ggacccatgc cgcgacgcac gcgaggagca cgatcgcccg gacgacgatc gccgcctgga
     4621 actccggtgc cgggaaggga tgcgcctcca cgttcgcgaa ccacgtgaac cgcgtcacga
     4681 agaccgcgac ctccgcggcg gagaacacca cgaagaggcc gaggtggggg agcgcgagcg
     4741 cgaaccaggg gaggagccag aggctgaact gcggcgagta gaccttgctc gtcacgagga
     4801 agagcaccag gagggggaac gcgaacgtcc acgccgggaa ccccgggcgg cgccgcgccg
     4861 cgcgccacca cgtgagcgcc gcgagcccga ggaaggacag ggcggccgcc gcgttgacgg
     4921 ttccggtgtc gcggggccaa ccgaggtggt gccccgcgag gagccacagg ctgtcccagt
     4981 ccgccggccg ctctgcgctg aagcggaaga actccgccca cccgtcgggc gcggcgatcg
     5041 cgacgatgag gttcaaggcg gaccagacgg cgagcgcggc ggccgcgagc cggatcgccc
     5101 ccgtccggtc gccgcgctgg agccgccgca ggccgagggg cagcacgaac agcgccgggt
     5161 acagcttcgc cgcggcgccg aggccgagca gggcgccgct cgccaggtag cggcccgccg
     5221 ggtacagcag gaccgccagc gtcgcgggcg ccaccgcgag cagatcccag ttcatgaacc
     5281 cgtagatcgc cagcgtcggc gcggcggcga agaacaacgc gcgccgtccg acgaggcggt
     5341 gcagcgccca cgcggtcgcg aaggctgcgg cggcgagcat cgcggccgtc cacccgaaga
     5401 aggaggcgta cgagcgagcc ggcacccccg cggcccacat cgccgccccg gtcagcacgg
     5461 gatactcgtt cctcgcttcg aggtagggga cgcgcccgtc gtcgatcccg cgagccgtgt
     5521 acagggggac gatgtcggtg tagcagaggc gccggtactg acgaccgtcg gcccagtcgc
     5581 cggtgacgca gggccacttg atcgccaggc cgacgaggag cgtgatgccc gtcgcggcga
     5641 gcaccgtcgc gaggctccgt cgagggcgca tcccgcgatt atcgggcatc gcgcggcgta
     5701 cactccggcg cgtggaacgg gggggagcgg ggcgtgccgg gatcgcgggc tgcctcctcg
     5761 cgctcgcggt gctgcttccc gcgtgcgggg cggggaagga ctactcgttc ccggacgtcc
     5821 ggatcgacgc gacgatcctg ccggacggct cgctcgacct cgtggagcgc cgcaccttcc
     5881 gcttcgacgg ggagttctcc gtcgcgttct tctcgatcga gcatcccgcg accctcatcc
     5941 aagacttccg tgtcacccgc gatggccgcc ccctgccggt gacgacggtc gccgcgacct
     6001 gggagcggtt cgaggccgaa tggccatctc cggcccgcga cgaggacgtc accttcacgg
     6061 tggcgtatcg cgcccggtgc gcggtcgagg tgttcggcga cgccgcgcac ctgctgtggc
     6121 agttcgtcgg gcgcggatgg gagaagccga ccgaccgggt cctcgtgacc ctgcgcgtgc
     6181 cggggcgagc gaccgagatc ccggagcgtc cgctgctgcc atgccgcctc gccaccgatc
     6241 cgccgcccga ccgacggctc gagacggtgc cgctcgcgcg cggggacgtt cgcgcctggg
     6301 gccacgggcc gtacagcggc gaggtcgcct tcgtcgatcc gcagacggtg acgttcgagg
     6361 tccgcgacct ccgtccggcg accttcgtcg aaggatcggt cctgatgcca cccgaggtcg
     6421 tcccgctcgc ctatcagctg tcgctcccgg cccgcgaccg gatcctgcgg gaggaggccg
     6481 gcctcgcggc cgaggcgaac cgtctccggg cccgttccgc tcgcgcgcgc accaccgccg
     6541 tcgccctcct gctcctgctg cccctgctgg ttctcgggct cgtcgccctg tcccgccgcc
     6601 gcgatcgcgt cgcgggcgtt ccgccctacc tcgaagagcc gccggacgaa acccaccccg
     6661 ccgagctcgc gcacgcgtgg gcgacgctcg agaaggcgaa cgccggtgag cgtgcttacc
     6721 gcacgcaact gctgcacctc gcgaggcagg gcacgatcga gatcgccccg atcggacgcg
     6781 tcacgaagcc gagcgacttc acgctgcgcc gccgcaagga ggcggcgccc ggcctcgacc
     6841 aggagttcat ggagttcctc ttcccggacg gtcagggctc cgtccgcctc aaggagctga
     6901 ccgcgacggg atcgcgcggc gcggagctga aggagtggtc gaaggagctc gcttcgacgg
     6961 ccaccgggaa gctggcgaag gtcgttgcga gtcgcacccg cgtggagtcg tggctgcttg
     7021 ccgggctcgc cgccgcggtc gcggtctggt ccgtccgctc ggcgggcttc gggcccgcgg
     7081 acgtcgttct ctccgccgcc gtcggcctgc tcgccggcgg cctcgttcgc atcgtccccg
     7141 cgctcctgcg cgacccggcg ctcgctctgg tgtgggccgt catcgccggc gtcatcggtg
     7201 tcccgagcct gatcccgctg tggctcgcgc tgtcggaggg gggcaccctg cgcccgacgc
     7261 tcacccacgc ggccgtcgcc ctcgccttcg cgctgtggcc gctcggtctc cggttcatgc
     7321 gcgagcacct cccgccgacg cttcgccggc ggatcgcgcg gtggcgcgcg ttccgtcggt
     7381 acctgaagga cttctcgtcc ctgccggagg cgccgacgat ggccgtgacg atctgggagc
     7441 ggtacatggt gtatgccgtc gcgctcggcg tcgccgccga ggtcgagcgc caggtccgcg
     7501 ctctcgtccc cgcggaggag ctcggatcgc cgttcgcggg cgctcctgcc ggcttcgacg
     7561 gcacggacgc ctaccgctcg ttcgcgacga cccggatccc gagccgcgcc tacgcgccgc
     7621 tcgccgcggg gagcgggtc
//
LOCUS       DATLGG010000236         6441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_61237, whole genome shotgun sequence.
ACCESSION   DATLGG010000236 DATLGG010000000
VERSION     DATLGG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61237"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1698
                     /locus_tag="VM638_06780"
     CDS             49..1698
                     /locus_tag="VM638_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVM12160.1"
                     /translation="MPGPGADWSAHQLAEFLAAVSSFTDQGSAKQGAVERAAEAFEAE
                     VGALIEEGDLSAVVGFPPDAVPTDVLLDLGRDSPGETDLRTLGTSQVLSVPLGDRDDS
                     RFLVARAGSAEFSVEERNLLRAMGRVLELTLGMLERQRLLEHLSRIQRSISHRAPLRE
                     VLDAVTTGASDLLGVEVAGLRLIDPGDPAFVLTASAAGVDADAMKAVLRMPLGQGAGG
                     RAISEDRLVIIEDYASAPGMVPHFASRALQVAMAAPVHENGVVVGSLVVASYKPGRRF
                     SGEEQDALLALAEHASLALTDAKIVEEMREAQRSKDLFLAMVSHELKTPLTVIMGGLR
                     TLEVHGEDLPAELRHEILAAGWHRGRELERLINRLLQGARAELVPRVEDVDLFELVAE
                     GVRGLEHSRRVRFDVPTDVAIRVDRPAVLEVLGILLENAVSHSGPGTDVWLDARVERR
                     ELVLGVANEGQLPEGVDPETLFRPFQRGDDTRSPGVGLGLYIASRVAESIDGKVSVET
                     DGGRVRFVLRVPVFEVGAGLPDARPGPRDDIRAPAPMQPGR"
     gene            complement(1640..2323)
                     /locus_tag="VM638_06785"
     CDS             complement(1640..2323)
                     /locus_tag="VM638_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM12161.1"
                     /translation="MGRAAAEGLDRLYETARALGEETRFRIYRRIWLSPLPEGVAELA
                     AAFSLHPNAVRQHLTRLEQAGLIVSALERPGGAGRPRRVYAPSPEPFELGPDAAMRPL
                     VSLMAEAVDALPGDRRKLTRFGEDWARSWTSKRKRGNGRAPRDPRARAELLSRELARW
                     GWRPSAINENGGMRITTGRCLFHGGAPGENGRSCSLEEGLLRGLTEAMLNGRAASVQV
                     RGCRLEVRV"
     gene            2516..2788
                     /locus_tag="VM638_06790"
     CDS             2516..2788
                     /locus_tag="VM638_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVM12162.1"
                     /translation="MAVSDWEMGWQYRASCRGEDAEMFFAPNYFERKDEKDAREVRAK
                     AICADCPVRVECLEYALRSREPHGIWGGLNELERRALIRQRERRAG"
     gene            2793..3251
                     /locus_tag="VM638_06795"
     CDS             2793..3251
                     /locus_tag="VM638_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVM12163.1"
                     /translation="MGFDDRLDELGIELPPAAAPVASYVPVTTAAGLAFVAGQVPLEG
                     GTPIATGRVGEAVSVEEGNALARRCALQALAALRQELGSLDRIRRIAKVTVWVASQVG
                     FTAHPQVANGASDVLVEIFGDVGKHARAAVGAPSLPLGVPVEVELVVELT"
     gene            complement(3257..4324)
                     /locus_tag="VM638_06800"
     CDS             complement(3257..4324)
                     /locus_tag="VM638_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HVM12164.1"
                     /translation="MTAAEISEAAAREAGLRYVSDAAPGIRRRRAGRGFVYLGPDGER
                     IDDGATLARIRGLAIPPAWTDVWICATPGGHIQATGRDGRGRKQYRYHEQWRRVRDET
                     KYASLIEFGEALSAIRARVRRHLALPGLPREKVLATVVRLLDTTLARVGNPEYARDNQ
                     SFGLTTLRDRHVDVGREEVLFEFIGKGGKKHRLSVEDPRLAAIVRACRDLPGYDLFQY
                     VADDGTPVAVGSADVNAYLREIAANDVTAKHFRTWGGTVLATQVLRRREPPRSERAAK
                     REVAAAIKEVAAELRNTPAVCRKCYVHPDVIEAYTEGVLFEQLKRSRAAWASRLRELR
                     REEIEVLSLLRRRASARSRAA"
     gene            complement(4374..6233)
                     /gene="selB"
                     /locus_tag="VM638_06805"
     CDS             complement(4374..6233)
                     /gene="selB"
                     /locus_tag="VM638_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HVM12165.1"
                     /translation="MSGALQVVATAGHVDHGKSSLVAALTGMDPDRWEEEKRRGLTID
                     LGFAWTTLPSGREIGFVDVPGHERFVRNMLAGVGPVRLVLFVVAADEGWKPQSEEHLG
                     IVDLLGAGAAVVALTKADLAEGGLVEARLREIGERLAGTALEGAPVVPCSAATGAGIE
                     DLRSALDELARTAPPEPETDRPRLHVDRSFSVAGAGTVVTGTLAGGALSVGDEVAIFP
                     SGRTVRIRGLQSHRRTMEEARSVSRVAASLAGTPRDAVSRGDVVARPQQWRPTSRLEV
                     RLRPIRSLRDPLTARGAYKLYLGAAERDVRLHLYDRRELAPGTEGFARLKLDEPVIAA
                     VGDPFVLRESGRRATVAGGEVLDADPPHRPGAAATRLEARRHLAPHDLAEVLVAERTA
                     VRSTDLPPLTGSSPGAIRAAERVGAWWVRDEALEETRDALTRLLAERHAGNPLVPGFP
                     MDEAAAELAGTLGTLRPALEAGLLPALLAHLEARRDVVREGTLLRLPGHQVGLGDREE
                     EAQKLVAAVASGEPTPPDAAELGRLGFDRDLVDAACATGLLVRVSQELVVTPAFAARA
                     EELARRGAATPSGLTVSRFRELLGTTRKYALPLLGWLDARGVTRREGDVRRAG"
     gene            complement(6230..>6441)
                     /locus_tag="VM638_06810"
     CDS             complement(6230..>6441)
                     /locus_tag="VM638_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12166.1"
                     /translation="TRWVGIAHPVRLAARDPGRLAARLRQGTPPVFCRVADDAILLDL
                     RTVMPGEDDLLIRALRYALREDPAP"
BASE COUNT          851 a         2352 c         2355 g          883 t
ORIGIN      
        1 atcagcgggt tccacaacca gaccctcgtc gtcctggcgg tgagctgagt gccgggaccc
       61 ggcgccgact ggtccgcgca ccagctcgcc gagttcctcg ccgccgtctc gtcgttcacg
      121 gaccagggca gcgccaagca gggggcggtc gagcgcgcgg ccgaggcgtt cgaagccgag
      181 gtcggtgcgc tgatcgagga gggggatctc tcggcggtcg tcggcttccc gcccgacgcg
      241 gtgccgacgg acgttctcct cgatctgggg cgggactcgc ccggggagac cgacctccgg
      301 acgctcggca cgagccaggt gctttcggtc cccctcggcg accgcgacga cagccgcttc
      361 ctggtggcgc gcgcgggctc ggccgagttc tcggtcgagg agcggaacct ccttcgcgcg
      421 atgggccgcg tcctcgagct cacgctgggg atgctcgagc gtcagcgact cctcgaacac
      481 ctgtcgagga tccagcggtc catctcgcac cgcgcgccgc tccgggaggt cctcgatgcg
      541 gtcacgaccg gggccagcga tctcctcggt gtcgaggtgg cgggtctgcg gctgatcgac
      601 cccggcgatc cggccttcgt gctcacggcc tccgccgccg gcgtcgatgc cgacgcgatg
      661 aaggcggtcc ttcggatgcc cctcggccag ggcgccggcg ggcgcgcgat cagcgaggac
      721 cggctcgtga tcatcgagga ctacgcctcg gctccaggca tggtcccgca cttcgcgagc
      781 cgcgcgctgc aggtggcgat ggccgccccg gtccacgaga acggcgtcgt cgtgggcagc
      841 ctcgtcgtgg cctcgtacaa gccgggacgg cgcttcagcg gggaggagca ggacgcgctg
      901 cttgccctcg ccgagcacgc gagcctcgcg ctcaccgacg ccaagatcgt cgaggagatg
      961 cgggaggcgc agcggtcgaa ggacctgttc ctcgcgatgg tctcgcacga gctcaagacg
     1021 ccgctcaccg tcatcatggg tgggctccgt accctcgagg tccacggcga ggacctcccc
     1081 gcggagctcc gccacgagat cctcgctgcc gggtggcacc gcggacgcga gctcgagcgc
     1141 ctgatcaacc ggctgctcca gggcgcacga gccgagctgg tgcctcgcgt cgaggacgtc
     1201 gacctgttcg agctggtcgc tgagggcgtg cgagggctcg agcacagccg tcgggtccgc
     1261 ttcgacgtcc cgacggacgt cgcgatccgc gtcgatcgcc cggccgtgct cgaggtcctc
     1321 gggatcctcc tggagaacgc ggtctcccac tcgggccccg gcaccgacgt ctggctcgac
     1381 gcgcgcgtcg agcgccgcga gctcgtcctc ggggtcgcga acgaagggca gctgccggag
     1441 ggcgtcgatc ccgagaccct cttccgaccg ttccagcgcg gagacgacac gcgttccccg
     1501 ggcgtgggcc tgggtctcta catcgcatcc cgcgtcgccg agtcgatcga cggaaaggtt
     1561 tccgtcgaga ccgacggcgg ccgcgtgcgg ttcgtccttc gggtcccggt gttcgaggtc
     1621 ggcgccggcc tgccggacgc tagacccgga cctcgagacg acatccgcgc acctgcaccg
     1681 atgcagcccg gccgttgagc atcgcttcgg tgaggccccg caggagccct tcctcgaggg
     1741 agcacgagcg cccgttctcg ccgggggcgc cgccgtggaa cagacagcgc ccggtcgtga
     1801 tgcgcatccc cccgttctcg ttgatcgcgc tcggacgcca gccccagcgt gccagctccc
     1861 ggctcagcag ctcagcccgc gccctcgggt cgcgcggggc tcgcccgttc ccacgcttgc
     1921 gcttcgaggt ccacgagcgg gcccagtcct cgccgaagcg cgtgagcttg cgccggtctc
     1981 ccggaagcgc gtcgaccgcc tcggccatca gcgagacgag cgggcgcatc gccgcgtccg
     2041 ggccaagctc gaacggctcg gggctgggcg cgtagacgcg tcgcggccgt cccgccccgc
     2101 cggggcgctc cagcgccgag acgatgagcc ccgcctgctc gagccgcgtg aggtgctgcc
     2161 ggacggcgtt cgggtggagg gagaaggctg cggcgagctc tgcgaccccc tcgggaagcg
     2221 gcgagagcca gatccgccgg tagatccgga agcgcgtctc ctcacccagc gccctcgccg
     2281 tctcgtagag acgatccagc ccctcggcgg cagccctccc catcggggcg atgatagacc
     2341 ggccgcctcg gacgcggggc caccggccga acggcccccg ccgggagggg gtcttgtcaa
     2401 accgggccgg cgggggtacc gttatgccct tcagatttct ccaggagata ttggagaaat
     2461 gcgaggaacc cgggcggggg ctccggcgtc caacggacga ggcacaggta tggcgatggc
     2521 agtcagtgat tgggagatgg gatggcagta ccgcgcatcc tgtcgcggcg aggatgcgga
     2581 gatgttcttc gcgcccaact acttcgagcg caaggacgag aaggacgcgc gcgaggttcg
     2641 cgcgaaggcg atctgcgctg actgtcccgt ccgcgtcgaa tgcctcgagt acgccctgcg
     2701 cagccgtgag ccccacggca tctggggcgg actgaacgag ctcgagcgca gggcgctcat
     2761 ccgtcagcgc gagcggcgcg ccggctgacg gcatggggtt cgacgatcga ctggatgagc
     2821 tcgggatcga gctgcccccg gcggccgccc ccgtcgcctc ctacgtcccg gtaaccacgg
     2881 cggcgggcct cgccttcgtc gcgggtcagg tcccgctcga gggcggcacc ccgatcgcga
     2941 ccggcagggt cggcgaagcc gtctccgtcg aggagggcaa cgcactggcg cgccgctgtg
     3001 cgctccaggc cctcgccgcg ctgcgccagg agctcggctc gctcgatcgc atccgtcgga
     3061 tcgcgaaggt caccgtctgg gtcgcctccc aggtcggctt cacggcacat ccgcaggtcg
     3121 cgaacggggc gagcgatgtg ctggtcgaga tcttcggcga cgtgggcaag cacgcccgcg
     3181 cggcggtcgg tgcgccgtct cttcccctcg gcgtgcccgt cgaggtcgag ctcgtcgtcg
     3241 agctgacgta gccgtttcag gcggcgcgcg agcgcgccga cgcgcggcgt cgcaggagcg
     3301 agagcacctc gatctcctcg cggcgcagct ccctgagcct cgaggcccac gccgctcgcg
     3361 agcgcttgag ctgttcgaag aggacgcctt cggtgtacgc ctcgatgacg tcgggatgca
     3421 cgtagcactt ccggcacacc gccggtgtgt tgcgcagctc cgccgcgacc tccttgatcg
     3481 ccgccgcgac ctcgcgcttc gcggcgcgct ccgagcgcgg cggctcgcgc cgacgaagga
     3541 cctgcgtagc cagcacggtc ccgccccagg tgcggaagtg cttcgcggtc acgtcgttcg
     3601 ccgcgatctc gcggaggtag gcgttcacgt cggcggaccc gaccgccacc ggcgtgccgt
     3661 cgtccgcgac gtactggaac aggtcgtagc cgggaaggtc gcggcacgcc cgcacgatcg
     3721 ccgccagacg cgggtcctcg acgctcaggc ggtgcttctt cccgcccttg ccgatgaact
     3781 cgaagagcac ctcttcgcgt cccacgtcga catggcggtc ccgcagggtc gtgagaccga
     3841 acgactggtt gtccctcgcg tactcggggt ttccgacgcg cgcgagcgtc gtgtcgagga
     3901 gccgcacgac ggtcgcgagg accttctcgc ggggaagccc gggaagcgcc aggtgacgtc
     3961 ggacccgcgc gcggatcgcg gagagcgcct cgccgaactc gatcaggctc gcgtacttcg
     4021 tctcgtcgcg gacccgccgc cactgctcgt ggtagcggta ctgcttccga ccgcggccat
     4081 cgcgcccggt ggcctggatg tgtccgcccg gtgtggcgca gatccacacg tcggtccacg
     4141 ccggcgggat cgcgagaccg cggatccgag cgagggtcgc accgtcgtcg atccgctcgc
     4201 cgtccggccc gaggtagacg aagccccgcc cggcgcggcg ccggcggatc ccgggggcgg
     4261 cgtcgctgac gtatcgcagc ccggcttcgc gcgcagcagc ttcggagatc tcggccgcgg
     4321 tcatggtggt gccagcgttc ccgcagcgga ggagtgggaa accgacgtgc cgatcagccg
     4381 gctcgtcgga cgtcgccctc gcgccgtgtc acgccacggg cgtccagcca gccgaggagg
     4441 gggagggcgt acttgcgtgt ggtcccgagg agctcgcgga agcggctcac cgtgagcccg
     4501 gagggtgtcg ccgctccgcg gcgggccagc tcctcggccc gggcggcgaa cgccggggtg
     4561 acgacgagct cctgggagac gcgcaccagg aggccggtcg cgcacgcggc gtccaccagg
     4621 tcgcggtcga acccgagccg ccccagctcg gcggcgtccg gcggcgtcgg ctcgcccgac
     4681 gcgacggcgg cgaccagctt ctgcgcctcc tcttcccggt ccccgagccc gacctggtgg
     4741 ccggggagtc gaaggagcgt cccctcgcgc acgacgtccc tccgggcttc caggtgcgcg
     4801 agcagcgctg gaaggagccc cgcctcgagc gccggccgga gcgtgccgag ggtcccggcc
     4861 agctcggccg cggcctcgtc catcgggaag ccggggacga gcgggttccc cgcgtgtcgc
     4921 tccgcgagga gccgcgtgag cgcgtcccgc gtctcctcca gcgcttcgtc ccggacccac
     4981 cacgccccga cccgctcggc cgcgcggatc gctccgggag aggatccggt gaggggcggg
     5041 agatccgtcg agcgcaccgc cgtacgctcg gcgacaagca cctccgcgag gtcgtgcggc
     5101 gccaggtgcc gccgggcctc gagcctggtc gcggccgcgc cggggcggtg gggcgggtcg
     5161 gcatccagca cctcaccgcc cgcgacggtc gcgcggcgac cggactcccg caggacgaac
     5221 gggtcgccga cggccgcgat cacgggctcg tcgagcttga gtcgggcgaa cccttccgtg
     5281 ccgggcgcca gctccctgcg gtcgtacagg tggaggcgga catcgcgctc ggcggcgccg
     5341 aggtagagct tgtaggcgcc tcgggcggtc agcgggtcgc ggagcgagcg gatcggccgc
     5401 aggcgaacct cgaggcgtga cgtcggccgc cactgctggg gtcgcgccac gacgtcgccg
     5461 cgggagacgg catcgcgtgg ggtcccggcg aggctcgcgg cgacgcgcga gaccgaccgc
     5521 gcctcctcca tcgtgcggcg gtgcgactga agtccccgga tgcggaccgt gcgcccggac
     5581 gggaagatcg ccacctcgtc tccgacggag agcgccccac cggcgagcgt ccccgtgacg
     5641 accgttcctg cgccggcgac ggagaaggag cggtccacgt ggagccgggg ccgatccgtc
     5701 tcgggctccg gtggcgccgt gcgcgccagc tcgtcgagcg cggagcgcag atcctcgatg
     5761 ccggcgcccg tcgcggccga gcagggaacg acgggggcgc cctccagcgc cgtgcccgcc
     5821 aggcgctctc cgatctcccg cagccgcgcc tccaccaggc ccccctcggc gaggtcggcc
     5881 ttcgtcagcg cgacgacggc agccccggct ccgagcaggt cgacgatccc gagatgctcc
     5941 tcggactgcg gcttccatcc ttcgtcggcc gcgaccacga agaggacgag ccgaacgggc
     6001 ccgacaccgg ccagcatgtt gcgcacgaac cgctcgtgcc ccggaacgtc gacgaagccg
     6061 atctcccggc ccgacgggag ggtcgtccag gcgaagccga ggtcgatcgt caggcctcgt
     6121 cgcttctcct cctcccaccg gtcggggtcc atcccggtga gcgccgcgac gagcgaggac
     6181 ttcccgtggt cgacgtgtcc cgcggtggcg acgacctgga gggcaccgct cacggcgcgg
     6241 gatcctcacg cagcgcgtag cggagcgccc ggatgaggag gtcgtcctcc ccgggcatga
     6301 cggtgcggag gtcgagcagg atcgcatcgt cggcgacgcg gcagaagacg ggcggcgtgc
     6361 cctgacggag ccgagccgcg agccgtcccg ggtcgcgcgc cgccaggcgg acggggtggg
     6421 cgatcccgac ccacctcgtc c
//
LOCUS       DATLGG010000237         4870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_93875, whole genome shotgun sequence.
ACCESSION   DATLGG010000237 DATLGG010000000
VERSION     DATLGG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93875"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VM638_06815"
     CDS             complement(<1..1412)
                     /locus_tag="VM638_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVM12167.1"
                     /translation="MSTFDPVGPAGFPALEERILRFWKEARIFEKTLELRKDAPEWVF
                     YEGPPTANGRPGIHHVESRTFKDVYPRYKTMTGHRVPRKAGWDCHGLPVELEVEKEIG
                     TRSKREIEEFGIAEFNRLCRESVTRYVEDWERLTERIGFWIDLSQAYWTMTPGYIESV
                     WWSLRELHRRGLLFEDDRITAYCPRCGTGLSDHEVSLGYTQVRDPSVEVRFPIVEAAD
                     PSLVGSSLLVWTTTPWTLISNTGAAVAPDETYVVAASGERSVILAEARIEVLGEGWEV
                     TRRLDGEALVGARYEPPFPNIDPGVAHRVVGASFVGMDEGTGIVHLAPAFGAEDLAVG
                     KAQGWPVFNPVDDQGRFTDRTSVGFVRGRFVKEADADIIEDLRARDLLMRAATIEHTY
                     PLCWRCDTPLLYYARPAWYIRTTARKERLLEVNERVAWYPDHIKHGRFGDWLENNVDW
                     SLSRERYWGTPLPVWRCREG"
     gene            1472..1864
                     /locus_tag="VM638_06820"
     CDS             1472..1864
                     /locus_tag="VM638_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HVM12168.1"
                     /translation="MPPTKSPFTKKQLEPIRKGLEEERRQLEKQAADLEQSSLGTPQS
                     EMTGEAGFDEEYADAGTATFEREKDLSLSNNIKDLMDQIDRALERMDAGVYGLCTRCG
                     NPIEKARIQALPYAELCIKDKQAEERSR"
     gene            1874..2377
                     /gene="lspA"
                     /locus_tag="VM638_06825"
     CDS             1874..2377
                     /gene="lspA"
                     /locus_tag="VM638_06825"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVM12169.1"
                     /translation="MSDPSRADAAARTGRLRILLASVAVAGYALDRVTKILAERHLEG
                     RPPIDLIDGVLRLRFATNPGGAFGLFGGATWLFLLASVAVVIVVIVAARKLPSRTSAV
                     GLGLILAGALGNLTDRAIRGPSFSGEVVDFIDLHVWPVFNVADSCIVVGAGLLLVAGF
                     GSSGEER"
     gene            2374..3264
                     /locus_tag="VM638_06830"
     CDS             2374..3264
                     /locus_tag="VM638_06830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVM12170.1"
                     /translation="MTRLEAEAPPGRLDAVVAALTGRSRADVQRAIVAGRVLVDGVAR
                     PKSHRLEGGERIEADLDGPADLEPDAEPLTIRHEDEHLAVVVKPAGVVTHPTRTRREG
                     TFVNRLLGMGMPLSSIDPERPGIVHRLDAGTSGLLLVAKTDEAHRALVEAMKERRVRR
                     EYLALVRGSVRHDAFGIDAPLGRRGARVVLRAAGGVDAATDIEIRERLPAATLLVARP
                     RTGRTHQIRVHLASVGHPILGDRAYGGGGDEATRLRLDRPFLHAWRLALDHPLTGGRL
                     EVEEPLPRDLDAALRRARGD"
     gene            3298..4857
                     /locus_tag="VM638_06835"
     CDS             3298..4857
                     /locus_tag="VM638_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12171.1"
                     /translation="MAARVSERPLPLPVLPLALSLSLYTAATIIGLLPPVNLLIPGVR
                     IAVETAGAIISVLTAALAFVQYEADRLRSWLLIAIAFVTSAANQFLFGVIAAPPGPLI
                     VYLRTTGRLAVALLLLSAALQPGARLLARRPLAAGFARAAATLAVVAVAQVGLWLARE
                     GLPDLRPAGSIPSLRGGGVAPLLAIPSVISAALILTSALLFLRPQALLRATWLVPALL
                     LGGFSHLHYALSPTLFTDQISNGDLLRLGFLAVLLFGVVGAIRQGRLAERRRSLQMAR
                     AYVDERQHARELEDLDRAKQELLAIVSHELMHPVAAIRGFALTLERRWDELEEATRLE
                     IVGHIAEQSERLRRLAEEGGAALSTNLERLDVVPEPQDVRDLLEEAARMAPELAGRLE
                     IRVRDGAVETFALADRARVQQVFGNLLSNAAKFSPADSPVELGADAVDREIRFWVRDQ
                     GPGVDPADRAKLFEPFARLAASDGKPGTGLGLYIARRIVEAHGGRLWVESRAWEGATF
                     AFTVLSAEAAV"
BASE COUNT          666 a         1753 c         1723 g          728 t
ORIGIN      
        1 tgaccctcgc ggcagcgcca gaccggcaac ggcgtccccc agtagcgctc gcgggagagc
       61 gaccagtcga cgttgttctc gagccagtcg ccgaaccggc cgtgcttgat gtggtccggg
      121 taccaggcga cccgctcgtt cacctcgagg agacgctcct tgcgggcggt cgtgcggatg
      181 taccaggccg ggcgcgcgta gtaaaggagc ggtgtgtcgc agcgccagca cagcgggtac
      241 gtgtgctcga tcgtggcggc ccgcatcagc aggtcccgcg cgcgcaggtc ctcgatgatg
      301 tcggcgtccg cctccttcac gaagcggccc cggacgaacc cgacggaggt gcggtccgtg
      361 aagcgtccct ggtcgtcgac ggggttgaac accggccacc cctgcgcctt ccccaccgcg
      421 aggtcctccg ccccgaacgc cggggcgagg tggacgatcc cggtcccttc gtccatcccg
      481 acgaacgagg ccccgacgac gcgatgcgcc accccggggt cgatgttcgg gaagggcggc
      541 tcgtagcgcg cgccgacgag ggcctcgccg tccaggcgtc gcgtgacctc ccagccttcg
      601 cccagcacct cgatccgcgc ctccgcgagg atcaccgacc gctcaccgga ggccgccacc
      661 acgtacgtct cgtcgggggc gacggccgcg ccggtgttcg agatgagggt ccagggcgtc
      721 gtcgtccaca cgagcagcga ggagccgacc agggaggggt cggctgcctc gacgatgggg
      781 aagcggacct cgacgctcgg gtcgcggacc tgcgtgtacc cgagcgagac ctcgtggtcg
      841 gagagccccg tgccgcaccg tgggcagtac gccgtgatcc ggtcgtcctc gaacagcagc
      901 ccgcgacggt ggagctcccg cagcgaccac cagacgctct cgatgtagcc gggcgtcatc
      961 gtccaatagg cctgcgagag gtcgatccag aacccgatcc gctcggtcag ccgctcccag
     1021 tcctcgacgt aacgggtcac cgactcccgg cagagccggt tgaactccgc gatcccgaac
     1081 tcctcgatct cgcgcttcga gcgcgttccg atctccttct cgacctcgag ctcgaccggc
     1141 aggccgtggc aatcccaccc cgccttgcgg gggacccggt ggcccgtcat cgtcttgtag
     1201 cgcgggtaga cgtccttgaa cgtcctcgac tcgacgtggt ggatccccgg gcggccgttc
     1261 gccgtcgggg gcccctcgta gaagacccac tccggcgcat ccttccgcag ctcgagggtc
     1321 ttctcgaaga tccgcgcctc cttccagaag cgcaggatgc gctcctcgag ggccgggaag
     1381 ccggccgggc cgacggggtc gaacgtgctc actcgctcct ccggtgggtc cgttgcgacg
     1441 gggacggggg ccatgctacc gtccgccaac catgcccccc acgaagagcc ccttcacgaa
     1501 gaagcagctc gagccgatcc gcaagggcct cgaggaggag cggcgccagc tcgagaagca
     1561 agccgccgac ctcgagcaga gcagcctcgg cacgccccag tcggagatga cgggcgaagc
     1621 cgggttcgat gaggagtacg ccgacgcggg caccgcgacg ttcgagcgcg agaaggacct
     1681 ctcgctctcg aacaacatca aggacctgat ggaccagatc gaccgggccc tcgagcggat
     1741 ggacgcgggg gtctacgggc tctgcacgag gtgcgggaac ccgatcgaga aggcccggat
     1801 ccaggcgctg ccgtacgccg agctctgcat caaggacaag caggccgagg aacgttcccg
     1861 ctagcccggc gcgatgtccg acccgagccg ggccgatgcc gccgcacgca ccggacgcct
     1921 ccgcatcctc ctcgcgagcg tggccgtcgc cggatacgcg ctcgaccggg tgacgaagat
     1981 cctcgcggag cgccacctgg aagggcggcc gccgatcgac ctgatcgacg gcgtcctgcg
     2041 tctgcgcttc gcgacgaacc ccgggggggc cttcggcctg ttcggcggcg ccacctggct
     2101 gttcctcctc gcgagcgtcg ccgtcgtgat cgtcgtgatc gtcgcggctc ggaagctccc
     2161 gtcgcgcacg agcgccgtcg ggctcggcct gatcctggcg ggcgccctgg ggaacctgac
     2221 cgaccgggcg atccgtgggc cgagcttcag cggggaggtc gtggacttca tcgaccttca
     2281 cgtctggccc gtcttcaacg tcgccgacag ctgcatcgtc gtgggtgccg ggctgctgct
     2341 cgtcgccggc ttcgggtcgt cgggcgagga gcggtgacgc gtctcgaggc ggaggcgccg
     2401 ccgggcaggc tcgacgcggt cgtcgccgcc ctcaccgggc ggtcccgggc cgacgtccag
     2461 cgggcgatcg tcgccgggcg cgtcctcgtc gacggcgtcg ctcggccgaa gagccaccgc
     2521 ctcgaggggg gcgagcgcat cgaggcggac ctcgacggtc ccgctgatct cgaacccgac
     2581 gcggagccgc tgacgatccg tcacgaggac gagcacctcg cggtcgtcgt gaagccggcc
     2641 ggggtcgtga cgcacccgac ccggacacgc cgtgaaggga cgttcgtgaa ccggctgctc
     2701 gggatgggga tgccgttgtc ctcgatcgac ccggagcgcc cgggcatcgt ccaccgtctc
     2761 gatgccggca cctccgggct gttgctggtc gccaagaccg atgaggcgca ccgtgcgctc
     2821 gtcgaggcga tgaaggagcg gcgggtccgc cgggaatacc tggcgctcgt ccgcgggagc
     2881 gtccgccacg acgccttcgg catcgacgcg ccactcggcc ggcggggggc gcgggtggtc
     2941 ctgcgcgcgg ccggcggtgt cgacgcggcg accgacatcg agatccgcga gcgcctcccc
     3001 gcggccaccc tcctcgtcgc gcgtccccgc acggggcgaa cgcaccagat ccgggtccat
     3061 ctcgcctccg tcgggcaccc gatcctcggc gaccgggcct acgggggcgg gggagacgag
     3121 gcgacgcgtc tccgcctcga ccggcccttc ctccacgcgt ggcgcctcgc gctcgaccac
     3181 cccctcaccg ggggccgcct cgaggtcgag gagccgctgc cgcgggatct cgatgccgcc
     3241 ctccgacgcg cacgcggcga ctagcggcgc ggctcagtgc cgcactatga tgcgccaatg
     3301 gcggcccgcg tttccgagcg accgctcccg ctgcccgtgc ttccgctcgc gctgtcgctc
     3361 tcgctgtata cggcagcaac tatcattggg ctccttccgc cggtcaacct gctgatcccg
     3421 ggcgtacgga tcgccgtcga aacagccggt gcgatcatct cggtcctcac cgcggcgctc
     3481 gccttcgtgc agtacgaggc ggaccgcctc cgctcctggc tgctgatcgc gatcgccttc
     3541 gtgacgagtg cggccaacca gttcctcttc ggcgtgatcg ccgcaccgcc gggccccctc
     3601 atcgtctacc tgcgaacgac ggggcggctc gcggtcgcgc tgctcctgct ctcggccgcc
     3661 ctgcagcccg gggcccgcct cctcgcgcgg cgccctctgg cggcagggtt cgcacgagcc
     3721 gccgcgaccc tcgcggtggt cgcggtcgcc caggtcgggc tctggctcgc tcgcgaaggg
     3781 ttgccggacc tgcggcccgc cgggagcatc ccgtcgttga gggggggcgg cgtcgcgccg
     3841 ctcctggcga taccgagcgt catctcggct gcgctgatcc tcacgtctgc cttgctcttc
     3901 ctccgccctc aggcgctgct gcgcgccacg tggctggttc ccgcgctgct gctcggaggg
     3961 ttcagccatc tgcactacgc cctctccccg acgcttttca ccgaccagat ctcgaacggc
     4021 gacctcttgc gtctcgggtt ccttgcggtc ctgctgttcg gggtggtcgg tgcgatacgc
     4081 cagggccgcc tggccgagcg gcgccgctcc ctccagatgg cgcgcgcgta cgtcgacgag
     4141 cgacagcacg ctcgcgagct ggaggatctc gaccgtgcga agcaggagct cctggcgatc
     4201 gtctcgcatg agctgatgca tccggtggcg gcgatccgcg gattcgcgct cacgctggag
     4261 cggcgatggg acgagctcga ggaggcgacg cgcctggaga tcgtcggaca catcgcggag
     4321 cagagcgagc gcctccgtcg gctggcggag gagggcggcg cagctctcag cacgaacctc
     4381 gagcgcctcg acgtcgtccc ggagccacag gacgtgcggg atctcctcga agaggcggcg
     4441 cgcatggccc cggagctggc gggacggctg gagatccggg tccgggacgg agcggtggag
     4501 acgttcgcgc tggcggaccg agcgagggtt cagcaggtgt tcgggaacct cctgtcgaac
     4561 gccgccaagt tcagccctgc ggactcgccc gttgagctcg gtgcggatgc cgtcgaccga
     4621 gagatccggt tctgggtgcg ggaccaggga ccgggcgttg acccggcaga cagggcgaag
     4681 ctgttcgagc cgttcgcgcg gctggctgct tccgacggca agccgggaac cgggctcggg
     4741 ctgtacatcg cccgccggat cgtggaggcc cacggaggtc ggctgtgggt cgaatcgcga
     4801 gcgtgggagg gggccacgtt cgccttcacc gttctgagcg cggaggcggc ggtttgagcg
     4861 agccgtggtc
//
LOCUS       DATLGG010000238         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_213789, whole genome shotgun sequence.
ACCESSION   DATLGG010000238 DATLGG010000000
VERSION     DATLGG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213789"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VM638_06840"
     CDS             complement(<1..468)
                     /locus_tag="VM638_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HVM12172.1"
                     /translation="MPVRERIERAAGAAEAARLDALIVAPGADLRYLLDHDPPPLERL
                     TCLVIRPQTEPVLLVPELERRLAEDAGVGGVAALVGWRDGTDPYALVRRMLGRARRVA
                     CSDRMWASHLLRLQQALGQARFASASIVLGPLRAVKEPGEVDQLARAARYADEA"
     gene            complement(487..1404)
                     /locus_tag="VM638_06845"
     CDS             complement(487..1404)
                     /locus_tag="VM638_06845"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVM12173.1"
                     /translation="MSSPFGRLVMIAEPAAGRGRVGEELPEIERQLQAGKLRYRFVEA
                     AAPGEATRLAREALAEGDRFLVAVGDDDTLHDVVNGMVEGGRPVNENAVLGMVAAGSG
                     SDFVRTFGLPGDAIRAVRHLGGHRTYPIDAVAVRCMTPEGGDAERVFVNVAQVGFGAG
                     VVQRSGGVRSSGLRHLLGFWRTLATQKPFEAIVRADRKTLERRATNVIVANAQYRGGG
                     MRISPRSYPGDGLLDVQVWIGPRSEAFTAIPKLYRGEHVPHPNIKELRGREISVTTDP
                     PVAVEVDGRVIGHTPATFSILHEALRLKT"
     gene            complement(1406..>2151)
                     /locus_tag="VM638_06850"
     CDS             complement(1406..>2151)
                     /locus_tag="VM638_06850"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HVM12174.1"
                     /translation="PPIWRRRRDARLAHRSCVSRSGHPVTDCPDLETHERWASRASRL
                     RRQIGGVERRIRSRTDSLARQFDRVLGVLRELGYVEDFKLTERGERLTRIFGEGDIVV
                     AEALAEGHLDGIGPPTFAALVSTFVYESRERQPRAPVLSGDLRARFRRLQELWQRVRR
                     VEERHGVELTRELDAGFAAVALHWAEGEPLEEILAEAGLAAGDFVRTCKQLLDLLRQI
                     AEVADGPAAEVARAARDAVNRGVVAYTGI"
BASE COUNT          298 a          824 c          753 g          276 t
ORIGIN      
        1 ggcctcgtcg gcgtaccgcg cggcccgagc gagctgatcc acctcgcccg gctccttcac
       61 ggcgcgcagc ggtccgagca cgatcgacgc ggacgcgaac cgtgcctgcc cgagcgcctg
      121 ctggagacgc agcaggtgcg aggcccacat ccggtcggag cacgccacgc gccgggcccg
      181 cccgagcatc cgtcgcacga gggcgtacgg gtcggtcccg tcgcgccatc ccacgagcgc
      241 cgcgacgcct cccacgcccg catcctcggc gaggcgcctc tccagctcgg gcacgaggag
      301 caccggttcc gtctgggggc ggatcaccag gcacgtgagg cgctcgagcg gcggcggatc
      361 gtggtcgagc aggtagcgca ggtccgcgcc cggcgcgacg atcagcgcgt cgagccgcgc
      421 cgcctccgcc gcgcccgcgg cacgctcgat ccgctcccgc accggcatcc gcccatgata
      481 ggggcctcag gtcttgagcc ggagcgcttc gtgcaggatc gagaaggtcg ccggcgtgtg
      541 accgatcacg cgtccgtcca cctcgaccgc cacgggcggg tcggtcgtga ccgagatctc
      601 ccggccgcgc agctccttga tgttcggatg gggaacgtgc tcgccgcggt agagcttcgg
      661 gatcgcggtg aacgcctcgg agcgtgggcc gatccagacc tgcacgtcga gcagaccgtc
      721 gccgggatag gaacgcgggc tgatccgcat cccgcccccg cggtactgcg cgttcgcgac
      781 gatgacgttg gtggcgcgcc gctcgagggt cttacggtcc gcccggacga tggcctcgaa
      841 cggcttctgg gtcgcgagcg tgcgccagaa gcccaagagg tggcgcaaac ccgagctccg
      901 gaccccgccg gatcgctgga cgacgccggc gccgaagccc acctgagcga cgttcacgaa
      961 cacgcgctcg gcgtcgcctc cctccggggt catgcagcgg accgccaccg cgtcgatcgg
     1021 gtacgtccgg tgaccgccga ggtggcgcac cgcgcggatc gcgtcgccgg gcaggccgaa
     1081 ggtccggacg aagtcggagc ccgagcccgc ggcgaccatc cccagcacgg cgttctcgtt
     1141 cacgggccgc cctccctcca ccatgccgtt caccacgtcg tgcagggtgt cgtcgtcgcc
     1201 gacagccacg aggaaccggt ccccttcggc gagcgcctcg cgcgcgagcc gcgtcgcctc
     1261 accgggcgcg gcggcctcga cgaagcggta ccggagcttc cccgcttgga gctgtcgctc
     1321 gatctccgga agctcctccc ccacccggcc gcgtccggcg gccggctccg cgatcatgac
     1381 gagacgcccg aagggactgc tcatctcaga tccccgtgta ggcgacgacg ccgcggttca
     1441 cggcgtccct cgcggcccgg gcgacctcgg ccgcgggtcc gtccgcgacc tccgcgatct
     1501 ggcgcaggag gtcgagcagc tgcttgcagg tgcgcacgaa gtcgcccgcc gcgagccccg
     1561 cctcggcgag gatctcctcg agcggctccc cctcggccca gtgcagcgcg acggcagcga
     1621 agccggcatc gagctcacgt gtcagctcga cgccgtgacg ctcctcgacc cgacggaccc
     1681 gctgccacag ctcctgcagg cggcggaacc gggcgcggag atccccgctc aacacggggg
     1741 cacggggctg gcgttcccgc gactcgtaga cgaacgtgct gacgagcgcg gcgaacgtcg
     1801 gggggccgat cccatccagg tgaccttccg cgagggcctc cgccacgacg atgtctccct
     1861 ccccgaagat ccgcgtcagc cgctcccctc gctcggtgag cttgaagtcc tcgacgtagc
     1921 cgagctcccg gaggaccccg aggacccggt cgaactgccg ggccagcgag tcggtgcggg
     1981 agcggatccg ccgctcgacc cccccgatct ggcgccgcag acgcgacgct cggctcgccc
     2041 accgctcgtg cgtctcgagg tcggggcagt cggtgaccgg atgccccgac ctcgagacgc
     2101 acgagcggtg ggcgagccga gcgtcgcgtc tgcggcgcca gatcgggggg g
//
LOCUS       DATLGG010000239         5015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_94015, whole genome shotgun sequence.
ACCESSION   DATLGG010000239 DATLGG010000000
VERSION     DATLGG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94015"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VM638_06855"
     CDS             <1..245
                     /locus_tag="VM638_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12175.1"
                     /translation="LVVEDDGPGIPREDVPRIFERFFKADPARRREEGRGSGLGLAIA
                     RENAHLHGGEIEVASEEGHGARFTVRLPLTPAEDTR"
     gene            245..799
                     /locus_tag="VM638_06860"
     CDS             245..799
                     /locus_tag="VM638_06860"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HVM12176.1"
                     /translation="MRPRGALVILLLTTAACARQGVTLIPESELDEIYTSPSPSPRPG
                     LPRTGVVYLVEDDRLAATRRGLDPAPTLAEALLEALLREGDRDESRSAIPPETRVLDV
                     GVVGGVATVDLSQGFEQGASGSDLALRVAQIVYTLTEDPEIRAVLIAIEGEVAPVIAG
                     NQRDVLDRPVTRDDYRALGPPDAD"
     gene            880..1152
                     /locus_tag="VM638_06865"
     CDS             880..1152
                     /locus_tag="VM638_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12177.1"
                     /translation="MSAIFRDHPTAAPAAVRDASRPEPTGRRARTASLVDPPPPGDDL
                     AIVEVSLPSGDPLSTQARVEEGLWDHVIWGGESYPDDLLADTDLLR"
     gene            complement(1149..1847)
                     /locus_tag="VM638_06870"
     CDS             complement(1149..1847)
                     /locus_tag="VM638_06870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVM12178.1"
                     /translation="MAERGQEEIRPDDPGSIRRMFRELAPDYDRLNSVLTFGLVHRWR
                     RALVRTLGAGPGGRYVDVCAGTGMSTRAVRRAAGEEAVVVGVDFTPEMLERSDGLRVL
                     GDALRLPFPDGTFDGAISSFALRDIADPVASLGEMARVLRPGGRLGILEIGRPRSALP
                     RAGFDVWFRGAVPRVAGAFGHRRSHDFLVRSVRYLPPPEELCRELERLGLVEVGWRPL
                     TLGAASLFVARRGT"
     gene            1907..3358
                     /locus_tag="VM638_06875"
     CDS             1907..3358
                     /locus_tag="VM638_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12179.1"
                     /translation="MRRPVSIASILLLLATPAGGQDTSASDPRYLAYIEAEENDGTGG
                     ALVVVRSDGKEEPRTLLERGVLAADIAPGVPDRVDDPAGGETLPESRLLKIDIETGER
                     EDVLPDETAHYLGVAASPRGDIAFYRTVARAVEPPSHLAPAIPELRQAAVPVLAPPEE
                     PPDTGNVAADGHEDGYALRYTNDPTGGAAHAEQRVVFVEATVAEELPVAEGEGESVTV
                     RGTQGVFYCGAATCFVSWRENETTYTVGEFGDQGEAIAFAESLQPMEDLAGIGWQSEE
                     PFPTPELIVLREDGERVTLESVEGFCECGFPPVSWRPSARRLLVAAMAEGYTTLQEYR
                     ANGRGRPRTLAEGSIFEGPGGIFDAAYAGDRVLTLVAGDSGPPGDLRALGGGVLREDV
                     RAFDTHGSLLAYVTGGGAVRLLDLTTDEEVRLARGAIDVSIAPDTIEEDAPPPAPNDE
                     GPPLVLLVLILLALASLGLGTALYVAARRRRRR"
     gene            complement(3469..4587)
                     /locus_tag="VM638_06880"
     CDS             complement(3469..4587)
                     /locus_tag="VM638_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12180.1"
                     /translation="MAGGEPAAASSMLGIAWSDVAWAVGVAATVWLLGWLTIVWAGRE
                     RREPEALESDLGPERTEHAGPTPVSGVLRWLMAETEADAAALFTVAPGRGERVVVEPR
                     GLDAASIAALARAGRDALLRPDEEAGSGIVTTRWLGSGGSKALVLSGVPRSTAEEPMR
                     FARFAIESGSTTPGALAERAVEERVRAIPGVAWAELDPQDPTRVRVLSADGIDRSILE
                     QEIGRVVAGTGARVHWVDEPDPREAGAMPRARLVAASIATNGQAHAQVDLDWLGQPLR
                     GRGHAGATHAGRYRAAADAMADALGPLLAGEVEVEGLYAHTHGEGELVVVVVTLDGER
                     LVGAVEARRADQDATAARAVLDAVNRRLAVMAGGSGRI"
     gene            complement(4685..>5015)
                     /locus_tag="VM638_06885"
     CDS             complement(4685..>5015)
                     /locus_tag="VM638_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12181.1"
                     /translation="LIIERLIEASAENAELRVAVERARTEVEAARREAMEVTRTSSAD
                     RRRAQNLEDEVAALRKRLEMTESNLRTVVQAAKTRRSSPLEDSDAKAILDILSTTGAS
                     EEGEPGA"
BASE COUNT          671 a         1901 c         1804 g          639 t
ORIGIN      
        1 gcctcgtggt cgaggacgac gggcccggca tcccgcgcga ggacgtcccc cggatcttcg
       61 agcggttctt caaggcggac ccggcgcgtc ggcgggagga gggccgcgga tccgggctcg
      121 ggctggcgat cgctcgggag aacgcgcacc tgcacggcgg cgagatcgag gtggcgagcg
      181 aggaggggca cggcgctcgg ttcacggtcc gcctcccgct gaccccggcg gaggacacgc
      241 gttgatgcgg ccccgtggcg ctctcgtgat cctgctgctc accacggccg cctgcgctcg
      301 ccagggggtc accctgatcc ccgagtccga gctcgacgag atctacacct cgcccagccc
      361 cagcccgagg ccgggccttc cgcgcaccgg ggtcgtctac ctcgtcgagg acgaccgcct
      421 cgccgcgacg cgccgcgggc tggaccccgc cccgacgctc gccgaagcct tgctcgaggc
      481 gctgctgcgg gagggcgatc gggacgagtc acgctcggcg atcccacccg agacgcgggt
      541 cctcgacgtc ggcgtcgtgg gcggcgtcgc cacggtggat ctgtcgcagg gcttcgagca
      601 gggggcctcg ggcagcgacc tcgccctccg ggtcgcgcag atcgtctaca cgctcaccga
      661 ggaccccgag atccgcgcgg tgctgatcgc gatcgaaggc gaggtcgcgc cggtgatcgc
      721 cggcaaccag cgcgacgtgc tcgaccggcc ggtcacgcgg gacgactacc gcgccctcgg
      781 cccgccggac gccgactagc gccgacactc gtcacggaac cgtcacacgc ccgacgcgtg
      841 aacgaaaccc gtcgcgggca tgctctggac gctgtggcga tgagcgcgat cttccgcgac
      901 cacccgacgg ccgcccccgc ggccgtgcgc gacgcatctc gcccagagcc aacgggtcga
      961 agggctcgga cggcatccct ggtggaccct cctcctccgg gcgatgatct cgccatcgtc
     1021 gaggtcagcc tcccatcggg ggatcccctc tctacccagg ctcgggtgga ggagggtctc
     1081 tgggaccacg tgatctgggg cggcgagagc taccccgacg acctcctggc cgacaccgac
     1141 ctcctccgct aggtcccgcg gcgcgcgacg aacaggctcg cggcaccgag cgtcagcggc
     1201 cgccatccga cctcgacgag gccgaggcgc tcgagctcgc ggcacagctc ctcgggtggt
     1261 gggaggtatc gcaccgaccg aacgaggaag tcgtgggagc gtcggtggcc gaaggcaccg
     1321 gcgacgcgcg ggaccgcacc gcggaaccag acgtcgaagc cggcgcgcgg cagcgccgag
     1381 cgcggacggc cgatctcgag gatcccgagc cggccgccgg gtcggagcac gcgcgccatc
     1441 tccccgagcg aggcgaccgg atcggcgatg tcgcggaggg cgaacgagct gatcgcgccg
     1501 tcgaaggtcc cgtcggggaa cgggagccgc agggcgtcgc cgagcacccg cagcccgtcc
     1561 gaccgctcga gcatctcggg ggtgaagtcc acccccacga cgacggcctc ctcgcctgcc
     1621 gcccgccgca ccgctcgcgt tgacatcccg gtgcccgcgc agacatcgac gtatcgtccg
     1681 ccggggcccg caccgagcgt gcgaacgagc gcccgccgcc agcggtggac gagcccgaac
     1741 gtgaggacgc tgttgagccg gtcgtagtcg ggcgcaagct cgcggaacat ccgccggatc
     1801 gaccccgggt cgtctggtcg gatctcctcc tgcccccgct cagccacgag ccgcaccgta
     1861 gcagcggttc cacgatcgac gcgatggccg gtggtaccct ccggccgtgc ggcgaccggt
     1921 ctccatcgca tcgatcctcc tcctgctggc cacgcccgcc ggcgggcagg acacctcggc
     1981 ctcagatccg aggtatctcg cctacatcga ggccgaggag aacgacggga ccggcggcgc
     2041 cctcgtcgtc gtccgcagcg acggaaagga ggagccgcgg acgctcctcg agcgaggcgt
     2101 gctcgccgcg gacatcgccc ccggcgtccc ggatcgcgta gatgatcccg ccgggggcga
     2161 gaccctcccc gagagccgcc tcttgaagat cgacatcgag accggcgagc gggaagacgt
     2221 cctgccggac gagaccgcgc actacctcgg cgtcgccgcc tctccccgcg gcgacatcgc
     2281 gttctaccgg acggtcgcgc gagccgtgga gccgccctcg cacctcgcac ccgcgatccc
     2341 ggagctgcgc caagccgcgg tgccggttct cgccccgccg gaggaaccgc cggacaccgg
     2401 gaacgtcgcc gccgatgggc acgaggacgg ctacgccctg cggtacacga acgatccgac
     2461 gggcggagcg gcccacgcgg agcagcgcgt cgtcttcgtc gaggcaacgg tcgccgagga
     2521 gctccccgtc gccgaggggg agggcgagtc cgtgacggtc cgcggaacgc agggggtctt
     2581 ctactgcggc gcggccacct gcttcgtctc gtggcgcgag aacgagacga cgtacacggt
     2641 cggcgagttc ggcgaccagg gagaggccat cgcgttcgcc gagtcgctgc agccgatgga
     2701 ggacctcgcc ggcatcggct ggcagtcgga ggagccgttc ccgacgccgg agctgatcgt
     2761 gctccgcgag gacggcgagc gcgtgaccct cgagtcggtc gaagggttct gcgagtgcgg
     2821 gttcccgccg gtgtcgtggc ggccgagcgc ccggcggctg ctcgtcgcgg cgatggcgga
     2881 ggggtacacg accctgcagg agtaccgggc gaacggccgc ggccgcccgc ggaccctcgc
     2941 ggagggctcg atcttcgagg ggccgggagg gatcttcgac gcggcctatg ccggggaccg
     3001 cgtgctcacg ctcgtcgcgg gtgactcggg accgccggga gacctgcgag ccctcggcgg
     3061 gggggtcctg cgcgaagacg tccgggcgtt cgacacccac ggctccctcc tcgcctacgt
     3121 cacggggggt ggcgccgtgc ggctcctcga cctcacgacc gacgaggagg tgcggctggc
     3181 gcgcggggcg atcgacgtga gcatcgctcc cgacacgatc gaggaggacg cgccgccgcc
     3241 cgcgccgaac gacgaggggc cgccgctggt cctgctcgtc ctgatcctgc tcgccctcgc
     3301 gtctctgggg ctcggcaccg ccctgtacgt cgcggcccgc cggcgtcgcc gccgctgatc
     3361 ggcggagcag gagccgcggg ggcggcgtcg aatacctcgg gtaccaccgc gagtccccct
     3421 cagacccccg ccgccgaacc accccagcgg gggtcttccc tttcgggctc agatccggcc
     3481 gctgccgccg gccatgaccg cgagtcggcg gttcaccgcg tcgagcaccg cacgcgccgc
     3541 ggtggcgtcc tggtcggcgc gacgtgcctc gacggccccg acgagccgct ccccgtcgag
     3601 cgtcaccacg acgaccacga gctcgccctc gccgtgcgtg tgcgcgtaca gcccctcgac
     3661 ctcgacctct cccgccagca gcggcccgag ggcgtcggcc atcgcgtccg ccgcagcgcg
     3721 gtagcgaccc gcatgggtcg cacccgcgtg gccgcggcct cgcagcggct ggccgagcca
     3781 gtcgaggtcc acctgggcgt gcgcctggcc gttcgtcgcg atggaggccg cgacgagccg
     3841 cgcccgcggc atcgcgccgg cctcgcgggg atccggctcg tcgacccagt gcacgcgtgc
     3901 gccggtgccg gcgaccaccc gcccgatctc ctgctcgagg atcgagcgat cgatcccatc
     3961 ggctgagagc acgcggaccc gcgtgggatc ctgcgggtcc agctcggccc aggccacgcc
     4021 ggggatggcc cgcacgcgct cctccacggc gcgctccgcc agcgcgccgg gggtcgtgct
     4081 gccggattcg atcgcgaacc gcgcgaagcg catcggctcc tcggccgtcg acctcgggac
     4141 gccgctgagc accagcgcct tggaaccgcc gctcccgagc cagcgcgtcg tgacgatgcc
     4201 ggagcccgcc tcctcgtcgg ggcgcagcag ggcgtcgcgt cccgcgcggg cgagcgccgc
     4261 gatggaggcg gcgtcgaggc cccgcggctc caccacgacc cgctcacccc gtccgggggc
     4321 gacggtgaag agcgcagccg cgtcggcctc ggtctcggcc atcagccacc gcaggacgcc
     4381 ggagacgggg gtgggcccgg catgctcggt gcgctcgggc ccgaggtcgc tctcgagggc
     4441 ttcgggttcc cggcgctcac ggccggccca gacgatcgtc agccagccca acagccagac
     4501 tgtcgctgcg acaccgacag cccaggcgac atcgctccaa gcgatcccca gcatgctcga
     4561 agccgcggcg ggctcgcctc cggccacgcg tacgtccctc cctccgaccc tttgctgtag
     4621 ggcgatccag cgggcttgcg ggggccattc taccggcccg ggcatcccct tcccaacgga
     4681 ggggtcaggc ccctggctcg ccctcctcgg aggctcccgt cgtcgacagg atgtcgagga
     4741 tggccttcgc gtcgctgtcc tcgagggggg atgagcgtcg ggtcttcgcg gcctgcacga
     4801 cggtccgcag gttggactcg gtcatctcga gccgcttgcg gagggcggcg acctcgtcct
     4861 cgaggttctg cgcccgacgg cggtcggccg aggacgtgcg tgtgacctcc atcgcctcgc
     4921 gacgcgctgc ctcgacctcg gtccgcgcgc gttcgacggc gacgcgcagc tccgcgttct
     4981 cggccgaggc ctcgatcagc cgctcgatga tgagg
//
LOCUS       DATLGG010000240         7448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_94018, whole genome shotgun sequence.
ACCESSION   DATLGG010000240 DATLGG010000000
VERSION     DATLGG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94018"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="rplU"
                     /locus_tag="VM638_06890"
     CDS             complement(<1..380)
                     /gene="rplU"
                     /locus_tag="VM638_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HVM12182.1"
                     /translation="MYAVVRVGGKQQRVEPGDVIEVEYMKKLDPGSTVELDTLFVMED
                     GTVHRPKGGTVKVVGRLVGEKKGDKIKVFKYRNKSGYARTQGHRQLMTELEIEEIVVG
                     DKVLRKPEPAQEAATGSGEDAKKTT"
     gene            complement(389..2008)
                     /locus_tag="VM638_06895"
     CDS             complement(389..2008)
                     /locus_tag="VM638_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HVM12183.1"
                     /translation="MTSDPPGDASPGAASDAADADDRADPTEASDAPEGGTSSSTKRR
                     RRRRRRKKPSDSGDGEAAADPSSEAKPDTKPASKPADGRRSRAKPKQKEDEAPPATRT
                     ARRRRGVVTRRRCEPEIRPERRLDKIMLITENGDRDQIAVAEGRELVEHYVTRAGARS
                     MVGNVYLGRVQNVLPGMEAAFIDLGRGRNAVLYAGEVSHDEDVEEQPRRIEQVLKPGQ
                     PIVVQVTKDPMGGKGARLTGQISLAGRYLVYVPGGSASGISRRLPEKERNRLRGIMKE
                     IRPSGAGVIIRTAAEGATPEGLEEDLERLKKTWESIRRKSRRGKPPKVLYEEPELTIR
                     VLRDVFSAVEFREVVTDSESVYERVTAYLKEVAPDILDRVRLHQGPLPVFEEYHVHEQ
                     IHKALERKVWLPSGGYLIIDRTEAMTVVDVNTGKAVGKTNLEETVVQTNLEAAREIAR
                     QLRLRDIGGIIVIDFIDMLLQANRNKVIRALKEEMAQDKTRSQVFDITPLGLLEVTRK
                     RVSAGLVESFSQTCPTCEGRGILVSLDTEGN"
     gene            complement(2612..3778)
                     /gene="rodA"
                     /locus_tag="VM638_06900"
     CDS             complement(2612..3778)
                     /gene="rodA"
                     /locus_tag="VM638_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HVM12184.1"
                     /translation="MDVSAGRSLDVGAGRLLASERSPLRHLDPMLVVTTLALTIYGLM
                     LIYSATHQSIRAFDRTADPGLFMKKQFAFMLLGLIAMILVATFDYRLAKVYAPITYGA
                     VVILLLLVMTPLGNRTLGAQRWITLFGFQFQPSEIAKLAVIAMLAAHLSEIQRGLRLE
                     DVWRATGLVALPMGLVFLQPDVGTSMAFAAILLGVLVVAGAKAKHLGVLTLASIFAVF
                     LAFRVGIVQEYQLARLRAFIDPSADPLRAGYNIEQSRIAIGSGGLGGKGYLQGTQTNL
                     DFVPQQHTDFVFTVVGEEFGFLGGMLLLLLFALLLWRGIRIAMLSKDAFGTLLATGIV
                     SVVAFQVFVNVGMTLGIMPVTGIPLPFVSYGGTALVTNFLAVGLLMNIHMRRFK"
     gene            complement(3778..5673)
                     /gene="mrdA"
                     /locus_tag="VM638_06905"
     CDS             complement(3778..5673)
                     /gene="mrdA"
                     /locus_tag="VM638_06905"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVM12185.1"
                     /translation="MTEGKAAARLRVLAWAVVVMFGALMTRMWFLQVMASDEYRQAAD
                     DNRVQVAPIPAARGRILDRNGDLLVQNRTSRVVTVKRSDVENEEALLLKLSRVLGVDA
                     DELGERLDDPDYGPFEAIPVAEDVPEETILYLAERQREFPGVDYGTIGVRDYPNGSLA
                     AHILGYLGQINEQELHTPPFRGYRPGQAVGRGGVEQMYERYLHGKDGHQAYEVDARGR
                     RMGRLKGTAPVEGNDVVLTIDRKIQKAAEEALSEGVSSARQIVHEESGSYLKAAAGAV
                     VVLDARDGEVLAMASYPTYDPRAFLEGLTQREWDRLTSKRRNFPLNNRAIQGQYPPGS
                     VLKPFVAAAAVKGGYAHPNGFYPCPPEFSVEGDTSNTVFRNWKDTDSGTISFGQALVE
                     SCDTVFYRFGLDFYRERKIRGELLQQHLRAWGYDRPTGIDLPSEVKGRVPDAAWKSIV
                     HSKHPDLFPDPVWYPGDTINMSIGQGDVLATPLQVARSFAALANGGTLVRPHVGLRVQ
                     DPKGNVVRRLRPAPGPRVRYGRKLLDRIEDALEGVVTSGTGAHAFSGFPVPVAGKTGT
                     SETPNQQPHSWFAAFAPADRPRYVAVAVVEEGGHGSEVAAPIVRRIMEHLFDVDPAPF
                     RVGIAAD"
     gene            complement(5678..6184)
                     /gene="mreD"
                     /locus_tag="VM638_06910"
     CDS             complement(5678..6184)
                     /gene="mreD"
                     /locus_tag="VM638_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HVM12186.1"
                     /translation="MRRSLAWTAVVVTALLLQSTAFAQIKLLGAKPELMYLLTVVIAM
                     LEGPASGTISGFAAGMGQDFLLHQPKGITALTLTLLGYVVGMIRQYIVTPSPALPVVL
                     VAAATFVGVLFYGLLSFLLGQLTVGFLFLLRTAFLSAIYNAVLTPILYPVIRRVAEAS
                     RPKKVFRW"
     gene            complement(6212..7063)
                     /gene="mreC"
                     /locus_tag="VM638_06915"
     CDS             complement(6212..7063)
                     /gene="mreC"
                     /locus_tag="VM638_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HVM12187.1"
                     /translation="MAVHARARSTRLLVVSLVFASLMTITIDFRGGERGPLAAVGRAS
                     LAVITPLQEAVSTIFRPIGAFFSAIGSISDLRSENARLRAELQRLSGEQAQILELQRE
                     NDTLRRNLDLRERLGFQTRGATVIGESVSNFEWAVIVDRGSDDGVRIDTPVIAAEGLV
                     GRVVRVSRSSAKVLLIVDPRSRVAVRLAGSGEQGALVGRRAGQDLRLDLVDPLTEVKP
                     GEQVLTSGLEGGIFPPGVPVGVVSEVLPSEVDLTKHVLVRHAVDFSRLDHVQLVIPDR
                     AAGSLGR"
     gene            complement(7087..>7448)
                     /locus_tag="VM638_06920"
     CDS             complement(7087..>7448)
                     /locus_tag="VM638_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVM12188.1"
                     /translation="EPEAEIRGRDMVSGLPRTVVVSSAEVRQALEEPLHSIVDAVRGT
                     LDRCPPELAADIMDKGVVLTGGGALLRGLDERLQHETGMPIHVAESPLYCVAIGSGKC
                     LEEFEVLKRVLISSGRR"
BASE COUNT         1100 a         2673 c         2563 g         1112 t
ORIGIN      
        1 gtggtcttct tggcgtcctc gcccgagccc gtcgcggcct cctgggccgg ctccggcttg
       61 cgcaggacct tgtcgcccac gacgatctcc tcgatctcca gctcggtcat gagctggcgg
      121 tggccctggg tccgggcgta cccgctcttg ttccggtact tgaagacctt gatcttgtcg
      181 cccttcttct cgccgacgag gcgtccgacg accttgaccg tgccgccctt cgggcgatgc
      241 accgtcccgt cctccatgac gaagagcgtg tcgagctcca ccgtcgaacc ggggtcgagc
      301 ttcttcatgt actcgacctc gatcacgtca ccgggctcca cccgctgctg ctttccgccc
      361 acccgcacga ctgcgtacat cgaccctcct agttcccctc cgtgtccagg ctgacgagga
      421 taccccgccc ctcgcacgtg gggcaggtct gcgagaacga ctcgaccagc ccggccgaca
      481 cccgcttccg cgtgacctcg aggagcccga gcggcgtgat atcgaacacc tgcgagcggg
      541 tcttgtcctg cgccatctcc tccttcaacg cccggatcac cttgttccgg ttcgcctgca
      601 ggagcatgtc gatgaagtcg atcacgatga tcccgccgat gtcgcgcagc cgcagctgcc
      661 tcgcgatctc ccgcgccgcc tcgaggttcg tctggacgac cgtctcctcc aggttcgtct
      721 tgccgaccgc cttcccggtg ttcacgtcga cgaccgtcat cgcctcggtc cggtcgatga
      781 tcaggtaacc accggacggc agccacacct tccgctcgag cgccttgtgg atctgctcgt
      841 gcacgtggta ctcctcgaac accgggagcg gcccctggtg cagccgcacg cggtcgagga
      901 tgtcgggcgc gacctccttc aggtacgccg tcacgcgctc gtagacgctc tcggagtcgg
      961 tgacgacctc gcggaactcg acggccgaga agacgtcgcg gaggacgcgg atcgtcagct
     1021 ccggctcctc gtagaggacc ttcggcggct tcccgcgtcg cgacttgcgg cggatcgact
     1081 cccacgtctt cttcaggcgc tcgaggtcct cctcgaggcc ttcgggggtg gcgccctcgg
     1141 cggccgttcg gatgatcacg ccggcgcccg acgggcggat ctccttcatg atcccccgca
     1201 ggcggttccg ctccttctcc ggaaggcgcc ggctgatccc cgacgcgctg ccacccggga
     1261 cgtacacgag gtaccggccc gcgaggctga tctgaccggt caggcgcgcg cccttgccgc
     1321 ccatcggatc cttcgtcacc tggacgacga tcggctgccc cggcttcagg acctgctcga
     1381 tccggcgcgg ctgctcctcc acgtcctcgt cgtgggagac ctcgccggcg tagaggacgg
     1441 cgttgcgccc tcggccgaga tcgatgaagg ccgcctccat ccccggaagg acgttctgca
     1501 cccgcccgag gtagacgttc cccaccatcg accgggctcc cgcacgggtg acgtagtgct
     1561 cgacgagctc acggccttcg gcgacggcga tctgatcgcg gtcgccgttc tcggtgatca
     1621 gcatgatctt gtcgaggcga cgctccggac ggatctccgg ctcgcaccgg cgccgggtca
     1681 cgacgccgcg acggcggcgc gcggtgcggg tcgccggggg ggcctcgtct tccttctgct
     1741 tcggcttcgc ccgagagcgg cggccgtccg cgggcttcga ggcgggcttc gtgtcgggct
     1801 tcgcctcgct cgacggatcg gccgcggcct cgccgtcccc cgagtccgag ggcttcttcc
     1861 ggcggcgccg gcggcgccgg cgcttcgtcg acgagctggt ccccccctcc ggggcgtcgg
     1921 aagcctccgt cgggtccgcg cggtcgtccg catccgctgc gtccgatgcg gcaccgggcg
     1981 atgcatcccc cggcgggtcg gacgtcaccg cggtggaacc ctcggaggcc acatcgccgt
     2041 cctcgctcgt ggtcgccggc ttcctcgacg agggccgccg gcggcggcgg cgcgagcccg
     2101 acgaggtgga gcgaggaccc gatccctccg cgcccggttg gtcgccgtcc gcggccgcgg
     2161 ccccgtcgcc ggaaccgctc tcggtctgct ccggcggtgc ttcgcccgtg gtggggggag
     2221 cggcgtccgc gggtgcctcg gccgtgcccg cagcgcgctg ggcctgggag cgggagcctc
     2281 taccccgtcc gcggccaccg cggcggcgcc gcggacgtgg ggtggatggt tcggaggcct
     2341 cctgctcggg ggcctgccgt tcggcggacc cctcctcggg agcccgtgct tcctctgtca
     2401 tgacgtggtc ctttcgctcg ggagcgggtc gctcccagcc ggaggggcca cgcgccgccg
     2461 gcgcgggcct cacctccggg cacggatgca ggtgggtggt gcgaacgccc gttcgacggg
     2521 aaggcggacg cgcggggtcg gcggcccgga acggggtcgt ggaacgaaga catctgagcc
     2581 caggatagca ggcgggaccc cccgcccccc tctacttgaa tcgccgcatg tggatgttca
     2641 tcagcaggcc gacggccagg aagttcgtga ccagcgccgt ccccccgtac gagacgaagg
     2701 gcagcgggat cccggtgacg ggcatgatcc cgagcgtcat cccgacgttg acgaagacct
     2761 ggaacgcgac gaccgaaacg atcccggtgg cgagcagggt cccgaacgcg tccttcgaga
     2821 gcatcgcgat ccggatgcct cgccagagca ggagggcgaa cagcagcagc aggagcatcc
     2881 cgccgaggaa gccgaactcc tcccccacca cggtgaagac gaagtcggtg tgctgctggg
     2941 gcacgaagtc gaggttggtc tgggtcccct ggaggtagcc cttcccaccg aggccaccgg
     3001 acccgatcgc gatccgcgat tgctcgatgt tgtagcccgc ccggagcgga tcggcggaag
     3061 gatcgatgaa cgcccggagc cgggcgagct ggtactcctg gacgatgccg acccggaacg
     3121 ccaggaagac ggcgaagatc gacgcgagcg tcagcacacc gaggtgcttc gccttcgcgc
     3181 cggcaacgac gaggaccccc aggaggatcg cggcgaacgc catcgacgtc ccgacgtccg
     3241 gctggaggaa gacgaggccc atcggcagag ccacgagccc cgtcgcacgc cagacgtcct
     3301 ccaaccgcag cccgcgttgg atctcggaga gatgcgccgc cagcatcgcg atcaccgcga
     3361 gcttcgcgat ctccgacggc tggaactgga acccgaacag cgtgatccag cgctgcgccc
     3421 cgagcgtgcg gttccccagg ggcgtcatca cgagcagcag caggatcacg accgcgccgt
     3481 acgtgatcgg cgcatagacc ttcgcgaggc ggtagtcgaa cgtcgcgacg aggatcatcg
     3541 cgatcagacc caggagcatg aacgcgaact gcttcttcat gaagagcccc gggtcggccg
     3601 tccggtcgaa cgcgcggatg ctctggtgcg tcgcggagta gatcagcatc aggccgtaga
     3661 tcgtcagcgc gagcgtcgtg accacgagca tcgggtcgag gtggcggagc ggcgaccgct
     3721 ccgaggccag caacctcccg gcgccgacgt cgagcgagcg accggcgctg acgtccatca
     3781 gtccgccgcg atcccgaccc ggaacggggc cgggtcgacg tcgaacaggt gctccatgat
     3841 ccggcggacg atcggcgcgg ccacctcgga cccgtggccc ccttcctcga ccaccgccac
     3901 ggccacgtac cgcggccggt cggcgggagc gaacgccgcg aaccacgagt ggggctgctg
     3961 gttcggcgtc tccgaggtcc cggtcttgcc ggcgacgggc acggggaacc cgctgaaggc
     4021 gtgagccccg gtgccgctgg tgacgacccc ctcgagggca tcctcgatcc gatcgagcag
     4081 cttccgcccg taccgcaccc gcgggcccgg cgccgggcgg agccgccgga cgacgttccc
     4141 cttcgggtcc tggacgcgca gcccaacgtg gggccggacg agcgtcccgc cgttggcgag
     4201 ggcggcgaac gaccgcgcga cctgcagcgg cgtcgccaag acgtcgccct ggccgatcga
     4261 catgttgatc gtgtcgcccg ggtaccagac ggggtcgggg aagaggtccg ggtgcttcga
     4321 gtgcacgatc gacttccacg cggcgtccgg cacccggccc ttgacctcgg acgggaggtc
     4381 gatcccggtg gggcggtcgt agccccacgc gcgcaggtgc tgctgcagca gctccccgcg
     4441 gatcttccgc tcgcggtaga agtcgaggcc gaagcggtag aagacggtgt cgcaggattc
     4501 gaccaaagcc tgcccgaagg agatcgtccc cgagtcggtg tccttccagt tccggaagac
     4561 ggtgttggac gtgtccccct cgacggagaa ctccggcggg cacgggtaga acccgttcgg
     4621 gtgggcgtac ccgcccttca ccgcggccgc ggcgacgaac ggcttcagca ccgatcccgg
     4681 cgggtactgc ccctggatcg cacggttgtt gagcgggaag ttccgacgct tgctggtgag
     4741 ccgatcccac tcgcgctgcg tcagcccctc gaggaaagcg cgggggtcgt acgtggggta
     4801 ggaagccatc gccaggacct cgccatcccg agcatcgagc acgaccacgg cgcctgcggc
     4861 cgccttgagg taggagcccg actcctcgtg cacgatctgg cgagcgctcg agaccccttc
     4921 ggagagcgct tcctccgcgg ccttctggat cttgcggtcg atcgtgagga cgacgtcgtt
     4981 gccttcgacc ggcgctgtgc ccttcaggcg gcccatcctc cgcccgcgcg cgtccacctc
     5041 gtaggcctgg tgaccgtcct tgccgtgcag gtaccgctcg tacatctgct ccacgccgcc
     5101 gcggccgacc gcctgccccg gtcggtaccc gcggaacggc ggggtgtgga gctcctgctc
     5161 gttgatctgc ccgaggtacc ccaggatgtg cgccgcgagc gatccgttcg gataatcgcg
     5221 caccccgatg gtcccgtagt cgacacccgg gaactcccgc tggcgctcgg cgagatagag
     5281 gatcgtctcc tcggggacgt cctcggccac cgggatcgcc tcgaaggggc cgtagtccgg
     5341 gtcgtcgagg cgctcgccga gctcgtccgc gtccacgccg agcacgcggg agagcttgag
     5401 caggagcgcc tcctcgttct cgacgtcgct gcgcttgacc gtcacgaccc ggctcgttcg
     5461 gttctgcacg aggaggtccc cgttgcggtc gaggatgcgc ccgcgagccg ccgggatcgg
     5521 ggcgacctgc acccggttgt cgtcagccgc ctggcggtac tcgtcagacg ccatcacctg
     5581 gaggaaccac atccgggtca tgagcgcgcc gaacatcacg accacggccc aggcgaggac
     5641 gcgcagccgc gcggctgcct tgccttcggt catgggatca ccaccggaac accttcttgg
     5701 gcctcgacgc ctccgccacg cggcggatca ccgggtacag gatgggggtc agaaccgcat
     5761 tgtagatggc cgaaaggaaa gccgtccgga gcaggaacag gaacccgacg gtcagctggc
     5821 cgagcaggaa cgacagcagc ccgtagaaca ggacgccgac gaaggtcgcc gcggcgacga
     5881 ggacgaccgg cagggcgggg ctcggcgtca cgatgtactg gcggatcatc cccacgacgt
     5941 agccgagcaa cgtgagggtc agcgcggtga tgcccttcgg ctggtgcagg aggaagtcct
     6001 ggcccatccc ggccgcgaac ccgctgatgg tgcccgaggc cgggccctcg agcatcgcga
     6061 tcacgaccgt caggaggtac atcagctcgg gcttcgcccc gaggagcttg atctgggcga
     6121 aggcggtgga ctgcagcagg agggcggtca cgaccaccgc cgtccacgcg agggagcggc
     6181 gcaccgccca agtctactcc gcgcgttccg gctaccgacc gagcgagccg gccgcgcgat
     6241 cggggatcac gagctgcacg tggtcgagcc gggagaagtc gaccgcgtgc cggacgagca
     6301 cgtgtttcgt gaggtcgacc tccgagggca ggacctccga cacgacgccg acgggaacgc
     6361 ccggcgggaa gatgccgcct tcgaggcccg acgtcagcac ctgctcgccc ggcttcacct
     6421 cggtgagcgg gtcgacgagg tcgaggcgca gatcctgtcc ggcccggcgg ccgacgagcg
     6481 caccctgttc cccgctcccg gcgaggcgca ccgccacgcg cgaccgcggg tcgacgatca
     6541 acaggacctt cgcggacgaa cgcgagacgc gcacgacgcg cccgacgagc ccctccgcgg
     6601 cgatcacggg ggtgtcgatg cgcacgccgt cgtccgaccc gcggtccacg atgaccgccc
     6661 actcgaagtt cgagacgctc tccccgatca ccgtcgctcc ccgcgtctgg aaccccagcc
     6721 gctccctcag atcgaggttc cgccgcagcg tgtcgttctc acgctgcagc tcgaggatct
     6781 gcgcctgctc ccccgagagg cgctgcagct cggcgcgcaa gcgcgcgttc tcggagcgga
     6841 ggtcgctgat gctgccgatc gccgagaaga acgcgccgat cggccggaag atcgtcgaga
     6901 ccgcttcctg gagcggggtg atcacagcga gcgaggcccg tccgacggcg gcgagcggac
     6961 cacgctctcc gccccggaag tcgatcgtga tcgtcatcag gctcgcgaac acgagggaga
     7021 cgacgagcag gcgcgtgctc cgggcgcgtg catgaaccgc cacgggggga gagtctagcg
     7081 cggccgctag cggcggccgg aggagatcag gacccgcttc agcacctcga actcctcgag
     7141 gcacttcccg gatccgatcg cgacgcagta cagcgggctc tcggcgacgt ggatcggcat
     7201 gccggtctcg tgctggagcc gctcatcgag accgcggagc aacgcgccgc cgcccgtgag
     7261 caccacgccc ttgtccatga tgtcggcggc gagctccggc gggcatcggt cgagcgtgcc
     7321 gcgcacggcg tcgacgatgc tgtggagcgg ctcctccagc gcctggcgga cctcggcgct
     7381 cgagaccacg acggtgcgcg gcaggccgga gaccatgtcg cggccgcgga tctcggcctc
     7441 gggctcct
//
LOCUS       DATLGG010000241         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_120203, whole genome shotgun sequence.
ACCESSION   DATLGG010000241 DATLGG010000000
VERSION     DATLGG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120203"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VM638_06925"
     CDS             complement(<1..300)
                     /locus_tag="VM638_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM12189.1"
                     /translation="MDFDLTEEQQEFRAAVRSFAEEVIGPRAEEMDRAGELPMDIVKQ
                     MGELGLFGLPFPEEYGGQGADFLTFCLAIEEIARVDSSMAITLEAAVGLGANPVFH"
     gene            complement(320..1093)
                     /locus_tag="VM638_06930"
     CDS             complement(320..1093)
                     /locus_tag="VM638_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVM12190.1"
                     /translation="MPASRRSLVIVLGVAAAVLIPAGLLVQTAVLGGRVARTERVVQD
                     EPAQPEIPPLPPCGYGRERTAYRGYEDWQRTLLDTTYRLPRSYEPPDLVPISRLGLGT
                     DEMLVRELVIDDLAALAAAAEEAGNPIDVTWAYRSFTTQRQVLETWTQQRGRDEALRI
                     AARPGHSEHQLGTGLDFKTRGAPNVDVGWRSEPAGRWMRQHAWEFGFIETYPKDAEAL
                     TCYDHEPWHYRYFGRDVARQIRESGLTAREFLWRLQPHG"
     gene            complement(1097..2020)
                     /locus_tag="VM638_06935"
     CDS             complement(1097..2020)
                     /locus_tag="VM638_06935"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HVM12191.1"
                     /translation="MEVLQGTGSLPIDGSSAVTIGFFDGVHRGHQAVIGRTVEVARRE
                     GFRSVAVTFDRHPREVLTPHEVPALLTTLRRKAGLIEELGVEVLVVHPFTEEVARSSA
                     DAFVSRILAGALRAAHVVVGESFTFGHRAAGTLATLVEHGAAHGFTAEGVPLLRLDGR
                     VVSSTSIREALATGDLDWPHRALGRRHVVDGTVVPGAGRGKGLGFPTANLRVPDGIRL
                     PGRGVYAGRASNDRGSWPAAINIGINPTFGEEPLHLEAHLLGFDGSLTGQVLAVEFWE
                     RLRDEVRFASADELSRQIAADVERTRALVEV"
BASE COUNT          322 a          811 c          804 g          289 t
ORIGIN      
        1 gtggaagacc gggttcgcgc cgaggccgac ggcggcctcg agcgtgatgg ccatcgagga
       61 gtcgacgcgc gcgatctcct cgatcgcgag gcagaacgtg aggaagtcgg cgccctgccc
      121 gccgtactcc tccgggaacg gcaggccgaa aagaccgagc tcccccatct gcttgacgat
      181 gtccatcggc agctcgccgg cgcggtccat ctcctccgcc cgcggcccga tgacctcctc
      241 ggcgaacgag cggacggccg cgcggaactc ctgctgttcc tcggtgaggt cgaagtccat
      301 gccgagaggg tagccgactt cagccgtgcg gctggagccg ccacaggaac tcgcgggccg
      361 tcaggccgct ctcgcggatc tgccgcgcga cgtcgcggcc gaagtaccgg taatgccacg
      421 gctcgtggtc gtagcaggtc agggcctcgg cgtccttcgg gtacgtctcg atgaacccga
      481 actcccaggc gtgctggcgc atccagcggc cggccggctc gctccgccag ccgacgtcga
      541 cgttaggggc gccgcgcgtc ttgaagtcca gaccggtccc cagctggtgc tcggagtggc
      601 ccggccgggc cgcgatgcgc agcgcctcgt cgcgcccgcg ctgctgcgtc cacgtctcga
      661 gcacctggcg ctgggtggtg aagctccggt acgcccaggt gacgtcgatc gggttgcccg
      721 cctcttccgc cgccgcggcg agggcggcga ggtcgtcgat cacgagctct cggacgagca
      781 tctcgtcggt cccgagaccg agccgggaga tcgggacgag gtcggggggc tcgtacgacc
      841 gcggcagccg gtaggtcgtg tccagcagcg tccgctgcca gtcctcgtac ccacggtacg
      901 ccgttcgctc acggccgtac ccgcagggag ggagcggcgg gatctccggc tgggcaggct
      961 cgtcctggac cactcgctcg gtccgcgcga cccggccacc gaggaccgcc gtctggacca
     1021 gcagcccggc ggggatgagg acggcggcgg cgacgcccag cacgatgacg agcgatcgcc
     1081 gggacgccgg catccgctac acctccacga gcgcgcgcgt ccgctcgacg tccgcggcga
     1141 tctgccggga gagctcgtca gccgaggcga agcggacctc gtcgcgcagg cgctcccaga
     1201 actccaccgc gagcacctgc ccggtgaggg agccgtcgaa gccgaggagg tgggcctcga
     1261 ggtgcagcgg ctcttccccg aacgtcgggt tgatgccgat gttgatcgcc gccggccacg
     1321 accctcggtc gttggacgcg cggcccgcgt agaccccgcg ccccggcaga cggatcccgt
     1381 ccggcacgcg caggttcgcg gtcgggaacc cgaggccctt cccccgtccc gctccgggca
     1441 ccacggtccc atcgacgacg tggcgccgcc cgagggcccg atgcggccag tcgaggtcgc
     1501 ctgtcgccag cgcctcgcgg atcgaggtgg aggagacgac gcgcccgtcg agcctcagca
     1561 gcggaacacc ctcggcggtg aagccgtgcg ccgctccgtg ttcgaccagc gtcgccagcg
     1621 tccccgcggc tcggtggccg aaggtgaagc tctctcccac cacgacgtgc gcggcgcgga
     1681 gcgccccggc caggatgcgc gacacgaagg cgtccgcgga cgagcgcgcc acctcctccg
     1741 tgaacggatg gacgacgagg acctccaccc cgagctcctc gatcagcccc gccttccgcc
     1801 gcagcgtcgt gagcagggct ggcacctcgt gcggggtcag gacctcccgc ggatggcggt
     1861 cgaacgtgac ggccacggag cggaatccct cgcggcgggc gacctccacg gtccggccga
     1921 tcaccgcctg gtggccccgg tgcaccccgt cgaagaaccc gatcgtcacg gcgctcgaac
     1981 cgtcgatcgg aagggagccg gtgccctgga ggacctccat cagggcagga gcgtacagga
     2041 cgtcaccgcg gtgacggccc gggaagcggg gcgcctagat ggccgaggcg gcgaggccct
     2101 cgatccggac cgggtcgccg cgagaggtga cgacgatccg gaccgtccca gagcgtgggc
     2161 ggctccagga ggcgacctcg atgatgcgca gcttcctcgt cacggcggac cggaggctgg
     2221 atcggg
//
LOCUS       DATLGG010000242         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_171872, whole genome shotgun sequence.
ACCESSION   DATLGG010000242 DATLGG010000000
VERSION     DATLGG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171872"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VM638_06940"
     CDS             complement(<1..188)
                     /locus_tag="VM638_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12192.1"
                     /translation="MEERRGSAPFPIRIFAIALIGVIVVVGLLLWNLLDFQRRSDALH
                     ERHRALAENIGRIRLIDEA"
     gene            complement(282..1328)
                     /gene="add"
                     /locus_tag="VM638_06945"
     CDS             complement(282..1328)
                     /gene="add"
                     /locus_tag="VM638_06945"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HVM12193.1"
                     /translation="MATLDEALARLPKAELHCHIEGTMRPATVVELARKNGVELPAED
                     PEELYRYTSLDSFLEVFWLVQACLADRADWARLAYESLVEAAEHGVVYRETFFTPARH
                     LAAGQRLADIVAGLEEGLEAAERETGARAMLIAGIDKAYGGEAGRQLVEELIELKRSG
                     GAERVIGIGMDSTEQGNPPSRFVDAYRLAAGFGLRRTGHQGETTEAGWIRDALDGLGL
                     ERIDHGIRVLDEPGLVRRLAEEGLPFTVCPTSNVRIANAVDRLEDHPFPSMRDAGLHV
                     TINTDDPAMIDLDPAREYAACARAFGYGFDDMVRIARAAFEAAWLSAAERADLLKRLD
                     RDADLLRREMARAG"
     gene            1362..2267
                     /locus_tag="VM638_06950"
     CDS             1362..2267
                     /locus_tag="VM638_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVM12194.1"
                     /translation="MRRSDPRGLGVVVVAAAFFAAATVMARLAYAAGADPPIVVALRF
                     VVGAAVLWVVVAIGRRRARLRRNDALWMVAMGAVSVVVGLLFFAAVQRLGAGPGTLTL
                     YAHPALVAGVAAVLRWERFGPLKAVALLVGLGGVALVVGTPAGGIDAVGVLLGLGAAV
                     ALAVYVLQAKRAGEGVEPVVAGAWVLSSAAVVFVPPAVAGGLLDLGQTAAAWGWLAGL
                     GAATGAAFALFLKAVSLLGPSRASIGASAEPLLAVVFGALLLGERLSAIQLVGGTLVI
                     AAVALLPMVESRSVALGEAPPLGEG"
BASE COUNT          252 a          825 c          845 g          353 t
ORIGIN      
        1 gcctcgtcga tcaaccggat ccggccgatg ttctccgcga gcgcgcggtg gcgctcgtgg
       61 agcgcgtcgg accggcgctg gaagtccagg aggttccaca gcaggaggcc caccaccacg
      121 atgacgccga tgagggcgat cgcgaagatg cggatcggga agggcgcgct ccctcgcctc
      181 tcctccaccc tgcgatgcgt ctgttcgctg cgcgaggcgc tcaccatccc cggtccgtcg
      241 acgccgatca tcccacggcg atcgccgcgg ggatcggatg ttcagcctgc gcgggccatc
      301 tccctcctta ggaggtcggc gtcacgatcg aggcgcttga gcagatccgc acgctccgcc
      361 gccgacagcc aagccgcctc gaaggcggcg cgggcgatcc gaaccatgtc gtcgaacccg
      421 tagccgaagg cgcgggcgca ggcggcgtac tcgcgagcgg ggtcgaggtc gatcatcgcc
      481 gggtcgtcgg tgttgatcgt gacgtgcagc cccgcatcgc gcatcgaggg gaaggggtgg
      541 tcctcgaggc ggtcgacggc gttcgcgatc cggacgttgg acgtcggaca gacggtgaag
      601 ggaaggccct cctccgccag ccgacgaacg agtccgggtt cgtcgaggac ccggatcccg
      661 tggtcgatgc gctcgaggcc gagcccgtcg agggcgtcgc ggatccagcc cgcctccgtc
      721 gtctctccct ggtggccggt gcgccgcagg ccgaaccccg cggcgagccg gtaggcgtcg
      781 acgaacctcg agggcgggtt gccctgctcc gtcgagtcca tcccgatgcc gatcacgcgc
      841 tccgccccgc cggagcgctt cagctcgatc agctcttcga cgagctgacg cccggcctcg
      901 cccccgtagg ccttgtcgat gccggcgatc agcatcgcgc gcgcgcccgt ctcccgctcc
      961 gcggcctcga gcccctcctc cagaccggcg acgatgtcgg cgaggcgctg gccggcggcg
     1021 aggtgccgcg cgggcgtgaa gaaggtctcg cgatagacga ccccgtgctc agccgcttcg
     1081 acgagcgact cgtacgccag ccgcgcccag tccgcacggt cggcgagaca ggcctggacg
     1141 agccagaaca cctcgaggaa ggaatcgagg ctcgtgtagc ggtacagctc ctctggatcc
     1201 tcggccggga gctcgacgcc gttcttccga gcgagctcga cgacggtcgc ggggcgcatc
     1261 gtgccctcga tgtggcagtg gagctccgcc ttcggcaggc gcgcgagcgc ctcgtccagg
     1321 gtcgccatcg cggcgaggat gctatcccgt agcatcgccg cgtgcgaagg agcgacccgc
     1381 gcgggctcgg tgtggtcgtc gtcgcggcgg ccttcttcgc ggcggcgacc gtcatggcgc
     1441 gtctggcgta cgccgcgggc gccgacccgc cgatcgtcgt ggcgctccgc ttcgtcgtcg
     1501 gcgctgcggt cctgtgggtg gtcgtcgcga tcggccgccg ccgggcgcgg ctgcggcgga
     1561 acgacgcgct ctggatggtc gcgatgggcg ccgtctcggt cgtcgtcggg ctcctcttct
     1621 tcgcggcggt ccagcgcctc ggcgccggtc ccgggaccct gaccctctac gcccaccccg
     1681 cgctcgtcgc gggcgtcgcc gcggtcctgc gatgggagcg gttcgggccg ctgaaggccg
     1741 tggccctcct cgtcgggctc gggggtgtcg cgctcgtcgt cgggacgccc gcgggcggga
     1801 tcgacgcggt cggggtgttg ctcggcctcg gcgccgccgt cgcgctcgcg gtctacgtcc
     1861 tccaggcgaa gcgggcgggc gagggggtcg agcccgtcgt cgccggcgcc tgggtgctga
     1921 gctcggccgc cgtcgtcttc gtgcccccgg cggtcgcggg agggctcctc gacctcggcc
     1981 agacggccgc cgcctggggg tggctcgcgg ggctcggcgc cgcgaccggt gccgccttcg
     2041 cgctcttcct caaggcggtc tccctcctcg ggccatcgcg ggcttcgatc ggcgcgagcg
     2101 cggaacccct cctggcggtg gtcttcggcg cgctcctgct cggcgagcgt cttagcgcga
     2161 tccagctcgt cggcggcacg ctcgtgatcg cggcggtcgc gctgctgccg atggtcgagt
     2221 cgcgctcggt ggcgctcggg gaggcccccc cgctcggcga ggggtgacgc tcgct
//
LOCUS       DATLGG010000243         9917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_61626, whole genome shotgun sequence.
ACCESSION   DATLGG010000243 DATLGG010000000
VERSION     DATLGG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61626"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1629)
                     /locus_tag="VM638_06955"
     CDS             complement(295..1629)
                     /locus_tag="VM638_06955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVM12195.1"
                     /translation="MRPAPLPLVVTLATALAAGTGPALAQEPTLDEVVPTVRVEVAPP
                     VFSPNGDGRKDETTIAVTSDQDATLEVRILEGESPRRTFDPRAATAGRPETYVWNGRA
                     DGGRVDDGTYVVEAVARTLVGLEQSARTTVVVDTRRPRVAWKGLSPEPTDGGDPLRFR
                     FSLSDRPYPLEVRLEIRDSLGVVRAREERYAPGDRGLDWRPTYRNGGTLFPGLYRARL
                     RAVDAAGNRSVPQGRRFRVHRSVDAKVFHRLSGAGRRVAITIDDCHFADAWRRMLDTL
                     RRLRAKATFFCPGDRMMSFPGLVQRTVREGHTLGAHGWDHANMAGRTVASSNERLLAD
                     RRTAWKVARATTAPYFRPPYGSYDGAVIRAAGATSHPRVMMWDVDTNDTRRPGAAAIR
                     AAAVGRSRPGSVILMHTLDQSADALPGIIRGLRQRNLRPVTLPRLFRAAGYR"
     gene            1776..2954
                     /locus_tag="VM638_06960"
     CDS             1776..2954
                     /locus_tag="VM638_06960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVM12196.1"
                     /translation="MREAVIVEAVRTPVGRNGGKLAGWHPVDLLAEVLRSTVERTGID
                     PAGVDDHITGCVSQTGEQGINVGRNAWLAAGLPEEVPATTVDRQCGSSQQAIHFAAQG
                     VIAGAYDLVVASGVEHMTRVPMGVTMSQGPGIPFSQQLMDRYEGGLVPQGISAELIAE
                     RWKLTREQLDEFGLRSHRLAAAAIQEGRFQREILPIKLPADGGAEMFEQDEGVRFETS
                     IEKMATLPTPFKGDGVVTAGNSSQISDGAAAVVVTHPERARELGLRPRARLHSFALAG
                     VDPIIMLTAVIPATQRVLDRAGLRLEDIDVVEINEAFASVVLAWKRELQIPDAWFEEH
                     VNPNGGAIALGHPLGASGARLMTTLLHELERRDGRYGLQVMCEGGGMANATVIERLPA
                     "
     gene            complement(2962..3192)
                     /locus_tag="VM638_06965"
     CDS             complement(2962..3192)
                     /locus_tag="VM638_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12197.1"
                     /translation="MTHHAPNGDRTGYAFVKYAIILVIVIVVLFFLVRYVLPLLTGGD
                     EGNGQEEEPVFPSPPAQTQIVEIPTPDARLLV"
     gene            3247..3597
                     /locus_tag="VM638_06970"
     CDS             3247..3597
                     /locus_tag="VM638_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12198.1"
                     /translation="MRRSLTLAFTLVLGLSGTVMILLFIGSLGQLHPGNPFVAVVMGV
                     AFLLIGFGAVRAAVRPRVVEERSEPEDVEDLGVVFRCGDCGTELRVERLGELQVPRHC
                     GEPMLVERRPATRH"
     gene            complement(3591..3836)
                     /locus_tag="VM638_06975"
     CDS             complement(3591..3836)
                     /locus_tag="VM638_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HVM12199.1"
                     /translation="MPLYEYRCLECGSNVEVLHAMDGPAPVSCGECGGSLRRVWSRVG
                     IRFSGWGFSSTDALLEGSGPRRDFRAVRERAEEIADQ"
     gene            complement(3914..4147)
                     /locus_tag="VM638_06980"
     CDS             complement(3914..4147)
                     /locus_tag="VM638_06980"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HVM12200.1"
                     /translation="MPKSKSKRRTYQPPPKPKPKPSPRWYAPVVLSILFAGVLVIVFN
                     YLGFLLPGSPRNLYLWVGLGMIGAGFIAATRLR"
     gene            4182..4478
                     /locus_tag="VM638_06985"
     CDS             4182..4478
                     /locus_tag="VM638_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12201.1"
                     /translation="MPRAASRAPLALAAATLGFLTVVAASQEPTPARATRRLGFVDLI
                     REQDERISTLRAEVSELERRVGRSRATASAGSCGRSDGSTSDRAGRPCAALESS"
     gene            4475..4870
                     /locus_tag="VM638_06990"
     CDS             4475..4870
                     /locus_tag="VM638_06990"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HVM12202.1"
                     /translation="MTLDDSPAGRSPTGDPNDLLVHERDIQTVVNALWSAGAEAVAVN
                     GERLTMRSAVRCAGNTLLLHGRVHSPPYRISAIGDARAIGGTLTAQPGMDRLLDAVAA
                     FGIRFDVETARVEIPGRDIASALEEAQPA"
     gene            4867..5013
                     /locus_tag="VM638_06995"
     CDS             4867..5013
                     /locus_tag="VM638_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12203.1"
                     /translation="MIAFLRRRQPRNPALAVVYWLAILAVTLAVLFIAFFYLDNYLPG
                     GGMF"
     gene            5022..5726
                     /locus_tag="VM638_07000"
     CDS             5022..5726
                     /locus_tag="VM638_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HVM12204.1"
                     /translation="MAAVDAYAREVEARVEAAGSDGLVLDRTVFYPRGGGQPGDTGMI
                     AWEGGEARVVDTVRSGGVPLHVIDGPRPPDGVIVRGTIDWDRRHLTMRTHTALHALSG
                     IIWRDYDAKVTGGNMEPGAARMDFELAAISADFGREVEEKLNAALAEDHPVEVRFLPR
                     AEALADPDLIRTKVSLIPEHVDPIRIIDIVGLDKQADGGTHVRSTAEVGRVRVVKTDS
                     KGKGFKRIRIALDPLG"
     gene            5794..7527
                     /locus_tag="VM638_07005"
     CDS             5794..7527
                     /locus_tag="VM638_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVM12205.1"
                     /translation="MAERSWLTSYPPEVPHSLEPYPERSLYSVLEGSVQRFPDQPAIA
                     FFGKRLTYAELEREVVRFAGVLASMGVRKGDRVGFVLPNCPQYVIAFFACQRLGAVVV
                     GNNPLYTKRELAHQLNDAGVETLIVLDLLYPLVSSVRNEVGFRRVIVTKMTDYMPFPL
                     NVLAPIKFRKEAKHEGKGWPPVPEDADADWWADLMKQDHPEPPVAQVDAKEDLAALVY
                     TGGTTGVSKGAMLTHFNMVSNTLQTASWFKGAGEEGKGGMMCVLPFFHSYGLGAVMNV
                     GIVLGLKLVLLPRFELKMALKAIQKEKPTLFPGVPRIYIAINESPETTKFDLTSIVAC
                     FSGAAPLPVAVAEKFEQITGGRLVEGYGLSETSPVTHINPIFGKRKFGSIGTPLPDTD
                     CKLVDVEDPDVEVSGDQEGELCISGPQVMKGYWNRPDETAGMIRQHADGKLWLHTGDI
                     AKMDEEGYFSIVDRKKDMVIVSGFNVYPTEVEQVLYRHEKVQKVAVVGVPDDTTGEAV
                     KAYVVLREGVTATPEEIIEWARNPEHGLTGYRVPKHVEFRDELPETMVGKVLRRVLID
                     EEKQKAAASSS"
     gene            complement(7524..7676)
                     /locus_tag="VM638_07010"
     CDS             complement(7524..7676)
                     /locus_tag="VM638_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12206.1"
                     /translation="MTRITEVPLMAALAALGLASLLFAWRSFVLLYRDIRPKYRGSHR
                     RRRSRA"
     gene            complement(7673..9277)
                     /locus_tag="VM638_07015"
     CDS             complement(7673..9277)
                     /locus_tag="VM638_07015"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HVM12207.1"
                     /translation="MTLWFVEEGFTPEERTILARHFTNVDGPVFALVNLPEVTKGALF
                     ARYSRSPKSLRRLFLDEFADETEETTSAGAGVERAERLYERVFVEYGDDSVAQLGGVH
                     LAAEQSSQILAKVLERGRLMAYLEQSTRYVPYDDRPGGRWRYAEPPEVAEAGLSERYR
                     TTLDAAFETYARWLEPMQDHLRRLFPQDPSDPDPVYRATIRAKACDVLRGVLPAATRT
                     NVGIFGTGQSYEQLLLRMRAHPLAEVRAYADLILVELRKVIPAFLRRVDLPDRGGRWS
                     RYLDETRRSLTAAAEELVREPAEPRPEVVLTDFDPQGEIKVVAAALYEVTDLPDDQLL
                     RRARDMTVGERMRVLRTYVGERSNRRHKPGRAFERTGYRFDVLCDYGAFRDLQRHRLL
                     TIEWQELTPGHGADLAPEIEEVGAGEEVEASLSACADLWKTLRDAGLGLAAQYAVPMA
                     CRIRFVMQMNAREAMHLIELRTTPQGHPAYRRVAQEMHRLIGEQAGHRAIAEAMRFVD
                     HSSVGLERLEAERRADQRRRDAGTPT"
     gene            complement(9274..>9917)
                     /locus_tag="VM638_07020"
     CDS             complement(9274..>9917)
                     /locus_tag="VM638_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HVM12208.1"
                     /translation="PDVIGEPQGRAERILERAGFTVRVREEPHDQVEEGRVFDQNPKP
                     ETSAPENSEVTILVSTGPEPVEVPNLIGKSLDDARADLQAAGLRLGSVERVESEEAPD
                     TVLDQSPDAGREVRPGREVNVVVSRGITTVVVPRVDDCVDEGTAQARLEDAGLQMRVV
                     GEEVTTACPAGRIFRQDPGAGESVQAGSRVRVWKSAGPPPTSPSPTATASPSP"
BASE COUNT         1458 a         3454 c         3577 g         1428 t
ORIGIN      
        1 gggcaggccc gcgagaccga tgtccttcat cggccgccga gcctagcagg ggctcaggcg
       61 tcccacgcat gaccgcagcc gcgacagacg tgatggccga gcgtgatccc gagccgggtg
      121 cttccgcacc tgggacaccg atcgagacgg tccgtccagt ccgggcgctc ggtctgacac
      181 gacgggcaga cgaggacctg cctgcccccg gcgacgccgc gcgcccacgg tcgctcgcgc
      241 ttgtcggggt cgtagggtgg gcgcccgcac agcaggcagg acgggctcgt cacgtcagcg
      301 gtagccggcg gcccggaaga ggcgagggag cgtcaccgga cgcaggttcc gctggcggag
      361 gcctcggatg atccccggga gcgcgtccgc gctctggtcc agcgtgtgca tcaggatcac
      421 gctccccggg cgcgatcgtc cgaccgccgc ggcccggatg gctgccgccc cggggcgccg
      481 cgtgtcgttc gtgtccacgt cccacatcat cacccgcggg tgcgaggtgg cgccggccgc
      541 acggatcacg gccccgtcgt aggagccgta cggcggtcgg aagtacgggg cggtcgtcgc
      601 ccgcgccacc ttccaggccg tgcgccgatc cgcgaggagc cgttcgttgg aggacgcgac
      661 cgtccgtcct gccatgttgg cgtggtccca tccgtgcgcg ccgagggtgt ggccctcccg
      721 gaccgtccgc tggacgaggc ccgggaacga catcatgcgg tcgccgggac agaagaacgt
      781 cgccttcgct cggagccgcc ggagcgtgtc gagcatccgc cgccatgcgt cggcgaagtg
      841 gcagtcgtcg atcgtgatcg cgacgcgacg cccggctccc gacagccggt ggaacacctt
      901 cgcgtcgaca gagcgatgga cgcggaacct ccgcccctgc gggacgctgc ggttcccggc
      961 cgcgtccacg gcacgcagcc gagctcggta caggccgggg aagagcgtgc ccccgttccg
     1021 gtaggtcggt cgccagtcca gcccgcggtc gcccggcgcg tagcgttcct ccctcgcgcg
     1081 aacgaccccg agggagtccc ggatctccag gcgcacctcg agcggatagg gccggtccga
     1141 gagcgagaat cggaaacgga gcgggtcgcc gccgtcggtg ggctccggcg aaagaccctt
     1201 ccatgccacg cgcggacggc gggtgtcgac cacgaccgtc gtcctcgcgc tctgctcgag
     1261 gccgaccagg gtccgcgcga ccgcctcgac gacgtaggtg ccgtcgtcca cccgaccgcc
     1321 gtccgccctc ccgttccaga cgtaggtctc gggcctgccg gcggtcgcgg ctcgggggtc
     1381 gaacgtgcgt cgtggtgatt cgccctcgag gatccggacc tcgagcgtcg catcctgatc
     1441 cgacgtcacc gcgatcgtcg tctcgtcctt gcggccgtcg ccgttcggtg agaagacggg
     1501 cggtgccacc tcgacccgca ccgtcggcac cacctcgtcg agggtcggct cctgcgcgag
     1561 ggcagggccg gtgcccgccg cgagggcggt cgcgagggtg acgaccaggg gcaggggggc
     1621 gggccgcatc gggccgagcc tacggccgcg ggcgccgtcg ggtccatagc catcgcgggc
     1681 cattccctga ggggtgcccc ggttgacgca tcttcgaagg gggcgtacgc tcgatatgtt
     1741 accgatgggt aggtaacgga gcgaggaggc agcggatgcg cgaggcggtg atcgtcgagg
     1801 ccgtgcggac cccggtgggt cgcaacggcg gaaagctcgc ggggtggcac cccgtcgacc
     1861 tgctggcgga ggtgctccgc tcgacggtcg agcggaccgg gatcgatccc gccggcgtgg
     1921 acgaccacat caccggctgt gtctcgcaga cgggggagca ggggatcaat gtgggccgca
     1981 acgcgtggct cgccgcgggc ctgcccgagg aggtgccggc gacgaccgtc gaccggcagt
     2041 gcggctcgtc gcagcaggcg atccacttcg ccgcgcaggg tgtgatcgcc ggcgcgtacg
     2101 acctcgtcgt ggcgagcggc gtcgagcaca tgacgcgcgt gccgatgggc gtgacgatgt
     2161 cccagggtcc cgggatcccc ttcagccagc agctcatgga ccgctacgag ggcggcctcg
     2221 ttccgcaagg gatctccgcc gagctcatcg ccgagcggtg gaagctgacg cgcgagcagc
     2281 tcgacgagtt cggtctccgc agccaccgtc tcgcggcggc ggcgatccag gaaggccggt
     2341 tccagaggga gatcctgccg atcaagctcc cggcggacgg cggcgcggag atgttcgagc
     2401 aggacgaggg cgtccgcttc gagacctcga tcgagaagat ggcgacgctg cccaccccgt
     2461 tcaagggcga cggggtcgtg accgcaggga actcgagcca gatctccgac ggcgcggcgg
     2521 ccgtcgtcgt gacccacccg gagcgggcgc gggagctcgg cctccggccg cgcgcgcggc
     2581 tccactcgtt cgcgctcgcg ggcgtggacc ccatcatcat gctcaccgcc gtgatccccg
     2641 cgacgcagcg ggtgctcgac cgcgcgggcc tgcgcctcga ggacatcgac gtcgtcgaga
     2701 tcaacgaggc gttcgcctcc gtcgtgctgg catggaagcg tgagctgcag atcccggacg
     2761 cctggttcga ggagcacgtg aaccccaacg gtggggcgat cgcgctcggc cacccgctgg
     2821 gagcgagtgg cgctcgcctc atgaccaccc tcctgcacga gctcgagcgc cgggacggcc
     2881 gctacgggct gcaggtcatg tgcgagggcg gcggcatggc caacgccacc gtgatcgagc
     2941 ggctccccgc ctgaggagcg ctcagaccag caggcgcgcg tccggcgtgg ggatctcgac
     3001 gatctgcgtc tgcgcggggg gcgacgggaa caccggctcc tcctcctggc cgttgccctc
     3061 gtccccaccc gtgagcagcg gcaggacgta gcggaccagg aagaacagca cgacgatcac
     3121 gatcacgagg atgatcgcgt acttgacgaa cgcgtagccg gtccggtcgc cgttcggtgc
     3181 gtgatgcgtc atggtcgccg cctacccgcg aggcccggca cgcaaacccg cggctagact
     3241 ggaagcgtgc gtcgctcgct gaccctcgcc ttcacgctcg tcctcggact ctcggggacg
     3301 gtgatgatcc tcctgttcat cggatcgctg ggtcagctgc acccggggaa ccccttcgtc
     3361 gccgtcgtga tgggggtggc gttcctgctg atcgggttcg gcgctgtccg cgcagcggtt
     3421 cgcccgcgcg tggtcgagga gcggagcgag cccgaggacg tcgaggacct cggggtcgtg
     3481 ttccgctgcg gggactgcgg cacggagctc cgcgtggagc gtctggggga gctccaggtg
     3541 ccgaggcact gcggggagcc gatgctggtg gagcggcgcc ccgcgacacg tcactgatcc
     3601 gcgatctcct ccgccctctc gcgcacggcc cggaaatccc ggcgagggcc gcttccctcc
     3661 aggagagcgt ccgtcgagga gaagccccac cccgagaacc ggatccccac gcggctccag
     3721 acccggcgca aggatccccc gcactcgccg caggacaccg gcgccggccc gtccatcgcg
     3781 tggaggacct cgacgttcga cccacactcg aggcagcggt actcgtacag aggcatgaga
     3841 cgagggtacc gcgacgccgt tgtccccacc tggggaaaca cctggggata attaccccgg
     3901 tgtcgttccg cccctagcgc aagcgggtcg ccgcgatgaa gccggcgccg atcatcccga
     3961 gccccaccca caggtagagg ttccggggcg accccggcag caggaagccg aggtagttga
     4021 agacgatcac gaggacgcct gcgaacagga tcgacaggac caccggcgcg taccagcgcg
     4081 ggctgggctt cggcttcggc ttggggggcg gctgataggt ccgccgcttc gacttcgact
     4141 tcggcacgag ccctccttcc gggtcggcct atgatacccg catgccgcga gccgcctccc
     4201 gagcccctct cgcgctcgcc gcggcgacgc tcggattcct gacggtcgtg gccgcgtccc
     4261 aggagccgac gcctgcccgc gctacccgcc ggctggggtt cgtggacctc atccgcgagc
     4321 aggacgagcg gatctccacc ctccgggcgg aggtctcgga gctcgagcga cgggtcgggc
     4381 ggtcgcgcgc gaccgcgtcc gcgggctcct gcgggcggtc cgacggctcg acgtccgatc
     4441 gggcgggtcg gccctgcgcg gccctggagt cgtcgtgacg ctcgacgaca gcccagcggg
     4501 acggtctccg acgggcgatc cgaacgacct cctcgtccac gagcgcgaca tccagacggt
     4561 cgtgaacgcg ctgtggtcgg ccggcgccga ggcggtcgcg gtgaacgggg agcggctgac
     4621 gatgcgctcc gccgtgcgat gtgccgggaa cacgctcctg ctgcacggcc gcgtccacag
     4681 ccccccgtac cggatctcgg cgatcggcga cgcgagggcg atcggtggga ccctcacggc
     4741 ccagccgggg atggaccggc tgctcgatgc cgtggcggcc ttcggcatcc ggttcgacgt
     4801 cgagacggcg cgggtggaga tccccgggcg cgatatcgcg agcgctctcg aggaggcgca
     4861 gcccgcgtga tcgctttcct gcgccggcgt cagccgcgga accccgcgct cgcggtcgtg
     4921 tactggctgg ccatcctcgc ggtcacgctc gcggtgctgt tcatcgcgtt cttctacctg
     4981 gacaactacc tgcccggcgg agggatgttc tgaccgagga gatcgcggcc gtcgacgcgt
     5041 acgcgcgtga ggtcgaggcg agggtcgagg ctgcgggctc cgacgggctc gtcctggacc
     5101 ggaccgtgtt ctacccgcgc ggcggtggtc agccgggaga cacggggatg atcgcctggg
     5161 aggggggcga ggcccgcgtc gtcgacaccg tgcggagcgg cggcgtgccg ctgcacgtca
     5221 tcgatgggcc acgcccacct gatggcgtca tcgtccgggg gacgatcgac tgggaccggc
     5281 gccacctgac gatgcgcacg cacacggcgc tccacgcgct gtcggggatc atctggcgcg
     5341 actacgacgc gaaggtcacc ggcgggaaca tggaacccgg agcggcccgg atggatttcg
     5401 agctggcggc gatctccgcg gacttcggcc gggaggtcga ggagaagctc aacgccgcgc
     5461 tcgccgagga ccatcccgtg gaggtgcggt tcctcccgcg cgcggaggca ttggcggacc
     5521 cggacctgat ccggacgaag gtgagcctga tccccgagca cgtcgacccg atccggatca
     5581 tcgacatcgt cgggttggac aagcaggccg acggcggaac gcacgtgcgc tccaccgccg
     5641 aggtcgggcg cgtgcgggtc gtgaagaccg acagcaaggg gaagggcttc aagcggatcc
     5701 ggatcgccct ggaccccctc ggctgacctg ccttgtcccg cgggcgcggg ttcgggttca
     5761 atgaccccac ggtcccgaaa ggagttggca tcgatggcgg aacgttcgtg gctgacgagc
     5821 tacccacccg aggtcccgca ctcgctcgaa ccctacccgg agcggtcgct ctactcggtg
     5881 ctggaagggt cggtccagcg gttccctgac cagccggcga tcgcgttctt cggcaagcga
     5941 ctgacgtatg cggagctcga gcgggaggtc gtgcgcttcg ccggggtgct cgcctcgatg
     6001 ggcgtgcgca agggggaccg cgtcgggttc gtcctgccga actgcccgca gtacgtgatc
     6061 gcgttcttcg cctgccagcg actgggcgcc gtcgtcgtcg ggaacaaccc gctctacacg
     6121 aagcgtgagc tcgcgcacca gctcaacgac gccggcgtgg agaccctcat cgtgctcgac
     6181 ctgctctacc cgctggtgag ctcggtccgc aacgaggtcg ggttccgccg cgtgatcgtc
     6241 acgaagatga cggactacat gccgttcccc ctgaacgtgc tcgcgccgat caagttccgc
     6301 aaggaggcga agcacgaggg gaagggatgg cccccggtcc cggaggacgc cgacgccgac
     6361 tggtgggcgg acctgatgaa gcaggaccac cccgagcccc cggtcgcgca ggtcgacgcg
     6421 aaggaggacc tcgcggcgct cgtctacacc ggcgggacga ccggggtctc gaagggcgcg
     6481 atgctcacgc acttcaacat ggtgagcaac acgttgcaga ccgcctcatg gttcaagggc
     6541 gccggagagg aggggaaggg cggcatgatg tgcgtcctcc cgttcttcca ctcctacggc
     6601 ctcggcgccg tgatgaacgt cgggatcgtg ctcggcctga agctcgtcct gctgccgagg
     6661 ttcgagctga agatggcgct caaggcgatc cagaaggaga agccgacgct gttcccaggc
     6721 gttccgcgga tctacatcgc gatcaacgag tcgccggaga cgacgaagtt cgatctcacc
     6781 tcgatcgtcg cgtgcttctc gggcgccgcg ccgctccccg tggcggtggc ggagaagttc
     6841 gagcagatca ccggcggccg cctcgtcgag ggctacgggc tgagcgagac ctcccccgtc
     6901 acgcacatca acccgatctt cggcaagcgc aagttcggct cgatcgggac cccgctgccg
     6961 gacaccgact gcaagctcgt cgatgtcgag gatccggacg tggaggtgtc gggcgatcag
     7021 gagggtgagc tctgcatctc gggcccacaa gtgatgaagg gctactggaa ccgccccgac
     7081 gagaccgccg gcatgatccg ccagcacgcc gacgggaagc tctggctgca cacgggtgac
     7141 atcgcgaaga tggacgagga gggctacttc tcgatcgtcg accggaagaa ggacatggtc
     7201 atcgtgtcgg ggttcaacgt ctacccgacc gaggtcgagc aggtcctgta ccggcacgag
     7261 aaggtgcaga aggtcgcggt cgtcggcgtt cccgacgaca cgaccggcga ggcggtgaag
     7321 gcctacgtcg tcctccgcga gggcgtcacg gcgaccccgg aagagatcat cgagtgggcg
     7381 cgcaaccccg agcacggcct cacgggctac cgggtcccga agcacgtcga gttccgcgac
     7441 gagctgccgg agacgatggt tgggaaggtg ctccgccgcg tcctgatcga cgaggagaag
     7501 cagaaggcgg cagcgagctc ctcctaggcg cgggaccgcc ggcgccggtg cgagccgcgg
     7561 tacttcggcc ggatgtcacg gtagagcagc acgaacgagc gccacgcgaa cagcagcgac
     7621 gcgagcccga gcgccgccag ggcggccatg agtggaacct cggtgatgcg ggtcacgtcg
     7681 gcgtgcccgc gtcgcgacgc cgctgatcgg cgcggcgctc cgcctcgagc cgctcgagac
     7741 cgaccgagga gtggtccacg aaccgcatcg cctccgcgat cgctcggtgt cccgcctgtt
     7801 cgccgatcag ccggtgcatc tcctgcgcga cgcgtcggta ggcgggatgc ccctggggcg
     7861 tagtgcggag ctcgatcaga tgcatcgcct cgcgcgcgtt catctgcatc acgaaacgga
     7921 tccggcacgc catcggcacg gcgtactggg ccgcgagccc gagccccgcg tcccgcaggg
     7981 tcttccacag gtccgcgcag gcggagagag aggcctcaac ctcctcgccg gcgcccactt
     8041 cctcgatctc gggggccaga tccgcgccgt ggccgggcgt cagctcctgc cactcgatcg
     8101 tgaggagccg gtgccgctgg aggtcgcgga acgctccgta gtcgcagagc acgtcgaacc
     8161 ggtacccggt gcgctcgaag gcgcgacccg gcttgtgccg ccggttcgac cgctctccga
     8221 cgtaggtccg cagcacgcgc atccgctcgc cgaccgtcat gtcgcgggcc cggcgcagga
     8281 gctggtcgtc gggcaggtcg gtgacctcgt acagggccgc cgcgacgacc ttgatctccc
     8341 cctgggggtc gaagtcggtc aggacgacct ccgggcgcgg ctcggctggc tcccgcacga
     8401 gctcttcggc ggccgccgtg agggatcgtc gggtttcgtc caggtaccgt gaccaccgcc
     8461 cgccccggtc gggcaggtcc acgcggcgga ggaaggccgg gatcaccttg cggagctcga
     8521 cgaggatcag gtcggcgtac gcccggacct cggccagcgg gtgcgcgcgc atccgcagga
     8581 ggagctgctc gtagctctgc ccggtcccga agatgccgac gttcgtgcgc gtcgcggccg
     8641 gcagcacccc gcggagcacg tcgcaggcct tcgctcggat cgtcgcgcga tagacggggt
     8701 ccggatcgga gggatcctgc gggaacagcc gccggaggtg gtcctgcatc ggctcgagcc
     8761 accgagcgta ggtctcgaag gcggcgtcga gcgtggtgcg gtaccgctcg gacagtcccg
     8821 cctcggcgac ctcggggggc tccgcgtacc gccaccggcc gcccgggcgg tcgtcgtagg
     8881 ggacgtagcg cgtcgactgc tcgaggtagg ccatgagccg cccgcgctcg aggaccttcg
     8941 ccaggatctg cgacgattgc tccgcggcca gatggacccc cccgagctgt gccacggagt
     9001 cgtcgccgta ctcgacgaag acccgctcgt agaggcgctc cgcgcgctcg acgccggccc
     9061 ccgcggacgt ggtctcctcc gtctcgtcgg cgaactcgtc caggaagagc cgtcgcaggg
     9121 acttcggcga gcgcgagtag cgcgcgaaca gagccccctt cgtgacctcg ggcaggttga
     9181 cgagcgcgaa gacgggtccg tcgacgttcg tgaagtgccg tgcgaggatc gtgcgctcct
     9241 cgggagtgaa cccctcctcg acgaaccaga gcgtcacgga gagggagacg ccgtggcggt
     9301 cggcgagggc gacgtcggcg gcggtcccgc cgacttccag acgcggacac ggctgccggc
     9361 ctgcacgctc tcacccgccc cggggtcttg gcggaagatg cgcccggcgg ggcacgcggt
     9421 cgtgacctcc tcgccgacga cgcgcatctg caggcccgca tcctcgagcc gtgcctgtgc
     9481 cgtcccctcg tcgacgcaat cgtccacgcg cgggaccacc accgtcgtga tcccgcggga
     9541 gaccacgacg ttcacctccc gccccgggcg gacctcgcgt cccgcatccg ggctctggtc
     9601 gagcacggtg tcaggcgcct cctcgctttc gacccgctcg accgagccca gtcgcagccc
     9661 cgcggcctgg aggtccgcgc gcgcgtcgtc gaggctcttc ccgatcaggt tcgggacctc
     9721 gaccggttcc ggcccggtcg agaccaggat cgtcacctcg ctgttctccg gtgccgacgt
     9781 ctcgggtttc gggttctggt cgaagacgcg gccctcctcg acctggtcgt gcggctcctc
     9841 gcggacccgc accgtgaagc ccgctcgttc gaggatccgc tcggcgcgtc cctggggctc
     9901 accgatgacg tcgggga
//
LOCUS       DATLGG010000244         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_61659, whole genome shotgun sequence.
ACCESSION   DATLGG010000244 DATLGG010000000
VERSION     DATLGG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61659"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1421
                     /locus_tag="VM638_07025"
     CDS             153..1421
                     /locus_tag="VM638_07025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVM12209.1"
                     /translation="MYLVENHTLPTVSMQLVFAGGSADDPKGKEGLASVCMGLLSDGT
                     EKLDKIAFSEALGDLASSIGSYAGVDEQAVTLSSLTKNLDPTLDLFVDTLLRPGMRAD
                     EFDRTIKKRLESLKQIKASPQGIAGRVAGPIAYGDDHPYGRVWTEASTKALEVEDCRA
                     YVKSWIQPANAKLFVVGDVTRKDVEEKVGKRLASWKGKAKTPVAIPAPKPRKGKVFLV
                     DVPGTPQALVRIMHGGPKRQDEDYVTNSLMVSILGGGFSSRINMNIREEKGWAYGAYG
                     GFSYARNFGLFSAGGSILVDKTADSIQEILREIERMKKEPVTEGELARERDGAILSLP
                     ASWATGAQVLSTYRSLVYYGLPLDWYSSYVPKVKAVTVGDVQAAAQEHLKQDVQILVV
                     GDAEKVAPELAKLGPILRLDADAKPTEQAR"
     gene            1418..2023
                     /locus_tag="VM638_07030"
     CDS             1418..2023
                     /locus_tag="VM638_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVM12210.1"
                     /translation="MMDARGALERLKEGNRRFASNVRSVESLLSQMRRDQLADGQKPF
                     AIVLGCSDSRAPAEIVFDQGLGDLFVIRVAGNVVAPSQIGSVEFAAERFGTRLVVVMG
                     HTSCGAISATLDHLTRPEEPLPGSLRSIVDRVKPALEPVVKGDTRDLVMEATRANVRH
                     SVEQIRRGSPILEGLIANAGLLVVGAVYDIRTGEVEFLDGA"
     gene            complement(2020..>2601)
                     /locus_tag="VM638_07035"
     CDS             complement(2020..>2601)
                     /locus_tag="VM638_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM12211.1"
                     /translation="GGMPWQPGVSITPLPEHGKPETLAAMVADAVSKGARVVNRDGGA
                     VHGTWMKPAVVSGVKPGMQLHELEQFGPVVPIATWRDEREVDEQMKASPYGQQMSIFG
                     KDPQRIARFIDAHVNGVSRINVNTQCRRGPDTFPFTGRKDSAEGTLSVSDALRAFSIR
                     CVVATSADPANTGLVTEIVTRRLSTFLSTDYVF"
BASE COUNT          402 a          891 c          939 g          369 t
ORIGIN      
        1 cgtgatctcc cccgcggccg gcggccgcgc cgacgccgca ggacctcgcc ttcccgcagg
       61 agccgttccg gcgccagccg ccggccgcgg gggagatcac gagcttcgag cagccgccgc
      121 tgacgcagtt cacgctcgcg aacggcatcc gggtgtacct cgtcgagaac cacaccctcc
      181 cgacggtgtc gatgcagctc gtgttcgccg gaggctcggc ggacgacccg aaggggaagg
      241 aggggctcgc ctccgtgtgc atggggctcc tctccgacgg gaccgagaag ctcgacaaga
      301 tcgccttcag cgaagccctc ggcgatctcg cctcgtcgat cggcagctac gccggcgtcg
      361 acgagcaggc ggtcacgctc tccagcctga cgaagaacct cgatccgacg ctcgacctgt
      421 tcgtcgacac cctcctccgc ccggggatgc gcgcggacga gttcgaccgg acgatcaaga
      481 agcggctcga gtcgctgaag cagatcaagg cgtccccgca ggggatcgcc ggccgcgtcg
      541 cgggcccgat cgcctacggg gacgatcacc cctacgggcg cgtctggacc gaggcgtcga
      601 cgaaggcgct cgaggtcgag gactgccgcg cctacgtgaa gtcgtggatc cagccggcga
      661 acgccaagct cttcgtggtc ggggacgtca cgcgcaagga cgtcgaggag aaggtgggga
      721 agcgcctcgc ctcctggaag gggaaggcga agacgccggt cgcgatcccc gcgccgaagc
      781 ctcgcaaggg gaaggtcttc ctcgtcgacg tgcccggcac gccgcaggcg ctcgtccgga
      841 tcatgcacgg cggcccgaag cgccaggacg aggactacgt gaccaactcg ctgatggtct
      901 cgatcctcgg cggcggcttc tccagccgga tcaacatgaa catccgcgag gagaagggct
      961 gggcctacgg cgcctacggc ggcttctcct acgcgcggaa cttcggcctc ttctccgccg
     1021 gcgggtcgat cctcgtcgac aagaccgccg actcgatcca ggagatcctc cgcgagatcg
     1081 agcggatgaa gaaggagccg gtgacggagg gggagctcgc gagggagcgg gacggcgcga
     1141 tcctgtcgct gccggcgagc tgggcgacgg gggcgcaggt cctctcgacc tatcggtcgc
     1201 tcgtctacta cgggctgccg ctggactggt actcgtccta cgtgccgaag gtgaaggcgg
     1261 tgaccgttgg cgacgtgcag gcggccgcgc aggagcacct gaagcaggac gtgcagatcc
     1321 tcgtcgtcgg cgacgcggag aaggtcgcgc cggagctcgc gaagctcggg ccgatcctcc
     1381 gcctcgacgc cgacgcgaag ccgacggagc aggcacgctg atggacgcgc ggggagccct
     1441 cgagcggctg aaggaaggga accggcggtt cgcctcgaac gtccggagcg tcgagtcgct
     1501 gctctcgcag atgcgccggg accagctcgc ggacgggcag aagccgttcg cgatcgtgct
     1561 cggctgctcg gactcgcgag cgccggccga gatcgtcttc gaccaggggc tgggcgacct
     1621 cttcgtcatt cgcgtggccg ggaacgtcgt cgccccctcg cagatcggga gcgtcgagtt
     1681 cgcggcggag cggttcggca cgcgcctcgt cgtcgtcatg gggcacacct cgtgcggcgc
     1741 gatcagcgcg acgctcgacc acctcacccg gccggaggag ccgctccccg gcagcctccg
     1801 ctcgatcgtc gatcgcgtga agccggcgct cgagccggtc gtcaagggcg acacgcggga
     1861 cctcgtgatg gaagcgacgc gcgcgaacgt gcggcactcc gtcgagcaga tccgccgcgg
     1921 ctcccccatc ctcgaagggc tcatcgcgaa cgcggggctc ctggtggtgg gcgcggtcta
     1981 cgacatccgc accggcgagg tcgagttcct cgacggcgcc tagaagacgt agtcggtcga
     2041 gaggaacgtg gacaggcgcc gggtgacgat ctccgtcacc aggccggtgt tcgccgggtc
     2101 ggcgctggtc gccaccacgc agcggatcga gaaggcgcgc agcgcgtcgg agacggagag
     2161 cgtcccctcc gcggagtcct tgcgcccggt gaaggggaag gtgtcggggc cgcggcggca
     2221 ctgcgtgttc acgttgatgc gggagacccc gttcacgtgg gcgtcgatga agcgggcgat
     2281 gcgctgcggg tccttgccga agatcgacat ctgctggccg tacggcgacg ccttcatctg
     2341 ctcgtcgacc tcccgctcgt cgcgccaggt cgcgatcggg acgacggggc cgaactgctc
     2401 gagctcgtgg agctgcatcc cgggcttcac gccggagacg accgcgggct tcatccacgt
     2461 cccgtggacc gcgccgccgt cgcggttcac gacgcgcgcg cccttcgaca cggcgtccgc
     2521 gaccatcgcc gcgagcgtct cgggctttcc gtgctcgggg agcggcgtga tcgagacgcc
     2581 gggctgccac ggcattccgc c
//
LOCUS       DATLGG010000245         3541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_61721, whole genome shotgun sequence.
ACCESSION   DATLGG010000245 DATLGG010000000
VERSION     DATLGG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61721"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1192)
                     /gene="sat"
                     /locus_tag="VM638_07040"
     CDS             complement(53..1192)
                     /gene="sat"
                     /locus_tag="VM638_07040"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003327643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HVM12212.1"
                     /translation="MTIDPHGGELLDLTDAEAAGEAQHLPKIPLGPREQADLEMLAVG
                     AMSPLGGFMGEKDYRSVLDEMRLASGLIWSLPITLSLTEDQVKQVGGADAVALTIGDR
                     PAAVLQVEEVYRRDREVEAERVYGTTDDAHPGVKAVADGGEWLVGGPVRVASLPDDRP
                     FPDRRLTPAQTRAAFEERGWRRVVGFQTRNPVHRAHEYIQKCALEICDGLLLHPLVGE
                     TKGDDIPADVRMRCYEVLLDGYYPKDRVLLSVFPAAMRYAGPKEAIFHALVRKNYGCT
                     HFIVGRDHAGVGTYYGTYDAQKIFERFEPEELGIEPLMFEHSFWCRRCEGMATTKICP
                     HGDDDRLSLSGTKVREMLRAGERPPPEFTRPEIADVLIEAMSAAD"
     gene            1301..2026
                     /locus_tag="VM638_07045"
     CDS             1301..2026
                     /locus_tag="VM638_07045"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HVM12213.1"
                     /translation="MQTLLAPELIDDLNQRFEQATAQEIVRWALEGSGIEKVALASSF
                     QDEAVALIHMAVQVRPDVPVIFLNTGFHFAETLAFKEQLVERFGLNLLELTGEETVES
                     QAAKHGPRLYERDPNLCCRINKVLPLDRTLRTLDAWMTGLRRDQAPTRAGTPIIDRYE
                     LEPGRWIAKISPLANWTKREVVRYLGEHDLPRHPLYRLGFASIGCAPCTRALFPGEDD
                     RAGRWPGADKVECGIHLKPPANG"
     gene            complement(2023..2682)
                     /locus_tag="VM638_07050"
     CDS             complement(2023..2682)
                     /locus_tag="VM638_07050"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HVM12214.1"
                     /translation="MARGGAGKALVIVFATLVVLGVAVDIVARLVVEGSLAERLRNEL
                     RLDEPPDVSLKGFPFLLHLARQRFPAATVDARNVDEAEVRVRRLLLEVEDLRFHGGGG
                     VVRAERGSAELEIDEEALTELARREGLPGRVELLGPEVRITLEEGVEASVTGTLALED
                     GEVVFEPEQAEPVDPPVPLTLRFPLPQIVEGIAFREVEVREGVAVIRAALDASILRVR
                     V"
     gene            complement(2686..3078)
                     /locus_tag="VM638_07055"
     CDS             complement(2686..3078)
                     /locus_tag="VM638_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004572502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HVM12215.1"
                     /translation="MPPTLTDRQRGFFERPNFGHLATLMPDGSPHVSPVWVDVDGDEI
                     LVNTAAGRLKERNVRRDPRVAISVRDQDLVEEEVVVRGRVVGITAEGADEHIDALSRK
                     YTGKPFGSRTPGAQRLILRIQPEHVAGG"
     gene            complement(3104..>3541)
                     /locus_tag="VM638_07060"
     CDS             complement(3104..>3541)
                     /locus_tag="VM638_07060"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVM12216.1"
                     /translation="GLVRVGYGEEALDDVARRLSPRILRVPRRVDPARRQLERYFAGR
                     LRRFDIDLDWSLTNGFSRRVLRAAARIPFGEVSTYREVAARAGSPRGSRAAGNALGSN
                     PIPIIVPCHRVLHTAGGLGGYTGGLDRKEFLLRLEGVLPPLPG"
BASE COUNT          517 a         1268 c         1270 g          486 t
ORIGIN      
        1 cttgcatggg cgccacccac taccgattgg gcctcggccc cggcccgaac gcctagtcgg
       61 cggcggacat cgcctcgatc aggacgtcgg cgatctcggg gcgggtgaac tcgggcggtg
      121 ggcgctcgcc ggcgcggagc atctcgcgga ccttggttcc ggagagcgag aggcggtcgt
      181 cgtcgccgtg ggggcagatc ttcgtcgtcg ccatgccctc gcagcgacgg caccagaagg
      241 agtgctcgaa catcagcggc tcgatgccga gctcctcggg ctcgaatcgc tcgaagatct
      301 tctgcgcgtc gtaggtgccg tagtacgtgc cgacgccggc gtggtcgcgg ccgacgatga
      361 agtgcgtgca gccgtagttc ttgcggacca gcgcgtggaa gatggcctcc ttcggcccgg
      421 cgtagcgcat cgcggcgggg aagacggaga gcaggacgcg gtccttcggg tagtagccat
      481 cgaggaggac ctcgtagcag cgcatccgga cgtcggcggg gatgtcgtcg cccttcgtct
      541 cgccgacgag cgggtggagc aggagcccgt cgcagatctc gagggcgcac ttctggatgt
      601 actcgtgcgc gcgatggacc gggttgcggg tctggaaccc gacgacgcgc cgccagccgc
      661 gctcctcgaa ggcggcacgg gtctgcgccg gcgtgaggcg ccggtccggg aacgggcgat
      721 cgtccggcag cgaggcgacg cgcaccgggc ccccgacgag ccactcgcca ccgtcggcca
      781 cggccttcac gcccgggtgg gcgtcgtcgg tggtgccgta gacgcgctcg gcctcgacct
      841 cgcggtcccg gcggtacacc tcctcgacct ggaggacggc cgccgggcga tcgccgatcg
      901 tcagcgcgac cgcgtcggcg ccgccgacct gcttcacctg atcctccgtc agcgacagcg
      961 tgatcggcag gctccagatc aacccgctcg cgaggcgcat ctcgtcgagg accgagcggt
     1021 aatccttctc gcccatgaag ccgccgagcg gggacatcgc accgacggcg agcatctcga
     1081 ggtccgcctg ctcgcgcgga ccgaggggga tcttcggcag gtgctgggcc tcgccggcag
     1141 cctcggcgtc ggtgaggtcc aacagctcac cgccgtgggg gtcgatcgtc atcgtcacac
     1201 ctcgcaggat cgggtcgggt cgggcccccg caggatagac gaccgggggc cgcgcgcggg
     1261 tccggcgcta tactcgacgt gacctcgcgg aaggaacctc gtgcagaccc tcctcgctcc
     1321 cgagctcatc gacgacctca accagcgctt cgaacaggcg acggcgcagg agatcgtccg
     1381 ctgggcgctg gagggatcgg gcatcgagaa ggtcgcgctc gcgagctcgt tccaggacga
     1441 ggcggtggcg ctgatccaca tggccgtcca ggtccggccg gacgttccgg tgatcttcct
     1501 gaacaccggg ttccatttcg cggagaccct ggcgttcaag gagcagctcg tcgagcggtt
     1561 cggtctcaac ctcctggagc tgacgggcga ggagacggtc gagtcgcagg ccgcgaagca
     1621 cggcccccgc ctctacgagc gcgacccgaa cctgtgctgc cggatcaaca aggtcctccc
     1681 gctcgaccgc acgctccgga cgctcgatgc ctggatgacc ggcctgcgcc gcgaccaggc
     1741 gccgacccgc gcgggcacgc cgatcatcga ccgctacgag ctcgagcccg ggcgctggat
     1801 cgcgaagatc agcccgctcg cgaactggac gaagcgggag gtcgtgcgct acctcggcga
     1861 gcacgatctg ccgcgacacc cgctctaccg cctcggcttc gcctcgatcg ggtgcgcccc
     1921 gtgcacccgg gccttgttcc cgggcgagga tgaccgcgcc ggccgctggc cgggggccga
     1981 caaggtcgag tgcggcatcc acctgaagcc gcccgcgaac ggctagacgc ggacgcgcag
     2041 gatcgaggcg tcgagcgccg cgcggatcac ggcaaccccc tcacggacct cgacctcgcg
     2101 gaacgcgatc ccctccacga tctgtgggag cgggaaacgc agcgtgaggg gaaccggcgg
     2161 gtccacgggc tcggcctgct ccggctcgaa gacgacctca ccgtcctcca gcgcgagggt
     2221 ccccgtcacc gacgcctcga cgccctcctc gagcgtgatc cgcacctcgg gcccgaggag
     2281 ctccacccgc ccggggagcc cttcgcggcg cgcgagctcg gtgagcgcct cttcgtcgat
     2341 ctcgagctcg gccgagccgc gctccgcgcg gacgacgcct cccccgccgt ggaaccgcag
     2401 gtcctcgacc tcgaggagga gccggcgcac ccgaacctct gcctcgtcca cgttccgtgc
     2461 gtccaccgtt gcggcgggga aacgctgacg cgcgaggtgg agcaggaagg ggaacccctt
     2521 gagcgagacg tccgggggct cgtcgagacg gagctcgttg cgcagccgct ccgcgaggga
     2581 cccctcgacg acgagccgcg cgacgatatc gaccgccacg ccgaggacga cgagggtcgc
     2641 gaagacgatg acgagggcct tgccggctcc cccgcgtgcc acggttcagc cgcccgcgac
     2701 gtgctcgggc tggatccgaa ggatcaagcg ctgggcgccc ggcgtccggc tgccgaaggg
     2761 cttgcccgtg tacttccggg agagcgcgtc gatgtgctcg tcggccccct ccgccgtgat
     2821 gccgacgacc cggccgcgca cgaccacctc ctcttccacc aggtcctggt cgcgcacgga
     2881 gatcgcgacc cgcgggtcgc gccgcacgtt gcgttccttg aggcgcccag ccgccgtgtt
     2941 cacgaggatc tcgtcgccgt cgacgtccac ccacacgggc gagacgtgcg gcgagccgtc
     3001 cggcatgagg gtggcgaggt ggccgaagtt cggtcgctcg aagaagccgc gctgtcggtc
     3061 ggtcagggtc gggggcatcg tcacctccag gtcggcgcca accttacccc ggcagcggcg
     3121 gcaggacgcc ctcgagccgg aggaggaact ccttgcggtc cagtcccccg gtgtagccgc
     3181 cgaggccgcc tgccgtgtgc aggacccggt ggcagggcac gatgatcggg atggggttcg
     3241 acccgagcgc gttgccggcg gcgcgcgacc ctcgcggact gccggcgcgt gcggcgacct
     3301 cccgataggt gctgacctcg ccgaagggga tccgcgccgc ggcgcgcagg acgcgtcgcg
     3361 agaagccgtt cgtgagtgac cagtcgagat cgatgtcgaa gcgccgcaga cgtccggcga
     3421 agtagcgctc gagctgccgg cgggccgggt cgacgcgccg gggcacccgc aggatgcgcg
     3481 gcgacaggcg gcgggcgacg tcgtccagcg cctcctcgcc gtatcccacg cgcaccagcc
     3541 c
//
LOCUS       DATLGG010000246         4819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_8333, whole genome shotgun sequence.
ACCESSION   DATLGG010000246 DATLGG010000000
VERSION     DATLGG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8333"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..453
                     /locus_tag="VM638_07065"
     CDS             181..453
                     /locus_tag="VM638_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HVM12217.1"
                     /translation="MPSIGFGEILVILVFALVIFGPRRLPEIGRTIGKSMRDFRRAGA
                     EIRAELELDAEPPVVPNPNRVERKLDEKEPPPGPQDERAPEGGGSS"
     gene            456..1229
                     /gene="tatC"
                     /locus_tag="VM638_07070"
     CDS             456..1229
                     /gene="tatC"
                     /locus_tag="VM638_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVM12218.1"
                     /translation="MPLRRRRRDPEATMTVVEHLDELRKRLIISLGAVALCSVAGFAF
                     YGRILGFLTAPYCRALAALPASARPAGTLAGCRLVYSSPVDPFLTVLKVGFFSGLLLA
                     LPVVLWQVWRFVTPALTKRQRRLGIAFVVVSVLLFAGGAIFSVAVVPRGLTFLFRFGE
                     ESLTPLLTADRYLGFLMFLILAFGLSFELPLAMVFLAGARVVTSTQMRRARRFVYLGL
                     AVFAAILTPTQDPYTMLVMLVPLIGFYEVAILVARAFKR"
     gene            1250..2086
                     /locus_tag="VM638_07075"
     CDS             1250..2086
                     /locus_tag="VM638_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12219.1"
                     /translation="MAIKGKRKGGGGRRVASPPRPVMIPPRTPLLRRRSTRILIAVVL
                     LGAAAAITVGVMAARNTSRRLEAARSDVRQFQTRVEAALTADGIGQDTGTGFVILPQL
                     GQTLAQVQNDEEVDPEVEGQIEDWAEGTVKAADTIAGLDVGTSELSAEALRESATMMR
                     QGLMLYSTLARNLGLSMDLSGRQKDRLVAQLQEQFAATSVVFDTGWQKLQALRAELGI
                     PEPVPQQVPGMPGQVPGLPGGQVPGFPGGELPPGFDPNQIPVPEGEAPEGEAPAGEEA
                     EG"
     gene            2083..2811
                     /locus_tag="VM638_07080"
     CDS             2083..2811
                     /locus_tag="VM638_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVM12220.1"
                     /translation="MKPLALIHAFPLDASMWDEQVAALAGDTRVLAPSLPGFGGVPSA
                     GDVLTMERMADHVVAELDRQEVERAVIGGLSMGGYAALELWRRHRDRVAALILADTRA
                     EPDDDAGREKRRALAEKVRAEGSGAVADAPPPLLAEGAEPDLWEAVKATIRRQPAEAI
                     AAAALGMAERPDSGPTLATIDVPTLVIVGSEDTLTPPPLSERLAREIPGAQLVTLEGA
                     GHLSNLEDPDGFTKAVRGFLERVG"
     gene            2841..>4819
                     /locus_tag="VM638_07085"
     CDS             2841..>4819
                     /locus_tag="VM638_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVM12221.1"
                     /translation="MDPSSFMQRYPFDLDRFQVEAIESLARGESVLVAAPTGSGKTVV
                     AEFAAERAISTGRKAFYTTPLKALSNQKFGDFIARHGAANVGLLTGDNTVNGEAPIVV
                     MTTEVLRNMLYERSATLEGLDVVIMDEVHYLQDPYRGAVWEEVLIHLAPTVSVVCLSA
                     TVSNAEEFGEWLATLRGTTRVVIEERRPVALENLYMTGRDLHPMHIEQEGRLLPNPYL
                     VSLTQREVRAREYRRSSDGRRQYERRSRPREGHRRVYVPRREEVVLRLDEEGMLPAIY
                     FVFSRAGCDKSVGFLMESGVRLTTREEEARIREFAEMRAAWIDEEDLATLGFFEMREA
                     LAAGIAAHHAGMLPVFKETVEELFAAGLVRVVFATETLSLGINMPARTVVIEDLWKFS
                     GERHELLTPGEYTQLTGRAGRRGIDEVGFAVVLHQKQVDFERAAGLATTRTYELTSSF
                     RPSYNMAVNLVRNYTSEQAHHLLNSSFGQFQADRGVVALQRQLDRDSAALEGYLERTR
                     CELGDFAEYWSLREEAEQIRREAAREVDREQQHEVRAAIDALRPGDVVHVPRASRRGL
                     AVVLSNRDGRPTMLTQDRRFFRASPKDFPDPPTKLARVPLPRGSARSARYRRDLASQL
                     ASLNVRPPKVRRRRVSGAELARAEELDERAERHPVT"
BASE COUNT          668 a         1696 c         1771 g          684 t
ORIGIN      
        1 atcctgacca tcctcgtcgt ctcgctcgcc cgccgcgtcg gccgtctcgc tcgagcgatg
       61 caggcgttcg aggctgatgc ccggcccatc gtcgagcggc tgcgcaccgg atccgaggcc
      121 gcgaagcggc gcatgcagga gctgtcggaa cgcgccacgc cgccggagac cccgtaggcg
      181 atgcccagca tcgggttcgg ggagatcctc gtgatcctcg tcttcgccct cgtcatcttc
      241 ggaccacgcc gccttccgga gatcggaagg acgatcggca agtcgatgcg ggacttccgc
      301 cgggcgggag ccgagatccg cgcggagctc gagctggacg cggagccgcc cgtcgtgccg
      361 aaccccaacc gggtcgagcg gaagctcgac gagaaggagc ccccgccggg tccgcaggac
      421 gagcgagccc ctgagggcgg cggcagctcc tgacgatgcc gctccgccgg cgccgccgag
      481 accccgaggc gacgatgacc gtcgtcgagc acctcgacga gctgcggaag cgcctgatca
      541 tctcgctcgg ggccgtcgcg ctctgctcgg tggccgggtt cgcgttctac ggacggatcc
      601 ttggcttcct gaccgcgccc tactgccggg cgctcgccgc gctgccggcc tccgcgcgtc
      661 cggccggaac gctcgcggga tgtcgcctcg tctacagcag ccccgtcgat cccttcctga
      721 cggtcctgaa ggtcgggttc ttctcggggc tgctcctggc tctcccggtc gtcctgtggc
      781 aggtctggcg gttcgtgacg cccgccctga cgaagcggca gcgacgactg ggcatcgcct
      841 tcgtcgtcgt ctcggtcctg ctgttcgcgg ggggcgcgat cttctccgtc gccgtggtcc
      901 cgcgcgggct gaccttcctg ttccggttcg gggaggagag cctcacgccg ctgctgacgg
      961 cggaccggta cctcgggttc ctgatgttcc tgatcctggc gttcggcctg tcgttcgagc
     1021 tcccgctcgc gatggtgttc ctcgccggcg cacgcgtggt cacgagcacc cagatgcggc
     1081 gggcgcggcg gttcgtgtac ctcgggctgg ccgtgttcgc ggcgatcctg accccgaccc
     1141 aggacccgta cacgatgctg gtgatgctcg tgcccctgat aggcttctac gaggtcgcga
     1201 tcctcgtggc ccgcgcgttc aaacgatgac cgaacggagg taggacctcg tggcgatcaa
     1261 ggggaagcgg aagggcggcg gcggtcgtcg ggtggcgagc ccgccgcggc cggtcatgat
     1321 cccgccgcgg acgccgctgc tgcggcgccg gagcacccgg atcttgatcg cggtcgtgct
     1381 cctcggtgcg gccgccgcga tcacggtcgg ggtgatggcg gcgcggaaca cgagccgccg
     1441 gctcgaggcg gcgcgcagcg acgtgcggca gttccagacc cgcgtcgagg cggccttgac
     1501 ggccgacggg atcgggcagg acaccggaac ggggttcgtg atcctcccgc agctgggcca
     1561 gaccctggcc caggtccaga acgacgagga ggtcgacccc gaggtcgagg gccagatcga
     1621 ggactgggcg gaggggaccg tgaaggccgc ggatacgatc gccggcctcg acgtcggcac
     1681 gagcgagctc tcggccgagg ccttgcgcga gtccgccacg atgatgcgcc agggcctgat
     1741 gctgtactcg acgcttgccc ggaacctcgg tctctccatg gacctgtcgg gccggcagaa
     1801 ggaccggctg gtcgcccagc tccaggagca gttcgccgcc acctccgtcg tcttcgacac
     1861 cggctggcag aagctccagg cgctccgggc ggagctcggg atccccgagc cggtcccgca
     1921 gcaggtgccc gggatgccgg ggcaggtgcc cggtctgccg ggtggacagg tcccgggctt
     1981 ccccggcggc gagctcccgc ccggcttcga cccgaaccag atccccgtcc cggagggaga
     2041 agcgcccgag ggcgaagccc ccgccggaga ggaggccgaa ggatgaagcc gctcgcgctg
     2101 atccacgcat tccccctcga cgcctcgatg tgggacgagc aggtcgcggc cctggccggg
     2161 gacacgcggg tcctcgcccc gtccctgccg ggcttcgggg gcgtcccctc cgcgggggac
     2221 gtcctgacga tggagcggat ggccgatcac gtggtcgccg agctcgaccg tcaggaggtc
     2281 gagcgggcgg tcatcggcgg cctctcgatg ggcgggtacg ccgccctgga gctctggcgg
     2341 cgccaccgag accgggtcgc ggcgctgatc ctggccgaca cccgcgccga gcccgacgac
     2401 gacgccggcc gcgagaagcg acgcgcgctc gccgagaagg tgcgggccga gggcagcgga
     2461 gccgtcgcgg acgcgccccc accgctcctg gccgagggcg cggagccgga tctgtgggag
     2521 gccgtcaagg cgacgatccg acggcagccc gcggaggcga tcgccgcagc ggccctcggg
     2581 atggccgagc gccccgactc ggggccgacg ctggcgacga tcgacgtccc gaccctcgtg
     2641 atcgtcgggt cggaggacac gctgacgccg ccgcccctct cggagcggct cgcgcgggag
     2701 atccccgggg cgcagctcgt gaccctggag ggcgcgggcc acctctcgaa cctcgaggac
     2761 ccggacgggt tcacgaaggc cgtgcggggt ttcctcgagc gcgtcggctg agcccgcacg
     2821 gtcggggtag cgtggaccgc gtggacccca gctcgttcat gcagcgctac cccttcgacc
     2881 tggaccgctt ccaggtcgag gcgatcgagt cactcgcgcg cggcgagtcc gtcctcgtgg
     2941 ccgcgccgac cgggtcgggg aagacggtcg tcgcggagtt cgccgccgaa cgagcgatct
     3001 cgaccggacg gaaggccttc tacacgacgc cgctgaaggc cctgtcgaac cagaagttcg
     3061 gcgacttcat cgcgcgacac ggcgccgcga acgtgggcct gctgacgggc gacaacaccg
     3121 tgaacgggga ggcgccgatc gtcgtgatga cgacggaggt cctgcggaac atgctctacg
     3181 agcgcagcgc gacgctcgag gggctcgacg tcgtgatcat ggacgaggtg cactacctcc
     3241 aggaccccta ccgcggagcc gtctgggagg aggtcctgat ccacctcgcg ccgacggtct
     3301 cggtcgtgtg cctctcggcc accgtgtcga acgcggagga gttcggggag tggctcgcga
     3361 cgctccgcgg aacgacgcgc gtcgtgatcg aggagcgccg cccggtcgcg ctcgagaacc
     3421 tctacatgac ggggcgtgac ctccacccga tgcacatcga gcaggaggga cgtctcctgc
     3481 cgaaccccta cctcgtctcc ctcacccagc gtgaggtccg ggcgcgcgag taccgccgga
     3541 gctccgacgg gcggcgtcag tacgaacgcc gctcgcggcc gcgtgagggg caccggcgcg
     3601 tctacgtccc acggcgcgag gaggtcgtcc tgcggctgga cgaggaaggg atgcttccgg
     3661 cgatctactt cgtcttctcg cgggcggggt gcgacaagag cgtggggttc ctgatggagt
     3721 cgggggttcg cctgacgacg cgggaggagg aggcgcgcat ccgcgagttc gcggagatgc
     3781 gcgcggcctg gatcgacgag gaggatctgg cgacgctcgg gttcttcgag atgcgcgagg
     3841 cgctcgcggc cggcatcgcc gcgcaccacg ccgggatgct gccggtgttc aaggagaccg
     3901 tcgaggagct gttcgccgcc ggcctggtgc gggtggtgtt cgcgacggag accctgtcgc
     3961 tggggatcaa catgccggct cgaaccgtcg tcatcgagga cctgtggaag ttctccggcg
     4021 agcgacacga gctcctgacg cccggggagt acacccagct caccgggcga gcggggcgcc
     4081 ggggcatcga cgaggtcggc ttcgccgtgg tcctccacca gaagcaggtg gacttcgagc
     4141 gagcggcggg cctcgccacc acgcggacct atgagctcac ctcctcgttc cggccgtcct
     4201 acaacatggc ggtgaacctc gtccgcaact acacgtcgga gcaggcgcac cacctgctca
     4261 acagctcgtt cggccagttc caggccgatc gcggggtcgt cgccctccag cgccagctcg
     4321 accgcgactc ggcggccctc gaggggtacc tcgagcgcac gcgctgtgag ctcggggact
     4381 tcgccgagta ctggtcgctc cgggaggagg ccgagcagat ccggcgggag gcggcacgcg
     4441 aggtcgaccg ggagcagcag catgaggtcc gcgccgcgat cgacgccctg cgcccgggcg
     4501 acgtcgtcca cgtgccgcgt gccagccggc gcggactcgc ggtggtgcta tcgaaccgcg
     4561 acggacgccc gacgatgctg acccaggacc ggcgcttctt ccgggcatcg ccgaaggact
     4621 tccccgaccc cccgacgaag ctcgcccgcg tcccgctccc gcgggggagc gcgcgcagcg
     4681 cccggtaccg ccgcgacctg gcgtcgcagc tcgcctcgct gaacgttcgc ccgccgaagg
     4741 tccggcggcg gcgcgtctcg ggggccgagc tcgcgcgggc ggaggagctg gacgagcgag
     4801 ccgagcggca tccggtcac
//
LOCUS       DATLGG010000247         4236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_120453, whole genome shotgun sequence.
ACCESSION   DATLGG010000247 DATLGG010000000
VERSION     DATLGG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120453"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1033
                     /locus_tag="VM638_07090"
     CDS             137..1033
                     /locus_tag="VM638_07090"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HVM12222.1"
                     /translation="MSETWKETLAVEAHEAMDRLATWLPERWGSALFRAAGTAALRLA
                     PGARRTVQENLARVLGEAPGSAIVERTAREAFRSYARYWHETFLIRELPRERFLERFT
                     VIGREHLDAAHDRGRGGLLALPHLGNWDAAGRWVAELGYRITAVAEELRPPRMYRLFH
                     RHRRRLGMEIVPLSDARNVGQELASRLARNHFIALVCDRDLKGTGVEVEMFGERRKMP
                     AGPALLSLVSGSPLFPCATFDTEDGWAAVIHPPLEIERTGSLRADVAELTRELARRFE
                     REISAHPVQWHMFQPAWNGSPR"
     gene            1030..2130
                     /locus_tag="VM638_07095"
     CDS             1030..2130
                     /locus_tag="VM638_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVM12223.1"
                     /translation="MRVALVCPYAWDAPGGVQVHVRQLAERLRGLGHATLVVAPADGR
                     RAEPGVVRTGGPIRVRVNRSVAPIAPWPAAARRRRDALGAFRPDIVHVHEPFVPGAAG
                     AVRDGARLGAATVGTFHAYADRAPLLDVTVPLLRSTWSALDRRIAVSRAAAAFVARRF
                     RGGVRVVPNGVETRLFAEADPVDLPPGRRVLFVNRLDPRKGFGVMVRAFEDLRRTHPD
                     ALLVVAGDGPERGAVGSLEPETRARVVLLGNVAHRDLPRVHAACEIFCAPAVGHESFG
                     IVLVEAMAAGLPVVASDIPGYREVVRDGVDGVLVPPRAPAATAAALRRLLDDPAVADG
                     FRAAGRQRAGRFGWETVAAEILGIYEEALRER"
     gene            2120..3043
                     /locus_tag="VM638_07100"
     CDS             2120..3043
                     /locus_tag="VM638_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVM12224.1"
                     /translation="MSDDAFALRLARAVADRIRPLMGTADARRRVGTAHGGDPTYAID
                     AEAETLVREAFAERSDVAYFTEDEGLVVRGSPRRLFLVDPIDGTRPAAAGFETCCVSI
                     AVAPFGAEPALGDVEYGCLVEIATGSVLEARRGAGASGDRAVEPSHARAVRGAFWAGG
                     FRGQPAVLTATVLEEIFDAPGSEGAYFDQGSAAYSLACVATGRLDAYVDVGAAIVDEI
                     PGARAAFEALGDGSILNSVTYDVAAGALLAREAGCAISDPRGRSLAEVPLLAADGSAS
                     PVATVCAGTPELHAELLAAVDRGLERARSLS"
     gene            complement(3036..>4236)
                     /gene="fahA"
                     /locus_tag="VM638_07105"
     CDS             complement(3036..>4236)
                     /gene="fahA"
                     /locus_tag="VM638_07105"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HVM12225.1"
                     /translation="FPIQNLPFGIVRPRDSAPRVATRIGDHVLDLAAVHRVGLLPEVP
                     PEVVEARNLNPLLGRDRATWRALRHRLSVLLSEGNGEIAAAGDAGDALLPIDEVELLL
                     PVEVGDYVDFYSSLEHATNLGRMFRPEGDPLLPNWRHLPIGYHGRGGTVVVSGTPIRR
                     PWGQVTPGEGPPSFRPTQRLDIELEVGFVTGGGNPLGTPIPVGAAADHIFGLVLVNDW
                     SARDIQRFEYQPLGPFLGKSFATTISPWVVTLDALEPHRVPGPPQEPEPLPHLRRAED
                     RAYAIDLEVLLAAGGGDPTPISRTTFAGMYWTIAQQLAHATSNGATARPGDLFASGTI
                     SGADPGTEGSLIELTRDGPIRLPDGSERRFLEDGDTVILHGWAGGDGRPRIGFGECRG
                     TVLPALS"
BASE COUNT          498 a         1520 c         1632 g          586 t
ORIGIN      
        1 gttcggtcct cctcggcgcg ccggcggtcg gcctcgtcgg cggcctcgtg gagcctgggc
       61 tctgggtggc ggtcgcgtgg tcgagcatcg tcgcggtgaa gcgagcgttc gaggccgcaa
      121 cgcaggggga acccggatga gcgagacgtg gaaggagacg cttgccgtcg aagcccacga
      181 ggcgatggac cgcctcgcga cctggctgcc ggagcgatgg ggcagcgcgc tcttccgcgc
      241 ggccggcacg gcggcgttac gtctggctcc gggtgcccgt cgcaccgtgc aggagaacct
      301 cgcacgcgtc cttggtgagg cgccgggctc cgccatcgtc gagcggaccg cgcgcgaggc
      361 gttccgatcg tacgcacgct actggcacga gaccttcctg atccgagagc tgccgcggga
      421 gcgattcctc gagcggttca cggtcatcgg ccgcgagcac ctcgatgccg cgcacgatcg
      481 cggccggggc gggttgctgg ccctgccgca cctggggaac tgggacgcgg ccgggcgttg
      541 ggtcgccgag ctcggctatc gcatcacggc ggtcgcggag gagctccggc ctccccggat
      601 gtaccgcctc ttccatcggc atcggcgccg cctcgggatg gagatcgtgc cgttgtccga
      661 cgcgcgcaac gtcggccagg agctcgcgtc gcgtctcgcc cgtaaccact tcatcgcgct
      721 cgtctgcgat cgcgatctca aggggaccgg cgtcgaagtg gagatgttcg gcgagcggcg
      781 gaagatgccg gcgggtccgg cgctgctctc cctcgtctcc gggagtccgc tcttcccctg
      841 tgcgaccttc gacaccgagg acggctgggc ggcggtgatc cacccgccgc tggagatcga
      901 gcggacgggg agcctgcgcg ccgacgtcgc cgagctcacc cgtgagctgg ctcgccggtt
      961 cgagcgcgag atcagcgccc accccgtgca gtggcacatg ttccagcccg cctggaacgg
     1021 ctcacctcga tgagggtcgc cctcgtctgc ccctacgcgt gggatgcccc cggcggtgtg
     1081 caggtgcacg tccgccagct cgccgagcgg cttcgagggc tcggccacgc cacgctggtc
     1141 gtcgctccgg ccgatggccg gcgcgctgag ccaggggtgg tgcggacggg gggtccgatc
     1201 cgggtccggg tcaaccggtc ggtcgcgccg atcgcgccgt ggccggcggc ggcgcgccgg
     1261 cgtcgggacg ccctcggtgc gttccgtccc gacatcgtcc acgtccacga gccgttcgtg
     1321 ccgggcgccg ccggggcggt ccgcgacggg gcgcggctcg gcgccgccac cgtcggcacc
     1381 ttccacgcct acgcggaccg cgcgcccctc ctcgacgtga cggtcccgct gctgcgctcg
     1441 acctggtcgg cgctcgaccg gcggatcgcg gtgtcacggg cagcggccgc gttcgtcgct
     1501 cgccggttcc gcggcggggt gcgcgtcgtg ccgaacggcg tcgagaccag gctcttcgcg
     1561 gaggcggatc cggtcgacct gccgcccggg cgccgggtgc tcttcgtgaa ccggctcgac
     1621 ccgcggaagg ggttcggggt gatggtgcgc gcgttcgagg acctccggcg gacgcatccg
     1681 gatgcgctgc tcgtcgtggc gggcgatggt ccggagcgcg gcgccgtcgg gtccctcgag
     1741 ccggagacgc gggcccgcgt cgtcctcctc ggcaacgtcg cgcaccggga cctcccgcgg
     1801 gtccacgcgg cgtgcgagat cttctgcgct ccggcggtcg gccacgagag cttcgggatc
     1861 gtgctggtcg aggcgatggc ggcgggcctg ccggtggtcg cctcggacat ccccgggtat
     1921 cgcgaggtgg tccgggacgg ggtggacggg gtgctcgtcc cgccgcgtgc cccggcggcg
     1981 accgccgcgg cgctccgccg cctgctcgac gaccccgccg tggccgacgg gttccgcgcg
     2041 gcgggccggc agcgcgccgg acgcttcggc tgggagacgg tcgcggcgga gatcctcggc
     2101 atctacgagg aggcgctgcg tgagcgatga tgcgttcgcg ctgcggctcg cacgcgccgt
     2161 cgccgaccgc atccggccgc tgatgggaac ggccgacgcg cgccgccggg tggggaccgc
     2221 gcacggcggg gatccgacct acgcgatcga cgcggaggcc gagacgctcg tccgggaggc
     2281 cttcgccgag cgctccgacg tcgcgtactt caccgaagac gagggactcg tcgtgcgcgg
     2341 atccccccgc cgtctcttcc tcgtcgaccc gatcgacggg acgcgtcccg cagcggcggg
     2401 gttcgagacc tgctgcgtct cgatcgcggt cgcgccgttc ggcgccgagc ccgcgctcgg
     2461 cgatgtcgag tacggctgcc tcgtggagat cgcgacgggg tccgtcctcg aggcccgtcg
     2521 aggcgcgggc gcgagcgggg accgcgcggt cgaaccgtcc cacgcacggg cggtgcgggg
     2581 cgcgttctgg gccggcgggt tccgcggtca gcctgcggtc ctgaccgcga ccgtgctcga
     2641 ggagatcttc gacgcgcccg ggtccgaggg ggcctacttc gaccagggga gcgcggccta
     2701 ctcgctcgcg tgcgtcgcga cgggccgcct cgacgcgtac gtggacgtgg gcgcggcgat
     2761 cgtggacgag atcccaggcg cgcgcgccgc gttcgaggcg ctcggagatg ggtcgatcct
     2821 caactcggtg acctacgacg tcgcggcggg ggccctcctc gcgcgcgagg ccggatgcgc
     2881 gatcagcgat ccgcgggggc ggagcctcgc ggaggtcccc ctgctcgcgg cggatgggag
     2941 cgcctcaccc gtggccaccg tgtgcgccgg cacgcccgag ctccacgccg agctcctcgc
     3001 ggcggtcgac cgcgggctcg agcgcgcgcg ctccctcagc tgagggcggg caggaccgtc
     3061 ccccggcact cgccgaaccc gatcctcggg cggccgtcgc cacccgccca gccgtgcagg
     3121 atgacggtgt caccgtcctc gaggaaccgc cgctcggacc catccggcag ccggatcggc
     3181 ccgtcgcgcg tcagctcgat caggctgccc tcggtgcccg ggtcggcgcc cgagatcgtc
     3241 ccggaggcga acaggtcccc ggggcgcgcg gtcgcgccgt tcgaggtcgc gtgcgcgagc
     3301 tgctgcgcga tcgtccagta catcccggcg aaggtcgtcc gggagatcgg cgtcgggtcg
     3361 cccccgccgg ctgcgagcag cacctcgagg tcgatcgcgt aggcgcggtc ctcggcccga
     3421 cggaggtggg ggagcggctc cggctcctgg ggcggaccag ggacgcggtg gggctcgagc
     3481 gcgtcgagcg tcaccaccca cggcgagatc gtggtcgcga aggacttgcc gaggaacggc
     3541 ccgaggggct gatactcgaa ccgctggatg tcgcgggcgc tccagtcgtt gacgagcacg
     3601 agcccgaaga tgtgatcggc cgccgccccg acggggatcg gcgtcccgag cggattgccg
     3661 ccgccggtga cgaagccgac ctccagctcg atgtcgaggc gctgcgtcgg ccggaagctc
     3721 ggaggcccct cgcccggcgt gacctgtccc cacggacgac ggatcggggt tcccgacacg
     3781 acgacggtac cgccccgccc gtggtagccg atcggcaggt gccgccagtt cgggagcagc
     3841 gggtccccct ccgggcggaa catccgcccg aggttcgtcg cgtgctcgag cgaggagtag
     3901 aagtcgacgt agtccccgac ctcgacgggc aggagcagct cgacctcatc gatcgggagg
     3961 agcgcgtcgc cggcgtcacc ggccgccgcg atctccccgt tcccctcgga caggaggacc
     4021 gagaggcggt gccgcagggc tcgccaggtc gcgcggtcac ggccgaggag cgggttgagg
     4081 ttccgcgctt ccacgacctc cggcgggacc tccggaagca gcccgacccg atggacggcg
     4141 gcgaggtcga ggacgtggtc gccgatccgc gtcgcgaccc gcggcgccga gtcgcggggc
     4201 cgaacgatcc cgaacgggag gttctggatc gggaag
//
LOCUS       DATLGG010000248         4360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_231390, whole genome shotgun sequence.
ACCESSION   DATLGG010000248 DATLGG010000000
VERSION     DATLGG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231390"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VM638_07110"
     CDS             complement(<1..192)
                     /locus_tag="VM638_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018649267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVM12226.1"
                     /translation="MIAFVEGTVAERSGDRVVVAAGGLGYEVLVPSGTLAALPPLGGT
                     VRLLTHLHVREDGMTLYGFS"
     gene            complement(189..692)
                     /locus_tag="VM638_07115"
     CDS             complement(189..692)
                     /locus_tag="VM638_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVM12227.1"
                     /translation="MFDRVVIGVDPGVAATGVAVVGTRGGDRPSVLEARTIRTPAGAP
                     EASRLRALAEELRSTLRAHAPSEMALERLAWGRNVGSAMSVSRASGVLLLVAAERGLE
                     IREYGPLEVKLAVTGNGTARKEDVRRMLGRLLRIDGVPSEPDAADAVAVAVCHLHQAR
                     VPTGSRR"
     gene            complement(747..1496)
                     /locus_tag="VM638_07120"
     CDS             complement(747..1496)
                     /locus_tag="VM638_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVM12228.1"
                     /translation="MAGHSKWAGIKHKKAVLDQRRGKLFAKLLRAIEVAAREGGGNLD
                     GNATLASAVQKAKDYSVPMDNIERAIKRGSGEMAGTRYDPVVYEGYAPGGVAILVEAL
                     TDNRNRTGQEVRHTFAKMGGNLGDPGSVAWQFERKGTLLVDVDAAPEEERLLELVMEA
                     GGEDLAREGDQWRITTAPTELQAMRAVLDAGGVPVASAELTMNPSTTVPVAGAEAKRV
                     LSLIDALDELDDVQGVHANFDIPDEVLAEVS"
     gene            complement(1496..2062)
                     /gene="pdxT"
                     /locus_tag="VM638_07125"
     CDS             complement(1496..2062)
                     /gene="pdxT"
                     /locus_tag="VM638_07125"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HVM12229.1"
                     /translation="MAGVKAGVLALQGDFREHARVLERLGATPLLVRTPADLAAVGCL
                     VIPGGESTTISRLARSRGLVEPIRDRAGAGMPVLGTCAGMIVMAAEVAGGIPLLELMD
                     IRVARNAYGRQTESFETDLEVDQVGPVRGVFIRAPVVERVGPAVTVLGEVGGTPVVVE
                     QGSLLAAAFHPELVGDGSLHRRMLERAG"
     gene            2165..>4360
                     /locus_tag="VM638_07130"
     CDS             2165..>4360
                     /locus_tag="VM638_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12230.1"
                     /translation="MDSGVRILPYLTTTGFAALAVAAAASWVHRREPLRGGFARAAGL
                     LAAVTAVERIHQATAYRSTALEDASIVLFLASGYALLSLSQRVVPIRRSVLVLLRALV
                     VTVAFVGVAAEMPTKPDAPMSAFQTGLASCIVAVWAACVLLPIVRFWQASRGRPPVQR
                     ARLRSLALGYGGIVLVLLTAFFAHSYASHPAFVGGVHLAVLGLIPLLYAAFVPPRWLR
                     RLWRATEEAATGEAMIQLLDPSTAADDVAASAVRWGARLVGGDAAFLTRDGQVQAAIG
                     LSIEDARRHAADLADVRQATVVQIDAPEPRHAVHLPVRDAGGEGALTVLSGRLTPLFG
                     VDEIAALNRFAASIWAAIQHASLVEALREQTARAEAMVQAVGDLGTGLVVVEDARFVD
                     ANEAYLRMSGYTLEELRALPSVLSIADPADLAELQERLARRAAGAPIGDHYETALIRK
                     DGRRVEIEVSIKDFERRGRLITLVRDITARKQAERTLRDGYEREREAVARLRTLDEMK
                     STFLAAVSHELRTPLTAISGFARTLDGRMEEFPATERDEMVRAIARNAVRLERLLSDL
                     LDLDRLQRGLLEPQGRPTDVGGLVRRTLVESELLEGREVSVDAGEVFAVVDPPKVERI
                     VENLVGNAVRHTPADTPIWVRVSATERDVLIVVEDAGPGVPAELRTAIFRPFEQGDRG
                     DGHSPGVGIGLSIVGRFAELHGGRAWVEERPGGGASFRVTLPLDGSERER"
BASE COUNT          556 a         1626 c         1561 g          617 t
ORIGIN      
        1 ggagaacccg tagagggtca tcccgtcctc gcgcacgtgc aggtgggtga ggagacggac
       61 cgtgccgccc agcggcggca gggccgcgag cgtcccggac gggacgagga cctcgtagcc
      121 gagtccgccg gcggccacga cgacgcggtc gccggagcgc tccgccacgg tgccctcgac
      181 gaaggcgatc atcgccggga ccccgtgggg acgcgcgcct ggtggaggtg gcagacggcg
      241 acggcgacgg catcggccgc gtccggctcc gacggaacgc cgtcgatccg caggaggcgg
      301 ccgagcatcc gccgcacgtc ctccttgcga gcggtcccgt tcccggtgac cgcgagcttc
      361 acctcgagcg ggccgtactc gcggatctcg agcccgcgct cggcggcgac gagcaggagg
      421 acgccgctgg ctcgggaaac cgacatcgcg ctgccgacgt tgcgccccca ggcgagccgc
      481 tcgagcgcca tctcgctcgg cgcatgggcc cgcagggtgc tccgcagctc ctcggcgagg
      541 gcccggagcc gcgacgcctc cggcgcgccg gccggcgtcc ggatcgtccg cgcctcgagc
      601 acgctggggc ggtccccccc gcgcgtcccg acgaccgcga cccccgtcgc cgccacgccg
      661 gggtcgacac cgatcaccac ccggtcgaac acgtgttcag tctcaccgcg ggggggtgac
      721 gggtcaagga cctccgggga ccgccgctag gagacctcgg cgaggacctc gtcggggatg
      781 tcgaagttcg cgtggacgcc ctgcacgtca tcgagctcgt ccagggcgtc gatcagcgac
      841 aggacccgct tcgcctcggc gccggccacc ggcaccgtgg tgctcgggtt catcgtcagc
      901 tcagcggagg cgacgggcac gccgccggcg tcgagcaccg cgcgcatcgc ctggagctcg
      961 gtcggcgccg tcgtgatccg ccactggtca ccctcgcggg cgaggtcttc gccgcccgct
     1021 tccatgacga gctcgagcag gcgttcctcc tcgggcgcgg cgtccacgtc cacgaggagc
     1081 gtgcccttgc gctcgaactg ccacgcgacg ctccccgggt cgccgaggtt gcctcccatc
     1141 ttcgcgaagg tgtgtcgcac ctcctggccg gtgcggttgc ggttgtccgt cagggcctcc
     1201 acgaggatcg ccacgccgcc cggggcgtac ccctcgtaga cgacggggtc gtagcgggtt
     1261 ccggccatct cgccgctccc ccgcttgatc gcgcgctcga tgttgtccat cggcaccgag
     1321 tagtccttgg ccttctggac cgccgaggcg agggtcgcgt tcccgtcgag gttgccgccg
     1381 ccctcgcgcg ccgcgacctc gatcgcgcgc aggagcttcg cgaagagctt cccgcgacgc
     1441 tggtcgagca cggccttctt gtgcttgatc cccgcccact tcgaatgacc ggccatcagc
     1501 ccgccctctc cagcatccgt cgatggaggc tgccgtcgcc gaccagctcc gggtggaagg
     1561 ccgcggccag gagggagccc tgctcgacga cgacgggggt tccccccacc tccccaagca
     1621 ccgtcacagc cggacccacc cgctcgacca ccggtgcacg gatgaagacc ccgcgcacgg
     1681 gcccaacctg gtccacctcg agatccgtct cgaaggactc ggtctggcgg ccgtaggcgt
     1741 tgcgtgcgac gcggatgtcc atcagctcga gcaagggtat cccgccggcg acctcggcag
     1801 ccatgacgat catgccggcg caggttccga ggacgggcat cccggcgcca gcccggtcgc
     1861 ggatcggctc gacgaggccg cggctccttg cgagccgcga gatcgtggtg ctctccccgc
     1921 ccgggatgac gaggcacccg accgccgcga ggtccgccgg ggttcgcacg agcagcggcg
     1981 tcgcgccgag gcgttcgagg acgcgcgcgt gctcccggaa gtcgccctgc agggcgagga
     2041 caccagcctt cacgccggcc atgataccgg cggcctgcca ccgcggatga cccgttcgga
     2101 ctacctccga acggcccgac cgggccatgt ctcccgcccc ccgagcggac gaaactggtg
     2161 accgatggac tcgggcgtac gcatcctgcc gtatctgacg acgaccgggt tcgccgcgct
     2221 ggcggtcgcg gccgcggcct cgtgggtgca ccggcgcgag cccctccgcg gcgggttcgc
     2281 ccgcgcggcg ggtctgctcg ccgccgtgac cgcggtggag cgcatccacc aggcgacggc
     2341 ctaccgctcg accgcgctgg aggacgcatc catcgtcctg ttcctcgcct ccgggtacgc
     2401 gctcctcagc ctgtcccagc gcgtggtccc gatccgacgc tccgtcctgg tgctcctccg
     2461 cgcgctggtc gtgacggtgg cgttcgtcgg cgtggctgcg gagatgccga cgaagccgga
     2521 tgccccgatg tcggcgttcc agacgggact cgcctcctgc atcgtggccg tctgggcggc
     2581 ctgcgtgctc cttccgatcg ttcggttctg gcaggcgtcc cgcgggcgtc ccccggtgca
     2641 gcgcgcacgt ctgcgctcgc tggccctcgg ctacggcggc atcgtcctcg tgctgctcac
     2701 cgcgttcttc gcccactcct acgcgtccca ccccgccttc gtgggcggcg tccacctcgc
     2761 ggtcctcggc ctgatcccgc tcctgtacgc ggcgttcgtt ccgccccgct ggctccggcg
     2821 actgtggcgt gccacggaag aggccgcgac gggcgaggcg atgatccagc tgctcgaccc
     2881 gagcacggcg gccgacgatg tggccgcctc ggccgtccgc tggggcgcga ggctggtcgg
     2941 gggcgacgcc gcgttcctca cgcgcgacgg gcaggtccag gcggcgatcg gtctctccat
     3001 cgaggatgcc cgccggcacg cggcggacct cgcggacgtc cgtcaggcga cggtcgtgca
     3061 gatcgacgca cccgagcccc ggcacgcggt gcacctgccc gtgcgcgacg ccggtgggga
     3121 gggcgcgctg acggtgctgt cagggcggct caccccgctg ttcggggtgg acgagatcgc
     3181 agcgctcaac cggttcgctg cctcgatctg ggcggcgatc cagcacgcgt ccctcgtcga
     3241 ggccctgcgg gagcagacgg ctcgcgccga ggcgatggtg caggccgtcg gcgacctggg
     3301 cacgggcctg gtcgtggtcg aggacgcccg cttcgtcgac gcgaacgagg cctacctccg
     3361 gatgtccgga tacaccctgg aggagctgcg ggccctcccc tcggtgctat cgatcgccga
     3421 ccccgccgac ctcgcggagc tccaggagcg cctcgcgcga cgggccgccg gggcgccgat
     3481 cggcgaccac tacgagacgg cgctgatccg caaggacggc cgccgggtcg agatcgaggt
     3541 ctcgatcaag gacttcgagc gacggggccg gttgatcacg ctcgtcaggg acatcacggc
     3601 ccgcaagcag gccgaacgga ccctgcgcga cggctacgag cgggagcgag aggccgtcgc
     3661 gcgcctccgg acgctcgacg agatgaagag caccttcctc gccgccgtct cgcacgagct
     3721 gcggacgccg ctgaccgcga tctccggctt cgcacgcacc ctcgacggaa ggatggagga
     3781 gttcccagcg acggagcggg acgagatggt ccgagcgatc gcccgcaacg cggtccgcct
     3841 cgagcggctc ctcagcgatc tgctcgatct cgaccgcctc cagcggggtc tcctcgagcc
     3901 gcagggccgg cccacggacg tcggcggtct cgtccggcgg acgctcgtgg agtcggagct
     3961 cctggaaggt cgcgaagttt cggtcgacgc gggagaggtc ttcgccgtcg tcgacccgcc
     4021 gaaggtggaa cggatcgtcg agaacctggt cgggaacgcc gtccggcaca cccctgcgga
     4081 cacgccgatc tgggtccggg tctcggcgac ggagcgcgac gtgctgatcg tggtcgagga
     4141 cgccggcccc ggcgtgcccg cggagctgcg gacggcgatc ttccgcccct tcgagcaggg
     4201 ggaccgtggc gacgggcaca gccccggggt cgggatcggg ctctcgatcg tcgggcggtt
     4261 cgccgagctg cacgggggtc gcgcctgggt cgaggagcgt cccggcgggg gcgcctcgtt
     4321 ccgcgtcacg ctcccgctcg acggttcgga gcgcgagaga
//
LOCUS       DATLGG010000249         3877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_231430, whole genome shotgun sequence.
ACCESSION   DATLGG010000249 DATLGG010000000
VERSION     DATLGG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231430"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VM638_07135"
     CDS             complement(<1..326)
                     /locus_tag="VM638_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12231.1"
                     /translation="MARAAVPMPSEARMREVRRASGLPALVRLPAGPTRLPEDPLLRA
                     ADGADAGDFSDAARHLDGLADALRRASVGAPVAGLREDARLAVGGVGGLDGGWLGTIG
                     EHLRAW"
     gene            complement(445..1530)
                     /locus_tag="VM638_07140"
     CDS             complement(445..1530)
                     /locus_tag="VM638_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12232.1"
                     /translation="MAQQGGGGSELVGATTLAHRGRRYGLGPTVDGGYAIWDVTLRGA
                     PVRSFPGTPEGWNDAWHAYQELEASYLPPSRWTRGQPIVPQPMRAGQVLDAGVNLYRL
                     HFRTLMPLVAVVVIPVELLTLLISLQVPSSRAALVVQIGVIVLIALFSVALMTAAVLR
                     TAADAYIGQPPRIGSALRFALTRVHSILWVSLLVGLLVLIPMIPTVVAALARAPDALV
                     VILGIAGAAGVIAIAVTFMFAPPIVVVDDVRGSRALGRSRMLTRGFRWRIFGTLLLAY
                     LLAAVVAFVIALPFTPLTQGEITGELTTGGWIAVSIRDSLTQIITTPFTTVVTVLLYF
                     DARVRKEALDLEIMAQQLGEPAGEPGG"
     gene            complement(1557..2285)
                     /locus_tag="VM638_07145"
     CDS             complement(1557..2285)
                     /locus_tag="VM638_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12233.1"
                     /translation="MTALVPAIATAISLAFGIHLLVRFARRRRWAEGIWAIALLMYAT
                     ASGALTLGVMEPGWTPALFRTYWLFGAVLNVPYLALGEVYLLSRRPWVGHLGLAVVLA
                     ATAYATLVVRSAPVDPIISAAPPSLGPGEHGSGGAFYTGREVFGEGAPARTLAMIYSY
                     TGTAVLVLGILWSALGMRGRPDLRHRLHGTLLIAGGALVVAGGSAFAAAGNATGFSLT
                     LAVGVAVMYRGFLVATRRPAAPRA"
     gene            complement(2282..>3877)
                     /locus_tag="VM638_07150"
     CDS             complement(2282..>3877)
                     /locus_tag="VM638_07150"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVM12234.1"
                     /translation="GLASAPVSGTPGILSPVEPRVATLPDLLLGAAAEHGDRIALRSG
                     DRPIAYAEVAEWVCRAASGLAELGVRRGDRVAMLVGNVPEFVYALYGAQAAGAVVAPL
                     NVMLTPEEVGYILADADAKVVIAQIDYLPTVLAVRDRVASIEHVLVVGPPPTPRGTRS
                     FDELLDRARPRLPEVEVASEDLAVLAYTAGTTASPKGAMLTHGNLLANLEQISRVPAF
                     EEDASDVVLAALPLFHSYALNVVLGVTFMAGATAVLVERFDPLETLRLIERHHVTILF
                     GAPPMFHAWVEAAERAEADLSSVRLAVSGASALPERVLQAFRERFGMTIMEGYGLTEA
                     GPAVTTNAMGEARPGSIGLPLPGVEVRLLDEDGEEVADGDPGEILVRGPNIFLGYWGR
                     PEESEAVLRDGWLHTGDVAYRDDDGYLHVVDRKKDLVIVSGFNVFPKEVEDAILAHPD
                     VADCVVVGEPDERTGERVVAYVVAAPGAAVTEEEVREHCAGFLARFKVPRRVEVVDEL
                     PKHATGKVLRRELREAKHPEGVA"
BASE COUNT          608 a         1436 c         1389 g          444 t
ORIGIN      
        1 atccacgccc gcaggtgctc gccgatcgtc ccgagccagc ccccgtcgag tcccccgacc
       61 cctccgaccg ccagccgggc atcctctcgc aggcccgcga cgggagcacc gaccgacgcc
      121 cgccgcagcg catcggccag accatcgagg tgcctggccg catccgagaa gtcccccgca
      181 tcggcgccgt cggcggctcg caggaggggg tcctccggca gccgcgtcgg accggccggc
      241 agacgcacga gcgcggggag gccgctcgcc cgccggacct cgcgcatccg ggcctcgctc
      301 ggcatcggca ccgcggcgcg ggccatgtct gcggctcgct ccagtcggat gatcagctgc
      361 gccggcgaca cctcggccgg ccccgcgacc ccgagcgctg tcgggaggat cggtccggcc
      421 gcgatcagcg cgatgacggc gagctcagcc gcccggttcc ccagcgggct cccccagctg
      481 ctgcgccatg atctcgaggt cgagcgcttc cttgcggacc cgggcgtcga agtacagcag
      541 caccgtcacg acggtggtga atggcgtcgt gatgatctgc gtcaggctgt cgcggatcga
      601 cacggcgatc cacccgccgg tcgtcagctc cccggtgatc tccccctggg tcagcggggt
      661 gaacggcagg gcgatgacga aggcgacgac cgcggcgagc aggtaggcga gcaggagcgt
      721 cccgaagatc cgccagcgga accctcgggt gagcatccgg ctccggccga gagcgcgcga
      781 ccctcggacg tcgtcgacca ccacgatcgg gggcgcgaac atgaaggtga cggcgatcgc
      841 gatgacccct gcggcgccgg cgatcccgag gatcacgacg agcgcatccg gcgcgcgtgc
      901 caaagcggcg acgaccgtgg ggatcatcgg gatcaacacg agcaacccga ccaggaggga
      961 gacccacagg atcgagtgca cgcgcgtcaa cgcgaaccgc agggcagagc cgatcctcgg
     1021 cggctggccg atgtaggcgt ccgcagcggt gcgcaggacc gccgccgtca tcaacgcgac
     1081 ggagaacaac gcgatgagca cgatcacgcc gatctggacg accagcgccg cgcgcgacga
     1141 cggcacctgg agggagatga ggagcgtcag gagctcgacc gggatcacga cgaccgcgac
     1201 gagcggcatg agcgtccgga agtggagccg gtagaggttg acgccggcat cgaggacctg
     1261 gccggcgcgc atcggctggg ggacgatcgg ctggccgcgc gtccagcgcg acggcgggag
     1321 gtacgacgcc tcgagctcct ggtaggcgtg ccaggcgtcg ttccagccct ccggggtgcc
     1381 ggggaagctg cggaccggcg cgccccgcag ggtcacgtcc cagatcgcgt acccgccgtc
     1441 gacggtgggc ccgagcccgt accgccgtcc tcggtgggcg agggtggtcg cgccgacgag
     1501 ctcggaaccg ccaccgccct gctgtgccac cgggcgagga taccaggagg gctcgctcag
     1561 gcgcgcgggg cggcggggcg gcgcgtggcg acgaggaatc cccggtacat caccgcgacg
     1621 ccgaccgcga gcgtgaggga gaaaccggtc gcgttgccgg cggcggcgaa ggcggagccc
     1681 cccgcgacga cgagcgcgcc gccggcgatc agcagggtcc cgtggagccg gtgccgcagg
     1741 tcgggccgcc cgcgcatccc gagggctgac cacaggatgc cgagcacgag cacggccgtc
     1801 cccgtgtagg agtagatcat cgcgagggtc cgcgcggggg cgccctcacc gaagacctcc
     1861 cggccggtgt agaacgcacc tccgctccca tgctctcccg gccccaagga cggaggcgcg
     1921 gccgagatga tcggatccac cggggccgag cgcacgacga gcgtcgcgta cgcggtcgcg
     1981 gcgagcacga ccgcgagccc caggtggccg acccacgggc ggcggctgag caggtagacc
     2041 tcgcccagcg ccaggtaggg aacgttcagc acggcgccga agagccagta ggtccggaag
     2101 agcgccggcg tccagcccgg ctccatcacg ccgagggtga gcgctcccga agcggtcgcg
     2161 tacatcagca gcgcgatcgc ccagatcccc tcggcccagc ggcggcgccg cgcgaaccgc
     2221 acgagcaggt ggatgccgaa ggcgagcgag atcgcggtgg cgatcgccgg cacgagcgcg
     2281 gtcacgcgac cccctccgga tgcttcgcct cccgaagctc ccgccgcaac accttgccgg
     2341 tcgcgtgctt cggaagctcg tcgacgacct cgacgcgccg cggcaccttg aatcgcgcga
     2401 ggaacccggc gcagtgctcg cgcacctcct cctcggtgac ggcggctccc ggcgccgcga
     2461 cgacgtacgc gaccacccgc tcgcccgtcc gctcgtccgg ctcgccgacg acgacgcagt
     2521 cagcgacgtc cggatgcgcg aggatcgcgt cctccacctc cttcgggaag acgttgaacc
     2581 ccgagacgat cacgaggtcc ttcttgcggt cgacgacgtg gaggtagccg tcgtcgtccc
     2641 ggtaggccac gtcgccggtg tggagccagc cgtcgcgcag gacggcctcg ctctcctccg
     2701 gccgtcccca gtagcccagg aagatgttcg gcccgcggac gagtatctcg ccgggatcgc
     2761 cgtccgccac ctcttcgccg tcctcatcga gcagtcgcac ctcgacgccc gggagcggga
     2821 ggccgatcga acccggccgc gcttccccca tcgcgttcgt cgtcacggcg gggcccgcct
     2881 ccgtcagccc gtagccctcc atgatcgtca tgccgaaccg ctcgcggaag gcctggagca
     2941 cgcgctcggg aagcgccgac gcgcccgaga ccgccaggcg caccgaagac agatcggcct
     3001 cggcccgttc cgcggcctcc acccacgcgt ggaacatcgg cggcgcgccg aacaggatcg
     3061 tgacgtggtg ccgctcgatg aggcgcagcg tctccagcgg atcgaaccgc tcgacgagca
     3121 ccgccgtggc cccggccatg aacgtcacgc ccaggacgac gttgagcgcg tagctgtgga
     3181 acagcgggag cgccgccagg accacgtccg acgcatcctc ctcgaacgcc gggacacgcg
     3241 agatctgctc gaggttcgcg aggaggttcc cgtgcgtcag catggcgccc ttggggctcg
     3301 ccgtcgtccc ggccgtgtag gcgagcaccg cgaggtcctc gctcgccacc tcgacctccg
     3361 gcagccgcgg ccgcgcgcgg tcgaggagct cgtcgaagga acgcgtcccg cggggggtcg
     3421 gcggcggccc gacgacgagc acgtgctcga tcgacgccac ccggtcgcgg acggcgagca
     3481 ccgtggggag gtagtcgatc tgcgcgatga cgaccttcgc gtcggcatcg gcgaggatgt
     3541 agccgacctc ctccggggtc aacatcacgt tgagcggcgc caccaccgct ccggccgcct
     3601 gggcgccgta gagcgcgtag acgaactcgg ggacgttccc gacgagcatc gcgacccggt
     3661 ccccgcgacg gacacccagc tccgcgaggc cgctcgcggc ccggcacacc cattccgcca
     3721 cctcggcgta ggcgatcggc cggtcgccgg agcggagcgc gatgcgatcc ccgtgctcgg
     3781 cggcggcgcc cagcaggagg tccggcagcg tggcgacgcg aggttccacc ggcgacagga
     3841 taccgggggt cccgctcacc ggagcggaag ccagacc
//
LOCUS       DATLGG010000250         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_197727, whole genome shotgun sequence.
ACCESSION   DATLGG010000250 DATLGG010000000
VERSION     DATLGG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197727"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VM638_07155"
     CDS             complement(<1..452)
                     /locus_tag="VM638_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12235.1"
                     /translation="MRRVPSLVAGSLIALTACTTSVQTDQGSGAYVNEEAGFAIEGPE
                     GWVQQENVAGSTVSFLVPVPPEDGFAENINVIVQDAGPMGLEEYTELSLEELHGLGNG
                     AEVVEEGEAELGGQPAHRAVTRTNMEGRDLRILQLWTIHEGRAYVLTYT"
     gene            complement(496..897)
                     /locus_tag="VM638_07160"
     CDS             complement(496..897)
                     /locus_tag="VM638_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12236.1"
                     /translation="MKDLPRYDFPAISDAGDEERRVGPRLEEASFGESVIQEEVLYED
                     VGGEWRKGEWGERIGMVRLIQSPTTVGGKVLLRAWFLFDDGTTAEYAGLVPGEGTWQG
                     EGELGFRGGTGKFEGRRGHLHVESVNPKRWG"
     gene            986..>2344
                     /locus_tag="VM638_07165"
     CDS             986..>2344
                     /locus_tag="VM638_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVM12237.1"
                     /translation="MRPSLEETRKTVTVVFCDVVGSTSLGDRLDPEALRQVMTRFYVA
                     MREALERHGGSVQKYIGDAVMAVFGIPRLHEDDALRAVRAAQEMREALERLNEELAAG
                     WDVRLQTRIGINTGEVVVGDAAAGEALVLGDAVNVAARLEQAAGVGSVLMGAETYALV
                     RDHVEAEPVEPMELKGKPGLVHAYRLVAARPVEAPAMRPEPPLVGRSEELAVLRAGFD
                     RAVAKGSAVLATVLGPAGIGKSRLAREVVAGLDALVVTGRCLPYGDGITFWPIAEIVK
                     QASAVADDDPAEVARAKIEAAITGAEDAATVQERLAGLLGISDAPAAELQETFWAVRR
                     FLERLGERRPVVVVLDDLQWAEPTLLDLVEYLAGWTRQTALFLLCLARPDLLELRPSW
                     GTVGWPSSSVTLDPLDEQESRRLVSGLLEGLPLDDAAMDRIAAAAGGNALFLEEMLRM
                     LED"
BASE COUNT          302 a          855 c          837 g          350 t
ORIGIN      
        1 gtgtaggtga ggacgtaggc acggccttcg tggatcgtcc agagctggag gatccggagg
       61 tcccggccct ccatgttcgt cctcgtcacc gcgcggtgtg cgggctggcc gcccagctcc
      121 gcctcaccct cctcgaccac ctctgccccg ttgcccagcc cgtgcagctc ctccagcgag
      181 agctcggtgt actcctccag ccccatcggg ccggcgtcct ggacgatgac gttgatgttc
      241 tcggcgaagc cgtcctccgg cgggaccggg acgaggaacg agacggtcga tccggcgacg
      301 ttctcctgct ggacccaacc ttccggcccc tcgatcgcga acccggcctc ctcgttcacg
      361 tacgcgccgg atccctgatc ggtctggacg ctcgtggtgc acgccgtcag ggcgatcagg
      421 cttccggcca cgagtgaggg aacgcggcgc acggatgcca tcatcccgca ccgggcccgc
      481 gcgcgcgaac cgggatcagc cccagcgctt cgggttgacc gactcgacgt ggaggtggcc
      541 gcgacggcct tcgaacttcc ccgtcccccc gcggaagccg agctcaccct cgccctgcca
      601 cgtgccctct cccggcacga ggccggcgta ctcggccgtc gttccgtcgt cgaagaggaa
      661 ccacgcgcgg agcaggacct tgcccccgac cgtcgtcggt gactggatca accggaccat
      721 cccgatccgc tcgccccact cacccttccg ccactcgccg ccgacgtctt cgtagaggac
      781 ctcttcctgg atcacggatt cgccgaagct cgcctcctcg agcctcgggc cgacgcgccg
      841 ctcctcatcg ccggcgtcgc tgatcgctgg gaagtcgtac cgcggtagat ccttcacgat
      901 ggcccccttc gcccggtccc tcgcgcagcc tatggccgcc cctgcgacgg ggcaagcgag
      961 ggccgctcgg ccctagactc tccccgtgcg cccatccctg gaggagacgc gcaagaccgt
     1021 cacggtggtc ttctgcgacg tcgtgggctc cacgagcctc ggcgaccgcc tcgaccccga
     1081 ggccctgcgg caggtgatga cccgtttcta cgtggcgatg cgcgaggcgc tcgagcgcca
     1141 cggcggctcg gtccagaagt acatcggcga cgccgtcatg gcggtgttcg gcatcccgag
     1201 gctgcacgag gacgacgcgc tgcgggcggt ccgcgcggca caggagatgc gcgaggccct
     1261 cgagcgcctg aacgaggagc tggcggcggg ctgggacgtg cggctccaga cccggatcgg
     1321 gatcaacacc ggggaggtcg tggtcgggga cgcggccgcg ggcgaggccc tcgtcctcgg
     1381 cgacgccgtg aacgtcgcgg cgcgtctcga gcaggcggcg ggcgtgggga gcgtcctgat
     1441 gggcgctgag acgtacgccc tcgtgcggga ccacgtcgag gcggagccgg tcgagccgat
     1501 ggagctcaag gggaagcccg ggctcgttca cgcgtaccgc ctcgtcgcgg cgcggccggt
     1561 cgaggcgccc gcgatgcggc cggagccgcc gctcgtcggg cgcagcgagg agctggcggt
     1621 cctccgcgcg gggttcgacc gcgcggtcgc gaagggctcg gcggtcctcg cgacggtcct
     1681 cgggcctgcc gggatcggga agtctcgcct ggcgcgtgag gtcgtcgcgg ggctcgacgc
     1741 cctggtggtg acgggacgct gcctccccta cggggacggg atcacgttct ggccgatcgc
     1801 cgagatcgtg aagcaggcat cggccgtcgc cgacgacgat ccggccgagg tcgcgcgcgc
     1861 caagatcgag gcggcgatca ccggtgcgga ggatgccgcg accgtgcagg agcggctcgc
     1921 gggcctgctc gggatctcgg atgctcctgc ggcggagctg caggagacct tctgggcggt
     1981 ccggcgcttc ctcgagcgac tcggcgagcg acggccggtc gtggtggtcc tcgacgatct
     2041 ccagtgggcc gagccgacgc tcctcgacct cgtcgagtac ctcgccggct ggacgcggca
     2101 gaccgcgctg ttcctcctct gcctcgcgcg gcccgacctg ctcgagctgc ggccgagctg
     2161 ggggaccgtc gggtggccgt cgagctcggt caccctggac ccgctcgacg agcaggagag
     2221 ccgccgcctc gtctccgggc tgctcgaggg gctcccgctc gacgatgcgg ccatggaccg
     2281 gatcgccgcg gccgccggcg gcaacgcgct cttcctcgag gagatgctgc ggatgctcga
     2341 ggac
//
LOCUS       DATLGG010000251         9946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_120545, whole genome shotgun sequence.
ACCESSION   DATLGG010000251 DATLGG010000000
VERSION     DATLGG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120545"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="VM638_07170"
     CDS             <1..1708
                     /locus_tag="VM638_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVM12238.1"
                     /translation="PITVTVHESDRGLVATDLARRFPLSGGFPAGGELHDLHVLAYPG
                     DGASIARRPDVLYVGPAPAGIGLLDEMSDQVQAGNVSGATVFTGYETWLATHGLSGAG
                     QVVSITDSGIDPVHPDLRGRIRARIDYAPLGQTDSPVDEPVDTLGHGTHVAGIVAGDA
                     NGLQPVTRAGDGNGFLYGLGVAPKAELVDQNLLSTTASAIFCPINGIGFWPPPYPWDR
                     LTRDALANGASLWNGSWWSCEDSSGRYIATNRTFDQLTRDADPTTSGNQPFTFVFAAA
                     NFGPSSRTVAAPASAKNVISVGATLNQRAGNIDTIADFSSRGPAFDTRILPTVSAPGD
                     GVISTRSSASTAACSVPVANGFTLYSSCRGTSMASPHVAGAVALLREWWRKSNPVDPS
                     PAMTKALLVNTATDMGVADIPNPNEGWGRVNLGTLFAPDTRVYEDQTTVLTELDETAT
                     YRVRAADPSKPLKITVAWTDPAGAAGANPALVNDLDLRVVSESGAAYWGNRFNAGRST
                     TGGAPNRRDNLEAVFLASPGDQVHDVSITAYNLPGDGAPGGDQTDQDFALVISNGVIV
                     AG"
     gene            1817..2920
                     /locus_tag="VM638_07175"
     CDS             1817..2920
                     /locus_tag="VM638_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12239.1"
                     /translation="MRKIVALLSLIVATTPAVAPATDAMPAGATDPLGSLRWLPRGAN
                     ERPQGHSSRLVVVYDRSLDDGGVVPLRAKGPFVVGLSTTTVGQMTIAGHSPSVSVARG
                     DTGSPEAEFVLHAVDGYLGAWTGDIRDGDCAKAGTGAFSAAYSTTRSFFHPGAAPYAP
                     STTAGGCEVVDAATAVRDSAGRHLGFDTIVTTHVPGFWIDVFYSELSTATFRPGRANA
                     DIWYEAAYDEEGEEGLWHEVADAAGISTAVDAPYDQILTFRATPGGTATPCLSLTDPT
                     LPGSFGERSVHVPVGTSSVTFQLFPKGSWEMRVIDPSGQFRSGLAVPGRRQVISAPGS
                     GTFDFPVLRPGWFTVRACNIAGEHEVVAAVTFG"
     gene            complement(2924..3334)
                     /locus_tag="VM638_07180"
     CDS             complement(2924..3334)
                     /locus_tag="VM638_07180"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HVM12240.1"
                     /translation="MTFDVLGEINYLAVLAAAVAYFAFGALWYTALFGRAWQRATGVT
                     QSDAGSMATAFIGSFIGYFISAVALAAIARATGSSTVMHGIVIGSMVGLGLLTPMIWI
                     NATYERRPATLFWINAMNAVLGYTLMAIIVTVWR"
     gene            3422..3853
                     /locus_tag="VM638_07185"
     CDS             3422..3853
                     /locus_tag="VM638_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12241.1"
                     /translation="MDDASDLQQLTIDYLLLADGAQVQNGKLYVLGGGWERLQFDSYP
                     QTVPVGIALGVRVPWGETNQKHSFRIRGLDADGQELFGGQGEFEMGRPAGLPLGTSQI
                     FQVALQLPLPVPAPGQYSVEADVDGAVRRSTPYFAVQTRPG"
     gene            3850..8211
                     /locus_tag="VM638_07190"
     CDS             3850..8211
                     /locus_tag="VM638_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVM12242.1"
                     /translation="MSLAPFSAPVRAWFETTFPEPTPAQAQGWPAIISGEHSLILAPT
                     GSGKTLAAFLTAIDRLGTTPPVIPERRCRVLYISPLRALAVDVEKNLRTPLAGISLAA
                     ERLGDGFVQPEVGIRTGDTPATERRALVRHPPDILITTPESLYLMLTSQAREMLRGVE
                     TVIVDEIHALAGNKRGAHLALSLERLEELTERPPQRIGLSATQRPLEEIARFLGGRTS
                     DGPRPVRIVDAGIRKPIEVEVVVPVEDMGRLGDVEPDLVSGPASSGPTRMSIWPAVHP
                     KILELIRAHRSTIVFCNARRLAERLASRLNELAGEELVRSHHGSLAREQRVIVEDQLK
                     RGELRGIVATSSLELGIDMGAVDLVVQVESPGSVARGLQRIGRAGHRVGEPSRGRIFP
                     KYRGDLVEAAVVVRRMRDGSIEETRVLRNPLDVLAQQIVAIVAMDEWRLHDLAALVRR
                     AAPFSDLSDEVLRSVLDMLAGRYPSEEFSELRPRLVWDRRDDTLRAREGAQRLAVTSG
                     GTIPDRGLFGVFLPDGARVGELDEEMVYESRPGETFLLGASTWRIEEITHDRVIVTPA
                     PGEPGKMPFWHGEGPGRPLELGRALGAFVRAARSGEADLEHDYGLDRLAAENLLRYLR
                     EQEEAAGAVPDDRTIVVERFRDEIGDWRVCVLSPFGARVHAPWAIAVQARLEERLGLQ
                     VEALWSDDGIVFRLPEAEEHLPLEDLLLDPEDVEDLVLSRVTGTALFASLFRDAAGRA
                     LLLPRRRPDRRTPLWQQRRRAADLLAVASRYPDFPILLETTRECLQDHFDLPALLQVL
                     TDIRTRRIRVAAAETQQASPFAQSLLFDWIAVYMYEGDAPLAERRAQALALDRDLLRE
                     LLGAEELRDLIDEDVLEELELELQGLVPERRARSADELHDVLRRLGPLLDEDLAVRAG
                     GDAATWIAELLEQKRAIRVTFADGDRVAAAEDAARLRDALGVALPLGLPSAFTDPVER
                     PLDGLVGRYARTHGPFETEQVADAFGLAVDRVVPALERLEADGRLVRGEFRPGGTRRE
                     WCDVEVLRVLRRRSLARLRKEVEPVEAEVLGRFLPAWQGVGSARRGMEALAEVINQLQ
                     GAAIPASVLEVDVLAQRLQGYRPADLDELCASGDVVWSGAGPIGASDGRVALAFRDHL
                     DVLPPVPPEPPDGDLHEALRSHLAAQGASFWPDLVRAAGTGDEGAVLAALWDLVWAGE
                     VTNDTPAPLRAFLGRRPAARRGRRARPGSLRRVGPPAGAGRWSLLRRPPVDPTVVAHA
                     RALQLLERHGVLTREAAGVGSLPGAFSGIYPVLRQLEEAGRIRRGYFVSGLGGAQFAL
                     PGAIDRLRTLRDRPDEPIVHALAATDPAQPYGAALPWPDAEGRPARTAGAYVVLVDGA
                     ATAYLERGARSLLTFPAASERDGWADGLASLVKDGRLARIELRRIDGGPADASPVAER
                     LRSAGFSDGYRGLTLRG"
     gene            8216..8992
                     /locus_tag="VM638_07195"
     CDS             8216..8992
                     /locus_tag="VM638_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HVM12243.1"
                     /translation="MPEGDTIHRAASTLDRALTGRALTAFRADGLGGALPGPGTTVRG
                     VQARGKHLLIRFDDGLTLHTHMRMEGSWHLYRPGERWRKARASAKIVLETEDWVAVCF
                     SAPIVEVVRDERRLASVRSLGPDLTAPDPDLDEALTRMAATEQEREIGEVLLDQRIAA
                     GIGNVYKSEVLHLERISPFTPLSGLEVAARRRLLERAAALLRRNLTTVRRTTVAGGLA
                     VYGRAGRPCRRCGSTIRRRLQGRERPRATFWCPACQPSAA"
     gene            complement(8989..9645)
                     /locus_tag="VM638_07200"
     CDS             complement(8989..9645)
                     /locus_tag="VM638_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HVM12244.1"
                     /translation="MDVLAHIDRLIAERHLLTHPFYEKWQSGTLPKGALQDYARQYYA
                     FESAFPTILSAIHSRTEDPVTRQHLLENLWDEEHGPQNHAELWLRFAEGVGVSRESVR
                     GAAPTAQTRDLVATYRRSATTAPVPAAVAGVYAYESQVPAVAEKKIAGLREHFGVDDP
                     SALSFFTVHQALDVEHAGAERTLIERLAAGHEEEVIAATSDALEAWWGFLDGVDPEGC
                     "
     gene            complement(9748..>9946)
                     /locus_tag="VM638_07205"
     CDS             complement(9748..>9946)
                     /locus_tag="VM638_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12245.1"
                     /translation="NLAPLWSHTKVVDSGYPSWSLYLNGGALRRDAGHDLIAEYVQGG
                     PGRHAAEVFEGATGAVRWTSP"
BASE COUNT         1319 a         3643 c         3553 g         1431 t
ORIGIN      
        1 gccgatcact gtcaccgtgc acgagtcgga ccgcgggttg gtcgcgacgg atctcgcgcg
       61 ccggttcccg ctgtcgggcg gcttcccggc gggcggtgag ctccacgatc tccacgtttt
      121 ggcctacccg ggggatgggg cttcgatcgc ccgccggccg gacgtgctgt acgtcggccc
      181 cgcacccgcc gggattgggc tcctcgacga gatgagcgat caggtccagg ccggcaacgt
      241 gagcggcgcc accgtgttca cgggctacga gacgtggctg gcgacgcacg ggctgagcgg
      301 cgcgggccag gtcgtgtcga tcaccgactc gggcatcgat cccgtccacc ccgacctgcg
      361 cggtcggatc cgcgcgcgga tcgactacgc cccgctcgga cagaccgaca gtcccgtgga
      421 cgagccggtc gatacgctgg gccacggcac ccacgtcgcg gggatcgttg cgggcgacgc
      481 gaacgggctg cagccggtga ctcgcgcggg ggacgggaac gggttcctct acgggctcgg
      541 ggtggcgccg aaggcggagc tcgtggacca gaacctgctg tccaccacgg cgagcgcgat
      601 cttctgtccg atcaacggca tcgggttctg gccgccaccg tacccgtggg accgtctgac
      661 gcgcgacgcc ctggccaatg gcgccagcct ctggaatgga tcgtggtggt cctgcgagga
      721 cagctccggg aggtacatcg cgacgaaccg tactttcgac cagctcaccc gtgatgctga
      781 tccgacgacg tcaggcaacc agccgttcac gttcgtcttc gccgcggcga acttcggccc
      841 gtcgtcccgc accgtggcgg caccggcgtc ggcgaagaac gtgatatcgg tgggtgccac
      901 cctcaaccag cgggcgggga acatcgacac gatcgccgac ttctccagcc gcggtcccgc
      961 cttcgacacg cggatcctcc cgacggtgtc ggcgcctggt gacggggtca tctcgacgcg
     1021 gtcgagcgcg agcaccgcgg cgtgcagcgt ccccgtcgcg aatgggttca cgctgtactc
     1081 ctcgtgccgg ggaacctcga tggcctcgcc gcacgtcgcc ggcgcggtcg cgctcctgcg
     1141 cgagtggtgg cggaagtcga accccgtgga cccatcgccc gcgatgacga aggcgctgct
     1201 cgtgaacacg gcgaccgaca tgggcgtcgc ggacatcccc aacccgaacg agggctgggg
     1261 ccgtgtgaac ctcgggacgc tgttcgcccc ggacacgcgc gtctacgagg atcagacgac
     1321 ggtgctgacc gagctggacg agacggcgac ctaccgggtg cgtgcggccg acccgtcgaa
     1381 gccgctgaag atcacggtcg cctggaccga tccggccggc gccgccggcg cgaaccccgc
     1441 cctcgtcaac gacctcgacc tccgcgtcgt ctcggagagc ggcgctgcct actgggggaa
     1501 ccggttcaac gcagggcgct cgaccaccgg tggggcgccg aaccgacgag acaacctcga
     1561 ggcggtcttc ctcgcctcac cgggggacca ggtccacgac gtctcgatca ccgcgtacaa
     1621 cctccccggt gacggcgccc ccggcggcga tcagacggac caggacttcg ccctcgtgat
     1681 ctccaacggt gtgatcgtgg cgggataacc gccaccacgg cggcgttatc gaaggagcgg
     1741 gcggccggta tccccccggg aaagaccagc cgcccgcttc tgcgtcccgg aagcgagccc
     1801 ggatccggga gtcgtcatga ggaagatcgt cgcccttctg agcctcatcg ttgccacgac
     1861 ccccgccgtc gctccggcga cggatgccat gcccgccgga gccaccgacc cgctcgggag
     1921 cctgcgttgg ctgccgagag gagcgaacga gcggccccag gggcacagct cgcgcctcgt
     1981 ggtcgtctac gaccggtcgc tggacgacgg aggtgtcgtt cccctgcgtg ccaagggccc
     2041 cttcgtcgtg ggcctctcga cgaccaccgt agggcagatg acgatcgccg gtcactcgcc
     2101 gtcggtcagc gtcgcacgtg gggacacggg tagccccgag gcggagttcg tgctccatgc
     2161 tgtggacggg tatctcggtg cctggaccgg ggacatccgt gacggcgact gtgcgaaggc
     2221 cggaaccggg gccttctcgg cggcctactc gacgacgcgc tcgttcttcc atcctggggc
     2281 cgctccgtac gccccttcga cgaccgccgg cggctgcgag gtcgtcgacg cggcaaccgc
     2341 ggtcagggac agcgccgggc gccatctcgg cttcgacacc atcgtgacca cccacgtccc
     2401 cgggttctgg atcgacgtct tctacagcga gctctcgacg gcgacgttcc gccccggccg
     2461 cgccaacgcg gatatctggt acgaggccgc gtacgacgag gagggggagg aggggctgtg
     2521 gcacgaggtg gctgatgcgg ccggcatctc gacggctgtc gacgccccct acgaccagat
     2581 cctcacgttc agggcgaccc ccggcgggac ggcgaccccc tgcctgtccc tcaccgaccc
     2641 gacgctcccg ggaagcttcg gcgagcgctc cgtccacgtg cccgtcggca cgtcgagcgt
     2701 caccttccag ctcttcccga aggggagctg ggagatgcgc gtgatcgatc cgagcggcca
     2761 gttccgctcg gggctggcgg tcccggggcg acggcaggtc atctcggcgc cggggagcgg
     2821 gaccttcgac ttcccggtcc tgcgcccggg ctggttcacc gtgcgcgcct gcaacatcgc
     2881 cggcgagcac gaggtcgtcg ctgccgtgac cttcggctga gggctatcgc cagacggtga
     2941 cgatgatcgc catgagcgtg tagccgagga cggcgttcat cgcgttgatc cagaagagcg
     3001 tggccggacg ccgctcgtag gtcgcgttga tccagatcat cggcgtgagc agtcccaggc
     3061 cgaccatcga tccgatcacg atcccgtgca tcacggtcga ggacccggtg gcccgcgcga
     3121 tcgccgcgag ggccaccgcc gagatgaagt agccgatgaa cgacccgatg aaggcggtcg
     3181 ccatcgaacc ggcatcggac tgcgtgacgc cggtggcgcg ctgccaggcc cggccgaaca
     3241 gggccgtgta ccacagggcg ccgaacgcga agtacgcgac cgcggccgcc aggaccgcca
     3301 ggtagttgat ctcgccgagc acgtcgaacg tcatccgagc ctcctccatc cggtcgtcgc
     3361 ccccgttcta gaccttgctc cgctcgggca caagcccccc gcgcgtatgc tggtccttgc
     3421 gatggacgac gcgagcgacc tgcagcagct cacgatcgac tacctcctgc tggccgacgg
     3481 cgcgcaggtg cagaacggga agctctacgt gctcggcggc ggctgggagc ggctccagtt
     3541 cgactcgtac ccgcagacgg tgccggtcgg catcgcgctc ggcgtccgcg tgccctgggg
     3601 ggagacgaac cagaagcact cgttccggat ccgaggcctc gacgcggacg ggcaggagct
     3661 cttcgggggc cagggcgagt tcgagatggg ccgaccggcc ggtctcccgc ttgggacctc
     3721 gcagatcttc caggtggcgc tgcagctccc gctcccggtc cccgctccgg gccagtactc
     3781 ggtcgaagcc gacgttgacg gggcggtccg gcgctcgacg ccgtacttcg cggtgcagac
     3841 ccgcccggga tgagcctcgc gccgttctcc gcgcccgtcc gtgcctggtt cgagacgacg
     3901 ttcccggagc cgacgccggc gcaggcgcag ggttggccgg ccatcatctc gggcgagcat
     3961 tcgctgatcc tcgccccgac cggctcgggc aagaccctcg cggcgttcct cacggcgatc
     4021 gaccggctcg ggacgacacc ccccgtcatt cccgagcgcc ggtgccgcgt gctctacatc
     4081 tcgcccctcc gcgcgctcgc ggtggacgtc gagaagaacc tgcgcacgcc gctcgccggc
     4141 atcagcctcg cggcggagcg gctgggggac ggcttcgtgc agccggaggt cgggatccgc
     4201 accggagaca cgcccgcgac ggaacgacgc gcgctcgtcc gccaccctcc ggacatcctc
     4261 atcacgacgc cggagtcgct gtacctgatg ctcacgtcgc aggcgcggga gatgctgcgc
     4321 ggcgtcgaga ccgtgatcgt cgacgagatc cacgccctcg cggggaacaa gcgaggcgcg
     4381 cacctcgcgc tcagcctcga gcggctggag gagctgaccg agcgcccgcc gcagcgcatc
     4441 gggctgtcgg ccacccaacg cccgctcgag gagatcgcgc ggttcctcgg cgggcgaacc
     4501 tccgacggtc cacgccccgt ccgcatcgtc gacgcgggga tccgcaagcc gatcgaggtg
     4561 gaggtcgtcg ttcccgtcga ggacatgggc cggctcgggg acgtggagcc ggatctcgtc
     4621 tccggcccgg cctcctccgg tcccacgcgg atgagcatct ggcccgcggt ccacccgaag
     4681 atcctcgagc tgatccgcgc gcaccgctcg acgatcgtgt tctgcaacgc gcgccggctc
     4741 gccgagcgcc tcgcgtcgcg cctgaacgag ctggccggtg aggaactggt ccgctctcac
     4801 cacgggtccc tcgcgcgcga gcagcgcgtg atcgtcgagg accagctcaa gaggggggag
     4861 ctgcgcggga tcgtcgcgac ctcctcgctc gagctcggga tcgacatggg tgccgtcgac
     4921 ctcgtcgtgc aggtcgagtc gccggggtcg gtcgcgcgcg ggctgcagcg gatcgggcgg
     4981 gccggtcacc gcgtcggcga gccgtcgcgc gggcggatct tcccgaagta ccgcggcgac
     5041 ctcgtggagg cggcggtcgt cgtccggagg atgcgcgacg gctcgatcga ggagacccgt
     5101 gtcctgcgga acccgctcga cgtgctcgcc cagcagatcg tcgcgatcgt ggcgatggac
     5161 gagtggcggc tgcatgacct ggccgcgctc gttcggcgcg ccgccccctt ctcggacctc
     5221 agcgacgagg tcctgcgttc ggtcctcgac atgctggccg ggcgctatcc ctcggaggag
     5281 ttctccgagc tccgcccgcg tctcgtgtgg gaccggcgtg acgacacgct ccgcgcgagg
     5341 gagggggcgc aacggctggc ggtcacgagc ggtgggacga tccccgaccg cggcctgttc
     5401 ggcgtcttcc ttccagacgg cgcgcgggtc ggagagctcg acgaggagat ggtgtacgag
     5461 tcgcgcccag gcgagacgtt cctcctcggc gcgtcgacgt ggcggatcga ggagatcacc
     5521 cacgaccggg tgatcgtgac gccggcgccg ggggagccgg ggaagatgcc gttctggcac
     5581 ggcgaggggc cgggccggcc gctcgagctc gggcgcgccc tcggagcgtt cgtgcgcgcc
     5641 gcccgcagcg gggaagccga cctcgagcat gactacgggc tggaccggct cgccgccgag
     5701 aacctcctgc ggtacctccg tgagcaggag gaggcggcgg gcgcggtgcc cgacgaccgg
     5761 acgatcgtcg tcgagcgctt ccgggacgag atcggcgact ggcgcgtgtg cgtgctgtcg
     5821 ccgttcgggg cacgcgtcca cgcgccctgg gcgatcgcgg tgcaggcccg gctcgaggaa
     5881 cgcctcggtc tgcaggtcga ggcgctgtgg agcgacgacg gcatcgtctt ccggctgccg
     5941 gaggccgagg agcacctccc gctcgaggat ctgctcctcg accccgagga cgtcgaggat
     6001 ctcgtcctct cccgggtgac cgggacggcc ctgttcgcct cgctcttccg cgatgcggcc
     6061 ggccgggcgc tcctgctccc gcgacgccgc cccgaccgtc gcaccccgct gtggcagcag
     6121 cgccgccgag ccgccgacct gctcgcggtg gcttcccgct acccggactt cccgatcctg
     6181 ctcgagacga cgcgcgaatg cctccaggac cacttcgacc tccccgcgct cctgcaggtc
     6241 ctcaccgaca tccggacgcg ccggatccgc gtcgccgccg ccgagacgca gcaggcctcg
     6301 ccgttcgcgc agtcgctgct gttcgactgg atcgccgtct acatgtacga gggcgacgcg
     6361 ccgctggcgg agcggcgcgc gcaggccctc gcgctcgacc gcgacctcct gcgtgagctg
     6421 ctcggcgccg aggagctccg cgacttgatc gacgaggacg tcctcgagga gctcgagctg
     6481 gagctccagg gcctcgtccc cgaacggcgc gcgcgcagcg ccgacgagct ccacgacgtc
     6541 ctccgccgcc tcggcccgct cctcgacgag gacctcgccg tccgcgccgg cggtgacgcc
     6601 gcgacgtgga tcgccgagct cctcgaacag aagcgcgcga tccgcgtgac gttcgcggac
     6661 ggggatcggg tcgccgccgc cgaagacgcc gcacgcctgc gggacgcgct tggcgtcgcc
     6721 cttccgctcg gtctccccag cgcgttcacc gacccggtcg agaggccgct cgatgggctc
     6781 gtcgggcgct atgcccgcac gcacggccca ttcgagaccg agcaggtcgc ggacgcgttc
     6841 ggcctcgcgg tcgaccgggt cgtccccgcg ctggagcgcc tcgaagccga cggcaggctc
     6901 gtccgtggcg agttccgccc cggcggcacg cgacgcgaat ggtgcgacgt cgaggtcctc
     6961 cgggtcctgc gccggcgctc gctcgcgcgc ctgcgcaagg aggtcgagcc ggtcgaggcg
     7021 gaggtcctcg ggcggttcct cccggcgtgg cagggcgtcg gctcggcgcg ccgtgggatg
     7081 gaggccctcg ccgaggtcat caaccagctc cagggcgcgg cgatcccggc gagcgtcctc
     7141 gaggtcgacg tcctcgccca gcggctccag gggtaccggc ccgccgacct cgacgagctc
     7201 tgcgcctccg gcgacgtcgt ctggagcggg gcaggtccga tcggcgcctc cgacggccgg
     7261 gtcgccctcg cgttccgcga ccaccttgac gtgcttccgc ccgtcccacc cgagccgccc
     7321 gacggggacc tccacgaggc gctccgctcc cacctggccg cccagggcgc ctcgttctgg
     7381 ccggacctgg tgcgggcggc cgggaccggc gacgaggggg ccgtcctcgc ggcgctgtgg
     7441 gacctcgtct gggcgggcga ggtgacgaac gacactcccg cgccactgcg ggccttcctc
     7501 ggccgccgcc cggcggcacg gcggggacgc cgcgcccgtc ccggcagcct gcggcgcgtc
     7561 ggtcctcccg ccggcgccgg ccgctggtcg ctcctgcggc gaccgccggt cgatccgacg
     7621 gtcgtcgcgc acgcgcgagc cctccagctg ctggagcgcc acggcgtcct gacgcgggag
     7681 gcggcggggg tgggttcgct tccgggcgcc ttctccggca tctaccccgt cctgcggcag
     7741 ctcgaggagg cggggcggat ccggcgcggc tacttcgtgt cgggcctcgg cggtgcgcag
     7801 ttcgccttgc ccggtgcgat cgaccggttg aggacgctcc gcgaccgccc ggacgagccg
     7861 atcgtccacg cgctggccgc gaccgacccc gcccagccct acggcgccgc tctgccgtgg
     7921 cccgacgccg agggtcggcc ggcgcggacg gccggggcct acgtcgtcct cgtcgacggc
     7981 gcggcgacgg cgtacctcga gcgcggcgcg cgcagcctgc tgaccttccc ggccgcctcc
     8041 gagcgtgacg ggtgggccga cggcctcgcg agcctcgtga aggacgggag gctcgcgcgc
     8101 atcgagctcc gtcgcatcga cggcggtcct gccgacgcat ctccggtggc cgaacggctg
     8161 cgctccgccg gattctccga cggctaccgg gggctcacgc tccgaggctg aagcgatgcc
     8221 cgaaggcgac acgatccacc gcgcggcctc gacgctcgac cgcgccctga cgggacgggc
     8281 gctgaccgcg ttccgcgccg acggcctcgg cggggctctt cccgggcccg gcacgacggt
     8341 ccgcggggtc caggcgaggg gcaagcacct cctgatccgc ttcgacgacg gtctcacgct
     8401 gcacacgcac atgcggatgg aggggtcgtg gcacctctac cgcccggggg agcggtggcg
     8461 gaaggcgcgt gccagcgcca aaatcgtcct ggagaccgag gactgggtgg cggtctgctt
     8521 ctcggcgccg atcgtcgagg tcgtgcggga cgagcgacgg ctggcgtcgg tccggtccct
     8581 cggccccgac ctcaccgctc cggatccgga cctcgacgag gcgctgaccc ggatggcggc
     8641 gacggagcag gagcgtgaga tcggcgaggt gctgctcgac cagcgcatcg cggcagggat
     8701 cggcaacgtc tacaagagcg aggttctcca cctcgagcgc atctcgccct tcaccccgct
     8761 cagcgggctc gaggtggctg cgcggcggcg tttgctggag cgtgccgccg cgctcctgcg
     8821 gaggaacctg acgaccgtgc gtcggacgac ggtcgccggc ggcctcgccg tgtacgggcg
     8881 ggcggggcgc ccctgccgcc gctgtggttc aacgatccgg cggcgtctcc aggggcgcga
     8941 gcgtccgcgc gcgaccttct ggtgcccggc ctgccagccg tcggccgctt agcagccctc
     9001 ggggtcgacg ccgtcgagga agccccacca cgcctcgagc gcatccgatg tcgccgcgat
     9061 cacctcttcc tcgtgacccg ctgcgagccg ctcgatgagt gtccgctcgg ctcccgcgtg
     9121 ctcgacgtcg agggcctggt ggacggtgaa gaaagagagc gcgctggggt cgtcgacccc
     9181 gaagtgctcg cgcagaccgg cgatcttctt ctcggcgacg gccgggacct gcgactcgta
     9241 ggcgtagacc cccgcgacgg cggcgggaac cggcgccgtg gtcgcgctcc gccggtacgt
     9301 cgcaacgaga tcgcgggtct gcgccgtggg tgccgccccg cggacgctct cccgggagac
     9361 gccgaccccc tcggcgaagc ggagccacag ctccgcgtgg ttctgcggtc cgtgctcctc
     9421 gtcccagagg ttctccagga ggtgctggcg cgtcacgggg tcctcggtcc ggctgtggat
     9481 cgccgagagg atcgtcggga acgccgactc gaacgcgtag tactgccggg cgtagtcctg
     9541 cagcgctccc ttcggaagcg ttccgctctg ccacttctcg tagaacgggt gggtgagcag
     9601 atgccgctcc gcgatcagtc ggtcgatgtg cgcgagcacg tccacgggct cctcctcggt
     9661 tcggtgtcgg cgtagcctgt cagccgcggc cccagcccgc aacccagcgg agtgcgatca
     9721 cgcctcgcat gatccaaggg gcgcccatca ggggctcgtc cacctgaccg cgccggtcgc
     9781 gccctcgaag acctccgccg cgtgccgccc cggcccgccc tgcacgtact ccgcgatcag
     9841 gtcgtgtccc gcatcgcggc ggagcgcccc gccgttcagg tagaggctcc agctgggata
     9901 cccggagtcc acgaccttcg tgtgcgacca gagcggggct aaattg
//
LOCUS       DATLGG010000252         8043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_62211, whole genome shotgun sequence.
ACCESSION   DATLGG010000252 DATLGG010000000
VERSION     DATLGG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62211"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VM638_07210"
     CDS             <1..1016
                     /locus_tag="VM638_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM12246.1"
                     /translation="LVEGELVQLSAVSSGVADARKVIERARGSMFSTILFVDEVHRWS
                     KAQQDVLLPAVEEGTVTLIGATTENPYFSLVSPLLSRCLLLRLEPLDAKDLRDLLERA
                     LRDPERGMGGDDLEADEEALTFLADAAGGDARFALSGLEAAALAARGAGERKIGRSLV
                     EDAVQRRALVYDRDEHYDVISAFIKSLRGSDPDAGLFYLARMLEAGEDARFIARRMVI
                     LASEDIGPADPQSLLIANAAAHAVEYVGLPEAELNLAQAVIHLARAPKSNAAYRALGA
                     AKREAADPRHRVPPHLRDTHYRGAEALGHGAGYVYPHDDPDGARDQRYLPETARGGYL
                     EDR"
     gene            1064..1420
                     /locus_tag="VM638_07215"
     CDS             1064..1420
                     /locus_tag="VM638_07215"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HVM12247.1"
                     /translation="MTGGEWALLIAAIAWAVLVVFLAIVLANLSRVLDSARTMIDGVH
                     QETIPLLTEVTTSVKKVNRELDRVDPLLASAANITRSIERLTAVLERLLASPLIKAAA
                     VGAGVTRAIRRFRGEK"
     gene            1417..1653
                     /locus_tag="VM638_07220"
     CDS             1417..1653
                     /locus_tag="VM638_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12248.1"
                     /translation="MRRAFWVALGLGAGVTSAILVSRWLRRQRERFSPSNIGAQVGEG
                     VRDLRQLLGVSLEEGRRAMREHEAEIRSAIDPDA"
     gene            complement(1650..2414)
                     /locus_tag="VM638_07225"
     CDS             complement(1650..2414)
                     /locus_tag="VM638_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM12249.1"
                     /translation="MRRTLRQLRYEHRAFRRNPASAVFTFAFPLMFLVIFNLIFGNLE
                     IRVAGGTTTASTFYVPAIAAFSIITTCFTNLAMRLTFDRDQGRLKRIRGTPLSTGAFL
                     AGKILHVIALAIVLVAIVTLFGMIFYGVRAPTGSLPAVLVTLAVGAAAFCALGIAITA
                     AIPNADAAPAVVNATILPLLFVSDVFIRLDQAPPWLRTLGDLFPVKHLSQALQTAYDP
                     FLSGPGFEWGHLGAIAAWGAAGLVVALRTFSWEPRR"
     gene            complement(2411..3304)
                     /locus_tag="VM638_07230"
     CDS             complement(2411..3304)
                     /locus_tag="VM638_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12250.1"
                     /translation="MSELAIEVRDLRKRYGQIEAVRGLDLAIRRGEIFALLGPNGAGK
                     TTTLEILEGHRPRDAGEVSVLGFDPGRGERAFRERIGIVLQETSVEPYLKVEEAVELF
                     RGYYPAPRPLEEILEVVGLREERGTLVRRLSGGQQRRLDVALGLAGNPDLLFLDEPTT
                     GFDPAARRGAWGMVRNLRTLGTTVVLTTHYLDEAQHLADRVAIVIGGRIVSEGSPEEL
                     TGAGATTIAFVLRADAQPPASLGALRDGERVTIRTEDPVRTLHELTGWALNARVELEG
                     LSVSPRSLEELFLELTAEPEG"
     gene            3346..5976
                     /gene="alaS"
                     /locus_tag="VM638_07235"
     CDS             3346..5976
                     /gene="alaS"
                     /locus_tag="VM638_07235"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HVM12251.1"
                     /translation="MDGSQIRRRFLDFFGERGHTVVPSSSLIPNDPSLLLTTAGMVQF
                     KPYFLGEQDPPYPRAASAQKSFRTTDIELVGHTDRHMTFFEMLGNFSFGDYFKEGAMD
                     HAWALITEGFGIDPARLWVTVYETDDEAEEIWRDHVGVPADRIVRRGKADNFWSMGVA
                     GPCGPCSEIYVDRGPEHGREGGPEVDEDRYLEIWNLVFMQYECDENIEIVGDLPKKNV
                     DTGMGLERMALVLQGKNSAFETDLVRPLLEQAERLADARYGADPSTDISLRILAEHGR
                     ATTFLIADGVTPSNEGRGYVLRRMLRRVVSHARRLGVEKDVTGPLVERTIQLMGEAYP
                     DLVERRAFVLQVAAAEEEQFARTLRQGMERFGDELERTRSSGGGRFSGEAAFLYHDTY
                     GFPIELTVELAKEQGLEVDTSAFERLMEEQRRRAKEAAKGGRDDRALVEIGREAGRSE
                     FLGYERVTAEGRLLGIVTEGARAEAATEGDEVEVVLDRTPFYAEGGGQIGDAGTIRTA
                     SGEVAVEDTVAGPGETIVHRGRVIRGEVHAGEAAEAVVDAPRRAATARSHTGTHVLHW
                     TLRSLLGEHARQAGSLVAPGRLRFDFTHFESVSRDRLEEVEGVANERLIEDEPVRAYE
                     TTMEFARSQGAIALFGEKYGDIVRVVEIGDYSVELCGGTHAVRTGNVSPLVVSSEGSI
                     GSGLRRLEVLVGPDAVAWVNAERRLLEEVAAALGAGDPKQAPDRARKAIARIKELEAQ
                     LGALRKGERGERVEELLTSAVDVAGVALVVADIPGEDVGGLRELALKLRDRLEANGQG
                     AAVLGTTDGAKAQLVAACTKPLLDRGVTAGALLEHAAKAIGGGGGGRPHLAIAGGGNP
                     AGLPEALGGIGERLASLLQG"
     gene            5980..6396
                     /gene="ruvX"
                     /locus_tag="VM638_07240"
     CDS             5980..6396
                     /gene="ruvX"
                     /locus_tag="VM638_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVM12252.1"
                     /translation="MLPAGRVLGLDVGEVRIGVALSDPERRVALPVGTITVAGAPHDL
                     RAVAKLVAEHGVAEVVVGHPISLAGERGQAAVRAEEFADGLRAIVEVPVHLQDERLTT
                     REAERDLRAAGSGRRTRQAVDQAAAALILRAFLERH"
     gene            6437..7474
                     /gene="mltG"
                     /locus_tag="VM638_07245"
     CDS             6437..7474
                     /gene="mltG"
                     /locus_tag="VM638_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVM12253.1"
                     /translation="MAGRHAKRGRGGRIAAIVLLLLVVAGGAGATGLYFWATGTPGGP
                     ARAVPIEVPSGATIAEISELLEEKGVVRSAFAFRLVARLRGAPSIQAGRYELQTNMRP
                     EAALEILDGGPIPEETVTLTIPEGLRVDQVAERVAEVLPVTAKEFLAAAESRRHALPP
                     YLPKRRARSLEGFLFPKTYELPATADADVVIERLLAQFEEEVSGLDWGRAERLGLSPY
                     EIVIVASLIEREARVDEDRRKISSVIYNRIEQGMRLQIDATVQYALPEHKPRLTYADY
                     EYPSPYNTYLNDGLPPTPIASPGLASIEAALRPAKTNYLFYILVDDDGRHAFTESYDE
                     FLNIKNRVQSG"
     gene            7527..>8043
                     /locus_tag="VM638_07250"
     CDS             7527..>8043
                     /locus_tag="VM638_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12254.1"
                     /translation="MDAFDFSGLVARAVAGLDDVRACLLLSRDGLTLGAYPVGGEEQA
                     RAAWSRVEPIGDVERGFLVVGDEVWVFARRGAYACIVVGSGSAKAGVLLDRLEATLRA
                     AEEARMQAGTAPPQPAPAVRRPRTPLHREPKPEPADQVLEEVARVLRMPDDAVGQSPS
                     GPAASAPPPAPP"
BASE COUNT         1167 a         2809 c         2993 g         1074 t
ORIGIN      
        1 ctctcgtcga gggggagctc gtgcagctgt cggcggtctc gtcgggcgtg gcggacgcgc
       61 ggaaggtgat cgagcgcgcc aggggctcga tgttctcgac gatcctcttc gtcgacgagg
      121 tccatcgttg gagcaaggcc cagcaggacg tcctgctgcc ggcggtggaa gaggggaccg
      181 tcacgctgat cggcgcgacg accgagaacc cgtacttctc gctcgtgtcg ccgctcctgt
      241 cccggtgcct cctcctgcgg ctggagcccc tcgacgcgaa ggatctgcgc gacctgctgg
      301 agcgggctct ccgcgacccc gagcggggga tgggcggtga cgatctcgaa gccgacgagg
      361 aggccctgac gttcctcgcc gacgccgccg gcggcgacgc gcggttcgcg ctctccgggc
      421 tcgaggccgc cgcgctcgcg gcgcgcgggg cgggcgagcg gaagatcggc cggtcgctcg
      481 tcgaggacgc ggtccagcgc cgggcgctcg tctacgaccg cgacgagcac tacgacgtca
      541 tctcggcgtt catcaagagc ctccgcggct cggaccccga cgccgggctc ttctacctcg
      601 cgcggatgct ggaggcgggg gaggacgcgc ggttcatcgc ccgccggatg gtgatcctcg
      661 cgtccgagga catcgggccg gccgacccgc agtcgctcct gatcgccaac gccgcggcgc
      721 acgcggtcga gtacgtgggg cttccggagg ccgagctcaa cctcgcccag gcggtcatcc
      781 acctcgcgcg ggcgccgaag agcaacgcgg cctaccgcgc cctcggcgcc gcgaagcgtg
      841 aggcggcgga cccacggcac cgcgtcccgc cgcacctgcg cgacacccac taccgcggcg
      901 cggaagcgct cggccacggc gcgggctacg tgtatccgca cgacgatccc gacggagcgc
      961 gcgaccagcg ctacctcccg gagacggccc gcggcggcta cctcgaggac cgctgaggcc
     1021 gaccatcgga taggctgacg cgcagccgac cgaaggagcg gacgtgaccg gcggagagtg
     1081 ggccctgctg atcgcggcga tcgcgtgggc ggtcctcgtg gtcttcctgg cgatcgtcct
     1141 ggcgaacctc tcgcgcgtcc tcgacagcgc gcggacgatg atcgacgggg tccaccagga
     1201 gacgatcccg ctcctcaccg aggtcacgac ctcggtcaag aaggtcaacc gcgagctcga
     1261 ccgggtcgac ccgctcctcg cctcggcggc gaacatcacg aggtcgatcg agcggctgac
     1321 cgcggtcctc gagcggctcc tcgcctcgcc gctgatcaag gccgcggccg tgggcgccgg
     1381 cgtcacccgc gcgatccggc gtttccgggg agagaagtga ggcgagcgtt ctgggtcgcg
     1441 ctcggcctgg gcgccggcgt gacgagcgcg atcctcgtct cccgctggct ccgtcggcaa
     1501 cgcgagcggt tctccccgtc gaacatcggc gcgcaggtgg gcgagggcgt gcgcgacctc
     1561 cgccagctgc tcggcgtctc cctcgaggag ggacgccgtg cgatgcgcga gcacgaggcc
     1621 gagatccggt cggcgatcga tcccgacgcc tagcggcgcg gctcccagga gaacgtccgc
     1681 agcgcgacca ccaacccggc ggcgccccag gcggcgatcg cgccgaggtg tccccactcg
     1741 aagcccggcc ccgagaggaa cgggtcgtag gcggtctgga gcgcctgcga gaggtgcttc
     1801 accgggaaga ggtcgccgag ggtccggagc cacggcggcg cctggtcgag ccggatgaag
     1861 acgtccgaga cgaacagcag cgggaggatc gtggcattga ccacggcagg ggccgcgtcc
     1921 gcgttgggga tcgccgccgt gatcgcgatc ccgagcgcgc agaacgcggc cgcgccgacc
     1981 gccagcgtca cgaggaccgc cgggagcgaa ccggtcggcg cgcggacgcc gtagaagatc
     2041 atcccgaaca gcgtcacgat cgcgacgagc acgatcgcca gggcgatcac gtgcaggatc
     2101 ttgccggcga ggaaggcgcc cgtggacagc ggcgtcccgc ggatccgctt cagccgtccc
     2161 tggtcacggt cgaaggtcag ccgcatcgcc aggttcgtga aacaggtcgt gatgatcgag
     2221 aaggcggcga tcgccgggac gtagaaggta gaggcggtcg tcgtcccccc ggccacgcgg
     2281 atctccaggt tcccgaagat caggttgaag atcacgagga acatcagcgg gaacgcgaac
     2341 gtgaagacgg ccgaagccgg gttccgccgg aacgcccggt gctcgtagcg caactgccga
     2401 agcgtgcgcc tcatccttcg ggctccgccg tgagctcgag gaagagctcc tcgagcgatc
     2461 ggggcgagac ggagagccct tcgagctcca cgcgcgcgtt cagcgcccat cccgtgagct
     2521 cgtgcagcgt ccgaacgggg tcctcggtcc ggatcgtcac ccgctcgccg tcgcgaagag
     2581 cgccgagcga cgccggcggc tgggcgtcgg cccgcaggac gaaggcgatc gtcgtcgctc
     2641 ccgcgccggt gagctcctcc ggcgaccctt cgctcacgat ccgtccgccg atcacgatcg
     2701 cgacgcgatc ggcgaggtgc tgggcctcgt cgaggtagtg cgtcgtgagc acgacggtgg
     2761 tgccgagcgt gcggaggttc cggaccatgc cccaggcgcc acggcgcgcg gcgggatcga
     2821 acccggtcgt cggctcgtcc aggaagagga ggtcggggtt gccggcgaga ccgagcgcga
     2881 cgtcgagccg ccgctgctgg ccgcccgaga ggcgtcgcac gagcgtccca cgctcctcgc
     2941 gtagtccgac gacctcgagg atctcctcga gcggcctcgg cgcggggtag tagccgcgga
     3001 agagctcgac cgcctcctcg accttcaggt agggctcgac gctcgtctcc tgcaggacga
     3061 tcccgatccg ctcgcggaag gcccgttcac cgcgcccggg gtcgaagccg aggacgctca
     3121 cctcgcccgc gtcccgcggg cggtggccct cgaggatctc gagggtcgtg gtcttcccgg
     3181 cgccgttcgg cccgaggagc gcgaagatct cgccccggcg gatcgccagg tcgaggcccc
     3241 ggaccgcctc gatctgcccg taccgcttgc ggaggtcccg gacctcgatc gccagctccg
     3301 acatccgggt catcctacgg gcgcccgtcg atagactgag gcccgatgga tgggtctcag
     3361 atccggcgcc gcttcctcga cttcttcggc gagcgggggc acacggtcgt gccgtcgtcc
     3421 tcgctgatcc cgaacgaccc gagcctgctg ctcaccaccg cggggatggt gcagttcaag
     3481 ccgtacttcc tcggggagca ggatccgccg tacccgcggg cggcctccgc gcagaagtcc
     3541 ttccgcacga ccgacatcga gctcgtgggt cacaccgacc gccacatgac gttcttcgag
     3601 atgctcggga acttctcgtt cggcgactac ttcaaggaag gcgcgatgga ccacgcctgg
     3661 gcgctgatca ccgagggatt cggcatcgac ccggcgcgcc tgtgggtcac ggtctacgag
     3721 accgacgacg aggccgagga gatctggcgc gaccacgtcg gcgttccggc cgaccgcatc
     3781 gtccgccgcg gaaaggccga caacttctgg tcgatggggg tcgccggtcc ctgcgggccc
     3841 tgctcggaga tctacgtcga ccgggggccg gagcacggcc gcgagggcgg ccccgaggtc
     3901 gacgaggatc gatacctcga gatctggaac ctcgtcttca tgcagtacga gtgcgacgag
     3961 aacatcgaga tcgtcggcga ccttccgaag aagaacgtcg ataccgggat gggcctcgag
     4021 cggatggcgc tcgtccttca ggggaagaac tcggcgttcg agacggacct cgtccggccg
     4081 ctcctcgagc aggcggagcg gctcgccgac gctcgctacg gcgccgaccc gtccaccgac
     4141 atctcgctcc ggatcctcgc cgagcacggc cgcgccacga ccttcctgat cgccgacggc
     4201 gtgaccccgt cgaacgaggg tcgcggttac gtcctgcgcc ggatgctgcg acgcgtcgtc
     4261 tcgcacgccc ggcggctcgg cgtcgagaag gacgtcaccg gcccgctcgt cgagcggacg
     4321 atccagctga tgggggaggc gtaccccgac ctcgtggagc gccgggcctt cgtcctgcag
     4381 gtcgcggcgg cggaggagga gcagttcgcg cggacgcttc gccaggggat ggagcgcttc
     4441 ggggacgagc tcgagcgcac gcgctcgtcc ggcggtggcc gcttctccgg cgaggcggcg
     4501 ttcctctacc acgacaccta cggcttcccg atcgagctca ccgtggagct cgcgaaggag
     4561 cagggcctcg aggtcgacac ctccgcgttc gaacggctga tggaggagca gcgccggcgg
     4621 gcgaaggagg ccgcgaaggg tgggcgggac gaccgcgcgc tggtcgagat cggacgagag
     4681 gcgggccgca gcgagttcct cgggtatgag cgcgtcacgg cggaagggcg cctgctcggg
     4741 atcgtcacgg agggagcccg tgcggaggcc gccacggaag gcgatgaggt cgaggtcgtc
     4801 ctcgaccgga caccgttcta cgccgagggc ggcggccaga tcggcgatgc ggggacgatc
     4861 cggaccgcga gcggcgaggt cgccgtcgag gacaccgtcg ccggaccggg ggagacgatc
     4921 gtccaccggg ggcgcgtgat ccgcggcgag gtccacgcgg gcgaggcggc cgaggcggtg
     4981 gtcgatgccc cccgtcgtgc cgcgacggcc cgctcccata ccggcacgca cgtcctgcac
     5041 tggacgctcc ggagcctcct gggcgagcac gcgcgccagg cgggctcgct cgtcgcgccc
     5101 gggcgactgc gcttcgactt cacgcacttc gagtcggtct cgcgtgaccg cctcgaggag
     5161 gtggaggggg tggcgaacga gcgcctgatc gaggacgagc cggtccgcgc gtacgagacg
     5221 acgatggagt tcgcgcgcag tcagggcgcg atcgccctct tcggagagaa gtacggggac
     5281 atcgtccgcg tcgtggagat cggggactac tcggtggagc tgtgtggggg gacgcacgcg
     5341 gtgcggacag gcaacgtctc gcccctcgtc gtctcgtcgg agggcagcat cggatccggg
     5401 ttgcgccggc tggaagtgct ggtcgggccg gacgccgtcg cgtgggtcaa cgcggagcgc
     5461 cgcctgctgg aggaggtcgc ggcggccctc ggggccggcg acccgaagca ggcgccggac
     5521 cgggcgcgga aggcgatcgc ccggatcaag gagctcgagg cccagctcgg ggccctcagg
     5581 aagggcgagc ggggagaacg ggtcgaagag ctgctcacct cggccgtcga cgtcgccggg
     5641 gtggccctcg tcgtcgccga catccccggc gaagacgtcg gcggcctccg ggagctggcc
     5701 ctgaagctcc gggaccgcct cgaagcgaac gggcaagggg cggcggtgct cggcaccacc
     5761 gatggcgcga aggcccagct cgtcgcggcc tgcacgaaac cgctgctcga ccgcggcgtg
     5821 accgcgggcg ccctcctcga gcacgccgcg aaggcgatcg gcggcggcgg tggcgggcgg
     5881 ccgcacctgg cgatcgcggg cggcggcaac ccggccgggc tgccggaggc gctcggcggg
     5941 atcggggagc gcctcgcctc gctcctgcag ggctgatcgg tgctcccggc cggacgtgtt
     6001 ctcggtctcg acgtcggtga ggttcgcatc ggggtcgcgc tcagtgatcc ggagcgacgc
     6061 gtcgcgctcc ccgtggggac gatcacggtc gccggcgcgc cgcacgacct ccgcgccgtc
     6121 gcgaagctcg tcgccgagca cggggtcgcc gaggtcgtgg tcggccaccc gatctcgctc
     6181 gcaggggagc ggggccaggc ggccgtgcgg gccgaggagt tcgccgatgg gctccgcgcg
     6241 atcgtcgagg tcccggtgca cctgcaggac gagcggctga cgacgcgcga ggccgagcgg
     6301 gacctgcgcg cggccgggag cggccggcgg acgcgccagg ccgtcgacca ggccgcggcg
     6361 gccctgatcc tccgggcgtt cctcgagcgg cactgagggc gacgccgctc ccgcggcggc
     6421 gatatcctca cgccccatgg ccgggcgaca cgcgaagcga ggtcgaggcg gcaggatcgc
     6481 ggcgatcgtg ctgctgctgc tggtcgtcgc cggtggcgcc ggcgcgaccg gcctctattt
     6541 ctgggccacc gggacccccg gggggccggc ccgggcggtc ccgatcgagg tcccgtccgg
     6601 ggcgacgatc gctgagatct ccgagctgct cgaggagaag ggagtcgtcc ggtcggcctt
     6661 cgccttccgt ctggtcgcgc ggctccgagg cgcgccctcg atccaggcgg ggaggtacga
     6721 gctccagacg aacatgcggc cggaggcggc gctcgagatc ctggacggcg ggccgatccc
     6781 ggaagagacg gtgaccctca cgatccccga ggggctgcgc gtcgaccagg tcgccgagcg
     6841 ggtcgcggag gtcctgcccg tcacggccaa ggagttcctg gcggccgccg agagccggag
     6901 gcacgcgctc cctccgtacc tgccgaagcg gagggcgcgt tcgctcgagg ggttcctctt
     6961 cccgaagacc tacgagctgc cggccacggc cgacgccgat gtcgtgatcg agcgcctcct
     7021 cgcgcagttc gaggaggagg tttcgggcct cgactggggg cgggcggagc gtctcggcct
     7081 cagcccgtac gagatcgtga tcgtcgcctc gctgatcgag cgggaagcgc gcgtcgacga
     7141 ggaccggcgc aagatctcct cggtcatcta caaccggatc gagcagggga tgcgtctgca
     7201 gatcgacgcc acggtccagt acgcgctgcc agagcacaag ccgcgcctga cctacgcgga
     7261 ctacgagtac ccgagcccct acaacacgta cctgaacgac ggcctcccgc cgacgccgat
     7321 cgcgagcccc gggctcgcgt cgatcgaggc cgcgctccga ccggcgaaga cgaactacct
     7381 cttctacatc ctcgtcgacg acgacggccg ccacgccttc accgagtcct acgacgagtt
     7441 cctgaacatc aagaaccggg ttcagagcgg ctgaccccgt tcctcaaggt cctaggccgg
     7501 ccggccgaca accgcaagag gaacacatgg acgctttcga cttctctggt ctggtggcga
     7561 gggccgtggc aggcctcgac gacgtgcggg cctgcctcct gctctcccgc gacggactga
     7621 cgctcggcgc ctacccggtg ggcggcgagg agcaggcgcg ggccgcctgg agccgggtcg
     7681 agccgatcgg cgacgtggaa cgcgggttcc tcgtcgttgg cgatgaggtc tgggtcttcg
     7741 ccaggcgggg cgcttacgcc tgcatcgtcg tcgggagcgg gtcggcgaag gccggggtgc
     7801 tcctcgaccg tctcgaggcc acgctccgtg cggccgaaga ggcgcggatg caggcgggga
     7861 ccgcgccgcc gcagccggcg ccagccgtgc gacggccgcg cacgccgctg caccgcgagc
     7921 cgaagcccga gccggccgat caggtcctcg aggaggttgc gcgcgtgctg cgcatgcccg
     7981 acgacgccgt cggtcagtcg ccgtcggggc cggccgcatc ggcgcctccg ccggctccac
     8041 ccc
//
LOCUS       DATLGG010000253         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_214185, whole genome shotgun sequence.
ACCESSION   DATLGG010000253 DATLGG010000000
VERSION     DATLGG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214185"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VM638_07255"
     CDS             complement(<1..265)
                     /locus_tag="VM638_07255"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HVM12255.1"
                     /translation="MTTRIEKTEEEWRKELSPEEYHVLREAGTERPFTGEYWDHHEEG
                     DYRCRACGAKIFDSGTKFDSGTGWPSFTEPMNREHVELVPDTSH"
     gene            444..2090
                     /locus_tag="VM638_07260"
     CDS             444..2090
                     /locus_tag="VM638_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12256.1"
                     /translation="MRSSLAPVAEVFRNPDLRRLLLGSSGYFTGDWAYTIALAVFAYR
                     AGGPAAVGLVGLIRMVPAAVIAPFGSMLADRYRRERVLTLVYLSCAVIGALSATAYFL
                     TSSTILIFVLAGLISAVSAVLRPAHWALVPLLATNPRQMAASNAASSMVEGVSTFVGP
                     AIGAALLVTGSAGIVFAATAAMFAWSAFITARITSDITAKIKPRTENEPPLRVAAEGF
                     RILLREPGPRLLVALFSAQTLVRGLLNVLLVVASVELLGLGESGVGILTSAFGVGGIV
                     GTLATLWLVGRRRLAGPFGLGLALWGLPIALVGFFPNPAAALVLLALPGVGNAIGDVA
                     GYTLVQRLTKNDVLARVFGVLEGSVTVTLGIGYVLAAPLLAVAGTRGALIITGLLLPV
                     LVLASLRALRRLDRVCEVPERELSLLGQIPLFSPLPVVTREQLASALDREEHPAGRVL
                     VREGQRGDRFYIVAEGEVLVSAAGREAATLGPGDYFGEIALLRDVPRTATVSTTTSVV
                     TYALNREEFIGAVTGHVATEDEAETTVAARLAGLQGMRES"
     gene            complement(2087..>3164)
                     /locus_tag="VM638_07265"
     CDS             complement(2087..>3164)
                     /locus_tag="VM638_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVM12257.1"
                     /translation="LFLGLSFLVEWVIDAWSRLPRVDHAIVLLILVVIGAFGFLEGVA
                     LGLALAVVLFVVSYSRTEVVKHALTGETFTSNVDRSPREREILRSAGAGVQVFELQGF
                     LFFGTANRLLDRIRARAGDPSAPPLRSLILDFRRVSGLDSSAVLSFTKAAQLAGRQGF
                     RIALAGVPAAARRQLERGGVEQEGQVAFFEDTDRALQWHEDRILEEAELEADATATFR
                     ERLAAEMPDEDVETLLGYVERMEVPAGQELISQGGSSGDVFFLERGRLTARLRQPDGG
                     WTRLRTMGSGTVVGEMAMYLGIPRSASVVAEEASVLYRLPDGAIGLMEERHPRVAAGL
                     HRFFARLLSERLADTLRSLSALLD"
BASE COUNT          416 a         1175 c         1090 g          483 t
ORIGIN      
        1 cgtggctcgt gtcgggaacg agctcaacgt gctcgcggtt catcggctcc gtgaagctcg
       61 gccatccggt gccggagtcg aacttcgtcc cggagtcgaa gatcttcgct ccgcacgcgc
      121 gacagcggta gtcgccctcc tcgtggtggt cccagtactc cccggtgaag gggcgctcgg
      181 tgccggcctc ccgcaacacg tggtactcct ccgggctgag ctccttgcgc cactcctcct
      241 cggtcttctc gatcctcgtc gtcacggtcc ctcctcgggt cggatgagcc ttccaacgcc
      301 cgcgcggcat cgactgttcc cgccagccta gcccgccgcc ggggggcgaa gggtcgggaa
      361 ccccccgaac ggactattgg cgcgccgttc ccgtgtgcca tcctgggggc gtggaaggca
      421 cgcgcacgcg gccgagcggc cggatgcgat cttcgctcgc tcccgtggcg gaggtcttcc
      481 ggaacccgga cctccgccgg ctcctgctcg ggtcgagcgg ctacttcacc ggagattggg
      541 cgtacacgat cgcgctcgcc gtcttcgcct atcgcgcggg tggcccggcc gcggtcggtc
      601 tcgtcggtct gatccgcatg gttccggcgg cggtgatcgc gcccttcggg tcgatgctcg
      661 cggatcgcta ccgacgggag cgcgtgctga cgctcgtgta cctctcctgc gccgtgatcg
      721 gggcgctctc cgcgacggcc tacttcctca cctcatcgac catcctgatc ttcgtcctgg
      781 cgggcctcat cagcgccgtg tccgcggtgc tccgcccggc gcactgggcg ctcgtgcccc
      841 tgctcgcgac gaacccgcga cagatggccg cctcgaacgc ggcatcgagc atggtcgagg
      901 gcgtcagcac gttcgtcggg cccgcgatcg gcgcggccct gctcgtcacg ggcagcgccg
      961 gcatcgtctt cgcggccacc gcggcgatgt tcgcctggtc ggccttcatc acggccagga
     1021 tcacgagcga catcacggcg aagatcaagc cgaggacgga gaacgagccc ccgctgcgcg
     1081 tcgcggccga ggggttccgg atcctgctac gggaaccggg cccgcgcctt ctcgtcgccc
     1141 tcttctccgc ccagacgctc gtccgcggtc tgctgaacgt gctgctcgtc gtcgcctcgg
     1201 tggagctgct cgggctcggg gaatccggtg tcgggatcct cacgtcggcg ttcggcgtcg
     1261 gggggatcgt gggaacgctg gcgacgctct ggctggtcgg ccggcggcgc ctggcggggc
     1321 cgttcggact cgggctggcg ctgtggggac tgccgatcgc cctcgtcggc ttcttcccga
     1381 atcccgccgc cgcgctcgtc ctcctcgccc tgcccggggt ggggaacgcg atcggggacg
     1441 tcgccggcta cacgctcgtc cagcgcctga cgaagaacga cgtcctggcc cgcgtgttcg
     1501 gcgtcctcga gggctccgtc accgtgacgc tcgggatcgg gtacgtcctc gcggcgccgc
     1561 tcctggcggt cgccggcacg cggggcgcgc tcatcatcac gggcctgctc ctgcccgtcc
     1621 tcgtgctcgc ctcgttgcgc gcgctgcggc gcctcgaccg cgtgtgcgag gtcccggagc
     1681 gggagctgtc gctgctcggc cagatcccgc tgttctcccc gctgccggtc gtgacccgcg
     1741 aacagctggc gtcggcgctc gaccgcgagg agcaccccgc cggccgcgtg ctcgtgcgcg
     1801 aaggtcaacg cggggaccgc ttctacatcg tcgccgaggg cgaggtcctc gtgagcgccg
     1861 cggggcggga ggccgcgacc ctcgggccgg gcgactactt cggggagatc gcgctgctgc
     1921 gcgacgtccc ccggaccgcc accgtcagca cgacgacgtc ggtggtgacc tacgcactca
     1981 accgcgagga attcatcggt gccgtcacgg gtcacgtggc cacggaggac gaagccgaga
     2041 cgacggtcgc ggcgcgactc gccgggctcc aggggatgcg cgagagctag tcgagcaggg
     2101 ccgagagcga ccggagggtg tcggcgagcc gctccgagag gagccgggcg aagaaccgat
     2161 ggaggccggc cgccacccgc ggatggcgct cctccatcag cccgatcgct ccgtccggga
     2221 gccggtagag cacgctcgcc tcctcggcca cgaccgaagc cgagcggggg atcccgaggt
     2281 acatcgccat ctcccccacc accgttccgc tgcccatcgt gcgcagacgc gtccagccgc
     2341 cgtcgggttg ccgcaggcgc gccgtgagcc ggccccgttc gaggaagaag acgtcgccgg
     2401 aggagccgcc ctggctgatc agctcctgtc ccgccggcac ctccatccgc tccacgtagc
     2461 cgagcagcgt ctcgacgtcc tcgtcgggca tctcggcggc gagccgctcg cggaacgtcg
     2521 cggtcgcgtc cgcctccagc tccgcctcct ccaggatccg gtcctcgtgc cactggaggg
     2581 cgcggtcggt gtcctcgaag aacgccacct gcccctcctg ctcgacccca cctcgctcga
     2641 gctgccgccg agcggcggcc ggcacgccgg cgagcgcgat ccggaacccc tgccgtccgg
     2701 cgagctgcgc cgccttcgtg aagctcaaga ccgccgacga gtcgagcccc gagacgcggc
     2761 ggaagtcgag gatgagggac cgcagcggcg gagccgaggg atcgcccgct cgtgcccgga
     2821 tccggtcgag cagccggttc gccgtcccga agaacaggaa cccctgaagc tcgaagacct
     2881 ggaccccggc tcccgccgaa cggaggatct ctcgctcgcg aggcgaccgg tcgacgttgc
     2941 tcgtgaaggt ctcgccggtc agcgcgtgct tgaccacctc ggtgcggctg tagctgacga
     3001 cgaacaggac gaccgcaagg gccagcccca gggccacgcc ctcgaggaat ccgaaggcgc
     3061 cgatgaccac caggatcaac agcacgatgg cgtggtccac gcgcggcagc ctcgaccacg
     3121 catcgatgac ccactcgacc aagaaggaca gcccgaggaa gagc
//
LOCUS       DATLGG010000254         7504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_62322, whole genome shotgun sequence.
ACCESSION   DATLGG010000254 DATLGG010000000
VERSION     DATLGG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62322"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1608
                     /locus_tag="VM638_07270"
     CDS             211..1608
                     /locus_tag="VM638_07270"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HVM12258.1"
                     /translation="MDIHESLIDVIGDTPLLRLGRIAADHEATVVAKLEMLNPGGSVK
                     DRIGIRMIDAAERAGLLKPGGTIVEPTSGNTGVGLAMAAALKGYRCIFTMPDKMSQEK
                     ISLLRAYGAEVVIAPTAVPPDHPDSYYRVADRLSREIPGAFQPNQYFNQENPRTHYET
                     TGPEIWRQTAGRITHFVCGVGTGGTITGTARYLKEQNPDVVIVGADPEGSVYTGSQAR
                     PYLVEGIGEDFFPETFDRSVVDRWVTVSDRDSFLTARRVTQEEGLLVGGSGGTAVFAA
                     LEIARDAGPDAVVVVIIPDSGKSYLSKLYNDAWMSEYGFLERPGRRARIADVLGEKRR
                     AESEIPDLVIVPQDETVGRAIDVLQEFGISQLPVAKEAEAGDLAELVGSIQERSLLDR
                     VFRDPDAINRTIGEVMDPPLPHVQTTAGIDELFADLSQGAEALVVAEGAHPVGVVTRA
                     DLLEFLAHQNRRRGT"
     gene            complement(1605..2483)
                     /locus_tag="VM638_07275"
     CDS             complement(1605..2483)
                     /locus_tag="VM638_07275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HVM12259.1"
                     /translation="MRALLAPRDPSPAAVFMAAASCVVVVLAATAGRLGRLPPVRSLA
                     AAPVLGLLLAAGAHLALVLPLAIVYPPAQDLVDVIVGRPGGDELDARLIVVLLVTAGV
                     LAPLTEEPAKAVSGGFARAGSRSAAFLAGATAGAGFSIVEHAFYSLPVAGYGETWVQV
                     LLLRSASAAMHPLASGLVFLGWWEARRAGTKAPLIRGLLLGIGIHAVWNVTAVVAEGF
                     ATASRAGAFPRSVEAAALTYAGAIAILIAVAMWRTVTSLRAGSAPERPSGRAWAALVA
                     GLAGPLALLPVVLPRL"
     gene            complement(2575..3714)
                     /locus_tag="VM638_07280"
     CDS             complement(2575..3714)
                     /locus_tag="VM638_07280"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HVM12260.1"
                     /translation="MRFETYAIHAGQDPEPATGAAVVPIYQTSTYVQEAVGRHKGFEY
                     SRTDNPTRHALEQCLAALEAAEAAVAFGSGMAAIATLALTLEQGSRVLVADDVYGGTY
                     RLFARVLPQQGIDFATVDMTDLAAVEAAAAEGCDLIVVESPGNPMLKVLDLEALARVA
                     HDAGALLAVDNTFATMFLQRPLQHGADVVLYSATKYLGGHSDLVMGSVATNDGELAER
                     LRFLQNAVGAIPGPFDSWLLLRGLKTLAVRMRAHCAGAARVAEWLARHPAVERVHYPG
                     LPDSPGHDLARRQMSASGEPLFGGMVSFETTGEEEALRVCEGTRLFFLAESLGGVESL
                     IEHPGRMTHASLAGSGMEVSPALVRLSVGIEHPDDLIADLERALG"
     gene            3778..4623
                     /locus_tag="VM638_07285"
     CDS             3778..4623
                     /locus_tag="VM638_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HVM12261.1"
                     /translation="MARRNAKIRRSIEGTSGTGSRMDNQHEGHIVVVGQWREGQGYVP
                     APYARAVAAAGGRPVIFSTFDLPSHQLPDGISHVERVDPDDATAIDGAAGLVLPGGGD
                     IDPRWYGREPHPATHNVNHRRDRYERTLLEAALERDVPVLAICHGMQMLNVHLGGTLD
                     QNLADEPGRLRHDHEAPTPEPVHDVQVAPNTLLAEAVGGGRLPVNSHHHQGLDVLALP
                     LAGVAWAEDGVLEAVVSREHSWVVGVQWHPEVMAAIDPRQGRLFEGLVRAAAAYRGEV
                     TPAAS"
     gene            complement(4630..6015)
                     /locus_tag="VM638_07290"
     CDS             complement(4630..6015)
                     /locus_tag="VM638_07290"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HVM12262.1"
                     /translation="MTERRREAYFAAGVGLLLAAGLVLDLTSGDVPPAEAEAGGRGGF
                     EAHSVFCPGAVAGGAADVDLVIVAPEGDRLAVGIEPQEGETAEVPREGALIYDVSGSE
                     PADIVGYGAPAGVAVLTSTERPGAGAGAAGCARTAGANWYFAAGSASLEADERILLYN
                     PFPDEAVVRLSLFTPEGEQTKASLADVAVPANAFTVVPLNEKVGVENTLAVHVAAERG
                     RVVAWRQTFVSGRDRVPGMAFSLGAARPSALWHFPDGAVGGGSSERISILNPSQEEAV
                     VAISLAVGRDEVVAPPDLMELAVPPRSQLVVGLGAHVETGDEPVAVAASVRSVNDVPV
                     VAEREVVVDGAEGRGIAAEVGSSADALRWLVPPAARNSSADAIAILNPATEPATVSLR
                     LLDRNGVEIAAPGLEAVEIGPGGRVRIPVARWTAGKPVLALVDATVPVVVERSAIAAG
                     DAVALMGLPLR"
     gene            complement(6012..>7504)
                     /locus_tag="VM638_07295"
     CDS             complement(6012..>7504)
                     /locus_tag="VM638_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12263.1"
                     /translation="RVALGAAVSAAFVPGSLLLYALVAILVAIARAVLDRGGRALQGL
                     VSAGIGLALAWVLLLPWSTTWFAPGGVMNRLLGDDTAPLFAASFRGHGMASTLLGQTP
                     DGPPLFGLALPLLGLVAVLVGEGSRRRMALALWSVVVTSGIVVELISTGVLRPIVAAP
                     TEAGVLASLAFAGLAGLAIGAFRLDLRRKALGWQHALTIGVLGISLFLAVTGLGPTTL
                     AGGWDPGRTSGGIEAPVVQQVSSLLDAEAQQIGEFRALWVGDTWLPDEPSALRPPGEH
                     FITGARSSLLTDLFQPATGPGETRLRRVIWSIESGETDRGGRLLGAFNVHFVIVERGP
                     DARGWLAQRDLAVIRTEPTYHVLRNQLELPRAAVFDELPAYARALDEGPGALAEVGEE
                     EVEPVAELRSEGAAGPFRGRAAGPGVVFLAQARHPQWEARAGEERLGRVDSGWGNAFA
                     LPAGDATVEVWFASSPLRYVWYGSLVFMWLVVIGAAAPRRARRRRT"
BASE COUNT         1183 a         2779 c         2611 g          931 t
ORIGIN      
        1 cgcggaagtc gcgccctccg gcgcgtcctc cggtctcagc tcgtccatgg ctctcctatg
       61 ggtgtgggtg ccgcaagggt aaaccgttcg tgcacgactt cacaaagggg tagaccgtcg
      121 cctctatgac gccgcgccgc cgcgggtggt tgcagtcgcc ctcccgacac cctccttgca
      181 ggccgggcgt cggtggtgaa cgatcagccg atggacatcc acgagtcgct catcgacgtc
      241 atcggcgata cgccgctcct tcgtctcggc cggatcgccg cggatcacga ggcgaccgtc
      301 gtcgccaagc tcgagatgct gaacccgggc gggagcgtga aggatcgtat cggcatccgc
      361 atgatcgacg ccgccgagcg ggcgggtctg ttgaaaccgg gcgggacgat cgtcgagccg
      421 acctccggga acaccggcgt cgggctggcc atggccgccg cgctcaaggg gtaccgctgc
      481 atcttcacga tgcccgacaa gatgagtcag gagaagatct cgctcctgcg tgcctacggc
      541 gcggaggtcg tgatcgcgcc cacggcggtg ccgccggacc atcccgactc gtactaccgc
      601 gtggccgacc gcctgagccg cgagatcccc ggcgcgttcc agccgaacca gtacttcaac
      661 caggagaacc cgcggacgca ctacgagacg acgggtcccg agatctggcg ccagaccgcc
      721 ggccgcatca cgcacttcgt ctgcggtgtc ggaacgggcg ggacgatcac cgggacggcc
      781 cgctacctga aagagcagaa ccccgacgtc gtcatcgtgg gagccgaccc cgaaggctcg
      841 gtctacaccg gaagccaggc ccgtccctac ctcgtcgagg gcatcggtga ggacttcttc
      901 cccgagacgt tcgaccgctc ggtggtcgat cgttgggtga cggtttcgga tcgagactcc
      961 ttcctcacgg cgcgccgggt cacgcaggag gagggcctgc tcgtcggcgg ctccgggggc
     1021 accgccgtat tcgcggcgct cgagatcgcg cgggacgcgg ggcccgacgc ggtggtcgtg
     1081 gtgatcatcc cggactccgg caagtcgtac ctgtcgaagc tctacaacga cgcgtggatg
     1141 agcgagtacg gcttcctcga gcggccgggg aggcgcgccc gcatcgccga cgtcctgggg
     1201 gagaagcggc gcgccgagtc ggagataccc gacctggtca tcgtgccgca ggacgaaacg
     1261 gtcggccggg ccatcgacgt cctccaggag ttcggcatct cgcagctccc cgtggccaaa
     1321 gaagcggagg cgggggacct ggccgagctc gtcggctcga tccaggagcg ttccctgctc
     1381 gaccgcgtct tccgcgaccc cgacgcgatc aaccggacca tcggtgaggt gatggacccg
     1441 ccgcttcccc acgtgcagac gacggccggc atcgacgagc tcttcgccga cctttcacag
     1501 ggtgcggagg cgctggtcgt ggccgagggc gcgcatcccg ttggggtggt gacgcgcgcc
     1561 gacctgctcg agttcctcgc ccaccagaac cgccggcgcg gaacctagag ccgggggagg
     1621 acgaccggca ggagcgcgag cggcccggcg agaccggcga cgagagccgc ccacgcgcga
     1681 cccgaaggcc tctccggagc ggagcccgcc cgcagcgagg taacggtccg ccacatcgcc
     1741 accgcgatga ggatcgcgat cgcacccgca taggtaagag ccgccgcctc caccgaccgc
     1801 gggaacgctc cggcgcgcga cgcggtcgcg aagccctccg cgaccacggc ggtcacgttc
     1861 cacaccgcat ggatcccgat gcccaggagc aggcctcgga tcagcggcgc cttcgtcccg
     1921 gcgcgccgtg cctcccacca gccgaggaag acgagcccgc tcgcgagggg gtgcatcgcc
     1981 gccgaggcgc tgcgcagcag caggacctgg acccacgtct cgccgtatcc ggcgacgggt
     2041 agcgagtaga aggcgtgctc gacgatagag aagccggcgc cggccgtcgc gccggcgagg
     2101 aacgcggcgc tccgagaacc ggcgcgggcg aatccccccg acacggcctt cgccggctcc
     2161 tcggtgagcg gcgccaggac gccggcggtg acgagcagca cgacgatcag ccgcgcgtcg
     2221 agctcgtctc ccccgggtcg tccgacgatc acgtcgacga ggtcctgcgc cggcgggtag
     2281 acgatcgcga gcggcagaac gagggcgagg tgcgcgccgg ccgcgagcag cagcccgaga
     2341 acgggcgcgg ccgccagcga ccgcaccggg ggcaggcgcc cgagacgtcc ggcggttgcg
     2401 gcgagcacga cgacgacaca cgacgcggcc gccatgaaca ccgcggccgg agacggatcg
     2461 cgcggtgcca gcagggcgcg cacgagcccc gcggtagcga gcgctcctcc tcccgcggcc
     2521 gccgcgtagg caggccacgt tcggatgctt cgctccggat gcgccacggg cgcctcagcc
     2581 gagcgcgcgc tcgaggtccg ctatgaggtc gtccgggtgc tcgatgccga ccgacagccg
     2641 caccagcgcg ggcgacacct ccatgccgga cccggcgagg ctcgcgtgcg tcatgcggcc
     2701 cggatgctcg atcaaggact cgacgccccc gagggactcg gccaggaaga acaagcgggt
     2761 cccctcgcat acccgcagag cctcctcttc gccggtcgtc tcgaacgaaa ccatcccgcc
     2821 gaagagaggc tcgccggacg cgctcatctg ccggcgcgcg agatcgtgtc ccggcgagtc
     2881 gggcaacccg ggatagtgaa cgcgctcgac cgccgggtgc cgcgcgagcc actcggcgac
     2941 gcgggcggcg ccggcgcagt gggcacgcat cctcaccgcg agcgtcttga ggccccgcag
     3001 caggagccac gagtcgaacg ggcccgggat cgccccgacc gcgttctgca ggaaacggag
     3061 cctctcggcg agctcgccgt cgttcgtggc gacggagccc atcacgaggt cggagtgtcc
     3121 gccgaggtac ttcgtcgcgg agtagaggac gacatcggcc ccgtgctgaa gcggcctctg
     3181 gaggaacatc gtggcgaagg tgttgtcgac ggcgaggagc gcgccggcat cgtgcgcaac
     3241 acgggccagc gcctcgaggt cgagcacctt cagcatcggg ttgcccggcg actccaccac
     3301 gatgagatcg catccctccg ccgccgcggc ctcgaccgcg gcgaggtcgg tcatatcgac
     3361 ggtggcgaag tcgatcccct gctgcggcag aacccgcgcg aacagccggt aggtgccgcc
     3421 gtagacgtcg tcggcgacga ggacgcggga cccctgctcg agggtcaagg cgagcgtggc
     3481 tatcgcggcc ataccggagc cgaacgcgac cgcggcctcg gcggcctcga gggccgcgag
     3541 gcactgctcc agcgcgtgcc gggtcgggtt gtcggtgcgc gagtactcga agcccttgtg
     3601 ccgccccacc gcctcctgga cgtacgtcga ggtctggtag atcggcacga cggcggcgcc
     3661 ggtcgccggc tccgggtcct gacctgcgtg gattgcgtat gtctcgaatc gcatcgtgtc
     3721 gctcatatgg atgaaatgcg tcctttagtc gcgggcgtcg tggacgatgc cgatagcatg
     3781 gcacggagga acgcgaagat ccgccgaagc atcgagggga cgtccgggac cgggagcagg
     3841 atggataacc aacacgaggg tcatatcgtc gtcgtcggtc agtggcgcga gggccagggt
     3901 tacgtcccag ctccgtacgc gcgggcggtg gcggcggccg gcggacggcc ggtgatcttc
     3961 tcgaccttcg acctgccctc gcaccagctg cccgacggga tctcccatgt cgagcgagtg
     4021 gacccagacg acgccacggc gatcgacggc gcggccgggc tcgtcctgcc cggcggaggc
     4081 gacatcgatc cccgctggta cggccgcgag ccgcacccgg cgacgcacaa cgtgaaccac
     4141 cggcgcgatc gttacgagcg gaccctgctc gaggccgcgc tagagcggga cgtccccgtg
     4201 cttgcgatct gtcacggcat gcagatgctc aacgtgcacc tgggcggcac gctcgatcag
     4261 aacctcgccg acgagcccgg tcgcttgcgc cacgatcacg aggcgcccac gcccgagccg
     4321 gtccacgacg tccaggtcgc acccaacacg ctgctcgccg aggcggtcgg gggcggccgg
     4381 ctgcccgtca actcgcacca ccaccagggc ctcgacgtgc tcgcgcttcc gctcgccggc
     4441 gtcgcctggg cggaggacgg cgtgctcgag gcagtggtct cgcgcgagca ttcctgggtc
     4501 gtgggcgtgc agtggcatcc cgaggtcatg gcggcgatcg atccccgcca ggggcgactt
     4561 ttcgagggac tcgtgagggc cgccgccgcc taccgcggcg aggtcacccc ggcagcctcg
     4621 tagcctctct cagcggagcg gcagccccat gagagcgacg gcgtcgccgg ccgcgatcgc
     4681 agagcgctcc acgaccaccg ggaccgtcgc atccaccagc gcgagcaccg gcttccccgc
     4741 ggtccagcgg gcgaccggga tgcgcacgcg cccgccgggg ccgatctcca ccgcctccaa
     4801 cccgggagcg gcgatctcca cgccgttgcg gtcgaggaga cgcaacgaga cggtggcggg
     4861 ctccgttgcc gggttcagga tggcgatcgc gtccgccgag gaattgcggg ccgccggcgg
     4921 gacgagccac cgcagcgcgt ccgcggagga cccgacctcg gccgctatgc ctctgccttc
     4981 agcgccgtcg acgacgacct ccctctcggc tacgacgggc acgtcgttga ccgagcggac
     5041 gctggccgcc accgccaccg gctcgtcgcc cgtctccacg tgcgcgccga gacccaccac
     5101 gagctgcgac cgtggcggaa ccgccagctc catgagatcg ggcggcgcta cgacctcgtc
     5161 ccgtccgacc gcgagcgaga tcgccaccac cgcctcttcc tggctcgggt tgaggatcga
     5221 gatgcgctcc gacgacccgc cgcccaccgc cccgtccggg aagtgccaca aggccgacgg
     5281 ccgcgccgcc cccagcgaga aggccatgcc cggaacgcgg tcgcggcccg atacgaacgt
     5341 ctgcctccat gccacgaccc gcccgcgttc ggcggcgacg tgcacggcga gggtgttctc
     5401 cacccccacc ttctcgttca acggcacgac cgtgaaggca ttcgcgggaa cggcgacgtc
     5461 ggcgagcgac gctttcgtct gctcgccctc gggggtgaac agcgacagac gcacgaccgc
     5521 ctcgtcgggg aaggggttgt agaggaggat tcgctcgtcc gcttcgagcg acgcggagcc
     5581 ggccgcgaag taccagttgg cgccggccgt ccgcgcgcag ccggcggcgc cggcacccgc
     5641 gccgggccgc tcggtcgacg tcaagaccgc gacccccgcc ggcgcgccgt agccgacgat
     5701 gtccgccggc tccgaacccg agacgtcgta gatcaacgcc ccctcgcgcg gcacctccgc
     5761 cgtctcgccc tcctgcggct cgatgccgac cgccaaccga tccccctcgg gggcgacgat
     5821 gacgaggtcg acgtccgccg ccccgcccgc caccgcgccg ggacagaaga ccgaatgcgc
     5881 ctcgaagccg ccgcggccac ccgcctctgc ctccgccggc ggtacgtcgc cgctcgtgag
     5941 gtccaagacg aggccggcgg cgagcagcag acccacccct gcggcgaagt acgcctcccg
     6001 acggcgctcc gtcacgtgcg cctcctccgc gcccggcgcg gcgccgcggc gccgatcacg
     6061 accagccaca tgaacaccag agagccgtac cacacgtatc gcaggggcga cgacgcgaac
     6121 cacacctcga cggtggcatc acccgcggga agcgcgaacg cgttccccca ccccgagtcg
     6181 acgcgcccga gccgctcctc gcctgctcgc gcctcccact gaggatggcg tgcctgcgcg
     6241 aggaagacca cgcccggtcc tgcggcgcgc ccgcggaacg gtcccgcggc gccctcgctc
     6301 cgcagctcgg ccacgggctc gacctcctcc tcccccacct ccgccagcgc gcccggcccc
     6361 tcgtcgagag cgcgggcata ggcggggagc tcgtcgaaga ccgcggcccg gggcaactcg
     6421 agctggttac ggaggacgtg ataggtcggc tccgttcgga tcaccgcgag gtcccgctgc
     6481 gccagccagc cgcgcgcgtc tggcccccgc tcgacgatga cgaagtggac gttgaaggcg
     6541 ccgagcaacc ggccgcctcg gtcggtctcg ccgctctcga tcgaccagat cacgcggcgc
     6601 aagcgcgtct ctccgggccc cgtggccggt tggaacagat cggtgagaag actgctgcgc
     6661 gcaccggtga tgaagtgctc ccccggcggc cgcagcgcgc tcggttcgtc cggcagccag
     6721 gtgtcgccga cccacagcgc gcggaactca ccgatctgtt gagcctccgc gtcgagcagc
     6781 gaggagacct gctgcaccac cggcgcctcg atcccgcccg acgtgcgtcc cgggtcccat
     6841 ccccccgcca aggtcgtggg ccccaggccc gttacggcga ggaacagtga gatgccgagc
     6901 accccgatcg tgagcgcgtg ctgccagccc agagccttgc gccggaggtc gagccggaaa
     6961 gcaccgatcg caagccccgc gagcccggcg aacgcgagcg acgcgaggac gcccgcctcg
     7021 gtcggcgccg cgacgatcgg ccgcaggacg ccagtcgaga tcagttcgac cacgatgccg
     7081 ctcgtgacca ccacgctcca gagcgcgaga gccatgcggc ggcgcgagcc ctccccgacc
     7141 agcaccgcca ccagcccgag cagcgggagc gcgaggccga agagcggcgg cccatcgggt
     7201 gtctgaccca gtagcgtgct ggccatgccg tggccgcgga acgacgccgc gaacagcgga
     7261 gcggtgtcat cgccgaggag gcgattcatg acccctcccg gtgcgaacca ggtcgtgctc
     7321 caaggaagca gcagcaccca cgcgagcgcg aggccgattc cggccgagac gagcccctgg
     7381 agggctcggc caccgcggtc gaggacggcg cgcgctatcg ccacgaggat cgcgaccagc
     7441 gcgtacagca ggagcgaacc ggggacgaac gcggccgata ccgcggcccc gagggcgacc
     7501 cgcg
//
LOCUS       DATLGG010000255         3080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_8800, whole genome shotgun sequence.
ACCESSION   DATLGG010000255 DATLGG010000000
VERSION     DATLGG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8800"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1946)
                     /locus_tag="VM638_07300"
     CDS             complement(177..1946)
                     /locus_tag="VM638_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12264.1"
                     /translation="MKRMLATLGPLALVLVAGLPPAGAQSIPATPVATQENVRHVANV
                     PGATGGHSVVEGNRLYVGAYGLGFRIFDISNPRSPRQIGQYIPGPHPDSNNTVDLGVR
                     ADAVPDAAVFGGRHIATLNGTSRRPNAAAGGVTVRGTQQTEFLDVTNPANPVLLHRFN
                     GPVTDGNTFGEAHNGDILDARKLWVPSGGRDSNILRIFDLSPVLQSPPQAPKLLARAD
                     PFNLWRDSPYRQNTGKPIGVNPSGSHTHDVELYGGVTVLLPEWEWVDQDEDGIPEPTY
                     AQKDIMLMAVSTDYAVDGKGANNGSVYVVDISNPADPVVINRWQNPNDAGDHIRYLHE
                     VQFLHGDFSTMIVTDEDLHSGCEGGRLYTVRISPDLMDATKLAEWAIGSAQADTPTCL
                     GSHVFSSHDRHVFMGAYTAGLQVLDLTNPAQPKRAGRYIPEGANSWGALYHAGVIYVG
                     DFGGRGLDVFEFIKDPVAKAFVKAANPGTRTTGGVAERGCRAPINDPYGPTNGTDGII
                     VPIPEWARDGKHTLKALGSSSAPYDLDIWFHTGTNCTSMGYPGGVDGTDASEPIPEEA
                     TMASIDLYTGAAQWVYATIVKTG"
     gene            complement(2099..>3080)
                     /locus_tag="VM638_07305"
     CDS             complement(2099..>3080)
                     /locus_tag="VM638_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12265.1"
                     /translation="PEWEWEDQDGDGQADPTYGKRDIALLAAAGGGNTSGAVYVIDIT
                     DPRAPVVISKWQNPTGAGHTPISYLHEAQFLHGDPNTIFVADEILGNNCSAGRLYVVH
                     ISDDLVHTAKRSEWTVGAGMHDLPVCMGSHVFSSQDRHVFMGAYHAGLQVIDLRDPAQ
                     PKRAGRYIAEGQNAWGALYHRGVVYVGDFGGRGLDVYEFIKDPVARGFLKVGNPTSRL
                     ATPGGGVTELTGACKADAPTNDLDGMLVQIPTSKRDGTATIKAFGTGAPGAYDVDIWF
                     YNANCAYLGGIREEGQEIGGDASGLILEGAAFAYVDLWAGTPQWVYAQLP"
BASE COUNT          431 a         1027 c         1099 g          523 t
ORIGIN      
        1 gcccgaaccg agccatgcca ctcaccgaga ccgtccgtcc tgacggagag gccgtcagct
       61 cggtcacctc gctcgacgag ccggcgaggc cggccgtcag cagcgagatc gtgagggcga
      121 cggtcgtcgg gaccgaagtg acggtgccga tcgccatcgc cctcccgtct ccctcctcag
      181 cccgtcttca cgatcgtggc gtagacccac tgcgcggcgc ccgtgtagag gtcgatcgat
      241 gccatcgtcg cctcctccgg gatcggctcg gacgcatcgg tgccgtcgac gccaccgggg
      301 tagcccatgc tcgtgcagtt ggtcccggtg tggaaccaga tgtcgaggtc gtagggcgcg
      361 gacgagctgc ccagcgcctt gagggtgtgc ttgccgtcac gcgcccactc ggggatcggg
      421 acgatgatgc cgtcggtgcc gttggtcggg ccgtacgggt cgttgatcgg cgcgcggcag
      481 ccgcgctcgg ctacgccccc cgtcgtgcgg gtccccgggt tcgcggcctt cacgaacgcc
      541 ttcgccacgg gatccttgat gaactcgaac acgtcgagcc cccggccgcc gaagtcgccc
      601 acgtagatca cgccggcgtg gtagagcgct ccccaggagt tcgccccttc ggggatgtag
      661 cgaccggcgc gcttcggctg cgccgggttc gtcaggtcga ggacctgcag ccccgcggtg
      721 tacgcgccca tgaagacgtg gcggtcgtgg gagctgaaca cgtgtgaccc gaggcaggtc
      781 ggcgtgtccg cctgcgccga gccgatcgcc cactcggcga gcttggtggc gtccatcagg
      841 tcgggcgaga tgcgaacggt gtagaggcgc ccgccctcgc agccgctgtg caggtcctcg
      901 tcggtgacga tcatcgtgct gaagtccccg tgcaggaact gcacctcgtg gaggtagcgg
      961 atgtggtcgc ccgcgtcgtt cgggttctgc caacggttga tcaccaccgg gtcggcgggg
     1021 ttgctgatgt cgacgacgta gaccgacccg ttgttcgcgc ccttcccgtc gaccgcgtag
     1081 tcggtcgaga ccgccatcag catgatgtcc ttctgggcgt aggtcggctc cgggatgccg
     1141 tcctcgtcct ggtccaccca ctcccactcc ggcaggagca cggtcacgcc tccgtagagc
     1201 tcgacgtcat gcgtgtgcga cccgctcggg ttcacgccga tcggcttgcc cgtgttctgg
     1261 cggtacggcg agtctcgcca gaggttgaag gggtcggcgc gggcaaggag cttcggcgct
     1321 tgcggcgggg actgcaggac cgggctgagg tcgaagatcc gcaggatgtt cgagtcccgg
     1381 ccaccggagg gaacccacag cttccgggca tcgaggatgt cgccgttgtg agcctcgccg
     1441 aacgtgttcc cgtcggtgac ggggccgttg aaccggtgaa gcaggaccgg gttcgcgggg
     1501 ttcgtcacgt cgaggaactc cgtctgctgc gtgccgcgca ccgtgacgcc gccggccgcc
     1561 gcgttgggac gacgtgaggt cccgttcagc gttgcgatgt gccggccgcc gaaaaccgcc
     1621 gcgtctggca cggcatcggc gcgaaccccg aggtcgaccg tgttgttgga atccgggtgc
     1681 ggtccgggga tgtactgacc gatctggcgc ggcgaccggg ggttcgagat gtcgaagatc
     1741 cggaacccga gcccgtatgc cccgacgtag agccggttgc cctcgacgac gctgtggccg
     1801 ccggtggccc caggcacgtt cgccacgtgg cgcacgttct cctgggtggc gaccggcgtg
     1861 gccgggatcg actgtgctcc cgctggcggg aggccggcga cgagcacgag ggcgagggga
     1921 ccaagcgtgg cgagcatccg cttcatcagt acctccagtt cgacagcgtc tgacggatag
     1981 gttcagcggc ggaccgccgt tctcctttgt gcaggtgcac aaaaggggcg ctccgggcgg
     2041 gaacgaccga ggggcctcct cgcggaggcc cctcgagacg cgtcgaacgg gacaggcgtt
     2101 aggggagctg ggcgtagacc cactggggcg tccccgccca cagatccacg taggcgaacg
     2161 cggcaccctc gaggatgaga cccgaggcgt ccccgccgat ctcttgcccc tcctcgcgga
     2221 taccgccgag gtaggcgcag ttcgcgttgt agaaccagat gtcgacgtcg taggcaccgg
     2281 gggcgccggt cccgaacgcc ttgatcgtgg cggtcccgtc gcgcttcgac gtcgggatct
     2341 gcacgagcat cccatcgagg tcgttcgtcg gggcgtcggc cttgcacgct cccgtcagct
     2401 cggtcacgcc cccgcccggg gtggccaggc gtgaggtcgg gttgcccacc ttgaggaagc
     2461 ccctcgcgac cgggtccttg atgaactcgt agacgtcgag cccgcgcccg ccgaagtcac
     2521 cgacgtagac gacgcccctg tggtagagcg ccccccaggc gttctgccct tcggcgatgt
     2581 agcggcctgc gcgcttcggc tgcgccgggt cacggagatc gatcacctgg agccccgcgt
     2641 ggtaggcacc catgaacacg tgtcgatcct gggagctgaa cacgtgcgag cccatgcaga
     2701 cgggcaggtc gtgcatcccg gctccgaccg tccactcgga tcgcttcgcg gtgtgcacga
     2761 ggtcgtcgga gatgtgaacg acgtagagcc gtccggccga gcagttgttg cccaggatct
     2821 cgtcggcgac gaagatcgtg ttggggtcgc cgtgcaggaa ctgggcctcg tgcaggtagc
     2881 tgatcggcgt gtggccggcg ccggtggggt tctgccactt gctgatgacg accggcgcac
     2941 gcgggtcggt gatgtcgatc acgtagacgg cgccgctcgt gttcccgccg ccggcggccg
     3001 cgaggagggc gatgtcgcgc ttgccgtagg tcgggtcagc ctggccgtcc ccgtcctggt
     3061 cttcccactc ccactccggt
//
LOCUS       DATLGG010000256         4309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_172132, whole genome shotgun sequence.
ACCESSION   DATLGG010000256 DATLGG010000000
VERSION     DATLGG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172132"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VM638_07310"
     CDS             complement(<1..308)
                     /locus_tag="VM638_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVM12266.1"
                     /translation="MTQAETPAPETEGRAQDLRRSEELFARMPDEHAREELVLLHRPL
                     ADYLARRFAGRGEAQEDLEQVAVLALIKAIDRFDPGREVKFSTYATATIVGELKRHFR
                     "
     gene            complement(295..780)
                     /locus_tag="VM638_07315"
     CDS             complement(295..780)
                     /locus_tag="VM638_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVM12267.1"
                     /translation="MRLTSCDRRLRAASARAGRIVYVRVKELFGLPVTITIPAQPEFV
                     HILRSVAAGVAARLDYSFDEIDDLRIALDEACAELLRSVPEVEALTVRLIPNEHGLEL
                     IARAEGSADGEWPPPRLEGSLAWQVLSALVDGEPAFVREQGKPTVRMSKRIGWSNGDP
                     G"
     gene            779..1111
                     /locus_tag="VM638_07320"
     CDS             779..1111
                     /locus_tag="VM638_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVM12268.1"
                     /translation="MDIQLDVERQPGAPVVRVSGEVDLFTAPKLKEGILDLLGDDTRR
                     LIVDLERVEFMDSTGLGVLVGALRRLKERDGTLALVCPQGPVLRVLDVTGLSKVFEIY
                     GSVADATA"
     gene            1108..1527
                     /locus_tag="VM638_07325"
     CDS             1108..1527
                     /locus_tag="VM638_07325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12269.1"
                     /translation="MSTTPLELEIPPIPAYVGTVRLFVAAVARQAGIEEDEVDDLKVM
                     VSEACTGAIRNRSEAASEEGVRVQMTPGDGTLLVEVPRAFDEDAVSSEGTTDNIARGL
                     GAELVRALFPQAELVPDGAGMTSLRVVLATGGSAGSG"
     gene            1569..1997
                     /locus_tag="VM638_07330"
     CDS             1569..1997
                     /locus_tag="VM638_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12270.1"
                     /translation="MDTMYRSDAVKIDLRLPRTVDAARVARRSLDGVRHRLDRARFDD
                     LRLLVSELVTNSVLHAGLDPDSWVEVHCEIGVDVVRVEVTDPGSASVPQPRFPEPDEV
                     SGRGLFMVQSLSDRWGVSNEGGTRVWFELRLRGDRRVSLV"
     gene            2099..2254
                     /locus_tag="VM638_07335"
     CDS             2099..2254
                     /locus_tag="VM638_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12271.1"
                     /translation="MPRCPNCHAARIVIVVGTDRRSFCVECGTKWIQDGSEQRSVETT
                     STAPQPA"
     gene            2264..2437
                     /locus_tag="VM638_07340"
     CDS             2264..2437
                     /locus_tag="VM638_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HVM12272.1"
                     /translation="MHELLGFVAQARIELSLPAILALVAGILILIWPRVLNYIVAIYL
                     IAVGLIELFDLRI"
     gene            2492..2950
                     /locus_tag="VM638_07345"
     CDS             2492..2950
                     /locus_tag="VM638_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM12273.1"
                     /translation="MSQDVRVLVIEDEDSIRRLIRAALGTEGYQVTETAHGEEGVALL
                     EQESFDLVILDIQLPGISGWDVLETMRARGLRDQVRVVMLTAQSQEREILRGWRMGVD
                     HYCLKPFEPEEFLATVDHVLSASSEQLERDRQQQLQRTQLLHLVDSVFDT"
     gene            complement(2947..>4309)
                     /locus_tag="VM638_07350"
     CDS             complement(2947..>4309)
                     /locus_tag="VM638_07350"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HVM12274.1"
                     /translation="DGGDADRSLDRVSSVEGLASSSRYRTSTTTVARGVTLKRIRDRR
                     GPRRIFVLTVDPRNRPTIDTGLARNRLPEFERTSSIARRHGAIAAVNGDFGLPSGRPA
                     HAFAEDADLKQTSHAYGYNFAVTRDGSAAFIGHREVTLASVADARGDVWDVDRWNDGA
                     PTSGQIAAFSRAARRGDGTWVERPPAHACSARLSPGSPPRWTTGETGVQTTHVVTAVG
                     CESDRLMPGSGIVLSARPHSPEAAFVRSLAVDGTITLSWSLGWRHTLDTIGGYPELVR
                     DGRRVAGDCSQPLCGRHPRTGVGIRRDGRIMLVVVDGRRRGWSVGMTLREFGAEMERL
                     GAVAALNLDGGGSSTMWIRGRIVNRPSDGSERAISSAILVLPGGDAGETTPGASELGE
                     DPAEEEMGATGDSERAGDLALSDPASTGGFLDALDAGVGGASGPLAPDLAELLERVAA
                     AFG"
BASE COUNT          623 a         1539 c         1521 g          626 t
ORIGIN      
        1 tcccggaagt gccgcttgag ctcccccacg atcgtcgcgg tcgcgtaggt cgagaacttc
       61 acctcgcggc cggggtcgaa gcgatcgatc gccttgatca gcgcgaggac cgcgacctgc
      121 tcgaggtcct cctgggcctc gccgcgcccg gcgaagcgac gcgccaggta gtcggcgagc
      181 ggccggtgaa gcaggacgag ctcctcccga gcgtgctcgt ccggcatccg ggcgaacagc
      241 tcctccgagc ggcggaggtc ctgggcgcga ccctcggtct ccggcgccgg cgtctcagcc
      301 tgggtcaccg ttgctccacc cgatccgctt cgacatccgg acggtcggct tgccctgctc
      361 gcggacgaag gcgggctcgc cgtccacgag ggcggacaga acctgccagg cgagggatcc
      421 ctcgagccga ggcggcggcc actcgccgtc cgccgacccc tcagcgcgcg cgatcagctc
      481 cagcccgtgc tcgttcggga tcaggcggac cgtgagcgcc tcgacctcgg ggaccgagcg
      541 caggagctcg gcgcatgcct cgtcgagcgc gatcctgagg tcgtcgatct catcgaacga
      601 gtagtcgagg cgggcggcca cgccggcggc caccgagcgc aggatgtgga cgaactcggg
      661 ctgcgccggg atcgtgatgg tgacgggtag gccgaagagc tccttcacgc ggacgtatac
      721 tatcctgccg gcacgggcgg acgccgcccg gagccgccgg tcgcaggagg tgaggcgcat
      781 cgacatccag ctggacgttg aacggcagcc cggggcaccg gtcgtccgcg tgagcggcga
      841 ggtcgacctg ttcaccgcgc cgaagctcaa ggaggggatc ctcgacctgc tcggcgacga
      901 cacccgtcgc ctgatcgtgg acctcgagcg ggtcgagttc atggattcga ccggccttgg
      961 ggtgctggtg ggggccctcc ggaggctgaa ggagcgggac gggacactcg ccctcgtgtg
     1021 tccgcagggt ccggtccttc gggtcctcga cgtcaccggg ctgagcaagg tgttcgagat
     1081 ctacggctcg gtcgccgacg caaccgcgtg agcacgaccc ccctcgagct cgagatcccg
     1141 cccatcccgg cctacgtcgg gacggtgagg ctcttcgtgg ccgcggtggc gcgccaggcc
     1201 ggcatcgaag aggacgaggt cgacgacctc aaggtcatgg tctccgaggc gtgcaccggg
     1261 gcgatccgga accgctcgga ggcggcgagc gaggagggtg tccgggtcca gatgacgccc
     1321 ggcgacggga cgctcctcgt cgaggtgccg cgcgccttcg acgaggacgc cgtctcgtcc
     1381 gagggcacca cggacaacat cgcgcgcggg ctcggggccg agctcgtcag ggcgctgttc
     1441 ccgcaggccg agctggtccc tgacggggcc gggatgacct ccctgcgggt cgtgctcgcc
     1501 acgggggggt cggccggatc ggggtgaatg ccctggcgca cggtgggtaa agaggactcg
     1561 gtgctgccat ggacacgatg tacagatcgg atgccgtgaa gatcgacctc cggctcccgc
     1621 ggacggttga cgccgcccgc gtggcccgtc ggagcctcga cggcgtccgc catcgcctgg
     1681 accgagcgag gttcgacgac ctgcgcctcc tcgtcagcga gctcgtgacg aactccgtcc
     1741 tgcacgccgg cctcgacccc gactcgtggg tggaggtcca ctgcgagatc ggcgtcgacg
     1801 tcgtccgggt cgaggtgacc gaccccggat ccgcgagcgt gccgcagccc aggttccccg
     1861 agccggacga ggtctccggc cgcggcctct tcatggtcca gagcctctcc gaccgctggg
     1921 gcgtctccaa cgagggaggc acccgggtct ggttcgagct caggctccgc ggcgaccggc
     1981 gcgtctcgct cgtctgagtc tgccgctccc tcgcgactgg tttgacgggt tcggcgggcg
     2041 ggtagacctg gtccaccgcc ggatcggtgc cggcccctga atgaaggagg atccacagat
     2101 gccgcgttgt ccgaactgcc atgcggcccg catcgtgatc gtcgtcggaa ccgaccggcg
     2161 ctcgttctgc gtcgagtgcg ggacgaagtg gatccaggac gggagcgagc agcgttcggt
     2221 cgagacgacc tcgacagcgc cgcagccggc ctagggacgg gggatgcacg agctcctcgg
     2281 cttcgtcgca caagcgcgga tcgagctctc cctccccgcg atcctcgcgc tcgtcgcggg
     2341 catcctcatc ctcatctggc cgcgcgtcct caactacatc gtcgcgatct acctcatcgc
     2401 ggtcgggttg atcgagctct tcgacctccg catctgaggc tcaaggcgcg gccctcccgc
     2461 gtcgattcct tccggtgaga gaggaggagc cgtgtcccag gacgtccgcg tgctcgtcat
     2521 cgaagatgag gattcgatcc gccgcttgat ccgcgccgcg cttggtaccg agggctacca
     2581 ggtcaccgag acggcgcacg gcgaggaggg ggtcgccctg ctcgagcagg agagcttcga
     2641 cctcgtcatc ctcgacatcc agcttcccgg gatcagcggg tgggacgtgc tcgagacgat
     2701 gcgcgcgcgg gggctccgcg accaggtccg ggtggtgatg ctcaccgcgc agagccagga
     2761 gcgggagatc ctccgcgggt ggcggatggg cgtcgaccac tactgcctga agcccttcga
     2821 gcccgaggag ttcctggcga ccgtcgacca cgtcctctcg gcctcgagcg aacagctcga
     2881 acgcgatcgc cagcagcagc tccagcgcac gcagctgctg cacctcgtcg acagcgtctt
     2941 cgacacctag ccgaaggccg cggccacacg ctcgagcagc tccgcgaggt cgggagcgag
     3001 cgggcccgac gctccgccga cccccgcgtc gagcgcatcc aggaaccctc ccgtggatgc
     3061 cggatcgctc agcgcgagat caccggcgcg ttcgctgtcc ccggtcgcac ccatctcctc
     3121 ctccgccgga tcctcgccga gctcggaggc tccgggcgtc gtctcaccgg cgtcgccgcc
     3181 cggcaggacg aggatcgccg aggagatcgc gcgctcggac ccgtccgacg ggcggttgac
     3241 gatcctgccg cggatccaca tcgtgctgga gcccccgccg tcgaggttga gcgccgcgac
     3301 cgcaccgaga cgctccatct cggccccgaa ctcgcgcagg gtcatcccga ccgaccaccc
     3361 gcggcgacgg ccgtcgacca cgacgagcat gatccgcccg tcgcgccgga tgcccacgcc
     3421 cgtgcgcggg tggcgcccgc agaggggctg ggagcaatcc ccggcgacgc ggcgcccgtc
     3481 gcggaccagt tccggatagc caccgatcgt gtcgagcgtg tggcgccatc cgagggacca
     3541 cgagagcgtg atggtgccat cgaccgccag cgagcggacg aacgcggcct caggcgagtg
     3601 cggccgcgcc gacaggacga tccccgaccc cggcatcagc cggtcgctct cgcacccgac
     3661 cgccgtcacg acgtgcgtcg tctggacccc cgtctccccc gtggtccacc gagggggcga
     3721 gccgggcgac aggcgcgcgg agcaggcgtg tgccggcggt cgctcgaccc aggtcccgtc
     3781 gccacgacgc gcggcccggg agaaggcggc gatctggccg ctcgtcggtg cgccatcgtt
     3841 ccaccgatcc acatcccaga cgtcccctcg ggcgtccgcc accgaagcga gggtcacctc
     3901 ccgatggccg atgaacgcgg ccgagccgtc ccgcgtcacc gcgaagttgt agccgtacgc
     3961 gtgcgaggtc tgcttgaggt cggcgtcctc ggcgaaggcg tgggccggcc ggcccgatgg
     4021 aaggccgaag tcaccgttca cggccgcgat ggcgccgtgc cggcgtgcga tcgagctcgt
     4081 ccgttcgaac tccgggagac ggttcctggc gagcccggtg tcgatcgtcg gacggttccg
     4141 tggatccacc gtcagcacga agatccgtcg aggaccccga cggtcgcgga tccgcttcag
     4201 ggtcaccccc ctcgcgaccg tcgtggtgct cgtgcggtag cgggagctcg acgcgagacc
     4261 ttcgacgctc gagacgcggt cgaggctccg atcagcgtcg cccccatcg
//
LOCUS       DATLGG010000257         5034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_75276, whole genome shotgun sequence.
ACCESSION   DATLGG010000257 DATLGG010000000
VERSION     DATLGG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75276"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VM638_07355"
     CDS             <1..877
                     /locus_tag="VM638_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HVM12275.1"
                     /translation="GGPGGIRFEDLGFEGGFGDLFGDLFGTAARGGARTGRGRRGADL
                     QTEVRIAFEQALEGATVPVRVSGAAPCATCGGSGAKPGTEVRTCARCAGAGTVAVDQG
                     VFSLTRACPSCGGAGRVVEHPCPTCGGSGATRATRELKVKIPAGVEDGARIRLSGRGE
                     AGPPGGRPGDLYVLVEVAPHPFFGRRGADLLLTLPVTYPEAALGSKVQVPTPSGEVTL
                     KIPAGTASGKTFRIRGRGAPKRGGGRGDLLVTTEVDVPPRLSREQKDLLRRLAEANGH
                     DPRAHLGVERRGATT"
     gene            874..1281
                     /locus_tag="VM638_07360"
     CDS             874..1281
                     /locus_tag="VM638_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007318059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVM12276.1"
                     /translation="MSDDIRTDLPDDELDELDELDERALYVISVAAELAGVHPQTLRI
                     YERKGLLSPQRSAGNSRRYSERDIAQLRRIQRMTQSGINLEGVKRIIELQTEIAEMRR
                     EMQELQTEIDARGPRGDLIPIRDLPRLPHTEEG"
     gene            1283..3856
                     /gene="clpB"
                     /locus_tag="VM638_07365"
     CDS             1283..3856
                     /gene="clpB"
                     /locus_tag="VM638_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HVM12277.1"
                     /translation="MDPQRLTLRSRNALEGAQAQARARNHPQVEPAHLLHALLSDPEG
                     VVMPLLHAVGHSPRSLRDRVEEVLDRMPRAYGAGGEVVGSPSLGELLRRAGDEAAALS
                     DEYVSTEHLLLAMAGSGDAVAALLADAGLDRDGILRALAEVRGRQRVTDENPEEKYQA
                     LERYSRDLTDLARRGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAQRVVDGDVPEGLKGKRVVALDIGALLAGAKYRGEFEERFKAVLREIAESEGEIVTF
                     IDEMHTVVGAGAAEGAVDAGNMLKPMLARGELRAIGATTLDEYRKHVEKDPALERRFQ
                     PILVAPPSVEDAIAILRGLKERYEVHHGVRIKDEALVAAAILSDRYVSGRFLPDKAID
                     LIDEAASRLRIEIDSMPTEIDEVDRRIRQLEIERAALQRETDEPSRERLGKLEQELAD
                     LNERTMAMRAHWDREKEHIDRIRALKSEIEDARAEAERAERDADLQRAAELRYGRLVE
                     LEKRLEEENRLLEEVQAERRMLKEEVDDDDIAEVVSAWTGIPVSRLLEGEVAKLVRME
                     EALHERVVGQDEAVTAVANAIRRARAGLSDPNRPIGSFIFLGPTGVGKTELARALAEF
                     LFDDERAMVRIDMSEYQERHAVARLIGAPPGYVGYEEGGQLSEAVRRRPYTVVLLDEI
                     EKAHPDAFNVLLQVLDDGRLTDGQGRTVDFRNTVLIMTSNLGSRWFFDETIAPQERRE
                     RVRQELHASFRPEFLNRVDETIVFEPLDKAELAAIVDIQLRALERRLADRRLDIEITE
                     GARAFLADRGYDPAFGARPLKRLIQREIQDPLAMKLLAGEIEEGRTIVVDRHDDGLSI
                     R"
     gene            3896..4534
                     /locus_tag="VM638_07370"
     CDS             3896..4534
                     /locus_tag="VM638_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVM12278.1"
                     /translation="MIALWVVLGLVALLLLWGVVSYNRFVAQRNAIRDAFANIDTELR
                     RRYDLIPNLVETVRGYAGHERATLEAVTNARAAAVAAGGSPAEQAAAEGPLVAALRQL
                     MAVAENYPQLRANESFVALQGELATTEDRIQNVRRVYNDQVRAYNERVQSVPSNIIAS
                     MFGFREEQFFEIAEALRPEVERAPQVDFGTQPPGGAASQPGQAQPGETPPPA"
     gene            complement(4536..>5034)
                     /locus_tag="VM638_07375"
     CDS             complement(4536..>5034)
                     /locus_tag="VM638_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12279.1"
                     /translation="FSLRVFDAGDGGTGVFYAFRGLGALIGPFLARPFVRDLPTMFRA
                     IGASIGIYGVFYAVFPFAPTLWTAAPLVLLAHLGGGAQWMMTTYGLQRIVPDRLRGRV
                     FSFDFGLVTLTIAMSNLAAGWAAGFLDLAVVMWSLAGISLAYAGAWTAATTGIRRRER
                     ERSGA"
BASE COUNT          779 a         1775 c         1847 g          633 t
ORIGIN      
        1 gggtggcccc ggcgggatcc ggttcgagga cctcggcttc gagggcggat tcggtgacct
       61 gttcggcgac ctgttcggga cggcggcccg cggtggcgcc cggacgggac gcggccgccg
      121 gggcgccgac ctccagacgg aggtgcggat cgccttcgag caggccctcg agggggcgac
      181 cgtccccgtc cgcgtgagcg gcgcggctcc ctgcgccacc tgcggcggat ccggcgcgaa
      241 gcccggcacc gaggtccgca cctgcgcgcg ctgcgccggc gccgggacgg tcgcggtcga
      301 ccagggggtc ttctccctca cgcgcgcctg cccatcctgt ggcggggccg gccgggtcgt
      361 cgagcatccg tgcccgacct gtggcgggtc gggggcgacg cgggcgaccc gggagctgaa
      421 ggtgaagatc ccggccggcg tggaggacgg ggcacgcatc cgtctctccg ggcgcggcga
      481 ggcgggcccg ccgggtggcc gtccgggcga cctctacgtg ctcgtcgagg tggcgccgca
      541 tcccttcttc ggacgccgcg gagcggacct cctgctgacg cttccggtga cgtaccccga
      601 ggcggcgctc ggctcgaagg tccaggtgcc gacgccgagc ggcgaggtga cgctcaagat
      661 ccccgccggg accgcgagcg gcaagacctt ccggatccgc ggtcgcggcg ccccgaagcg
      721 cggcggcggg cgtggcgacc tgctcgtgac gaccgaggtc gacgtcccac ctcgcctgtc
      781 gcgcgagcag aaggacctcc tccgccggct cgcggaggcg aacgggcatg acccgagggc
      841 gcacctcggt gtcgagcgac gaggagccac gacatgagcg acgacatccg cacggacctc
      901 cccgacgacg agctcgacga gctcgatgag ctcgacgagc gagcgctcta cgtgatcagc
      961 gtcgcggcgg agctcgccgg cgttcacccg cagaccctcc ggatctacga gcggaagggg
     1021 ctcctcagcc cgcagcgcag cgccggcaac agccgccgct actcggagcg cgacatcgcc
     1081 cagcttcgcc ggatccagcg catgacccag tccgggatca acctcgaggg tgtgaagcgg
     1141 atcatcgagc tccagacgga gatcgccgag atgcgccgcg agatgcagga gctccagacc
     1201 gagatcgatg cgcgggggcc gcggggcgac ctcatcccga tccgggatct cccccggctg
     1261 ccgcacacgg aggagggctg acgtggaccc gcagcgactg acgctgagat cacggaacgc
     1321 gctcgagggg gcgcaggcgc aggcgcgggc gcggaaccac ccgcaggtgg aaccggctca
     1381 tctcctgcac gcgctcctgt ccgatcccga gggggtcgtg atgccgctgc tccacgcggt
     1441 ggggcattcc ccgcgatcgc tccgcgaccg ggtcgaggag gtcctcgacc ggatgccacg
     1501 cgcctacggc gcgggcggcg aggtcgtcgg ctccccgtcg ctcggcgagc tcctccgccg
     1561 cgccggcgac gaggccgccg cgttgagcga cgagtacgtc tccaccgagc acctgctcct
     1621 ggccatggcg ggctcagggg atgcggtcgc ggccctcctc gccgacgccg gcctcgaccg
     1681 cgacggcatc ctgcgcgcgc tcgccgaggt tcgcggacgc cagcgcgtca ccgacgagaa
     1741 ccccgaggag aagtaccagg cgctggagcg gtactcccgc gacctcacgg atctcgcgcg
     1801 ccgcggcaag ctcgacccgg tgatcggccg cgacgacgag atccgccggg tgatccaggt
     1861 gctgtcgcgc cggacgaaga acaaccccgt gctgatcggc gagccggggg tcggcaagac
     1921 ggcgatcgcg gaggggctcg cccagcgggt cgtggacggg gacgttccgg agggcctgaa
     1981 gggcaagcgc gtggtggcgc tcgacatcgg agcgctcctc gccggcgcga agtaccgcgg
     2041 cgagttcgag gagcggttca aggcggtgct gcgtgagatc gccgagagcg agggcgagat
     2101 cgtcacgttc atcgacgaga tgcacacggt cgtcggtgcc ggcgcggccg agggcgccgt
     2161 cgacgcgggg aacatgctca agccgatgct ggcccgcggc gagctgcggg cgatcggcgc
     2221 gacgaccctc gacgagtacc gcaagcacgt cgagaaggac cccgcgctcg agcgccgctt
     2281 ccagccgatc ctcgtcgcgc cgccgtcggt cgaggacgcg atcgcgatcc tccgagggct
     2341 gaaggagcgc tacgaggtcc atcacggggt ccggatcaag gacgaggcgc tcgtcgccgc
     2401 cgcgatcctc tcggaccgct acgtgagcgg acgcttcctg cccgacaagg cgatcgacct
     2461 gatcgacgag gcggcgagcc gcctgcggat cgagatcgac tcgatgccga ccgagatcga
     2521 cgaggtcgac cggcggatcc gccagctcga gatcgagcgc gccgcgctgc agcgcgagac
     2581 cgacgagccc tcccgcgagc gcctcggcaa gctcgagcag gagctcgcgg acctgaacga
     2641 gcggacgatg gcgatgcggg cgcactggga ccgcgagaag gagcacatcg accggatccg
     2701 cgccctgaag tccgagatcg aggacgcgcg ggccgaggcc gagcgcgccg agcgggatgc
     2761 cgatctccag cgggccgccg agctgcgcta cggccgcctc gtcgagctcg agaagcggct
     2821 cgaggaggag aaccgactcc tcgaggaggt ccaggcggag cggcggatgc tgaaggagga
     2881 ggtcgacgac gacgacatcg cggaggtcgt ctcggcctgg acggggatcc cggtgtctcg
     2941 gctgctcgag ggtgaggtcg cgaagctcgt gcggatggag gaggcgctcc acgagcgcgt
     3001 ggtcggccag gacgaggccg tgaccgccgt cgcgaacgcg atccgccggg cccgcgccgg
     3061 actctcggat ccgaaccgcc cgatcggctc gttcatcttc ctcggaccga ccggcgtcgg
     3121 gaagacggag ctggctcgcg cgctggcgga gttcctcttc gacgacgagc gcgcgatggt
     3181 gcgcatcgac atgagcgagt accaggagcg gcacgccgtc gcgcggctca tcggggcgcc
     3241 gcccggctac gtcggctacg aggaaggggg ccagctgagc gaggcggtcc ggcggcgccc
     3301 ctacaccgtc gtgctgctcg acgagatcga gaaggcgcat ccagacgcct tcaacgtcct
     3361 gctccaggtc ctcgacgacg ggcgcctgac ggacggacag gggcgcacgg tcgacttccg
     3421 caacaccgtg ctgatcatga cctcgaacct cgggagccgg tggttcttcg acgagaccat
     3481 cgcgccgcag gagcggcgcg agcgcgtccg ccaggagctg cacgcgtcgt tccggccgga
     3541 gttcctgaac cgcgtcgacg agaccatcgt gttcgagccg ctcgacaagg cggagctggc
     3601 ggcgatcgtc gacatccagc tccgggccct cgaacggcgc ctggccgatc gacgcctcga
     3661 catcgagatc accgagggcg cgcgcgcctt cctcgcggat cgtggctacg acccggcctt
     3721 cggcgcacgg ccgctcaagc ggttgatcca gcgagagatc caggacccgc tcgcgatgaa
     3781 gctcctcgcc ggggagatcg aggagggtcg gacgatcgtc gtcgaccgcc acgacgacgg
     3841 gctctcgatc cgctgatgct cgcttcgccg gttccgctgg cgtaggattc gccccgtgat
     3901 cgcgctgtgg gtcgtcctgg ggctggtcgc gctcctcctc ctgtggggtg tcgtctccta
     3961 caatcgcttc gtcgcccagc ggaacgctat ccgtgacgcg ttcgcgaaca tcgacaccga
     4021 gcttcgccgt cgctacgacc tgatcccgaa cctggtcgag acggtgcgcg ggtacgcggg
     4081 acacgaacgg gcgacgctcg aggccgtgac gaacgcgcgc gccgccgcgg tcgccgcggg
     4141 aggctccccg gccgagcagg cggcggccga gggcccgctc gtcgccgcgc tccgacagct
     4201 catggccgtc gccgagaact acccccagct ccgggcgaac gagagcttcg tcgcccttca
     4261 gggcgagctg gcgaccaccg aggaccggat ccagaacgtg cggcgcgtct acaacgacca
     4321 ggtccgcgcg tacaacgagc gcgtccagtc cgtgccgtcc aacatcatcg cttcgatgtt
     4381 tggcttccgg gaggagcagt tcttcgagat cgccgaggcg ctccgtcccg aggtggagcg
     4441 ggctccccag gtcgatttcg gcacccagcc tccgggcggt gcggcatccc agccgggtca
     4501 ggcccagccc ggcgagacgc ctccgcccgc ctagctcagg cccccgaccg ctcccgctcc
     4561 cgcctccgga tgcccgtcgt cgccgccgtc catgcgccgg cgtaggcgag cgagatgccc
     4621 gccaagctcc acatcacgac cgcgaggtcg aggaagccgg cggcccagcc tgcggcgagg
     4681 ttcgacatcg cgatcgtgag cgtcacgagc ccgaagtcga aggagaagac gcggcctcgc
     4741 aggcggtcgg ggacgatccg ctgcagcccg taggtcgtca tcatccactg cgcgccgccg
     4801 ccgaggtgcg cgagcagcac gagtggcgcc gccgtccaca acgtgggagc gaacgggaac
     4861 acggcgtaga acacgccgta gatcccgatc gaggcgccga tcgcccggaa catcgtcggg
     4921 agatcccgca cgaacggccg ggcgaggaag gggccgatga gcgcgccgag tccgcggaac
     4981 gcgtagaaca cgcccgtccc gccgtcgccg gcgtcgaaca cgcggagcga gaag
//
LOCUS       DATLGG010000258        12221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_120732, whole genome shotgun sequence.
ACCESSION   DATLGG010000258 DATLGG010000000
VERSION     DATLGG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120732"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VM638_07380"
     CDS             complement(<1..1198)
                     /locus_tag="VM638_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12280.1"
                     /translation="MTPRLRRALAAAGGPLLIAGVALFLLRGFWLFGRVSLQHVDPLP
                     FWLPMHCHLGETLASGSIPAWNPHVLGGVPFAADPQSGWMYLPAMLLYAALPCTTAFG
                     WFVTLQPVLAGVGLYWFLRVEGLSRPAATVGGLALAGGIAASRVAVTLPFSGSVAWTA
                     VMLGCAARFFRAPGWSGRLGWSVAVAAAWGQVAAAHLSHGLVVATLALAAYAIAAGGR
                     VRPRTRVAWLRAALLLAALVLVNLAFLAPRVPYLARSTLSLGYGGLDALAADLGSPVG
                     SSAEVGASVSPPWILGFATAPGAYLGMAALLLVPAAWALRERRRLAIWIGAFALLCYL
                     LPLRAVASAVASWGAWLPFADFYVHEPSRFRYGVLLALPILAALGIEAWRRGAASARW
                     VAVGAAA"
     gene            complement(1195..2505)
                     /locus_tag="VM638_07385"
     CDS             complement(1195..2505)
                     /locus_tag="VM638_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVM12281.1"
                     /translation="MKQPDLSVVIPVWAREHNLPRLIPAVRSAVRGAVDDHEIVVCSA
                     DASLRARVEALGARFVLAGGRGYGEILRAGFAAAEGAHVLTMDADFSHRPGYVRTMWD
                     HRERGEVLIGSRYVRGAFPEMRFLRRSLSRALNLLYRKALALPYRDLSSGFRMFRAPV
                     LADIGPPRGKGLDILPEMIVKAYCQGWKIEEVPFWYRGARPLTRMRMLRLGFGYLGTL
                     GKAFGLRNSVKAADYDHRAFDSWIPLQRYWQRARFRIIQDYMTGEGRVLDIGCGSSRI
                     VQTLPRVVGMDLALRKLRWLRAPGRPLLQGDMNRLPFRDGAFDAVVNSEVIEHIPRED
                     VRLEELVRVIRPGGALILGTPDYGKRLWRTLEWIYGKIFPGGYVKEHINRYDHDELRG
                     LLEDMGLAIEDVRYVGRSEMIFKARVPAGDRVSEAAPEPQPLGR"
     gene            2573..3403
                     /locus_tag="VM638_07390"
     CDS             2573..3403
                     /locus_tag="VM638_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVM12282.1"
                     /translation="MADRAELIDRLEGIARELRITDLEMIAGAGSGHPGGTLSAADMI
                     AALYFHVLRVRPEEPEWPDRDRFVLSKGHCIPIVYAAMAKRGFFDAELLTTLRDLGSP
                     LQGHPDRRRLPGVEASTGSLGQGLSVAVGMALAAKVDGASWRVFCMLGDGEIQAGQVW
                     EAAMFAPKHGLDNLIAILDHNQVQQTGRVEDILDVAPVAEKWAAFGWHVIDIDGHDMG
                     QIVDALDEADGADRPAIVVSRTKKGKGVSWMELNSAWHGKATKPEETERALAELRGQG
                     "
     gene            3403..4332
                     /locus_tag="VM638_07395"
     CDS             3403..4332
                     /locus_tag="VM638_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVM12283.1"
                     /translation="MGAATRDAFGEAILEVGADERIVVLTGDLKDSTRTEDFAEKYPE
                     RFFDAGIAESNMVGMAAGLALSGKIAWACSFSCFVTGRFETIRVSVAYQEANVRIVGT
                     HSGIGVGEDGYSQMAQEDIAALRALPNMAIVNPADAAETRRAVRALVEHEGPAFLRLT
                     RQGVDDVTGEDGAFDFGRIATLRDGSDVAIVGTGATVAEALKAADALAAEGVSARVMN
                     AHTLKPFDADAILAAAADCGRIVTVEDHSVHGGLGSAVAEVLASEGSPARLRVIGTTT
                     FGESGSPAALYEKHGLSGARVAQATRGFLNAST"
     gene            4426..5112
                     /gene="ftsE"
                     /locus_tag="VM638_07400"
     CDS             4426..5112
                     /gene="ftsE"
                     /locus_tag="VM638_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HVM12284.1"
                     /translation="MIKTEHVTKAYKDSVYALDDVSVEIGKGEFVFIVGPSGSGKSTF
                     IRLMLKEEEPTAGNIWVAGKNIAKLSPWKVPYLRRNIGTVFQDFKLLPDRTVFDNVAF
                     ALEVIGKPRHIIDKRVPEILELVGLGDKLGNYPDELSGGEQQRVSVARAFVNRPLILL
                     ADEPTGNLDPSTSVDIMKLLDRINRTGTTVIMATHDNAIVDAMRKRVIELDSGKVVRD
                     QARGVYGYGA"
     gene            5105..5980
                     /gene="ftsX"
                     /locus_tag="VM638_07405"
     CDS             5105..5980
                     /gene="ftsX"
                     /locus_tag="VM638_07405"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HVM12285.1"
                     /translation="MRRFRYFFRETFTALRRNALVAFAAISTMFISLFLLGGSLLVQK
                     QVELLVGEWGSKVEVSVFLREDASGDEIQALTREIEGLPEVQQVFFESKQQAYQRFQR
                     LFRDSPALVENVDPESMPQSFRVKLVDPEQFSVIRARLAGQPAIDEIRDEQRVLKRLF
                     AVTAVLRTGVMGVAFIMLIAAAGLIGNTVRMAVFARRKEIAIMKLVGATNWFIRVPFL
                     IEGAVGGLLGAGLAVLGLFAMKMLFIDPLRGNISFTPWVGTGAVLETVPWLVGVGILM
                     AVIASLFAMRRFLEV"
     gene            6058..7254
                     /locus_tag="VM638_07410"
     CDS             6058..7254
                     /locus_tag="VM638_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVM12286.1"
                     /translation="MRRIALIMTLVLAAQVPGGAFVSARPNDPRLERIEAEIRHKRQQ
                     IKHAEARRQGLLEEISRSDARRDDLTREIEDLQASLAETRTRLADTRASLRQAKDLIE
                     RWSRRLAAAEESLLQRREDLGRRAANAYKLGAAGYMDVILGAEDLRSLTDRVQFVDTV
                     LLADATFLRGLEVARELVEERRSTVREQERDLRAQERRVEREEQRLEELKAEREALRN
                     EIDMEIAHRHDLVEGIEQNKEEYERAVRELEAESAWITAQLRGSGSTGSGQIGGKLHW
                     PTAGSIGSGFGWRTHPIFGTRRFHAGVDIGAPCGQAIYAGESGRVISSGWQGGYGQTI
                     VIDHGRGLSTLYAHQSSLGVGSGATVRRAQRIGSVGTTGWSTGCHLHFEVRVNGNPVD
                     PVPYLT"
     gene            7270..7737
                     /gene="smpB"
                     /locus_tag="VM638_07415"
     CDS             7270..7737
                     /gene="smpB"
                     /locus_tag="VM638_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HVM12287.1"
                     /translation="MKGQHTVATNRRARHDYAIDETLEAGLVLTGAEVKSLRAGRASL
                     ADAYGRVDGGEVWIEGLHIPPYEHDSRDRGRYEEKRPRKLLLHRAEIDRLVGKIKERG
                     MTLVPMRLYFVRGRAKLELGLGRGKRQYEKRRSIAEKEQRREIERALSARRRG"
     gene            7780..8130
                     /gene="ssrA"
                     /locus_tag="VM638_07420"
     tmRNA           7780..8130
                     /gene="ssrA"
                     /locus_tag="VM638_07420"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8152..9018)
                     /locus_tag="VM638_07425"
     CDS             complement(8152..9018)
                     /locus_tag="VM638_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVM12288.1"
                     /translation="MRARIWGSRGSLAAPGPETVRYGGNTSCVEVRLANDTLLILDAG
                     TGIRPLGVALGADVPRRIHLFLSHLHLDHLQGLPFFGPLWNPDVEIHIWGPPSPTRTL
                     EQSIARYLSPPLFPVHLQDVPCRLRFHDARGDLGIEEAVVRAMPVSHQGPTVGIRIES
                     EGRILAYIPDHEPGLAVDLRAVEPAWISGHGVARGADVLIHDSQYTEDEYLRKVGWGH
                     SSLDHVVQFARIADVGQLVMFHHDPLHTDDELERMLDRARELWGERPNPPVLAHEGMD
                     LDLSALPKRASA"
     gene            complement(9019..9417)
                     /locus_tag="VM638_07430"
     CDS             complement(9019..9417)
                     /locus_tag="VM638_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12289.1"
                     /translation="MADEHNLVLILARGLASSIATPVFLVDPEGTLVYFNEPAEGILG
                     QSYADSGSMTLEEWGTRFRPIDPGSGTPIPPEDLPLGIALTHRRPAHGPLHIVGADGV
                     AREIAVTALPLFAKSDEFIGAMAIFWEAEG"
     gene            9483..10541
                     /gene="selD"
                     /locus_tag="VM638_07435"
     CDS             9483..10541
                     /gene="selD"
                     /locus_tag="VM638_07435"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HVM12290.1"
                     /translation="MSDVRLTSFSHGAGCACKLSPADLGEVLGQTLAPQHPPEVLVAT
                     DTSDDAAVFRLPDGTALVQSVDFFTPVVDDAFDWGRIAAANALSDLYAMGARPVLALN
                     VVAWPVEDLPLDLLGEVLRGGEKVAAEAGAAIVGGHTIHDPGPKYGMAVTGLAALERV
                     TRNSTARPGADLVLTKPLGIGIATTAVKRDRAEPEALARAVEVMTTLNAAASEAVAEV
                     GAQAVTDVTGFGLLGHLHEMARGSGVGAEVDVAAVPLLPGTLELAAAGIVPGGTRKNH
                     RWIGPSVDWGDLPEVEQLVLADAQTSGGLLIAVDPERTDHLVAALERRDVRGARIGRT
                     ALGPAGRITVRGRLSEAR"
     gene            10555..10980
                     /locus_tag="VM638_07440"
     CDS             10555..10980
                     /locus_tag="VM638_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HVM12291.1"
                     /translation="MELPRRLFDRIVAHARAERPLEACGVVAGAGGAPVGVYPIANVE
                     RSPVRYRFDEREQRRVFREIEDRGWDLLAFFHSHPDSPAEPSSTDRMQAHRPDPLTGE
                     PVAVYPSTRYLILSLAGEPELRCFRFARGRAVEEEVRIS"
     gene            10977..11804
                     /gene="moeB"
                     /locus_tag="VM638_07445"
     CDS             10977..11804
                     /gene="moeB"
                     /locus_tag="VM638_07445"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HVM12292.1"
                     /translation="MSFTEEQVLRYARQMILPEIGGEGQARLARARVLVVGLGGLGSP
                     AATYLATAGVGTLGLVDHDRVDLSNLHRQPLHGTSDVGRAKVVSAAERLRELNPEVEL
                     EVHERALDAGNALEILAGYDVVVDGTDTFPARYLINDAAQLAGIPVAHGSVHRFEGQA
                     SVFLPGPETPCYRCLFPHPPEPDSVPSCAEAGVLGVLPGLVGTIQAAETIKLLTGVGE
                     TLAGRLLTIDALTMRFAGVAVRWDPGCAVCGRTPSLTELIDYDAFCGVVSASPSDAV"
     gene            11874..>12221
                     /locus_tag="VM638_07450"
     CDS             11874..>12221
                     /locus_tag="VM638_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HVM12293.1"
                     /translation="MSDRPIGVFDSGVGGLTVARAIIDLLPEEPILYFGDTARSPYGP
                     RPLEEIRGFAREIAAALLERDVKMLVVACNSVEVAAIADLTSATETPIVGVIEPGVRA
                     AMRVTRNGRIGVIG"
BASE COUNT         1834 a         4288 c         4438 g         1661 t
ORIGIN      
        1 aggcggccgc ccccacggcg acccaccgcg ccgacgccgc gccacggcgc cacgcctcga
       61 tcccgagcgc cgcgaggatc gggagcgcga gcaggacccc gtaacggaac cgtgacggct
      121 cgtgcacgta gaagtcggcg aacggcagcc aggcgcccca cgaggccacg gcggaggcca
      181 ccgcgcgaag cgggagcagg tagcaaagga gggcgaaggc accgatccag atcgcgaggc
      241 gccgacgttc ccgcagcgcc caggccgccg gcaccagcag gagcgccgcc atccccaggt
      301 acgcccctgg tgccgtggcg aacccgagga tccacggcgg ggagacggat gcgccgacct
      361 cggcgctcga cccgacgggg ctcccgaggt cggccgcgag cgcatcgagc ccgccgtagc
      421 cgagcgagag cgtcgagcgg gccaggtagg ggacacgcgg cgcgaggaac gcgaggttca
      481 cgagcacgag cgcggcgagg aggagcgccg cccgcagcca cgccacgcga gtcctcgggc
      541 gaacccgacc gccggcggcg atcgcgtacg ccgcgagcgc cagcgtcgcc acgacgagcc
      601 cgtggctgag gtgtgcggcg gccacctgcc cccacgcggc cgcgaccgcc accgaccaac
      661 cgagacgccc gctccacccc ggcgcccgga agaacctggc cgcgcaaccg agcatcacgg
      721 ccgtccacgc gaccgagccg gagaacggga gcgtcacggc gacgcgcgat gccgcgatgc
      781 cccccgccag cgcgaggccg ccgacggtgg ccgccggccg cgagagcccc tcgacgcgca
      841 ggaaccagta gagcccgacg cccgcgagga ccggctgcag ggtcacgaac cagccgaagg
      901 cggtcgtgca ggggagcgcc gcgtagagca gcatcgccgg caggtacatc cagccggact
      961 gcgggtccgc cgcgaacggg acgccgccga ggacgtgcgg gttccacgcc gggatcgagc
     1021 ccgaggcgag ggtctcgccg aggtggcagt gcatcgggag ccagaacggc agcgggtcga
     1081 cgtgctggag gctgacgcgc ccgaagagcc agaacccgcg gagcaggaag agagcgacgc
     1141 cggcgatcag gagcggtccg ccggcggccg cgagggcccg gcggagcctc ggggtcaccg
     1201 acccagcggc tgcggctcgg gcgccgcctc cgacacccgg tcgcccgcgg gaacccgcgc
     1261 cttgaagatc atctccgagc gcccgacgta gcggacgtcc tcgatcgcga gccccatgtc
     1321 ctcgagcagc ccgcggagct cgtcgtggtc gtagcggttg atgtgctcct tcacgtaccc
     1381 gccggggaag atcttcccgt agatccactc gagggtccgc cacagccgct tcccgtagtc
     1441 gggcgtcccg aggatcagcg cgcccccagg gcggatcacg cgcacgagct cctcgagccg
     1501 gacgtcctcc cgggggatgt gctcgatgac ctccgagttg acgaccgcgt cgaacgcgcc
     1561 gtcgcggaac ggcaggcggt tcatgtcgcc ctgcagcagc gggcgcccgg gcgcccggag
     1621 ccagcgcagc ttccgcaggg cgaggtccat cccgacgacc cgggggagcg tctgcacgat
     1681 gcgcgacgag ccgcacccga tgtcgagcac gcggccttcg cccgtcatgt agtcctggat
     1741 gatgcggaac cgggcccgct gccagtaccg ctggagcggg atccaggaat cgaaggcccg
     1801 atggtcgtag tcggcggcct tcacggagtt ccgcaggccg aacgccttcc cgagcgtccc
     1861 gaggtagccg aacccgagcc ggagcatccg catccgcgtg agcggccgcg cgccccggta
     1921 ccagaacggc acctcttcga tcttccatcc ctggcagtac gccttcacga tcatctcggg
     1981 caggatgtcg agccccttgc cgcggggcgg cccgatgtcg gcgaggaccg gcgcgcggaa
     2041 catccggaag ccgctcgaca ggtcccggta cggcagcgcg agggccttgc ggtagaggag
     2101 gttgagcgcc cggctgagcg accgccggag gaaccgcatc tcggggaagg cgcctcgcac
     2161 gtagcgcgag ccgatcagga cctcgccgcg ctcgcggtga tcccacatcg tgcggacgta
     2221 gcccgggcgg tgcgagaaat cggcatccat cgtcaggacg tgcgctccct cggccgcggc
     2281 gaagccggcg cggaggatct cgccgtaccc gcgtcccccc gccaacacga agcgcgcacc
     2341 cagcgcctcg acgcgcgccc ggagcgaggc gtcggcggag cagacgacga tctcatggtc
     2401 gtcgacggcg ccgcggaccg ccgagcggac ggcggggatc aggcgcggga ggttgtgctc
     2461 gcgcgcccag acggggatca cgacggagag gtcgggctgc ttcatcgcgc gtatcctacc
     2521 cggcgcccgg gcccggctac cggcgccggg tcgcggtata ggatgggccg acatggcgga
     2581 ccgcgcggag ctgatcgacc gactcgaagg gatcgcgcgc gagctccgca tcaccgatct
     2641 cgagatgatc gccggggccg gctccggcca cccgggcgga acgctctccg ccgccgacat
     2701 gatcgccgcg ctctacttcc acgtcctgcg cgtgcgtccc gaggagcccg agtggccgga
     2761 tcgcgaccgg ttcgtgctgt cgaagggcca ctgcatcccg atcgtctacg ccgcgatggc
     2821 caagcgcggg ttcttcgacg ccgagctgct caccaccctg cgcgatctcg ggtcgcccct
     2881 gcagggccac cccgaccgcc ggcggctgcc gggcgtggaa gcctccaccg ggtcgctcgg
     2941 gcagggcctg tcggtcgccg tcgggatggc gctcgcggcg aaggtcgacg gcgcctcgtg
     3001 gcgcgtcttc tgcatgctcg gcgacggcga gatccaggcg gggcaggtgt gggaggccgc
     3061 gatgttcgcc ccgaagcacg ggctcgacaa cctcatcgcg atccttgacc acaaccaggt
     3121 ccagcagacc gggcgcgtcg aggacatcct cgacgtcgcg ccggtcgcgg agaaatgggc
     3181 cgcgttcggg tggcacgtga tcgatatcga cggccacgac atgggccaga tcgtcgacgc
     3241 gctcgacgaa gccgacgggg cggaccgccc ggcgatcgtc gtctcgcgca cgaagaaggg
     3301 caagggcgtc tcctggatgg agctcaactc ggcctggcac ggcaaggcga cgaagccgga
     3361 ggagaccgag cgcgcgctcg ccgagctgcg agggcagggc tagtgggcgc ggcgacgcgc
     3421 gacgccttcg gcgaggcgat cctcgaggtc ggtgccgacg agcgcatcgt cgtgctgacc
     3481 ggggacctga aggactcgac ccgcaccgag gacttcgccg agaagtaccc ggagcggttc
     3541 ttcgacgctg gcatcgccga gtcgaacatg gtcgggatgg ccgccgggct cgcgctctcg
     3601 gggaagatcg cctgggcctg ctccttctcc tgcttcgtga ccggccggtt cgagacgatc
     3661 cgcgtctcgg tcgcgtacca ggaggcgaac gtgcggatcg tcggcacgca cagcgggatc
     3721 ggggtcggcg aggacggcta ctcccagatg gcgcaggagg acatcgcggc gctgcgtgcg
     3781 ctccccaaca tggcgatcgt caaccccgcg gacgcggccg agacgcgccg cgccgtccgt
     3841 gccctcgtcg agcacgaggg cccggcgttc ctccgtctga cgcggcaggg cgtcgacgac
     3901 gtgacggggg aggatggcgc cttcgacttc ggccgcatcg cgacgctccg cgacggcagc
     3961 gacgtcgcga tcgtcgggac gggagccacg gtcgccgagg ccctcaaggc cgccgacgcc
     4021 cttgccgccg agggagtctc ggcgcgggtg atgaacgcgc acacgctgaa gccgttcgac
     4081 gcggacgcga tcctcgcggc ggcggccgat tgcggcagga tcgtcacggt cgaggatcac
     4141 agtgtgcacg gtggcctcgg cagcgcggtc gcggaggtcc tcgcctcgga ggggagcccc
     4201 gcgcggctgc gggtgatcgg cacgacgacg ttcggcgaat cggggtcgcc cgcggcgctg
     4261 tacgagaagc acgggttgag cggcgcgcgc gtggcgcagg cgacccgcgg tttcctgaac
     4321 gcatcgacct gatccacccg gacgcgccgc aacacgcctg ctagggtgtt tcccatcgtt
     4381 ctgggcgtct gatagcatcc gcaacgacca cccccaaacc ctcccatgat caagaccgag
     4441 cacgtcacca aggcgtacaa ggactccgtc tatgccctcg acgacgtttc cgtcgagatc
     4501 ggcaagggcg agttcgtgtt catcgtcggc ccgtccgggt cggggaagtc gacgttcatc
     4561 cgcctgatgc tgaaggagga agagccgacg gcgggcaaca tctgggtcgc ggggaagaac
     4621 atcgcgaagc tgtcgccgtg gaaggtcccg tacctgcgtc gcaacatcgg cacggtcttc
     4681 caggacttca agctgctgcc ggaccgcacc gtcttcgaca acgtcgcctt cgcgctcgag
     4741 gtgatcggga agccccgcca tatcatcgac aagcgcgtgc ccgagatcct cgagctcgtc
     4801 ggcctcggcg acaagctcgg aaactacccc gacgagctct ccggcggcga gcagcagcgc
     4861 gtctccgtgg cgcgcgcgtt cgtcaaccgt ccgctcatcc tgctcgccga cgagccgacc
     4921 gggaacctcg acccgtcgac ctcggtcgac atcatgaagc tcctcgaccg catcaaccgg
     4981 accggcacga ccgtgatcat ggcgacgcac gacaacgcga tcgtcgacgc gatgcgcaag
     5041 cgcgtcatcg agctcgactc ggggaaggtc gtccgggacc aggctcgcgg ggtgtacggc
     5101 tacggtgcgt aggttccgct acttcttccg cgagacgttc acggcgctcc ggcgcaacgc
     5161 cctcgtcgcc ttcgccgcga tctcgacgat gttcatctcg ctcttcctgc tcggcggatc
     5221 gctcctggtc cagaagcagg tcgagctgct cgtcggggag tgggggtcga aggtcgaggt
     5281 ttcggtcttc ctccgcgagg acgcctcggg cgacgagatc caggcgctca cccgcgagat
     5341 cgagggcctg cccgaggtcc agcaggtctt cttcgagtcg aagcagcagg cctaccagcg
     5401 gttccagcgc ctgttccgcg actcacccgc gctcgtggag aacgtcgacc ccgagtcgat
     5461 gccgcagtcg ttccgcgtga agctcgtcga ccccgagcag ttctcggtga tccgcgcgcg
     5521 cctcgccgga cagccggcca tcgacgagat ccgcgatgag cagcgggtcc tcaagcgcct
     5581 cttcgcggtg accgccgtcc tgcggaccgg cgtcatgggc gtcgccttca tcatgctgat
     5641 cgcagcggcc gggctgatcg gcaacacggt gcggatggcg gtgttcgcac gccgcaagga
     5701 gatcgcgatc atgaagctcg tcggggcgac gaactggttc atccgtgtcc cgttcctcat
     5761 cgagggagcg gtcggcggcc tcctcggcgc cggcctcgcg gtgctcgggc tcttcgccat
     5821 gaagatgctg ttcatcgacc cgctgcgcgg caacatctcg ttcacgccgt gggtcgggac
     5881 cggagccgtg ctcgagaccg ttccgtggct cgtcggcgtc ggcatcctca tggccgtgat
     5941 cgcgagcctg ttcgcgatgc gccgcttcct cgaggtctga tcccgcagaa ggttgaagcc
     6001 gggcgaaacg cccggtagga tgttcacatc cccccaccga gcagccgagg ctctggcatg
     6061 cgcaggatcg ccctgatcat gaccctcgtg ctcgcggccc aggttcccgg gggcgcgttc
     6121 gtctccgcgc gtccgaacga cccgaggctc gagcgcatcg aggccgagat ccgccacaag
     6181 cgacagcaga tcaagcatgc tgaagcgcgg cgacagggcc tgctggagga gatctcgcgg
     6241 tccgacgcgc gtcgcgacga cctcacccgg gagatcgagg atctccaggc gtccctcgcg
     6301 gagacccgca cccggctcgc ggacacacgc gcctcgctcc gccaggcgaa ggatctgatc
     6361 gagcgctggt cgaggcgtct cgcggccgca gaggagtcgc tgctccagcg gcgcgaggac
     6421 ctcgggcggc gagcggcgaa cgcctacaag ctgggggccg ccggctacat ggacgtcatc
     6481 ctcggcgccg aggaccttcg ttcgctcacc gaccgcgtcc agttcgtcga cacggtcctg
     6541 ctggcggacg ccaccttcct gcgtgggctc gaggtcgcac gcgagctcgt cgaggagcgc
     6601 cggtcgaccg ttcgggagca ggagcgagac ctccgggccc aggagcgtcg cgtcgagcgc
     6661 gaggagcagc gcctcgagga gctgaaggcg gagcgtgagg cgctgcgcaa cgagatcgac
     6721 atggagatcg cgcaccgcca cgatctcgtc gaggggatcg agcagaacaa ggaggagtac
     6781 gagcgggcgg tccgcgagct cgaggcggag tccgcgtgga tcacggccca gctccgaggc
     6841 tcgggctcca ccggctcggg gcagatcggc gggaagctcc actggccgac cgccgggtcg
     6901 atcggctcgg gcttcggctg gcggacgcac ccgatcttcg gcacccgacg gttccacgcc
     6961 ggggtggaca tcggcgcccc ctgcggccag gcgatctatg cgggcgagag cggccgcgtg
     7021 atctcctccg gctggcaggg cgggtacggg cagacgatcg tgatcgatca cggcaggggc
     7081 ctctcgaccc tctacgccca tcagtcgagc ctcggggtcg ggagcggggc caccgtgcgc
     7141 cgcgcgcagc ggatcggctc ggtgggcacg acgggatggt ccaccgggtg ccacctgcac
     7201 ttcgaggtgc gggtgaacgg gaacccggtc gacccggtgc cctacctcac ctgaggctac
     7261 cctgagcccg tgaagggcca gcacaccgtc gcgacgaacc ggcgggcccg ccacgactac
     7321 gcgatcgacg agacgctcga ggccgggctc gtgctcaccg gcgccgaggt caagtcgctc
     7381 cgtgcggggc gggcctcgct cgcggacgcc tacggtcgcg tcgatggcgg cgaggtctgg
     7441 atcgaggggc tccacatccc gccctacgag cacgactcga gggaccgggg acgctacgag
     7501 gagaagcggc cgcgcaagct cctgctgcac cgagccgaga tcgatcggct cgtcgggaag
     7561 atcaaggagc gcgggatgac gctcgtgccg atgcggctgt acttcgtccg tggccgggcg
     7621 aagctcgagc tgggcctcgg tcgcgggaag cggcagtacg agaagcggcg gtccatcgcc
     7681 gagaaagagc agcgccgcga gatcgagcgc gcgctgtcgg cccgtcgccg cggctgaggg
     7741 atgtcggcgg ggggcgtata ctgatgggac aaccacatag ggggtgaact ggcttcgact
     7801 cgggcggtca acgccctggg ggaagcgagc cgagatcccg gatgtctcgt taaacgccgg
     7861 accgatacaa acgccgatga catggcgatc gctgcctgat caaggttagg tagccgctcc
     7921 ccgcggagcg cctgcgaccg cggatgagcg tcatcagcag gctcaccgac ccggagtcgc
     7981 ctcgcggccg ggtcgggaca ccgaagcgag gctgggccaa cggtgctccg cctgtgggag
     8041 ccggcggccg agacctcacc gcaggctacg ctcgtagaag tcccagggtg tccctcgagg
     8101 acgcgggttc gattcccgcc acctccacca tcacggatgc cccccgatcg tctacgcgct
     8161 tgcgcgtttc gggagggcgc tgaggtccag gtccatgcct tcgtgggcga ggaccggcgg
     8221 gttcggacgt tcgccccaga gctcgcgggc gcggtcgagc atgcgctcca gctcgtcgtc
     8281 ggtgtgcagg gggtcgtggt ggaacatcac gagctggccg acgtcggcga tccgagcgaa
     8341 ctgcaccacg tggtcgaggc tcgaatgtcc ccagccgacc ttccggaggt actcgtcctc
     8401 ggtgtactgg gagtcgtgga tcagcacgtc ggcgcccctc gcgacaccgt gtcccgagat
     8461 ccacgccggc tccacggccc gcaggtcgac cgcgaggcct ggctcgtggt cggggatgta
     8521 cgcgaggatc cgcccctccg actcgatccg gatcccgacc gtcggtccct ggtgcgagac
     8581 cggcatcgcc cgaaccacgg cctcctcgat ccccaggtcc ccgcgggcgt cgtggaaccg
     8641 gagtcggcac ggaacgtcct gaaggtggac cgggaagagc ggtggcgaga ggtaccgggc
     8701 gatgctctgc tccagcgtcc gcgtcggcga cggcgggccc cagatgtgga tctcgacgtc
     8761 cgggttccac agcggcccga agaacggcag cccctggagg tggtcgaggt gcaggtggct
     8821 gaggaacagg tggatcctgc gcggcacgtc ggcgccgagc gcgacgccga gcgggcggat
     8881 gccggttccg gcgtcgagga tcaggagcgt gtcgttcgcg aggcgcacct cgacgcagga
     8941 ggtgttcccg ccgtagcgca ccgtctcggg cccgggcgcc gcgagcgagc cgcgcgatcc
     9001 ccagatccgc gcgcgcacct acccctcggc ctcccagaag atcgccatcg cgccgatgaa
     9061 ctcatccgac ttcgcgaaca gcggaagcgc ggtcaccgcg atctctcgcg ccaccccgtc
     9121 ggcgccgacg atgtgcagcg gcccgtgcgc cgggcgtcgg tgggtgagcg cgatgccgag
     9181 cggcaggtcc tctggcggga tcggggtgcc gctccctggg tcgatggggc ggaaccgcgt
     9241 tccccattcc tcgagggtca tcgagccgga gtcggcgtag gactgaccga ggatgccctc
     9301 cgccggctcg ttgaagtaga cgagcgtccc ttcgggatcc acgaggaaca cgggcgtggc
     9361 gatgctcgac gcgagcccac gcgccaggat cagaacgagg ttgtgctcgt cggccatctc
     9421 gcctccaggg ccaatgatag ggcgggcgcg gcctcggcag gcgccggcgc tatcctcctg
     9481 gtatgagcga cgtccgactc acgagcttca gccacggcgc cggctgcgcg tgcaagctct
     9541 cgccggccga cctcggcgag gtgctgggcc agacgctcgc cccgcagcac ccgcccgagg
     9601 tgctggtcgc caccgacacg agcgacgacg cggccgtctt ccggctgccc gacggtaccg
     9661 cgctcgtgca gtcggtcgac ttcttcacgc cggtcgtcga cgacgccttc gactggggga
     9721 ggatcgcggc ggcgaacgcg ctgagcgacc tctacgcgat gggcgcgcgc cccgtgctgg
     9781 cgctcaacgt cgtcgcctgg ccggtcgagg accttccgct cgacctcctc ggcgaggtcc
     9841 tgcgcggtgg cgagaaggtc gcggccgagg ccggggcggc gatcgtcggc gggcacacga
     9901 tccacgaccc gggcccgaag tacggcatgg cggtcacggg gctcgctgct ctcgagcggg
     9961 tgacccgcaa ctcgacggct cgtccgggtg ccgacctcgt gctcacgaag ccgctcggca
    10021 tcgggatcgc gacgaccgcc gtcaagcgtg accgcgccga gccggaggcg ttggcgcggg
    10081 ccgtcgaggt gatgacgacc ctgaacgccg ccgcttccga ggccgtcgcg gaggtcggcg
    10141 cccaggccgt caccgacgtg accgggttcg ggctcctcgg ccacctccac gagatggctc
    10201 gcggctccgg tgtcggtgcg gaggtggacg tggccgcggt tccgctcctt cccgggacgc
    10261 tcgagctggc ggccgcgggg atcgtgccgg gcgggacccg gaagaaccac cggtggatcg
    10321 gtccgagcgt cgactggggc gacctgccgg aggtggagca gctggtcctc gccgacgccc
    10381 agacctcggg cggcctgctc atcgcggtgg accccgagcg gacggaccac ctggtggcgg
    10441 ccctcgagcg gcgggacgtc cggggggcgc gcatcggccg gacggcgctc gggccggccg
    10501 ggcgcatcac ggtgcgcggc aggctctccg aagcccgctg atagcatcga ggggatggag
    10561 cttccccgtc ggctcttcga ccgcatcgtc gcgcacgccc gcgcagagcg accgctcgag
    10621 gcgtgcggcg tcgtggcggg ggcgggcggc gcccccgtcg gcgtctaccc gatcgcgaac
    10681 gtcgagcgca gccccgttcg gtaccggttc gacgagcggg agcagcggcg cgtcttccgc
    10741 gagatcgagg accggggatg ggacctgctc gcgttcttcc actcccaccc ggattcgccg
    10801 gcggagccct cgtcgaccga tcggatgcag gcccaccggc cggatccgct gaccggcgag
    10861 cccgtggcgg tgtacccctc gacgcggtac ctgatcctgt cgctcgccgg ggagccggag
    10921 ctccgctgct tccggttcgc gcgcggccgg gcggtcgagg aggaggtccg gatctcgtga
    10981 gcttcaccga ggagcaggtc ctgcggtacg cccgacagat gatcctcccg gagatcggag
    11041 gcgagggtca ggcgaggctc gcgcgggcgc gggtgctggt ggttggcctc ggcggcctcg
    11101 gctcgccggc ggcgacctac ctcgcgacgg ccggcgtcgg caccctcggg ctcgtcgacc
    11161 acgatcgcgt cgacctctcg aacctccacc gccagccgct gcacggaacc tccgacgtgg
    11221 ggcgcgcgaa ggtcgtctcc gccgcggagc gtctccgcga gctgaacccg gaggtcgagc
    11281 tcgaggtcca cgagcgggcg ctcgatgccg ggaacgcgct cgagatcctc gccggctacg
    11341 acgtcgtcgt cgacgggacc gacacgttcc ccgcgcggta cctgatcaac gacgccgcac
    11401 agctcgccgg catccccgtc gcccacggct cggtccaccg gttcgagggc caggcgagcg
    11461 tcttcctgcc cgggcccgag acgccgtgct accgctgcct gttcccgcat cctcccgagc
    11521 cggacagcgt cccctcgtgc gccgaggctg gcgtcctcgg ggtccttccc ggcctggtcg
    11581 gcacgatcca ggccgcggag accatcaagc tcctgaccgg cgtcggggag acgctcgccg
    11641 gccggctgct gacgatcgac gcactgacga tgcgcttcgc cggggtcgcc gtgcggtggg
    11701 atcccggctg cgcggtctgc gggcgaacgc cgagcctcac cgagctgatc gactacgacg
    11761 cgttctgcgg ggtcgtctcc gcctcaccct ccgacgcggt ctgaggggtc ctggacgggg
    11821 tatcgtgggc cgttcgtcgg acggagacga ggtgtcgtag ggatgggcgg agcgtgagcg
    11881 atcggccgat cggggtcttc gactccggcg tgggcggcct caccgtcgca cgcgcgatca
    11941 tcgacctgct ccccgaggag ccgatcctgt acttcggtga caccgcccgc tcgccctacg
    12001 gcccgcgccc gctggaggag atccgcgggt tcgcccggga gatcgcggcc gcgcttctcg
    12061 agcgcgacgt gaagatgctg gtggtcgcgt gcaactccgt cgaggtcgcg gcgatcgcgg
    12121 acctcacgtc ggccacggag acgccgatcg tcggcgtgat cgagccgggt gtgcgggcgg
    12181 cgatgcgggt gacccgcaac ggccggatcg gggtgatcgg g
//
LOCUS       DATLGG010000259         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_152439, whole genome shotgun sequence.
ACCESSION   DATLGG010000259 DATLGG010000000
VERSION     DATLGG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152439"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VM638_07455"
     CDS             complement(<1..1331)
                     /locus_tag="VM638_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVM12294.1"
                     /translation="MSRKDEWRQRYRQAGERDADFETISGEPLEPLYTPEDLEGWDAE
                     EKLGYPGEYPFTRGVYPTMYRGRLWTMRQFAGFGTPKQTNERYKYLLKQGGGGLSVAF
                     DMPTLMGRDSDDPHSEGEVGRCGVATDSLADFETLFDGIPLGDITTSMTISGPAVILF
                     AFFLAAAEKQGADWKTLDGTLQTDILKEYIAQKEWLFPPRPHLRLIGDLMEFCAEEVP
                     KWHPISVSGYHIREAGSTAVQELAFTLADGFSYVELGKQRGIDVDRFSQGLSFFFNAH
                     IDFFEEIAKFRAARRIWARWMKERYGAKDPQSWRLRFHTQTAGCSLTAQQPENNIVRT
                     AIEALAGVLGGTQSLHTNALDEVLALPTEKAAQIALRTQQVIAYETGVTNVIDPLGGS
                     YFVEAFTDRTEAQAEEYFRKIDEMGNGSILEGMLVGIERGYFQSEIADAAYR"
     gene            complement(1328..2281)
                     /gene="meaB"
                     /locus_tag="VM638_07460"
     CDS             complement(1328..2281)
                     /gene="meaB"
                     /locus_tag="VM638_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HVM12295.1"
                     /translation="MELREVVEGVRGGDRRSVARAISMVEDGSDALPELIGGLFPDTG
                     RAYSIGLTGSPGVGKSSLAAELVKAARARDLRVAVLAIDPTSPFTGGALLGDRLRMQS
                     HATDPNVFIRSMATRGHLGGLALAAPEAVRVLDASGADVIVIETVGVGQAEVEVATQA
                     DTTLVVVAPGWGDAVQASKAGILEIADVFVVNKADREGAREAARDIENMLHLGPELDW
                     PPPVVKTATVTGEGIDDLWEAIERHRKHLESSGELDARRRKRLLHEVEGMVSARLRRQ
                     VAERLEEGDLERLADDLVARRVDPYAAADILVGTVTERRQG"
     gene            complement(2347..>2613)
                     /locus_tag="VM638_07465"
     CDS             complement(2347..>2613)
                     /locus_tag="VM638_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12296.1"
                     /translation="VRTRSGNRWVPLSEVTLEVFLDGESVGTVETDADGRTQVVVPGG
                     DPGEHVVKVRYAGDAEHRKAQRAQGFEVAEPDPGEEPELDGSEG"
BASE COUNT          362 a          969 c          864 g          418 t
ORIGIN      
        1 cggtaggcgg cgtcggcgat ctcggactgg aagtagccgc gctcgatgcc gacgagcatc
       61 ccttcgagga tcgagccgtt gcccatctcg tcgatcttgc ggaagtactc ctcggcctgc
      121 gcctcggtcc ggtccgtgaa ggcctcgacg aagtaggagc cgccgagcgg gtcgatcacg
      181 ttcgtgacgc cggtctcgta ggcgatcacc tgctgggtcc ggagcgcgat ctgcgccgcc
      241 ttctcggtcg gaagagccag cacctcgtcg agcgcgttcg tgtgcagcga ctgcgtcccg
      301 ccgagcaccc ccgcgagggc ctcgatcgcc gtccgcacga tgttgttctc cggctgctga
      361 gcggtgagcg agcatccggc ggtctgcgtg tggaagcgca gacgccacga ttgcgggtcc
      421 ttcgccccgt agcgctcctt catccagcgc gcccagatgc ggcgcgccgc gcggaacttc
      481 gcgatctctt cgaagaagtc gatgtgcgcg ttgaagaaga acgaaagccc ctgggagaag
      541 cggtcgacgt cgatcccgcg ctgcttgccg agctccacgt agctgaagcc gtcggcgagc
      601 gtgaacgcga gctcctgcac ggccgtcgac ccggcctcac ggatgtggta gccggagacg
      661 ctgatcggat gccacttcgg cacctcttcc gcgcagaact ccatcaggtc gccgatcagc
      721 cgcaggtggg gccgcggcgg gaacagccac tccttctgcg cgatgtactc cttgaggatg
      781 tccgtctgca aggtgccgtc gagggtcttc cagtccgcgc cctgcttctc ggcggcggcg
      841 aggaagaagg cgaagaggat caccgccggg ccgctgatcg tcatcgaggt cgtgatgtcg
      901 ccgagcggga tcccgtcgaa gagggtctcg aagtcggcga gcgagtcggt ggcgacgccg
      961 cagcggccga cctcgccctc gctgtgcggg tcgtcggagt cgcggcccat cagcgtcggc
     1021 atgtcgaagg cgacggacag cccgcccccg ccctgcttca gcaggtactt gtagcgctcg
     1081 ttcgtctgct tcggcgtccc gaagccggcg aactgccgca tcgtccagag gcgcccgcgg
     1141 tacatcgtcg ggtacacgcc gcgggtgaag gggtattcgc ccgggtagcc gagcttctcc
     1201 tccgcgtccc agccctcgag gtcctcgggc gtgtagagcg gctccagggg ctcgccggag
     1261 atcgtctcga agtccgcgtc ccgctccccc gcctgccggt agcgctggcg ccactcgtcc
     1321 ttgcggctca tccctgcctc cgttcggtga ccgtgcccac caggatatcc gccgcggcgt
     1381 aggggtccac ccgtcgggcg accaggtcgt cggccaggcg ctcgaggtcg ccctcttcca
     1441 gacgctccgc gacctgccgc cgcagcctcg ccgagaccat cccctcgacc tcgtgcagca
     1501 atcgcttgcg ccgtcgcgcg tcgagctcgc ccgatgactc gaggtgcttg cggtgccgct
     1561 cgatcgcctc ccacaggtcg tcgatcccct cccccgtgac ggtcgccgtt ttcacgaccg
     1621 ggggcggcca gtcgagctcg ggaccgaggt ggagcatgtt ctcgatgtcg cgcgccgcct
     1681 cgcgggcgcc ctcgcggtcg gccttgttca cgacgaagac gtcggcgatc tcgaggatcc
     1741 cggccttcga cgcctgcacc gcgtcgcccc agcccggcgc caccacgacg agcgtcgtgt
     1801 ccgcctgggt cgcgacctca acctccgcct gccccacacc gaccgtctcg atcacgatca
     1861 cgtccgcccc cgatgcatcc aggacccgga cggcctccgg cgcggcgaga gccagaccgc
     1921 cgaggtggcc gcgcgtggcc atcgagcgga tgaacacgtt cgggtccgtc gcgtgcgact
     1981 gcatccgcag gcggtcgccg agcagcgccc cgccggtgaa cggtgaggtc gggtcgatcg
     2041 ccagcacggc cacgcgcagg tcgcgcgcgc gtgccgcctt caccagctcg gccgcgaggc
     2101 tcgatttccc gacaccgggc gagcccgtga gcccaatcga gtaggcacgg ccggtgtccg
     2161 ggaacagccc gccgatcagc tcgggaaggg cgtcggagcc atcctcgacc atcgagatgg
     2221 ctcgagcgac ggaccggcga tcaccgcccc gtaccccctc gacgacctcc cgaagctcca
     2281 cgcgccacag cgtagcagtc gcccccgggg ggacccgcgg gggcgaccac gtgcagcgtc
     2341 cgggggctat ccctccgacc cgtcgagctc tggctcctct ccggggtcgg gttctgccac
     2401 ctcgaagccc tgggcgcgct gtgccttcct gtgctcggcg tcgcccgcat acctgacctt
     2461 gacgacgtgc tcaccgggat ctccgccggg gacgaccacc tgcgtgcggc catccgcatc
     2521 cgtctccacg gttccgacgc tctcgccgtc gaggaacacc tcgagcgtca cctccgacag
     2581 cggcacccag cggttcccgc tccgcgtccg gac
//
LOCUS       DATLGG010000260         6615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_120860, whole genome shotgun sequence.
ACCESSION   DATLGG010000260 DATLGG010000000
VERSION     DATLGG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120860"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VM638_07470"
     CDS             <1..252
                     /locus_tag="VM638_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HVM12297.1"
                     /translation="TCILVDDVLYTGRTIRAAMDALVDFGRPRAIRLAVLVDRGHREL
                     PIRADHVGKNLPTSQDQRVQVRLEETDGEDAVEVGEGAG"
     gene            249..1169
                     /locus_tag="VM638_07475"
     CDS             249..1169
                     /locus_tag="VM638_07475"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVM12298.1"
                     /translation="MKHLLSATQLSAEEITRILDTADSFREVGTRVIKKVPALRGRTV
                     VNLFLENSTRTRISFEVAAKRLSADVINFSAGGSSVAKGESLKDTALTLQAMGADAIV
                     IRHSSSGAPYTLTRWVDASVLNAGDGTHEHPTQALLDLYTIREHFPSFEGLRVAIVGD
                     ILHSRVARSGTHALVAMGAEVTLVGPPTLIPPAAATWGADISHDLDAVLPKLDVCYML
                     RVQRERQRQQYFPSVREYSRRFGLTRERVAALPEQALIMHPGPMNRGVEIDSDVADLP
                     RSLITDQVTNGIAVRMALLYLILGAGEAER"
     gene            1169..2464
                     /locus_tag="VM638_07480"
     CDS             1169..2464
                     /locus_tag="VM638_07480"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVM12299.1"
                     /translation="MGRLLLRGGRLVDPASGHDAEADVLLEDSLIAAVGEGIGAAGAE
                     VLDCGGLVVAPGLVDLHAHFREPGLEHKETVETGSRAAAAGGYTAVCTMPNTEPVTDH
                     LAIVLEVRALGEKAGLCDVLPSAAITKGLEGESLVEIGELAAAGVRLFTDDHRSVTSS
                     RMMRVALEYARAFDVVICQHAQDEELSEGWQMHEGQYSALLGLRGAPREAEEVTVARD
                     LLLARLTGGRLHLTHLSTAGAVEAVRRARAEGSRVTADVTPHHLSLIDEDLLDYDTNR
                     KMNPPLRTAEDRDALRAGLADGALDAVATDHAPHAVEEKEREFDQAPPGTTGLETALS
                     AVLTNAVEPGHVSLARAIEAMSTAPARILGLEDHGGPVAAGRAANLVVFDPAAEWVVE
                     ERFHSRGRNSAFLGERLRGRVIHTILRGELTVRNGEPTR"
     gene            2461..3594
                     /gene="carA"
                     /locus_tag="VM638_07485"
     CDS             2461..3594
                     /gene="carA"
                     /locus_tag="VM638_07485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HVM12300.1"
                     /translation="MTRALLCLEDGTWFEGRGFGAGGERFGEIVFNTAMSGYQEVLTD
                     PSYASQIVVMTSPHQGNYGVNDEDAESDRIQVAGFAVREAARRSSSWRAERSLADELE
                     AAGVVGIEDLDTRALTLHLRDRGALRGAISTEDLDPASLAERVRARPGMEGAELARSV
                     STTAPYAAADLVGPAVPRADGRTFRVAAYDWGLKRHILRCLAAAGCEVTVLPAEAPAE
                     EALDGFDGVFLSNGPGDPAATTYAVATVRRLLGRVPVFGICLGHQILSLAVGGRTYKL
                     KFGHRGANQPVRDDDTGVVEVTSHNHGFAVDPDGWPRRDRVAETTHGPAALTHWNLND
                     GTLEGLRLLDVPAFAVQYHPEAAPGPHDSRSLFDRFVTLMEAS"
     gene            3591..4097
                     /locus_tag="VM638_07490"
     CDS             3591..4097
                     /locus_tag="VM638_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12301.1"
                     /translation="MKAKVIAAFVAVAMIGGAVAGVLALFGADDEPRPEALTAEEVGI
                     LAMHTLQRATAEQMRHFVSEGVFFDDPARLAPVAEEGGLFLVSTLEVCERRTVAVLGT
                     DTPDGRTFAVRVQGLEPPEDGEAEIGHFTSVPACEAAGGPGSWPGGYRITREGLMKGD
                     AVVDTAVS"
     gene            4069..>6615
                     /gene="carB"
                     /locus_tag="VM638_07495"
     CDS             4069..>6615
                     /gene="carB"
                     /locus_tag="VM638_07495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HVM12302.1"
                     /translation="MPLSTPPSADLRSVLVIGSGPIVIGQACEFDYSGTQACKVLRRE
                     GIRVVLVNSNPATIMTDPELADRTYVEPITPESVLRVIERERPDALLPTLGGQTALNV
                     AVALAETGELDRHGVRLIGADLEAIRRAEDRRLFVKTMTEAGLPLPEAGFAHSVEQAL
                     EVGDRLGYPVMVRPSFVLGGGGTGLARDRASLATVAAEGIAASPIGEIIVEESVEGWK
                     EFELEVMRDRADNAVIVCSIENVDPMGVHTGDSVTVAPQQTLSDREYQRMRDTALAVI
                     RAVGVETGGSNIQFAVDPETGRQVVIEMNPRVSRSSALASKATGFPIAKIAAMLAIGY
                     RLDEITNDITGETPACFEPALDYVVVKVPRWAFEKFPGADQTLTTTMKSVGEVMAAGR
                     SFKEALGKALRSLETGADVLAEREPPADLLRKLATPSEGRLPLVEAALRAGLTVEEVA
                     AASRIDPWFVDQIAQMGEVAEALRGRRLARLDEDHLREAKRAGLSDRRVAAILRTGER
                     EVRAARRAAGVEPVYKTVDTCAGEFAAKTPYHYSTYERVSEVSRGAGRRVVIVGAGPN
                     RIGQGIEFDYACVHAAYALREAGYETVMVNSNPETVSTDYDTSDRLYFEPLTAEDVLS
                     VCEAEEPAGVIVQLGGQTPLRLARELESEGFPILGTPPSAIDLAEDRGKFGALLTRLG
                     VAHPPHGEASSEDEAIEVAERVGYPVVVRPSYVLGGRAMEIVYTPEELAGYVRTATEA
                     APEHPVFIDRFLEGAIEVDVDAVCDPDEVLIGAVMEHIEEAGVHSGDSSCTIPPVTLS
                     DEELDEVERVTRVIARELGVIGLLNLQLAVKDERVFVIEANPRASRTVPFV"
BASE COUNT          931 a         2372 c         2410 g          902 t
ORIGIN      
        1 acctgcatcc tcgtcgacga cgtcctctac accggccgga cgatccgagc cgcgatggac
       61 gccctcgtcg atttcggacg tccccgtgcg atccggctcg cggtcctcgt ggaccggggg
      121 caccgcgagc tcccgatccg cgccgaccac gtcggcaaga acctcccgac aagccaggac
      181 cagcgggtgc aggtccggct cgaggagacc gacggcgagg acgccgtcga ggtgggggag
      241 ggagcgggat gaagcacctc ctgtctgcga cccagctctc ggccgaggag atcacccgca
      301 tcctcgacac cgccgactcc ttccgcgagg tcggcacccg cgtgatcaag aaggtgccgg
      361 cgctgcgcgg gcgaacggtc gtcaacctct tcctggagaa ctccacccgg acccggatct
      421 cgttcgaggt cgcggccaag cggctgtcgg cggacgtgat caacttctcc gcgggcggct
      481 cgtcggtcgc gaagggcgag agcctgaagg acacggcgct caccctccag gccatgggcg
      541 ccgacgcgat cgtgatccgc cattcctcgt cgggcgcccc ctacaccctc acgcgatggg
      601 tcgacgcgag cgtgctcaac gcgggcgacg ggacgcacga gcacccgacc caggcgctcc
      661 tcgacctcta cacgatccgc gagcacttcc cgagcttcga ggggctccgc gtggcgatcg
      721 tcggcgacat cctgcactcc cgcgtcgctc gctccggaac ccacgcgctc gtcgcgatgg
      781 gcgccgaggt gacgctcgtg ggcccaccga cgttgatccc gccggcggcc gcgacctggg
      841 gcgccgatat ctcgcacgac ctggacgccg tcctgccgaa gctcgacgtc tgctacatgc
      901 tgcgggtcca gcgcgagcgt cagcgccagc agtacttccc gagcgtgcgt gagtactccc
      961 gccgcttcgg cctcacgcgc gaacgggtcg cggcgctccc cgagcaggcg ctgatcatgc
     1021 acccggggcc gatgaaccgc ggggtcgaga tcgactcaga cgtcgccgac ctcccgcgct
     1081 ccctcatcac cgatcaggtg acgaacggga tcgccgtccg gatggcgctc ctgtacctga
     1141 tcctcggcgc tggggaggcg gagcgctgat gggccggctc ctcctgcgcg gcggccggct
     1201 cgtcgacccg gcgtctgggc acgacgccga ggcggacgtc ctgctcgagg actcgctgat
     1261 cgctgcggtc ggcgaaggga tcggggcggc cggcgccgag gtcctggact gcggcggcct
     1321 cgtcgtcgcg ccggggctcg tcgacctcca cgcccacttc cgggagccgg gactcgagca
     1381 caaggagacg gtcgagacgg ggtcccgcgc ggccgccgcc ggtgggtaca cggccgtgtg
     1441 cacgatgccg aacaccgagc cggtgaccga ccacctggcc atcgtcctcg aggtccgcgc
     1501 gctcggcgag aaggccggac tctgcgacgt cctcccctcg gccgcgatca cgaaggggct
     1561 cgagggggag tcgctcgtcg agatcggcga gctcgccgcc gccggggtgc gcctgttcac
     1621 cgacgatcat cggtcggtca cctcgtcgcg gatgatgcgg gtggccctcg aatacgcgcg
     1681 cgcgttcgac gtcgtgatct gtcagcacgc ccaggacgag gagctgagcg agggctggca
     1741 gatgcacgag gggcagtact cggcgctgct cggtctgcgc ggcgcccctc gggaggcgga
     1801 ggaggtcacg gtggcgcgcg acctcctgct cgcgcgtctg acgggcggac ggctgcacct
     1861 gacgcacctg tccaccgccg gcgcggtcga ggccgtccgg cgggcgcgcg ccgagggctc
     1921 ccgcgtgacg gccgacgtca cgccgcacca cctctcgctg atcgacgagg acctcctcga
     1981 ctacgacacg aaccgcaaga tgaacccccc gctgcggacc gccgaggacc gcgacgccct
     2041 ccgcgccggg ctcgccgatg gggcgctcga cgccgtcgcc accgaccatg cgccgcacgc
     2101 cgtcgaggag aaggagcggg agttcgacca ggcgcccccc gggacgaccg gcctcgagac
     2161 ggcgctctcg gcggtcctga cgaacgccgt cgagcccggc cacgtctcct tggcccgggc
     2221 gatcgaggcg atgagcacgg cgccggcgcg gatcctcggt ctggaggacc acggcggtcc
     2281 ggtcgcggcc ggtcgtgccg cgaacctcgt cgtgttcgac cccgcggccg agtgggtcgt
     2341 cgaggagcgc ttccactcgc gcgggcggaa cagcgccttc ctgggggaga ggctccgcgg
     2401 ccgggtcatc cacacgatcc tgcgggggga gctcaccgtc cggaacggag agccgacgcg
     2461 gtgacgcgcg cgctcctctg cctcgaggac ggcacctggt tcgagggacg cggattcggg
     2521 gccggcggcg agcgcttcgg ggagatcgtc ttcaacaccg cgatgtcggg ctaccaggag
     2581 gtgctgaccg acccgtcgta cgcctcccag atcgtcgtca tgacctctcc ccaccagggc
     2641 aactacggcg tgaacgacga ggacgccgag tccgaccgga tccaggtggc cggcttcgcg
     2701 gtccgcgagg cggcccgccg ctccagctcc tggcgggcgg agcgctcgct cgccgacgag
     2761 ctcgaggcgg cgggcgtcgt cgggatcgag gacctcgaca cccgggcgct caccttgcac
     2821 ctgcgcgacc gaggcgcgct gcgcggcgcg atctccaccg aggacctgga ccccgcgtcc
     2881 ctggcggagc gggtccgcgc gcggccgggg atggaggggg ccgagctcgc gcgcagcgtc
     2941 tcgacgaccg cgccctacgc ggcggcggac ctcgtcggcc ccgcggtccc acgcgcggac
     3001 ggccggacct tccgcgtcgc cgcgtacgac tggggcctca agcgccacat cctccggtgc
     3061 ctcgccgccg ccggctgcga ggtgacggtc ctgcccgccg aggcgccggc cgaggaggcg
     3121 ctcgacggct tcgacggcgt cttcctctcg aacggccccg gcgaccccgc cgcgacgacc
     3181 tacgccgtcg ccaccgtccg gcggctcctc ggccgggttc cggtgttcgg gatctgcctc
     3241 gggcaccaga tcctgagcct cgccgtgggc gggcggacct acaagctgaa gttcgggcat
     3301 cgcggggcga accagccggt ccgcgacgac gacaccggcg tcgtggaggt gacgagccac
     3361 aaccacgggt tcgccgtcga ccccgacggc tggccgcgcc gcgatcgagt ggccgagacg
     3421 acgcacgggc ccgcggcgct cacgcactgg aacctcaacg atgggacgct cgaggggctg
     3481 cggctgctcg atgtccccgc cttcgccgtc cagtaccacc ctgaggccgc gcccggtccc
     3541 cacgactcgc gctccctctt cgaccgcttc gtcacgctca tggaggcgtc gtgaaggcga
     3601 aggtgatcgc ggcgttcgtc gcggtcgcga tgatcggcgg cgccgtcgcc ggggtcctgg
     3661 cgctgttcgg cgccgacgac gagccccggc cggaggcgct gacggcagag gaggtcggga
     3721 tcctggcgat gcacacgctc cagcgggcga cggccgagca gatgcgccac ttcgtgagcg
     3781 agggcgtctt cttcgacgac ccggcgcggc tcgctcccgt ggccgaggag ggcggcctct
     3841 tcctcgtctc gaccctcgag gtctgcgagc gacggacggt cgccgtgctc gggacggaca
     3901 cgcccgacgg gcggacgttc gcggtccggg tgcagggcct cgagccgccg gaggacggcg
     3961 aggccgagat cggtcacttc acgagcgttc ccgcgtgcga ggccgcgggt gggccggggt
     4021 cgtggccggg cgggtaccgc atcacgcgcg aaggactgat gaagggagat gccgttgtcg
     4081 acaccgccgt cagctgacct ccggtcggtg ctcgtgatcg gctccgggcc gatcgtgatc
     4141 ggccaggcct gtgagttcga ctattccggc acccaggcgt gcaaggtcct gcggcgagag
     4201 gggatccggg tcgtgctcgt gaactcgaac ccggccacga tcatgacgga ccccgagctc
     4261 gccgatcgca cctacgtcga gccgatcacc ccggagtcgg tcctgcgcgt catcgagcgc
     4321 gagcggccgg acgcgctgct tccgaccctc ggggggcaga ccgcgctgaa cgtggcggtc
     4381 gccctcgccg agacggggga gctcgaccgc cacggcgtcc ggctgatcgg cgccgacctc
     4441 gaggcgatcc gccgcgccga ggaccgccgc ctgttcgtga agacgatgac ggaggccggc
     4501 cttcccctgc cggaggcggg gttcgcccac tcggtcgagc aggccctcga ggtgggcgac
     4561 cggctcgggt acccggtgat ggtccggccg tccttcgtgc tcggcggcgg cgggacgggg
     4621 ctggcccggg accgcgcgtc gctcgcgacg gtcgccgccg aagggatcgc ggcgagcccg
     4681 atcggcgaga tcatcgtcga ggagtcggtc gagggctgga aggagttcga gctcgaggtg
     4741 atgcgcgacc gcgccgacaa cgcggtgatc gtgtgctcga tcgagaacgt ggatccgatg
     4801 ggtgtccaca ccggcgactc ggtcaccgtc gcccctcagc agaccctgag cgaccgggag
     4861 taccagcgga tgcgcgacac ggcgctggcc gtgatccgcg cggtcggggt ggagaccggc
     4921 gggtcgaaca tccagttcgc ggtcgatccc gagacgggcc ggcaggtcgt gatcgagatg
     4981 aaccctcggg tctcccgctc gagcgcgctg gcgagcaagg cgacgggctt cccgatcgcg
     5041 aagatcgccg cgatgctcgc gatcggatac cgcctcgacg agatcacgaa cgacatcacg
     5101 ggcgagaccc ccgcctgctt cgaacccgcg ctcgactacg tcgtcgtcaa ggtcccccgc
     5161 tgggccttcg agaagttccc cggcgccgac cagaccctca cgacgacgat gaagtcggtg
     5221 ggggaggtga tggcggccgg ccgttcgttc aaggaagcgc tcggcaaggc gctgcgctcc
     5281 ctcgagaccg gagcggacgt cctcgccgaa cgggagccgc ccgcggacct gctccggaag
     5341 ctggcgacgc cgagcgaggg ccgtctcccg ctcgtcgagg cggcgttgcg cgccgggctg
     5401 accgtcgagg aggtcgccgc ggcatcccgg atcgacccct ggttcgtcga tcagatcgcg
     5461 cagatgggcg aggtggccga ggcgctgcgc ggacgccgtc tcgcgcgcct cgacgaagac
     5521 caccttcggg aggcgaaacg agcgggtctc tccgaccgtc gtgtggcggc gatcctccgc
     5581 acgggggagc gcgaggtccg cgccgcgcgc cgcgccgcgg gcgtcgagcc cgtctacaag
     5641 accgtcgaca cgtgcgccgg tgagttcgcg gcgaagacgc cgtaccacta ttcgacgtac
     5701 gaacgcgtgt cggaggtctc ccgcggcgcc ggtcgccggg tcgtcatcgt cggcgcgggc
     5761 ccgaaccgca tcgggcaggg gatcgagttc gactacgcgt gcgtgcacgc cgcctacgcc
     5821 ctccgagaag cgggctacga gaccgtgatg gtgaactcga accccgagac cgtctcaacg
     5881 gactacgaca cgagcgaccg cctgtacttc gagccgctca ccgcggagga cgtgctctcg
     5941 gtctgcgagg cggaagagcc ggccggggtg atcgtgcagc tcggcggcca gacgccgctt
     6001 cggctcgcgc gcgagctgga gtcggagggg ttcccgatcc tcggcacgcc tccctccgcg
     6061 atcgacctcg cggaggaccg cgggaagttc ggagcgctcc tgacgcggct cggcgtcgcg
     6121 catccgccgc acggcgaggc gtcgagcgag gacgaggcga tcgaggtcgc ggagcgggtc
     6181 gggtacccgg tcgtcgtgcg cccgtcctac gtcctcggcg gtcgcgcgat ggagatcgtg
     6241 tacaccccgg aggagctcgc cggctacgtc cgcacggcca cggaagccgc ccccgagcat
     6301 ccggtcttca tcgaccgctt cctcgaggga gcgatcgagg tcgacgtcga cgccgtctgc
     6361 gatcccgacg aggtcctgat cggagcggtg atggagcaca tcgaggaggc cggcgtccac
     6421 tcgggcgact cctcctgcac gatccccccg gtcacgctct cggacgagga gctcgacgag
     6481 gtcgagcggg tgacgcgggt gatcgctcgg gagctcggcg tgatcggcct gctgaacctc
     6541 cagctcgcgg tgaaggacga gcgggtcttc gtgatcgagg ccaacccgcg cgcgtcgcgg
     6601 acggtgccct tcgtc
//
LOCUS       DATLGG010000261         4321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_214415, whole genome shotgun sequence.
ACCESSION   DATLGG010000261 DATLGG010000000
VERSION     DATLGG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214415"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VM638_07500"
     CDS             <1..1443
                     /locus_tag="VM638_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HVM12303.1"
                     /translation="EHGRNGWFHVVLWPGDRQRLVDEGFTFEILVEDLVAQDIADRTA
                     ELEAQRAAPQERLMYRMLSDYENDLQKLAQENPSFVRHFTLTGTTHEGRRIHGVEIAK
                     DVTRAATDGRPTSLICGLHHAREWPSAELTMMLAEDIVDEYKKGTARYVNLVDNVRII
                     LLPVVNPDGFVRSRETVSDFIYPHPIVVQEFAYHRKNMRKNTTALVETGQGVDTNRNY
                     PYMWGGTGSSGTASSQTYRGANPGSEPEVVGLLNLFRSNQVLTMITNHTYSDLVLRPW
                     GHKAADPPDEAHLRALGNAMAAINGYTSQKGIQLYKTNGITEEWVYGAMGGYGYTFEI
                     GSSQFHPQYNTTIPGYWSRNRDAFLLCLEAARNTAYHSVLRGAFPPGTTVGISKAVTI
                     PLDPTGGRGTTYQDAMSSSIVVGDGGSFEWHVNPSPLPETVDQVNKGGLPESALEPYR
                     VAVTTPDGVTRTKEIVVLRGRTYEVSFDEE"
     gene            complement(1456..1698)
                     /locus_tag="VM638_07505"
     CDS             complement(1456..1698)
                     /locus_tag="VM638_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HVM12304.1"
                     /translation="MADITMYGADWCGDCRRAKRFFDERGIAYRYIDVEHDREMAEEA
                     ARIGRSRRIPVVVLPDGAVLVEPTNPELEARLFPVG"
     gene            complement(1749..3833)
                     /gene="fusA"
                     /locus_tag="VM638_07510"
     CDS             complement(1749..3833)
                     /gene="fusA"
                     /locus_tag="VM638_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVM12305.1"
                     /translation="MKTYEPTAIRNVLLVGHGGSGKTTLTEALLYTAGAITRMGRIED
                     GNTVSDFEPEETKKQISVSLSLAPIEWKGVKINLLDAPGYADFIGDVKVALRAADVCL
                     FVVSAVDGVEVQHEMVWELAAEARVPRAIFINKLDRERASFQRTLDELVTAFGTQVAP
                     VQFPIGEEHDLEGVADLLTRKAYRYPDGPAGAEGEWPDDIAGKADPYREKLAEAVAEA
                     DDTLLEKYLETGELSAGEIVTGVKAGLVQAKFAPVLCGSASKAVGVDRLAEFMREAFP
                     APTDRTPVKVMTRDGEEQERAPDPNGPLTGFVFKTISDPYVGRISLFRVYSGRLRADS
                     TVHNATQNEDERVSQLFTMRGKEHENVPEVVAGDIGAVAKLGHTHTGDTLTAKDGAAT
                     LPAVEWPEPLFSVAIEPKTQGDEEKLSTALARLKEEDPTFLVERNPETHQTVASGLGE
                     AHLDVMTERMRRKFGVDVGTVPARVPYKETLRQKVQALGRHVKQSGGHGQYGIAHVEV
                     EPLERGGGFDFVNKIVGGVIPTQFIPSVEKGIVKAMESGLTGHQVVDIRVTLYDGKYH
                     TVDSSDMAFQIAGSLAIKDALEKGGVVLLEPIVEAAVTVPESLTGDVIGDLNAKRGRV
                     LGMEPTGTGKSVVRAEVPKAEMMRYAIDLRSITGGRGVFTMKFSHYEEVPQHLADKVI
                     AEARREKEEAHK"
     gene            3916..4296
                     /locus_tag="VM638_07515"
     CDS             3916..4296
                     /locus_tag="VM638_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12306.1"
                     /translation="MTERLDVPSGRPAFPLSLLVAAVAFVALGAWFALDGRDPAAGVA
                     VLVGLALLVGGGRSARAMGSPGVMFTDSAVERICDAALLAAIAWSRLDVAPRVATAAL
                     IALVTSYLASYLRAKSTGLGFARR"
BASE COUNT          627 a         1495 c         1499 g          700 t
ORIGIN      
        1 gagcatggcc ggaacggatg gttccacgtc gtcctgtggc cgggggatcg gcagcggctc
       61 gtcgacgagg gattcacgtt cgagatcctg gtcgaggacc tcgtggccca ggacatcgca
      121 gaccgcacgg ccgagctcga ggcgcagcga gccgcgccgc aggagcggct gatgtaccgg
      181 atgctctcgg actacgagaa cgacctccag aagctggcgc aggagaaccc ctccttcgtg
      241 cggcacttca cgctcacggg gacgacccac gaggggcgac ggatccacgg cgtcgagatc
      301 gcgaaggatg tgacgcgggc ggccaccgac gggcggccga cctcgctcat ctgcgggctg
      361 catcatgccc gcgagtggcc gagcgcggag ctcacgatga tgttggccga ggacatcgtg
      421 gacgagtaca agaagggcac cgctcgctac gtgaacctcg tcgacaacgt gcggatcatc
      481 ctcctccccg tcgtgaaccc cgatggcttc gtgcgctcac gcgagacggt ctcggacttc
      541 atctacccgc acccgatcgt cgtgcaggag ttcgcgtacc accggaagaa catgcgcaag
      601 aacacgaccg ccctggtgga gaccgggcag ggcgtcgaca cgaaccgcaa ctatccctac
      661 atgtggggcg ggaccgggtc gagcggcacg gccagcagtc agacctatcg cggcgcgaac
      721 cccggcagcg agcccgaggt ggtcgggctg ctgaacctct tccgctcgaa ccaggtgctc
      781 acgatgatca cgaaccacac ctactccgac ctggtgctgc ggccgtgggg ccacaaggcg
      841 gcggacccgc cggacgaggc gcacctgcgc gccctcggca acgcgatggc ggcgatcaac
      901 gggtacacgt cgcagaaggg gatccagctc tacaagacga acggcatcac tgaggagtgg
      961 gtctacgggg cgatgggcgg ctacggctac accttcgaga tcgggtcgtc gcagttccat
     1021 ccgcagtaca acacgaccat cccgggctac tggagccgga accgagatgc cttcctcctc
     1081 tgcctggagg cagcccgcaa caccgcctac cactccgtcc tgcgcggggc cttccccccg
     1141 gggacgaccg tggggatcag caaggccgtc acgatcccgc tcgacccgac cggggggcga
     1201 gggacgacct accaggacgc gatgtcctcg tcgatcgtcg tgggcgacgg cggatcgttc
     1261 gagtggcacg tgaacccgag cccgctgccg gagacggtcg accaggtcaa caagggtggc
     1321 ctgccggagt cggcgctcga gccgtaccgg gtcgccgtga cgacgcccga cggcgtcacg
     1381 cgcacgaagg agatcgtggt cctgcgtggg aggacgtacg aggtctcgtt cgacgaggag
     1441 tagaagggga agggctcagc cgaccgggaa gaggcgcgct tcgagctcgg ggttcgtcgg
     1501 ctccacgagc acggcgccgt cggggagcac gacgaccggg atccggcgcg accggccgat
     1561 gcgcgccgcc tcctcggcca tctcgcggtc gtgctcgacg tcgatgtagc ggtaggcgat
     1621 gccgcgctcg tcgaagaacc gcttcgcgcg gcggcagtcg ccgcaccagt ccgccccgta
     1681 catcgtgatg tcggccatcg catccatcct acccgcgcgg gtggtgttcg ctgcgggtgc
     1741 cgacgctcct acttgtgcgc ctcttccttc tcccggcggg cctccgcgat gaccttgtcc
     1801 gcgaggtgct gagggacctc ctcgtagtgg gagaacttca tcgtgaagac cccgcgcccc
     1861 ccggtgatcg accgcaggtc gatcgcgtag cgcatcatct cggccttggg gacctcggct
     1921 cggacgacgc tcttgcccgt cccggtcggc tccatcccaa gcacccgccc ccgcttcgcg
     1981 ttcaggtcgc cgatcacgtc gccggtgagg gactccggga cggtgaccgc ggcctcgacg
     2041 atcggctcga gcaggacgac gccgcccttc tcgagcgcgt ctttgatcgc gagcgacccc
     2101 gcgatctgga acgccatgtc ggaggagtcg acggtgtggt acttcccgtc gtagagcgtg
     2161 acgcggatat cgacgacctg gtggccggtg agccccgatt ccatcgcctt gacgatgccc
     2221 ttctccacgg aggggatgaa ctgtgtcggg atcaccccgc cgacgatctt gttcacgaag
     2281 tcgaagccgc cgccgcgttc gaggggttcg acctccacgt gcgcgatgcc gtactgcccg
     2341 tggccgccgc tctgcttcac gtgccggccg agggcctgca ccttctgccg gagggtctcc
     2401 ttgtacggga cccgggcggg gacggtcccg acgtcgacgc cgaacttccg ccgcatccgc
     2461 tccgtcatca cgtcgaggtg ggcttcgccg agcccggacg cgacggtctg gtgcgtctcg
     2521 gggttccgct ccacgaggaa ggtcgggtcc tcctccttga gccgggcgag ggcggtcgag
     2581 agcttctcct cgtccccctg cgtcttcggc tcgatcgcga cggagaacag cggctcgggc
     2641 cactccaccg cggggagcgt ggccgctccg tccttcgccg tcagggtgtc tccggtgtgc
     2701 gtgtggccga gcttggcgac ggccccgatg tcgcccgcga ccacctcggg cacgttctcg
     2761 tgctccttgc cccgcatcgt gaacagctgg gagacgcgct catcctcgtt ctgcgtggcg
     2821 ttgtggaccg tcgagtcggc gcggagccgc cccgagtaca cgcggaacag cgagatcctt
     2881 ccgacgtagg gatccgagat cgtcttgaag acgaaccccg tcagcgggcc gttcggatcc
     2941 ggggcccgct cctgctcctc gccgtcgcgg gtcatgacct tcacgggcgt gcggtcggtc
     3001 ggagcgggga acgcctctcg catgaactcg gcgagacggt cgacgccgac cgccttcgac
     3061 gcggagccgc agaggaccgg cgcgaacttc gcctgcacga gcccggcctt gacccccgtg
     3121 acgatctccc cagcggagag ctcgcccgtc tcgaggtact tctcgaggag cgtgtcgtcg
     3181 gcctcggcga ccgcctccgc gagcttctcg cggtagggat ccgccttccc cgcgatgtcg
     3241 tcgggccatt cgccctcggc gcccgcggga ccgtccgggt agcggtaggc cttccgggtg
     3301 agcaggtccg cgaccccctc gaggtcgtgc tcctccccga tcgggaactg gacgggggcg
     3361 acctgcgtgc cgaaggccgt gacgagctcg tcgagcgtcc gctggaacga ggcccgctcc
     3421 cggtcgagct tgttgatgaa gatcgcgcgt gggacccggg cctcggcagc gagctcccac
     3481 accatctcgt gctggacctc gacgccgtcg accgccgaga cgacgaacag gcacacgtcg
     3541 gcggcgcgga gcgcgacctt cacgtcgccg atgaagtcgg cgtatccggg ggcatcgagg
     3601 aggttgatct tcacgccctt ccactcgatc ggggcgaggg agagcgagac gctgatctgc
     3661 ttcttcgtct cctcgggctc gaagtcggag acggtgttgc cgtcctcgat ccggcccatc
     3721 cgggtgatcg cgccggcggt gtagagcaga gcctcggtca gggtcgtctt gccggacccc
     3781 ccgtggccga cgagcaggac gttgcggatc gccgtgggtt cgtaggtctt catcgtgctc
     3841 ctcggggtcg cgtacggcga ttatagtgag cggccgttcg gcccgagccc gccgcctgtt
     3901 aggctcggtg gccgcatgac ggagcgactc gacgtcccgt ccggccgccc ggcgttcccg
     3961 ctctccctcc tggtcgcggc cgtcgcgttc gtggccctgg gggcgtggtt cgccctcgat
     4021 ggtcgcgatc ccgccgccgg tgtcgccgtg ctcgtcgggc tcgcgctcct cgtcggcggg
     4081 gggcgctcgg cgcgcgcgat gggctcaccg ggcgtgatgt tcaccgactc ggcggtcgag
     4141 cggatctgcg acgcggccct gctcgcggcg atcgcgtggt cgaggctcga cgtcgcgccc
     4201 cgggtcgcga cggcggccct gatcgccctc gtgacctcct acctcgcgtc ctacctgcgc
     4261 gcgaagtcga ccgggctcgg gttcgcgcgc aggtaggacg cgaggtagga ggtcacgagg
     4321 g
//
LOCUS       DATLGG010000262         6136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_104002, whole genome shotgun sequence.
ACCESSION   DATLGG010000262 DATLGG010000000
VERSION     DATLGG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104002"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VM638_07520"
     CDS             <1..224
                     /locus_tag="VM638_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12307.1"
                     /translation="AEASAGAVTARAFAPLERAWPVARRILQPGGSFVYFAGGSMEDP
                     LAEARRVAGSEAQARLLESAPPLVIMTRR"
     gene            239..1114
                     /locus_tag="VM638_07525"
     CDS             239..1114
                     /locus_tag="VM638_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HVM12308.1"
                     /translation="MFPRPEVEGSPAAQARVLAVANQKGGVGKSTTAVNLSAALAEVG
                     RRVLVIDLDPQGNASTGLGIELERRRTTTYELLTTDGPAERAVLDTEVPGLWAIPSTI
                     DLAGAEIELVSQFSRELRLQRALQPLMGAYDFIVMDCPPSLGLLTVNALATAAEVIVP
                     IQCEYYALEGLGQLLRNVRLVQQSLNPGLKLSGIVLTMFDSRTKLSEQVVAEVRRFFG
                     NRVYDTVIPRTVRLSEAPGYGQPITVYDPSSTGARAYRSLAREVAERPVSDEPLPTEE
                     LPAVILPEPSPEAGS"
     gene            1111..1968
                     /locus_tag="VM638_07530"
     CDS             1111..1968
                     /locus_tag="VM638_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVM12309.1"
                     /translation="MTRQRGGLGRGLSALIPGAEEGGLLEIPVGAIVPNPRQPRERFD
                     ESALEGLAASIRDVGVLQPIAVRRLDDGGYELVAGERRLRAAKMAGLATIPAVVRETD
                     DADQLREALIENIQRENLNPLETAAAFQELLDDLQVSQEALAAKLGFSRAHIANTIRL
                     LQLPSDVQRLVAEGKLQAGHARALLGLDDDEARTALALRAVAEELSVRNVEDLVRAYA
                     AGGPEPAGRESQAPARDPRVAEVEEILADELGTKVRVQMGKRRGRIVVEFGSPDDLDR
                     IATRIVGGA"
     gene            complement(2085..3158)
                     /locus_tag="VM638_07535"
     CDS             complement(2085..3158)
                     /locus_tag="VM638_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HVM12310.1"
                     /translation="MRAIRAGESGEKVRDVQSRLIALGQTIDPSELEASHFGASTERA
                     VRAFQQERSLLVDAIVGPATWEELVEASYSLGDRLLYLRAPNLRGDDVRALQERLDIL
                     GFDPGRADGIFGEQTDRAVRDFQRNVGLEPDGIVGATTLEALRRLRPPGAGPGRTAVR
                     EGETLRSRGSLAGRTIALDAGHGPGDPGARGPAGLTEAEAALTLVEEVADQVRASGAT
                     PVLLRNPQDDPTTEERVDRARRSGADALVSIHLNSHRDGSAEGASCYYFGRLGNVSVP
                     GRALAEMVQDELTSRLGLQDGRTHPKAYPLLRETPMPAVQVEPCFITNPKEEAAIQEA
                     PFRQEVGRAVTAALARFFEGRAD"
     gene            complement(3202..3531)
                     /gene="trxA"
                     /locus_tag="VM638_07540"
     CDS             complement(3202..3531)
                     /gene="trxA"
                     /locus_tag="VM638_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVM12311.1"
                     /translation="MSENVMTATDAGFETEVLKSDQPVLVDFWADWCTPCHMVSPVVE
                     EIGREKAGVMKVAKLNVDDNPETARRFGVMSIPTLILFKDGEERARLIGARPKDDILR
                     GIDPHVA"
     gene            complement(3641..4576)
                     /gene="trxB"
                     /locus_tag="VM638_07545"
     CDS             complement(3641..4576)
                     /gene="trxB"
                     /locus_tag="VM638_07545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HVM12312.1"
                     /translation="MASVEPRKVVIIGSGPAGYTAALYAARANLRPLVLKGIESGGQL
                     MLTTEVENYPGFPEGVMGPELMDLMEKQAARFEAEMLHQAATRVDLSQRPFGVWSGDQ
                     EFRAQSVIVATGAKARMLGVPGEQELLGHGVSTCATCDGFFFRDRDLVVVGGGDSAME
                     EALFLSRFATKVTVVHRRKELRASKIMQDRAFANEKIDFLWDTVVDEVLGDGAVSGVR
                     LRNVVTGAVEDRPVAGMFVAIGHDPNTAIFRDQLETDADGYLVVKEPTTYTSVDGVFA
                     AGDAVDNIYRQAITAAGMGCKAAMDAERWLELQGM"
     gene            complement(4605..5441)
                     /locus_tag="VM638_07550"
     CDS             complement(4605..5441)
                     /locus_tag="VM638_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVM12313.1"
                     /translation="MSEHPHHLLAEYVDGTLHADELSHVERHLDGCPACRDETGIASS
                     AASALRDLPEAQPPLGTAERVLRHARRAPPPALAWRLAGAAAAAIVVAVAFVALRGPQ
                     DRAAVEPYAAERAGDAAAPADGSGGFGEAEGGETADATEERTALEASAASAYPSYRES
                     PRDYAPVQLVVLSRRVAREAREAMDAGLPSSAEAFYRTYDLRSLEPDAQRALSCVTQG
                     DPPDRAVVPFVIEAARFEGRPAYLAAFLLGPSPEAAFDRLQVVVVSRDGCTIRHFSQE
                     RL"
     gene            complement(5438..6004)
                     /locus_tag="VM638_07555"
     CDS             complement(5438..6004)
                     /locus_tag="VM638_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVM12314.1"
                     /translation="MNDPSDADLVERFLGGDRDAFATLVQRHTTKVYNVAYRMLGRPE
                     DARDAVQEVFLTCLRKLRGFEGRAAFTTWLYRVTVNVCHDALRRRAREQPAHEELPDL
                     PEDDRTEAAAAAADVHRALGAIPEEFRTVLVLHDLQGEPYERIAEVLGAPLGTVKSRL
                     HRGRVALGRALRGEQSGTPTPSNVEDRG"
BASE COUNT          823 a         2223 c         2212 g          878 t
ORIGIN      
        1 tcgcggaggc ctccgcgggg gccgtcaccg cccgcgcctt cgccccgctc gagcgcgcct
       61 ggccggtcgc gcggaggatc ctgcagcccg gcggctcgtt cgtgtacttc gccggcgggt
      121 cgatggagga ccccttggcg gaggcgcgcc gggtggcggg aagcgaagca caggctcgac
      181 tccttgaatc cgccccaccg ctcgttatca tgacccgtag gtgagcgcgg atccgtccat
      241 cttccctcga ccggaggtcg agggttctcc ggcagcgcag gcgcgcgtgc tcgcggtcgc
      301 caaccagaag ggtggcgtcg ggaagagcac gaccgcggtg aacctcagcg ccgcgctggc
      361 ggaggtcggc cgcagggtgc tcgtcatcga cctcgacccg caggggaacg cctccaccgg
      421 gctcggcatc gagctcgagc ggcgcaggac gacgacctac gagctgctca cgaccgacgg
      481 gcccgccgag cgtgcggtcc tcgatacgga ggtcccgggg ctctgggcga tcccgtcgac
      541 gatcgatctc gccggcgccg agatcgagct ggtcagccag ttctcgcgcg agctccggct
      601 gcagcgagcg ctccagccgc tgatgggcgc ctacgacttc atcgtgatgg actgcccgcc
      661 ctcgctcggg ctcctgaccg tcaacgcgct cgccacggcg gccgaggtca tcgtcccgat
      721 ccagtgcgag tactacgcgc tcgagggcct cggccagctc ctgcgcaacg tccgcctggt
      781 gcagcagagc ctcaacccgg ggttgaagct ctcggggatc gtgctgacga tgttcgacag
      841 ccgcacgaag ctgtcggagc aggtcgtggc ggaggtgcga cgcttcttcg gcaaccgggt
      901 gtatgacacc gtcatcccgc gaaccgtgcg cctctcggag gccccgggct acggccagcc
      961 catcaccgtc tacgatccgt cgtcgaccgg cgcccgggcc taccgctccc tcgcgcggga
     1021 ggtcgccgag cgtcccgtca gcgacgagcc gctccccacg gaggagctgc cggccgtcat
     1081 cctccccgag ccgtccccgg aggccgggtc gtgacgcgac agcgaggggg cctcggtcgt
     1141 ggcctctccg cgctcatccc cggcgccgag gagggcggac tcctcgagat ccccgtgggg
     1201 gcgatcgtgc ccaacccccg ccagccgcgc gagcgcttcg acgagtcggc gctcgagggg
     1261 ctcgcggcgt cgatccgcga cgtcggcgtg ctccagccga tcgcggtccg gcgcctcgac
     1321 gacgggggct acgagctcgt ggccggggag cggcgactgc gggcggcgaa gatggccggc
     1381 ctcgcgacga tccctgcggt cgtgcgtgag accgatgacg cggaccagct gcgcgaggcc
     1441 ctcatcgaga acatccagcg cgagaacctc aacccgctcg agacggcggc cgcgttccag
     1501 gagctcctcg acgacctcca ggtgtcccag gaagcgctcg cggcgaagct gggattctcc
     1561 cgggctcaca tcgcgaacac gatccgtctg ctccagctcc cgtccgacgt ccagcgactg
     1621 gtcgcggagg ggaagctaca ggcggggcac gcccgggccc tgctcgggct cgacgacgac
     1681 gaggcgcgca cggcgctcgc gctccgagcg gtcgccgagg agctctcggt gaggaacgtc
     1741 gaggacctcg tgcgtgccta cgcggcgggt ggacccgagc ccgcgggtcg ggagtcccag
     1801 gccccggcgc gcgacccgcg cgtggcggag gtcgaggaga tcctggcgga cgagctcggg
     1861 acgaaggtgc gggtccagat ggggaagcgg cgcggcagga tcgtcgttga gttcggctcc
     1921 ccggacgacc tcgatcggat cgcgaccagg atcgtcggcg gggcgtagca cggcctgcag
     1981 gggtaggatc gcgccgtgga agaagcacgc cgtgccgtca tctcgatctc gctcggcgtc
     2041 ctgctggggc tcgtcttgat cgggcgatcc cgccgcggtc gctcctagtc ggcccgaccc
     2101 tcgaagaagc gggcgagcgc agccgtgacg gcgcgcccga cctcctgccg gaagggcgcc
     2161 tcctggatcg ccgcctcctc cttcgggttc gtgatgaagc agggctcgac ctggaccgca
     2221 ggcatgggtg tctcgcgcag caacgggtac gccttcgggt gggtccgtcc gtcctgcagc
     2281 cccaagcggg aggtcagctc gtcctggacc atctcggcga gcgcccgacc ggggaccgag
     2341 acgttgccga gccgcccgaa gtagtagcag gacgctccct cggcggagcc gtcgcggtga
     2401 ctgttgaggt ggatcgacac gagcgcgtcg gcacccgacc ggcgggcccg gtcgacccgc
     2461 tcctcggtgg tggggtcgtc ctgggggttc cggagcagga ccggtgtcgc gccgctcgcc
     2521 cgcacctggt ccgcgacctc ctcgacgagg gtgagcgccg cctccgcctc ggtcagcccc
     2581 gcggggccgc gggcgccggg atcccccggg ccgtggcccg cgtcgagggc gatggtgcgt
     2641 ccggcgagcg acccacggga gcggagggtc tctccctccc ggacggcggt gcggccgggg
     2701 ccggcgcccg gggggcgcag ccggcgaagg gcctcgagcg tcgtggcccc gacgatgccg
     2761 tccggctcga ggcccacgtt ccgctggaag tcgcgcacgg cgcggtcggt ctgctctccg
     2821 aagatgccgt cggctcgacc cgggtcgaag ccgaggatgt ccagccgctc ctgcagcgcg
     2881 cgaacgtcgt ctccgcgcag gttcggagcc cgcaggtaga gcagtcggtc gccgagggag
     2941 tagctcgcct cgaccagctc ctcccaggtg gcgggaccga cgatcgcatc cacgagcagc
     3001 gatcgttcct gctggaaggc gcggaccgcg cgctccgtcg aagcgccgaa gtggctggct
     3061 tcgagctcgc tggggtcgat cgtctgcccg agggcgatca ggcgcgactg cacgtcgcgg
     3121 accttctcgc cggactcgcc ggcccggatc gccctcatcg agcgggtctc ccccctgcgc
     3181 gggggctccg gcaccgccgg atcaggccac gtgggggtcg atgccccgca ggatgtcgtc
     3241 cttcggccgg gccccgatca gtcgcgcccg ctcctcgccg tccttgaaca ggatcaacgt
     3301 cgggatgctc atgacgccga agcgccgcgc cgtctcgggg ttgtcgtcga cgttcagctt
     3361 cgcgaccttc atcacgccgg ccttctcgcg gccgatctcc tcgacgaccg ggctcaccat
     3421 gtggcacggg gtgcaccagt ccgcccagaa gtccaccagc accggctgat ccgacttcag
     3481 gacctcggtc tcgaatcccg catcggtcgc ggtcatcacg ttctcgctca tgcgtcctcc
     3541 cagctccggt cgtcgctcac aacgggccta gaccccagga tattccctcg cgtccgcagc
     3601 gtctcacctg cggcgttccg cgccggggcc gcgcggctgg ctacatgccc tggagctcga
     3661 gccagcgctc cgcgtccatc gcggccttgc agcccatccc cgcggcggtg atcgcctggc
     3721 ggtagatgtt gtccacggcg tcacccgcgg cgaacacgcc atcgacgctc gtgtacgtcg
     3781 tcggctcctt caccacgagg tagccgtcgg cgtcggtctc gagctggtca cggaagatcg
     3841 ccgtgttcgg gtcgtgtccg atggcgacga acatcccggc gaccggccgg tcctcgacgg
     3901 cgcccgtcac gacgttgcgc aggcgcaccc cgctcacggc tccatccccg aggacctcgt
     3961 cgacgaccgt gtcccacagg aagtcgatct tctcgttcgc gaaggcgcgg tcctgcatga
     4021 tcttcgatgc gcgcagctcc ttgcggcgat gcacgacggt caccttcgtg gcgaaccgcg
     4081 agaggaacag cgcctcctcc atcgccgagt caccaccgcc gaccaccacg aggtcccggt
     4141 cgcggaagaa gaacccgtcg caggtcgcgc aggtcgagac gccgtgcccg aggagctcct
     4201 gctcgccggg gaccccgagc atccgggcct tcgcgcccgt tgcgacgatc accgactgcg
     4261 cccggaactc ctggtcgccg gaccagacgc cgaagggccg ctgcgagaga tccaccctcg
     4321 tcgcggcctg atggagcatc tcggcctcga agcgggcggc ctgcttctcc atgaggtcca
     4381 tgagctcggg gcccatcacc ccttcgggga acccggggta gttctcgacc tccgtcgtga
     4441 gcatcagctg cccgccggat tcgatcccct tgaggacgag cgggcggagg ttcgcgcggg
     4501 cggcgtagag ggccgccgtg tagcccgccg gccccgatcc gatgatgacg accttgcgcg
     4561 gctccacgga tgccattctg ccacagggcg tctcggaccc ccgtctagag ccgctcctgg
     4621 gagaagtgcc ggatcgtgca gccgtcgcgt gagaccacga cgacctggag gcggtcgaag
     4681 gcggcctcgg gggaggggcc gagcaggaag gccgcgaggt aggcggggcg gccttcgaac
     4741 ctcgccgcct cgatcacgaa cggaacgacc gcccggtccg gcggatcacc ctgggtaacg
     4801 caggagaggg cgcgctgcgc gtccggctcg agcgagcgga ggtcgtaggt gcggtagaac
     4861 gcttccgcgg acgacggcaa gccggcgtcc atcgcctccc gggcctcccg cgcgacccgg
     4921 cgggagagga cgacgagctg gacgggggcg tagtccctcg gggactcgcg gtacgagggg
     4981 tacgcgctcg cggcgctcgc ctcgagggcg gttcgctcct ccgtcgcatc agcggtctct
     5041 ccgccctcgg cctcgccgaa gcctccgctg ccgtcggccg gggcggccgc gtcgcccgcg
     5101 cgttccgccg cgtagggctc cacggcggcc cggtcctgcg gcccccggag cgcgacgaag
     5161 gcaacggcca cgacgatcgc ggccgcggca gcgcccgcga gacgccaggc cagggcgggc
     5221 ggcggcgcac ggcgggcgtg ccgcaggacc cgttcggccg tcccgagggg cggctgggcc
     5281 tcgggcaggt ctcggagcgc ggaggccgcg ctggatgcga tccccgtctc gtcccggcat
     5341 gccggacacc cgtcgaggtg acgctcgacg tgcgagagct cgtcggcgtg gagggtaccg
     5401 tcgacgtact cggccaggag gtggtggggg tgctcgctca tcctcgatcc tcgacgttgg
     5461 acggcgtggg cgtccccgat tgttccccac ggagcgcccg gccgagcgcg acccggccgc
     5521 ggtgcagccg ggacttcacg gtgccgagcg gtgccccgag cacctccgcg atccgctcgt
     5581 agggctcacc ctggaggtca tggaggacga ggaccgttcg gaactcctcg gggatcgccc
     5641 ccagcgcgcg gtggacgtcg gcggccgcgg ccgccgcctc ggtccggtcg tcctccggca
     5701 ggtcggggag ctcttcgtgg gccggctgct cgcgcgcgcg tcgccggagc gcgtcgtggc
     5761 acacgttcac cgtcacccgg tagagccagg tcgtgaacgc ggcgcgtccc tcgaaccccc
     5821 ggagcttccg caggcaggtc aggaagacct cctggacggc gtcacgcgca tcctccggtc
     5881 ggccgagcat ccggtaggcc acgttgtaga ccttcgtggt gtggcgctgc acgagcgtgg
     5941 cgaaggcgtc gcggtcgccg ccgaggaacc gttcgacgag atcggcgtcc gatggatcgt
     6001 tcatccgagc acctcgacct cggcgatcgc ggcgcggtag ccgtcatccg cctgggcgag
     6061 ctccgtcacc cacacgagga ggtaccggta ctcggccgga cgcagcgcga gctcggtgcg
     6121 ccggcgggcc gtgaaa
//
LOCUS       DATLGG010000263         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_63125, whole genome shotgun sequence.
ACCESSION   DATLGG010000263 DATLGG010000000
VERSION     DATLGG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63125"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VM638_07560"
     CDS             <1..347
                     /locus_tag="VM638_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVM12315.1"
                     /translation="AAGPRHAADLARRGRVLNRLISPVRKDTLFVSALAVAPTHRGRG
                     YGTALLERIIAGAQHLGLGVALDAAMEDEVGRKLYARLGFREVDARPTTDVDRELIPV
                     DGLVRMERPAGA"
     gene            complement(370..1854)
                     /locus_tag="VM638_07565"
     CDS             complement(370..1854)
                     /locus_tag="VM638_07565"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVM12316.1"
                     /translation="MLAASFRRSWFVATGAALTLVLAALAAPPATAELSGDPVAGSLQ
                     QVYYAGQAAGGGSIPSTPIPDCKDDETDPPDCWRGQKLTLQAARIGQTAAEPTIGVAK
                     DGTAYMAGSTLVVNTDVVWGVAKTDARRSTDGGKTWTSIHPKVPVAGENLPPGNADPM
                     IWVDTDTGRVFTFDLLSACNWLSFSDDKGESWTNVPIACGNVPVDHQTIVSAKPRNGL
                     STVGYPNVLYWCANRVVDNACGRSLDGGLTWTPTIGFPFNLQTSCRGLSGHLEADPDG
                     RIYLPTSQTCGTPWIARSEDNGDTWTRHQVSTLTSATPHTSVAADADGNLYYVWIRNQ
                     SGNPTPYLAVSKNRGQTWGAPMMIAPPGVIQANFPVVAAGDAGRIAITFPSTRSTAAN
                     RAWDQTVIVSTNALDERPVFLSATGNPPTDPIHRGSCGPGRCAGLWDFLDIQVSPAGE
                     AWAAASDDCVGACNNANAATSATKVGDGIAIRQIGGPLLRVPQG"
     gene            complement(1943..2779)
                     /locus_tag="VM638_07570"
     CDS             complement(1943..2779)
                     /locus_tag="VM638_07570"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HVM12317.1"
                     /translation="MTLDRKWLLGVARGEREALGRTIQYTPPEYWESTSPAEAWERLR
                     DILAHLAANEVSAAALVGDEEATELEEYGKSLPKGEPFSAEGFNRWAVERRADQGHLT
                     LALDWGRAADLLLSRASQLSDDDWNAKVVTWLAGDIKVGYLVQSRVAEWWIHGEDFRT
                     GGRLPPRVQHPPIFVTNDLAVRMIPYALEVAGVSFPGRTVRIDLTAAGGGEWHQGLER
                     GHVPEDGRPPDAWITGRADAFAMVAANRVDLEVCLYDGILNVGGDLEVAQTVLRTLRV
                     FA"
     gene            2826..>3520
                     /locus_tag="VM638_07575"
     CDS             2826..>3520
                     /locus_tag="VM638_07575"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HVM12318.1"
                     /translation="MRILVAPDKFKGTLAGEEVADAIARGWSRRRPRDAIERVPVADG
                     GDGTLEALVSALGGVWRSARVSGPLGDEVEAEYAVAETSGRRVAVVEMARASGLALVA
                     DRQDPLGASTRGTGELIREALRERPDVLLVCIGGSATTDGGAGMAQALGVRLVDDRGD
                     DLPPGGAALERLAKIDPSGLEPALAGVEIVVASDVDNPLTGPQGAAAVYGLQKGCGPE
                     EIRRLDAALERFQ"
BASE COUNT          465 a         1206 c         1333 g          516 t
ORIGIN      
        1 gggcagcggg cccgcgacac gccgcggacc tcgcccgtcg cggacgggtg ctgaaccggc
       61 tgatctcccc cgtccggaag gacacgctgt tcgtgagcgc cctcgcggtc gccccgacgc
      121 accggggacg gggatacgga acggcgctcc tggagcgcat catcgccggc gctcagcacc
      181 tcggcctggg cgtcgccctc gacgcggcga tggaggacga ggtcggccgc aagctgtacg
      241 cacggctggg cttccgtgag gtggacgcgc gcccgaccac ggacgtggac cgggagctga
      301 tcccggtcga cggcctcgtt cggatggagc gtccggccgg cgcctgagcg acgccggccg
      361 gcacacgact cagccctgcg gaacgcgcag cagcggaccg ccgatctgac ggatcgcgat
      421 cccgtcgccg accttcgtcg cggaggtggc cgcgttcgcg ttgttgcagg cacccacgca
      481 gtcatccgag gccgccgccc acgcctcgcc cgcgggcgag acctggatgt cgaggaagtc
      541 ccacaggccg gcgcaccgcc cgggaccgca gcttccgcgg tggatcggat cggtcggcgg
      601 gttccccgtc gcggatagga agacggggcg ctcgtcgagc gcgttcgtcg agacgatcac
      661 ggtctggtcc cacgcccggt tcgcggccgt gctccgggtc gaggggaagg tgatcgcgat
      721 cctgcccgcg tcgccggccg cgaccaccgg gaagttcgcc tggatcacgc ccggcggcgc
      781 gatcatcatc ggcgctcccc aggtctggcc gcggttcttc gagacggcca ggtacggggt
      841 ggggttcccg gactggttgc ggatccagac gtagtagagg ttcccgtcgg cgtcggcggc
      901 caccgacgtg tgcggcgtcg cgctcgtgag cgtgctcacc tggtggcgcg tccacgtgtc
      961 gccgttgtcc tcgctccgcg cgatccacgg ggtgccgcag gtctggctcg tgggcaggta
     1021 gatccgcccg tccggatcgg cctcgaggtg gccgctgagc ccccggcagc tcgtctgcag
     1081 gttgaacggg aagccgatcg tcggggtcca cgtgaggccg ccgtccaggc tgcggccgca
     1141 agcgttgtcg acgacgcggt tggcgcacca gtacaggacg ttcgggtagc ccacggtgga
     1201 cagtccgttc cgcggcttcg ccgacacgat cgtctggtgg tcgaccggaa cgttgccgca
     1261 ggcgatcggg acgttggtcc agctctcgcc cttgtcgtcg gagaagctca gccagttgca
     1321 ggcgctcagc agatcgaacg tgaagacccg gccggtgtcg gtgtcgaccc agatcatcgg
     1381 gtcggcgttg ccgggtggaa ggttctcccc ggcaacgggg accttcggat ggatcgaggt
     1441 ccaggtcttc ccaccgtcgg tcgaccggcg agcatcggtc ttcgcgaccc cccacacgac
     1501 gtcggtgttg acgaccaagg tggacccggc catgtaggcc gtgccgtcct tggcgacgcc
     1561 gatcgtcggc tcggctgccg tctggccgat ccgtgcggcc tgcagggtca gcttctggcc
     1621 acgccagcag tcgggcgggt ccgtctcgtc gtccttgcag tccgggatcg gggtgctcgg
     1681 gatcgaccct ccgccggctg cttgcccggc gtagtacacc tgctggaggg agcccgcgac
     1741 cggatcgccg gagagctcgg cggtggcggg aggagcggcg agcgcagcga ggaccagggt
     1801 cagggccgcg ccggtcgcga cgaaccacga cctgcggaag gatgcggcga gcatgtccgt
     1861 cctttctgtc ggtgaacgtt ccctggttgg ttcgacggag gacgccccgg ctcctgccga
     1921 tcagaggcac tttctggacg ggttaggcga agacgcggag cgtgcgcagg acggtctgtg
     1981 ccacctcgag gtctccgccg acgttcagga tcccgtcgta gaggcagacc tcgagatcca
     2041 cgcggttcgc cgcgaccatc gcgaaggcgt cggcgcgccc ggtgatccag gcgtcaggcg
     2101 ggcgtccgtc ctccggcacg tgtccccgct cgagaccctg gtgccactcc ccgccaccgg
     2161 cggcggtgag gtcgatccgg accgtccggc cggggaagga gacgccggcg acctcgagcg
     2221 cgtacgggat catgcggacg gcgaggtcgt tcgtgacgaa gatgggcggg tgttggacgc
     2281 gcggcgggag acggcccccg gtgcggaagt cctccccgtg gatccaccat tccgcgacgc
     2341 gcgactgcac gaggtagccg accttgatgt ccccagcgag ccaggtgacg accttcgcgt
     2401 tccaatcgtc gtcgctcagc tgagaggcgc gggacagcag caggtccgcg gcgcgccccc
     2461 agtcgagggc gagggtgagg tggccctggt cggcccgccg ttcgaccgcc cagcggttga
     2521 acccctcggc cgagaacggc tcgcccttgg ggagggactt cccgtactcc tcgagctcgg
     2581 tggcctcctc gtccccgacg agcgcggctg ccgagacctc gttcgcggcc aggtgcgcca
     2641 ggatgtcgcg gagacgctcc cacgcctccg cgggggaggt cgactcccag tactccggcg
     2701 gcgtgtactg gatcgtccgc ccgagcgcct cgcgttcccc gcgcgcgacg cccagcagcc
     2761 acttccggtc cagggtcatg tctcgcggca tcataccggt cggtcgcgcc ggtagggtgg
     2821 tcgtgatgcg gatcctcgtc gcgccggaca agttcaaggg gacgctcgcg ggcgaggagg
     2881 tcgcggacgc gatcgcccgg gggtggtcgc gccggcgacc gcgggacgcg atcgagcggg
     2941 tccccgtggc cgacggcggc gacggcacgc tggaggcgct cgtgtcagcc ctcggaggtg
     3001 tctggcgcag cgcccgcgtg agcgggccgc tcggcgacga ggtcgaggcc gagtatgcgg
     3061 tcgcggagac gagcggccgg cgggtcgccg tcgtggagat ggctcgcgca tccgggctcg
     3121 cgctcgtggc ggatcggcag gacccccttg gcgccagcac ccgcggaacg ggcgagctga
     3181 tccgcgaggc gctgcgcgag cgacccgacg tgctcctcgt ctgcatcggg ggcagcgcga
     3241 cgaccgacgg gggagcgggc atggcgcagg cgctcggcgt gcgcctggtc gatgaccggg
     3301 gcgacgacct ccccccgggt ggagcggcgc tcgagcggct ggcgaagatc gacccgtccg
     3361 gcctcgagcc ggccctcgcc ggtgtcgaga tcgtcgtcgc gtccgacgtg gacaacccgc
     3421 tgaccggccc gcagggggcc gccgccgtct acgggctgca gaaggggtgc gggccggagg
     3481 agatcaggag gctggacgcg gcgctggagc gcttccagga
//
LOCUS       DATLGG010000264         5448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_76037, whole genome shotgun sequence.
ACCESSION   DATLGG010000264 DATLGG010000000
VERSION     DATLGG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76037"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VM638_07580"
     CDS             complement(<1..444)
                     /locus_tag="VM638_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HVM12319.1"
                     /translation="MFEDLIRGWDGEETLVHYDAPTDTWMFICIHSTLLGPAAGGCRM
                     KTYESTDDALRDCMRLAGGMTLKNAVGGLPMGGGKSVIAVSEVPPPGSERRAEIVRRF
                     GMMVGSLKGSYWSGPDMNTSSADMDEIYRVAPYTFGRSPALGGSGD"
     gene            594..800
                     /locus_tag="VM638_07585"
     CDS             594..800
                     /locus_tag="VM638_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HVM12320.1"
                     /translation="MTTNVQVYPNPDDTQWGVRVVGDDETEPQLFSSREEAEEVARRL
                     AEERGGRLVLHDEAGMTEKKEDAG"
     gene            797..1126
                     /locus_tag="VM638_07590"
     CDS             797..1126
                     /locus_tag="VM638_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12321.1"
                     /translation="MSWTGSSTERAFDAPKDRVFQAALNAAAKVRMKVKEADPEAGRL
                     LLRSGMSMTSWGENLVLQLTETDGRTTVSLDSKLLVSTNIFAKGKHQRNFDRLLGAIA
                     EDLGVPT"
     gene            1171..1623
                     /locus_tag="VM638_07595"
     CDS             1171..1623
                     /locus_tag="VM638_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_6728 family protein"
                     /protein_id="HVM12322.1"
                     /translation="MQTFLPYPDFAETARVLDRARLGKQRVEALQILRAIHIPTYGWQ
                     HHPAVRMWRGYEDALVCYGVTICDEWIAQGRPDTVRPQILEHAHRGTARTQDELRAAG
                     ALPPWLGDEAFHRAHRSALLRKDPEHYREIFGDVPDDLPYVWPVPKGG"
     gene            complement(1593..2381)
                     /locus_tag="VM638_07600"
     CDS             complement(1593..2381)
                     /locus_tag="VM638_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HVM12323.1"
                     /translation="MPEGHLIHHLAGTHARDLAGAPTAVSSPQGRFAREAATLDGRSL
                     DEVEAFGKHLFYRWGNDVVHVHLGKQGLFLAFPTPAPPPRGGVRLRIAGDRATIDLIA
                     PRICELGTETTRDAVVSRLGPDPLRPETRRDDVLEALPRSARPIGAVLLDQSVVSGVG
                     NVLRAEALSIARIHPSTPANLLAPGDLARLWDELVALMRRSEELGRIRTIITDPEVPD
                     VETRFVYKQERCRRCGTPVEVEEVGGRTSYACPRCQPPFGTGQT"
     gene            complement(2407..3285)
                     /gene="ligD"
                     /locus_tag="VM638_07605"
     CDS             complement(2407..3285)
                     /gene="ligD"
                     /locus_tag="VM638_07605"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVM12324.1"
                     /translation="MASVPEVRRTNPDKLMWPKDRVTKHDLISYYEAVSNLLVEELRG
                     RPLTVVRAPDGIDGFRFFQKQAPDSTPPWVETVLLPAPASRRKEVRFLLCNRPETLVW
                     LGNQAALELHPSLASVESPRHPDALVFDLDPPERGFAKAIAAASAFREVLDDLNLRSV
                     VKTSGGKGLHIFVPIEGATFEQTHAAAEGIGRLAIERSSIPMTFAFLKKERRGRLLID
                     IGRNGPGATTVATFSPRARRGAPVSFPVLWEDIDRIDPRDFTIRTAPDLLSTPAVKAW
                     MKERRRTQTLPEIPVG"
     gene            complement(3359..3432)
                     /locus_tag="VM638_07610"
     tRNA            complement(3359..3432)
                     /locus_tag="VM638_07610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3396..3398),aa:Thr,seq:cgt)
     gene            3685..4302
                     /locus_tag="VM638_07615"
     CDS             3685..4302
                     /locus_tag="VM638_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12325.1"
                     /translation="MRTKKLLVPLAAATLLLSGCVWQLAGVSYSAYTIDEGDSTKAVL
                     RVRPSDTSAQRMYVFVMTGVANVGSDSNTDNDYLGIGKHTWDTGGAYNGPKAMVEDTG
                     LRDFVLANDLCSTDGFNASDLSGLAWELHRTTGQINDAGRTALAKISASLRARSSAGG
                     SMTHQVVHAVGVWIDNENSGQGDGSFTNDSPMCTSGSFTFLRVRP"
     gene            4408..>5448
                     /locus_tag="VM638_07620"
     CDS             4408..>5448
                     /locus_tag="VM638_07620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12326.1"
                     /translation="MTPADQASPSSTFDIVCFSHLRWRFVFQRPQHLLSRWAREHRVF
                     YVEEPVEHDGPTFLEITEVQERVWVVVPFLPRGTTPEEAERTQRGLVEHLIRDRKIGR
                     YVLWFYSPAAVPLARNLEPLATVYDCMDELSAFEEAARDVAEREGELFDSADLVFTGG
                     QSLFEAKRERHRHVHLFPSSVDVPHFASARTQARDPEDQAGIGRPRLGFFGVIDERLD
                     RDLLAGIARLRPDWQIVMVGPVVKIPPEALPTAPNIHYLGAKPYEELPAYVGGWDLAI
                     LPFARNAATRFISPTKTPEYLAAGRPVVSTSITDVVRPYRDLGLVRIADDPEGFVAAA
                     ETAMAEDEGERLG"
BASE COUNT          880 a         1856 c         1901 g          811 t
ORIGIN      
        1 gtctcccgag ccgccgagcg cgggtgagcg gccgaacgtg tagggagcca cccggtagat
       61 ctcgtccatg tcggccgacg aggtgttcat gtccgggccg gaccagtagc tgcccttgag
      121 cgagccgacc atcatcccga accggcgaac gatctcggcg cgtcgctcgc ttcccggggg
      181 cggaacctcc gagacggcga tcacgctctt gcccccgccc atgggcagac cgccgacggc
      241 gttcttcagc gtcatcccgc ccgcgaggcg catgcaatcc cgcagggcgt cgtccgtgga
      301 ctcgtaggtc ttcatgcgac atccgcctgc cgccgggccc aggagggtcg agtggatgca
      361 gatgaacatc cacgtgtcgg tgggggcgtc gtagtggacg agcgtctcct cgccgtccca
      421 cccgcggatc agatcctcga acacccctca cctcccgtcg gtcgcgtcga gatagtagcc
      481 gcgggccgga ggcctcgtct gctcgccaca caccgcccgc aaccgagggt cttcgcccgg
      541 acgtgttcat cctcggcgcg agagggaaag caaggtccga ggaggtgacg tcgatgacga
      601 cgaacgtgca ggtctacccg aacccggacg acacgcagtg gggcgtcagg gtcgtgggcg
      661 acgacgagac cgagccgcaa ctgttctcca gccgggagga ggccgaagag gtcgcgaggc
      721 gcctcgccga ggagcgtggc ggccgcctcg tcctccacga cgaagccggc atgaccgaga
      781 agaaggaaga cgctggttga gctggaccgg ctcctcgacg gagcgggcgt tcgacgcgcc
      841 gaaggaccga gtcttccagg cagcgctgaa cgccgcggcg aaggtgagga tgaaggtgaa
      901 ggaggccgac cccgaggcgg ggcgcctcct gctccgaagc gggatgtcga tgacatcgtg
      961 gggcgagaac ctggtccttc agctcaccga gaccgacggc aggacgacgg tctccctcga
     1021 ctccaagctc ctcgtgtcga cgaacatctt cgccaagggc aagcaccaga ggaacttcga
     1081 tcgcctgctc ggtgcgatcg cggaggacct gggcgtaccg acctgatccc aggtcgccgc
     1141 ggtgagcggt ccgtggggta ggaaccgggc gtgcagacgt tcttgcccta ccccgacttc
     1201 gccgagacgg cgcgggtgct cgaccgcgcg aggctcggga agcagcgcgt ggaggctctg
     1261 cagatcctcc gcgcgatcca catccccacg tacgggtggc agcaccaccc cgcggtgcgc
     1321 atgtggcgcg gctacgagga cgcgctcgtg tgctacggcg tgacgatctg tgacgagtgg
     1381 atcgcgcagg gacgccccga caccgtgcgg ccccagatcc tcgagcacgc ccaccgcggg
     1441 acggcacgga cccaggacga gctccgggcc gccggcgcgc tgccgccgtg gctgggcgac
     1501 gaggcgttcc accgagccca ccggtcggcc ctgctgcgga aggatccgga gcactaccgg
     1561 gagatcttcg gggacgtgcc ggatgacctg ccctacgtct ggccggtgcc gaagggcggc
     1621 tgacaccggg gacaggcgta cgaggtccgt ccgccgacct cctccacctc gaccggcgtg
     1681 ccgcaccgac ggcaacgctc ctgcttgtag acgaaccgcg tctcgacatc gggcacctcg
     1741 ggatcggtga tgatcgtcct gatgcgcccg agctcctcgg atcggcgcat caacgccacc
     1801 agctcgtccc agagcctcgc gagatcgccc ggtgcgagaa ggttcgcggg ggtgctcgga
     1861 tggatgcgcg cgatggagag ggcctcagcg cgcagcacgt tgcccacacc cgacacgacg
     1921 gattggtcga gcagcaccgc gccgatgggc cgagcggatc gggggagcgc ctccagcacg
     1981 tcatcgcggc gcgtctccgg acggagcgga tcgggcccga gccgggagac gaccgcgtcg
     2041 cgggtcgtct cggttccgag ctcgcagatg cgtggcgcga tgaggtcgat cgttgcgcgg
     2101 tcgcccgcga tgcgcagccg aacgccgcct cggggcggtg gcgccggcgt tgggaacgcc
     2161 aggaacagcc cctgcttccc gaggtgcacg tgcacgacgt cgttccccca gcgatagaag
     2221 aggtgcttgc cgaaggcctc gacctcgtcc agcgagcgtc cgtcgagcgt cgcggcttcg
     2281 cgagcgaacc gtccctgcgg cgacgacacc gcggtgggcg cgccggccag atcgcgggcg
     2341 tgcgttccag ccaggtggtg gatcaggtga ccttcgggca ccggggcccc tccagcgtcg
     2401 gagcggtcat ccgacgggga tctcggggag cgtctgtgtc cgccgtcgtt ccttcatcca
     2461 cgccttcacg gcgggggtgg agagcagatc cggagccgtg cggatcgtga agtcccgcgg
     2521 gtcgatccgg tcgatgtcct cccagagcac cgggaaggag acgggcgcgc cgcgccgcgc
     2581 gcgcggtgag aacgtcgcca cggtcgtcgc cccggggccg ttgcggccga tgtcgatcag
     2641 gagtcgcccg cgacgctcct tcttcaggaa cgcgaaggtc atcgggatcg aggatcgctc
     2701 gatcgcgagg cgcccgatcc cttcggcggc cgcatgcgtc tgctcgaacg tcgcgccctc
     2761 gatgggaacg aagatgtgga gccccttgcc gcccgaggtc ttcaccacgg agcgaaggtt
     2821 caggtcatcc aggacctctc ggaacgcgct cgcagcggcg atggccttcg cgaatccgcg
     2881 ctccggcggg tcgaggtcga acacgagggc gtcgggatgc cgggggctct cgacggaggc
     2941 gagcgacgga tgcagctcga gcgccgcctg gttcccgagc cacaccagcg tctcgggtcg
     3001 gttgcacagg aggaagcgaa cctccttgcg gcgggaagcc ggcgcgggca gcagcaccgt
     3061 ctcgacccac gggggggtgg agtcaggagc ctgcttctgg aagaaccgga agccgtcgat
     3121 gccgtcgggg gcgcggacca cggtgagcgg cctcccgcgc agctcctcga cgaggaggtt
     3181 cgacaccgct tcgtagtacg agatcaggtc gtgcttcgtg acccggtcct tgggccacat
     3241 gagcttgtcc ggattcgtcc gccggacctc ggggacgctc gccatcgtcg acaccgtagg
     3301 tgcctacccc gtcagcaagc gtggcaagcc ggcggcacga tgacctgcgc cgatacagag
     3361 ccggaggtgg gattcgaacc cacgacccca cgattacgaa tcgtgtgctc tgccgaactg
     3421 agctactccg gcggctggcc agatgatagc gcggggggca gcccacggga ggtcgccgat
     3481 gcgaccgtcg atccccccgt atcccctgaa aaccccttga cggacccgtc ccgcgaagcc
     3541 atgattcgcc cgggcttgcc tcgattcgcc cgcaagcgct tgctcaacgg agggagggaa
     3601 ggagggaacc agatctcgcg cgcctagaag gctgcccagg acccgtacgt ccaacgcgat
     3661 cttcgatccg aagggagtca tgtgatgaga acgaagaagc tcctcgtgcc gctcgccgcg
     3721 gcgacgctcc tgctgtccgg ctgcgtctgg cagctcgccg gggtcagcta cagcgcctac
     3781 acgatcgatg aaggcgacag caccaaggct gtgctgaggg ttcggccgtc ggacacgagc
     3841 gcgcagcgga tgtatgtctt cgtcatgacc ggcgtcgcca acgtcgggag cgactcgaac
     3901 accgacaacg actacctcgg catcggcaag cacacgtggg acaccggagg cgcgtacaac
     3961 ggaccgaagg cgatggtcga ggacacgggt ctgcgggact tcgtcctcgc gaacgatctg
     4021 tgctccacgg acggcttcaa cgcctcggac ctgtccgggt tggcctggga gcttcaccgg
     4081 acgacgggtc agatcaacga tgccggtcgc accgcgctgg ccaagatctc ggcctccctt
     4141 cgcgcccgca gcagcgccgg tggcagcatg acgcaccagg tggtgcacgc cgtcggcgtc
     4201 tggatcgaca acgagaacag cggacagggt gacggatcct tcacgaacga ctccccgatg
     4261 tgcaccagcg ggtcgttcac gttcctccgc gtcaggccgt agttccggag accacgcggt
     4321 tcactgagcg gccggccttc gggccggccg ctcccctttg accgggtcgt caggttaggc
     4381 tcgttctggt ctgggtacaa cccggcagtg acccctgctg accaagcgtc cccttcatcg
     4441 accttcgaca tcgtctgctt ctcgcatctg cggtggcggt tcgtgttcca gcgtccgcag
     4501 cacctactga gtaggtgggc gcgggagcat cgcgtcttct acgtcgagga gccggtcgag
     4561 cacgacgggc cgaccttcct cgagatcacc gaggtccagg aacgcgtgtg ggtggtcgtc
     4621 ccgttccttc cccgcgggac gacccccgag gaggcggagc ggacgcagcg cgggctcgtc
     4681 gagcacctca tccgggaccg gaagatcggt cgctacgtcc tgtggttcta ctcgcccgcc
     4741 gcggtgccgc tcgcgcggaa cctcgagccg ctggcgaccg tgtacgactg catggacgag
     4801 ctctccgcgt tcgaggaagc ggcgcgggac gtggcggagc gtgagggtga gctcttcgac
     4861 agcgccgacc tcgtgttcac cggtggccag agcctgttcg aggccaagcg cgaacggcac
     4921 cggcacgtgc acctgttccc gagcagcgtc gatgtccctc acttcgcgtc ggcgcgcacg
     4981 caggcccgcg accccgagga tcaagccggc atcgggcggc cacgtctcgg cttcttcggt
     5041 gtgatcgacg agcgcctcga ccgggacctc ctcgccggga tcgcgcggct gcggcccgat
     5101 tggcagatcg tgatggtcgg cccggtcgtg aagatcccac cggaggcgct gccgacggcc
     5161 cccaacatcc actacctcgg cgcgaagccg tacgaggagc ttcccgcgta cgtgggcggg
     5221 tgggacctcg cgatcctgcc gttcgcgcgc aacgcggcca cccggttcat cagtccgacg
     5281 aagacccccg agtacctcgc cgcggggcgg ccggtcgtct ccacctcgat caccgacgtc
     5341 gtccggccgt accgggacct cgggctcgtc cgcatcgccg acgatcccga aggcttcgtc
     5401 gcggcggccg aaaccgcgat ggccgaggac gaaggcgagc ggctgggc
//
LOCUS       DATLGG010000265         7925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_172731, whole genome shotgun sequence.
ACCESSION   DATLGG010000265 DATLGG010000000
VERSION     DATLGG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172731"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VM638_07625"
     CDS             complement(<1..1458)
                     /locus_tag="VM638_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12327.1"
                     /translation="MSRKLIPFALLALLATACGEGGVAVQEGEVPANAFVVQVADAGP
                     GIGHAGVSLTTDAEGNPHLAYIALEPEAEEGEPAAAPDPGGPTPPAVLHAHVVDGVWT
                     HSPVAEETGVTAEDTVAIAVGEDGTHHVAWTEGGAGVRYSSNAGGSFSEPAVVHDGTV
                     SGVSIALEGETPLVTFYEEPAQGETQTVRLATSDGSAWSVETVAEASPPEGGTTAVGS
                     GAQGTLVAYGSEGRTLLTRRNGEVWESEGVDDGGIGVSMSLDGDGNPHLAYRDAQGAV
                     RHSHSIGGGDWETTDVGEAATGGTGIAVDGEGIHHVAWSDDQGVVYASNEQGSFAADR
                     FPQFAGGGSPSVASGGEGAVHLAWGDPQMSAVKLGTRGGDEPFLAAPPAGPEPTGEPT
                     EEPTTAPACQPEGTELQIVAENIAFDKDCLAAPVGEGVTIEFENLDTALHNVAIYTDD
                     TLSEPIFREDPFAGPQTVTYDVPAVDEAGSYYFHCD"
     gene            complement(1501..4356)
                     /gene="uvrA"
                     /locus_tag="VM638_07630"
     CDS             complement(1501..4356)
                     /gene="uvrA"
                     /locus_tag="VM638_07630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HVM12328.1"
                     /translation="MGTDRIVIHGAREHNLKDVHLELPRDRLIVFTGLSGSGKSSLAF
                     DTVYAEGQRRYVESLSAYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSRNPRSTVGT
                     ITEVYDYLRVLYARIGIPHCPKCGREIGRQTPDQIVDQVLDLPEETRFLVLAPIVRGR
                     KGEYEKLLGDLAKRGFSRARIDGEVRDLSEKIRLDRYYKHDIDVVVDRLVAKPDIRRR
                     VADSIETALELSEGIAAISIQTDAGEETITFSEALACTYDGLSFEELAPRNFSFNSPY
                     GACTTCDGLGTRLEVDPELVVPDPDRSLNEGAIAPWAGSRLEYWPRVLSSLAKTYGFS
                     MDTPWRDLPKDVRDLILYGSDDEIHVRYRNRYGRTRAYWTTYEGVIANQERRHEETDS
                     EFARSRIEQYMREVPCRACKGMRLRPESLAVTIGGRNIAELTGTSVGATVAFLEDLEA
                     ELSERERLIAERLLKEIRARLGFLLDVGLDYLTLDRGAGTLAGGEAQRIRLATQIGSG
                     LVGVLYILDEPSIGLHQRDNQRLIDTLVRLRDLGNTLIVVEHDEATIRVADWIVDIGP
                     GAGEAGGRIVYSGPVEGIEREEASLTGAYLSGRREIPTPVRRRRPDGRWLEIKGAAEH
                     NLKKIDVALPLGTFVCVTGVSGSGKSTLVQDVLSRALHSKLSRSRVVPGKHRRLVGWE
                     QLDKLVVVDQSPIGRTPRSNPATYTGVFDHIRRLFSQTPEARVRGYKPGRFSFNVRGG
                     RCEACAGDGQIKIEMHFLPDVYVTCEVCKGRRYNKETLAVRYKGRTIADVLGMSVEEA
                     LAFFRNIPVIARHMQTLADVGLGYVKLGQPAPTLSGGEAQRVKLASELSRRSTGRTVY
                     ILDEPTTGLHFEDVRKLLGVLERLVDAGNTVVVIEHNLDVIKTADWIVDLGPEGGDGG
                     GRLVAAGPPEEIARNPDSHTGRFLRPILASAQAAAGA"
     gene            complement(4388..5752)
                     /locus_tag="VM638_07635"
     CDS             complement(4388..5752)
                     /locus_tag="VM638_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HVM12329.1"
                     /translation="MAEPARPRERHDPTLIAAGPHLPRPRAGVILAAGRSERLAGLTG
                     GGSKALIRLGGMALVERAIRTLARLGVERIIVVVGYHAGPVAAVARRASPAVQVILAE
                     DWEEGNAASLAAAESAVAGAESFLLVTADHVFSAGALEALLDRGERSVLVDPAPSPEV
                     WAEGTRAEIDQQGHVVRLGKEVASGAVDCGAFVLDGEVFAAVRRARRAGDATLAGALS
                     LLPSLRAVSLPRRAWWWDVDTEEDLAAARRLLWGSLRRRGDGLVSRTLNRPLSTRLSW
                     AIAPLRPPPALLSWLSFAVVVAGASLVASGLGILGGILIQAGSVLDGVDGEVARLTLR
                     DGPGGAVLDGYLDRLSDAAVVLGLAVWALDRGGDPTAVVALAVAATAGAMLSMAMKDR
                     IAAQGLRAPSERLLDPLLSGRDARLLGVAILAMLAQPSIALGFIALTSLAASTARVAL
                     ARSR"
     gene            complement(5790..6827)
                     /locus_tag="VM638_07640"
     CDS             complement(5790..6827)
                     /locus_tag="VM638_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVM12330.1"
                     /translation="MGTLLIQARRAWLGRGRVLDDPAVVLDGGRIRWVGPARHAGAAD
                     QEVEGDWFLMPGPVDHHVHLGLSDPRAVLRGGVTAVRDLGWPPEDIFPLVDAASGPGF
                     EGPAVAACGPMITGRGGYPSRASWAPAGTALEVEGTEEAVAAVERVVAQDPAAIKVTL
                     NAEAGPTLTDDELVAIVDTAHRLARRVTCHVQGAGQTERALGAGVDELAHAPWTERLS
                     EAMIAGLARSTTIVGTLDIHSYGRSTPELEVAQDNLRRFRAAGGRILYGTDLGNGPIP
                     PGLHAQEARHLAACGLSNEEVLLAMTAHELVDDGRGDLIGLRADPLDDPAALRDPPLV
                     VRSGRIRRLDR"
     gene            complement(6904..>7925)
                     /locus_tag="VM638_07645"
     CDS             complement(6904..>7925)
                     /locus_tag="VM638_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12331.1"
                     /translation="CDGEPVPAGFEGVHVFDISDPADPALIGAVEFSEVGASHRGTAD
                     GCGSHTLTGVPDQANDRVIVYSNNSSGPSNKPICNTINVLEIPLGNPAAASHIGYVPL
                     MPGSRGSNNGCHDAGVILGEVNLLACASGHAANVFSIGGPRGGTLTSPRWLYNVEEVD
                     GAGNRVGVGGRWHSAAFTWDGEVLVLGWEPGGGSEPECQSTDPAIKKSMFFYDADTGA
                     KLGQWVLPRGQDGAGENCTVHNYNMVPLRSGRYVAVSGNYQAGTWITDFTNPASPVTV
                     AYSDPDPITPSNLGGAWSTYWYNGFLYESEIQSGLNVFRASDRRLASEIRLDHLNPQT
                     QLFSL"
BASE COUNT         1075 a         2908 c         2849 g         1093 t
ORIGIN      
        1 gtcgcagtgg aagtagtagc tgccggcctc gtcgaccgcc ggaacgtcgt acgtcacggt
       61 ctgcggaccc gcgaaggggt cctcccggaa gatcggctcc gacagggtgt cgtcggtgta
      121 gatcgcgacg ttgtgcaggg ccgtgtcgag gttctcgaac tcgatcgtca cgccctcccc
      181 caccggcgcg gcgaggcagt ccttgtcgaa ggcgatgttc tcggccacga tctggagctc
      241 ggtcccctcg ggctggcagg ccggggcggt cgtcggctcc tccgtcggct cgccggtcgg
      301 ctccgggccg gctggcgggg cggcgaggaa cggctcgtcg ccgccgcggg tgccgagctt
      361 gaccgcgctc atctgcgggt cgccccacgc gaggtgcacc gcgccctcgc cgccggacgc
      421 gacgctcggg gatccgccgc cggcgaactg cgggaaccgg tcggccgcga acgaaccttg
      481 ctcgttcgag gcgtagacga ccccctggtc gtcgctccag gcgacgtggt ggatgccctc
      541 gccgtccacc gcgatgcccg tgccgccggt cgccgcctcg cccacgtcgg tcgtctccca
      601 gtcaccgccc ccgatgctgt gcgagtgccg caccgccccc tgcgcgtcgc ggtaggcgag
      661 atgcggattg ccgtcaccgt cgagcgacat cgagacgccg atccccccgt cgtcgacgcc
      721 ctcagactcc cagacctcgc cgttgcggcg ggtgagcagc gtccggccct cgctgccgta
      781 cgcgacgagc gtcccctgtg cgcccgagcc gaccgccgtc gtcccgccct ccgggggcga
      841 ggcctcggca accgtctcga ccgaccacgc ggagccgtcg gaggtggcga gccgcaccgt
      901 ctgcgtctcc ccttgcgccg gctcctcgta gaacgtcacg agcggcgtct ccccctccag
      961 ggcgatcgag acaccggaga ccgtgccgtc gtgcacgacc gccggctcgg agaacgaccc
     1021 gccggcgttc gatgagtagc ggaccccggc gccgccctcg gtccaggcga cgtggtgcgt
     1081 cccgtcctcc ccgacggcga tcgcgaccgt gtcctccgcg gtcacgcccg tctcctcggc
     1141 cacggggctg tgggtccaga cgccgtcgac gacgtgcgcg tggaggaccg cagggggggt
     1201 cgggcccccg ggatccgggg ccgccgccgg ctccccctcc tcggcctcgg gctcgagggc
     1261 gatgtacgcg aggtgcgggt tgccctcggc gtcggtcgtc aggctcacac ccgcgtgacc
     1321 gatcccgggg ccggcgtcgg cgacctgcac gacgaaggcg ttggccggga cctcgccttc
     1381 ctgcaccgcg acgccgccct cgccgcacgc ggtcgcgagc agcgccagga gcgcgaacgg
     1441 gatcagcttc ctcgacacga cgccccgtgt tctatcacgc gagggcagcc accctccaac
     1501 tcaggcgccg gccgcggcct gcgccgaggc gaggatcggc cggaggaacc ggccggtgtg
     1561 cgagtccggg ttccgggcga tctcctccgg cggcccggcg gccacgaggc gcccccctcc
     1621 gtcgccaccc tccggcccga ggtccacgat ccagtcggcg gtcttgatca cgtccaggtt
     1681 gtgctcgatc acgacgacgg tgttccccgc gtcgacgagg cgctccagca cgccgagcag
     1741 cttgcggacg tcctcgaagt gcaggccggt cgtcggctcg tcgaggatgt agaccgtgcg
     1801 tcccgtcgag cgccgcgaga gctccgaggc cagcttcacg cgctgggcct ccccgcccga
     1861 cagcgtgggc gccggctgcc cgagcttcac gtagccgagc ccgacgtcgg cgagcgtctg
     1921 catgtgccgg gcgatcaccg ggatgttccg gaagaacgcg agcgcctcct cgacgctcat
     1981 ccccaggacg tcggcgatcg tccggccctt gtaccgcacg gccagcgtct ccttgttgta
     2041 gcgacgcccc ttgcagacct cgcaggtcac gtagacgtcg gggaggaagt gcatctcgat
     2101 cttgatctgg ccgtcgccgg cgcaggcctc gcaccggccg ccgcggacgt tgaacgagaa
     2161 gcgtcccggc ttgtagccgc gcacgcgcgc ctccggcgtc tgcgagaaca gccgccggat
     2221 gtggtcgaag acgcccgtgt aggtggccgg gttcgagcgt ggcgtgcgtc cgatcggcga
     2281 ctggtcgacg acgacgagct tgtcgagctg ctcccagccg acgaggcgac ggtgcttccc
     2341 ggggacgacc cgtgaccgcg agagcttgga atggagggct cgcgaaagca cgtcctggac
     2401 gagggtcgac ttcccggagc cggagacgcc cgtgacgcag acgaaggtcc cgagcgggag
     2461 ggcgacgtcg atcttcttca ggttgtgctc ggccgcgccc ttgatctcca gccatcggcc
     2521 gtcggggcga cggcggcgga ccggcgtcgg gatctcgcgc cgtcccgaca ggtacgcgcc
     2581 ggtgagcgac gcctcctcgc gctcgatgcc ctcgaccggg cccgagtaca cgatccgtcc
     2641 gccggcctcg cccgcgcccg ggccgatgtc gacgatccag tcggcgaccc tgatcgtcgc
     2701 ctcgtcgtgc tcgacgacga tcagcgtgtt gccgaggtcg cgcaggcgca cgagcgtgtc
     2761 gatcagccgc tggttgtcgc gctggtgcag cccgatcgac ggctcgtcga ggatgtagag
     2821 gaccccgacg agcccggagc cgatctgcgt ggcgagccgg atccgctgcg cctccccgcc
     2881 ggcgagggtc ccggcgccgc ggtcgagcgt caggtagtcg aggccgacgt cgaggaggaa
     2941 gccgagccgg gcgcggatct ccttcagcag gcgctccgcg atcagccgct cccgctcgga
     3001 gagctccgcc tcgagatcct cgaggaaggc gacggtcgcc ccgaccgagg tccccgtgag
     3061 ctccgcgatg ttccggccgc cgatcgtgac ggccaacgac tccggccgca ggcgcatgcc
     3121 cttgcacgcg cggcacggga cctcccgcat gtactgctcg atccgcgagc gcgcgaactc
     3181 ggagtcggtc tcctcgtgcc gccgctcctg gttcgcgatc acaccctcgt acgtcgtcca
     3241 gtacgcgcgc gtgcgcccgt agcggttgcg gtaccggacg tggatctcgt cgtcggagcc
     3301 gtagaggatc aggtcgcgca cgtccttcgg caggtctcgc cacggcgtgt ccatcgagaa
     3361 gccgtacgtc ttcgcgagcg aggagagcac gcgcggccag tactcgagcc gcgagccggc
     3421 ccacggcgcg atcgccccct cgttcagcga tcggtcgggg tcgggcacga cgagctccgg
     3481 gtccacctcg agcctcgtgc ccagcccgtc gcaggtcgtg cacgccccgt acggcgagtt
     3541 gaacgagaag ttccgcggcg cgagctcctc gaaggagagg ccgtcgtacg tgcaggccag
     3601 cgcctccgag aaggtgatcg tctcctcccc cgcgtcggtc tggatgctga tcgccgcgat
     3661 cccctccgag agctccaacg ccgtctcgat cgagtccgcg acgcggcggc ggatgtcggg
     3721 cttcgccacg aggcggtcga cgacgacgtc gatgtcgtgc ttgtagtagc ggtcgaggcg
     3781 gatcttctcc gagaggtcgc gcacttcgcc gtcgatccgc gcgcgcgaga acccccgctt
     3841 cgccagatcg ccgaggagct tctcgtactc ccccttccgg ccgcggacga tcggcgcgag
     3901 gacgaggaat cgtgtttcct cggggaggtc gagcacctgg tcgacgatct ggtcgggggt
     3961 ctggcgaccg atctcccggc cgcacttcgg gcagtgcggg attccgatcc gcgcgtagag
     4021 gacgcggagg tagtcgtaga cctcggtgat cgttccgacg gtcgaccgcg ggttccgcga
     4081 ggtcgacttc tggtcgatcg agatcgcggg cgagagcccc tcgatgaagt cgacgtccgg
     4141 tttctccatc tggccgagga actgccgggc gtaggcggac agcgactcca cgtatcgccg
     4201 ctgcccctcg gcgtagaccg tgtcgaacgc gagcgaggac ttcccggagc cggacaggcc
     4261 cgtgaacacg atcaggcggt cccggggaag ctcgaggtgg acgtccttga ggttgtgctc
     4321 acgggcgccg tggatgacga tccgatcggt gcccacgaga ggcatcgtac cagggggtgt
     4381 ggcttcctca gcgcgaccgg gcgagggcga cccgggcggt cgaagccgcc agcgaggtca
     4441 gggcgatgaa gcccagggcg atggacggct gggccaacat cgcgaggatc gcgaccccca
     4501 ggaggcgggc gtcgcggccg ctcaggaggg ggtcgaggag gcgctcggac ggcgcgcgca
     4561 aaccctgcgc cgcgatgcgg tccttcatcg ccatcgagag catcgcgccg gcggtggccg
     4621 cgacggcgag cgccacgacc gcggtggggt ccccgccgcg gtccagcgcc cagaccgcga
     4681 gcccgagcac gacggcggca tcggagagcc ggtcgaggta gccgtcgagg acggcgcctc
     4741 cggggccgtc ccgcagggtc agcctcgcca cctcgccatc gacgccgtcg agcaccgagc
     4801 cggcctggat caggatcccg ccgaggatgc ccagccccga ggcgacgagg ctcgcgcccg
     4861 cgaccaccac ggcgaacgag agccacgaca gcagggccgg gggcggccgg agcggcgcga
     4921 tcgcccacga gagccgggtc gacaggggac ggttgagcgt ccgagacacg aggccgtcgc
     4981 cacgacgccg aagggacccc cacaggagcc ggcgtgcggc ggcgaggtcc tcctcggtgt
     5041 cgacgtccca ccaccacgcc cgccgcggga gcgagaccgc gcgcagggac ggcaagaggc
     5101 tgagcgcgcc cgcgagcgtc gcgtcgccgg cccggcgagc acgacgaacg gcggcgaaga
     5161 cctcgccgtc gaggacgaac gccccgcagt cgacggcccc tgatgccacc tccttgccga
     5221 ggcggacgac gtgcccctgc tgatcgatct cggcgcgtgt cccctcggcc cagacctcgg
     5281 ggctgggcgc ggggtcgacc agcacggacc gctcaccgcg gtccagcagc gcttcgagcg
     5341 cccccgcgga gaagacgtgg tcggcggtca cgagcaggaa ggactccgcc cccgccaccg
     5401 ccgactccgc cgcggcgagc gaggcggcgt tcccttcctc ccagtcctca gccaggatca
     5461 cctgcaccgc cggggaggct cggcgcgcga ccgccgcgac gggcccggcg tgatacccga
     5521 cgacgacgat gatccgctcg accccgagcc tcgcgagcgt ccggatcgcc cgctcgacga
     5581 gggccatccc accgagccgg atcagcgcct tcgatccccc cccggtgagc cccgcgaggc
     5641 gctccgagcg tccggcggcg aggatcaccc ccgcacgggg acgcgggagg tgcggcccgg
     5701 cggcgatcaa cgtcggatcg tggcgctcgc gggggcgggc tggttcggcc atgggaggat
     5761 gctaccggag cgagcgaccg ggaaccgggt cagcggtcga gccgccggat gcggccgctc
     5821 cgcacgacca ggggtgggtc ccgcagcgcc gccggatcgt cgagcgggtc ggcccgcaga
     5881 ccgatcaggt cgccccgacc gtcgtccacg agctcgtggg cggtcatcgc gagcaggacc
     5941 tcctcgttcg acaggccgca ggccgccagg tgccgcgcct cctgggcgtg gaggcccgga
     6001 gggatcggtc cgttgccgag gtccgtcccg tagaggatcc gtccgcccgc cgcgcggaag
     6061 cgccggaggt tgtcctgcgc gacctcgagc tccggcgtcg accggccgta cgagtggatg
     6121 tcgagcgtgc ccacgatcgt ggtcgagcgc gcgagcccgg cgatcatcgc ctccgacagc
     6181 cgctcggtcc acggggcgtg cgccagttcg tccacgccgg ccccgagcgc ccgctccgtc
     6241 tgccctgccc cctggacgtg gcaggtcacg cgccgggcga gccggtgcgc cgtgtcgacg
     6301 atcgcgacga gctcgtcgtc ggtgagcgtc gggcccgcct ccgcgttcag ggtcaccttg
     6361 atcgccgcgg ggtcctgcgc gacgacccgc tcgaccgcgg ccaccgcctc ctccgtcccc
     6421 tcgacctcga gcgccgtccc ggcgggcgcc caggaggcgc ggctggggta gcccccgcga
     6481 ccggtgatca tcggcccaca cgccgcgacc gccggcccct cgaagccggg cccgctcgcg
     6541 gcgtcgacga gcgggaagat gtcttcgggc ggccagccga ggtcccggac cgcggtcacc
     6601 cccccgcgca gcacggcgcg agggtcggag aggccgaggt ggacgtggtg gtcgacgggc
     6661 cccggcatga ggaaccagtc cccctcgacc tcctggtccg cagcgccggc gtgacgagcg
     6721 gggccgaccc agcggatccg gccgccgtcc agcacgaccg ccgggtcgtc gaggaccctc
     6781 ccgcggccga gccacgcacg ccgggcctgg atcagcagcg tccccacccg cgaaccctac
     6841 cgccaggaac ggcgaagggc gggggccgcg cccccgccct tcctccgtgg atcccctcga
     6901 tgcctacagc gagaagagct gcgtctgcgg gttgaggtgg tccaaccgga tctcgctcgc
     6961 cagccggcga tcgctcgcgc ggaagacgtt gagaccggac tggatctcgg actcgtacag
     7021 gaacccgttg taccagtacg tcgaccacgc cccgccgagg ttgctcgggg tgatcgggtc
     7081 ggggtcggag tacgcgaccg tcaccgggct ggccgggttc gtgaagtccg tgatccacgt
     7141 gcccgcctgg tagttgccgc tgaccgcgac gtaccggccg ctgcggagcg ggaccatgtt
     7201 gtagttgtgg accgtgcagt tctcgccggc gccgtcctgt ccgcgcggca gcacccactg
     7261 gcccagcttc gcgcccgtgt cggcgtcgta gaagaacatg ctcttcttga tcgccgggtc
     7321 ggtcgactgg cactcaggct cggagccgcc gccgggctcc cagccgagga cgaggacctc
     7381 gccgtcccag gtgaaggctg cggagtgcca ccggccgccg acgcccaccc tgttgccggc
     7441 gccgtccacc tcctcgacgt tgtagagcca ccgcgggctc gtgagggttc cgccgcgcgg
     7501 ccccccgatc gagaagacgt tggccgcgtg gccgctcgcg caggcgagga ggttcacctc
     7561 gccgaggatg acgcccgcgt cgtggcagcc gttgttgctg ccgcggctgc cgggcatcaa
     7621 cgggacgtag ccgatgtggc tcgccgcggc cgggttgccg agcgggatct cgagcacgtt
     7681 gatcgtgttg cagatcggct tgttcgacgg acccgaggag ttgttgctgt agacgatgac
     7741 gcggtcgttc gcctgatcag ggacgcccgt cagcgtgtgc gagccgcagc catcggccgt
     7801 cccgcggtgg gaggcgccga cctcgctgaa ctcgaccgcg ccgatcagtg ccgggtccgc
     7861 ggggtcggag atgtcgaaca cgtgcacgcc ctcgaacccg gccggcaccg gctcgccgtc
     7921 gcaga
//
LOCUS       DATLGG010000266         2632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_121260, whole genome shotgun sequence.
ACCESSION   DATLGG010000266 DATLGG010000000
VERSION     DATLGG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121260"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VM638_07650"
     CDS             complement(<1..612)
                     /locus_tag="VM638_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12332.1"
                     /translation="MKPFDRKDWFRVARPATMSHFFRGREASLKRSILLISVLALTIL
                     GGAPATGSSSAWCSDTGGVGPCIVSVTRGGATITKTDATWQVEVHDFSDSTTKHFVWQ
                     VEKTGATPPSELGYSELGVEWVITLKAPGRPRTASFRGAGGEITRNAVVTSNEITFRG
                     SPVIVTDPPRETEECTHIWPWTCPHRASEEDVMLSGHVSDQGQY"
     gene            complement(806..>2632)
                     /locus_tag="VM638_07655"
     CDS             complement(806..>2632)
                     /locus_tag="VM638_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12333.1"
                     /translation="SVVMRARTLVAVVLFLPLVPAAPLRAAATTGGAGTPEVLISGLG
                     DARALAVRPDGDLFVADRSRILRRAGDGRTEVVAGSTQPGDNDGHGSEARFRMITGLA
                     FDDAGNLWIADHGAGRVKVLDPTGDVATVAGSGVTNNPNGPGGPARLEPLFEPSDLVP
                     MPDGSVIFTQGGADYIWPGRHGSQATAVAPIGRLRAIRDGDVVNLNREAYPPYMLPND
                     GRLQDANFSFPRGIARGPDGAVYIAEWASNRIRRVEGNTVETVYGDGGRGNRGDGSLA
                     VLASIHHPLDLAFGPEGHLFITQGHSPERPGGGTGQGGDDPQVRMVSPSGMIQRITSP
                     SAPIYQPLSVDVMPDGSVVFVNRRCVNWCGGGEDVAVAQVLRIPKPEGGWPNPAPNVV
                     CDPYIADGTGDARQRLVSSNRGVNRPWLDIVGAEIRTLGDRLEAQIRVEDLGEAWRGT
                     YAGLGTTNAWFVILRVGDDRYRYLLASTPANLESADATALVHFEYGSAGGVQEALRWD
                     WWYNETWYTDRAGSATGTLDPATDTITISAPLAGLGLKLGDTITYTEAMGGSLFEWRL
                     GRRSAWGDDKAPAPADFESTGTGGTFRSIRVGRNCPEGYLGA"
BASE COUNT          433 a          901 c          905 g          393 t
ORIGIN      
        1 gtactgaccc tggtcgctca cgtggccgct gagcatcaca tcttcctcgc tcgcccggtg
       61 agggcaggtc caaggccaga tgtgggtgca ttcctccgtc tcccgcggag gatccgtgac
      121 gatgacggga ctgccccgga aggtgatctc gttggaggtc accacagcgt tccgagtgat
      181 ctcaccgccg gctccgcgga acgaggccgt gcgagggcgt ccgggtgcct tcagtgtgat
      241 gacccattcg acgccgagct cgctgtaccc cagctcgctc ggcggtgtcg ctccggtctt
      301 ctccacctgc cacacgaaat gcttcgtcgt cgagtctgag aagtcatgga cctcgacctg
      361 ccaggtcgca tcggtcttgg tgatcgtagc cccgccgcgg gtgaccgaga cgatgcacgg
      421 cccgacccct ccggtatcgg aacaccacgc gctgctcgaa cctgtggcgg gcgctccccc
      481 caggatcgtc agggccagaa cggatatgag caggatcgag cgcttcaagc ttgcctccct
      541 ccctcggaaa aagtggctca tcgtagcagg gcgtgccacg cggaaccagt ctttccggtc
      601 gaacggcttc accgggtctc ggcggaacat ccggacccgc cgaggcgccg ccgcgactcc
      661 ggcgatcgtg atcgcggtag gatcacccca tggcgaacaa ggacaagggc ggggccaaga
      721 acagcaagaa ggtggcgaag cagagcctca aggaaaagcg ccaagccaag aagctcaaga
      781 accccaagta gcgcgcgcta gccccctagg ctcccaggta cccctcgggg cagttgcgcc
      841 cgacgcggat cgagcggaac gtgccaccgg tgcccgtcga ttcgaagtcg gccggtgcgg
      901 gagccttatc gtcgccccat gcggatcgcc gcccgagacg ccactcgaag agcgacccac
      961 ccatcgcctc ggtgtaggtg atcgtgtcgc cgagcttcag cccgaggcca gcgagcggag
     1021 ccgagatcgt gatcgtatcc gtggcggggt cgagcgtgcc ggtcgcggaa ccagccctgt
     1081 ccgtgtacca ggtctcgttg taccaccagt cccagcgcag cgcctcctgc actcccccgg
     1141 ccgagccgta ctcgaagtgg acgagcgccg tcgcgtccgc tgattcgagg ttcgcgggcg
     1201 tggaggcgag gaggtagcgg taccgatcgt cccccacgcg gaggatcacg aaccaggcgt
     1261 tggtcgtgcc gaggccggcg taggtgccgc gccaggcttc gccgaggtcc tcgacgcgga
     1321 tctgggcctc gagacggtcc ccgagggtgc ggatctcggc gccaacgatg tcgagccacg
     1381 gccggttgac cccgcggttg gacgaaacga gtcgctgacg agcatccccg gtgccatcgg
     1441 cgatgtaggg gtcgcacacg acgttcggcg ccgggttcgg ccagcccccc tccggcttcg
     1501 ggatccgcag tacctgagcg acagcaacgt cttcgccacc gccgcaccag ttcacacacc
     1561 ggcggttcac gaagaccacg gagccatccg gcatgacgtc gaccgacagc ggctggtaga
     1621 tgggggcgga aggggatgtg atccgctgga tcatcccgct cggcgagacc atgcgtacct
     1681 gggggtcgtc accgccttgg ccggtcccgc cgccgggacg ctccgggctg tgcccctggg
     1741 tgatgaagag gtgtccctcg gggccgaagg caagatccaa cggatggtgg atcgaggcga
     1801 gaaccgccag cgagccgtcg ccgcggttcc cccggccgcc gtcgccgtag accgtctcga
     1861 ccgtgttccc ctcgacacgc cggatccggt tgctcgccca ctccgcgatg taaacggcgc
     1921 cgtcagggcc ccgcgcgatc ccgcgcggga acgagaagtt ggcatcctga agccggccgt
     1981 cgttcggcag catgtaaggc gggtaggcct cgcggttgag gttcacgacg tctccatcgc
     2041 ggatcgcccg caggcgcccg atcggggcaa cggcggtggc ctgggatccg tggcggccag
     2101 gccagatgta gtcggcgccg ccctgagtga agatgacgga gccgtccggc atcggcacga
     2161 ggtcgctcgg ctcgaacaga ggctcgaggc gagccggccc gccagggccg ttggggttgt
     2221 tcgtgacgcc ggatccggcg acggtcgcaa cgtcgcccgt gggatcgagg accttgacgc
     2281 gtcccgctcc gtggtcagcg atccacaggt tcccggcgtc gtcgaacgcc aggccggtga
     2341 tcatccggaa gcgcgcctcg cttccgtggc cgtcgttgtc ccccggctgg gtcgagccgg
     2401 cgacgacctc ggtcctgcca tcgcccgccc gacggaggat gcgcgaccgg tcggctacga
     2461 acaggtcccc gtcgggccgt acggccagag cacgagcgtc gccgaggccc gagatcagga
     2521 cctcaggtgt cccggcgcct ccggtcgtcg ccgcggcacg gagcggcgcg gcgggaacca
     2581 gcggcaggaa cagcacgacc gcgacgagag tgcgagcgcg catcacgaca ga
//
LOCUS       DATLGG010000267         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_104399, whole genome shotgun sequence.
ACCESSION   DATLGG010000267 DATLGG010000000
VERSION     DATLGG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104399"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VM638_07660"
     CDS             complement(<1..692)
                     /locus_tag="VM638_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12334.1"
                     /translation="MNGVLLLWGESDYVLRLAAREAFGDERPAEVDAAEWRAGATGDL
                     ATPSLFGERRGLLITSAQDLPAEAIEEVARYAEAPDPDALLVLAATTGDRAKSPPAKL
                     ARPLKDRAEVRRVALDRRELPAWLAGRARARGIAATPDGLRQLVEVLGDDPAALDQAI
                     EQLGSAFPSEGVTPATVARQLRGFGDRRIYELCDAAFARDPVTAHRYLRSMLDAREEP
                     LAILGGIASRLR"
     gene            complement(689..3040)
                     /locus_tag="VM638_07665"
     CDS             complement(689..3040)
                     /locus_tag="VM638_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HVM12335.1"
                     /translation="MSPSALPLAAAAVAGGLLAGPALLPRVSAVPVLVAGLAGLTFAR
                     RRRRHASPIETSGLLGERERILAAAGFGTAPRARDGSVRLVALVLALVVAGAGWGALR
                     RARPERSGLPDGSYVRFEGSLKSDPRGFAFGWGAEAEVRPLLRDSPTDRSARVWIRGR
                     DRPPSLEAGSRIVGGGTVRLLRHGASSFADHLLSRGVVATLSVRRLERAPGAPPAPLR
                     LANAIRRVLRRGAETALPAREAGLLLGLSIGDVRTMDPEVEHDFRATGLGHLVAVSGS
                     NVAMVLAPVLALATLLRAGPAVRTLVGATAVGVFALVTRWEPSVLRASVMAVLGLLGV
                     LAGRPRSTMTILGGAVLGLLVLDPGLVRSLGFQLSVLATVGLVTLAGPLADRAAWMPR
                     PIALALGATLGAQIAVAPLLLSTFGVVPLASVPANLLALPLVMPALLLGILAAAAAVL
                     IPPVGFVLGRLGAVPLAVLCDVSDRVAKAGFPSVTGGLRAGVVAGALALVLGAFVRRG
                     SHRWRRMAALVTTLGLVAWAGGRAGPPPVLTVTFFDVGQGDGALVRTPEGVNVLVDAG
                     PERDLVATRLAALGVRRLDLAVSSHGHADHIDGFPAVLARHRVALLLDPMCAAESPSY
                     EALRRAAAAEDVTMRPARGGTTYRLGRLVLEILGPDRCSDEGPNDDSVVLRLRYGEAT
                     ILFPGDAEHAAQQDLLDDPDPVAADVLKVPHHGGDTSLDTFFSAVGARVAVVSTGPNT
                     YGHPVPSVLAALRATGATVLRTDRLGDVELRFEPEGIVVGSRG"
     gene            complement(3037..3585)
                     /locus_tag="VM638_07670"
     CDS             complement(3037..3585)
                     /locus_tag="VM638_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVM12336.1"
                     /translation="MAVAVALALAAGGIWYGRSAPVPAAVIETGPSPEPGPSPEPARL
                     FVHVAGWVERPGVYELPEGSRVNEAIELAGGAKRGADLSVLNLAAVLVDAQQVLVPKR
                     GETPGPAGPPGAAPGAEALINLNTATLEQLDTLPGIGPVLAQRIIDFRDQNGPFTSVD
                     QLTEVSGIGEKRFADVKNRVTV"
BASE COUNT          552 a         1449 c         1434 g          394 t
ORIGIN      
        1 tcgcggagcc gcgaggcgat gccgccgagg atcgcgaggg gctcttctcg tgcgtcgagc
       61 atgctgcgga ggtagcggtg cgccgtgacc gggtcgcgcg cgaacgccgc gtcgcacagc
      121 tcgtagatcc gccgatcgcc gaagccgcgg agctggcgag cgaccgtcgc gggtgtcacg
      181 ccctcgctcg ggaaggcgga gccgagctgc tcgatagcct ggtcgagcgc cgccgggtcg
      241 tcgccgagga cctcgacgag ctgacggagc ccgtcggggg tcgccgcgat cccccgggca
      301 cgtgcccggc cggcgagcca ggccgggagc tcgcggcggt ccaacgccac ccgtcgcacc
      361 tccgcccggt ccttcagcgg gcgggccagc ttcgccggcg gcgacttcgc ccggtccccc
      421 gtcgtcgccg cgaggacgag cagcgcgtcc gggtcgggcg cctcggcgta gcgcgcgacc
      481 tcctcgatgg cctccgccgg aagatcctgg gcggaggtga tcagcaggcc gcgtcgctct
      541 ccgaagagtg agggggtcgc caggtcgccg gtcgcgcccg ctcgccattc ggccgcgtcc
      601 acctcggcgg gccgctcgtc cccgaacgcc tcccgagcgg cgaggcgaag cacgtagtcg
      661 ctctcgcccc acagcaggag gacgccgttc atccccgcga tcctacgacg atcccctccg
      721 gctcgaaccg cagctcgacg tcgcccagcc gatccgtgcg caggacggtc gcaccggtgg
      781 cccggagcgc ggcgagcacc gacgggaccg ggtgcccgta ggtgttcggc cccgtgctca
      841 cgacggccac ccgcgccccc accgcgctga agaacgtgtc gagggaggtg tcgccgccgt
      901 ggtgcgggac cttcagcacg tcggccgcga cgggatcagg gtcgtcgagg agatcctgct
      961 gggcggcatg ctccgcgtcg cccgggaaga ggatcgtcgc ctccccgtag cggaggcgga
     1021 gcacgacgga gtcgtcgttc gggccctcgt cggagcagcg gtcgggaccg aggatctcca
     1081 gcacgagtcg gccgaggcga taggtcgtgc ccccgcgggc cgggcgcatc gtcacatcct
     1141 cggcggcggc cgcgcgccgg agggcctcgt aggaggggga ctcggcggcg cacatcgggt
     1201 cgagcagcag ggcgacgcga tgtcgcgcca gcaccgccgg gaacccgtcg atgtggtcgg
     1261 catggccgtg cgaggagacg gcgagatcca gccgccggac gccgagcgcg gcgagacgcg
     1321 tcgcgacgag gtcgcgctcg ggtcccgcgt cgacgaggac gttcaccccc tccggggtcc
     1381 ggacgagcgc gccgtcgccc tggccgacgt cgaagaacgt gacggtgagg accgggggcg
     1441 gtcccgcccg gccgccggcc caggccacga gccccagggt cgtgacgagg gcggccatgc
     1501 ggcgccaccg atgcgaccct cgccgcacga acgcgccgag gacgagcgcg agcgcccccg
     1561 cgacgacccc cgcgcggaga ccaccggtga ccgaggggaa gcccgccttc gcgacgcggt
     1621 ccgagacgtc gcacaagaca gcgaggggca ccgcgccgag gcgaccgagc acgaacccca
     1681 ccggtgggat gagcaccgcc gccgccgccg cgaggatccc gagcagcagc gccggcatca
     1741 cgagggggag ggcgagcagg ttcgccggca ccgaggcgag cggaacgacg ccgaaggtcg
     1801 acagcagcag cggagcgacg gcgatctgcg cgccgagcgt cgctccgagc gcgagcgcga
     1861 tcggacgcgg catccatgcg gcgcggtccg cgagcggccc cgcgagcgtg acgagcccaa
     1921 ccgtcgcgag gacagagagc tggaacccga gcgagcgaac gaggccggga tcgaggacga
     1981 gcaggccgag gacggcgccc ccgaggatcg tcatggtgct gcgcgggcgt cccgcgagga
     2041 cgccgaggag cccgagcacc gccatgacgc tggcgcggag aacggagggc tcccagcgcg
     2101 tcaccagcgc gaagacgccg acggccgtcg cgccgacgag cgtccggacg gccgggccgg
     2161 cgcgcagcag cgtcgcgagg gcgagcacgg gcgcgaggac catcgccacg ttcgatcccg
     2221 agaccgccac gaggtggccg aggcccgtgg cgcggaagtc gtgctcgacc tccggatcca
     2281 tcgtgcgcac gtcgccgatg ctcagcccga gcaggaggcc ggcctcccgc gcgggcagcg
     2341 cggtctccgc tccacgccgc agcacgcggc ggatcgcgtt cgccaggcgg aggggcgccg
     2401 gtggagcccc cggcgcgcgc tcgaggcggc gaaccgagag ggtcgcgacg accccacgcg
     2461 aaagcaggtg gtccgcgaac gaggacgcgc cgtgccgcag caggcggacg gtcccgcctc
     2521 cgacgatgcg gctcccggcc tcgagcgacg gcggccggtc ccgcccccgg atccagaccc
     2581 gcgccgaccg gtccgtcggg gagtcccgca gcagcggtcg cacctcggcc tccgcgcccc
     2641 acccgaacgc gaacccgcgt gggtccgact tgagcgaccc ctcgaaccgc acgtagctcc
     2701 cgtcgggaag gccgctccgc tccgggcgcg cccgacggag ggctccccag cccgcaccgg
     2761 cgacgacgag cgccagcacg agcgcgacca gccgcaccga cccgtcccgg gcccgtggag
     2821 cggtgccgaa cccggcggcc gcgaggatcc gctcgcgctc gcccagcaag ccgctcgtct
     2881 cgatcggcga cgcgtgccgg cggcggcgcc gggcgaacgt tagaccggcg agtccggcga
     2941 ccagcacggg aaccgcggac accctcggca gcagcgccgg gcctgcgagg aggccccccg
     3001 cgacggcggc cgctgcgagc gggagcgcgg acgggctcac acggtgacgc gattcttcac
     3061 gtccgcgaag cgcttctcac cgatgccgct cacctccgtg agctgatcca cgctggtgaa
     3121 cggcccgttc tggtcccgga agtcgatgat gcgctgggcg agcacgggcc cgatccccgg
     3181 caaggtgtcg agctgctcga gcgtcgccgt gttcaggttg atgagcgcct ccgctccggg
     3241 ggctgccccg ggtgggccgg ccggcccggg ggtctcgccg cgcttcggca cgaggacctg
     3301 ctgggcatcg acgagcaccg ccgcgaggtt cagcacgctc aggtcggctc cccgcttcgc
     3361 gccacccgcc agctcgatcg cctcgttgac gcgcgatccc tcggggagct cgtagacgcc
     3421 cggacgctcg acccagccgg cgacgtgcac gaagaggcga gcgggctccg ggctcggtcc
     3481 cggctcgggc gagggtcccg tctcgatcac cgccgcgggc accggcgccg aacgtccgta
     3541 ccagatcccg cccgccgcga gcgcgagcgc caccgccacc gccacggcag cccccgttcg
     3601 cgcgccgcga gcgctcaacc gatcccgcca ccgatcgtcc acacgaccac ctccctcgac
     3661 gacgcaacct cgggggaggc gtcgagcacg ctagcaggga ccaccgacac gccagcgggt
     3721 agggtctggc cccgtgggac gacccggccg gatcgacgct tccgcctcgg cgctcgtggc
     3781 cgctggactg tggggcggca tgtacgtcat cagcaaggac accttcgcg
//
LOCUS       DATLGG010000268         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_10092, whole genome shotgun sequence.
ACCESSION   DATLGG010000268 DATLGG010000000
VERSION     DATLGG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10092"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VM638_07675"
     CDS             <1..456
                     /locus_tag="VM638_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12337.1"
                     /translation="VTQENGYPGRPRHAIPTLVFALAVVVAVGSTSGLIWFLFQRTEG
                     PAPVLRTFAERVRDGDCPGSYRLLEASTQATIAPEDWCELLEPVQAQLDPGFRVTEMR
                     LREDIAELSVRGRLSAEPSVWRLRKAGRSWRVLGPTGGFEALLEQSPAA"
     gene            complement(453..2183)
                     /locus_tag="VM638_07680"
     CDS             complement(453..2183)
                     /locus_tag="VM638_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVM12338.1"
                     /translation="MRLIRRTTLIVLAAALVAPPTVASAAPHPDADHTEAYFPANDGL
                     GTKLHADILRPKGMSWDVKTPVVMTVSPYTNHGGVPTVDPVLGHVNGSGPSERFYDFL
                     SVSQILKKGYTYVMVDLPGFGGSGGCNDWGGNREQGAVKAAVEWAASEPWSTGKVALM
                     GKSYDAWTGLMGVAQRPNGLAAVVAMEPVYAGYRYGYNNGVRFTNSVLTPALFTAFDV
                     QPGPVTVSPDYQVNGAPQAWCYGVNLGMQQIDDPNDPFWRERDLIPDVIGERTPVFLT
                     QGFLESNTKPDGAFEFWNNLDLRHGRAWFGQFGHWRGWERNGQGRFYMGRSVFAAEMM
                     RFLDHHLKGLETGVEEDPRVAVQDNRGRYRAETQWPPEDMTMWWNDLNTGSYRDDLTV
                     SSTPVWSFSQILPHDVWLSGEPVLRVEVDAPAPRANLVADVFAYNPANGRATLINRGA
                     YLLRGRGTQRATFKLYGQDWRIPAGHRIGVAISDTDVRASNWWAHVPTGQTVTVRSAK
                     IGLPFLYWERDPEEYLDGGSTPDLESHLGGTVSVSASAAAAAARDFDLPERLKPRPKD
                     EDEQAEATAG"
     gene            complement(2302..>2881)
                     /locus_tag="VM638_07685"
     CDS             complement(2302..>2881)
                     /locus_tag="VM638_07685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HVM12339.1"
                     /translation="DVAARLGDLLGELGHDEVTFTIVGSGPNGASPHHEPGDRVLQAG
                     DAVVMDFGGRAGGYCSDITRTVGVREIADEIREVHEIVREAQERAFHAVRPGVPAQEV
                     DRAAREVIASAGYGERFIHRTGHGIGLEEHEPPYLVEGNEDPLLAGMCFSIEPGIYLP
                     GRFGVRIEDIVTVTDDGARRLNRANRELELVG"
BASE COUNT          385 a         1034 c         1007 g          455 t
ORIGIN      
        1 gtgacccagg agaacggata ccccggccgg ccgcgccacg cgatcccgac gctcgtcttc
       61 gccctggcgg tcgtcgtggc ggtggggtcc acctccgggc tcatctggtt cctgttccag
      121 cgcacggaag gcccggcgcc ggtcctgcgc acgttcgccg agcgcgtgcg tgacggcgac
      181 tgcccgggga gctatcgcct cctcgaggct tccacgcagg cgacgatcgc gccggaggac
      241 tggtgcgagc tgctcgagcc ggtccaggcc cagctcgacc ccgggttccg cgtcaccgag
      301 atgcggctcc gcgaggacat cgcggagctt tccgtgcgcg ggcgtctctc cgccgagccc
      361 agcgtctggc ggttgcgcaa ggccgggcgg agctggcgcg tgctcggtcc gaccggcggg
      421 ttcgaggccc tgctggagca gagcccggcc gcctaaccgg ccgtcgcttc ggcctgctcg
      481 tcctcgtcct tcggtcgtgg cttgagccgc tcgggcagat cgaagtcccg ggcggccgcc
      541 gcggcagctg aggcgctgac cgagaccgtg ccgccgaggt ggctctcgag gtcgggtgtc
      601 gacccgccgt cgaggtactc ctccggatcg cgctcccagt acaggaacgg cagaccgatc
      661 ttcgccgagc gcaccgtcac ggtctggccg gtcggaacgt gcgcccacca gttgctggcc
      721 cggacgtcgg tgtccgagat cgccaccccg atccggtggc cggcggggat ccgccagtcc
      781 tgaccgtaga gcttgaaggt ggcgcgctgg gtcccgcgcc cacgcaggag gtaggcgcca
      841 cggttgatga gcgtggcccg gccgttcgcc gggttgtagg cgaagacgtc ggcgacgagg
      901 ttcgcccgcg gagccggcgc gtcgacctcg acccggagga ccggctcacc ggacagccac
      961 acgtcgtgag gaaggatctg ggagaacgac cagaccggcg tgctgctcac cgtcaggtcg
     1021 tcgcggtagg aaccggtgtt gaggtcgttc caccacatcg tcatgtcctc cgggggccac
     1081 tgcgtctccg cccggtagcg cccgcggttg tcctggaccg cgacccgggg gtcctcttcg
     1141 acgcccgtct cgaggccctt caggtggtgg tcgaggaaac gcatcatctc ggctgcgaag
     1201 accgaccggc ccatgtagaa gcggccctgc ccgttccgct cccatccccg ccagtggccg
     1261 aactggccga accacgcccg cccgtgccgc aggtcgaggt tgttccagaa ctcgaaggcg
     1321 ccgtccggct tcgtgttcga ctccaggaac ccctgggtca ggaagaccgg cgtccgctcc
     1381 ccgatcacgt cggggatcag gtcacgctcg cgccagaagg ggtcattcgg gtcgtcgatc
     1441 tgctgcatcc ccaggttgac gccgtagcac cacgcctgtg gagcgccgtt cacctggtag
     1501 tcggggctca ccgtcacggg gcccggctgc acgtcgaacg ccgtgaagag cgccggcgtg
     1561 agcaccgagt tcgtgaagcg aacgccgttg ttgtagccgt agcggtaccc ggcgtagacc
     1621 ggttccatcg cgacgaccgc ggccaggccg ttcggccgct gagcgacgcc catcaacccg
     1681 gtccaggcgt cgtacgactt gcccatgagg gcgaccttgc ccgtggacca cggctcggag
     1741 gccgcccact cgacggccgc cttcaccgct ccctgctcgc ggttgccgcc ccagtcgttg
     1801 cagccgcccg acccgccgaa ccccggcagg tcgaccatga cgtaggtgta gcccttcttc
     1861 aggatctggc tgacgctcag gaagtcgtag aagcgctcgc tcgggccgct cccgttgacg
     1921 tggccgagga cggggtcgac cgtcgggacc ccgccgtggt tcgtgtacgg gctgacggtc
     1981 atgaccacgg gggtcttcac gtcccacgac atccccttgg gacgcaggat gtcggcgtgc
     2041 agcttcgtgc cgaggccgtc gttggcgggg aagtaggcct ccgtgtggtc ggcgtcgggg
     2101 tgcggcgcgg cgctcgccac ggtggggggc gccaccagcg cggcggccaa tacgatcagg
     2161 gtggtccgtc ggatcaggcg cacgatccct ccaggcgtcg cgggtcaccc tcggtccttc
     2221 cggggcgtcg cgttggtatt cgccgcgcgc agtggcgaat cctccccgcc gtctccggcc
     2281 ggcgggcgaa ccgccggacc ttcagccgac gagctcgagc tcgcggttcg cccggttcag
     2341 ccgtctcgca ccgtcatcgg tgacggtgac gatgtcttcg atccggacgc cgaagcggcc
     2401 cggaaggtag atccccggct cgatcgagaa gcacatcccg gcgagcaggg gatcctcgtt
     2461 cccctcgacg aggtacgggg gctcgtgctc ctccaggccg atgccgtggc cggtccggtg
     2521 gatgaagcgc tcgccgtacc cggcggaggc gatcacctcg cgcgcggcgc ggtcgacctc
     2581 ctgcgctggg acgcctggcc ggaccgcgtg gaacgctcgc tcctgagctt cacggacgat
     2641 ctcgtggacc tcccggatct cgtcggcgat ctcccggacg ccgaccgttc tcgtgatgtc
     2701 ggagcagtag ccacccgctc gcccgccgaa gtccatcacc accgcatcgc ccgcctggag
     2761 cacgcgatcg cccggctcgt ggtgcggcga ggccccgttc ggccccgacc cgacgatcgt
     2821 gaaggtcacc tcgtcgtggc cgagctcgcc gagaaggtcg cccaggcgcg cggcgacgtc
     2881 c
//
LOCUS       DATLGG010000269         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_76609, whole genome shotgun sequence.
ACCESSION   DATLGG010000269 DATLGG010000000
VERSION     DATLGG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76609"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VM638_07690"
     CDS             <1..474
                     /locus_tag="VM638_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HVM12340.1"
                     /translation="GARVSGALADQVPLVEALDPPPDVIVTEIGANDAIRLTGGRRFR
                     RDYDRLLERVDALGARHVVCLGLPSFSSTPRFLQPLRAIIAWRARVLDAEVRASAAEH
                     GAHYVDIAAATEPAFDADPEGTHAADDFHPNDAGYRAWARAVLSVLRPLVTGSSG"
     gene            522..1709
                     /locus_tag="VM638_07695"
     CDS             522..1709
                     /locus_tag="VM638_07695"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVM12341.1"
                     /translation="MKEIAPNLRSWASEIDDLTVKQAIAASRLPILAGPVALMPDAHL
                     GKGATVGSVIPTDSAIIPSAVGVDIGCGMIAVETDRRLDQVPDVLERILERFSEGIPA
                     GLGRWHGRASEPARRWLRDHPHPSLTPEQEEKALVQFGSLGSGNHFVELAADERDIVW
                     IVMHSGSRGVGNQLATKHIALAKKQHQALEDPDLAYFLEGTPGFQAYVRDMLWAQEYA
                     MANRRALMSAALEAFGAVTGGGGAAAPWINCHHNYAALEEHDGRKMWVTRKGAIRAGR
                     GDLGVIPGSMGSKSYIVRGRGNPLSYESSAHGAGRRLSRGRARRELTLEDFTRAMSGR
                     VWQARDAEALVDEAPQAYKDIDQVMADQADLVEIVHELRGILSYKGVESPRARGKRRS
                     KRG"
     gene            complement(1725..>2948)
                     /locus_tag="VM638_07700"
     CDS             complement(1725..>2948)
                     /locus_tag="VM638_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12342.1"
                     /translation="GQTNCVPLPANAGACGDIFVRDRSKNITERVSVSSEGVEGDADS
                     GSPRISPEGRYVGFISSAANLVEGDANDHSDVFLHDRETNITERVSVSSAGAQPDDWS
                     VSPPGVSEGGRWVAFQSRATNLVPGDTNGPGLAGEDVFVRDRGPDLGFGDLTVSRRQD
                     GSSVSGWVTFPAAIVSSATDADDDADIGPADGRRLAAELLGATVTYRPEREDLLLRTH
                     LANDGEAPPSAGALHGAAMVFGFDFTVGGTAWSVRGERPSGTQTAPAAARFTLWRCDP
                     TCSEVGTLPGSLGTTASEALVAVPLGSIGAFEGAPLSDLRAFTSLATAATGAPTEVDL
                     VALPSGAIPRRTLSLGVAPPGTAEEDVVTDQAIPVLEGRFTATIHQPSGEGQAVWARA
                     CLGMRCEVASRRLDR"
BASE COUNT          440 a         1055 c         1045 g          408 t
ORIGIN      
        1 ggcgcacgcg tctcgggggc gctcgccgac caggtcccgc tggtcgaggc gctcgacccg
       61 ccgccggacg tcatcgtcac ggagatcggg gcgaacgacg ccatccgcct cacgggagga
      121 cgccggttcc gtcgggacta cgatcggctg ctcgagcgcg tcgacgcgct cggtgcccga
      181 cacgtggtct gcctcgggct cccgtcgttc agcagcacgc ccaggttcct ccagccgctc
      241 cgcgcgatca tcgcctggcg cgcccgcgtg ctcgatgccg aggtccgggc ctcggccgcc
      301 gaacacggag cccactacgt ggatatcgcg gccgcgaccg agccggcgtt cgacgcggat
      361 cccgagggaa cgcacgccgc cgacgacttc catccgaacg atgcggggta ccgcgcatgg
      421 gcgagagccg tgctctccgt cctccgtcca ctggtcacgg gctcgagcgg atgaggcagg
      481 aagcgtggcg cgcccgtcgc tcggcaacca gactggcagg tgtgaaggag atcgcgccga
      541 accttcggag ctgggcgtct gagatcgacg acctgacggt gaagcaggcg atcgcggcca
      601 gccgcctgcc gatcctcgcg gggccggtcg cgctcatgcc ggacgcgcat ctcggcaagg
      661 gagcgaccgt cggctcggtg atcccgaccg actccgcgat catcccctcg gccgtcggcg
      721 tcgacatcgg ctgcgggatg atcgccgtcg agacggaccg ccggctcgac caggtccccg
      781 acgtcctcga gcgcatcctc gagcggttca gcgagggcat cccggcgggg ctcgggcggt
      841 ggcacggacg ggcgtccgag cccgcacgcc gatggctccg cgaccacccg cacccgtcgc
      901 tgacgccgga gcaggaggag aaggcgctcg tgcagttcgg ctccctcggt tccggcaacc
      961 acttcgttga gctcgccgcc gacgagcgcg acatcgtctg gatcgtgatg cactccggat
     1021 ctcgcggcgt cgggaaccag ctcgccacga agcacatcgc gctcgcgaag aagcagcatc
     1081 aggcgctcga ggatcccgac ctcgcgtact tcctcgaagg gaccccgggg ttccaggcat
     1141 acgtccgcga catgctctgg gcgcaggagt acgcgatggc gaaccgtcgg gccttgatga
     1201 gcgccgcgct cgaggcgttc ggcgcggtga cgggcggggg aggcgccgcc gcgccgtgga
     1261 tcaactgcca ccacaactac gcggcgctcg aggagcacga cggccgcaag atgtgggtca
     1321 cgaggaaggg tgcgatccgt gccgggcgcg gcgacctcgg ggtgatcccc gggtcgatgg
     1381 gatcgaagtc gtacatcgtg cgcggccgcg ggaacccgct ctcgtacgag tcgtcggcgc
     1441 acggcgccgg tcgtcgcctg agccggggcc gagcccgccg ggagctgacc ctcgaggact
     1501 tcacgcgggc gatgtcgggc cgcgtgtggc aagcgcgcga cgcggaggcg ctcgtcgacg
     1561 aggcccccca ggcgtacaag gacatcgacc aggtgatggc cgaccaggct gacctcgtgg
     1621 agatcgtcca cgagctccgc gggatcctca gctacaaggg cgtcgagtct ccgcgagcgc
     1681 gcgggaagcg tcgcagcaaa cgcgggtgag gccggccccg gccttcagcg atcgagtcgg
     1741 cgcgacgcga cctcgcagcg catccccaga cacgcacgag cccagaccgc ttgtccctcg
     1801 ccggatggct gatggatggt tgcggtgaac cgtccttcca ggacggggat cgcctggtcg
     1861 gtcacgacgt cctcttctgc cgttcccggc ggcgccacgc cgagcgacag cgtccgcctc
     1921 gggatggcgc ccgacggcag tgccacgagg tcaacctcgg tcggtgcgcc cgtcgctgcg
     1981 gtggcgaggc tcgtgaaggc gcggaggtcc gacagcggag ccccctcgaa cgcgccgatc
     2041 gacccgaggg gcactgccac cagcgcctcc gacgccgtcg ttccgaggct ccccggaagc
     2101 gtcccgacct cggagcacgt cgggtcgcag cgccagaggg tgaaccgcgc ggccgccggc
     2161 gccgtctgcg tcccgctggg tcgctcgccg cgaaccgacc aggcggtccc gccgaccgtg
     2221 aagtcgaatc cgaagaccat cgcggccccg tgcagcgcac ccgcagacgg cggagcttcc
     2281 ccatcgttcg cgagatgcgt gcgcagcagg agatcctcgc gttcggggcg gtacgtcacg
     2341 gtcgcgccga gaagctcggc tgccaggcgt cgcccatcgg cgggcccgat atccgcgtcg
     2401 tcgtcggcgt ccgtcgcgga gctgacgatc gcagcaggga acgtcaccca gcctgacacc
     2461 gagctaccgt cctgcctccg gctcaccgtg aggtccccga accccaggtc gggcccacga
     2521 tcgcgcacga agacgtcttc gcccgcgagt cccgggccgt tggtgtcgcc gggcacgagg
     2581 ttcgtcgcgc gactctgaaa cgcgacccag cgcccaccct cgctgacgcc gggcggcgag
     2641 accgaccagt cgtccggctg agctccagcc gaggagacgg atacgcgctc cgtgatgttc
     2701 gtctcccggt cgtggaggaa gacatcggag tggtcgttcg cgtctccctc gacgaggttg
     2761 gcagcggagc tgatgaagcc gacgtatcgc ccctcggggc tgatcctcgg cgacccagag
     2821 tcggcgtccc cttccactcc ctctgaggag acggagaccc gctcggtgat gttcttcgat
     2881 cggtcgcgca cgaagatgtc accgcaggcg ccggcgttcg ccgggagggg gacgcagttc
     2941 gtctggcc
//
LOCUS       DATLGG010000270         7694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_10197, whole genome shotgun sequence.
ACCESSION   DATLGG010000270 DATLGG010000000
VERSION     DATLGG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10197"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VM638_07705"
     CDS             complement(<1..1300)
                     /locus_tag="VM638_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HVM12343.1"
                     /translation="MPRSRFVALSCLLLLASLLSATPAQAATITVTKTADTNDGACNA
                     DCSLREALIRANTVAGADTIRLPAGTYVLTRLGKDDAAASGDLDVTSEVTMRPISGTV
                     QVDGNGIDRVIDVRSTGKLTVSRMRIVGGQPSGSVDGGGIRNAGTLDLIDSIVRGNAE
                     VREWGGGLVNTGTAKVTRSLISGNHGKYAGGVANFGILTVVDSRLTGNVSGDSGGGIY
                     NGSQITIIRSEVTGNWAEHGGGIYSTGQLTVERSRISRNMSSETGAGVRTGGPGVIRE
                     TVIEANRAGSDGGGLHVSGSDTFTVERSLLKANHAGSGGGIRNQGTLQLFDSTLARNR
                     ANDGGGLLGGGGTAYVIDVSIIDNLAGVDELDGNGGGINRQSGSVFLANTLVAGNVDL
                     VSPHHPDCIGAVISGGYNLLQTNGCSGYPGYDTDGTGAFSY"
     gene            complement(1406..1488)
                     /locus_tag="VM638_07710"
     tRNA            complement(1406..1488)
                     /locus_tag="VM638_07710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Leu,seq:taa)
     gene            1576..2064
                     /locus_tag="VM638_07715"
     CDS             1576..2064
                     /locus_tag="VM638_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVM12344.1"
                     /translation="MGVLPEPVEELLNVALVGEFTVIDAKGRPITHPMIPLYDGDKIY
                     LHSSILFSKKLEHIKGNPKVSLAITDTEATHGEPMRHRVTIQGDAVVYDRDPHRDWER
                     ILPLWIAKEPIIESFYKMRVALPLFWERALIEITPRRVLFWEGGRTDQPPQVFELEGV
                     GG"
     gene            2061..3434
                     /locus_tag="VM638_07720"
     CDS             2061..3434
                     /locus_tag="VM638_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HVM12345.1"
                     /translation="MSLVDELLRKCEEGYKHAVVAFVDRDGYPVSVATSYGVDPERGV
                     VKLRTPAGDLVPTEGTRVNVIVSHIRPAPEMGYDQRRYIQFWGEVTRSNGNLELKPRR
                     GHSWNEHEVPFFQYSEVSVRQGHEYLQRLSEEQGRPVKPRLSPLWLFLRATRLPFLSA
                     TVIPVLLGIAAAALHGEFHLGYGLLTLLAASFIHLALNVANDVFDTRSGADPANTTPT
                     QFSGGSRVIHYGLLSMKQMAWMSAAFYAAGIGIGVYLAIQRGVWPIVTLGAIGVAISV
                     FYTAPPFRLVHRGVGEVAVALGFGPIVLLGAYFVQAQQFSLEAAFLSAPVAILIALVL
                     YVNEIPDRRGDAQVGKRTLPVRWSKDAVITAYVWAAALTFMLIGGGAVAGVIARPAII
                     ALVTIPLAKKVVEGMRAHYDSPYELMGFMGTNIKLHAYTGGLLFAGYLIAIAADRILD
                     VAPVWLT"
     gene            3439..4848
                     /locus_tag="VM638_07725"
     CDS             3439..4848
                     /locus_tag="VM638_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVM12346.1"
                     /translation="MSGLGRYPLEPSTDETRRMLEEAARFVVAYLEGLEEAPAVNPDL
                     DPELRARIAEEPPAAGRPFAEVLHILERGAKEGFNTSGPGYLAYIPGGGIVAAGIADL
                     LADVMNRYVGIWQPAPAFAEIEWRVVRWLCDLFDLPAEARGILTSGGSMANFSAIVAA
                     RKAKLPEDFLNGTLYATDQAHHSVAKAAVLAGFPAESVRIVPHTPALRMDVGALREAI
                     AQDRAAGSRPFCVVASAGTTNTGAIDPIRDIVEVRDGEDLWLHVDGAYGGFFQLTERV
                     RRRFAGIERADSITLDPHKAMFLPYGTGALLVRDGERLRAAHSVHADYLQDLAGEAEV
                     PSFADHSPELSRDFRGLRVWLPLMLHGVEAFRAALEEKLDLTEMLHERLSAIPELEVP
                     WEPEVTVVTFRLRGDGDESTRALLDRINASRRVFLSSTLIDGRFFIRACIVSHRTHRD
                     RVEEAAEIIGAAVREVAGS"
     gene            4918..5925
                     /locus_tag="VM638_07730"
     CDS             4918..5925
                     /locus_tag="VM638_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HVM12347.1"
                     /translation="MPGSLRRLVAALVVAMIGGPVPVARANEALVVIAQIYGGGGNSG
                     APYTHDYVQLFNRGGAVADLEGWSVQYASATASTWAVTPLSGSLPPGGSYLIRLAAGS
                     GGAGAPLPGADAVGTTNLAASSGKVAVVRSAAPLPCGSSCASAVADLVGYGSANDFEG
                     SGPAPVLGNSLAAIREESGCRDTDDNRSDFLAGLPAPAGSSAAPAPCGGADPEDPPTD
                     PEDPPADPEACEPLTLEASVSPAVLWPPNRRMVAVSIRGIDGARLEEAVSSEADSVGP
                     GDPGDDIRVLAPTKVLLRAQRLGDGEGRRYTLRYSYEDGCGRTAKTEATVHVPHDRSP
                     A"
     gene            5992..>7694
                     /locus_tag="VM638_07735"
     CDS             5992..>7694
                     /locus_tag="VM638_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12348.1"
                     /translation="MRSSVTLVAAVLVGLPTVAEAAPYQRPGRTERVSVASDGSEGNA
                     DSSFTVPRLSSDGRFVAFGSAASNLVPGDANGVSDVFVRDRVRGTTERVSTAADGSEG
                     NASSGNPAISADGRLVAFHSNASNLVPGDTNGRQDIFVKDRETGEVRLVSVRPDGTPP
                     SAASSFPAISADGSRVAFHSDAADLVPGDTNGRRDVFVHDLKSGETVRASVTNDGGQG
                     TSDTRDIPDISGDGRFVVFTSGSQLTTEDTNNRLDVFVRDIETGRTELASISTDGKIQ
                     KENTAGSPSISADGRYVTFDSASWNMVPLDTNGDLGPIFDTFMMGFDVYVRDRKLGVT
                     EEVSVTPAGVEVDRDSGYSDISGDGRFVVFLSAGDLVTGERSPSGMYLHDRLTGVTER
                     VAVGIDGSPSTGGTPTISADGRSIAFQSSATNLVADDTNGKIDIFVRERGPSTGTLWL
                     EAEAEDGGIRARAALGLGGLTVSTAQDAAGDVPPGVAELGADLVGASLIHRAEQDDLL
                     LRLPVTAIPEVRDPRLDAGSSGTMGVVHGLDLTVDGVRFEVRASQRGAAISPRQMNPL
                     DG"
BASE COUNT         1163 a         2691 c         2664 g         1176 t
ORIGIN      
        1 tgtacgagaa cgccccggtg ccatcggtgt cgtagcccgg gtagccggag cacccgttcg
       61 tctgcagcag gttgtagcct ccggagatga ccgccccgat gcagtctgga tggtgtgggg
      121 aaacgaggtc gacgttcccc gcaacgagcg tgttcgcgag gaagacactg ccgctctggc
      181 gattgatacc gcccccattg ccgtcgagct cgtcgacccc ggcgaggttg tcgatgatcg
      241 aaacgtcgat gacgtaggcg gtcccgccgc cgcccaggag gccacctcca tcgttggccc
      301 tgttgcgcgc caacgtggag tcgaacagct gcagcgtgcc ctggttccgg atgccgcccc
      361 cactgccggc gtgattcgcc ttcagaaggc tccgttcgac ggtgaaggtg tccgagcccg
      421 agacatgaag cccccctccg tccgaaccgg ctcgattcgc ctcgatcacc gtctcgcgga
      481 tcactccggg accgccggtc ctgactcccg caccggtctc cgatgacatg ttcctgctga
      541 tgcggcttcg ttccaccgtg agctggcccg tcgaatagat cccgccgccg tgttccgccc
      601 agttcccggt gacctcgctc ctgatgatgg tgatctggct gccgttgtag atcccgcccc
      661 cgctgtcgcc gctaacgttc cccgtcagtc gactatcgac gaccgtgagg atgccgaagt
      721 tcgccacccc gcctgcgtac ttcccgtggt tcccactgat cagactccgg gtgacctttg
      781 ccgttccggt gttcacgagg ccgccgcccc actcccgaac ctccgcgtta cccctgacga
      841 tggagtcgat caaatccagg gtcccggcgt tgcggatacc gcctccatcc acgctgccgc
      901 taggctgacc tccgacgatc cgcatccgcg agaccgtcag tttccccgtg gagcggacgt
      961 cgatgacccg gtcgatgccg ttgccgtcga cctgcacggt gccggagatg ggcctcatgg
     1021 tgacctcgga ggtgacgtcg agatccccgg acgcagccgc atcgtccttc cccagccgtg
     1081 tcaagacata ggtccctgcg gggagtcgga tcgtgtccgc tcccgccacc gtgttcgccc
     1141 ggatgagggc ttcccgcagg gagcagtcgg cgttacaggc cccgtcgttc gtgtcggcgg
     1201 tcttcgtaac cgtgatcgtg gcggcctgag caggcgtggc ggagagcaag gatgccagca
     1261 ggagcaagca gctgagcgca acgaaccgcg aacggggcat gggcctccct ccctaggagg
     1321 gcgcagcgta gcaagaaacc gcgaaaccgg tcaatggaga cacgctctat cactcacggt
     1381 caccgaggtc gtgtgagaga tccggtgccc ggggtgggat tcgaacccac acggccctag
     1441 ggccagcgcc ttttaagggc gccgtgtctg ccgttccacc acccgggcgg gcccatcgta
     1501 gccgggcgag gcggtgcggg gtcggacgaa tcggctatcg ttcgatcgct ggaaccggtc
     1561 cctgaggagg cacccgtggg agtgttgccg gagcccgtgg aggagctcct gaacgtcgcc
     1621 ctcgtgggcg agttcacggt gatcgacgcg aaggggcggc cgatcacgca cccgatgatc
     1681 ccgctgtacg acggcgacaa gatctacctg cactcgagca tcctgttctc gaagaagctc
     1741 gagcacatca aggggaaccc gaaggtctcg ctcgcgatca ccgacaccga ggcgacccat
     1801 ggcgagccga tgcgccaccg cgtgacgatc cagggcgacg ccgtcgtcta cgaccgggac
     1861 ccgcaccgcg actgggagcg catcctcccc ttgtggatcg cgaaggagcc gatcatcgag
     1921 tccttctaca agatgcgcgt cgcgctgccg ctgttctggg aacgcgcctt gatcgagatc
     1981 acaccccggc gtgtcctctt ctgggagggc gggcgcaccg accagccacc gcaggtgttc
     2041 gagctcgagg gggtgggagg ttgagcctcg tcgacgagct cctgcgcaag tgcgaggagg
     2101 gctacaagca cgccgtcgtg gccttcgtcg accgtgacgg ctatccggtc agcgtcgcga
     2161 ccagctacgg cgttgacccc gagcggggcg tcgtgaagct ccgcacgccg gccggcgacc
     2221 tcgtcccgac cgagggcact cgcgtcaacg tcatcgtctc ccacatccgg ccggcgccgg
     2281 agatgggcta cgaccagcgc cggtacatcc agttctgggg cgaggtgacg cgctcgaacg
     2341 gcaacctcga gctgaagccg cggcgcggcc actcctggaa cgagcacgag gtgcccttct
     2401 tccagtacag cgaggtctcg gtgcggcagg gccacgagta cctccagcgc ctgtccgagg
     2461 agcagggtcg gccggtcaag ccgcggctca gccccctgtg gctgttcctg cgcgcgacgc
     2521 gcctgccgtt cctgtcggcg accgtcatcc cggtgctcct cggcatcgcg gccgccgccc
     2581 tgcacggtga gttccacctc gggtacgggc tgctcacgct cctcgccgcg agcttcatcc
     2641 acctcgccct gaacgtcgcg aacgacgtct tcgacacgag gagcggcgcc gacccggcga
     2701 acacgacccc gacgcagttc agcggtgggt cccgggtcat ccactacgga ctgctgagca
     2761 tgaagcagat ggcgtggatg tcggccgcct tctacgccgc ggggatcggg atcggggtct
     2821 acctcgcgat ccagcgcggg gtctggccga tcgtcacgct gggggcgatc ggggtcgcga
     2881 tcagcgtctt ctacacggcg ccgccgttcc gcctcgtgca ccggggcgtc ggcgaggtcg
     2941 cggtcgcgct cgggttcggc ccgatcgtgc tcctcggcgc gtacttcgtc caggcgcagc
     3001 agttcagcct cgaggcggca ttcctgtcgg cgccggtcgc gatcctcatc gcgctcgtcc
     3061 tgtacgtgaa cgagatcccg gaccgccgcg gcgacgcgca ggtcggcaag cgcacgcttc
     3121 cggtccggtg gtcgaaggac gcggtgatca ccgcgtacgt ctgggcggcg gcgctcacgt
     3181 tcatgctgat cggcggcggg gcggtcgccg gcgtgatcgc gcgaccggcg atcatcgcgc
     3241 tcgtgacgat cccactggcg aagaaggtcg tcgaggggat gcgcgcccac tacgacagcc
     3301 cctacgagct gatgggcttc atggggacga acatcaagct ccacgcgtac accggtgggc
     3361 tcctgttcgc cgggtacctg atcgcgatcg ccgccgaccg gatactcgac gtcgcgcccg
     3421 tctggctgac ctgagcgcat gagcgggctc ggccggtacc cgctcgagcc gtcgaccgac
     3481 gagacgcgac ggatgctcga ggaggccgcg cgcttcgtcg tcgcgtacct cgagggcctc
     3541 gaggaggcgc cggcggtgaa ccccgacctc gacccggagc tgcgcgcgcg catcgcggag
     3601 gaaccaccgg cggcgggacg tcccttcgcc gaggtcctcc acatcctcga gcgcggggcg
     3661 aaggaggggt tcaacacctc cgggcccggc tacctggcct acatccccgg cggcggcatc
     3721 gtggccgcag ggatcgcgga cctgctcgcc gacgtgatga accgctacgt cgggatctgg
     3781 cagccggccc ccgctttcgc ggagatcgag tggcgggtcg tgcgctggct gtgcgacctg
     3841 ttcgacctgc cggccgaggc gcgcggcatc ctcacgagcg gcggttcgat ggcgaacttc
     3901 tccgcgatcg tcgcggcgcg gaaggccaag ctccctgaag acttcctgaa cgggacgctc
     3961 tacgccaccg accaggcgca ccactcggtg gcgaaggcgg cggtgctcgc gggcttccca
     4021 gccgagagcg tgcgcatcgt gccgcacacg ccggcgctgc ggatggacgt cggggcgctg
     4081 cgcgaggcga tcgcgcagga tcgcgccgcc ggcagccggc cgttctgcgt cgtggcgagc
     4141 gccggcacga ccaacacggg cgcgatcgac ccgatccgcg acatcgtcga ggtgcgcgac
     4201 ggggaggatc tgtggctgca cgtcgacggg gcgtacggcg gcttcttcca actcaccgag
     4261 cgcgtgcggc ggcgcttcgc ggggatcgag cgggccgact cgatcacgct cgatccgcac
     4321 aaggcgatgt tcctcccgta cggcaccggg gccctgctcg tgcgcgacgg ggagcgactg
     4381 cgggcggccc acagcgtcca cgccgactac ctccaggacc tcgccggtga ggccgaggtc
     4441 ccgagcttcg ccgatcactc accggagctc tcgcgggact tccgcggcct gcgcgtgtgg
     4501 ctcccgctga tgctccacgg ggtcgaagcc ttccgcgcgg ccctcgagga gaagctcgac
     4561 ctcaccgaga tgctgcacga gcggctgtcg gcgatcccgg agctcgaggt cccctgggag
     4621 ccggaggtca cggtcgtcac cttccgcctg cgcggggatg gggacgagag cacccgggcg
     4681 ctgctggatc ggatcaacgc gtccagacgc gtcttcctgt cgagcacgtt gatcgacggg
     4741 cggttcttca tccgcgcgtg catcgtttcg caccgcacgc accgggaccg tgtcgaggag
     4801 gccgcggaga tcatcggcgc ggccgtccgg gaggtcgcag ggagctagcc cgagttcgtg
     4861 tcggtggccc gtgcgatgat cgggcccctc ctcctcacga ccggaaggag gaggaccatg
     4921 cccggaagtc tccggcggct cgtcgccgcc ctcgtcgtcg cgatgatcgg cggaccggtt
     4981 cccgtcgccc gcgcgaacga agccctcgtg gtgatcgcgc agatctacgg cggcggcggg
     5041 aacagcggcg cgccctacac gcacgactac gtccagctgt tcaaccgagg gggtgccgtc
     5101 gccgacctcg agggttggtc ggtgcagtac gcgtccgcga ccgcctccac ctgggccgtg
     5161 accccgctct cgggatcgct gccacccgga ggcagctacc tgatccggct ggccgcaggg
     5221 tccggcgggg cgggcgcccc gttgcccggt gccgacgccg tggggacgac gaacctcgcc
     5281 gcctcgagcg ggaaggtcgc ggtggtgcgc tcggcggcgc cgctcccctg cggatcctcg
     5341 tgcgcctccg cggtcgccga cctcgtcggc tacggaagcg cgaacgactt cgaaggctcg
     5401 ggcccggcgc cggtcctcgg gaactcgttg gcggcgatcc gtgaggaaag cgggtgccgg
     5461 gataccgacg acaaccggtc agacttcctc gccgggctcc ccgcccccgc cgggtcatcg
     5521 gcggcgcccg caccctgcgg cggagcggac ccggaggacc cgccgacgga tccggaggac
     5581 ccgccggcgg atccggaggc gtgcgagccg ctgacgctcg aggcgagcgt ctcgcccgcg
     5641 gtcctgtggc cgccgaaccg tcggatggtc gccgtgagca tcagggggat cgatggagcg
     5701 cgcctcgagg aggccgtctc ctccgaggcc gacagcgtgg gtccgggaga cccgggagac
     5761 gacatccggg tcctcgcccc aacgaaggtc ctgctccgag cgcagcggct cggcgacggc
     5821 gaagggcgtc gatacacgct ccgctattcc tacgaggacg gttgcggccg aaccgccaag
     5881 accgaggcga ccgtccacgt cccgcacgac cgcagtccgg catgagccgg gccggaaccc
     5941 ttgtgcacct gcacgaaggt atcgctgcgg gcggtgtcga accgagagcg ggtgagatcc
     6001 tccgtcacgc tcgtcgccgc cgtgctcgtc ggtctcccga cggtcgctga ggccgccccg
     6061 taccagcgcc cgggtcgcac ggagcgtgta tcggtggcct cggacggctc ggaggggaac
     6121 gcagactcgt ccttcacggt gccgcggctt tcctcggacg gacggttcgt cgccttcggg
     6181 agcgccgcgt ccaacctggt gcccggggac gcgaacggtg ttagcgacgt attcgtgagg
     6241 gaccgggtcc ggggaaccac ggaacgtgtc tcgacggctg ccgacggctc ggaggggaac
     6301 gcgtcatccg ggaaccctgc tatcagcgcc gacggacgcc tcgtggcgtt ccacagcaac
     6361 gcctccaacc tggtacccgg tgacaccaac gggcggcagg acatcttcgt gaaggacagg
     6421 gagacagggg aggtgcggct cgtctccgtg cgccccgacg gcacgccgcc cagcgccgcg
     6481 tcgagcttcc cggcgatctc ggccgacggg tcgcgggtgg cgttccacag cgacgcggcg
     6541 gacctcgtgc ccggcgacac gaacggccgg cgagacgtct tcgtgcacga cctgaagagc
     6601 ggggagaccg ttcgagcctc ggttaccaac gacggcgggc aggggacctc cgacacgcgc
     6661 gacatccccg atatctccgg agacggacgg ttcgtggtct tcacgagcgg gtctcagctc
     6721 acgacggagg acacgaacaa ccgcctcgac gtcttcgtcc gcgacatcga gacgggccgc
     6781 accgagctgg cgtccatctc gacggacggc aagatccaga aggagaacac cgcagggagc
     6841 ccgagcatca gcgccgatgg ccggtacgtc accttcgact cggcttcatg gaacatggtg
     6901 ccgctcgaca cgaacgggga cctcggcccg atcttcgaca cgttcatgat gggcttcgac
     6961 gtctacgtcc gagaccgcaa gctcggcgtc accgaggagg tctcggtcac tccggcgggg
     7021 gtggaggtcg accgggactc ggggtactcg gacatcagcg gcgacggacg gttcgtcgtc
     7081 ttcctcagcg ccggcgatct cgtcacgggc gagcgctcgc cgtcggggat gtacctccac
     7141 gaccggttga ccggggtaac cgagcgcgtg gcggtcggga tcgacggctc accctccacc
     7201 ggaggcacgc cgacgatcag cgccgacgga cgatcgatcg ccttccaaag cagcgccacg
     7261 aacctcgtcg ccgacgatac gaacggcaag atcgacatct tcgtgcggga acgcggcccc
     7321 tcgacaggca cgctatggct ggaagcggag gcggaggacg gcgggatccg agcgagggcc
     7381 gccctcgggc tcgggggcct cacggtctcg accgcgcagg atgcagcagg cgacgtcccg
     7441 ccgggcgtcg ccgagctggg tgcggatctg gtcggcgcct ccctgatcca tcgggcggag
     7501 caggacgacc ttctcctgcg gctgcccgtc accgcgatcc ccgaggtccg agacccgcgc
     7561 ctcgacgccg gcagctccgg gacgatgggg gtcgttcacg gcctcgacct caccgttgac
     7621 ggcgtccgct tcgaggtccg cgcctcccag cgcggcgctg cgatctcccc gcggcagatg
     7681 aaccctctcg acgg
//
LOCUS       DATLGG010000271         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_172993, whole genome shotgun sequence.
ACCESSION   DATLGG010000271 DATLGG010000000
VERSION     DATLGG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172993"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VM638_07740"
     CDS             <1..1151
                     /locus_tag="VM638_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12349.1"
                     /translation="FGIWAGASAGTTILGPFVGGLLVNTVSWRAAFLLNVPLLLVALW
                     ATLRHVPESRDEAAPARFDWTGSVFVILAVGGLAFGTIRGQEHGWAAPAAAASLGVGA
                     GALLVLPLLMRHRRDALVPPELFRSRNFRVTNLSTFLIYGALYVSLTFQALFLVGTLG
                     YSEPAAGLATLPSTLLLALFSTRFGRLAARIGPRRFMAAGPAVMGVGLLLLTRVPATS
                     EPWRLGAGQGWSLMPPTDYLVHFLPAILVFGAGLMVMVAPLTTAVMTSVPSRNAGLAS
                     AINNAVSRVGSPLVTAVLFVAVVASFHGSIERRLPPAGPASETVRERVAPLNVATAPP
                     AARPAAREASTDAFHLAMAAAAALLFAGGAINLRIDDRQALRGSDDTA"
     gene            complement(1148..2092)
                     /locus_tag="VM638_07745"
     CDS             complement(1148..2092)
                     /locus_tag="VM638_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HVM12350.1"
                     /translation="MRIVRRAVLGAAALALLLLGAACGGQPGGGGNGDGRTVASTLIL
                     GGPPECPERPFCIPGLRETYGIEFADFVPLDVGGPLTVQALEADEIDVALLFSTSSVI
                     GEKNWVVLEDDENLQNAENITPVVRREAAQPALTERLNAVSRALTTEEITALNARVEV
                     SGDDPADVAAEFLAREGLLDGPTGSGAVTVGAVSFAENQIVAEMYAQTLEAAGYDVER
                     QLALESREIVQPALAAGDIDVAPEYLSSLLLFVQADAEASGVPGAVRQKLEPILAERG
                     LLLLESSKANDTNAFVVTRATAEEYGLVRMSDLAKPLP"
     gene            complement(2172..>2526)
                     /locus_tag="VM638_07750"
     CDS             complement(2172..>2526)
                     /locus_tag="VM638_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVM12351.1"
                     /translation="ATVEAARGMGMGERRLLLGVELPLALPLILAGLRTASLQVVATA
                     SLAAVVAGGGLGRFIIDGFALGESGRPQVFAGALLVALLALLTEGAFALTERVVGRAR
                     ATRRASGPLPPPLGA"
BASE COUNT          302 a          924 c          906 g          394 t
ORIGIN      
        1 ccttcgggat ctgggccggt gcatcggcgg ggacgacgat cctgggccct ttcgtgggtg
       61 gccttctggt gaacacggtc tcgtggcgag ccgcgttcct gctgaacgtg ccgctgctcc
      121 tggtggccct gtgggcgacg ctgcgccacg tccccgagag ccgtgacgag gccgctcccg
      181 cccgcttcga ctggacgggg tcggtcttcg tgatcctggc ggtgggcggc ctcgcgttcg
      241 gaacgatccg cggtcaggag cacgggtggg cggcgcccgc ggccgccgcg agcctcgggg
      301 tcggagccgg ggcgctgctc gtgctgccgc tgctcatgcg gcatcgtcgc gatgcgctcg
      361 tcccgcccga gctgttccgc tcccggaact tccgggttac gaacctttcg acgttcctga
      421 tctacggcgc gctctacgtc tcgcttacgt tccaggcgct cttcctcgtg gggaccttgg
      481 ggtattcgga gccggcagcg gggctggcga ccctgccatc cacgctgctc ctggcgctct
      541 tctcgacgag gttcgggcgg ctcgcggctc ggatcggacc tcgtcggttc atggcggcgg
      601 gtccggcggt gatgggggtc ggtctcctgc tcctgacccg cgtgccggcg accagcgagc
      661 cgtggcgact cggcgccggg caggggtggt cgctcatgcc tcccaccgac tacctcgtgc
      721 acttcctgcc ggcgatcctc gtcttcgggg ccgggttgat ggtcatggtg gcgccgctga
      781 cgacggccgt catgacatcg gtcccgagtc gcaacgcagg tctcgcgtcg gcgatcaaca
      841 acgccgtgtc acgggtgggc tccccgctcg tgaccgcggt cttgttcgtg gccgtcgtcg
      901 cgtcgttcca tgggtcgatc gagcgccgcc tcccgccggc ggggccggct tccgagaccg
      961 tccgcgagcg ggtcgcgccg ctgaacgtcg cgacggctcc acctgccgcc cgtccggctg
     1021 ccagggaggc ctccaccgac gccttccatc tcgcgatggc ggccgccgcc gccctgctgt
     1081 tcgccggtgg ggcgatcaac cttcggatcg acgaccgtca ggctctgcgg ggctccgacg
     1141 acacggccta ggggagcggc ttcgcgaggt cgctcatccg cacgagcccg tactcctctg
     1201 ccgtcgctct ggtgaccacg aaggcgttcg tgtcgttcgc cttcgacgac tcgagcagca
     1261 ggagtccgcg ttcggccagg atcggttcga gcttctgccg gacggcgccc gggacgccgg
     1321 aggcctcggc atcggcttgc acgaacagca ggagcgagct caggtactcc ggcgccacgt
     1381 cgatgtcgcc ggccgcgagc gccggctgga cgatctcacg cgactcgagc gccagctggc
     1441 gctcgacgtc gtagccggcg gcctccagcg tctgggcgta catctccgcg acgatctggt
     1501 tctcggcgaa cgagaccgcg ccgaccgtca cggcgccgct cccggtcggc ccgtcgagga
     1561 ggccctcccg cgcgaggaac tcggccgcaa cgtcggcggg atcgtcgccg gagacctcca
     1621 cccgcgcgtt cagcgcggtg atctcctcgg tcgtcagggc gcggctcacg gcgttcagcc
     1681 gttcggtgag agccggttgg gcggcctccc ggcggaccac cggcgtgatg ttctcggcgt
     1741 tctgcaggtt ctcgtcgtcc tcgaggacga cccagttctt ctcgccgatc acgctcgacg
     1801 tcgagaacag cagcgcgacg tcgatctcat cggcttcgag ggcctggacc gtcaacgggc
     1861 cgccgacgtc gagcgggacg aagtcggcga actcgatccc gtaggtctcg cgcagcccgg
     1921 ggatgcagaa gggccgctcc gggcattcgg gagggccacc gaggatcaag gtgctcgcga
     1981 cggtgcgtcc gtcgccgttc ccgcccccac ccggctgccc gccgcatgcc gctccgagga
     2041 gcagcagcgc cagcgcagcc gccccgagga cggctctgcg gacgatccgc atgggtctgc
     2101 ctcctttccg ggagccagat gcggcccctt cggcggccca acctacagga gcgcggagtg
     2161 agccgcgggc ctcaggcgcc gagcgggggc gggagcggcc ccgacgcgcg ccgggtcgcc
     2221 cgtgcccggc ccaccacgcg ctccgtcagg gcgaaggcgc cctccgtgag gagggcgagg
     2281 agggcgacga gcagcgcccc cgcgaagacc tggggccggc cgctctcacc gagggcgaac
     2341 ccgtcgatga tgaaccgccc gagcccgccc ccggcgacga cggccgcgag gctcgcggtc
     2401 gcgaccacct gcagcgaggc cgtgcgcagc ccggcgagga tcagcggcag ggccaggggg
     2461 agctcgaccc ccaggagcag gcgccgttcg cccatcccca tcccgcgggc ggcctcgacc
     2521 gtcgcc
//
LOCUS       DATLGG010000272         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_104634, whole genome shotgun sequence.
ACCESSION   DATLGG010000272 DATLGG010000000
VERSION     DATLGG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104634"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VM638_07755"
     CDS             <1..825
                     /locus_tag="VM638_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12352.1"
                     /translation="AGGDLVWSVHNVLPHDTERPELEAALQQGIVDRATIIHVLSERT
                     PDIAREWFTIPPEKVLHVPHQSYAGAYLETVSREDARWQLGIGPDEVVYALLGAIKPY
                     KGTDRLLDAFDVLSSGDPGRRRLIVAGMPDQGGAVDRVLARCERHPFVLLHATTIPAD
                     DMQLYLRAADIVVLPYLRSLNSGVLMLALTFGVPVVAPAAGSIPEILTPEVGRTFDPE
                     SADSLLEALTAADALRSPEARAAARRRAADYDAERLSHEFGRGIRERVAASLPTWA"
     gene            899..1747
                     /locus_tag="VM638_07760"
     CDS             899..1747
                     /locus_tag="VM638_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM12353.1"
                     /translation="MTSTPSEAFASPRVSAPRRGPVAQAMQGLREIIERRRLIRYLVG
                     ADLKRTHADTIFGQVWWILDPLLQMAIYVVLVTVIFNRPIPDYPLFLFAGILPWKWFA
                     TTLSDSAISITGRQSLIRQVQFPKVVLPTASVTAGSVSFVIGLVALAFVYLFFLHRLT
                     PWVLLIPVIAVVQYVFTLALAIALSALNAFFRDVQNVLRHTLRLWFYMSAILYPLDRV
                     PEGLRPILLLNPFTHLVDAYRSVTWGTTAPDWLSLLVVLVLSLVLVVLALGLFKRLEP
                     AFARIL"
     gene            1828..2691
                     /locus_tag="VM638_07765"
     CDS             1828..2691
                     /locus_tag="VM638_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12354.1"
                     /translation="MAAPTLPVGAPTAAAEPDARAGDPAIDVRDLGIRYNLRLTRRTT
                     LKGSLPAVLQRRRDDDAPRHFWALRHVSFRLEHGESLAIIGPNGAGKSTLLLALAGIL
                     EPSEGAIRTYGRVSTLLTLGAGFEQELTGRDNIDLVAAFLGIDHREMAALTPSIVEFA
                     DIGPFIDAPVKTYSTGMKARLGFAIATAITPDILLLDEVLGTGDQTFRARSQARVREL
                     VDRAKAIVLVTHDLTSVTEFCTRAILVERGQIIQEGAPGEVVKLYRERVAERKLMAEA
                     EAERFARIPGS"
BASE COUNT          382 a         1029 c          921 g          458 t
ORIGIN      
        1 gccggcgggg acctcgtctg gtcggttcac aacgttctgc cgcacgatac ggagcggccg
       61 gagctcgagg cagcgctcca gcagggcatc gtcgaccgcg cgacgatcat ccacgtcctg
      121 tccgagcgaa cccccgacat cgcccgggag tggttcacga ttcctcccga gaaggtgctc
      181 cacgtcccgc accagagcta tgcgggtgcc tatctcgaga cggtgagccg ggaagacgcc
      241 cgctggcagc tcgggatcgg gccggacgag gtcgtctacg cgctgctcgg ggcgatcaag
      301 ccatacaagg gaacggaccg cctgctcgac gcgttcgacg tcctgtcgag cggcgacccc
      361 gggcggcgcc ggctgatcgt cgccggcatg ccggaccagg gaggcgccgt cgaccgcgtg
      421 ctcgcccgct gcgagcgaca tcccttcgtg ttgctccacg cgacgaccat cccggcggac
      481 gacatgcagc tctacctgcg ggcagctgac atcgtggtcc tgccctacct gcgctcgctg
      541 aattcgggcg tcctgatgct cgccctcacg ttcggcgtcc cggtggtggc ccccgcggca
      601 ggcagcatcc cggagatcct gacacccgag gtcgggcgaa ccttcgaccc cgagtccgcc
      661 gactcgctgc tggaggcgct caccgccgcg gacgcgctgc gctccccgga ggcccgcgcc
      721 gccgcgcgca gacgggcggc cgactatgac gccgagcgcc tgtcgcacga attcgggcgc
      781 gggatccgcg aacgcgtcgc ggcatcgctc ccgacgtggg cctgagcctg agccgccgga
      841 atcgtagcgg tcgatgcccg gggaacccgg gtcaccccgt gctaacctga cgcggcccat
      901 gacctcgacc ccgtcggagg cgttcgcctc accgcgcgta tcggcccctc gccgaggccc
      961 ggtcgcccag gcgatgcagg gcctgcggga gatcatcgag cggcggcggc tgatccgcta
     1021 tctcgtcggc gcagacctca aacggacgca cgcggacacg atcttcgggc aggtctggtg
     1081 gatcctcgac cccctcctcc agatggccat ctacgtggtc ctcgtcacgg tcatcttcaa
     1141 ccgcccgatc ccggactacc cgctcttcct gttcgcgggg atcctgccct ggaagtggtt
     1201 cgccacgacg ctgagcgatt cggcgatctc gatcaccggc cggcagagcc tcatccggca
     1261 ggtccagttt ccgaaggtcg tcctgccgac cgcttcggtc acagccgggt cggtgagctt
     1321 tgtcatcggg ctcgttgcgc tcgcgttcgt ctacctcttc ttcctgcacc ggctcacgcc
     1381 gtgggtcctg ctcatcccgg tcatcgcggt cgttcagtac gtcttcacgc tggcgctcgc
     1441 gatcgccctg tccgcgctca acgcgttctt ccgggacgtc cagaacgtcc tgcgccacac
     1501 cctccgcctg tggttctaca tgtccgcgat cctgtacccg ctcgatcgcg ttccggaggg
     1561 tctgcggccc atcctcctgc tcaacccgtt cacgcatctc gtggatgcgt accggtcggt
     1621 gacctggggg acgaccgcgc ccgactggct ctcgctgctg gtcgtcctcg tgctctccct
     1681 tgtcctcgtg gtcctggcgc tcggcctgtt caagcgcctc gagccagcgt tcgcgcggat
     1741 cctgtgacgg ccgatccgac caccacgccg ccgacaccgg gcgagggtgg ccgggagttt
     1801 ccccccgcca cgcgaagcga tgccgacgtg gcggccccga cccttccggt cggcgcgccg
     1861 actgcggccg cggagccgga tgcgcgggcg ggagatccgg ccatcgatgt ccgcgacctc
     1921 gggatccggt acaacctccg actcacgcgc cggaccaccc tgaagggctc cctgccggcg
     1981 gtcctccagc gccgccgcga cgacgacgcg ccgcgccatt tctgggccct ccgccacgtg
     2041 agctttcgcc tcgagcacgg cgagtcgctc gccatcatcg gccccaacgg tgccgggaag
     2101 tccacgctcc tgctcgccct ggccggcatc ctcgagccgt cggagggagc gatccggacc
     2161 tacggtcggg tgtccaccct gctgaccctg ggcgccggct tcgagcagga gctgaccggc
     2221 cgggacaaca tcgacctcgt ggccgccttc ctgggcatcg accatcgcga gatggcggcg
     2281 ctcacgccgt cgatcgtcga gtttgccgac atcgggccgt tcatcgatgc cccggtcaag
     2341 acgtactcca cggggatgaa ggcgcggctc gggttcgcca tcgcgaccgc catcacgccg
     2401 gacatcctgc tcctcgacga ggtcctgggg acgggcgacc agacgttccg ggcccggtcg
     2461 caggctcgtg tgcgtgagct ggtcgatcgt gccaaggcga tcgtgctcgt cacgcacgac
     2521 ctcacctcgg tgacggagtt ctgcacgcgg gcgatcctcg tggagcgcgg gcagatcatc
     2581 caggagggag cacccggcga ggtggtgaag ctctatcgcg agcgcgtcgc cgagcgcaag
     2641 ctcatggcgg aggcggaggc ggagcggttc gcccgcatcc ccggcagctg aggcggccaa
     2701 gggaggttcg atgacccgtc cccacggcca gggatggcgg acgacggtcc gccggatgat
     2761 cgaccgcctt ccggtcgggc gaggtctcgt
//
LOCUS       DATLGG010000273         9583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_173083, whole genome shotgun sequence.
ACCESSION   DATLGG010000273 DATLGG010000000
VERSION     DATLGG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173083"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VM638_07770"
     CDS             complement(<1..462)
                     /locus_tag="VM638_07770"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVM12355.1"
                     /translation="MTWHPHVDVWILVTALSAGYAYALRRIGPRQVPAGLPVASRGQV
                     TAFSFGVAAIWIAGDWPVHDLAEGSLYSVHMVQHMLITLVAPPLLLMGTPAWLARRLL
                     RPAWVLRWVRRLSRPLVALLLFTAVVAVSHWRVAVELSVRSELFHFGAHAVL"
     gene            complement(459..794)
                     /locus_tag="VM638_07775"
     CDS             complement(459..794)
                     /locus_tag="VM638_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HVM12356.1"
                     /translation="MTEAEAHVEDRTHVETHEASHPSVGEYIKIAIILAIVTGLEVGI
                     AYIPGIPFGWLVASLIVLMVIKFAMVAMWFMHLKFDSAIYRRFFVIGLVLAVVLFLIV
                     LVSFGKFLR"
     gene            complement(791..1396)
                     /locus_tag="VM638_07780"
     CDS             complement(791..1396)
                     /locus_tag="VM638_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVM12357.1"
                     /translation="MATSDAHLGSADHPATATGLDNRKLAMWAFLGSECLFFGALIST
                     FLLYRERVPEGGVRPEDVYDIPFTSVSSFVLLMSSLTMVLALAAIQRGDHRQLRIWLV
                     ATAGLGMVFIAGQIFEFTAFIEHGFTMRSSVSGSAFFVLTGFHGAHVTVGILMLLVMA
                     MFSAAGRLTQEHTMRIEMLGLYWHFVDIVWVVIFTLVYLIP"
     gene            complement(1397..3304)
                     /gene="ctaD"
                     /locus_tag="VM638_07785"
     CDS             complement(1397..3304)
                     /gene="ctaD"
                     /locus_tag="VM638_07785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HVM12358.1"
                     /translation="MAVVAAPTRRPWPRRPSATTGFSSWFTTVDHKKIGILYGVTAFF
                     FFLVGGVEALLIRINLAAPTTPEGALIDADTYNQIFTMHGVTMVFFVVMPLGAAFFNY
                     FIPLMIGARDVAFPRLNAFSYWVFLLGGLFVYSGIFLGGLPDGGWFAYAPLSSMEPGA
                     RMTFYAIGLQIAGVASLVAAVNFIVTILNLRAPGMSLMRMPVFVWMTLVVQFLLLFSL
                     PILAIGLFQLTFDRAFSSLFFNPTAGGDPVLWQHLFWLFGHPEVYILILPAMGIVSEV
                     LPVFSRKPLFGYAAIVFAGVAIGFIGFGVWAHHMFAVGLGAIANTAFGVSTMIIAVPT
                     GIKIFNWLGTMWRGALTLTTPMLFAIGFVTMFVIGGLSGVTHAVVPADYQQTDTYYVV
                     AHFHYVLFGGAIFGLFSGIYYWWPKFSGRMFNERLGKLHFWIMLIGFNLTFGPMHILG
                     LQGMPRRQYFFSEGLGWDLWNMVSTIGAFTIAVSVLVFMFNAVRSLAAGERATADPWD
                     ARTLEWTIPSPPPPHNFDEIPTVHARDELWHRKYSEDRQGRPIPVVAGAAGEAAHGEA
                     HGEAHDDGTGDEHAGQHGFHLPLPSYWPLVAAIGLPLIGYGLMFHWVFLAAGTATLLA
                     GLYGWMLEPLE"
     gene            complement(3317..4420)
                     /gene="coxB"
                     /locus_tag="VM638_07790"
     CDS             complement(3317..4420)
                     /gene="coxB"
                     /locus_tag="VM638_07790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVM12359.1"
                     /translation="MTSRRMRSPLRPLLPLVVPLALLLAACRDDLPASTLNPAGPQAR
                     EIDNLFRPVFWIAVGVFVVVEALIVFAVFRFRRRPGDAANPVQVHGNTRLEIIWTAIP
                     AIILAVIAVPTVRTIFALAETPEDPNLVNVRVIGHQWWWEFQYPELGIVTANEIHLPV
                     DQPVHLEMTSEEPAASGVDKNAIPVAHSFWIPRLAGKQDVLPGRTTRMTIQAEDPGIY
                     LGQCAEFCGLSHANMRLRAIAHTEQDFDAWVRSMRSPAPEPAEGTLEAEGAELFQRFV
                     TPEYPDGFSCLACHTVDGTVGDQVGPNLARYGERETLGAGVFDNTEANLRRWLRDPRA
                     MKPGVVMPDYGLSEEQIDALVAYLMSLGAEDGQ"
     gene            complement(4448..4678)
                     /locus_tag="VM638_07795"
     CDS             complement(4448..4678)
                     /locus_tag="VM638_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12360.1"
                     /translation="MSPPLAFLIDLIRRHGWHAIGELAALLLAAVFTFFFVRALIRGQ
                     VRAIVCGSCGRVASRADPRCPRCGAALDRASM"
     gene            complement(4675..6447)
                     /locus_tag="VM638_07800"
     CDS             complement(4675..6447)
                     /locus_tag="VM638_07800"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVM12361.1"
                     /translation="MTLLRRTSTITAVAALALVAVGGVVRATGSGLGCPDWPRCHGRL
                     IPPFEFHALIEYSHRLTAAIVGVLVLLTAWFAWRRARGDRAIVWPAAVAVLVVGIQAA
                     LGRMVVVGELQVVTMNMAHFLTAMLLVALVVIVAAASWIPRGSGVAVDRDVRGLLWWT
                     LGITAVLLLAGIYVRATGASLAFLDWPLMEGRFLPALDERGETAHFVHRALALIAVGF
                     GAAFAWRARRSGNVALRRLAWGAFALLLVQALVGAAAVLTRLATPAVAAHVALSSLAW
                     ATLVTATVVATRAGRGGVARQERPAARSVAGAYVQLMKPDIIVLLLITTVPAMVLAAG
                     GIPSGALVGATLLGGALAAGGAGAINGYLDRDIDEVMVRTRRRPIPAHRVSPGGALRF
                     GIALVLAGTIWLAVTVNLPAAALTFAAAAFYVVVYTIWMKRSTPQNIVIGGAAGAAPA
                     LIGWAAVTGTVELPAVVLFAIVFVWTPPHFWALSLRHEAEYEAARIPMLPVVRGREAT
                     KRQILVYSLILLATSLLLAPVAALGAVYLATAIALGAAFVAQAVRLRRPGAGNREAMA
                     LFHFSITYLAVLFAAAAVDRLVLA"
     gene            6500..6955
                     /locus_tag="VM638_07805"
     CDS             6500..6955
                     /locus_tag="VM638_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12362.1"
                     /translation="MPRPRSVVTIALALAFVSAACGQKAGVQRDGRVGGDPGPTEPAP
                     IAPLPATDAAGAVMALCAAEAAAATGDVEGMRSAFEDRAHAFLHQFADTLQATDPAIA
                     AGLLEAKQQVEAGLADGTPPPEMAARLAALRSQVEQGAAANGTPTQGCA"
     gene            6952..7893
                     /locus_tag="VM638_07810"
     CDS             6952..7893
                     /locus_tag="VM638_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12363.1"
                     /translation="MRRALALSLAVLAGACGGGAEDPPADRRGGGLVAQVASYEPLAG
                     EDNRLIVGLLTEDNLFVSGGTATFRFSYLGEEGAAPVPGPEVQAGFLPIAEPGAVPPP
                     EQPTVGGATDVGRGVYAARGVRFDRAGLWEVEVIATIDGEERRATSAFTVLEEPLVPG
                     VGERAPRTKNLTIHDGDAPPQAIDSRAETEGEVPDPDLHDTTIAAAIRRGRPVLAVFA
                     TPVYCVSRFCGPITDMVAGLRERWGDRVEIIHVEIWRDFQEQVVNKGAAEWVLREDNL
                     TEPWIFLIDGDGRIAARWDNVATPGEIEPELERVTGG"
     gene            7901..8992
                     /locus_tag="VM638_07815"
     CDS             7901..8992
                     /locus_tag="VM638_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVM12364.1"
                     /translation="MTTLAVEPFQLPPRCVELAGRSEDALRGFLEDQRTAIVGPDPGA
                     SAAFDELERVVWAGGKRLRPVFCCLGYLATGAEEDERLFAAAAALELLHTFALVHDDV
                     MDRSPTRRGTPTTWIHLAERHRREGWRGDPVAYGISAAVLVGDLAIALADQAFMRSGF
                     ASDRLLPGAVRYHRMRTDMVAGQFLDVEASQRTGISEAAARRIAVLKSGAYTVEGPLQ
                     IGASLAGSADGLLDALSRYGTALGEAFQLRDDLLGSFGDPSVTGKDRDSDLREGKRTV
                     PVALALAAAPEADRRFLEERLGHADLSDDEVERMRGIVERSGAVAATIGLVEDLSEEA
                     RRSAAEAPVPPEVASLLVSMVDALAARPM"
     gene            9040..9504
                     /locus_tag="VM638_07820"
     CDS             9040..9504
                     /locus_tag="VM638_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVM12365.1"
                     /translation="MNLLDARRVFAPLHVTQVATTRVDGSPHVVPLWFVWREDAIYVS
                     CRRDSVTWRAVERDRRVALSFHVGEAWSELAGAVVYGRADPIAADHPALRGVMSAWFE
                     KYRSLLAGAAFAHYAREVEAPGMLRVRPHRLISWDHAIAARRASQHDEAADA"
BASE COUNT         1537 a         3338 c         3504 g         1204 t
ORIGIN      
        1 caggaccgcg tgcgcgccga agtggaagag ctccgaacgc accgagagct cgaccgccac
       61 ccgccagtgg gagaccgcaa cgacggccgt gaacagcagg agcgcgacga gcggcctgga
      121 cagccgccgc acccaccgca gcacccacgc gggccgcagc agccgccggg cgagccacgc
      181 gggcgtcccc atcagcagga gcgggggcgc gacgagcgtg atcagcatgt gctggaccat
      241 gtggacgctg tacagggacc cctcggcgag gtcgtgcacc ggccagtcgc cggcgatcca
      301 gatcgccgcg accccgaacg agaacgccgt gacctggccg cggctcgcga ccgggaggcc
      361 ggccggcacc tgccgcggcc cgatgcgccg gagagcatac gcgtagcccg ccgagagcgc
      421 ggtcacgagg atccagacgt cgacgtgggg gtgccaggtc atcgcaggaa cttcccgaag
      481 ctcacgagga cgatcaggaa caggacgacg gcgaggacga gcccgatcac gaagaaccgg
      541 cggtagatcg cgctgtcgaa cttcaggtgc atgaaccaca tcgcgaccat cgcgaacttg
      601 atgaccatga ggacgatgag cgaggcgacg agccatccga aggggatccc cgggatgtag
      661 gcgatcccga cctccaggcc ggtcacgatc gcgaggatga tcgcgatctt gatgtattcg
      721 ccgacgctcg ggtgcgacgc ctcgtgcgtc tcgacgtgcg ttcgatcctc cacgtgcgcc
      781 tccgcctcgg tcatgggatc aggtagacga gggtgaagat cacgacccag acgatgtcga
      841 cgaagtgcca gtagaggccg agcatctcga tgcgcatcgt gtgctcctgc gtgagccggc
      901 ccgccgccga gaacatcgcc atcacgagca gcatgaggat cccgaccgtc acgtgcgcgc
      961 cgtggaaccc ggtcagcacg aagaaggcgc ttcccgagac cgacgagcgc atcgtgaagc
     1021 cgtgctcgat gaaggccgtg aactcgaaga tctgcccggc gatgaacacc atcccgagtc
     1081 ccgccgtcgc gacgagccag atccgcagct gccggtggtc gccgcgctgg atcgcggcga
     1141 gggcgagcac catcgtgagc gatgacatga gcaacacgaa cgagctcacc gacgtgaacg
     1201 ggatgtcgta gacgtcctcg ggacggacgc cgccttccgg aacacgctcg cggtagagga
     1261 ggaaggtcga gatcagggcg ccgaagaaca ggcactccga cccgaggaag gcccacatcg
     1321 cgagcttccg gttgtcgagg ccggtcgcgg tcgccgggtg gtcggcgctg ccgaggtgcg
     1381 cgtcggacgt cgccatctac tcgagcggct ccagcatcca gccgtagagg ccggcgagca
     1441 gggttgcggt gccggccgcg aggaacaccc agtggaacat cagcccgtag ccgatcagcg
     1501 gcaggccgat cgcggccacc aggggccagt acgagggcag cgggaggtgg aacccgtgct
     1561 ggcccgcgtg ctcgtccccg gttccgtcgt cgtgcgcctc accgtgcgcc tcgccgtgcg
     1621 cggcctctcc tgcggctccg gccacgacgg ggatcggccg tccctgccgg tcttcggagt
     1681 acttgcgatg ccacagctcg tcgcgggcgt gcacggtcgg gatctcgtcg aagttgtgcg
     1741 gaggcggcgg cgaggggatc gtccactcga gcgtccgcgc gtcccagggg tcggcggtgg
     1801 cgcgctcgcc cgccgcgagg ctccgcacgg cgttgaacat gaacacgagg accgagaccg
     1861 cgatcgtgaa cgccccgatc gtcgaaacca tgttccacag gtcccagccg agcccttcgg
     1921 agaagaagta ctggcgccgg ggcatcccct gaagcccgag gatgtgcatc ggtccgaacg
     1981 tcaggttgaa cccgatcagc atgatccaga agtgcagctt gccgaggcgc tcgttgaaca
     2041 tgcgcccgct gaacttcggc caccagtagt agatgccgga gaagaggccg aagatcgctc
     2101 cgccgaacag cacgtagtgg aagtgggcga cgacgtagta cgtgtcggtc tgctggtagt
     2161 cggcggggac gaccgcgtgc gtgacgccgg agaggccgcc gatcacgaac atcgtcacga
     2221 agccgatcgc gaagagcatc ggcgtcgtca gcgtgagcgc gcctcgccac atcgttccga
     2281 gccagttgaa gatcttgatg ccggtcggca ccgcgatgat catcgtggag acaccgaacg
     2341 cggtgttcgc gatcgccccg agacccacgg cgaacatgtg gtgcgcccac acgccgaacc
     2401 cgatgaagcc gatcgcgacc ccggcgaaga cgatcgccgc gtagccgaac agcggcttgc
     2461 gggagaacac cggcaggacc tcggagacga tccccatcgc cgggaggatc aggatgtaga
     2521 cctcgggatg gccgaagagc cagaacaggt gctgccacag gaccgggtca ccgccggcgg
     2581 tcggattgaa gaacagcgat gagaaggcgc gatcgaacgt gagctggaag agcccgatcg
     2641 ctaggatcgg gagggagaag agcagcagga actgcacgac gagcgtcatc cagacgaaca
     2701 cgggcatccg catcaggctc atcccgggtg cccggaggtt caggatcgtg acgatgaagt
     2761 tcacggccgc gaccaacgag gccacacccg cgatctgcag gccgatcgcg tagaacgtca
     2821 tgcgcgcgcc cggctccatc gagctgaggg gcgcgtaggc gaaccagccg ccgtcgggta
     2881 gacccccgag gaagatgccg gagtagacga agagaccgcc gagcaggaag acccagtagc
     2941 tgaacgcgtt caggcggggg aacgcgacgt cgcgcgctcc gatcatcagg gggatgaagt
     3001 agttgaagaa cgcggcgccg agcggcatga cgacgaagaa gaccatcgtc acgccgtgca
     3061 tcgtgaagat ctggttgtag gtgtccgcgt cgatcagcgc gccctcaggc gtggtgggcg
     3121 ccgcgaggtt gatccggatc agcagggcct cgacgcctcc cacgaggaag aagaagaacg
     3181 ccgtgacgcc gtacaggatc ccgatcttct tgtggtccac ggtcgtgaac cagctcgaga
     3241 acccggtggt cgcgctcggc cggcggggcc acggccggcg cgtcggcgcg gctacgactg
     3301 ccatcggcga cccatcctac tggccgtcct cggctccgag gctcatcagg taggcgacga
     3361 gcgcgtcgat ctgctcctcc gaaagtccgt agtcgggcat cacgacgccc ggcttcatcg
     3421 cgcgcgggtc gcgcagccac cgtcggaggt tcgcctcggt gttgtcgaag acccccgcgc
     3481 cgagcgtctc gcgctcgccg taccgggcca ggttcgggcc gacctggtcg ccgaccgttc
     3541 cgtcgacggt gtggcaggcg aggcaggaga acccgtcggg atactcgggg gtgacgaagc
     3601 gctggaagag ctcggcgcct tcagcttcga gggtcccctc ggcgggctcg ggggcggggg
     3661 agcgcatcga ccgcacccag gcgtcgaagt cctgctcggt gtgcgcgatc gcgcggagac
     3721 gcatgttcgc gtgcgagagg ccgcagaact cggcgcactg cccgaggtag atgccggggt
     3781 cctcggcttg gatcgtcatc ctcgtcgtcc ggcccgggag gacgtcctgc ttgccggcca
     3841 ggcgcgggat ccagaagctg tgcgccacgg ggatcgcgtt cttgtcgacg ccgctcgcgg
     3901 caggttcttc ggacgtcatc tcgaggtgca ccggctggtc gacgggcagg tggatctcgt
     3961 tcgccgtcac gatgccgagc tcggggtact ggaactccca ccaccactgg tgtccgatca
     4021 cccgcacgtt caccaggttc gggtcctccg gcgtctccgc gagggcgaag atcgtccgga
     4081 cggtcggcac cgcgatcacc gccaggatga tcgccgggat cgcggtccag atgatctcga
     4141 gccgcgtgtt gccgtgcacc tggaccgggt tggcggcgtc gcccggacgc cggcggaagc
     4201 ggaagacggc gaacacgatc agggcctcca ccaccacgaa gaccccgacc gcgatccaga
     4261 agaccggccg gaacaggttg tcgatctccc gcgcctgcgg ccccgccggg ttgagcgtgc
     4321 tcgccgggag gtcgtcccgg caggccgcga gcaggagggc gagcggcacc acgagcggca
     4381 ggagcgggcg cagcggcgat cgcatccggc gggaggtcac ggccgtacta gtagcacacc
     4441 ggcaccccta catcgaagcg cggtcgaggg cggccccgca ccgcgggcag cgcgggtcgg
     4501 cgcgcgaggc gacccggccg cagctcccgc agacgatcgc gcggacctgg ccccggatca
     4561 gcgcccgcac gaagaagaac gtgaagacgg ccgcgaggag cagggcggcg agctcgccga
     4621 tcgcgtgcca gccgtggcgg cggatcaggt cgatgaggaa cgcgagcggg ggactcacgc
     4681 cagcaccagc cggtccaccg ccgccgccgc gaacagcacg gcgaggtagg tgatcgagaa
     4741 gtggaacagc gccatcgcct cccggttgcc cgcgcccggg cgtcggaggc ggacggcctg
     4801 cgcgacgaac gcggcgccga gcgcgatggc cgtcgcgagg tagacggcgc cgagcgcggc
     4861 caccggcgcg aggaggagcg acgtcgcgag caggatgagc gagtagacga ggatctggcg
     4921 cttcgtcgcc tcgcgcccgc gcacgaccgg cagcatcggg atgcgggccg cctcgtactc
     4981 ggcctcgtgc cggaggctca acgcccagaa gtgtggcggg gtccacacga agacgatcgc
     5041 gaacagcacg accgccggca gctcgaccgt tcccgtcacc gcggcccagc cgatcagcgc
     5101 gggggcggcc ccggccgcgc ccccgatgac gatgttctgc ggggtcgagc gcttcatcca
     5161 gatcgtgtac acgacgacgt agaacgccgc cgcggcgaag gtgagggccg ccgccgggag
     5221 gttcaccgtg acggccagcc agatcgtccc ggccaggacg agcgcgatcc cgaaccgcag
     5281 cgcccctccc ggtgagaccc gatgcgcagg gatcggccga cggcgcgtcc ggaccatgac
     5341 ctcgtcgatg tcgcggtcga ggtagccgtt gatcgcgccg gccccgccgg ccgccagggc
     5401 gccgccgagc agcgtcgcgc ccacgagggc gcccgagggg atgccgccgg ccgcgaggac
     5461 catcgctggg acggtggtga tcagcagcag cacgatgatg tccggcttca tcagctggac
     5521 gtaggcgccg gcgacgctgc gggccgccgg gcgctcctgc cgcgcgacgc ctccgcgccc
     5581 ggcccgcgtc gcgacgacgg ttgcggtcac gagcgtcgcc caggcgaggc tcgagagggc
     5641 gacgtgcgcg gcgacggccg gcgtcgcgag ccgggtcagc accgccgccg ccccgacgag
     5701 cgcctggacc agcaggagcg cgaacgcccc ccacgcgagc cgacgcagcg cgacgttgcc
     5761 gctccggcgg gcgcgccagg cgaaggccgc gccgaagccg accgcgatca gcgcgagcgc
     5821 gcggtgcacg aagtgcgcgg tctcgccccg ctcgtcgagc gccggcagga agcgtccttc
     5881 catcaggggc cagtcgagga acgctaggct cgcgccggtc gcccgcacgt agatccccgc
     5941 gagcagcagg accgccgtga tcccgagggt ccaccacagc aagccgcgaa cgtcccggtc
     6001 gacggcgaca ccgctcccgc gcgggatcca cgacgcggcg gcgacgatca ccacgagcgc
     6061 gacgagcagc atcgcggtca ggaagtgggc catgttcatc gtcacgacct ggagctcgcc
     6121 gaccacgacc atgcgcccga gcgccgcctg gatgcccacg acgagcaccg cgacggccgc
     6181 cggccacacg atcgcccggt cgccgcgcgc gcggcgccac gcgaaccatg cggtcaggag
     6241 cacgaggacg ccgacgatcg cggccgtgag ccggtgggag tactcgatca gggcgtggaa
     6301 ctcgaagggc gggatgagcc ggccgtggca tcggggccag tcggggcagc ccaggccgga
     6361 tcccgtggcg cgcacgaccc cgccgaccgc gacgagggcg agcgcggcca ccgcggtgat
     6421 cgtcgaggtt cgcctcagca acgtcatcgg agccggcgcc gagaactata cgcgtcccgc
     6481 cgcggtataa agggaagcca tgccgcgccc ccgctccgtc gtgacgatcg cgctcgcgct
     6541 cgccttcgtg tccgcggcct gcggccagaa ggcgggcgtg cagcgcgacg ggagggtcgg
     6601 aggcgacccc gggccgaccg agccggcgcc gatcgctccg ctgccggcga ccgatgcggc
     6661 cggggcggta atggcgctct gcgcggcgga ggccgcggcg gccaccgggg acgtcgaggg
     6721 gatgcgcagc gccttcgaag accgggcgca cgcgttcctc catcagttcg ccgacacgct
     6781 ccaggcgacg gatcccgcga tcgccgccgg gctcctcgag gcgaagcagc aggtcgaggc
     6841 cgggctcgcg gacggaacgc cgcctccgga gatggccgca cgcctcgccg cccttcgctc
     6901 gcaggtcgag cagggcgcgg cggcgaacgg gacgccgacc caggggtgcg catgaggcgc
     6961 gcgctcgccc tttcgctcgc ggtcctcgcg ggagcctgcg gcggcggcgc cgaggatccg
     7021 ccggcggatc gacgcggtgg cgggctcgtc gcgcaggtcg cgagctacga gccgctggcg
     7081 ggagaggaca accgcctcat cgtcgggctg ctgaccgagg acaacctgtt cgtgagcggt
     7141 gggacggcga cgttccgctt ctcctacctc ggcgaggaag gggcggcgcc ggtgccgggg
     7201 ccggaggtgc aggccgggtt cctgccgatc gccgagcccg gcgcggtccc cccgccggag
     7261 cagccgacgg tgggcggcgc caccgacgtc gggcgcggcg tctacgcggc gcgcggggtc
     7321 cgcttcgacc gtgcggggct gtgggaggtc gaggtcatcg cgacgatcga cggcgaggag
     7381 cggagggcca cgtcggcgtt caccgtcctc gaggagccgc tcgtccccgg ggtcggcgag
     7441 cgtgccccgc gcacgaagaa cctgacgatc cacgacgggg acgcgccccc gcaggcgatc
     7501 gattcgcggg ccgagaccga gggcgaggtt cccgacccgg acctgcacga cacgacgatc
     7561 gccgccgcga tccggcgcgg ccggcccgtc ctggcggtct tcgccacccc cgtctactgc
     7621 gtcagccgct tctgcggccc gatcaccgac atggtcgccg gcctgcggga gcggtggggg
     7681 gatcgggtcg agatcatcca cgtcgagatc tggcgcgact tccaggagca ggtcgtgaac
     7741 aagggcgcgg cggagtgggt cctgcgcgag gacaacctca ccgagccgtg gatcttcctg
     7801 atcgacggcg acgggcggat cgcggcgcgc tgggacaacg tcgcgacgcc gggggagatc
     7861 gagcccgagc tcgagcgcgt caccggcggc tgacccctcg atgacgacgc tcgccgtcga
     7921 gccgttccag cttccgcccc gctgcgtcga gctcgcgggc cgatccgagg acgcgctgcg
     7981 ggggttcctc gaggaccagc ggaccgcgat cgtgggcccc gacccggggg cctccgccgc
     8041 gttcgacgag ctcgagcgcg tcgtgtgggc cggcgggaag cgcctgcgcc cggtgttctg
     8101 ctgcctcggg tacctcgcca ccggtgcgga agaagacgaa cgcctcttcg ccgccgcggc
     8161 cgcgctcgag ctcctgcaca ccttcgcgct cgtgcacgac gacgtgatgg accggtcgcc
     8221 aacgcggcgc ggaaccccga cgacctggat ccacctcgcg gagcgtcacc gccgggaggg
     8281 ctggcgcggc gatccggtcg cgtacgggat ctccgcggcg gtcctcgtcg gcgacctcgc
     8341 gatcgccctg gcggaccagg cgttcatgcg ctcgggcttc gcgtcggacc ggctcctgcc
     8401 cggcgcggtc cgctaccacc ggatgcggac cgacatggtc gcggggcagt tcctcgacgt
     8461 cgaggcgtcg cagcggaccg ggatctccga ggcggccgcc cgtcgcatcg cggtgctcaa
     8521 gtcgggcgcg tacaccgtcg agggccccct ccagatcggg gcgtcgctcg cgggctccgc
     8581 cgacggcctg ctcgatgcgc tctcccgcta cgggacggcg ctcggcgagg cgttccagct
     8641 tcgcgacgac ctcctcggat cgttcggcga cccgagcgtg acggggaagg accgggactc
     8701 ggacctgcgg gagggcaagc gcaccgtgcc cgtggcgctc gcgctggcgg ctgcgcccga
     8761 ggccgaccgc cgcttcctcg aagagcggct cggacacgcc gacctctccg atgacgaggt
     8821 cgagcggatg cgcggcatcg tcgagcgctc gggggccgtc gcggccacca tcggcctcgt
     8881 cgaggacctc agcgaggagg cgcgccggtc cgccgccgag gccccggtgc cgcccgaggt
     8941 ggcgtccctg ctggtctcca tggtcgacgc cctcgccgcg cgcccgatgt gagcaggagg
     9001 tcgttgcttc gccctggtcg ctgtgcgaca acctgatgcg tgaacctgct cgacgcgcgc
     9061 cgtgtcttcg ccccgctcca cgtgacgcag gtggcgacga cgcgcgtcga cggcagtccg
     9121 cacgtcgtcc cgctgtggtt cgtctggcgg gaggatgcga tctacgtctc gtgtcgccgc
     9181 gactccgtca cctggcgcgc ggtcgagcgg gaccgtcgcg tggcgctctc cttccacgtc
     9241 ggcgaggcgt ggagcgagct cgccggcgcc gtcgtctacg gccgcgcgga cccgatcgcc
     9301 gccgaccacc cggccctccg cggcgtcatg tcggcctggt tcgagaagta ccgctccctg
     9361 ctggccggcg ccgcgttcgc gcactacgcc cgcgaggtgg aggcgcccgg catgctgcgg
     9421 gtccgtcccc accgcctgat ctcctgggac cacgcgatcg ccgcccggcg cgcctcccag
     9481 cacgacgaag ccgccgacgc ctgagcgcgt tgccacctcg gcggccgcag gagtagcatg
     9541 cgcttgtgaa gattttcaca agcgccggga gggacgcttc agg
//
LOCUS       DATLGG010000274         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_121510, whole genome shotgun sequence.
ACCESSION   DATLGG010000274 DATLGG010000000
VERSION     DATLGG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121510"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VM638_07825"
     CDS             <1..626
                     /locus_tag="VM638_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HVM12366.1"
                     /translation="NDGFGSINLVDPDAAATAEVVARLLERIGGPLGGDVAACLYAGL
                     VTDTGRFQFEATTPEVLRLAARLREEPFDHVRLSQALYEDASLDYLRLLGTLLERIER
                     VPEADLLWTHLSRKDLEAAGIPIQETEDLIDVVRSAREADVAAVLKEQREGGYKVSLR
                     SKGKTDVSAIAAGFGGGGHRLAAGYSSSLDLRTTVDGLIGALVDARV"
     gene            633..1478
                     /gene="truB"
                     /locus_tag="VM638_07830"
     CDS             633..1478
                     /gene="truB"
                     /locus_tag="VM638_07830"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HVM12367.1"
                     /translation="MSVDGILLVDKPPGITSHDAIDRIRAALGVRKVGHAGTLDPMAS
                     GLLVVGVGRATRLLRFLGDLDKAYEGAGRLGVETDSLDAEGRVLREAEVDVSGEAVRA
                     AAAGLVGSIDQRPPAYSAVKVGGRRLYDAARRGERVEAAPRRVRVDAFEVTRLDLPRF
                     DFLVVCSAGTYVRVLVADVGSALGCGAHLTRLVRTRIGHFHLEDARPPDDPGEPLPLE
                     RAVAHLPRVGVSEDEAAAARHGRPLAPAGVAGMHAVVDPQGRLVGVYRDDGARARPEV
                     VIPAL"
     gene            1512..>3200
                     /locus_tag="VM638_07835"
     CDS             1512..>3200
                     /locus_tag="VM638_07835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HVM12368.1"
                     /translation="MRRRSLGVSLLLSLVAGMGTALPAGAAPGLPEHVPGEVLVRFEP
                     GVSSAERAALRARVAAREATPLDGLPKVELLRLPPGRSVADAVAALEARPEVRYAQPN
                     FVYRLEQTIPNDTHFQYLWGLHNIGQSGGVADADIDAPEAWSAATGSSDVMVAVIDTG
                     VDPTHLDLQGNLAPGGKDWVANDMDPADEHGHGTHVAGTIGARGDNGRGVTGVSWRVS
                     ILPLRVLNAQGRGFSAHIASAIAYAAEQGADVINLSLGGASRDPATAEAIRGASRSLV
                     VAAAGNDGTDNDVSAVYPCNEGQKGAANVVCVAATNRHDERASFSNFGATAVHLGAPG
                     QGILSTTRGGGYGSMSGTSMATPHVAGAAALLWSAAPGASVSEVKGRLLGNVDPIPSL
                     AGVTVTGGRLNVAAALAASGEEVAPAAPIAEIEPAGGIQRAPTFTVRWGSPDVERYDV
                     RFREAGVITSFATPDDWLDGTTETSASFDGSPASTYCFSARDDASEGAAWGEERCTAV
                     PMNDRALKPRGTWSRRTAAGTYLGTVSVARERGSALSADVRAQRLAVVATRCPSC"
BASE COUNT          428 a         1100 c         1238 g          434 t
ORIGIN      
        1 cgaacgacgg gttcgggtcc atcaacctcg tggaccccga cgcggccgcg acggccgagg
       61 tcgtcgctcg gctgctcgag cggatcggcg gtccgctcgg cggggacgtc gccgcctgcc
      121 tctacgccgg cctcgtcacc gacaccgggc ggttccagtt cgaggcgacg acgccggagg
      181 ttctccgtct ggcggcgcga ctacgcgagg agccgttcga ccacgtccgc ctctcccagg
      241 cgctgtacga ggatgcctcg ctggactacc tccgcctcct gggcacgctg ttggagcgga
      301 tcgagcgtgt gcccgaggcg gatctcctgt ggacgcacct ttctcggaag gacctcgagg
      361 ccgcgggcat cccgatccag gagaccgagg atctgatcga cgtcgtccgc agcgccaggg
      421 aggccgacgt cgccgcggtg ctcaaggagc agcgggaggg cggctacaag gtctcgctcc
      481 ggtcgaaggg caagaccgac gtctcggcga tcgccgcggg gttcggtgga ggcggtcatc
      541 ggctcgctgc cggatattcc tcgagcctcg acctcaggac gacggtggat gggctcatcg
      601 gcgccctcgt cgacgcgcgc gtctagccga gggtgagcgt cgacgggatc ctgctggtcg
      661 acaagccccc gggcatcaca tcgcacgatg cgatcgatcg gatccgcgcg gcgctcggcg
      721 tccgcaaggt gggtcacgcg gggacgctcg acccgatggc ctcgggcctc ctcgttgtcg
      781 gcgtggggcg cgcgacccgg ctcctgcggt tcctcggcga tctcgacaag gcgtacgagg
      841 gggcgggtcg tctcggcgtc gagaccgaca gcctcgacgc ggagggacgg gtgctccgcg
      901 aggccgaggt ggacgtgtcg ggcgaggcgg tccgcgccgc ggcggcgggt ctcgtggggt
      961 cgatcgacca gcgacccccc gcgtactctg cggtgaaggt cgggggcagg aggctctacg
     1021 acgcggcgcg ccggggagag cgggtcgagg cggccccgcg gcgcgtccgg gtcgacgcgt
     1081 tcgaggtgac ccggctcgac ctgccgagat tcgacttcct cgtcgtgtgc tcggccggca
     1141 cctatgtgcg ggtgctggtg gcggacgtcg gatcagcgct cggctgcggg gcccatctga
     1201 cgaggctcgt tcgcaccagg atcggccact tccacctcga ggacgcgcgg ccgccggatg
     1261 acccggggga gccgctgccc ctcgagcgtg cggtggcgca cctccctcgg gtcggggtga
     1321 gcgaggatga ggcggcggcc gcgcggcacg ggcggccgct ggccccggcg ggcgtggccg
     1381 gcatgcacgc cgtggtggat ccgcagggtc ggctcgtcgg ggtctatcga gacgacggcg
     1441 cgcgggcccg tcccgaggtc gtcatcccgg ctctctaagg aagatcgagg gcgatgccga
     1501 atataagacc catgcgtcgc cgctccctcg gggtctccct cctgctgtcg ttggtggccg
     1561 ggatggggac cgctcttccg gcgggcgcgg cacccgggct ccccgagcat gtgccggggg
     1621 aggtcctcgt gcgcttcgag cccggcgtct catccgccga gcgcgccgcc cttcgggcgc
     1681 gcgtcgccgc ccgcgaggcg acccccctcg acgggctccc gaaggtcgag ctgctccggc
     1741 tcccgccggg gcgaagcgtg gcggatgccg tggcggcgct cgaggcgcga ccggaggtgc
     1801 ggtacgccca gcccaacttc gtgtaccggc tggagcagac catcccgaac gacacccact
     1861 tccagtacct ctgggggctc cacaacatcg gccagtcggg cggggtcgcc gacgccgata
     1921 tcgatgcgcc cgaggcgtgg tcggcggcca cggggagcag cgacgtcatg gtcgcggtca
     1981 tcgacaccgg cgtcgacccg acacacctcg atctgcaggg gaacctcgcg ccgggaggga
     2041 aggactgggt cgcgaacgac atggatcccg cggacgagca cggccacggg acccacgtcg
     2101 ccggcacgat cggggcccgg ggcgacaacg gccgcggggt gaccggggtg agctggcggg
     2161 tgtcgatcct gccgttgcgc gtcctgaacg cgcaagggag ggggttcagc gctcacatcg
     2221 cgtcagcgat cgcctacgcg gcggagcagg gagcggacgt gatcaacctg tcgctggggg
     2281 gcgccagccg ggatcccgcg accgccgagg cgatccgcgg cgcttcccgc agcctcgtcg
     2341 tggccgcggc gggcaacgac gggaccgaca acgacgtgag cgcggtctac ccgtgcaacg
     2401 aaggccagaa gggcgcggcg aacgtcgtct gcgtcgcggc gacgaaccgc cacgacgagc
     2461 gggcatcgtt ctccaacttc ggcgcgacgg cggtccacct cggggcgccg ggccagggca
     2521 tcctcagcac gacccgcgga ggcgggtacg gatcgatgag cgggacctcg atggcgacgc
     2581 cgcacgtcgc gggcgccgcg gcgctgctgt ggtcggcggc tccgggggcg agcgtctccg
     2641 aggtgaaggg ccgcttgctc gggaacgtcg acccgatccc atccctcgcc ggggtgaccg
     2701 tcaccggggg gcggctgaac gtggccgcgg cgctggcggc gagcggcgag gaggtggcac
     2761 ccgcggcgcc catcgcggag atcgagcccg cgggaggcat ccagcgcgcg ccgacgttca
     2821 ccgtccgctg ggggtcgccg gatgtcgagc ggtacgacgt ccgcttccgc gaggcaggag
     2881 tgatcacgag cttcgccaca cccgatgact ggctggatgg gacgaccgag acgagcgcct
     2941 cgttcgatgg gtcaccggcg tccacctact gtttctcggc ccgcgacgac gcgagcgagg
     3001 gggcggcatg gggggaggaa cgctgcaccg ccgtgccgat gaacgaccgg gcgctgaagc
     3061 cccgagggac ctggtcgcgg cgcacggcag cgggcacgta cctcgggacg gtttccgtcg
     3121 cccgggagcg gggctctgcc ctgagcgccg acgtccgggc acagcggctc gcggtggtcg
     3181 ccacccggtg cccctcgtgc
//
LOCUS       DATLGG010000275         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_76762, whole genome shotgun sequence.
ACCESSION   DATLGG010000275 DATLGG010000000
VERSION     DATLGG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76762"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="VM638_07840"
     CDS             <1..1745
                     /locus_tag="VM638_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12369.1"
                     /translation="PQGLIPEDAYIRPTSAEGPSGWTASLRDVPPSVEFLAPAGSGIQ
                     PGQAATFTVTGDVGRLAADECSEWTIRVSSDGFDSTAIAEAAASGATTTCIRVLEATG
                     LALVAPAGAADGTVTAGQSNACARLTLRNEGTADLVVLPRPEGNGVGVGLARAAADPP
                     CTGSELAEGKAPMASGETRSFDFRLSFGDAASTLLYGRATASGATAVERALPLTIQPQ
                     ASLTYVNGTLIPRSVQPRTPGAVFRFTATKGPAGSPAISLDPAATTFSFAACPPQPLI
                     SQPGVGPGQSPNVVLEFDACDIEPVQDGRYDTAVRYRYTDANGFTSETQLAGVDRVRI
                     DGTPPQVDLSLTLPTPACCVPPEKRAVKDGDRVTLTGKVRDPDPDSGQTQACPGCLVQ
                     GEIRQYQDAGCELAGAPAIATTPANQGGDLGAVYQGSYHVTTAGIRWVVSARDDAGNL
                     RVASSDCVEVDNLRPLLAKAETARVGAEQNRVKVTVEECVGGTSNPLDWRIEGNLVVE
                     VESITCAIVGGVPRGAITLRTILPFPDDAPLGRVRYEPLPLVGGRVFDRVNNTMSAQD
                     VPLEDGISRRIVGL"
     gene            1885..>2776
                     /locus_tag="VM638_07845"
     CDS             1885..>2776
                     /locus_tag="VM638_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12370.1"
                     /translation="MRRVRPALLLAAVVVASTASPAAAYKRPGKTERISVSTSGAQVT
                     GHNIDAAISPDGRFVVFASSASTLVPGDTNGAFDVFVRDLGAHVTERVSVASDGKEAN
                     GPSASGFSYYRAKITPGGRFVVFASDASNLVPGDTNDQTDVFVHDRLTRKTELVSAPA
                     GGGVGDRSSVYPAISTDGRYVAFVSWATNMVPGDTNEAGDVFVRDRLAQRTERVSLAT
                     SGAEANGLSLPAFISGDGRFVAFGSSASNLVPNDNNATSDVFVRDRATGTTERVSVSV
                     LGGNANGVSNAGGISDDGRFV"
BASE COUNT          420 a         1010 c          971 g          375 t
ORIGIN      
        1 ctccccaggg actgatcccg gaggacgcct acatccgacc cacgtcggcg gagggcccgt
       61 ccggctggac ggccagcctg cgcgacgtcc cgccgtcggt cgagttcctc gcccccgccg
      121 gatcgggcat ccagccggga caggcggcga cgttcacggt caccggcgac gtcggccggc
      181 tcgcggcgga cgagtgctcg gagtggacga tccgcgtctc gagcgacggc ttcgactcca
      241 ccgcgatcgc tgaagcggcg gcgagcggcg ccacgacgac ctgcatccgc gtcctcgagg
      301 cgaccgggct cgcgctcgtc gcacccgccg gggccgcgga cggaaccgtc acggccggcc
      361 agtcgaacgc ctgcgcgcgg ctgacgctcc ggaacgaggg caccgcggac ctcgtggtgc
      421 tgccccgacc ggagggcaac ggggtgggcg tcggcctggc ccgggccgcg gccgaccctc
      481 cctgcacggg atccgagctc gccgagggga aggcgccgat ggcgtccggc gagacccgga
      541 gcttcgactt ccggctgagc ttcggcgacg ccgcctccac gctcctctac ggccgcgcga
      601 ccgcgtccgg cgccaccgcg gtggagcgcg cgctcccgct cacgatccag ccgcaggcga
      661 gcctcaccta cgtgaacggg acgctgatcc cgcgctcggt ccagccgcga acacccggcg
      721 cggtgttccg cttcaccgcc acgaagggcc ccgcgggctc gccggcgatc tccctcgacc
      781 cggccgccac gaccttctcc ttcgccgcct gcccgccgca gccgctgatc tcccagccgg
      841 gcgtcggccc ggggcagtcg ccgaacgtcg tcctcgagtt cgacgcctgt gacatcgaac
      901 cggtccagga cggccgctac gacaccgccg tccgctaccg ctacaccgac gccaacgggt
      961 tcacgagcga gacccagctc gcgggggtgg atcgcgtgcg catcgacgga acgcctccgc
     1021 aggtcgacct ctcgctgacg ttgccgacgc ccgcctgctg cgttccgccg gagaagcggg
     1081 cggtcaagga cggggaccgc gtcacgctga ccggaaaggt ccgggacccc gaccccgaca
     1141 gcggtcagac ccaggcgtgc ccgggttgcc tcgtgcaggg tgagatccgg cagtaccagg
     1201 acgccggatg cgagctcgcc ggcgctccgg ccatcgcgac gacccccgcg aaccagggcg
     1261 gcgacctcgg cgcggtctac cagggctcct accacgtgac gaccgccggg atccgctggg
     1321 tcgtctcggc ccgtgacgac gccggcaacc tccgggtcgc gtcgagcgac tgcgtcgagg
     1381 tcgacaacct gcggcctctc ctggccaagg ctgagacggc ccgggtcggc gccgagcaga
     1441 accgcgtgaa ggtcacggtc gaagaatgcg tcggcggcac gagcaacccc ctcgactggc
     1501 gcatcgaggg gaacctcgtc gtcgaggtcg agagcatcac gtgcgcgatc gtcggcggcg
     1561 tcccgagggg cgcgatcacc ctccggacga tcctcccgtt cccggacgac gctccgctcg
     1621 gcagggtccg gtacgagccc ctgccgctcg tgggcggacg ggtcttcgac cgggtcaaca
     1681 acacgatgag cgcgcaggac gtccccctcg aggatgggat ctcgcgcagg atcgtcgggc
     1741 tgtgaccggt tcaccccgtt cgccccggtg tgctcgtcga acactgtgca actgcccaaa
     1801 ggattcgaag gagcgaccct cgaatctgat gggagcgcga gaggaagggc agatgtgcag
     1861 gcgcggcgag ggaggggtgg agcggtgagg cgcgtccgac cggcactgct gctcgcggcg
     1921 gtcgtcgtcg cgagcacggc gtctccagcc gcggcctaca agcgaccggg gaagaccgaa
     1981 aggatctcgg tctcgacgtc cggcgcgcag gtcacgggtc acaacatcga cgcggcgatc
     2041 agcccggacg gccggttcgt cgtcttcgcg agctccgcgt ccacgctcgt cccgggtgac
     2101 acgaacggag ccttcgacgt cttcgttcgc gatctcggcg cgcacgtcac ggaacgtgtt
     2161 tcggtcgcct ccgacgggaa ggaagcgaac ggcccgtccg cgagcgggtt ctcctactac
     2221 cgcgccaaga tcactccggg cggccgattc gtcgtcttcg cgagcgacgc gtccaacctc
     2281 gttccgggag atacgaacga ccagacggac gttttcgtcc acgaccgcct gaccaggaag
     2341 accgagctgg tctcggcgcc cgccgggggc ggggtggggg accgctcgtc cgtctaccct
     2401 gcgatcagca cggacgggcg ctacgtcgcc ttcgtcagct gggcaacgaa catggtgcca
     2461 ggcgacacca acgaggccgg ggacgtcttc gtccgggatc gactcgccca gaggacggag
     2521 cgcgtttcgc tggcgacctc gggtgccgag gccaacgggc tgtcgctgcc ggcgttcatc
     2581 agcggcgacg gacggttcgt ggcgttcgga agctcagcga gcaacctcgt tcccaacgac
     2641 aacaacgcga ccagcgacgt cttcgtgcgc gatcgcgcga ccggtacgac cgagcgggtc
     2701 tccgtttcgg tcctcggagg gaacgcgaac ggtgtctcca acgccggagg gatcagcgac
     2761 gatggccgct tcgtcg
//
LOCUS       DATLGG010000276         7822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_76822, whole genome shotgun sequence.
ACCESSION   DATLGG010000276 DATLGG010000000
VERSION     DATLGG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76822"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="clpX"
                     /locus_tag="VM638_07850"
     CDS             <1..952
                     /gene="clpX"
                     /locus_tag="VM638_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HVM12371.1"
                     /translation="SNILLLGPTGCGKTLLAQTLARMLNVPFAIADATALTEAGYVGE
                     DVENILLKLIQAADYDVKRAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKI
                     LEGTTASVPPQGGRKHPHQEFIQIDTTNILFICGGAFAGIEKIIEDRVGNRGVGFGAD
                     VRRADQKDLGEILGRVLPEDLLKYGLIPEFVGRLPVITHVHNLDKDALVDILTKPKNA
                     LVKQYHRFFDFDGVELVFEDDALEAVADQSILRGTGARGLRAILEEVLLGVMYDLPSQ
                     KDVGKCVINAEVVLKGVNPTLVPRTTRAKGTPPQRKERSA"
     gene            997..1200
                     /locus_tag="VM638_07855"
     CDS             997..1200
                     /locus_tag="VM638_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12372.1"
                     /translation="MATTDDLTVRPATSSEDELVDRLACALLWVDLAAISLHAGGSGR
                     TAHGLTQARAHLRAAIRHLKSPS"
     gene            complement(1197..1955)
                     /locus_tag="VM638_07860"
     CDS             complement(1197..1955)
                     /locus_tag="VM638_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVM12373.1"
                     /translation="MIRIAAAGDLHYGVDSAGRMRPALADLDDHADILCLAGDLTTRG
                     RPEEAEVLARELADAPVPVVAVLGNHDYHTDQQQEVTEILERAGARVLEGTSLVHEVN
                     GVRVGIAGVKGFGGGFAGASGSEFGEPEMKLFMRVTIDAANRLDAALTEMDADLKIAL
                     MHFSPIPETLEGEPLEIYPFLGSYLLAEPLDKHRVDLALHGHAHRGDEHGETPGGVPV
                     RNVAQHVLEAPYKVYSLDPENAERRDRSDATIGS"
     gene            complement(1952..2197)
                     /locus_tag="VM638_07865"
     CDS             complement(1952..2197)
                     /locus_tag="VM638_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein"
                     /protein_id="HVM12374.1"
                     /translation="MIEEYTVEAIRRALARDPRVGELELDVEVDEEAIRVTGTLPTEQ
                     RRDAVTEVLEERFPNHRVENATAVLSPEAPPDEEVVG"
     gene            complement(2194..2862)
                     /locus_tag="VM638_07870"
     CDS             complement(2194..2862)
                     /locus_tag="VM638_07870"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HVM12375.1"
                     /translation="MASTGHPARTASKKPPEPGPYDPGPHGASEEPFATILGEVTSAL
                     DEAGIDAVVFGGVAAVTYGRPRGTKDIDVLVRPAEAERTLEVLDAHGFQTEHTDERWL
                     YKAYKSDVMVDVIFRVKGEIYLDDEMVERSTLVQINGHSARLLSREDALVIEAVSHDD
                     EHPDHWYNALAIIGRGELDWDYVRRRARLGPRRLLALLVYAQSNDLMVPDEEVKSLFD
                     SVYR"
     gene            2929..5562
                     /locus_tag="VM638_07875"
     CDS             2929..5562
                     /locus_tag="VM638_07875"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HVM12376.1"
                     /translation="MGEMGTTYDPKGVEPRRYATWVERGYFRAEAGSEREPFCIVLPP
                     PNITGALHIGHALDHTIQDVVIRRKRMQGYEALWVPGTDHAGIATQVVVERELAKEGI
                     DRRELGREGFLERVWAWKARYGDRIVEQMKALGNSCDWDRLAFTMDEARSRAVRVAFV
                     RLYEAGLVYRGERLVNWCPKDQTALSDSELEHEDVEGELVTLRYRRADGGGHVDVATT
                     RVETMLGDTGIAVHPDDERYRAVVGTSVIHPFDGREIPIVADVAVDPSFGTGAVKVTP
                     AHDTGDFEIAERTGLPLINIFNADATLNDAVPEEFRGLDRYEARRRVRERLDDMGHVV
                     DEERPYVHAVAHCYRCRSEIEPWLSGKQWFVKVGPLVGPAKAAAEDGRIRFHPERWRG
                     PYGAWLDNLRDWNISRQLWWGHRIPVWYCDNGHEIAAIEDPDACVECGSTKLEQDPDV
                     LDTWFSSQLWPYSTLGWPDDTDDLRTFYPTSVLVTGYEILYLWVARMIMSGLFLVGDV
                     PYGEVVIHGLVRDRTGRKMSKSLGNVIDPLDMIERYGADALRFALARMASPEQQNLPL
                     AEESIEAARHFANKIWNAARLVLSRDARAELPPAERLTIGDRWLLSRHEACRAEVDEA
                     LDAYRLDDAAQAIHRFLWSEYCDWGLELAKQRLYEGSEEEAGDAAAVLTWVLERTLRL
                     LHPIMPFVTEEIWERVGAGETIVRAPWPEERPEDRDPAAEDGFGFVQDLVVAVRRFRS
                     SHGLGPGHELDVRVRADEGQRELLGMFREELRRLGRLGRIDEVETLEAAGAARLLVRG
                     AEVAVPLAGVLDVETECDRLRRRLEAVAADMAKVEAKLGNEAFVTKAPAEVVEKERGK
                     LEEGREERAALEAQLAELGCA"
     gene            5564..6841
                     /locus_tag="VM638_07880"
     CDS             5564..6841
                     /locus_tag="VM638_07880"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HVM12377.1"
                     /translation="MEFADVLADLEARRPERVKPSLDRIRALTDLLDDPQHRYRTVHV
                     AGTNGKTTTARLIGRILCAHGATAGVYTSPHLTSVTERLALCDQPIWGDEMAAVYTHL
                     KPYLDEVARRGDPPSYFETLTALAFLWMADKPVEIGVFEVGMGGTWDATNLVRGEVAA
                     ICRIGLDHAELGSTVEEVAAEKAGVIKEGATVVVEEQPEDALRVIEARAAEVGAEVRV
                     EGDAFAVEDRELAVGGQRLAIRSTGRYEDLFLPLHGPHQAHNAAVAVAAAEAVLESDL
                     EEDVLREALAGARSPGRLEVVSSDPLVLLDGAHNPDGAEALTRALGEGFVWRRLHLVI
                     GVLREKDLDGILEPLAARVDVAYACSTSNPRVRDPKDVAAACLRAGIPATEHASVVDA
                     LDAAHAVAAEDDVILVTGSLSTVGDARPRYAPA"
     gene            6883..7287
                     /gene="ndk"
                     /locus_tag="VM638_07885"
     CDS             6883..7287
                     /gene="ndk"
                     /locus_tag="VM638_07885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HVM12378.1"
                     /translation="MATEETLVIVKPDGVRRRLVGEVLARIERKGLTIEELRMMRIDR
                     ELAERHYGEHREKPFFGELVEFITSGNVVVARVSGESAIPVVRTLMGPTNPAEAPPGT
                     IRGDFGTIITENLVHGSDSPESAKRELALFFD"
     gene            7416..>7822
                     /locus_tag="VM638_07890"
     CDS             7416..>7822
                     /locus_tag="VM638_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12379.1"
                     /translation="MSVTLTRALRWSVLMISIAAVAAGTVPVAASPGGAQELPVGLGV
                     SVGDPREVGANASDVDRVESHSSGPRWYEASEWAGPTQNCIHGYSEQLGMAWNGWYGE
                     IGSSPTVGNVYYVKVGWGVSGNPCGGGAKVHVE"
BASE COUNT         1158 a         2716 c         2802 g         1146 t
ORIGIN      
        1 gtcgaacatc ctgctgctcg gcccgaccgg ctgcgggaag acgctcctcg cgcagaccct
       61 cgcgcgcatg ctgaacgtcc cgttcgcgat cgccgacgcc accgcgctca ccgaggctgg
      121 ctacgtcggg gaggacgtcg agaacatctt gctgaagctg atccaggctg ccgactacga
      181 cgtgaagcgg gcggagaccg ggatcatcta catcgacgag gtcgacaaga tcgcgcggaa
      241 gtccgagaac ccctcgatca cgcgcgacgt ctcgggggag ggcgtccagc aggcgctctt
      301 gaagatcctc gaggggacga ccgcgagtgt cccgccgcag ggcgggcgca agcaccccca
      361 ccaggagttc atccagatcg acacgacgaa catcctgttc atctgcggcg gcgccttcgc
      421 cgggatcgag aagatcatcg aggaccgcgt cggcaaccgc ggcgtcggct tcggcgcgga
      481 cgtgcggcgt gcggaccaga aggacctcgg cgagatcctc ggccgcgtgc ttccggagga
      541 cctcctgaag tacgggctga tccccgagtt cgtcggacgg ctcccggtca tcacccacgt
      601 gcacaacctc gacaaggacg ccctcgtcga catcctcacg aagccgaaga acgcgctcgt
      661 caagcagtac caccgcttct tcgacttcga cggcgtcgag ctcgtgttcg aggacgacgc
      721 gctcgaggcc gtggccgacc agtcgatcct ccgcggcacc ggcgcgcgcg gcctccgcgc
      781 gatcctcgag gaggtgctgc tcggcgtgat gtacgacctg ccgtcgcaga aggacgtcgg
      841 caagtgcgtg atcaacgccg aggtcgtcct gaagggggtc aaccccacgc tggttccccg
      901 gaccacccgc gcgaagggca ctccgcccca gcgcaaggaa cgcagcgcct agaccgcggc
      961 tacaggttcg ccggggccga cccgatacac ccttcgatgg cgacgaccga tgacctcacg
     1021 gtgcggccgg ccacatcttc cgaggacgag ctggtggatc gcctcgcctg cgcgctcctg
     1081 tgggtggacc tggccgcgat ctcgctccac gcggggggat ccggccgcac ggcccacggc
     1141 ctcacccagg ctcgtgccca tctacgcgcc gcgatccgtc acctgaagtc tccgtcctag
     1201 gagccgatcg tggcgtcgct ccggtcacga cgctcggcgt tctccggatc gagcgagtag
     1261 accttgtacg gggcttcgag gacgtgctgg gcgacgttgc ggaccggcac gccgccgggg
     1321 gtctcgccgt gctcatctcc ccggtgcgcg tggccgtgga gcgcgaggtc gacgcggtgc
     1381 ttgtcgagcg gctccgccag caggtagctc ccgaggaacg gatagatctc cagcggctcc
     1441 ccctcgagcg tctccgggat gggggagaag tgcatcagcg cgatcttcag gtccgcatcc
     1501 atctcggtga gcgctgcgtc cagccggttc gccgcgtcga tcgtcacgcg catgaagagc
     1561 ttcatctccg gttcgccgaa ctcgctgccc gacgcgccgg cgaacccgcc gccgaacccc
     1621 ttcacgccgg cgatgccgac gcgcaccccg ttgacctcgt gcacgagcga ggtgccctcg
     1681 aggacccgcg caccggcgcg ctccaggatc tccgtgacct cctgctgctg gtccgtgtgg
     1741 tagtcgtggt tcccgagcac ggccacgacc gggaccggcg catcggcgag ctcccgcgcc
     1801 agcacctccg cctcctcggg acggccccgc gtcgtcaggt cgcccgcgag gcacaggatg
     1861 tccgcgtgat cgtcgaggtc ggcgagcgct gggcgcatcc ggccggcgga gtcgacgccg
     1921 taatggaggt cgccggcggc ggcgatgcgg atcatccgac gacctcctcg tcgggtggcg
     1981 cctccggcga gagcaccgcc gtcgcgttct cgacccggtg gttggggaag cgctcctcca
     2041 ggacctcggt cacggcatcc cgccgttgct ccgtgggaag cgtccccgtc acgcggatcg
     2101 cttcctcgtc gacctcgacg tcgagctcca gctcgccgac gcgcgggtcc cgagccaggg
     2161 cgcgccggat cgcctcgacg gtgtactcct cgatcatcgg tagaccgaat cgaagaggct
     2221 cttgacctcc tcgtcgggaa ccatgaggtc gttcgactgc gcgtacacga gcagggccag
     2281 caggcggcgc ggtcccagcc tcgcgcgccg gcggacgtag tcccagtcca gctcgccgcg
     2341 cccgatgatc gcgagggcgt tgtaccagtg atcggggtgc tcgtcgtcgt ggctcaccgc
     2401 ttcgatcacc aaagcgtcct ctcgcgagag gaggcgcgcg ctgtggccgt tgatctggac
     2461 gagcgtggaa cgctcgacca tctcgtcgtc gaggtagatc tcgcccttca cccggaagat
     2521 cacgtcaacc atcacgtcgc tcttgtacgc cttgtagagc cagcgctcgt cggtgtgctc
     2581 ggtctggaac ccgtgcgcgt cgaggacctc cagggtccgc tccgcctccg cggggcggac
     2641 gagcacgtcg atgtccttcg tccctcgggg gcggccgtag gtgacggctg ccactccccc
     2701 gaagaccacg gcgtcgatcc cggcctcgtc gagcgcgctc gtgacctcgc cgaggatcgt
     2761 cgcgaagggc tcttcgctcg ccccgtgggg cccggggtcg tacggaccag gctcgggggg
     2821 cttcttggag gccgtgcgtg cgggatgacc ggtggatgcc acgtcgtgac cgtttccagc
     2881 agaggtggct cctaaacggc cgccgcccgg cgggtagact cgcgggccat gggcgagatg
     2941 ggcaccacgt acgacccgaa gggcgtcgag ccgcgccggt acgccacctg ggtggagcgc
     3001 ggctacttcc gcgccgaggc cggctctgaa cgggagccct tctgcatcgt cctgccgccc
     3061 ccgaacatca cgggcgccct gcacatcggg cacgcgctcg accacacgat ccaggacgtc
     3121 gtgatccgcc ggaagcggat gcagggctac gaggccctgt gggtccccgg gaccgatcac
     3181 gcgggcatcg cgacccaggt cgtggtcgag cgggagctgg cgaaggaggg gatcgaccgc
     3241 cgggagctcg ggcgcgaggg gttcctcgag cgggtctggg cctggaaggc gcgctacggc
     3301 gaccggatcg tcgagcagat gaaggcgctc ggcaactcct gcgactggga ccggctcgcg
     3361 ttcacgatgg acgaggcgcg ctcccgcgcc gtccgcgtcg cgttcgtccg gctgtacgag
     3421 gccgggttgg tctaccgcgg ggagcggctc gtgaactggt gcccgaagga ccagacggcg
     3481 ctgtcggact ccgagctcga acacgaggac gtcgagggcg agctcgtcac gctccggtac
     3541 cggcgcgccg acggaggggg ccacgtcgac gtcgccacga cgcgtgtcga gacgatgctc
     3601 ggtgacaccg ggatcgcggt gcaccccgac gacgagcgct accgcgccgt cgtcgggacg
     3661 agcgtgatcc accccttcga cgggcgcgag atcccgatcg tggccgacgt cgccgtcgat
     3721 ccttcgttcg gcaccggcgc cgtcaaggtc acccccgccc acgacaccgg cgacttcgag
     3781 atcgcggagc ggactgggct cccgctcatc aacatcttca acgccgacgc gacgttgaac
     3841 gacgcggtcc ccgaggagtt ccgcgggctc gaccgctacg aggcgcgacg ccgcgtgcgc
     3901 gagcgcctcg acgacatggg gcacgtcgtg gacgaggagc gcccgtacgt gcacgcggtc
     3961 gcccactgct accgctgccg cagtgagatc gagccgtggc tctcgggcaa gcagtggttc
     4021 gtcaaggtcg gcccgctcgt cggtccggcg aaggcggccg cggaggacgg acggatccgc
     4081 ttccaccccg agcgctggcg cggtccctac ggcgcgtggc tcgacaacct ccgcgattgg
     4141 aacatctcgc gacagctctg gtggggccac cggatcccgg tgtggtactg cgacaacggt
     4201 cacgagatcg ccgcgatcga ggacccggac gcgtgcgtcg agtgcggttc gacgaagctc
     4261 gagcaggacc cggacgtcct cgacacgtgg ttctcgtccc agctctggcc gtactcgacc
     4321 ctcggatggc ccgacgacac cgacgacctt cgcacgttct atcccacctc ggtgctcgtg
     4381 accggctacg agatcctcta cctctgggtc gcgcggatga tcatgtcagg gctcttcctc
     4441 gtcggcgacg tgccgtacgg cgaggtcgtg atccacggcc tcgtgcgcga ccggaccggc
     4501 cggaagatgt cgaagtcgct cggcaacgtg atcgacccgc tcgacatgat cgagcgctac
     4561 ggcgcggacg cgctccggtt cgcgctcgcg cggatggcga gccccgagca gcagaacctg
     4621 ccgctcgccg aggaatcgat cgaggcggcg cgccacttcg cgaacaagat ctggaacgcg
     4681 gcgaggctcg tcctctcgcg cgatgcgcgc gccgagctgc cgcccgcgga gcgtctgacg
     4741 atcggcgatc gctggctgct ctctcggcac gaggcctgcc gcgccgaggt ggacgaggct
     4801 ctcgacgcct accgtctcga cgacgccgcg caggcgatcc accgcttcct gtggtcggag
     4861 tactgcgact gggggttgga gctcgcgaag cagcgcctgt acgaggggag cgaggaagaa
     4921 gcgggtgacg cggccgccgt cctgacctgg gtcctcgagc ggaccctgcg gctgctgcac
     4981 ccgatcatgc cgttcgtcac cgaggagatc tgggagcggg tcggcgcggg cgagacgatc
     5041 gtgcgcgcgc cgtggcccga ggagcggccg gaggaccgcg acccggccgc cgaagacggg
     5101 ttcggcttcg tccaggatct cgtcgtcgcg gtccggcggt tccgcagcag ccacgggctc
     5161 ggccccggcc acgagctcga cgtgcgggtg cgcgcggacg agggtcaacg ggagctgctg
     5221 ggcatgttcc gtgaggagct ccggcggctg gggaggctcg gccgcatcga cgaggtcgag
     5281 accctcgagg cggccggggc cgcgcgtctg ctcgttcggg gcgccgaggt cgcggtcccc
     5341 ctcgccgggg tcctcgacgt cgagacggag tgcgaccgcc tgcgccgccg gctcgaggcg
     5401 gtggccgcgg acatggccaa ggtcgaggcg aagctcggga acgaggcgtt cgtcaccaag
     5461 gcccccgccg aggtcgtgga gaaggagcgc ggcaagctcg aggaaggtcg ggaggagcgg
     5521 gcggccctcg aggcccagct cgccgagctc ggctgcgcct gacgtggagt tcgccgacgt
     5581 cctcgccgac ctcgaagcgc gccgcccgga gcgcgtcaag ccctcgctcg accggatccg
     5641 cgccctcacc gacctgctcg acgaccccca gcaccgctac cgcaccgtcc acgtcgccgg
     5701 gacgaacggc aagacgacga ccgcgcggct gatcggccgc atcctctgcg cgcacggtgc
     5761 gacggccggc gtctacacct cgccccacct cacgtcggtc accgagcggc tcgcgctgtg
     5821 cgaccagccg atctgggggg acgagatggc ggccgtctac acgcacctga agccctacct
     5881 cgacgaggtc gctcgccgcg gcgatccgcc gagctacttc gagacgttga cggcgctggc
     5941 cttcctgtgg atggcggaca agccggtgga gatcggcgtg ttcgaggtcg ggatgggcgg
     6001 gacctgggac gcgacgaacc tcgtccgcgg cgaggtcgcc gcgatctgcc ggatcgggct
     6061 ggaccacgcg gagctggggt cgaccgtcga ggaggtcgcg gccgagaagg ccggcgtgat
     6121 caaggagggc gcgacggtcg tcgtcgagga gcagccggag gatgccctgc gggtgatcga
     6181 ggcgcgcgcg gccgaggtcg gcgccgaggt ccgcgtcgaa ggcgatgcct tcgccgtcga
     6241 ggaccgggag ctggccgtgg gtggccagcg cctcgcgatc cgctcgaccg ggcgctacga
     6301 ggatctcttc ctgccgctcc acggccccca ccaggcgcac aacgcggcgg tcgccgtcgc
     6361 cgcggccgag gcggtgctcg agtccgacct cgaggaagac gtgctgcggg aggcgctcgc
     6421 aggcgcgcgg tcgcccggac ggctcgaggt cgtctcctcc gatccactcg tcctcctcga
     6481 cggcgcgcac aaccccgatg gcgcggaggc gctcacgcgg gcgctcggcg aggggttcgt
     6541 ctggcgccgg ctccacctcg tgatcggggt gctgcgcgag aaggacctcg atgggatcct
     6601 cgagccgctg gcagcccggg tcgatgtcgc ctacgcgtgt tcgacctcca acccgcgggt
     6661 gcgcgacccg aaggacgtcg ccgccgcctg tctccgcgcc gggatccccg ccacggagca
     6721 tgcatccgtg gtcgatgcgc tcgacgcggc ccacgccgtc gccgcggaag acgacgtcat
     6781 cctggtcacg ggctcgctgt cgacggtcgg ggacgcgcgc ccgaggtacg ctccggcctg
     6841 acggctatac tgcggcgacc ccgaccggag gaaggatcgc ggatggccac cgaggagacc
     6901 ctcgtcatcg tcaagcccga cggcgtgcgc cgtcgcctcg tgggcgaggt gctcgcgcgg
     6961 atcgagcgca aggggctcac gatcgaggag ctccggatga tgcggatcga ccgcgagctc
     7021 gccgagcggc attacgggga gcatcgcgag aagcccttct tcggcgagct ggtggagttc
     7081 atcacctcgg ggaacgtcgt cgtcgcacgc gtctccggcg agagcgcgat cccggtcgtc
     7141 cggacgctga tgggcccgac gaacccggcc gaggccccac cgggcacgat ccgcggtgac
     7201 ttcggcacga tcatcaccga gaacctcgtg cacggcagcg actcgcccga gtcggccaag
     7261 cgcgagctcg ccctgttctt cgactgaccc gggtccgaag ggcatcgccc gatccctctg
     7321 gaggagcccg gaagatcgcc ctttccagga tcgcctgggt ttcctaggat tgtcggggtc
     7381 tgaaccggtc aggggttgag ggagggagag gggagatgtc tgtcacgctg acacgagcgc
     7441 tccgttggag cgtgctgatg atctcgatcg cggccgttgc cgccggaacg gttccggtcg
     7501 ccgcgtcccc cggcggggcg caggagctcc ccgtcggact gggcgtcagc gttggagatc
     7561 cccgcgaggt gggggcgaac gcttcggacg tcgaccgggt cgagagccac tcctccggcc
     7621 cacgctggta cgaggcgagc gagtgggcgg ggccgacgca gaactgcatc cacggctact
     7681 ccgagcagct ggggatggcg tggaacggct ggtacgggga gatcgggtcg agcccgacgg
     7741 tgggcaacgt gtactacgtc aaggtcgggt ggggggtctc gggcaacccg tgtggcggtg
     7801 gcgcgaaggt ccacgtggag at
//
LOCUS       DATLGG010000277         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_183914, whole genome shotgun sequence.
ACCESSION   DATLGG010000277 DATLGG010000000
VERSION     DATLGG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183914"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /gene="lepB"
                     /locus_tag="VM638_07895"
     CDS             complement(<1..589)
                     /gene="lepB"
                     /locus_tag="VM638_07895"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVM12380.1"
                     /translation="MARPGRHRAGAGSGSDDPPAPTDDAGEAPPADEEHHGPLSFLRE
                     LPGLILVAFLLALLIKTFLVQAFFIPSQSMEHTLEVGDRVLVNKIVYRFREPRRGDVL
                     VFENPGALQPDRNFAEDLVNWVTEGLGFSSDPQKDFIKRVVGLPGETIEVRRGRVFID
                     GKRIQEPYLHPERDIQDFAKTTIPPGRYFMMGDNRA"
     gene            complement(595..999)
                     /gene="rplS"
                     /locus_tag="VM638_07900"
     CDS             complement(595..999)
                     /gene="rplS"
                     /locus_tag="VM638_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HVM12381.1"
                     /translation="MNKTDLIETPRLRDDLPDFRPGDTVKVHVRVVEGNRERIQIFQG
                     VVIRRRGGGLRESFTARKVSFGVGVERTFPLHSPSVAKIEVVTRGDVRRAKLYYLRER
                     RGKRARIRELREPQRTTTAAGRSRTEATEDQG"
     gene            complement(1039..1722)
                     /gene="trmD"
                     /locus_tag="VM638_07905"
     CDS             complement(1039..1722)
                     /gene="trmD"
                     /locus_tag="VM638_07905"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVM12382.1"
                     /translation="MRVDVVTIFPGIFEGPLRESLLGKAIEAGTVQVRVHDLRAFAPD
                     RHRQVDDEPYGGGPGMVMMAEPIFRAVDSLDPAGARLILLSPAGRRLDHGLVRELAGE
                     QRLILLCGRYEGVDERVVEGLGAEEISIGDYVLSGGELPALVLIEAVTRLVPGVVGRR
                     ESVEQDSFSAGLLDHPHYTRPRELRGLAVPDVLVSGDHGQVAEWRRRAAEEKTRRNRP
                     DLLEPDPGA"
     gene            complement(1722..2288)
                     /gene="rimM"
                     /locus_tag="VM638_07910"
     CDS             complement(1722..2288)
                     /gene="rimM"
                     /locus_tag="VM638_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HVM12383.1"
                     /translation="MLRKRTWPPRSRSWSESLSSEELLAVGRIARAHGVRGEVSVQLL
                     TEVPERFEPGAELQLGPDGVRRVVVRSTRRHHGRLLIRFEGVEDRDAADALRGLLLLA
                     DAASSPAPPEGGFWVHEVVGLEVETEAGRSLGRVREVLHSPANDVWVVEGAGREVLIP
                     ALKDVVARVDLDARTAVVREVAGLFEEG"
     gene            complement(2242..2472)
                     /locus_tag="VM638_07915"
     CDS             complement(2242..2472)
                     /locus_tag="VM638_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVM12384.1"
                     /translation="MKELLEYIVRHLVEDPDGIEVTEIEDDRGVVLQLRVAPDDMGKV
                     IGRGGRMARAIRSIVRAAASQEDMAAKVEILE"
     gene            complement(2469..2891)
                     /gene="rpsP"
                     /locus_tag="VM638_07920"
     CDS             complement(2469..2891)
                     /gene="rpsP"
                     /locus_tag="VM638_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVM12385.1"
                     /translation="MRMGAKKRPFYRVVVADSRSPRDGRFIENIGKYHPLSDPSVIEI
                     DEERALHWLGKGAQPSQPVKVLMQKIGIWEKFRPDDRPRVRAEKAPKPAPAATADAGP
                     TEPEPAPAEEEAPPPEAEETAPAEGGDPEATEATSEEA"
BASE COUNT          408 a         1059 c         1010 g          414 t
ORIGIN      
        1 tcgcccggtt gtcgcccatc atgaagtagc gtccgggcgg gatcgtcgtc ttggcgaaat
       61 cctggatgtc gcgctcgggg tggaggtagg gctcctggat ccgcttcccg tcgatgaaga
      121 cgcggccccg ccggacctcg atcgtctctc cgggaagccc gaccacgcgc ttgatgaaat
      181 ccttctgagg atccgacgag aagccgagcc cctcggtcac ccagttgacg aggtcctcgg
      241 cgaagttgcg gtcaggctgg agcgcgccgg ggttctcgaa cacgagaacg tcgccgcggc
      301 ggggctcgcg gaaccggtag acgatcttgt tcaccaggac gcggtcgccg acctcgagcg
      361 tgtgctccat cgactgcgac gggatgaaga aggcctggac gaggaaggtc ttgatgagga
      421 gcgcgagcag gaacgccacg aggatgaggc cgggaagctc gcggaggaag gagagcgggc
      481 cgtggtgctc ctcgtccgcc ggtggagcct caccggcgtc gtccgttgga gcgggtgggt
      541 cgtcgctgcc ggaccccgcg cccgcgcggt gcctgccggg acgcgccatg ggcgctatcc
      601 ctggtcctcg gtggcctcgg tccggctccg acccgcagcg gtcgtcgttc gctgcggctc
      661 ccgcagctcg cggatgcgcg cgcgcttgcc ccggcgctcg cgcaggtagt acagcttcgc
      721 ccgtcggacg tcgccgcggg tgacgacctc gatcttcgcg acggagggcg agtgcagcgg
      781 gaacgtccgc tcgacgccga cgccgaagga gaccttccgc gcggtgaagc tctcgcggag
      841 cccgccccca cggcggcgga tcacgacccc ctggaagatc tggatccgct cgcggttccc
      901 ctcgacgacg cggacgtgca ccttgacggt gtcccccgga cggaaatccg gcaggtcgtc
      961 gcgcaggcgt ggcgtctcga tcaggtcggt cttgttcacg ggatcctcca ggaaaggagc
     1021 gccaatcata gcacctcgct acgcgcccgg atcgggctcc aggaggtcag gacggttgcg
     1081 ccgcgtcttc tcctcggccg cccgccggcg ccactccgcc acctgcccgt ggtcgccgga
     1141 caccaggacg tcggggacgg cgaggccgcg cagctcccgc ggacgcgtgt agtgggggtg
     1201 gtcgaggagc cctgccgaga aggaatcctg ctccaccgac tcgcgccgtc ccacgacccc
     1261 cgggacgagc cgggtgacgg cctcgatcag gacgagcgcg ggaagctcac cgcccgaaag
     1321 gacgtagtcg ccgatggaga tctcctcggc cccgagcccc tcgacgacgc gctcgtccac
     1381 cccctcgtac cggccgcaga ggaggatcag gcgctgctcg cccgccagct cgcggacgag
     1441 cccgtggtcg agtcgccgcc ccgccggcga gagcaggatc aggcgggcgc ccgccgggtc
     1501 cagcgagtcg accgcccgga agatcggctc ggccatcatc accatcccgg ggccgccacc
     1561 gtagggctcg tcgtcgacct gccggtgccg atccggtgcg aacgcgcgaa gatcgtggac
     1621 gcgcacctgg acggttccgg cttcgatcgc cttcccgagg aggctctcgc gcaggggccc
     1681 ttcgaagatc cccgggaaga tcgtcacgac gtcgacccgc atcatccctc ctcgaagagc
     1741 ccggcgacct cgcgcacgac cgcggtgcgc gcgtccaggt ccacgcgcgc cacgacatcc
     1801 ttcagcgccg ggatcaggac ctcgcggccc gccccctcca ccacccagac gtcgttggcc
     1861 ggcgagtgca ggacctcgcg gacgcgcccg agcgaccgac cggcttccgt ctcgacctcg
     1921 agcccaacca cctcgtgcac ccagaagccg ccctcgggtg gagccggaga gctcgcggcg
     1981 tcggcgagca ggaggaggcc gcggagggcg tccgccgcat cgcgatcctc gacgccctcg
     2041 aagcggatca gcagccgccc gtggtgccgg cgggtcgacc ggaccacgac gcgccggacc
     2101 ccgtcggggc ccagctggag ctccgctccc ggctcgaacc gctccggaac ctcggtgagg
     2161 agctgcacgg agacctcgcc gcgcacgccg tgggcgcgag cgatgcgccc gacggcgagc
     2221 agctcctcgg agctcagcga ctcactccag gatctcgacc ttggcggcca tgtcctcttg
     2281 cgaagcagcc gcgcggacga tcgagcggat cgcgcgggcc atccggccgc cccggccgat
     2341 gaccttgccc atgtcgtcgg gagcgacgcg gagctggagg acgacgccgc ggtcgtcctc
     2401 gatctccgtc acctcgatgc cgtcgggatc ctcgacgaga tggcggacga tgtactcgag
     2461 gagctccttc acgcctcctc cgacgtcgcc tccgtcgcct cggggtcacc gccctcggcg
     2521 ggggcggtct cctccgcctc ggggggcggc gcctcctcct cggcgggagc cggttcgggt
     2581 tccgtgggtc cggcgtcggc ggtcgccgcc ggggcgggct tcggggcctt ctcggcccgc
     2641 acccgcgggc ggtcgtcggg ccggaacttc tcccagatgc cgatcttctg catcagcacc
     2701 ttcaccggct gggacggctg agcgcccttg ccgagccagt ggagcgcccg ctcctcgtcg
     2761 atctcgatca ccgacgggtc cgacaagggg tggtacttgc cgatgttctc gatgaagcgc
     2821 ccatcgcgcg ggctgcgcga gtccgccacc acgacgcggt agaagggccg cttcttcgcg
     2881 cccatccgca t
//
LOCUS       DATLGG010000278         8663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_121664, whole genome shotgun sequence.
ACCESSION   DATLGG010000278 DATLGG010000000
VERSION     DATLGG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121664"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1292)
                     /locus_tag="VM638_07925"
     CDS             complement(18..1292)
                     /locus_tag="VM638_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12386.1"
                     /translation="MLRRLLRPPAPGFGNEDLPDVAASQWWSRDRILDYQAERLRRLV
                     AYVWEEVPFYRDLMREHRVRPADVQGPDDLHALPVVEKSVLHGHAERITRQEAVHEYA
                     LTGGTSGFRLRWARNRRWLELFTACLYRGFGWAGLTKETKVVSFYSRNIGTVSERALI
                     LRESLEAGRVETELEEVRAFGPEAAYCYASSAFMVASYLLDTGQTLPLRTVVTTGDQL
                     LPEYVPVIERAFGCRVWDNFGCNDGGVWGAQCEERSGFHHDAERSILEVVDGRLVATD
                     LWNSAFPFLRYAIGDAGAWLEGDCPCGRGSPRFTAQGRLYDVIVGPTAALAPPAATAM
                     FRVDGLADFRIVQTDPRTVEVLVVRASGFDAGGFDRGIQALREALPGMEVVPRFVETI
                     APTTSGKRRIVENRTGLSPASVWGGPTPADGP"
     gene            1344..2759
                     /locus_tag="VM638_07930"
     CDS             1344..2759
                     /locus_tag="VM638_07930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HVM12387.1"
                     /translation="MQGSPSRPARVGIAGTFDVDNFGDVLFPHVARNELERRLPGVEI
                     RSFSPLGRASAWDRGATSEALGPWSEERLDELRDSLDCLLIGGGDIIHTRFERIADCY
                     GIGEDEIRALQLDRFFIDGLGTEREAARPVVWNGVGVPFDLSPEEEARVRKALDGRPY
                     VAVRDRGSAARLEAVGAGGVEVVPDTAILVPRLVGAEVRGKRLRQLRAMGWWPEDGRI
                     LLIQGNAALEPFLDGLADGVRRILEARPDLTPVTISLGPCHGDDRFAAALAQRLGRPV
                     PHVPGEGSLEDMVAAIAAATGFVGSSLHGNITAFAHDVPGVILDMNQHAKHRGFAEMT
                     GDLGMLVSDPAELAEAFLAQVRRDRTGDIERLQMRADDHFDRIARVVEAAVAPDAEPA
                     RVSSEERLVELERELETLRRAYEARSRRLASDAAAYADQIAAQRIPMRKAEEFLRSRT
                     YRYVSWLRAVYLGVRRPFGRR"
     gene            2756..3952
                     /locus_tag="VM638_07935"
     CDS             2756..3952
                     /locus_tag="VM638_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVM12388.1"
                     /translation="MTVAVFEPRERPVLSVVMVTYGGGEWAERALDALRARTAVPFEV
                     VIVDNASPDGTADRLAAGVSGATLIRNDRNVGYAAAANLGAARARAPFVCFLNPDALV
                     EEGWDRTILDAFDDPTVGAAVPRLLDLDGRLQEAGSLVGRDGYTWALGHDDGDPADPA
                     FAFPLEVDYGSACLVVRRTAFSEVGGFDPAFFPAYGEDVDLCLSLQERGWRVVHEPRA
                     RVRHVRSVSLGTGGAERWIHRSRARLVRRWGAQMRERPWLAEPAFFPHRVAAVRDHRA
                     VDRVLVVGDRLADLRDVEEHLFALAARRDARVTTVLAAETDAHAARELAVRGVEVVLG
                     PKGGPDWFEARRYQASAVVVGTRRFDRELDATQPQAPRVALESLSRDPAGALADAGVA
                     TAPGGT"
     gene            3949..5907
                     /locus_tag="VM638_07940"
     CDS             3949..5907
                     /locus_tag="VM638_07940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12389.1"
                     /translation="MSEDERRRLEAWQRELEAREMRVQLREAAMRKRQLDRYGAPSGS
                     EERLFDLERRVWELGRFRETVTESAWYPLIQGVRRFADGLLPWGTRRRSFFLAPVRAV
                     RMLAHDPGGFVRRIPKARLWAPGLIRPADPPPHTEERYRVWHRRRYARARLRAAAAEA
                     ADLRDGPLISVVMPTYNAEPAWLRTAIGSVRAQLYPRWELCIADDASTRTEAHAVLRR
                     AAADDPRIKVRFLEENRGISGASNAALELATGEFVALLDHDDELKPDSLLEVAKLIRG
                     EPDLDYVYTDEDKRSPDDRLIDAFFKPDWSPDLLMSMNYLSHLSVFRRAVLERVGGFR
                     SEMDGSQDYDLVLRVTEATDRIGHVPLPLYTWRMVPGSAAHSDEAKPWAYTAARRALE
                     DALRRRGIAGDVEHGPFLGAYRVRYAIDGDPRVAVLVRDPGDEDLTRRTVEGIEREST
                     YENRLVEVVPPGPFAERVNDAVGGVDADLLVVVEAGMEVLASGWIEALVRHGQRKGVG
                     VVGGRILGPLGIPLHEGAAVVGGEVLEAAGVMQFTLGFVTRNCSAVGGGLLMIRPGLI
                     RELGGFDPAFDVELGPIDLSLRAREEGHLVVYTPDATLGWLHGPRAEPSRAERERFRR
                     RWAGYRDPYYSPNLDPDEAYAVRLRTHG"
     gene            5900..7495
                     /locus_tag="VM638_07945"
     CDS             5900..7495
                     /locus_tag="VM638_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12390.1"
                     /translation="MAEPWTTRSWRGFPLALWLIAVLHALTLFVYSVLFPMYRAPDEH
                     IHVDRVLAFRDSFVFHEFHEREIDAVVDASRASVAGAGGSRHLRAEDAPPRHERPPFE
                     DVPPSPRQRHPNIVSNHPPLYYAYLTTASTMLTSLVPGIHWSWDLVVALLRLLTILLL
                     VPLPFLLFATARRLRAPPEASLAAALVPLGIPMLTNIGSTVNNDNLLLALGGVLAYLV
                     ARIGTGDASVRTAVVAGVVGGLALYTKGFALTVPLWLVAGYAALARRAADVALAGRRL
                     AVSLGLAFAAGGWWWLRNVLLFDRVQTGVGGLRAPAPPEFVPDWGWWLGKYASWLLDR
                     FWGSFGRVDVNLPRAISLAATALVLLGVAWALVSRPRERGDRWTLAALLVPFLSLLAF
                     VTYGATRAYRYAGQPVGFHGRYLYGGVPGLAVVAAVGWTVLLGRLRRLVPALAFAWVA
                     VMQLVSVPMILRWYWGPAGPAPITERVAAVLAWSPWPPAVVVTAIVLTAAVGATTAVV
                     LVRHALAPSSHSASGPDTSARRP"
     gene            complement(7446..8393)
                     /locus_tag="VM638_07950"
     CDS             complement(7446..8393)
                     /locus_tag="VM638_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVM12391.1"
                     /translation="MGTPAEQPERDGPKVLYVIGKGRSGSTLLDLILGELDGYVSTGQ
                     LDRIWTSGLIKGYDCGCGLPLPKCEVWTPILEDAFGGDVDPHAIARTQAEVLAWKHAP
                     RVLRHAGRGPAGWPALERYVAIMSRLYAAIATITGARVIVDSSKAPAHPGSVGLVPGI
                     TPYVVHLVRDPRAVTYSWKRRKALQGRGEMKEMPRFGPVYSSVSWVARNVAARRNRRV
                     LGRDRSIVLRYEDLAERPREAVERIARMVGEVPETLPFTDERTVELSPNHTVSGNPIR
                     TNVGSIELRRDDEWRRRISTTDRVTASVLTYPVRKRYGY"
     gene            8416..>8663
                     /locus_tag="VM638_07955"
     CDS             8416..>8663
                     /locus_tag="VM638_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12392.1"
                     /translation="MADDSLLIEGVDVAGPRLRIRAGRAFADAFLRDDLWAEYEGISL
                     EQVPRWVLELPFLLNVAPVVWLSGRRFTVPELDEELASS"
BASE COUNT         1072 a         3106 c         3197 g         1288 t
ORIGIN      
        1 ccgttgggct ggccgcttca cgggccgtcg gcgggtgtcg gcccgcccca cacgctcgcg
       61 ggggacaggc cggtccggtt ctcgacgatc cgccgtttcc ccgacgtcgt gggcgcgatc
      121 gtctcgacga accgcggcac cacctccatc ccgggcaggg cctcccgcag cgcctggatc
      181 ccgcgatcga acccgcccgc gtcgaagccc gaggcacgga ccacgagcac ctcgaccgtc
      241 cgcgggtccg tctgcacgat ccggaagtcc gcgagaccgt ccacgcggaa catcgcggtc
      301 gcggccgggg gagcgagggc cgcggtcggg ccgacgatca cgtcgtacag ccgtccctgg
      361 gccgtgaacc gaggggagcc tcggccgcag gggcagtcgc cctcgagcca cgcgccggcg
      421 tccccgatcg catagcgcag gaacgggaac gcgctgttcc acaggtcggt ggcgacgagc
      481 cggccgtcga cgacctcgag gatgctgcgc tcggcatcgt ggtggaagcc cgagcgctcc
      541 tcgcactgcg cgccccagac cccgccgtcg ttgcagccga agttgtccca gacgcggcac
      601 ccgaaggctc gctcgatgac cggcacgtac tcgggaagca gctggtcccc ggtcgtgacc
      661 acggtgcgga gggggagcgt ctggccggtg tcgagcaggt acgaggcgac catgaacgcc
      721 gaggaggcgt agcagtacgc ggcctccggt ccgaacgcgc ggacctcctc cagctcggtc
      781 tcgacccgcc cggcttcgag cgactcgcgg aggatcagcg cacgctcgct caccgttccg
      841 atgttccgcg agtagaagct gacgaccttc gtctccttcg tgagacccgc ccagccgaac
      901 ccccggtaga ggcacgcggt gaagagctcg agccagcgcc ggttccgcgc ccagcggagc
      961 cggaacccgc tcgttccgcc cgtgagcgcg tactcgtgca ccgcctcctg gcgggtgatg
     1021 cgttccgcgt gaccgtggag gaccgacttc tcgaccaccg gaagggcgtg gaggtcgtcg
     1081 gggccctgga cgtcggcggg ccgaacccgg tgttcccgca tcaggtcgcg gtagaacggc
     1141 acctcctccc agacgtaggc gacgaggcgt cggagacgct cggcctggta gtcgaggatg
     1201 cggtcgcggc tccaccactg cgatgcggcg acgtcgggga ggtcctcgtt gccgaacccc
     1261 ggggcggggg ggcgcaggag tcggcggagc atcgggccat gatagcggcg ggcgttccgg
     1321 gagcccctat atcatcccgt tccgtgcagg gctccccgtc ccggccggcg cgcgtcggca
     1381 tcgccggcac cttcgacgtc gacaacttcg gtgacgtcct cttcccccac gtggcgcgca
     1441 acgagctgga gcggcggttg ccgggcgtcg agatccggtc gttctccccg ctcggccgtg
     1501 cgagcgcctg ggaccgcggc gcgacctccg aggcgctcgg tccctggtcc gaggaacggc
     1561 tggacgagct gcgggattcg ctcgactgcc tcctgatcgg tggcggcgac atcatccaca
     1621 cgcgcttcga gcggatcgcc gactgctacg gcatcggcga ggacgagatc cgggcgctgc
     1681 agctcgaccg cttcttcatc gacggcctcg ggacggagcg cgaagcggcg cgcccggtcg
     1741 tctggaacgg cgtcggggtc ccgttcgacc tctcgcccga agaggaggcc cgcgtgcgca
     1801 aggcgctcga cggccgcccc tacgttgcgg tccgcgatcg cggctccgcg gcgaggctcg
     1861 aggcggttgg cgccggcggc gtcgaggtcg tgccggatac cgccatcctc gtgccgcggc
     1921 tcgtcggcgc ggaggtccgg ggcaagcgcc tccgccagct ccgggcgatg gggtggtggc
     1981 cggaggacgg gcggatcctg ctgatccagg ggaacgccgc gctcgagccg ttcctcgacg
     2041 gccttgccga cggcgtccgt cggatcctcg aggcgcgacc cgacctgacg ccggtcacga
     2101 tctcgctcgg cccctgtcac ggggacgacc ggttcgccgc ggcgctcgcc cagcggctcg
     2161 ggcggcccgt cccgcacgtc cccggagagg gatcgctcga ggacatggtc gcggcgatcg
     2221 cggcggcgac ggggttcgtc gggagctccc tgcacggcaa catcaccgcc ttcgcgcacg
     2281 acgtcccggg cgtgatcctc gacatgaacc agcacgcgaa gcaccgtggc ttcgccgaga
     2341 tgaccggtga cctcgggatg ctcgtctccg acccggccga gctggcggag gcgttcctcg
     2401 cgcaggtccg ccgcgatcgc accggcgaca tcgagcggct gcagatgcgg gccgacgatc
     2461 acttcgatcg gatcgcgcgc gtcgtcgagg cagcggtcgc gcccgacgcc gagccggctc
     2521 gcgtcagttc cgaggagcgc ctcgtcgagc tcgagcgcga gctcgagacg ctccggcggg
     2581 cgtacgaggc ccggagccgt cgcctcgcga gcgacgcggc cgcctatgcg gaccagatcg
     2641 cggcgcagcg catcccgatg cgcaaggcgg aggaattcct ccgctcgcgc acgtaccgct
     2701 acgtctcgtg gctccgcgcg gtctacctcg gggtgcgtcg gcccttcggg aggcgatgac
     2761 ggtggccgtc ttcgagccga gggagcgacc ggtgctgtcg gtcgtgatgg tcacctacgg
     2821 tggcggcgag tgggcggagc gcgcgctcga tgcgctccgc gctcggacgg ccgtgccgtt
     2881 cgaggtcgtg atcgtggaca acgcctcacc cgacgggacg gcggaccgac tcgcggctgg
     2941 cgtctccgga gcgaccctga tccgcaacga ccgcaacgtg ggctacgcgg ccgccgcgaa
     3001 cctcggcgcg gcgcgggcac gagctccgtt cgtctgcttc ctcaacccgg acgcgctcgt
     3061 cgaggagggc tgggaccgga cgatcctcga tgcgttcgat gacccgacgg tcggcgccgc
     3121 cgtcccgcgt ctgctcgacc tcgacggccg gctccaggaa gccggctccc tcgtggggcg
     3181 tgacggctac acgtgggcgc tcggccacga tgacggcgat ccggccgacc ccgccttcgc
     3241 gttccccctc gaggtcgatt acggctccgc gtgcctggtc gtgcgccgga ccgcgttctc
     3301 ggaggtcggg gggttcgatc ccgcgttctt ccccgcgtac ggggaggatg tggacctctg
     3361 cctctcgctc caggagcgag gttggcgggt cgtgcacgag ccgcgggcga gggtccgcca
     3421 cgtccggtcg gtctcgctgg gtacgggcgg cgctgaacga tggatccacc gcagccgggc
     3481 ccggctcgtg cggcggtggg gtgcgcagat gcgtgagcgg ccgtggctgg cggagccggc
     3541 gttcttcccg caccgcgtcg cggcggtccg ggaccatcgc gcggtggacc gggtgctggt
     3601 ggtcggggac cggctcgcgg atctgcgtga tgtcgaggag cacctgttcg cgctcgcggc
     3661 gcggcgcgat gcgagggtga cgaccgttct ggcggccgag accgacgccc atgccgcgcg
     3721 cgaactcgcc gtccgagggg tcgaggtcgt gttgggtccg aagggggggc cggactggtt
     3781 cgaggcgcgc cggtaccagg cgtcggcggt cgtcgtcggc acgaggcggt tcgaccggga
     3841 gctggacgcg acccagccgc aggccccgcg cgtcgctctc gagagcctga gccgcgatcc
     3901 ggccggggcc ctcgccgacg ccggagtggc cacggcgccg ggtgggacat gagcgaggac
     3961 gagcggcgcc gcctcgaagc gtggcagcgg gagctcgagg cccgggagat gcgggtccag
     4021 ctccgcgagg ccgccatgcg caagcgtcag ctcgaccgct acggcgcgcc gtccggcagc
     4081 gaggaacggc tcttcgacct cgagcggcgc gtctgggagc tcggtcggtt ccgcgagacc
     4141 gtgacggaga gcgcctggta cccgctcatc cagggggtgc gtcgattcgc ggacgggctg
     4201 ctgccctggg gcacgcggcg ccggagcttc ttcctcgccc cggtccgcgc cgtgcggatg
     4261 ctcgcgcacg atcccggcgg gttcgtacga cgcatcccga aggcgaggct ctgggcgccc
     4321 gggctgatcc gacccgccga tccgccaccg cacaccgagg agcgctaccg ggtctggcac
     4381 cgacgccggt acgcacgcgc ccggctgcgc gcggcggcgg ccgaagccgc ggatctgcgg
     4441 gacggaccgc tgatcagcgt cgtgatgccc acctacaacg ccgagccggc ctggctgcgg
     4501 acggcgatcg gctcggtccg agcgcagctg tacccccggt gggagctgtg catcgccgac
     4561 gacgcctcca cccggaccga agcgcacgcg gtcctgcgcc gtgcggccgc ggacgacccc
     4621 aggatcaagg tgcgcttcct ggaggagaac cgcgggatct ccggcgcctc gaacgccgcg
     4681 ctcgagctcg cgacgggcga gttcgtcgcg ctcctcgacc acgacgacga gctgaagccg
     4741 gactccctcc tggaggtcgc gaagctcatc cgcggggagc ccgacctcga ctacgtctac
     4801 accgacgagg acaagcgctc gccggacgac cgcctgatcg acgcgttctt caagcccgac
     4861 tggtcgccgg acctgctgat gtccatgaac tatctcagcc acctgtcggt gttccgtcgg
     4921 gccgtgctcg agcgcgtcgg tgggttccgc tccgagatgg acgggagcca ggactacgac
     4981 ctcgtgctgc gggtgacgga ggcgaccgat cggatcggcc acgtgccgct cccgctctac
     5041 acctggcgca tggtgccggg ctccgccgcg cactcggacg aggcgaagcc ctgggcctac
     5101 acggcggcgc gacgtgcgct cgaggatgcc ctccgccgtc gcgggatcgc cggcgacgtc
     5161 gagcacgggc cgttcctcgg cgcctaccgc gtgcgctacg cgatcgacgg tgatccgcgc
     5221 gtggctgtcc tcgtgcgcga cccgggggac gaggatctga cgcgacgaac ggtcgagggc
     5281 atcgagcgag agagcaccta cgagaaccgt ctcgtcgagg tggttccgcc gggaccgttc
     5341 gccgagcgcg tgaacgacgc cgtcggaggg gtggatgcgg atctgctcgt ggtggtggag
     5401 gccgggatgg aggtgctggc ctccggatgg atcgaggcgc tcgtgcggca cggccagcgg
     5461 aagggggtcg gggtcgtcgg cggccggatc ctcggtccgc tcgggatccc gctgcacgag
     5521 ggggccgcgg tcgtcggcgg ggaggtgctc gaggcggccg gggtcatgca gttcacgctc
     5581 gggttcgtga cccggaactg ctcggcggtc gggggcggtc tgctgatgat ccgcccgggg
     5641 ctgatccgcg agctcggcgg gttcgacccc gccttcgatg tcgagctcgg acccatcgac
     5701 ctgtcgctgc gagcccgcga ggagggccac ctggtcgtct acacgccgga cgcgaccctc
     5761 ggctggctgc acgggcctcg cgccgagccc tcgagggctg agcgcgagcg attccgccgc
     5821 cggtgggccg ggtaccgcga cccgtactac agcccgaacc tcgacccgga cgaggcgtac
     5881 gccgtccggc tgcggacgca tggctgagcc ctggacgacc cggtcgtggc gggggttccc
     5941 gctcgcgctc tggctgatcg ccgtgctgca cgcgctcacg ctcttcgtct actcggtgct
     6001 gttcccgatg taccgggcgc ccgacgagca catccacgtc gaccgtgtcc tcgcgttccg
     6061 ggattccttc gtcttccatg agttccacga gcgggagatc gacgccgtgg tcgacgcttc
     6121 ccgcgcgagc gtcgccggtg cgggcgggtc gcggcacctg cgcgccgagg acgcgccccc
     6181 gcggcacgag cgcccgccgt tcgaggacgt cccaccgagc ccgcgccaac ggcacccgaa
     6241 catcgtgtcg aaccacccgc cgctctacta cgcgtacctc acgaccgcgt cgacgatgct
     6301 cacctcgctc gtcccgggga tccactggtc gtgggacctc gtcgtggcgc tcctgcggct
     6361 cctgacgatc ctgctgctcg tcccgctgcc gttcttgctg ttcgcgacgg cccggcgcct
     6421 ccgtgcgccg ccggaggcct cgctcgcggc ggcgctcgtg cccctgggga tcccgatgct
     6481 cacgaacatc ggctcgaccg tgaacaacga caacctcctg ctggcgctcg gcggggtcct
     6541 cgcctacctg gtcgcgcgga tcgggacggg cgacgcctcg gtgcggaccg cggtcgtcgc
     6601 gggcgtggtg gggggcctcg cgctctacac gaaggggttc gcgctcacgg tgccgctctg
     6661 gctcgtcgcc ggctatgcgg cgctcgctcg acgggccgcc gacgtcgccc tggcggggcg
     6721 ccgcctcgcc gtctcgctcg gcctcgcgtt cgcggcgggt gggtggtggt ggctccgcaa
     6781 cgtcttgctg ttcgaccgcg tccagaccgg cgtcggaggc ctccgcgcac cggcgccccc
     6841 cgagttcgtt ccggactggg gatggtggct cgggaagtac gcgtcgtggc tgctcgaccg
     6901 gttctggggc tccttcggcc gggtcgacgt gaacctcccg agggcgatct cgctggcggc
     6961 gaccgcgctc gtgctcctgg gtgtcgcctg ggcgctcgtc tcgcgtccgc gggagcgggg
     7021 cgaccgctgg acgctggcag cgctgctcgt gccgttcctc tcgctgctgg ccttcgtgac
     7081 gtacggggcg acccgcgcgt accgctacgc ggggcagccc gtcgggttcc acgggcgcta
     7141 cctctacggg ggggtcccgg ggctcgcggt cgtcgcggcg gtgggctgga ccgtcctcct
     7201 cgggcgcctg cggcggctcg tgcctgccct cgcgttcgcc tgggtcgcgg tcatgcagct
     7261 cgtctccgtc ccgatgatcc tgcgctggta ctggggtccc gcggggccgg cgccgatcac
     7321 cgagcgggtg gcggcggtgc tggcgtggtc accgtggccg ccggcggtgg tcgtgacggc
     7381 gatcgtcctg acggcggcgg tcggggccac gaccgcggtc gtgctcgtaa ggcacgcgct
     7441 cgcgcctagt agccatagcg cttccggacc ggatacgtca gcacggaggc cgtgacccga
     7501 tcggtcgtcg agatccgccg gcgccactcg tcgtcacggc ggagctcgat cgacccgacg
     7561 ttcgtgcgga tcgggttccc cgagacggtg tggttcgggg agagctcgac ggttcgctcg
     7621 tcggtgaagg ggagcgtctc cgggacctcg ccgaccatcc tcgcgatccg ctcgaccgcc
     7681 tcgcgcggcc gctcggcgag atcctcgtac cgcaacacga tcgagcgatc gcggcccagg
     7741 acgcgtcggt tccgtcgagc ggcgacgttg cgcgccaccc agctcaccga gctgtacacc
     7801 ggaccgaacc gcggcatctc cttcatctcg ccgcggccct ggagggcctt gcggcgcttc
     7861 cacgagtacg tcaccgcccg cgggtcgcgg acgaggtgca cgacgtaggg cgtgatcccc
     7921 ggcacgagcc cgaccgagcc gggatgcgcg ggagccttcg aggagtcgac gatcacgcgc
     7981 gccccggtga tcgtcgcgat cgccgcgtac aggcgcgaca tgatcgcgac gtagcgctcg
     8041 agcgccggcc acccggccgg cccgcggccc gcgtgccgca ggacgcgcgg cgcgtgcttc
     8101 cacgcgagca cctcggcctg cgtgcgcgcg atcgcgtgag gatcgacgtc accaccgaac
     8161 gcgtcctcga ggatcggcgt ccacacctcg cacttcggca gcggcaggcc gcagccgcag
     8221 tcgtagccct tgatgagccc tgaggtccag atgcggtcga gctggccggt cgagacgtag
     8281 ccgtcgagct cgccgaggat caggtcgagg agcgtgctcc cgctccgtcc cttcccgatc
     8341 acgtagagga ccttcgggcc gtcgcgttcc ggctgctcgg cgggggttcc cacggctcgg
     8401 atagtatcgg tcgccgtggc cgacgacagc ctcctgatcg agggcgtcga cgtcgccggg
     8461 ccccgcctcc gcatccgagc aggccgagcc ttcgccgacg ccttcctgcg cgacgatctc
     8521 tgggcggagt acgagggcat ctcgctcgag caggtcccgc ggtgggtgct ggagctgccc
     8581 ttcctgctga acgtcgcccc ggtcgtgtgg ctgagcggcc ggcgcttcac cgtccccgag
     8641 ctcgacgagg agctggcgtc ctc
//
LOCUS       DATLGG010000279         5139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_11054, whole genome shotgun sequence.
ACCESSION   DATLGG010000279 DATLGG010000000
VERSION     DATLGG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11054"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="cofD"
                     /locus_tag="VM638_07960"
     CDS             <1..864
                     /gene="cofD"
                     /locus_tag="VM638_07960"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HVM12393.1"
                     /translation="ITVIVNTGDDLVLHGLHVSPDLDSVTYWLADVADRERGWGRRDE
                     TFRAWEEISRLGGVAWFRLGDLDLATHLVRTDILRGGGTLSEATRRITDAFGIEAQVL
                     PMTDDAVTTRVEVATEGDPLDLHFQEYWVARGARDEVVAVRYEGATAARAVPAALEAL
                     ARADAIVVCPSNPVASIQPILAVADLRAAVVARRDRAVAVTPIVGGAPLRGMADRLLP
                     AVGHEVSATGVARMYRDICSAFVVDRRDEAESMRIREETGLRVAVTETVMTDDAAAAD
                     LARVAIEAVAG"
     gene            861..2162
                     /locus_tag="VM638_07965"
     CDS             861..2162
                     /locus_tag="VM638_07965"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HVM12394.1"
                     /translation="MTLEVLPVSGLPEIRPGDDLADLIVAAVGADGLGDGDVVAVAQK
                     AVSKAEGRLVPEGEGKVAWVAREASRIVARRGDLVIAETRHGFVCANAGIDVSNVPRG
                     SLSLLPEDPDASAARIAAGISERTGRKVGVVVTDTFGRPWRRGLVDVAIGCAGLPALL
                     DLRGEPDREGRLLESTVMAIADQAAAAAGIVMAKAGGVPVAVVRGLPRPSDAPAGAAR
                     DLVRPATEDLFRSAPLYAISSRRTVRAFRDEAVPDDAVERAVAAALTAPVPHGSRHRA
                     PPWRWTILRRGERRRRLLAAMADAWRRDLRADGFPEERIERRIERSDAILAAAPTLAI
                     PAVSLEGADAYPDARRREAERDMFLLATGAAVQSFMLALHAQGVGSSWISSTLFCKEE
                     AATALGLDAGWLPMGAVAAGFPAEPPPERPPVDPSPAITWL"
     gene            complement(2149..2442)
                     /locus_tag="VM638_07970"
     CDS             complement(2149..2442)
                     /locus_tag="VM638_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12395.1"
                     /translation="MPSPSASRAPAAIRAVAAGFPVLVVLAGWAVVSAIGGRSLPWFV
                     LLAAGGALFLAAAISAACAAHAVVRRRLGWALALVVGWPVLVPVYLLRTVRAR"
     gene            2466..3224
                     /locus_tag="VM638_07975"
     CDS             2466..3224
                     /locus_tag="VM638_07975"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HVM12396.1"
                     /translation="MAKKKRKKKPTTPRPAAQPVSQAADAQGRQERKEAARREREERI
                     KRARRRQRLRRLVRWVAILAVIALIVGFIWWRGAAGRRVLQAADAAAERIGCEAIVER
                     DEKGRSHEPPYEYTERPATSGSHAPSTLDPAVSVYDDPIDVPAGDQGVSLEPQAVHNL
                     EHAYVIMYYRSEGPQALPDAVVQRLTQLAEREEKVILAPYSQLPQGTSLTLAAWTRVQ
                     QCPAVRDAGAAEQVARGFIQRFRGGGDAPEPTAP"
     gene            complement(3226..4731)
                     /locus_tag="VM638_07980"
     CDS             complement(3226..4731)
                     /locus_tag="VM638_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVM12397.1"
                     /translation="MRARLVGIMVVVALFPLGGSASANTTFTFHGSGWGHGIGLSQYG
                     AFGLAQEGWSANRILKHYYSGTSVAEMSPPAKHFRIGLVQSRSAVTIEAVGGRMRLTL
                     TSGKVIETVPSGGRRRIERTDGRFRVGAPGTTGRLWGSSGDALRVTRLDGGSIHLAEW
                     GHTLNRGRLELAGASSSRLHAVAIIGPQAYLYGIAEVPSSWPGATLQAQAIAARTYAF
                     RKVASTPGQRRSDCACALVASTRDQYFIGREKELGPMGDRWVAAVRDTVGRVVRHNGN
                     LITTYYSSSSGGFTENVENVWSGAAATPYLKAVCDPGDFLASNPNRTWSADFSDATLT
                     SRLRSLTGSIGTITRFTEYRLGISGRVKTVKVVGTSGSKVVKGWDLRSALGLKDTRFR
                     VGKNLNVVGKIREAYDEQMCAPGRATSAQRSIAGGRFQEFQRGRMYLNDATAAVTWLR
                     GPVLDAYLARDAHAGSLGLPRSLTRLADGGWRGRFDGGTIECSAGGTCSVS"
     gene            complement(4785..5027)
                     /locus_tag="VM638_07985"
     CDS             complement(4785..5027)
                     /locus_tag="VM638_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12398.1"
                     /translation="MGDLVVIGPRCVIAAGARIDGSVLHEGVTVGSGAIVEGSVVGAG
                     AVIGPGATLRGSVLGAGARVEPGTVVEEARLSGASG"
BASE COUNT          693 a         1862 c         1912 g          672 t
ORIGIN      
        1 atcacggtga tcgtcaacac cggcgacgac ctcgtcctgc acgggctgca cgtctcgccc
       61 gacctcgact ccgtcaccta ttggctggcg gacgtcgccg accgggagcg cgggtggggc
      121 cggcgtgacg agacgttccg cgcgtgggag gagatcagcc gtctcggtgg ggtggcgtgg
      181 ttccggctcg gggacctcga cctcgcgacg cacctcgtgc gcaccgacat cctgcggggc
      241 ggaggaacgc tgtcggaggc gacccggcgg atcacggacg cgttcggcat cgaggcgcag
      301 gtcctgccga tgaccgacga tgccgtgacc acccgcgtcg aggtcgcgac cgaaggcgac
      361 cccctcgacc tccacttcca ggagtactgg gtggcgcggg gcgcgcgcga cgaggtcgtg
      421 gcggtgcgct acgagggtgc gacggccgcc cgcgcggtcc ccgcggcgct cgaggcgctg
      481 gcgcgcgccg acgccatcgt cgtctgtccc tcgaacccgg ttgcgagcat ccagccgatc
      541 cttgccgtcg ccgacctccg ggcggccgtg gtggcgcggc gggaccgggc cgtggcagtg
      601 acgccgatcg tcggaggcgc gccgctgcgg gggatggcgg accgtctgct gccggccgtc
      661 ggccacgagg tgtccgcgac cggggtcgcg cggatgtacc gcgacatctg ctccgcgttc
      721 gtggtcgatc gacgggacga ggcggagtcg atgcggatcc gggaggagac ggggctccgc
      781 gtcgccgtga cggagacggt gatgaccgac gacgcggcgg ccgcggacct ggcgcgcgtc
      841 gcgatcgagg cggtggcggg atgaccctcg aggtcctgcc ggtctccggg ctccccgaga
      901 tccgtcccgg agacgacctc gcggacctga tcgtcgctgc cgtcggggcc gatggactcg
      961 gcgacgggga cgtcgtcgcc gtcgcccaga aggcggtctc gaaggcggag gggcgcctgg
     1021 tccccgaggg tgaggggaag gtcgcatggg tcgcccgaga agcgtcgcgg atcgtcgcgc
     1081 ggcgcgggga cctcgtgatc gccgagacgc gccacgggtt cgtctgcgcg aacgcgggca
     1141 tcgacgtctc gaacgttccg cgcggctccc tctcgctcct ccccgaggat cccgacgcct
     1201 cggccgcgcg gatcgccgcc gggatcagcg agcgcaccgg gaggaaggtc ggcgtggtcg
     1261 tcaccgacac gttcgggcgc ccttggcggc gcggtctcgt ggacgtcgcg atcggctgcg
     1321 cgggtctccc ggcgctcctc gacctccggg gcgagccgga ccgggagggc cggctgctcg
     1381 agagcacggt gatggccatc gcggaccagg ccgcggccgc agcggggatc gtgatggcga
     1441 aagccggcgg tgtccccgtg gccgtcgtgc gaggtctccc tcggccgtcc gacgcgccgg
     1501 ccggagcggc ccgggatctc gtccggcccg ccacggagga cctcttccgc tccgctccgc
     1561 tgtacgcgat ctcgtcccgt cgcacggtgc gggccttccg cgacgaggcc gtcccggacg
     1621 atgcggtcga gcgtgcggtg gcggcggcgc tgaccgcccc cgtcccccac ggatcccgcc
     1681 atcgcgcgcc gccgtggcgc tggacgatcc tgcgtcgggg cgagcgacgg cgccggctcc
     1741 tcgccgcgat ggcggatgcg tggcgacggg acctgcgtgc cgatggtttc ccggaggagc
     1801 ggatcgagcg ccggatcgag cggtcggacg cgatcctcgc cgccgcgccg accctcgcga
     1861 tcccggcggt gtcgctcgag ggcgcggacg cctaccccga cgcccggcgc cgcgaggccg
     1921 agcgcgacat gttcctgctc gcgacgggcg cggccgtcca gtcgttcatg ctcgccctcc
     1981 acgcgcaggg ggtcggctcg agctggatct cctcgaccct cttctgcaag gaggaggccg
     2041 cgacggcgct cgggctcgac gcgggctggc tgccgatggg tgccgtcgcg gccggcttcc
     2101 cggcggagcc tccgcccgag cggccgccgg tcgacccctc cccggcgatc acctggctct
     2161 gacggtccgc aggagataca ccggcacgag gaccggccat cccaccacga gcgccagcgc
     2221 ccagccgaga cgtcgccgga cgacggcgtg cgcggcgcac gccgctgaga tcgcggccgc
     2281 gaggaagagc gcgccccccg ctgccaggag cacgaaccat gggagcgacc ggccgccgat
     2341 cgcggacacc accgcccacc cggccagcac caccagcacc gggaacccgg cggcgaccgc
     2401 gcggatcgcg gccggtgctc ggcttgcgga gggcgagggc atcccggcta tagttactcg
     2461 ttcccatggc gaagaagaag cgcaagaaga agccgaccac gcccaggccc gcggcgcagc
     2521 cggtctccca ggctgccgac gcgcaggggc gccaggagcg caaggaggcc gcccgccgcg
     2581 agcgcgagga acggatcaag cgcgcgcgcc ggcgtcagcg cctgcgtcgc ctggtccggt
     2641 gggtcgccat cctcgccgtc atcgcgttga tcgtgggctt catctggtgg cgcggcgcgg
     2701 ctgggcgacg ggtcctgcag gccgccgacg ccgccgccga gcgcatcggc tgcgaggcga
     2761 tcgtcgagcg cgatgagaag ggccggagcc acgagccgcc ctacgagtac accgagcgcc
     2821 cggcgacctc cgggtcccac gccccgtcga cgctcgaccc cgccgtctcg gtctacgacg
     2881 acccgatcga cgtgccggcg ggcgatcagg gcgtctcgct cgagccccag gccgtgcaca
     2941 acctcgagca cgcctacgtg atcatgtact accgctcaga gggaccgcag gcgcttcccg
     3001 acgcggtcgt ccagcggctg acccagctcg ccgagcgtga ggagaaggtg atcctcgctc
     3061 cctactcgca gctcccgcag gggacgagcc tgacgctcgc ggcgtggacg cgggtccagc
     3121 agtgccccgc cgtgcgggac gcgggcgcgg ccgagcaggt ggcacgcggc ttcatccagc
     3181 ggttccgcgg gggcggcgac gcgcccgagc cgacggctcc ctagctcagg agaccgagca
     3241 ggtcccgccg gcggagcatt cgatcgtccc gccgtcgaag cgtcctcgcc agccaccgtc
     3301 ggcgagcctc gtcagggacc gcggcaggcc gaggctcccc gcgtgggcgt cgcgagcgag
     3361 gtaggcgtcg aggaccgggc cgcgcagcca ggtcacggcg gcggttgcgt cgttcaggta
     3421 catccgtcct cgctggaact cctggaagcg tccgccggcg atcgaccgct gggccgacgt
     3481 ggcgcgcccc ggagcgcaca tctgctcgtc gtaggcctcg cggatcttgc cgacgacgtt
     3541 gaggttcttg ccgacgcgga agcgcgtgtc cttcagcccc agcgcgctgc gcaggtccca
     3601 tcccttcacg accttgctgc cggaggtgcc gacgaccttg acggtcttca cgcggccgga
     3661 gatccccagc cggtactcgg tgaagcgggt gatcgtcccg atcgagcccg tcagcgagcg
     3721 caggcgggag gtcagggtgg cgtcggagaa gtcggcggac caggtgcggt tgggattcga
     3781 ggcgaggaag tcgccggggt cacacacggc cttgaggtac ggcgtcgcgg cagcgccgga
     3841 ccagacgttc tcgacgttct cggtgaaccc accgctcgag gaggagtagt aggtcgtgat
     3901 gaggttcccg ttgtggcgta cgacgcgccc gacggtgtcc cgaacggcgg cgacccaacg
     3961 atcgcccatc ggcccgagct ctttctcgcg accgatgaag tactggtcgc gcgtgctcgc
     4021 gacgagcgcg cacgcacagt ccgaccgccg ttgtccgggg gtcgaggcga ccttccggaa
     4081 ggcgtaggtg cgggccgcga tcgcctgcgc ctgaagcgtc gcgccgggcc agctcgacgg
     4141 cacctcggcg atcccgtaca ggtaggcctg gggaccgatg atcgccacgg cgtgcagccg
     4201 gctgctcgag gcgccggcga gctcgaggcg tccgcggttg agcgtgtggc cccactcggc
     4261 caggtggatg gacccaccgt cgagcctcgt gacgcgcagg gcgtcacctg agctccccca
     4321 caggcgcccg gtcgttcccg gcgcgcccac gcggaaccgt ccgtcggtcc gctcgatccg
     4381 ccggcggccg ccagagggga ccgtctcgat caccttcccg ctcgtgagcg tgagccgcat
     4441 ccggccgccg accgcttcga tcgtgacggc cgaccgactc tggacgagcc cgatgcggaa
     4501 gtgcttcgcc ggcggcgaca tctcggcgac cgacgtcccg gagtagtagt gcttgaggat
     4561 gcggttcgcg ctccatccct cctgcgcgag gccgaaggcc ccgtactggc tgaggccgat
     4621 cccgtgcccc cagccggagc cgtggaacgt gaaggtggtg ttggcggagg cgcttccccc
     4681 gagggggaac agcgcgacga cgaccatgat cccgaccaga cgagctctca tcgaacctcc
     4741 acaggggcgt gccccaggag catcgacgcg gtcgaagcgc cgctttagcc cgaagctccg
     4801 gaaagccgcg cttcctccac gaccgtcccg ggctccacgc gcgccccggc gccgaggacg
     4861 cttccgcgca gggtcgcgcc agggccgatg accgcaccgg ccccgacgac cgatccctcg
     4921 acgatcgccc cagatcccac cgtgacaccc tcgtgcagga cggagccgtc gatgcgcgcg
     4981 ccggcggcga tcacgcagcg gggaccgatg acgacgaggt cgcccacgag ggcgtcccga
     5041 tcgacgcgcg ccgtcccggc gaggaggggc cggcgcgcgc atcgacggct ccgtcctgca
     5101 cgagggtgtc acggtgggat ctggggcgat cgtcgaggg
//
LOCUS       DATLGG010000280         5609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_153558, whole genome shotgun sequence.
ACCESSION   DATLGG010000280 DATLGG010000000
VERSION     DATLGG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153558"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VM638_07990"
     CDS             complement(<1..624)
                     /locus_tag="VM638_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HVM12399.1"
                     /translation="MSTAAPPVSRARRRSELGLIFLAVSVAVGAYALAGLGTQGRVPV
                     GLAAYALMLAGGLVAAHLVVRRFAPNADPVLLPAGGLLAGLGFAIIYRLDPERAAAQS
                     GWLLFGLILFAVTLIVIRDHRQLDAFTYTLGFLGVLLLLLPLTPGLGTEVRGARVWIS
                     LGSLRFQPAEVAKVLIVVFAASYLNARKELLAVATRRLGPLALPEPRH"
     gene            complement(621..1763)
                     /locus_tag="VM638_07995"
     CDS             complement(621..1763)
                     /locus_tag="VM638_07995"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HVM12400.1"
                     /translation="MRIRVGAATDVGRVRQRNEDSYLTADPLFAVADGLGGHQGGDVA
                     SRLALEVVSVVASDGGPEDGIPDRLRRKVQEANRTVHQRASEEPDLAGMGTTLTAVVA
                     GRGRLYLAHVGDSRAYLLRDGTLRALTDDHTLVQRLVDEGKLTPEEAAIHPQRSILTR
                     ALGIDEEIEVDQAIVEVKPGDRILLCSDGLTSMVEETEVERIVSEDEDPQAASDRLVA
                     AANEAGGQDNITTVVLDVVESEPAPPTHEASAEAPGVRLDGGFTAPPRRRRRFLVMGG
                     IVVLLLATLLVAGRMYLDRQWFVGVNEGRVALFQGVPVEGLGIELYSVAEETTLPAAE
                     ALRLQPWQGLNEGITAGSREEALQIIAQIERDLRAAEEPEPREEPA"
     gene            complement(1760..2221)
                     /locus_tag="VM638_08000"
     CDS             complement(1760..2221)
                     /locus_tag="VM638_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVM12401.1"
                     /translation="MPPVVLTILKVLFLLLLYFFVWRAVRTIAADLYGRRAARERPRP
                     DQKTRATRRPRSGAPGKVVVLAGRGGKGAAYRLNGTLRLGRDGSCEIRLDDTYASQEH
                     ARISERNGAWVIEDLGSTNGTYLNRNKITVPTQISPGDRIRIGNTHLEVRK"
     gene            complement(2223..2933)
                     /locus_tag="VM638_08005"
     CDS             complement(2223..2933)
                     /locus_tag="VM638_08005"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HVM12402.1"
                     /translation="MSILRDFERRLERIVEGAFTKAFRSGVQPVELAKRALREMEANK
                     TVGVREVWVPNRYVFRLAPEDHERFSGMERGLATELEQVIVEGARERGFGLVASPQVV
                     LERDDSLKRGDLELETELTEATGAPAPTAGGGNPSSATRGPVLALVQDGTTIQEFPLE
                     RDTAVIGRMAGSEIELPDPGASRRHAEVRRRGDGFVLVDLGSTNGTLVNGSEVSEHPL
                     ADGDRITIGHSTLEFRTG"
     gene            3032..3115
                     /locus_tag="VM638_08010"
     tRNA            3032..3115
                     /locus_tag="VM638_08010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3066..3068,aa:Leu,seq:cag)
     gene            3075..3602
                     /locus_tag="VM638_08015"
     CDS             3075..3602
                     /locus_tag="VM638_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HVM12403.1"
                     /translation="MGASPWGFDSPLAHLMTFSETTQSVLILASLGVSGLALFVLASA
                     TSRGRLRQPGGPDREMTKALRAQARAIAELQGAVQQLAGEDRRLAGRLRGAVQRVGLV
                     RYDAFDDMGGRLSFSAAMLDGRGDGFVITSINGRTDNRCYAKPVTGGISEHNLSDEET
                     EAIAIALGKAPVRAS"
     gene            3603..4874
                     /gene="serS"
                     /locus_tag="VM638_08020"
     CDS             3603..4874
                     /gene="serS"
                     /locus_tag="VM638_08020"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVM12404.1"
                     /translation="MLDLRALREDPDPVKVALARRDPALPGDVDRLLDADRRRRELTV
                     QVEGLRAEQNRGSKEVARASGDERQALIASLKDVSARLGKLEPAAAEATEEVQALLAR
                     LPNPPHETAPTGGSDEDSRIEREVGSPPAFGFQARDHVELGTALGFLDIERAAKVSGS
                     RFAYLMGDGVWLQWALVRYCLDVLTARGFLPVIPPVLVREEAMYGTGFLPTDEAQIYA
                     TRDDDLYLVGTSEVPLAGLHQGEILDADALPLRYVGYSTCFRREAGTYGKDTRGIFRV
                     HQFDKVEMFSYTMPGASWEEHEFLLACEEQIIGGLELPYRIANVATGELGASAAKKYD
                     IEVWLPGQGAYRELTSCSNCTDYQARRLGCRVRLPEGNEPVHTLNGTACAVGRMIIAI
                     VENHQREDGSIPLPAALHPYLPEQARVLTPR"
     gene            complement(4768..>5609)
                     /locus_tag="VM638_08025"
     CDS             complement(4768..>5609)
                     /locus_tag="VM638_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12405.1"
                     /translation="TDVSLFAADPLAGRTLGRRLGGFDRLTTPSLAHAELAHRLLALR
                     SLPDVVHNSVEPRSSAAAIRRTWSPPRIAHVSSFRPVKDPALMAEVYLRLRRSTEAEL
                     WLIGDGPGLASTRERIREGGHLEDVRVFGVRTDVEPLLRRTDVCAVTSRTESFSLAAL
                     EAAACGLPVVAPRVGGIPEVVLDGRTGLLLPGGDATAAVRALERLLRDPALRARLGAA
                     AAVRARAFSTARAADAYERIYRSVLGVSVASGPEPAPAGRGAGPPAAGSSRPPAGGSR
                     RSR"
BASE COUNT          797 a         2019 c         2036 g          757 t
ORIGIN      
        1 gtgtcggggc tcggggaggg cgaggggccc gagccgacgc gtcgcgacgg cgagcagctc
       61 cttgcgcgcg ttcaggtagg acgcggcgaa cacgacgatc agcaccttcg ccacctcggc
      121 tggctggaac cgcaagctcc cgagggagat ccacacccgc gcaccgcgga cctcggtgcc
      181 gagccccggc gtgagcggca ggaggaggag cagcaccccg aggaagccga gcgtgtaggt
      241 gaacgcgtcg agctggcggt ggtctcggat cacgatcaat gtcaccgcga agaggatcag
      301 gccgaacagg agccagcccg actgcgccgc cgcgcgctcg ggatcgagcc ggtagatgat
      361 cgcgaacccc agcccggcca ggagcccgcc ggcgggaagg aggacggggt cggcgttcgg
      421 cgcgaaccgg cgcacgacca gatgcgcggc gacgaggcca ccggcgagca tcagggcgta
      481 tgccgccagg cccaccggga cgcgtccctg ggtcccgagc ccggcgagcg cgtaggcccc
      541 gaccgccacc gagaccgcga ggaagatcag gccgagctcc gacctccgcc gcgcgcggga
      601 gaccggggga gcggccgtgc tcacgcgggc tcctcgcgcg gctcgggctc ctccgcggcc
      661 cggaggtccc gttcgatctg agcgatgatc tgcaaggctt cctccctgct ccccgcggtg
      721 atgccttcgt tcagcccctg ccacggctgg agccggaggg cctcggcggc cggaagcgtc
      781 gtctcctcgg ccaccgagta gagctcgatg cccagaccct cgaccgggac gccctggaac
      841 aacgcgaccc ttccctcgtt gaccccgacg aaccactgtc ggtcgaggta catccgtccg
      901 gcgaccagga gcgtcgccag gaggaggacg acgatccctc ccatcacgag gaagcggcga
      961 cgccggcgcg gcggcgcggt gaacccgccg tcgagccgca ccccgggcgc ctcggccgag
     1021 gcctcgtgcg tcggtggggc cggctccgac tccacgacgt cgaggacgac cgtcgtgatg
     1081 ttgtcctggc cgcccgcctc gttggccgct gcgacgagtc ggtccgaagc ggcctgcggg
     1141 tcctcgtctt cggacacgat ccgttcgacc tcggtctcct cgaccatcga ggtcagcccg
     1201 tcgctgcaga gcaggatccg gtcgcccggc ttcacctcga cgatcgcttg atcgacctcg
     1261 atctcctcgt cgatcccgag cgcccgcgtc aggatgctgc gctgcgggtg gatcgccgcc
     1321 tcctcgggag tgagcttccc ctcgtccacg agccgctgga cgagggtgtg gtcgtccgtc
     1381 agcgcgcgaa gcgtgccgtc acggagcagg taggctcgcg agtcgccgac gtgggccagg
     1441 tagaggcgtc ccctcccggc cacgacggcc gtgagggtcg tccccatccc ggccagatcc
     1501 ggttcctcgc tcgcgcgctg gtgaaccgtc cggttcgcct cctggacctt ccggcggagg
     1561 cgatcgggga tgccgtcctc cgggccgccg tcgctcgcga cgaccgagac gacctcgagc
     1621 gcgaggcgcg aggccacgtc gccgccttga tgcccaccga gcccgtcggc gaccgcgaac
     1681 agcgggtcgg cggtgaggta ggagtcctcg ttccgctggc gaacgcggcc gacgtcggtc
     1741 gccgcgccca cgcggatcct cacttgcgga cctccaggtg ggtgttcccg atccggatcc
     1801 ggtcccccgg cgagatctgc gtcggaacgg tgatcttgtt ccggttgagg tacgtcccgt
     1861 tcgtcgagcc gaggtcctcg atcacccagg cgccgttgcg ctcgctgatg cgcgcgtgct
     1921 cctgcgacgc gtaggtgtcg tcgaggcgga tctcgcagga gccgtcacgg ccgaggcgaa
     1981 gcgtcccgtt cagccggtag gcggcgccct tgccgccacg tcccgcgagg accacgacct
     2041 tgcccggggc gccgctccgg gggcggcggg tcgctcgcgt cttctggtcc gggcgcggcc
     2101 gctcgcgcgc cgcgcgtctt ccgtagagat cggcggcgat cgtgcggacc gcgcgccaga
     2161 cgaagaagta gagcaggagc aggaagagca ccttcaggat cgtcaggacg acgggcggca
     2221 tctcagcccg tccggaactc gagggtcgag tgaccgatcg tgatccggtc cccgtcggcg
     2281 agcggatgct ccgagacctc ggagccgttc acgagcgtcc cgttcgtgga cccgaggtcg
     2341 acgaggacga agccgtcgcc gcggcggcgg acctccgcgt gccgccggct cgccccgggg
     2401 tccggcagct cgatctccga cccggccatg cgcccgatca ccgcggtgtc acgctccagc
     2461 gggaactcct ggatcgtcgt cccgtcctgg acgagcgcga ggaccggacc gcgcgtggcc
     2521 gagctgggat tcccaccgcc ggcggtgggg gcgggggcgc cggtcgcctc ggtcagctcc
     2581 gtctcgagct cgaggtcgcc gcgcttgagg gagtcgtccc gctcgaggac cacttggggg
     2641 gaggcgacga gaccgaagcc acgctcccgc gcgccctcga cgatcacctg ctcgagctcg
     2701 gtcgcgaggc cacgctccat cccggagaac cgctcgtggt cctccggggc gaggcggaag
     2761 acatagcggt tcggtaccca gacctcccgg acgccaaccg tcttgttcgc ctccatctcg
     2821 cggagcgcac gcttcgcgag ctccacgggc tggacccccg accggaacgc cttcgtgaac
     2881 gcgccctcga cgatccgctc gaggcgccgt tcgaagtcgc ggaggatgct catcgggtcc
     2941 ttcctgtggg gtgggagacg attataggtg ggcatcccgg gcggtccgga aacgccttcc
     3001 gaggcacctg atacagtgac gccgttgccc cgggcgggtg gcggaattgg tagacgcgca
     3061 ggattcagga tcctgtgggc gcaagcccat gggggttcga ctcccccctc gcccacctga
     3121 tgacgttctc cgagacgacg cagagcgtcc tgatcctcgc gagcctcggc gtctccgggc
     3181 tcgccctgtt cgtcctcgcc tccgcgacgt cgcgcggacg gctgcgccag cccggcgggc
     3241 ccgatcgcga gatgacgaag gcgctgcgcg cgcaggcgcg cgcgatcgcc gagctgcagg
     3301 gcgccgtcca acagctcgcg ggcgaagacc gccggctggc cgggcggctc cgcggagcgg
     3361 tccagcgggt cgggctcgtc cgctacgacg ccttcgacga catggggggg cgtctgtcct
     3421 tctccgcggc gatgctcgat gggcgcggcg acggcttcgt gatcacctcg atcaacggcc
     3481 ggaccgacaa ccgctgctac gcgaagccgg tgaccggcgg catctcggag cacaaccttt
     3541 cggacgagga gaccgaggcg atcgccatcg cgctcggcaa ggcccccgtc cgcgcctcgt
     3601 aggtgctcga cctccgcgca ctccgcgagg acccggatcc cgtcaaggtc gcgctcgccc
     3661 gacgcgaccc cgcgcttccc ggcgacgtcg accgcctcct cgacgcggat cgccgccggc
     3721 gagagctgac cgtgcaggtc gaagggctgc gcgccgagca gaaccgcggc tcgaaggagg
     3781 tcgcccgtgc atcgggagac gagcggcagg cgctgatcgc ctcgctgaag gatgtgtcgg
     3841 ctcgcctcgg gaagctcgag cccgcggcgg ccgaggcgac cgaagaggtg caggcgctcc
     3901 tcgcgcggct cccgaacccc ccgcacgaga cggccccgac cggcgggtcg gacgaggaca
     3961 gccggatcga gcgcgaggtc ggctctccgc ccgcgttcgg gttccaggct cgcgatcacg
     4021 tcgagctcgg cacggcgctc ggcttcctcg acatcgaacg ggccgcgaag gtctccggct
     4081 cccgcttcgc gtacctgatg ggggacggcg tctggctcca gtgggcgctc gtccgctact
     4141 gcctcgacgt gctcaccgcg cgcgggttcc tgccggtgat cccgccggtg ctcgtccgcg
     4201 aggaggcgat gtatgggacc gggttcctcc cgacggacga ggcgcagatc tacgcgacgc
     4261 gcgacgacga cctctacctg gtcggcacat ccgaggtccc gctcgcgggg ctccaccagg
     4321 gggagatcct cgacgccgac gcgctgccgc tgcgctacgt gggctactcg acgtgcttcc
     4381 gccgcgaggc gggaacgtac gggaaggaca cgcgcggcat cttccgggtg caccagttcg
     4441 acaaggtcga gatgttctcc tacacgatgc cgggcgcctc gtgggaggag cacgagttcc
     4501 tgctcgcgtg cgaggagcag atcatcggcg gcctcgagct cccctaccgc atcgcgaacg
     4561 tcgcgacggg tgagctcggc gcgtcggcgg cgaagaagta cgacatcgag gtctggctgc
     4621 cgggccaggg cgcgtaccgc gagctcacgt cgtgctcgaa ctgcacggac taccaggcgc
     4681 ggcgcctcgg gtgccgcgtg cggctgcccg aggggaacga gccggtgcac accctgaacg
     4741 ggacggcgtg cgcggtcggg cgcatgatca tcgcgatcgt cgagaaccac cagcgggagg
     4801 acggctcgat cccgctgccg gcggccctgc acccctacct gccggagcag gctcgggtcc
     4861 tgacgccacg ctgacgccga gcaccgaccg atagatgcgc tcgtaggcgt cggccgcgcg
     4921 cgccgtcgag aaggcgcgcg cccgcaccgc tgccgccgcg cccaggcgcg cacgcagcgc
     4981 ggggtcgcgg aggaggcgct cgagggctcg gaccgccgcc gtcgcgtcgc caccggggag
     5041 gaggagcccg gtacggccgt cgagcacgac ctcggggatg ccgccgaccc ggggcgcgac
     5101 gaccggaagc ccgcacgccg ccgcttcgag cgcggcgagc gagaagctct ccgtgcggct
     5161 cgttaccgcg cagacgtccg tccgtcgcag gagcggctcg acgtcggtcc gcacaccgaa
     5221 gacccgcacg tcctcgaggt gcccgccctc gcggatccgt tcgcgcgtcg acgcgagccc
     5281 gggaccatcg ccgatcagcc acagctccgc ttcggtcgac cgccggaggc ggaggtagac
     5341 ctcggccatg agcgccggat ccttcaccgg acggaagctg gagacgtgcg cgatccgcgg
     5401 cggggaccag gtcctccgga tcgcggccgc ggacgaccgg ggttcgacgg agttgtgcac
     5461 gacgtcgggc agggaccgaa gcgccaggag gcgatgggcg agctcggcgt gggcgagcga
     5521 cggggtcgtg agccggtcga agccgccgag ccgccggccg agggtcctgc ccgcgagcgg
     5581 atcggcggcg aacaggctca cgtcggtgc
//
LOCUS       DATLGG010000281         4770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_184145, whole genome shotgun sequence.
ACCESSION   DATLGG010000281 DATLGG010000000
VERSION     DATLGG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184145"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VM638_08030"
     CDS             <1..1424
                     /locus_tag="VM638_08030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HVM12406.1"
                     /translation="LGQGSVIGRRLELPAVRADGTELQVELTVTRIELPGPAQFTGIL
                     RDLTAEHAARRERERLLEREREARREAERARAGLEFLVDAGATLAASLEEGEAMDRIA
                     RLLVPRLAEWCAIDLVAPDGTIRRAAVAHIEVEEWATIGAGRSGSLVEEAIRDGEPRL
                     IPALEPLHGERLGTDVDVLELRCAGIFPLLAHDTTLGALVVARRNRYDDDEIALLREL
                     ASRASVALQNARLYEDRTYVARTLQRSLLPTVLPEVPGLEVAVRYRAAGEGNEVGGDF
                     YDVFALADGTWCAAIGDVGGKGPDAAALTGLARHTLRTAGMREHRPAELLRTLNEAIV
                     REGTERLCTVSLTRIRPTEGAYEITIACGGHPSPLLLRSDGEVVRPSVSGTLLGAIAE
                     PQIGEVTMSLASGDSLVSFTDGLIDESGAGRLSIEEAVAILRTHAGEAAEGLVDAVEG
                     AALRAKSEPPRDDIALLCLRVLP"
     gene            complement(1425..2096)
                     /locus_tag="VM638_08035"
     CDS             complement(1425..2096)
                     /locus_tag="VM638_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HVM12407.1"
                     /translation="MVTSPIFDVREIRISGAARLDRARVLEVAGVREGMNLLGLDLRG
                     VERSLAREPWVAAATVRRDLPSTVHLDLTERIAAGVITDPRGTVIVASDGVVLERADG
                     VPAARPSLGRSDRILRPGSRAPTPRPLLRVVASLPEALRRHVARVRTGRDGVELDLAR
                     GGVVLYGPADQLQAKNAALRQILRYARREGIAIGYIDVRSPTGPALKPAGSPDPAEPV
                     PTLEP"
     gene            complement(2167..3099)
                     /gene="murB"
                     /locus_tag="VM638_08040"
     CDS             complement(2167..3099)
                     /gene="murB"
                     /locus_tag="VM638_08040"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HVM12408.1"
                     /translation="MADREAIDRVAADLARHAGRRLRRAFPLAPLTSFRLGGPAALYL
                     EVLSIEDLEAAGAALEGTEVPVLVIGKGSNLLVSDDGFPGLVLRLGRPFRWSARDGNR
                     LTAGGAMPLPALAGVALTHHLAGLEFGVAIPASLGGSVRMNAGAHGHALDEVLERIEV
                     FVLPERSTWTLAAADAGFGYRTSALPAGGVVTAATAALRPGDPAEIRARMDEARDWRR
                     RTQPLAEPNCGSVFKNPPGHHAARLIEEADGKALSVGGARVSEKHANFVIAGPGARAA
                     DVDRLIRLVQERVLERSGVALETEVHRVGRFDAP"
     gene            complement(3092..4513)
                     /gene="murC"
                     /locus_tag="VM638_08045"
     CDS             complement(3092..4513)
                     /gene="murC"
                     /locus_tag="VM638_08045"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HVM12409.1"
                     /translation="MRPYVPPDGSIPTLPVPDLSGLRRVHLMGIAGAGMSGIAHLLLD
                     RGFAVSGCDLKGSTGLNALRARGAEIAVGHDPAHHAGVDAVVISSAIRPGDAELAAAR
                     EAGIPVLHRAQVLAALAGTERSVAVAGTHGKTTTTSMLAVALVAAGLDPTYVIGGELN
                     ESGSNARSGTGGVFVAEADESDGSFLLMRPSIGVVTNVEADHLDFFGTLEEVEAAFAA
                     FCARCDHLVACGDDPGTRRVLALAGREATTYGEKDDNDVVLHIGELGPEASGRVRIGA
                     EEIDLRLRVPGRHCLLNATAAVAVVAALGGSAAEAAAALAGFRGVRRRFEHRSSIGGA
                     RVVDDYAHHPSEIEATLAAAATERPERMVAVFQPHRYSRTAALGRELGRSLAAADVVV
                     VTDVYAAGEHPVPGVSGKMVVDGLVEAAPGKRAIYLPSRADVAPFVARELRRGDLILT
                     LGAGDVTMVGEEVVRHVGGEDRG"
     gene            complement(4516..>4770)
                     /locus_tag="VM638_08050"
     CDS             complement(4516..>4770)
                     /locus_tag="VM638_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HVM12410.1"
                     /translation="GREISIADLVELLFEVTGRRKDLVSDEARIRPKDSEVHRLLCDA
                     TRAREWAGWTPQVSLEEGLRRTAGWVRDHLDDLTATRYHV"
BASE COUNT          675 a         1726 c         1764 g          605 t
ORIGIN      
        1 agctcggcca ggggagcgtg atcggacgac ggctggagct tcccgcggtg cgcgcggacg
       61 gaacggagct ccaggtcgag ctgacggtga cacgcatcga gcttcccggg cctgcgcagt
      121 tcaccggcat cctgcgcgac ctcaccgccg agcacgctgc gcgacgggag cgcgaacgcc
      181 tcctcgagcg agagcgtgag gctcggcgtg aggcggaacg ggctcgtgcc ggcctcgagt
      241 tcctcgtcga cgccggtgcg acactcgcgg cgtcgctcga agagggcgaa gccatggatc
      301 ggatcgcgcg cctgctcgtc cctcgcctgg cggagtggtg cgcgatcgac ctggtcgcgc
      361 cggacggcac gatccgacgc gcggcggtcg cgcacatcga ggtcgaggag tgggccacga
      421 tcggggcggg ccggagcgga agcctggtgg aggaggcgat ccgcgatgga gagccgcgtc
      481 tgatcccggc gttggagccg ctccatgggg agcggctggg gacggatgtc gacgtgctcg
      541 agttgaggtg cgcggggatc ttcccgctgc tggcgcacga cacgacgctc ggggcgctcg
      601 tcgtggcgcg tcgcaaccgc tacgacgacg atgagatcgc gctcctgcgc gagctggcct
      661 cgcgggcgtc ggtcgcgctc cagaacgcgc ggctgtacga ggaccgcacc tacgtcgcgc
      721 gaaccctgca gcggagcctg ctgccgacgg tgcttcccga ggtcccagga ctcgaggtcg
      781 ccgtccgcta ccgcgccgcc ggcgagggga acgaggtcgg cggggacttc tacgatgtct
      841 tcgccctcgc ggacggcacg tggtgcgcgg cgatcggcga cgtcggtggc aagggccccg
      901 acgcggcggc actcacgggg ctcgcgcgcc acaccctgcg gaccgctggg atgcgcgaac
      961 accgcccggc cgagctcctg cgcaccctga acgaagcgat cgtgcgggag gggacggagc
     1021 gcctctgcac ggtgtccctc acccggatcc gcccgacgga gggggcctac gagatcacga
     1081 tcgcatgcgg aggtcatccg tcgccgctcc tgctccgctc cgacggggag gtcgtgcggc
     1141 cgtcggtgag cggaaccctg ctcggcgcga tcgcggagcc gcagatcggg gaggtcacga
     1201 tgagtctcgc gtccggcgac agcctcgtct cgttcaccga tgggctgatc gacgaaagcg
     1261 gcgccgggcg cctgtcgatc gaagaggccg tggcgatcct gcggacccat gccggggagg
     1321 ccgcagaggg gttggtggat gcggtcgagg gggcggcgct ccgcgcgaag tcggagccgc
     1381 cgcgcgacga catcgccctg ctctgcctgc gggtcctccc ctagctacgg ctcgagcgtc
     1441 ggaacgggct ccgccgggtc aggtgaaccc gccggcttga gggccgggcc cgtcggcgac
     1501 cggacgtcga tgtaaccgat cgcgatgccc tcgcgccgtg cgtaccgcag gatctggcgg
     1561 agcgcggcgt tcttcgcctg gagctggtcg gccgggccgt agaggaccac cccgccacgg
     1621 gcgaggtcga gctcgacgcc gtcacggccg gtgcggacgc gggcaacgtg ccggcggagc
     1681 gcctccggga gggacgccac cacccgcagc agcggccgcg gtgtcggtgc ccgactcccc
     1741 ggacgcagga tgcggtccga gcgaccgagg gaggggcggg ccgcgggcac cccgtccgcc
     1801 cgttcgagga cgaccccgtc ggaggccacg atgaccgtcc cgcgcgggtc ggtgatcaca
     1861 ccggcggcga tccgctcggt gaggtcgagg tggacggtcg aggggaggtc ccgtcgcacg
     1921 gtcgctgccg cgacccacgg ctcacgcgcg aggctccgct cgacgccgcg caggtccagc
     1981 cccaacaggt tcatcccctc gcggacgccg gccacctcga gcacgcgcgc gcggtcgaga
     2041 cgggcggctc cgctgatccg gatctcgcgc acgtcgaaga tcggcgatgt caccacggcc
     2101 caagccgccg cggctacggc gacgacagcg acgaccgcgc tcgtccggcg ggaggggcga
     2161 cgtcggctac ggcgcatcga accgaccgac gcggtgcacc tccgtctcga gggcgacgcc
     2221 ggatcgctcg agcacacgct cctgcacgag acggatcaga cggtcgacat ccgccgcgcg
     2281 cgcccccggt ccggcgatca cgaagttcgc gtgcttctcc gaaacgcgcg ccccaccgac
     2341 ggagagcgcc ttcccgtccg cctcctcgat cagccgcgcg gcatgatggc ccggcggatt
     2401 cttgaacacg ctgccgcagt tcggctcggc gagcggctgg gttcgtcgcc gccagtcgcg
     2461 cgcttcgtcc atgcgcgcac ggatctcggc gggatccccg ggccgcaggg cggccgtcgc
     2521 ggccgtcacg acaccgccgg cgggaagggc ggaggtccga tatccgaacc cggcgtcggc
     2581 cgctgcgagc gtccacgtgg accgctccgg gaggacgaag acctcgatcc gctcgagcac
     2641 ctcgtcgagc gcgtgaccgt gggcgccggc gttcatccgc accgatcccc cgagcgaggc
     2701 cgggatcgcg acgccgaact cgagccctgc cagatggtgc gtgagcgcga cgccggcgag
     2761 ggccggcagg ggcatcgcgc cgccggccgt caggcggttc ccgtcgcggg cggaccagcg
     2821 gaacggccgc ccgaggcgga ggacgaggcc ggggaagcca tcgtcggaga ccaggaggtt
     2881 cgagcccttc ccgatcacga gcacggggac ctcggtcccc tcgagcgcag cgccggcggc
     2941 ctcgagatcc tcgatcgaga ggacctcgag gtagagcgcg gcagggccgc cgagccggaa
     3001 ggaggtcagc ggggcgaggg ggaaggcgcg gcggaggcga cgtccggcgt gtctcgccag
     3061 atcggccgcg acgcggtcga tcgcctcacg gtcagccacg atcctccccg ccgacgtggc
     3121 ggacgacctc ctcgccgacc atcgtgacgt ctccggcacc gagggtgagg atcaggtccc
     3181 cgcgccggag ctcccgggcg acgaacggcg cgacgtccgc ccggctcggc aggtagatcg
     3241 cgcgcttccc gggagccgcc tcgacgaggc cgtcgacgac catcttcccg ctgacgccgg
     3301 gcacgggatg ctccccagcc gcgtacacgt cggtcaccac gacgacgtcc gccgcggcga
     3361 ggctgcgacc cagctcccgc ccgagcgccg ccgtccgcga gtagcgatgg ggctggaaca
     3421 cggcgaccat ccgttccggc ctctccgtcg cggccgcggc gagggtggcc tcgatctcgc
     3481 tcggatgatg cgcgtagtcg tccacgaccc gcgcgccgcc gatcgagctt cgatgctcga
     3541 agcgacgtcg caccccgcgg aacccggcga gcgcggccgc cgcctcggcc gcggagcccc
     3601 cgagcgccgc gacgaccgcg acggccgcgg tggcgttcag caggcagtgc cgacccggga
     3661 cgcggaggcg gagatcgatc tcctccgctc cgatccgcac gcgcccgctg gcctccggtc
     3721 ccagctctcc gatgtgcagg acgacgtcgt tatcgtcctt ctcgccgtac gtcgtcgctt
     3781 cccgtccagc gagcgcgagg acgcgccgcg tcccggggtc gtcgccacac gcgacgaggt
     3841 ggtcgcaccg tgcgcagaag gcggcgaagg ccgcctcgac ctcctcgagc gtgccgaaga
     3901 agtcgaggtg gtcggcctca acgttcgtga cgacgccgat cgatggacgc atgagcagga
     3961 aggagccgtc ggactcatcg gcctcggcca cgaagacacc gccggtcccc gagcgcgcgt
     4021 tgctcccgct ctcgttcagc tcgccgccga tcacgtacgt cggatccagc cccgccgcca
     4081 cgagcgcgac cgcgagcatc gaggtggtcg tcgtcttgcc gtgcgtgccc gccaccgcga
     4141 cgctgcgctc cgtccccgcg agcgcggcca ggacctgtgc gcgatgcagg acggggatgc
     4201 ccgcctcccg cgcggcggcc aactccgcat ctcccggacg gatcgcgctc gagatgacga
     4261 ccgcgtcgac gcccgcgtgg tgcgccgggt cgtgcccgac ggcgatctcc gcccctcggg
     4321 cgcgcagcgc gttcagtccc gtcgatccct tgaggtcgca gccggagacg gcgaaaccgc
     4381 ggtcgagcag cagatgggcg atcccgctca tgccggcgcc ggcgatgccc atgaggtgga
     4441 cgcggcggag ccccgagaga tccggaacgg gaagcgtggg gatgctgccg tcagggggga
     4501 cgtagggacg catgctcaga cgtggtaacg cgtcgccgtg agatcgtcga ggtgatcgcg
     4561 gacccatccg gcggtgcgcc ggagaccctc ctcgagcgag acctgcgggg tccacccggc
     4621 ccactccctc gcgcgcgtcg catcgcacag gagccggtgg acctcgctgt ccttcggacg
     4681 gatccgtgcc tcgtcgctca cgagatcctt gcgtcgaccg gtgacctcga acagcagctc
     4741 cacgagatcg gcgatcgaga tctcgcggcc
//
LOCUS       DATLGG010000282         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_184229, whole genome shotgun sequence.
ACCESSION   DATLGG010000282 DATLGG010000000
VERSION     DATLGG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184229"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VM638_08055"
     CDS             complement(<1..1379)
                     /locus_tag="VM638_08055"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HVM12411.1"
                     /translation="MKAWVLALGFTAIGMSAAATARAEQQGDGTDTGRVSHIEGTLFG
                     RGPYDEEVSELAVNAIIRAGDEIFTDEGTFAELELPGETFLRLAGATTVVVQRLDVNG
                     AEIALADGAAYLSRGPDAAPARFKSFAGTVDLGGRAMVRIGNDPQRTRLEIGVADGNA
                     ELRDDRTAITVRAGQRLVWTKGSTRDEVFRVKDGDAFDRWNDERERRVRGANRPQHVA
                     RGVMGSYELDGHGTWVLIDGYWAWRPTVVVSWRPYRHGYWSWCEPYGWTWVSYEPWGY
                     TTSHYGRWYYRAHYGWVWYPDAYWGPAWVVWAGFGPYLGWAPCDFWGRPIYVHSYYSY
                     YDYGAWCYTDAHYFYYGGGYYYRHDYRRRRTTTTTTREPYATSSGRSAGAGRGGGGGI
                     GRDGKYQIREFDRETIAKYTPVPVKDARADLTPKHIVAAADRGALKGPARGARSGDFL
                     ERQVRDPEG"
     gene            complement(1526..2215)
                     /locus_tag="VM638_08060"
     CDS             complement(1526..2215)
                     /locus_tag="VM638_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HVM12412.1"
                     /translation="MPDDRPESVRSPNATPDAPEMPAEDRKAQQPEVGRPDRGPEVGT
                     PDRGPEAPEPPSEPPRESADGGTESRAESPRDPAKDPAEEDPRIGELQRLVVHLKADF
                     DNYRKRSQKEIASSMRMGELEAVKRILPVVSNLERALAAAGQMEGPGQALAEGVRQIH
                     QQLVRVLASMQIERIPGVGTPFDPMLHDAVAATSNPDVPPGTVVDEFEPGWQADGKAL
                     VPAKVRVSTAE"
     gene            2258..>2743
                     /locus_tag="VM638_08065"
     CDS             2258..>2743
                     /locus_tag="VM638_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HVM12413.1"
                     /translation="MGRHVTSGKVSVIAADSRFLRSLSRRAWLFGTSGALAALRASPA
                     DARVDPRIDALAASLSLEKKLGQLLMIGVSGTEMTPETRSLLGELDPGAVILLGRNVR
                     EPKQLAALNASLQDGAAIPRFIAMDQEGGIVVRLSRGATVFPGAMATGATGSSNLAYL
                     EG"
BASE COUNT          353 a         1015 c          962 g          413 t
ORIGIN      
        1 atcccctcgg gatcccgcac ctggcgttcg aggaagtcgc cgctccgcgc cccgcgcgcg
       61 gggcccttca gcgcgccccg atcggccgcc gccacgatgt gcttcggcgt gaggtccgcg
      121 cgcgcgtcct tcaccgggac cggcgtgtat ttcgcgatcg tctcgcggtc gaactcgcgg
      181 atctggtact tcccgtcgcg accgatgccg cctccgcccc cgcgcccggc tcccgcgctc
      241 cgtcccgagg aggtggcgta gggctcccgc gtggtggtcg tcgtcgtgcg gcgccggcgg
      301 tagtcgtgcc ggtagtagta gccgccgccg tagtagaagt agtgcgcgtc cgtgtagcac
      361 cacgcgccgt agtcgtaata gctgtagtag ctgtggacgt agatggggcg gccccagaag
      421 tcgcacggcg cccacccgag gtacggtccg aagccggccc agacgaccca cgccggaccc
      481 cagtaggcgt cgggatacca gacccacccg tagtgcgcgc ggtagtacca acggccgtag
      541 tgcgacgtcg tgtagcccca cggctcgtag gagacccacg tccagccgta cggctcgcac
      601 cacgaccagt agccgtggcg gtacgggcgc cagctcacga cgacggtcgg acgccacgcc
      661 cagtagccgt cgatgagcac ccacgtgccg tggccgtcga gctcgtacga ccccatgacg
      721 ccgcgggcga cgtgctgcgg ccggttcgcg ccgcgcacgc ggcgctcgcg ctcgtcgttc
      781 cagcggtcga acgcgtcgcc gtccttcacg cggaacacct cgtcgcgggt cgagcccttc
      841 gtccagacga gtcgctgccc cgcgcgcacg gtgatcgccg tgcggtcgtc gcggagctcc
      901 gcgttgccgt cggcgacgcc gatctccagg cgcgtgcgct gcgggtcgtt gccgatgcgg
      961 accatcgcgc ggccgccgag gtcgaccgtc cccgcgaacg acttaaacct cgccggggcc
     1021 gcgtcagggc cgcgcgagag gtacgccgcg ccgtcggcga gcgcgatctc ggcgccgttc
     1081 acgtcgaggc gctggaccac gacggtcgtc gcgcccgcca ggcggaggaa cgtctcgccg
     1141 gggagctcca gctcggcgaa ggtgccttcg tcggtgaaga tctcgtctcc cgcgcggatg
     1201 atcgcgttga ccgcgagctc cgagacctcc tcgtcgtagg ggccgcggcc gaagagcgtc
     1261 ccctcgatgt gggacacccg gccggtgtcg gtgccgtcgc cctgctgctc tgcgcgcgcg
     1321 gtcgcggccg ccgacatccc gatggcggtg aaccccagcg caagaaccca agccttcata
     1381 aagcctccaa agccaaccta gcacgcggtc ctaagtaccc gaattcgaat gggccttcgg
     1441 ctcgcgtcgg aaccacccgt agcgacggac cgaggggggc ccgaattcga gaatctgagg
     1501 tgaaaaatct ccgtccgccg cggcgttatt ccgcggtcga gacgcggacc ttggccggga
     1561 cgagcgcctt cccgtccgcc tgccagccgg gctcgaactc gtcgacgacc gtccccgggg
     1621 ggacgtccgg gttcgacgtc gccgcgacgg cgtcgtgcag catggggtcg aacggcgtgc
     1681 cgacccccgg gatgcgctcg atctgcatcg aggccaacac gcgcacgagc tgctggtgga
     1741 tctggcggac gccctcggcg agcgcctgcc ccggcccctc catctgcccg gcggccgcga
     1801 gcgcgcgctc gagattcgac acgacgggga ggatccgctt caccgcctcg agctccccca
     1861 tccgcatcga cgaggcgatc tccttctgcg agcgcttccg gtagttgtcg aagtccgcct
     1921 tcaggtgcac gacgagccgc tgcagctcgc cgatgcgcgg gtcctcctcg gccggatcct
     1981 tggccggatc gcgcggggac tccgcgcggc tctccgtccc gccgtcggcc gactcgcgcg
     2041 gcggctcgct cgggggctcc ggcgcctccg gtccgcgatc gggcgtgccg acctccggtc
     2101 cgcggtcggg acggccgacc tccggctgct gcgccttccg gtcctcggcc ggcatctcgg
     2161 gggcatcggg cgtggcgttc ggcgagcgga cggactcggg gcggtcgtcg ggcatggggc
     2221 ctcgatctaa gcagccgggc gccccgtgtc aacgcggttg gggaggcacg taacgagtgg
     2281 taaagtctcg gttatcgccg cggactcccg cttcctccgc tcgctgtccc gccgcgcgtg
     2341 gctcttcggg acctccggcg cgctcgccgc gctccgcgcg tcgccggccg acgcgcgcgt
     2401 cgacccgcgc atcgacgcgc tcgcggcctc gctctcgctc gagaagaagc tcggccagct
     2461 cctgatgatc ggcgtctcgg ggacggagat gacgcccgag acgcgctcgc tcctcggcga
     2521 gctcgacccg ggcgcggtga tcctgctcgg gagaaacgtc cgggagccga agcagctcgc
     2581 ggccctcaat gcgtcgctcc aggacggcgc ggcgatcccg cggttcatcg ccatggacca
     2641 ggagggcggg atcgtcgtgc ggctctcgcg cggggcgacg gtctttcccg gcgcgatggc
     2701 gacgggtgcg accggctcct cgaacctcgc ctacctcgag ggg
//
LOCUS       DATLGG010000283         6537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_200707, whole genome shotgun sequence.
ACCESSION   DATLGG010000283 DATLGG010000000
VERSION     DATLGG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200707"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1335
                     /locus_tag="VM638_08070"
     CDS             70..1335
                     /locus_tag="VM638_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12414.1"
                     /translation="MDAERAKDLQRSQLSMVAVRWTGAVFAAYQVLVFDNRPYPSPTF
                     RTLGLALAATLAVGNLAIFLTTRRVSTLRGARAIAICGILLDVSVASGFVYLFAFDPL
                     SALWAVLLILPMEGAIMFQLRGALFTWAATLALYVPREAWGAQRFPAEYPLGVQWSSI
                     TFRMGIALLIGMVAGLMARDLVRQRTQLRQTLGRVQRLDRMRQELVNTLAHDVRNPLT
                     AIRGTLTLLLTRSDKVDAETGRELLGNADRQARRLETLAEDLLELARLEKGRLDLAIQ
                     YVTLRETVERGLSFTDHESAYRIDIPEDLRLRADPGRLEQIVVNLASNALRYGEAPFE
                     ARAHVDGDAVVLTLRDHGPGIPESEARHLFDPFRAEQKKGSVGLGLAIVKGLAEAHGG
                     TVRYLPNEPRGACFEVRLPLTGPREPATV"
     gene            1382..2914
                     /gene="hutH"
                     /locus_tag="VM638_08075"
     CDS             1382..2914
                     /gene="hutH"
                     /locus_tag="VM638_08075"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVM12415.1"
                     /translation="MEPLLLTGTALPLEAIEAVARRGAPVRLADEAAAAVRSSRDLVE
                     QHLGRDRPIYGINTGIGALADTTIPAGDLEQLQRNIVRSHSAGVGTLLPAEVVRAVIL
                     LRARTMATGRTGVRVEVIERLLELLNRGITPAVPSRGSVGASGDLAQLAHIGQCVIGE
                     GAVLHGDGVRPAAEVLREEGLEPLELATKEGLALINGTEGMVALGALALVDLGRLLRA
                     ADVAAAMTVEAALGTDRPFAEDLMELRPHPGQAASAANLRAILRDSPVIASHRDSTHA
                     VQDPYSMRCAPQVHGAARETHAFASMVLGRELGAVTDNPVVLPDGRVESAGNFHGEPL
                     AYALDLLAIGATGLASISERRTFWLLGPGTSRDLPPFLSTQPGLGSGYMLAQYTQAAL
                     VSEAKGLSHPAGLDSIPTSGSQEDHVSMGWTSGLKLREVIGHLRSVIAIEVLCAAQAL
                     DLRRPIAPAAGTAAALAAVRERVPFLDQDRDLSVDIEAVTGLVAGGELVQRVEDAVGP
                     LA"
     gene            2928..4589
                     /gene="hutU"
                     /locus_tag="VM638_08080"
     CDS             2928..4589
                     /gene="hutU"
                     /locus_tag="VM638_08080"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVM12416.1"
                     /translation="MAIVRTIRAPRGPERSCKGWPQEAALRMLMNNLDPDVAERPQDL
                     IVYGGTGRAARSWEAFDAIVRTLRDLDDDETLLVQSGKPVGVFRTHEHAPRVLISNSM
                     LVPRWATHETFRDLEDQGLTMYGQMTAGSWIYIGTQGILQGTYETLAELASQRFDRTL
                     RGRIVLTAGLGGMGGAQPLAVTMNEGVALCVEVDPARIERRIRTRYLDRATEDLDEAI
                     AWAIEARDAGRALSIGLRGNAAEVVPELLRRDFPADAVTDQTSAHDPLDGYVPHGLPL
                     DRADELRRSDPDTYRKRAHEAMAVHCEAMVGFLDRGSVVFDYGNNLRAGAREGGFERA
                     FAYPGFVPAFVRPMFCEGKGPFRWAALSGDPEDIATTDRAIAELFPQDERLHRWLGMA
                     RERVAFQGLPARICWLGYGERHLAGLRFNELVASGAVSAPIVIGRDHLDSGSVASPYR
                     ETEGMRDGSDAIADWPILNALVNTAAGAHWVSVHHGGGVGIGYAIHAGMVVVADGSDD
                     AARRLERVLTTDPGTGVMRHADAGYDEALRVARDRGVRLPMLEGE"
     gene            4586..5497
                     /locus_tag="VM638_08085"
     CDS             4586..5497
                     /locus_tag="VM638_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HVM12417.1"
                     /translation="MTDDPNWPRSSAWLSGDRDRARTAAIAVLGVPIRRGSISGGRFD
                     LAPGAVRSALGRFSTWDGERDLGAVRAVDLGDLDVASQTVEEARHTIERSIGEAPGRA
                     PLVLLGGDNSITWAAASALSAEGGPVGLITLDAHHDLRGTDHGISNGAPVRALLEDGT
                     IAGGNVWQIGIQPFANSPAYAAVATDHGIHVTRPEEVRARGIGSVMDDALADLDARCD
                     VIYVDLDVDVLDRAFAPACPGARPGGLLPWEVHEAARLAGAHPKVRAMDLVEIDPERD
                     VADVTVLNAARCLLGFAAGVATRKSAD"
     gene            5557..6474
                     /locus_tag="VM638_08090"
     CDS             5557..6474
                     /locus_tag="VM638_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12418.1"
                     /translation="MPRRLRTLGVIAIVALGLTATPAAGDHTDPRDPLSPTSPGLPGN
                     GIVQGAGRWEHIRNFPPNPGSDLEFFRKGGDIYASTGTLGQGEAGHVGQRIIRLTHNG
                     AVNPTWVADHGSAACRTANPTGTLGLQHDVQITPKHNPQLVIDATDATGRCHDPAGGG
                     LEFVDISGIGPKSRFQPREIHLTRHAGLSHTVTVDTVRPWIVYNSTSDSSGANRPWID
                     VLDIRTCLGRTGDTIAQKRARCRPLVYRIPFEPEWSQRVRPNGELEPGSESSCHDITS
                     VGSRIYCAGLNATLIFDTSKMRVAFRPAQ"
BASE COUNT          928 a         2457 c         2269 g          883 t
ORIGIN      
        1 ccgggcgccc ctctcatctc gcgaacgatg ccgcggcgcg tgcgatccgc ctggtagcat
       61 cggcccagcg tggacgccga acgggcgaaa gacctgcaac gttcgcagct ctcgatggtc
      121 gccgtccgct ggaccggcgc cgtcttcgcc gcctaccagg tcctggtctt cgacaaccgg
      181 ccgtacccct cgcccacctt ccgcacgctc ggcctggcgc tggcggccac ccttgccgtc
      241 gggaacctcg cgatcttcct gacgacccgg cgcgtctcga ccctgcgcgg cgcccgggcc
      301 atcgcgatct gcgggatcct gctcgacgtc tcggtcgcgt cggggttcgt ctacctgttc
      361 gcgttcgacc ccctgtccgc cctctgggcc gtcctgctga tcctcccgat ggagggagcg
      421 atcatgttcc agctccgcgg cgcgctcttc acctgggcag cgacgctcgc tctgtacgtc
      481 ccccgggagg cctggggcgc ccaacgcttc cccgccgagt accccctcgg ggtgcagtgg
      541 agcagcatca cgttccggat ggggatcgcc ctcctcatcg gcatggtggc ggggctcatg
      601 gcccgagatc tcgtccggca gcggacgcag ctccgacaga ccctcggccg ggtccaacgg
      661 ctcgaccgga tgcgccagga gctcgtcaac acgctggccc acgacgtgcg gaaccccctg
      721 acggcgatcc gggggacgct gaccctgctc ctgacgcggt ccgacaaggt cgatgccgag
      781 accggccgcg agctgctcgg caacgcggac cgccaggcgc gccggctcga gacgctggcg
      841 gaggacctgc tcgagctcgc gcgcctggag aagggacggc tcgatctggc gatccagtac
      901 gtgacgctca gggagacggt cgagcgtggc ctctccttca ccgaccacga gagcgcgtac
      961 cggatcgaca tccccgagga tctccgtctg cgcgccgacc ccggccggct cgagcagatc
     1021 gtcgtcaacc tcgcgtcgaa cgccctgcgg tacggggagg cgccgttcga ggcccgcgcg
     1081 cacgtcgacg gcgatgccgt cgtgctcacc ctccgggacc atgggcccgg catcccggag
     1141 agcgaggccc ggcacctctt cgaccccttc cgtgccgagc agaagaaggg ctcggtcggg
     1201 ctcggcctcg ccatcgtgaa gggcctggcc gaggcccacg gcgggaccgt ccggtacctg
     1261 ccgaacgagc cccgcggcgc ctgcttcgag gtccgcctgc cattgacggg cccacgcgag
     1321 ccggcgaccg tctgaccctc ccgtttgcgg cccgccaggt cggggcggtg aaatgcgtcc
     1381 catggagccg ctcctgctca ccggcaccgc cctcccgctg gaagcgatcg aggcggtcgc
     1441 gcgccgcgga gcgccggtcc gcctcgcgga cgaggcggcg gcggcggtgc gctcgtcgcg
     1501 cgacctggtc gagcagcacc tcggtcgcga ccgcccgatc tacgggatca acaccgggat
     1561 cggcgccctc gccgacacca cgatcccggc gggcgacctc gagcagctcc agcgcaacat
     1621 cgtccgcagc cactcggccg gggtcggcac gctcctgccg gccgaggtcg tccgggcggt
     1681 catccttctc cgcgcccgga ccatggccac gggccgcacg ggcgttcggg tcgaggtgat
     1741 cgaacggctc ttggagctcc tgaaccgggg gatcacgccg gccgtgccga gccggggctc
     1801 ggtcggcgcg agcggtgacc tggcccagct cgcgcacatc ggccagtgcg tgatcggcga
     1861 gggagcggtc ctgcacgggg acggggtgag accggcggcg gaggtcctcc gcgaggaagg
     1921 cctcgagccg ctcgagctcg cgacgaagga aggcctggcg ctcatcaacg ggaccgaggg
     1981 gatggtggcc ctcggggcgc tcgccctcgt ggacctcggt cggttgctcc gggcggcgga
     2041 cgtcgccgcc gcgatgaccg tcgaggcagc gctgggcacg gaccggccgt tcgcggagga
     2101 cctgatggag ctccgaccgc accccggcca ggccgcctcg gcggccaacc tccgagcgat
     2161 ccttcgagac agcccggtga tcgcatcgca tcgagacagc acccacgccg tgcaggaccc
     2221 gtattcgatg cgctgcgcgc cccaggtcca cggggcggca cgcgagacgc acgcgttcgc
     2281 ctccatggtc ctcggtcgcg agctcggcgc ggtcaccgac aaccccgtcg tcctgcccga
     2341 cggccgcgtc gagagcgcag ggaacttcca cggcgagccc ttggcctacg ccctcgacct
     2401 cctggcgatc ggcgccaccg gtctcgcgag catctccgag cgccggacct tctggctcct
     2461 cggaccgggc acgagccggg acctcccccc gttcctctcc acgcaacccg ggctcggctc
     2521 cgggtacatg ctcgcgcagt acacccaggc cgcactcgtg tccgaggcga agggcctgtc
     2581 gcatcccgcg ggcctcgact cgatcccgac gtcggggtcg caggaggatc acgtctcgat
     2641 ggggtggacg tccggcctga agctgcgtga ggtgatcggc cacctccgga gcgtgatcgc
     2701 gatcgaggtg ctctgcgccg cgcaggccct cgacctgcgg cgcccgatcg cacccgcggc
     2761 cggcaccgcc gccgcgctcg cggccgtccg cgagcgcgtt ccattcctcg accaggaccg
     2821 tgacctctcg gtcgacatcg aggcggtgac gggtctggtc gcgggaggcg aactggtcca
     2881 gcgggtcgag gacgcggtgg gtcctctcgc gtgagcgaac ggctaggctg gccatcgtga
     2941 ggacgatccg ggcgccgcgc ggacccgagc ggagctgcaa gggctggccg caggaagccg
     3001 ccctgcggat gctcatgaac aacctcgacc ccgacgtcgc cgaacgcccg caggacctga
     3061 tcgtctacgg cggcacgggg cgcgcggcgc gctcctggga ggccttcgac gcgatcgttc
     3121 gcaccctccg cgacctcgac gacgacgaga ccctcctcgt ccaatccggc aagcccgtcg
     3181 gcgtgttccg gacgcacgaa cacgccccgc gggtcctgat ctccaactcg atgctcgtcc
     3241 cgcgatgggc gacgcacgag accttccgcg acctcgagga ccagggcctc acgatgtacg
     3301 gccagatgac cgccgggtcc tggatctaca tcggcaccca ggggatcctg cagggcacct
     3361 acgagacgct cgcggagctc gcttcccagc ggttcgaccg gacgctccgg ggccggatcg
     3421 tcctcaccgc ggggctcggc gggatgggcg gcgcccagcc cctcgccgtc acgatgaacg
     3481 agggtgtggc cctctgcgtc gaggtcgacc ccgcgcggat cgagcgtcgc atccggaccc
     3541 gctacctcga ccgggcgacc gaggacctcg atgaggcgat cgcatgggcg atcgaggcac
     3601 gagacgccgg gcgggcgctc tcgatcgggc tccgcggcaa tgccgccgaa gtcgtgccgg
     3661 agctcctgcg ccgggacttc ccggccgacg cggtcacgga ccagacctcg gcgcacgatc
     3721 cgctcgacgg ctacgtcccc cacggcctcc cgctcgaccg ggccgacgag ctccgccgtt
     3781 ccgaccccga cacctaccgc aagcgcgccc acgaggcgat ggcggtccac tgcgaggcga
     3841 tggtcgggtt cctcgaccgg ggttcggtgg tcttcgacta cggcaacaac ctccgcgccg
     3901 gcgcgcggga ggggggcttc gagcgtgcct tcgcctaccc aggcttcgtc ccggcgttcg
     3961 tccggccgat gttctgcgag gggaagggcc cgttccggtg ggcggcgctg tccggggacc
     4021 ccgaggacat cgcgacgacc gaccgggcga tcgcggagct cttcccccag gacgagcgcc
     4081 tccaccgatg gctcgggatg gcccgcgagc gcgtggcctt ccaggggctc cccgcgcgga
     4141 tctgctggct cggctacggc gagcggcacc tggccggcct gcgcttcaac gagctcgtcg
     4201 cctccggtgc ggtctcggca cccatcgtca tcggacgcga ccatctcgac agtggctcgg
     4261 tcgcctcgcc ctaccgcgag acggagggca tgcgcgacgg gtccgacgcg atcgcggact
     4321 ggccgatcct caacgcgctc gtgaacacgg ccgcgggtgc ccactgggtg agcgtgcacc
     4381 acggaggcgg ggtggggatc ggctacgcga tccacgccgg gatggtggtg gtcgccgacg
     4441 gcagcgacga cgcggcccga cggctcgagc gcgtgctgac gaccgacccg gggaccggcg
     4501 tgatgcggca tgccgacgcc ggctacgacg aggcgctccg cgtcgcgcga gaccgcggcg
     4561 tccgcctccc gatgctcgag ggcgagtgac ggacgacccg aactggcccc gctcctccgc
     4621 ctggttgagc ggcgaccgtg accgggcccg cacggcggcg atcgcggtcc tcggcgttcc
     4681 gatccggcgc ggctcgatct ccggcggccg gttcgacctc gctccgggcg ccgtgcggag
     4741 cgcgctcggc cgcttcagca cctgggacgg cgaacgggat ctcggggcgg tgcgcgcggt
     4801 cgacctcggg gacctcgacg tcgcgtcgca gacggtcgag gaggcccgcc acacgatcga
     4861 gcgatcgatc ggcgaggccc caggaagggc tccgctcgtc ctgctcggcg gagacaactc
     4921 gatcacgtgg gccgcggcgt ccgcgctcag cgcagagggc ggacccgtcg gcctcatcac
     4981 gctcgacgcc caccacgacc tccggggaac ggaccacggc atatcgaacg gcgccccggt
     5041 ccgtgcgctg ctcgaagacg gaaccatcgc cggagggaac gtctggcaga tcggcatcca
     5101 gccgttcgcc aactcgcccg cctacgcggc ggtcgccacc gaccacggta tccacgtgac
     5161 ccggccagag gaggtccgcg cccgcgggat cggatccgtc atggatgatg cgctcgcgga
     5221 cctggacgcg agatgcgacg tcatctacgt agacctcgac gtcgacgtgc tcgaccgggc
     5281 gttcgccccc gcctgccccg gcgcacgtcc gggggggctg ctcccctggg aagtccacga
     5341 ggcggcccgt ctcgccggag cccatccgaa ggtgcgggcg atggacctcg tggagatcga
     5401 ccccgagcgg gacgtggcgg acgtcaccgt gctcaacgcg gcgcgctgcc tgctcggctt
     5461 cgccgccggg gtcgccacga ggaagtcagc ggactaaccg gaacgatccg gggaatagtc
     5521 ggccgtacag acggcccacc acaggaggtt ccgcccatgc ctcgacggct caggacgctt
     5581 ggtgtcatcg cgatcgtcgc cctcggactg accgcgacgc ccgcggccgg cgatcacacc
     5641 gatccccggg atccactctc ccccaccagc cccgggctcc cgggcaacgg catcgtccag
     5701 ggggcgggac ggtgggagca catccgcaac ttcccgccga accccggcag cgacctggag
     5761 ttcttccgca agggcggaga catctacgcc tcgacgggca ccctcgggca gggtgaggcc
     5821 ggccacgtcg gtcagcggat catccgcctc acgcacaacg gcgccgtgaa ccccacgtgg
     5881 gtcgccgacc acggctcggc cgcatgccgc accgcgaacc ccaccggcac gctcggcctc
     5941 cagcacgacg tccagatcac cccgaagcac aacccgcagc tcgtgatcga cgcgaccgac
     6001 gcgaccggcc gctgtcacga tcccgcgggc ggcgggctcg agttcgtcga catcagcggg
     6061 atcggcccga agagccggtt ccagccgcgg gagatccacc tgacccgcca cgccgggctg
     6121 tcgcacaccg tcaccgtgga cacggtcagg ccgtggatcg tctacaacag cacgtcggac
     6181 tcgagcgggg cgaaccgccc ctggatcgac gtcctcgaca tccggacctg cctcgggcgc
     6241 accggtgaca cgatcgcgca gaagcgcgcg cggtgccgcc cgctcgtgta ccggatcccg
     6301 ttcgagcccg agtggtcgca gcgcgtccgg ccgaacggcg agctcgagcc cggctcggag
     6361 agctcctgcc acgacatcac ctcggtcggc tcccgcatct actgcgccgg cctgaacgcc
     6421 accctcatct tcgacacgtc gaagatgagg gtggcgttca ggccggcgca gtagatgcgg
     6481 gagccgaccg aggtgatgtc gtggcaggag ctctccgagc cgggctcgag ctcgccg
//
LOCUS       DATLGG010000284         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_11679, whole genome shotgun sequence.
ACCESSION   DATLGG010000284 DATLGG010000000
VERSION     DATLGG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11679"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VM638_08095"
     CDS             complement(<1..750)
                     /locus_tag="VM638_08095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVM12419.1"
                     /translation="MSVIERRKVVYEGTRIPTPIPTVLQPNPEEVPLEDLMRINFGPN
                     HPSTHGVLRLVVDLNGEHVVGVSAVIGYLHTGFEKTMEQKTWWKAITYSPRIDYLSFQ
                     NNELVFVLATEKLLQLPIPRKATWMRMCMAELNRIHSHLVWLGTGALELGAISLFWYA
                     FRERDLVLDLFEMVTGYRMHTRYFQVGGLAEDIPPGFYAEAWKFVERMPKAVDEYEAL
                     LTDNTIWRERTEGVGLLSADDAIALGQSGPML"
     gene            complement(753..1301)
                     /locus_tag="VM638_08100"
     CDS             complement(753..1301)
                     /locus_tag="VM638_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVM12420.1"
                     /translation="MAFEAVPGLVEPREALGETTLVVDPARLLEAAQHLRDEEGFNFL
                     ADIAAADYLGWGHHGVAGYIGTAGGRDINYPGSQGYAKRPDPKPKRFSVSYHLLELSD
                     TPRRVRLQVWLDDGEPIPSVVGIWPTADWHEREAWDMMGIRIEGHPNMRRILMDDDWE
                     GHPLRKDYPIGGEPVRFSEEEA"
     gene            complement(1304..1792)
                     /locus_tag="VM638_08105"
     CDS             complement(1304..1792)
                     /locus_tag="VM638_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HVM12421.1"
                     /translation="MENLEDAIGLTTLQKAVAWAQTNSTWPATFGLACCAIEMMSIVS
                     SRYDIARFGMERFSSSPRQADLMIVSGRLSHKMAAPMRQIYDQMLEPKWVIAMGACAS
                     SAGMFNNYAVLQGVDKIVPVDVYVPGCPPRPEALMEGIVRLHEAVNAGVPPAYQIRGV
                     GE"
     gene            complement(1903..2265)
                     /gene="ndhC"
                     /locus_tag="VM638_08110"
     CDS             complement(1903..2265)
                     /gene="ndhC"
                     /locus_tag="VM638_08110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVM12422.1"
                     /translation="MLADYFPFLVYALFAAAIPASMIAFSFLFATSPKRRVRARLLPF
                     ESGVSQGAPTQQRFTISFYLTAMLFILFDIEIVFLYPLAIVLESLGWFGLVEFLVFIG
                     ILGVAYVYIWRKGALEWR"
     gene            2399..2812
                     /gene="erpA"
                     /locus_tag="VM638_08115"
     CDS             2399..2812
                     /gene="erpA"
                     /locus_tag="VM638_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HVM12423.1"
                     /translation="MEIVERPTSLVSLTATAADKIKQLMAEDADADSMVLRVAIQGGG
                     CSGFQYGLGFDAGPVEDDVVDEQHGVTVVIDPFSAPYLRGATIDFLDGLQESGFKIDN
                     PNAAASCGCGHSFTVEDGAEPGDAHAGGCGSGCGC"
     gene            complement(2825..3391)
                     /locus_tag="VM638_08120"
     CDS             complement(2825..3391)
                     /locus_tag="VM638_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12424.1"
                     /translation="MLRNRTRPALLLLAVVLAAGCGGSDGDGGGDRAEGVAFAVYADD
                     AIGDRADDGWLRCGAPRAACPPNFLQPTGSYTYEPPGEPALGPRHTAGARTEAAGGAW
                     SVSIELTGAGAKAFGDLTKRLAQEGKEEARPRSFAVVVGTEIVAYTTVHYLEFPEGIQ
                     TTSLELFATGEDDAKAIVERINAAVAAR"
     gene            3399..>3808
                     /locus_tag="VM638_08125"
     CDS             3399..>3808
                     /locus_tag="VM638_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVM12425.1"
                     /translation="MSDSGERGSGLRVAVVGSGPAGFYAAAALLSSDLGAEVDVIERL
                     PTPWGLVRLGVAPDHPKIKAVSRAFERTAQQPGFRFLGNVEVGRDVTHDELARLYDAV
                     LYAVGAQTDNRLGIPGEDLPGSWAATELVAWYNG"
BASE COUNT          581 a         1314 c         1333 g          580 t
ORIGIN      
        1 cagcatcggg cccgactgcc cgagcgcgat cgcgtcgtcg gcggagagga ggccgacgcc
       61 ctccgtccgc tcgcgccaga tcgtgttgtc ggtgaggagc gcctcgtact cgtcgaccgc
      121 cttcggcatc cgctcgacga acttccaggc ctcggcgtag aagccgggcg ggatgtcctc
      181 ggcgagaccg ccgacctgga agtaccgcgt gtgcatgcgg taccccgtga ccatctcgaa
      241 gaggtcgagc acgaggtcgc gctcgcggaa ggcgtaccag aagagcgaga tcgcaccgag
      301 ctcgagcgcg ccggtgccga gccagacgag gtgcgagtgg atcctgttca gctccgccat
      361 gcacatccgc atccacgtcg ccttgcgcgg gatcggaagc tgcagcagct tctccgtcgc
      421 gaggacgaac acgagctcgt tgttctggaa cgagaggtag tcgatccgcg gcgagtaggt
      481 gatcgccttc caccacgtct tctgctccat cgtcttctcg aagccggtgt ggaggtagcc
      541 gatcaccgcc gagacgccga cgacgtgctc gccgttcagg tcgacgacga gccggagcac
      601 gccgtgcgtg gacgggtggt tcggcccgaa gttgatccgc atcaggtcct cgagcggcac
      661 ctcctccggg ttcggctgga ggacggtggg gatcggcgtc gggatgcgcg tcccctcgta
      721 gacgaccttg cgacgctcga tcaccgacac ggctacgcct cctcctcgga gaagcggacg
      781 ggctcgccgc cgatcgggta gtccttgcgc agcgggtggc cctcccagtc gtcgtccatg
      841 aggatgcggc gcatgttcgg gtggccctcg atgcggatcc ccatcatgtc ccaggcctcc
      901 cgctcgtgcc agtcggcggt gggccagata ccgacgacgc tcgggatcgg ctctccgtcg
      961 tcgagccaga cctggagacg cacgcgccgc ggcgtgtccg agagctcgag caggtggtag
     1021 ctgaccgaga accgcttcgg cttcgggtcc gggcgcttcg cgtacccctg cgagcccggg
     1081 tagttgatgt cgcggccgcc ggcggtgccg atgtagccgg cgacgccgtg gtgtccccag
     1141 ccgaggtagt cggcggcggc gatgtccgcg aggaagttga agccctcctc gtcccggagg
     1201 tgctgggccg cctcgaggag ccgtgccggg tcgacgacga gcgtcgtctc gccgagcgcc
     1261 tcgcgcggct cgacgagacc gggcaccgcc tcgaacgcca ccgctactcc ccgactccgc
     1321 ggatctggta cgcgggcggc accccggcgt tgacggcctc gtggaggcgg acgatcccct
     1381 ccatcagcgc ctccggccgc ggagggcaac ccgggacgta cacgtccacg gggacgatct
     1441 tgtcgacacc ctggaggacg gcgtagttgt tgaacatccc ggcggagctc gcgcaggcgc
     1501 ccatcgcgat cacccacttg ggctcgagca tctggtcgta gatctgcctc atcggcgccg
     1561 ccatcttgtg cgagaggcgg cccgacacga tcatcaggtc ggcctgccgc ggcgaggagc
     1621 tgaaacgctc catcccgaag cgggcgatgt cgtagcgcga cgagacgatc gacatcatct
     1681 cgatcgcgca gcaggcgaga ccgaaggtcg cgggccacgt ggagttcgtc tgcgcccacg
     1741 ccacggcctt ctggagcgtc gtcaggccga tcgcgtcttc caggttctcg atctcctgct
     1801 cgaacttccc cgggcccttc gtcggccaga gcaggcgctc gctcttgagc cccgcacgtg
     1861 cgagcggccg gttcgagacc cgcttcgggt cgatcgattt gtctatcgcc actcgagcgc
     1921 gcccttccgc cagatgtaga cgtaggcgac gccgaggatc ccgatgaaga cgaggaactc
     1981 gacgagtccg aaccagccga gcgactcgag cacgatcgcg agcgggtaga ggaagacgat
     2041 ctcgatgtcg aagaggatga agagcatcgc cgtcaggtag aagctgatgg tgaagcgctg
     2101 ttgcgtcggc gctccctggg agacgccgga ctcgaacggg agcagccgcg cccgcacgcg
     2161 acgcttcggg ctcgtcgcga acaggaacga gaacgcaatc atggacgccg gaatcgcggc
     2221 cgcgaagagc gcgtagacca ggaagggaaa gtagtccgcg agcatcggct tcgcgcgaac
     2281 gctagcaagg tgcaggtgcg tcgcacgaag gaaccgcgaa accgcttgcg gacgcggaag
     2341 aaggtgtcac gaagttgcgc tttccggggg gttccgtggg cgttgctacc atgcccgcat
     2401 ggagatcgtc gagcgcccca cgagcctcgt ttcgctcacc gcgaccgcgg ccgacaagat
     2461 caagcagctg atggcggagg acgccgatgc cgactcgatg gtcctccgcg tcgcgatcca
     2521 gggcggcggg tgctccggct tccagtacgg cctcggcttc gatgcaggcc ccgtcgagga
     2581 cgacgtggtc gacgagcagc acggcgtcac cgtcgtgatc gaccccttca gcgccccgta
     2641 cctccgcggc gcgacgatcg acttcctcga cggccttcag gagtccgggt tcaagatcga
     2701 caacccgaac gccgccgcgt cctgcggctg cgggcactcc ttcacggtcg aggacggcgc
     2761 cgagcccggc gacgcccatg cgggcggctg cggctccggc tgcggctgct agcccgctcg
     2821 cggatcagcg cgcggcgacc gccgcgttga tgcgttcgac gatcgctttc gcgtcgtcct
     2881 cgcccgtcgc gaagagctcg agcgacgtcg tctggatccc ttccgggaac tcgaggtagt
     2941 ggaccgtcgt gtaggcgacg atctccgtac cgacgacgac cgcgaagctg cggggccgcg
     3001 cctcctcctt cccctcctgc gcgagcctct tcgtcagatc gccgaaggcc ttcgcgcctg
     3061 cgccggtcag ctcgatcgag accgaccatg ccccaccggc ggcttcggtg cgcgcaccgg
     3121 ccgtgtgccg cggcccgagc gcgggctcgc ccggcggctc gtaggtgtac gagccggtcg
     3181 gctgcaggaa gttcggcggg cacgcggcgc gcggggcgcc gcagcgcagc catccgtcgt
     3241 cggcgcggtc gccgatcgcg tcgtcggcgt agacggcgaa ggcgactccc tccgcgcggt
     3301 cgccgccgcc atcgccgtcg ctcccgccgc agccggcggc gagcacgacc gcgagcagga
     3361 gcagtgcggg ccgggtccgg ttccgtagca tcgccggcat gagtgatagc ggagagcgcg
     3421 gctccgggct ccgggtcgca gtcgtcggat ccggacccgc ggggttctac gccgcagcgg
     3481 cgcttctctc aagcgacctc ggcgccgagg tcgacgtgat cgagcggctc ccgacgccgt
     3541 ggggcctcgt gcggttgggc gtcgcgccgg accaccccaa gatcaaggcg gtctcgcgcg
     3601 cgttcgagcg gacggcgcag cagccggggt ttcgcttcct cggcaacgtc gaggtcgggc
     3661 gcgacgtgac gcacgacgaa ctggcgcggc tctacgacgc cgtcctctac gccgtcggcg
     3721 cgcagaccga caaccgcctc gggatcccgg gcgaggacct ccccgggtcg tgggccgcga
     3781 cggagctcgt cgcctggtac aacggcca
//
LOCUS       DATLGG010000285         5245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_200916, whole genome shotgun sequence.
ACCESSION   DATLGG010000285 DATLGG010000000
VERSION     DATLGG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200916"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VM638_08130"
     CDS             complement(<1..291)
                     /locus_tag="VM638_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12426.1"
                     /translation="MAKDVQPPHVEEHPPAAEQPRDDVRTPPARPPGQRVDLLLNRIV
                     HFLVALFLFVLAIQLMKKGAQAIAPRLQGTFPFDNGVSTLGLGWLGAYLVLSG"
     gene            387..2813
                     /gene="leuS"
                     /locus_tag="VM638_08135"
     CDS             387..2813
                     /gene="leuS"
                     /locus_tag="VM638_08135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HVM12427.1"
                     /translation="MRPYDPSEIEPRWVATWEREDLYRAREDDPRPTFYALDMFPYPS
                     GDLHMGHAEAFAVGDAIARSKAMRGYNVLHPIGWDAFGLPAENAAIKRGIHPKEWTYQ
                     NIDIQRQSFRRYGISFDLSRQFNTCDPEYYRWTQWLFLRFFERGLAYRKLAPANWCPN
                     DRTVLANEQVVNGRCERCGAEVVRKDLTQWFFRVTDYAQELLDDMAMLPGWQDRVLAM
                     QTNWIGRSEGAEVEFEIAETGDRVTVFTTRPDTLWGVTFFVFAPEHPLVEALAQAGGS
                     AEPARALRDRLRSTPLTDREQAESREGVALGVHAVNPVSGEKIPCFVAPYVLMEYGTG
                     AVMGVPGHDQRDFEFAREHGLPIRVVIRPEGEDLRPEEMTEAYAHDGVMVDSGPFDGT
                     PATEAVAAVIAWLEEQGKGRAAISYRLRDWLISRQRYWGCPIPIVHCEDHGEVAVPDE
                     DLPVLLPDDVDFSPTGESPLARHEGFVDATCPTCGKPARRETDTMDTFVDSSWYYLRY
                     CSPDEDARPFDPERVAKWMPVDQYTGGIEHAILHLLYSRFFTKVLADMGMVPFREPFP
                     NLLNQGMVIMQGAKMSKSKGNLVEPMPLIEEHGADTIRVTMLFAAPVEDDVDWATVQP
                     AGVKSWLGRVWRAVHEAAERGGEDPDELRRSTHRTIRDVSGLYDRFRFNVAVARLMEL
                     TNEMRRTLDGGGAALEAARSLVLMLAPMAPFITEELWRGPLGGEGSVHRAAWPGYEDS
                     LAKRETATLVVQVNGKVRDTIEVAPDISEDEAVRLARASEKVARSLGDAEIARVIARP
                     PKLVNLVTGS"
     gene            2851..3795
                     /locus_tag="VM638_08140"
     CDS             2851..3795
                     /locus_tag="VM638_08140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVM12428.1"
                     /translation="MRRSLFRRRWWRRAARLVPLDFPVFVVIAAALVMLGLTFRFGLQ
                     GGTATAPPAEPPPTTLPPFAGSVGPGQLAVGPEEIERTLRRLVRIGRPVRCGAGNQPL
                     VALTFDDGPGPRTADLLRILAVNSAPATFFFVGDRLGYPGAPTIAAMGPMHAIGNHTW
                     SHASLRRLRPRDRRSEVLRAQRALERAAESPIHLFRPPYGHRDPALDRMVSRLGLVQV
                     LWSSTSGDDRPVTARALMRKLRGELQPGSIVLMHEHEITVSTLPRVLELLRDRGLRPV
                     TVAELLALDPPTRRQLKTGLCPRGTPDAISAEAGGGRP"
     gene            complement(3764..4414)
                     /locus_tag="VM638_08145"
     CDS             complement(3764..4414)
                     /locus_tag="VM638_08145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HVM12429.1"
                     /translation="MDIVAPKVEDYLRGLFATDGHPVAEEMERVAEERDFPIVGPLVG
                     RMLELFARSVGARRILELGSGYGYSAYWFARAVEPGGEVHMTEGDPENQREALAYLER
                     AGLADRVHSHVGDAIELMDRLEGEFDVIFCDIDKPAYPDAWRKARERLRPGGLYLCDN
                     VLWSGRVADEGPAEGDGGVTDAIREHNALVAADADYDTTIVPLRDGVMVARRRLPR"
     gene            complement(4419..>5245)
                     /locus_tag="VM638_08150"
     CDS             complement(4419..>5245)
                     /locus_tag="VM638_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVM12430.1"
                     /translation="LTLAVLRMLVAGAALVIALALAGSLRRPPRDGRLVLASVALAAT
                     LIVQFVGTDLATATQAALLTTTTPLFLVPFAWAGLRERPTWPTVGGILAGMAGVVVAV
                     GPGAGRAGSRWGPLLLLASAMLWATYTVASARAARRDGPLVTVTWATIGAIPPLALAS
                     LLEVDRWGSASLTHLPTLGAIAYLGLAASAGAWYLWNRGVAGLPAAVAGAFFFAQPVV
                     GGILAWLLLGERPTVRLGLGALLIVVGVLLALRATSRQAPPDEGQTDVPPDRDRVG"
BASE COUNT          760 a         1869 c         1910 g          706 t
ORIGIN      
        1 ccccgagagc acgaggtagg cgcccagcca gccgaggccg agcgtgctga cgccgttgtc
       61 gaaggggaag gtgccctgga gccgtggcgc gatcgcctgg gcccccttct tcatcagctg
      121 gatcgccagc acgaacagga agagcgcgac gaggaagtgc acgatcctgt tcaggagcag
      181 gtcgacccgc tgccccggtg gtcgcgcggg gggcgtgcgg acgtcgtccc gcggctgctc
      241 ggccgcgggc gggtgctcct cgacgtgggg gggctggacg tccttggcca tggatgggaa
      301 ccgggccgcc gctccggaac cgctaccata tcggcaggtc agcaagggtg tccacgttca
      361 cgtcgccgtc gatcgtcgag gttcgcatgc gtccgtacga cccgagcgag atcgagcccc
      421 ggtgggtcgc cacctgggag cgggaggatc tgtaccgcgc ccgcgaagac gacccgcgcc
      481 cgaccttcta cgcgctcgac atgttcccgt acccctccgg ggacctccac atgggccacg
      541 ccgaggcgtt cgcggtcggc gacgcgatcg cacggtcgaa ggcgatgcgc ggctacaacg
      601 tgctccaccc gatcggttgg gacgccttcg gcctgccggc ggagaacgcc gcgatcaagc
      661 gcggtatcca cccgaaggaa tggacctatc agaacatcga catccagcgg cagtcgttcc
      721 gccggtacgg gatctcgttc gacctgagcc ggcagttcaa cacgtgcgac ccggagtact
      781 accggtggac gcagtggctg ttcctgcgat tcttcgagcg cgggctcgcc taccgcaagc
      841 tcgcccccgc gaactggtgc ccgaacgacc ggacggtcct cgcgaacgag caggtcgtca
      901 acgggcgctg cgagcgctgc ggcgccgagg tcgtccgcaa ggacctgacg cagtggttct
      961 tccgcgtcac cgactacgcg caggagctgc tcgacgacat ggcgatgctc ccgggctggc
     1021 aggaccgcgt gctcgcgatg cagacgaact ggatcggccg ctccgagggc gccgaggtgg
     1081 agttcgagat cgccgagacc ggtgaccgcg tcaccgtgtt cacgacgcgg cccgacacgc
     1141 tgtggggggt gaccttcttc gtcttcgcgc ccgagcaccc gctcgtcgag gcgctcgcgc
     1201 aggcgggtgg atccgccgag ccggcacgcg cgctccggga ccggcttcgg tcgacgccgc
     1261 tgaccgatcg cgagcaggcc gagtcgcgcg agggagtcgc gctcggggtg cacgcggtga
     1321 accccgtgag cggcgagaag atcccgtgct tcgtcgcgcc gtacgtgctg atggagtacg
     1381 ggacgggcgc ggtgatgggc gtcccggggc acgaccagcg ggacttcgag ttcgcgcgcg
     1441 agcacggttt gccgatccgc gtcgtgatcc gccccgaggg cgaggacctg cgccccgagg
     1501 agatgaccga ggcgtacgcg catgacggcg tcatggtcga ctccggcccc ttcgacggga
     1561 cccccgccac ggaggccgtc gccgcggtga tcgcgtggct cgaggagcag gggaagggga
     1621 gggcggcgat ctcgtaccgg ctccgcgact ggctgatctc gcgccagcgg tactggggct
     1681 gtccgatccc gatcgtccac tgcgaggacc acggcgaggt cgcggtgccc gacgaggacc
     1741 ttccggtcct gctccccgac gacgtcgact tctcgccgac cggcgagtcg ccgctcgcgc
     1801 ggcacgaggg gttcgtcgat gcgacctgcc cgacgtgcgg gaagcccgcc cggcgggaga
     1861 ccgacacgat ggacaccttc gtcgactcct cctggtacta cctccggtac tgctcacccg
     1921 acgaggacgc gcgcccgttc gatcccgagc gcgtcgcgaa gtggatgccg gtcgaccagt
     1981 acaccggcgg gatcgagcac gcgatcctgc acctgctcta ctcgcgcttc ttcacgaagg
     2041 tcctcgcgga catggggatg gtgccgttcc gcgagccgtt cccgaacctg ctgaaccagg
     2101 ggatggtgat catgcagggt gccaagatga gcaagagcaa gggcaacctc gtcgagccga
     2161 tgccactgat cgaggagcac ggcgccgaca cgatccgcgt gacgatgctc ttcgccgcgc
     2221 cggtcgagga cgacgtcgac tgggcgacgg tccagccggc cggtgtgaag tcgtggctcg
     2281 ggcgggtgtg gcgcgcggtg cacgaggcgg ccgagcgtgg cggtgaggac ccggacgagc
     2341 tccggcggtc tacccaccgg acgatccgcg acgtgagcgg cctctacgac cggttccggt
     2401 tcaacgtcgc ggtcgcgcgc ctgatggagc tgacgaacga gatgcggcgg acgctcgacg
     2461 gcggaggcgc tgcgctcgag gcggcgcgct cgctcgtgct gatgctcgcc ccgatggccc
     2521 ccttcatcac cgaagagctg tggcggggtc ccctcggcgg ggaggggagc gtccaccgcg
     2581 ccgcctggcc cgggtacgag gactcgctgg cgaagcggga gacggcgacc ctcgtggtgc
     2641 aggtgaacgg gaaggtccgg gacacgatcg aggtcgcccc cgacatctcg gaggacgagg
     2701 ccgtccgcct cgcgcgcgcc tcggagaagg tcgctcgctc gctcggcgac gccgagatcg
     2761 cacgtgtgat cgcccgtccc ccgaagctgg tgaacctcgt cacgggctcc tagctcccgg
     2821 aagagcggcg gcgggtacca tcgcagcggg atgaggaggt cactgttccg tcggcgatgg
     2881 tggcgtcgcg cggcccgcct cgtcccgctg gacttcccgg tgttcgtcgt gatcgctgcc
     2941 gccctggtga tgctcggcct cacgttccgc ttcgggctcc aggggggcac ggcgacggcg
     3001 ccgcctgcgg agcccccgcc cacgaccctg ccgcccttcg cgggctccgt cggtcccggg
     3061 cagctcgcgg tcgggcccga ggagatcgag cggacgctac ggcgtctcgt ccggatcggc
     3121 cggccggtcc gctgcggcgc gggcaaccaa ccgctcgtcg cgctcacgtt cgacgacggt
     3181 cccggccccc gcaccgcgga cctgctccgg atcctggccg tgaacagcgc cccggcgacc
     3241 ttcttcttcg tcggcgaccg cctcggctac cccggggccc cgacgatcgc ggcgatggga
     3301 ccgatgcacg cgatcggcaa ccacacgtgg agccatgcca gcctgcggcg gctgcggccg
     3361 cgggaccggc gctccgaggt cctccgggcc cagcgggcgc tcgagcgagc ggcggagtcg
     3421 ccgatccatc tgttccggcc tccgtacggc caccgcgacc cggccctcga ccggatggtc
     3481 tcccggctcg gcctcgtcca ggtgctgtgg agctccacct ccggcgacga tcgtccggtg
     3541 acagcccgtg cgctgatgcg gaagctccgc ggcgagcttc agccggggtc gatcgtgctc
     3601 atgcacgagc acgagatcac cgtgagcacc ctcccgcggg tgctcgagct cctgcgggac
     3661 cgggggctgc gcccggtcac cgtcgccgag ctgctggcgc tcgacccgcc gacccgacgg
     3721 cagctgaaga cgggcctctg cccgcggggg acgccggacg cgatcagcgc ggaagccggc
     3781 ggcgggcgac catgaccccg tcgcggagcg gaacgatcgt cgtgtcgtag tcggcgtcgg
     3841 cggcgacgag cgcgttgtgc tcgcggatcg cgtcggtcac ccctccgtcg ccttccgccg
     3901 gcccctcatc ggcgacgcgg ccggaccaca gcacgttgtc gcagaggtac aacccccccg
     3961 gacgcagccg ttcccgcgcc ttccgccagg cgtccgggta cgcaggcttg tcgatgtcgc
     4021 agaagatgac gtcgaactcc ccttcgagac ggtccatcag ctcgatcgcg tcgccgacgt
     4081 gggagtgcac gcggtcggcg aggcccgccc gctcgaggta ggcgagggct tcccgctggt
     4141 tctcgggatc gccttcggtc atgtgcacct ccccgccggg ttcgacggcg cgcgcgaacc
     4201 agtaggcgga gtacccgtac ccggagccga gctcgaggat ccggcgcgcg ccgacgctgc
     4261 gcgcgaagag ctcgagcatc cgcccgacga gcggcccgac gatcgggaag tcccgctcct
     4321 cggcgacccg ctccatctcc tcggcgaccg gatgcccgtc cgtcgcgaag agcccgcgga
     4381 ggtagtcctc gaccttcgga gcgacgatgt ccatgcggtc agcctacgcg gtctcggtcg
     4441 ggcggaacgt cggtctgacc ctcgtccggg ggcgcctgcc tcgacgtggc gcggagggcg
     4501 agcaggaccc cgacgacgat caggagtgcc ccgaggccga gtcggaccgt gggtcgctcg
     4561 ccgagcagga gccacgcgag gatcccgccg accaccggct gggcgaagaa gaacgcgccc
     4621 gccaccgccg cgggaagccc ggcgaccccg cggttccaca ggtaccaggc acccgccgac
     4681 gcggcgaggc ccaggtaggc gatcgcgccg agcgtcggca ggtgcgtgag gctcgcgctg
     4741 ccccagcgat ccacctcgag gagcgaagcg agggcaagcg gcgggatcgc gccgatcgtc
     4801 gcccatgtga ccgtgacgag cgggccgtcg cgacgagccg ctcgagcgga ggcgaccgtg
     4861 taggtcgccc acagcatcgc cgacgcgagg aggaggagcg gcccccaacg ggaccctgcg
     4921 cgccccgcgc cgggaccgac cgcgaccacc accccggcca tccccgcgag gatcccgccc
     4981 accgtcggcc acgtcggccg ctcgcgcaga ccggcccacg cgaacggcac gaggaacagc
     5041 ggggtcgtcg tcgtcagcag cgctgcctgg gtcgcggtcg cgaggtcggt cccgacgaac
     5101 tgcacgatca gggtcgcggc gagcgcgacg gaagcgagca cgaggcggcc gtcgcgcggt
     5161 gggcgccgga gcgatcccgc gagggcgagc gcgatgacga gcgcggcgcc cgcgacgagc
     5221 atgcgcagca ccgcgagggt gagcg
//
LOCUS       DATLGG010000286         4047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_173819, whole genome shotgun sequence.
ACCESSION   DATLGG010000286 DATLGG010000000
VERSION     DATLGG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173819"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VM638_08155"
     CDS             complement(<1..203)
                     /locus_tag="VM638_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HVM12431.1"
                     /translation="MNRLLYVCLDGLGDDPVEAFDGRTPLEAARTPVLDGLAARGRQG
                     LVQTVGKGIAPESDIAVFAILGY"
     gene            complement(200..970)
                     /gene="tpiA"
                     /locus_tag="VM638_08160"
     CDS             complement(200..970)
                     /gene="tpiA"
                     /locus_tag="VM638_08160"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVM12432.1"
                     /translation="MRKPIVAANWKMHKTHLEAIQTVQKLSYLLDADDTKRVEVVVCP
                     PFTALRSVQTIIESDRLTFGLGAQNVSPEEKGAFTGEIAPGMLEALGCSHVIVGHSER
                     RELFGETDEQVARKVRAVLAKGLTPILCVGETAEERDAGTTREKVVGQIRAGIGKLPS
                     EEARRIVVAYEPIWAIGTGRAATPADAGEAVAWIREAIEEAHGREVAEAVRVQYGGSV
                     KAGNIRDFAAHPEIDGALVGGASLDPEEFALIVKGVAS"
     gene            complement(971..2158)
                     /locus_tag="VM638_08165"
     CDS             complement(971..2158)
                     /locus_tag="VM638_08165"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVM12433.1"
                     /translation="MGRLRLIDDLGDLRGRRVLVRVDFNVPLEAGKVTDDLRIAAALP
                     TIERLRDAGAALVLASHLGRPKGQPRGDLRLDPVAARLQDLGGFPVTKLDEVRGGTVE
                     AACRALGPGDVALIENLRFDPGEEANDPGFAEALARLADAYVDDAFGAAHRSHASVVG
                     VAERLPSAAGLLMQREVEVLSRLLTDPEEPFVAILGGAKVSDKLGVVGSLLDRVDALL
                     IGGAMAFTFIAAQGGEIGSSLVERDRVEEVRATLAEAERRGVLILLPEDVVAATEVSA
                     SARKETVRADAVPRGLMGLDVGPRTVEEFARVLSDAKTVLWNGPMGVFELEPFSLGTR
                     GVAQAVAGIDAFTVVGGGDSVAAIRGLGFEGSVDHLSTGGGASLEFLEGRDLPGIAVL
                     MEG"
     gene            complement(2159..3166)
                     /gene="gap"
                     /locus_tag="VM638_08170"
     CDS             complement(2159..3166)
                     /gene="gap"
                     /locus_tag="VM638_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017582648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVM12434.1"
                     /translation="MAIKVGINGFGRIGRNFFRAAKERGADFDLVAVNDITDAPTLAH
                     LLKYDSVLGNLDSEIKATDEGISVDGEEFRVLAERDPGNLPWKELGVDLVIESTGLFT
                     NREKAELHLSAGAKKVIISAPAKGEDITLVMGVNDGEYDPNEHHVISNASCTTNSVVP
                     MSKVLRDSFGIDRALMTTIHAYTNDQSILDLPHKDLRRARAAAVNIIPTSTGAAKATS
                     LAIPELKGRMDGMAMRVPVPDGSVTDLVAILERDVTVDEVNEAFRTAASDGPLAPYLR
                     YTEDPIVSSDIVGDPHSCIFDAQSTMAMGNLVKVVGWYDNEWGYSNRLVDLARLVGST
                     L"
     gene            complement(3246..>4047)
                     /gene="rapZ"
                     /locus_tag="VM638_08175"
     CDS             complement(3246..>4047)
                     /gene="rapZ"
                     /locus_tag="VM638_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HVM12435.1"
                     /translation="KHLEDLGYFVVDNLPPALIGKMAELVLTAGEPSRVAIVADARGG
                     VFFTDLAAALQDLHERRLPYRILFLEANDDALVNRFEATRRRHPLAPGDRVVEGIRKE
                     RLMMETLRGEADLIIDTSQLTPHQLRERIRDGFARDRPGAGLQVQVVSFGYKYGIPRD
                     ADLVLDVRFLPNPHWIDELRPLPGTADAVRGYVTDQPAYAEFMERLRGLLEFVVPGYV
                     AEGKSYLTLAVGCTGGRHRSVVVAEALERWFGEQGLDARAKHRDLDRP"
BASE COUNT          559 a         1477 c         1400 g          611 t
ORIGIN      
        1 tcgtagccga ggatcgcgaa gacggcgatg tcggattcgg gagcgatccc cttcccgacc
       61 gtctgaacca ggccctgccg gccacgcgcg gcgagaccat ccaggacggg cgtccgcgcc
      121 gcctcgagcg gcgtgcggcc gtcgaaggcc tcgaccgggt cgtcgccgag tccgtcgagg
      181 cagacgtaga gcagtcggtt cacgacgcca cccccttgac gatgagggcg aactcctcgg
      241 ggtcgaggct ggcgcccccg acgagcgccc cgtcgatctc ggggtgcgcg gcgaagtcgc
      301 ggatgttccc cgccttcacg cttccgccgt actgcacgcg gaccgcctcc gcgacctcgc
      361 ggccgtgcgc ctcctcgatc gcctcgcgga tccaggcgac ggcctccccg gcgtcggccg
      421 gggtcgcggc ccgaccggtc ccgatcgccc agatcggctc gtaggcgacg acgatccggc
      481 gtgcctcctc ggatgggagc ttcccgatcc cggcccggat ctgcccgacg accttctcgc
      541 gggtcgtccc ggcgtcgcgc tcctccgccg tctccccgac gcacaggatc ggcgtcagcc
      601 ccttcgcgag caccgcgcgg accttacggg cgacctgctc gtccgtctcg ccgaacagct
      661 cccggcgctc tgagtggccg acgatgacgt gcgagcagcc gagggcctcg agcatcccgg
      721 gcgcgatctc ccccgtgaag gcgcccttct cctcggggct cacgttctgg gcgcccaggc
      781 cgaaggtcag ccggtcgctc tcgatgatgg tctgcacgct gcgcagcgcc gtgaagggtg
      841 ggcagacgac gacctcgacc cgcttcgtgt cgtccgcgtc cagcaggtac gagagcttct
      901 ggacggtctg gatcgcctcg aggtgcgtct tgtgcatctt ccagttcgcg gccacgatcg
      961 gctttcgcat ctatccctcc atcaacacgg cgatcccggg caggtcccgt ccctcgagga
     1021 actccaggga ggctcccccg cccgtggaca ggtggtcgac ggacccctcg aacccgagcc
     1081 ccctgatcgc cgcgacgctg tcgccgccgc cgacgaccgt gaaggcgtcg atccccgcga
     1141 cggcctgcgc cacgccgcgc gtccccagcg agaacggctc gagctcgaag acgcccatcg
     1201 gtccgttcca caggacggtc ttcgcatccg agaggacccg ggcgaactcc tcgacggttc
     1261 tcggaccgac gtcgagaccc atgaggcccc ggggaaccgc atccgcgcgg acggtctcct
     1321 tcctcgccga cgcggatacc tcggtcgcgg cgacgacgtc ctccgggagc aggatcagga
     1381 ccccgcgccg ctcggcctcg gccagcgtcg cgcgcacctc ctccacccgg tcgcgctcca
     1441 cgagcgagct cccgatctcg cctccctgcg ccgcgatgaa cgtgaaggcc atcgcgccgc
     1501 cgatcagcag ggcgtcgacg cggtccagga gcgagccgac gacgccgagc ttgtcggaga
     1561 ccttcgcgcc gccgaggatc gcgacgaacg gctcctcggg gtcggtcagg agccgggaga
     1621 ggacctcgac ctcccgctgc atcagcaggc ccgccgccga cgggaggcgc tcggccacgc
     1681 ccacgacgga ggcgtgcgag cggtgggccg ccccgaaggc gtcgtccacg tacgcgtccg
     1741 cgagccgagc gagcgcctcg gcgaaccctg gatcgttcgc ctcctcaccc gggtcgaacc
     1801 gcaggttctc gatgagcgcg acgtccccgg gcccgagcgc tcggcaggcc gcctcgaccg
     1861 ttcctccccg cacctcgtcg agcttcgtca ccgggaaccc gccgaggtcc tggagccgcg
     1921 ccgcgaccgg gtcgagcctt aggtccccgc gcggctggcc cttcgggcgg ccgaggtggc
     1981 tggcgaggac gagcgcggcg ccggcgtcac gcagtcgctc gatcgtcggc aaagccgccg
     2041 cgatccggag gtcgtcggtg accttcccgg cctcgagcgg cacgttgaag tcgacgcgca
     2101 ccagcacgcg ccggccccgg aggtcgccga ggtcgtcgat caggcggagg cggcccacct
     2161 acagggtcga gccgacgagc cgcgcgaggt cgaccaggcg gttcgagtac ccccactcgt
     2221 tgtcgtacca gccgacgacc ttgacgaggt tgcccatcgc catcgtcgac tgcgcgtcga
     2281 agatgcacga gtgcgggtcg ccgacgatgt cgctcgagac gatcgggtcc tcggtgtagc
     2341 ggaggtacgg cgcgagcggc ccgtcgctcg ccgccgtgcg gaacgcttcg ttcacctcgt
     2401 cgaccgtcac gtcccgctcg aggatcgcaa cgaggtcggt gacggaaccg tccggcacgg
     2461 ggacacgcat cgccatgccg tccatccggc ccttcagctc ggggatcgcg agcgacgtcg
     2521 ccttcgccgc gccggtcgag gtcgggatga tgttgacggc ggcggcccgc gcccggcgca
     2581 ggtccttgtg cgggaggtcg aggatcgact ggtcgttcgt gtaggcgtgg atcgtcgtca
     2641 tcagcgcccg gtcgatcccg aagctgtcgc gcaggacctt ggacatgggc acgacggagt
     2701 tcgtggtgca cgacgcgttc gagatcacgt ggtgctcgtt cgggtcgtac tcgccgtcgt
     2761 tcacgcccat cacgagggtg atgtcctcgc ccttcgccgg ggcgctgatg atgaccttct
     2821 tcgcgccggc ggacaggtga agctcagcct tctcgcggtt cgtgaacagc ccggtcgact
     2881 cgatcacgag gtcgacgccg agctccttcc acgggaggtt cccgggatcc cgctcggcga
     2941 ggacgcggaa ctcctcgccg tcgaccgaga tcccctcgtc ggtcgccttg atctccgagt
     3001 cgaggttccc caggacggag tcgtacttga gcaggtgcgc gagcgtcggt gcgtcggtga
     3061 tgtcgttcac cgcgacgagg tcgaagtcgg cgccgcgctc cttggcggcg cggaagaagt
     3121 tgcggccgat gcggccgaag ccattgatcc ctaccttgat cgccatccgg aagcctcctc
     3181 ggtgcgtccc acggtccggt cggttcggtt catcctatcc ggccgaggtc cgggcctccc
     3241 accgctcagg ggcggtcgag gtcccggtgc ttcgcccggg catcgagccc ctgctcgccg
     3301 aaccaccgct cgagcgcctc ggcgacgacg accgagcgat ggcgcccgcc ggtgcagccg
     3361 accgccagcg tgaggtagga cttcccctcg gccacgtagc ccgggacgac gaactcgagg
     3421 agtcctcgca gccgttccat gaattccgcg tacgcgggtt ggtcggtcac gtagccgcgc
     3481 accgcgtcgg ccgtcccggg gagcggccgg agctcgtcga tccagtgggg gttcgggagg
     3541 aaccgcacgt cgaggacgag gtcggcgtcg cgcgggatcc cgtacttgta gccgaaggag
     3601 acgacctgga cctggaggcc ggcgcccggc cggtcccgcg cgaagccgtc gcggatccgc
     3661 tcccggagct ggtgcggggt gagctgggag gtgtcgatga tcaggtctgc ctcgccgcgg
     3721 agcgtctcca tcatcagccg ctccttgcgg atcccctcga ccacgcggtc ccccggcgcg
     3781 agggggtgcc gacgccgggt cgcctcgaag cggttcacga gcgcgtcgtc gttcgcctcg
     3841 aggaacagga tccggtaggg caggcgtcgc tcgtgcaggt cctggagcgc ggcggcgagg
     3901 tccgtgaaga agacgccgcc ccgcgcgtcc gcgacgatcg cgacgcggct cggctcaccg
     3961 gccgtgagca ccagctccgc catcttgccg atcagcgccg gagggaggtt gtcgacgacg
     4021 aagtaaccga ggtcctcgag gtgcttc
//
LOCUS       DATLGG010000287         9521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_216348, whole genome shotgun sequence.
ACCESSION   DATLGG010000287 DATLGG010000000
VERSION     DATLGG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216348"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VM638_08180"
     CDS             complement(<1..673)
                     /locus_tag="VM638_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HVM12436.1"
                     /translation="MTGTLINAAAVLVGTVLGVLIGDRLPERIRETVFHALGLVTLLM
                     GVANGIEAFGPELAQVTRVAPIVVLGSILLGGVLGEALGVERGLNRFGDALKARVGRG
                     QARFTEGFVVATLLFCVGPLAIIGSIQDGLSGNLQILGVKSLLDGFAALAFASALGWG
                     VGFSIIPIVLYQGALSLSAGAVAGIFTEAVVAALTGAGGILIVGIALRLLDLREVRVA
                     NLLPAL"
     gene            complement(670..1386)
                     /locus_tag="VM638_08185"
     CDS             complement(670..1386)
                     /locus_tag="VM638_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HVM12437.1"
                     /translation="MAGIRADCRLSPVAAGAHLTHREYLEAIYEMAEEGIPTLRARLA
                     EWLGITPASVSEGIKRLTARGLVRTGPGRRVEFTPEGEEAARTLVRRHRLAERFLVEI
                     VGLPWHLAHEEASDWGHVVSERVEERMVELLDDPGTCVHGNPIPGSRHAPDQGDLRPL
                     HAIEPPARVRLERLTEDLELDLDVMRFFERSGLMPGRDLRVVSTAPDGTMTLEVEGEK
                     VGLGGHLADNLWVRALEPAG"
     gene            1376..4012
                     /gene="polA"
                     /locus_tag="VM638_08190"
     CDS             1376..4012
                     /gene="polA"
                     /locus_tag="VM638_08190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVM12438.1"
                     /translation="MPATPKRIFLLDGHSLAYRAFFALPTSLATTTGQVTNAAYGFTS
                     MLIKLLAEEKPDHLAVAFDKGRPTIRLAVYAEYKAGRAETPDEFKQQLPLIREVLDTL
                     KVPIVEVEGHEADDAIASLALRAVEEGMEAVIVTADRDFFQLVRPGIRVMFNRKGISD
                     IVWYDEEAVERRFGLPPARYLDYVALKGDPSDNIPGVPGVGEKTASRLIQQYGSVEGV
                     LEHVDDLTPKLAAGIRDAGEDLARNKEVARLRPDITTPLTPGECLMGAWDPAEVRRLF
                     NSLEFRSLLQRLEEIGTTRPVTEVRPLEVAEGTIEDLVRIVGGREPVAFALDAAEGKL
                     HGVAVSAGDGRGVHLRVDGDAPEELRDWLADASRPKVVHDAKELASSAARIGLEVAGV
                     AFDTLLAAYLLDPAEAEYPLPALCERYLGQDLLGAAAEPETEGQLPLEPDPWRRTAGE
                     AAAIALLAPVMREGTDREGLADLLARVELPLAVVLARMQATGITLDVPYLRGMSEEVG
                     DRMRSLEAEIYEHAGEEFNLNSPPQLRVILYERLGLTPGKRTKTGFSTDADTLEKLRH
                     QHPIVDRLLAYRELSKLKSTYLDALPPLVRPDTGRIHTTFNQVAAATGRLSSVNPNLQ
                     NIPVRGDLGRQIRRAFVAGREGWVLLVADYSQIELRVLAHLSGDEALAEAFASDADIH
                     AATAAKVYRLPPDQVDPEMRRFAKVVNYGLAYGMNAYGLASRLGIPPDEAQAFIDAYF
                     EGFPRVREFLERQVGLAAAEGSTSTILGRRRYLPELGSPNPRIRDLGRRMALNAPIQG
                     SAADIIKLAMIRVDRALEGLPAAMLLTVHDELVLEVDAGALERVAEVVRSEMAAAFDL
                     TVPLRADVGWGPTWADAAPAGH"
     gene            4136..5464
                     /gene="rpsA"
                     /locus_tag="VM638_08195"
     CDS             4136..5464
                     /gene="rpsA"
                     /locus_tag="VM638_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006769418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HVM12439.1"
                     /translation="MDDNSGARTAVGEDYTPEELIEAIEASMKEINDGDLVAGKVVRI
                     DRDEVLLDIGYKSEGVIPAKELSIRHDVDPGEEVAIGDELEALVLTKEDKEGRLILSK
                     KRAQYERAWGRIEQTMQEGKTIRGPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLH
                     PFVGQELECKIIELDRNRNNVVLSRRAFLEESQSEGRKKVLESLQKGDRRKGTVSSIV
                     NFGAFVDLGGVDGLVHVSELSWRHVEHPGEVATVGQEVEVEVLDVDLERERVSLSLRA
                     TQEDPWKEFERRYRAGEVIEGEVTKLVPFGAFVRVADGIEGLVHISELSEAHVESAEE
                     IVNVGDRVKVRVVEVDVSRRRVSLSMRRVDEPGMPTGPEIEEEEPMPAAPAPAPAAEA
                     APQAPPEAAREPEEEIVPEAAPAAPAAEEGEDISLEAIVQDLKRREGRTE"
     gene            5490..5957
                     /locus_tag="VM638_08200"
     CDS             5490..5957
                     /locus_tag="VM638_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12440.1"
                     /translation="MYLWWRFVHIAAALAFVMAHGTAVGVAFRIRGERNRERIAALLE
                     LSSRSLVWMYLSLGALVVAGVVLGFMTRAWGRGWIWTAIALLVLLMVLMYALAATFYR
                     RVRDAVTSRRGSGYRISDEELEDILSSGRPHLLALVGFGGLLGILWLMVFRPF"
     gene            complement(5954..6880)
                     /locus_tag="VM638_08205"
     CDS             complement(5954..6880)
                     /locus_tag="VM638_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVM12441.1"
                     /translation="MAYERIVVGTGGGATAERAEARARSLASDLGAEIDAVRADAAAG
                     EDPASAILERAGRRNADLIVVGNRGMHRRVLGSVPDAVSHRAACDVLIVRTHDRPATA
                     PVEPYGAILCATDGTSTADRAARKAHALTRALGADLALLYIGHPATGRSILEDTARQL
                     GDPGIRMVLREGPPAEGIVAAAVEEAADLLVVGTRGLEASRLRSLGSVPDRVAHRAPT
                     DLLLAKTYLLALADLRPGEGAIVDEGGRKLAVAIETDGTQHVLSARCKHLGCTVAWNA
                     GDRTWDCPCHGSRYAADGTVIRGPAQASLDPA"
     gene            6926..7528
                     /gene="coaE"
                     /locus_tag="VM638_08210"
     CDS             6926..7528
                     /gene="coaE"
                     /locus_tag="VM638_08210"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003956644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HVM12442.1"
                     /translation="MLLVGLTGGLASGKSTVAAMLRQRGAVVIDADELARRAVEPGTP
                     GLAAVVAAFGPAVVDAEGRLDREALAAVAFADDEARQRLEAIVHPEVFQLLAAELERH
                     RASGAVVVFEAPLLIETGLDEGCDVVVTVSAAEDRRVERAVSARGIDAEDARARLRAQ
                     LTDAEREARADVVVRNDADVDELERRVEALWQDLRERARG"
     gene            7525..8253
                     /locus_tag="VM638_08215"
     CDS             7525..8253
                     /locus_tag="VM638_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HVM12443.1"
                     /translation="MRIEADIRAVFFDAGETLVYPHPSFPELFAETLRARGHAVDPAF
                     VREVVSVYSQRFSDAAREGSEPTLWSTSREASMRFWLDLYRGFVREVGIDDGVEELAM
                     ALYAAFSDLANYRAHPDAIPTLERLARTDLTLGLISNFEAWLEQLLEAVEITRFFPVR
                     VISGVEGIEKPDPRIFEAALERAGAIAEQSVYVGDHPFFDVEAARAVGMHPVLIDRRG
                     RYPDPGCVRITSLEDLPAAIGVDA"
     gene            8294..8812
                     /locus_tag="VM638_08220"
     CDS             8294..8812
                     /locus_tag="VM638_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVM12444.1"
                     /translation="MERAIGHLDEVLDNLGVFRATAEELVMHCPSGFRRVPISQGPDF
                     YISIQALDAGQVAYAHTHPDSEEWSIVLSGSGEALIHDRIPVASGSIVGRAAAYPHGF
                     RAGADEPLVMLSIQLPRPAEGGTTWDEPGTTTDPIHCRAGGRCRRCARCGGHSAEIVR
                     DRFECENCSQLF"
     gene            complement(8814..9302)
                     /locus_tag="VM638_08225"
     CDS             complement(8814..9302)
                     /locus_tag="VM638_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12445.1"
                     /translation="MRAPRWDALVRPLLVLVVVLCACRPEPQPLDLPRPSGVGTPGLG
                     FFHEGGATIRLGPERFELGLSGAPSTYAAGMLSLSYGELGSGRSLNITGPAREGENRT
                     GPNLSLSIGTDERTYEARDGGCVILVERAGPRGVDGSFRCRRLAGDGVRARASGTFRA
                     SA"
     gene            9326..>9521
                     /locus_tag="VM638_08230"
     CDS             9326..>9521
                     /locus_tag="VM638_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12446.1"
                     /translation="MERSYSVEEANAMLDDLRERIVRLRDARRVLVDGAERVAAGSVA
                     NGGGREGTEYWRALGVVRREG"
BASE COUNT         1328 a         3350 c         3505 g         1338 t
ORIGIN      
        1 ggagggcggg gagcaggttc gcgacccgca cctcgcggag gtcgaggagc cgcagcgcga
       61 tcccgacgat gaggatcccg ccggcccccg tgagtgcggc gaccacggcc tcggtgaaga
      121 tgcccgcgac ggcgcccgcc gagagcgaga gcgctccctg gtagaggacg atcgggatga
      181 tcgagaatcc cacgccccat cccagcgccg aggcgaacgc gagggcggcg aacccgtcga
      241 ggagcgactt cacgcccagg atctgcaggt tgccgctgag cccgtcctgg atcgacccga
      301 tgatcgcgag cggcccgaca cagaacagca gcgtcgccac gacgaacccc tcggtgaacc
      361 tggcctgtcc ccggccgacg cgggccttga gggcgtcccc gaaccggttc agccctcgct
      421 ccacgccgag cgcctccccg agcacgccgc cgaggaggat gcttcccagc accacgatcg
      481 gcgccacccg cgtcacctga gcgagctcgg gcccgaaggc ctcgatcccg ttggccacgc
      541 ccatcaggag ggtgacgagg cccagcgcgt ggaagacggt ttcgcggatc cgctccggca
      601 gccggtcacc gatcaggacg cccaggaccg tcccgaccag gacggccgcg gcgttgatga
      661 gggttccggt catccggcag gctcgagcgc gcggacccag aggttgtcag cgaggtgacc
      721 gccgagaccg accttctcgc cttcgacctc gagggtcatc gtcccgtcgg gagccgtcga
      781 gacgacgcgg aggtcccgcc ccggcatcag cccgctccgc tcgaagaacc gcatgacgtc
      841 gaggtcgagc tcgaggtcct cggtcaggcg ctcgagccgg acgcgcgcgg gaggctcgat
      901 cgcgtggagg ggtcggaggt cgccctggtc gggcgcgtgg cgcgaacccg ggatcgggtt
      961 cccgtggacg cacgtgcccg gatcgtcgag cagctcgacc atgcgctcct cgacacgctc
     1021 ggagacgacg tggccccagt cgctcgcttc ctcgtgggcg aggtgccagg ggaggccgac
     1081 gatctccacg aggaaccgct ccgccagccg gtgccgccgc accagggtcc gcgccgcctc
     1141 ctcgccctcg ggcgtgaact cgacccggcg gccggggccg gtccggacga ggccacgagc
     1201 cgtgagccgc ttgatgccct cggacaccga ggccggggtg atgccgagcc actcggcgag
     1261 cctcgcccgc agcgtcggga tcccttcctc ggccatctcg tagatcgcct cgaggtactc
     1321 gcggtgggtc agatgcgcgc cggcggccac gggcgagagt ctacaatcgg cccggatgcc
     1381 ggccaccccg aagcggatct tcctgctcga cggacacagc ctcgcctacc gcgcgttctt
     1441 cgccctcccg acctccctcg cgacgacgac cggtcaggtg acgaacgccg cctacgggtt
     1501 cacctcgatg ctcatcaagc tgctcgccga ggagaagccc gaccacctcg cggtcgcctt
     1561 cgacaaggga cgtccgacga tccggctcgc cgtgtacgcg gagtacaagg cgggccgtgc
     1621 ggagaccccc gacgagttca agcagcagct cccgctgatc cgtgaggtcc tcgacacgtt
     1681 gaaggtgccg atcgtcgagg tcgaggggca cgaggccgac gacgcgatcg cctcgctcgc
     1741 gctgcgggcg gtcgaggagg ggatggaggc ggtgatcgtc accgccgacc gcgacttctt
     1801 ccagctggtc cgccccggga tccgggtgat gttcaaccgc aagggcatct ccgacatcgt
     1861 ctggtacgac gaggaggcgg tggagcggcg gttcgggctg cccccggctc gctacctcga
     1921 ctacgtcgcg ctgaaggggg acccgtcgga caacatcccg ggggtgccgg gcgtcgggga
     1981 gaagacggcg tcgcggctga tccagcagta cgggtccgtg gagggcgtcc tcgagcacgt
     2041 cgacgatctc acgccgaagc tggcggcggg gatccgcgac gcgggggagg acctcgcgag
     2101 gaacaaggag gtcgcacggc tgcgcccgga catcacgacc cccctcaccc ctggcgagtg
     2161 cctgatgggc gcctgggatc cggccgaggt tcggcggctc ttcaactcgc tcgagttccg
     2221 ctcgcttctc cagcgcctgg aggagatcgg gacgacgcgg ccggtgacgg aggtgcgccc
     2281 cctggaggtg gccgaaggga cgatcgagga cctcgtgcgc atcgtcggcg gacgggagcc
     2341 cgtcgcgttc gccctcgacg cagcggaggg gaagcttcac ggggtcgcgg tctcggcggg
     2401 cgacggccgt ggcgtgcacc tccgggtcga cggcgacgcg cccgaggagc tccgcgactg
     2461 gctcgcggac gcgtcgcggc cgaaggtcgt ccacgacgcg aaggagctgg cttcgtccgc
     2521 ggcgcgcatc ggcctcgagg tcgcgggcgt cgcgttcgac acgctcctcg ccgcctacct
     2581 gctcgatccc gcggaagccg agtacccgtt gcccgcgctc tgcgagcgct acctcgggca
     2641 ggacctgctc ggggcggccg ccgagccgga gaccgaggga cagctcccgc tcgagccgga
     2701 cccgtggcgg cgaacggccg gcgaggcggc tgccatcgcg ctcctcgccc ccgtcatgcg
     2761 tgaggggacc gaccgcgagg gcctcgcgga cctgctcgcg cgcgtcgagc tgccgctcgc
     2821 ggtcgtcctc gcgcggatgc aggcgaccgg catcacgctc gacgtgccgt acctgcgcgg
     2881 gatgtcggag gaggtgggcg accggatgcg ctccctcgag gccgagatct acgagcacgc
     2941 cggggaggag ttcaacctca actcgccgcc gcagctccgc gtgatcctct acgagcgcct
     3001 cgggctgacg ccggggaagc gcaccaagac cgggttctcg accgacgccg acacgctcga
     3061 gaagctccgg caccagcacc cgatcgtcga ccgcctgctc gcgtaccggg agctctcgaa
     3121 gctgaagtcg acctacctcg acgcgctccc gccgctcgtg cgccccgata cgggccgcat
     3181 ccacacgacg ttcaaccagg tcgcggcggc gacgggccgg ctttcctcgg tgaacccgaa
     3241 cctgcagaac atccccgtcc gcggcgacct cggccggcag atccgccggg ccttcgttgc
     3301 gggacgcgag gggtgggtcc tgctggtcgc cgactactcg cagatcgagc tgcgggtcct
     3361 cgcgcatctc tcgggggacg aggcgctggc ggaagccttc gcctccgatg cggacatcca
     3421 cgccgcgacg gcggcgaagg tctaccgcct gccgcccgac caggtcgacc cggagatgcg
     3481 gcgcttcgcg aaggtcgtga actacggcct cgcctacggc atgaacgcct acggcctcgc
     3541 ttcccgactc gggatcccac cggacgaggc gcaggcgttc atcgacgcgt acttcgaggg
     3601 cttcccgcgc gtccgggagt tcctcgagcg gcaggtgggg ctcgcggcgg cggaggggtc
     3661 gacgtcgacg atcctcggtc gccggcgcta cctccccgag ctcggctcgc cgaacccgcg
     3721 gatccgcgac ctcggccgcc ggatggcgct gaacgccccg atccagggct cggccgccga
     3781 catcatcaag ctcgcgatga tccgggtcga tcgggccctc gaagggctgc cggccgcgat
     3841 gctgctgacc gtccacgacg agctggtgct cgaggtcgat gcgggcgcgc tcgagcgcgt
     3901 ggccgaggtg gtgcgttcgg agatggccgc cgccttcgac ctgacggtcc cgctgcgcgc
     3961 ggacgtcgga tgggggccca cctgggccga tgccgcgccg gccggccact gagccgattg
     4021 acgcctctgc tccggctccc ctaagattgg cgggctcgcg gccctccgcg cgacccccct
     4081 ctcatccggg tggctgtcgc tggatggccc gctcgagcgg aaggagctga tcgggatcga
     4141 cgacaacagc ggagcgcgca cggccgtcgg ggaggattac acccccgagg agctgatcga
     4201 ggcgatcgaa gcctcgatga aggagatcaa cgacggcgac ctcgtcgccg gcaaggtcgt
     4261 gcgcatcgac cgcgacgagg tgctcctcga catcggctac aagtccgagg gcgtcatccc
     4321 cgcgaaggag ctgtcgatcc gccacgacgt cgatccgggc gaagaggtcg cgatcggcga
     4381 cgagctcgag gcgctcgtcc tcacgaagga ggacaaggaa ggccgcctga tcctgtcgaa
     4441 gaagcgggcg cagtacgagc gcgcgtgggg ccgcatcgag cagacgatgc aggagggcaa
     4501 gacgatccgc ggcccggtga tcgaggtcgt gaagggcggg ctgatcctcg acatcggtct
     4561 ccggggcttc ctcccggcgt cgctcgtcga ccttcgtcgc gtgcgggacc tgcacccctt
     4621 cgtcgggcag gagctcgagt gcaagatcat cgagctcgac cgcaaccgca acaacgtggt
     4681 cctctcgcgc cgcgccttcc tcgaggagtc gcagagcgag ggtcgcaaga aggtcctcga
     4741 gtcgctccag aagggcgacc gtcggaaggg caccgtctcg tcgatcgtca acttcggggc
     4801 gttcgtggac ctcgggggcg tcgacgggct cgtgcacgtc tccgagctgt cgtggcggca
     4861 cgtcgagcat cccggcgagg tcgcgacggt cggccaggaa gtggaggtcg aggtgctcga
     4921 cgtcgacctc gagcgcgagc gggtctcgct gtcgctgcgg gcgacccagg aggatccgtg
     4981 gaaggagttc gagcgccgct accgcgcggg tgaggtgatc gagggcgagg tgacgaagct
     5041 cgtcccgttc ggcgccttcg tgcgcgtggc ggacggcatc gaggggctgg tccacatcag
     5101 cgagctctcc gaggcgcacg tggagtccgc cgaggagatc gtgaacgtcg gcgaccgggt
     5161 caaggtgcgg gtcgtggagg tggacgtctc ccgtcgccgc gtctccctct cgatgcgccg
     5221 ggtcgacgag ccggggatgc cgaccgggcc cgagatcgaa gaggaggagc cgatgccggc
     5281 cgcgcccgct ccggcccccg cggcggaggc tgcgccgcaa gcccctcccg aggcggcacg
     5341 cgagcccgag gaggagatcg tccccgaggc cgcgcccgcc gctccggccg ccgaggaagg
     5401 cgaggacatc tcgctcgagg cgatcgtgca ggacctgaag cgccgcgagg gccgcaccga
     5461 gtagcccctc ccgcgggagg atcgatccga tgtacctgtg gtggaggttc gtccacatcg
     5521 ccgccgcgct cgcgttcgtc atggcgcacg gcaccgcggt cggcgtcgcc ttccggatcc
     5581 gcggcgagcg caaccgggag cggatcgcgg cgctcctcga gctgtcctcg cggtcgctcg
     5641 tgtggatgta cctctcgctc ggagcgctcg tcgtcgccgg agtcgtcctc gggttcatga
     5701 cccgcgcctg gggacgggga tggatctgga ccgcgatcgc gctcctcgtg ctgctgatgg
     5761 tgctgatgta cgcgctcgcg gcgacgttct accggcgcgt ccgagacgcc gtcacctcgc
     5821 gccgagggag cgggtaccgg atcagcgacg aggagctcga ggatatcctg tcctcgggac
     5881 ggccccacct gctggcgctc gtcggcttcg gcgggctgct ggggatcctc tggttgatgg
     5941 tcttccgccc gttctaggcg ggatcgaggg acgcctgcgc cggtccccgg atgaccgtgc
     6001 cgtccgccgc gtagcgcgag ccgtgacagg ggcagtccca ggtccggtcg ccggcgttcc
     6061 acgcgaccgt gcatccgagg tgcttgcagc gagcggacag gacgtgctgg gtcccgtcgg
     6121 tctcgatcgc gacggcgagc ttccgaccgc cctcgtccac gatcgcgccc tctcccgggc
     6181 ggaggtcggc gagcgcgagg aggtacgtct tggccagcag gaggtccgtg ggcgcgcggt
     6241 gcgcgacgcg gtcggggacc gacccgagcg agcgcagccg ggaggcttcg agaccccgcg
     6301 tgccgacgac gagcaggtcg gcggcctcct cgacggccgc cgcgacgatg ccctcggccg
     6361 ggggcccctc gcgcaggacc atgcggatgc cgggatcgcc cagctggcgg gccgtgtcct
     6421 ccaggatgct gcgtccggtg gcggggtgac cgatgtagag cagcgccaga tcggcgccga
     6481 gcgcccgggt gagcgcgtgg gccttgcgtg cggcccggtc cgcggtcgag gtgccgtcgg
     6541 tcgcgcacag gatcgccccg tatggttcca ccggtgccgt cgcggggcgg tcgtgggtgc
     6601 ggacgatgag gacgtcgcag gcggctcggt gcgagaccgc gtcgggaacg cttccgagga
     6661 cgcggcggtg catccccctg ttcccgacca cgatcaggtc ggcgttccgc cggcccgccc
     6721 tctcgaggat cgccgaggcg ggatcttcgc ccgcggcggc gtctgccctg accgcgtcga
     6781 tctccgcgcc gaggtcggag gcgagggagc gcgcccgcgc ctccgcgcgc tccgcggtcg
     6841 cccctccgcc ggtgccgacg acgatccgct cgtaggccat cgcgcggagc ctatcgcgac
     6901 gacccgccgc ggctatcctt cgctgatgct cctcgtcggt ctcacgggcg ggctcgcctc
     6961 ggggaagtcc accgtggcgg cgatgctccg ccaacggggg gcggtcgtga tcgatgccga
     7021 tgagctcgca cggcgtgcgg tcgagccggg cacgccgggg ctcgccgccg tggtcgcggc
     7081 gttcggcccc gcggtcgtgg acgccgaggg gcggctcgat cgcgaggcgc tggcggcggt
     7141 cgccttcgcc gacgacgagg cccgtcagcg gctcgaggcg atcgtccatc cggaggtgtt
     7201 ccagctcctt gccgcggagc tcgagcggca ccgcgcgagc ggcgcggtcg tcgtgttcga
     7261 ggcgccgctg ttgatcgaga ccggtctcga cgagggatgc gacgtcgtcg tgacggtctc
     7321 cgcggcggag gatcggcgcg tggagcgagc cgtctcggca cgcgggatcg acgcggagga
     7381 tgcccgcgcg cggttgcgcg cgcagctgac cgatgcggag cgggaagcgc gagccgacgt
     7441 cgtcgtccgg aacgacgccg acgtggacga gctcgagcgt cgcgtcgagg ctctgtggca
     7501 agacctgcgg gagcgagcgc gaggatgagg atcgaggccg acatccgtgc cgtgttcttc
     7561 gacgcgggcg agacgctcgt ctacccccat ccgtcgttcc cggagctctt cgccgagacg
     7621 ctccgagccc gaggccacgc cgtcgacccg gcgttcgttc gcgaggtcgt ctccgtctac
     7681 tcccagcggt tctccgacgc cgcccgggag gggagcgagc cgaccctctg gtcgacgtcg
     7741 cgcgaggcct cgatgcgttt ctggctggac ctctaccggg ggttcgtccg cgaggtgggg
     7801 atcgacgacg gggtcgaaga gcttgcgatg gccctctacg cggccttctc cgacctcgcg
     7861 aactaccgtg cgcatcccga tgcgatcccg acgctcgagc ggctggcgcg caccgacctc
     7921 acgctcggcc tcatctcgaa cttcgaggca tggctcgagc agctcctcga ggccgtcgag
     7981 atcacgcggt tcttccccgt gcgcgtgatc tcgggggtcg aggggatcga gaagcccgac
     8041 ccccgcatct tcgaggccgc gctcgagcgc gcgggcgcga tcgcggagca gtcggtctac
     8101 gtgggggacc acccgttctt cgacgtcgag gccgcacgcg ccgtcgggat gcacccggtg
     8161 ctgatcgatc gtcgggggcg ctaccccgac cccgggtgcg tccgcatcac ctccctcgag
     8221 gacctccccg ccgcgatcgg ggtcgacgcc tgaggacttg cggctcggtc cgccggcggc
     8281 tttgatgacg ccgatggagc gagcgatcgg ccacctcgac gaggtcctcg acaacctcgg
     8341 ggtcttccgc gcgacggcgg aggagctggt gatgcactgt ccgtccgggt tccgacgcgt
     8401 cccgatcagc cagggccccg acttctacat ctcgatccag gcgctcgacg cgggacaggt
     8461 ggcgtacgcc cacacgcatc ccgactccga ggagtggtcg atcgtgctga gcgggtccgg
     8521 cgaggcgctg atccacgacc ggatccctgt cgcctccggc tcgatcgtcg gccgggctgc
     8581 agcctatccc cacgggttcc gcgccggcgc ggatgagccg ctcgtcatgc tctcgatcca
     8641 gctcccgcgt ccggcggaag gcggaacgac gtgggatgag ccggggacga cgaccgatcc
     8701 gatccactgc cgggccggcg gccggtgtcg gcgctgcgcg cgctgcgggg ggcacagcgc
     8761 cgagatcgtc cgcgaccggt tcgagtgcga gaactgctcg cagctgttct gagctacgcc
     8821 gacgcccgga acgtgcccga ggcccgcgcg cggaccccgt cgcccgcgag gcggcggcac
     8881 cggaacgatc cgtcgacgcc gcgtgggccg gcgcgctcca cgaggatgac gcagccgccg
     8941 tcccgggcct catacgtccg ttcgtcggtc ccgatggaca ggctcaggtt cgggccggtg
     9001 cggttctcgc cctcacgcgc cggccccgtg atgttgaggg accgtccgct gccgagctcg
     9061 ccgtaggaga ggctgagcat ccccgccgcg tacgtcgacg gcgcgcccga cagaccgagc
     9121 tcgaaccgtt cggggccgag gcggatcgtc gcgcccccct cgtggaagaa cccgagaccg
     9181 ggcgtcccga caccggaggg acggggaagg tcgagcggct gcggttccgg gcgacacgcg
     9241 cacaggacga ccacgaggac gaggagcggg cgcacgagcg catcccaccg cggcgcgcgc
     9301 acccccgtca acctatgctg gtccggtgga gcgctcctac tcggttgagg aagcgaacgc
     9361 gatgctcgac gacctccggg agcggatcgt ccgcctgcgt gacgcgcggc gcgtcctcgt
     9421 cgacggagcc gagcgtgtgg cggcgggatc cgtggcgaac ggtggcggcc gcgaggggac
     9481 cgagtactgg cgggcgctcg gcgtggtgcg gcgggagggc g
//
LOCUS       DATLGG010000288         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_201374, whole genome shotgun sequence.
ACCESSION   DATLGG010000288 DATLGG010000000
VERSION     DATLGG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201374"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VM638_08235"
     CDS             <1..186
                     /locus_tag="VM638_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12447.1"
                     /translation="AGVGVTLVLPEQRGDVSAIARQLKLNDAFEAEGMKISPPRLVFA
                     SRRGSRSQLAGRRGRRF"
     gene            complement(183..1694)
                     /locus_tag="VM638_08240"
     CDS             complement(183..1694)
                     /locus_tag="VM638_08240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12448.1"
                     /translation="MRILVWHGWLLGGAGSNVYTARTVEIWRRQGHDVLLLCQERRVE
                     KQGFFDAHGASDDLDRLEPLTGEPAAGRAILIRPAIGDLLPVFVHDAYEGFRVKRFVD
                     LSDEELARYLKANVAAVRAAAEWHRSEVAVVGHAFPGGVVGARALQGIPYVVKVHGSD
                     IEYAIRSQERYRELTREGLHRAAAVAGASRDVLERLAELVPHPGGRARVVPPGVESGR
                     FRPAERRASLRWAAEDLATAGDDGRTPAIDEEVATALRRRDLPALDALANRYDQAAPD
                     AAAPRKLRALADHEGPLVGYLGKLIPQKGVEHLIAALALVPWARGVIVGYGTFREWLE
                     ALATALDHADEDLVRLLGDRMPLSLTPTEVTGARGLQDRLTFTGRLDHDHAPAVVAAC
                     DVLAVPSVLDEAFGMVAIEGAAAGALPLVARHSGLAEIAAALEEEAGRPGLFSYAPGP
                     DPVRNLVAGLGRLFGVPADERRELALGLAAFAATRWGWQRTADALLASVRGGS"
     gene            complement(1765..>2844)
                     /locus_tag="VM638_08245"
     CDS             complement(1765..>2844)
                     /locus_tag="VM638_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12449.1"
                     /translation="VYVSSYPSGARVTRREDLATFPDQPDCLPDHNRISIVEIPDRNP
                     AAAHLLKEQPLDQDTAVWTATGSGGSPVRGCHDITFFEFSDRRKVAAGACLSEGQLWD
                     VSDPANPTTTVPGRHSHIRNPFIDGPNPPQRGLFHTASFSWDGEVVLFTDEWAGGGGH
                     GCDGPQDTRGNVWFYGNTPPGTQPPLYGRFITPRPQPANEICTMHNGNVIPTNEGYYG
                     VSSAYQAGTSVFDFSGVTELDPIPQPLTGTEAIPTVATEVAYFDAKNDPSGPQGIDDA
                     WSTYWHNDYVYASSGLTNASPSRPGARGFDVYLLLGRNGRLAEEGSPTVTGDDLEDPA
                     VKHYRARKFRYTNPQTQEVNQNLGR"
BASE COUNT          367 a         1037 c         1030 g          410 t
ORIGIN      
        1 gccggtgtcg gcgtgacgct cgtcctcccg gagcagcgcg gtgacgtgag cgcgatcgcg
       61 cgtcagctga agctcaacga cgcgttcgag gccgagggga tgaagatctc cccgccgcgg
      121 ctcgtgttcg catcgaggcg cgggagccgc tcgcagctcg ccggccgacg cgggcgccgc
      181 ttctagctcc cgccacgcac cgacgccagc agggcgtcgg ccgtccgctg ccacccccaa
      241 cgggtggcgg cgaacgcggc cagcccgagc gccagctctc gccgttcatc tgcgggaaca
      301 ccgaagagac gtccgagccc ggcgacgagg ttccgcacgg ggtcagggcc cggtgcgtag
      361 gagaacagtc ccggccggcc ggcctcttct tcgagcgcgg cggcgatctc ggcgaggccg
      421 ctgtggcggg cgacgagggg gagcgcgccc gccgcggccc cttcgatcgc gaccatcccg
      481 aacgcttcgt cgagcaccga cggaacggcg agcacgtcgc aggctgccac gacggccggc
      541 gcatggtcgt gatcgaggcg cccggtgaac gtgagccggt cctgcagccc acgggcaccg
      601 gtgacctccg tcggggtgag ggacaggggc atccgatcgc cgaggagccg aacgaggtcc
      661 tcgtccgcgt ggtccagcgc cgtggcgagc gcctcgagcc actcgcggaa cgtgccgtag
      721 ccgacgatca ccccgcgcgc ccaaggcacg agcgccagcg ccgcgatcag gtgttcgacc
      781 cccttctgcg ggatcagctt cccgaggtag ccgacgaggg gcccctcgtg atcggcgagc
      841 gcccggagct ttcgcggcgc ggcggcgtcg ggagccgcct ggtcgtagcg gttcgccagt
      901 gcgtcgagcg ccggaaggtc gcgtcgtcga agcgcggtcg ccacctcttc gtcgatcgcg
      961 ggcgtccggc cgtcgtcgcc cgccgtcgcc aggtcctccg cggcccagcg cagcgacgct
     1021 cggcgctcgg ccggccggaa ccggccgctc tccacgcccg gcgggaccac ccgcgcgcgc
     1081 cccccggggt gcgggacgag ctcagcgagc cgctcgagga cgtcccggga ggcgccggcc
     1141 acggccgccg cgcggtggag cccctcccgc gtcagctccc ggtaccgctc ctgggatcgg
     1201 atcgcgtact cgatgtcgct gccgtgaacc ttgacgacgt aggggatgcc ctggagcgcg
     1261 cgggccccca cgaccccccc ggggaaggca tgtccgacga cggcgacctc ggaccggtgc
     1321 cactcggcgg ccgcgcggac cgccgcgacg ttcgccttga ggtaccgggc gagctcctca
     1381 tccgagaggt cgacgaaccg cttgacccgg aacccctcgt aggcgtcatg gacgaagacc
     1441 ggcagcaggt cgccgatcgc cggccggatc aggatcgcgc ggcccgccgc gggctcgccg
     1501 gtgagcggtt cgagccggtc gaggtcgtcg ctcgcgccgt gcgcgtcgaa gaaaccttgc
     1561 ttttcgaccc ggcgctcctg gcagagcagc aggacgtcgt gcccctgtcg ccgccagatc
     1621 tcgacggttc gggcggtgta gacgttcgag ccggcgcctc cgaggagcca cccgtgccag
     1681 acgaggatcc tcacccgccg agcctaccga tgacgggaga cgggggcgcc gtcgccggcg
     1741 cccccgtcga tgcctctcgc cccgctaccg gccgaggttc tggttgacct cctgggtctg
     1801 cgggttcgtg tagcggaact tcctcgcgcg gtagtgcttg accgccgggt cctcgaggtc
     1861 gtcccccgtc acggtggggc tcccctcctc ggcgagccgt ccgttccgcc cgaggagcag
     1921 gtacacgtcg aagcccctgg cgcccggccg cgaaggtgag gcgttcgtca gcccgctcga
     1981 ggcgtacacg tagtcgttgt gccagtaggt cgaccacgcg tcatcgatgc cctgcgggcc
     2041 gctcgggtcg ttcttcgcgt cgaagtacgc cacttcggtc gcgaccgtgg ggatggcctc
     2101 ggtgcccgtg agcggctgcg ggatcgggtc gagctcggtc accccgctga agtcgaagac
     2161 cgacgtcccg gcctggtacg cgctcgagac gccgtagtag ccctcgttcg tcgggatgac
     2221 gttcccgttg tgcatcgtgc agatctcgtt cgccggctgc ggccgaggcg tgatgaaccg
     2281 gccgtacagc ggcggctgcg tccccggcgg cgtgttgccg tagaaccaga cgttcccccg
     2341 ggtgtcctgc gggccgtcgc acccgtggcc gccgccgccc gcccactcgt cggtgaagag
     2401 cacgacctcg ccgtcccagc tgaaggaggc cgtgtggaag aggccgcgct gcggcgggtt
     2461 cggcccgtcg atgaacgggt tgcggatgtg gctgtgccgc cccgggaccg tcgtcgtcgg
     2521 gttagccgga tcggacacgt cccagagctg cccctcgctc aggcaggccc cggccgcgac
     2581 cttccgccgg tccgagaact cgaagaacgt gatgtcgtgg cagccgcgga ccgggctgcc
     2641 accgctgccc gtcgccgtcc agaccgcggt gtcctggtcg agcggctgct ccttcaacag
     2701 gtgcgccgcc gccgggttgc ggtccgggat ctcgacgatc gagatgcggt tgtggtccgg
     2761 caggcagtcc ggctggtccg ggaacgttgc caggtcctca cgccgcgtca cccgtgctcc
     2821 gctcgggtac gaggagacgt acac
//
LOCUS       DATLGG010000289         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_154691, whole genome shotgun sequence.
ACCESSION   DATLGG010000289 DATLGG010000000
VERSION     DATLGG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154691"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VM638_08250"
     CDS             complement(<1..407)
                     /locus_tag="VM638_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVM12450.1"
                     /translation="MPLIAQRPPITSREWLLSNGVRILVILVVAFLVSWIARQAVRRM
                     QRRLEGADSVTQAIDIQRATTLTQSLSYLIRLVIWSLAILMVLDQFEVNLAPLIAGAG
                     IAGVALGFGAQSLVRDFLSGFFILLENQFGIGDV"
     gene            complement(407..2071)
                     /locus_tag="VM638_08255"
     CDS             complement(407..2071)
                     /locus_tag="VM638_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVM12451.1"
                     /translation="MRRALPGVAVLALLALATPQAGAGDRRVRVEAEPSATLLVHGEY
                     PERTSNCVNPSQPLLHARYRGTIEVGRDAHGSLFVIGELPFEDYLKGIAEVPRRWHLE
                     ALKAQVVAARSYAMSRLRLGSSEARRLGYDLCATEACQVYLGMGIEAGPWGERWVRAV
                     EGTAGEVLLYEGRPADALYSSTSPGRTFDNEDVFGGSPLPYLRGGPERDDGESPLAHW
                     EVRFALDDLARFLRAAGAWGGERIQGVTIRSGRIALRGGGRRLRLTRAELRSALNDVA
                     SCLDERYPSREPDGYRLPQTVPSVWFEAEREGDDLVLRGRGWGHGVGMVQWGAKGKAD
                     RGLGYGDILAAYYGGLRPQRVETPETIRVLVADGLRRVTVVPHGPATIAGLGRDVDPP
                     WVLTARRRGVRVRRGPQPPPALAVEARRAALREGRLTLPIDLPKDARVRLELVDEDGR
                     VTTTPWRPRVRGRHRLDETPPAGPGSYRVRVVADDGVDRIRRGAGTVVLAGAARPTED
                     PAPPPGAPGEPGRALPVPAVLIPLVAAVLGLGAWLTLRRRRGFHRG"
     gene            complement(2068..>2504)
                     /locus_tag="VM638_08260"
     CDS             complement(2068..>2504)
                     /locus_tag="VM638_08260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVM12452.1"
                     /translation="PGDPGSPVALPEDPLALPEQDPETFRRLIASLRPTPVVVNIWGS
                     WCPPCREEAPVLARISEKYEGRVRFIGVDILDEREAAREFIREYGWTYPSVFDPQARI
                     RDEMGYVGQPITVIHDREGEIAFEWNGAISAERLEAEIQKVL"
BASE COUNT          361 a          923 c          914 g          306 t
ORIGIN      
        1 acgtcgccga tgccgaactg gttctcgagc aggatgaaga accccgacag gaagtcccgg
       61 acgaggctct gcgccccgaa cccgagggcg acgccggcga tccccgcgcc cgcgatgagg
      121 ggcgcgaggt tcacctcgaa ctggtcgagc accatgagga tcgcgagcga ccagatcacg
      181 agacggatca ggtacgagag ggactgcgtg agggtggtcg cccgctggat gtcgatcgcc
      241 tgcgtcacgc tgtcggcgcc ctcgaggcgc cgctgcatcc ggcggaccgc ctgtcgggcg
      301 atccacgaaa ccaggaaggc gacgacgagg atcacgagga tccggacgcc gttggacaat
      361 agccactcgc ggctggtgat cggggggcgc tgggcgatga ggggcatcac ccgcgatgaa
      421 accccctccg gcgacggagg gtcaaccacg ccccgagccc gaggacggcc gcgaccagcg
      481 ggatgagcac ggccggtacc ggcagcgccc gaccgggttc cccgggcgcc cccggaggcg
      541 gcgccgggtc ctccgtcggc ctcgctgccc cggcgagcac gacggttccg gcaccgcggc
      601 ggatccgatc gaccccgtcg tccgcgacca cccgcacccg gtacgacccg ggcccggcgg
      661 gcggcgtctc gtcgagccgg tgccggccac ggacgcgcgg ccgccagggc gtcgtggtca
      721 cacggccgtc ctcgtcgacg agctcgagcc ggacccgcgc gtccttggga aggtcgatcg
      781 gcagggtgag acgtccctcg cggagcgccg cacgacgcgc ctcgaccgcg agcgccggcg
      841 gcggctgtgg gcctcgtcga acgcggacgc cgcgcctgcg agcggtgagg acccacgggg
      901 ggtcgacgtc gcgtccgagg ccggcgatcg tcgcgggccc gtgcgggacc acggtcacgc
      961 ggcggagccc gtcggcgacg aggacccgga tcgtctccgg cgtctcgacc cgctgcgggc
     1021 ggagcccccc gtagtaagcg gcgaggatgt ccccgtaccc cagaccgcgg tcggccttgc
     1081 ccttcgcgcc ccactgcacc atcccgacgc cgtgccccca tcctcgtcct cgaagcacga
     1141 ggtcatcccc ttcgcgctcc gcctcgaacc agaccgacgg cacggtctgg gggagccggt
     1201 atccatcggg ctcgcggctc gggtaccgct cgtcgaggca ggaggcgacg tcgttgagcg
     1261 cggaccgcag ctccgcccgg gtgaggcgca ggcgtcgtcc acctccgcgc agcgcgatcc
     1321 gcccggagcg gatcgtcacg ccctggatcc gctcgccgcc ccaggctccg gcggcgcgga
     1381 ggaaccgagc caggtcgtcg agcgcgaagc gaacctccca gtgggcgagc ggtgactcac
     1441 cgtcgtcgcg ttccggaccc ccgcgcaggt aggggagggg ggagccgccg aacacgtcct
     1501 cgttgtcgaa ggtgcgcccg ggcgaggtcg aggagtagag cgcgtccgca ggccgcccct
     1561 cgtagagcag aacctcaccc gcggtccctt ccaccgcgcg gacccagcgc tccccccagg
     1621 gcccggcctc gatccccatc ccgaggtaga cctggcaggc ctcggtcgcg cagaggtcgt
     1681 agccgagacg tcgcgcctcc gacgagccga ggcgcagccg gctcatcgcg taggagcgtg
     1741 ccgccaccac ctgcgccttc agggcctcga ggtgccagcg cctcggcacc tcggcgatcc
     1801 ccttcaggta gtcctcgaac ggcagctcgc cgatcacgaa cagcgagccg tgggcgtccc
     1861 gcccgacctc gatcgtcccg cggtaccgcg cgtgcaggag cggctgggag gggttcacgc
     1921 agttcgaggt tcgctcgggg tactcgccgt ggacgaggag ggtggcggag ggctccgcct
     1981 cgacccggac gcggcggtcg ccggcgcccg cctggggcgt cgcgagggcg aggagcgcca
     2041 ggaccgcgac gccggggagc gcccgcctca caggaccttc tggatctccg cctccagccg
     2101 ttccgccgag atggcgccgt tccactcgaa cgcgatctcg ccctcgcggt cgtggatcac
     2161 ggtgatcggc tgaccgacgt agcccatctc gtcgcggatc cgcgcctgcg ggtcgaagac
     2221 gctcgggtag gtccagccgt actcacggat gaactcgcgc gccgcctcgc gctcgtcgag
     2281 gatgtcgacg ccgatgaacc ggacccgtcc ctcgtacttc tccgatatcc gcgcgaggac
     2341 gggcgcctcc tcccggcagg gcgggcacca cgacccccag atgttgacga cgaccggggt
     2401 cggccgcagc gaggcgatca gccgacggaa ggtctcgggg tcctgttcgg ggagggcgag
     2461 cggatcctcg ggcagggcga cgggggagcc gggatccccg gggg
//
LOCUS       DATLGG010000290         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_105787, whole genome shotgun sequence.
ACCESSION   DATLGG010000290 DATLGG010000000
VERSION     DATLGG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105787"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VM638_08265"
     CDS             <1..1459
                     /locus_tag="VM638_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVM12453.1"
                     /translation="GNRYVPGDSSLAPLPPQVIFEGDSLRHTNQDQDLHDLMALDNGP
                     DGKPWFQSKLIGPGETDMVPVEGLKPGVYSYTCSIHPFMGGRLEVRKRLEPQEPAPDH
                     RVDSGENYFAPKTITVVAGSSIEWTNVGRVGHSVTASDGSWDSHPTCPTPGPCHMPGE
                     RYRRTFTEPGTIRYYCKLHGTPGGTGHAGTIVVVPRGDPPSSVDSLFASGGPSVVSVQ
                     GSASFGGELPVAVASDPSGDAPAAAAADRTGLDLTGASVYRADPSLPHLTFEWRLAAL
                     PDAGIPEAVRYSLPFRAGSQRFVVQAKRTNLTGTTIADDPAGHASAQPRFQLLGDCTE
                     STPASCRHIAWLDGHVDLAAKAIRAKVPFGIRPEIAPGATLERATGSVARLTRIEAAL
                     EAASTTIAADDAEWGGANASFAYTIASPVVRLGIAPTGTPEEQVVFSTTASVGTDGGF
                     TGGIATGDLPRGTYDVWARACFGTNCGATKRTLEI"
     gene            1471..2415
                     /locus_tag="VM638_08270"
     CDS             1471..2415
                     /locus_tag="VM638_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12454.1"
                     /translation="MRKLITAAAAAGMLATVLPASAHNINPPSSRVDEVYADVSEALI
                     EVSGRAFFGGQRLLTVGTDPAEDFAPGPLDLTAAQIGQLDANTGELSFVLKVRDLPPN
                     VAQVSGIPEAGRYMWDFSIDMGGADPVLFAIDGRLTNVARGQSTRIPAFELQSNCTRD
                     GNLITCEKVADLTTEMIGSTDEIFVDIPLSVLQQHAGGSVAGRTIGEASICQGISAHL
                     SPGLAFCGGTGGPLTSDTLTLSETSGTYTIASKRVRATIAPAGGGPSQTVEATVSPDG
                     SFLANISRTGLAPGTYTVTAQACYGTNCATKSTTATIS"
     gene            complement(2422..>2732)
                     /locus_tag="VM638_08275"
     CDS             complement(2422..>2732)
                     /locus_tag="VM638_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVM12455.1"
                     /translation="EKGRLIKVGVNEFVDEDETPLDTLKIPHAVEVEQIEGVQRLRGE
                     RDDANARNALERLKAAAADESVNMMPALIDCARAYCTEGEIVTALKEIFGEYTETPRF
                     "
BASE COUNT          413 a         1013 c          915 g          391 t
ORIGIN      
        1 cgggaaccgt tacgtccccg gggactcgag cctcgcgccg ctgcccccgc aggtcatctt
       61 cgagggcgac agcctccggc atacgaacca ggaccaggat ctccacgacc tgatggccct
      121 cgacaacggg ccggatggca agccgtggtt ccagagcaag ctgatcggac ccggcgagac
      181 cgacatggtc cccgtcgaag gcctgaagcc cggcgtctac tcctatacgt gcagcatcca
      241 ccccttcatg ggcgggcgcc tcgaggtgcg gaagcgcctc gagccgcagg agcccgcgcc
      301 cgaccatcgc gtcgactcgg gcgagaacta cttcgctccg aagaccatca cggtcgtcgc
      361 cggctcgtcg atcgagtgga cgaacgtcgg ccgcgtcggt catagcgtga ccgcgagcga
      421 cggcagctgg gactcgcacc ccacctgccc cacccccggt ccgtgccaca tgcccggcga
      481 gcggtaccgc cgaacgttca cggaaccggg aacgatccgc tactactgca agctccacgg
      541 aacgcccggc gggacgggtc acgccggcac gatcgtcgtg gttccgcgcg gcgatccgcc
      601 ctcgtcggtg gattcgctgt tcgcatccgg cggtccgagc gtcgtctcgg tccagggcag
      661 cgcgagcttc ggcggtgagc tgccggttgc ggttgcgtcc gatccttcgg gtgacgcccc
      721 cgcggcggcg gccgccgacc ggacggggct cgacctcacc ggcgccagcg tctaccgggc
      781 cgacccctcc ctgccccacc tgaccttcga gtggaggctc gccgcgctcc ccgacgcggg
      841 catccccgag gccgtgcgct actcgctccc gttccgagcc ggcagccagc ggttcgtggt
      901 tcaggcgaag cgcacgaacc tgaccgggac gacgatcgcc gacgacccgg ccgggcacgc
      961 gagcgcgcag ccgcgcttcc agctcctggg cgactgcacc gagtcgacgc cggcgagctg
     1021 tcggcacatc gcctggctcg acggacatgt cgacctcgcc gccaaggcga tccgggccaa
     1081 ggtcccgttc gggatccggc ccgagatcgc gcccggtgcc accctggagc gcgccaccgg
     1141 ctcggtcgcg cggctgacca ggatcgaagc cgcgctcgag gcggcatcga ccaccatcgc
     1201 ggcggacgac gcggaatggg gcggggcgaa cgcctcgttc gcgtacacga tcgcctcccc
     1261 cgtcgtccgg ctcgggatcg cgccgaccgg gacgccggag gaacaggtcg tcttctcgac
     1321 gaccgcctcc gtcggcaccg acggtggctt caccgggggg atcgccacgg gcgatcttcc
     1381 ccggggcacc tacgacgtct gggctcgcgc gtgcttcggc acgaactgcg gcgccacgaa
     1441 acggacactg gagatctgag aggagaagcc atgcgcaagc tcatcacggc cgcggctgcg
     1501 gcggggatgc tggcgacggt cctgccggcc tccgcgcaca acatcaaccc cccctcgtca
     1561 cgggtcgacg aggtctacgc ggacgtcagc gaggcgctca tcgaggtgag cggccgcgcg
     1621 ttcttcggcg gccagcgtct cctgacggtc gggaccgacc cggccgagga cttcgcgccc
     1681 ggaccgctcg acctcacggc ggcgcagatc ggccagctcg atgcgaacac gggcgagctg
     1741 tccttcgtcc tcaaggtccg cgacctcccc ccgaacgtcg cacaggtcag cgggatcccc
     1801 gaggcggggc gctacatgtg ggacttctcg atcgacatgg gcggcgcgga cccggtgctg
     1861 ttcgcgatcg acggccggct cacgaacgtc gctcggggcc agagcacgcg gatccccgcg
     1921 ttcgagctcc agagcaactg cacccgcgac gggaacctca tcacctgcga gaaggtcgcc
     1981 gacctcacca ccgagatgat cgggtccacc gacgagatct tcgtcgacat cccgctctcg
     2041 gtgctccagc agcacgcggg tggctccgtt gccgggcgca cgatcggcga ggcatcgatc
     2101 tgccaaggga tctccgccca cctgtctccg gggctcgcgt tctgcggggg caccgggggg
     2161 ccgctgacga gcgacacgct caccctaagc gagacgagcg gcacctacac gatcgcctcc
     2221 aagcgggtcc gcgcgacgat cgcgcccgcc gggggtggcc cgtcgcagac cgtcgaggcg
     2281 acggtgagcc ccgacgggtc gttcctcgcc aacatctcgc ggaccggcct cgcgcccggc
     2341 acctacacgg tgacggcgca ggcctgctac gggacgaact gcgccacgaa gtcgacgacc
     2401 gcgacgatca gctgaacggg ctcagaaccg aggggtctcg gtgtactcgc cgaagatctc
     2461 cttgagcgcg gtcacgatct cgccctcggt gcagtaggcg cgagcgcagt cgatcagcgc
     2521 gggcatcatg ttcacgctct cgtcggcggc ggcggccttg agcctctcga gcgcgttgcg
     2581 cgcgttggcg tcgtcgcgct cgccccggag cctttggacg ccttcgatct gctcgacctc
     2641 gaccgcgtgc gggatcttca gcgtgtcgag cggcgtctcg tcctcgtcca cgaactcgtt
     2701 cacgccgacc ttgatgagac gccccttctc gt
//
LOCUS       DATLGG010000291         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_184946, whole genome shotgun sequence.
ACCESSION   DATLGG010000291 DATLGG010000000
VERSION     DATLGG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184946"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VM638_08280"
     CDS             complement(<1..660)
                     /locus_tag="VM638_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12456.1"
                     /translation="MRRALLSTLAAVLLGSAVPNAAAEHEPPRCPMHHTHTGPHERVS
                     CQMTLLDALEVSTEAGIGQIEVHGSTAAVVQREEGLVSLVDVSDPAAMRLLGRYDGNT
                     GRAEVDHAFDGDVAFSHDGRFLFYARQTHDFSNDGLHAVDVSDPAAPRLAHYAPGGGT
                     LRVAYHRDGDAEFVVTADAFFGIVVHRFVRTPTGGVLVPVHMNALPALKVGGPASAGL
                     FI"
     gene            complement(707..2230)
                     /locus_tag="VM638_08285"
     CDS             complement(707..2230)
                     /locus_tag="VM638_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVM12457.1"
                     /translation="MKKPTLVLVLILVLAPAQAGADTTFRFRGGGWGHGIGLSQYGAY
                     GLAEKGWSRNDILTWYYAGTSVQAVDPPKDTYRVGLLQNRGSFTLTAQDAAFDFALSS
                     APSDPIHSVGAGRTRRVVISGGRYRIYDGDKLVGAFGDADDHLLAIRRDGSRIKVEEW
                     GYRVGRGRLQFRIVSGARAHLVARVAAETYLYGLGEVPSSWPAKVLEAQAIAARSYAD
                     RIVASRSRESSCACDIYASTRDQHYMGWDKEGGYLGERWVKAVDDTARRVVAADGAPV
                     ITYYSASSGGWTEAVHRVWGGASRSYLRARCDPGDFTSANGNRTWSKELTGREAASLL
                     RARRGWGISRVTSISVLDRGPSGYVLKVRIRGRNASGDAVSFTTSGEGLRSGLALKSA
                     RVWVNANRLVTGKIRSRYDSLRCRPGLAKSARTSTNGGVWQRFAKGRIYSKDDPGTHW
                     VYGLVLRRYLDANGPRGSLGYPTTEVVRLSDGRLRTRFQNGVITCNPSTGRCSLKRT"
     gene            2345..4561
                     /locus_tag="VM638_08290"
     CDS             2345..4561
                     /locus_tag="VM638_08290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVM12458.1"
                     /translation="MARNGASFELYDVLDDAVTVQDRSGELVYANEAAASMLGFANRE
                     ALLRAQVSDVLEGFALFDEAGEPFPADQLPGRRALAGEDPPEVVVRWQRAGETGLRWS
                     AVRARPLRDERGRVLYAINTFRDVTDQRRATREVGERARQQAVVARLGHLALTEIDLT
                     DLMQEATRMVAETLDVELAKVLEVLPGGTTLLLRAGVGWKEGLVGEATVATRGSQAGH
                     TLLARRPVIVDNLATETRFSGPPLLHEHGVVSGMSVVIGGVAGSFGVLGAHTPRRRAF
                     SEDDVHFLEAIANVLATAIDRKRGDEARAELLRAEREARAQAEAARARASFLSEASVI
                     LSSSLDPEKTMQRLADLAVQRLSDWCTVDLAEDGDLRLVAIAHSDPGMVEWARDFRRR
                     FPPRRDAEVGSPKVVRTGKPEIYREIDAELIRSAARNEEHLEEIMRIAPRSAMVVPLS
                     ARGRTFGAITFLSTGAGRRYDDEDLRLAEDLAGRAALAIDNGRLYRERSHVARTLQRS
                     LLPPDLPSIPGIDLAARYRSADEAAEVGGDFYDVFPSAGDSWTAAMGDVCGRGIEAAS
                     LTGVVRQSLRAVVNHVSTPSDALTQLNGALRPQTSDMRFCTVALARLDRSNGTCRVTV
                     SIGGHPPPVILRADGRVDSVGHPGTLLGVFDELELSDAEAVLEPGDALVLYTDGLTDP
                     FEAADVPGVAGLATTLNPLAGKAAKEIAEGLERGAIEARNAPTRDDMAILVVRFAP"
BASE COUNT          643 a         1613 c         1686 g          638 t
ORIGIN      
        1 gatgaagagc ccggccgacg ccggaccgcc caccttgagc gccggcagcg cgttcatgtg
       61 gaccggcacg agcacgccgc ccgtcggcgt gcggacgaag cgatggacga cgatcccgaa
      121 gaaggcgtcg gcggtaacga cgaactcggc gtcgccgtcg cgatggtacg ccacgcgcag
      181 ggttccgccc cccggcgcgt agtgcgcgag gcgcggcgcg gcaggatccg agacatcgac
      241 cgcgtggagg ccgtcgttgg agaagtcgtg ggtctgacgc gcatagaaga ggaaccggcc
      301 gtcgtgggag aacgccacgt cgccgtcgaa ggcgtggtcg acctccgccc gtccggtgtt
      361 cccgtcgtag cgtccgagga ggcgcatcgc ggccgggtcg gagacgtcga cgagcgagac
      421 cagtccttcc tcccgctgga cgacggctgc ggtcgacccg tgcacctcga tctgcccgat
      481 gccggcctcc gtggagacct cgagcgcgtc cagcagggtc atctgacagg agacgcgttc
      541 gtgcggtccg gtgtgcgtgt ggtgcatcgg gcagcgcggg ggctcgtgct cggccgcggc
      601 gttggggacg gcggagccga ggaggaccgc ggcgagcgtc gagagcaggg ctcgtctcac
      661 ccgggggtga ttcgccgtcg ggacgcgcgg ttcctgcccg gagggctcag gtgcgcttca
      721 aggagcagcg tccggtcgag gggttgcacg tgatgacccc gttctggaac cgggtgcgga
      781 gccgtccatc gctcagccga accacctcgg tcgtggggta gccgaggctc ccgcgcgggc
      841 cgttggcgtc gaggtaccgg cgcaggacca ggccgtagac ccagtgcgtc cccgggtcgt
      901 ccttcgagta gatccggccc ttcgcgaagc gctgccagac ccccccgttc gtgctcgtgc
      961 gggccgactt cgcgaggcct ggccggcaac gcaggctgtc gtagcggctg cggatcttcc
     1021 ccgtcacgag gcggttcgcg ttcacccaca cccgcgcgct cttgagcgca agtcccgagc
     1081 gcagcccctc gccggaggtc gtgaacgaga cggcgtcacc gctcgcgttg cgaccgcgga
     1141 tccggacctt gagcacgtac ccggaggggc cgcggtccag caccgagatc gaggtgacgc
     1201 ggctgatccc ccagccgcgt cgggcgcgga gcagagacgc cgcttcgcgg ccggtcagct
     1261 ccttcgacca ggtccgattg ccgttcgcgc tggtgaagtc gcccgggtcg cagcgggccc
     1321 gcaggtagga ccgtgaggcg ccaccccaga cgcggtgcac ggcctcggtc catccgccgc
     1381 tcgacgccga gtagtacgtg atcacgggcg ccccgtcggc ggcgacgacg cggcgcgccg
     1441 tgtcgtcgac cgccttcacc cagcgctcac cgaggtagcc gccctccttg tcccagccca
     1501 tgtagtgctg gtcccgggtg ctcgcgtaga tgtcgcaggc gcaggacgac tcacggctcc
     1561 ggctcgcgac gatgcggtcg gcgtagctcc tcgcggcgat cgcctgtgcc tcgaggacct
     1621 tcgccggcca ggatgacggc acctcgccga gaccgtacag gtacgtctcg gcggcgacgc
     1681 gggccacgag gtgggcacgc gcgccggaga cgatgcggaa ctgcaggcgc cccctaccga
     1741 cgcggtagcc ccattcctcg accttgatcc gggacccgtc gcgtcggatc gcgaggaggt
     1801 ggtcatccgc gtcgccgaag gctcccacga gcttgtcgcc gtcgtagatc cggtagcggc
     1861 cccccgagat caccacccgt cgcgtgcggc ccgcccccac cgagtggatc gggtccgagg
     1921 gcgccgagct cagcgcgaag tcgaacgcag cgtcctgcgc cgtcagcgtg aacgatcccc
     1981 ggttctggag caggccgacg cggtaggtgt ccttcggcgg gtccacggcc tggaccgacg
     2041 tgccggcgta gtaccaggtc aggatgtcgt tgcggctcca ccccttctcg gccaaaccgt
     2101 aggcgccgta ctggctgagg ccgatcccgt gtccccagcc cccgccccgg aagcggaacg
     2161 tcgtgtcggc cccggcctgc gccggtgcca ggacgaggat gagcacgagg acgagcgtcg
     2221 gcttcttcat gaggaggggt gtgcggcgga tcgcgtcgcc cctcctgtat agcgcatcga
     2281 cggccctgac ggaacccctc aggtggtaca ttcccgtcga acaccggttc gcgggagggg
     2341 agaggtggcg cgcaacggag cgagcttcga gctgtacgac gtgctcgacg acgccgtcac
     2401 ggtccaggat cgttccggtg agctcgtcta cgcgaacgag gccgccgcct cgatgctcgg
     2461 cttcgcgaac cgcgaagcgc tcctgcgcgc gcaggtgtcc gatgtgctcg aggggttcgc
     2521 gctcttcgac gaggcgggag agcccttccc ggcggaccag ctcccaggac gacgcgcgct
     2581 cgccggggag gatccacccg aggtcgtcgt ccggtggcag cgcgcggggg agacgggcct
     2641 gcgctggtcg gcggtccgcg cccgcccgct ccgcgacgag cgtggccggg tcctgtacgc
     2701 gatcaacacg ttccgcgacg tcaccgatca acggcgggcg acacgcgagg tcggcgagcg
     2761 agcccgccag caggcggtcg tggcgcggct cggccacctc gccctcaccg agatcgatct
     2821 caccgacctc atgcaggagg cgacgcggat ggtcgcggag acgctcgacg tcgagctcgc
     2881 gaaggtcctc gaggtcctgc cgggcgggac gacgctgctc ctgcgggccg gggtcgggtg
     2941 gaaggagggc ctcgtcggtg aagcgacggt ggccacccga ggatcgcagg cgggccacac
     3001 gctcctcgcc cgccgcccgg tgatcgtcga caacctcgcg accgagacgc ggttcagcgg
     3061 gccgccgctc ctgcacgagc acggtgtcgt cagcgggatg agcgtcgtga tcgggggggt
     3121 ggccggatcg ttcggggtcc tcggcgcgca cacgccccga cgccgcgcgt tcagcgagga
     3181 tgacgtccac ttcctcgagg cgatcgccaa cgtcctcgcc acggccatcg accgcaagcg
     3241 tggcgacgag gcgcgcgcgg agctcctgcg ggccgagcgg gaggcgaggg cgcaggcgga
     3301 ggcggctcgc gcgcgcgcgt cgttcctgtc tgaggcgagc gtgatcctct cctccagcct
     3361 cgacccggag aagacgatgc agcgcctcgc cgacctggcg gtccagcgcc tttccgactg
     3421 gtgcacggtc gacctcgccg aggatgggga ccttcgactc gtcgccatcg cccacagcga
     3481 ccccgggatg gtggagtggg cgagggactt ccgccggagg ttcccaccgc ggcgagacgc
     3541 ggaggtgggt tccccgaagg tcgtgcgcac ggggaagcct gagatctacc gggagatcga
     3601 cgcggagctg atccggtccg cggcgcggaa cgaggagcac ctcgaggaga tcatgcggat
     3661 cgcgccgcgc tcggcgatgg tcgtgccgct gtcggcccgg ggccggacgt tcggcgcgat
     3721 cacgttcctc tccacaggcg ccggccgccg ctacgacgac gaggacctcc gactcgcgga
     3781 ggacctggcc gggcgggctg cgctcgcgat cgacaacgga cgcctgtacc gggagcggag
     3841 ccacgtcgcc cggaccctcc agcgaagcct cctgccgccg gacctgccct cgatcccggg
     3901 catcgacctg gccgcgcgct accgctccgc tgatgaggcc gcggaggtcg gcggagactt
     3961 ctacgacgtc ttcccgtcgg cgggcgacag ctggaccgcg gcgatgggtg atgtctgcgg
     4021 ccgggggatc gaggccgcat cgctcaccgg cgtggtgcgt cagtcgctcc gcgcggtggt
     4081 gaaccacgtc tcgacgccga gcgacgcact gacgcagctg aacggggcgc tgcggccgca
     4141 gacgtccgac atgcggttct gcacggtggc gctcgcccgc ctcgaccggt cgaacgggac
     4201 ctgccgcgtc accgtgtcga tcgggggcca tccgcccccg gtgatcctcc gcgcggacgg
     4261 ccgcgtcgac tccgtcggcc acccgggcac gctgctgggg gtcttcgacg agctcgagct
     4321 gagcgacgcg gaggcggtgc tcgagccggg cgacgcgctc gtgctctaca ccgacggcct
     4381 gaccgacccc ttcgaggccg ccgacgtccc cggcgtcgcg ggcctggcga cgacgttgaa
     4441 cccgctcgcc ggcaaggcgg cgaaggagat cgccgaaggc ctcgagcgtg gtgcgatcga
     4501 agcgcggaac gcccccaccc gcgacgacat ggcgatcctc gtggtgcggt tcgcgccctg
     4561 acccctcggg tcagcgagcg
//
LOCUS       DATLGG010000292         3334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_216441, whole genome shotgun sequence.
ACCESSION   DATLGG010000292 DATLGG010000000
VERSION     DATLGG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216441"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VM638_08295"
     CDS             <1..574
                     /locus_tag="VM638_08295"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVM12459.1"
                     /translation="LGARTVGDLADLPRRVLERALGEQQARHLAALAAGRDARRVVPY
                     EPPKQVSHEETFEHDVDDEARIHRELLRLSFRVAARLRAEGFRARTVSIKVRLASFTT
                     LSRSRTVADPIDAGADIDRIARELYAAIPGERRRVRLLGVAAGGLVPSGAEQLALIRS
                     GRWSDAERALDRIERRFGQGTAFPASLLEE"
     gene            627..1004
                     /locus_tag="VM638_08300"
     CDS             627..1004
                     /locus_tag="VM638_08300"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HVM12460.1"
                     /translation="MAADHDNQPLTEEELRVLEEMEQRLREEHPKLARAAASSLYAHT
                     ARRIRAGVLLFVAGFVMLMLFAMSVWVALAGFGTMVAAGMFILQQLRRLGRDHGRGWT
                     REQGFPLTAFLARVAERFRGSHR"
     gene            1140..1622
                     /locus_tag="VM638_08305"
     CDS             1140..1622
                     /locus_tag="VM638_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVM12461.1"
                     /translation="MEGFTTHQASRLSGCTPRQLRYWDQIGLVRPSLRQTGGRPGVPR
                     MYSFRDLVELRVVRSLLDGGMSLQRVRRAYEYLRRQANLDEQLSDVKLITDGKSIFKV
                     ARREGEILDALRDGQMAFFVAIDQIVRSVDAEVSEFRRDRDRFVRALRDANRQLSLEA
                     "
     gene            complement(1623..>3334)
                     /locus_tag="VM638_08310"
     CDS             complement(1623..>3334)
                     /locus_tag="VM638_08310"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HVM12462.1"
                     /translation="PRPRYAIPFLVGALVMLYATGRRRLEAWGPSGGAQRRFRDAAGR
                     HARWLAAGSLALALLAPGILPGLAAGPLVDVAGPIETQQATVSPIVDIRPSLTANRSV
                     ELFRVRSERPAYWRLLSLDRFDGRFWTAPERVVEDQMVISGPSDLPVAEPALHDDGDL
                     AAAQDGAIEQEFEILGLRAEWLPSAYLPVMSTTPGLAVWDPREATLRPVDEAPRGYRY
                     RVWSHLAAPTPADLRAIPRLADSLAVDASLPVATPPRVYAIAEALTRGEETPYEKLLA
                     IQDHLRTFAYDEAAPPGHGTSDILFFLERSRRGYCEQFAGTMAVLARALGYRARVAIG
                     FTPGNPAADGSFVVTSEHVHAWTEVYFPSIGWLAFEPTPARTNPVAGDYADPAAAIAN
                     PGGSENDQRRTQNREAGRSQRDANEGAPPDPNAPAAADRTGGMSRWWMLAALPLVPVG
                     VAIARLGRRAMRRRRARTDRDRILAAYEGFTLVAEDLGFGRRDGETPAEFAARLRAAV
                     PFSDGHLERLSALAVTALYSERTTSAEAPAVATEAERAVVRDLRRHAGAARSFAGTLG
                     LRR"
BASE COUNT          433 a         1170 c         1269 g          462 t
ORIGIN      
        1 gctcggggcc cggaccgtcg gagacctcgc cgatcttccg cgccgcgtgc tcgagcgggc
       61 gctcggggag cagcaggccc gccacctcgc ggcgctcgcg gccggccgcg atgcgcgacg
      121 ggtcgttccg tacgagccgc cgaagcaggt cagtcacgag gagacgttcg agcacgacgt
      181 cgacgacgag gcgcggatcc accgcgagct gctccggctc tcgttccggg tcgccgcccg
      241 cctgcgggcc gaggggttcc gcgcccgcac cgtctcgatc aaggtccggc tggcgagctt
      301 caccacgctc tcccgatccc gcacggtcgc ggacccgatc gacgccggcg ccgacatcga
      361 ccggatcgct cgcgagctgt acgcggcgat acccggggag cggcgacggg tgcggctgct
      421 gggggtcgcc gcgggcgggc tcgtgccgtc gggcgccgag cagctcgccc tgatccggtc
      481 gggccgctgg agcgacgccg agcgcgcgct cgaccggatc gagcgacgct tcggacaggg
      541 gacggcgttc ccggcgtcgc tcttggagga gtgagctccg ctcgcgagcg cgttcctata
      601 atggacgcga aggggtggtg agcgcgatgg ccgcagatca cgacaaccag ccgctgaccg
      661 aggaggagct ccgggtcctc gaagagatgg agcagcggct gcgggaagag cacccgaagc
      721 tggcccgcgc cgccgcgtcg tcgctctacg cccacaccgc ccggcggatc cgcgccgggg
      781 tcctgctgtt cgtcgccggc ttcgtgatgc tgatgctgtt cgcgatgtcg gtctgggtcg
      841 cgctggcggg cttcgggacg atggtcgccg ccggcatgtt catcctgcag cagctccggc
      901 ggctgggccg ggaccacggc aggggctgga cgcgcgagca gggcttcccc ctgaccgcgt
      961 tcctcgcgcg cgtggccgag cgtttccgcg gatcacaccg gtaacgatcg tctcggttcc
     1021 gtcacgcgat cacccttgac cgcgacgggt gctttcgttt atcaaatgaa ctctccttcc
     1081 cccggagcga gcgctccgtc cgcgatcgac gcgttcggtt caccggaggg ggtttcgggt
     1141 tggaagggtt cacgacgcac caggcatctc gactgtcggg ctgcacgccg cgccagctcc
     1201 gctactggga ccagatcggc ctcgtccgtc cgtcgctccg ccagacgggg gggaggccgg
     1261 gggtcccacg gatgtattcg ttccgcgacc tcgtggagct tcgggtcgtg cgatccctcc
     1321 tcgacggcgg gatgtcgctc cagcgtgtgc gccgggccta cgagtacctt cggcggcagg
     1381 cgaacctcga cgagcagctc tccgacgtga agctcatcac cgacggcaag agcatcttca
     1441 aggtcgcgcg acgggagggg gagatcctgg acgcgctccg cgatggccag atggcgttct
     1501 tcgtggcgat cgatcagatc gtgcgctccg tcgacgccga ggtgagcgag ttccgccggg
     1561 accgcgaccg cttcgtgcgg gcgctccggg acgcgaaccg gcagctctcg ctcgaggcct
     1621 gatcagcgcc gcaggccgag ggtccccgcg aacgaccgcg ccgcacccgc gtgccgccga
     1681 agatcgcgga cgacggcgcg ttccgcctcg gtcgcgaccg cgggtgcttc ggctgacgtc
     1741 gttcgctccg agtacagcgc cgtgacggcg agggcgctca gccgctccag gtgtccgtcg
     1801 gagaagggga ccgcggcccg gaggcgggcg gcgaactccg ccggcgtctc cccgtcgcgg
     1861 cgaccgaacc cgagatcctc ggcgaccagg gtgaagccct cgtaggcggc gaggatgcga
     1921 tcccggtcgg tccgtgcgcg ccgccgccgc atcgcgcgcc ggccgagacg tgcgatcgcg
     1981 acgccgaccg ggacgagcgg gagggcggcg agcatccacc agcggctcat gccgccggtg
     2041 cgatcggccg ccgcgggcgc gttcgggtcc ggtggcgccc cctcgttcgc atcgcgctgg
     2101 gaccttccgg cttcgcggtt ctgggtccgc cgttggtcgt tctcgctgcc cccggggttc
     2161 gcgatcgccg cggcgggatc ggcgtagtcg ccggcgaccg ggttcgtccg ggcgggcgtc
     2221 ggctcgaacg cgagccatcc gatcgagggg aagtagacct cggtccaggc gtggacgtgc
     2281 tcgctcgtga cgacgaagct cccgtcggcg gccgggttgc ccggggtgaa gccgatcgcc
     2341 acgcgcgccc ggtagccgag cgcgcgcgcg aggaccgcca tggtcccggc gaactgctcg
     2401 cagtagccgc gccgcgagcg ttcgaggaag aagaggatgt cgctcgtgcc gtgcccgggg
     2461 ggagcggcct cgtcgtaggc gaacgtccgc aggtggtcct ggatcgccag gagcttctcg
     2521 tagggtgtct cctcgccgcg ggtgagggcc tccgcgatcg cgtagacgcg cggcggggtc
     2581 gccacgggca ggctcgcgtc gacggcgagg gagtcggcga gccgcgggat cgcgcggaga
     2641 tccgccgggg tcggcgccgc gaggtgcgac cagacgcggt agcggtagcc acgcggggcc
     2701 tcgtccacgg ggcgcagcgt cgcctcccgc ggatcccaca cggcgaggcc cggcgtggtc
     2761 gacatcacgg ggaggtacgc ggacggcagc cactccgcgc ggagcccgag gatctcgaac
     2821 tcctgctcga tcgcaccgtc ctgcgccgcc gcgagatcgc cgtcgtcgtg cagcgctggc
     2881 tccgcgacag gcaggtcgga gggacccgag atcaccatct ggtcctcgac gacgcgctcg
     2941 ggcgccgtcc agaagcggcc gtcgaaccgg tcgagggaga ggagccgcca gtacgccggg
     3001 cgctcggagc ggacccggaa gagctcgacg ctgcggttcg cggtgaggct cgggcggatg
     3061 tcgacgatcg ggctcacggt ggcctgctgc gtctcgatcg gaccggccac atcgaccagg
     3121 gggccggccg cgaggccggg gaggatccct ggcgcgagga gcgcgagcgc gagggatccc
     3181 gccgcgagcc agcgcgcgtg ccggcccgcg gcatcgcgga agcggcgctg ggcgcccccg
     3241 ctcggccccc acgcttcgag ccggcggcgt cccgtcgcgt agagcatcac gagcgccccg
     3301 accaggaagg ggatcgcgta cctcggtcgc gggc
//
LOCUS       DATLGG010000293        10721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_234289, whole genome shotgun sequence.
ACCESSION   DATLGG010000293 DATLGG010000000
VERSION     DATLGG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234289"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VM638_08315"
     CDS             <1..699
                     /locus_tag="VM638_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVM12463.1"
                     /translation="PRERGRAGRSRTPKGQDQPRVHGPLPRGVAQEVRSLARPGKGEA
                     AVRLLDEAVSALGRERPGDAAEAAAKAKSMAPRSGAVREVLGMALYRTGRFREALTEL
                     QAYRRLTGRLDQNHLIADCHRALGSPEKAVAPAREAATSPRLPEEVRAEAAVVGASAL
                     ADLGRFDEALSLLRRHPGSGDVARPHDIRVWYVLADVLERSGRRQDAVREFRRILRHD
                     PEAYDVAERLAALA"
     gene            complement(696..1634)
                     /locus_tag="VM638_08320"
     CDS             complement(696..1634)
                     /locus_tag="VM638_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVM12464.1"
                     /translation="MAHTFAVADGIHGIDTLMFGREGATSAYLVEAREPALVETGPTT
                     SLEALLAGLADLGVGPGDLAHVIVTHIHLDHAGGAGALAPHFPDATIWVHERGAPHLS
                     DPTKLLASATRVYGAERLAEMFGSVDPVPAERLRALTDGDVIALGDRRIEAVYTPGHA
                     SHHVCLFDDRTGGVWVGDALGVFLPDVRILRPATPPPEFDLELAIESIDRVAAREPRM
                     LMFSHFGPATDVGHLCSLAVRRVRKWTAIVEEALGQTDDPKEVARILRERTQTEINPA
                     GARTEALEDRYDLLSSIEMNAMGLSRYLRKRAEAKG"
     gene            1713..2021
                     /locus_tag="VM638_08325"
     CDS             1713..2021
                     /locus_tag="VM638_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVM12465.1"
                     /translation="MITVNVVVLAGKLSADPEVVEMPSGDAVARLRVQVPEGEQRVLP
                     LPVAAWGRGPRRDCAKLAKGDLVMVRGHLVRRFFRGQGGGRSITEVVASEVRRLEDPA
                     "
     gene            2085..2597
                     /locus_tag="VM638_08330"
     CDS             2085..2597
                     /locus_tag="VM638_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12466.1"
                     /translation="MPLASRTRSDRQVRLGRIVGLVFCVAGFVVITIAWNGMASVACV
                     DCQLPYLISGGATGLGLILFGGTLLVTASIRAERLSQDARFQELSRQLSLMGAAVETQ
                     GSPDGRVVAGKSTYHRPDCRLVKGRQDLDLITVTMAAGQGLSACRVCGPEAPPAAPEA
                     ATPGEASAAR"
     gene            complement(2594..3310)
                     /locus_tag="VM638_08335"
     CDS             complement(2594..3310)
                     /locus_tag="VM638_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12467.1"
                     /translation="MALLELEDLRTGYEGTPVLHGVTLSVNEGETAVVLGLNGAGKTT
                     TMLTIAGILKPWSGRLVFDGRRIAKKDTVALVKLGIVLVPEGRHVFPGLSVMNNLRMG
                     AWSVRRDHRFVKERLAQVFEYFPILEERQGQLAGTLSGGEQQMLALGRGLMARPRLLL
                     IDEASLGLSPKLVQQVFRIVQRINEDGVTVLMVEQNAGSVRGADRAFIMEKGRIIHEG
                     AGSEMLEQGELRRAYLGTPA"
     gene            complement(3297..4073)
                     /locus_tag="VM638_08340"
     CDS             complement(3297..4073)
                     /locus_tag="VM638_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12468.1"
                     /translation="MAQLLEVRNLSIRFGGLQALQGVDLDVGEWEIVGLIGPNGAGKT
                     TFFNCVTGIYRPNTGTVKYRGRDITKLPTHRKVRLGMGRTWQNLGVSRDLTVLQNLLI
                     AQHASVRYGPLAGLFGAPGTFAEERNLRRDADEILDFLGLREVRDEVAANLPYGTLKL
                     VDLGMALATDPEILLLDEPTSGMGPDEAHKLGDTLRFLRESLDLTILMIEHHVPLVVG
                     VCDYVYVLNFGQILTRGEPQEVQRHPEVVAAYLGDQDGAA"
     gene            complement(4073..5317)
                     /locus_tag="VM638_08345"
     CDS             complement(4073..5317)
                     /locus_tag="VM638_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVM12469.1"
                     /translation="MATSTAEATAAVRRRPAAGGLAARGAVASVLVIVVLLIPVVLPS
                     FRASQATRAVIFAIIGLSLNVLLGHLGQISLGHQAFVGMGAFASGLMVGKGIPFFLAL
                     PLAGVIGALAAAILGLVALRIRGLYLALVTLAFGFVAEESIFNVNALTRGGEGMPAPR
                     PPGFLSDPAYMYLCLIVLAVVLLMDWRLVKTKGGRAIMAIRHDERVAATLGVNVTFYK
                     LFAFMLSGFLAGIAGSLFAHWITNIATNDFVLDLALVWVFMTVVGGLGSRAGIVIAAT
                     FFALLPILLEAIFPLLQLLPFINADMQGRLPRIVPVIGALLLVLTLTLYPGGIGQQLL
                     PIRRWLAGGPFRDPLKHGRKHASRPAKRRRRRGSPPTPAGDGAATTTPTEAIVDAPGP
                     AEAASAVAEAARATRPTGDGGA"
     gene            complement(5318..6190)
                     /locus_tag="VM638_08350"
     CDS             complement(5318..6190)
                     /locus_tag="VM638_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVM12470.1"
                     /translation="MIFTSILNGLITGTIYGLVALGLVLVYKGARVFNFAQGEFGTVA
                     VFVAWILHTEPPGLPMLVALLLGLAAATLMGLLVERVVVRPLSQKPRVILLVATAGVA
                     VGSIYLQLLLGEAEARVLPRLGGDRVFFVLNAALIWQNIIMILALVVIGVALYLFFSR
                     TALGLAVLASSQDNLASQLSGVSTRRVSSLVWGLAAFLGGMAGLLQAPLSVFTPGFMT
                     TTVLFPAFTGAVLGGMTSLPGAFVGGLGVGVLSQLGADYIPETIPGGRELVLFALLLL
                     VLSVRPQGLLGKEA"
     gene            complement(6212..7660)
                     /locus_tag="VM638_08355"
     CDS             complement(6212..7660)
                     /locus_tag="VM638_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM12471.1"
                     /translation="MKLRVAALAAVLSLVALACGQKPGVHARAMSEGQAGIPGAGVGV
                     GTGAVLDPTTGQYVDPTTGQVVDATTGAPVSGTTTTGGTATTTTGGAGGGGSSSSNAP
                     TGPGDRTGIYKDKIVIGLHAPVNGAAPVQSKSFETGRDLYWRYGNNGKPVRVFGKRVE
                     VSFRDDEYNPSTATQRCKQMAEGNPRAFLLIGGAGTDQIHACAKYAHGGGIPYLSAGV
                     VQNNLASFPNYFALSMTYYQQGKLLGNYIKQNRNNGNWKCDGACDKVALVITNTANFD
                     DAINGFREAYGNNFTLIRPGKRHHGFDYASRLCTATTKNFDVVYPLTSPTFFLEMMGD
                     PSASCNPQYVGVGISMGLDTVASTGCKSSRSAMNGSIFFSPAPAFTRASGSLPYGKKD
                     PVFNRYGRDDIHWLLWGLMKHIHQMFEKAGPDMSRQSFMSKLSTSRIKADVFPALRYS
                     QSNHFGASSAWILRAVCEGSGGYYITVGKVGS"
     gene            complement(7657..8877)
                     /locus_tag="VM638_08360"
     CDS             complement(7657..8877)
                     /locus_tag="VM638_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12472.1"
                     /translation="MRSARRILCLLAVAAVTVGPALPAAAQTKRDVKVPGTNWYWKSQ
                     KRAKTSDHVSCPDGTPVCPSHQQELESTHSEGSLPIKILRGEHEKYSAIEFDTSFIPF
                     QGATIHSFTFTVVESQTERDRAQTVNTDGKVIEACPIEETWPSTLEGGATMMGEAPKY
                     ATSGCVKGKRERNANPPRWRFDITKIAQGWGNQPGSAYGVMLVGAKGNDPNETWQVVL
                     RGPYRNEQSQLAGQKNVLASVSFTPKPAATGTTGTSFPSGSYSTTGTGTDPWGVGTDG
                     FGSGTTQDAPVFPEGGGAPAAPAPPAPLGAISTAAQGEEQPGGMPAYFWLLIPVALMA
                     LSLVRHTVLEPLPGKRPDGVVALIRRHNAARFGRSVTPEASSPLAGVTGTLRGTGHRI
                     RRLARRVISGGKRR"
     gene            complement(8998..10170)
                     /locus_tag="VM638_08365"
     CDS             complement(8998..10170)
                     /locus_tag="VM638_08365"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HVM12473.1"
                     /translation="MPRIAPFDGLLYSPAVDDLASVTAPPYDVILPHDLPRYHQASSR
                     NIARVDLGEGGAGTDPDDKYTEAGELLRRWQEDGTLVPTGGPAVYAYEMRFLYRARER
                     SIRGVVVEVELREWGDGVIPHERTMPGPVEDRLAHLRATAANISPIYAVVTGPCPPQT
                     ELIERAASRAPDRELVDDEGVRHRLWVERDPDPALLEWYRDRSLLIADGHHRYQTALA
                     HQRAQRGANGPGPWDAVMMLVVDAAVEEPPVLPIHRVVDVDPLPTLGTERVRDLGEIL
                     AWLSDDDLRFGAAYRHDGGLHHVVGRLGGDPPTVAALHEHLLERLPGVREVAFVHDPA
                     LAEEMVHTGRRDLALFLPPARVEHVRATVEAGRRLPEKSTYFWPKPRTGMVIRRAG"
     gene            10199..10552
                     /locus_tag="VM638_08370"
     CDS             10199..10552
                     /locus_tag="VM638_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVM12474.1"
                     /translation="MSESAYQLVARAGEFLSTGHPHQAVMLLDRAKLAEPDKGSIREA
                     LGRALYMTGSWARARREFAKAVALDPVNDYAHFALGLTCARTGERSRAIAHLKLAVAM
                     RPRDEYRRALSRLIA"
     gene            10559..>10721
                     /locus_tag="VM638_08375"
     CDS             10559..>10721
                     /locus_tag="VM638_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVM12475.1"
                     /translation="MRPADRYEGFLFDLDGVVYRGDEPIPGAAPTIATLRDLGRGLVF
                     ATNNSARTPE"
BASE COUNT         1555 a         3725 c         3851 g         1590 t
ORIGIN      
        1 cctcgggagc ggggcagggc cggccggagc cggacaccga aggggcagga tcagccgcgc
       61 gtccacgggc cgctgccgcg cggcgtcgcg caggaggtgc gctcgctcgc acggcccggg
      121 aagggcgagg ccgccgtccg cctcctcgac gaggccgtct ccgcgctcgg tcgtgagcgg
      181 ccgggggacg ccgcggaggc cgccgcgaag gcgaagtcga tggccccccg ctcgggggcg
      241 gtccgcgagg ttctcgggat ggcgctctac cggaccgggc ggtttcgcga ggcgctcacc
      301 gagctccagg cgtaccggcg actgaccggg cggctcgacc agaaccacct gatcgccgac
      361 tgtcaccgcg cgctcggatc accggagaag gctgtcgcgc ccgcccgcga ggcggcgacg
      421 agccctcgcc tgcccgagga ggtgcgggcg gaggcggcgg tcgtcggggc ctcggccctc
      481 gctgacctgg ggcggttcga cgaggcgctg tcgctcctcc ggcgccaccc cgggagcggg
      541 gacgtcgctc ggccgcacga catccgcgtc tggtacgtcc tggccgatgt cctggagcgg
      601 tccggccgac ggcaggacgc cgtgcgcgag ttccgccgga ttcttcggca cgatcccgag
      661 gcgtacgacg tggcggagcg gctcgccgcg ctcgcctagc ctttcgcctc tgcccgcttg
      721 cggaggtaac gcgagagccc catcgcgttc atctcgatgc tggagagcag gtcgtagcgg
      781 tcctcgagcg cctccgtccg ggcacccgcg gggttgatct ccgtctgggt gcgctctcgg
      841 aggatgcgcg cgacctcctt ggggtcgtcc gtctggccga gcgcttcctc gacgatcgcc
      901 gtccacttcc ggacccggcg caccgcgagg ctgcacaggt gtccgacatc cgtcgcgggt
      961 ccgaaatggc tgaacatcag catccggggc tctcgcgccg cgacccggtc gatgctctcg
     1021 atcgccagct cgaggtcgaa ctccggcggc ggcgtcgccg ggcgcaggat cctcacgtcg
     1081 gggaggaaga cgcccaaggc gtcgccgacc cagacgccgc cggtgcggtc gtcgaagagg
     1141 cacacgtgat ggctcgcgtg ccccggggtg taaacggcct cgatgcgtcg gtctcccagg
     1201 gcgatcacgt ccccatcggt gagggcgcgg agccgctcgg ccgggacggg gtccactgag
     1261 ccgaacatct cggcgagccg ctcagctcca tagacgcgcg tcgcgctcgc caggagcttc
     1321 gtcgggtccg agaggtgggg cgcgccgcgc tcgtggaccc agatcgtcgc gtcggggaag
     1381 tggggagcga gggcccccgc gcctccggcg tggtcgagat ggatgtgcgt gacgatcacg
     1441 tgcgccagat ccccgggccc gaccccgagg tcggcgaggc ccgccaggag cgcttcgagg
     1501 gaggtcgtgg gccccgtctc gacgagcgcg ggttcccgcg cctccaccag gtacgcggag
     1561 gtcgcgccct cgcggccgaa catgagcgtg tcgatcccgt ggatcccgtc cgcgacggcg
     1621 aaggtgtgcg ccacggggcg gagtctagcc gtccgtgtcg gtggctgccg ctagcgttgg
     1681 ccgtggggag cccggcgggg aaggagaccg agatgatcac ggtcaacgtg gtggtcctcg
     1741 ccggcaagct gtcggcggac ccggaggtgg tggagatgcc ttccggcgac gcggtcgccc
     1801 ggctgcgcgt ccaggtgccg gagggggagc agcgtgtcct gccgcttccg gtcgccgcct
     1861 gggggcgcgg acctcggcgg gactgcgcga agctggccaa gggagacctc gtgatggtcc
     1921 gcgggcatct cgtccgtcgc ttcttccggg gccagggggg cggccgctcg atcaccgagg
     1981 tggtggcgtc ggaggtgcgg cgtctggagg acccggcgta gccgccttcc ctcccacgag
     2041 ctcgggaata gccctctcat actcgataga atgtcgggct ggaggtgccg ttggccagcc
     2101 gcacgcgatc cgatcgccag gtccgcctcg gccgcatcgt cggcctcgtc ttctgcgtgg
     2161 ccgggttcgt cgtcatcacg atcgcctgga acggcatggc gagcgtggcg tgcgtcgact
     2221 gccagctgcc ctacctcatc tcgggcggcg ccaccggtct cgggctcatc ctgttcggag
     2281 gcaccttgct ggtgacggcc tcgatccgtg ccgagcgcct gagccaggac gcgcggttcc
     2341 aggagctctc gcggcagctc tctctgatgg gcgcggccgt ggagacgcag gggtcgccgg
     2401 atggccgcgt cgtggcgggg aagtcgacct accaccgtcc cgattgccgg ctcgtcaagg
     2461 gacggcagga cctcgacctc atcacggtga ccatggccgc cgggcaaggg ctgtccgcgt
     2521 gccgggtctg cgggcccgag gctccgccgg cggccccgga ggccgcgacg ccaggcgaag
     2581 cctcagccgc gcgctaggcc ggggtgccga ggtaggcgcg ccgaagctcg ccctgctcga
     2641 gcatctcgct gccggcgccc tcgtggatga tccggccctt ctccatgatg aacgcgcggt
     2701 cggccccacg gaccgatccc gcgttctgct cgaccatcaa gacggtcacg ccgtcctcgt
     2761 tgatccgctg gacgatccgg aagacctgct ggaccagctt cggtgagagg cccagcgagg
     2821 cctcgtcgat cagcagcagc cgtggccgcg ccatcaaccc tcgaccgagc gcgagcatct
     2881 gctgctcgcc gccggagagg gtcccggcga gctgtccctg ccgctcctcg aggatcggga
     2941 agtactcgaa gacctgcgcg agccgctcct tcacgaaccg atgatcccga cggaccgacc
     3001 aggcgcccat ccgcaggttg ttcatgaccg aaaggcccgg gaagacgtgc cgtccctccg
     3061 gcaccaggac gatccccagc ttgaccaggg cgacggtgtc cttcttcgcg atcctgcggc
     3121 cgtcgaagac cagccgcccg gaccagggct tgaggatgcc ggcgatcgtc agcatcgtgg
     3181 tcgtcttgcc cgccccgttc agtcccagca ccacggccgt ctcgccctcg ttcaccgaga
     3241 gcgtcacccc gtgcaatacc ggggtccctt cgtaaccggt tcgcaggtcc tcgagctcaa
     3301 gcagcgccat cttggtctcc caggtaggcc gcgacgacct cgggatgccg ctggacctcc
     3361 tgcggctcgc cgcgggtcag gatctggccg aagttcagca cgtacacgta gtcgcacacc
     3421 ccgacgacca gcgggacgtg gtgctcgatc atgaggatcg tgaggtcgag cgactcccgg
     3481 aggaaacgga gcgtgtcacc gagcttgtgg gcctcgtccg gccccatccc cgaggtgggc
     3541 tcgtccagga gcaggatctc cgggtcggtg gcgagggcca tcccgaggtc gacgagcttc
     3601 agcgtgccgt agggcaggtt ggccgccacc tcgtcgcgga cctcgcggag ccccaggaag
     3661 tcgaggatct cgtcggcgtc gcggcggagg ttccgctcct ccgcgaaggt tccgggggcc
     3721 ccgaacagcc ccgccagcgg gccgtagcgg acgctcgcgt gctgggcgat gagcaggttc
     3781 tgcaggacgg tgaggtcacg agagacgccg aggttctgcc acgtccgacc catgccgagg
     3841 cgcaccttgc ggtgggtcgg gagcttcgtg atgtccctgc cgcgatactt cacggtgccg
     3901 gtgttcggac ggtagatccc cgtcacgcag ttgaagaagg tggtcttccc ggcgccgttc
     3961 gggccgatga gcccgacgat ctcccactcg ccgacgtcga ggtcaacgcc ctggagcgcc
     4021 tggagcccgc cgaaccggat ggagaggttc cggacctcga ggagctgtgc catcaggccc
     4081 caccgtcccc ggtcgggcgg gtcgcacgtg cggcctccgc cacggcgctc gccgcctctg
     4141 ccggacccgg tgcatcgacg atcgcctcgg tcggcgtcgt ggtcgccgcg ccgtcgcctg
     4201 cgggcgtcgg gggagaccct ctgcgccgtc ggcgcttcgc cggccgcgac gcgtgcttcc
     4261 gtccgtgctt cagcggatcg cggaagggcc cgccggcgag ccatcgcctg atcggcagga
     4321 gctgctggcc gatcccgccc gggtacagcg tgagcgtcag cacgagcagc agcgccccga
     4381 tgaccgggac gatgcgtggc aggcgcccct gcatgtccgc gttgatgaag gggaggagct
     4441 ggagcagcgg gaagatcgcc tcgagcagga tcggcaggag cgcgaagaac gtggcggcga
     4501 tcacgatccc cgcgcgcgat ccgagccccc cgacgaccgt catgaacacc cacacgagcg
     4561 cgaggtccaa gacgaagtcg ttcgtggcga tgttcgtgat ccagtgggcg aacaggcttc
     4621 ccgcgatccc ggcgaggaag cccgacagca tgaaggcgaa gagcttgtag aacgtgacgt
     4681 tcacgccgag cgtggccgcg acgcgctcgt cgtggcggat cgccatgatc gctcggcccc
     4741 ccttcgtctt cacgagccgc cagtccatca gcaggacgac cgcaaggacg atcagacaga
     4801 ggtacatgta cgccgggtcg ctcaggaagc ccggcggccg cggcgccggc atcccctcgc
     4861 cgccgcgggt gagcgcgttc acgttgaaga tcgattcctc ggcaacgaac ccgaacgcga
     4921 gggtgacgag cgcgaggtac agtccccgga tccggagcgc caccagcccc aatatcgcag
     4981 cggcgagcgc cccgatgacg ccggcgagcg gcaaggccag gaagaacggg atccccttgc
     5041 cgaccatcag ccccgaggcg aaggctccca tgcccacgaa ggcctgatga ccgagggaga
     5101 tctggccgag atgacccagc aggacgttga gcgacagtcc gatgatcgcg aagatcaccg
     5161 ctcgggtcgc ctggctcgcg cggaacgacg gcaggacgac ggggatcagc aggacgacga
     5221 tcaccaacac cgacgcgacg gcgccgcgtg cggcgagacc gcctgccgcc gggcgtcgcc
     5281 gaacggccgc ggtcgcctcg gcggtggagg tcgccaccta cgcctccttc ccgaggagac
     5341 cctgcggccg gaccgacagc acgagcagga ggagggcgaa caggacgagc tcgcgtccgc
     5401 cggggatcgt ctcggggatg tagtccgcgc cgagctggct gagcaccccg accccgaggc
     5461 ccccgacgaa ggcgcccggc aggctggtca tcccgccgag gacggcaccg gtgaaggccg
     5521 ggaacagcac ggtcgtggtc atgaagcccg gagtgaacac cgacaacggg gcctggagca
     5581 atccggccat gcctccgagg aaggccgcga gcccccaaac gagcgaggag acccggcgcg
     5641 tgctcacgcc actgagctgg ctcgccaggt tgtcctgcga cgaggcgagc accgcgaggc
     5701 cgagcgccgt gcgggagaag aacaggtaga gcgcgacccc gatcacgacg agggcgagga
     5761 tcatgatgat gttctgccag atcagcgccg cgttcaggac gaagaagacc cggtcgccgc
     5821 cgagcctggg caggacccgc gcctcggcct cgccgagcag cagctggagg tagatcgacc
     5881 ccacggcgac acccgcggtc gcgaccagca ggatcaccct cggcttctgg ctgagagggc
     5941 ggaccacgac ccgctccacg aggaggccca tcagggtcgc cgccgcgagc ccgagcagca
     6001 gcgcgaccag catcggcaac ccgggcggct cggtgtgcag gatccacgcg acgaacaccg
     6061 ccaccgtgcc gaactcacct tgggcgaagt tgaagacccg cgcgcccttg tagacgagaa
     6121 ccagaccgag cgcgaccagg ccgtagatcg tcccggtgat cagcccgttg aggatcgagg
     6181 tgaagatcat ccggtcactg ccggcccggg atcaactccc aaccttcccc accgtgatgt
     6241 agtagccgcc gcttccctcg cacaccgccc gaaggatcca ggcgctgctc gccccgaagt
     6301 ggttgctctg gctgtagcgc agcgccggga agacgtcggc cttgatccgg gaggtgctga
     6361 gcttggacat gaagctctgg cgcgacatgt cgggccccgc cttctcgaac atctgatgga
     6421 tgtgcttcat caggccccag agcaaccagt ggatgtcgtc ccgtccgtac cggttgaaga
     6481 ccgggtcctt cttaccgtag gggaggcttc ccgaggcccg ggtgaacgcg ggcgccggag
     6541 agaagaagat cgagccgttc atggcggagc gcgaggactt gcagcccgtg gaggcgacgg
     6601 tgtccaggcc catcgagatg ccgacgccga cgtactgcgg gttgcaggag gcgctcgggt
     6661 cccccatcat ctcgaggaag aacgtcggcg acgtgagtgg gtagacgacg tcgaagttct
     6721 tcgtggtggc ggtgcacagg cggctcgcgt agtcgaagcc gtgatggcgc ttcccgggcc
     6781 ggatcagcgt gaagttgttg ccgtacgcct cgcggaaccc gttgatggcg tcatcgaagt
     6841 tcgccgtgtt cgtgatcacg agggcgacct tgtcgcaggc gccgtcgcac ttccagttgc
     6901 cgttgttgcg gttctgcttg atgtagttgc cgagcagctt gccctgctgg tagtaggtca
     6961 tcgacagggc gaagtagttc gggaagctgg cgaggttgtt ctgaacgacc ccggccgaca
     7021 ggtaggggat gcccccgccg tgtgcgtact tcgcgcaggc gtggatctgg tcggtcccgg
     7081 ctcccccgat caacaggaag gcccgcgggt tgccctccgc catctgcttg catcgctggg
     7141 tcgccgtcga ggggttgtat tcgtcgtccc tgaaggacac ctcgacccgc ttgccgaaca
     7201 cacgcaccgg cttgccgttg ttcccgtagc gccagtacag atcccgtccg gtttcgaagg
     7261 acttgctctg aaccggggcg gccccgttca ccggcgcgtg gagaccgatc acgatcttgt
     7321 ccttgtagat gccggtgcgg tcgccggggc ccgtgggcgc gttgctcgat gacgatccgc
     7381 cgccgccggc gcctcccgtc gtcgtggtgg cggtcccacc ggtcgtcgtg gttccgctga
     7441 ccggcgcgcc cgtcgtggcg tcgacgacct gccccgtcgt cgggtcgacg tactgacccg
     7501 tcgtggggtc gaggacggcg ccggtcccga cgccgacgcc ggccccaggg atgcccgcct
     7561 ggccctcgct catcgctcgc gcatggacgc ccggcttctg cccgcaggcg agcgcgacga
     7621 gggacaggac agcggcgagg gcagcgacgc gtagcttcat cgccgcttcc cgccggagat
     7681 cacgcgtcgt gccagtcggc ggatccggtg gccggtcccg cgcagggtcc ccgtgactcc
     7741 cgcgagcggg ctcgaggcct cgggcgtgac gcttcgcccg aagcgggcgg cgttgtgccg
     7801 acggatcagc gcgacgacgc cgtccgggcg cttgcccggg agcggctcga gcacggtgtg
     7861 gcggacgagg gagagggcca tgagcgcgac ggggatgagc agccagaagt aggcgggcat
     7921 acccccgggc tgctcctcac cctgcgcagc ggtcgagatc gcgccgagcg gcgccggggg
     7981 agcaggggcc gcgggggccc cgccaccctc tgggaagacc ggggcgtcct gcgtcgtgcc
     8041 ggatccgaag ccgtccgttc ccacgcccca tggatcggtc ccggtccccg tcgtggagta
     8101 ggacccgctc gggaagctcg tgccggtggt tccggttgcg gccggcttcg gggtgaagga
     8161 caccgaggcg agcacgttct tctgaccggc gagctgcgac tgctcgttgc ggtacggacc
     8221 gcggaggacg acctgccatg tctcgttcgg atcgttgccc ttcgccccga ccagcatcac
     8281 gccgtacgcc gagcccggct ggttgcccca gccctgggcg atcttcgtga tgtcgaagcg
     8341 ccaccgaggc gggttcgcgt tgcgctcccg cttgcccttg acgcagccgg aggtcgcgta
     8401 cttcggcgcc tcgcccatca tcgttgctcc gccctcgagg gtgctcggcc aggtctcctc
     8461 gatcggacac gcctcgatga ccttcccgtc ggtgttcacc gtctgtgcgc gatcccgctc
     8521 cgtctgggac tcgacgacgg tgaacgtgaa gctgtggatc gtcgcgccct ggaacgggat
     8581 gaacgacgtg tcgaactcga tggccgagta cttctcgtgc tccccgcgga ggatcttgat
     8641 cggcagtgat ccctcgctgt gcgtgctctc cagctcctgc tgatgcgagg ggcagaccgg
     8701 ggtgccgtca gggcagctca cgtggtccga cgtcttggcc cgcttctggg acttccagta
     8761 ccagttcgtg cccggaacct tgacgtcccg cttcgtctgg gctgccgcgg gcagggcggg
     8821 accgacggtc acggcggcga ccgccagcag gcacaggatc cgtcgcgcgc tcctcatctc
     8881 gctctcctcg gcggggctcc tccaagcatc ctaccgctcc tggcgcgtgg cggcaggggc
     8941 gtccgacccc tgttcctatg ttggacggcg aggcccgaac tcctgcctcg cccttgatta
     9001 ccccgctcgg cggatcacca tcccggtccg gggcttgggc cagaagtagg tggacttctc
     9061 cgggaggcgc cggccggcct cgacggtggc ccgcacgtgc tcgaccctgg cgggcgggag
     9121 gaagagggcg aggtcccgac ggccggtgtg caccatctcc tcggccagcg ctgggtcgtg
     9181 cacgaacgcg acctcgcgga ccccgggaag tcgctccagg aggtgctcgt ggagcgccgc
     9241 caccgtgggt ggatccccgc cgaggcggcc gacgacgtgg tggaggccgc cgtcgtgacg
     9301 gtacgccgcc ccgaagcgga ggtcgtcgtc cgagagccac gcgaggatct cccccagatc
     9361 gcggacccgc tcggtcccga gcgtcgggag ggggtcgacg tcgacgacgc ggtggatcgg
     9421 caggacgggt ggctcctcca cggccgcatc cacgacgagc atcatcacgg cgtcccaggg
     9481 acccgggccg ttcgctccgc gctgggcgcg ctggtgcgcg agcgccgtct ggtagcggtg
     9541 atgcccgtcg gcgatcaaca gcgagcggtc gcggtaccac tcgaggagcg cggggtcggg
     9601 gtcccgctcg acccatagcc ggtgacgcac cccctcgtcg tcgaccagct cgcggtcggg
     9661 ggcgcggctc gccgctcgct cgatcagctc cgtctgcggc gggcaggggc cggtcaccac
     9721 cgcgtagatc ggtgagatgt tggccgccgt cgcccggagg tgggcgagcc ggtcctcgac
     9781 cgggcccggc atcgtgcgct cgtgggggat gaccccgtcc ccccactcgc ggagctccac
     9841 ctccaccacc acgccgcgga tcgagcgctc gcgtgcccgg tagaggaacc gcatctcgta
     9901 ggcgtagacg gcggggcccc cggtcgggac cagggtcccg tcctcctgcc atcgacggag
     9961 cagctcgccg gcctccgtat acttgtcgtc ggggtccgtg ccggcgccgc cctcgcccag
    10021 gtcgacccgc gcgatgttgc gggagctcgc ctggtggtac cggggaaggt cgtggggaag
    10081 gatgacgtcg tacggcgggg cggtcaccga ggcgaggtcg tcgactgcgg gcgaatacag
    10141 cagcccgtcg aagggagcga tccgcggcac gggacctgta tagaccaccg gagggaccat
    10201 gagcgaatcg gcctaccagc tcgtcgcgag ggcgggggag ttcctctcga cggggcaccc
    10261 gcaccaggcg gtgatgctcc tggaccgggc gaagctggcc gagccggaca aggggtcgat
    10321 ccgagaggcc ctcggccggg cgctctacat gacggggagc tgggcgcgcg cccggcgcga
    10381 gttcgccaag gcggtcgcgc tggacccggt caacgactac gcgcacttcg ccctcggcct
    10441 gacctgcgcg cgcaccggtg agcgctcgcg cgcgatcgcc catctcaagc tcgcggtggc
    10501 gatgcggccg cgcgacgagt accgccgggc gctctcccgc ctcatcgcct aggcgcggat
    10561 gcgcccggcc gatcgctacg aaggcttcct cttcgacctc gacggcgtcg tctaccgcgg
    10621 cgacgagccg atcccaggtg ccgcgccgac gatcgccacg ctgcgcgacc tcgggcgggg
    10681 cctcgtcttc gccacgaaca actccgcgcg cacccccgag c
//
LOCUS       DATLGG010000294         7641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_13198, whole genome shotgun sequence.
ACCESSION   DATLGG010000294 DATLGG010000000
VERSION     DATLGG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13198"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VM638_08380"
     CDS             complement(<1..1485)
                     /locus_tag="VM638_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12476.1"
                     /translation="MADPGPPRGFRERLETFPTSHAPVRDFRRLIRSAAVELAALDRP
                     SLSADHDFSDFRVLVPAGDLDGDGREDALDLSERTVAGVKGTNGATIWNQDYDGWGIL
                     LGTGALDALAGTDLITLEVLERDINAGYHWTMRVRGRSGRTGQILWTRSYENLWTVGR
                     LGSTTGYVDTDFALPLGVGKLSTDAAPDVLVGRYNIARTSTPGASESGIATLFEAVSG
                     ANGVPLGSFPGAGRFGVPDAVIVPDLSNDGADDVVTVSVVRSTRHSSFTARLSAFPGS
                     GGAPVWRRDIGGMFEQPYLVGHHLNGDGRGDLLVVEYGWYALVQALSGTNGSTLWADA
                     YWGDPWITAAGSATGPGQDLLMISEGSGFGVFAAALPTTSPDPTAKVRAPELAPSLGL
                     PALPGHDVVSSPYGCDGEDCPDPCYGYGPGCPPEPCYGGGYGCEPSCPGGYGYAYAYS
                     GGGGCSVTAVTLIEGATGEWEWNREILGGYSEPAGDATGDGVVDV"
     gene            1766..3655
                     /locus_tag="VM638_08385"
     CDS             1766..3655
                     /locus_tag="VM638_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12477.1"
                     /translation="MSPRRAVAALGLAAIAVALFYLSIYPIRGYRQPLGFDTARYLWR
                     NRCVADAGLAGLRACGPPLATSMPGRIAYPLVTLPARTVLGVDRATAAAVVPAVFSLG
                     MGLAAAALLRPALRVDPVTTAVAVVTVATSTIAVRLAAPESYADNLIALGLGTAALAL
                     AAGRNLGPSARVTAAVMLGAAGLSHWPTLALITLVLLAGAALLLPGAWRDRRGESIHR
                     SAAARFAAVAALGLALWIAATTLLLGTGPDRFFAAPMASRAKLVLDRQAHSFPITLPA
                     AMIGVVALRRRRSEGRAELEALLAGWVLVVVAALVRWALAPALPELLRAFGSGFPPHR
                     FVAMALPLPMLGAVAIAWTRGIGRGVGTALLLFAIAGWMVIGGRSWWRSEPFLSTPRL
                     TEAAVLARYLDRQVPAGVPVIVPVDGVGVNAFTDLYQLADNLRAELPPSRIPNAYLYL
                     GRTEQLLAGQPTFVGDTGYDAASRHRWSAVRRVFRGPHVVALLRSFSPEVQRVAAQHP
                     TRRLARDVLILRGPTPITLGMGAPVAEVRSVLLAWAYTGMLVLLGLLGWGWSGRLARQ
                     DLLLRLALAPAVGTGVLVWGGLLADASGISPGGLSGTVLLAGLTAGGLWWGRRSSVDG
                     NEPPS"
     gene            complement(3690..6002)
                     /locus_tag="VM638_08390"
     CDS             complement(3690..6002)
                     /locus_tag="VM638_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12478.1"
                     /translation="MRRFLRAATISLTVVGGLALGVPNASATPVCTDGFMGGPPRAAC
                     GGRVFPEAAIARDYVQFLPQPGTQFKEFEAGIKYLAQLYPRWVSVFNLRQHYGDDDAV
                     SVGPDLKRSHDPTDTGDGYDIWVVKLTDHQVPDDGKHTMLFSLSVHGDEKGGIEGGLR
                     AVEDLAMGAERNDPARDRIADGVPGYESTTGVDPDFHSYPIRTILRKQAVYFVNFNMD
                     GWARGDQANWPGLYSRGNYLNTDLNRQMPTVGYINPSRNPLFESEMNFGHRFMHEVAE
                     AGKGELMAYGADIHGESQSRAWADIMYPAGQFDSIKHRQLMAIAERTKSVMDDTLFLG
                     LVNLGEEATGGDAGEGIEDHHGSIPKNTVMTKPARWGTVWDTLGYTDTGFIGDYMANE
                     LGVTGMDYEIALNHADSYTSIRAAGKPWSVVLQENYINASRAIIKTAMAYAMFQEQEF
                     ADFQIDPKGKVGYVFDPVRVRHDDENGAGRLPGPSGNGVGQNGRPVAQRPYSASNMDW
                     FEDTAKYVKGGMAKLTPFEIARDGAALDDVDTLVIADQIAPPDALGRSYDRAAYVANL
                     RAWVERGGNLVLTDRAIHALGEMGVLPAAAITNVNVYQPYSNIRNFGHPMVQGLRKNA
                     RQLAEYTLIGYGIGNSASPMTRVTKTAWESAGGVTVGTVGNNAGTSDDQTQVAVGELP
                     LGAGKVRITNGLGKPTEANDHRYGLKDYALTYSGLFILENSIVHDAEGLGGEAATPSQ
                     ESGSKAPTDGLIPFLGLAPVAAAMRLRRRR"
     gene            complement(6119..6192)
                     /locus_tag="VM638_08395"
     tRNA            complement(6119..6192)
                     /locus_tag="VM638_08395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6156..6158),aa:Thr,seq:tgt)
     gene            complement(6198..6908)
                     /gene="sigH"
                     /locus_tag="VM638_08400"
     CDS             complement(6198..6908)
                     /gene="sigH"
                     /locus_tag="VM638_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HVM12479.1"
                     /translation="MAVASTATGPYLEATEVSDEELVLSYRHGDQRAIEVLLSRYRNF
                     ARMKARSYFLVGADRDDIVQEGMIGLYKAIRDFSASKQSAFRAFAEICITRQIITAIK
                     TATRQKHGPLNTYVSLSKPVATDGDADRVLVDVLKSVQTSDPADMVISNEELASIKVA
                     FSEILSEFESEVLRLYVEGKTYHEISDYLGRHVKSIDNALQRIKRKVDLHLRARAKRN
                     RAEERDAASPAAPTAPVH"
     gene            complement(7075..>7641)
                     /locus_tag="VM638_08405"
     CDS             complement(7075..>7641)
                     /locus_tag="VM638_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HVM12480.1"
                     /translation="LDRLAQGPPHQGVVARVRLPAELGEADLAGREWPGSALALVLDG
                     VTDPQNLGAAARGAEAAGASVLVSRRHRGAAVSAAAVRASAGALLHLPMARVANIGRA
                     IQRLQEAGFWSIGLDADAPRRIDELDPPDGRLAVVAGAEGPGLSRLVRERCDELASIP
                     MLGRVDSLNVAVASALALYVLGPMARKN"
BASE COUNT         1052 a         2650 c         2677 g         1262 t
ORIGIN      
        1 cacgtccacc acgccgtcgc cggtggcgtc gccggccggt tcggagtacc caccgaggat
       61 ctcgcggttc cactcccact cgcccgtcgc tccctcgatg agcgtcaccg ccgtcaccga
      121 gcacccgcca ccgccggagt aagcgtaggc gtagccgtag ccgcccgggc aggagggctc
      181 gcatccgtat ccgccgccgt agcacggctc cggcgggcac cccggaccgt agccgtaaca
      241 ggggtcgggg cagtcttcgc catcgcagcc atacggtgac gagaccacgt cgtgtcccgg
      301 cagcgccggc agacccagcg acggggccag ctcaggcgcc ctgaccttgg cggtcgggtc
      361 tggtgaggtc gtcggcagcg cggcggcgaa caccccgaag ccggagccct ccgagatcat
      421 gaggagatcc tggcccggtc cggttgcgct tcccgccgcg gtgatccacg gatcccccca
      481 gtaggcgtcc gcccagaggg tcgacccgtt cgtgccggag agcgcctgca cgagcgcgta
      541 ccacccgtac tcgacgacca gcaggtctcc acgaccgtca ccgttcaggt ggtgcccgac
      601 gaggtagggc tgctcgaaca tgccgccgat gtcgcgccgc cacaccggtg ccccgccgct
      661 gcctgggaac gcagaaaggc gcgcggtgaa ggatgagtgg cgagtggagc gaacgacgga
      721 aaccgtcaca acgtcgtcag caccgtcgtt cgagagatct ggaacgatga ccgcgtctgg
      781 aaccccgaac cgtccggcac cggggaacga tcccagcggg acaccgttcg cgccggagac
      841 agcttcgaac agcgtcgcga tgcctgattc cgaggcgccc ggcgtcgagg tgcgcgcgat
      901 gttgtaccga ccgaccagga catcgggggc ggcatcggtc gacagcttcc caacacccag
      961 cgggagcgcg aagtcggtgt cgacgtagcc ggtcgtcgaa ccgaggcggc cgacggtcca
     1021 caggttctca taggacctcg tccagaggat ctgtccggtg cggcccgaac gaccccgtac
     1081 gcgcatggtc cagtggtacc ccgcgttgat gtcacgctcg aggacctcga gcgtgatgag
     1141 atcggtcccg gcgagagcgt cgagcgcgcc cgtcccgagc aggattcccc acccgtcgta
     1201 gtcctggttc cagatcgtgg cgccgttcgt gcccttgacc ccggccacgg tcctctccga
     1261 aagatcgagc gcgtcctctc gcccgtcccc gtcgaggtcg cccgcaggca cgaggacccg
     1321 gaagtctgag aagtcgtggt cggcagagag actcggccgg tcgagcgcag cgagctcgac
     1381 ggcggccgaa cgaatcagcc ggcggaagtc gcgcaccggc gcgtgggatg ttgggaaggt
     1441 ctcgagccgc tcccggaaac cgcgcggcgg accgggatcc gccatccgac ttgcgcgcgc
     1501 ctgcttcgtc ctatcctcac tgccgccgaa tgcgccggcc tgtgccatcg tcgcgacagc
     1561 gagcagtgag accagcgtcc atgagagcca cgatgatgag ggcttgcccg cgggcatcgg
     1621 tcctcccttc gatggcggga gggaggccac gtcggaccga tcaccctccc gcgttgctgc
     1681 aggtattcgc tgtgggagac ggccatcctt tgtgcatgtg cacaaagaca gcgggtcgag
     1741 cggccgatgg tacgctccgc ccgatatgtc gccccgccgg gctgtcgcgg ccctgggact
     1801 cgccgccatc gcggtggcgc tcttctacct gagcatctac ccgatccggg gctaccggca
     1861 gccgctcggc ttcgacaccg cgcgctacct gtggcggaac cgctgcgtcg ccgacgccgg
     1921 gctcgccgga ctgcgagcgt gtggaccacc gctggcgaca tcgatgccgg gccggatcgc
     1981 ctacccgctc gtgacgctcc cggccaggac cgtcctcggg gtcgatcgtg ccaccgcggc
     2041 ggctgttgtg cctgcggtct tctcactggg gatgggactg gcggcggcag ccctgctgcg
     2101 ccccgccctg cgcgtggacc ctgtaacgac cgcggttgcc gtcgtcaccg tcgccacctc
     2161 gacgatcgcc gtgcgcctcg ccgcaccgga gagctacgcg gacaacctga tcgccctcgg
     2221 cctcgggacg gccgcgctcg cgttggccgc ggggcggaac ctcggaccat ccgcccgcgt
     2281 gacggccgcc gtgatgcttg gcgcggcggg tctctcccat tggccgacgc tcgccttgat
     2341 cacgctcgtt ctcttggccg gcgccgcgct gctcctgccc ggggcttggc gcgatcgacg
     2401 gggcgagtcg atccatcggt cggccgcggc acgcttcgcc gcggttgccg ccctcgggct
     2461 cgccctgtgg atcgcggcga cgacgctgct cctcgggacg gggcccgacc ggttcttcgc
     2521 cgcgcccatg gcctcacgcg caaagctggt gctcgatcgc caggcgcact cgttccccat
     2581 cacgcttccg gcagcgatga tcggcgtcgt cgcgctgcga cggcggcgaa gcgagggacg
     2641 cgccgagctc gaggccctgc tcgccggctg ggtcttggtc gtcgtcgcgg cgctcgtgag
     2701 atgggcgctc gcaccggcgc tgcccgagct cctgcgagca ttcgggtcgg gtttcccgcc
     2761 gcatcgtttc gtcgcgatgg ccctgccgct cccgatgttg ggcgcggtcg cgatcgcttg
     2821 gacgcggggg atcgggcgcg gagtcggaac ggcgctgctg ctgttcgcga tcgctggatg
     2881 gatggtgatc gggggccggt cgtggtggcg gtcggagccg ttcctctcca cgccacgtct
     2941 caccgaggcg gcggtgcttg cacggtacct ggaccgtcag gtcccggcgg gggttccggt
     3001 catcgtcccc gtcgacggcg tgggcgtcaa cgcgttcacc gacctgtatc agctcgcgga
     3061 caacctccgc gcggagctgc caccctcgcg gatcccgaac gcttacctct atctcggacg
     3121 gaccgagcag ctgctcgcgg gacagccgac gttcgtggga gacactggct acgacgcggc
     3181 ctcgcgccac cgctggagcg cggtccgcag ggttttccgg gggccgcacg tcgtggcact
     3241 gcttcgatcg ttctcgccag aggtccagcg cgtcgcggcg cagcacccga cgcgccgtct
     3301 cgcccgtgac gtcctgatcc tgcgggggcc gacccccatc accctcggga tgggcgctcc
     3361 ggtcgcggag gtccgctcgg tgcttctggc gtgggcgtat acggggatgc tcgtgctcct
     3421 cggcctcctc gggtgggggt ggagcggtcg gctggcccgc caggatctgc tcctgcgtct
     3481 ggccttggcg cctgccgttg ggacgggggt cctcgtatgg ggcggcctgc tcgcggacgc
     3541 gagcggcatc tcgccggggg gcctctccgg caccgtgctt ctggcgggac tcacagccgg
     3601 tgggctctgg tgggggcgcc ggtcgagcgt ggacgggaac gagcccccga gctgatgccc
     3661 gggggctcgt ccgatcggag cggcttcgat cagcggcggc gccggagcct catcgcggcg
     3721 gccacggggg cgagaccgag gaacgggatc agcccgtccg tcggtgcctt ggaaccgctc
     3781 tcctggctcg gcgtggcggc ctctcctccc agcccctcgg cgtcgtggac gatcgagttc
     3841 tcgaggatga acagccccga gtaggtcagg gcgtagtcct tcaggccgta gcggtggtcg
     3901 ttcgcctccg tcggcttccc gaggccgttc gtgatccgga ccttcccagc cccgaggggg
     3961 agctccccaa ccgccacctg cgtctggtcg tcggaggtgc cggcgttgtt gccgacggtg
     4021 ccgaccgtga ccccgcctgc ggactcccac gccgtcttgg tgacccgtgt catgggggag
     4081 gcgctgttgc cgatcccgta gccgatgagc gtgtactccg cgagctgccg cgcgttcttc
     4141 cgcagaccct gcaccatcgg gtggccgaag ttccggatgt tcgagtacgg ctggtacacg
     4201 ttgacgttgg tgatcgctgc tgccggcagg acccccatct cgccgagcgc gtggatcgcg
     4261 cgatcagtga ggacgaggtt gccgccacgc tcgacccagg cccgcaggtt cgcgacgtag
     4321 gcggcccggt cgtaggaccg gcccagcgcg tccgggggtg cgatctggtc cgcgatcacg
     4381 agggtgtcga cgtcgtccag ggccgctccg tcccgggcga tctcgaaggg agtgagcttc
     4441 gccatgccac ccttcacgta cttcgcggtg tcctcgaacc agtccatgtt cgaggcgctg
     4501 tacggtcgct gcgcgaccgg tcgaccgttc tgtccgacgc cgttgccgct cggtcccggc
     4561 agacgcccgg caccgttctc gtcatcgtgc cgaacccgga cgggatcgaa gacgtacccg
     4621 accttgccct tcgggtcgat ctggaagtcg gcgaactcct gctcctggaa catcgcgtag
     4681 gccatcgcgg tcttgatgat ggcgcggctc gcgttgatgt agttctcctg aagcacgacc
     4741 gaccagggct tcccggcggc acggatggac gtgtagctgt ccgcatggtt cagcgcgatc
     4801 tcgtagtcca tgcccgtcac gccgagctcg ttggccatgt agtcgccgat gaagccggtg
     4861 tcggtgtacc cgagggtgtc ccagacggtc ccccaccggg cgggcttcgt catgacggtg
     4921 ttcttcggga tcgagccgtg gtggtcctcg atgccttcgc cggcgtcgcc gccggttgct
     4981 tcctcaccga ggttcacgag gccgaggaag agcgtgtcgt ccatcaccga cttcgtccgc
     5041 tcggcgatcg ccatgagctg gcgatgcttg atggagtcga actgtccggc tggatacatg
     5101 atgtccgccc acgcccgcga ctgcgattcg ccgtggatgt cggcgccgta ggccatcagc
     5161 tcgcccttcc ccgcctccgc cacctcgtgc atgaagcggt ggccgaagtt catctcggac
     5221 tcgaagagcg ggttccgcga ggggttgatg tagccgacgg tcggcatctg ccggttcagg
     5281 tccgtgttca ggtagttgcc gcggctgtag aggccgggcc agttggcctg gtcgccacga
     5341 gcccagccgt ccatgttgaa gttgacgaag tagaccgcct gcttccgcag gatcgtgcgg
     5401 atcgggtacg agtggaagtc cgggtcgacg cccgtggtgg actcgtatcc cggaacgccg
     5461 tcggcgatgc gatcgcgggc ggggtcgttg cgctcggccc ccatcgccag atcctcgacc
     5521 gcccgcagac caccctcgat gccgcccttc tcgtcgccgt ggaccgagag cgagaagagc
     5581 atcgtgtgct tgccgtcgtc cggcacttgg tggtcggtga gcttgacgac ccagatgtcg
     5641 tacccgtctc cggtgtccgt cgggtcgtgc gaacgcttga ggtcgggacc cacactgacg
     5701 gcgtcgtcgt cgccgtagtg ctgccgcagg ttgaagacgg agacccaccg cgggtagagc
     5761 tgggcgagat acttgatccc ggcctcgaac tccttgaact gcgttcccgg ctgcggcagg
     5821 aactggacgt aatcacgcgc gatcgccgcc tcggggaaga cgcggccacc gcacgccgcg
     5881 cgcggcgggc cccccatgaa gccgtcggtg cagacgggcg tcgccgaggc gttcggcacg
     5941 cccagggcca ggccccccac caccgtcagc gagatcgtcg ccgctcgcag gaaccgtcgc
     6001 atcggtcgct ctccttcggt cggatggcgc tgtgttcgct acggcggccc ggaaaccctg
     6061 tgtgcagctg cacaaatcgg gcgaccgtga gcggcggtcg gagcgccggt gggaggagag
     6121 ctggcggtgg gattcgaacc cacgacctag cgattacaaa tcgcttgcgc taccggactg
     6181 cgccacgcca gcggaggcta gtgtaccggc gcggtgggcg ccgccggaga ggcagcgtcg
     6241 cgctcctcgg cacggttccg cttggcccgc gcccgcaggt ggaggtccac cttgcgcttg
     6301 atccgctgga gggcgttgtc gatcgacttg acgtgccgcc cgaggtagtc cgagatctcg
     6361 tggtacgtct tcccctcgac gtagaggcgg aggacctcgg actcgaactc cgagaggatc
     6421 tccgagaacg cgaccttgat gctcgcgagc tcctcgttgg agatgaccat gtccgcggga
     6481 tcggaggtct gcacgctctt cagcacgtcg acgaggaccc ggtccgcatc gccgtcagtg
     6541 gcgacaggct tggacaacga cacgtaggtg ttcagcggac cgtgcttctg ccgggtcgcg
     6601 gtcttgatcg cggtgatgat ctggcgggtg atgcagatct ccgcgaaagc ccggaacgcg
     6661 gactgcttcg aggcgctgaa gtcgcggatg gccttgtaca ggccgatcat gccttcctgg
     6721 acgatgtcgt cccggtcggc accgacgagg aagtaggagc gcgccttcat ccgggcgaag
     6781 ttgcggtagc ggctcagcag gacctcgatg gcccgctgat ccccgtgccg gtacgagagg
     6841 acgagctcct cgtcagagac ctcagtggcc tccaggtacg ggccagtcgc cgtgctcgcc
     6901 accgccatcc aggctcctcc ggatcttcgg atcatccatc gacgcgacgg cgggcacctc
     6961 gcgttcggcg cgcttccgat tatgcggcct cggctgcccg tggcgcaagg ggttgcgcag
     7021 gggagatact agcacgaatc gggcgttccg gtcacggatt tcctgcttcg aggactagtt
     7081 cttccgggcc atcggcccga ggacgtagag ggcgagcgcg gaagcgacgg ccacgttcag
     7141 cgagtccaca cggcccagca tcgggatcga agcaagctcg tcgcagcgct cccgaacgag
     7201 ccgcgagagg cccgggccct ccgcgcccgc gaccacggcg agccgcccat ccggtgggtc
     7261 gagctcgtcg atccggcggg gggcgtcggc gtccagcccg atgctccaga accccgcttc
     7321 ctggaggcgc tggatcgcgc gaccgatgtt cgcgacgcgc gccatgggca ggtgcagcag
     7381 cgcgccggcg gacgcgcgga ccgcggccgc gctcacggcg gccccccggt gacgccggct
     7441 cacgaggacg gatgcaccgg cggcctcggc gccccgcgcg gcggcaccga ggttctgggg
     7501 gtcggtgacg ccgtcgagga cgagcgccag cgccgatccc ggccactctc gcccggcgag
     7561 gtcggcctcc ccgagctccg ccgggagccg gacgcgcgcg accacgccct gatggggcgg
     7621 cccctgggcg aggcggtcga g
//
LOCUS       DATLGG010000295         5598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_234448, whole genome shotgun sequence.
ACCESSION   DATLGG010000295 DATLGG010000000
VERSION     DATLGG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234448"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VM638_08410"
     CDS             complement(<1..229)
                     /locus_tag="VM638_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM12481.1"
                     /translation="MSARVERRDQLRAGTPARRRRRWFLPVYSGVVIGYLFLPIAVMI
                     LYGFNDSRGRFLSFSWQGFTLRWYRDLFSIPE"
     gene            complement(226..1068)
                     /locus_tag="VM638_08415"
     CDS             complement(226..1068)
                     /locus_tag="VM638_08415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM12482.1"
                     /translation="MRKRRAPYLLLLPGALWLTAFFVVPIASMLSLSLQEGTVERGFE
                     LTWRWSTYADVLAKFDVQLVRSLTYGLIVTVLCLVVAYPLAYTIALRGGRWKNALLFL
                     VVLPFFVSLIIRTLSWKFILADQGFLLAPMKAIGLVPDGFRFLATPVAVIGGLFYNFL
                     PFMTLPLYVALERMDRSLLEAAGDLYSSRATAFWRVTFPLSLPGVFAGSLLTFIPSVG
                     DFINAELLGGTGTTMIGNVIQYEFLRANDYPEAAALSFILMAGILVGVALYTRVLGTE
                     ELSL"
     gene            complement(1091..2323)
                     /locus_tag="VM638_08420"
     CDS             complement(1091..2323)
                     /locus_tag="VM638_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HVM12483.1"
                     /translation="MTELERLFGRMTDPLTRRELLRRAGRGSAYLGMMAFLAACGVRG
                     QRQEAPPSPSPLGEPAGELNVANWPLYIDQDDDGNSQTIADFEEETSIKVSHREVIDD
                     NESFYGTIREPLAAGQSTGWDLIVVTDWLIARMIRNNYLEPLHHDRIPNFFSEAGAIY
                     QDRSYDPGNRFSVPWQSGITGIGYDPELTGREITSWNDLFDPAFKGKVGMFTEMRDTV
                     NLGLLGNGVDPQQATIEDVERVQQQLLDQRERGIVRGYYGNEYADALVTGDLAITMAW
                     SGDIFQKQFDKPRLRFVVPQEGGILWTDNMAIPAGAEHPADAHEFVNFVYRPEIAAQI
                     AEYVNYITPVPAAKDFILQHAEEAEDAEDAEYLRGVAESPLVFPTPEMEGRLHSYRVL
                     DEEEEEQWNELFQEVAQA"
     gene            complement(2316..3428)
                     /locus_tag="VM638_08425"
     CDS             complement(2316..3428)
                     /locus_tag="VM638_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12484.1"
                     /translation="MDQADVRLLDVMKRFGEVVAVDHVSLEIGHGEFFSLLGPSGCGK
                     TTTLRLIGGFEEPDAGAIELAGRDVAGLPPYRRDVNTVFQSYALFPHLDVFENVAYGL
                     RRKRVDGAEIRRRVGQMMDLVDLPGLERRRISQLSGGQQQRVALARALVNEPKVLLLD
                     EPLGALDLKLRRQMQLELKRIQHEVGITFVYVTHDQDEAMTMSDRLAVMNRGRVEQVG
                     PPQEVYERPATAFVAGFLGASNLLDGKVHGREGDLGTVVLASGEKVRVPVRFLAEADA
                     VRVGVRPEKIRLLPEGSPAPEGVNSIAGTVEVSTFAGVSNVYSVRGPAPEPIVVYAQN
                     SGAAAEGPAAAGSRVLLIWEPEHTFAVEAEEGGNGD"
     gene            complement(3567..4220)
                     /gene="sigH"
                     /locus_tag="VM638_08430"
     CDS             complement(3567..4220)
                     /gene="sigH"
                     /locus_tag="VM638_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HVM12485.1"
                     /translation="MSVTARDITRETPDEDLVAAYQAGEPGAMETLLERYRDFARLKA
                     RSYFLAGADRDDIVQEGMIGLYKAIRDYRADGDAAFRTFAEVCITRQILTAVKTASRH
                     KHGPLNTYVSLATPVSETEGEDRTLADVIPAPAMTDPAEMLLSHENVLAIRMAFSELL
                     SEFETEVLHLYVEGKSYEDIAGRLGRSAKAVDNALQRIKRKLELHTQDHEPERADLT"
     gene            4498..4722
                     /locus_tag="VM638_08435"
     CDS             4498..4722
                     /locus_tag="VM638_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017580863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HVM12486.1"
                     /translation="MGRRPELPRGDAWRGAGIAWTMTAELLAAILVWGLIGAGMDRLV
                     GTGKLFLAIGMVLGAATGIYLVYVKHGRDE"
     gene            4719..5168
                     /locus_tag="VM638_08440"
     CDS             4719..5168
                     /locus_tag="VM638_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12487.1"
                     /translation="MTRPEHAMVRRALRSGLLLAPVAVLLGSLAGAPGAISAAIGIAV
                     VLANFAAHGLSLAWAAGVSVSAVHAVALGGFVLRMGVIVGLLFALRPLDFFSPLAFGL
                     AVAGGTIALLVHEARIMASAPGAQLEIPPDPVAVAARDRLRAEERPA"
     gene            5165..>5598
                     /locus_tag="VM638_08445"
     CDS             5165..>5598
                     /locus_tag="VM638_08445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVM12488.1"
                     /translation="MSLLTLLAAPSRPPDPFKAPGLEIFEAPCLTEFDAFGVHFCVNR
                     VTIYMFLASLIVISLFVAAASRSDQLVPRGIRNFVEVVVEFVRNQIAIEVIGPEGRAW
                     VPFLTSMFTFLLVGNLFGVIPGIQYPINGRMAIPAFLAILTWV"
BASE COUNT          831 a         1952 c         1921 g          894 t
ORIGIN      
        1 gctcggggat cgagaacagg tcgcggtacc agcgaagcgt gaacccttgc caggagaacg
       61 agaggaaccg cccccggctg tcgttgaagc cgtagaggat catcacggcg atcgggagga
      121 agaggtagcc gatcaccacc ccggagtaga ccgggaggaa ccagcgccgc cgccgccgcg
      181 cgggagtgcc ggcccggagc tggtcacgac gctcgacccg ggcgctcaca gcgagagctc
      241 ctcggttccg agcacccggg tgtacagcgc gacgccgacg aggatccccg ccatcaggat
      301 gaacgacagg gccgccgcct ccgggtagtc gttcgctcgc aggaactcgt actggatcac
      361 gttcccgatc atcgtcgtcc ccgtcccgcc cagcagctcc gcgttgatga agtcgccgac
      421 cgacgggatg aacgtgagca gcgacccggc gaacacgcct gggagcgaca gcgggaaggt
      481 gacgcgccag aacgcggtgg cgcggctcga gtacaggtct ccggcggctt cgaggaggct
      541 ccggtccatg cgctcgaggg cgacgtagag gggcagggtc atgaacggga ggaagttgta
      601 gaacaggcca ccgatgacgg ccaccggcgt cgcgaggaag cggaacccgt caggcacgag
      661 cccgatcgcc ttcatcggcg cgaggaggaa cccctggtcg gccaggatga acttccacga
      721 cagcgtccgg atgatcagcg agacgaagaa cggcaggacc acgaggaaca ggagcgcgtt
      781 cttccagcgg ccgccgcgca gcgcgatcgt gtacgcgagc gggtaggcca cgacgaggca
      841 cagcaccgtg acgatcaggc cgtaggtcag cgaccgcacg agctggacgt cgaacttcgc
      901 gagcacgtcg gcgtacgtgc tccaacgcca ggtcagctcg aaccctcgct cgaccgtgcc
      961 ctcctggagc gagagcgaga gcatcgaggc gatgggcacc acgaagaacg cggtcagcca
     1021 cagggcgccc ggcagcagca gcaggtaggg cgcgcgccgc ttcctcatcg tcccccggcg
     1081 cccgccgacg ctacgcctgc gcgacctcct ggaacagctc gttccactgc tcctcctcct
     1141 cttcgtccag gacgcggtag gagtgcaggc gaccctccat ctccggcgtc gggaagacca
     1201 gggggctctc ggcgaccccg cgcaggtatt ccgcgtcctc agcgtcctcg gcctcctcgg
     1261 cgtgctggag gatgaagtcc ttcgccgcgg ggaccggagt gatgtagttc acgtactcgg
     1321 cgatctgcgc tgcgatctca ggccggtaga cgaagttcac gaactcgtgc gcgtccgccg
     1381 ggtgctcggc gccggcgggg atcgccatgt tgtccgtcca caggatcccg ccctcctggg
     1441 ggacgacgaa gcgcaggcgc ggcttgtcga actgcttctg gaagatgtcg ccggaccacg
     1501 ccatcgtgat cgccaggtcg ccggtgacga gcgcgtccgc gtactcgttc ccgtagtacc
     1561 cgcggacgat cccgcgctcc cgctggtcga ggagctgctg ctggacgcgc tccacgtctt
     1621 cgatcgtggc ctgctggggg tcgacaccgt tgccgagcag tccgaggttg accgtgtcgc
     1681 gcatctcggt gaacatgccg accttcccct tgaaggccgg gtcgaacagg tcgttccacg
     1741 acgtgatctc gcgcccggtg agctcggggt cgtagccgat cccggtgatc cccgactgcc
     1801 acggcacgct gaagcggttc cccgggtcgt aggagcggtc ctggtagatc gctccggcct
     1861 ccgagaagaa gttcgggatc cggtcgtggt ggagcggctc gaggtagttg ttgcggatca
     1921 tccgcgcgat cagccagtcc gtcacgacga tgaggtccca gcccgtggac tgaccggcgg
     1981 cgagcggctc gcggatcgtc ccgtagaacg actcgttgtc gtcgatgacc tcgcggtggg
     2041 agaccttgat gctcgtctcc tcctcgaagt cggcgatcgt ctggctgttg ccgtcgtcgt
     2101 cctggtcgat gtacagcggc cagttcgcga cgttgagctc acccgcgggc tcaccgagcg
     2161 gcgacggcga cggcggcgcc tcctggcgct ggccgcggac cccgcacgcc gcgaggaacg
     2221 ccatcatccc gaggtacgcc gatccgcgac ccgcccgacg cagcagctcc cgccgggtca
     2281 gcggatccgt catccggccg aacaggcgct cgagttcagt caccgtttcc cccttcctcc
     2341 gcctccacgg cgaacgtatg ctccggttcc cagatcaaca ggacgcggct gccggcggcc
     2401 gccggacctt ccgccgccgc gccgctgttc tgcgcgtaga ccacgatcgg ctcgggcgca
     2461 gggccccgca ccgagtacac gttgctgacg ccggcgaacg tggagacctc cacggtgccc
     2521 gcgatgctgt tgaccccctc cggcgccggc gagccttcgg ggaggagacg gatcttctcc
     2581 ggccggacgc ccacgcgcac ggcgtcggcc tcggcgagga agcggacggg cacccgcacc
     2641 ttctcgcccg aggcgagcac cacggtgccg aggtcgccct cccgcccgtg gaccttcccg
     2701 tccagcaggt tcgacgcccc gaggaagccc gcgacgaacg ccgtggcggg ccgctcgtac
     2761 acctcctgcg ggggaccgac ctgctcgacc cgcccacggt tcatcaccgc gaggcggtcg
     2821 gacatcgtca tggcttcgtc ctggtcgtgc gtgacgtaga cgaaggtgat cccgacctcg
     2881 tgctggatcc gcttgagctc cagttgcatc tggcgccgca gcttgagatc gagcgcgccg
     2941 agcggctcgt cgaggagcag gaccttgggc tcgttcacaa gggcgcgcgc gagcgccacc
     3001 cgctgctgct ggccgcccga gagctgcgag atgcggcgcc gctcgagtcc ggggaggtcg
     3061 acgaggtcca tcatctggcc gacccgccgc cggatctcgg ctccgtcgac gcgcttgcgc
     3121 cggagcccgt aggcgacgtt ctcgaagacg tcgaggtggg ggaagagcgc gtaggactgg
     3181 aagacggtgt tgacgtctcg ccggtacggc ggcaaccccg cgacgtcgcg gcccgcgagc
     3241 tcgatcgctc ccgcatcggg ctcctcgaac ccgccgatca gccggagcgt cgtggtcttg
     3301 ccgcacccgg agggcccgag gaggctgaag aactcgccgt gcccgatctc gagcgacacg
     3361 tggtcgacgg cgacgacctc gccgaaccgc ttcatgacgt ccaggagccg gacgtcggcc
     3421 tgatccacgc gcgcctccac gatcgtcccg cccgacccta tcgggccgtc cgcggcagga
     3481 ccgcgaacgg cagggcggaa aggtcctggg ggctttgtac atcccggcgg gactccggcg
     3541 ccaccgtcct ggggccgggc cgcgggtcag gtgaggtccg cgcgctccgg ctcgtggtcc
     3601 tgggtgtgga gctcgagctt ccgcttgatc cgctggaggg cgttgtcgac ggccttcgcg
     3661 ctgcgcccga ggcgacccgc gatgtcctcg taggacttcc cctcgacgta gaggtggagc
     3721 acctcggtct cgaactccga gaggagctcg gagaaagcca tccggatcgc gaggacgttc
     3781 tcgtgcgaga ggagcatctc ggcggggtcg gtcatcgccg gcgccgggat gacgtccgcc
     3841 agcgtccggt cctcgccctc ggtctcgctg acgggagtgg cgaggctcac gtaggtgttc
     3901 agcgggccgt gcttgtggcg gctcgcggtc ttgacggcgg tcaggatctg ccgggtgatg
     3961 cagacctccg cgaaggtccg gaacgccgcg tcgccgtcgg cgcggtagtc gcggatcgcc
     4021 ttgtagaggc cgatcatgcc ctcctggacg atgtcgtcgc ggtcggcccc cgcgaggaag
     4081 taggagcggg ccttcagccg cgcgaagtcc cggtagcgct cgaggagggt ctccatcgct
     4141 cccggttcgc cggcctggta ggcggcgacc agatcctcgt ccggcgtctc cctcgtgatg
     4201 tcccgtgccg tgacgctcat gcgacccgcc tctcgtcgtt gcaccccgtc ttgcgctaat
     4261 cctcacggaa cggacaaagg atgcccatag ccacaaggtg gggtcttcgg gtagtccatc
     4321 cggaccatcc cccatctagc ccatcggccg ttcggcacgg cacctgaagg gcggccaact
     4381 cgtgaaaatt ttcacaagcg gccggtcgag agtcgcgaaa accctcatct acctgcggaa
     4441 acgcgttctc cctctttttg acacctcgga agagccgttg atagaatcgc gcggctcatg
     4501 ggtcgccgtc ccgaacttcc ccggggagac gcgtggcgcg gcgccgggat cgcctggacc
     4561 atgacggcgg agctgctcgc cgcgatcctc gtctgggggc tcatcggagc cgggatggac
     4621 cgcctcgtgg gcacggggaa gctgttcctg gcgatcggga tggtcctggg ggcggcgacg
     4681 ggcatctacc tggtctacgt gaagcacggg agagacgagt gacacgacct gagcacgcga
     4741 tggtgcggcg agcgctgcgg agcggcctgc tcctggcgcc ggttgccgtg ctgctcggca
     4801 gcctcgccgg ggcgccgggc gcgatctcgg ccgcgatcgg catcgccgtc gtcctcgcga
     4861 acttcgccgc ccacgggctg tccctcgcgt gggctgccgg cgtctccgtc tcggccgtcc
     4921 acgcggtggc cctcggcgga ttcgtcctgc gcatgggcgt gatcgtcggg ctgctgttcg
     4981 ccctccgccc gctggacttc ttctcgcccc tcgccttcgg gctcgccgtg gcgggcggca
     5041 cgatcgcgct cctcgtccac gaggcgcgga tcatggccag cgcgcccggc gcccagctcg
     5101 agatcccgcc ggacccggtc gcggtcgccg cgagggatcg ccttcgcgcc gaggagcggc
     5161 cggcatgagc ctcctgacgc tcctcgccgc accgagccgt ccgccggacc ccttcaaggc
     5221 tcccggcctc gagatcttcg aggcgccctg cctcaccgag ttcgacgcgt tcggcgtcca
     5281 cttctgcgtg aaccgcgtga cgatctacat gttcctcgcg tcgctgatcg tgatctcgct
     5341 gttcgtcgcc gcggcgagcc gcagcgacca gctcgtcccg cgcgggatcc gcaacttcgt
     5401 cgaggtggtc gtggagttcg tccgcaacca gatcgcgatc gaggtcatcg gtcccgaggg
     5461 acgggcgtgg gtccccttcc tcacctcgat gttcacgttc ctgctcgtcg gcaacctgtt
     5521 cggcgtgatc cccgggatcc agtacccgat caacggacgg atggcgatcc ccgccttcct
     5581 cgcgatcctg acctgggt
//
LOCUS       DATLGG010000296         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_185090, whole genome shotgun sequence.
ACCESSION   DATLGG010000296 DATLGG010000000
VERSION     DATLGG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185090"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VM638_08450"
     CDS             <1..641
                     /locus_tag="VM638_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HVM12489.1"
                     /translation="FFQKDLGSSLLFFGLVVTMLWVATARSAYLLIGGTMFAAGAFIA
                     YQVFGHVQDRITIWLHALDPRYIQDEGFQVAQSLFAMATGGITGTGLGEGRPQDIPDA
                     PTDFVFAAIGEELGLLGTMAVLLLFVLLIARGYRTALTRADGFGQLLATGLATILALQ
                     TFVIVGGVTRLIPLTGITLPFVSYGGSSLVANFVLLALLVRTSAGPSKDEAA"
     gene            641..2101
                     /locus_tag="VM638_08455"
     CDS             641..2101
                     /locus_tag="VM638_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVM12490.1"
                     /translation="MERQIRRLATGALVLFGLLAVNVNYIQVVAAERLANHPANKRLL
                     IQEYDVQRGQILAADRRTVLARSRATKGDLKYLRRYPDGQRYAHITGYYSFVFGRSRL
                     EQGFNDVLSGRSAELLPQRFIDEILGRDQRGGTLVLSIDPRVQEAAESGLAGRRGAVA
                     AVNPQTGEVLALVSHPTYNPNRLSSHDGKQIRRAWEQLNADPQKPLRSGATDEFFAPG
                     STFKVITAAAALEDGMSPEDTLPNPPALDLPQTDRQLHNFGGGQCPGGSRITLAQALQ
                     VSCNVSFGQLGLDLGGEKLEEQARRFGFMEEIPFDIAFQEGQFPDPAGFAQNQPGLAF
                     SAIGQQDIRANVLHMALVAGAIGNRGTMMAPKLVTEIRDPSGRVLRRLEPQVWGEPMS
                     GRNARDLTAMMQAVVEAGTATAAQIPGVAVAGKTGTAQDPGSDPHAWFISFAPAERPT
                     VAVAVVVLGGGDLGSEATGGALAAPIARQVMEAALR"
     gene            2101..>2807
                     /locus_tag="VM638_08460"
     CDS             2101..>2807
                     /locus_tag="VM638_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVM12491.1"
                     /translation="MAGRILGDRYELGPVVGRGGMATVYRARDRVLHREVAVKVLQPQ
                     LARDQEFIERFRREARAAAGLSHPNIVGVHDHGSDGSDHFIVMEFVEGRTLAEVLDEE
                     GALETGRAIAIATDVAAALSAAHERGLVHRDVKPANILLTDRGRVMVTDFGIARAATS
                     QGLTMTGTVLGTASYLSPEQARGEPVDERSDVYSLGCVLYEMLTGEPPFAADSPVAVA
                     ALHVNADPPRPSAHQPGV"
BASE COUNT          396 a         1016 c          991 g          404 t
ORIGIN      
        1 tgttcttcca gaaggacctc gggtcctcgc tgctgttctt cggcctcgtc gtgacgatgc
       61 tgtgggtggc aacggcgcgc agcgcctacc tcctgatcgg ggggacgatg ttcgcggccg
      121 gcgcgttcat cgcgtaccag gtgttcgggc acgtgcagga tcggatcacg atctggctgc
      181 acgcgctcga cccgcgctac atccaggacg aagggttcca ggtcgcgcag tcgctgttcg
      241 cgatggcgac cggagggatc accggcaccg ggctcgggga aggccggccc caggacatcc
      301 ccgacgctcc gacggacttc gtcttcgccg cgatcgggga ggagctcggg ctgctcggaa
      361 cgatggcggt cctgctcctg ttcgtcctgc tgatcgcgcg cggctaccgg acggcgctca
      421 cgcgggccga cgggttcggc cagctgctcg ccacggggct cgcgacgatc ctcgcgctcc
      481 agaccttcgt gatcgtcggc ggggtgacgc gcctgatccc cctcaccggg atcaccctgc
      541 cgttcgtctc ctacggggga tcgagcctcg tggcgaactt cgtgctcctc gccctgctcg
      601 tgcgcacctc ggcgggccca tccaaggacg aggccgcctg atggagcggc agatccgccg
      661 tctggcgacc ggggcgctcg tgctcttcgg cctcctggcg gtcaacgtca actacatcca
      721 ggtcgtcgcg gccgagcggc tggcgaacca tcccgcgaac aagcggctcc tgatccagga
      781 gtacgacgtc cagcgaggcc agatcctcgc ggcggaccgc cggaccgtcc tcgcgcggag
      841 ccgggcgacg aagggcgacc tgaagtacct ccgtcggtac cccgacgggc agcgctacgc
      901 ccacatcacc gggtactact cgttcgtctt cggccggagc cggctcgagc aggggttcaa
      961 cgacgtcctc tccgggcgct cggcggagct gctgccacag cggttcatcg acgagatcct
     1021 cggccgcgac cagcgcggcg ggacgctcgt cctgtcgatc gatccccgcg tccaggaggc
     1081 cgccgagagc gggctcgccg ggcgtcgggg ggcggtcgcg gcggtgaacc cgcagaccgg
     1141 cgaggtcctc gcactcgtct cccaccccac gtacaacccg aaccgcctgt cctcgcacga
     1201 cgggaagcag atccgccgcg cgtgggaaca gctgaacgcc gacccgcaga agccgctccg
     1261 ctcgggggcg accgacgagt tcttcgcccc cggctccacc ttcaaggtca tcacggcggc
     1321 ggccgccctc gaagacggga tgtcgccgga ggacacgctc ccgaacccgc cggcgctgga
     1381 tcttccccag accgaccgtc agctccacaa cttcggcggc ggccagtgcc ccggcggctc
     1441 gcggatcacg ctcgcgcagg cgttgcaggt ctcgtgcaac gtctcgttcg ggcagctcgg
     1501 gctcgacctc ggcggggaga agctcgagga gcaggcgcgt cgcttcgggt tcatggagga
     1561 gatcccgttc gacatcgcgt tccaggaggg gcagttcccc gaccctgccg gattcgcgca
     1621 gaaccaaccc ggcctcgcgt tctcggcgat cggacagcag gacatccgcg cgaacgtgtt
     1681 gcacatggct ctcgtcgccg gcgcgatcgg caaccgcggg acgatgatgg cgccgaagct
     1741 cgtcacggag atccgcgacc catccggcag ggtcctgcgc cgcctcgagc cgcaggtctg
     1801 gggcgagccg atgagcgggc ggaacgcgcg cgacctcacc gcgatgatgc aggcggtggt
     1861 cgaggccggg accgcgacgg cggcgcagat ccccggggtg gcggtagccg gcaagaccgg
     1921 caccgcgcag gacccgggaa gcgacccgca cgcctggttc atctccttcg cgccggcgga
     1981 gcgacccacg gtggccgtcg cggtcgtggt cctcggtgga ggggacctcg gcagcgaggc
     2041 gaccggcggc gcgctcgcgg ctccgatcgc tcgccaggtg atggaagcgg cgctccgctg
     2101 atggcgggac ggatcctcgg ggaccgctac gagctcggcc cggtcgtcgg ccggggcggg
     2161 atggcgaccg tctaccgggc gcgcgaccgg gtcctgcacc gcgaggtggc cgtcaaggtc
     2221 ctccagccgc agctcgcccg cgaccaggag ttcatcgagc ggttccgtcg cgaggcccgc
     2281 gccgccgcag ggctgagcca cccgaacatc gtcggggtgc acgaccacgg ctccgacggc
     2341 tcggaccact tcatcgtgat ggagttcgtc gaaggccgga cgctcgcgga ggtgctggac
     2401 gaggaggggg cgctcgagac gggtcgtgcc atcgcgatcg ccaccgacgt cgccgccgcc
     2461 ctctcggccg cccacgaacg ggggctggtc caccgcgacg tcaagcccgc gaacatcctg
     2521 ctgaccgacc gcgggcgcgt catggtgacg gacttcggga tcgcccgagc cgcgacgtcg
     2581 caggggctca cgatgaccgg gaccgttctg gggaccgcgt cgtacctctc gccggagcag
     2641 gcccggggcg aaccggtcga cgagcgatcc gacgtctact ccctcggctg cgtgctgtac
     2701 gagatgctca ccggcgagcc gcccttcgcc gccgattcgc cggtcgccgt ggcggccctc
     2761 cacgtgaacg ccgatcctcc ccggccgtcg gcccaccagc ccggggt
//
LOCUS       DATLGG010000297         6128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_106081, whole genome shotgun sequence.
ACCESSION   DATLGG010000297 DATLGG010000000
VERSION     DATLGG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106081"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VM638_08465"
     CDS             complement(<1..863)
                     /locus_tag="VM638_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HVM12492.1"
                     /translation="MTLPFIDGEELSRSLPISDAVEALAAAFASEERPEAPLRGHVEL
                     DGGDLLLMPAHGAPGLGVKLVTLNPGNPGRGLPLIHAVYVLFSPATLAPVAAIDGAAL
                     TALRTSAVSALATRHLARADASRLVLFGAGVQARAHLVAMRAVRPIRWVRVISRSPDR
                     ARRLADEAAAAGLDAAVAGTDAVAEADIVCTCTTSTTPVLTGRNLRAGAHVNAVGAYR
                     PDAREVDGETVLRAALVAVETREAALAEAGDLVIPIERGDLDPATLVELGAVVRGERG
                     RARPLPARRASP"
     gene            complement(860..1594)
                     /locus_tag="VM638_08470"
     CDS             complement(860..1594)
                     /locus_tag="VM638_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12493.1"
                     /translation="MTERNGNVILELRDVSTSYGPVRVLEDVNLEIRKGEIVCLLGSN
                     AAGKTTTLRTILGLTHPWKGEIIFEGQRIDGLVTTQVVSRGLTMVPENRRLFGRMTVR
                     ENLEIGANLRRDKTEIQGDLERILGLFPRLRERITQRAGTLSGGEQQMLAIGRAMMAR
                     PHVLLMDEPSMGLAPILVEQVFETIAEINRQGTTIFLVEQNANMALSVAHRGYAIQTG
                     RIVLADTAENLKNNPQMQEAYLGELE"
     gene            complement(1591..2364)
                     /locus_tag="VM638_08475"
     CDS             complement(1591..2364)
                     /locus_tag="VM638_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12494.1"
                     /translation="MSLLEVTGLARRFGGVRAVDGVDFHAEAGEIVGVIGPNGAGKTT
                     LFNIVTGFYEPDSGEIIFDGHNLVGLKPNQITKLGIARTFQSVRLFPNMTVLENAMVG
                     QHARTRSGVTGAVFGLPHTKREEEEIKRKAREMLSFFGDVLTGFREEQLAAELAYADR
                     RRLEIARAMATEPKLLLLDEPAAGMNPQEKKDLIKLIGRLRDERGYAVVLIEHDMAVV
                     KGISDRVVALDYGQKIAEGTYEEVSSNQRVIEAYLGKQK"
     gene            complement(2361..4412)
                     /locus_tag="VM638_08480"
     CDS             complement(2361..4412)
                     /locus_tag="VM638_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12495.1"
                     /translation="MGEVTGTFDVPESEARVTSPTRRLVNIGLIGGVVLIYLALTGIV
                     ERFHTRNVITGATGEGLLQLSRTMLIVALLLTGYLAGRGRKGEPRRGSPVLRGLAAGA
                     ISGALLALTVVVLKTLLASGINPREVLIAVSGTLLDTILFGEQGTAVAALIIVGASAL
                     IAGLGAAFVLLSRRTRRPVVTALATTLLVSMMEPLLRPIFVGLELPSEWLYSRGGLTV
                     TGAIIVLVLTLGIALALAWRQPEDRQGAAVAPPRGRAQEVSQLDRVTMWLGVSGFVLA
                     VVIVVMNFANAEVIPLFLLFLLLLNVAGLILSVAAGRRARAEGTRPSSQIALAGAAAA
                     AGIFLILPPIAGPFISDVLGTVGLYILLGLGLNIVVGYAGLLDLGYVAFFAVGAYAMA
                     IFTSRASFLVVETGGGVQNLNYAEQGWTTNFWFALPLVVIIAVISGVLIGAPVLRLRG
                     DYLAIVTLGFGEIIRTLVLSDWLAKWLGGAQGIIQIPAIRPPEALNLRDPERIYYVIF
                     IFALVAAWISFRLVESRVGRAWAAMREDESVAEAMGISVIKYKLLAFAMGAGIGCLGG
                     TFFAAKIGSIFPNSFHLLVSINVLAVIVLGGMGSIPGVVVGALVLVGLPELLREFAEY
                     RLLLYGAILVAIMIYKPEGLIPNRRRLQELHVQESEEDQYERGGPAPEPVIVTAEKEE
                     G"
     gene            complement(4416..5534)
                     /locus_tag="VM638_08485"
     CDS             complement(4416..5534)
                     /locus_tag="VM638_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVM12496.1"
                     /translation="MANETTNQTKPTPERPRPRRRLGFLPERIPVRGTILVILSILIV
                     LGVVLGTMSTLRTGRYGPQDWRNFVISGVARGSVYALIAMGYTLVYGILFMINFAHGE
                     VFMSGAFTSFFVARAMAQAGFLDSNPILAIIILLLVSATVSTLVAVTLERVAYRPLRG
                     APRLVPLITAIGASLFLQYTFRGLYGAQAKGYPTPGILQGRWNIFGMPIARTQVVVIV
                     GALLLMVGLYLFISRTRTGRSMRAVGEDKEIAALMGINVDRTIVTTFAIGGMLAGAAG
                     ILYVFLFPQVIFDMGFVPGIKAFTAAVLGGIGSVTGAGVGGLILGILEAVGPSLFLTG
                     ANVPAPHQLQPVVAFGILVLVLIFRPGGIFGSAEEKRA"
     gene            complement(5618..>6128)
                     /locus_tag="VM638_08490"
     CDS             complement(5618..>6128)
                     /locus_tag="VM638_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM12497.1"
                     /translation="DRGGLLTQQARETSGLENTDLGGSDGMFTPDWIEAAGDAAEGVY
                     ISGPDLSAFAGGDFYKNEFLPAYEEKFGEKPQSVFHAHAFDATNMILDAIEQVAIEGE
                     DGGLQIPRQELRDALFNTQGFEGITGTLSCDENGDCQQIAVMAIHQVKDGDFGDAIFS
                     KEYNLAELG"
BASE COUNT          978 a         2146 c         2110 g          894 t
ORIGIN      
        1 ggcgacgctc gtcgagctgg gagcggtcgt gcgcggcccc gctccccgcg cacgaccgct
       61 cccagctcga cgagcgtcgc cgggtcgagg tccccgcgct cgatcgggat gacgaggtcc
      121 cccgcctccg ccagcgccgc ctcccgcgtc tccacggcca cgagggcggc gcgcaggacc
      181 gtctcgccgt cgacctcgcg cgcgtccgga cgataggcgc cgacggcgtt cacgtgagcg
      241 cccgcgcgca ggttccgccc ggtgaggacg ggggtcgtgc tcgtcgtgca ggtgcagacg
      301 atgtccgcct ccgcgaccgc gtcggtgccg gcgaccgcgg catcgaggcc cgccgccgcc
      361 gcctcgtccg ccagccggcg cgcccgatcc ggcgagcgag agatcacgcg gacccagcgg
      421 atcggccgaa ccgcgcgcat ggccacgagg tgcgcccgcg cctgcacgcc cgccccgaac
      481 aggaccagcc tcgaggcgtc cgcccgtgcc aggtgccgcg tggcgagcgc ggagacggcc
      541 gacgttcgca gggcggtcag cgccgcgccg tcgatggccg ccacgggcgc cagcgtcgcc
      601 ggcgagaaca acacgtacac cgcgtggatc agcgggagcc cgcggccggg gttgccgggg
      661 ttcagcgtca cgagcttgac cccgaggccc ggcgccccgt gcgccggcat gagcaacagg
      721 tcgccgccgt cgagctcgac gtgcccccgc aggggcgcct cgggacgctc ctcggaagcg
      781 aaggcagcgg ccagggcctc gaccgcgtcg gagatcggaa gcgagcggga gagctcctcc
      841 ccgtcgatga acgggagggt cattccagct cgcccaggta tgcctcctgc atctgggggt
      901 tgttcttgag gttctccgcc gtgtcggcga ggacgatgcg ccccgtctgg atcgcgtagc
      961 cgcggtgggc gacggacagc gccatgttgg cgttctgctc gacgaggaag atcgtcgtcc
     1021 cctggcggtt gatctcggcg atcgtctcga agacctgctc gacgaggatc ggcgcgagcc
     1081 ccatcgaggg ctcatccatc aggagcacgt gcggccgcgc catcatcgca cggccgatcg
     1141 cgagcatctg ctgctcgccg ccggagagcg ttcccgcccg ctgggtgatc cgctcgcgca
     1201 gacgagggaa gaggccgagg atccgctcca ggtcgccctg gatctcggtc ttgtcccggc
     1261 gcaggttcgc cccgatctcg aggttctcgc ggaccgtcat ccgaccgaag aggcgacggt
     1321 tctccggcac catcgtcagc ccgcgcgaga cgacctgcgt cgtgacgaga ccgtcgatcc
     1381 gctggccctc gaagatgatc tcgcccttcc aggggtgcgt caggccaagg atcgtccgca
     1441 gcgtggtcgt cttgcccgcg gcgttcgagc cgagcagaca gacgatctcg cccttgcgga
     1501 tctccaggtt cacgtcctcg aggacgcgga cgggcccgta ggacgtgctg acgtcgcgga
     1561 gctccaggat gacgttgccg ttgcgctcgg tcacttctgc ttccccaggt aggcttcgat
     1621 gactcgctgg ttgctcgaca cttcctcgta ggtgccttcg gcgatcttct gcccgtagtc
     1681 gagggcgacg acgcggtcgg agatcccctt gacgaccgcc atgtcgtgct cgatcaggac
     1741 gacggcgtag ccgcgctcgt cgcgcaggcg cccgatcagc ttgatcaggt ccttcttctc
     1801 ctgcgggttc atccccgccg ccggctcgtc gaggagcagg agcttcggct cggtcgccat
     1861 cgcccgggcg atctcgaggc gtcgccggtc ggcgtaggcg agctcggccg cgagctgctc
     1921 ttcccggaag cccgtcagca cgtcgccgaa gaaggacagc atctcgcgtg ccttccgctt
     1981 gatctcctcc tcctcccgct tcgtgtgcgg gagaccgaag acggcgcccg tcacgccgga
     2041 ccgggtccgc gcgtgctgcc cgaccatcgc gttctccagg acggtcatgt tcgggaagag
     2101 ccgcacggac tggaaggtgc gcgcgatccc gagcttcgtg atctggttcg gcttgaggcc
     2161 cacgaggttg tgcccgtcga agatgatctc accgctgtcg ggctcgtaga agccggtcac
     2221 gatgttgaac agcgtcgtct tgcccgcgcc gttcgggccg atcacgccga cgatctctcc
     2281 ggcctcggcg tggaagtcca cgccgtcgac cgcccggacg ccgccgaagc gtcgcgccag
     2341 ccccgtgacc tcgagcaggc tcacccttcc tccttctccg ccgtcacgat caccggttcc
     2401 ggcgcgggcc cgccgcgctc gtactgatcc tcctcgctct cctggacgtg cagctcctgg
     2461 agacgccgcc ggttggggat gagcccctcc ggcttgtaga tcatgatcgc cacgaggatg
     2521 gcgccgtaga gcaggagccg gtactccgcg aactcacgca ggagctccgg cagcccgacg
     2581 agcacgagcg ctcccacgac gaccccgggg atgctcccca tgccgcccaa gacgatcacc
     2641 gccaggacgt tgatcgagac gagcaggtgg aagctgttcg ggaagatcga cccgatcttc
     2701 gccgcgaaga acgtcccgcc gaggcagccg atgccggcgc ccatcgcgaa cgcgaggagc
     2761 ttgtacttga tgacgctgat gcccatcgcc tcggcgaccg actcgtcctc gcgcatcgcg
     2821 gcccaggcgc ggcccacgcg cgattcgacc aatcggaagg agatccaggc ggccaccagg
     2881 gcgaagatga agatcacgta gtagatccgc tccggatctc gcaggttgag cgcctcaggc
     2941 gggcggatcg ccgggatctg gatgatcccc tgcgccccgc cgagccactt cgcgagccag
     3001 tccgacagga cgagggtgcg gatgatctct ccgaacccga gcgtgacgat cgcgaggtag
     3061 tcgccgcgca gccgcaggac cggcgctccg atgaggacgc cggagatcac cgcgatgatc
     3121 acgaccagcg ggagggcgaa ccagaagttc gtcgtccagc cctgctccgc gtaattgagg
     3181 ttctgaacgc cgccgccggt ctcgacgacg aggaacgagg cgcgagaggt gaagatcgcc
     3241 atcgcgtagg cgcccaccgc gaagaaggcg acgtagccga ggtcgaggag tcccgcatag
     3301 ccgacgacga tgttcagccc cagacccagc aggatgtata gccccaccgt cccgaggacg
     3361 tcgctgatga acggccccgc gatcggcggg aggatcagga agatccccgc cgccgccgcc
     3421 gcaccggcga gggcgatctg ggatgacggc cgtgttccct cggccctcgc gcgccggccg
     3481 gccgcgacgg acaggatcag tcccgcgacg ttcagcagga ggaggaacag gaggaacagg
     3541 gggatgacct cggcgttcgc gaagttcatg acgacgatca cgaccgcgag gacgaacccc
     3601 gagacgccga gccacatcgt cacccggtcc agctgggaga cctcctgcgc cctgccacgc
     3661 gggggagcca ccgccgcgcc ttgccggtcc tcgggttgac gccacgccag cgcgagggcg
     3721 atcccgaggg tcaggacgag gacgatgatc gctccggtga ccgtgaggcc tccccgcgaa
     3781 tagagccact cgctcggcag ctcgagcccg acgaagatcg gccgcagcag cggctccatc
     3841 atgctgacga gcagcgtggt cgcgagcgcg gtcaccaccg ggcgccgcgt ccggcgtgag
     3901 aggaggacga aggcggctcc gagcccggcg atcagcgcgg aagccccgac gatgatgagc
     3961 gcggcgacgg ccgtgccctg ctcgccgaac aggatcgtgt ccaggagcgt gcccgagacc
     4021 gcgatcagga cctcgcgggg attgatcccc gacgccagga gggtcttgag cacgacgacc
     4081 gtgagcgcga ggagcgcgcc ggagatcgcc cccgccgcca gcccccgaag cacgggggag
     4141 ccgcgtcggg gctcaccctt gcggccgcga cccgcgaggt acccggtcag gaggagcgca
     4201 acgatgagca tcgtccggct gagctgcagc agcccctcgc ccgtcgcgcc cgtgatgacg
     4261 ttgcgcgtgt ggaagcgctc gacgatgccc gtcagggcga ggtagatgag caccaccccg
     4321 ccgatcagcc cgatgttgac gagccgccgc gtcggcgacg tgacccgcgc ttcggactcg
     4381 ggaacgtcga aggtgccggt gacctcaccc atcgcctacg ccctcttctc ctcggccgac
     4441 ccgaagatgc ctccgggccg gaagatcagc acgagcacca ggatcccgaa cgccacgacc
     4501 ggctggagct ggtgcggggc ggggacgttc gcccccgtga ggaagagcga cgggccgacg
     4561 gcctcgagga tgccgaggat caggccgccg acgcccgctc cggtcacgct cccgatgccc
     4621 ccgaggacgg cggcggtgaa cgccttgatc cccgggacga accccatgtc gaagatgacc
     4681 tgcgggaaca ggaagacgta caggatcccg gccgcgccgg cgagcatccc gccgatcgcg
     4741 aacgtcgtga cgatcgtccg gtcgacgttg atgcccatga gcgccgcgat ctccttgtcc
     4801 tcaccgaccg cgcgcatcga gcggccggtc cgggtgcgcg agatgaagag atagagcccg
     4861 accatgagca gaagggctcc gacgatgacg accacctgcg tccgagcgat cggcatcccg
     4921 aagatgttcc agcggccctg aaggatcccg ggcgtcgggt accccttcgc ctgtgcgccg
     4981 tagagcccgc ggaaggtgta ttggaggaac agggacgcac cgatcgcggt gatgagcgga
     5041 accagccgcg gcgcccctcg gagcggacgg taggcgacgc gttcgagcgt cacggctacc
     5101 agcgtcgaga cggtggccga gacgagcagc aggatgatga tcgcgaggat cgggttcgag
     5161 tcgaggaagc cggcctgcgc catcgcccgt gcgacgaaga acgaggtgaa cgcacccgac
     5221 atgaagacct cgccgtgggc gaagttgatc atgaacagga tgccgtagac cagcgtgtat
     5281 cccatggcga tgagcgcgta gacgctcccg cgggccacgc cggagatcac gaagttgcgc
     5341 cagtcctggg ggccgtagcg cccggtgcgg agcgtgctca tcgtcccgag caccaccccc
     5401 agcacgatga ggatgctcag gatgacgagg atcgtgcccc ggacagggat gcgctccggc
     5461 aggaacccga ggcgtcgcct cggtcgcggc cgctccggcg ttggcttggt ctggttggtc
     5521 gtctcgttgg ccatcgttcg ttccggtagg ggcagggctg cgggtgggcc gtttccggcc
     5581 cacccgcagc ggacctaccc gacgtgcagg gtcgtgctta gccgagctcc gccaggttgt
     5641 actccttgga gaagatcgcg tcgccgaaat ctccgtcctt cacctggtgg atcgccatga
     5701 ccgcgatctg ctggcagtcg ccgttctcgt cgcagctcag ggttccggtg atgccctcga
     5761 acccctgcgt gttgaacagc gcgtcccgca gctcctggcg cgggatctgc aggccgccgt
     5821 cctcgccctc gatcgcgacc tgctcgatcg cgtcgaggat catgttcgtg gcgtcgaacg
     5881 cgtgggcgtg gaacaccgac tgcggcttct cgccgaactt ctcctcgtac gcggggagga
     5941 actcgttctt gtagaagtcg ccacccgcga aggcgctgag gtccggtccg gagatgtaga
     6001 cgccctcggc cgcgtcaccg gccgcctcga tccagtccgg ggtgaacatg ccgtccgacc
     6061 ccccgaggtc ggtgttctcc aggcccgagg tctcgcgcgc ctgctgggtc aggagcccac
     6121 ccctgtct
//
LOCUS       DATLGG010000298         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_217122, whole genome shotgun sequence.
ACCESSION   DATLGG010000298 DATLGG010000000
VERSION     DATLGG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217122"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VM638_08495"
     CDS             <1..614
                     /locus_tag="VM638_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12498.1"
                     /translation="PASAGLFIDPRDPALGVPLLYATTGRTGLDVFDLSRPHEPVRLG
                     SWSGAGLADVEVVASPAGRTVYAATEYWFGSTTAPRIVELDAGDLGAIVQRRRFSPGD
                     PPYPGGIPWRVQGLEAAGDYLYVAHSHAGLGILHTCCFTDPPRGSTTDLGEGNTGGEL
                     GSLSPYAMDVELLGETILVSDASTGVLSAFDFDPIWRHPEDEL"
     gene            722..2164
                     /locus_tag="VM638_08500"
     CDS             722..2164
                     /locus_tag="VM638_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12499.1"
                     /translation="MRRGLRGLILLSLLLGLLPATSIDAQGVECPTVEPPKLEFDTPT
                     YIDRDRAGGEPVSIVAEDGSISVSAHAGTTHIYKNPEAAGGAGDFLVGYTNQTLNWRS
                     VDGGKTWRYIGLAGLREGPHTVTSTGFSDPDFAMDMGGRIYNTEIDLANVAVFSSVDD
                     GQSYPFGNPEAASGDRPWLIGGEPDEVYLYVNLPKTFWRSTNGGLTFSLLPTPPINAK
                     GYRDPRNERNGLIGPVGTNGIAISSNKGQSWTTYNGANLGPNVDFFGSLAVDKAGHAY
                     KMAAGGYSGSTDTTPNGIVTYNYFDRDTNRWGQRVTIPTPPGDALWPWLVAGDDGRVA
                     LTWYQSLAGKPREFYAFVAYTHNARGTTVTCEDGSTRFIPPQFTVVNASQRPIHVGAI
                     CLDGTACNWNHGTNWDNGDRRLGDFFTINHDHEGNLFVVTTDTMLKSPTGGPKPVGNP
                     LFIKQKTGDRILAQPAPTRKTRCLWPLPSC"
BASE COUNT          365 a          850 c          736 g          310 t
ORIGIN      
        1 gtccggcgtc ggccgggctc ttcatcgacc cgcgcgaccc ggccctcggt gttcccctgc
       61 tctacgcgac gaccggtcgg accggcctcg acgtcttcga cctctcgcga ccacacgagc
      121 ccgtccggct cgggtcctgg agcggcgccg gcctcgccga cgtcgaggtc gtggcctcgc
      181 cggccgggcg gaccgtctac gcggcgaccg agtactggtt cggctcgacc acggccccac
      241 ggatcgtcga gctcgatgcc ggcgacctcg gcgcgatcgt ccagcgccgg cggttcagcc
      301 ccggcgaccc gccctacccc ggcgggatcc catggcgggt ccagggtctc gaggccgcgg
      361 gcgactacct ctacgtggcc cactcccacg cggggctcgg gatcctccac acctgctgct
      421 tcacggatcc gccacgcggc tcgacgaccg acctcggaga gggcaacacc gggggcgagc
      481 tcgggtccct ctccccgtac gcgatggacg tggagctcct gggcgagacg atcctcgtct
      541 ccgatgcctc gaccggcgtg ctcagcgcct tcgacttcga cccgatctgg cgccaccccg
      601 aggacgagct ctagtcccgc catcgcgcgg cgggccgcac gccagatttg tgcggttgca
      661 caaaggagcg cggccatgcc gcagagaacc ctcccggtag ccgacgaagg ggggacaccg
      721 gatgcgacgc gggctccgcg gcctgatctt gctttcgctc ctgctgggac tgctgccggc
      781 cacgagcatc gacgcccagg gcgtcgagtg cccgaccgtg gagccaccga agctggagtt
      841 cgacacgccc acctacatcg accgggatcg agccgggggc gagcccgtca gcatcgtcgc
      901 ggaggacggc tcgatcagcg tctccgccca cgccggcacg acccacatct acaagaaccc
      961 cgaggccgca ggcggtgccg gcgacttcct cgtcggctac acgaaccaga ccctcaactg
     1021 gcgctcggtc gacggcggca agacctggcg gtacatcggg ctcgccgggc tgcgggaagg
     1081 ccctcacacg gtgacctcga ccgggttctc cgaccccgac ttcgcgatgg acatgggcgg
     1141 gcgcatctac aacacggaga tcgacctcgc gaacgtggcg gtcttctcga gcgtcgacga
     1201 cggccagtcc taccccttcg gcaacccgga ggcggcgagc ggcgaccgcc cctggctgat
     1261 cggtggtgag cccgacgagg tctacctcta cgtcaacctg ccgaagacgt tctggcgttc
     1321 gaccaacggc gggctgacgt tctcgctgct gccgaccccg ccgatcaacg cgaagggcta
     1381 ccgcgacccg cgcaacgagc gcaacggtct gatcggcccc gtcggcacga acggcatcgc
     1441 gatctcctcg aacaagggcc agtcctggac gacgtacaac ggcgcgaacc tcgggccgaa
     1501 cgtcgacttc ttcgggtcgc tcgccgtcga caaggccggc cacgcctaca agatggccgc
     1561 gggcggatac tccggcagca ccgacacgac gcccaacggc atcgtcacct acaactactt
     1621 cgaccgcgac acgaaccgct ggggtcagcg cgtgacgatc ccgaccccgc cgggcgacgc
     1681 cctctggccg tggctcgtag ccggtgacga cggccgggtc gcgctgacct ggtaccagtc
     1741 cctcgcgggg aagccgcgcg agttctacgc cttcgtggcg tacacgcaca acgcccgcgg
     1801 gaccacggtg acgtgcgagg acgggagcac gaggttcatc ccgccgcagt tcaccgtcgt
     1861 gaacgcgagc cagcggccga tccacgtcgg cgcgatctgc ctcgacggga ccgcctgcaa
     1921 ctggaaccac ggcaccaact gggacaacgg cgaccgtcgg ctcggtgact tcttcacgat
     1981 caaccacgac cacgagggca acctgttcgt ggtcacgacc gacacgatgc tgaagagccc
     2041 gaccggtgga ccgaagcccg tcggcaaccc gctgttcatc aagcagaaga cgggcgacag
     2101 gatcctcgcg cagccggctc ccacgcggaa gacccgctgc ctgtggcccc tgccgagctg
     2161 ctgaggggta cgacgatgac gacgatccgg atgcggagcc tcagcatcgc cctcctgctc
     2221 gcggcggggc tcgccgtgcc ggcctcggcc gcggccccgc c
//
LOCUS       DATLGG010000299         5825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_155355, whole genome shotgun sequence.
ACCESSION   DATLGG010000299 DATLGG010000000
VERSION     DATLGG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155355"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VM638_08505"
     CDS             <1..530
                     /locus_tag="VM638_08505"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HVM12500.1"
                     /translation="LGRPWVRPHLVALVREVAEGMEFDLAALESRLRDLDSPDPTALA
                     EALGDPSELFGGEMTDERRLLLRRVQAFLAAAEGYADHVTDIAARDRLPSFGRIHEAV
                     RRRHEGRPEEERMVERLLGLDVVPEHYRLGRTFCDRVAEVTDEPTLARMWGSTDSMPS
                     MPELEEPTLWLARMA"
     gene            550..1329
                     /locus_tag="VM638_08510"
     CDS             550..1329
                     /locus_tag="VM638_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12501.1"
                     /translation="MSRAELVFANVLDGRHYDAEGVGGQPLVYVMSLPARATPFAVVR
                     HWKAPQGIATEHFELVAPSGRVAYASEPRARRFPGQMDLTELIDVVDDAVFGEIGIYV
                     ASFLIDGDVEGQVDFQVFLAAAPQKLPKDVEDALKKSDVIWVGAEGNGSHPVPTWFVY
                     RQGRIYVLHGSRDRGEQEIPGLPEAEEVTVVTRRKGRETRADRFPASVRLIPPQSPEF
                     DQVGAFLADRRRDRHIPPGQAIAAWRQAGLLIAELTPAIPA"
     gene            complement(1326..2267)
                     /locus_tag="VM638_08515"
     CDS             complement(1326..2267)
                     /locus_tag="VM638_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM12502.1"
                     /translation="MPLLDLGAAIAIGLRYFTIVYLAGLGGLFSERSGIVNIGLEGIM
                     IVGTVTGAWGTVTFGPVAGLAIGALSGFVYSMIHALATVTFRVDHIVSGVIINLIAIG
                     LARFLSQVFFGQATQSDPGIPRLDAWDIPLLGSLPLGLGRAFQAMSPVVILALLVTIP
                     AVWALNRTRFGLRLRAVGENPEAARTLGVRVAPLRYAGVGISGLLAGLAGAFLAVEIN
                     GLFREGQTQGLGFIALAALILGNWQPKRLLVAALLFGFAQAIPLRLSDAPIISLLPEQ
                     FIRMIPFVVTIIAIAGFVGRVHPPAAAGKAYEGAGGT"
     gene            complement(2267..3397)
                     /locus_tag="VM638_08520"
     CDS             complement(2267..3397)
                     /locus_tag="VM638_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVM12503.1"
                     /translation="MAKPAARRTAPPWLESLGGQALALLGAFVVAGLVGAVIIRAFGE
                     DPLFIYWTIWDFSTRRLSDVGRVLANATPLVFSGLAVAVAFKAGLFNIGVEGQYKVAM
                     MASSAAALHLDFLPGPVLVPIVIVTAMLAGTAWAAVPGILRVTTGAHEVVTTIMMNLI
                     AVSLVAWALLGPLRTSETGLIDLRTDIFAEHARIPHLAPTLGLDLPGSVQLSWLFPLA
                     ILACLVCWLLLYRMRLGYEVRAVGATAGAAEAGGISVGATQLKIFLVSGALAGLVGLN
                     HLLVDKGYLGSNYESGLGFAGIAVAFLGRNHPFGILLAAILVGALARGQDGVAVTSAL
                     PTEIIIILQGVLILAVVVAYEVVNRRLRSRRLRAAERAEAPA"
     gene            complement(3397..4920)
                     /locus_tag="VM638_08525"
     CDS             complement(3397..4920)
                     /locus_tag="VM638_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12504.1"
                     /translation="MGAREARNDEPLAVLEGITKRFPGVLADDRVDLTLRAGEIHALV
                     GENGAGKSTLMRVLYGLYPPDEGRILVRGREVRIASPRDAIALGIGMVHQHFMLVDRF
                     TVAENIVLGAEGGLVVDEAAAARRIDELAGAYGFEIDPVARVEDLSVGQEQRVEILKA
                     LYRGVDLLILDEPTAVLAPLEVEELFGNLRAMRDAGKTIVFISHKLDEVRSIADRITV
                     LRRGRVVGETTPAETSKERLAEMMVGRPVLFRLEKPRVERGEEILRLEGVEVDGALRG
                     LDLSVRAGEVVGVAGVEGNGQSALAEVVVGLRAPAAGRLLIRGRDAAGRPIDEIRSMG
                     VAYVPEDRHDRGLVLDMTLWENALLGKQSRGRYVRRFGFLSIRRIKELASRLVDRFDV
                     RARGIATQAATLSGGNQQKLILARELEDEPALLVAHQPTRGLDVGAIESVWRRILEEK
                     ARGAAVLLISSELDEIYALSDRIVTIYEGRLTGEYGPDAPAEEVGVGMTGGAASGAA"
     gene            complement(4931..>5825)
                     /locus_tag="VM638_08530"
     CDS             complement(4931..>5825)
                     /locus_tag="VM638_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HVM12505.1"
                     /translation="FNDAAKRGLDMAIDEGLVSDADCIEPNATGSNRDDNVVNLADQE
                     YELVAGIGFAFSEGIAKIAPDYPEQYFAVIDGFATTIATDATNIVDLAFKEHEGSFLV
                     GAAAAMASESGTIGFLGGQRGTGLIEKFEAGYEAGAREINPDIEILVEYIGDDVTAFN
                     DPTKGEALSQKMYDQGADVVYHAAGASGAGLFQAAVAADALAIGVDSDQYLTASPEQQ
                     PHILTSMLKRVDTAIHDVIADVKNGTFEPGFKVFGMAEEGVDYSQSNTELMTPEMIQR
                     LDEYKQQIIDGTITVPEEPQG"
BASE COUNT          874 a         2104 c         2048 g          799 t
ORIGIN      
        1 ccctgggacg gccctgggtc cgcccgcacc tcgtcgcgct ggtgcgtgag gtggccgagg
       61 ggatggaatt cgatctggcc gcgctcgagt cgcggctccg cgacctcgac tcccccgatc
      121 ccaccgcgct ggcagaggcg ctcggcgacc cgagcgagct cttcggcggc gagatgaccg
      181 acgagcgccg cctgctgctc cggcgggtgc aggcgttcct cgccgcggcc gaggggtacg
      241 cggatcacgt gacggacatc gcggctcgcg accggctccc gtccttcggg cggatccacg
      301 aggccgtgcg acgccggcac gaaggccgac cggaggagga gcggatggtc gagcgcctcc
      361 tcggcctcga cgtcgttccc gagcactacc ggctcgggcg gacgttctgc gaccgcgtcg
      421 ccgaggtgac cgacgagccg acgctcgctc ggatgtgggg ctcgacggac tcgatgccgt
      481 cgatgccgga gctcgaggag ccgacgctct ggctcgctcg catggcctga cctcggctat
      541 cctgccgacg tgtccagggc cgagctcgtg ttcgcgaacg tcctcgacgg ccgtcactac
      601 gatgccgaag gcgtcggagg ccagccgctc gtctacgtga tgagcctccc ggcgcgcgcg
      661 acgccgttcg cggtcgtgcg ccactggaag gcgccacagg ggatcgccac ggagcacttc
      721 gagctggtgg ccccctcggg ccgcgtcgcg tacgcgagcg agccgcgggc caggcgcttc
      781 cccgggcaga tggacctcac cgagctgatc gacgtcgtcg acgacgcggt cttcggggag
      841 atcgggatct acgtcgcgtc gttcctgatc gacggcgacg tcgaggggca ggtcgacttc
      901 caggtgttcc tcgcggcggc tccgcagaag ctgccgaagg acgtcgagga cgcgctgaag
      961 aagtccgacg tgatctgggt cggcgccgag ggcaacggca gccacccggt ccccacgtgg
     1021 ttcgtctacc gccagggccg gatctacgtg ctccacggct cgcgcgatcg cggcgagcag
     1081 gagatccctg gcctgccgga agccgaagag gtgaccgtgg tcacgcgccg gaagggacgg
     1141 gagacgcggg cggaccgctt ccccgcgtcg gtccggctga tccccccgca gagccccgag
     1201 ttcgaccagg tgggcgcctt cctggccgac cgccgccgcg accgccacat cccgccgggc
     1261 caggcgatcg cggcctggcg ccaggccggc ctcctgatcg ccgagctgac ccccgcgatc
     1321 ccggcctagg tgccgccggc gccttcgtag gccttcccgg cggcggccgg aggatggacg
     1381 cgtcccacga agccggcgat cgcgatgatc gtcacgacga acgggatcat cctgatgaac
     1441 tgctcgggca ggagcgagat gatcggcgcg tcggagaggc gcagggggat cgcctgggcg
     1501 aagccgaaca gcagcgccgc gacgaggagg cgcttcggct gccagttccc caggatcagc
     1561 gcggcgagcg cgatgaaccc gagtccctgc gtctgcccct cgcggaagag gccgttgatc
     1621 tcgacggcga ggaacgcccc ggccaacccg gcgagcagtc ccgagatccc gacgcccgcg
     1681 tagcggagcg gcgcgacgcg cacgccgagc gtccgcgccg cctcggggtt ctcgccgacg
     1741 gcgcggagcc ggagtccgaa ccgcgtgcgg ttgagggccc acacggccgg gatcgtcacg
     1801 agcagggcga ggatcacgac cggcgacatc gcctggaacg cgcgcccgag cccgagcggg
     1861 agcgatccga ggagcgggat gtcccacgcg tcgaggcgtg ggatgcccgg atcggactgc
     1921 gtcgcctgcc cgaagaagac ctgcgagagg aagcgcgcga ggccgatcgc gatcaggttg
     1981 atgatcacgc ccgagacgat gtggtcgacc cggaaggtca ccgtcgcgag ggcgtggatc
     2041 atcgagtaga cgaagccgga cagggcaccg atcgcgaggc cggcgacggg gccgaacgtg
     2101 accgtccccc acgccccggt gacggtcccg acgatcatga tcccctcgag cccgatgttc
     2161 acgatgccgg agcgctcgct gaacaggccg ccgaggccgg cgaggtagac gatcgtgaag
     2221 taccgcaggc caatcgcgat cgcggcgccg aggtcgagga gcggcatcag gccggcgcct
     2281 ccgcccgctc ggcggcccgc agccgccggc tccgcagccg ccggttcacg acctcgtacg
     2341 cgaccacgac cgcgaggatc agcacgccct ggaggatgat gatgatctcc gtgggcaagg
     2401 cgctcgtgac ggcgacgccg tcctgtccgc gtgcgagcgc cccgacgagg atcgccgcga
     2461 gcaggatccc gaacggatgg ttccggccga ggaaggcgac cgcgatcccg gcgaacccga
     2521 gcccgctctc gtagttcgac ccgaggtagc ccttgtcgac cagcaggtgg ttcagcccga
     2581 cgagcccggc gagcgccccg gagacgagga agatcttcag ctgggtggcg ccgaccgaga
     2641 tgccaccggc ctccgcggcc ccggccgtgg cgccgaccgc gcgaacctcg tagccgaggc
     2701 gcatccggta cagcaggagc cagcagacga ggcacgcgag gatcgcgagc gggaagagcc
     2761 acgagagctg gaccgaaccg gggaggtcga ggccgagcgt gggcgcgagg tgcgggatgc
     2821 gcgcgtgctc cgcgaagatg tcggtgcgca ggtcgatcag cccggtctcg gaggtccgca
     2881 gcgggccgag cagggcccac gccacgaggc tgaccgcgat caggttcatc atgatcgtcg
     2941 tcacgacctc gtgtgcgccc gtcgtcacgc gcaggatccc cggaacggcc gcccaggccg
     3001 tccccgcgag catcgccgtc acgatcacga tgggaacgag gaccgggccc ggcaggaagt
     3061 cgaggtggag ggccgcggcc gacgacgcca tcatcgcgac cttgtactga ccctcgacgc
     3121 cgatgttgaa gagccccgcc ttgaaggcca cggcgacggc gagtcccgag aagacgaggg
     3181 gcgtcgcgtt cgcgaggacg cgcccgacgt cggagaggcg gcgcgtcgag aaatcccaga
     3241 tcgtccagta gatgaacagc gggtcctcgc cgaacgcccg gatgatgacc gcaccgacga
     3301 gaccggcgac gacgaacgcg ccgaggagcg cgagcgcctg gccgccgagc gactcgagcc
     3361 acggtggcgc cgtgcgtcga gccgccggct tcgccatcag gcggccccgc tcgccgcgcc
     3421 gccggtcatc ccgaccccga cctcctcggc cggcgcgtcg gggccgtact cgcccgtcag
     3481 ccgcccctcg tagatcgtca cgatgcgatc cgacaacgcg tagatctcgt cgagctccga
     3541 agagatcagc aggaccgccg cgccgcgcgc cttctcctcg aggatgcgcc gccacaccga
     3601 ctcgatggcc ccgacgtcca ggccgcgcgt cggctggtgc gcgacgagca gcgccggctc
     3661 gtcctcgagc tcccgcgcga ggatcagctt ctgctggttg ccccccgaca gcgtcgccgc
     3721 ctgggtcgcg atccctcgag cccggacgtc gaagcgatcg acgaggcgag atgcgagctc
     3781 cttgatccgg cggatcgaca ggaagccgaa gcgccgcacg tagcggccgc gggactgctt
     3841 cccgagcagc gcgttctccc acagcgtcat gtcgaggacg aggccgcggt cgtggcggtc
     3901 ctcggggacg tacgcgacgc ccatcgagcg gatctcgtcg atcggtcgcc cggcggcgtc
     3961 gcgccctcgg atcaggaggc gtcccgcggc cggggcacgg aggccgacca ccacctccgc
     4021 gagcgccgat tgtccgttcc cctcgacgcc cgccaccccg acgacctcgc ccgctcggac
     4081 cgaaaggtcc aagccgcgca gcgcaccgtc cacctccacg ccctcgagcc gcaggatctc
     4141 ctccccccgc tcgacgcgcg gcttctcgag gcggaacagt accggccggc cgaccatcat
     4201 ctcggcgagc cgctccttcg acgtctccgc gggggtcgtc tcgcccacga cccgcccgcg
     4261 ccggaggacc gtgatgcggt ccgcgatgga gcgcacctcg tcgagcttgt ggctgatgaa
     4321 cacgatcgtc ttccccgcgt cccgcatcgc gcggaggttc ccgaacagct cttcgacctc
     4381 gagcggcgcg aggacggccg tcggctcgtc gaggatcagc aggtcgacgc cgcggtacag
     4441 cgccttgagg atctcgaccc gctgctcctg cccgaccgac aggtcctcca cccgcgcgac
     4501 cgggtcgatc tcgaagccgt acgcgcccgc gagctcgtcg atccgccggg cggccgcggc
     4561 ctcgtcgacg accaggcccc cttcggcgcc caggacgatg ttctcggcga cggtgaagcg
     4621 gtcgacgagc atgaagtgct ggtgcaccat cccgatgccg agggcgatcg cgtcgcgggg
     4681 ggatgcgatg cggacctcgc gcccgcgcac gaggatgcgc ccctcgtccg gcgggtacag
     4741 gccgtagagg acccgcatca gcgtcgactt gcccgcgccg ttctccccga cgagggcgtg
     4801 gatctcgccg gcgcgcaggg tcaggtcgac gcggtcgtcc gcgaggacgc ccgggaaacg
     4861 cttcgtgatc ccctcgagga ccgcgagcgg ctcatcgttc cgcgcttccc gggcgcccac
     4921 cgccgtgccg ctagccctgt ggctcctcgg gcacggtgat cgtcccgtcg atgatctgct
     4981 gcttgtactc gtcgagccgc tggatcatct ccggcgtcat cagctccgtg ttcgactgcg
     5041 agtagtcgac gccctcctcg gccatcccga acaccttgaa gccgggctcg aacgtcccgt
     5101 tcttcacgtc ggcgatgacg tcgtggatcg cggtgtcgac gcgcttcagc atcgacgtga
     5161 ggatgtgcgg ctgctgctcc gggctcgccg tgaggtactg gtcggagtcg acgccgatcg
     5221 cgagcgcgtc cgcggcgacc gccgcctgga agaggcccgc gcccgacgcg cctgccgcgt
     5281 ggtacacgac gtccgcgccc tggtcgtaca tcttctgcga cagcgcctcg cccttcgtcg
     5341 ggtcgttgaa cgccgtcacg tcgtcgccga tgtattcgac gaggatctcg atgtccgggt
     5401 tgatctccct cgcgcccgcc tcgtagccag cctcgaactt ctcgatcagg ccggtgccgc
     5461 gctgcccgcc gaggaacccg atcgtgccgg actcgctcgc catcgccgcc gcggccccca
     5521 cgaggaacga gccctcgtgc tccttgaagg cgaggtcgac gatgttcgtc gcgtcggtcg
     5581 cgatcgtcgt cgcgaagccg tcgatcaccg cgaagtactg ctcggggtaa tcgggcgcga
     5641 tcttcgcgat gccctccgag aacgcgaacc cgatgccggc gacgagctcg tactcctggt
     5701 cggccaggtt cacgacgttg tcgtcacggt tcgagcccgt ggcgttcggc tcgatgcagt
     5761 cggcgtcgga gacgagtccc tcgtcgatcg ccatgtcgag gccgcgcttc gccgcgtcgt
     5821 tgaac
//
LOCUS       DATLGG010000300         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_217282, whole genome shotgun sequence.
ACCESSION   DATLGG010000300 DATLGG010000000
VERSION     DATLGG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217282"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VM638_08535"
     CDS             <1..459
                     /locus_tag="VM638_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVM12506.1"
                     /translation="GAGALVVYLLDPQRGKARRHQLRDRSTAVARQAGRRVARAGRYA
                     GATAVGLSRRLRHSRSGQPYAAPNDQTLAAKVESEVFRRVQVPKGSININVEEGVVVL
                     RGEVGDAKQIAALEKAVNAVDGVRGVDNLLHLPGQPAPNKVEAQRVGFPT"
     gene            complement(490..1026)
                     /locus_tag="VM638_08540"
     CDS             complement(490..1026)
                     /locus_tag="VM638_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12507.1"
                     /translation="MPNEDMRTVVAVYPDLEHARKGVHALEAAGIDGANIHLEGGSAD
                     AAQRQGDTGDRDAGVVKGVASRAGRFGLIGAFAGGLVGFLAGLPFFGPLGLIASAVGA
                     AGAIGAFSAFVGGMSKLSMSEDWDVAHEGGGGEARLRLETASDEEAAAGMKVLRGTDP
                     VRVVDLTKEEEPARRPGS"
     gene            1098..>3187
                     /gene="pcrA"
                     /locus_tag="VM638_08545"
     CDS             1098..>3187
                     /gene="pcrA"
                     /locus_tag="VM638_08545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HVM12508.1"
                     /translation="MDSPLLAPLNPVQREAVVHTEGPSLIVAGAGSGKTRILTHRIAY
                     LLRERGVPPGSILAITFTNKAAQEMADRVEGLVGSRVAKAMWVLTFHSACARIMRREH
                     AHLGLPSTFTIYDEADSERVLRMVLKEMNVDEKRFPARAVKAAIGRAKDELLSPQQVA
                     ERAGNWYERMVAQAYQGYQQRLAGAGAVDFDDLILRTVQLFRGRPEVLEHYQERFRYI
                     HIDEYQDTNRAQYHMVNLLAAKYRNLCVVGDADQGIYSWRGATIRNLLDFESDYPDAR
                     EFILDQNYRSTRTILEVANALIEHNLQRKPKNLWTESEGGHPVVRHRADNEHDEAFFV
                     ATEVERLVQDEADCSYADVAVFYRTNAQSRVLEDVFMKAGIPYRVIGGLRFYERKEIK
                     DVVAYLKVLVNPRDVVSARRAINTPKRGIGDQTVAALESFARDEGITLVEAARRAEEI
                     AVLSPRALGAVRGFVQVMDGLEAKIRDGAGPATMIEAAFSDTGYWAELEAERTVEAET
                     RKENLKEFVGVGAEFQARQPDAGLPEFLEQIALVSEQDEYDESAGSVSLMTMHNAKGL
                     EFEVVFIVGMEDGVFPHYRSMTDAAELEEERRLAYVGITRARRRLYLTHAWSRSLFGA
                     SSYNPPSRFLGEIPAELIEEVGDETTVEARAAQAQVEVPDVAAGDTVRHEIFGEGVVV
                     SISGMGRDAEALVM"
BASE COUNT          488 a         1105 c         1132 g          462 t
ORIGIN      
        1 ggtgccgggg cgctcgtcgt gtacctgctc gatccccagc ggggcaaggc acgccgccac
       61 cagctccgcg accgctccac ggccgtcgcc cgccaggcgg gtcgccgcgt ggcccgggcg
      121 ggtcgttacg cgggagcgac cgcggtgggc ctgtcgcggc gtctccgcca ttcgaggagc
      181 ggccagccct atgcggcgcc gaacgaccag acgctcgccg cgaaggtgga gagcgaggtc
      241 ttccggcggg tccaggttcc gaagggctcg atcaacatca acgtcgagga gggcgttgtc
      301 gttctccgcg gcgaggtggg ggacgcgaag cagatcgcgg cgctcgagaa ggccgtcaac
      361 gcggtcgatg gcgtccgcgg cgtcgacaac ctgctccacc tgccgggtca gccggcgccg
      421 aacaaggtcg aggcgcagcg ggtcggcttc cccacctgag cttgggacgc gcgtctccgg
      481 gcggctgggt cagctccccg gccggcgcgc cggctcttcc tccttcgtga ggtcgaccac
      541 ccgcacgggg tcggtcccgc gcaggacctt catcccggcg gccgcctctt cgtccgaggc
      601 ggtctcgagc cgcaggcgtg cctcgccgcc gccgccctcg tgcgcgacgt cccagtcctc
      661 cgacatcgag agcttcgaca tgccccccac gaacgccgag aaggcgccga tcgcgccggc
      721 ggcgccgacc gcggacgcga tcaggccgag cggtccgaag aaggggagcc ccgcgaggaa
      781 gcccacgagc cctcccgcga acgcgccgat gaggccgaag cggccggcac ggctcgccac
      841 gcccttcacc acgccggcgt cacggtcgcc ggtgtctccc tggcgctgcg ccgcatcggc
      901 ggacccgcct tcgaggtgga tgttcgcccc atcgatgccc gctgcctcga gcgcgtggac
      961 ccccttgcgt gcgtgctcga ggtccgggta gaccgcgacc accgtcctca tgtcctcgtt
     1021 cggcatcctc atcgcctcct tcgctcggtg ccttcccggc cgaaggaacc gcgaaacgcg
     1081 gttgtatcat cggcgcgatg gactcccccc tcctcgcgcc gctcaatccc gttcagcgcg
     1141 aggcggtggt gcatacggag ggcccgtcgc tcatcgtcgc cggggccggc tccggcaaga
     1201 cgaggatcct gacccaccgc atcgcctacc tcctgcgcga gcgcggtgtc ccaccggggt
     1261 cgatcctcgc gatcaccttc acgaacaagg cggcccagga gatggccgac cgcgtcgagg
     1321 gcctggtggg ctcgcgggtc gcgaaggcga tgtgggtgct gaccttccat tccgcgtgcg
     1381 cgcgcatcat gcggcgggag cacgcgcatc tcggcctccc gtcgacgttc acgatctacg
     1441 acgaggccga ctccgagcgc gtcctccgga tggtgctgaa ggagatgaac gtcgacgaga
     1501 agcgcttccc ggcgcgagcc gtgaaggcgg cgatcggacg ggcgaaggac gagctcctgt
     1561 cgccccagca ggtcgcggag cgcgcgggca actggtacga gcgcatggtc gcgcaggcgt
     1621 accagggcta ccagcagcgc ctggcgggcg cgggtgccgt cgacttcgac gacctgatcc
     1681 tgcggaccgt ccagctcttc cggggtcgtc ccgaggtcct ggagcactac caggagcggt
     1741 tccgctacat ccacatcgac gagtaccagg acacgaaccg ggcgcagtac cacatggtga
     1801 atctgctggc ggcgaagtac cggaacctct gcgtcgtcgg cgacgccgac caggggatct
     1861 actcctggcg tggcgcgacg atccgcaacc tcctcgactt cgagagcgac tacccggacg
     1921 cgcgcgagtt catcctcgac cagaactacc gctcgacccg cacgatcctc gaggtggcga
     1981 acgcgctgat cgagcacaac ctccagcgga agccgaagaa cctgtggacg gagtcggagg
     2041 gcgggcaccc cgtcgttcga catcgggcgg acaacgagca cgacgaggcg ttcttcgtcg
     2101 cgaccgaggt cgagcgcctc gtccaggacg aggcggattg ttcctacgcc gacgtcgcgg
     2161 tcttctaccg gacgaacgcc cagtcccgcg tcctcgagga cgtcttcatg aaggccggca
     2221 tcccgtaccg cgtgatcggc ggcctccgct tctacgagcg gaaggagatc aaggacgtcg
     2281 tcgcgtacct caaggtgctg gtgaatccgc gggacgtcgt ctccgcgcgc cgggcgatca
     2341 acacgccgaa gcgggggatc ggagatcaga cggtcgcggc gctcgagtcg ttcgcgcgcg
     2401 acgagggaat cacgctcgtc gaggccgctc ggcgcgcgga ggagatcgcc gtgctgtctc
     2461 ctcgcgcgct cggggccgtc cgggggttcg tccaggtgat ggacgggctc gaggcgaaga
     2521 tccgtgacgg agccgggccg gcgacgatga tcgaagcggc cttctccgac accggctact
     2581 gggcggagct cgaggcggag cgcacggtcg aggccgagac ccggaaggag aacctcaagg
     2641 agttcgtcgg cgtcggggcg gagttccagg cgcgccaacc ggatgcggga ctgcccgagt
     2701 tcctcgagca gatcgcgctc gtgagcgagc aggacgagta cgacgagtcg gccgggtccg
     2761 tctcgctcat gacgatgcac aacgcgaagg ggctcgagtt cgaggtcgtg ttcatcgtcg
     2821 ggatggagga cggggtgttc ccgcactacc gctcgatgac cgatgccgcc gagctcgagg
     2881 aggagcggcg gctcgcgtac gtggggatca cgcgggcgcg ccgacggctc tacctgacgc
     2941 acgcctggag ccggtcgctc ttcggcgcct cgagctacaa ccccccgtcg cgcttcctcg
     3001 gcgagatccc ggcagagctg atcgaggagg tcggggacga gacgacggtc gaggctcggg
     3061 cagcgcaggc gcaggtcgag gttcccgatg tcgccgccgg cgacacggtg cgccacgaga
     3121 tcttcggcga gggcgtcgtc gtctcgatct ccgggatggg ccgcgacgcc gaggcgctgg
     3181 tcatgtt
//
LOCUS       DATLGG010000301         5496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_155574, whole genome shotgun sequence.
ACCESSION   DATLGG010000301 DATLGG010000000
VERSION     DATLGG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155574"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VM638_08550"
     CDS             complement(<1..214)
                     /locus_tag="VM638_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12509.1"
                     /translation="MARIVDLRRGDPLPTLQADVLDPEVVDGARSIIERVRAEGDAAL
                     VEMTRRYDGADIEGRIRVDDAEVARAT"
     gene            290..1792
                     /locus_tag="VM638_08555"
     CDS             290..1792
                     /locus_tag="VM638_08555"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVM12510.1"
                     /translation="MKTLYRATRVHAPPLGGEWVLVDERHVERVGAGDPPAADEVVDL
                     AGATIMPGFIDAHVHLSGTGMAESGLSLHGTRSREEVLDAVRRHLSERPGPLLAQGFD
                     ESGWNRPDLPTLQELDAISREPLILVRIDGYLSIVNSAALEGSGAAQMPEVERDASGS
                     ATGRLRDDANAHVQRWHFESLTDLDIRDAQLRAARLAAERGVTCVHEMAIPDKRGMRD
                     VEVLLAMRPDLPVHVVPYVAVQDLSFAIGRGLGQIGGDLFLDGSIGARTAALLAPYED
                     ADSAGSLAYEDDDLAEFLHNAHLAGLQVGLHVIGDAAIEQALRVWERVYQSLDSRNRR
                     HFRARRHRLEHFEMADQRRVERAAALGLAISIQPAFDAAWGQRGGMYEQRLGPERAAG
                     MNPFRDLLARGLEVGAGSDTPVTELDPMLGIWALETHHDPSQRLARAEAITLYTRGAA
                     RLGHLQKRGRLEPGAAADLAVFEVDPLGVPDVRGLRPIVTVAEGRVVFQR"
     gene            1773..2702
                     /locus_tag="VM638_08560"
     CDS             1773..2702
                     /locus_tag="VM638_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVM12511.1"
                     /translation="MSSSSAEAAPTRRLDPGFLLFLTPVLWGATFPAAKLGLEVIGVH
                     PFMAWTRLIGFVTILAGIPLVARGQVTRRALRAALLPGLLLGGFIFVAYVLQTEGLAR
                     TTATNAGFITGLYVVFTPVLALALFRQPAGLPAWVAVVVSVLGLALLSVPALDDLQVR
                     PGDLLVLLSAVAWAAHVVGVGRLARRHPAAVLSLAQMGAAAGFHLVATIGPGLHPAGA
                     VEVWHLLVVTGVLGSGLAYTLQVMAQREVTAGRAVVILAGESVAAAAFAAVWIGERLA
                     VHQWVGAAIVLLAMAISELGARRAAASRIEPAT"
     gene            complement(2699..4291)
                     /locus_tag="VM638_08565"
     CDS             complement(2699..4291)
                     /locus_tag="VM638_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVM12512.1"
                     /translation="MQRYDAVVVGGGHNGLVAAAYLARGGRSVLVLERRPVLGGAAVT
                     EEPWPGYRVSTLSYVVSLLAPRIIRDLELERHGYHVYPIDPSYFSPFPDGSGLLVWEE
                     PAKAAAEIARFSKRDAEAYLRYEEDLDRLARLVRPLLLRIPPSAKLKGFADLREALWM
                     ARYLRGHRDLLPRFLDLMTMSVADFLDDYFEHDAIKGALAPGGVIGAWGGPMTPGSAY
                     VLLHHRMGAAAGGHGAWAFVRGGMGALSEAIASSARAAGAEIRTDAEVARIDVHRGRT
                     RGVELADGTRIGARVVLSGIHPQTTFLGMVGAEHLPDDLVEEMHRYRTRGASAKVNLA
                     LDALPDFRAMPGTELGPQHPEFIICPSIEYLERAWDAAKYGRWSERPMLDCVIPTTKD
                     DSLAPEGKHVLTAFVQYAPYGLAEGMSWAEERDALGDRVIETIAEHAPNVPDAVVHRQ
                     VVTPLDLEERYGLVGGNIFHGEMSLDQLFFLRPVPQAGTYRTPIRRLYLCGSGAHPGG
                     GVMGAPGYNAARVVLRDLGGIR"
     gene            4322..5227
                     /locus_tag="VM638_08570"
     CDS             4322..5227
                     /locus_tag="VM638_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HVM12513.1"
                     /translation="MTRRLLLDTSSLMYRAYFALPDSIRDARGRPVNAVHGYLDMTAR
                     LIRNREPRGLVHVYDADWRPAFRVEAYEGYKANRLGQEEPDDLGPQFEILEEVLEAVG
                     MERGWSRGWEAEDAIGSLCAGARGRTRLEIVTGDRDLIQLVRDPKVRLLFTRRGVSEL
                     DVLDEAAVAERYGVPADRYAEFAILRGDPSDGLPGVRGVGEKTARALIAAYPSLDALV
                     EDATSERRSQAILQRSPALRARIADHVGYIRAMQTVVPIRTDLEVEVWRGQRDDAALE
                     RLARRLKLAGPIRRLRDVLDGTSRA"
     gene            5260..>5496
                     /locus_tag="VM638_08575"
     CDS             5260..>5496
                     /locus_tag="VM638_08575"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HVM12514.1"
                     /translation="MGVDGPFDEILSRASEGDPTAFADLYRAYAPGVLGYLRAQGASD
                     AEDLMGEVFVSVARRIASFEGDERKFRSWIFTVAH"
BASE COUNT          710 a         2015 c         1985 g          786 t
ORIGIN      
        1 ccgtcgcccg ggcgacctcg gcgtcgtcga cccggatccg accctcgatg tcggcgccgt
       61 cgtaccgtcg ggtcatctcc acgagcgcgg catccccctc cgcccggacg cgctcgatga
      121 tcgagcgggc gccgtccacg acctccgggt cgaggacgtc cgcctggagg gtgggcaggg
      181 ggtcgccccg ccggaggtcc acgatgcgcg ccataccggc atcctaaggg gtgacctggg
      241 agaacgggcc ggtaggatag ccggtgcccc cgagcggagg tccgaaacgg tgaagacgct
      301 ctaccgcgcc acgcgggtcc atgccccgcc actcggcggc gagtgggtcc tcgtcgacga
      361 acgccacgtg gagcgggtcg gcgcgggcga tccgcctgcg gcggacgagg tcgtcgacct
      421 ggcgggcgcg acgatcatgc cgggcttcat cgatgcgcac gtccacctct ccgggaccgg
      481 gatggcggag agcggtctga gcctgcacgg gacgcgctcg cgggaggagg tcctcgacgc
      541 ggtgcggcgg cacctctcgg agcggccggg accgctcctc gcgcaggggt tcgacgagag
      601 cggctggaac cgtccggatc tcccaaccct ccaggagctc gacgcgatct cgcgggagcc
      661 gctgatcctc gtccggatcg acggctacct ctccatcgtc aacagcgctg cgctcgaggg
      721 ctcgggcgcc gcgcagatgc cggaggtgga gcgcgacgcc tcggggtcgg cgaccggccg
      781 cctgcgggac gacgcgaacg ctcacgtcca gcgctggcac ttcgagtcgc tgacggacct
      841 cgacatccgc gacgcgcagc tgagggccgc acggctggca gccgagcgcg gcgtgacgtg
      901 cgtgcacgag atggcgatcc cggacaagcg cggcatgcgc gacgtcgagg tcctcctcgc
      961 gatgcgtccc gatctcccgg tccacgtcgt cccctacgtc gcggtgcagg atctctcgtt
     1021 cgcgatcggt cgcggcctcg ggcagatcgg cggggacctc ttcctcgacg gttcgatcgg
     1081 cgcgcggacg gcggcgctcc tcgctcccta cgaggacgcc gactcggcgg ggtccctcgc
     1141 ctacgaggac gacgacctgg ccgagttcct gcacaacgcc cacctcgcgg ggctccaggt
     1201 cggcctccat gtgatcgggg acgccgcgat cgagcaggcg ctccgcgtct gggagcgggt
     1261 ctaccagtcg ctcgactcgc gcaaccggcg ccatttccgc gcgcgtcgcc accggctcga
     1321 gcacttcgag atggccgatc agcggcgggt cgagcgcgcc gccgcgctcg gcctcgcgat
     1381 ctcgatccag ccggcgttcg acgccgcgtg ggggcagcgc ggtgggatgt acgaacagcg
     1441 cctcggaccg gagcgcgcgg cggggatgaa cccgttccgc gacctgctcg cccgcggcct
     1501 cgaggtgggg gcgggatcgg acacccccgt gacggagctc gatccgatgc tcggcatctg
     1561 ggccctcgag acgcatcacg acccgtcgca gcggctcgcc agagccgagg cgatcaccct
     1621 ctacacgcgg ggcgcggccc gactcggaca cctgcagaag cgcggcaggc tcgagccggg
     1681 agccgccgcg gacctcgccg tcttcgaggt cgacccgctg ggggtccccg acgtccgtgg
     1741 tctgcgcccg atcgtgaccg tcgcggaggg gcgtgtcgtc ttccagcgct gaggcggccc
     1801 ccacgcgccg cctcgacccg gggttcctcc tgttcctgac gccggtgctc tggggggcga
     1861 cgttccccgc agcgaagctc gggctcgagg tgatcggcgt gcaccccttc atggcctgga
     1921 cgcggctgat cgggttcgtg acgatcctcg cgggcatccc gctcgtcgca cgtggccagg
     1981 tgacgcgtcg cgccctccgg gccgcgctgc tccccgggct gctcctcgga gggttcatct
     2041 tcgtcgccta cgtcctgcag accgagggcc tcgcccggac gaccgcgacg aacgccgggt
     2101 tcatcaccgg gctctacgtc gtcttcacgc ccgttctcgc gctggcgctc ttccggcagc
     2161 ccgccggtct tcccgcttgg gtggcggtgg tcgtctcggt gctcggcctg gccctcctgt
     2221 cggtccccgc gctcgacgac ctgcaggtgc gtccggggga cctcctcgtg ctgctctcgg
     2281 cggtcgcctg ggccgcccac gtcgtcggcg tcgggcggct ggcgcggcgg caccccgccg
     2341 ccgtcctctc cctcgcgcag atgggggccg ccgccgggtt ccacctcgtg gcgacgatcg
     2401 gccccggcct ccatcccgcg ggcgccgtcg aggtctggca cctgctcgtc gtgaccggcg
     2461 tgctcggctc gggcctcgcc tacacgctgc aggtcatggc gcagcgggag gtgaccgccg
     2521 ggcgagcggt cgtgatcctg gcgggggagt cggtcgcggc cgccgcgttc gccgccgtct
     2581 ggatcggcga gcgcctcgcg gtccaccagt gggtcggggc cgcgatcgtg ctcctcgcga
     2641 tggcgatctc ggagctcgga gcgcggcggg ccgccgcttc gcggatcgag cccgcgacct
     2701 agcggatccc gccgaggtca cgcaggacca cgcgcgccgc gttgtatccc ggcgcgccca
     2761 tcaccccgcc gccgggatgg gcgccggatc cgcagaggta cagccggcgg atcggcgttc
     2821 gatacgtccc cgcctggggg accggcctga ggaagaagag ctggtcgagg gacatctcgc
     2881 cgtggaagat gttcccgccg acgagcccgt accgctcctc caggtcgagg ggcgtcacga
     2941 cctggcggtg cacgaccgcg tccggcacgt tcggggcatg ctcggcgatc gtctcgatca
     3001 cccggtcgcc caaggcgtcg cgctcctcgg cccagctcat cccctcggcg agtccgtacg
     3061 gtgcgtactg gacgaaggcc gtcagcacgt gcttcccctc gggcgcgaga gagtcgtcct
     3121 tcgtcgtggg gatcacgcag tcgagcatcg gtcgctccga ccaccgtccg tacttcgcgg
     3181 catcccacgc acgctcgagg tactcgatcg acggacagat gatgaactcg ggatgctgcg
     3241 gcccgagctc cgtcccgggc atcgcgcgga agtcggggag cgcgtcgagc gcgaggttca
     3301 ccttggccga cgcgccgcgc gtccggtagc ggtgcatctc ctccacgagg tcatcgggca
     3361 ggtgctccgc tccgaccatc ccgaggaacg tcgtctgcgg gtggatcccc gacaggacga
     3421 cgcgagcacc gatccgcgtc ccgtcggcca gctcgacccc gcgggtccgc ccacggtgga
     3481 cgtcgatcct cgcgacctcg gcgtcggtcc ggatctcggc gcccgcggcg cgcgccgaag
     3541 aggcgatcgc ttccgagagc gcccccatgc ccccgcggac gaaggcccag gcgccgtgtc
     3601 cgccggcggc agcgcccatc cggtggtgca ggagcacgta ggcggagccc ggcgtcatgg
     3661 ggcctcccca cgccccgatc acgccaccgg gggcgagggc ccccttgatc gcgtcgtgct
     3721 cgaagtagtc gtcgaggaag tccgccaccg acatcgtcat gaggtcgagg aagcggggga
     3781 ggaggtcacg gtgcccgcgg aggtaccgcg ccatccacag cgcctcacgg aggtcggcga
     3841 accccttcag cttcgcgctc ggtgggatgc gcagcaggag cgggcggacg aggcgcgcca
     3901 accggtcgag atcctcctcg taccgcaggt acgcctccgc gtctcgcttc gagaaccgcg
     3961 cgatctcggc ggcggccttc gcgggttcct cccagaccag caggccgctc ccgtccggga
     4021 acggcgagaa gtaggagggg tcgatcgggt acacgtggta accgtgccgc tcgagctcga
     4081 ggtcgcggat gatgcgcggc gcgaggaggc tgaccacgta cgagagcgtc gagacgcggt
     4141 acccgggcca cggctcttcc gtgacggcgg cacccccgag gaccgggcgc cgctcgagga
     4201 cgagcaccga gcggccgccg cgcgcgaggt aggcggccgc cacgagcccg ttgtggcccc
     4261 cgccgacgac caccgcgtcg tagcgctgca ccgggtcatc ctacggccct aggctgtgcc
     4321 cgtgacgcgc cggctcctcc tcgacacctc gagcctgatg tatcgggcgt acttcgcgct
     4381 cccggattcg atccgagatg cgcgcggccg gccggtgaac gccgtccacg gctacctcga
     4441 catgacggcg cgcctgatcc gcaaccggga gccgcgaggc ctcgtgcacg tctacgacgc
     4501 cgactggcgc ccagcgttcc gcgtcgaggc gtacgagggc tacaaggcga accggctcgg
     4561 gcaggaagag ccggacgacc tcggtccgca gttcgagatc ctcgaagagg tcctcgaggc
     4621 ggtggggatg gagcgcggct ggtcccgggg gtgggaggcc gaggacgcga tcgggtcgct
     4681 ctgcgccggc gcccgtgggc ggacgcgcct cgagatcgtc accggcgacc gcgacctgat
     4741 ccagctcgtg cgcgacccga aggtgcgcct gctcttcacc cggcgcggcg tctccgagct
     4801 cgacgtcctc gacgaggcgg cggtcgccga gcgctacggc gtccctgcgg accgctacgc
     4861 cgagttcgcg atcctgcggg gggacccatc cgatgggctc cccggggtgc gcggcgtggg
     4921 ggagaagacg gcgagggcgc tcatcgcggc gtacccgagc ctcgacgcgc tcgtggagga
     4981 tgcgacgagc gaacgccgct cgcaggcgat cctgcagcgg tcacccgcgc tccgcgcgcg
     5041 gatcgccgat cacgtcggct acatccgcgc gatgcagacg gtcgtgccga tccggaccga
     5101 cctcgaggtc gaggtgtggc ggggccagcg ggacgacgcc gccctcgaac gcctcgcccg
     5161 ccgcctcaag ctcgccggcc cgatccgtcg cctccgcgac gtgctggacg gcaccagccg
     5221 cgcctgatcc ctcggcagaa cctcggcgat acttgggggg tgggcgtcga cgggccgttc
     5281 gatgagatcc tcagccgcgc gagcgagggc gaccccaccg ccttcgccga cctctaccgc
     5341 gcgtacgccc ccggtgtcct cggctacctc cgagcgcagg gcgcttcgga tgccgaggat
     5401 ctgatggggg aggtcttcgt ctcggtcgct cgccggatcg cctccttcga gggtgacgag
     5461 cgcaagttcc ggtcgtggat cttcacggtc gcccac
//
LOCUS       DATLGG010000302         5624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_106407, whole genome shotgun sequence.
ACCESSION   DATLGG010000302 DATLGG010000000
VERSION     DATLGG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106407"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1637
                     /locus_tag="VM638_08580"
     CDS             222..1637
                     /locus_tag="VM638_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HVM12515.1"
                     /translation="MARIGRTLVVSLVVGLVAGVPAAFASHKGDSLNDLVVTQSASPD
                     AVVEGEHVTYRIEVANRGPHPALEIQVNQVVSGGTIVSASGHRWECPSIEAGRASCRL
                     HGSLSPGGTSAPLLVVVQAAVDGGSQLSSTATALSHGTEINPPDNVSTLSVGVAPGTE
                     LGVSMYDTPDPVTSGANVKYGISVTNNGDASATGVSVTHQVSTGETTSFFGDGWDCAL
                     AARTASCALADPLGAGVSAPELSVVVRAPETSTRSSIRATASVSAANQTSAAEPAEQT
                     TTVDPAGQDFAIGYIPPEGGCLTTNPTGICTSVGGSGGDAFAPASSASSTPVDSGATP
                     TNNTFGDVSFEVGPGGVASLFEGSSQLAICAQLRPFEALNCAGAALDVIVPPGYDDRR
                     SPIAVNLVYDNSVAPASEDPRTVYAEKPVGGQTTTFILTPCGFSGFDGPTPCVDFQHR
                     IAGDDLLIRILMISQDPKYQG"
     gene            complement(1638..5408)
                     /locus_tag="VM638_08585"
     CDS             complement(1638..5408)
                     /locus_tag="VM638_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVM12516.1"
                     /translation="MDRAALLPYAPPLAHGWPGPVPGRREIAGSMAFVDVSGFTKMSE
                     RLARRGKVGAEEVAGVIDRCFSALLGVAHAEGGQLLKFGGDALLLFFSGEEHERRAAR
                     ATASMRRTLRSMARIPTSAGAVTLRMSVGVHAGSFTFSMVGRSHRELVVSGPAATRTV
                     EMESAAAAGQILLSPELASALPRVHRGRALGPGILLAGLPTVTRTPPSAAAGDGTAAE
                     RLVPAAIRDHVLAGGGESEHRLVSIAFLHFGGIDGLPADEALARLHEVIGEVQRIAQD
                     HDVTFLGTDIDRDGGKIILVAGAPATFPDDEGRLLRTVRAVLDAGLPLPLRAGVNRGH
                     VFSGDIGPPERRTYTVMGDAVNLAARLMQHAHDGEIVVAPEVVERARTGFEVEPLEPL
                     VVKGKARPVEALRLGAAHTRERRQDRGATPLIGREAELATLLEAVDDARAGRTRVVEI
                     VADAGLGKSRLVEEVARRSGLQTLRGGCDPYEAATPYFPFRTGLRTLLGIDEGAPERE
                     AIARLRSAVTEHAPSLLPWLSLIAAPLGLSVADSREVADLDEAFRRTKLHTVVAEVIA
                     EIVGGPLILLIEDAHWIDDASADLLRSLAAETERPWLICVTRRGGDDPLGLDAEATTR
                     LELVPLGGDASAALLAAATEDAPLHPHETRALAERAGGNPLFLAELLRARAAGEGGDA
                     EDLPDTVESAIAARIDRLPPRERDTLRRLSVLGPTFGIDLVEAVVETPETASVERLKG
                     FISPLDGRTLRFEHALIRDVAYERLPYKLRRELHARVAERIERAAFPDLDNHAEALAL
                     HFSEAGNAASAWIYARVAGRRAQTMYANVEAARFYERALRAARSLDVPDEVVADLWES
                     LGDVRARVGEFTAATAAYGRARRLHGSDVLAQANLLRKEAEVARRAGRFTDALRRLAR
                     GRGALAAVEGPEAARERARLSAWYASVRQEQGRSSDAVEWCHRAIEEAEPAGADDVLA
                     HASYLLDWAHVLEGRLDLATNSERALEIFERLGDLGGQANVLNNLGGFAYERGRWDEA
                     LAYYERARAVREKTGDAVNAAFGTINIGEILIDQGRFEEAERRVRDGLRVWRAAGYRS
                     GVGFALMLLGRVAAGLGRHVEALEHLDEARREFAYVGAAHDVVGVDARVAEVRLQMGD
                     AAGAIELAEDARTRLASIRPAAVHRPVILRVLGLARLADGDADGASQALEEALAAARA
                     AEREHEAALTLLALATLAESAGDAARAASLREEAAPTLERAGVRPPAGSTIP"
BASE COUNT          751 a         2094 c         2021 g          758 t
ORIGIN      
        1 ggacagggcg atgcggtgca tggctctcct ccctccctgt aggactggcg gagattgtac
       61 ggagattccg gacacgacgc aatggtccgt tccggggcgc gcttccaaca gggacctgct
      121 tcatcaccct tgtcgggcct gcgccgttcg cgtaccattc ctgctgttcg cagcgttcac
      181 ggccgcggcc ttccgccgcc gcctcaggga gggagagaac gatggcacgg atcggtcgca
      241 cgctcgtcgt ttcgttggtc gtggggctcg tggcgggggt tcccgccgcc ttcgcctcgc
      301 acaaggggga cagcctcaac gacctcgtcg tcacgcagtc cgcctcgcca gacgcggtcg
      361 tggaaggaga gcacgtcacc taccgcatcg aggtggcgaa ccgcgggccg cacccggccc
      421 tcgagatcca ggtgaaccag gtcgtctccg gtggcacgat cgtctccgcc tcgggtcatc
      481 gctgggagtg cccctcgatc gaggcgggcc gcgcttcgtg tcgtctccac gggtcgctct
      541 cgccgggtgg gacgtccgcc cccctgctcg tcgtggtgca ggcggcggtc gacgggggct
      601 cgcagctatc gagcaccgcg accgcgctgt cgcacgggac cgagatcaac ccgcccgaca
      661 acgtctcgac gctcagcgtt ggtgttgccc ccggcaccga gctcggcgtg agcatgtacg
      721 acacgccgga cccggtgacc tcgggtgcga acgtcaagta cgggatctcg gtcacgaaca
      781 acggcgacgc ctcggcgacg ggggtgagcg tcacgcacca ggtctccaca ggagagacca
      841 cctcgttctt cggtgacggt tgggactgcg cgctcgcggc gcggacggct tcgtgcgcgc
      901 tcgcggaccc gctgggagcg ggtgtctccg cgcccgagct gtcggtcgtc gttcgggcac
      961 ccgagacgtc gacccgcagc agcatccggg cgaccgcctc ggtgagcgcg gcgaaccaga
     1021 cctccgcggc cgagccggcc gagcagacga cgacggtcga cccggccggg caggacttcg
     1081 cgatcgggta catccccccc gagggcgggt gcctgacgac caacccgaca ggcatctgca
     1141 cgagcgtcgg gggatccggc ggtgacgcgt tcgcccccgc ctcctccgcc tcgtccactc
     1201 cggtggacag cggtgccacc ccgacgaaca acaccttcgg agatgtctcg ttcgaggtcg
     1261 ggcccggcgg cgtcgcgtcg ctcttcgagg gcagctcgca gctcgcgatc tgcgcgcagc
     1321 tccggccgtt cgaggcgctg aactgcgcgg gtgccgcgct cgacgtcatc gtcccgcccg
     1381 ggtacgacga ccgcaggagc ccgatcgccg tgaacctcgt ctacgacaac tcggtggcgc
     1441 ccgcgtcgga ggatccgcgc acggtctacg ccgagaagcc ggtcggcggc cagaccacga
     1501 ccttcatcct cacgccgtgt gggttctcgg ggttcgacgg gccgacccca tgcgtcgact
     1561 tccagcaccg catcgcgggg gacgacctct tgatccggat cctgatgatc tcccaggatc
     1621 cgaagtacca gggatagcta cgggatcgta gacccggccg gggggcggac gccggcgcgt
     1681 tcgagcgtcg gcgccgcctc ctcgcgcagg ctggccgcgc gtgcggcgtc gcccgcggac
     1741 tccgcgaggg tcgccagcgc cagcagcgtc agcgcggcct cgtgctctcg ctccgccgcg
     1801 cgcgccgccg cgagcgcctc ttcgagcgcc tgcgaggcgc cgtcggcatc cccgtccgcg
     1861 agccgcgcga gtccgagcac gcgcaggatc accgggcggt gcaccgcggc gggacggatc
     1921 gaggccagcc gcgtccgggc gtcttcggcg agctcgatcg cccccgcggc atcgcccatc
     1981 tgcaggcgga cctccgccac gcgggcgtcg acacccacca catcgtgcgc cgctcccacg
     2041 taggcgaact cgcgccgagc ctcgtcgagg tgctcgagcg cttcgacgtg tcgaccgagg
     2101 cccgccgcca cccgcccgag aagcatcagc gcgaagccca ccccgctccg atagcccgcg
     2161 gcgcgccaca cccgaagccc gtcgcggacc cggcgctccg cctcctcgaa ccgaccctgg
     2221 tcgatgagga tctcgccgat gttgatcgtc ccgaaggccg cgttgaccgc gtcgccggtc
     2281 ttctctcgga cggcgcgcgc ccgctcgtag tacgcgaggg cctcgtccca gcggcctcgc
     2341 tcgtaggcga acccgcccag gttgttcagc acgttcgcct gaccgcccag atcgccgagg
     2401 cgctcgaaga tctcgagcgc gcgctccgag ttggtggcca ggtccagccg cccctcgagg
     2461 acgtgcgccc agtccagcag gtacgacgcg tgcgcgagca cgtcgtcggc gccggcgggc
     2521 tcagcctcct cgatcgcgcg gtggcaccac tcgacggcgt cgctgctccg cccctgctcc
     2581 tggcgaaccg atgcgtacca ggcggacagg cgcgcgcgct cccgggcggc ctcggggcct
     2641 tcgacggccg ccagtgcgcc tcggccacga gcgaggcgcc ggagcgcgtc cgtgaagcgt
     2701 ccggcgcgcc gggcgacctc cgcctccttg cgcaggaggt tggcctgcgc gaggacgtcg
     2761 ctcccgtgga ggcggcgggc gcgcccgtag gcggcggtcg cggcggtgaa ctcgccgacc
     2821 cgcgctcgca cgtcgccgag cgactcccac aggtccgcca cgacctcatc cggcacgtcc
     2881 aggctccgtg cggctcgcag cgcccgctcg tagaaccgtg ccgcctcgac gttcgcgtac
     2941 atcgtctggg cgcgccggcc ggcgacgcga gcgtagatcc acgccgaggc cgcgttcccg
     3001 gcctccgaga agtggagcgc gagcgcttcg gcgtggttgt cgaggtcggg gaaggccgct
     3061 cgctcgatgc gctccgccac gcgcgcgtgc agctcacggc gcagcttgta ggggagccgc
     3121 tcataggcga cgtcgcggat cagggcgtgc tcgaaccgga gggtccgccc gtcgagcggg
     3181 gagatgaatc ccttgaggcg ctcgaccgag gcggtctcgg gggtctcgac cacggcctcg
     3241 accagatcga tcccgaaggt gggtcccagc accgacagtc gacgaagggt gtctcgctcg
     3301 cgcggaggga gccggtcgat gcgcgccgcg atcgcgctct cgaccgtgtc ggggaggtcc
     3361 tcggcgtcgc ctccctctcc ggccgcgcgt gcccgcagga gctccgcgag gaacagcggg
     3421 ttcccgcccg cacgctcggc cagcgcgcgc gtctcgtgcg ggtggagggg ggcgtcctcc
     3481 gtcgccgccg cgaggagggc cgcgctcgcg tcacccccga gcggaacgag ctcgagccgg
     3541 gtcgtcgcct cggcgtcgag cccgaggggg tcgtcaccgc cgcggcgggt cacgcagatc
     3601 agccacggcc gctccgtctc ggccgccagg gagcggagca ggtccgcgga ggcgtcgtcg
     3661 atccagtggg catcctcgat caggaggatc agaggccccc cgacgatctc cgcgatcacc
     3721 tcggcgacga cggtgtgcag cttcgtccgc cggaacgcct cgtcgagatc ggcgacctcc
     3781 cgtgagtcgg cgacggacag gcccaggggc gcggcgatga gcgagagcca tggaaggagc
     3841 gatggcgcgt gctccgtcac cgccgaacgc aggcgagcga tcgcctcccg ttcgggcgcg
     3901 ccctcgtcga tgccgagcag ggtgcggagg cccgtgcgga acgggaagta cggggtggcg
     3961 gcctcgtagg ggtcgcagcc tccgcgcagg gtctgcagcc cgctgcgtcg cgcgacctcc
     4021 tcgacgaggc gtgacttgcc gaggccggcg tcggcgacga tctcgaccac gcgcgtacgg
     4081 cccgcgcggg catcgtcgac cgcctcgagc aaggtggcca gctctgcctc gcggccgatc
     4141 agcggcgtcg ctccccggtc ctggcgccgc tcccgcgtgt gtgccgcccc gagccgcagc
     4201 gcctcgaccg gacgggcctt gcccttgacc acgagcggtt cgagcggctc gacctcgaag
     4261 ccggtccgcg cccgctccac gacctcgggg gcgacgacga tctcgccatc gtgcgcgtgc
     4321 tgcatcagcc gcgcggcgag gttcacggcg tcccccatga ccgtgtaggt ccgccgctcc
     4381 ggcgggccga tgtcgccgga gaagacgtgc ccgcggttca cccctgcgcg cagtggcagc
     4441 gggaggccgg cgtcgaggac cgctcgaacc gtccgcagga gccgcccctc gtcgtccggg
     4501 aaggtcgcgg gcgccccggc gacgaggatg atcttgccgc cgtcacggtc gatgtcggtc
     4561 ccgaggaacg tgacgtcgtg gtcctgcgcg atccgctgga cctccccgat gacctcgtgg
     4621 agccgcgcca gcgcctcgtc ggcgggcaga ccgtcgatcc ccccgaagtg gaggaaggcg
     4681 atggacacga ggcgatgctc gctctcgccg ccccccgcca ggacgtggtc gcggatcgcc
     4741 gccggcacga ggcgctcggc tgcggtgccg tcgccggcgg ccgccgacgg tggtgtccgc
     4801 gtcacggtcg gaagccccgc cagcaggatc ccggggccga gcgcccggcc gcggtggacg
     4861 cgggggagcg ccgaggcgag ctcggggctc agcaggatct gaccggctgc cgcggccgac
     4921 tccatctcga ccgttcgggt tgccgcggga ccggagacga cgagctcccg gtggctccgg
     4981 ccgaccatcg agaaggtgaa cgacccggcg tgcacgccca ccgacatccg cagggtcacc
     5041 gctccggccg aggtcgggat gcgcgccatc gagcggagcg ttcggcgcat cgaggccgtc
     5101 gcacgcgcgg cgcgtcgctc gtgctcctcg ccggagaaga agaggagcag ggcgtcaccg
     5161 ccgaacttca gcagctggcc gccttcggcg tgcgcgacgc cgaggagggc cgagaagcag
     5221 cggtcgatga cgccggcgac ctcctcggcg cccaccttgc ctcgccgcgc cagtcgctcg
     5281 gacatcttcg tgaagccgga gacgtcgacg aacgccatcg agccggcgat ctcccggcgt
     5341 ccggggacgg gccccggcca gccgtgcgcg agcggcggcg cgtacgggag cagggcagct
     5401 cgatccatgc gtccatcatg ggtggaacgc ccggatccaa caagggccga acggccctcg
     5461 gacccgggcg tcccccctag ccgagcgcga tgttgctgat cgagcaggcc tcgccgaggc
     5521 aggccttcgc ccagatctcc cgcgggccgc tgccgcgcgg gaggcgctgc ccgaaccgtc
     5581 cgcccgagac gtccgccggg acgaaggcga cctcgccggc cgac
//
LOCUS       DATLGG010000303         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_14456, whole genome shotgun sequence.
ACCESSION   DATLGG010000303 DATLGG010000000
VERSION     DATLGG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14456"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VM638_08590"
     CDS             complement(<1..243)
                     /locus_tag="VM638_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HVM12517.1"
                     /translation="MNRPRKLIASSLAVVVVATLWFLFAPRQIPGGSVSYVVPSGTSM
                     LPTYERGDVVILREASSYRPGDVVAYESDDLRRLVLH"
     gene            complement(319..687)
                     /locus_tag="VM638_08595"
     CDS             complement(319..687)
                     /locus_tag="VM638_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12518.1"
                     /translation="MGLRWRKRTLGGIVLAGLIGSSAYAMTAANTFSGDNASGSGSDT
                     ISGYEVSDVNYTLASDPEELEAVAFTLDGEASDVRAKVRAAASYATCEEVEGETNRWS
                     CSLSGESVSAAQSLSVIAVK"
     gene            complement(840..1487)
                     /locus_tag="VM638_08600"
     CDS             complement(840..1487)
                     /locus_tag="VM638_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM12519.1"
                     /translation="MTISVYILDDHALIRRGLRDLLDPEDDIEVIGESATVAEALAYI
                     DRTPPDVAVVDLRLPDGDGITACREIRSRHPATRCLILTSYGDEEAIVNAVVAGADGF
                     LIKDSEARVVLDAIRTVAESGSLLSPEMTRQVLERLRIGSGPRAEEGRLTKQQDRLLD
                     LLAEGLTNREIAERLHLSEKTVRNYVSNLLTQLGMSHRTQAALYAAERRRGRGDA"
     gene            complement(1614..1688)
                     /locus_tag="VM638_08605"
     tRNA            complement(1614..1688)
                     /locus_tag="VM638_08605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1651..1653),aa:Pro,seq:ggg)
     gene            1842..2054
                     /locus_tag="VM638_08610"
     CDS             1842..2054
                     /locus_tag="VM638_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVM12520.1"
                     /translation="MYVCLCMGIDDRAAREAIRAGASTVRDVLRACGSARPCGGCSPV
                     IRVLLHQASAADETSVIRIDQYREAR"
     gene            2051..>2433
                     /gene="bfr"
                     /locus_tag="VM638_08615"
     CDS             2051..>2433
                     /gene="bfr"
                     /locus_tag="VM638_08615"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HVM12521.1"
                     /translation="MKGNERVIETLNELLTLELTVINQYFLHSKMCEDWGYARLAAKL
                     RAVSREEMDDAEEIIDRVLFLDGVPNMQRLGPVTIGQTVPEQLRIHLDAERRALELLS
                     QGIGVADDAGDAASREFFASRLQEE"
BASE COUNT          389 a          846 c          826 g          372 t
ORIGIN      
        1 gtgaaggacc aggcgacgca gatcgtcgct ctcgtacgcc acgacgtcgc ctggccggta
       61 cgagctcgcc tcgcgcagga tgacgacgtc tcctcgttcg taggtcggca acatgctcgt
      121 gccgctcgga acgacgtacg agacggatcc gcccgggatc tgcctgggcg cgaacaggaa
      181 ccacagcgtg gccacgacca cgacagcgag tgagcttgcg atgagcttcc tgggccggtt
      241 catgacggat ccgtctcccc tccggatcgg agggggagcg gtcccgcgtg ctgcgaggcc
      301 gctccccctg cgtacgtgct acttgacggc gatcacggag agcgactggg cggccgagac
      361 gctctcgccg ctgagcgagc agctccagcg gttcgtctcc ccctcgacct cctcgcaggt
      421 cgcgtaggaa gccgccgccc ggaccttcgc ccggacatcc gacgcctccc cgtccagggt
      481 gaaggccacg gcctcgagct cctccgggtc gctggcgagc gtgtagttca cgtcgctcac
      541 ctcgtatccg gagatcgtgt cggagcccga ccccgaggcg ttgtcacctg agaaggtgtt
      601 ggccgcggtc atcgcgtaag ccgacgatcc gatcagccct gccaggacga tccccccgag
      661 cgtcctcttc ctccaacgga gccccatgct gtcccccttc gggtctgtcg ggtgctcgct
      721 gcacccgtct gctgccgatg ttcgacgaac gcgaggggcg tgcgtagagg aatgtgtcac
      781 cggttctgcg gtgacggccg tcccgagtgc cgacgaatca cccgaaagga cgcgggccgt
      841 caggcgtcgc cgcggccgcg ccggcgttcg gccgcataga gggccgcctg cgtgcgatgg
      901 ctcataccga gctgggtgag gagattcgac acgtagttcc ggaccgtctt ctcggagagg
      961 tgcaaccgct cggcgatctc tcggttcgtg agcccctcgg cgaggaggtc gaggagacgg
     1021 tcctgctgct tcgtcaggcg gccctcctcg gcgcggggcc cagacccgat ccggagccgc
     1081 tcgagcacct ggcgggtcat ctccgggctc agcagcgacc ccgactccgc cacggtgcgg
     1141 atggcgtcca ggaccaccct ggcctccgag tccttgatca ggaacccatc ggcacccgcc
     1201 acgacggcgt tcacgatggc ctcttcatcg ccgtacgacg tcaggatcag gcaccgtgtc
     1261 gctgggtggc gcgaacggat ctcgcggcag gcggtgatcc catcgccgtc cggaagtcgc
     1321 agatccacga ccgcgacgtc cgggggggtc cggtcgatgt acgcgagcgc ctcggcgacg
     1381 gtggccgatt caccgatcac ctcgatgtcg tcctcgggat cgaggaggtc gcgcagtccc
     1441 ctcctgatca gggcatgatc gtcgaggatg tagaccgaga tggtcacgga acaatcctac
     1501 tggccgcggc ggcgcccgga tagtcccgta cgcccgctac atccacgcga ggaacccaga
     1561 agccgggcgc agccaggaca ggcgaagagg cgcgtgacct gcgctcttcc tcgtcgggcc
     1621 ggccggattc gaaccggcga cctcttgacc cccagtcaag cgcgctaacc aagctgcgcc
     1681 acggcccgca ccgttcagcg gcgagtctag caggggcgcc ggttcggagg atcggcggcg
     1741 ggtcgagggg caggcgtatc tgagggccgc cgcccggacg ccccgcgcga ccatcgcggg
     1801 tcggctaggc tccggttcca accgagtccg gaggatcttg gatgtacgtc tgcctatgca
     1861 tggggatcga cgatcgggcc gcgcgggaag cgatccgagc cggcgcctcg accgtccgcg
     1921 acgtcctgcg cgcgtgcggg agcgcgcgcc cgtgcggcgg atgcagcccc gtcatccgag
     1981 tcctcctgca ccaggcgtcc gccgccgacg aaaccagcgt gatccgcatc gaccagtacc
     2041 gggaggcacg atgaagggca acgaacgagt catcgagacg ctgaacgagc tgctcaccct
     2101 cgagctcacc gtgatcaatc agtacttcct gcactcgaag atgtgcgagg actggggata
     2161 cgcccggctc gcggcgaagc tccgggccgt ctcccgcgag gagatggacg acgccgaaga
     2221 gatcatcgac cgggtcctct tcctggatgg ggtgccgaac atgcagcgcc tcgggcccgt
     2281 cacgatcggc cagacggtgc ccgagcagct ccggatccac ctcgacgccg agcggcgcgc
     2341 gctcgagctg ctgagccagg gcatcggcgt cgccgacgac gccggggacg ccgcgtcacg
     2401 cgagttcttc gcctcacgcc tgcaggagga gga
//
LOCUS       DATLGG010000304         3669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_156065, whole genome shotgun sequence.
ACCESSION   DATLGG010000304 DATLGG010000000
VERSION     DATLGG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156065"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VM638_08620"
     CDS             complement(<1..895)
                     /locus_tag="VM638_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVM12522.1"
                     /translation="MKTSMHGHPGHHADPGHGPSVARSDGHERHAGHSAEMFRSRFWV
                     SLVLTIPVLAYADVLWEVVGLRPPQPPGSRWVPFLLATAIYVYGGSVFLRSSLGELRA
                     RRPGMMTLVSLGISVAYLYSATVTILFAESDGFYWELATLVTIMLLGHWVEMRSIGRA
                     SAALSELARLLPDSAERLGEHGPETVPIQALEEGDLVLVRPGARVPADGEVEEGRSSV
                     DESMLTGESRPVPKSPGSPVVAGTVNGEGALHVRVTGTGERTALAGIMRLVEEAQASR
                     SRSQDVADRAAMWLFTIAVIAG"
     gene            complement(941..1013)
                     /locus_tag="VM638_08625"
     tRNA            complement(941..1013)
                     /locus_tag="VM638_08625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(978..980),aa:Ala,seq:tgc)
     gene            1217..1675
                     /locus_tag="VM638_08630"
     CDS             1217..1675
                     /locus_tag="VM638_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12523.1"
                     /translation="MIDYAVDATWRPARLRIQTQEHRLVLVADAGRLVGERDGEPLEI
                     PYREDLGYRSPVFNAVTANRLGRSGDVEALSLETETLRDRPERQRYELQGPERVATPV
                     GAFEATVWRYTALETGWTARLCVAGDVVVAFEGAYELISYEPGPRGPFPS"
     gene            1672..2535
                     /locus_tag="VM638_08635"
     CDS             1672..2535
                     /locus_tag="VM638_08635"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVM12524.1"
                     /translation="MSGYLIVANPGAGSGDRSPAAEARRRLADARSLTLEQGVDLKAA
                     IDEAVAEERIVVAAGGDGTIHAVAQHLVHRGTLGILPMGTVNNFARDLGVGRAEVALA
                     ALEDPRPRQIDVGRVGSRIFVNNLSVGLYPELVRHRERIEDDVGRWAAAAAAAYRVLR
                     RGRPLTGIATADGDRRLLSAWVLFVGNNRFRASTTRPGVRDRLDEGVLDVRVITARPG
                     SRRARAAWRILRGRDWPLRRQVRTPARRLQVILDDARAIAFDGEMGARAARFTVETVP
                     GALRVLGAPDD"
     gene            complement(2532..2684)
                     /locus_tag="VM638_08640"
     CDS             complement(2532..2684)
                     /locus_tag="VM638_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12525.1"
                     /translation="MGEEKKGKKRFFLWAMLVAIGAAVVAVFRRRRSQDLEESEWQEL
                     PPADGS"
     gene            complement(2713..2786)
                     /locus_tag="VM638_08645"
     tRNA            complement(2713..2786)
                     /locus_tag="VM638_08645"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2750..2752),aa:Ile,seq:gat)
     gene            complement(2835..3236)
                     /locus_tag="VM638_08650"
     CDS             complement(2835..3236)
                     /locus_tag="VM638_08650"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HVM12526.1"
                     /translation="MATVQTKPAAAAAKRGGPRRIRVTVRKVNPWSVLKLSLVFYFCL
                     LLIVLSGLAILFMLLDAMGLIDSIAGLLTGLTILEPSAEIRMFPVFRVLFLIGFITVI
                     VGSVFTMFLAFLYNLLSDLVGGIEVTLLERR"
     gene            complement(3348..3548)
                     /locus_tag="VM638_08655"
     CDS             complement(3348..3548)
                     /locus_tag="VM638_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12527.1"
                     /translation="MAALLLRPRAPVALSVGLLALSGAGVAWGGILLAGGSGLELAVL
                     APLLALLVPFHVRVVLGPFRRR"
BASE COUNT          571 a         1291 c         1337 g          470 t
ORIGIN      
        1 caccggcgat gacggcgatc gtgaagagcc acatcgcggc gcggtcggcg acgtcctggg
       61 agcgggaccg ggaggcttgc gcttcttcga cgagacgcat gatccccgcg agcgcggtcc
      121 gctcaccggt cccggtgacg cgcacgtgaa gggccccctc cccgttcacc gtgccggcga
      181 cgacggggga gcccggagac ttcgggacgg gcctggactc cccggtcagc atggactcgt
      241 cgaccgacga tcggccctcc tcgacctctc cgtcggcggg cacgcgggcc cccggccgca
      301 cgaggaccag gtcgccctcc tccagcgcct ggatcgggac ggtctcgggg ccgtgttcgc
      361 cgaggcgctc cgcgctgtcc ggcaggagcc gtgccagctc cgagagggcc gcgctcgccc
      421 ggccgatgga gcgcatctcg acccaatggc ccagcagcat gatcgtcacc agcgtcgcga
      481 gctcccagta gaagccgtcc gactcggcga agaggatcgt gaccgtcgcg ctgtagaggt
      541 aggcgaccga gatgcccagc gagacgagcg tcatcatgcc cgggcgacgt gcccgcagct
      601 cgccgagcga cgaacggagg aacacgctgc ccccgtagac gtagatcgcc gtggcgagga
      661 ggaacggcac ccagcgcgag ccaggaggtt gcggcggccg gagaccgacg acctcccaca
      721 gcacgtccgc gtacgcgagg accgggatgg tgagcacgag gctcacccag aaccggctcc
      781 ggaacatctc ggccgagtgg ccggcgtgcc gttcgtgtcc gtccgatcgg gcgacggatg
      841 gtccgtggcc gggatcggcg tggtgcccgg ggtgcccgtg catggacgtc ttcatggccc
      901 gcacgatata cccccggggg gtatccgtca acctgctgcg tggagacgac cggattcgaa
      961 ccggcgaccc ccggcttgca aagccggtgc tctaccagct gagctacgtc ccctccgccc
     1021 atgataggtg ctggcagctc cgggttggcc cctcgacccg ggggtagcct tcggggatgc
     1081 cggtgggggt ctatcgcgtg cggggcgagg gggatcgggc agatacacac tggaggagtt
     1141 ccgctgcgcc ccaggtccgg caggatggcg gtggttctcc accgtctcat ccgagggacc
     1201 cgacccgcgc agcgaggtga tcgattacgc ggtcgacgcg acctggcgcc cggcgcggct
     1261 ccggatccag acgcaggagc accgcctcgt cctcgtcgcc gacgccggcc gcctcgtggg
     1321 ggagcgcgac ggtgagcccc tcgagatccc ctaccgcgag gacctcggct accgctcgcc
     1381 cgtgttcaac gcagtgaccg cgaaccgcct ggggcgcagc ggcgacgtcg aagcgctctc
     1441 cctggagacc gagacgctgc gggaccgccc ggagcgtcag cggtacgagc tccaggggcc
     1501 ggagcgcgtg gcgacgcccg tcggcgcgtt cgaggcgacc gtctggcgct acacggccct
     1561 cgagaccggg tggacggccc gcctctgcgt cgccggagat gtggtcgtgg ccttcgaggg
     1621 agcgtacgag ctcatctcgt acgagcctgg gccgagaggg cctttcccgt cgtgagcggc
     1681 tacctgatcg tcgcgaaccc cggcgccggc agcggcgatc gctccccggc ggcggaggcc
     1741 cgtcgacggc tcgcggatgc gcgcagcctg acgctcgagc agggtgtgga cctgaaggcc
     1801 gcgatcgacg aggccgtcgc cgaggagcgg atcgtggtgg cggccggggg ggacgggacg
     1861 atccacgccg tcgcgcagca cctcgtccac cgcgggacgc tcgggatcct gccgatgggc
     1921 acggtgaaca acttcgctcg ggacctgggg gtcgggaggg cggaggtcgc gctcgcggcc
     1981 ctcgaagatc ctcgcccgcg ccagatcgac gtcggacgtg tcgggagccg catcttcgtc
     2041 aacaacctct cggtcggcct ctaccccgag ctcgtgcgcc accgcgagcg gatcgaggac
     2101 gacgttggtc gctgggcggc ggcggcggcg gcggcgtacc gggtgctgcg tcgcggacgc
     2161 ccgctgaccg gcatcgcgac agcggacggg gatcgccgcc tgctctccgc gtgggtcctg
     2221 ttcgtgggga acaaccgctt ccgtgcctca accaccaggc ccggggtgcg ggatcgtctc
     2281 gacgagggcg tcctggacgt ccgggtgatc accgcgcggc cgggaagccg ccgggcgcgg
     2341 gccgcgtggc gcatccttcg cgggagggat tggcccctgc ggcgtcaggt ccgcacgccg
     2401 gcgcggcgtt tgcaggtcat cctcgacgac gcgcgcgcga tcgccttcga cggcgagatg
     2461 ggcgcccgcg cggcccggtt cacggtcgag accgtgccgg gcgcgcttcg cgtgctaggg
     2521 gcgccggacg actagctgcc gtcggccgga gggagctcct gccactcgct ctcctccagg
     2581 tcctgcgagc ggcgacgacg gaacaccgcc acgacggcag cgccgatcgc gacgagcatc
     2641 gcccacagga agaaccgctt cttgcccttc ttctcctcgc ccatgtctgt ctcctcctcg
     2701 tggtcgcgtg cgtgggcctg ggtggactcg aaccaccgac ctcaccctta tcaggggtgc
     2761 gctctaaccg cctgagctac aggcccgaga gcggcctaca ccgtaccgcc gccccgcgga
     2821 gaccgtcaaa cgggtcagcg ccgctcgagc agcgtgacct cgatcccgcc gaccaggtcg
     2881 gagagcaggt tgtacaggaa ggcgaggaac atcgtgaaga cggacccgac gatcacggtg
     2941 atgaagccga tcaggaagag cacccggaag acggggaaca tccggatctc ggccgagggc
     3001 tcgaggatcg tcaggcccgt gagcaggccg gcgatcgaat cgatcaggcc catcgcgtcg
     3061 aggagcatga acaggatcgc cagccccgag aggacgatca gcaggaggca gaagtagaag
     3121 acgagcgaaa gcttcaggac cgaccagggg ttgaccttcc gtaccgtcac ccggatccgt
     3181 cggggccccc cgcgtttcgc cgccgcggcg gccggcttcg tctgaacggt tgccatcgct
     3241 gtccttccag caggatcggc cccacccgag gccaaggaga gtctagcccg gcggatgagc
     3301 gcggcggccg aagcccgggg aggaatcgcc tcgggcaggg aggggcgtca gcgccggcgg
     3361 aacggcccga ggacgacccg gacgtggaac ggaacgagca gcgccagcag cggagcgagc
     3421 accgccagct ccaacccgga accgcccgcg agcaggatcc cgccccaggc gacaccggca
     3481 cccgacagcg cgaggaggcc gacgctcagc gcgaccgggg cacgggggcg caggaggagc
     3541 gccgccacga gcgccagccc ggctgccgca gcgacgatga gcgcgccgcc ccccaggtca
     3601 cgcctcctcg ccggcctgga cgatgacggg cgccaccgag gctaccttct cgcccgagcg
     3661 gagacgcat
//
LOCUS       DATLGG010000305         4884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_218215, whole genome shotgun sequence.
ACCESSION   DATLGG010000305 DATLGG010000000
VERSION     DATLGG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218215"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VM638_08660"
     CDS             complement(<1..256)
                     /locus_tag="VM638_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12528.1"
                     /translation="MAWRGRVALFALFAVMLIPLGTSTLRGLSHVLICAEDVATPFTL
                     TVEPGEEPTVTSSQTIERDAPPASGVAPTPSPSAGRLCGGL"
     gene            334..1188
                     /locus_tag="VM638_08665"
     CDS             334..1188
                     /locus_tag="VM638_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12529.1"
                     /translation="MRGGLRGLGPRGIRWLILTAGVLVLLLIAAVVYVRRVEPVEVIG
                     TLLFIPVFLALTLWGWRGGLALGLLAAATYTWLRAPAIEAVGLDRFIGLIVSRTIAYV
                     AFGAIGGWAARQLQLSLTKLELYDQIDDATGLYNARFFLEQTELEDSRAKRYRTMYSV
                     ASVDVPQNALERLRGRRRVRALRDLGRMIRTSVRTVDRAVHADDGIYHRFTVILPETG
                     REGARVFAGRLAERVAAHYTERGADLQADRLQLTVATGPEDEEELRRLRDEYAAINRA
                     EHPDPPGS"
     gene            1368..2183
                     /locus_tag="VM638_08670"
     CDS             1368..2183
                     /locus_tag="VM638_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12530.1"
                     /translation="MVHIEMSDTDMSRAVEELWNPQQEESAGTREELEQRVEDRTAEL
                     AAAVEELESFAYSVSHDLRAPLRAITGFSRILLEEHADHLDAEGRRMLEIIDANVSRM
                     GQLIDGLLRFSRLGRAEMERKPVDLTALVGSVVDQLALEDPDRDVKVVIRPLGVARCD
                     HLTVRQVLANLIGNAWKFTRHEPDPVIEVGRRTEDGQDLYYVSDNGVGFDERYAAKLF
                     GMFQRLHRDDEFEGHGIGLAIANRVIQRHGGRLWAVGRIGAGAEFRFTLGEAA"
     gene            2180..2608
                     /locus_tag="VM638_08675"
     CDS             2180..2608
                     /locus_tag="VM638_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM12531.1"
                     /translation="MKPHETVEVLLVEDNPADAELTIRALQRNNLTNNVVVARDGAEA
                     LDLLFGSNPLPVRPRLILLDLKLPRVDGLAVLRHLKSDPRTKTIPVVVLTSSDHDRDV
                     VESHQLGVNSYIVKPVEFDKFVRSVAEVGMYWLLLNRVPA"
     gene            2605..>4884
                     /locus_tag="VM638_08680"
     CDS             2605..>4884
                     /locus_tag="VM638_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVM12532.1"
                     /translation="MSRHAPTPGPRLRLLYLEDDRTDLELLDAELRRSSMLYELRHVD
                     DHARFVRELERFEPHAVLADYQLPSFDGMTALQIVLERSPETPFIFVSGALGDARATE
                     TLKMGAADYVLKDRLHRLVPAIHGAVERSRERTARHEAEEMYRTLFDHVPIGLFRSAP
                     DGRFIDVNRAAVEMFGFADAREMLACDPTALFADPTEHDQIRARLEVDGALRDHEFEA
                     RRRDGTLLWVRSSARAVLAGGSLRYAEGTMEDITAERHNRDRLEESEERHRIISSLVS
                     DYAYSIAIEPDASLRLEWVTEAFQRQFGVTVEEANARGLMEFVHPDDRERAMAALGRV
                     LAGEHADIEYRMLARDGSTRWVRSRIRARRDPLTDGVVRLVGSSEDVTERKEADLALL
                     RSEGRLRDAQAVAHVGSWEWDPETDEVQWSDELYRILGYAPGEVAASRQLYIQMVHPE
                     DRAGVIAEVEQAKSKDSVSYHHRIVRGDGTVAHVSTQGRRLRSTQGGGWRLLGTVADV
                     TEMELARQALEEKALQQQVVADLARKACEDIALDDLYQEAARAVATTLSLDMVSMFEL
                     LPNGQELFLRGGYGWQGDYVGSVTVPADERTQAWQAIRLGAPVVSVDIAADPRFEPLW
                     FRSENGVISGAVVVMQAGGEPFGAIVADSVTPRMFSEREVTFLQMVANILAGFIRRAE
                     AEHALRVSEQRYRSLFEGVPVGLYRSTRDGRILEANPAMAQILGFDSSEALLQGRAES
                     VYLDPADREHWVEQLEERGE"
BASE COUNT          785 a         1642 c         1728 g          729 t
ORIGIN      
        1 agaggccgcc acagaggcgg cctgccgacg gggagggcgt cggcgccacc ccggacgcgg
       61 gcggtgcgtc tcgctcgatc gtctgcgagg aggtcaccgt cggctcttcc ccgggctcga
      121 ccgtgagcgt gaagggcgtc gccacgtcct cggcgcagat gagcacgtgc gagagccccc
      181 gcagggtcga cgtcccgagc gggatcagca tcaccgcgaa caaggcgaac agcgccaccc
      241 gtccccgcca ggccatgtct ccagtgtcgg gtgacggggc ggagtggtca agcggtcgtg
      301 gaccccacac acgggctcgc gtagcatgca gccatgcgag gtggcctccg aggcctcggg
      361 ccccgcggga tccggtggct gatcctcacc gccggggtcc tcgtcctgct cttgatcgcc
      421 gcggtcgtct acgtgcggcg ggtcgagccc gtcgaggtga tcggaaccct cctgttcatc
      481 ccggtcttcc tcgcgttgac cctctggggg tggcggggag ggctcgcctt ggggctcctt
      541 gccgccgcga cctacacgtg gctccgcgca cccgccatcg aggcggtggg cctcgaccgg
      601 ttcatcgggc tgatcgtttc ccgcacgatc gcctacgtcg cgttcggggc gatcggcggc
      661 tgggcggccc gacagctgca gctctcgctc acgaagctcg agctgtacga ccagatcgac
      721 gacgcgaccg gcctctacaa cgcgcgcttc ttcctcgagc aaaccgagct cgaggactcc
      781 cgcgcgaagc ggtaccggac gatgtactcg gtggcctccg tcgacgtccc acagaacgct
      841 ctggagcggc tccgcgggag gagacgcgtc cgggccctgc gggacctcgg tcgcatgatc
      901 cgcacgagcg tgcggacggt cgaccgcgcc gtccacgccg acgacgggat ctaccaccgg
      961 ttcaccgtga tccttccgga aacgggccgc gagggtgcac gggtgttcgc cggtcgtctc
     1021 gcagagcgcg tcgccgccca ctacaccgag cgcggtgcgg acctgcaggc ggaccggctg
     1081 cagctcaccg tcgcgaccgg ccccgaggac gaagaggagc tccgccggct cagggacgag
     1141 tacgccgcga tcaaccgcgc ggaacatccg gatccgcccg gttcgtgacc ggccctgaag
     1201 gggcatcccg cggctggggg acagggcccc gacgatcggc ccacacgggt ggggggccac
     1261 tcgtcatctc agatctgagg ccttctggcc attcatttct acaacgggct cttcgacaat
     1321 gcccttagga gcggatgctc ccgcaacgcc caggtcaagg gaaggacgtg gtccacatcg
     1381 agatgtcgga caccgacatg agcagggcgg tcgaagagct ttggaacccg cagcaggagg
     1441 agtccgccgg aacccgcgaa gagctggagc agcgggtgga ggatcggacc gccgagctcg
     1501 ccgccgcggt cgaggagctc gagtccttcg cctactccgt ttcgcacgac ctgcgggcgc
     1561 ccctgcgggc catcaccggc ttctcccgga tcctgctcga ggagcacgcg gatcatctgg
     1621 acgccgaagg caggcggatg ctcgagatca tcgacgcgaa cgtcagccgg atggggcagc
     1681 tgatcgacgg actgctgcgc ttctcgcggc tcggccgcgc ggagatggaa cgtaagccgg
     1741 tggacctcac cgcgctggtg ggctccgtcg tcgaccagct ggccctcgag gatccggacc
     1801 gcgacgtgaa ggtcgtcatc cgtccgctcg gcgtggcgcg ctgcgaccat ctcacggtgc
     1861 gccaggtgct ggcgaacctc atcggcaacg cgtggaagtt cacccgccat gagccggatc
     1921 cggtgatcga ggtcggccgc cgcaccgagg atggccagga cctctactac gtaagcgaca
     1981 acggggtcgg gttcgacgag cggtacgccg ccaagctgtt cgggatgttc cagcgactcc
     2041 accgcgatga cgagttcgag ggacacggca tcggtctggc gatcgcgaac cgtgtgatcc
     2101 aacgtcacgg aggccggctc tgggccgtgg gacggatcgg tgccggcgcc gagttccgct
     2161 tcaccctcgg ggaggcagca tgaagccgca cgagacggtg gaggtcctgc tcgtcgagga
     2221 caacccggcg gacgcggagc tcacgatccg cgccctccaa aggaacaacc tcaccaacaa
     2281 cgtggtggtg gcgagggatg gagccgaagc actggacctg ctgttcgggt ccaaccccct
     2341 gcccgtacgg ccccggctga tcctgctgga cctcaagctg ccgcgcgtgg acggcctcgc
     2401 cgttctccgc catctgaaga gcgatccacg gacgaagacc atccccgtcg tcgtcctcac
     2461 ctcgtccgac cacgaccgcg acgtcgtgga gagccaccag ctcggcgtga acagctacat
     2521 cgtcaaaccg gtggagttcg acaagttcgt ccgctcggtg gccgaggtcg gcatgtactg
     2581 gctgctgctg aatcgggtgc cggcatgagc aggcacgcgc cgacgccggg tccgcgtctg
     2641 cgtctcctct acctggagga cgaccgcacg gacctcgagc ttctcgatgc cgagctgcgt
     2701 cgcagcagca tgctctacga gctccgccac gtggatgacc acgcgcgctt cgtgcgggag
     2761 ctggagcgct tcgagcccca cgccgtcctc gccgactacc agcttccgtc gttcgatggg
     2821 atgacggcgc tccagatcgt cctcgagcga tcgccggaga cgccgttcat cttcgtgagc
     2881 ggcgcgctgg gagacgcgcg ggcgacggaa acgctgaaga tgggcgcagc tgactacgtg
     2941 ctgaaagacc gcctccaccg gctcgttccg gcgatccacg gcgccgtcga acgctcgcgg
     3001 gagcgcacgg cgcggcacga ggcggaggag atgtaccgca cgctcttcga ccacgtcccg
     3061 atcggactct tccgctcggc gccggacggc cgcttcatcg acgtgaaccg cgcggccgtc
     3121 gagatgttcg ggttcgcgga cgcgcgcgag atgctcgcgt gcgatcccac ggccttgttc
     3181 gccgacccta ccgagcacga ccagatccga gcgcggctcg aggtcgatgg cgccctccgc
     3241 gaccacgagt tcgaagcacg gcgcagggat gggaccctct tgtgggttcg gtcctccgcg
     3301 cgggccgttc tcgccggcgg aagcctgcgg tacgccgagg gaacgatgga ggacatcacg
     3361 gccgagcgac acaaccgcga tcgcctcgag gagagcgagg agcggcatcg catcatcagc
     3421 agcctcgtct ccgactacgc gtactcgatc gcgatcgagc cggacgcttc cctgcggctc
     3481 gaatgggtca ccgaggcgtt ccagcggcag ttcggcgtta ccgtggaaga ggcgaacgcg
     3541 cgtggcctca tggagttcgt ccaccccgac gaccgcgaac gcgcgatggc ggcactcggc
     3601 cgcgtccttg ccggcgagca cgccgacatc gagtaccgga tgctggccag ggacggcagt
     3661 acgaggtggg ttcgctcccg gatccgcgcc cgtcgcgacc cgctcacgga cggggtcgtg
     3721 cggctggtgg gctcctccga ggacgtgacg gagcgcaagg aggcggatct ggcgctgctg
     3781 cgcagcgagg gccgcctccg ggacgcgcag gccgtcgcgc acgtcggcag ctgggaatgg
     3841 gacccggaga ccgacgaggt ccagtggtcc gatgagctct accgtatcct cgggtacgcc
     3901 ccgggcgagg tggcggcgag ccgacagctg tacatccaga tggtccaccc cgaggaccgg
     3961 gcaggggtga tcgccgaagt ggagcaggcg aagtcgaagg attcggtcag ctaccaccac
     4021 cggatcgttc ggggcgacgg cacggtcgcg cacgtgagca cgcaggggcg tcggctccgg
     4081 tcgacgcagg gtggtggatg gcgcctcctg ggcaccgtcg cggacgtcac ggagatggag
     4141 ctcgcccgcc aggctctcga ggagaaggcc ctgcagcagc aggtcgtggc cgacctggcc
     4201 cggaaagcgt gcgaggatat cgcgctcgac gacctctacc aggaagcggc acgggcggtc
     4261 gcaacgacgc tcagcctcga catggtgagc atgttcgagc tcctcccgaa cggccaagag
     4321 ctcttcctgc gaggcggcta cggatggcag ggtgactacg tcggatccgt gacggtccca
     4381 gcggatgagc ggacgcaggc gtggcaggcg atccgcctcg gtgcgccggt cgtctcggtc
     4441 gacatcgcgg ccgatcctcg gttcgagcct ctgtggttcc gctcggagaa cggcgtcatc
     4501 agcggggcgg tcgtcgtgat gcaggcgggt ggcgagccgt tcggcgcgat cgtcgcagat
     4561 tcggtcaccc cgcggatgtt ctcggagcgg gaggtgacct tcctccagat ggtggcgaac
     4621 atcctggcgg gcttcatccg gcgtgccgag gccgaacacg ccctgcgggt gagcgagcaa
     4681 cggtaccgga gcctgttcga gggggtaccg gtgggcctgt accgatcgac gcgggacggg
     4741 cggatcctgg aggcgaaccc ggcgatggcc cagatcctcg gcttcgactc cagcgaagca
     4801 cttctgcagg gacgggcgga gtccgtctac ctcgatcccg ccgaccgaga gcactgggtc
     4861 gagcagctcg aggagcgcgg ggag
//
LOCUS       DATLGG010000306         3115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_202750, whole genome shotgun sequence.
ACCESSION   DATLGG010000306 DATLGG010000000
VERSION     DATLGG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202750"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1232
                     /locus_tag="VM638_08685"
     CDS             51..1232
                     /locus_tag="VM638_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM12533.1"
                     /translation="MRQLYLAATGQNRGKTTAALGLLEAFLARGLRTGFMKPVGQRTV
                     IEDGVPADEDAVLMKSVFGLPETYRDMSPVHIPRGFTKAYIGGRVVDDLGGRIREAHR
                     AFDGHDMVLIEGTGHAGVGAVIGLSNATVAGMLGAPAIIVSEGGVGRPIDEIVLNKAL
                     FVSHGVTVAGAIVNKVDLDAQPGLPAVLARGLAPYGIPLLGVLPVRPLLSNPTLGMIL
                     EGLHGETIHPGPDLDRVIDGVAIGAMEPAHMLDRVGPGTLVIVPGDREDVILTITTSH
                     LARRVRLANATEAARIAAIAAEATNERTASVAGHEGAAIGLLLTGGYRPREAVLAAIR
                     QADLFATLVTDDTYAVASQVHDLLVKTHAGDRDKVAVIKSLVEGNLDVDRILAAAAPG
                     L"
     gene            1660..2145
                     /locus_tag="VM638_08690"
     CDS             1660..2145
                     /locus_tag="VM638_08690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVM12534.1"
                     /translation="MKTRILRALALALTVAIALPAITSAAWPVTSHSSYVSQKFHDGH
                     RGLDIAATKWTGVVPIGNGKVVFAGWKNNCGGYQVWVRHRDGVTHSAYYHLAQETTYS
                     GHYVTGEKTRIGYVGTTGCVTGAHVHVEVWKGTPWRSGSYRVNPWNYVKKGYWLPYQY
                     R"
     gene            complement(2209..3051)
                     /gene="panC"
                     /locus_tag="VM638_08695"
     CDS             complement(2209..3051)
                     /gene="panC"
                     /locus_tag="VM638_08695"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HVM12535.1"
                     /translation="MTQLLRTRAELRHALTAAARPVGLVPTMGWLHGGHRALMRRSRE
                     ENGTSVVTIFVNPRQFNEASDYTRYPRNEAQDIAICDEEGADLVWAPPVEEVYPPGFD
                     TTVSVGALARPLEGAARPGHFDGVATVVAILFSLVGAERAYFGQKDAQQVMVIRQMAR
                     DLAIGTEVVACPTVREPDGLALSSRNVHLTAEQRAAAPVVRRALVAARQRWETGERRA
                     EALRDVMWEVLTAEPLASPEYVSVADGRTLEELDRIDGPVLLSMAVGFGQTRLIDNEP
                     LNGP"
BASE COUNT          459 a         1143 c         1061 g          452 t
ORIGIN      
        1 gctgggggcc acgagagcgg ctgccggctc ggcgctgacg gtaccgacgc atgagacagc
       61 tctacctggc ggctacgggg cagaaccggg gcaagacgac ggccgccctg gggctgctgg
      121 aggccttcct cgcccgcggc ctgcgcacgg gtttcatgaa gccggtcggg cagcggaccg
      181 tcatcgagga cggcgtcccg gccgacgagg acgcggtcct gatgaaaagc gtcttcggtc
      241 tcccggagac gtatcgcgac atgagcccgg tccacatccc gcggggcttc accaaggcct
      301 acatcggtgg ccgcgtcgtc gacgacctgg gtggacgcat ccgcgaggcg caccgtgcgt
      361 tcgacgggca cgacatggtc ctcatcgagg gcacgggcca cgccggggtc ggggccgtga
      421 tcgggctgtc caatgcgacg gtggccggga tgctcggcgc cccggcgatc atcgtgtcgg
      481 agggcggtgt cggacgaccg atcgacgaga tcgtcctcaa taaggcgctc ttcgtcagcc
      541 acggggtgac ggtcgcggga gcaatcgtca acaaggtcga tctcgacgcc cagccgggcc
      601 tacccgcggt cctcgcgcgc ggcctggcgc cgtacgggat cccgctgctc ggcgtgctgc
      661 cggtcaggcc gctcctgtcc aaccccacgc tcggcatgat cctcgagggg ctgcacggcg
      721 agacgatcca tcccggcccc gatctcgacc gcgtgatcga cggtgtggcg atcggtgcga
      781 tggagccggc acacatgctc gaccgggtcg ggccgggcac gctcgtgatc gtgccggggg
      841 atcgggagga cgtcatcctc acgatcacga cgtcccatct cgcccgacgg gtccgcctgg
      901 ccaacgccac cgaggccgcc cggatcgccg ccatcgcggc cgaggcgacg aacgagcgga
      961 cggcgtccgt ggccggccac gagggagcgg ccatcggcct gctgctgacc ggggggtacc
     1021 gtccccgcga agcggtcctc gcggcgatcc ggcaggcgga tctcttcgcc accctcgtca
     1081 ccgatgacac gtacgccgtc gcttcgcagg tccacgacct gctcgtgaag acccacgccg
     1141 gcgatcggga caaggtcgcc gtgatcaagt ccctggtcga gggcaacctg gacgtcgacc
     1201 ggatcctcgc tgcggccgcc cccggcctgt gacgcgggcg ttgcccgggt gcagcgccca
     1261 tcggcccacg ggcgtgtggc ggggttcctc gctgttccga tctgtagtag cgttgcaatc
     1321 cgtcaggcgc ggatggaacc gggtctggca ggtcgggaac ctcaccggtc gggtcggcac
     1381 aggcccccca gctccgccgc ccgctccgtt ccgacccgcc gacgccgcgc ccccagcgcg
     1441 cgcagaggca ttgccaccgc acgtccacga ggcgtgcccg cccacgcacc ggttcggttc
     1501 tggccgctcg cgcgcgtgcc ttcgccgccg tgcccaagga cgccccccat cggtggggct
     1561 cgagaccgcc gtccatccac cgctggcccc ggttagtccc gtgtggcctc caggcagaaa
     1621 ggagcagggg tagcgctccg tgcgtgccca ccagccttca tgaagaccag gatccttcgc
     1681 gctctcgctc ttgcgctcac cgttgccatc gcgctcccgg caatcacgtc ggccgcctgg
     1741 cccgtgacca gccactcctc gtacgtcagc cagaagttcc acgacggcca ccgcggtctc
     1801 gacatcgcgg ccacgaagtg gacgggcgtc gtcccgatcg gcaacggcaa ggtcgtgttc
     1861 gccggctgga agaacaactg cggcgggtac caggtctggg tgcgccaccg tgatggcgtc
     1921 acgcacagcg cctactacca cctggcccag gagaccacgt actccgggca ctacgtgacg
     1981 ggggagaaga cccggatcgg gtacgtcggc accaccggct gcgtcaccgg ggcgcacgtc
     2041 cacgtcgagg tgtggaaagg cacgccctgg cgtagtggct cctaccgggt caatccctgg
     2101 aactacgtca agaagggcta ctggctcccg taccagtacc gctgatccgg aaccccaccg
     2161 gcgaacgtcg atcggcgctc cggctcaccc ggagcgccga tttcgcgctc acggcccatt
     2221 gagcggctcg ttgtcgatga gccgggtctg gccgaacccg accgccatcg acaggagcac
     2281 cgggccgtcg atccgatcca gctcctcgag cgtccgcccg tccgccaccg acacgtactc
     2341 aggcgatgcc agcggctccg cggtgagcac ttcccacatg acatcgcgga gcgcctcggc
     2401 gcgtcgctca cccgtctccc atcgttgccg ggccgcgacg agcgcgcgac ggacgaccgg
     2461 cgccgcagcg cgctgctcgg ccgtgaggtg gacgttgcgc gacgagagcg caagtccgtc
     2521 gggttcccgc accgtgggac aggcgacgac ctccgtgccg attgcgaggt cccgcgccat
     2581 ctgccggatg accatcacct gctgggcgtc cttctgcccg aagtaggccc gctccgcgcc
     2641 gacgagcgag aacaggatcg caacgacggt ggcgaccccg tcgaagtggc cgggacgcgc
     2701 cgcgccctcg agcggtcggg cgagcgcccc gaccgacacg gtcgtgtcga agcccggcgg
     2761 gtagacctcc tcgaccggcg gcgcccagac gagatcggcg ccctcctcat cgcagatcgc
     2821 gatgtcctgc gcctcgttcc gcgggtacct ggtgtagtcg ctcgcctcgt tgaactggcg
     2881 cgggttcacg aagatcgtca cgacggacgt cccgttctcc tcgcgactcc gtcgcatgag
     2941 cgcgcgatga cctccgtgca gccagcccat cgtcggaacg agccccaccg gccgggcggc
     3001 cgcggtcagc gcgtgccgca actccgcgcg cgttcggagc agctgggtca cgtcagccta
     3061 gaggtcgcgg tcgagcggga tcccgccggc gatctcgtcc ggttccccgg cgccg
//
LOCUS       DATLGG010000307         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_185593, whole genome shotgun sequence.
ACCESSION   DATLGG010000307 DATLGG010000000
VERSION     DATLGG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185593"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VM638_08700"
     CDS             <1..972
                     /locus_tag="VM638_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVM12536.1"
                     /translation="HCLAKYVSPDHPDHASLAREPLAEKIRDVYRLLDDGLGRHLAHA
                     RDDDLVLFVSDHGFRSCARALHMDRVLEQLGFLRFTASRAVFGPMQWGPMRAAARRVY
                     DLLGLHGRVSLPQSVNREKTVAYTSVRSTGEGVSINLAGREPDGIVDPADYGTTRDRV
                     MDALSSFRDPATGASPFAAIRRREDVFQGRHVETAPDIVLEPAPLYSLTHARSAVEDA
                     DWLSGDHRMEGVIAAAGPHVRADAFDEPAMLIDVAPTVLAALGVPPSVKHDGQVLTAV
                     VGAEAQAETTVAPPPRPQGSDVEDPSGLDEEEARQVEDHLRGLGYLE"
     gene            956..>2346
                     /locus_tag="VM638_08705"
     CDS             956..>2346
                     /locus_tag="VM638_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVM12537.1"
                     /translation="MATSSSEPGPRPRGIRALIEAVLPAARFGDERAIAAGTGQNLVG
                     LVVAMLATFATQVLITRVLGAAAFGAVTVATQLAFVGAAATRFGMDMASVRLVAIHAG
                     RGEREVLRGVVRRAAGIAALVSVVVGAGIFAGADALARTFASPGEGGAFRAAAIALPF
                     AALVHVYLGATRGLKVMRHTLTVYWVGQPLLWILLLLSGWLVARSIETSVAAYAGSWA
                     IATVAALLLWRRETPGPEAPVPAGEMRQLVRYGAPRAPAALSAQLLFWTDLFVLARVA
                     PAAEVGVYAAAVRAGQVILLFLISVNNMFSPFVADLHARGERQRLDALYKLVTRWMLV
                     ATLPILIVLAVVPDAVLHLFGEGFGRGRTALLILLGGQLVNVATGSVGFILIMVGRTG
                     WDLVVNGVAVAFSLGVAMLLAPRYGMEGAAVAGATTLALGNLWRLALVRRLVGIQPFT
                     RDHLRLLPPALAGA"
BASE COUNT          267 a          854 c          862 g          363 t
ORIGIN      
        1 cattgcctgg cgaagtacgt gtccccggat cacccggatc atgccagcct cgcccgcgag
       61 ccgctcgccg agaagatccg cgacgtctac cgcctgctcg acgacgggct cggccgccat
      121 ctcgcgcacg cgcgcgacga cgacctcgtg ttgttcgtct cggaccacgg gttccgctcc
      181 tgcgcgcgcg ccctgcacat ggaccgggtg ctcgagcagc tcgggttcct ccgcttcacg
      241 gccagccgtg ccgtcttcgg gccgatgcaa tggggtccga tgcgggcggc ggctcggcgc
      301 gtctacgacc tgctcggcct ccacgggcgc gtctcgctcc cgcagtccgt gaaccgcgag
      361 aagaccgtcg cttacacgtc ggtgcgctcg accggcgagg gcgtgtcgat caacctcgcg
      421 ggccgcgagc ccgacggcat cgtggaccct gccgactacg gaacgacccg ggaccgcgtg
      481 atggacgcgc tctcgagctt ccgcgacccg gcgaccgggg cgagcccgtt cgccgcgatc
      541 cggcgccgcg aggacgtctt ccagggccgg cacgtcgaga cggcgccgga catcgtcctc
      601 gagcccgcac ccctctacag cctcacgcat gcgcgctcgg ccgtcgagga cgccgactgg
      661 ctctcgggcg atcaccggat ggagggggtg atcgctgcgg ccggcccgca cgtccgggcg
      721 gacgcgttcg acgagccggc gatgctgatc gacgtcgccc ccacggtgct cgccgcgctt
      781 ggcgtgccgc cgtcggtcaa gcatgacgga caggtgctga ccgccgtggt cggcgcggag
      841 gcgcaggcgg agacgaccgt ggccccaccc ccccgtccgc agggttccga cgtcgaggat
      901 ccctctggcc tcgacgagga agaggcccgg caggtcgagg atcacctccg cggccttggc
      961 tacctcgagt agcgagcccg ggccgcgtcc ccgcggcatc cgcgccctga tcgaagccgt
     1021 cctcccggct gcccgcttcg gtgacgagcg ggcgatcgcc gcgggcaccg ggcagaacct
     1081 cgtcggtctc gtcgtggcga tgctcgcgac cttcgcgacg caggtcctga tcacgcgcgt
     1141 cttgggggca gcggccttcg gggcggtcac ggtggcgacg cagctcgcgt tcgtcggcgc
     1201 ggcggccacg cggttcggga tggacatggc atccgtgcgc ctcgtcgcga tccacgcggg
     1261 gcgaggggag cgcgaggtgc tgcggggggt cgtgcgccga gcggcgggga tcgccgcgct
     1321 cgtcagcgtc gtcgtgggag ccggcatctt cgccggcgcc gacgcgctcg cgcggacgtt
     1381 cgcatccccc ggggaggggg gtgcgttccg tgcggcggcc atcgccctcc cgttcgcggc
     1441 gctcgtccac gtgtacctcg gagcgacgcg gggtctcaag gtcatgcggc acacgctcac
     1501 ggtctactgg gtgggccagc cgctgctgtg gatccttctg ctgctctcgg gatggctggt
     1561 cgcccggagc atcgagacca gcgtggccgc ctacgccggg tcgtgggcga tcgccacggt
     1621 ggccgcgctc ctgctgtggc gccgtgagac tcccggcccg gaggcaccgg tgccggcggg
     1681 cgagatgagg cagctggtcc gctacggagc cccgagggca cccgcggcgc tgtccgccca
     1741 gctgctgttc tggaccgacc tgttcgtcct cgcgcgcgtc gccccggcgg cggaggtggg
     1801 cgtgtacgcg gccgccgtgc gggccggaca ggtgatcctg ctgttcctca tctcggtcaa
     1861 caacatgttc agcccgttcg tcgccgacct ccacgcccga ggggagcgcc agcggctcga
     1921 cgccctgtac aagctggtca cgcgctggat gctggtggcg accctgccga tcctcatcgt
     1981 gctcgccgtg gtcccggacg ccgtcctgca cctgttcggg gaggggttcg ggcgcggacg
     2041 gacggcgctg ctcatcctgc tgggcgggca gctcgtgaac gtcgccacgg ggagcgtcgg
     2101 cttcatcctc atcatggtcg gccgcaccgg ctgggacctc gtcgtgaacg gcgtggcggt
     2161 cgcgttctcg ctgggggtcg cgatgctgct ggcgcctcgc tacgggatgg agggagcggc
     2221 cgtcgccggc gccacgacgc tcgcgctcgg gaacctgtgg cgactggccc tcgtccggcg
     2281 cttggtcggg atccagccct tcacgcgcga ccacctgcgc ctgctgccgc cggcgctcgc
     2341 gggggc
//
LOCUS       DATLGG010000308         6644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_123239, whole genome shotgun sequence.
ACCESSION   DATLGG010000308 DATLGG010000000
VERSION     DATLGG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123239"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1441
                     /locus_tag="VM638_08710"
     CDS             167..1441
                     /locus_tag="VM638_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12538.1"
                     /translation="MAPASSVRSVAADPAVRVGVGVTLVIMLGYGLIVPVLPLYARSF
                     GVGKAEVGLLLSSFALMRLVFDLVAGPLVDRAGPRAVATGGAVVVALSGALSALAPSF
                     GFLLAARAVGGAGSSLLFAAVMSHLLQAVPPGRMARTMSLYYASFLLGMVLGQPVGGL
                     IASIAGLAAPLWFYAGACLVSAGVTMRFLADRGPRGVAEAETPQEVLAGAEGGAPTAW
                     GRLRGLLRGRAFVAALVANAILFWVLGSVRLTLVPLFAGERLGLGAAQIGALLGVAAV
                     AQFLLMWKAGSLADARGRRLVLLPSLIVVAIGTALLGWTASALTLGAVLAVLGAATGF
                     SGVVPAAVVADVAPKRSSGTAVGAYRFAGDLGFVLGPFVAGGVAEAYGFRAAFVVIAV
                     PVLVAVALVGRMPETLSTQAANSSLGAGRPRK"
     gene            complement(1483..3375)
                     /locus_tag="VM638_08715"
                     /pseudo
     CDS             complement(1483..3375)
                     /locus_tag="VM638_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            3686..5488
                     /locus_tag="VM638_08720"
     CDS             3686..5488
                     /locus_tag="VM638_08720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12539.1"
                     /translation="MLLLGLLGLIIVSVPLAGGRLGRIADLRVRQPWVLAAALVAKFS
                     VILLFPGSPGPIHAAVHVGAYVAGGWFLWVNRDLPGMLLIALGGGMNFAAIAANGGVM
                     PASNAAFQAAGLAPVAGRFSNSAPMGDPNLGFLGDIFATPASWPLANVFSVGDIVLLL
                     GIGVALHRACGSRLVPSGRGQFSALFAHRDFIRVWGALGVSNIGDFVYSLAVYATLAQ
                     RGEAAHALAVLLIMQVAPAALTSALGGPLIDRAPRKALLIATDLARFLAVASLLIAPH
                     PSLVHMYVVAAALGVFGSLFQPTFLASLPNLVPKEQVVAANALVGATMHAAIMVGPVL
                     GGLLVSHLGPQIAFGVNAASFLVSAALIAPARVPQSVRVEVGAPMRDLVEGVRYSLGT
                     PLVRGALVVIGMVMAASYLRTPLEPLFVIRELGLRPEALGLVVGVWGLGMLLGSIAAP
                     TAARRWRRERLLAASIALIGLSVLTASRSGSLAVVLLLWLLSGAANGLGSVAYDSLLQ
                     ERTPDRLRGRVMGASEAVQQASILVGVFLSGWLGTNAGIRPTFFLSGVLLLVTAVVCR
                     TVLASAPGRHRRRSVQRARRLAAAGLAPARTSPL"
     gene            complement(5498..>6644)
                     /locus_tag="VM638_08725"
     CDS             complement(5498..>6644)
                     /locus_tag="VM638_08725"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM12540.1"
                     /translation="YSTNYLRREPIGVCAAITPWNAPIQMAAWKIAPAIAAGNTVVLK
                     PASYTSLTAIELGKICLEAGIPEGVVNVLPGPGGAVGEALVESPLVDKIAFTGSTEVG
                     SRIMQLAAGTIKKVTLELGGKSPSIVLDDADLDYAVDGALYGVFFHQGQICSAGTRLF
                     LQRGIHDEFLTALVKKASDMTVGPALDPASDQGPLISEGQLETVDGYVRKGIEEGAEV
                     ATGGERATVEGHEGGYYYRPTILTGVDNSMTVAQEEIFGPVLAVIPFEDEAEAVRLAN
                     ESIYGLGGAVWSRTVPRAVKVAEQLRTGTVWINDYHMINPRYPFGGYKRSGIGREHGK
                     VGFDEYREVKHIHVDMAGGREAHKWWDVVVPSASEGQTPKGETRPGG"
BASE COUNT          784 a         2408 c         2373 g         1079 t
ORIGIN      
        1 gccgagggga gccgatgatc cgggacgacg ccggtgcgcg acagcagctc catgaccatg
       61 agggccccgg atacgccgag ggctccatat gaccatcccg ggggacgcgg gcgcggcatc
      121 ctcggattat ccctctcacc ccggctaggg tgggctggtg cctgggatgg ctcccgcctc
      181 gtccgtgaga tccgtcgccg ccgatcccgc cgtgcgcgtc ggggtcggcg tcacgctcgt
      241 gatcatgctc gggtacgggc tgatcgttcc ggtcctcccg ctgtacgcgc ggtcgttcgg
      301 ggtgggcaag gccgaggtcg ggctgctgct ctcctcgttc gcgctgatgc ggctcgtctt
      361 cgacctcgtc gcggggcccc tcgtcgaccg cgcggggccg cgtgccgtcg ccaccggcgg
      421 cgcggtcgtg gtcgccctct ccggcgcgtt gtcggcgctc gccccctcgt tcgggttcct
      481 cctcgcggct cgggccgtcg ggggagcggg ctcctcgctc ctgttcgcgg ccgtgatgag
      541 ccatctgctc caggccgtgc ccccggggcg gatggcgcgc acgatgagcc tctactacgc
      601 gtcgttcctg ctcgggatgg tcctcggcca gccggtcggc ggcctgatcg cgtccatcgc
      661 cggcctggcc gcgccgctgt ggttctacgc cggggcctgc ctcgtctcgg ccggcgtgac
      721 gatgcggttc ctcgccgacc gcggcccccg cggcgtcgcc gaggccgaga cgccgcagga
      781 ggtcctcgcg ggagccgagg gcggggctcc gacggcgtgg gggcgcctcc gcgggttgct
      841 ccgcggacgg gccttcgtcg ccgcgctcgt ggcgaacgcg atcctgttct gggtgctcgg
      901 ctcggtccgg ctcaccctcg tccccctgtt cgccggtgag cggctggggc tgggggccgc
      961 gcagatcgga gcgctcctgg gcgtcgcggc cgtcgcgcag ttcctgctga tgtggaaagc
     1021 cggctcgctc gcggacgcgc gcggccgccg tctggtgctg ctcccctcgc tcatcgtcgt
     1081 cgcgatcggc acggcgctgc tcgggtggac cgcgagcgcc ctgacgctcg gtgcggtcct
     1141 cgcggtcctc ggagcggcga cggggttctc gggcgtcgtc ccggccgcgg tcgtcgcgga
     1201 cgtcgccccg aagcgatcct ccggcacggc ggtcggggcc taccggttcg ccggcgacct
     1261 cggcttcgtg ctcggtccgt tcgtcgccgg cggggtcgcc gaggcctacg ggttccgtgc
     1321 ggccttcgtg gtgatcgccg ttcccgtcct ggtggcggtc gcgctcgtcg ggcggatgcc
     1381 cgagacgctc tcgacccagg cggcgaactc gtcgctgggc gccggccgac ccaggaagta
     1441 gccctgcgcc atgtcgcagc cgaaggcttg gaggcgcccc cagacctcct cgctctccac
     1501 accctcggcc acgaccgcga gcccgaggtt gcgcgcgagg tcgatcgtcg actggacgat
     1561 cacggcgtcg tcgtcgtcga tcgtcatgtt gatcacgaac gacttgtcga tcttgatctc
     1621 gcgcaccggc aggcgcttga ggtaggcgag cgaggagtag ccggtcccga agtcgtcgag
     1681 ggcgagtccg acgcccatcg agctcagccg tccgagcacg cccatcgccc gcagcgggtc
     1741 cacgacgatc gcgctctcgg tgatctcgag gcgaagaacg ctgggatcga ggccgaacgt
     1801 gtcgaggagc ctcccgacct cctcggggaa ctccgagtcc tggaggttgc ggacggagag
     1861 gttgacgctg accccgaggt ccagacccct gctgccccag gcccggcact gttcgagcgc
     1921 gtcgtgcagg acgcgccggg tgagcggctt gatcagaccc gtgtgctccg cgagcgggat
     1981 gaactcgtcc ggcatcatca ggccgttcgt cgggtgcatc caccgcacga gcgcctccgc
     2041 cccgatcacg cggccgctcc gcatgtccgc catcggctgg tagtggagga tgaactgccc
     2101 gtcctcgatc gcacgccgca gctcacccag gagcgcgagc cgctgcgggc tgtggcggtc
     2161 ccgctccgcc gtatagagct cgatgccggt gtgcgcttcc ttcgccgtgt acatcgccac
     2221 gtcggcgcgc tgcatgagca tgtgcgggtc ggcgccgtgg tccgggcaga gcgcgatgcc
     2281 gatgctggcc tcgatgtcga gggcgagccc gtcgatcggg aacgggtcct cgagggcccg
     2341 cagcaccttg tccgcgaccg cgacggcccc ggcggcgtcg ctcacctccg gcagcagcac
     2401 cgcgaactcg tcgactccca tccgcgcgac ggtgtcgctg cgccggacga gcgcctggag
     2461 gcgggggccg atcatctgga ggagacggtc gccgaccggg tggccgagcg tgtcgttgat
     2521 ctccttgaac cggtcgaggt cgatcagcat caccgcggcc accgtccggt cgcggcgtga
     2581 tttcaggatc gcgtgcccga tccggtcctg gaacaggcga cggttcggca ggctcgtcag
     2641 cgggtcgtgg agcgcctgat gctccttgta ctgcttctcg agccagatcg ctgtggcgcg
     2701 gtaggccccg agcatcggga gcgcgagcag cggcacgagg gcgacgctgc gctcggcggc
     2761 gaccacgacg atgggcgaca gcgccagcat cacgagggtc gtcacggcct ggaacgagct
     2821 gtcctcccgc agagcgcgcc agaccggctg ttcctgcgcg agcgcgatcg ccgttcgcgt
     2881 cagcgcgtgg ttcacgacga agaagacggc ggccccgacg aggatcgccg gcaggtcgga
     2941 gatgaagaag gccggtgggg cgttggggtg cggcacgtgg gcgaccaggc gcaccgtgta
     3001 gccggcggcg accaccgcga tcgcgtactg cgccgcgttg aacagcgcct tccaccaggg
     3061 cttgcgccgg aagagctcac acgtgagggt cgcgacgacg tgtgcgatgg ccgtcgcggc
     3121 gggcccgaac gcgaggatca tcgcgtacgc gaaggtcccg gaggtcgtga tctcctcgtc
     3181 ctcgtcgcgg cgcgggaccc gcaccggaag gagctcaccg aggaacagga agaacgcgaa
     3241 gagccagaac tcgccgggcg cctcggcgac cggcgcgatc ccacggctga acgcgagcgc
     3301 cacgacgagc ccgacgcccg cgaccgagac cacggcgacg taccagtgga tcggacggat
     3361 cttcccgacc ttcatccgta cctctctctg gaatgggacg ccccggcgtc gccgccgggg
     3421 cgtcccaccg cgttcgggag gcctagcccc acctggcggc gccgaggacc gccaccagca
     3481 tcccgagcat cgacacggca agggtcagcc tgaagagccg agccttcatc cggccacctc
     3541 ctctcgtaca gagcggcccc cctcggggga cgcctcgatg cgggtatcgg gccaggcggc
     3601 ctagtccttt agcactacgt catcgcctca agcggccgtc gggcggtgcc gatacggggg
     3661 acgacccgct cgtcggagga gggccatgct gctgctcggc ctgctgggcc tcatcatcgt
     3721 ctcggtgccg ctcgcgggcg gccgcctcgg acgcatcgcg gacctgcgcg tgcgacagcc
     3781 gtgggtcctc gccgccgccc tcgtggcgaa gttctcggtg atcctcctct tccccgggtc
     3841 gcccggcccg atccacgcgg ccgtccacgt cggcgcctac gtcgccgggg ggtggttcct
     3901 ctgggtgaac cgggacctcc ccggcatgct gctgatcgcc ctgggcgggg gcatgaactt
     3961 cgccgccatc gccgcgaacg gaggagtgat gccggcctcg aacgcggcgt tccaggcggc
     4021 ggggctcgcg ccggtggcgg gccgcttctc gaactcggcg ccgatgggcg atccgaacct
     4081 cgggttcctc ggcgacatct tcgcgacgcc ggcgagctgg ccgctcgcga acgttttcag
     4141 cgtcggcgac atcgtcctgc tgctcgggat cggggtcgcg ctgcaccggg cgtgcgggtc
     4201 gcgtctggtc ccatccgggc gcggacagtt ctccgcgctc ttcgcgcacc gcgacttcat
     4261 ccgggtgtgg ggagcgctcg gcgtctcgaa catcggggac ttcgtctact cgctcgccgt
     4321 gtacgcgacc ctcgcgcagc ggggggaggc ggcccacgcg ctcgcggtcc tgctgatcat
     4381 gcaggtggcg ccggcggcgc tgaccagcgc gctcggcggg ccgctgatcg accgggcgcc
     4441 gcggaaggcg ctcttgatcg cgaccgacct cgcgcggttc ctcgccgtgg cctccctcct
     4501 catcgcgccc cacccgagcc tggtgcacat gtacgtcgtc gccgccgcgc tcggcgtctt
     4561 cgggagcctc ttccagccga cgttcctcgc gtcgctcccg aacctcgtcc cgaaggagca
     4621 ggtcgtcgcg gcgaacgccc tcgtcggggc gacgatgcac gccgcgatca tggtcggccc
     4681 ggtgctcggg ggcctgctcg tctcccacct ggggccgcag atcgcgttcg gcgtgaacgc
     4741 cgcgtcgttc ctcgtgagcg ctgcgctgat cgcgcccgcg cgcgtgcccc agtcggttcg
     4801 cgtcgaggtc ggggcgccga tgcgcgacct cgtcgaaggc gtccgctact cgctggggac
     4861 gccgctcgtc cgcggcgccc tcgtcgtgat cgggatggtg atggccgcgt cgtaccttcg
     4921 gaccccgctc gagccgctgt tcgtgatccg cgagctcggg ctccggccgg aggcgctcgg
     4981 gctcgtcgtg ggcgtctggg gcctcgggat gctgctcggc tcgatcgccg cgccgacggc
     5041 cgcgcgccgg tggcgtcgcg agcggctgct ggcagcgtcg atcgcgttga tcggcctgag
     5101 cgtgctcacc gcttcgcgtt ccggaagtct cgcggtcgtg cttctcctgt ggctgctgtc
     5161 gggcgccgcg aacgggctcg ggtccgtcgc ctacgactcg ctgctgcagg agcggacccc
     5221 tgaccgactg cgcggacgcg tgatgggcgc ctccgaggcg gtccagcagg cctcgatcct
     5281 cgtcggcgtc ttcctctctg gctggctcgg gacgaacgcc gggatccgcc cgaccttctt
     5341 cctgtcgggg gtcctgctgc tcgtgaccgc cgtcgtctgc cggacggtcc tcgcttcggc
     5401 cccgggccgg caccgccgtc ggtcggtgca gcgagcacgc cgcctcgccg ccgcgggact
     5461 cgctcccgcc cgaacctccc cgctgtagcg atccgggtca tccgccgggg cgcgtctcgc
     5521 ccttcggcgt ctggccctcg gaggcgctcg gcacgacgac gtcccaccac ttgtgggcct
     5581 cgcggccgcc ggccatgtcg acgtggatgt gcttcacctc gcggtactcg tcgaagccga
     5641 ccttgccgtg ctcgcggccg atcccggacc gcttgtagcc cccgaagggg tagcgcgggt
     5701 tgatcatgtg gtagtcgttg atccacaccg tgccggtccg aagctgctcg gcgaccttga
     5761 ccgcgcgcgg cacggtccgt gaccagaccg cgcccccgag cccgtagatg gactcgttcg
     5821 ccagccgcac cgcctcggcc tcgtcctcga acgggatgac cgcgagcacc ggaccgaaga
     5881 tctcctcctg ggcgacggtc atcgagttgt cgacgccggt gaggatcgtc ggccggtagt
     5941 agtagccgcc ctcgtggccc tcgaccgtcg cgcgctcgcc gccggtcgcc acctcggctc
     6001 cttcctcgat gcccttccgc acgtagccgt cgaccgtctc gagctgcccc tcggagatca
     6061 gcggcccctg gtccgaggcc gggtcgagcg ccgggcccac ggtcatgtcc gacgccttct
     6121 tcacgagcgc ggtgaggaac tcgtcgtgga tcccccgctg caggaacagc cgcgttcccg
     6181 ccgagcagat ctgcccctgg tggaagaaga ccccgtagag cgcgccgtcg accgcgtagt
     6241 cgaggtcggc gtcgtcgagc acgatcgatg gggacttccc gccgagctcg agcgtgacct
     6301 tcttgatggt ccccgcggcg agctgcatga tccgcgaccc gacctcggtc gaccccgtga
     6361 acgcgatctt gtcgacgagc ggcgactcga cgagcgcctc gccgacggcc ccgccgggcc
     6421 cggggagcac gttcacgacg ccctcgggga tccccgcctc gaggcagatc ttcccgagct
     6481 cgatcgccgt gagcgacgtg taggaagcgg gcttgaggac gacggtgttg ccggccgcga
     6541 tcgccggggc gatcttccat gccgccatct ggatcggggc gttccacggc gtgatcgcgg
     6601 cgcagacgcc gatcggctcg cgccggaggt agttcgtcga gtag
//
LOCUS       DATLGG010000309         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_106858, whole genome shotgun sequence.
ACCESSION   DATLGG010000309 DATLGG010000000
VERSION     DATLGG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106858"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="VM638_08730"
     CDS             complement(<1..1965)
                     /locus_tag="VM638_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12541.1"
                     /translation="MTRRPRGRLVSMLVTCLSAAGLLAPPPAGAAWQRGGVTERVSVA
                     SDGARGDDGSWFPFLSDGGRYVLFTSRAGNLAEGDTNGEADAFIRDRVEGLTERITGP
                     DGKQLSSAYGSSITPDGRYVVFGSPDRVLPGDTNNAEDVYLLDRASEAIHRISISSGG
                     IGGNDGSYVGVATPDARFVVFMSWASNLVTGDTNGQLDVFLHDRALRQTELISRSTTG
                     RQSDSRSLTTSQPVSADGRYVVFSSAATAFSPELPVASIQCYLRDRQAGTTEIVSLSN
                     DGVPGDGGCHEPVISANGRYVAFTSAATNLVPSDTNAALSRLCVYVRDLVAGKTYRVS
                     VSSAGEESNGWSSRPSISADGRYVAFDSSATNLWPGQTDVTNQVWVHDMHARTTEVVT
                     IPVPDGDSVNGHPPTYADGDGAALSPDGRMVGFSSTSDALLPDEQGRLSDVFVRDRGG
                     PDAIGGLRATVVGGDVRASGWVGLDARVLSASDDPIDADGGEVADRLGAEIQHARVDL
                     QPQEEAIALRMSLAHLAPVAARPPVSTTAHCNDTGICGARAGGGPAVVYGFGFEVEGR
                     RYEVRAMRAPATAGHSPASPNPYNSDSLKKPINPYIALYRCVVDCDRIAKLRGGIGDA
                     GVSVDTAVPLSDLGITGPTTIRDVRAFTAVG"
     gene            complement(1962..>3346)
                     /locus_tag="VM638_08735"
     CDS             complement(1962..>3346)
                     /locus_tag="VM638_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12542.1"
                     /translation="DTNGGPDVFEVHLSSGTIRKASVAGDGRQSDGDVGHPTISSDGR
                     YVGFHSSSHFVPAEPGGWSMYVQDRDQDRGRSFLARVSVSEDGSDGRSDPGPSGISDD
                     GRYAVFTSSAPGLVPDDGNGLADVFVHDRLGGTTERLSTRAGGGDASAPSYAGAISAD
                     GRFVAFVSEASDVTPGDANDRADVFVTDRGPDLGVSALEISRSGRRLGVSGSASFAPV
                     MLQSAWDTHYDVRPYDTAVGGQILWGDLAYRPESDELLARIRLASLPWLRSKVDCPAA
                     CPGGLPVMIYGAVFLIGGRTFEARAIRSVSTESKAQDRFELLRCSGWCQPVGTFRGSI
                     GSSGYEVNLLIPTASLGLGPGRKIDRVQLFARLAPSLQTGPYLPAMLLVDDVMFYETP
                     IPGTSVELGIAPAGADVVEYTVPAIVEAGRFEGSFDVARTPPGRYDVWTKVCAATCAE
                     TRTPHELEGA"
BASE COUNT          508 a         1192 c         1175 g          471 t
ORIGIN      
        1 tccgacggcc gtgaacgcgc gaacgtcccg gatcgtcgtg gggcccgtga tccccagatc
       61 cgacagggga accgccgtgt ccaccgagac gccggcatcg ccgatgccac cgcggagctt
      121 cgcgattcgg tcgcagtcca cgacgcagcg gtagagggcg atgtaggggt tgatcggctt
      181 cttgagcgaa tcgctgttgt aggggttcgg cgaggcggga ctgtggcccg ccgtggccgg
      241 agcccgcatc gcgcggacct cgtagcgtcg tccctcgacc tcgaacccga acccgtacac
      301 gacagctggt ccaccccccg ctcgcgcccc gcagatgccc gtgtcgttgc agtgcgcggt
      361 cgtggagacg ggtggcctcg cggcgacggg agcgagatgt gcgagcgaca tccgcagcgc
      421 gatcgcttct tcctgcggct ggaggtcgac gcgggcgtgc tggatctccg caccgagacg
      481 gtccgcgacc tcgccgccgt ccgcgtcgat gggatcgtcc gacgcggaga gcacccgcgc
      541 gtccagaccg acccagccgg acgcccgaac atcgcccccc acaaccgtcg cccgcaggcc
      601 gccgatggcg tccggtccac cccggtcacg gacgaagaca tcgctcagcc tgccttgctc
      661 gtcgggcagc aaggcatcgc tggtgctgga gaaaccgacc atgcggccgt ccggtgatag
      721 cgctgccccg tcgccgtcgg cgtacgtcgg cgggtgaccg ttcacgctgt cgccgtccgg
      781 gactgggatc gtcacgacct ccgtggtccg cgcgtgcatg tcgtggaccc agacctggtt
      841 ggtgacatcg gtctgaccgg gccagaggtt cgtggcgctg gagtcgaagg cgacgtagcg
      901 gccatccgcg ctgatcgagg ggcgagagga ccacccgttc gactcttcgc ccgcggaaga
      961 gaccgagacc cggtaggtct tgccggccac gaggtcccgc acgtagacgc agaggcggct
     1021 gagcgcggcg ttcgtgtcgc tcggaacaag gttggtcgcc gccgaggtga aggcgacgta
     1081 ccgcccgttc gcgctgatca ccggctcgtg gcagccgccg tcgcccggaa cgccgtcgtt
     1141 cgagagggag acgatctcgg tcgtccccgc ctgacggtcg cgcaggtagc attggatgct
     1201 ggcgaccggg agctcgggac tgaaggccgt ggcggcgctc gagaacacga cgtagcggcc
     1261 gtccgcgctc accggctgcg aggtcgtgag cgaccgagag tcggactgcc ggcctgtcgt
     1321 cgacctcgag atcagctccg tctgccgaag cgcgcggtcg tggaggaaga cgtcgagctg
     1381 accgttcgtg tcaccggtca cgaggttcga cgcccagctc atgaagacga cgaagcgggc
     1441 atccggcgtc gcgacgccga cgtaggaccc gtcgttcccg ccgatcccgc cggagctgat
     1501 cgagatgcgg tggatcgcct cggacgcgcg gtcgagcagg tacacgtcct ccgcattgtt
     1561 cgtgtcgccg gggagcaccc ggtccggcga cccgaacacg acgtaccggc catccggcgt
     1621 gatcgaagag ccgtacgcgg aggagagctg cttgccgtcc ggccccgtga tgcgctccgt
     1681 cagtccttcg acacggtcac ggatgaacgc atccgcctcc ccgttggtgt ccccctcggc
     1741 gaggttgccg gccctcgagg tgaagagcac gtaccgcccg ccgtcgctga ggaaggggaa
     1801 ccaggagccg tcgtcccctc gtgccccgtc ggaggcgacc gagacccgtt cggtcacgcc
     1861 gccgcgctgc caggccgcgc ccgccggcgg cggtgcgagc aggccggccg cgctcaggca
     1921 ggtgaccagc atggagacga gccgtcccct ggggcggcgc gtcacgcgcc ctccagctcg
     1981 tgcggcgttc gggtctccgc acacgtggcg gcgcacacct tcgtccacac gtcgtacctc
     2041 ccgggaggcg ttcgcgcgac gtcgaaggat ccctcgaacc gcccggcctc gacgatcgcc
     2101 ggaacggtgt actccaccac gtccgcgccg gcgggcgcga tccccagctc gacgctcgtc
     2161 ccggggatcg gcgtctcgta gaacatcacg tcgtccacca ggagcatcgc gggcaggtac
     2221 ggcccggtct ggagcgatgg cgccaggcgc gcgaacagct gcacgcggtc gatcttccgc
     2281 cccggtccga gcccgagcga ggcggtcggg atcagcaggt tcacctcgta gccgctcgat
     2341 ccgatgctcc ctcggaaggt cccgaccggc tggcaccagc ccgagcagcg gagcagctcg
     2401 aaccggtcct gcgccttcga ctcggtcgag acggaccgga tcgccctcgc ctcgaacgtg
     2461 cgacctccga tcaggaagac cgcgccgtag atcatcaccg gcagccctcc cgggcacgcg
     2521 gccggacagt ccaccttcga ccgcagccac ggcagcgacg ccagccggat gcgggcgagc
     2581 agctcatcgc tctcggggcg gtacgccagg tcaccccaca ggatctggcc tccgacggcg
     2641 gtgtcgtacg gccgcacgtc gtagtgggtg tcccaggccg actggagcat gacgggcgcg
     2701 aacgaggccg agcccgacac cccgagccgc cgtccggacc gagagatctc gagcgccgag
     2761 acgccgaggt ccgggccgcg atcggtcacg aagacgtccg cccgatcgtt cgcgtcaccg
     2821 ggcgtgacgt cggaggcttc gctgacgaac gccacgaacc ggccgtcggc ggagatcgcg
     2881 cccgcgtagg agggcgcgga cgcgtcgccg ccgcccgcgc gcgtcgagag ccgttcggtg
     2941 gtcccgccga gccgatcgtg gacgaacacg tccgccaggc cgttgccgtc gtccggcacg
     3001 aggcccgggg ccgagcttgt gaacacggcg tacctgccgt cgtcgctgat cccggagggc
     3061 ccagggtccg accggccatc cgatccatcc tccgagacgg agacgcgagc gaggaacgaa
     3121 cggccccggt cctggtcgcg atcttgcacg tacatcgacc agcctccggg ctccgcggga
     3181 acgaagtggg aggagctgtg gaacccgacg tagcggccat cggagctgat cgtcgggtgt
     3241 ccgacgtcac cgtcggactg gcgaccgtcg ccggccaccg aggccttgcg gatcgttccg
     3301 gaggagaggt gcacctcgaa cacgtcgggc ccgccgttcg tgtccc
//
LOCUS       DATLGG010000310         6746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_156285, whole genome shotgun sequence.
ACCESSION   DATLGG010000310 DATLGG010000000
VERSION     DATLGG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156285"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VM638_08740"
     CDS             complement(<1..548)
                     /locus_tag="VM638_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVM12543.1"
                     /translation="MRILILGGDGYLGWPTAMHFSNRGHAVHVVDNYLRREAHREQGT
                     DSLTPLAASLPERADAWREVTGYEIGVTEGDICDWDLVQRVFREFGPEAIIHYGEIPS
                     APYSMKSRDHAVFTQQNNVVNTLNVLWAMRDITPDAHLVKLGTMGEYGTPNIDIEEGF
                     IEIEHNGRRDTLPIPKLPGSLY"
     gene            complement(588..1793)
                     /locus_tag="VM638_08745"
     CDS             complement(588..1793)
                     /locus_tag="VM638_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVM12544.1"
                     /translation="MSVRLAWFTPLPPMPSGISDYSAELLPLVAEAAEVEIVCPPRRD
                     LRVPAGLRARTPETFDPGRVDAAIYHLGNNPQHVFAHDAALRHPGVCVLHDLTLHHLV
                     AYRFVEHERHPERYRAVLVEELGADLGRRVADLRLLGVATELEKFLFPLIGHVARRSR
                     GVVVHSRWGAERVAEVAPDVPTTVIPHHAGSPPPGLGGVDRAEARRRLGLPQDAFLVG
                     HFGFVNRPKQPSAVLGGMAVLRRSRPDARLLVVGADNTGGGLGRTARRFGIADAVRAT
                     GFVDLRAFYLHLKAVDAVVNLRYPSAGESSGTLARALAEGRAVVVNRLHAFAEIPDEA
                     AMKVEVDGDPAEQVGRHLVRLAEDPAERERVEAAARAYAARELDPIRCRDAYIRVAAD
                     AASVAGASK"
     gene            complement(1790..2140)
                     /locus_tag="VM638_08750"
     CDS             complement(1790..2140)
                     /locus_tag="VM638_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12545.1"
                     /translation="MALRDVAIRVLPASVVDRIRRRRAVRRYLHGMADELLERHARLE
                     SLEEQIATRPGGFYERVVKEVLDRTDVVLQQLDRRVEGLSGRQDDRLAALEREVAELR
                     RLLEDLRAGRAPGG"
     gene            2162..2482
                     /locus_tag="VM638_08755"
     CDS             2162..2482
                     /locus_tag="VM638_08755"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HVM12546.1"
                     /translation="MTAPEPEAYRVLPRTYVRFRRRSGTVTKRYDQTVEVEREAGMPA
                     AFSWRGRRYEVADVIGRWRIEGRWWADGRDREYWRVEARGGAVCDLYLDQRDGGWHLE
                     RLWD"
     gene            2492..5674
                     /locus_tag="VM638_08760"
     CDS             2492..5674
                     /locus_tag="VM638_08760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVM12547.1"
                     /translation="MGFVHLHCRSSFSLKDGAFLPEALARRAAELGMPAVAMTDRDSL
                     LGAARFVTACRDAGVRPILGAWVTVGHDRVLLLARDDDGYASLCRLLTHAHLSNERGD
                     PSVSAREVMERSAGLVCLLGPESPVGRLAIAGRPGAARERARPWREAFGPWCFVEVRD
                     LLEPESAREIRALLRLADDTGLPAVATNAVRYLVPGDAFLADALECMREIVPVAEHHV
                     SRANAEGWLKPPAAMRALFAERPDLCDATLRIAEACEVDLGLGRVHFPEFPVPAGRSA
                     TSVLAERAHAGIARRYGKATREVSERLDHELALIHRMGYAAYFLTVADIVEEVKAKGI
                     WCACRGSAAGSLVCYLTGISEVDPVEHGLLFERFLNPLRDELPDIDLDVESARREEVY
                     DLVLSRYGEDRAACVTMVDTFRARSAIREVGKALGYPDDEIDRAAKAFPHLGANRVRE
                     AMERLPELRDANLGHLERLFRVVERLDGFPRHLALHPSGIVLSGHDLVDRVPMERSFL
                     GHRMVQADKDDVEVLGLLKLDVLGVRMLSAMRHAVDEIERVEGERVDLDAIPVDDRPT
                     FELIRASDTIGCFQIESPGQRELLQKFQPTRFEDLIVDISLFRPGPVKSDMVTPFIRR
                     RHGWEAPTYAHPSLRPALGETHGVIVYHEQVMRVVDALTGCGLVEADRIRRHLDDGAL
                     IEAIHDEVLDRAVARGFDREQAQRIWDATLQFASFGFCKAHAAAFAVPTYRSAWLKAH
                     HPAAFLAGVLTHEPGMYPRRLILEDARERGIPILPLDVNASAAAYTVEPVGGGSLGIR
                     IGLQDVHGISEAEIAAILAGRPFDSVGDLLRRAAVSRPVVERLAHAGAFDRLPGGTRR
                     DRVFAAMTGETERPGEQLALALDGDPAPPGLRPYTAREVVRAELEVTGLDASRHLLSF
                     HEALLADLGVTRSVDLRRRRGDTHVMVAGVKVASQTPAVRSGQRIIFLTLDDAIGPVD
                     VTVFERVQERVARTVFHSFLLVVWGRLRRTGVAGASVVAEEVWDLQRLAVARREGRLR
                     EVMAEGAGPADDPRPGPRKLWHSSGGSAGW"
     gene            5671..>6746
                     /locus_tag="VM638_08765"
     CDS             5671..>6746
                     /locus_tag="VM638_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12548.1"
                     /translation="MSEDHEDVVPEIPLTRSPPSPARLLLRRPFITLVVAETLGNMGM
                     WAFFLGVEAKAAYGFGASASQLGLLLAAYSLVFLLAAPGLGILADRWSPKWMLVLAYT
                     ASIGCIAVALVGGSMPWMYLSLGLFAATHAAVWPARGALVPLLVDERYLVQANGMVGM
                     AWQVPLIAGPALAGLLIRLWGHGAPFVFAAAITATGVLTYLAVPDRRKEPEGAPERIL
                     PDLVAGFGEGFGTPELAWMLRRSVVGWVAMGLLITLEPLYVKEVLRRGQEVLGLVFSL
                     QGTGALVGSIVLARLREGRGREHPMITLGFIGGGLGFLLYVGTTSVLTACIGATLFGF
                     GFTFFTSPAQALVQRVARQPGKVTG"
BASE COUNT          832 a         2426 c         2511 g          977 t
ORIGIN      
        1 tggtagagcg accccgggag cttcgggatc ggcagcgtgt cccggcggcc gttgtgctcg
       61 atctcgatga agccctcctc gatgtcgatg ttcggcgtgc cgtactcgcc catcgtgccg
      121 agcttcacga ggtgcgcgtc gggggtgatg tcgcgcatcg cccacagcac gttcagcgtg
      181 ttgacgacgt tgttctgctg cgtgaacacg gcgtggtcgc ggctcttcat cgagtacggg
      241 gccgacggga tctcgccgta gtggatgatc gcctcgggcc cgaactcccg gaacacgcgc
      301 tggacgaggt cccagtcgca gatgtcaccc tcggtgacgc cgatctcgta gccggtcacc
      361 tcgcgccacg cgtcggcgcg ctccggcagg gatgcggcga gcggggtgag cgaatccgtt
      421 ccctgctcgc ggtgcgcctc ccgtcgcagg tagttgtcga cgacgtggac ggcgtgaccg
      481 cggttcgaga agtgcatcgc cgtcggccac ccgaggtagc cgtcgccgcc caggatcaag
      541 atgcgcatcg aagctccttg cgttccggtg atgcgggcac cccgatatta cttgctcgct
      601 ccggcgacgg acgccgcgtc cgccgccacc cggatgtacg catcgcggca ccggatcgga
      661 tcgagctccc tcgcggcgta ggcgcgggcg gccgcctcga cgcgctcccg ctcggccggg
      721 tcctccgcga ggcggaccag gtggcgaccg acctgctccg ccgggtcgcc gtcgacctcg
      781 accttcatcg cggcctcgtc ggggatctcg gcgaaggcgt ggagccggtt gacgacgacc
      841 gcgcgcccct cggccagcgc ccgcgcgagc gtcccggagg attcgccggc gctcgggtac
      901 cggaggttca cgaccgcgtc gaccgccttc aggtggaggt agaacgcccg gaggtcgacg
      961 aacccggtcg cccgcacggc gtccgcgatc ccgaagcggc gtgccgtccg gccgagcccg
     1021 ccgccggtgt tgtccgcgcc gaccacgagc agccgcgcgt ccggtcgcga gcgccggagc
     1081 acggccatcc cgccgagcac ggcgctcggc tgcttcggcc ggttcacgaa gccgaagtgc
     1141 ccgacgagga aggcgtcctg cggcaggccg agccggcggc gcgcctcggc gcgatccaca
     1201 ccccccagcc ccgggggtgg cgacccggcg tggtggggga tgaccgtcgt cggcacgtcc
     1261 ggcgccacct cggcgacccg ctccgctccc cagcgggaat gcacgaccac gccccgcgag
     1321 cgccgcgcga cgtggccgat cagcgggaag aggaacttct cgagctcggt cgcgaccccc
     1381 agcagccgga ggtcggcgac ccgccgcccg aggtcggctc cgagctcctc gacgagcacc
     1441 gcccggtacc gctccgggtg tcgctcgtgc tcgacgaagc ggtacgcgac gaggtgatgc
     1501 agcgtgaggt cgtggaggac gcagacgccc gggtgccgga gcgccgcgtc gtgggcgaag
     1561 acatgctgcg ggttgttccc gaggtggtag atcgcggcgt cgacgcgacc cggatcgaac
     1621 gtctccggtg tgcgtgcccg gagccccgcc ggcacgcgga ggtcgcggcg cggcgggcag
     1681 acgatctcga cctccgccgc ctcggcgacg agcgggagca gctcggcgga gtagtcggag
     1741 atgcccgacg gcatcggcgg caggggcgtg aaccaggcga gccgcacgct cacccgccgg
     1801 gcgcgcgccc ggcgcggaga tcctcgagga ggcggcgcag ctccgcgacc tcccgctcga
     1861 gcgcggcgag gcggtcgtcc tgtcgcccgc tcagaccctc gacgcgacgg tcgagctgct
     1921 gcaggacgac gtcggtccgg tcgagcacct ccttcacgac gcgctcgtag aagccgcccg
     1981 gccgcgtcgc gatctgctcc tcgagcgact cgagccgcgc gtggcgctcg aggagctcgt
     2041 cggccatccc gtggaggtag cgccggaccg cccggcgccg ccggatccgg tcgaccacgg
     2101 aggcggggag aacgcggatc gcgacgtcgc ggagggccat gacctgcgag tctagccggg
     2161 cttgacagca ccggagcccg aggcgtaccg tgtccttcct cgaacatacg ttcgattccg
     2221 acggaggagc gggacggtga cgaagcgcta cgaccagacg gtggaggtgg agcgggaggc
     2281 ggggatgccg gcggccttct cctggcgcgg aaggcggtac gaggtcgcgg acgtgatcgg
     2341 ccgatggcgg atcgagggac gctggtgggc cgacggccgc gaccgcgagt actggcgggt
     2401 cgaggcccgc ggcggcgccg tctgcgacct ctacctcgac cagcgcgacg gcggctggca
     2461 cctcgagcgc ctctgggact gaggcgcggc ggtgggcttc gtccacctgc actgccgctc
     2521 gtcgttctcg ctgaaggacg gggcgttcct cccggaggcg ctcgcccggc gagcggcgga
     2581 gctcgggatg cccgccgtgg cgatgaccga ccgggactcg ctcctcgggg cggcgcgctt
     2641 cgtcaccgcc tgccgcgatg ccggggtgcg gccgatcctc ggggcatggg tcacggtcgg
     2701 ccacgaccgc gtgctgctgc tcgcgcgcga cgacgacggc tacgcgagcc tctgccgcct
     2761 gctgacgcac gcgcacctct cgaacgagcg cggcgacccg tcggtcagcg cccgcgaggt
     2821 gatggagcga tccgccgggc tcgtctgcct gctcgggccg gagtcgccgg tcgggcggct
     2881 cgccatcgcg gggcgacccg gtgcggcccg ggagcgggca cggccgtggc gggaggcctt
     2941 cggcccctgg tgcttcgtcg aggtgcggga cctgctcgag ccggagagcg cgcgcgagat
     3001 ccgcgcgctg ctgcggctcg ccgacgacac gggcctgccg gcggtggcga cgaacgcggt
     3061 gcgctacctc gtccccggcg acgcgttcct cgccgacgcg ctcgagtgca tgcgcgagat
     3121 cgtgccggtc gccgagcacc acgtctcgcg cgcgaacgcc gagggctggc tgaagccgcc
     3181 cgcggcgatg cgcgcgctgt tcgcggagcg ccccgacctc tgcgacgcga cgctccgcat
     3241 cgccgaggcg tgcgaggtcg acctcgggct cgggcgcgtc cacttccccg agttccccgt
     3301 gccggccgga cgcagcgcga cgagcgtgct cgcggagcga gcgcacgccg gcatcgcgcg
     3361 acgctacggg aaggcgacgc gcgaggtgag cgagcgcctc gaccacgagc tcgcgctgat
     3421 ccaccgcatg ggctacgccg cctacttcct cacggtcgcc gacatcgtcg aggaggtgaa
     3481 ggcgaagggg atctggtgcg cctgccgcgg ctcggcggcg ggatccctcg tctgctacct
     3541 cacggggatc tccgaggtcg accccgtgga gcacgggctg ctgttcgagc gcttcctcaa
     3601 cccgttgcgc gacgagcttc ccgacatcga cctcgacgtc gagtccgctc ggcgcgagga
     3661 ggtctacgac ctcgtgctgt cgcgctacgg cgaggatcgc gcggcgtgcg tcacgatggt
     3721 cgacacgttc cgcgcgcggt cggcgatccg cgaggtgggg aaggcgctcg gctaccccga
     3781 cgacgagatc gaccgcgcgg cgaaggcctt cccccatctc ggcgcgaacc gcgtccgcga
     3841 ggcgatggag cgcctgcccg agctccgcga cgcgaacctc gggcacctcg agcggctctt
     3901 ccgcgtcgtc gagcggctcg acgggttccc gcggcacctc gcgctccatc cgtcggggat
     3961 cgtgctctcg ggccacgacc tcgtcgaccg cgtcccgatg gagcgctcct tcctcggcca
     4021 ccgcatggtg caggccgaca aggacgacgt cgaggtgctc ggtctcctca agctcgacgt
     4081 cctcggggtg cggatgctct cggcgatgcg tcacgccgtc gacgagatcg agcgggtcga
     4141 gggggagcgg gtcgacctcg atgcgatccc ggtcgacgac cggccgacgt tcgagctgat
     4201 ccgcgcctcc gacacgatcg ggtgcttcca gatcgaatca ccgggccagc gcgagctgct
     4261 ccagaagttc cagccgacgc ggttcgagga cctgatcgtc gacatctcgc tgttccgacc
     4321 cggcccggtg aagtcggaca tggtgacgcc gttcatccgg cggcgccacg ggtgggaggc
     4381 gccgacgtac gcgcacccct cgctgcgtcc ggcgctcggc gagacgcacg gcgtgatcgt
     4441 ctaccacgag caggtgatgc gggtcgtcga cgcgctcacc gggtgcgggc tcgtcgaggc
     4501 cgatcggatc cgccgccacc tcgacgacgg ggcgttgatc gaggcgatcc acgacgaggt
     4561 gctcgaccgc gccgtcgcgc gcgggttcga ccgcgagcag gcgcagcgca tctgggacgc
     4621 gacgctgcag ttcgcctcgt tcgggttctg caaggcgcac gccgcggcgt tcgcggttcc
     4681 gacgtaccgc tcggcgtggc tcaaggcgca ccatccggcg gcgttcctcg ccggagtcct
     4741 gacgcacgag cccgggatgt acccgcggcg gctgatcctc gaggatgcgc gcgagcgcgg
     4801 gatcccgatc ctgccgctgg acgtgaacgc gagcgcggcc gcctacaccg tcgaacccgt
     4861 cggcggggga tcgctcggga tccggatcgg gctccaggac gtgcacggga tctcggaggc
     4921 ggagatcgcc gcgatcctcg ctggccgccc gttcgactcg gtcggcgacc tcctgcggcg
     4981 tgcggccgtc tcgcgcccgg tcgtggagcg gctcgcgcac gccggcgcct tcgaccggct
     5041 gcccggcggg acgaggcgcg accgcgtctt cgccgcgatg accggcgaga ccgagcgacc
     5101 gggggagcag ctcgcgctcg ccctcgacgg tgacccggcg ccgcccgggc tgcgcccgta
     5161 cacggcccgc gaggtcgtcc gtgcggagct cgaggtcacg ggcctcgacg cgtcgcggca
     5221 cctgctctcg ttccacgagg ccctgctcgc cgatctcggc gtcacccgct ccgtcgacct
     5281 ccggcgccgt cgcggcgaca cgcacgtcat ggtcgcgggc gtgaaggtgg cgtcgcagac
     5341 gccggcggtc cgcagcggtc agcggatcat cttcctcacc ctcgacgacg cgatcggccc
     5401 cgtcgacgtg acggtcttcg agcgggtgca ggagcgggtc gcgcggaccg tcttccattc
     5461 gttcctgctc gtcgtctggg ggcggctgcg gcggaccggc gtcgccggcg cgagcgtcgt
     5521 cgccgaggag gtctgggacc tccagcggct cgcggtggcc cggcgagagg gtaggctccg
     5581 cgaggtcatg gccgagggcg cggggcccgc cgacgacccg cggcccggac cgcggaagct
     5641 ctggcactcg agcgggggat cggcgggatg gtgagcgagg accacgagga cgtcgtcccg
     5701 gagatcccgc tcacgcgttc gccgccctcg cccgcgcgcc tcctgctgcg ccggccgttc
     5761 atcacgctgg tcgtcgccga gaccctcggg aacatgggca tgtgggcctt cttcctcggc
     5821 gtcgaggcga aggccgccta cgggttcggc gcctcggcct cccagctcgg gctgctgctc
     5881 gcggcgtact cgctcgtctt cctgctcgcg gcgccgggcc tcgggatcct cgcggaccgc
     5941 tggagcccga agtggatgct cgtcctcgcc tacaccgcgt cgatcgggtg catcgcggtc
     6001 gcgctcgtcg gcggctcgat gccgtggatg tacctctccc tggggctctt cgcggcgacc
     6061 catgccgcgg tctggccggc acggggtgcg ctcgtgcccc tgctcgtcga cgagcgctac
     6121 ctcgtccagg cgaacgggat ggtcgggatg gcctggcagg tcccgctgat cgccgggccg
     6181 gcgctcgccg ggctgctgat ccggctctgg gggcacggcg cgccgttcgt cttcgcggcg
     6241 gcgatcacgg cgaccggggt gctgacctac ctggcggtgc ccgatcgccg gaaggagccg
     6301 gagggcgccc ccgagcggat cctgccggat ctcgtcgccg gcttcggcga ggggttcggg
     6361 acgccggagc tcgcgtggat gctccgtcgg tcggtcgtcg gatgggtcgc gatgggcctg
     6421 ctgatcaccc tcgagccgct ctacgtcaag gaggtgctgc ggcgcgggca ggaggtgctc
     6481 ggcctcgtct tctcgctcca ggggaccgga gcgctcgtcg gttcgatcgt gctcgcccgt
     6541 ctccgcgagg gccgggggcg cgagcatccg atgatcaccc tcggcttcat cgggggcggc
     6601 ctcggattcc tcctctacgt cgggacgacg tcggtcctca ccgcgtgcat cggcgcgacg
     6661 ctcttcggct tcgggttcac gttcttcacc tcgcccgcgc aggcgctcgt ccaacgggtc
     6721 gcgcggcagc ccgggaaggt gacggg
//
LOCUS       DATLGG010000311         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_156322, whole genome shotgun sequence.
ACCESSION   DATLGG010000311 DATLGG010000000
VERSION     DATLGG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156322"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="tyrS"
                     /locus_tag="VM638_08770"
     CDS             complement(<1..915)
                     /gene="tyrS"
                     /locus_tag="VM638_08770"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVM12549.1"
                     /translation="MFAPSDEQLERLLVGAADVIPEGDLRKRLDRASAEGRPLRVKLG
                     IDPSRPDLHLGHAVVLRKLRAFQDLGHVAVLIIGDFTGRVGDPSGRSETRPFLGEEEI
                     EANAATYLDQAGAVLHLEAAEVRRNSEWLAGLGMADLLELTATTTVARLLERDDFQGR
                     YREGRPISLVEFLYPVMQAYDSVAVRADVEMGGTDQTFNLLVGRDVQERYGQRPQVVF
                     TMPLLEGTDGVRKMSKSFDNFVGLTEPPEEQFGKLMSIPDELIGRYLRLCTDLPEAEV
                     EAVERGLGDGSAHPAEEKRRLAERVVALY"
     gene            968..>2208
                     /locus_tag="VM638_08775"
     CDS             968..>2208
                     /locus_tag="VM638_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVM12550.1"
                     /translation="MSPPSPFGRAARFAVLVLSLASVATACRMPNLDIPLEARPLPQN
                     SVIYAADGTRITTLHAEEDRVILPLERIPKVVQDAVIAVEDQRFWTHRGVDLRAMLRA
                     AVVNASEGRIVEGGSTITQQYVKNAILGSERTLDRKVREATVAWRLERTLSKAEILER
                     YLNTIYFGNGAYGVQSAARRYFSKRGADLRLREAALLAGLIAGPERYDPFEAPAAALQ
                     RRNLVLDRMLAQGMIAPQAHAEAVASPLGLEPPAPRERYPAAHFVEFVKKRILSDERF
                     GATREDRYNALFKGGLRIHTTVDPRLQRAADEAVNGILSQKGDPHGALTAIDPRTGRI
                     VAMVGGRDFFAKRKRNPVARVNLATGDGGTGRQAGSSFKPFALVAALENGMSPKKRYP
                     GGSSIAIPMAHGSPWLVRNYEG"
BASE COUNT          326 a          796 c          774 g          312 t
ORIGIN      
        1 gtacagcgcg acgacccgct ccgcgaggcg acgcttctcc tccgccggat gggccgaccc
       61 gtcgccgagc ccccgctcca ccgcctcgac ctccgcctcc ggcagatcgg tgcagaggcg
      121 gaggtagcgg ccgatcagct cgtcggggat gctcatcagc ttgccgaact gctcctcggg
      181 gggctcggtc aggccgacga agttgtcgaa ggacttcgac atcttgcgga cgccgtcggt
      241 cccctcgagc agcggcatcg tgaacaccac ctgcggccgc tggccgtagc gctcctggac
      301 gtcgcgcccg accagcaggt tgaaggtctg gtcggtcccg cccatctcca catcggcccg
      361 gaccgcgacg gaatcgtagg cctgcatgac cgggtacagg aactcgacga ggctgatggg
      421 ccgcccctcc cggtagcggc cctggaagtc gtcgcgctcg agcagccgtg cgaccgtcgt
      481 ggtggcggtg agctcgagga ggtccgccat cccgagtccg gcgagccact ccgagttccg
      541 gcgcacctcg gccgcctcga ggtggaggac ggcgccggcc tggtcgaggt aggtcgcggc
      601 gttcgcctcg atctcctcct ccccgaggaa cggccgggtc tccgagcggc cgctcgggtc
      661 ccccacccgc ccggtgaagt cgccgatgat cagcaccgcg acgtgcccga gatcctggaa
      721 ggcccgcagc ttccgcagga cgacggcgtg gccgagatgc aggtcggggc gggatgggtc
      781 gatgccgagc ttcacgcgga ggggccggcc ttcggcggag gcgcggtcca gacgctttcg
      841 caggtcaccc tcggggatca cgtccgccgc ccccacgagc aggcgctcga gctgctcgtc
      901 ggacggcgcg aacatgtcgc ggatgctacc aagaggcgcc ggaacccgtg ggtatactcg
      961 agcggcgatg tcgcctcctt ccccgttcgg gcgagccgcc aggttcgccg tactcgtgct
     1021 ctcgctcgcc tcggtggcga ccgcctgtcg gatgccgaac ctcgacatcc cgctggaagc
     1081 gcgcccccta ccgcagaact cggtgatcta cgccgcggac ggcacccgga tcacgaccct
     1141 gcatgccgag gaggaccggg tgatcctgcc gctcgagcgc atcccgaagg tcgtccagga
     1201 cgccgtgatc gccgtcgagg accagcgctt ctggacccac cgcggggtgg acctgcgcgc
     1261 gatgctgcga gccgccgtcg tgaacgcatc ggagggcagg atcgtcgagg gagggtccac
     1321 gatcacgcag cagtacgtca agaacgcgat cctcggctcg gagcggaccc tcgaccgcaa
     1381 ggtccgtgaa gcgaccgtgg cgtggaggct cgagcggacg ctttccaagg ccgagatcct
     1441 cgagcgctac ctcaacacga tctacttcgg gaacggcgcc tacggcgtgc agtcggcggc
     1501 ccggcggtac ttctcgaagc ggggcgccga cctccgcctc cgcgaggcgg cgctgctcgc
     1561 gggcctgatc gcgggccccg agcggtacga ccccttcgag gcgcccgccg cggcgctcca
     1621 gcggcggaac ctcgtcctcg accgcatgct cgcgcagggg atgatcgctc cgcaggcgca
     1681 cgcggaggcc gtggcctcgc cgctcggcct cgagcctccc gctccgcgcg agcggtaccc
     1741 ggccgcgcac ttcgtggagt tcgtgaagaa gcggatcctc tccgacgagc gcttcggcgc
     1801 cacgagggag gaccgctaca acgcgttgtt caaggggggg ctgcggatcc acacgaccgt
     1861 cgatccgcgc ctgcagcgcg ccgcggacga ggcggtgaac ggcatcctca gccagaaggg
     1921 cgacccgcac ggggccctga cggcgatcga cccgcgcacg ggccggatcg tcgcgatggt
     1981 cggcgggcgg gacttcttcg cgaagcggaa gcggaacccg gtcgcccggg tcaacctcgc
     2041 gaccggtgac ggcggcaccg gccgtcaagc cggctcttcg ttcaagccgt tcgcgctcgt
     2101 cgcggcgctc gagaacggga tgtctccgaa gaagcgctac cccggggggt cctcgatcgc
     2161 catcccgatg gcgcatggct caccctggct cgtgcgcaac tacgaggg
//
LOCUS       DATLGG010000312         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_106912, whole genome shotgun sequence.
ACCESSION   DATLGG010000312 DATLGG010000000
VERSION     DATLGG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106912"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..285
                     /locus_tag="VM638_08780"
     tRNA            194..285
                     /locus_tag="VM638_08780"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:229..231,aa:Sec,seq:tca)
     gene            complement(649..>2125)
                     /locus_tag="VM638_08785"
     CDS             complement(649..>2125)
                     /locus_tag="VM638_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12551.1"
                     /translation="DENGNPMYEREVRDFAVVGGYGPATRIVDITNPRDPVLVKTGPC
                     SASQGDIQVGHGLLFIAQDGGTGRCQRPGMSDATFNGTAVLDFSNPREPVYLSALPYG
                     RGSHNQTVHPTKPFVYLSDSDLAHTGLGNIPVWDVSNPSSPKLATEFKFGPHSPHDIT
                     FNADGSRAYAAAVSLTYILNTENPAAPTLVSVIPNEGITISHQSDPTPDGNFLLVSDE
                     VGGGAAGASPGGPAYVYDIRNETRPVRVGAIWDDCVGSSITCDQAGGAGLVSTAHVFR
                     INPDGYTMAIGWYTDGIHVIDYSGLTAGYNAAGLGAVTNVGSRTIARMKLPGRNNWAA
                     KMWQERHPGYVFATDMASSGGLDIFHVPELGVTDKGTFLAAGKLTGPNPLVYEAGVGV
                     TKTDFEENCEYSPRLNGLDGWVTKVPAGLAGKEMTVTDANGSLDYDLDLYFYDANCNL
                     TGMSESAGTEETGQIPAGTRYALAGAWLNVPAAQSIYFYVK"
BASE COUNT          293 a          720 c          761 g          351 t
ORIGIN      
        1 acacggcgtc ggcgcgcgag acggcgccgg ggcggcggcg gcagggaacg gggcgccgca
       61 gagtgccacc agcagcgtgg ccgacgccag gaccaccgag cgcttgttca cctggagtgg
      121 attcggcgcg tgccccggag actcctgcga gctggagccc gccatcttcc gccgctgtct
      181 gtaccctcgt cgcggagggg tctgggcgcc tggtgggccc cccggtcttc aaaaccggtg
      241 agcgggcgga agcccgctgg cgggttcgat tcccgtcccc ctccgctccg acggccggct
      301 gctacttggc ctgtcgaacg acccgctcga ggctcgcgag cagacggtcg tagcgacgct
      361 cgagcgcggt cacgcgtttc gccaattcac gatcccccgt cccgcgcgtc gtgcctgtgc
      421 cgccaccctg gcgacgctgt cgctgcgaga cgacgccgtg agcggcgctg cgatgccgac
      481 cgagcccggc gggatgtgcg gccttgaacc cgcatgtggg acacacgagg ttgcggcctc
      541 gtgcggatgt ggacttccgt cgttgtgcca taccgccagc gtaccggacg gaagaggccc
      601 gggcgggatg tcccgcccgg gcctccgggt gttcgttggg cgtcgctgct acttcacgta
      661 gaagtagatc gactgtgccg ccgggacgtt cagccacgcg cccgccagcg cgtagcgcgt
      721 gcccgccggg atctggcccg tttcctccgt acccgcggac tccgacatgc cggtgaggtt
      781 gcagttggcg tcgtagaagt acaggtcgag gtcgtagtcg aggctgccgt tggcgtccgt
      841 gaccgtcatc tccttgcccg cgagcccggc agggaccttc gtcacccacc cgtcgaggcc
      901 gttgaggcgc ggggagtact cgcagttctc ctcgaagtcg gtcttggtaa cgcccacacc
      961 ggcctcgtag accagcgggt tcggaccggt cagcttcccg gccgcgagga aggtgccctt
     1021 gtcggtcaca ccgagctcgg gcacgtggaa gatgtcgagg ccgcccgagc tcgccatgtc
     1081 cgtggcgaac acgtatccgg ggtggcgctc ctgccacatc ttcgccgccc agttgttgcg
     1141 gcccggcagc ttcatccgcg cgatcgtgcg cgacccgacg ttcgtcaccg cgccgaggcc
     1201 ggccgcgttg tagcccgcgg tcagacccga gtagtcgatc acgtggatcc cgtcggtgta
     1261 ccagccgatc gccatcgtgt agccgtccgg gttgatccgg aacacgtggg ccgtggagac
     1321 caagcccgcg ccgccggcct ggtcgcaggt gatcgacgag ccgacgcagt cgtcccagat
     1381 cgcgccgact cgcaccggcc gcgtctcgtt gcggatgtcg tacacgtacg cgggaccgcc
     1441 cggggaggca ccggccgcgc cgccaccgac ctcatcgctg acgaggagga agttcccgtc
     1501 cggcgtgggg tcagactggt gcgagatcgt gatgccctcg ttcgggatca ccgacacgag
     1561 ggtcggcgcc gccgggttct ccgtgttgag gatgtaggtc agggagacgg cggccgcgta
     1621 cgcccggctc ccatcggcgt tgaacgtgat gtcgtgcggc gagtggggtc cgaacttgaa
     1681 ctccgtcgcg agcttcggcg aggaggggtt cgacacgtcc cacaccggga tgttgcccag
     1741 gccggtgtgc gctagatcgg agtcggagag gtacacgaac ggcttcgtcg ggtgcacggt
     1801 ctggttgtgc gaaccccgcc cgtaggggag cgcggagagg tagaccggct cgcgcgggtt
     1861 ggagaagtcg agcaccgcgg tgccgttgaa cgtcgcgtcg ctcatccccg gacgctggca
     1921 cctgccggtg ccgccgtcct gcgcgatgaa cagcaggccg tggccgacct ggatgtcgcc
     1981 ctggctcgcg gagcaggggc cggtcttgac cagcacgggg tcacgcgggt tcgtgatgtc
     2041 gacgatccgc gtcgcggggc cgtagccgcc gacgacggcg aagtcgcgca cctcacgctc
     2101 gtacatcggg ttgccgttct catcc
//
LOCUS       DATLGG010000313        14656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_123285, whole genome shotgun sequence.
ACCESSION   DATLGG010000313 DATLGG010000000
VERSION     DATLGG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123285"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VM638_08790"
     CDS             <1..201
                     /locus_tag="VM638_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12552.1"
                     /translation="EYDKAEAMLEAYRHATEIERKGAVMFGNEMIDEASRKMAEQLAA
                     RGAAAGLSPQKTWDDFKEEWQA"
     gene            198..1373
                     /locus_tag="VM638_08795"
     CDS             198..1373
                     /locus_tag="VM638_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVM12553.1"
                     /translation="MIELTEDQQAIVDAVREFVEQEVIPVADEMEHRDEFPDKIVEQL
                     KEMGLFGLTIPEEFGGSGMDTMTYALVVAELSRGWMSISGVLNTHFMAAYLLNKHGTD
                     EQKQRWLPKMATGELRAAYSMSEPGAGSDVQAITTRAERDGDDYVVNGTKMWVTNGLR
                     AGLVVTLVKTDPDADPPAKGMSLLYVEKEPNAERFEGITVPPNIQKMGYKGVETTELV
                     FENHRVPAANLLGEEEGKGFYQVMDGIEVGRVNVSGRAVGLATRAFEEAIRYAQTREA
                     FGKPIAKHQGIQFKLAQMGTKLEAMRLMLLSAAKKKASGERSDLEAGMAKLFCSEMCK
                     EVVEDALRIHGGYGYSAEYTVERLYREAPFLLIGEGTSEIQQVIIARQLLERFKIQA"
     gene            complement(1370..2311)
                     /locus_tag="VM638_08800"
     CDS             complement(1370..2311)
                     /locus_tag="VM638_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVM12554.1"
                     /translation="MPDVRRVLITGGCGFIGANLVRILAERDLEIRVVDDLRKGDVSR
                     IPEGAAEVTIGDCADPAVMDPALEGVDAVVHLASETGVAPSVEDPVTDFEGNLATTFR
                     VLEACRRAGVERLVFASTGAAAGDVEPPIHEEVVPRPLSPYGAGKLGGEAYCHAYASA
                     FGMRTAALRFSNVYGPHSARKTWTAVPSFIMKCLTGEDITIYGDGSQTRDFIYVDDLC
                     DAIVRAMDADELAGEVFQVATGVETPIRDVAELVRNVVGADVEIRFEGRRPGEAYRTS
                     VDISKIRRMLGFDPKVDLEEGLRRTADWYRENWRPAS"
     gene            2389..3441
                     /locus_tag="VM638_08805"
     CDS             2389..3441
                     /locus_tag="VM638_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVM12555.1"
                     /translation="MNILRIIGFLVALLLVVQAIRRYRRRSLRLPDTAIMLLLAAGLA
                     TLSIAPALIDPVLRVLGFPPGNARRVIGVLVVSGILTYILLFRSFAKTDRLEQTLGTY
                     ADRVAAREFIREYGHPQPPADRKKLAVVIPALNEETSLPGVMKDVATEVEGMEVEIVV
                     VSDGSVDKTEQVAEEHGALVVRRDLRRGQGAAVRLGYHMALQRGADIVATVDADGQYD
                     PLELPQLVRPILDGEADVAHGSRMLGEYAKPIFGRSQGVRVFAWLTTRMAGHRITDPA
                     SGFRAFSAEALRQLEFRENQFHASEVTVAAAKAGLRVKEVPCTFRERHSGNSKKPPLL
                     RYGWGYARSLIRTWLG"
     gene            complement(3445..4632)
                     /locus_tag="VM638_08810"
     CDS             complement(3445..4632)
                     /locus_tag="VM638_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017303624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVM12556.1"
                     /translation="MKILILGGDGYLGWPTAMYLSNKGHDVAVVDSFHRRLWDQEIGA
                     DSLIPIAPLHDRVRVWNELGRGRIEMYIGDITEWDFLEHVWKDFDPESIVHYAEHRAA
                     PFSMLSRRHAVETQVNNVVGTLNILYAMKDLTPEAHLVKLGTMGEYGTPNIDIEEGFI
                     EIEHNGRKDVVLYPKKPPSFYHLSKVHDSHNIEFVCRAWGLRSTDLNQGVVYGVHTDE
                     TALDPRLQTRFDYDDVWGTALNRFCVQAAIDHPITVYGKGGQTRGFLDIRDTLQCVEI
                     ACNNPPEMGTYEVFNQFTEQFSVGELAEKVADGARKLGWEPMIENVPNPRVELEEHYY
                     NAKHTKLLDLGLQPYLLSETLIDGLLATVKQHADRVSRRLLYPSHNWKDGLLKDVQPG
                     EKV"
     gene            4685..5188
                     /locus_tag="VM638_08815"
     CDS             4685..5188
                     /locus_tag="VM638_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVM12557.1"
                     /translation="MGTAREFGRFLEDFEVGDVYKHWPGKTITEADDHLFCLITMNHH
                     PLHLDANYAETQTQFKRNVVVGNLVYSLALGMSVPDVSGRAIANLEVESLQHKAPMFH
                     GDTLYAETTVVDKRGSSSKPDRGVVTVETRGYNQDGTEVVFFRRKVMIPKRGSTEEQP
                     GRPTPQA"
     gene            complement(5185..6294)
                     /locus_tag="VM638_08820"
     CDS             complement(5185..6294)
                     /locus_tag="VM638_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVM12558.1"
                     /translation="MVGNGAARIRVLVVDDAAESRAALRRALSFDERIDVVGEAGSGA
                     QALRQADDLVPDVILMDVQMPDGNGVEATSAIHLRHPATRVIALTAHEDAETVREMLA
                     AGAAGYVVKGTAIDDLAAAVRSAHAGEGPIDDRILPHVLDDLRRLLGEERQRRGEAER
                     LARMREEFVHVLAHELRTPLTVMSGALRTVEQVVPREDVAALVRSALRRGDQLEHLIE
                     GLELIGDPGDGNPQANPARAVRDASRKLEETPDRLDLRDEDWPTIPERHLTRVAYELT
                     ANALRHGRRPVSIEARREGGEGVLRVRDAGGTDVDPALFRPFVQEDMSTTRERGGMGL
                     GLFVASRLCEAAGGTLRILRIDGETVAEARFPLRR"
     gene            6396..7067
                     /locus_tag="VM638_08825"
     CDS             6396..7067
                     /locus_tag="VM638_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVM12559.1"
                     /translation="MSETLDGIAREVVTCTRCPRLVAWREEAARRPPKRYEGERYWAR
                     PLPGFGDPRARILVVGLAPAAHGGNRTGRIFTGDRSGDFLFASLHRTGYANQPTSTHR
                     DDGLRLRDVYIAAVNRCAPPGNRPTPEERDNCLPYLVRELRALPNVRCIVCLGMFAWN
                     GAIRALAALGHPLPRPRPVFGHVADVQVGPYRLVGCYHPSQQNTFTGVLTEQMLDDVF
                     ARACS"
     gene            7150..8973
                     /locus_tag="VM638_08830"
     CDS             7150..8973
                     /locus_tag="VM638_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12560.1"
                     /translation="MTKASLRRVARTFSPYRGRIALVGIAILVTSTLGVINPILIKIL
                     FDRALFCAEGCVNLDLLYGLVALMVAIPVVSGVIGIGQTYLSNLVGQRVMQDLRNGLY
                     AHLQRMSLRFFTATRTGEIQSRLSNDVAGVQSVVTDTASSLLANVVIVISTVAAMAIL
                     SWQLTLLSLGMLPFFFWLTYRVGKARREVAASTQKSFADLSAITEETLSVSGILLSKA
                     FGRQQHETERFRSENRRLAGLQIRQQMIGRSFFATIQIFFSVTPALVYLVAGIVIAGN
                     PAGAGLTAGTIVAFTTLQSRLFFPVGQMLQVGVEVQSAMALFDRIFEYLDMEHEIQDA
                     PDAVEVPKDTVEGRVRFRDVSFRYGEQWTLRDIDLEIEPGQLAALVGPSGAGKTTMTY
                     LVPRFYDVQEGAVEIDGKDVRGIKLESLGEIVGMVTQETYLFHSTVAENLRYGNPAAT
                     DAELETAARAAFIHDRIVELTDGYDTVVGERGYKLSGGEKQRLAIARVILKDPAILIL
                     DEATSSLDTTSERLVQEALRPLMAGRTTIAIAHRLSTILAADVIFVVDRGRIVERGTH
                     EDLLRRSGLYARLYQQQFHGGEVEAECEDGVVLASGEVVRR"
     gene            8973..9605
                     /gene="pgl"
                     /locus_tag="VM638_08835"
     CDS             8973..9605
                     /gene="pgl"
                     /locus_tag="VM638_08835"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HVM12561.1"
                     /translation="MPPELVETDDLTAAALALFVEADPRRIVLAGGETPRPVYERLRE
                     VDHPWAETHVLMGDERCVPLDHPASNFGMAREALLAHVPAVAHPMPVGSCDPGAYESV
                     VRDALGDPPRVDLAFLGIGEDGHTASLFPGDPALAETERLVVRVDRPDHPRLTLTLPV
                     LSSARLAIFLVDGPSKGVAMRRLLAGEEIPASRVRAERVLVLATPRVTRG"
     gene            complement(9592..10353)
                     /locus_tag="VM638_08840"
     CDS             complement(9592..10353)
                     /locus_tag="VM638_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HVM12562.1"
                     /translation="MRASTDRRAPEIVPHQGPVPAEGEIEHIIGGRSGALRAAIFGAN
                     DGLVSNLSLIMGVAGAGLAPEVIVLAGVAGLLAGAFSMGAGEYISMRVQRELFERLIH
                     LEAHELAMDPDGERDELAEILRARGIPPDLASETAAALMKDPRVALEVHAKEELGLDP
                     TELGSPWGAAISSFTAFSLGAFTPLLPFLVASGFAATVAAIVLSLAALFTLGAAMTRF
                     TGRPWLTSGLRMLAIGGGAALVTYWVGRLIGVTLG"
     gene            complement(10350..11207)
                     /locus_tag="VM638_08845"
     CDS             complement(10350..11207)
                     /locus_tag="VM638_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HVM12563.1"
                     /translation="MTDAVRFEWRPHLDGGTLLCAFHGWNDGGDAATTAAAYLRDRWG
                     ARRFAGIDPEPFYDFQVTRPTVRLEGGVTRRIEWPENDFFHASLPEREMAVLLGIEPN
                     VRWRSFSSTIVETAQALGARSLVTLGAFLADVPHSVPPPVAGTAADEETAARLGVVPS
                     RYEGPTGIVGVLQDAAARAGLDSVSLWAAVPHYLPAGTNPKAALALVQKLSVYLGTTV
                     ETDTLARAAATWEAQVATMIEENAELQAYVQRLEEGVGGTDFEVPSGEALAAELERFL
                     REQRNDGDA"
     gene            complement(11239..12558)
                     /locus_tag="VM638_08850"
     CDS             complement(11239..12558)
                     /locus_tag="VM638_08850"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HVM12564.1"
                     /translation="MGRALLTDLYELNMAASYLQRGMTGPATFSLFIRGLPRGWGFFV
                     ASGLGPALEWLRDLRFEEEDLAYLRGTHGYPDDVLDRFRDLRFTGEVRAVPEGRVVFP
                     NAPLLEVTAPLPEAQLAESFLLNQITYQTAVASKAARCVLAAPDREMVDFSFRRVHGV
                     DAGMAVARATAIAGFAATSNVEAARLLGLRATGTMAHSYIEAFVSEEAAFRAFAEDFP
                     DSVVLLVDTYDTARGVRTAIEVGRELEGRGLRLAAIRLDSGDLAVLARDAREALDAAG
                     LREVRIIASGGLDEHRVADLASAPIDAFGIGSKVGVASDAPYLDSVYKLVEYEGRPVV
                     KLSTGKATLPGRKQVWRTSGGDVVGLMHEPAPTGGEPLLVEVMRDGAIVGDHSLAAAR
                     DRFRRERDWLPQALRALDADPLAVRRTEALDRLTERVREEIRAGARG"
     gene            12595..13092
                     /locus_tag="VM638_08855"
     CDS             12595..13092
                     /locus_tag="VM638_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HVM12565.1"
                     /translation="MSVPGERRKPPPKRERPRDELGRPLPWGSENRLQMEDFDALPVA
                     ENHRLGIEHFNARRFFPAHEAWETAWKQSRGTPDEEFYKGLSQLGAGYVHFSRGNLHG
                     THTLIRRATSRLRRYAPVHGRIDLAALVEQVEEQAATVERSAASGEGMPPLDPPRIAA
                     GTIDP"
     gene            13089..14525
                     /gene="thiI"
                     /locus_tag="VM638_08860"
     CDS             13089..14525
                     /gene="thiI"
                     /locus_tag="VM638_08860"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HVM12566.1"
                     /translation="MTRRLMVKIAGEIGTKSPRTRRRFLRILAGNVRAALDVAGVEGD
                     VEQRWSRLFVTTDRPTEAREALAGVFGIHSISEVAVVRFRDLDDLVASLSDLYRDRVT
                     GRTFAVRARRSGEPSFRSGDLAVALGSALLPHSAGVNLDDPDVEVPVEVVRGMAYAVL
                     GVTPGAKGLPLGSGHRAAALFSGGFDSPVAAWMTMRRGTALDLVVCDLDGCGGPEVAM
                     DVARVLASRWAPGLEMRAHLVDLVAVVNALRERVDSRLRQVLLKRAMYRVATQVAADI
                     GADAIVTGESLGQVSTQTLRNLAVADEAAGLPVLRPLIGLDKEEIIARARQIGSHDAS
                     ARVTEHCAIATGRVETAARLDEVLTAEGDIEETVIEAAVRARRVVDVRRWEPAPPRPE
                     HVVERVPEGAVVVDVREPDEGADVGDLRLPFSRVAEWAGDLDPAREYVFVCSHGVRSE
                     LVARELSSRGLRARSLAGGAKSLGDRAA"
BASE COUNT         2107 a         5235 c         5183 g         2131 t
ORIGIN      
        1 gagtacgaca aggccgaggc gatgctcgag gcctaccgcc acgccaccga gatcgagcgc
       61 aagggcgcgg tgatgttcgg caacgagatg atcgacgagg cgagccgcaa gatggccgag
      121 cagctcgccg cgcgcggcgc cgcggccggc ctgagcccgc agaagacctg ggacgacttc
      181 aaggaggagt ggcaggcgtg atcgagctca ccgaggacca gcaggcgatc gtcgacgcgg
      241 tccgcgagtt cgtcgagcag gaggtcatcc ccgtcgccga cgagatggag caccgcgacg
      301 agttccccga caagatcgtc gagcagctca aagagatggg gctgttcggg ctcacgatcc
      361 ccgaggagtt cggcggctcc ggcatggaca cgatgaccta cgcgctcgtc gtcgccgagc
      421 tctcccgcgg ctggatgtcg atctccggcg tgctcaacac ccacttcatg gccgcctacc
      481 tgcttaacaa gcacggcacc gacgagcaga agcagcgctg gctgccgaag atggcgaccg
      541 gcgagctgcg cgccgcgtac tcgatgtcgg agcccggcgc cggctccgac gtccaggcga
      601 tcaccacccg cgccgagcgc gacggcgacg actacgtcgt gaacggcacc aagatgtggg
      661 tgaccaacgg cctccgcgcc ggcctcgtcg tcacactcgt gaagaccgac cccgacgccg
      721 atccccccgc caaaggcatg tcgctcctct acgtcgagaa ggagccgaac gccgagcgct
      781 tcgagggcat cacggtcccg cccaacatcc agaagatggg ctacaagggc gtcgagacca
      841 ccgagctcgt cttcgagaac caccgcgtcc ccgccgccaa cctcctcggc gaggaggagg
      901 ggaaaggctt ctaccaggtg atggacggga tcgaggtcgg gcgcgtgaac gtttcgggcc
      961 gcgccgtggg gctcgccacg cgcgcgttcg aggaggcgat ccgctacgcc cagacccgcg
     1021 aggcgttcgg caagccgatc gcgaagcacc aggggatcca gttcaagctc gcgcagatgg
     1081 gcacgaagct cgaggcgatg cgcctgatgc tgctctcggc cgcgaagaag aaggcctccg
     1141 gcgagcggtc cgacctcgag gccgggatgg cgaagctctt ctgctcggag atgtgcaagg
     1201 aggtcgtcga ggacgcgctc cgcatccacg gcggctacgg ctacagcgcc gagtacaccg
     1261 tggagcgcct ctaccgcgag gcgccgttcc tgctcatcgg tgagggcacc agcgagatcc
     1321 agcaggtgat catcgcccgc cagctcctcg aacgcttcaa gatccaggcc taggaggccg
     1381 ggcgccagtt ctcgcggtac cagtcggcgg tccggcgcag gccctcctcg aggtccacct
     1441 tcgggtcgaa gccgagcatg cggcggatct tcgagatgtc gacgctcgta cggtaggcct
     1501 caccggggcg gcgcccctcg aagcggatct cgacgtcggc gccgaccacg ttgcggacga
     1561 gctcggccac gtccctgatc ggagtctcga cgcccgtcgc cacctggaac acctcgccgg
     1621 ccagctcgtc ggcgtccatc gcgcgcacga tcgcatcgca gaggtcgtcg acgtagatga
     1681 agtcgcgggt ctggctgccg tcgccgtaga tcgtgatgtc ctcacccgtg aggcacttca
     1741 tgatgaagct cggaacggcg gtccacgtct tgcgggccga gtgggggccg tagacgttgg
     1801 agaaccgcag cgccgccgtg cgcatcccga aggccgacgc gtaggcgtgg cagtaggcct
     1861 cgcccccgag cttcccggcg ccgtaggggg acagcggccg cgggacgacc tcctcgtgga
     1921 tcggcggctc gacgtccccg gcggcagcgc ccgtcgaggc gaagaccagg cgctcgaccc
     1981 cggcccgccg gcaggcctcg aggacgcgga aggtcgtggc gaggttcccc tcgaagtccg
     2041 tgacggggtc ctccaccgaa ggcgccaccc cggtctcgct ggcgaggtga acgaccgcgt
     2101 cgacgccctc gagcgccggg tccatcacgg cagggtcggc gcagtcgccg atcgtcacct
     2161 ccgcggctcc ctctggtatc cgcgagacgt cccccttgcg gaggtcgtcc acgacgcgga
     2221 tctcgaggtc ccgctcggcg aggatccgga cgaggttcgc cccgatgaag ccgcagccac
     2281 cggtgatcag cactcgtcgg acgtcgggca tcgccagcct tccttcggga gggagcggct
     2341 agggtatcat cacgccgttc cgcgaccgga ggcatcctga ccggccccat gaacatcctc
     2401 cgcatcatcg gcttcctcgt cgccctgctc ctcgtggtgc aggcgatccg gcgctaccgt
     2461 cgccgctcgc tccggctgcc cgacacggcg atcatgctgc tgctggcggc cggtctcgcg
     2521 accctgtcga tcgcaccggc cctgatcgac ccggtgctcc gcgtcctcgg cttcccgccg
     2581 gggaacgcgc ggcgggtcat cggcgtcctc gtcgtctcag ggatcctcac gtacatcctg
     2641 ctgttccgct cgttcgcgaa gacggaccgg ctggagcaga ccctcggcac ctacgccgac
     2701 cgcgtcgcgg cacgggagtt catccgcgag tacggccacc cccagccccc ggccgaccgg
     2761 aagaagctgg cggtcgtgat cccggcgctc aacgaggaga cgagcctccc gggggtcatg
     2821 aaggacgtcg cgaccgaggt cgaagggatg gaggtcgaga tcgtcgtcgt ctccgacggc
     2881 tcggtcgaca agacggagca ggtcgccgag gagcacggag cgctcgtcgt gcgtcgggac
     2941 ctgcgacgcg gccagggcgc cgccgtccgc ctcggctacc acatggcgct tcagcgcggg
     3001 gccgacatcg tcgccaccgt cgacgccgac ggccagtacg acccgctcga gcttccgcag
     3061 ctcgtccgtc ccatcctcga cggcgaggcc gacgtggccc acggctcgcg gatgctgggg
     3121 gagtacgcga agccgatctt cggacggtcg cagggggttc gggtgttcgc ctggctcacg
     3181 acccgcatgg cggggcatcg catcaccgat cccgcgagcg ggttccgcgc cttcagcgcc
     3241 gaggccctcc gacagctcga gttccgggag aaccagttcc acgcgagcga ggtcaccgtc
     3301 gcggccgcga aggcgggtct gcgggtgaag gaggtgccgt gcaccttccg cgagcgtcac
     3361 tcggggaaca gcaagaagcc gccgctcctg cgctacggat ggggctatgc ccggagcctg
     3421 atccgcacct ggctcggctg agcctcagac cttctcgcca ggctggacgt ccttcaggag
     3481 gccgtccttc cagttgtggc tcgggtagag gagccggcgg ctgacgcgat cggcgtgctg
     3541 cttcaccgtc gcgagcaggc cgtcgatcag cgtctcggag aggaggtacg gctgaagccc
     3601 gaggtcgagg agcttcgtgt gcttcgcgtt gtagtagtgc tcctcgagct ccacgcgcgg
     3661 gttcggcacg ttctcgatca tcggctccca cccgagtttc cgggcgccgt cggcgacctt
     3721 ctccgcgagc tcgcccaccg agaactgctc ggtgaactgg ttgaagacct cgtaggtccc
     3781 catctcgggc gggttgttgc aggcgatctc gacgcactgc agcgtgtcgc ggatgtcgag
     3841 gaagccgcgc gtctggccgc ccttgccgta gaccgtgatg gggtggtcga tcgccgcctg
     3901 cacgcagaag cggttcagcg ccgtccccca gacgtcgtcg tagtcgaacc gcgtctgcag
     3961 gcgagggtcg agcgccgtct cgtcggtgtg caccccgtag acgacgccct ggttgagatc
     4021 ggtggagcgg aggccccagg cgcggcagac gaactcgatg ttgtgcgagt cgtgcacctt
     4081 cgacaggtgg tagaagctcg gcggcttctt cgggtagagc accacgtcct tgcggccgtt
     4141 gtgctcgatc tcgatgaacc cctcctcgat gtcgatgttc ggcgtcccgt actcgcccat
     4201 cgtcccgagc ttgaccaggt gggcctcggg cgtgaggtcc ttcatcgcgt agaggatgtt
     4261 gagcgtgccc accacgttgt tcacctgcgt ctcgaccgcg tgacggcggg agagcatcga
     4321 gaacggggcg gcgcgatgct cggcgtagtg gacgatcgac tcgggatcga agtccttcca
     4381 gacgtgctcg aggaagtccc actcggtgat gtcgccgatg tacatctcga tccggccccg
     4441 gccgagctcg ttccacaccc ggacgcggtc gtggagcggg gcgatcggga tcagggagtc
     4501 cgcgccgatc tcctggtccc agaggcgccg gtggaagctg tcgaccacgg ccacgtcgtg
     4561 ccccttgttc gacaggtaca tggccgtcgg ccagcccagg tagccgtcac cgcccaggat
     4621 caggatcttc ataggcgtgg atgatatctt ccggcgagcc gccctgacca ccctggagga
     4681 cccgatgggc accgcgcgcg agttcggccg attcctcgag gatttcgagg tcggcgacgt
     4741 ctacaagcac tggccgggca agacgatcac ggaggccgac gaccacctgt tctgcctgat
     4801 cacgatgaac caccacccgc tccacctcga cgcgaactac gccgagacgc agacgcagtt
     4861 caagcggaac gtggtcgtcg ggaacctggt gtactcgctg gccctcggga tgtcggtccc
     4921 ggacgtctcc ggccgggcga tcgcgaacct cgaggtggag tcgctccagc acaaggcgcc
     4981 gatgttccac ggggacacgc tgtacgcgga gacgacggtc gtggacaagc gggggtcgtc
     5041 ctccaagccg gatcggggcg tcgtgaccgt ggagacccgc ggctacaacc aggacgggac
     5101 ggaggtcgtc ttcttccgcc gcaaggtgat gatcccgaag cgcgggtcga ccgaggaaca
     5161 gcccggtcgc ccgacgccgc aggcctagcg tcgcagaggg aagcgcgctt ccgccacggt
     5221 ttccccgtcg atccgcagga tccgcagcgt tccaccggcc gcctcgcaga ggcggctcgc
     5281 gacgaagagc ccgaggccca tcccgccgcg ctcgcgcgtc gtcgacatgt cctcctggac
     5341 gaacggacgg aacagcgccg gatcgacgtc ggtcccgccg gcgtcccgga cccgcaggac
     5401 gccttcgccc ccctcgcggc gggcctcgat cgagaccggc cgccgcccat gccggagggc
     5461 gttcgcggtc agctcgtacg cgacgcgcgt caggtgacgc tcggggatcg tgggccagtc
     5521 ctcgtcccgg agatcgagcc ggtccggcgt ctcctcgagc ttcctgctgg cgtcccgcac
     5581 ggctcgggcg gggttcgcct gggggttccc gtcgcccggg tcgccgatca gctcgagtcc
     5641 ctcgatcagg tgctcgagct ggtcaccgcg gcgcagggcg gagcggacga gcgcggcgac
     5701 gtcctcgcgc ggcacgacct gctccaccgt ccgaagggcg cccgacatca cggtcagtgg
     5761 tgtccggagc tcatgcgcca gcacgtggac gaactcctcg cgcatgcgcg cgagccgctc
     5821 ggcctcgcca cgtcgctgcc gctcctcccc gaggagacgc cggaggtcgt cgagcacgtg
     5881 ggggaggatc cggtcgtcga tcggtccctc gccggcgtgg gcgctgcgga cggctgccgc
     5941 caggtcgtcg atcgccgtcc ccttcacgac gtagcccgcg gcgcccgccg cgagcatctc
     6001 acggaccgtc tcggcatctt cgtgtgccgt cagcgcgatc acgcgcgtgg ccggatgccg
     6061 caggtggatc gcgctcgtcg cctcgacgcc gttcccgtcg ggcatctgga cgtccatgag
     6121 gatgacgtct gggacgaggt cgtcggcctg ccgcagcgcc tgggcgcccg acccggcctc
     6181 gccgacgacg tcgatgcgct cgtcgaagga cagcgcccgc cgcagggccg ctcggctctc
     6241 ggcggcgtcg tcgacgacga gaaccctgat ccgggctgct ccgttcccga ccatcccgtg
     6301 caacctcctg cccctactat cgggccttcc gggcgcacga cggagggggg agtgcggcct
     6361 acccccaccc gggggaccct gcgaggatgg gaggcatgag cgagacactc gatggcatcg
     6421 ccagggaggt cgtgacctgc acgcggtgcc cgcggctcgt ggcctggcgc gaggaggctg
     6481 cgcgccggcc gccgaagcgc tacgaggggg agcgctactg ggcccgcccg ctccccgggt
     6541 tcggcgatcc ccgcgcgcgg atcctggtcg tcggcctcgc cccggcggcg cacggcggga
     6601 accggaccgg gcgcatcttc accggcgacc gctccggtga cttcctcttc gcctcgctcc
     6661 accgcaccgg ctacgccaac cagcccacct cgacgcaccg cgacgacggc ctccgcctgc
     6721 gggacgtcta catcgcggcg gtgaatcgct gcgccccgcc ggggaaccgg ccgacgcccg
     6781 aggagcgcga caactgcctg ccgtacctcg tccgtgagct acgggccctg ccgaacgtcc
     6841 ggtgcatcgt ctgcctcggc atgttcgcct ggaacggcgc gatccgcgcg ctggccgccc
     6901 tcgggcaccc gctgccgagg ccgaggcccg tgttcgggca cgtcgcggac gtccaggtcg
     6961 gtccgtaccg gctcgtcggc tgctaccacc cgagccagca gaacaccttc acgggcgtcc
     7021 tcaccgagca gatgctcgac gacgtcttcg cccgcgcctg ctcctgaggg ggcgttgtcg
     7081 ggggccgggc gtatccttgc tcccgtgagc caccaccgca tgctgatccc ggacccggac
     7141 gctcctcccg tgacgaaggc ttccctccgg cgcgtcgccc gcacgttctc gccgtacagg
     7201 gggcgcatcg cgctcgtcgg gatcgcgatc ctcgtcacct cgacgctcgg ggtcatcaac
     7261 ccgatcctca tcaagatcct gttcgaccgc gcgctcttct gcgccgaggg ctgcgtgaac
     7321 ctcgacctgc tctacgggct cgtcgccctg atggtcgcga tccccgtggt ctcgggcgtg
     7381 atcgggatcg gccagaccta cctctcgaac ctcgtcggtc agcgcgtgat gcaggacctc
     7441 cgcaacgggc tgtacgcgca tctgcagcgg atgtccctgc ggttcttcac ggccacgcgg
     7501 accggggaga tccagtcgcg gctgtcgaac gacgtcgcgg gcgtgcagtc ggtcgtgacc
     7561 gataccgcgt ccagcctgct ggcgaacgtc gtgatcgtga tctcgacggt ggcggcgatg
     7621 gcgatcctct cgtggcagct caccctgctc tcgctcggca tgctgccgtt cttcttctgg
     7681 ctcacgtacc gggtcgggaa ggcacggcgt gaggtcgcgg cctcaacgca gaagagcttc
     7741 gcggacctct cggcgatcac cgaggagacc ttgtcggtct cggggatcct cctgtcgaag
     7801 gcgttcgggc ggcagcagca cgagacggag cgcttccggt cggagaaccg gcgcctcgcc
     7861 ggtctgcaga tccgccagca gatgatcggg cgctcgttct tcgccacgat ccagatcttc
     7921 ttctcggtga cccccgcgct cgtctacctc gtcgccggga tcgtcatcgc ggggaacccc
     7981 gcgggagccg gactcacggc agggacgatc gtggcgttca ccacgctcca gtcgcgcctg
     8041 ttcttccccg tcgggcagat gctccaggtg ggcgtcgagg tgcagagcgc gatggcgttg
     8101 ttcgaccgga tcttcgagta cctcgacatg gagcacgaga tccaggacgc tcccgacgcg
     8161 gtcgaggtgc cgaaggacac ggtcgagggc cgggtccggt tccgcgacgt gagcttccgc
     8221 tacggggagc agtggacgct ccgcgacatc gacctcgaga tcgagccggg ccagctcgcg
     8281 gcgctcgtcg gaccgagcgg cgccgggaag acgacgatga cctacctcgt cccgcgcttc
     8341 tacgacgtcc aggagggcgc ggtcgagatc gacgggaagg acgtgcgggg gatcaagctc
     8401 gagagcctgg gcgagatcgt cggcatggtc acgcaggaga cgtacctgtt ccattcgacg
     8461 gtcgcggaga acctccgcta cgggaacccg gccgccaccg acgccgagct cgagacggcg
     8521 gctcgtgccg ctttcatcca cgaccgcatc gtggagctga cggatggcta cgacaccgtg
     8581 gtcggggaac gcggctacaa gctctccggg ggggagaagc agcgcctcgc gatcgcgcgg
     8641 gtgatcctca aggaccccgc gatcctgatc ctcgacgagg cgacctcctc gctcgacacg
     8701 acctcggagc ggctcgtcca ggaggccctc cgtcccctca tggcggggcg caccacgatc
     8761 gcgatcgccc accgcctgtc gacgatcctg gccgccgacg tcatcttcgt ggtcgaccgg
     8821 ggccggatcg tggagcgagg gacgcacgag gacctgctcc gtcggagcgg gctctacgcg
     8881 cgcctctacc agcagcagtt ccacggcggg gaggtagagg ccgagtgcga ggacggggtc
     8941 gtgctcgcga gcggcgaggt cgttcgccgc tagtgccacc cgagctcgtc gagaccgacg
     9001 acctgacggc cgcggcgctc gcgctgttcg tcgaggcgga ccctcgcagg atcgtcctcg
     9061 ccggcggcga gacgcctcgc cccgtctacg agcgcctgcg cgaggtggat catccctggg
     9121 ccgagaccca cgtgctcatg ggggacgagc gctgcgtgcc gctggatcac cccgcgtcga
     9181 acttcgggat ggcgagggag gcgctcctcg cccacgtccc ggcggtggcc cacccgatgc
     9241 cggtcggctc ctgcgaccct ggcgcctacg aaagcgtcgt ccgggacgcg ctgggcgatc
     9301 ctccgcgggt cgacctcgcg ttcctcggca tcggcgagga cggccacacg gcgtcgctgt
     9361 tccccggcga ccccgccctc gcggaaacgg aacgactcgt cgtccgggtc gaccggccgg
     9421 accatccacg gctcacgctg acgcttccgg tcctgtcgtc ggcgcggctc gccatcttcc
     9481 tggtcgacgg cccgtcgaag ggcgtggcga tgcggcgact gctcgccggg gaggaaattc
     9541 cggcatcgcg cgtgcgggcg gagcgggtcc tcgtgctggc gacgccgcgc gtcacccgag
     9601 ggtgacgccg atcagccggc cgacccagta ggtcacgagc gcggccccgc cgccgatcgc
     9661 gagcatgcgg agcccggagg tcagccaggg ccgacccgtg aaccgcgtca tcgccgctcc
     9721 gagcgtgaag agggcggcga gggagaggac gatcgccgcg accgtggccg cgaacccgga
     9781 tgcgacgagg aacggcagca gcggcgtgaa cgctccgagg gagaacgccg tgaacgagga
     9841 gatcgccgcc ccccatgggg atccgagctc ggtggggtcg agcccgagct cctccttcgc
     9901 gtgcacctcg agggcgacgc gggggtcctt catcagcgcc gcagccgtct ccgacgcgag
     9961 gtccggtggg atgcctcgcg cccgcaggat ctccgccagc tcgtcgcgct ccccgtccgg
    10021 gtccatcgcc agctcgtgcg cctcgaggtg gatcagccgc tcgaacagct cgcgctgaac
    10081 gcgcatcgag atgtactcgc ccgcgcccat cgagaaggct cccgcgagga gcccggcgac
    10141 cccggcgagg acgatcacct ccggggcgag gccggcgccc gcgacgccca tgatgagcga
    10201 gaggttcgag acgagcccgt cgttcgcccc gaagatcgcc gcccggagcg cgccgctgcg
    10261 cccgccgatg atgtgctcga tctccccctc cgccgggacc gggccctgat ggggcacgat
    10321 ctcgggcgcg cgccggtcgg tggacgccct catgcgtcgc cgtcgttgcg ctgctcgcgc
    10381 aggaagcgtt cgagctctgc tgcgagcgcc tcgccgctcg ggacctcgaa gtccgtcccg
    10441 ccgacgccct cctcgaggcg ctggacgtac gcctggagct ccgcgttctc ctcgatcatc
    10501 gtcgcgacct gggcctccca cgtagcggcg gcccgcgcga gcgtgtcggt ctcgaccgtc
    10561 gtgccgaggt agaccgacag cttctggacg agggcgaggg ccgccttcgg gttcgtgccc
    10621 gccgggaggt agtgcgggac cgccgcccac aacgagacgg agtcgagccc cgcgcgtgcc
    10681 gccgcgtcct ggagcacccc gacgatgccg gtcgggccct cgtaccggga tgggacgacg
    10741 ccgagacgcg ccgccgtctc ctcgtcggcc gcggtcccgg cgacgggggg cgggacgctg
    10801 tgcgggacgt ccgcgaggaa ggcgcccagc gtgaccaggc tcctcgcccc gagcgcctgc
    10861 gccgtctcga cgatcgtcga cgagaagctc cgccaccgca cgttcggctc gatcccgagc
    10921 aggaccgcca tctcgcgctc ggggagcgaa gcgtggaaga agtcgttctc cggccactcg
    10981 atccggcgcg tgacgccgcc ctcgagtcgg acggtcggcc gggtcacctg gaagtcgtag
    11041 aacggctcgg gatcgatgcc ggcgaagcgg cgggcccccc accggtcgcg caggtacgcg
    11101 gccgcggtcg tggccgcgtc gcccccgtcg ttccacccgt ggaaggcaca gagcagggtc
    11161 ccgccgtcga ggtgggggcg ccactcgaag cgcaccgcgt cggtcatgcc tccatcctcc
    11221 cccggtcggg cccgggtgtc atcctcgggc gccggcgcgg atctcctcgc ggacccgctc
    11281 cgtcagccgg tcgagagcct cggtgcggcg aaccgccagc gggtcggcgt cgagcgctcg
    11341 gagcgcttgc gggagccagt cgcgctcacg gcggaaccgg tcgcgtgcgg cggcgaggct
    11401 gtggtcgccg acgatcgcgc cgtcccgcat cacctcgacc agcagcggct cacctcccgt
    11461 cggggccggc tcgtgcatca gtccgacgac gtccccgccg gaagtccgcc agacctgctt
    11521 gcgccccgga agggtggcct tccccgtcga gagcttcacc accggccgcc cctcgtactc
    11581 gacgagcttg tagacgctgt cgaggtacgg cgcgtccgac gcgaccccga ccttcgaccc
    11641 gatgccgaag gcgtcgatcg gggccgacgc gaggtccgcg acgcgatgct cgtcgagccc
    11701 gccgctggcg atgatccgga cctcgcgcag gccggccgcg tcgagcgcct cgcgcgcgtc
    11761 cctggccagc acggccaggt ccccgctgtc gagacggatc gcggcgagtc gcaggcctcg
    11821 gccttcgagc tcgcggccga cctcgatcgc ggtccggacg ccgcgcgcgg tgtcgtaggt
    11881 gtcgacgagc agcacgaccg agtcggggaa gtcctccgcg aacgctcgga acgccgcttc
    11941 ctccgacacg aacgcctcga tgtaggagtg agccatcgtc ccggtcgccc gcaggccgag
    12001 gagccgcgct gcctcgacgt tgctcgtggc ggcgaacccg gcgatcgccg tggcgcgagc
    12061 gaccgccatc cccgcgtcca ccccgtgcac gcgtcggaag gagaaatcga ccatctcgcg
    12121 gtccggcgcc gccaggacgc atctcgcggc cttcgacgcc acggcggtct ggtaggtgat
    12181 ctggttcagc aggaaggact ccgcgagctg cgcctcgggg aggggagcgg tgacctccag
    12241 caggggggcg ttcgggaaga cgacccggcc ctcgggaacg gcccggacct ctccggtgaa
    12301 gcggaggtcg cggaaccggt cgaggacgtc gtcggggtag ccgtgcgtcc cgcggaggta
    12361 ggcgagatcc tcctcctcga agcggaggtc gcggagccac tcgagcgcgg gcccgagccc
    12421 ggaggcgacg aagaagcccc agccgcgcgg gagcccgcgg atgaacaggc tgaaggtcgc
    12481 ggggcccgtc atgccgcgct ggaggtagct cgcggccatg ttcagctcat agaggtccgt
    12541 gagcagcgcc ctccccatgc gggcgaggat accgcctgcc gtagatggca ggatgtgagc
    12601 gtgccgggtg agcgcaggaa gccaccgccg aagcgcgagc gtccgcgcga cgagctcggg
    12661 cgtccgctcc cgtggggttc ggagaaccgc ctgcagatgg aggacttcga cgcgctgccg
    12721 gtggccgaga accatcggct cgggatcgaa cacttcaacg cgcgccgctt cttccccgcg
    12781 cacgaggcgt gggagactgc ctggaagcag tcgcgaggga cgccggacga ggagttctac
    12841 aaggggctct cccagctcgg cgcggggtac gtccacttct cccgcggcaa cctccacggc
    12901 acgcacacgc tgatccggcg cgcgacgagc cgcctgcgcc ggtacgcgcc cgtccacggg
    12961 cgcatcgacc tcgcggcgct cgtcgagcag gtcgaggagc aggcggcgac cgtcgaacgc
    13021 tcggccgcct cgggggaggg gatgccgccg ctcgaccccc cacggatcgc agcgggtacc
    13081 atcgacccgt gacccgtcgc ctcatggtga agatcgccgg cgagatcggc acgaagtcgc
    13141 cgcgcacccg ccgccggttc ctgcggatcc tcgccgggaa cgtccgtgcg gcgctcgatg
    13201 tggccggggt ggagggggac gtcgagcagc ggtggagccg cctgttcgtc acgacggacc
    13261 gtccgacgga ggcgcgggag gcgctcgccg gcgtattcgg gatccactcg atcagcgagg
    13321 tcgccgtggt tcggttccgc gacctcgacg atctcgtcgc gagcctgagc gacctctacc
    13381 gggaccgcgt aacgggccgg acgttcgcgg tccgcgcgcg acgctccggc gagccgtcgt
    13441 tccgctccgg cgacctcgcg gtggcgctcg gcagcgccct gctcccgcac agcgccggcg
    13501 tcaacctcga cgacccggat gtggaggtgc cggtggaggt cgtacggggg atggcctacg
    13561 cggtcctcgg ggtgacgccg ggagcgaagg ggctccccct cggcagcggc catcgcgcgg
    13621 cggcgctgtt ctccggcggg ttcgactcgc ccgtcgcggc gtggatgacg atgcgtcgcg
    13681 ggaccgcgct cgacctcgtc gtctgcgacc tcgacgggtg cggcgggccc gaggtcgcga
    13741 tggacgtcgc ccgggtgctc gcgagccggt gggcgcccgg cctcgagatg cgcgcccacc
    13801 tcgtcgacct cgtcgcggtc gtcaacgctc tccgtgagcg ggtcgactcc cgcctgcgtc
    13861 aggtgctgct caagcgcgcg atgtaccgcg tcgcgaccca ggtcgccgcc gatatcggcg
    13921 ccgacgccat cgtcacgggc gagtcgctcg gacaggtctc gacccagacc ctgcgcaacc
    13981 tcgccgtggc ggacgaggct gccggactcc cggtcctgcg cccgctgatc gggctcgaca
    14041 aggaggagat catcgcgcgg gcccggcaga tcggctccca cgacgcctcc gcgcgcgtca
    14101 ccgagcactg cgccatcgcg accggccgtg tcgagacggc ggcgcgcctc gacgaggtgc
    14161 tgacggcgga gggcgacatc gaagagacgg tcatcgaggc cgccgtccgc gcgcgacggg
    14221 tggtcgacgt ccggcggtgg gagccggccc cgccccggcc cgagcacgtc gtcgagcggg
    14281 tgccggaggg cgcggtcgtg gtcgacgtcc gggagccgga cgaaggcgcc gacgtcgggg
    14341 acctccgtct gccgttcagc cgcgtcgccg aatgggccgg cgacctcgac cccgcccgcg
    14401 agtacgtctt cgtctgctcg cacggggtgc gctcggagct cgtcgcgcgg gagctctcca
    14461 gccgcggtct gcgggctcgg tcgctggcgg gcggcgcgaa gagcctcggc gaccgcgcgg
    14521 cctgatcgga tgccggtcct cggcccgtcg tcgaaggggg tcgtcgtcgc gacctcggat
    14581 ccgcgcgcgg cgctcgagcg gcgggacgcg ctgcgcggcg agggcctgcc ggcggcgacg
    14641 ctcgacgagc tcggcc
//
LOCUS       DATLGG010000314         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_15453, whole genome shotgun sequence.
ACCESSION   DATLGG010000314 DATLGG010000000
VERSION     DATLGG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15453"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VM638_08865"
     CDS             complement(<1..714)
                     /locus_tag="VM638_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12567.1"
                     /translation="MTTRWRWGAAALAAYGAAALGLGLSGARLLPVFDGVAPVAPYRW
                     VNPPPEMAQGNQPPESGEQVIEADADVKALAAFTLDAQASMTAADPAFGEHPDERSVR
                     VTVRPLDPETLGPPPEGLGYQGNAYEFAATFQPSGEPARLEQVATVVLRYPIHATTVL
                     RKVDGGWERLPKPITVGESLTIYAETPEMGTFVAAGPPTEEEKAGVLRWVGYGAAGLA
                     LVGAVALALIRRRRASAARR"
     gene            complement(711..1946)
                     /locus_tag="VM638_08870"
     CDS             complement(711..1946)
                     /locus_tag="VM638_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVM12568.1"
                     /translation="MRKVLIVVAGLALAAQGCTASTAALVVGAVYPTGGGQGEEAIEE
                     YRGVLMAAERVNAAGGIGGRRVEVVLERAETADAAPGAVRRLADRGIELVVGSYGSTI
                     SAPAAAEAARQGMVFWETGAVGEMTPDAKPGERFFRMAATGGTLGRAGVRFVADRLLP
                     ELGVDGNVRYAVAYVDDVYGRSVGLGAIEEIESSGLTLAGTYPYDLATLDVDDLARRM
                     DAAGTEVLVVAAYLEDGVDLRRAVLERGIDLAASIGTSSSYCMPDFGIALGEDAVGLF
                     ASDKPEADFLQPEMLSEEAGKALRWVRTEYRRRHGEAMSGPGLAGFAGALALFEHVLP
                     RAGSDDPDAVADAARQTRLPLGALPNGSGMRFAPEGHAEAGANLHAASVIWEWVAPEQ
                     REVVWPPALATAPILAVRP"
     gene            complement(1943..>2169)
                     /locus_tag="VM638_08875"
     CDS             complement(1943..>2169)
                     /locus_tag="VM638_08875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HVM12569.1"
                     /translation="FLYGFLSRWGPALAVLGSAAAFALAHAPRYGWRAVSLDLGAGVV
                     FGWQRWATGRWTSPAATHVVANLMGMGVLG"
BASE COUNT          295 a          862 c          749 g          263 t
ORIGIN      
        1 acggcgggcg gccgatgcac gacgccggcg gatgagcgcg agggccacgg cgcccacgag
       61 cgcgagcccc gccgcgccgt acccgaccca ccggagcacc ccggctttct cctcctcggt
      121 cggcggcccc gcggcgacga aggtccccat ctccggggtc tccgcgtaga tcgtgagcga
      181 ttcacccacc gtgatcggct tcggcaaccg ctcccaccct ccgtcgacct tccgcaggac
      241 ggttgtcgcg tggatcgggt agcggaggac gacggtcgcc acctgctcga gccgagccgg
      301 ctcgcccgac ggctggaagg tcgccgcgaa ctcgtaggcg ttgccctggt agccgagccc
      361 ctcgggcggc gggccgagcg tctccgggtc aaggggccga acggtgacgc ggacggaccg
      421 ctcgtccgga tgctcgccga acgcggggtc ggcagcggtc atcgacgcct gggcgtcgag
      481 ggtgaatgcg gcgagcgcct tcacgtcggc gtccgcctcg atcacctgct caccggattc
      541 cgggggctgg ttcccctgcg ccatctccgg cggtgggttc acccagcggt agggagccac
      601 cggggccacg ccgtcgaaga cggggagcag acgcgcgccg gacagcccga gtccgagcgc
      661 ggccgcgccg tacgcggcga gcgcggccgc gccccagcgc cagcgagtgg tcacggtcgc
      721 accgcgagga tcggcgccgt cgccagcgcc ggcggccaga ccacctcgcg ctgctccggt
      781 gcgacccact cccagatcac gctggccgcg tgcaggttcg cgcccgcctc cgcgtgtccc
      841 tcaggggcga agcgcatccc cgagccgttc gggagagccc cgagggggag gcgcgtctgc
      901 ctcgccgcat ccgcgacggc atccgggtcg tcgctgcccg ctcgcggcag gacgtgctcg
      961 aacagcgcca gcgctccggc gaagcccgcg agcccgggcc cgctcatcgc ctcgccgtga
     1021 cggcgacggt actcggtgcg gacccagcgg agggccttgc cggcttcttc ggagagcatc
     1081 tccggctgga ggaagtcggc ctccggcttg tcggaggcga agagcccgac cgcgtcctcc
     1141 ccgagcgcga tgccgaagtc gggcatgcag tagctcgagc tggttccgat gctcgccgcg
     1201 aggtcgatcc ctcgctcgag gaccgcgcgg cgcaggtcga cgccgtcctc gaggtacgcc
     1261 gcgacgacga ggacctccgt ccccgcggcg tccatgcgcc gggcgaggtc atcgacgtcg
     1321 agcgtggcca ggtcgtacgg gtacgtcccg gcgagggtca agcccgacga ctcgatctcc
     1381 tcgatggcac cgaggcccac cgagcggccg tagacgtcgt ccacgtaggc cacggcgtag
     1441 cgcacgttgc cgtcgacgcc cagctccggg agcagccggt cggcgacgaa ccgcacaccg
     1501 gcacgcccga gggtcccgcc ggtcgccgcc atccggaaga agcgctcccc tggcttcgcg
     1561 tccggcgtca tctcgcccac cgcgcccgtc tcccagaaga ccatcccctg ccgggccgcc
     1621 tcggctgcgg ctggggccga gatcgtgctg ccgtagctgc ccacgaccag ctcgatcccg
     1681 cggtccgcga gccgccggac ggcgcccggc gcggcgtcgg ccgtctccgc ccgctcgagc
     1741 acgacctcga cgcggcgtcc gccgatgcct cccgcggcgt tcacccgctc ggcagccatg
     1801 agcaccccgc ggtactcctc gatcgcctcc tcgccctggc cgcctcccgt cgggtacacg
     1861 gccccgacga cgagcgcggc ggtggacgcc gtgcatccct gcgccgccag cgccaatccc
     1921 gcgacgacga tcagcacctt cctcatccga gcacccccat ccccatcagg ttcgccacca
     1981 cgtgcgtcgc ggcgggcgag gtccatcgcc ccgtcgccca ccgctgccaa ccgaacacga
     2041 cccccgctcc gagatccagc gacaccgccc gccagccgta ccgcggcgcg tgcgcgagcg
     2101 cgaacgccgc cgccgagccg aggacggcga gcgccggtcc ccagcgggaa aggaagccgt
     2161 agaggaaac
//
LOCUS       DATLGG010000315        15213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_107428, whole genome shotgun sequence.
ACCESSION   DATLGG010000315 DATLGG010000000
VERSION     DATLGG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107428"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..2662
                     /locus_tag="VM638_08880"
     CDS             242..2662
                     /locus_tag="VM638_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVM12570.1"
                     /translation="MRTSMRCGTLSLLLVAALAPGAAGAGGGAKPATYEVGWRRGGFS
                     APYELAAFEHPSGPAIFVGGYTGGVAAVGRRGVLWRWATGNTQVASIATGDLTGDGIA
                     DLVAALYDGRRLVALDGVDGSSLATRSFPTVPEGIAVADADGDGAGDVYVVTQGFSDH
                     ANTVHLLTGPQLRPTWSASVGSPQGSVLGIDAQDVTGDGRADIGFGTTSNTPRVYLFD
                     ASGAKRWEADLGGTVTNVAIADGGRTIFATRGGGLLAALDAASGASRWSLPSALAASR
                     LALGDLDGDGEDDAVWSVFGPSGKSPTFQVVAVSGATGKPLWWQTTVTPAKAIVVGDF
                     DGDADPDVAVSTQDAGSGTEVVNSVIAYEGGTGRPLWTHPFAGEQTTFLSDVVLADMA
                     GDSRPEVIVAPYWEVLHGLDGAAGGATWTSALGSGVTSAAVADLDGDGAPEVIHGDRD
                     FRVSARDAATGTVRWTRDLGGDVVHVSPLPHDGGHDVLAIGQSTLYRLRGRDGAARWS
                     LPLQGMGTEIERITATSGEVFLAVGARARRMQLGQPGNSLLGYLNLVDAATGRLHWTV
                     PSPGVPWYLDVADLNGDGARDLAVGMTNANLGVAVYDGAALASAPVALWRSATDGVPT
                     SLTVIGRDVVTSRGSVNAVVAQDGATGQERWRRELGSTTRLVGVDDVNDDGTADMLVA
                     TQLFADRIAAVSGADGTVVFDIAAGIRFVTAARSTDLSGDGLADLLFTSDGGTYGDGG
                     VFALDGRTLTGPTPQRLWSYPDVNATALLPVSLERGEHWLAYGFTVRPDALTLLRPIP
                     EEPDPADD"
     gene            complement(2693..4615)
                     /locus_tag="VM638_08885"
     CDS             complement(2693..4615)
                     /locus_tag="VM638_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HVM12571.1"
                     /translation="MTPAAAAPILDGLNPAQREAVTATRGPVAIIAGAGTGKTTTITR
                     RIAYQVATGSFRPSEILAVTFTEKAAGELKARLTRLGVEGVEARTFHAAALSQLSRLW
                     EPSTGTPLGEVLDAKAPLIASMANALPPPHRFVPRRELATEIEWAKNRLVAPERYAEE
                     LRRTGHQPPIPPDLMERVHAGYERRKRATGRFDFEDMLARALQLFDDHAEAADAVRGR
                     YRAFTVDEYQDVNPLQQALLDRWLGDRDDLCVVGDDHQTIYGFTGATPSYLLRFPDRF
                     PGAKVVTLEESYRSTPQVLAVANRLGSRLGGYRKTLRTARPAGPPPLIRPLAGEAAET
                     AHVVETVRRLRTEGIPLEEIAVLYRINARSEPFEEALAAQGIAFQVRDGAFLRRPGPR
                     SALARLRRTGGEVVEAVDGVTAALGLDAPGEADSEEEVTRRADLARLRTLAREFAEAR
                     PEDDVAAFLDELSRRFSTEREGRGVNLMTYHRAKGLEFEAVLLPRLLDGELPFRSGRH
                     RAPVDEERRLLYVGITRAKRILHLTWPTDARSSRSPFLDELGAEGAPVRGAEQPAAAK
                     EDDPLFEALRRWRKERAARDGVPAYVVFHDATLADIAARRPRSLPDLRSISGVGPAKI
                     ERYGPEVLAVVGGSSG"
     gene            complement(4645..5394)
                     /locus_tag="VM638_08890"
     CDS             complement(4645..5394)
                     /locus_tag="VM638_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-type lectin-domain containing protein"
                     /protein_id="HVM12572.1"
                     /translation="MKTSLMTGRRGGRTVVVTVMGAILGLAAAPASAGSVAFPDFSNV
                     GGLRLNGSAQAEGGVLRLTQNSGDQAGSAFSEAKVVNPSKSIRTKFRFRIHDSGDPAD
                     GLAFVIHRDPRGAEALGSNGHHLGYGEREDEQVRIRRSVAVEFNLHYGRSQGRVRVRT
                     NGRTDRAPLASAGMNMYDRDWRVWIAYSPNTERLKVFVAQSDTKPDRPRLVTKVDLAE
                     VLGGTGHAGFTAATGGAAAVHDIQSWRMRNP"
     gene            complement(5492..6055)
                     /locus_tag="VM638_08895"
     CDS             complement(5492..6055)
                     /locus_tag="VM638_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HVM12573.1"
                     /translation="MTALYTVGHSTHGIDAFIDLVRQAGLRHVVDVRVVPASRRHPHF
                     ARDALERALASAGLGYTWEGEALGGFRKAPPGSRHTALRNDSFRGYAEHMGTVAFRSA
                     LGRVLERAAEEPTTVMCAEAVWWRCHRGLIADAVVAQGHAARHLMPDGSIRAHALRDI
                     VRVEDGWPVYDVVPIQPAFEDLQGHTP"
     gene            complement(6069..6800)
                     /locus_tag="VM638_08900"
     CDS             complement(6069..6800)
                     /locus_tag="VM638_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVM12574.1"
                     /translation="MADIRDQIREFWDHDAETYDQSPSHALTDPVEAAAWRALLRRHL
                     PPPPAHVLDAGAGTGAITALLLDLGYRATALDISSAMLDRARTKLAGRDVEFVVAPAD
                     EPPPGPFDAVVERHVLWTNPDPVRTLAAWREVAPEGRLVAMEGLGPSTIERRVRFAFA
                     SLVRRVLDVPHDHHDHYGEDVVRALPLLHARTPAPLLGAARRAGWKGRRVERLRDVEW
                     ARRMIAPTLISRLESTPLFAVVADA"
     gene            6882..7844
                     /locus_tag="VM638_08905"
     CDS             6882..7844
                     /locus_tag="VM638_08905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HVM12575.1"
                     /translation="MIPPPIPSDEQDRLATLHALQILDTEPEERFDRVTRLAQRLFDV
                     PIALVSLIDAERQWFKSKIGLEASETSREVSFCAHAIAGEGILHVPDAREDPRFHDNP
                     DVLGGANIRFYAGCPIQAPDGRSMGTLCIVDDQPRHVDEEALSSLRDLAEMVEQELAA
                     LALAVQDELTGLANRRGFVGLAAQVLAVAMRQGLPATLAYIDLDGFKAINDTHGHAVG
                     DRALQDVARILTESCRSSDVLGRLGGDEFALLFLAEESAAPLDRLHDAVARHNDLAGR
                     PYRLQLSVGTARFDPEEGVGLEELMERADRSMYERKARRQRASA"
     gene            complement(7851..9263)
                     /gene="sufB"
                     /locus_tag="VM638_08910"
     CDS             complement(7851..9263)
                     /gene="sufB"
                     /locus_tag="VM638_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HVM12576.1"
                     /translation="MAKTQDEQLKDLAQGYRFGWSDPAHHVFEPKRGLSERVVEEISF
                     MKSEPEWMRKFRLKSLRHFDRRPMPWWGADLSEIDFNNIYYFIRSTEKQAKEWEDLPD
                     DIKSTWDKLGIPEAEKKFLGGVSAQYESEVVYHKIKDELDKLGVIFTDMDSALRDHPD
                     LVKEYFGTVIPPNDNKFAALNSAVWSGGSFIYVPPGVHVDIPLQAYFRINAENMGQFE
                     RTLIIADEGSYVHYVEGCSAPIYTTDSLHSAVVEIIAKPTARVRYTTIQNWSTNVYNL
                     VTKRAVAHEDAVVEWIDGNIGSKVTMKYPSIYLMGKGARGEVLSVAYAGPGMHTDAGG
                     KAIHVAPHTTSIINSKSVSKGGGRTGYRGLIRVEPEAHHAKSSVRCDALILDEESRSD
                     TYPYIEIEEETAAIGHEATVSKVGDDQLFYLMSRGMSEADAMAMIVNGFIEPITKELP
                     MEYAVELNRLIELNMEGSVG"
     gene            complement(9313..9627)
                     /locus_tag="VM638_08915"
     CDS             complement(9313..9627)
                     /locus_tag="VM638_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HVM12577.1"
                     /translation="MSILSYLRFALTEDADLEAFAGDIEAMRILAAEQPGYRWAEVGQ
                     DPRDPRVWVVVSEWDTVEQVRAWEHHPEHERLYDPWEPHYREPFMHRRFSPWVRPAVP
                     PG"
     gene            complement(9632..9994)
                     /locus_tag="VM638_08920"
     CDS             complement(9632..9994)
                     /locus_tag="VM638_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HVM12578.1"
                     /translation="MEEPNGDFVSDVTTLPGEGGDEETRKRVVQALAKVNDPEIGINI
                     VDLGLVYDLTIEEGTVKVTYTLTTMGCPIGPMIEEQMRGLVDDIDGVKDFEAQMVFSP
                     PWTPEKMSEEAKAALGMF"
     gene            10055..10681
                     /locus_tag="VM638_08925"
     CDS             10055..10681
                     /locus_tag="VM638_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVM12579.1"
                     /translation="MRVLIVDDHPVTRDGLRAALGTAEDVDIVGEASSGDDAVKAVDD
                     LRPEVVFMDVRMPGMNGIEATRLIREEHPETKVILFTVDESRASISEAIQAGVSGYLL
                     KDVGVDELVNAARLALEGKAVIHPTLTQSFIEEVRLVERKPEAPLSRREIEILQKVAY
                     GATTKEAADQLGISPHTVKTHLERIFEKLGANDRAQAVAIAIRRGIVE"
     gene            10728..12002
                     /locus_tag="VM638_08930"
     CDS             10728..12002
                     /locus_tag="VM638_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVM12580.1"
                     /translation="MSSVVVCRAPGTLADADRAFAGETLEPVDDGYRAVEVAQRTRAD
                     LVVVDLGVAALSPGELIKRLRSSNEEVRVIFVLARPAVEEAADVLRAGADGVLAPRPS
                     ADALRWAADEARAGGCPISPSLARGLAERFADIVHREQQWSKTLADVSRQAEELAHTK
                     AEFLSNVSHELRTPLTIIKGVAQVVGRFGNADEQQAGMLAQLEEAATKLTRMVENLLV
                     IAEMERGDFRLDLADHDLAELVRTSAEAAAVRYPAVTLDLRIPPFLRATLDAERMGEV
                     IRHLVDNACRYSDEGDTVTVQARKASEGIVITVTDQGHGVDRKVVSAAFGEAFSPGES
                     VLTKEKAGLGLGLNLARSVVAQHGGILSADPLPGGGSRISVILPPEVKPQAAPAAATN
                     GDHDQQDEAPEDPMALLRELQRKLSDSESRVQ"
     gene            12042..12710
                     /locus_tag="VM638_08935"
     CDS             12042..12710
                     /locus_tag="VM638_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12581.1"
                     /translation="MRRSGIILSFIVLAFAAGPTGPSLDTPPAEGSEVRLVRIDRVSA
                     VLPTDWGVEPLADEGPRRGLRASSDLEAWTTGASRGLEAYWVDATGVGVPSDYYYLVA
                     SGPGATRMPHPRNCRTDDERVLLDARPRPQADVARSGSFVAISTGRCGGSADRTRWVS
                     FVAAPGFGPVGEIGIRESGLYYGLAVVEDGADAAREAKRLLAEVRFDTTSVRRIIEAA
                     GASL"
     gene            complement(12712..13806)
                     /gene="gcvT"
                     /locus_tag="VM638_08940"
     CDS             complement(12712..13806)
                     /gene="gcvT"
                     /locus_tag="VM638_08940"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HVM12582.1"
                     /translation="MDLVRTPLEEEHRGLGAKMGAFAGWAMPIEYQGTLAEHRAVREA
                     VGIFDLTHLGKVDVAGAGALGWLQTVLTNDLSKVGVGQAQYNLVLNESGGIIDDLIAY
                     RVTEDRWLVVPNAANAAKVLAVLQEEERQDVVVTHRPDLTTIAVQGPRALELVAGMFP
                     EVRELAYMECREVSYRDVLVLLARSGYTGEPGVELFAPEGIAPALWREVLARGETHGI
                     VPCGLGARDTLRLEMGYPLHGNDISEERTPLEAALSWSVALDKGPFRGREALLRQKEE
                     GIPSRLWALRMRDRLIPRAHYPVFADGEQVGETTSGTFSPTLRVGIAMAYLSPRERFS
                     VGSTVEVEVRGRRGEAEIVKPPFVASSPKT"
     gene            13896..>15213
                     /gene="lpdA"
                     /locus_tag="VM638_08945"
     CDS             13896..>15213
                     /gene="lpdA"
                     /locus_tag="VM638_08945"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVM12583.1"
                     /translation="MPQDPYDVIVIGAGTGGYSCALRAAQLGLSTALVERDERLGGTC
                     LLRGCIPTKALLQSASVLDHVNRSDEWGIKASGEPDWSAIQGFKSKIVDKLVSGLTGL
                     VKMRKIEVIRGTATITAPGTVEVEGAGTLQARNIVVATGSYPKLLPGLEVGERIITSD
                     QALELDRIPASAVVIGAGAVGLEFASLYRSFGADVTVIEALPAIAPLEDPDISKEMAR
                     AFRKRGIATHAGAKVSEVKETGDGVSVAFESGGKGETVSAELCLVAVGRGPISQGVGL
                     ENVGVNVNERGYVEVDDMLRTSVEGIWAVGDVASTPFQLAHVSFAEGIAVAERVAGND
                     VPAIDYAGVPRVTYSAPEIASVGLTEQQAKDRGHDVVTEKLSMQGIGKANMLGEGGTV
                     KLVAEKDGPVLGVHMLGPHVTDLISEGMLITNWEALPTEVAALIHPH"
BASE COUNT         2187 a         5375 c         5404 g         2247 t
ORIGIN      
        1 tcacggcgga gggcgcgcgc gtgctcgggc tcgccacggg gcgtcggcag cggcgcgatc
       61 gttcctcctg tcatcgtcag acgaggctcc tccggggggg tccaacgtcc agtctagcgg
      121 cgtggagcgc cgaaagtcca tcgcccaggt cggatttggg cgcgtctccc acggggaggg
      181 ccaggctgat ttaggcaatc gcacaaaggg gatcgagccc cccgcggcga accttcgccg
      241 catgagaacg tcgatgcgat gcgggaccct gtcgctgctg ctcgtggcgg ccctcgctcc
      301 gggagccgcg ggcgcaggcg gcggcgcgaa gccggccacc tacgaggtgg ggtggcgccg
      361 aggtggcttc tccgccccct atgagctcgc cgccttcgag catcccagcg gcccggcgat
      421 cttcgtcggg gggtacacgg gcggcgtcgc ggcggttggc cgacgagggg tcctctggcg
      481 gtgggcgaca gggaacaccc aggtcgccag catcgccacg ggcgatctga cgggcgacgg
      541 gatcgcggat ctcgtcgcgg ccctgtacga cggtcggcgg ctggtcgcgc tcgacggcgt
      601 cgacggctcg tccctcgcaa cccgctcgtt cccgacggtc ccggagggga tcgcggtggc
      661 tgacgccgac ggcgacgggg ccggcgacgt ctacgtggtg acccaggggt tctcggacca
      721 cgcgaacacc gtgcacctcc tcaccggacc ccagctgcgt ccaacctggt cggcgtcggt
      781 cggctccccg cagggttccg tcctcggcat cgatgcccag gacgtgaccg gcgacgggcg
      841 tgccgacatc gggttcggga ccaccagcaa caccccccgc gtctacctct tcgatgcgag
      901 cggcgcgaag cgatgggagg cggacctcgg cggcacggtc acgaacgtcg cgatcgccga
      961 tgggggccgc acgatcttcg cgacccgcgg cggcggcctg ctcgccgcgc tcgacgcggc
     1021 cagcggtgcg tcccgctggt cgctcccgtc cgcgctggcc gcctcgcgcc tcgccttggg
     1081 agacctcgac ggcgacggcg aggacgacgc ggtctggagc gtgttcgggc cgagcgggaa
     1141 gtcgccgacc ttccaggtgg tggcggtgag cggcgcaacc ggaaaaccgc tctggtggca
     1201 gacgacggtc acgccggcga aggcgatcgt cgttggcgac ttcgacggcg acgcggatcc
     1261 ggacgtggcc gtgagcacgc aggatgcggg gtcgggaacc gaagtggtca actccgtcat
     1321 cgcttacgag ggcggaacgg ggcggcccct gtggacccac ccgttcgccg gtgagcaaac
     1381 gaccttcctg tccgacgtcg tcctggcgga catggcgggc gacagccgcc cggaagtcat
     1441 cgttgccccg tactgggagg tcctccacgg cctcgacgga gcggccggcg gagcgacatg
     1501 gacgtctgcg ctcgggtcgg gtgtcacatc ggccgcggtg gcggatctcg atggtgacgg
     1561 cgcgcccgag gtgatccacg gagaccggga tttccgtgtg agcgcccgcg atgcggcgac
     1621 cgggacggtg cgatggaccc gcgacctcgg cggcgacgtg gtccacgtct cacctctccc
     1681 ccacgacggc ggccacgacg tcctggcgat cgggcagagc accctctacc gtctccgagg
     1741 gcgcgacggc gccgcgcgat ggtccctgcc cctccagggg atggggaccg agatcgagcg
     1801 catcacggcg acgtctggtg aagtcttcct ggccgtcgga gcgcgggccc ggcggatgca
     1861 gctgggacag ccgggcaact ccctgctcgg atacctgaac ctggtcgacg cggcgacggg
     1921 tcggctccac tggacggtcc cgagcccggg cgtgccctgg tacctcgacg tcgcggacct
     1981 gaacggtgac ggggcgcggg acctggcggt cggcatgacc aacgcgaatc tgggggtcgc
     2041 ggtgtacgac ggcgcggcgc ttgcgagcgc gcccgtcgca ctgtggcgtt ccgcgacgga
     2101 cggggtcccg acgtcgctca cggtcatcgg ccgcgacgtg gtgacgagcc gggggtccgt
     2161 gaacgcggtc gtcgcgcagg atggagcgac cgggcaggag cggtggcgca gggagctcgg
     2221 ctcgacgacg aggctggtcg gtgtcgacga cgtgaacgac gatggaacgg cggacatgct
     2281 ggtggcgacc cagctcttcg cggaccggat cgctgccgtc agcggggcag acggaaccgt
     2341 cgtgttcgac atcgctgcgg ggatccgatt cgtgacggct gctcggtcca ccgacctctc
     2401 cggcgatggg ctcgcagacc tgctcttcac ctccgacggc ggaacgtacg gggacggcgg
     2461 cgtcttcgcc ctcgacggcc ggaccctgac gggcccaacc ccgcagcgtc tgtggtcgta
     2521 ccccgacgtg aacgcgacgg cgctcctgcc ggtgagtctc gagcgtggcg agcactggct
     2581 cgcctacggg ttcaccgttc gccccgacgc gctcaccctc cttcgaccga tcccggaaga
     2641 gcccgacccg gccgacgact gacccgccgc gcgccgcggg tcatcaccgg ggtcagcccg
     2701 agcttccgcc gaccacggcg agcacctccg ggccgtagcg ctcgatcttc gccgggccga
     2761 ccccggagat cgatcggagg tcggggaggc tccgcggacg gcgtgccgcg atgtcggcga
     2821 gggtcgcatc gtggaagacg acgtaggcag ggacgccatc gcgcgccgcg cgctccttcc
     2881 gccatcggcg cagcgcctcg aacagcgggt catcttcttt cgccgcggcc ggctgctccg
     2941 ctccccggac cggggccccc tcggcgccga gctcgtcgag gaacgggctg cgggaggacc
     3001 gcgcgtcggt cggccacgtc aggtgcagga tccgcttggc gcgcgtgatc ccgacgtaga
     3061 gcaggcgtcg ctcctcgtcg accggcgccc ggtgccgccc cgagcggaac ggcagctcgc
     3121 cgtcgaggag ccgcggcagg agcacggcct cgaactcgag gcccttggcc cggtgatacg
     3181 tcatgaggtt caccccgcga ccctcacgct ccgtcgagaa gcgccgggag agctcatcga
     3241 ggaaggccgc gacgtcgtcc tcgggccgcg cttcggcgaa ctcgcgcgcc aacgtgcgca
     3301 accgtgccag gtcggcccgc cgggtgacct cctcctccga gtccgcctcg ccgggagcat
     3361 cgaggccgag cgcggccgtc acgccgtcga ccgcctccac cacctcgccg ccggttcgac
     3421 gcaagcgcgc cagcgccgag cgcggacccg gccgccgcag gaacgcgcca tcgcgcacct
     3481 ggaacgcgat cccctgcgcc gccagcgcct cctcgaacgg ctcggatcgc gcgttgatgc
     3541 gatagagcac cgcgatctcc tcgagtggga tgccttccgt gcggagtcgt cggaccgtct
     3601 ccacgacgtg ggcggtttcg gcggcctcgc cggcgagcgg gcggatcagc ggcggcggac
     3661 ccgcgggacg agcggtgcga agcgtcttcc ggtatccgcc cagccgagac cccagccggt
     3721 tcgccaccgc aaggacctgg ggggtcgagc ggtagctctc ctcgagcgtc acgaccttcg
     3781 cccccgggaa ccgatcgggg aaccgcagga ggtacgacgg cgtcgccccg gtgaagccgt
     3841 agatcgtctg gtggtcgtcc ccgacgacgc agagatcgtc ccggtcgccg agccatcggt
     3901 cgaggagcgc ttgctggagg ggattcacgt cctggtactc gtcgaccgtg aaggctcggt
     3961 agcgcccgcg gacggcatcc gccgcttcgg cgtggtcgtc gaagagctgg agggcacggg
     4021 cgagcatgtc ctcgaagtcg aaccggcccg tcgcacgctt ccgccgttcg tacccggcgt
     4081 gcacccgctc catcaggtcc ggcgggatcg gaggctgatg tccggtccgc cgaagctcct
     4141 cggcgtagcg ctcgggcgcg accagtcggt tcttcgccca ctcgatctcg gtggcgagct
     4201 cacgacgggg aacgaagcgg tgcggcgggg gcagggcgtt cgccatcgag gcgatcagcg
     4261 gggccttcgc atcgaggacc tcccccagcg gcgtgccggt cgacggctcc cacagacggg
     4321 agagctggga gagcgccgcg gcatggaagg tccgagcctc gaccccttcc acgccgagcc
     4381 gggtcagccg cgccttcagt tcgccggcgg ccttctcggt gaaggtcacc gcgaggatct
     4441 cggagggccg gaacgagccc gtggcgacct ggtaggcgat ccggcgcgtg atcgtggtcg
     4501 tcttcccggt cccggcgccg gcgatgatcg cgacgggccc cctcgtcgcc gttacggcct
     4561 cgcgctgggc ggggttcagc ccgtcgagga tgggggcggc ggcggccgga gtcacacggg
     4621 gatgctaccg gccgccgccg acgtctacgg gttccgcatc cgccaggact ggatgtcgtg
     4681 gacggccgcg gcaccgcccg tggccgccgt gaacccggcg tgcccggtgc cgccgagcac
     4741 ctcggcgagg tcgaccttcg tcacgagcct cggccggtcg ggcttcgtgt cgctctgcgc
     4801 gacgaacacc ttcagccgct cggtgttcgg cgagtacgcg atccacaccc gccagtcccg
     4861 gtcgtacatg ttcatgccgg cgctcgcgag gggcgcgcgg tcggtccgtc cgttcgtacg
     4921 gacccggacc cggccctggg agcggccgta gtggaggttg aactccaccg cgacgctgcg
     4981 tcggatccga acctgctcgt cttcccgctc gccgtacccg agatgatggc cgttcgaacc
     5041 gagggcctcc gctccgcggg ggtcgcggtg gatcacgaac gcgaggccgt cggccgggtc
     5101 acccgagtcg tggatccgga agcggaactt cgttcggatc gacttcgagg ggttcacgac
     5161 cttcgcctcg ctgaacgccg agccggcctg gtcgccgctg ttctgggtca gccgcaggac
     5221 gcctccctcc gcctgcgcgg agccgttgag ccgcagcccg ccgacgttcg agaaatccgg
     5281 gaacgcgacc gacccggccg aggcgggtgc cgccgccaaa cccaggatgg cccccatcac
     5341 ggtcaccacg acggtccggc cgcctcgcct gcccgtcatc aagctcgtct tcatcgctgc
     5401 ctcctccggt cgggacgctc ctcatggcat cggagccgcg gggggccggg ccgatagccg
     5461 cttcgagcca ttccgcgata gccacggatg actagggcgt gtggccctgg aggtcctcga
     5521 aggcgggctg gatgggaacg acgtcgtaga cgggccagcc gtcctcgaca cggacgatgt
     5581 ctcgaagcgc gtgcgcgcgg atcgaaccgt ccggcatcag gtgtcgcgcc gcgtgccctt
     5641 gcgccacgac agcatcggcg atgagcccgc ggtggcatcg ccaccacacg gcctcggcgc
     5701 acatcacggt cgtcggctcc tccgccgcgc gctccaggac gcgaccgagg gccgaccgga
     5761 acgcgaccgt tcccatgtgc tccgcgtagc cgcggaagga gtcgttgcgc aacgccgtat
     5821 gccgtgaccc gggcggcgcc ttgcggaagc ctccgagggc ctcaccctcc caggtgtagc
     5881 cgagccccgc cgaagcgagc gcccgctcga gggcgtctcg ggcgaagtga gggtgccgcc
     5941 gcgacgcggg gacgacccgg acgtcgacca cgtgccggag accggcctgt cgcacgaggt
     6001 cgatgaaggc gtcgatcccg tgggtgctgt gcccgaccgt gtacagcgcc gtcatccgag
     6061 gcgcggggtc acgcgtcggc cacgacggcg aacagcgggg tcgactccag cctcgagatg
     6121 agggtcggtg cgatcatgcg gcgggcccac tcgacgtcgc gcagacgctc cactcgcctc
     6181 cccttccagc cggcgcggcg ggccgctccg aggaggggtg ccggcgttcg cgcgtggagg
     6241 agcgggagcg cacgcaccac gtcctctccg tagtggtcgt ggtggtcgtg cgggacgtcg
     6301 aggacccgcc ggacgagaga cgcgaaagcg aagcggaccc ggcgctcgat ggtggaagga
     6361 ccgagtccct ccatcgccac gagccgaccc tcgggggcca cctcgcgcca cgccgctagg
     6421 gtgcggacgg ggtcggggtt cgtccacagg acgtggcgct ccaccaccgc atcgaacggt
     6481 cccggcggcg gctcgtcggc cggggcgacg acgaactcga catcgcgacc cgcgagcttc
     6541 gtccgcgcgc gatcgagcat cgccgaggag atatcgagcg ccgtcgcgcg gtagccgaga
     6601 tcgaggagga gcgccgtgat cgcgcccgtc cccgccccgg cgtccaggac gtgcgcgggt
     6661 ggcggcggca ggtgccggcg gaggagcgcc ctccacgccg ccgcctccac ggggtccgtc
     6721 agcgcgtgcg aggggctctg gtcgtaggtc tctgcgtcgt ggtcccagaa ctcgcggatc
     6781 tggtctcgga tgtcggccat gtccccaggc tacccgcctc gccgatcgtt gcgctcaaga
     6841 accgtggcgc tgcgggcgat acccccggtt gaggaggagc catgatccca ccaccgatcc
     6901 cgtcggacga acaggaccga ctggccacgc tgcacgccct ccagatcctc gacaccgagc
     6961 cggaagagcg cttcgaccgg gtgacccgat tggcccagcg gctcttcgac gtcccgatcg
     7021 ccctggtgtc gctgatcgat gcagagcgac agtggttcaa gtcgaagatc gggctcgagg
     7081 cctcggagac ctcacgcgag gtgtccttct gcgcgcacgc gatcgccggc gaagggatcc
     7141 tgcacgtgcc cgacgcgcgc gaggacccgc gcttccatga caaccccgat gtcctcggcg
     7201 gagcgaacat ccggttctac gccgggtgcc cgatccaggc acccgacggc aggtccatgg
     7261 gaaccctctg catcgtcgac gaccagcctc ggcacgtcga cgaggaggcg ctgagctcgc
     7321 tgcgggacct cgcggagatg gtcgagcagg agctcgccgc cctggcgctc gccgtgcagg
     7381 acgagctcac cggtctcgcc aaccgccgcg ggttcgtcgg gctcgcggcg caggtgctcg
     7441 cggtggcgat gcgccaggga ctcccggcga cgctcgcgta catcgacctc gacggcttca
     7501 aggcgatcaa cgacacgcac ggccatgcgg tcggtgaccg cgcgctccag gacgtggcgc
     7561 ggatcctgac cgagtcgtgt cggtcgtcgg acgtgctcgg ccgcttgggg ggcgacgagt
     7621 tcgcgctcct cttcctcgca gaggagtcgg cggccccgct ggaccgcctc cacgacgcgg
     7681 tcgcgcgaca caacgacctc gcgggccgcc cctaccgcct gcagctgagc gtcggaaccg
     7741 ctcggttcga cccggaggag ggcgtcggcc tcgaagagct catggaacgc gccgatcgct
     7801 cgatgtacga gcgcaaggct cgccggcagc gggcctctgc ctagctgccg ctaaccgacg
     7861 gagccttcca tgttgagctc gatcaggcgg ttgagctcca cggcgtactc catcgggagc
     7921 tccttcgtga tcggctcgat gaagccgttg acgatcatcg ccatcgcgtc ggcctcggac
     7981 atgccccggc tcatcaggta gaagagctgg tcgtcgccga ccttcgagac ggtcgcctcg
     8041 tggccgatcg cggcggtctc ctcctcgatc tcgatgtagg ggtaggtgtc cgagcgcgac
     8101 tcctcgtcga ggatcagcgc gtcgcagcgg acgctcgact tcgcgtgatg cgcctcgggc
     8161 tcgacgcgga tcaggccgcg gtaccccgtc cggccgccgc ccttcgagac cgatttcgag
     8221 ttgatgatcg aggtggtgtg cggggcgacg tggatcgcct tgccgccggc gtcggtgtgc
     8281 atccccggcc cggcgtaggc gaccgagagc acctcgccgc gagcgccctt gcccatcagg
     8341 tagatgctcg ggtacttcat cgtcaccttc gagccgatgt tcccgtcgat ccactcgacg
     8401 accgcgtcct cgtgggcgac ggcgcgcttc gtgaccaggt tgtagacgtt ggtcgaccag
     8461 ttctggatcg tcgtgtagcg cacgcgcgcg gtgggcttgg cgatgatctc gacgacggcc
     8521 gagtgcaggg agtcggtcgt gtagatcggg gcggagcagc cctcgacgta gtgcacgtag
     8581 gacccctcgt cggcgatgat cagcgtccgc tcgaactgac ccatgttctc ggcgttgatc
     8641 cggaagtagg cctggagcgg gatgtcgacg tggacgcccg gaggcacgta gatgaacgag
     8701 ccgcccgacc agaccgccga gttcagcgcg gcgaacttgt tgtcgttcgg ggggatgacg
     8761 gtgccgaagt actccttgac gaggtccggg tggtcgcgca gcgcggagtc catgtcggtg
     8821 aagatcacgc cgagcttgtc gagctcgtcc ttgatcttgt ggtacacgac ctcgctctcg
     8881 tactgcgcgc tcacgccgcc gaggaacttc ttctcggcct cagggatgcc gagcttgtcc
     8941 caggtggact tgatgtcatc ggggaggtcc tcccactcct tcgcctgctt ctcggtggag
     9001 cggatgaagt agtagatgtt gttgaagtcg atctcggaga ggtccgcgcc ccaccagggc
     9061 atcggccggc ggtcgaagtg ccgcagggac ttcagccgga acttccgcat ccactccggc
     9121 tcggacttca tgaaggagat ctcctccacg acccgctcgg agaggccccg cttgggctcg
     9181 aacacgtggt gcgcggggtc cgaccacccg aagcggtagc cctgcgcgag atccttgagc
     9241 tgctcgtcct gcgtcttcgc catggctcca gtgtatccgg aaatctcgac gtgtgcggtc
     9301 ggatttgacg ctctagccgg gcgggaccgc cggccggacc caggggctga accgccggtg
     9361 catgaacggc tcccggtagt gcggctccca ggggtcgtac agccgttcgt gctcgggatg
     9421 gtgctcccag gcccggacct gctcgaccgt gtcccactcg ctgaccacga cccatacgcg
     9481 cgggtcccgc gggtcctgcc cgacctccgc ccagcggtac cccggttgct ccgcggcgag
     9541 gatcctcatc gcctcgatgt ccccggcgaa cgcctcgagg tcggcatcct ccgtcagggc
     9601 gaagcgcagg taggagagga tgctcacgcg ctcagaacat cccgagggcg gccttcgcct
     9661 cctcggacat cttctccggc gtccacggcg ggctgaagac catctgcgcc tcgaagtcct
     9721 tgaccccgtc gatgtcgtcg acgagcccgc gcatctgttc ctcgatcatg gggccgatcg
     9781 ggcagcccat cgtggtcagg gtgtaggtga ccttcacggt cccttcctcg atcgtgaggt
     9841 cgtacacgag gccgaggtcg acgatgttga tcccgatctc ggggtcgttg accttcgcga
     9901 gagcctggac cacccgcttg cgcgtctcct cgtcccctcc ctcgccggga agggttgtca
     9961 cgtctgatac gaaatctccg ttcggttctt ccatcgtccg cctccggaga ctatcctact
    10021 cgtgaccgga ccctctcctg cgggaggcac tcacatgcgg gttctgatcg tggacgacca
    10081 ccccgtcacg cgcgacggtc ttcgcgccgc gctcgggacc gccgaggacg tcgatatcgt
    10141 gggcgaggcc tcgtcgggcg acgacgcggt gaaggccgtg gatgatctgc ggcccgaggt
    10201 cgtcttcatg gacgttcgca tgccggggat gaacggcatc gaagccacgc ggctgatccg
    10261 cgaggagcat cccgagacga aggtcatcct gttcacggtg gacgagtccc gtgcctccat
    10321 ctcggaggcc atccaggctg gggtctcggg ctacctcctg aaggacgtcg gcgtcgatga
    10381 gctggtgaac gccgcccggc tcgcgctcga ggggaaggcc gtgatccacc ccaccctcac
    10441 gcaatccttc atcgaggagg tccgcctcgt cgagcggaag ccggaggcgc cgctctcccg
    10501 ccgagagatc gagatcctcc agaaggtcgc ctacggggcg acgaccaagg aggccgcgga
    10561 ccaactgggc atctcgcccc acaccgtcaa gacccacctc gagcgcatct tcgagaagct
    10621 cggcgcgaac gaccgcgccc aggcggtcgc gatcgcgatc cggcgcggga tcgtcgagta
    10681 acggtcgccg ggtaaggctg ggcaccgcaa cgtccgatac tccaggcatg tcgtccgtcg
    10741 tggtctgccg tgctcctggg accctcgccg acgccgaccg ggcgttcgcg ggagagacgc
    10801 tcgaacccgt ggacgacggc taccgggccg tcgaggtggc gcagcggacg cgcgccgatc
    10861 tcgtcgtggt cgacctcggc gtcgcggcgc tctcacccgg cgagctgatc aagcggctgc
    10921 gttcgtcgaa cgaagaggtg cgggtcatct tcgtcctcgc gcgcccggcg gtcgaagagg
    10981 ccgccgacgt cctgcgggcc ggggcggatg gggtcttggc accgcgaccc tcggccgacg
    11041 cgctgcgctg ggcggccgac gaagcccggg cgggcggttg cccgatctcg ccgtccctcg
    11101 cgcggggact cgccgagcgg ttcgccgaca tcgtgcatcg cgagcagcag tggtcgaaga
    11161 cgctggcgga tgtctcccgc caggccgagg agctcgccca cacgaaggcg gagttcctct
    11221 ccaacgtctc gcacgagctg cggacgccgc tgacgatcat caagggcgtc gcgcaggtcg
    11281 tcggccggtt cggcaacgcc gacgagcagc aagccggcat gctggcccag ctcgaggagg
    11341 cggcgacgaa gctcacccgc atggtcgaga acctcctggt gatcgcggag atggagcgcg
    11401 gggacttccg cctcgacctc gccgaccacg acctcgcgga gctcgtgcgc acgagcgcgg
    11461 aggccgcggc ggtccggtac ccggccgtca cgctcgacct ccgcatcccg ccgttcctgc
    11521 gcgcgacgct tgacgccgaa cggatgggtg aggtgatccg gcacctggtc gacaacgcct
    11581 gccgctactc cgacgagggc gacaccgtca cggtccaggc ccggaaggcg agcgagggga
    11641 tcgtcatcac cgtcacggat cagggacatg gcgtcgaccg caaggtggtc tctgccgcct
    11701 tcggcgaggc gttctcccca ggcgagtccg tcctgacgaa ggagaaggcc ggcctcggcc
    11761 tcgggctcaa cctcgcccga agcgtggtgg cccagcacgg cggcatcctc tcggcggacc
    11821 ccctccccgg gggcgggtcc cggatctcgg tgatcctccc accggaagtg aagccgcagg
    11881 ccgcgccggc cgcagccacg aacggcgacc acgatcagca ggacgaagca ccggaggatc
    11941 cgatggcgct cctgcgggag ctccagcgca agctctcgga ctcggagagc cgcgtccagt
    12001 aggccgcacc gcgcaggcga cccctcccct acactcggac catgcggcgg agcggcatca
    12061 tcctctcctt catcgtcctc gccttcgccg cgggaccgac ggggccctcg ctcgataccc
    12121 cgcccgccga agggtccgag gttcggctcg tgcggatcga ccgcgtcagc gccgtcctcc
    12181 cgaccgactg gggcgtggaa ccgctcgccg acgagggccc ccggcggggt ctccgcgcgt
    12241 cgagcgacct cgaggcatgg acgacgggtg cgagccgcgg actcgaggcc tactgggtcg
    12301 acgcgaccgg cgtcggggtc ccgagcgact actactacct cgtcgcgagc ggtccggggg
    12361 cgacgcggat gccgcacccg cgcaactgcc gcaccgacga cgagcgcgtc ctgctcgacg
    12421 cgcgcccgcg accgcaggcc gacgtcgccc ggagcggatc gttcgtcgcg atctcgaccg
    12481 gcaggtgcgg ggggtccgcg gatcgcaccc ggtgggtctc cttcgtggcc gcgcccggct
    12541 tcggtccggt cggcgagatc gggatccgcg agtcgggcct ctactacggc ctcgcggtcg
    12601 tcgaagacgg ggcggacgcg gctcgggagg ccaagcggct cctggcggag gttcggttcg
    12661 acaccacgag tgtgcgccgc atcatcgagg cggcgggggc gagcctctag gtcaggtctt
    12721 cgggctgctt gcgacgaagg gcggcttcac gatctcggcc tcgccgcggc ggccgcggac
    12781 ctccacctcg acggtcgagc ccacggagaa gcgctcccgc ggggagaggt acgccatcgc
    12841 gatccccacg cggagggtcg gcgagaacgt cccgctcgtc gtctccccca cctgctcccc
    12901 gtcggcgaac accgggtagt gcgcgcgcgg gatgaggcga tcccgcatcc gcagggccca
    12961 caggcgggat gggatgcctt cctccttctg ccgcaggagc gcctcgcgcc cccggaaggg
    13021 gcccttgtcg agcgccacgg accacgacag cgcggcctcg aggggcgtgc gctcctccga
    13081 gatgtcgttg ccgtggaggg ggtagcccat ctcgagccgg agcgtgtccc gcgcgccgag
    13141 gccgcagggg acgatcccgt gggtctcgcc ccgcgcgagc acctcccgcc agagcgccgg
    13201 cgcgatgcct tccggcgcga acagctccac ccccggctcc cccgtgtagc cggatcgcgc
    13261 cagcagcacg aggacgtcgc ggtaggagac ctcacggcac tccatgtagg cgagctcccg
    13321 cacctccggg aacatgccgg cgacgagctc gagcgcgcgc ggcccctgga cggcgatcgt
    13381 cgtcaggtcc ggccggtgcg tgacgacgac gtcctgccgc tcctcctcct gcagcaccgc
    13441 gagcaccttc gccgcgttcg ccgcgttcgg gacgacgagc cagcggtcct cggtcacgcg
    13501 gtacgcgatc aggtcgtcga tgatcccgcc gctctcgttg agcacgaggt tgtactgggc
    13561 ctggccgacg cccaccttcg acaggtcgtt cgtgaggacc gtctggagcc acccgagcgc
    13621 ccccgcaccc gcgacgtcga ccttcccgag gtgggtcaga tcgaagatcc cgaccgcctc
    13681 tcggaccgcc cggtgctcgg cgagggtccc ctggtattcg atcggcatcg cccagcccgc
    13741 gaacgcgccc atcttcgcgc cgagcccccg gtgctcctct tcgagcgggg ttcggacgag
    13801 atccatcgac acctcctgcg cgcgatagag tatggcgtgg cgccggaccg gcccggcgcc
    13861 gcccaagcgt atccgaccga ggggagagac cgagcatgcc gcaggatccc tacgacgtca
    13921 tcgtcatcgg cgctgggacg ggcggatact cctgcgcgct gcgggcggcg cagctcgggc
    13981 tctcgaccgc gctcgtcgag cgggacgagc ggctcggcgg gacctgcctg ctgcgcgggt
    14041 gcatccccac gaaggcgctg ctgcagtcgg catcggtcct cgaccacgtg aaccgctccg
    14101 acgagtgggg catcaaggcc tcgggcgagc ccgactggag cgcgatccag gggttcaagt
    14161 cgaagatcgt cgacaagctc gtctcgggcc tcaccgggct cgtgaagatg cggaagatcg
    14221 aggtcatccg cggcacggcc acgatcaccg cgccggggac ggtggaggtc gagggcgccg
    14281 gaacgctcca ggcgcggaac atcgtggtcg cgaccggctc ctacccgaag ctcctgcccg
    14341 gcctcgaggt cggcgagcgc atcatcacga gcgatcaggc cctcgagctc gaccggatcc
    14401 ccgcgagcgc ggtcgtgatc ggcgccgggg ccgtcggtct cgagttcgca tccctctacc
    14461 gctcgttcgg ggccgacgtc acggtgatcg aggcgctccc ggccatcgcg ccgctcgagg
    14521 accccgacat ctcgaaggag atggcgcgag ccttccgcaa gcgcgggatc gcgacccacg
    14581 ccggcgcgaa ggtgagcgag gtgaaggaga ccggcgacgg cgtctccgtc gccttcgagt
    14641 cgggcggcaa gggtgagacg gtctccgccg agctgtgtct cgtcgcggtc ggccgcgggc
    14701 cgatctccca gggggtcggg ctcgagaacg tcggcgtcaa cgtgaacgag cgtgggtacg
    14761 tcgaggtgga cgacatgctg cggacgagcg tcgaggggat ctgggcggtg ggcgatgtcg
    14821 cctccacgcc cttccagctc gcgcacgtct ccttcgccga gggcatcgcg gtggccgagc
    14881 gggtcgccgg caacgacgtc cccgccatcg actacgccgg cgtgccgcgc gtcacctaca
    14941 gcgcccccga gatcgcctcg gtcggcctca ccgagcagca ggcgaaggat cgcggccacg
    15001 acgtcgtgac cgagaagctc agcatgcagg gcatcggcaa ggcgaacatg ctcggcgagg
    15061 gcggaaccgt gaagctcgtc gccgagaagg acgggccggt gctcggggtc cacatgctcg
    15121 ggccgcacgt gaccgacctg atctccgagg gcatgctgat cacgaactgg gaggcgctgc
    15181 cgaccgaggt ggccgccctc atccaccccc acc
//
LOCUS       DATLGG010000316        10789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_79087, whole genome shotgun sequence.
ACCESSION   DATLGG010000316 DATLGG010000000
VERSION     DATLGG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79087"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VM638_08950"
     CDS             <1..167
                     /locus_tag="VM638_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HVM12584.1"
                     /translation="SIPGVTSAEDVTGPYDVIVRAEAANVDDLGKLVVARIQGVEGIT
                     RTLTCPVVHL"
     gene            complement(188..1267)
                     /locus_tag="VM638_08955"
     CDS             complement(188..1267)
                     /locus_tag="VM638_08955"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HVM12585.1"
                     /translation="MTAARIRVAIVFGGRSAEHEVSVVSARSVMDALDPERYEPVPIG
                     IDQAGRWYLLPEGPPALQPGSGSLPRVEAAPAAAVTLARDPGRREIVTGTGERRPIDV
                     VFPILHGPYGEDGTIQGLLELAGVPYVGSGVLASAVGMDKAVQKALFVAVGLPVVAHE
                     VVTARGWREDPEQVHATVEDLGYPVFTKPAGLGSSVGITKVKSAADLDAALDEALRHG
                     RKALVERAMEGFREIECGVLGNDDPVASVPGEIVPGAEFYDYRAKYLDAGTRLEVPAL
                     LPGEVVERVQQMSIAAFSAIDAAGMARVDFFVRDQEILVNEINTIPGFTEVSMYPRMW
                     EASGLPYRDLVDRLIGLALERHGTA"
     gene            complement(1264..2439)
                     /locus_tag="VM638_08960"
     CDS             complement(1264..2439)
                     /locus_tag="VM638_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017597122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVM12586.1"
                     /translation="MEERGFTTRSLRAAADARGAEPLSVPIYQTATFAFDDAEVLAEW
                     IRQGKDAGHVYTRWNNPTREALERALADLERGDGAVSFSSGMAAITTTLAALVQSGDH
                     VVSSPDLYGGAFSTMTKLLPRWGVDVTLARSHRSEDMLAEIRPGTVLCYTESIGNPRV
                     SVPDLSALADGCRERGVTTVVDNTFASPYLCTPRAFGIDVVVHSTTKYIGGHSDLTGG
                     IAVTDVETHHRIRETSIDLGGVSSPFEAWLALRGLQTLALRMERHCANAQMLAEMLER
                     HEKVTEVWYPGLPSHPDHDLATRQMRGASGMLAFELAGGLEAGRRFMEAVEVARVGAS
                     LGSTHTLVVHPPTVTHTQMTAEERHASGISDGIVRVSVGIEDPEDVLEDFERALEKA"
     gene            complement(2477..3466)
                     /locus_tag="VM638_08965"
     CDS             complement(2477..3466)
                     /locus_tag="VM638_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HVM12587.1"
                     /translation="MTEDQRAPEPSLGDILAARRLIDAHLPRTPFRESIALGRQLGLR
                     LWIKYENHQPVGAFKVRGNINKVLSLDPEERLRGVVTASMGNHGQGLCYAARLVDVHP
                     IVVMPESANPDKVAAMRDLGAEIVFEGRDFDEADAKARELEAEKGLVYFHAADDPWIV
                     AGHGTIGLEMLEAVPDLDALVVPVGAGGLISGVALAAKAIRPEVEVIGVQAKACAPYL
                     ASRREGRRVEAPSAETFAEGLAARVPTSLPFAMIDRHVDDLVDATEEELRQAILLLLD
                     ATHNLAEGAGAAGTAGVVKLADRLAGKRVGTILSGGNLPRAVLERALCDPAPW"
     gene            complement(3463..4137)
                     /locus_tag="VM638_08970"
     CDS             complement(3463..4137)
                     /locus_tag="VM638_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVM12588.1"
                     /translation="MKRPEPWMRLAESVCIPPLWTWFKWRFEGLEHLPRQGPALVACN
                     HISYLDPLCHGYFVEKGKRRPRFMAKIELYRNPLLRAVLRGARQIPVRRGSGERAPVE
                     AALKALAAGEVVMVYPESTVTKNDDFSPMAGKTGIARLTLATGVPVIPLAVWGSQHVW
                     QREGTRSLRFGRPIWVKAGRPLSFSLQDEDPDHPQVLRRVTDEVMSELSVLVDDLRAR
                     YPKEWS"
     gene            4217..4858
                     /gene="cofC"
                     /locus_tag="VM638_08975"
     CDS             4217..4858
                     /gene="cofC"
                     /locus_tag="VM638_08975"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HVM12589.1"
                     /translation="MRICAVPVKALERAKGRLADTLAPLERAALTLAMLEDVLDACLR
                     LPGWHVVVISPDQAVLEVAAKRGVRPLEEEEPGLVAAVRQVEEAFPTAEQLLVVLGDL
                     PLLTGESLQRVVRTLGPVVAAPSSSDAGTNVLLRRPPGIVPPLYGTASFERHREAAEL
                     RGVPFSVVRAPELQFDLDRGEDLAQLLTWPRPTRTKAACLEMGLPDRLNLRVP"
     gene            4870..5079
                     /locus_tag="VM638_08980"
     CDS             4870..5079
                     /locus_tag="VM638_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HVM12590.1"
                     /translation="MPQGTIKEFDKGSRTGILYLDDESEVQIDASSVEGADIQHLRIG
                     QRVKFDLAEEGGRRLARVLRIVTFE"
     gene            complement(5080..5397)
                     /locus_tag="VM638_08985"
     CDS             complement(5080..5397)
                     /locus_tag="VM638_08985"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVM12591.1"
                     /translation="MNKQDLVNEVARRTGLEPPEAADAVEAMISAIRESVAKGQRVTL
                     SGFGTFEKQRRAARLGRNPHTGEPVPIAATTVPSFRPGKLFREAVLPKRRKRTTRTAA
                     RRR"
     gene            complement(5407..6189)
                     /locus_tag="VM638_08990"
     CDS             complement(5407..6189)
                     /locus_tag="VM638_08990"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HVM12592.1"
                     /translation="MPPTSSEPAAAIDWIAPDPPVRIERPLLSQAWRHCAFLHWPYDP
                     EAIRPHVPSAFELDLHDGRAWVGLVSFRIAHMNPGRRLPPIPGLRTAHESHLRTYVRG
                     PDGRRGIWMFSLDMAPLQAAALGRAMWFPYRWATNEVRAGADRARYRTRRRSSGGGGL
                     RLDLDLGAAIPADELTRFDHFLTARWVLYTGLGPISAGLLVEHPRWVFRRVTARRIEQ
                     TLTDRAGFPRPEVAPLVHYSDGVDALLSWPRLSGRGGRSATT"
     gene            6310..6383
                     /locus_tag="VM638_08995"
     tRNA            6310..6383
                     /locus_tag="VM638_08995"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6342..6344,aa:Gln,seq:ctg)
     gene            6384..6456
                     /locus_tag="VM638_09000"
     tRNA            6384..6456
                     /locus_tag="VM638_09000"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6418..6420,aa:Glu,seq:ctc)
     gene            6784..7122
                     /locus_tag="VM638_09005"
     CDS             6784..7122
                     /locus_tag="VM638_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVM12593.1"
                     /translation="MARLINASRAERGRRTLRISERLSSVAQKHASAMASAGRLYHNP
                     SLAQQLSTFNWTILGENVGVGATVPTLHRAFMASDAHRRNILRRPFRRVGVGTVMAGG
                     RLWVVVVFSD"
     gene            7402..7551
                     /locus_tag="VM638_09010"
     CDS             7402..7551
                     /locus_tag="VM638_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12594.1"
                     /translation="MSLLVILIILALVAGGLGLLVEGLKWLLIIAAILFIAGIFTGFR
                     GRSRV"
     gene            complement(7548..8456)
                     /locus_tag="VM638_09015"
     CDS             complement(7548..8456)
                     /locus_tag="VM638_09015"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVM12595.1"
                     /translation="MRLRGTWFVVPTPFREDGSLDEESQRRLLDAAITWGVDGLTVLG
                     VTSEAAELTEEERDRVLRTVAEEVGDRVPVVVGCSAPDPDRIVARATAAADAGAAAAM
                     VSAPPGFSDPEGLPGIYGAVLDRGGLPIVVQDEPAATGVRMDLEILLRCLDAAGSATV
                     KLEDPPTPPKIGRLLSARPGLDVFGGLGGVMALSELRRGACGTMTGFAFPEILSAVRR
                     GTEAGEHEAAARIFDRYLPLIQFEAQPLIGLGIRKEILRRRGVIATAVTRRPADVEAG
                     TLADLDDVLRRAGIVPGPEPLSAEPG"
     gene            complement(8453..9640)
                     /locus_tag="VM638_09020"
     CDS             complement(8453..9640)
                     /locus_tag="VM638_09020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVM12596.1"
                     /translation="MPVRYVSDPPSTADVVVIGGGIVGAATAFHASAAGLRTVLVERR
                     PALCTLTTAAAAGGFRLQLDTEEEFLVVRGSVELFHAFAERTGQAVYDPKVREQGYLW
                     VTTSEDAVEGQRRLAETQASWGVDGIEVLDREEVVRRFPFITPEVVQARYRAGDGLVD
                     PKAVTFGLAAASGADVVVRCGVTGFRIEGDAVVGVETERGTISTRNAVIACGPLSGRL
                     AERVGIDLPIHTVRRHKLVLPEVPEVPQDAPMTIDEDTGAHWRPAFRGAFALFTDPTT
                     PASPPEEVVTPDPSFAFSVLDPRSPAALARIAPFWHSVWERGSDHWVLQAGQYTMTPD
                     RRPLIGQTALPGLWVNTGYSGHGVMMSPAASSLVVDGIVGRDRTNAFRPDREFAVPHT
                     GVL"
     gene            complement(9680..10213)
                     /locus_tag="VM638_09025"
     CDS             complement(9680..10213)
                     /locus_tag="VM638_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVM12597.1"
                     /translation="MAHFAVTAIGADRPGIVAALTEALYGIGGNLEDVASTILRGHFA
                     MMLVVNAPRDVDATALEKALGQAAWPLRVTVTVRSLDPGRRSNPSATHYLVVYGSDQP
                     GIVARLSRLLTERGANINDLSCRLVGSADEPVYAMIAEVAVPEAADAGALEREIEDIA
                     NDLAVDVTLRPIEVETT"
     gene            complement(10216..>10789)
                     /locus_tag="VM638_09030"
     CDS             complement(10216..>10789)
                     /locus_tag="VM638_09030"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVM12598.1"
                     /translation="SFDEKTTFFTREELIERFDLARVSHNPAAFDREKLEWMNGHYIR
                     EAADDRLGDLIEERLASGGTAVDRSLLDPAVPLVKERMKLLSEAPGLLRFLFEEVEPD
                     EKAAKMLAGQTEYLSEVATRLEKVEPWSAAGIEGVLRELQEERELGARKAFQPVRAAV
                     TGTLVSPPLFESIELLGRDRTLARLRSLRD"
BASE COUNT         1551 a         3890 c         3815 g         1533 t
ORIGIN      
        1 ccagcatacc gggggttacc tcggccgagg acgtgaccgg accctacgac gtcatcgtgc
       61 gcgcggaggc cgcgaacgtg gatgacctcg gcaagctcgt ggtcgcgcgg atccaggggg
      121 tcgaggggat cacccggacc ctcacctgcc cggtcgtcca cctctaggcc gcactcgacg
      181 cccgccctca cgcggtcccg tgccgctcga gggcgaggcc gatcagccgg tcgaccaggt
      241 cgcggtaggg aagacccgac gcctcccaca tccgcggata catcgagacc tcggtgaacc
      301 ccgggatcgt gttgatctcg ttcacgagga tctcctggtc gcggacgaag aagtccacgc
      361 gtgccatgcc ggcggcgtcg atcgccgaga aggcggcgat cgacatctgc tgaacccgtt
      421 cgacgacctc gccggggagc agggccggaa cctcgagacg cgtcccggcg tcgaggtact
      481 tcgcgcggta gtcgtagaac tccgcgcccg gaacgatctc gcccggcacg gaggcgaccg
      541 ggtcgtcgtt gccgaggacg ccgcattcga tctcgcggaa cccctccatc gcccgctcga
      601 ccagcgcctt gcggccgtgc cgcagggctt cgtcgagggc ggcgtcgagg tccgcagccg
      661 acttcacctt cgtgatcccg acggatgagc caaggcccgc cggcttcgtg aagaccgggt
      721 agccgaggtc ctcgaccgtc gcatggacct gctcggggtc ctcccgccag ccgcgtgcgg
      781 tcacgacctc gtgcgcgacg accgggagcc cgacggcgac gaagagcgcc ttctgaacgg
      841 ccttgtccat gccgacggcc gacgcgagga cgccggagcc gacgtaggga accccggcga
      901 gctcgaggag accctggatc gtgccgtcct ccccgtacgg gccgtggagg atcgggaaga
      961 cgacgtcgat cggccggcgc tccccggtgc ccgtgacgat ctcccgcctt cccggatcac
     1021 gcgcgagcgt gacggcggcg gccggcgcgg cctcgacccg cgggagcgag cccgaccccg
     1081 gctggagcgc cggcggcccc tcgggcagga ggtaccagcg ccccgcttgg tcgatcccga
     1141 tcgggaccgg ctcgtagcgc tcgggatcga gcgcatccat gaccgaacgc gcggacacca
     1201 cggacacctc atgttccgcc gaccggcctc cgaagacgat cgcgacgcgg atccgcgccg
     1261 cggtcacgcc ttctccaggg cccgctcgaa gtcctcgagg acgtcctccg gatcctcgat
     1321 gccgaccgac acgcgcacga tgccgtcgga gatcccgctc gcgtgccgct cttcggcggt
     1381 catctgggta tgcgtcacgg tcggcgggtg gaccaccagc gtgtgggtgc tcccgaggct
     1441 cgccccgacc cgcgccacct cgaccgcctc catgaagcgc cggcccgcct cgaggccgcc
     1501 ggccagctcg aacgcgagca tgcccgaggc gccgcgcatc tggcgggtcg cgaggtcgtg
     1561 gtcgggatgc gaaggcagtc ccgggtacca gacctcggtg accttctcgt ggcgctcgag
     1621 catctctgcg agcatctgcg cgttcgcaca atgccgctcc atccgcagtg cgagcgtctg
     1681 gaggccgcgc agcgcgagcc aggcctcgaa cggggacgac acgcctccga gatcgatgga
     1741 cgtctcgcgg atccggtggt gggtctcgac atcggtgacg gcgatgccgc cggtgaggtc
     1801 ggagtggccg ccgatgtact tcgtcgtgga gtgcacgacc acgtcgatgc cgaaggcgcg
     1861 cggggtgcag aggtaggggc tcgcgaaggt gttgtccacg accgtggtca cgccgcgttc
     1921 gcggcagcca tcggccagcg ccgacaggtc cgggaccgat acccgggggt tcccgatcga
     1981 ctcggtgtag cagaggacgg tgcccggccg gatctccgcc agcatgtcct cgctgcggtg
     2041 cgaccgcgcg agcgtgacgt cgacccccca gcgcggcagg agcttcgtca tcgtggagaa
     2101 cgcgccgccg tagagatcgg gactggagac gacgtggtcg ccgctctgca cgagcgcggc
     2161 gagggtcgtc gtgatcgcgg ccatcccgct cgagaacgac acggccccgt caccgcgctc
     2221 gaggtcggcg agggcccgct cgagcgcctc gcgcgtcggg ttgttccagc gggtgtagac
     2281 gtgaccggcg tccttcccct gccggatcca ctcggcgagg acctccgcat cgtcgaaggc
     2341 gaaggtcgcg gtctggtaga tcgggaccga caggggctcg gccccccgcg cgtcggcggc
     2401 cgctcggagc gaccgggtcg tgaagccccg ctcttccacg cggccagcct accggacgga
     2461 cccgagccgg ggcccgctac cagggggccg ggtcgcagag ggcgcgttcg aggaccgcgc
     2521 ggggaaggtt cccgccggag aggatcgtgc cgaccctctt ccccgcgaga cggtcggcca
     2581 gcttcaccac gccggccgtg ccggcagctc ccgctccctc cgcgaggttg tgggtggcgt
     2641 ccagcaacag gaggatcgcc tgccgcagct cctcctcggt ggcgtccacg aggtcgtcga
     2701 cgtgccggtc gatcatcgcg aacggaaggc tcgtcggcac ccgcgccgcg agcccctcgg
     2761 cgaacgtctc ggccgatgga gcctcgaccc gccgcccctc ccgccgagag gcgaggtagg
     2821 gggcgcaggc cttcgcctgg accccgatga cctccacttc cggccggatc gctttcgcgg
     2881 cgagcgccac gccgctgatc agccccccgg cgccgaccgg gaccacgagc gcgtcgaggt
     2941 cggggacggc ctcgagcatc tcgaggccga tcgtcccgtg ccccgcgacg atccacgggt
     3001 cgtcggccgc gtggaagtag acgagaccct tctcggcctc gagctcgcgg gccttcgcgt
     3061 cggcttcgtc gaagtcgcgt ccttcgaaca cgatctccgc ccccaggtcg cgcatcgccg
     3121 cgaccttgtc cgggttcgcc gactcgggca tcacgacgat cggatggacg tcgacgagcc
     3181 gcgccgcgta gcacagaccc tgcccgtggt tccccatcga ggcggtgacg acgccgcgca
     3241 gccgctcctc cgggtcaagg gagaggacct tgttgatgtt gccgcgcacc ttgaaggcgc
     3301 cgaccggctg atggttctcg tacttgatcc acaggcgaag gccgagctgt cgcccgagcg
     3361 cgatgctctc gcggaagggc gtccgcggca ggtgcgcgtc gatcagccgg cgagccgcga
     3421 ggatgtcccc gagggagggc tcgggcgctc gctggtcttc cgtcacgacc actccttcgg
     3481 gtaccgggcg cgaagatcgt cgacgaggac cgacagctcc gacatcacct cgtcggtgac
     3541 gcgccggagg acctgcgggt gatcgggatc ctcgtcctgg agcgagaagc tgagcgggcg
     3601 ccccgccttc acccagatcg ggcggccgaa ccgaaggctg cgcgtgccct cacgctgcca
     3661 gacgtgctgc gagccccaaa ccgccagggg gatcaccggg acgccggtcg ccagggtgag
     3721 ccgcgcgatc ccggtcttgc ccgccatcgg cgagaagtcg tcgttcttcg tgacggtgga
     3781 ctccgggtag accatcacga cctcccccgc ggccagcgcc ttcagcgcgg cctcgacggg
     3841 cgcgcgctcc cccgacccgc gccggacggg gatctgccgg gcgccgcgga gcacggcgcg
     3901 cagcagcggg tttcggtaca gctcgatctt cgccatgaat cgcgggcggc gcttcccctt
     3961 ctccacgaag tagccgtggc agagcgggtc gaggtaggag atgtggttgc aggcgacgag
     4021 agccggcccc tgccgcggga ggtgctcgag accctcgaac cgccacttga accatgtcca
     4081 cagcggtggg atgcagacgc tctccgccag gcgcatccat ggctccggcc gcttcacgaa
     4141 ccctcctcct cgcgcggtcg ggacattcta cggatgcgtg aatcacatcg gtgtcgccgc
     4201 tagcatcggt cccctggtgc ggatctgcgc ggtcccggtg aaggcgctcg agcgagccaa
     4261 aggtcggctc gccgacacgc tcgcgcccct cgagcgggcg gcgctcacgc tcgcgatgct
     4321 cgaggacgtg ctcgacgcat gccttcggct gcccggatgg cacgtggtcg tgatctcgcc
     4381 agaccaagcg gtcctcgagg tcgcggcgaa acgcggcgtg cgaccactcg aggaggagga
     4441 gccgggcctc gtcgcggcgg tacgccaggt cgaggaggcg ttcccgacgg cggagcaact
     4501 cctcgtggtc ctcggcgacc tgccgctcct gacgggcgag tcgcttcagc gggtggtgcg
     4561 cacgctcggt ccggtcgtgg cggccccgtc gagctcggac gccgggacga acgtcctgct
     4621 gcgacgcccg ccggggatcg tgccgcccct gtacggaacc gcgtcgttcg agcggcaccg
     4681 cgaagcggcc gagctgcgcg gcgtcccgtt ctcggtcgtg cgcgcccccg agctgcagtt
     4741 cgacctcgac cgcggggagg acctcgcaca gctcctcacg tggccgcgtc cgacccggac
     4801 gaaggcggct tgcctcgaga tggggcttcc ggacagactg aacctccgcg tgccgtagag
     4861 ggaggagccg tgccgcaggg aacgatcaag gagttcgaca agggatcccg gaccgggatc
     4921 ctctacctcg acgacgagag cgaggtccag atcgacgcga gctccgtcga gggggcggac
     4981 atccagcacc tgcggatcgg tcagcgcgtg aagttcgacc tcgccgagga aggcggtcgc
     5041 cggctcgcgc gcgtcctgcg gatcgtgacg ttcgagtagt cagcgcctgc gggccgccgt
     5101 tcgggtggtc cgcttccgac gcttcggcag gaccgcctca cggaacagct tccccggccg
     5161 gaacgacggg acggtcgtcg cggcgatcgg gaccggctcg ccggtgtgag ggttgcgtcc
     5221 gagccgtgcg gctcggcgct gcttctcgaa ggtcccgaag cccgagagcg tcacccgctg
     5281 ccccttcgcc acgctttccc ggatcgccga gatcatcgcc tcgaccgcgt cggccgcctc
     5341 gggtggctcg agtcccgtcc ggcgcgcgac ctcgttcacg aggtcctgct tgttcacgtc
     5401 gcgagtctac gtcgtcgcgc tgcggccgcc ccggccgctg agtcgcggcc acgagagcag
     5461 ggcgtcgacg ccgtcggagt agtgcacgag cggcgccacc tccggccgcg ggaaccccgc
     5521 gcgatccgtc agcgtctgct cgatccgtcg tgcggtgacg cgacggaaca cccaccgtgg
     5581 gtgctcgacg aggagtccgg cggagatcgg tccgagcccc gtgtagagca cccagcgcgc
     5641 ggtgaggaag tgatcgaaac gtgtcagctc gtcggcgggg atcgcggcgc ccaggtcgag
     5701 gtcgagccgg aggcctcccc cgccggagct gcggcgtcgc gtccggtagc gggccctgtc
     5761 cgcccccgct cggacctcgt tcgtcgccca ccggtacggg aaccacatcg cgcgtccgag
     5821 cgccgccgcc tggagcggcg ccatgtcgag cgagaacatc cagatgccgc gccgcccgtc
     5881 gggcccgcgg acgtaggtcc ggaggtgcga ctcgtgggcg gtgcgcaagc ctgggatggg
     5941 tgggagccgc cggcccgggt tcatgtgggc gatccggaaa gagaccagac cgacccaggc
     6001 gcggccgtcg tggaggtcca gctcgaacgc actcggcacg tgcggccgga tcgcctccgg
     6061 gtcgtacggc cagtgcagga acgcgcagtg ccgccacgcc tgcgacagga gcggccgctc
     6121 gatccggacc ggcggatccg gcgcgatcca gtcgatcgcg gcggcgggct ccgagctcgt
     6181 cggcggcatg ctgcacgcct aaccgctgga accggaaccg aaaccacgca ccgcggggcg
     6241 cgatcgggca tcgggaccat gccaggacgc gcccgcctcc ggacgggtat catccgacga
     6301 gcgcatcggt gggtcgtggg gtaacggtag cccggctgac tctggatcag ctagtccagg
     6361 ttcgaatcct ggcgacccag ccaggtccct tcgtctagtg gcctaggaca ccaccctctc
     6421 aaggtggaga cacgggttcg aatcccgtag ggaccaccgc acccgacatt tccctcggtt
     6481 ctccgaccgc gtcgcccgct gtctccaccc aagaggtccg cgagggaggt ccgaggccgc
     6541 gacgtcccga ggagccacga tgacagggag ccgatcgatg ccctgtggac gaccgccggg
     6601 accctacttc gtttccggta tcgcccggat gggtaggagc cttttccccg gcccccagac
     6661 gcgatgcatc cgatcctttc gatacgacgt cccgccacgc tcatcatcgc gaccgccatc
     6721 gccgtggggt ccctccaagc cgttccggat ccggccgtgg ccatcacgca ggacggaacg
     6781 gccatggcga ggctgatcaa cgccagccgg gccgagcggg gtcgtcgcac cctccggatc
     6841 tccgagcggc tcagctccgt ggctcagaag catgcgtcgg ccatggcgag cgctggccgc
     6901 ctttaccaca acccgtcgct cgcgcagcag ctcagcacgt tcaactggac gatcctcggt
     6961 gagaacgtcg gtgtcggggc gaccgttccg accctgcacc gcgcgttcat ggcttcggat
     7021 gcccatcgga ggaacatcct gcggaggccg ttccggcggg tgggcgtcgg aacggtcatg
     7081 gcggggggac ggttgtgggt cgtggtcgtc ttctcggact gagtcgtcgc agcggggcgc
     7141 ggggggctag cggaacaacc tgcggaggag acgcccggtg gccttccccg cggcgcggcg
     7201 cccgatgcgg cggcccgccc tgcctttctg aaccgcgttg acgtcgccga gcagtcgcgc
     7261 cagtcggtag aggaaactgc ggaacccatt gaggctcata ggcgcactgt agcgcgccgt
     7321 gacgacctcg tgacggtccc gggctcaggt gttcaagcac ccacccggcg gggtaggcgt
     7381 cccggcgaaa ggaggtcaag catgagtctg ctcgtgatcc tgatcatcct ggcgctggtc
     7441 gccggcggcc tggggctcct cgtcgagggg ctgaagtggc tgctgatcat cgccgcgatc
     7501 ctgttcatcg cggggatctt caccgggttc cgcgggcgga gccgggtcta gcccggttcc
     7561 gcgctgaggg gctcggggcc cggaacgatc ccagcccgcc ggaggacgtc gtcgaggtcg
     7621 gccagcgtgc cggcctcgac gtctgcgggg cgacgggtca ccgcggtggc gatcacgccg
     7681 cggcgccgga ggatctcctt ccggatcccg agcccgatca gcggctgagc ctcgaactgg
     7741 atcaggggga ggtaccggtc gaagatccgc gccgcggcct cgtgctcccc cgcttccgtc
     7801 ccgcgccgga cggcggagag gatctccggg aaggcgaacc cggtcatcgt gccgcaggcg
     7861 ccgcggcgca gctccgagag ggccatcacg ccccccaggc cgccgaagac gtcgaggccg
     7921 ggacgcgccg agagcagccg cccgatcttc ggcggcgtcg gcgggtcctc gagcttgacc
     7981 gtcgcgcttc ccgcggcatc gaggcaccgg aggaggatct cgaggtccat ccgcacaccc
     8041 gtcgccgcgg gctcgtcctg gacgacgatc ggcaggccac cccggtcgag gacggcgccg
     8101 tagatgcccg ggaggccctc ggggtcggag aagcccggcg gggcggagac catcgcggcg
     8161 gccgcgccgg cgtcggctgc cgcggtcgcc cgcgccacga tccggtcggg gtcgggcgcc
     8221 gagcatccga ccaccaccgg gacgcggtcg ccgacctcct ccgcgacggt ccgcaggacc
     8281 cggtcacgct cctcctccgt cagctccgcg gcctcgctcg tcacgccgag caccgtgagg
     8341 ccgtcgaccc cccaggtgat ggcggcatcg agcaggcgcc gctggctctc ctcgtcgagc
     8401 gagccgtcct cgcggaacgg ggtggggacg acgaaccagg tcccgcggag cctcacagga
     8461 cccccgtgtg cggcacggcg aactcgcggt cggggcggaa cgcgttcgtg cggtcgcgcc
     8521 cgacgatccc gtcgacgacg aggctgctgg cggccgggct catcatcacc ccgtgcccgc
     8581 tgtagccggt gttcacccag aggcctggca gggccgtctg cccgatcagc ggccgacggt
     8641 cgggggtcat cgtgtactga ccggcctgga gcacccagtg gtcgctgcca cgctcccaga
     8701 cgctgtgcca gaacggagcg atccgtgcga gcgccgccgg gctgcgcgga tccagcacgg
     8761 agaacgcgaa gctcggatcc ggcgtcacga cctcctccgg gggggacgcg ggggtggtcg
     8821 gatcggtgaa cagcgcgaac gcgccgcgga acgccgggcg ccagtgggcg ccggtgtcct
     8881 cgtcgatcgt catcggcgcg tcctgtggca cctccgggac ctccggcagg acgagcttgt
     8941 gccggcggac cgtgtggatc ggaagatcga tccccacccg ctcggcgagc cttcccgaca
     9001 gcgggccaca ggcgatcacc gcgttgcgcg tcgagatcgt cccacgctcc gtctcgacgc
     9061 cgaccaccgc gtcaccctcg atgcggaacc ccgtgacacc acagcggacg acgacgtccg
     9121 ctccggacgc cgccgcgaga ccgaacgtga ccgccttcgg gtccacgagc ccgtcgcccg
     9181 cacggtatcg agcctgcacg acctccgggg tgatgaacgg gaaccgtcga actacctctt
     9241 cgcgatcgag cacctcgatc ccgtcgacgc cccacgacgc ctgcgtctcc gccaaccgac
     9301 gctgcccctc gaccgcgtct tcggaggtcg tgacccagag gtagccctgc tcgcgcacct
     9361 tcgggtcgta cacggcctgg ccggtccgct cggcgaaggc gtggaagagc tccaccgatc
     9421 cccggacgac gaggaactcc tcctcggtgt cgagctggag ccggaacccg ccggcggcgg
     9481 cggccgtcgt gagcgtgcag agcgcggggc ggcgctcgac gaggaccgtt cgaaggccgg
     9541 ccgcgctggc gtggaacgcg gtggccgcac ccacgatgcc gccgccgatc acgacgacgt
     9601 cggcggtcga tggcggatcg gacacgtagc ggaccggcac ccgcggcacg ataccaggcc
     9661 cgcgacgagc cgccgccggc taggtcgtct cgacctcgat cggccggagg gtgacgtcga
     9721 cggcgaggtc gttcgcgatg tcctcgatct cgcgctcgag cgctccggcg tcggcggcct
     9781 cgggcacggc gacctcggcg atcatcgcgt agaccggttc gtcggcgctg ccgaccagcc
     9841 ggcacgagag gtcgttgatg ttcgcgccgc gctccgtgag gagccgcgag aggcgcgcga
     9901 cgatccccgg ctgatccgac ccgtagacca cgaggtagtg cgtcgccgac gggttgctcc
     9961 gacggccggg gtccaggctc cggaccgtga cggtgacgcg cagcggccac gccgcctgcc
    10021 cgagcgcctt ctccagcgcg gtcgcatcca cgtcccgcgg cgcgttgacg acgagcatca
    10081 tcgcgaagtg cccgcggagg atcgtcgagg ccacgtcctc caggttgccg ccgatcccgt
    10141 acagcgcctc ggtgagcgcc gccacgatgc ccggccgatc ggcgccgatc gccgtcaccg
    10201 cgaagtgcgc cacgctcagt cccggagcga gcgcaggcgc gcgagggtgc ggtcccggcc
    10261 gagcagctcg atcgattcga acagcggggg tgagacgagg gtaccggtca cggctgcccg
    10321 gacgggctgg aacgccttcc gcgcgccgag ctctcgctcc tcctggagct cgcggaggac
    10381 cccctcgatc ccagccgccg accacggttc gaccttctcc agtcgcgtcg cgacctccga
    10441 gaggtactcc gtctgcccgg cgagcatctt cgccgccttc tcgtccggct cgacctcctc
    10501 gaacaggaac cggaggagcc cgggtgcctc gctgagcagc ttcatgcgct ccttcacgag
    10561 tggcaccgcg ggatccaaca gcgatcggtc caccgccgtg ccgcccgagg cgagtctctc
    10621 ctcgatcagg tccccgagcc ggtcgtcggc cgcctcgcgg atgtagtggc cgttcatcca
    10681 ctcgagcttc tctcggtcga aggccgcagg gttgtgggag acccgcgcga ggtcgaaccg
    10741 ctcgatcagc tcctcccgcg tgaagaaggt cgtcttctcg tcgaaggac
//
LOCUS       DATLGG010000317         4694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_107879, whole genome shotgun sequence.
ACCESSION   DATLGG010000317 DATLGG010000000
VERSION     DATLGG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107879"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1982
                     /locus_tag="VM638_09035"
     CDS             <1..1982
                     /locus_tag="VM638_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVM12599.1"
                     /translation="GIPHAVGVFRGREAGGFHPSVTTEADDIIRSVEVVDLNRDGSSD
                     VFAGVGWMGAQAEQRAVLFPGAGDGTLGPPLELGLPPGRAVTAGDLLDGSAAVAIATA
                     EEIQIHRSDADGVVRSERAIEAPGVTWLDAADLDADGTDELLAWSEEAQELSLFTATG
                     ERSWIHVSGGRSFRAADATGDGRAEILAGTGDGTVLVLDRDLDTIRTVAGSASDVHAV
                     DAADLDGDGSPEVVSARAGVVEVAHADGRRTTTRSSDSPWTLLLHDVAGDPAVDVVIA
                     LPTMDAVEILVNDGSGGFAAPTRTVPTEVYSWYLDHGDFNGDGDADVLASQSDPFLNY
                     QKPQAATVFLGDGGGGFQPSAELETTGAPVGTGVGDFDGDGDQDVLVGDQLGYLSQFF
                     GAGDGTFGARQPGPGCLLPMNVATADLDADGYDDAAAICRNVGGIGPDQVITLFGSPV
                     GLVRGQQLVLPNAWHGAIVRIGDVNGDGLRDLVVGSDMHNPPTCTDCFLYGFGKAVVT
                     TFLGLPTGRAWEPVGREHPVTEAISDIAVADLDGDGRDEILASLPFAGEVRVTPVLSD
                     GTFAQSYDVDTYARPGRIEIADLEDDGLLDLVLSHEQHSAVSVARGGPNGFGPPTAFM
                     TNGRVRVVHAVDMSGDDRLDLLGGRIDAFDILLQR"
     gene            2116..4248
                     /locus_tag="VM638_09040"
     CDS             2116..4248
                     /locus_tag="VM638_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12600.1"
                     /translation="MTLRLGSGRRLRAAGVLLAVPLLLAGATSAQAYDPPGRTERVSV
                     PSDGGQADFVSFRPSISADGVRVAFQSSASNLVPGDTNGWGDIFVRDRKMNTTRRVSV
                     SSDGSQANRTSTAPAISADGRYVAFISGATNLVPGDTSTAEDVFVHDLATARTERVSV
                     SSEGAEANAPAVASDGNVPAISADGRYVTFVSAASNLVPGDSNELADVFIRDRILGAT
                     ERVSAGIAGVDSNGASGQPSISADGRLVAFQSTATNLVEGDTNLWEDAFVRDRSTGTT
                     ERISVGAAGAQGNHRSGWPSISADGRHVAFQSFASNLVPNDTNGLRERLEDRGWDIFV
                     RDRTAGTTERVSVGNDGAEADQASRQLAISGDGRHVAFQSQATNLASGDTNAVLDVFI
                     HDRQTRQTERLSRSSGGAQGNGHSQWPTLSADGRFVVFESGATNLVPGDSNGFGDIFV
                     RDRGPGTGIGGVTVTVNAAQRLSLDGWARFAGIDLTSAADPAVDASAGAGSLGGELTG
                     AGLTLRPENEDLLAGWMLSSLPATMTGSLPMVAYGLRFTLDGVAYEVRSLRTSTDAEP
                     PGAPHFVLLRCASTCEERGRLRGGIGTVGSSVLVSIPLSAIGAEGGSRLTDIVAFTAL
                     AEARSERMATFDEVVLAQSQIQPAQVEAGAASDGHGEDAVAFSSVSGLSSGRFTATLD
                     PPGGSGGSVWTRACLGAICAPAHGQAIP"
     gene            4275..>4694
                     /locus_tag="VM638_09045"
     CDS             4275..>4694
                     /locus_tag="VM638_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12601.1"
                     /translation="MDRRQAPRRVATMLLTTLVIGTLASPASAYPRPGQTERVSIRSD
                     SSQGNSASSSPAMSADGRFVAFASVAYNLVPQDTNATRDIFVHDRHRGTTERVSISSG
                     GVQANGPSYNPAISADGRHVAFESTATNLAPGDGNQLS"
BASE COUNT          729 a         1647 c         1628 g          690 t
ORIGIN      
        1 gaggcatccc gcacgcggtc ggggtcttcc gcgggcgtga ggccggaggg ttccaccctt
       61 ccgtcaccac ggaagccgac gacatcatcc gctccgtcga ggtcgtggac ctgaaccgtg
      121 acggctcctc cgacgtcttc gccggggtcg ggtggatggg tgcccaagca gagcagcgtg
      181 cggtgctgtt ccccggcgcc ggcgacggca cgctcggccc gccgctcgag ctcgggctcc
      241 cgccggggcg cgccgtgaca gcaggcgatc tcctcgacgg ctccgccgcg gtcgcgatcg
      301 cgacggcgga ggagatccag atccaccgat ccgacgcgga tggcgttgtc cgatccgagc
      361 gggcgatcga ggccccgggg gtcacgtggc tggacgcggc ggacctggac gccgacggca
      421 ccgacgagct gctcgcctgg tcggaggaag cgcaggagct gagtctgttc accgcaaccg
      481 gcgagcgaag ctggatccac gtttccggag gacgctcgtt ccgcgccgcg gacgccacgg
      541 gtgacgggcg ggcggagatc ctcgccggga ccggcgacgg gaccgtcctg gtcctcgacc
      601 gcgatctcga tacgatccga acggtggcgg gatccgcctc cgacgtccat gcggtggacg
      661 cggcggacct cgatggggat ggatccccgg aggtcgtctc ggcacgtgca ggtgtcgtgg
      721 aggtcgcgca tgccgacgga cgacggacca cgacgcgctc ctccgacagc ccgtggacgc
      781 tcctgctcca cgacgtcgca ggcgatccgg cggtggatgt ggtcatcgcg ctcccgacga
      841 tggacgcggt ggagatcctc gtgaacgacg gatccggcgg gttcgcggcg cccaccagga
      901 cggtcccgac cgaggtctac tcctggtacc tcgaccacgg cgacttcaac ggggacggcg
      961 acgccgatgt cctggcgtcc cagtcggatc ccttcctcaa ctaccagaag ccacaggctg
     1021 caaccgtctt cctcggcgac ggcggcggcg ggttccagcc gtcggcggag ctggagacga
     1081 ccggcgctcc cgtcgggacc ggcgtcggcg acttcgacgg ggatggtgat caagacgtcc
     1141 tcgtgggaga ccagttgggc tacctgagcc agttcttcgg cgcaggcgac gggacgttcg
     1201 gggcacgcca gcccggaccg ggctgcctgc tgccgatgaa cgtcgcgacc gcggacctcg
     1261 acgccgatgg ctacgacgac gcggcggcca tctgccggaa cgtgggcggg atcggtccgg
     1321 atcaggtcat cacgctgttc gggagcccgg tcgggctcgt ccggggccag cagctcgtcc
     1381 tgccgaacgc ctggcacggg gcgatcgtgc ggatcggcga cgtgaacgga gatgggctgc
     1441 gcgacctggt ggtcggatcg gacatgcaca acccgccgac ctgcaccgat tgcttcctct
     1501 acgggttcgg caaggcggtc gtgaccacct tcctcgggct gccgacggga cgcgcctggg
     1561 agcccgtggg gcgcgagcac ccggtgacgg aggcgatctc ggacatcgcc gtagcggacc
     1621 tcgacggaga cggtcgagac gagatcctgg cctccctgcc gttcgccgga gaggttcgcg
     1681 tgacgcccgt gctcagcgac gggacgttcg cgcagtccta cgacgtcgat acctacgcgc
     1741 gccccggccg catcgagatc gcggatctcg aagacgatgg gctgctcgac ctcgtcctca
     1801 gccatgaaca gcacagcgcc gtgtcggtcg cccggggtgg gccgaacggg ttcggcccac
     1861 ctaccgcctt catgacgaac ggacgggtcc gcgtcgtcca cgcagtcgac atgagcggcg
     1921 acgaccggct cgacctcctc ggcggccgca tcgacgcgtt cgacatcctc ctccagcgct
     1981 gacgggacgc gacggcgcag cgcgccacgg ccccgaagat gcggctttgt gcaggtgcac
     2041 gaagggaccc ctgcatcgcc ggacgaatac cactggtatg cctctcgctc tccgcgctcc
     2101 cgatcggctc cgcccatgac ccttcgcctg ggttcgggcc ggcgcctgcg cgcggcgggc
     2161 gtgcttctcg ccgttcccct cctcctcgca ggcgcgacaa gcgcgcaggc gtacgacccc
     2221 cctgggcgaa ccgagcgcgt atcggttccg tcagacggcg gccaagcgga cttcgtctcg
     2281 ttccgtccct ccatcagtgc cgacggcgtt cgcgttgcgt tccagagctc ggcgtcgaac
     2341 ctcgtgccgg gggatacgaa cggctgggga gacatcttcg tgcgggaccg gaagatgaac
     2401 acgacgcgcc gcgtatccgt gtcgtcggat ggatctcagg cgaaccgcac ctcgaccgcg
     2461 ccggcgatca gcgccgatgg ccggtacgtc gctttcatca gcggcgccac gaacctggtc
     2521 cctggagaca ccagcaccgc tgaggatgta ttcgtccacg acctcgcgac ggcgcgcacc
     2581 gagcgggttt cggtatcgag cgagggagcc gaagccaacg ctcccgccgt ggccagcgat
     2641 gggaacgttc ccgccatcag cgccgacggc cgctacgtca cattcgtcag cgcggcatcg
     2701 aacctcgtcc ccggagacag caatgagctg gccgatgtct tcatccggga ccgcatcctt
     2761 ggagccacgg agcgggtctc ggcggggatc gcaggtgtcg acagcaacgg tgcatcgggg
     2821 cagccgtcga tcagtgcgga tgggcgactc gtcgccttcc agagcacggc aacgaacctg
     2881 gtcgagggcg acaccaacct gtgggaggac gccttcgtgc gtgatcgatc gaccggaacg
     2941 acggagcgca tctccgtcgg cgctgccggc gcccagggca atcatcgctc cggctggccg
     3001 tccatcagtg ccgacgggcg ccacgtcgcc ttccagagct tcgcctcgaa cctcgttccg
     3061 aacgacacga acggcctccg cgagagactc gaggatcgag gatgggacat cttcgtgcgc
     3121 gaccggaccg ccgggaccac cgaacgcgtc tcggtcggga acgatggcgc tgaagccgac
     3181 caggcatccc gacagctcgc gatcagcggc gacgggcgcc acgtcgcctt ccagagccaa
     3241 gccaccaacc tcgcgtccgg cgacacgaac gccgtgctgg acgtcttcat ccacgaccgg
     3301 cagacgcgcc agaccgagcg cctctcccgg tcgagcggag gcgcgcaggg gaacggtcac
     3361 tcccagtggc cgacgctgag cgccgacggc cgcttcgtcg tcttcgagag tggggcgacc
     3421 aacctcgtac ccggggactc gaacgggttc ggcgacatct tcgtgcggga tcgcggaccg
     3481 ggcacgggga tcggcggcgt gacggtgacc gtcaacgccg cgcaacggct ttcgctggac
     3541 gggtgggcgc gattcgccgg gatcgatctc acgagcgccg cggacccggc cgtggacgcg
     3601 tctgcgggcg cggggtccct cggtggagag ctgacaggcg cggggctcac gcttcgcccc
     3661 gagaacgaag acctcctggc ggggtggatg ctctcctcgc tgccggccac catgactggc
     3721 tctctgccca tggtcgccta cggcctgcgg ttcaccctcg acggagtggc atacgaggtg
     3781 cggagccttc gcacttcgac cgacgccgag ccccccggtg cgccgcattt cgtgctcctg
     3841 cgctgtgcct cgacgtgcga agaacgaggg cgactccgcg gtggcatcgg cacggttgga
     3901 tcgagcgtcc tggtctcgat cccgctcagc gcgatcggcg ccgaaggagg gagccggctg
     3961 accgacatcg tggcgttcac cgcgctcgcc gaagcgcgga gcgagcggat ggccacgttc
     4021 gatgaggtcg tcctcgctca gagccagatc cagcccgcac aggtggaagc cggtgccgcg
     4081 tccgatggtc acggagaaga cgcggtcgcg ttctcctcgg tgagcgggct ctcgtccggc
     4141 cgcttcaccg ccacactcga tccaccgggc ggctccggcg gctccgtgtg gacgcgcgcc
     4201 tgcctgggtg cgatctgcgc gcccgcccac ggacaggcca taccatgagg gcctacgcgt
     4261 tcagggccca cagcgtggat cgacgccagg caccccggcg tgtcgcgacg atgctcctca
     4321 ccacactcgt catcggcacc ctcgcctcac cggcgagcgc ctacccgcgt cccggacaga
     4381 cggagcgggt ctcgatccgc agcgacagta gccaggggaa cagcgcatcc tcgagtcccg
     4441 cgatgagcgc cgacggcagg ttcgtcgcct tcgcgagcgt cgcgtacaac ctcgttccgc
     4501 aggacacgaa cgcgacgcgc gacatcttcg tccacgaccg gcaccgtggg acgaccgagc
     4561 gcgtgtcgat ctcgagcggc ggcgtgcaag cgaacgggcc gtcgtataac cccgcgatca
     4621 gcgccgacgg gcgccacgtt gcgttcgaaa gcaccgccac gaacctcgct ccaggagatg
     4681 gcaatcagct gtct
//
LOCUS       DATLGG010000318         2522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_219532, whole genome shotgun sequence.
ACCESSION   DATLGG010000318 DATLGG010000000
VERSION     DATLGG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219532"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VM638_09050"
     CDS             complement(<1..913)
                     /locus_tag="VM638_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVM12602.1"
                     /translation="MRQAPELAIDLGTANTLVFQKGRGIVLNQPTVVALNARDGEVLA
                     MGEEAWDMIGRTPAQIVAVRPLRHGAVTDYDVTAQMLRIILKTVGVTRWTKPRTMVCV
                     PSAITDVERRAVEEATRAAGARSVTLIEEPMAAAIGAGLPIHEPIGNLIVDVGGGTSE
                     VAMVSMGGLISKRSVRIGGFDMDAALQQHLRREYNIAVGERAAEHLKITAGTAYPLPD
                     STRAEVRGRDVTTGMPKSVQLSEEEIREALAEPVEAIVRAARACLSESPPELGHDVLE
                     RGMFLCGGGALLRGLDMRISQECEVPVH"
     gene            complement(962..1831)
                     /locus_tag="VM638_09055"
     CDS             complement(962..1831)
                     /locus_tag="VM638_09055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVM12603.1"
                     /translation="MPRIRRAEEGDLPDAAEIFAAACGRLIEEYHPADRPYLPLDPAE
                     RLPMYRHLLGTGAFFIAEDPGPVGFASAVIRDGVWFLSQLWVHPDHQAGGLGGALLDE
                     ALEWGRGCSAFAVLSSVHPGARTLYLRRSMFPRWEENDMGGDGGGDAPPGTRPLHADD
                     DAWVAELDRVARGFARPEDHALWRRTGTGVAVERDGVPLGYAYVGSGPGGKRRVGPGA
                     VRHPRDMPVLLRAALAAIGSGPVRVQVPSTNAAAVNELIRLGFRWYGSSAFLASRPLS
                     DGSTYLSSGGALG"
     gene            1874..>2522
                     /locus_tag="VM638_09060"
     CDS             1874..>2522
                     /locus_tag="VM638_09060"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HVM12604.1"
                     /translation="MADSTPTGGSPDPFADTPLFREIQRILQSGGGPVNWELARQVGI
                     ASARGGGEAPIGEDEAARLEGAVRMAEIAIGDLTGLPGEVLRVRAVDRGDWVRANADG
                     LRDLFEAPAARVSALLADARRGEQDLPLEGGPMLEAMLGQLSPLLMGAQVGTVLGRLA
                     QDVLTQYELPLPRAGAPELLFVAPNIETFERDWSLPREELRGYLAMHETAHALTLG"
BASE COUNT          342 a          913 c          933 g          334 t
ORIGIN      
        1 ggtggaccgg cacctcgcat tcctgggaga tccgcatgtc gagcccgcgc agcagcgcac
       61 ccccgccgca caggaacatc ccgcgctcga ggacgtcgtg gccgagttcc ggcgggctct
      121 ccgacaggca ggcgcgcgcc gctcgcacga tcgcctcgac cggctcggcg agcgcctcgc
      181 ggatctcctc ctccgacagc tggacggact tcggcatccc ggtcgtgacg tcgcgccccc
      241 gcacctcggc gcgcgtggag tcgggtagcg ggtacgccgt tccggcggtg atcttcagat
      301 gttccgccgc acgctccccg accgcgatgt tgtactcgcg ccggaggtgc tgctggaggg
      361 ccgcgtccat gtcgaagccg ccgatccgca ccgagcgctt cgagatcaga ccgcccatcg
      421 agaccatcgc cacctcggag gttccgccgc cgacgtcgac gatcaggttc ccgatcggct
      481 cgtggatcgg caggcccgca ccgatcgcgg cggccatcgg ctcctcgatc agggtgacgg
      541 agcgcgcgcc ggccgcgcgc gtcgcctcct cgaccgcgcg acgctcgacg tcggtgatcg
      601 cagagggaac gcagaccatc gtgcgcggtt tcgtccacct cgtcaccccg acggtcttga
      661 ggatgatccg cagcatctgc gccgtgacgt cgtagtcggt gacggccccg tgccgcagcg
      721 ggcggacggc gacgatctgc gcgggggtgc gtccgatcat gtcccacgct tcctcgccca
      781 tcgcgaggac ctcaccgtcg cgcgcgttca gggcgacgac ggtcggttgg ttgaggacga
      841 tccccctgcc cttctggaag acgagcgtgt tcgcggtgcc gaggtcgatc gcgagctcgg
      901 gcgcctggcg catcaggcgg ggaggggaag ggggagcggc atcgacgtca tcgtaccgcg
      961 ctcatccgag cgctccgccg ctggacaggt aggtcgaccc gtcggacagg ggacggctcg
     1021 cgaggaacgc gctgctgccg taccagcgga acccgagacg gatgagctcg ttcaccgccg
     1081 cagcgttcgt cgacgggacc tggacgcgga cggggccgga gccgatcgcg gcgagcgccg
     1141 cccggaggag aacgggcatg tcgcgcgggt ggcggacggc gccgggcccg acgcggcgct
     1201 tgccaccggg accggagccg acgtaggcgt acccgagcgg cacgccgtcg cgctcgaccg
     1261 cgaccccggt cccggtccgg cgccagagcg cgtggtcctc gggacgagcg aacccgcggg
     1321 ccacgcggtc gagctcggcg acccatgcgt cgtcgtccgc gtggagcggg cgcgtgccgg
     1381 gcggcgcgtc gccgccgccg tcccctccca tgtcgttctc ctcccagcgc gggaacatcg
     1441 agcgacgcag gtagagcgtg cgcgctcccg ggtggaccga ggagaggacc gcgaacgcgg
     1501 agcaccccct cccccactcg agcgcttcgt cgagcagcgc gccgccgagc ccgcctgcct
     1561 ggtggtcggg gtggacccac agctgggaga ggaaccagac gccgtcgcgg atgaccgccg
     1621 atgcgaagcc caccggcccc gggtcctcgg cgatgaagaa ggcgccggtc ccgagcaggt
     1681 gacggtacat cggcaggcgc tcggcggggt cgagtggaag gtagggacgg tccgcggggt
     1741 ggtactcctc gatcagacgg ccgcaggccg ccgcgaagat ctccgcagcg tcgggcagat
     1801 ctccttcctc ggctctgcgg atcctcggca cgtgacctcc ggggcttcga gcgggcaccg
     1861 ctaggatagg gacgtggcgg actcgacgcc gaccggcggc tcccccgatc ccttcgccga
     1921 cacgccgctg ttccgcgaga tccagcggat cctccagtcg gggggcggcc cggtgaactg
     1981 ggagctcgcg cggcaggtgg gcatcgcctc ggcacgcggt ggaggcgagg cgccgatcgg
     2041 cgaggacgag gccgctcgcc tggagggggc cgtgcggatg gccgagatcg cgatcggcga
     2101 tctgaccggc ctgccgggcg aggtccttcg ggtccgtgcg gtcgaccggg gcgactgggt
     2161 ccgcgcgaac gccgacggcc tgcgggacct gttcgaggcg cccgcggcgc gggtctccgc
     2221 cctgctcgcc gacgcgcgcc gcggcgagca ggacctgccg ctcgaaggcg gcccgatgct
     2281 cgaggcgatg ctcggacagc tcagcccgct cctgatgggt gcgcaggtcg ggaccgtgct
     2341 cggccgcctc gcacaggacg tcctgaccca gtacgagctc ccgctccccc gggccggcgc
     2401 gccggagctc ctgttcgtcg ccccgaacat cgagacgttc gagcgcgact ggagcctgcc
     2461 gcgcgaggag ctgcgcggct acctcgcgat gcacgagacc gcgcacgcgc tcaccctggg
     2521 ac
//
LOCUS       DATLGG010000319         3024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_186432, whole genome shotgun sequence.
ACCESSION   DATLGG010000319 DATLGG010000000
VERSION     DATLGG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186432"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VM638_09065"
     CDS             complement(<1..597)
                     /locus_tag="VM638_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVM12605.1"
                     /translation="MSVDPAIRTRGLTKRYGDLTAVDRLDLEVRRGEIFGLLGPNGAG
                     KTTTILMLLGLSEPTAGTAEVVGLDPTRRPLEVKRHVGYLPDNVGFYGTLSGRENLRY
                     TARLNGLAASSADDRIDDLLEQVGLAEAGDRRVETYSRGMRQRLGIADALVKDPEVLI
                     LDEPTVAIDPEGVAEILALIRGLARDRGVTMLLSSHLLH"
     gene            complement(611..1756)
                     /locus_tag="VM638_09070"
     CDS             complement(611..1756)
                     /locus_tag="VM638_09070"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HVM12606.1"
                     /translation="MRKRVLAIVAAAALTLPLPASASHGGVHITTPYPGVTVQPGSSV
                     TFDLSVSGPPGRRAALRVTREPRGWRAVLRGGGFVVNAVTTARRARTVQLEVEVPSEA
                     RPGSYRLAVRASSGGASDTLGLSLRVGGAGEGAGSVELETEFPTLQGAPDTTFTYDVQ
                     LRNNTPQEGMFALEATGPEGWNVTATPSGQEQASTLSVAGGETGSLTVSADPPDDATA
                     GTYELQLRATGGGQEATLELEVEITGNYSLSLSTTDERLNADVAADRPTEVPMVVVNN
                     GTAPLNGVELSATPPTDWRVDFQPRVIESIEPGGRAQVIARVTPSDQAVAGDYLLTME
                     ARTGETSESIEFRTTVKASPVWGFVGLLLIAAALGGLGWVFRRYGRR"
     gene            1878..2702
                     /locus_tag="VM638_09075"
     CDS             1878..2702
                     /locus_tag="VM638_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HVM12607.1"
                     /translation="MPEVQALAERLHDALAGTALVRLDPLQFAALKTVDPPPERLIGR
                     PLTGVGRRGKYLTFDYGGLRLLVHLSQGGRVVLEEPPKATRPRGAVLRIRHERGPGVL
                     VREFGTERKAGWWVLAEGDEGPLDRLGPEPHGPAFETFVLSGDDARRIHTILRDQRTV
                     AGIGRGYADEILHRARLSPFAALAKLTPDERRLLLAATREVLDEALEQERRRTGGLPA
                     RLGDRFRIHHRHGDPCPRCGDDLRRVSYASHEVTYCPACQTGGKVLADRRLSRLVR"
     gene            complement(2705..>3024)
                     /locus_tag="VM638_09080"
     CDS             complement(2705..>3024)
                     /locus_tag="VM638_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12608.1"
                     /translation="ARARIKLGWVGEYSRVEEIRRRTRGAPIHDVDDFWRGRARFGYA
                     AVAELAAEKWVEPFWAGPRTYAYEWILLESDKKRSTWLDEREPPRGGEDLVRRFETWR
                     AER"
BASE COUNT          382 a         1114 c         1102 g          426 t
ORIGIN      
        1 gtgcagcagg tggctcgaca gcagcatcgt gacgccccgg tcccgcgcca ggccgcggat
       61 cagcgcgagg atctcggcga cgccctcggg gtcgatcgcg accgtcggct cgtcgaggat
      121 cagcacctcc gggtccttga cgagcgcgtc ggcgatcccc agtcgctgcc gcatgccgcg
      181 ggagtaggtc tcgacgcgcc ggtcgccggc ctccgcgagg ccaacctgct cgaggaggtc
      241 gtcgatccgg tcgtcggcgc tggacgccgc cagcccgttc aggcgggccg tgtagcggag
      301 gttctcgcgg ccgctcagcg tcccatagaa gccgacgttg tcggggaggt agccgacgtg
      361 ccgtttgacc tcgagcgggc gccgcgtcgg gtcgagcccc acgacctcgg cggtacccgc
      421 ggtcggctcc gagaggccga gcagcatcag gatcgtcgtc gtcttgccgg cgccgttcgg
      481 gccgaggagg ccgaagatct cgccgcgccg cacctcgagg tcgaggcggt cgaccgcggt
      541 caggtcgccg tagcgcttcg tcagcccgcg ggtccggatc gccgggtcga cgctcatccc
      601 gtcctccgcg tcaccgccgc ccgtagcgtc ggaagaccca gccgagcccc ccgagcgcgg
      661 cggcgatcag caagagcccg acgaaccccc agacggggga ggccttgacc gtcgtcctga
      721 actcgatgga ctcgctcgtc tcgccggtcc gcgcctccat cgtcaggagg tagtcgcccg
      781 ccacggcttg gtccgacggc gtcacgcgcg cgatcacctg tgcccgcccg ccaggctcga
      841 tcgactcgat cacccggggc tggaaatcga cccgccagtc ggtgggcggg gtcgccgaca
      901 gctcgacgcc gttgaggggc gcggtcccgt tgttcacgac gaccatcggg acctcggtcg
      961 ggcggtccgc ggcgacgtcg gcgttcagcc gctcgtcggt cgtggagagg ctcagcgagt
     1021 agttcccggt gatctcgacc tcgagttcga gcgtcgcctc ctggccgccg cccgtcgccc
     1081 ggagctggag ctcgtaggtt cccgcggtcg cgtcgtccgg ggggtcagcg gagaccgtca
     1141 gcgacccggt ctcgccgccc gcgaccgaca gggtcgaggc ctgctcctgc cccgagggcg
     1201 tcgcggtcac gttccagcct tccggacccg tcgcctcgag cgcgaacatc ccctcctggg
     1261 gggtgttgtt ccggagctgg acgtcgtagg tgaacgtcgt gtccggtgcg ccctgcaggg
     1321 tcgggaactc cgtctcgagc tcgacgctcc cggcgccttc gccggctccg ccgacccgca
     1381 gcgacaggcc gagggtgtcg gaggcgcctc ccgagctcgc gcggacggcg aggcggtagc
     1441 tcccgggacg ggcctcggag gggacctcca cctcgagctg gaccgtccgg gcgcggcgcg
     1501 cggtcgtcac cgcgttcacg acgaaccccc ctccacggag gaccgcccgc cacccgcgcg
     1561 gttcgcgcgt cacgcgcagc gcggcccggc ggcccggcgg gccgctgacg ctcaggtcga
     1621 aggtgacgct cgagcccggt tgaaccgtca cgcctgggta gggcgtggtg atgtggacgc
     1681 cgccgtggga cgcgctcgcc gggagcggga gcgtcagcgc cgcggcagcg acgatggcaa
     1741 ggacgcgctt cctcatcctt cctccatcag gtcgtgtcgt tgcatgcggc accggtggtc
     1801 gcaccggagc ggccgcattg tagaaagcga ccaggccgag gtgtcaaccc gcgcgtatcc
     1861 tcgatctcgt gccggagatg ccggaggtcc aagcgctggc tgagcgtctc cacgacgcgc
     1921 tcgcggggac ggcgctcgtt cgcctcgacc cgctccagtt cgcggcgctg aagaccgtcg
     1981 acccgccgcc ggaacgtctc atcggacggc cgctgaccgg ggtgggacgc cgggggaagt
     2041 acctcacctt cgactacggc gggctgcggc tcctggtcca cctctcgcag ggcgggcggg
     2101 tggtcctcga ggagccgccg aaggcgacac ggccgcgcgg ggcggtcctg cggatccgcc
     2161 acgagagggg acccggggtg ctcgtgcggg agttcgggac cgagcggaag gccgggtggt
     2221 gggtcctcgc cgagggcgac gagggtccgc tcgaccgcct ggggccggag ccgcacggtc
     2281 ccgcgttcga gacgttcgtc ctgagcgggg acgacgcccg gcggatccac acgatccttc
     2341 gcgatcagcg gacggtggcg gggatcggtc ggggctacgc cgatgagatc ctgcaccgtg
     2401 cgcggctgtc gcccttcgcc gcgctcgcga agctcacccc ggacgagcga cggctcctgc
     2461 tcgccgccac gcgcgaggtg ctcgacgagg ccctcgaaca ggagcgccga cgcaccggcg
     2521 gcctcccggc gaggctcggc gaccgcttcc gcatccacca ccgccacggc gatccctgcc
     2581 cccgctgcgg cgacgacctg cggcgggtct cctacgcgtc gcacgaggtg acctactgcc
     2641 cggcgtgcca gaccgggggc aaggtgctcg ccgaccgccg cctctcccgg ctcgtccgat
     2701 agggtcagcg ctccgcgcgc caggtctcga agcgtcgcac gaggtcctca ccgccgcgcg
     2761 gtggctcccg ctcgtcgagc caggtcgagc gcttcttgtc gctctcgagg aggatccact
     2821 cgtaggcgta ggtgcggggc ccggcccaga acggctccac ccacttctcg gcggcgagct
     2881 cggcgacggc cgcgtagccg aaccgtgccc ggccgcgcca gaagtcgtcg acgtcgtgga
     2941 tcggcgctcc gcgggtccgc cggcggatct cctcgacccg cgagtactcg ccgacccagc
     3001 cgagcttgat ccgcgcgcgg gcga
//
LOCUS       DATLGG010000320         7917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_108614, whole genome shotgun sequence.
ACCESSION   DATLGG010000320 DATLGG010000000
VERSION     DATLGG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108614"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VM638_09085"
     CDS             complement(<1..714)
                     /locus_tag="VM638_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12609.1"
                     /translation="MATEGGERSGPREALLLLRRNGDFRRLYGAQLISYAGDWFATVA
                     LLGLLFDITRSSALTVMVVFVQVIGFAILSPLGGYVADRFDRRRLMIVVDLVRTVLAL
                     GLLLIDSASGVWFALLLVGVMTGLGAFFEPAATAAVPNLVDAEELPAANVLVGAAWGT
                     MLAVGGALGGVVAATLGRDAAFIGNALSFFMSALLVMGIRRRLAEERDPSRPEAGVVA
                     AVVETLRYARRDHRVLALLV"
     gene            776..1072
                     /locus_tag="VM638_09090"
     CDS             776..1072
                     /locus_tag="VM638_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12610.1"
                     /translation="MSLAARAPAQSRRSPWLRLLAVSVAAGAAATYLAGTNAGLCTPE
                     PESPCATLVRMLSLRVGIVTMIATALALLTVAGLARTMRLDEERRLAEAGDLSD"
     gene            1144..1782
                     /locus_tag="VM638_09095"
     CDS             1144..1782
                     /locus_tag="VM638_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVM12611.1"
                     /translation="MAVPIPFTDNYSDRSSEAGYQFEFFCERCGNGYKSGFQPSALGI
                     GTKVMRGLGGMLGGGLGNASYAGDQVKDLTESQAKDKALQQAVQQVAPLFNQCHRCGQ
                     WVCKEICWNDEAGLCVNDAPKLGQELGAMQAQAQLQQVQEKVQQVDWTTDMNVERRQV
                     ARCPNCQAEAEPSAKFCGSCGQALSMPVTCGRCGTESPRGTKFCPECGNQLG"
     gene            1784..3040
                     /locus_tag="VM638_09100"
     CDS             1784..3040
                     /locus_tag="VM638_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12612.1"
                     /translation="MTAYRGQLRVGNGAPVAAAIEPNDSGVALSPEGGSPTELAYVDI
                     EDLHDADYVLRLTDHTGQTYELTMLGRAYGQVLEDVRKAREARLEKDLLLRGVGLQDT
                     IPGKLFGGEQPVPVRLRLYEDLLVAIPERGTMFGIPYAFVEEVVWDEELYQVRVRTDL
                     GEEHVFGHLGRRSEELRDELRRLIAALERRTAATLRALLPGSEPGLVTRLASMMRDGR
                     VVQRRRVDALAPQLWGALEDAVAGTPELRAAYDDLSSVAGWTAFGIKAVRPAAADGPA
                     DAPDPEPTGSGARADLWYLRPLAREGRPLNAAAQEVTSEPGHATYVFRLMEPARFASL
                     SGEALADEVERSLALLNRALLQLNFRREPIYLSDEQIAADERYARYRVALRKLPHLRR
                     ARASFLGRAAHDAGWRRRIDELAASA"
     gene            3101..4021
                     /locus_tag="VM638_09105"
     CDS             3101..4021
                     /locus_tag="VM638_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HVM12613.1"
                     /translation="MPGYTGELYILAFDHRGSFRKKLGVGSNPTLEETEKVVDAKRLI
                     FEGFQQAIADGVPREQAGLLVDEEYGADVARKARAEGFKLAMPVEKSGQDEFDFEYGS
                     DYGQHIEEFDPTFSKVLVRYNPDADPEMNRRQTARLKDLSDWLHERGRSYLFELLVPA
                     EPAQLEGVGGDEDRYDTEVRPGLMLRAIEELLGAGVEPDIWKIEGLDRREDCEKVSAL
                     VRSGGRTEVACVVLGRGADDAAVDHWLRQGAGVPGYVGFAIGRSIWGKPLSAYVAGDL
                     ERGDAARQIAANYRRFIEVYRGAQGATASA"
     gene            4081..4284
                     /locus_tag="VM638_09110"
     CDS             4081..4284
                     /locus_tag="VM638_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015320709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVM12614.1"
                     /translation="MAVIKTIDLVGVSTESWRDAASQALAEAAKTLRGIEGMEVLETS
                     AIVEGGEIKEYQTHVRIKFRIER"
     gene            4378..5652
                     /locus_tag="VM638_09115"
     CDS             4378..5652
                     /locus_tag="VM638_09115"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVM12615.1"
                     /translation="MPATILVGTQWGDEGKGKATDQLADQMDMVVRYQGGNNAGHTVR
                     AAGRTLKLHLIPSGILYPHITSVIADGVVVDPRVLLEEMDQLEAEGIGTERLIVSANA
                     HLIMPYHLELDRVTERYLGRSKLGTTKRGIGPAYADKAARIGLRVQDLTDEKIFRQKL
                     EVVLKEKNAILTKVYNRLPLDAERIVEEYRGYGERLASHIADTSGILHEAIRAGKRVL
                     LEGAQGTLLDLDHGTYPFVTSSNPVAGGALTGSGLGPGVVERVIGVTKAYVTRVGSGP
                     FPTEDEGEAGARMQEVGQEFGTTTGRRRRCGWFDAVLLRYAARINGLTDLFLTKLDVL
                     SGLPEVKLCVAYRYEGKSYEDLPPHQSIIHKAEPVYEAMPGWPEGLDEVRSFDALPSA
                     ARDYVERIAGTAGVPVRAVSVGPSREQTMEVR"
     gene            5654..6925
                     /gene="purD"
                     /locus_tag="VM638_09120"
     CDS             5654..6925
                     /gene="purD"
                     /locus_tag="VM638_09120"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HVM12616.1"
                     /translation="MKVLVVGGGGREHALAWALARSDGVERVYAAPGNVGIGSVATTV
                     PIAATDVPALVAFVERERIDLTVIGPEAPLVAGLVDELQARGAPAFGPTAAGARLEGS
                     KAWARDLCARHGIPAPSYRTFDEAPAALAYLDSIGAPTVVKADGLAAGKGVTVAATRE
                     EAARAVEDALVRGRFGAAGGRVVIEEHLRGREVSALAITDGRTVLPLVLAQDFKRALD
                     GDEGPNTGGMGAYSPVPFVDPDTEARLVKETLEATVAALAAEGVAYRGVLYAGLMLTE
                     DGPKVLEFNCRFGDPEAQAVLPRLASDLAPVLLAAAGGGLEGIRLAWRPEACVTVVAA
                     SGGYPADHPTGLPIEGLDDAAALEHVLVFHSGTARRDDRVVTAGGRVLAVSALGGSLS
                     AARERAYEALERISFDGMHVRTDIAKEAAGG"
     gene            6918..7391
                     /gene="purE"
                     /locus_tag="VM638_09125"
     CDS             6918..7391
                     /gene="purE"
                     /locus_tag="VM638_09125"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVM12617.1"
                     /translation="MVEENPLVGILVGSASDLPVLQQGTAILERFGIPHEIHVMSAHR
                     NPEQVDEYVRGAEERGILVMICGAGMAAHLAGAVAARTVLPVIGVPVGGGAMNGVDAL
                     YSTVQMPRGVPVATVAIGGAVNAAILAAQIIAIRDEGIRKALHEFKESLAEGFKL"
     gene            7423..>7917
                     /locus_tag="VM638_09130"
     CDS             7423..>7917
                     /locus_tag="VM638_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVM12618.1"
                     /translation="MIPRYTLPEMAAVWSDEARLRNWLRIELLACEAWARLGRIPAED
                     LQALSKASVTAARVEELERVTNHDVAAFVQAAAESVGEAGRWIHLGLTSSDVLDTGLA
                     LQLRDATDLLLRRLEELLGVVKDRALEHRDTLCVGRTHGVIAEPTTFGYKLAVWAFEL
                     ARDRE"
BASE COUNT         1180 a         2730 c         2948 g         1059 t
ORIGIN      
        1 tacgaggagg gcgaggacgc gatgatcccg ccgcgcgtac cggagggtct ccacgacggc
       61 ggccacgacg cctgcctccg ggcgcgaggg gtcgcgctcc tccgcgaggc gtcgtcggat
      121 ccccatgacg agcagcgcgg acatgaagaa cgacagcgca ttgccgatga acgcggcatc
      181 gcgtccgagg gtcgccgcga ccacgccgcc gagcgcgccg ccgaccgcga gcatcgttcc
      241 ccacgccgcc cccaccagga cgttcgccgc cgggagctcc tccgcatcga cgaggttggg
      301 caccgctgcc gtcgccgccg gctcgaagaa cgccccgagg cccgtcatga ccccgacgag
      361 caggagcgcg aaccacaccc ccgacgcgct gtcgatcaag agcaggccca gcgccaggac
      421 cgtccgcacg agatcgacga cgatcatcag cctgcgacgg tcgaagcggt ccgcgacgta
      481 gccgccgagc ggcgagagga tcgcgaaccc gatcacctgc acgaacacga ccatcaccgt
      541 gagcgccgag gaccgcgtga tgtcgaacag gaggccgagc agggcgaccg tcgcgaacca
      601 gtcgccggcg taggagatca gctgagcccc gtagagcctg cggaaatcgc cgttgcgacg
      661 aagcagcagc agggcttccc gaggcccgct ccgctcaccc ccctccgtcg ccacgggcgg
      721 ggagcctagc agaggggtgc gtgcgatccg ccgaggaggg tacgctcctg gtcccatgtc
      781 cctcgcggcg cgcgccccgg cccagagccg tcgttctccc tggctgcgac tgctcgccgt
      841 gagcgtggcg gcgggggccg ccgcgacgta cctcgccggg acgaacgccg ggctgtgcac
      901 cccggagccc gagagcccct gcgcgaccct cgtgcgcatg ctgtcgctca gggtcggcat
      961 cgtgacgatg atcgccacgg cgctcgcact cctcacggtc gccggactcg cgcgcacgat
     1021 gcggctcgac gaggagcgga ggctggccga ggcgggggac ctgtcggact agtccttcgt
     1081 gctcattccg cccgcctcgc gtttccccca tactggaccg gacaccgatc gaaaggacgc
     1141 aacgtggcgg ttccgatccc cttcaccgac aactactccg accgctcgag cgaggccggc
     1201 taccagttcg agttcttctg cgagcggtgt ggcaacggct acaagtccgg gttccagccc
     1261 tcggccctcg ggatcggcac gaaggtcatg cgcggtctcg gtgggatgct cggcggcggc
     1321 ctcgggaacg cgagctacgc cggggaccag gtgaaggatc tgacggagtc gcaggcgaag
     1381 gacaaggctc tccagcaggc ggtccagcag gtcgcccccc tcttcaacca gtgccaccgg
     1441 tgcgggcagt gggtgtgcaa ggagatctgc tggaacgacg aagcggggct gtgcgtgaac
     1501 gatgcgccga agctcgggca ggagctcggg gcgatgcagg cccaggccca gctccagcag
     1561 gtccaggaga aggtccagca ggtggactgg acgaccgaca tgaacgtcga gcgccggcag
     1621 gtggcgcggt gcccgaactg ccaggccgag gcggagccgt cggcgaagtt ctgcgggagc
     1681 tgcggtcagg ccctcagcat gccggtgacc tgcggccgct gcgggacgga gtccccgcgc
     1741 gggacgaagt tctgtcccga gtgcggcaac cagctcggct agcgtgacgg cctaccgcgg
     1801 gcagctccgc gtcggcaacg gcgcgccggt ggccgccgcg atcgaaccga acgactccgg
     1861 cgtcgccctc tcgcccgagg gaggttcgcc gaccgagctc gcctacgtgg acatcgagga
     1921 tctgcacgac gccgactacg tcctgcggct cacggaccac accgggcaga cctacgagct
     1981 cacgatgctc gggcgcgcgt acggccaggt cctcgaggac gtccgcaagg ctcgggaggc
     2041 ccggctcgag aaggacctgc tgctccgcgg ggtcggcctg caggacacga tccccgggaa
     2101 gctcttcggg ggggagcagc ccgtcccggt tcgtctccgg ctctacgagg acctcctcgt
     2161 ggcgatcccc gagcgcggca cgatgttcgg catcccctac gcgttcgtcg aggaggtcgt
     2221 ctgggacgag gagctgtacc aggtccgggt gcgcacggat ctgggggagg agcacgtgtt
     2281 cggccacctc ggccgccggt cggaggagct gcgcgacgag ctgcgccggc tgatcgctgc
     2341 cctcgagcgc cgcaccgcgg ccaccctgcg ggcgctgctt ccggggtcgg agcccgggct
     2401 ggtgacgcgc ctggcctcga tgatgcgcga cgggcgcgtc gtgcagcgac gccgcgtgga
     2461 cgcgctcgca ccccagctgt ggggggccct cgaggacgcg gtcgcgggga cgcccgagct
     2521 gcgtgccgcg tacgacgacc tctcgtcggt cgccggttgg accgccttcg ggatcaaggc
     2581 cgtgcggccc gcggcggcgg acgggcctgc ggatgcgccg gatcccgagc cgacgggatc
     2641 gggagcgcgg gcggacctct ggtacctccg tccgctcgcc cgggaggggc ggcccctcaa
     2701 cgcggcggcg caggaggtga cctccgagcc ggggcacgcg acgtacgtct tccgcctcat
     2761 ggaaccggcc cggttcgcgt cgctgtcggg cgaggccctc gccgacgagg tcgagcggtc
     2821 gctcgccctc ctcaaccggg cgctgctcca gctgaacttc cgccgagagc ccatctacct
     2881 gagcgacgag cagatcgctg ccgacgagcg ctacgcgcgg taccgcgtcg cgctccgcaa
     2941 gctcccgcat ctccgccggg cgagggcctc cttcctcggt cgcgccgcgc acgacgccgg
     3001 gtggcggcgc cgcatcgacg agctcgcggc ctcggcctga ggttgggggg cgacgcctcc
     3061 gtctgcgaga atcaggggaa acgacgcaag gaggaccatg atgcctgggt acaccgggga
     3121 gctgtacatc ctcgccttcg accatcgcgg ctcgttccgc aagaagctcg gggtcggaag
     3181 caacccgacg ctggaggaga ccgagaaggt cgtcgacgcg aagcgcctga tcttcgaggg
     3241 cttccagcag gcgatcgcgg acggcgtgcc gcgcgagcag gcgggcctgc tcgtcgacga
     3301 ggagtacggc gccgacgtgg ctcgcaaggc ccgcgccgag ggcttcaagc tcgcgatgcc
     3361 ggtcgagaag agcgggcagg acgagttcga cttcgaatac ggctccgact acgggcagca
     3421 catcgaggag ttcgatccga ccttctcgaa ggtgctggtc cgctacaacc cggatgccga
     3481 ccccgagatg aaccggcggc agacggcgcg cctgaaggac ctctcggact ggctgcacga
     3541 gcgcgggcgg agctacctgt tcgagctgct cgttccggcg gagccggctc agctcgaggg
     3601 cgtcggcggc gacgaggacc gctacgacac ggaggttcgc cccggcctca tgctccgggc
     3661 gatcgaggag ctcctcggtg ccggggtcga gcccgacatc tggaagatcg agggcctcga
     3721 ccgtcgcgag gactgcgaga aggtctcggc gctcgtccgc tccggcggcc gcaccgaggt
     3781 cgcgtgcgtg gtgctgggcc gcggcgccga cgatgccgcg gtggaccact ggctgcgcca
     3841 gggcgcgggc gtccccggct acgtcgggtt cgcgatcggt cggtcgatct ggggcaagcc
     3901 gctctccgcg tacgtcgccg gggacctcga gcggggggac gcggcgcgcc agatcgccgc
     3961 gaactaccgc cgcttcatcg aggtctaccg gggagcgcag ggagcgacgg cgtccgcctg
     4021 agctggcggc ggcggagacg ccgcggctag gattgaccgc acccgaagag gaggcagcgc
     4081 gtggccgtca tcaagacgat cgatctcgtg ggcgtctcga ccgagtcgtg gcgcgacgcc
     4141 gcgtcccagg ccctggccga agcggcgaag acgctgcgcg gcatcgaggg gatggaggtc
     4201 ctggagacct ccgccatcgt cgagggcggc gagatcaagg agtaccagac gcacgtgcgg
     4261 atcaagttcc gcatcgagcg gtgaccgcgc ggcggggccc cagcgccccg cccgccggtc
     4321 ccccccgatg acctagactg cccgataagc cgccgacccg ggagccgcac ggagagcatg
     4381 ccagcgacga tcctcgtagg cacccagtgg ggagacgagg gcaaggggaa ggcgaccgac
     4441 cagctcgcgg accagatgga catggtggtc cgctaccagg gcgggaacaa cgcgggccac
     4501 acggtccgcg ctgcggggcg gaccctcaag ctgcacctca tcccctccgg catcctgtac
     4561 ccccacatca catccgtcat cgccgacggc gtcgtcgtgg accctcgcgt cctgctcgag
     4621 gagatggatc agctggaggc cgaggggatc ggaaccgaac gcctcatcgt ttccgcgaac
     4681 gcgcacctga tcatgccgta ccacctcgag ctcgaccgcg tgaccgagcg ctacctcggc
     4741 cggtcgaagc tggggacgac gaagcggggg atcggtccgg cgtacgcgga caaggcggca
     4801 cggatcggcc tgcgggtcca ggacctgacc gacgagaaga tcttccggca gaagctcgaa
     4861 gtcgtcctca aagagaagaa cgcgatcctc acgaaggtct acaaccgtct cccgctcgac
     4921 gcggagcgga tcgtcgagga gtacaggggc tacggcgagc gcctggcgtc gcacatcgcc
     4981 gacacctcgg ggatcctgca tgaggcgatc cgagcgggga agcgggtcct cctcgaggga
     5041 gcccagggga ccctcctcga cctcgaccac ggcacctatc cgttcgtcac gtcgtcgaac
     5101 ccggtcgccg gcggggcgct gaccggcagc ggcctcgggc cgggcgtcgt cgagcgcgtg
     5161 atcggggtga ccaaggcgta cgtgacgcgt gtgggctccg gcccgttccc gaccgaggac
     5221 gagggcgagg ccggcgcgcg gatgcaggag gtcggacagg agttcggcac cacgacgggc
     5281 cggcgccgcc gctgcgggtg gttcgacgcc gtcctgctgc gctacgcggc gcggatcaac
     5341 gggctcacgg acctgttcct gacgaagctc gacgtcctgt cgggtctccc cgaggtcaag
     5401 ctctgtgtgg cgtaccgcta cgaggggaag tcgtacgagg acctcccgcc gcaccagagc
     5461 atcatccaca aggccgagcc cgtgtacgag gcgatgccgg ggtggcccga gggcttggac
     5521 gaggtgcgga gcttcgacgc gctcccgtcg gcggcccgcg actacgtcga gcggatcgcg
     5581 gggacggccg gcgtgccggt ccgggccgtc tccgtcggac cgtcccgcga gcagacgatg
     5641 gaggtgcggt gaggtgaagg tcctcgtcgt cggaggcggc ggccgggagc acgcgctcgc
     5701 gtgggcgctg gcccggtcgg acggggtcga gcgcgtgtac gcggcgcccg gcaacgtcgg
     5761 gatcgggtcg gtcgcgacga cggttcccat cgcggcgacc gacgtgccgg cgctcgtggc
     5821 cttcgtcgag cgcgagcgga tcgatctcac ggtcatcggc ccggaggccc cgctggtggc
     5881 cgggctcgtc gacgagctcc aggcgcgggg cgcgccggcg ttcgggccga cggcggccgg
     5941 ggcccgcctc gaggggtcga aggcctgggc gcgtgacctc tgcgcccggc acgggatccc
     6001 cgcgccgtcg taccggacgt tcgacgaggc gcccgccgcg ctcgcttacc tcgactcgat
     6061 cggcgcgccg acggtggtga aggccgacgg actggcggcg gggaagggcg tcacggtggc
     6121 cgcgacgcgg gaggaggcgg cgcgtgccgt cgaggatgcg ctcgtgcgcg gccggttcgg
     6181 cgccgccggt ggccgcgtcg tgatcgagga gcacctccga ggtcgcgagg tctcggcgct
     6241 ggcgatcacc gacggccgga ccgtccttcc cctcgtgctc gcgcaggact tcaagcgcgc
     6301 gctcgacggc gacgagggac cgaacaccgg cgggatgggc gcctactcac cggtgccctt
     6361 cgtcgacccc gacaccgagg cgcgtctggt gaaggagacg ctggaggcga cggtcgcggc
     6421 cctcgcggcc gagggggtcg cctaccgcgg cgtcctctac gccggcctca tgctcaccga
     6481 ggacgggccg aaggtgctcg agttcaactg ccgcttcggt gatccggagg cacaggcggt
     6541 cctcccgcgg ctcgcgtccg acctcgcgcc cgtgctcctg gccgcggcgg gaggcggcct
     6601 cgagggcatc cgtctcgcct ggcggccgga ggcatgcgtc accgtggtcg cggcgtcggg
     6661 cgggtatccg gccgatcatc cgacgggtct gccgatcgag ggcctggatg atgccgcggc
     6721 gctggagcac gtgctcgtgt tccattccgg gacggcgcgc cgggacgata gagtggtgac
     6781 cgccggtgga cgggtcctcg cggtgagcgc gctgggcgga agcctcagcg ccgcccggga
     6841 gcgagcctac gaggcgctcg agcggatctc gttcgacggc atgcatgtcc ggaccgacat
     6901 cgccaaggag gcagcaggtg gttgaggaga acccgttggt cgggatcttg gtcggctccg
     6961 cgtcggatct gcccgtgctc cagcagggga cggcgatcct cgagcggttc ggcatcccgc
     7021 acgaaatcca tgtgatgtcc gcccaccgca accccgagca ggtcgacgag tacgttcgcg
     7081 gcgccgagga gcgcggcatc ctcgtgatga tctgcggggc cgggatggcc gcgcacctcg
     7141 cgggggccgt cgcggcgcgg accgtcctgc cggtgatcgg cgtccccgtc gggggtggag
     7201 cgatgaacgg cgtggacgcg ctgtactcga cggtgcagat gccgcgtggg gtccccgtcg
     7261 ccaccgtcgc gatcggggga gccgtgaacg cggcgatcct cgccgctcag atcatcgcga
     7321 tccgcgacga gggcatccgc aaggcgctcc acgagttcaa ggagtccctg gcggagggct
     7381 tcaagctgtg aggccaccgg ccgctcgacc ggagggccgc ggatgatccc gcgctacacc
     7441 cttccggaga tggccgccgt gtggtcggac gaggcgcgcc tgcgcaactg gctccggatc
     7501 gagctgctcg cgtgcgaagc gtgggcgagg cttgggcgga tcccggccga ggaccttcag
     7561 gcgctttcga aggcatccgt caccgcggcg cgcgtcgagg agctcgagcg ggtgacgaac
     7621 catgatgtcg cggccttcgt gcaggcggcg gcggagtccg tcggcgaggc ggggcgctgg
     7681 atccacctcg gcctcacgtc gagcgacgtc ctcgacacgg gcctcgcgct ccagctccgc
     7741 gatgcgacgg atctcctgct ccggcggctc gaagagctgc tcggcgtcgt gaaggatcgg
     7801 gcgctcgagc atcgcgacac gctctgcgtc gggcgcaccc acggggtcat cgccgagccg
     7861 accaccttcg ggtacaagct cgccgtctgg gccttcgagc tcgcgcggga ccgggag
//
LOCUS       DATLGG010000321         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_204729, whole genome shotgun sequence.
ACCESSION   DATLGG010000321 DATLGG010000000
VERSION     DATLGG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204729"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1385
                     /locus_tag="VM638_09135"
     CDS             117..1385
                     /locus_tag="VM638_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVM12619.1"
                     /translation="MRLRMRLPLLALVAVLALPGAAAADPLRDRQWGLEKVRAPEAWR
                     IARGTGVVIAVVDSGVDLRHQDLRSRLVAGWDFVDGDDRPQDEEGHGTHVAGIAAAAA
                     GNGRGIAGVAPGAKIMPVRVLDQDGTGRASDVARGIRWAVDRGADVINLSLGDLGEPL
                     FGPAFAEAIRYAWSRGAIPVVAAGNEYLLMSGYEDEPAIVVSATDRQDAKPGYSSGVG
                     AARWGLAAPGGAGGAIGSADRNILSTYWRRGERNAYAYLAGTSMAAPHVSGAAAILRS
                     AGLTPRQTVQRLLATAKDIGPQGRDQTFGHGRLDVAAATRGLARRGGTATTPAGPGPQ
                     APPPASPGTPAAAGEDPGSGGTSAAPPEPASPSEDDAAAQMPGEAGPRSQADERPQGN
                     DLMAPFVTGVVAALLAVLLGWALFRPNPPE"
     gene            1388..2221
                     /locus_tag="VM638_09140"
     CDS             1388..2221
                     /locus_tag="VM638_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HVM12620.1"
                     /translation="MRKRVVATALAKHGLNPLMRRLAGYIPGWALVETTGRRSGRPHR
                     VPVGDGLRDGAFWIVAEHGRRADYVRNLEADPAVRVRVRGRWRRGVAWILDDDDARAR
                     TRWLGGMNPVFVRVAGTDLLTVRIDLEPTEHGERTIVDVAREGAVAGLAAGIVGGIPS
                     TLHSLATGQPVLRTLAAAGNVVAPTRSPRRLLMGAGVGAHAGISAGWGIVLAATLPRR
                     RTLAWGLCAGAAIAALDLGVVGRVRPLVRGLPQLPQVADHLVYGLVAAALIRGYRRDV
                     A"
     gene            complement(2190..>2406)
                     /locus_tag="VM638_09145"
     CDS             complement(2190..>2406)
                     /locus_tag="VM638_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019360022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HVM12621.1"
                     /translation="EGSARWRLHPEPSGTRLEYEQDVRTRHRLMNLLTPIARRAFVAN
                     HRAMMRGGEAGLRRLFEVRRRRAGSHG"
BASE COUNT          279 a          872 c          933 g          322 t
ORIGIN      
        1 ggatcacccc ctgcgggatc gacgcgcgct cgtccgcgcg ctccatcttc gacctttccc
       61 ggaggggcac ctcctacacg tcggcccctg agtgcggggc gggtaggctg gccgggatga
      121 ggcttcgcat gcggctgccc ctgctcgcct tggtcgccgt actcgcgctc ccgggcgcgg
      181 cggccgccga tccgctgcgt gaccgacagt ggggcctgga gaaggtccgc gctcccgagg
      241 cgtggcgcat cgcgcggggg accggcgtcg tgatcgccgt cgtcgacagc ggggtcgacc
      301 tccggcatca ggatctgcgc tcgcgcctcg tggccggatg ggatttcgtc gacggggacg
      361 accgcccgca ggacgaggag ggccacggga cgcacgtggc cgggatcgcg gccgcggcgg
      421 ccgggaacgg acgcgggatc gccggcgtcg cgccgggcgc gaagatcatg ccggtccgcg
      481 tcctcgatca ggatggaacc ggacgtgcga gcgacgtcgc ccgcgggatc cgctgggcgg
      541 tggaccgcgg cgcggacgtg atcaacctgt ccctcggcga cctcggcgaa ccgctgttcg
      601 gtcccgcgtt cgccgaagcg atccgctacg cgtggtcgcg gggggcgatc ccggtggtcg
      661 cggctgggaa cgagtacctg ctgatgtccg gctacgagga cgagcccgcg atcgtcgtga
      721 gcgctaccga ccggcaggac gcgaagcccg ggtactcgag cggcgtcggg gccgctcgat
      781 gggggctcgc cgcccccggc ggtgccgggg gcgcgatcgg atcggccgac cggaacatcc
      841 tgtcgaccta ctggcggcgc ggcgagcgca acgcgtacgc gtacctggcg ggtacgtcga
      901 tggcagctcc tcacgtctcc ggtgccgccg cgatcctgcg ctccgcgggc ctcacgccgc
      961 ggcagacggt gcagcgtctg ctcgccacgg ccaaggacat cggcccgcag gggcgggacc
     1021 agaccttcgg ccacgggcgg ctcgacgtgg ccgcggcgac gcgaggtctc gcacgccgcg
     1081 gcggcaccgc caccactccg gcggggccag ggccgcaggc gccgccgccg gcttcacccg
     1141 ggacccccgc agcggcgggg gaggacccgg gctccggggg aacctcggcc gctccgcccg
     1201 aaccggcctc cccgtccgag gacgacgccg cggcgcagat gccgggtgag gcggggccac
     1261 gctcccaggc cgacgagcga ccgcagggga acgacctcat ggcgccgttc gtcacgggcg
     1321 tggtcgcggc cctgctcgcg gtcctcctcg ggtgggcgct gttccgcccc aacccaccgg
     1381 agtgaccgtg cgcaagcgcg tggttgcgac ggcgctcgcc aagcacggac tgaacccgct
     1441 catgcgtcgc ctcgcggggt acatccccgg gtgggccctc gtcgagacga cggggcgccg
     1501 gtccgggcgg ccgcatcggg tcccggtcgg cgacggcctg cgagacggcg ccttctggat
     1561 cgtcgccgaa cacggccgcc gcgccgacta cgtgcggaac ctcgaggcgg atccggcggt
     1621 gcgggtgcgg gttcgcgggc gctggcgccg tggcgtggcg tggatcctcg acgacgacga
     1681 cgcgcgggcc cggacgcggt ggctcggtgg gatgaacccg gtcttcgtcc gtgtcgccgg
     1741 cacggacctg ctcaccgtcc ggatcgacct cgagccgacc gagcacgggg agcgaacgat
     1801 cgtcgacgtc gcgcgcgagg gagcggtcgc gggactcgct gcggggatcg tgggcggcat
     1861 cccctcgacg ctccattcgc tcgcgaccgg acagccggtg ctccgcacgc tggccgcggc
     1921 ggggaacgtc gtcgcgccga cgcgctcccc ccgccggctc ctgatgggtg cgggggttgg
     1981 cgcgcacgcc gggatctcag ccggttgggg gatcgtcctg gcggcgacgc tcccgcgacg
     2041 gcggacgctc gcgtggggcc tctgtgcggg tgcggccatc gcggcgctcg atctcggcgt
     2101 cgtcggccgc gtgcggcccc tcgtgcgagg cctgccgcag ttgccgcagg tggcggatca
     2161 cctcgtctac gggctcgtgg ctgcggcgct catccgtggc taccggcgcg acgtcgcctg
     2221 acctcgaaca gccgcctgag ccccgcctcg ccgccgcgca tcatcgcgcg atggttggcg
     2281 acgaaggcac gacgcgcgat gggcgtcagc aggttcatga ggcggtgtcg cgttcggaca
     2341 tcctgctcgt actcgagccg ggtgccgctc ggctccggat ggagtcgcca gcgcgcggat
     2401 ccctcg
//
LOCUS       DATLGG010000322         4047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_186603, whole genome shotgun sequence.
ACCESSION   DATLGG010000322 DATLGG010000000
VERSION     DATLGG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186603"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VM638_09150"
     CDS             complement(<1..1529)
                     /locus_tag="VM638_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12622.1"
                     /translation="MRRTGWVIATLLIGSVPAAGGASEGSPATRVDAAIRRLDAGGIA
                     NAVRAAAMLAEVRPDVPRLTGAPVPAGLSSELAAPVARLLAAIDAAGAEASAAIRDPE
                     AVRERGSALSDLLVRAATSPPDPDATAELRRIERRGRASVDLPRLFGAGVLLADAIEA
                     ALPELRSVETLGSRDDAGCDILDQAPHLCIGGSGPNVYSAEAALVIDLGGNDLHTHSA
                     GAGTQARPASVTIDLGGHDRYERSTGPAQGAGNLGIGMLVDTGGNDTYAIAATAGQES
                     AIGQGTNFSAGGGMLVDLGGRDRYLLTSTKPDQAFAAGQGRAAQPGFGILLDDGADED
                     EYVLSARPSAVIEGDDEVKLGGLNANGMAFGALGGVGLIRDGGGSDFMTLETVSATID
                     PHETRPVTSVPLTGPQGLGGSSNGVGLLRAGVGSTNRRAIGSMQAPWSGIQTVNAFGW
                     SVGGLGVLRDEGGDDIYRAEAVSHASRTIALDDACGCDGASAEAKQSIPGVGAASVRG
                     MG"
     gene            complement(1531..3903)
                     /locus_tag="VM638_09155"
     CDS             complement(1531..3903)
                     /locus_tag="VM638_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HVM12623.1"
                     /translation="MSRVRTFVLAAVVAVLALPSLAGASAPGLPDIPGPRVPVATPDE
                     PEQPALYRFVVPHRETIDELNDRGFDLAERVAPVEGGFEVDIIATPEELTRLAFMGLR
                     PVRVLETVETWRRRVAEREAQIAAEERVVQELDDVKILRADYFVARNGTKYLSVEAKH
                     AAGIIATDQLVLLYDRGPGTPVGSGGAPVAMSRFVDSGVYLYHRTQVAVSERPSEVMV
                     VSALGGQATRQVRDWLNAPPPDPKGDYLWDFISRAMDPTEVYQRIETLAAEYPGIAEI
                     VELPYKTHGYRRPARATLGTTDASRVVVASKAYGHEGGNDIRVSFVNPGAPNHPLTVK
                     TLGSEVTVELGTNAAGTASSTAAQVAAALNEQAAALVSAHTYRGNAGGGVVAPASAQL
                     SDNLKAPARISREPVTVKVLRIRGDRENVKTGVFLYAQEHAREWVTPLVAVETAERLV
                     RNYDTDPATKRLVDDLDIFILPSVNPDGGHYSFYDFNMQRRNMSNHCGPENSDPGRQN
                     AWGVDLNRNFTVGSRFDGYSGASSSCTSDTYSGPAELSEAEAKNEVWLTERFPNIRFA
                     MNTHSYGDYFMWAPGAYVASGRIELPRPQLGHDHFFWESAEHILNGVKAHRGTVVTPA
                     RTGPVSEVLYSAAGNSADEHYYNKGIFGWSFEAGNLGFQPAFADLHEEAMEYANGWIS
                     ILEVARKFELDRQAPTSELVTSVDVATGDRYVRFRTNEPADVYYTTDGSRPTFASTRY
                     KAAGLREGPEVLTFSGPVTIKWFAADVKGQVEGGYDPSGDGAGYRQQTVE"
BASE COUNT          548 a         1489 c         1408 g          602 t
ORIGIN      
        1 cccatcccgc ggacgctcgc cgcaccgacg ccggggatcg actgcttcgc ctcagcggaa
       61 gcgccgtcgc acccgcacgc gtcgtcgagg gcgatcgtgc gcgacgcgtg cgagaccgct
      121 tccgcgcgat agatgtcgtc gccgccctcg tcccgcagga caccgaggcc gccgacgctc
      181 cacccgaacg cgttgacggt ctggatcccg ctccacggcg cctgcatcga cccgatcgcg
      241 cgccggttcg tcgaccccac cccggcacgc aggagtccga caccgttcga agagccaccg
      301 agcccctgcg gacccgtcag cggcaccgac gtcaccgggc gcgtctcatg cgggtcgatc
      361 gtcgccgaca cggtctcgag cgtcatgaaa tcgctgccgc cgccatcgcg gatcagcccc
      421 accccgccga gggcgccgaa cgccatgccg ttcgcgttca gccccccgag cttcacttcg
      481 tcgtcgccct cgatcacggc cgacggacgc gcgctcagca cgtactcgtc ctcgtccgct
      541 ccgtcgtcga gcaggatccc gaaccccggc tgcgccgcac ggccctgtcc ggccgcgaac
      601 gcctgatccg gcttcgtgct cgtgagcagg tatcggtcgc ggcccccgag gtcgaccagc
      661 atccccccgc cggccgagaa gttggtcccc tgtccgatcg ccgactcctg cccggcggtg
      721 gcggcgatcg catacgtgtc gttgccgccg gtgtcgacga gcatcccgat gccgaggttg
      781 ccggcgccct gcgcggggcc cgtcgaacgc tcgtagcggt cgtggccgcc gaggtcgatc
      841 gtcaccgatg ccggccgggc ctgcgtgccc gcaccggcac tgtgggtgtg cagatcgttc
      901 ccgccgagat cgatcacgag cgccgcctcg gcgctgtaga cgttgggacc gctcccgccg
      961 atgcagaggt gcggcgcctg gtcgaggatg tcgcagccgg cgtcgtcccg cgacccgagc
     1021 gtctcgaccg agcgcagctc cggcaaagca gcctcgatcg cgtcggcgag gaggaccccg
     1081 gccccgaaga ggcgaggcag gtcgacgctc gcgcgcccgc gccgttcgat ccgccgaagc
     1141 tcggcggtcg cgtcggggtc gggcggagag gttgccgcgc gcacgagcag atctgagaga
     1201 gcggagccgc gctcgcgcac cgcctccgga tcccggatcg ccgccgacgc ctcggcgccc
     1261 gcggcatcga tggcggcgag cagccgcgcg accggtgccg cgagctcgct gctcagcccc
     1321 gccgggaccg gagcccccgt gagccgcggc acatcaggcc ggacctccgc gagcatcgcg
     1381 gcggcgcgca ccgcgttcgc gatcccgccg gcgtcgagac gacggatcgc ggcgtcaacg
     1441 cgcgtggccg gcgagccctc ggaggcgccc cccgcggccg gaacgctccc gatgaggagc
     1501 gtcgcgatga cccaacccgt tcgccgcacg ctactccacc gtctgctgcc ggtagccggc
     1561 gccgtcgccc gacgggtcgt agccgccctc gacctgtccc ttcacgtccg cggcgaacca
     1621 cttgatcgtg acggggccgc tgaaggtcag cacctccggc ccctcgcgga gtccggccgc
     1681 cttgtagcgg gtcgacgcga aggtcggccg gctcccgtcg gtcgtgtagt agacgtcggc
     1741 cggctcgttc gtgcggaagc gcacgtagcg gtcgcccgtt gcgacatcga cgctcgtgac
     1801 gagctcggag gtgggcgcct gccggtcgag ctcgaacttc cgagcgacct cgaggatcga
     1861 gatccaaccg ttcgcgtact ccatcgcctc ttcgtgcagg tccgcgaagg cgggctggaa
     1921 gccgaggttc cccgcttcga agctccagcc gaagatcccc ttgttgtagt agtgctcgtc
     1981 cgcggagttc ccggccgccg agtacagcac ctcgctcacg gggcccgtcc gagcgggcgt
     2041 cacgaccgtt ccccggtgcg ccttgacgcc gttgaggatg tgctccgccg actcccagaa
     2101 gaagtggtcg tgaccgagct gcggacgcgg caactcgatc cgcccgctcg cgacgtacgc
     2161 gcccggtgcc cacatgaagt agtcgccgta gctgtgcgtg ttcatcgcga accggatgtt
     2221 cgggaagcgc tccgtcagcc acacctcgtt cttcgcctcg gcctcgctca gctcggcggg
     2281 tccggagtag gtgtcgctcg tgcagctcga cgaggcgccg gagtacccgt cgaagcgcga
     2341 cccgaccgtg aagttccggt tcaggtccac gccccaggcg ttctgccgac cggggtcgga
     2401 gttctccggc ccgcagtggt tcgacatgtt ccggcgctgc atgttgaagt cgtagaagga
     2461 gtagtggccg ccgtcggggt tcaccgacgg caggatgaag atgtcgaggt cgtcgacgag
     2521 gcgcttcgtc gcgggatcgg tgtcgtagtt gcgaaccagc cgctccgccg tctcgaccgc
     2581 aacgagcggg gtcacccact cgcgcgcgtg ctcctgcgcg tagaggaaga cgcccgtctt
     2641 cacgttctcg cggtcgccgc ggatccgcag caccttcacc gtgaccggct cgcgcgagat
     2701 ccgcgcgggc gccttgaggt tgtcgctgag ctgagcggat gcgggcgcga cgaccccacc
     2761 gccggcgttc ccgcggtagg tgtgcgcgga gacgagggca gccgcctgct cgttcagcgc
     2821 ggcggccacc tgcgcggcgg tgctgctcgc cgtgcccgcg gcgttcgtcc ccagctcgac
     2881 ggtgacctcc gagcccaggg tcttcacggt cagggggtgg ttcggcgcgc ccgggttcac
     2941 gaagctcacc cggatgtcgt tgccaccctc gtgcccgtag gccttcgacg ccacgacgac
     3001 ccgtgacgca tcggtcgtgc cgagcgtcgc gcgcgcgggc cggcggtagc cgtgggtctt
     3061 gtacgggagc tcgacgatct cggcgatccc ggggtactcg gcggcgagcg tctcgatccg
     3121 ctggtagacc tccgtcgggt ccatcgcgcg cgagatgaag tcccagagat agtcgccctt
     3181 cgggtcgggg ggcggcgcgt tcagccagtc ccggacctgg cgcgtcgcct gcccgccgag
     3241 cgcgctcacg accatgacct cggacggacg ctcgctcacc gcaacctgcg tgcggtggta
     3301 gaggtagacc ccggagtcga cgaaccggct catcgcgacg ggtgcgcctc ccgaaccgac
     3361 cggggtgccg gggccgcggt cgtagaggag cacgagctgg tcggtcgcga tgatgcctgc
     3421 cgcgtgcttc gcctcgacgg agaggtactt cgtgccgttg cgcgcgacga agtagtcggc
     3481 gcggaggatc ttcacgtcgt cgagctcctg gacgacgcgc tcctcggcgg cgatctgggc
     3541 ttcgcgctcc gcgacccgcc ggcgccaggt ctcgaccgtc tcgagcaccc ggaccgggcg
     3601 cagccccatg aacgcgagcc gcgtcagctc ctcgggcgtc gcgatgatgt cgacctcgaa
     3661 cccgccctcg accggggcga cccgctcggc caggtcgaac ccgcggtcgt tcagctcgtc
     3721 gatcgtctcg cgatgcggga cgacgaaccg gtagagcgcc ggctgctcgg gctcgtcggg
     3781 ggtggcgacc ggcacgcgcg ggccggggat gtccggcagg cccggggcgg atgcacccgc
     3841 gagcgacggc agcgccagga ccgcgacgac ggcggcgagg acgaaggttc ggacgcgcga
     3901 catggaactc cctcccgatc ggttctccag gtgttcggcg tgcctctgcc cgaacccttc
     3961 gtgcacctgc acaaagggtc cgccacacac ggtcggcggc cggtttggtc acctgcacaa
     4021 aggaggcacg gccccagagc gcgaatc
//
LOCUS       DATLGG010000323         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_236520, whole genome shotgun sequence.
ACCESSION   DATLGG010000323 DATLGG010000000
VERSION     DATLGG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236520"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..504)
                     /locus_tag="VM638_09160"
     CDS             complement(178..504)
                     /locus_tag="VM638_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVM12624.1"
                     /translation="MGGGHRCRRCEPLPDEVRRAADLLGRRWLLAILYASHSGAVRFN
                     EFRQVVGPIPPRTLAQRLAELEEAGVLERIVHEDERPPRVEYRLTDAGRRLKALVQAL
                     RAWPAA"
     gene            complement(504..>2361)
                     /gene="lon"
                     /locus_tag="VM638_09165"
     CDS             complement(504..>2361)
                     /gene="lon"
                     /locus_tag="VM638_09165"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HVM12625.1"
                     /translation="ADTMGYAPEVPLAAKLDILGTPDVVERLRKAVAAQRERLADAGL
                     RRRIREDVAEGMERSQREMLLRRQLEAIRRELGEDGDGDDDWATRIEAAGMPEAARRE
                     AERELARLERQPEGAEAGMIRTYLEWMVSLPWSARSEERLDVPAAREVLDADHAGLER
                     VKERIVEYLAVRKLRRERDLDEGRSGVILTLVGPPGTGKTSLGHSIARALGREFARIS
                     LGGIRDEAEIRGHRRTYVGALPGRLVRALREAGTLNPVIMLDEVDKVGADWRGDPSSA
                     LLEVLDPAQNHSFRDHYLDVELDLSEVLFIATANVADTIPAPLLDRMEVIRFDGYTVD
                     EKLAIARGYLWPRQRERNGLHPDEVSVSDDVLETVVREYTREAGVRNLERELGTILRK
                     TATRIASGQLEAPVAVDLALVRDALGRQRFFQEAAERTAVPGVATGLAVTGTGGDVLF
                     VEATAMSGDDGLVLTGQLGDVMKESARIALSWVRAHAAELGIAEDDLRGRSFHVHVPA
                     GAIPKDGPSAGITIATALASLLSDRPVRHTVGMTGEVTLQGRVLPIGGVKQKVLAAHA
                     AGLTHVVLPERNRADLDDVPADVREQLTFHPVMSLGEVLELALEPAREQLAA"
BASE COUNT          277 a          941 c          842 g          301 t
ORIGIN      
        1 cccaacatgg ccgcggcggc catggccgcg cgccgaaagc cgcttcgcga gcgggatcgc
       61 gcccacctgt ccctcgcgga cgcggcgccg ccggccacgt ccggggtcag acccccgacg
      121 tggccgctgg ggacggtcct cggcactcgc ggggcgcgag tgccgccgcc gctccggtta
      181 cgcggccggc caggcgcgga gcgcctggac gagcgccttc agccgccggc ccgcgtcggt
      241 gaggcggtac tcgacgcgcg gcggccgctc gtcctcgtgg acgatccgct ccagcacccc
      301 cgcctcctcg agctcggcga ggcgctgcgc gagcgtccgc ggcgggatcg gccccaccac
      361 ctgccggaac tcgttgaacc gcaccgcgcc ggagtgcgac gcgtagagga tcgcgaggag
      421 ccaccgccgc ccgagcaggt cggccgcgcg ccgcacctcg tcgggcagcg gctcgcagcg
      481 ccggcagcgg tggcccccgc ccatcaggcc gcgagctgct cgcgcgccgg ctcgagcgcg
      541 agctcgagca cctcgccgag gctcatcacc gggtggaacg tcagctgctc gcgcacgtcc
      601 gccgggacgt cgtcgaggtc ggcccggttc cgctcgggga ggacgacgtg cgtcagcccg
      661 gccgcgtgcg ccgcgagcac cttctgcttg acgccgccga tcggcagcac gcgcccctgc
      721 agcgtcacct cgcccgtcat gccgacggtg tggcgcaccg gccggtcgga gagcagcgac
      781 gcgagcgccg tcgcgatcgt gatccccgcg ctggggccgt ccttgggaat cgcgcccgcg
      841 gggacgtgca cgtggaacga gcgcccgcgc agatcgtcct ccgcgatccc gagctcggcc
      901 gcgtgcgcgc gaacccacga gagcgcgatc cgcgccgact ccttcatcac gtcgccgagc
      961 tgcccggtca ggacgaggcc gtcgtcgccg ctcatcgccg tcgcctcgac gaagaggacg
     1021 tctccgcccg tgcccgtcac cgcgaggccc gtcgccacac ccggcaccgc cgtccgctcc
     1081 gccgcctcct ggaagaaccg ctgccggccg agcgcgtcgc ggacgagcgc gagatcgacc
     1141 gcgacgggcg cctcgagctg ccccgacgcg atccgcgtcg ccgtcttccg caggatcgtc
     1201 cccagctcgc gctcgaggtt ccgcaccccc gcctcgcgcg tgtactcgcg cacgaccgtc
     1261 tccagcacgt cgtcggacac ggagacctcg tcggggtgca ggccgttccg ctcgcgctgg
     1321 cgcggccaca ggtatccgcg cgcgatcgcg agcttctcgt cgaccgtgta cccgtcgaag
     1381 cggatcacct ccatccggtc gagcagcggc gccgggatcg tgtcggcgac gttcgcggtc
     1441 gcgatgaaga gcacctcgga caggtcgagc tcgacgtcga ggtagtggtc gcggaacgag
     1501 tggttctggg ccgggtcgag gacctccagc agggccgagg aggggtcgcc gcgccagtcg
     1561 gcgccgacct tgtcgacctc gtccagcatg atcaccgggt tcagcgtgcc ggcctcgcgc
     1621 agcgcgcgca cgagccgccc cggaagcgcg ccgacgtagg tgcgccggtg cccgcggatc
     1681 tcggcctcgt cgcggatgcc gcccaggctg atgcgcgcga actcccgccc cagggcccgg
     1741 gcgatcgagt ggcccagcga ggtcttgccc gtgccgggcg gcccgaccag ggtcaggatc
     1801 acgccgctgc gcccctcgtc caggtcgcgc tcgcgccgca gcttgcgcac cgcgaggtac
     1861 tcgacgatgc gctccttcac ccgctccagc cccgcgtggt cggcgtcgag cacctcccgc
     1921 gcggccggga cgtcgagccg ctcctcggag cgcgcgctcc acggcagcga caccatccac
     1981 tccaggtagg tgcggatcat ccccgcctcg gcgccctcgg gctggcgctc caggcgcgcg
     2041 agctcgcgct cggcctccct gcgggcggcc tcgggcatgc cggcggcctc gatgcgggtg
     2101 gcccagtcgt cgtcgccgtc cccgtcctcg cccagctcgc ggcggatcgc ctccagctgg
     2161 cggcgcagga gcatctcgcg ctggctgcgc tccatgccct cggcgacgtc ctcccggatg
     2221 cgccggcgca ggccggcgtc ggccagccgc tcgcgctggg cggcgacggc cttccgcagg
     2281 cgctcgacca cgtcgggggt gcccaggatg tcgagcttcg cggccagcgg gacctcgggg
     2341 gcgtagccca tagtgtcggc c
//
LOCUS       DATLGG010000324         9735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_205419, whole genome shotgun sequence.
ACCESSION   DATLGG010000324 DATLGG010000000
VERSION     DATLGG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205419"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VM638_09170"
     CDS             <1..363
                     /locus_tag="VM638_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVM12626.1"
                     /translation="EDFYRSYGTLVALGIGGAALVAVAVLLARVVAPRRQLPEKLTTY
                     ECGMDPVGTGWSQTQVRYYIYAFLFVIFDVESVFLFPWAVVFEQLGWFAVVEMTIFIL
                     ILGGGLLYAWRKGVLRWV"
     gene            363..857
                     /locus_tag="VM638_09175"
     CDS             363..857
                     /locus_tag="VM638_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HVM12627.1"
                     /translation="MENVPMPQPVKFLLNWGRRYSLWVMNFGLACCAIELIATSTSKH
                     DFIRLGVIPFAHGPRQADLLVVAGTVTDKMAPALKRVYDQIPDPKYVISFGSCSNCGG
                     PYWDSYSVTKGVDQIIPSDVYVPGCPPRPEALLHGIVKLQEKIANEDIREKWTGKLTT
                     PTTS"
     gene            854..1402
                     /locus_tag="VM638_09180"
     CDS             854..1402
                     /locus_tag="VM638_09180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVM12628.1"
                     /translation="MSRTERETRAAAEPLPGAEIAERLRARFGEDVPEAEEIHGQAVV
                     RVTPERYRDLILTLRDDDELAFDFYDFTSAVDWEDAGFDVVTQLYSTTRRHHVRVKVR
                     CSAVDPVCPTIHDLYPGADWYERETWELFGIRFDGHPRLVKLLLPQEFEGYPLRKSFP
                     LMSRVVKQWPGAAEGADEEDDD"
     gene            1402..2412
                     /gene="nuoH"
                     /locus_tag="VM638_09185"
     CDS             1402..2412
                     /gene="nuoH"
                     /locus_tag="VM638_09185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVM12629.1"
                     /translation="MLAQAGVSVIDQLVNNVWFAIAIKTLLVMGFFLTAPMVVGYTEH
                     KVLAHMQHRLGPMEAGRFHGWAQLIADGVKFIQKEDVIPRAADRWIFTLAPAVFLVPG
                     VMTLLVVPLGDTWFAENLDVGLFFIMAISAVPVIGVLMAGWASANKFSLIGALRGAAQ
                     LIAYELPLILAAVAVAMLAGTLSLVGIVESQQAFWFVLFQPLGFFIFMTASLAELVRT
                     PFDMPIADSEIIFGAYTEYTGMKFAFFLLAEYAGIVILSAVAAVLFLGGYLAPPGLEF
                     IPGPIWMAAKIGALSFLIIWLRATYPRLREDQLQRFSWIVLIPLSLANILVTAVVKVW
                     IG"
     gene            2412..3791
                     /locus_tag="VM638_09190"
     CDS             2412..3791
                     /locus_tag="VM638_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HVM12630.1"
                     /translation="MAIDTSTRVPQIVDEPDEVFRPGSPPGQITVDGESYRRRSPLGV
                     WLLSLLTLGLYAMVWHYKVNDEARRYLRDTRIVPGFAFLAFSLAGLALVPAALYWLFA
                     DRLTFWGFALWIAAGLPFLIPPIITVYRTGDRIRRMQEQAGLEPTANGLVGFLLMPAL
                     LAAVPYYQAELNLIWGRTPLLPGIGLLKGLFVTLRHLLSRSVTQQYPKEKPVLPPRTR
                     GVISLKAENCTVCWKCSRECPDWCIYIESHKETHEPASGGRARSAKVLDRFAIDYALC
                     MYCGICVEVCPFDALFWSPEFEYATYGIHDLVHEKEKLDEWNYTVLPPPELEEGADPV
                     EDAPPEGGAPHSEARTSATPVAKPGIAEASGGPPAAPPAQAAVEAPPQERPGGPAPAE
                     KLPESPAPGEQTKPPHDRPAHVEPDAETYDRVLAEELGKGTDRRVAEGRAKSAAVREA
                     RRKQTEGGG"
     gene            3788..4282
                     /locus_tag="VM638_09195"
     CDS             3788..4282
                     /locus_tag="VM638_09195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVM12631.1"
                     /translation="MTGQEWTFALLAVVGGIGAVRLVTSKNVIHAALYLVVALSAVGG
                     VYLLLGSEFVAWTQILIYVGAIVVLLLFSLMLTRAPIGREALDNQQRGLAAVVSLAIL
                     AGLGLLIVEGFGGQEIRTEAPSLTGEIGIRMFSDFVLPFEVVSFLLLAALVGAIMLAR
                     KDEA"
     gene            4279..4587
                     /gene="nuoK"
                     /locus_tag="VM638_09200"
     CDS             4279..4587
                     /gene="nuoK"
                     /locus_tag="VM638_09200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVM12632.1"
                     /translation="MIRLPLVLLFSAFLFSAGVYGVLARRNAVLVLMSIELMLNAVNV
                     NLVAFSRHLNDALLAGEIFTLFVITVAAAEVGIGLAIVILIFRNRETVNVDEVDLLRW
                     "
     gene            4581..6512
                     /gene="nuoL"
                     /locus_tag="VM638_09205"
     CDS             4581..6512
                     /gene="nuoL"
                     /locus_tag="VM638_09205"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVM12633.1"
                     /translation="MVELAWLIPVIPLSAALLIALFGRRLPGRGAELGILATGVSFLL
                     SLAVAGAVIGDHEFHLERSLTWFRFGSFELELGMNIDGLAAMMFVVVTTVSLLVQIYS
                     TGYMKGEPRFTWYFAALNMFTGSMLTLVIANNLFQLLVGWELVGVCSYLLIGHFWEEK
                     PNQDAAMKAFITTRTGDVGFLFGIFVLTFAAGSANIGHISEMAEHGEIAAGVLTAGAL
                     LLFCGAIGKSAQFPLHVWLPDAMAGPTPVSALIHAATMVVAGIYLIARMFPVFIHAGE
                     VALQFVSIIAGITMFIAALLAVIQDDIKRVLAYSTISQLAYMVAGLSLGEHGYTPGLF
                     HLFTHAFFKALLFLGAGSVIHAVHSNNMSDMGGLRRSMPVTFWTFLIGSAALAGIPPL
                     AGFWSKDEIITTAFVEGHYVLWAVAFATAILTAFYMTRAVLLTFFGDYRGHGHPHESP
                     RVMTVPLVILAVLSVSVGFLNATPFGIHLFADWVFFHEPHHAAFNYGFAAIAIVGAAL
                     GIGVGYRLYAGWRDRDPVRKLGPLYRFLDRKYLLDDIYLRGIVRPIQYPIARAVNWTN
                     RYILDGIVHLVAWVTRKLGGVTAAVDRNLVDGAVNRVAIGTGWTGGLLRYVQSGNVQR
                     YAVLLFTGVVILTVVFTRV"
     gene            6523..8061
                     /locus_tag="VM638_09210"
     CDS             6523..8061
                     /locus_tag="VM638_09210"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVM12634.1"
                     /translation="MDWFDDWAITLAVFIPLVGALFVSLLPRRADRLIRAMGILFTAV
                     PLVIGIAMLFRFDFGAPGAFGAARAADALQFHVDVSWIQAIGARYHIGLDGISLPLFE
                     LTLLLSFLCMIYTWKFLPEPGRPKAFIALLLLLETGMAGSFIAFDLILFFVFWELVLV
                     PMYFLIGVWGGPNREYAAIKFFLYTLFGSIFMLLGFLAMYFRADPNTFDMIALQSASF
                     PHAFQVVVFGGLFLGLAIKVPMWPFHTWLPDAHTEAPTVGSVILAGILLKMGTYGFVR
                     IALPILPDGAKVWAPWIGILSVIAIIYAALACLAQRDMKRLIAFSSVGHMGFVMLGIA
                     TLTDIGINAAIFGMVAHGIITGMLFFLAGSVHERYHTREFGEIGGGMLKKVPYLGSAF
                     AYTAIASLGLPGLAGFWGEVMALAGSFQPAEGLSVGLFRTLMVLGGIGTILTAGYMLW
                     MIQRVNLGTVPDRWEGKAFSDITAVEYVAWTPLLIGILLLGIVPALLFGMTSPAVGSL
                     TGMF"
     gene            8062..9522
                     /locus_tag="VM638_09215"
     CDS             8062..9522
                     /locus_tag="VM638_09215"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HVM12635.1"
                     /translation="MLPQIDYRAIAPELILAGTIMVVLVVDLFLRERRKHWSMTVSFV
                     GVATALGSTIPLFGREGPAFGETYVVDPFAVLLKIFFLAMALGVLFLSQRYFREGRFY
                     QGEYYFLLLTSFLGMLTMASSRDLLMLFISLELVSAPGFIIAGLRKRDPRSNEAALKF
                     FLIGILAVAVMLYGMSLLYGVTGTIALEGIARSLQQGAEPLAIAALLFVVVGFAFKIS
                     AVPFHFWAPDTYEGSPVPVAAYLSTASKAAGFAGLLQIMFVAFRPVADVWAPVFAVLS
                     ILTMTLGNLIAIQQRQVVRLLAYSSIAQAGYMLLPFALVGLSPDLDQRAFGAAVLYIL
                     IYGATNLGAFGVVTSVSRESPGVLVEDFAALGQRAPGLSVAMTTFMISLAGIPPTAGF
                     IGKLFIFLVAISAWQAGSTIALVLAVAMVINSVISLYYYAAVVRQMFLVPIERPGRFR
                     SPVLVTGVVAASLVVVLAVGIFPDLFASFPPDATLP"
BASE COUNT         1277 a         3463 c         3176 g         1819 t
ORIGIN      
        1 gaagacttct atcgctcgta cgggacgctg gtcgccctcg gcatcggggg agcggccctg
       61 gtcgcggtcg ccgtcctgct cgcccgggtc gtggccccgc ggcgccagct ccccgagaag
      121 ctgaccacct acgagtgcgg catggacccg gtcgggacgg gctggtcgca gacccaggtc
      181 cgctactaca tctacgcctt cctgttcgtg atcttcgacg tcgagagcgt gttcctgttc
      241 ccgtgggcgg tcgtgttcga gcagctcggg tggttcgccg tcgtcgagat gacgatcttc
      301 atcctgatcc tgggcggcgg cctcctctac gcgtggcgga agggggtgct gcggtgggtc
      361 tgatggagaa cgtcccgatg ccgcagccgg tgaagttcct gctgaactgg ggccgccgct
      421 actcgctgtg ggtcatgaac ttcggcctcg cctgctgcgc gatcgagctg atcgcgacct
      481 cgacctcgaa gcacgacttc atccgcctcg gtgtgatccc gttcgcgcac ggcccgcgcc
      541 aggccgacct gctcgtggtc gccgggaccg tcaccgacaa gatggcgccg gcgctcaagc
      601 gcgtctacga ccagatcccc gacccgaagt acgtgatctc gttcggttcg tgctccaact
      661 gcggcggccc gtactgggac tcctactcgg tgacgaaggg cgtggaccag atcatcccgt
      721 ccgacgtgta cgtgccgggc tgcccgccgc gccccgaggc cctcctccac ggcatcgtca
      781 agctgcagga gaagatcgcg aacgaggaca tccgcgagaa gtggaccggc aagctgacga
      841 cccccacgac gtcgtgagcc ggaccgaacg cgagacgcgc gctgccgccg agccgcttcc
      901 gggcgccgag atcgccgagc gcctgcgcgc gcggttcggc gaggacgtcc ccgaggcgga
      961 ggagatccac ggccaggccg tcgtgcgcgt cacccccgag cggtaccggg acctcatcct
     1021 caccctgcgc gacgacgacg agctcgcctt cgacttctac gacttcacga gcgccgttga
     1081 ctgggaggat gccggcttcg acgtcgtcac ccagctgtac tcgacgaccc gtcggcacca
     1141 cgtccgcgtg aaggttcgct gctcggcggt cgaccccgtg tgcccgacga tccacgacct
     1201 ctacccgggg gccgactggt acgagcggga gacgtgggag ctgttcggca tccggttcga
     1261 cgggcacccg cggctcgtga agctcctcct gccgcaggag ttcgaggggt accccctgcg
     1321 gaagtcgttc ccgctgatga gccgggtcgt caagcagtgg cccggcgccg cggagggcgc
     1381 cgacgaggag gacgacgact gatgctcgcc caggccggcg tcagcgtgat cgaccagctc
     1441 gtcaacaacg tgtggttcgc gatcgcgatc aagaccctgc tcgtgatggg gttcttcctc
     1501 accgcgccga tggtcgtggg ctacacggag cacaaggtgc tcgcccacat gcagcaccgg
     1561 ctcgggccga tggaggccgg acggttccac ggctgggcgc agctgatcgc cgacggcgtg
     1621 aagttcatcc agaaggagga cgtgatcccg cgcgcggcgg accgctggat cttcacgctg
     1681 gcgccggcgg tcttcctcgt gcccggggtc atgaccctgc tcgtcgtccc gctcggcgac
     1741 acgtggttcg ccgagaacct cgacgtcggc ctcttcttca tcatggccat ctcggccgtc
     1801 cccgtgatcg gcgtgctgat ggcggggtgg gcctcggcca acaagttctc gctgatcggc
     1861 gccctgcgcg gcgcggccca gctgatcgcc tacgagctcc cgctgatcct cgccgccgtc
     1921 gccgtcgcga tgctcgccgg caccctgtcg ctggtcggca tcgtcgagtc ccagcaggcc
     1981 ttctggttcg tgctcttcca accgctcggg ttcttcatct tcatgacggc gtccctcgcg
     2041 gagctcgtgc gcacgccctt cgacatgccg atcgccgact ccgagatcat cttcggcgcg
     2101 tacaccgagt acaccggcat gaagttcgct ttcttcctgc tggccgagta cgcgggcatc
     2161 gtgatcctgt cggcggtcgc ggcggtcctg ttcctcggtg ggtacctcgc tccgccgggg
     2221 ctcgagttca tcccgggccc gatctggatg gcggcgaaga tcggggcgct ctccttcctc
     2281 atcatctggc tgcgcgcgac gtacccgcgt ctgcgcgagg accagctcca gcgcttctcc
     2341 tggatcgtgc tcatcccgct ctccctggcg aacatcctcg tcacggccgt cgtgaaggtc
     2401 tggatcggct gatggcgatc gacacgagca cccgggtccc ccagatcgtc gacgagccgg
     2461 acgaagtctt ccgccccggc tcgccgcccg ggcagatcac ggtcgacggc gagtcgtacc
     2521 gtcgccgcag cccgctcggt gtctggctgc tgtcgctcct cacgctcggc ctctacgcga
     2581 tggtctggca ctacaaggtc aacgacgagg cgcgccggta cctgcgcgac acgaggatcg
     2641 tcccgggctt cgcgttcctc gcgttctcgc tcgcggggct ggcgctcgtc ccggcggcgc
     2701 tctactggct cttcgccgac cgcctgacgt tctgggggtt cgccctgtgg atcgcggcgg
     2761 gcctcccgtt cctgatcccg ccgataatca cggtctaccg gacgggggac cgcatccggc
     2821 ggatgcagga gcaggccggt ctggagccga cggcgaacgg cctcgtcggc ttcctgctca
     2881 tgccggcgct cctggcggcg gtgccctact accaggccga gctcaacctg atctggggga
     2941 ggaccccgct gctcccgggg atcgggctcc tcaagggcct gttcgtcacg ctccgccacc
     3001 tgctgtcacg gtcggtcacc cagcagtacc cgaaggagaa acccgtcctg ccgccccgca
     3061 cgcggggcgt gatctccctc aaggccgaga actgcacggt ctgctggaag tgctcccgcg
     3121 agtgcccgga ctggtgcatc tacatcgagt cgcacaagga gacgcacgag cccgcgtccg
     3181 gcgggcgtgc gcgctcagcg aaggtgctcg accgcttcgc gatcgactac gcgctctgca
     3241 tgtactgcgg gatctgcgtc gaggtctgcc cgttcgacgc gctgttctgg agcccggagt
     3301 tcgagtacgc gacgtacggg atccacgacc tggtccacga gaaggagaag ctcgacgagt
     3361 ggaactacac cgtgctcccg ccgcccgagc tcgaggaggg agccgacccg gtcgaggacg
     3421 caccgccgga gggtggcgct cctcattcgg aggcgcgcac gtccgccacg ccggtcgcaa
     3481 agccgggcat cgcggaggcg agcggagggc cccccgcggc acctccggcg caggcagcgg
     3541 tcgaggcccc gccgcaggag cgtcccgggg ggcccgcccc cgccgagaag ttgccggagt
     3601 cgcccgcgcc gggggagcag acgaagccgc cgcacgaccg gcccgcgcac gtcgagcccg
     3661 acgccgagac ctacgaccgg gtcctcgccg aggagctcgg gaagggcacg gaccgccgcg
     3721 tcgcggaagg ccgggcgaag tcggccgcgg tccgcgaggc ccgcaggaag cagaccgagg
     3781 gcggcggatg accggccagg agtggacctt cgccctcctg gcggtcgtcg gcgggatcgg
     3841 cgccgtgcgc ctcgtcacct cgaagaacgt gatccacgcg gccctctacc tcgtcgtcgc
     3901 gctctccgcc gtcgggggcg tctacctcct gctggggtcc gagttcgtcg cgtggaccca
     3961 gatcctgatc tacgtcggcg cgatcgtcgt gctcctgctg ttcagcctga tgctcacgcg
     4021 ggcgccgatc ggccgggagg cgctcgacaa ccagcagcgc ggcctcgcgg cggtcgtctc
     4081 gctcgcgatc ctcgccggcc tcgggctgct gatcgtcgag ggcttcgggg gccaggagat
     4141 ccggaccgag gcgccgtcgc tcaccggcga gatcgggatc cgcatgttca gcgacttcgt
     4201 cctgccgttc gaggtggtgt cgttcctcct gctggcggcg ctggtcgggg cgatcatgct
     4261 cgcgcggaag gacgaggcgt gatccgcctg cccctcgtcc tcctgttctc ggccttcctc
     4321 ttctccgccg gcgtctacgg agtgctcgca cggcgcaacg cggtgctcgt cctcatgtcg
     4381 atcgagctga tgctgaacgc cgtcaacgtc aacctcgtgg ccttctcgcg gcacctgaac
     4441 gacgcgctcc tcgccggcga gatcttcacg ctgttcgtga tcaccgtggc cgccgccgag
     4501 gtcgggatcg gcctggcgat cgtgatcctg atcttccgca accgcgagac cgtcaacgtc
     4561 gacgaggtgg acctgctgcg atggtagagc tcgcctggct gatccccgtc atcccgctgt
     4621 cggcggccct gctcatcgcc ctcttcggcc ggcgcctccc gggacgcgga gctgagctcg
     4681 ggatcctcgc gaccggcgtc tcgttcctcc tctcgctcgc ggtcgccggc gccgtgatcg
     4741 gtgaccacga gttccatctg gagcgctccc tgacgtggtt ccgcttcggc tcgttcgagc
     4801 tcgagctcgg gatgaacatc gacggactgg ccgcgatgat gttcgtggtc gtcaccaccg
     4861 tctccctgct cgtgcagatc tactcgaccg gctacatgaa gggcgagccc cgcttcacct
     4921 ggtacttcgc ggcgctcaac atgttcaccg gctcgatgct gaccctcgtc atcgccaaca
     4981 acctcttcca gctgctcgtc gggtgggagc tcgtcggcgt ctgctcgtac ctgctgatcg
     5041 gccacttctg ggaggagaag ccgaaccagg acgcggcgat gaaggcgttc atcacgacgc
     5101 gcaccggcga cgtcggtttc ctgttcggga tcttcgtgct gaccttcgcc gccggcagcg
     5161 cgaacatcgg ccacatcagc gagatggccg agcacggcga gatcgccgcg ggcgtgctca
     5221 cggccggcgc cctgctgctg ttctgcggtg cgatcgggaa gtccgcgcag ttcccgctcc
     5281 acgtctggct ccccgacgcg atggcaggtc cgacccctgt ctccgcgctg atccacgccg
     5341 cgacgatggt ggtcgccggc atctacctga tcgcgcggat gttccccgtc ttcatccacg
     5401 cgggtgaggt ggccctccag ttcgtctcga tcatcgccgg catcacgatg ttcatcgcgg
     5461 cgctcttggc cgtgatccag gacgacatca agcgcgtcct ggcctattcg acgatctccc
     5521 agctcgccta catggtcgcg ggtctctcgc tcggggagca cggctacacg cccgggctgt
     5581 tccacctctt cacgcacgcg ttcttcaagg ccctgctctt cctcggtgca ggctccgtga
     5641 tccacgcggt ccactcgaac aacatgagcg acatgggcgg cctccggcgc tcgatgcccg
     5701 tgacgttctg gacgttcctg atcggctcgg cggcgctcgc cgggatccca ccgctagcgg
     5761 gcttctggtc gaaggacgag atcatcacga cggcgttcgt ggaaggccac tacgtcctgt
     5821 gggcggtcgc gttcgcgacc gcgatcctca ccgccttcta catgacgagg gcggtcctac
     5881 tgaccttctt cggcgactac cgcggccacg gtcacccgca cgagtccccg cgggtgatga
     5941 ccgtgcccct cgtgatcctc gccgtgctgt cggtgtcggt cgggttcctc aacgcgacgc
     6001 cgttcgggat ccacctgttc gcggactggg tcttcttcca cgagccgcac cacgcggcct
     6061 tcaactacgg cttcgccgcg atcgcgatcg tcggggcggc gctcgggatc ggcgtcgggt
     6121 atcgcctcta cgccggctgg cgcgaccgtg acccggtccg caagctcggc cccctctacc
     6181 gcttcctcga ccgcaagtac ctgctcgacg acatctacct gcgcggcatc gtgcgtccga
     6241 tccagtaccc gatcgcccgt gcggtcaact ggacgaaccg ctacatcctc gatgggatcg
     6301 tccacctggt tgcctgggtc acccgcaagc tcgggggcgt gacggcggcc gtcgaccgca
     6361 acctcgtcga cggcgccgtg aaccgcgtgg cgatcgggac cgggtggacg ggcggactcc
     6421 tgcggtacgt gcaatccggg aacgtgcagc gctacgcggt cctgctgttc accggtgtgg
     6481 tcatcctgac ggtggtcttc acgagagtgt aaggagcgag acgtggactg gttcgacgat
     6541 tgggcgatca ccctcgcggt gttcatcccc ctggtggggg cgctgttcgt gtccctgctg
     6601 ccgaggcgcg ccgatcggct gatccgcgcg atggggatcc tgttcaccgc ggtgccgctc
     6661 gtcatcggca tcgccatgct cttccgcttc gacttcgggg cgcccggcgc gttcggggcg
     6721 gcccgcgcgg ccgacgcgct ccagttccac gtggacgtct cctggatcca ggcgatcggg
     6781 gctcgctacc acatcgggct cgacgggatc agcctcccgc tcttcgagct cacgctcttg
     6841 ctctcgttcc tctgcatgat ctacacctgg aagttcctgc cggagcccgg ccggccgaag
     6901 gcgttcatcg cgctgctcct cctcctcgag acggggatgg ccggctcgtt catcgccttc
     6961 gacctgatcc tgttcttcgt gttctgggag ctcgtgctcg tcccgatgta cttcctgatc
     7021 ggcgtctggg gcggtccgaa ccgcgagtac gcggcgatca agttcttcct ctacaccctg
     7081 ttcggcagca tcttcatgct gctgggcttc ctcgcgatgt acttccgcgc cgacccgaac
     7141 accttcgaca tgatcgcgct ccagagcgcg agcttcccgc acgcgttcca ggtcgtcgtc
     7201 ttcggcgggc tgttcctcgg gctcgcgatc aaggttccga tgtggccgtt ccacacgtgg
     7261 ctgcccgatg cgcacaccga ggcgccgacc gtcggctcgg tgatcctcgc cgggatcctg
     7321 ctgaagatgg ggacctacgg gttcgtgcgg atcgcgctgc cgatcctgcc ggacggcgcg
     7381 aaggtctggg ccccctggat cggcatcctc tccgtgatcg cgatcatcta tgccgccctc
     7441 gcctgtctcg cccaacgaga tatgaagcgg ctcatcgcgt tctcctcggt cgggcacatg
     7501 ggcttcgtga tgctcggcat cgcgaccctc acggacatcg ggatcaacgc ggcgatcttc
     7561 ggcatggtcg cccacgggat catcacgggg atgctcttct tcctcgcggg atcggtccat
     7621 gagcggtacc acacgcgcga gttcggcgag atcggcggcg ggatgctgaa gaaggtgccg
     7681 tacctcggga gcgccttcgc ctacaccgcg atcgcctcgc tcggccttcc gggcctcgcg
     7741 ggattctggg gtgaggtcat ggccctcgcc ggctcgttcc agcccgcgga ggggctatcg
     7801 gtcgggctgt tccggacgct gatggtcctc ggcgggatcg gcacgatcct cacggcgggc
     7861 tacatgctct ggatgatcca gcgcgtgaac ctcgggaccg ttccggaccg ctgggagggg
     7921 aaggccttct ccgacatcac ggccgtcgag tacgtcgcct ggacgccgct cctcatcggg
     7981 atcctgctcc tcgggatcgt ccccgccctg ctgttcggga tgacgagccc ggccgtcggg
     8041 tcgctcaccg ggatgttcta gatgctgccg cagatcgact accgcgcgat cgcgcccgag
     8101 ctgatcctcg ccgggacgat catggtcgtg ctcgtggtcg atctgttcct gcgcgagcgc
     8161 cggaagcact ggtcgatgac cgtctcgttc gtcggggtcg ccaccgcgct cggttccacg
     8221 atcccgctgt tcgggcgcga agggccggcc ttcggtgaga cgtacgtcgt cgatccgttc
     8281 gcggtcctgc tcaagatctt cttcctcgcg atggccctcg gcgtcctctt cctgtcgcag
     8341 cgctacttcc gcgaggggcg gttctaccag ggggagtact acttcctgct cctgacgtcg
     8401 ttcctcggga tgctgacgat ggcgtcctcg cgcgatctcc tcatgctgtt catctcgctc
     8461 gagctggtct ccgcccccgg gttcatcatc gccgggttgc gcaagcgcga cccccggtcg
     8521 aacgaggcag ccctgaagtt cttcctgatc gggatcctcg ccgtcgccgt gatgctctac
     8581 gggatgagcc tgctctacgg ggtcaccggg acgatcgccc tcgaggggat cgctcggtcg
     8641 ctccagcagg gggcggagcc gctcgcgatc gcggcgctcc tgttcgtcgt cgtcggcttc
     8701 gcgttcaaga tctccgcggt gccgttccac ttctgggcgc cggacaccta cgagggctcg
     8761 ccggtgccgg tcgcggcgta cctgtccacg gcatcgaagg cggcggggtt cgccggcctc
     8821 ctccagatca tgttcgtcgc cttccgcccg gtcgcggacg tgtgggctcc ggtcttcgcc
     8881 gtgctctcga tcctcacgat gacgctcggg aacctgatcg cgatccagca gcgccaggtg
     8941 gtccgcctgc ttgcctactc gagcatcgcc caggcgggct acatgctcct gccgttcgcg
     9001 ctcgtcgggc tcagtcccga cctcgaccag cgggcgttcg gcgcggcggt cctctacatc
     9061 ctgatctacg gcgccacgaa cctcggggcg ttcggggtcg tgacgtccgt gtcccgcgag
     9121 tcgcccggcg tcctcgtcga ggacttcgcc gcgctcggcc agcgagctcc ggggctctcg
     9181 gtggcgatga cgacgttcat gatctcgctc gccggcatcc ccccgaccgc cgggttcatc
     9241 gggaagctct tcatcttcct cgtcgcgatc agcgcgtggc aggccggctc cacgatcgcg
     9301 ctcgtcctcg cggtcgcgat ggtcatcaac tcggtgatca gcctctacta ctacgcggcc
     9361 gtcgtccgcc agatgttcct cgtgcccatc gagcgccccg gcaggttccg ctcgccggtg
     9421 ctcgtcaccg gagtcgtcgc cgccagcctg gtggtcgtcc tcgccgtcgg catcttcccc
     9481 gacctcttcg cgagcttccc cccggacgcg acgctcccgt agaaccgcca cgccgtcgcc
     9541 tccgtacaac gagcaggagg aggcgccatg cccgggaaga gactcgcgtc cgtgatgctc
     9601 gcgacgatcc tgctcgtgac gggatcccgc ggcgcagccg tggcgacgag ctacccctcg
     9661 tcggaggatc tgtcgatcct gaccggaccg tgcgcgatcg tcgttccctc cggcttcgag
     9721 tggctgtctc ggctg
//
LOCUS       DATLGG010000325         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_220821, whole genome shotgun sequence.
ACCESSION   DATLGG010000325 DATLGG010000000
VERSION     DATLGG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220821"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VM638_09220"
     CDS             <1..219
                     /locus_tag="VM638_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12636.1"
                     /translation="VQELAHLVRAAGDTDLAGRLERAVTDNVKLLALTIPERAAILNV
                     LDDPPDELAELRGVLMTDMLWRRDHGLE"
     gene            complement(225..461)
                     /locus_tag="VM638_09225"
     CDS             complement(225..461)
                     /locus_tag="VM638_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12637.1"
                     /translation="MVNEDGQEVIPYGTLVKLGMVLDDLGRIHCWAGSTMDRLCDLLS
                     ELAGPMPSDEAADAFWDGFQRDVVEALEEEAADE"
     gene            complement(774..965)
                     /locus_tag="VM638_09230"
     CDS             complement(774..965)
                     /locus_tag="VM638_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12638.1"
                     /translation="MTFLRAEPPAVVIILALEEPARVLIKSENDGEKARLLDWINTHD
                     ELLALVHQARTLAEKETTR"
     gene            complement(962..2242)
                     /locus_tag="VM638_09235"
     CDS             complement(962..2242)
                     /locus_tag="VM638_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVM12639.1"
                     /translation="MSDIEFAPDMPTFAHSSVTIEEFVARPVAIPEPLIGGADTRLLP
                     AASTAVFYGEGGSGKTTLTIDLALHLAAGVEWLGFEIAKPVRVMLLENEGPIDEYQLK
                     LDRKLLSWNGPKPDGRLRVHDRPWGRVDLRADVAVEGLAKQIRLHEIDVLIAGPVRRL
                     GLEGGGTPAETVAYMQLLDRVRHLAGRRVAIVNVHHENKGGDISGAFEAEFDTVVHVR
                     ADGRDRTRLVFRKSRWSSKIHRARMTLSWVRDSEGFAIIESDLDGPQPNERNEEEAAA
                     LDWLTAYIAAHPGVARGKAETAYHDAHAGGRNLARRIINSQIALATQVAESSDPDGDR
                     RNLLRFATGPGETKRGTYLYPLSHAVSPLAATLSGETGETLATPGSIGVLATSPPPIE
                     EAAAGETRGEGDEIDYDELDRLFREHADIAEGRP"
BASE COUNT          323 a          782 c          805 g          380 t
ORIGIN      
        1 gtccaagagc tcgcccatct cgtccgcgcc gccggcgaca ccgacctcgc cgggcgtctc
       61 gagcgggccg taaccgacaa cgtcaagttg ctcgcgctca ccatcccgga gcgggcagcg
      121 atcctgaacg tcctcgatga cccgccggac gagctcgccg agctccgcgg cgtcctcatg
      181 acggacatgc tctggcgccg cgaccacgga cttgagtagc gcactcactc gtccgccgcc
      241 tcctcctcca gcgcctcgac gacgtcccgc tggaacccgt cccagaatgc gtccgcagcc
      301 tcgtccgacg gcatcggccc agcaagctca ctgaggaggt cgcacagacg atccatcgtc
      361 gacccagccc agcagtgaat ccgccccagg tcgtccagca ccatcccgag cttcacgagc
      421 gtcccgtacg ggatcacctc ctgaccgtcc tcgttcacca cggccgagac ctccggccgc
      481 gccgacggcg cgccaaccgc gcccccgccg cccggttgca gtagcggtgc tccgcgcccg
      541 catactcccg cgccgtcccg tccacgtgcc ccagatccca cggctcagac gggtgaatga
      601 gatccccgca gcggacgcac gccacattcc ccagcgccac ccgcttcgcc caaacctcgc
      661 ggactctccg gtgctgacgg ccgtacaacg ccacgatcag tccacctccc ccgaccttcg
      721 gggagaaaag gacaaagcgc gggtatgtcc gcggcggagc ctaaaaaacg cggtcaccgg
      781 gtggtctcct tctcggcgag ggtgcgtgct tggtggacga gcgcgaggag ttcgtcgtgc
      841 gtgttgatcc agtcgaggag gcgtgccttc tcgccgtcgt tctcggactt gatgaggacg
      901 cgggcgggtt cctcgagggc gaggatgatc acgacggccg gcggttctgc gcgcaggaac
      961 gtcatgggcg gccctctgcg atgtcggcgt gctcgcggaa gaggcggtcg agctcgtcgt
     1021 agtcgatttc gtcgccctcg ccgcgggtct cgccagccgc cgcctcttct ataggcggcg
     1081 gcgaggtggc gagtaccccg atcgagccgg gggtcgcgag ggtttcgccg gtctcgccgc
     1141 tgagcgtggc ggcgagtggc gagaccgcat ggctgagcgg atagaggtag gtgccgcgct
     1201 tagtttcgcc ggggccggtg gcgaacctga ggaggtttcg ccggtcgccg tcaggatcgg
     1261 agctttcggc gacctgggtg gcgagtgcga tctggctgtt gatgatgcgc cgggcgaggt
     1321 tccgtccgcc ggcgtgcgcg tcgtggtagg cggtctctgc cttgccgcgc gcgacgccgg
     1381 ggtgggctgc gatgtaggcg gtgagccagt cgagtgcggc ggcttcctcc tcgttgcgct
     1441 cgttcggctg cgggccgtcg aggtcggatt cgatgatcgc gaagccttcg ctgtcgcgga
     1501 cccacgagag ggtcatgcga gcgcggtgga ttttgctcga ccagcgggac ttgcggaaga
     1561 cgaggcgggt gcggtcgcgg ccgtcggccc ggacgtggac gaccgtgtcg aactcggcct
     1621 cgaaggcgcc gctgatgtcg ccgcccttgt tctcgtggtg cacgttgacg atcgcgacac
     1681 ggcggccggc gagatgccgg acgcggtcga ggagctgcat gtacgcgacg gtctctgccg
     1741 gggttcctcc gccttcgagc ccgaggcgac ggacggggcc agcgatcagg acgtcgatct
     1801 cgtggagccg gatctgcttg gcgagccctt cgacggcgac gtcggcgcgg aggtcgacgc
     1861 gtccccaggg gcggtcgtgg acgcgcagcc ggccgtcggg cttcgggccg ttccagctca
     1921 ggagcttccg gtcgagcttg agctggtact cgtcgatcgg cccctcgttc tcgaggagca
     1981 tgacacgcac cggcttcgcg atctcgaacc cgagccactc gacgcccgcg gcaaggtgga
     2041 gcgcgaggtc gatcgtgagg gtggtcttcc cggagccgcc ctcgccgtag aagacagcag
     2101 tcgatgctgc cggcaggagc cgggtgtcgg cgccgccgat gaggggctcg gggatcgcca
     2161 cgggccgcgc gacgaactct tcgatcgtga ccgagctgtg cgcgaacgtg ggcatgtccg
     2221 gtgcgaactc gatgtcgctc acgcggcgtc ctcgccgacc gtcatgtctg ggacctcgtt
     2281 gaagtcctcg
//
LOCUS       DATLGG010000326         4343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_221523, whole genome shotgun sequence.
ACCESSION   DATLGG010000326 DATLGG010000000
VERSION     DATLGG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221523"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VM638_09240"
     CDS             <1..608
                     /locus_tag="VM638_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12640.1"
                     /translation="RVDLSGYLALHVNEVRPDGSARVTATMQAPSLVVNGRSRTPRRL
                     RTTMLFDRDGLLVKGGTFTLPQGLVARIVPIGLAPPRPPGEIAPGRSWSLSQRSADAG
                     TTFRTTGQASFSGYEDDAETVAILDVRGRTRVTGGTRHPRGRYRVQESVGLDHRAGEV
                     RWLRSVVDFDLRVSSPVSLRASGFPGVFRGSGNASIELSPV"
     gene            complement(612..2186)
                     /gene="serA"
                     /locus_tag="VM638_09245"
     CDS             complement(612..2186)
                     /gene="serA"
                     /locus_tag="VM638_09245"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HVM12641.1"
                     /translation="MRVLVTERLAERALSTLRAAFEVDVRPDLAAAGLREAIPGYAAL
                     IVRSQTTVDADVIEAADELKVIARAGIGLDNVDVATATRRGVMVVNAPQSNVLSAAEH
                     TMALLLALARNVPQAHTALVGGAWERERFQGVELHGKVLGILGLGRVGTMVAQRALAF
                     GMRILAYDPYVSRERAHQLGVEIASELEAVLTEADFLTIHLPRNPETEGLIGGPELAL
                     MKPTARLVNTSRGGIVGEGALVEALRGGVIAGAAVDVFEGEPVTEHPLFGIENVVVTP
                     HLGASTAEAQEKAGAAVAEMVALALRGEFVPYAVNVPAAEVSEAVRPFLPLAERLGRL
                     LTALAEGALRSLSAGYLGQVAEHDTAVLTLAALKGVLGGIVDEPVSYVNAPLIARDRG
                     LSLSESRSSVAQDYVNQIVLTAETDQGPLAVGGTLVGTRNAERLVRVYDFDIDMAPAE
                     HMVFFLYEDRPGVIGTVGTILGEAGVNIAAMEVGRREAGGVALMGLTVDSPIPEEALE
                     RIVAAVGMKSARSIAL"
     gene            complement(2209..3360)
                     /locus_tag="VM638_09250"
     CDS             complement(2209..3360)
                     /locus_tag="VM638_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HVM12642.1"
                     /translation="MEKPEILMTPGPTPVPQQVLAAQGGPLVYHRGPGYGSVLREVTT
                     GLRSMLKTEGDVLLFSASGTGGLESAVANCFSPGDRVVVPVAGYFGQRFAKIAEAFGL
                     DVRVVEYEWGRAVKTADIEAALAEAPTKGVLAQHSETSTGVIHDIEALARVTKEAGAL
                     FVVDVISSLGAVPYEGDAWGIDVAVGGSQKALGATPGLAFVSVSEDAWAASREAKNPR
                     FYFDWAAYKASVDAADPESPWTPAISLVLGLREAIRLYEAEGEEAVLRRHRLLSEATK
                     EGVRALGLDLDGEHPERAWAVTAVRAPEGIDGNALVAKVRADHGIILAPGQGPLKGKV
                     FRIGHLGFYDRLDILRCLAALELTLADMGYPVKRGAGVAAAEEVFAAAS"
     gene            complement(3413..>4343)
                     /locus_tag="VM638_09255"
     CDS             complement(3413..>4343)
                     /locus_tag="VM638_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVM12643.1"
                     /translation="GADPAELAITTNVTMALASVLSCLDLSGERNRIVLTELDFPTDG
                     HVALAHRARGAEVVFLPSPDGLTVPTEAFAEAIDERTALVIINRVLYRSSALLDVKEI
                     CRIAREAGAWTVIDDFHGAGIVPIDVHDVGCDFYTTGVLKWLCGGPGLTFLYARKDLL
                     PSLEPSIAGWWSQREPFAFRTQELDWHPSARRFETGTHPAPTAFIGHGGLEIIAEVTP
                     ERIRERVGELGDALIERAEAEDFPVRSPRDRRLRGGVINIGLGPDAGKIAEALYERDV
                     CVDHRGDGIRVSPHFFNTEQDLDRLIQELRDLR"
BASE COUNT          614 a         1612 c         1529 g          588 t
ORIGIN      
        1 tgcgggtgga cctgagcggc tacctggcgc tgcacgtgaa cgaggtccgg cccgacggca
       61 gcgcccgggt gaccgccacg atgcaggcac cctccctcgt cgtgaacgga cggtcgcgga
      121 cgccgcggcg cctgcgcacg acgatgctct tcgaccgcga cggcctcctc gtcaagggcg
      181 ggacgttcac cctcccgcag ggcctcgtcg cccggatcgt gccgatcggc ctcgcgcctc
      241 cacgtccccc gggggagatc gccccggggc ggtcgtggtc cctctcccag cgctccgcgg
      301 acgccggcac caccttcagg acgaccgggc aggccagctt ctccggctac gaggacgacg
      361 ccgaaaccgt cgcgatcctc gacgtccggg gccgcacgcg ggtcaccggc ggaacgagac
      421 acccgcgcgg gcgctaccgc gtccaggagt cggttgggct cgaccaccgt gccggcgagg
      481 ttcgctggct ccggagcgtc gtcgacttcg acctccgggt cagctcgccc gtgtcgctgc
      541 gggcctccgg gttcccgggc gtcttccggg ggtcgggcaa cgcgagcatc gagctcagcc
      601 cggtctgagc ctcagagcgc gatcgaccga gctgacttca tcccgaccgc ggccacgatc
      661 cgctcgagcg cctcttcggg gatcgggctg tcgacggtga gccccatgag cgcgacgcct
      721 cccgcctcac gccgcccgac ctccatcgcc gcgatgttca ccccggcctc gccgaggatc
      781 gtacccaccg tcccgatgac cccgggccgg tcctcgtaga ggaagaagac catgtgctcg
      841 gccggcgcca tgtcgatgtc gaagtcgtag acgcgcacga gccgctcggc gttccgcgtg
      901 ccgacgaggg tgccgcccac cgccagcggc ccttggtccg tctcggcggt gagcacgatc
      961 tggttcacgt agtcctgtgc gaccgagctg cgggactccg acagggacag cccccggtcg
     1021 cgcgcgatga ggggcgcgtt cacgtaggag accggctcgt cgacgatgcc gcccagcacc
     1081 cccttcaagg ccgccagcgt gaggaccgcc gtatcgtgct cggccacctg tccgaggtag
     1141 ccggccgaca gcgaccgcag cgctccctcc gccagcgccg tcagcagccg tccgaggcgc
     1201 tcggccagcg gaaggaaggg gcggacagcc tcgctcacct ccgcggccgg gacgttgacc
     1261 gcgtagggga cgaactcgcc gcggagcgcg agggccacca tctcggcgac cgccgcgccg
     1321 gccttctcct gcgcctcggc ggtcgaagcg ccgaggtgcg gcgtcacgac cacgttctcg
     1381 atcccgaaca gcgggtgctc ggtcaccggc tcgccctcga agacgtcgac ggcggccccg
     1441 gcgatcacgc cgccgcgcag cgcctcgacg agcgccccct ccccgacgat cccgccacgc
     1501 gaggtgttca cgagccgggc cgtgggcttc atcagcgcga gctcggggcc gccgatcagg
     1561 ccttcggtct ccgggttgcg cggcaggtgg atcgtgagga agtcggcttc ggtgagaacg
     1621 gcctcgagct cggaggcgat ctcgacgccg agctggtggg cgcgctcgcg cgacacgtag
     1681 gggtcgtagg cgaggatccg catcccgaag gcgagcgcac gctgggcgac catcgtgccg
     1741 acacggccca gcccgaggat gccgaggacc ttgccgtgca gctcgacgcc ctggaaccgc
     1801 tcccgctccc acgcgccgcc cacgagcgcg gtgtgcgcct gcgggacgtt gcgcgcgagc
     1861 gcgagcagga gcgccatcgt gtgctcggcc gccgacagga cgttcgactg cggcgcgttg
     1921 acgaccatca cgccgcggcg ggtggcggtc gcgacgtcga cgttgtcgag cccgatgccg
     1981 gcgcgggcga tcaccttcag ctcgtcggct gcttcgatga cgtccgcgtc gacggtcgtc
     2041 tgcgaacgga cgatcagcgc ggcgtagccg gggatcgcct cgcgcaggcc cgcggccgcg
     2101 aggtccggcc ggacgtcgac ctcgaaggca gcgcgcagcg tcgacagcgc acgctcggcc
     2161 agccgctcgg tgacgaggac ccgcatcgcc gcgagtatag gtgcggcctc aggaggccgc
     2221 ggcgaagacc tcctcggccg ccgcgacgcc ggcgccccgc ttcaccggat agcccatgtc
     2281 cgcgagggtc agctcgagcg ccgcgaggca ccgcaggatg tcgagccggt cgtagaagcc
     2341 gaggtgcccg atgcggaaga ccttgccctt caacggcccc tgtcccgggg cgaggatgat
     2401 cccgtggtcc gcgcggacct tcgccacgag cgcgttcccg tcgatcccct ccggcgcccg
     2461 cacggccgtg acggcccacg cccgctcggg gtgctccccg tcgaggtcga ggccgagcgc
     2521 gcggacgccc tccttcgtcg cctccgagag gaggcggtgc cggcggagca cggcctcctc
     2581 gccctcagcc tcgtacaggc ggatcgcctc gcgcaggccg agcaccaggc tgatcgccgg
     2641 cgtccaaggg ctctcgggat cggccgcgtc gaccgaggcc ttgtacgccg cccagtcgaa
     2701 gtagaagcgc ggattcttgg cctcgcggga ggcggcccag gcgtcctcgg aaacgctgac
     2761 gaacgccagt ccaggcgtgg cgccgagcgc cttctgcgac ccgccgaccg cgacgtcgat
     2821 cccccaggcg tcaccctcgt aggggacggc gccgagcgag gagatcacgt cgacgacgaa
     2881 cagcgcgccc gcctccttcg tgacgcgggc gagcgcctcg atgtcgtgga tcacaccggt
     2941 cgaggtctcg gagtgctggg cgaggacgcc cttcgtcggg gcctcggcga gcgcggcctc
     3001 gatgtccgcg gtcttcacgg cacggcccca ctcgtactcg acgacgcgca cgtcgagccc
     3061 gaacgcctcc gcgatcttcg cgaaccgctg cccgaagtac ccggcgacgg ggacgacgac
     3121 acggtcgccc ggcgagaagc agttggccac cgcggactcc aggccgccgg tgccggaggc
     3181 gctgaacagc agcacgtcgc cttcggtctt gagcatcgac cggagcccgg tcgtgacctc
     3241 ccgcagcacg gatccgtacc ccgggccgcg gtggtagacg agcggcccac cctgcgccgc
     3301 cagcacctgc tgcggaaccg gcgtcggccc cggcgtcatc aggatctcag gcttctccat
     3361 gcgtcagctt ccccctcgct cgctcactgg cggccagcgt agcaccggcc tcctagcgca
     3421 ggtcgcggag ctcttggatg aggcggtcga ggtcctgctc ggtgttgaag aagtgagggg
     3481 agactcggat gccgtcgcct cggtggtcga cgcagacgtc gcgctcgtag agggcctcgg
     3541 cgatcttgcc ggcgtcgggg ccgaggccga tgttgatcac gccgccacgc aggcgccggt
     3601 cgcggggcga tcggaccggg aagtcctcgg cctcggcgcg ctcgatcaga gcatcgccga
     3661 gctcgccgac gcgctcgcgg atccgttcgg gcgtgacctc ggcgatgatc tccagcccgc
     3721 cgtggccgat gaacgcggtc ggcgcggggt gcgtgccggt ctcgaaacgc cgggccgacg
     3781 gatgccagtc gagctcctgc gtgcggaagg cgaacggctc acgctggctc caccagcccg
     3841 cgatcgaggg ctcgagcgac ggcagcagat ccttccgcgc gtacaggaag gtcagccccg
     3901 ggccgccgca gagccacttc aggactcccg tcgtgtagaa gtcgcaaccg acgtcgtgaa
     3961 cgtcgatcgg gacgatgccc gccccgtgga agtcgtcgat caccgtccac gccccggcct
     4021 cccgcgcgat gcggcagatc tccttcacgt cgaggagcgc gctcgaccgg tagagcacac
     4081 ggttgatgat cacgagcgcg gtccgctcat cgatcgcctc cgcgaaggcc tccgtcggaa
     4141 ccgtcagccc gtcggggctc gggaggaaca ccacctcggc cccacgcgcg cggtgcgcca
     4201 gcgcgacgtg gccgtcggtg gggaagtcga gctcggtcag cacgatgcgg ttgcgctccc
     4261 ccgacagatc gaggcacgag agcaccgacg cgagcgccat cgtgacgttc gtcgtgatcg
     4321 cgagctcggc cgggtccgcg ccg
//
LOCUS       DATLGG010000327         8674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_124789, whole genome shotgun sequence.
ACCESSION   DATLGG010000327 DATLGG010000000
VERSION     DATLGG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124789"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VM638_09260"
     CDS             <1..446
                     /locus_tag="VM638_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12644.1"
                     /translation="VAHMKNTFHTKAEADAKFVPAASFFTYKQTLLPSDVEPLGQFGN
                     FRLRGRCEGDGTSMRVRVQIRTTVDGAVLRGEESGHFRPLNTGDGWVRWSAGWYNDEG
                     APRLNNPVEPAHAIGPDGTAIYGTSTLGANIPEGTCTVAGFIINM"
     gene            complement(503..1456)
                     /locus_tag="VM638_09265"
     CDS             complement(503..1456)
                     /locus_tag="VM638_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVM12645.1"
                     /translation="MTAGGSILVTGAAGFIGSHLCDRLLAEGRRVIGADNLATGRIAN
                     LAEARSYGKAFSFENVDVRAEVLPALFERHRPEVVFHLAAQAGVRPSLEDPKHDAEVN
                     IVGSLNLLEAAAASGVRKVVYAASGGTMYGEPRRLPVKESAMGRTHPQTPYGISKRVV
                     DDYLRFYQRYRGIDFTALALGNVYGPRQDPAGEAGVIAIFAKKMLAGETPMIYGDGNQ
                     TRDYVFVSDVVHAFSLAIERGSGRVLNIGTGLETSVNHLYRMLAQIIGFVPEPERGPL
                     PAGEPRRIVLDATLAEGELGWRPWTQLEEGLLETVAWLKGV"
     gene            complement(1453..2457)
                     /gene="tsaD"
                     /locus_tag="VM638_09270"
     CDS             complement(1453..2457)
                     /gene="tsaD"
                     /locus_tag="VM638_09270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVM12646.1"
                     /translation="MKILGIETSCDETAAAVVEDGLKVLSNVLASQHDIHATYGGVVP
                     ELASRAHVERLNTVIDRALEEAAIGWREVEAVAVTRGPGLVGALLVGLATAKAAALAR
                     GVPLVGVNHLEGHVYANFLEHGPPEPPYVALIVSGGHTLLVHMPDLHRYEILGQTLDD
                     AAGEAFDKIARFLGLGFPGGPALDRLAREGDPQAIRFPRAMADSGTYDFSLSGLKTAV
                     VRHVRREQEARNELSLPDLAASFQEAIVDVQVQKTMDAAEAMGVRTVLLGGGVVANSR
                     LRERMAAEAEGRGVRLLSPPLELCTDNAAMIASAGYFRLQRGEILPFDAGADPGLPLS
                     "
     gene            complement(2454..3005)
                     /gene="rimI"
                     /locus_tag="VM638_09275"
     CDS             complement(2454..3005)
                     /gene="rimI"
                     /locus_tag="VM638_09275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HVM12647.1"
                     /translation="MRRSTGLEERTSPEIEIVPMRRRHIRGIMAIERQVYPRPWSPNL
                     FMSEMHAGSTRAYIVAQMGSRVVGYGGLMCYGEEAHVTTIAVDPEHHRLKIGSRLLYE
                     LIENARGMGAHAVSLEVRITNWAAQRMYGKFGFRPVGVRKNYYQETGEDALVMWTEDI
                     RTPEYTRLLRGIVAQLPEGVRPA"
     gene            complement(2965..3669)
                     /gene="tsaB"
                     /locus_tag="VM638_09280"
     CDS             complement(2965..3669)
                     /gene="tsaB"
                     /locus_tag="VM638_09280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVM12648.1"
                     /translation="MLALAIETSTPQTSVALGTEQGVLSSASVSWARGHSEVVVPAIE
                     QVLEWSETDLSQLGGVAVGLGPGLFTGLRAGVATARALAQVLNVPVVGIPSLDVLAFA
                     VRQSRWRIGAVLDGKRGEVFYAFYRPVPGGVTRETDYGVGPPERLAAELAASREETLL
                     VGNGALLYARDLEDAATPVEMASASTAFPDARALLELAIPRFHREETSRFDEVVPMYV
                     RRSDAEINWSRRANIA"
     gene            complement(3670..4164)
                     /gene="tsaE"
                     /locus_tag="VM638_09285"
     CDS             complement(3670..4164)
                     /gene="tsaE"
                     /locus_tag="VM638_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVM12649.1"
                     /translation="MSGRLELATSAPEETRRVGAALAGLLVPGDVVSLSGELGAGKTC
                     LVQGAAAALGVTDPVASPTFVLVREYRGSLPVYHLDVYRLERLQEVIDLGYEDLLDPR
                     GVMFVEWGDAIEALLPESHLDLRIEGTDDESRRRMLIRGYGRGWAERWERLEDALSPW
                     GVAA"
     gene            complement(4164..4793)
                     /locus_tag="VM638_09290"
     CDS             complement(4164..4793)
                     /locus_tag="VM638_09290"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVM12650.1"
                     /translation="MSRTLEEVAAVATGCTRCRLSARRTQVVFGHGNPHADLMFVGEG
                     PGHHEDVQGVPFVGAAGQLLNRLLAGIGLRREEVYITNVVLCRPPNNRDPLPDEIEAC
                     SGYLMEQVAAVDPRVIVTLGNFATRVILGRQVGISRIRGTRETWSGRTVVPMFHPAAA
                     LRSGGERSPTHQALVNDFALVRRILDETPPEPPSPAPVSVGAQEQLGLF"
     gene            complement(4790..5677)
                     /locus_tag="VM638_09295"
     CDS             complement(4790..5677)
                     /locus_tag="VM638_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HVM12651.1"
                     /translation="MITDVAGIRVGHWTDPIGRTGCTVVLPPPGTVASGEVRGGAPGT
                     RETDLLRPGMLVQHADAILLSGGSAFGLAAADGVVRWLEEDGRGVDAGVARVPIVPAA
                     VLFDLGVGDPGARPDAAAGYAAAAAAGTSVEEGQVGAGTGATVGTVAGPERAVPGGIG
                     SAARAAGGVTVGAIAAVNAYGVVVDDEGAPIAGALPADGSEGWTPFRPPPATNTTLAV
                     VATDARLSRERAHLLAVAAHDALARAIEPSHTMWDGDTIFTLATGATEGPQDAIERLA
                     EAALVEAIRRAVTAEGRRR"
     gene            complement(5674..6738)
                     /locus_tag="VM638_09300"
     CDS             complement(5674..6738)
                     /locus_tag="VM638_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVM12652.1"
                     /translation="MRRTARIAAGVGVAAAGAAVAVVAERAAFRRLRSGPDPEAGVPL
                     EALPPDDLGPVRSFDGTELAVRAAGPVGAPVLVFCHGFSLDMTTWHYQWTAFSDRYRC
                     VLFDQRAHGRSGKPPEDDYSVRAMGRDIRAVLDAVAGDAPAVLIGHSMGGMGILGFAE
                     RHPGEFGARVRGVVLADTTASDVLRQILGGVGTKIEEALRPLTRRFVQDPQRVQRIRG
                     RISSVSDLSFALMRATNFGPAASPAQVEHLAAVAARTPAEVWVHTAADIRELDLRRAL
                     EHVTVPSLVVVGDRDLVTPRGAAESLRDALPDARAVVIARAGHVTMLERHEVFNQVLT
                     RYLDEVLPPQRVGASGDGTP"
     gene            complement(6735..7835)
                     /gene="alr"
                     /locus_tag="VM638_09305"
     CDS             complement(6735..7835)
                     /gene="alr"
                     /locus_tag="VM638_09305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVM12653.1"
                     /translation="MSFRPTVAVVDLDAIAHNARRLKPSDAELMAVVKADAYGHGAVP
                     VARTALAAGASWLAVALVEEGLDLRDAGVEAPILVLTEVPRGAEKDALAAGLTPTVYT
                     GEGIARLAAAGSAGAIRCHLKVDTGMHRVGARPEDALPLCRDAAEHGLEVEGIWTHLA
                     VADEVGHEATRGQLERFGQVVEALRSAGFDPRYRHAANSAAALTLPEARFDLVRAGIA
                     LYGIAPSSEVGSEGLRRAMSLRSRVAFVKRVEAGEALSYGLRYRLERDAWIATVPIGY
                     ADGYTRRLSGRGRVLVRGVRRPIAGTVTMDQILVDCGDEEVAPGEEVVLFGRQGHEEI
                     TAEEVAEWSGTIGYEVVCAVGARVPREHRGGA"
     gene            complement(7832..>8674)
                     /locus_tag="VM638_09310"
     CDS             complement(7832..>8674)
                     /locus_tag="VM638_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVM12654.1"
                     /translation="DLVRSDVLLVDEATVRDLLPRRGTDAHKRTSGVVLVVAGSARMT
                     GAPTLVARGAYRAGAGLVHLLAPAEALPAIQPVLPEATYGTLPPTPDDIDLEAFDAIV
                     IGPGLTGDPEIPAFVRRLVAIAPKPVVLDADGLNAFAGNAGEFAAAGTDLVLTPHTGE
                     FARLFGMPVGEVLEDRIGIARKAAAETRATILLKGARSVIALPGGEVRINPTGSPALS
                     TGGTGDVLAGAIGAYLARGGFAPEAATVGAYVHGLAGELLHEGALAGDVADALPVAID
                     RILR"
BASE COUNT         1255 a         3220 c         3054 g         1145 t
ORIGIN      
        1 tcgtggcgca catgaagaac acgttccaca cgaaggcgga ggcggacgcg aagttcgtcc
       61 cggcggcgag cttcttcacc tacaagcaga cgctcctgcc cagcgacgtg gagcctctcg
      121 ggcagttcgg gaacttccgg ctgcgcggca ggtgcgaggg tgacggaacc tcgatgcgcg
      181 tcagggtgca gatccgcacg acggtggacg gggcggtcct gcgcggcgaa gagagcgggc
      241 acttccgccc cttgaacacc ggggacggct gggtccggtg gagcgccggc tggtacaacg
      301 acgaaggggc gccccggttg aacaaccctg tggagccggc ccacgcgatc ggccccgacg
      361 gaaccgcgat ctacggcacg tcgacgctcg gagcgaacat ccccgagggg acctgcaccg
      421 ttgcaggctt catcatcaac atgtgagcgt ctccggtcgg acggagcgag cgaccccccg
      481 cctaccgcgg ggggtctccg cttcacacac ccttcagcca ggcgacggtc tcgaggagcc
      541 cctcttcgag ctgggtccac ggacgccagc ccagctcgcc ctcggcaagg gtggcatcca
      601 gcacgatgcg tcgaggctcc cccgccggca gtggcccccg ctcgggctcg gggacgaacc
      661 cgatgatctg cgccagcatc cggtagaggt ggttcaccga cgtctcgagg cccgtcccga
      721 tgttcagcac gcgacctgac ccacgctcga tcgcgagcga gaacgcgtgc acgacgtcgg
      781 agacgaagac gtagtcacgc gtctggttgc cgtcgccgta gatcatcggc gtctcgcccg
      841 cgagcatctt cttcgcgaag atcgcgatga cgccggcctc cccggccggg tcctgccggg
      901 gtccgtagac gttgccgagc gcgagtgcgg tgaaatcgat cccgcggtac cgctgataga
      961 accgcaggta gtcgtcgacg acccgcttcg agatcccgta gggcgtctgc gggtgcgttc
     1021 gtcccatcgc cgactccttc accggcagcc ggcggggctc tccgtacatc gttccaccgg
     1081 acgccgcgta gaccaccttg cgcacgccgc tcgcggccgc ggcctcgagc aggttgagcg
     1141 acccgacgat gttcacctcc gcatcgtgct tcgggtcttc gagcgacggg cgcaccccgg
     1201 cctgggcggc gaggtggaac acgacctccg gacggtggcg ctcgaacagc gccggcagga
     1261 cctcggctcg gacgtcgacg ttctcgaacg aaaacgcctt gccgtacgag cgggcttcgg
     1321 cgaggttggc gatcctgccc gtcgccaggt tgtccgcgcc gatgacccgc cggccctcgg
     1381 cgagcaatcg atcgcacagg tgcgacccga tgaacccggc ggcgccggtg acgaggatgc
     1441 tgccgccagc ggtcacgaca gcggaaggcc ggggtccgcg cctgcgtcga acggcaggat
     1501 ctcgccgcgc tgcaggcgga agtacccggc ggaagcgatc atcgccgcgt tgtcggtgca
     1561 gagctcgagc ggaggcgaga ggagcctgac gccgcgtccc tcggcctcgg ccgccatcct
     1621 ctctcgcagg cgggagttcg cgacgacgcc gccccccagc aagacggttc gcactcccat
     1681 cgcctcggcc gcgtccatcg tcttctgcac ctgaacgtcg acgatcgctt cttggaacga
     1741 ggcggcgagg tccggaaggg acagctcgtt ccgcgcttcc tgctcgcggc ggacgtggcg
     1801 gacgaccgcc gtcttcaggc cggagagcga gaaatcgtac gttcccgagt ccgccatcgc
     1861 gcgcgggaac cggatcgcct gcgggtcccc ctcacgcgcg agccggtcga gcgccgggcc
     1921 ccccgggaaa ccgagcccga ggaagcgcgc gatcttgtcg aaggcctcgc cggccgcatc
     1981 gtcgagcgtc tggccgagga tctcgtagcg gtgcaggtcg ggcatgtgaa cgaggagggt
     2041 gtggccgccg gagacgatca gcgcgacgta cggcggctcg ggcggcccgt gctcgaggaa
     2101 gttcgcgtag acgtggccct cgaggtggtt cacccccacg agcgggaccc cgcgggcgag
     2161 cgccgcggcc ttcgccgtgg ccagccccac gaggagcgcc ccgacgaggc cggggccgcg
     2221 cgtcaccgcg accgcctcga cctcccgcca gccgatcgcg gcctcctcga gcgcccggtc
     2281 gatcaccgtg ttcaagcgct cgacgtgcgc gcgggaagcg agctcgggga cgaccccacc
     2341 gtacgtcgcg tggatgtcgt gctggctggc gaggacgttc gagagcacct tgagcccgtc
     2401 ctcgacgacc gctgccgccg tctcgtcgca ggaggtctcg atccccagga tcttcatgcc
     2461 ggccggaccc cctcgggcag ctgagcgacg atcccccgca gcagcctcgt gtactcgggc
     2521 gtccggatgt cctcggtcca catcacgagc gcgtcctcac ccgtctcctg gtagtagttc
     2581 ttccgcaccc ccaccgggcg gaacccgaac ttcccgtaca tccgctgggc ggcccagttc
     2641 gtgatcctga cctcgagcga gaccgcgtgg gcgcccatcc cccgggcgtt ctcgatcagc
     2701 tcgtagagca ggcggctgcc gatcttcagc cgatggtgct cggggtcgac ggcgatcgtg
     2761 gtgacgtggg cctcctcgcc gtagcacatc aggcccccgt agccgacgac gcggctcccc
     2821 atctgggcga cgatgtaggc gcgcgtcgag ccagcgtgca tctccgacat gaacaggttc
     2881 gggctccacg gccgcggata gacctggcgc tcgatcgcca tgatcccccg gatgtgccga
     2941 cggcgcatcg ggacgatctc gatctcaggc gatgttcgct cttcgagacc agttgatctc
     3001 cgcatccgat ctcctcacgt acatggggac gacctcgtcg aagcgcgagg tctcctctcg
     3061 gtggaaccgg gggatcgcga gctccagcag cgcccgggcg tcggggaacg cggtcgacgc
     3121 cgaggccatc tccaccgggg tcgccgcgtc ctccaggtcg cgggcgtaga gcagggcgcc
     3181 gttgccgacc agcagggtct cctcgcggga cgcggccagc tccgccgcga gtcgctcggg
     3241 aggcccgaca ccgtagtcgg tctcacgggt gacgccgccc gggaccggcc ggtagaaggc
     3301 gtagaagacc tcgccccgct tcccatccag gaccgcgccg atccgccagc gggattgccg
     3361 cacggcgaag gcgaggacgt ccagcgacgg gatcccgacg acgggtacgt tgaggacctg
     3421 cgcgagcgct cgcgccgtgg cgaccccagc gcgcaggccg gtgaacagcc ccggcccgag
     3481 accgaccgcg acgccgccga gctgggagag gtcggtctcc gaccactcga gcacctgctc
     3541 gatcgccggt acgacgacct ccgaatggcc acgcgcccac gacacgctcg ccgaggagag
     3601 gaccccctgc tccgtgccga gcgcgacgct cgtctgcggc gtcgaggtct cgatcgcgag
     3661 ggcgagcatc taggccgcca ccccccaggg gctcagggcg tcctcgaggc gctcccaccg
     3721 ctccgcccag ccgcgcccgt agccccggat gagcatgcgc cgccgggact cgtcgtccgt
     3781 gccctcgatc cggaggtcga ggtgggattc cggcagcagc gcctcgatcg cgtcgcccca
     3841 ctcgacgaac atgacccccc gcgggtccaa gaggtcctcg tagccgaggt cgatcacctc
     3901 ctgcagccgc tccagccggt agacgtcgag gtggtagacg gggagcgatc cacggtactc
     3961 gcgcaccagc acgaaggtcg gggacgcgac cgggtccgtc acgccgagtg cggccgccgc
     4021 gccctggacg aggcaggtct tcccggctcc gagctcgccg gagaggctga cgacgtcgcc
     4081 cgggacgagg agcccggcga gggcggcgcc cacccggcgg gtctcctccg gcgcggaggt
     4141 cgcgagctcg agtcggccgc tcatcagaag agtcccagct gctcctgcgc gccgacgctc
     4201 accggcgcgg gcgacggggg ctcgggggga gtctcgtcga ggatgcggcg gacgagcgcg
     4261 aagtcgttca cgagcgcctg atgcgtcggg ctccgctcac ccccgctgcg cagggcggcc
     4321 gcaggatgga acatcggcac tacggtccgc ccgctccacg tctcccgggt cccccggatc
     4381 ctcgagatgc cgacctgccg gccgaggatc acgcgggtgg cgaagttccc cagggtcacg
     4441 atcacgcggg ggtcgaccgc cgccacctgc tccatgaggt agcccgagca cgcctcgatc
     4501 tcgtcgggga gcgggtcgcg gttgttgggc gggcggcaca ggaccacgtt cgtgatgtag
     4561 acctcctcac gccggagacc gatcccggcg aggaggcggt tcagcagctg tccggcagcg
     4621 ccgacgaacg ggaccccctg gacgtcctcg tggtggcccg gcccctcgcc cacgaacatg
     4681 aggtcggcgt gcgggttgcc atgtccgaac accacctggg tgcggcgagc ggacagacgg
     4741 cagcgggtgc acccggtcgc gacagcggcg acctcttcca gcgtgcggct cagcgtcgcc
     4801 tcccctccgc ggtcacggcc ctccggatcg cctcgaccag cgccgcttcg gccaaccgct
     4861 cgatcgcgtc ctgggggccc tcggtagcgc cggtggcgag cgtgaagatg gtgtcgccgt
     4921 cccacattgt atgggacggc tcgatcgcac gggcgagcgc gtcgtgcgcc gcgacggcca
     4981 gcaggtgcgc ccgctcccgg gacaggcggg cgtcggtggc caccaccgcg agggtcgtgt
     5041 tcgtggccgg cggcgggcgg aaaggcgtcc agccctcgct cccgtcggcg ggcagggctc
     5101 cggcgatcgg cgcgccctcg tcgtcgacga ccaccccata cgcgttgacc gcggcgatcg
     5161 cgccgacggt caccccgccg gccgctcgcg cggcgctgcc gatgccccct ggaaccgctc
     5221 gctcgggtcc ggccaccgtc ccgaccgtcg ctccggtccc ggccccgacc tgcccctcct
     5281 cgacgctggt cccggcggcg gcggcggcag cgtagcccgc ggcggcgtcc ggacgagcgc
     5341 ccggatcccc gacgccgagg tcgaagagga cagccgccgg aacgatcggg acgcgcgcga
     5401 cgcccgcgtc cacgccccgg ccgtcctcct ccaaccagcg gacgacaccg tcggcggcgg
     5461 cgagaccgaa cgcgctgccc ccgctcagca agatggcgtc ggcgtgctgg acgagcatgc
     5521 ccggacgcag caggtcggtc tcgcgggtcc cgggcgcccc gccacggacc tcccccgacg
     5581 cgacggtgcc gggaggcggc aggacgacgg tgcagccggt cctgccgatc gggtccgtcc
     5641 agtggccgac ccggatgccg gcgacgtcgg tgatcacggc gtgccgtcgc cggaggcgcc
     5701 gacccgctgg ggaggaagga cctcgtcgag gtaccgcgtg aggacctggt tgaacacctc
     5761 gtgccgctcg agcatcgtga cgtgccccgc ccgggcgatg acgacagcgc gcgcgtcggg
     5821 gagcgcgtcc cgcagcgact ccgcggcccc cctcggcgtc acgagatctc ggtcaccgac
     5881 gacgacgagc gaaggaacgg tcacgtgctc gagcgcccgg cgcaggtcga gctcgcggat
     5941 atccgcagcc gtgtggaccc agacctcggc cggtgtccgc gccgcgacag ctgccagatg
     6001 ctcgacctga gcgggcgagg cggcgggacc gaagttcgtc gcccgcatga gagcgaatga
     6061 gaggtcgctc acgctcgaga tccgcccgcg gatccgctgt acccgctgcg gatcctggac
     6121 gaaccggcgg gtcagaggac ggagggcctc ctcgatcttc gtcccgacgc cgccgaggat
     6181 ctgccggagg acatcggacg ccgtcgtatc ggcaaggacc acgccgcgca cccgcgcgcc
     6241 gaattccccg gggtgccgct cggcgaaccc caggatcccc atacccccca tgctgtggcc
     6301 gatcagcacg gccggcgcgt ctccggcgac cgcgtcgagg acggctcgga tgtcgcgccc
     6361 catcgctcgc accgagtagt cgtcctccgg cggctttccc gagcgaccgt gcgcgcgctg
     6421 gtcgaacagg acgcagcggt agcggtcgga gaacgccgtc cactggtagt gccacgtcgt
     6481 catgtcgagg ctgaacccgt ggcagaagac gagcacgggt gctccgacgg gacccgccgc
     6541 gcgcaccgcg agctcggtgc cgtcgaaaga gcggaccggg ccgaggtcgt cgggcggcaa
     6601 ggcctcgagg gggacgcccg cctcggggtc cgggccggag cggaggcgcc ggaaggcggc
     6661 ccgttccgcc acgaccgcga ccgcggcgcc ggcggcggcg acacccactc ccgcggcgat
     6721 gcgcgcggtc cgcctcatgc tcccccgcga tgctcgcgcg gcacccgcgc cccgaccgcg
     6781 cacacgacct cgtatccgat cgtccccgac cattccgcca cctcctcggc ggtgatctcc
     6841 tcgtgaccct gtcggccgaa cagaacgacc tcttctccgg gcgcaacctc ctcgtccccg
     6901 cagtcgacga ggatctgatc catcgtcacc gtcccggcga tcggccgccg cacgccccgg
     6961 accagcacac gcccacgacc cgacagacgg cgcgtgtacc cgtcggcgta cccgatcggg
     7021 accgtcgcga tccacgcgtc gcgctcgagc cggtaccgga gcccgtacga cagcgcctcg
     7081 cctgcctcga cccgcttcac gaacgccacc ctggagcgaa gcgacatcgc ccggcgcaag
     7141 ccctcgctcc cgacctccga cgacggggcg atcccgtaga gcgcgatccc ggcgcggacg
     7201 aggtcgaacc tcgcttcggg aagcgtcagc gcggccgccg agttggccgc gtgccggtag
     7261 cgggggtcga acccggcgct ccggagggct tcgacgacct ggccgaaccg ctcgagctgc
     7321 ccgcgagtcg cttcgtgccc gacttcgtcg gcgaccgcga ggtgggtcca gatgccctcg
     7381 acctcgagac cgtgctcggc ggcatcccgg cagagcggga gggcgtcctc cggacgggcg
     7441 ccgacccggt gcatcccggt gtcgaccttc aggtggcagc ggatggcgcc ggcagacccg
     7501 gctgcggcga gacgagcgat cccctcaccg gtgtagaccg tgggggtgag gccggcggcg
     7561 agggcatcct tctccgcgcc ccgggggacc tcggtcagga cgaggatcgg cgcctcgacg
     7621 ccggcgtcgc gcaggtcgag tccctcctcg acgagcgcga ccgccagcca gctcgccccc
     7681 gcggcgagcg ccgttcgcgc gacggggacg gcgccgtgac cgtaggcgtc ggccttgacg
     7741 accgccatca gctccgcatc cgagggcttc agccgcctgg cgttgtgcgc gatcgcgtcg
     7801 aggtcgacga ccgccaccgt cggacggaag ctcaccggag gatcctgtcg atggcgaccg
     7861 gcagggcatc ggcgacgtcg ccggcgagcg cgccctcgtg cagcagctcg cccgcgagcc
     7921 cgtgcacgta ggcgccgacg gtcgcggcct ccggggcgaa cccgccgcgt gcgaggtacg
     7981 ccccgatcgc gccggcgagc acgtcgcccg tccctccggt cgagagtgcg ggtgagccgg
     8041 tcgggttgat gcgcacctcg ccccccggca gagcgatgac cgagcgcgcc cccttcagca
     8101 ggatcgtcgc gcgcgtctcg gccgcggcct tgcgggcgat gccgatccga tcctcgagca
     8161 cctcccccac cggcatcccg aacagccgcg cgaactcccc cgtgtgcggc gtgaggacga
     8221 ggtcggtccc ggccgcggcg aactcgccgg cgttcccggc gaacgcgttc agtccatcgg
     8281 cgtcgaggac gaccggcttc ggtgcgatcg ccacgagccg ccggacgaac gccgggatct
     8341 ccggatcgcc cgtgaggcct ggcccgatga cgatcgcgtc gaaggcctcc aggtcgatgt
     8401 cgtcgggggt gggcggcagc gtcccgtacg tcgcttcggg caagaccggc tggatcgccg
     8461 ggagcgcctc cgcgggcgcg aggaggtgaa cgagtccggc acccgcacgg taggcgcctc
     8521 gagccacgag cgtcggtgcg ccggtcatcc gggcagagcc cgcgacgacg aggaccacgc
     8581 cggaggtgcg cttgtgggca tcggtccccc tcctcggcag cagatcgcgc accgtcgcct
     8641 cgtcgaccaa gagcacgtcc gagcggacca ggtc
//
LOCUS       DATLGG010000328         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_124969, whole genome shotgun sequence.
ACCESSION   DATLGG010000328 DATLGG010000000
VERSION     DATLGG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124969"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1473)
                     /locus_tag="VM638_09315"
     CDS             complement(100..1473)
                     /locus_tag="VM638_09315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVM12655.1"
                     /translation="MTATARLSGQRRGVAALGVLCAAQFMLVVDVVVVNVAIPSIRAD
                     LGLPDSRLQLIAVAYTLTFGSLLIWFGRMGDVFGRRRLFLAGLAVFTLASLATGLAHS
                     GWSLVAARACQGVGAAMASPSALALLMVTFEEDGARGRALGYWGAVGSGGAIVGQLLG
                     GALTDVLGWRSIFLINVPIGIATIALATRHLQESRPAEATRVHSRGAILLTAGLAAAT
                     LTLTRIGEGASATTTAVGAGVACALLAAFAVSERRHDEPLVDHALLRTRQVVTGNVLL
                     AVNAGTLGAALFFTTLYLQVGLGYSPLAVGAAFAPITLLVLLISPRIGARIASVGARQ
                     LLSIGFGFAAVGTALLSRLPDDGSYLRDVLPALALIAIGSAFSYAPTFVVGTTGVTAD
                     WQGAASGLLNASQELGAAIGITVLAAIATAASVTAVDGHRTALIVAAGVLAAAQLLIR
                     QLPDAPD"
     gene            complement(1470..>2207)
                     /locus_tag="VM638_09320"
     CDS             complement(1470..>2207)
                     /locus_tag="VM638_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018806830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVM12656.1"
                     /translation="PMGRPETIDETARLVDEAGGTGIPVRVDHADPSQVRELIDRIVE
                     GRGRLDILVNDVWGGDPLTDWGASFWEHDLGNGIALVRNAVETHLITSWHAAPLLMRT
                     PGSLIVEVTDGVSDRYRGSLFYDLAKASVIRLAMAQAEELRTHGVTVLALTPGFLRSE
                     TVLEHFGVDEENWQAGIATDPNFAFSETPRYIGRAVASLAADPNKMRRTGTATSTWEL
                     ARAYGFDDVDGTRPDWGSRARELGISA"
BASE COUNT          363 a          736 c          807 g          301 t
ORIGIN      
        1 agcttgaggg gctagtccgg gaataccggg tgggggttca agtcccccct cgcccaccac
       61 gaagtcgcag gtcgctgcac acacggcgac cccggtggat cagtcggggg cgtccggtag
      121 ctgtcggatg agcagctgag cggcggcgag gacaccggcg gcgacaatga gcgcagtgcg
      181 gtgaccgtcg acggccgtga ccgatgccgc cgtggcgatc gcggcgagga cggtgatgcc
      241 gatggcggcg ccgagctcct gggatgcatt gaggagcccc gaggccgcgc cctgccagtc
      301 ggccgtgacg ccggtcgtgc ccacgacgaa ggtcggcgcg tacgagaagg cgctcccgat
      361 ggcgatcagc gcgagcgctg ggagcacgtc acgcagatag ctcccgtcgt cggggagtcg
      421 tgagagcagc gctgtcccca cggcggcgaa gccgaatccg atgctgagca gctggcgggc
      481 gccgaccgag gcgatccgcg ctccgatcct cggcgagatg aggagcacga gaagggtgat
      541 cggagcgaag gcggcgccca cagcgagggg cgagtagccg aggccgacct ggagatacag
      601 cgtcgtgaag aacaacgcgg cgccgagcgt ccccgcgttg acggcgagca ggacgttgcc
      661 ggtgacgacc tggcgcgttc tcaggagcgc gtggtcgacg aggggctcgt cgtgacgtcg
      721 ctcggaaacg gcgaacgccg ccagcagcgc gcaggcgacg cctgctccga cggcggtcgt
      781 ggtcgcggat gcaccctccc cgatccgagt gagtgtgagg gtcgccgctg cgagaccggc
      841 ggtgaggagg atcgcccccc ggctgtgcac ccgcgtcgct tcggcgggtc ggctctcctg
      901 gaggtggcgc gtggcgagcg cgatggtcgc gatcccgatt gggacgttga tcaggaagat
      961 cgaccgccac ccgaggacgt ccgtcagtgc tccgccgagg agctggccga caatcgctcc
     1021 gccggagccg acagcgcccc agtagccgag cgcgcggccg cgcgctccgt cctcttcgaa
     1081 ggtcaccatc agcagtgcga gcgcgcttgg cgaggccatc gcggcgccga ccccctggca
     1141 agctcgcgca gcgaccagcg accacccgga gtgcgcgagg ccggtcgcga gcgaagcgag
     1201 ggtgaagacc gccagcccgg cgaggaacag gcggcgacgt ccgaacacgt cacccatgcg
     1261 tccgaaccag atgagcaggc tgccgaacgt gagggtgtag gcgacggcga tgagctgcag
     1321 ccggctgtcc ggcagcccaa ggtcagcgcg gatcgacggg atggcgacgt tcaccacgac
     1381 gacatcgacg acaagcatga actgggcggc gcagagcaca cccagcgcgg cgacgcctcg
     1441 tcgctgtccg ctcagccgcg cggtcgccgt catgccgaga tccccagctc ccgagcgcgg
     1501 ctcccccagt ccgggcgcgt cccgtcgacg tcgtcgaagc cgtacgcccg ggccagctcc
     1561 cacgtcgagg tggcggtccc ggtgcggcgc atcttgttcg gatcggcggc gagcgaggca
     1621 acggctcgac cgatgtacct cggtgtctcg gagaatgcga agttcggatc cgtcgcgatg
     1681 cccgcctgcc agttctcctc gtcgactccg aagtgctcga gcaccgtttc agagcggagg
     1741 aacccgggtg tcagagccag gaccgtgacg ccgtgggtgc gcagctcctc ggcttgtgcc
     1801 atggcaagac ggatgaccga agccttggcg aggtcgtaga acaaggaccc gcggtatcgg
     1861 tcggagacgc catcggtcac ctccacgatc aacgatcccg gggttcgcat cagcagggga
     1921 gcggcgtgcc agctggtgat caggtgggtc tcgactgcgt tgcgtaccag cgcgatgccg
     1981 ttcccgaggt cgtgctccca gaacgacgcg ccccagtccg tgagcgggtc cccgccccac
     2041 acatcgttga cgaggatgtc gaggcgcccc cgaccctcga cgattcggtc gatcagctcg
     2101 cgcacctgcg aggggtccgc gtgatcgacc cgtaccggga tgcccgtgcc gcccgcctcg
     2161 tcgacgagtc gtgcggtctc atcgatcgtc tcggggcgtc ccatggg
//
LOCUS       DATLGG010000329         9799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_109852, whole genome shotgun sequence.
ACCESSION   DATLGG010000329 DATLGG010000000
VERSION     DATLGG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109852"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VM638_09325"
     CDS             <1..386
                     /locus_tag="VM638_09325"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HVM12657.1"
                     /translation="TDPAGLGPLEGPTRAAVGAMAARPYLVAGRDRLCTAVMEAIPRT
                     VVKIGAEGLVCAGLLDRGIGIALKVDDGSSRARAPALLAVLRDLDAATDEILDGPLAT
                     FAAPPVLGGGERVGRIAPAVELQRS"
     gene            413..895
                     /locus_tag="VM638_09330"
     CDS             413..895
                     /locus_tag="VM638_09330"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HVM12658.1"
                     /translation="MPDVDDAVATIDRFLRTAAEEDQLTLLGSGEGVWDVLVPSTWKE
                     SIAVTLRLGDWSLQGEAFFLRAPEDNRPESFHLLLQRNVRSGPWRFAANAAGDATLHA
                     YLPRAAISEDELDRLLGALVTLSDETYAPYMRLAYQRALEEQVRRGGPGVDQPPPWAR
                     "
     gene            948..1709
                     /locus_tag="VM638_09335"
     CDS             948..1709
                     /locus_tag="VM638_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVM12659.1"
                     /translation="MSHELIRRTRSAAAGALRRIRGSSPDEAERLEAALRLVLAASHD
                     FKTPLTGLYGWTRTLRYRWRTLPDAQREEVLDRMLRQLAGLSRTLDNVLATARLGTVP
                     AARPLDIRDAVHAAVREVLMLDPEREVVTVVPEHSVEVLADHARLGRILANLLDNAVK
                     YSPADAPVTIRLVEDGAEVRVEVEDRGPGLDRVEIREIFDPHVRTGVARRSGARGTGL
                     GLFLARSFAHDLGGDLLVRSRLGEGSTFALRLPRE"
     gene            2032..2571
                     /locus_tag="VM638_09340"
     CDS             2032..2571
                     /locus_tag="VM638_09340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVM12660.1"
                     /translation="MPGRVLIVVDTQEGFTRRGNLASEVTTAAIPAIRKLVEAEVDAG
                     TPLIFTKDSHVDGDPEFEMFPPHCIVGTEEHDLVEELRPFEGRATAVIEKMRYSAFFR
                     TGLEDLLRELDPDEVQVGGLCTDICVLHTVADLRNRDFRVAVHRDAVETFDAPGHDHD
                     EVNRWALGHIEGILGARVI"
     gene            2576..3502
                     /locus_tag="VM638_09345"
     CDS             2576..3502
                     /locus_tag="VM638_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HVM12661.1"
                     /translation="MGAPLLRDPVLWAARQPAVRRMAEEGGLARAIALRFVAGEDLDQ
                     AIVAARELNARGIGAMLDHLGENVASAEQASAAADGYVLAIKRIREDELPDANISVKL
                     TQLGLDVSRELCLENAGRVLEAAAGMQVMVDMEASPYVGATLEVVRELRERFLELGVC
                     VQAYLHRTAADVRRLAEEGVTVRVCKGAYLEPPSVAIPDRREVDRMFGRLAATLLAAG
                     CEVHLATHDLRLIEQAKRFAERRGIPPERFEFQMLHGIRRDLLQRLADDGYRTRTYVP
                     YGREWYPYLTRRLAERPANLWFFAANLLRWRR"
     gene            3499..4323
                     /gene="proC"
                     /locus_tag="VM638_09350"
     CDS             3499..4323
                     /gene="proC"
                     /locus_tag="VM638_09350"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HVM12662.1"
                     /translation="MTDRRVAFLGVGRMGEALVAGLIRAGGRASDEVMITARREERAK
                     ELADRYRVQATTSNAEACAWARTLVLTVKPQDMNTLLSQLEVTPEHLLVSFAAGIRTA
                     TLEAGTPEGVPVVRVMSNVPVLVDEALSVISAGRHATDEHLAVAEEILGNVGRVLRLR
                     EKHQDAVTATSGSGPAYFALLAEAMIDACILLGVSRDVATDLIVQTMLGTAKMLRDTG
                     MHPVEIREMVTSPGGTTIAAIRHLEQAGVRAAFLNAIEAARQRSAELAKGEDAEEV"
     gene            4338..4460
                     /locus_tag="VM638_09355"
     CDS             4338..4460
                     /locus_tag="VM638_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12663.1"
                     /translation="MGRGDRARTRWAKDRQRKKKATEKRQADERAAERKAARKR"
     gene            4473..5588
                     /locus_tag="VM638_09360"
     CDS             4473..5588
                     /locus_tag="VM638_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HVM12664.1"
                     /translation="MFPLSKGKVARQAHVGLPDGTFEEEHGREAFEGRASHLYRTHPP
                     TAWVRVDGPLQPKAFDLNGLKTPDQADPAAGWQRILWNDDLAIYVSRIAEAQPYYLRD
                     ADGDVCYFVHHGEGTLETDYGPLTYRAGDYLIVPKGTTHRLLPSGDDTFLYVIESAGE
                     YRIPEKGILGKHALFDPGVLEVPEPDPRDEQGEFTVRVKRDHDITTLTYPFHPLDVVG
                     WKGDLCPVKLNVADFRPVVSPRYHIPPSVHITWDSDGFEIGTFAPRPTETGDPDALRV
                     PFFHSNIDRDEVLFYHRGQFFSRAGIGEAFITHHPAGLHHGPQAAAIEASKTKEFADE
                     YAIMVETDRPLERDEALLSVEVEGYATSWAKGMGLLD"
     gene            5730..6716
                     /locus_tag="VM638_09365"
     CDS             5730..6716
                     /locus_tag="VM638_09365"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HVM12665.1"
                     /translation="METPQDPNEPTPQPTTPETPTEAAIPADPTLEPTAPAPAPAPPR
                     RRMLPWLIALAVFVAAGLGVGAWLLLSGPKKAATPEEALRGFYTAVAEDDCEAAASYL
                     DPSFQTQEQVCKDLGQIKEQVGTLGEIRSVDDQGNVAYVVASRTVEGQADQRIITVKK
                     GPEEGWLVAGGSACYGVEQPEDLGNDHLEEGETFDAYSSDPPTSGPHAPSPTETGQIY
                     SEPQPDAELVHAMEHGAVVFWMNFQDEALREQAQGAIDDVFTQGYESLIVTPKPDLDV
                     PFAMTAWGALQKCVGVDPQDIQTFVENHYGSGLEGSLACYGEASSLPGCNRS"
     gene            6780..7331
                     /locus_tag="VM638_09370"
     CDS             6780..7331
                     /locus_tag="VM638_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12666.1"
                     /translation="MKLRALVPAAALLLAACGGEDPTVGAPTTAPGPTVAPTTAAPAA
                     TGAVDAPPIEDAAALRDDLLRIAEENVTSEAGDALVPSFGVFEAWKQAYPELSFVGYA
                     IEPDERTVPTYSNLLNAKELEGPENPYIIAFAILDTQGTCAGGVMVGYPSPDTFEAVD
                     PDGEEQPCQPMALAAAGGYETFG"
     gene            7328..8278
                     /locus_tag="VM638_09375"
     CDS             7328..8278
                     /locus_tag="VM638_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVM12667.1"
                     /translation="MRRRRLGKQGPEISVVGYGAWEAGGEFWGPNESDQAVIDAIRAG
                     LDAGIDWIDTAEIYGQGTSEELVGRAVEGRRDEVVIATKVAPRPSGTGFRAEQVREAC
                     EGSLKRLGTDRIDLYQLHWPDARVPIEETWEAMVGLVEDGMVGAIGVSNFDQGLIERC
                     LAVRHVDSLQPHFSMLHPPNRELIAWCGEQGIGVVTYGPLAYGLLTGAFRSRDDFQPG
                     DWRRDGGASYYQAMFAPGKIERSFAVVEALRPIAERLEVTVAQLALAWNVHQPGVTSA
                     IAGSRNPKHIRQNAEAGDVDLDQEVLDELEQTLQLGPSFA"
     gene            complement(8292..9164)
                     /locus_tag="VM638_09380"
     CDS             complement(8292..9164)
                     /locus_tag="VM638_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12668.1"
                     /translation="MSVAQPSAASLLYLFADRSVEPDTLLSAGFAVPCTSSNVKRKDL
                     AAQLFAISFWSLQDQGKVTLDLGGRTLAGIGRRQVTASLVPGTVVRKPPARPSPLAAA
                     AASVQQMGPLPDLSQYQHLPGMEGMAEKMQEAAEQLAKLGISMPGQVPSEPVTPQPPA
                     DTGPPFILEDAILWQLQEAGGSAPVRDVIRSWFGRDEHEPWDSVIARVARELLEFGFG
                     DPRTPAPGGSPFGASSAPVSPDCERRAELEGAATELLDRWIAFLGTEDGLGETLLERC
                     GAAISSRRYDERAH"
     gene            9244..>9799
                     /locus_tag="VM638_09385"
     CDS             9244..>9799
                     /locus_tag="VM638_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVM12669.1"
                     /translation="MASDYQNHIGGKWVPASSGETFESRNPAQMDEVLGTFPRSGPDD
                     VEQAVQAAKAAYHDWMMTPAPTRSDYLMRVGLLLEQRMDEYANLMTREMGKTLKESRG
                     DIQEGIDFLYYMAGEGRRMFGHTVPSELRNKFSMTVRHPLGVVGLITPWNFPIAIPIW
                     KIAPAIVAGCTMVFKPAEDTPLLAT"
BASE COUNT         1472 a         3375 c         3572 g         1380 t
ORIGIN      
        1 tcacggaccc ggcgggtctc ggcccgctcg aaggaccgac gcgcgccgcc gtgggagcca
       61 tggcggcgcg gccgtacctc gtcgccggtc gcgaccgcct ctgcaccgcg gtgatggaag
      121 cgatccctcg cacggtggtg aagatcggtg ccgaagggct cgtgtgcgcc ggtctgctgg
      181 accgcgggat cgggatcgcg ctgaaggtcg acgacgggtc ctcgcgtgct cgtgcgcccg
      241 ccctcctcgc ggtcctgcgc gacctcgatg cggcgaccga cgagatcctc gacgggccgc
      301 tcgcgacgtt cgccgctccg ccggtcctcg gcggcggcga gcgcgtcggg aggatcgcgc
      361 ccgccgtcga gctccagcgg tcctgacggg gaccttccgg tagggtcgga gcgtgcccga
      421 tgtcgacgat gccgtcgcga cgatcgaccg cttcctgagg accgctgccg aggaggacca
      481 gctcacgctg ctcggctcgg gcgagggggt ctgggacgtc ctcgtcccct ccacctggaa
      541 ggagtcgatc gcggtgacgc tgcggctcgg ggactggagc ctccagggcg aggcgttctt
      601 ccttcgcgcg ccggaggaca atcggcccga gagcttccac ctcctgctcc agcgcaacgt
      661 ccgctccggg ccctggcgtt tcgcagcgaa cgcggccggg gacgcgaccc tgcatgccta
      721 cctgccgcgc gccgcgatct ccgaggacga gctcgaccgc ttgctcggcg ccctcgtcac
      781 gctctccgat gagacgtacg ccccctacat gcggctcgcg taccagcggg ccctcgagga
      841 gcaggtgcgg cgaggcggcc ccggggtgga ccagccgccg ccctgggcgc ggtaggcagg
      901 aacctcgcac ccgccgtcga acacgagcga ggaggttcct tcttcggatg tcgcacgagt
      961 tgatacgccg gacgcgctcg gccgccgctg gcgcgctgcg ccggatccgc gggtcctcgc
     1021 cggacgaggc cgagcggctc gaggctgcct tgcgcctggt cctggcggcc tcgcatgact
     1081 tcaagacgcc gctcaccggc ctctacggct ggacccgaac gcttcgctac cgctggcgga
     1141 cgctgcccga cgcgcagcgc gaggaggtcc tcgatcgcat gctccgacag ctcgcggggc
     1201 tgtcgaggac gctcgacaac gtcctcgcga cggctcgcct cggcacggtg ccggcggcgc
     1261 ggccgctcga catccgtgac gccgtccacg cggccgttcg cgaggtgttg atgctcgacc
     1321 ccgagcgcga ggtcgtcacc gtcgttccgg aacactcggt cgaggtcctg gcggatcacg
     1381 cccgcctcgg gaggatcctc gcgaacctgc tcgacaacgc ggtgaagtac tcgccggcgg
     1441 atgcgcccgt cacgatccgt ctcgtggaag acggcgcgga ggtccgcgtc gaggtcgagg
     1501 accggggtcc cgggctggac cgtgtcgaga tccgagagat cttcgaccca cacgtccgga
     1561 ccggggtggc gcgccgctcg ggggcgcgcg gcaccggcct cgggctgttc ctggcgcgtt
     1621 ccttcgccca cgatctcggc ggtgacctgc tggttcgctc gcgcctcgga gaaggatcga
     1681 cgttcgcgct ccgacttccg cgagagtgac aggaattttt ttctaggcca tctacctgcg
     1741 gaaacgcgtc gcgaacaggg attttggcag gttgacggct gcagcccctc cgcgtatcgt
     1801 tcgcgttgct ccgaccggct ggagcggcgg accggaagcc ccccggggcg gacggtcagc
     1861 caggaaggtc gatcggagca actggcccga ggggctgaag gtggtcggct cgatcggtgg
     1921 ggttcgcctc accggacggg acgacgagcc ggaggtccct cgggcctttc tcgtcgtccg
     1981 gcggatcggc gggatcgggc ccgcggatat actcggcgcg gaggtgagga gatgcccggc
     2041 cgcgtcctga tcgtcgtcga cacccaggaa gggttcaccc gccgcggcaa cctggcgagc
     2101 gaagtgacca cggcggccat cccagcgatc cgcaagctcg tggaggccga ggtggacgcc
     2161 gggacgcccc tcatcttcac gaaggacagc cacgtcgacg gcgaccccga gttcgagatg
     2221 ttcccgcccc actgcatcgt cgggaccgag gagcacgacc tcgtggagga gctccggccg
     2281 ttcgagggac gggcgacggc ggtgatcgag aagatgcgct actcggcgtt cttccggacc
     2341 ggcctcgagg atctgctgcg tgagctcgac cccgacgagg tgcaggtcgg tggcctctgc
     2401 accgacatct gcgtcctgca cacggtcgcg gacctccgca accgcgactt ccgcgtcgcg
     2461 gtccatcgcg atgccgtcga gaccttcgac gcacccggcc acgatcacga cgaggtcaac
     2521 cgctgggccc tcgggcacat cgaggggatc ctcggggcgc gggtgatctg aggagatggg
     2581 ggctccgctc ctccgcgacc cggtgctgtg ggcggcgcgt cagccggccg tccggcggat
     2641 ggcggaggag ggcgggctgg cgcgcgcgat cgcgctgcgg ttcgtcgccg gcgaggacct
     2701 cgaccaggcg atcgtcgccg cgcgcgagct gaacgcccgt gggatcggcg cgatgctcga
     2761 ccacctcggc gagaacgtgg cctccgccga gcaggcctcc gccgccgccg acggctacgt
     2821 gctcgcgatc aagcggatcc gcgaggacga gcttccggac gcgaacatct cggtgaagct
     2881 cacgcagctc gggctcgacg tctcgcgcga gctctgccta gagaacgccg ggcgggtcct
     2941 cgaggcggct gccggcatgc aggtcatggt ggacatggag gcgtccccct acgtcggggc
     3001 gaccctggag gtcgtgcggg agctgcgtga gcgcttcctc gagctcggcg tgtgcgtgca
     3061 ggcctacctg caccggaccg cggccgacgt gcggcgactg gccgaggagg gggtcacggt
     3121 ccgcgtctgc aagggcgcct acctcgagcc cccgtccgtc gcgatccctg accggcggga
     3181 ggtggaccgg atgttcggcc gcctcgcggc gacgctgctc gccgctggct gcgaggttca
     3241 cctcgccacc cacgacctcc gcctcatcga gcaggcgaag cggttcgcgg agcgccgcgg
     3301 gatcccgccg gagcggttcg agttccagat gctccacggg atccgtcgcg acctcttgca
     3361 gcggctggcc gacgacggct accgcacgcg gacctacgtc ccgtacggcc gtgagtggta
     3421 cccgtacctc acgcggcgcc tggcggagcg accggcgaac ctgtggttct tcgcggccaa
     3481 cctcctgcgg tggcgccggt gacggatcgt cgcgtcgcgt tcctcggcgt cgggcgcatg
     3541 ggtgaggcgc tcgtcgcggg gttgatccgc gcgggcggcc gggcgtcgga cgaggtcatg
     3601 atcaccgccc gccgcgagga acgggcgaag gagctggccg accgctaccg cgtgcaggcg
     3661 acgacgtcga acgccgaggc ctgcgcctgg gcgcgcacgc tcgtcctcac ggtcaagccg
     3721 caggacatga acacgctgct ctcgcagctc gaggtcaccc ccgagcatct gctcgtcagc
     3781 ttcgcggccg gcatccgcac cgcgacgctc gaggccggca cgcccgaggg cgtgccggtg
     3841 gtgagggtga tgtcgaacgt gccggtcctc gtcgacgagg cgttgtcggt gatcagcgcc
     3901 ggtcgccacg ccaccgacga gcatctggcg gtcgcggagg agatcctcgg caacgtcggg
     3961 cgggtcctgc gtctgcgcga gaagcaccag gacgccgtga ccgcgacctc cgggtcgggg
     4021 cccgcctact tcgcgctcct cgccgaagcg atgatcgacg cctgcatcct gctcggggtc
     4081 tcgcgcgacg tcgcgaccga cctgatcgtg cagacgatgc tcgggacggc gaagatgctc
     4141 cgggacacgg ggatgcaccc cgtcgagatc cgcgagatgg tgacctcgcc cggcgggacg
     4201 acgatcgcgg cgatccgcca tctggagcag gcgggtgtcc gggccgcgtt cctgaacgcg
     4261 atcgaggccg cgcggcagcg gagcgccgag ctcgcgaagg gtgaggacgc cgaagaggtc
     4321 tgagagagga gcacacgatg ggacgaggag acagggcacg gacgcgttgg gcgaaggatc
     4381 gccagcggaa gaagaaggcg accgagaagc ggcaggccga cgaacgagcg gccgagcgca
     4441 aggccgcgcg gaagcgctag gaggaggcga cgatgttccc gctgtcgaag ggcaaggtgg
     4501 cgaggcaggc ccacgtcggt ctgcccgacg gcacgttcga ggaggagcac gggcgcgagg
     4561 cgttcgaagg ccgcgcctcg cacctgtacc ggacgcaccc cccgaccgcg tgggtgcggg
     4621 tcgacgggcc gctccagccg aaggcgttcg acctgaacgg gttgaagacg ccggaccagg
     4681 cggaccccgc cgccggctgg cagcggatcc tgtggaacga cgacctggcg atctacgttt
     4741 cccggatcgc ggaggcgcag ccgtactacc tccgtgacgc ggacggcgac gtctgctact
     4801 tcgtccacca cggcgagggc acgctcgaaa ccgactacgg tccgttgacc taccgcgccg
     4861 gcgactacct catcgtcccg aagggcacga cgcaccgcct cctgccctcc ggcgacgaca
     4921 cgttcctcta cgtgatcgag agcgccggcg agtaccggat cccggagaag ggcatcctcg
     4981 gcaagcacgc gctcttcgac ccgggcgtcc tggaggttcc ggagcccgat ccgcgggacg
     5041 agcagggaga gttcacggtc cgcgtcaagc gggaccacga catcacgacc ctcacgtacc
     5101 cgttccaccc cctcgacgtc gtcgggtgga agggcgacct gtgcccggtg aagctcaacg
     5161 tcgccgactt ccgcccggtc gtctcgccgc ggtaccacat cccgccgagc gtgcacatca
     5221 cgtgggacag cgatgggttc gagatcggga ccttcgcacc ccggccgacc gagacgggcg
     5281 accccgacgc gctgcgggtg cccttcttcc actcgaacat cgaccgggac gaggtgctct
     5341 tctaccaccg cggccagttc ttctcgcggg cggggatcgg tgaggcgttc atcacgcacc
     5401 acccggccgg gctgcaccac ggtccccagg cggcggcgat cgaggcctcg aagacgaagg
     5461 agttcgcgga cgagtacgcg atcatggtcg agaccgatcg cccgctcgaa cgcgacgagg
     5521 cgctgctgtc ggtcgaggtc gagggctacg ccacctcctg ggcgaagggc atgggtctgc
     5581 tggactgagc catccgccag ccgcaccacc cgagtggttg gtcggctggc tagcccgctc
     5641 gggccatctt cgggtggatt ccgccgctcg gtggtgacct cgcccggcgc gtccactacc
     5701 gtcggctcac gccatcgaga ggagcggatg tggagacacc gcaggacccg aacgagccga
     5761 cgccgcagcc gaccacaccg gagacaccca ccgaggccgc gatcccggcc gacccgacgc
     5821 tggagccgac ggctccagcc ccggcgccgg cgccgccccg tcggcggatg ctgccgtggc
     5881 tgatcgcgct cgcggtgttc gtcgcggccg gcctcggggt cggtgcgtgg ttgctcctgt
     5941 ccggtccgaa gaaggcagcg accccggagg aggcgctgcg cggcttctac acggcggtgg
     6001 ctgaagacga ctgcgaggcc gcggcctcct acctcgaccc ttccttccag acgcaggagc
     6061 aggtctgcaa ggacctcggt cagatcaagg agcaggtcgg cacgctcggc gagatccggt
     6121 cggtcgacga ccaggggaac gtggcgtacg tggtcgcctc ccgaacggtc gagggccagg
     6181 cggatcagcg gatcatcacg gtgaagaagg ggccggagga gggctggctc gtggccggtg
     6241 ggtccgcgtg ctacggggtc gagcagccgg aggacctcgg caacgaccac ctcgaggagg
     6301 gggagacctt cgacgcgtac agctcggatc ctccgacgtc ggggccgcac gcgccgagcc
     6361 ccacggagac cgggcagatc tacagcgagc cgcagccgga tgccgagctg gtgcacgcga
     6421 tggagcacgg cgccgtggtc ttctggatga acttccagga cgaggcgctg cgtgagcagg
     6481 cgcagggcgc gatcgacgac gtcttcacgc agggctacga gtcgctgatc gtgacgccga
     6541 agcccgacct ggacgtcccg ttcgcgatga ccgcctgggg tgcgctccag aagtgcgtcg
     6601 gcgtcgaccc gcaggacatc cagacgttcg tcgagaacca ctacggctcg gggctcgagg
     6661 gatccctcgc ctgttacggc gaggcctcgt cgctcccggg ctgcaaccgt agctgagcgc
     6721 gcgtttcgcg ggtgggccgg ggggaaggtc agtcgcgtgg atccgaacgg aggatgtgga
     6781 tgaagctgag agctctcgtt cccgccgcgg ccctgctgct cgccgcgtgc ggcggggaag
     6841 acccgacggt cggcgcgccg acgaccgcac caggtcccac cgtggcgccg accacggccg
     6901 ctcccgcggc gaccggcgcc gtcgacgcgc cgccgatcga ggacgcggcg gccctccggg
     6961 acgacctcct gcggatcgcc gaggagaacg tgacgtcgga ggcaggtgac gctctggtgc
     7021 cgtcgttcgg cgtcttcgag gcgtggaagc aggcctatcc ggagctgtcc ttcgtcggct
     7081 acgcgatcga acccgacgaa cgcacggtcc cgacctattc gaacctcctg aacgcgaaag
     7141 agctggaggg gccggagaac ccctacatca tcgcgttcgc gatcctcgac acgcagggga
     7201 cgtgtgccgg cggcgtcatg gtcggctacc cctcaccgga cacgttcgaa gcggtcgacc
     7261 ccgacggcga ggagcagccg tgccagccca tggcgctcgc cgcggccggc ggctacgaga
     7321 cgttcggatg aggaggcgca ggctcggcaa gcaggggccc gagatctcgg tcgtcgggta
     7381 cggggcgtgg gaggccggcg gggagttctg gggaccgaac gagtccgacc aggcggtgat
     7441 cgacgcgatc cgggccggcc tcgacgcggg catcgactgg atcgataccg cggagatcta
     7501 cggacagggc acctcggagg agctcgtcgg ccgggcggtc gaggggaggc gggacgaggt
     7561 cgtgatcgcg acgaaggtcg cgccgcgccc ttcgggaacg gggttccggg cggagcaggt
     7621 gcgtgaggcc tgcgagggga gcctcaagcg cctggggacc gaccggatcg acctgtatca
     7681 gctccactgg cccgatgcgc gcgtccccat cgaggagacc tgggaggcga tggtcgggct
     7741 cgtcgaggac ggcatggtcg gcgccatcgg ggtgtcgaac ttcgaccagg gtctgatcga
     7801 gcggtgcctc gcggtccgcc acgtcgattc cctccagccc cacttctcga tgctccatcc
     7861 gccgaaccgc gagctgatcg cctggtgcgg cgagcagggg atcggggtcg tgacctacgg
     7921 gccgctcgcc tacgggctgc tcacgggcgc gttccgctcg cgcgacgact tccaaccggg
     7981 ggactggcgc cgcgacggcg gcgcctccta ctaccaggcg atgttcgccc ccggcaagat
     8041 cgaacggagc ttcgcggtcg tcgaggcgct gcgtcccatc gccgagcggc tcgaggtgac
     8101 cgtcgcccag ctcgccctcg cgtggaacgt ccaccagccc ggcgtgacct ctgcgatcgc
     8161 cggcagccgc aacccgaagc acatccgcca gaacgccgag gccggcgacg tggacctcga
     8221 ccaggaggtc ctcgacgagc tcgagcagac cctccagctc gggcccagct tcgcgtagct
     8281 cgctcgacgc ttcagtgagc ccgctcgtcg tagcggcggg aggagatggc ggcgccgcag
     8341 cgttcgagga gggtctcgcc gaggccgtct tcggttccga ggaaggcgat ccagcggtcc
     8401 agcagctccg tcgcggcgcc ttccagctcc gcgcgacgct cgcagtcggg ggagaccggt
     8461 gccgacgagg cgccgaacgg cgagccgccc ggcgcgggcg ttcgagggtc gccgaagccg
     8521 aactcgagca gctcgcgggc cacgcgggcg atcaccgagt cccacggctc gtgctcgtca
     8581 cggccgaacc agctcctgat cacgtcgcgc acgggagcgc ttccgccggc ttcctgaagc
     8641 tgccacagga tcgcgtcctc gaggatgaac ggcggccccg tgtccgcggg cggttgcggc
     8701 gtcaccggct cgctcgggac ctgtccgggc atcgagatcc cgagcttggc gagctgctcg
     8761 gcggcctcct gcatcttctc ggccatcccc tccatccccg ggaggtgctg gtactgcgac
     8821 agatccggga gcgggcccat ctgctggacg ctcgcggccg ccgcggcgag cggagaggga
     8881 cgcgccggcg gcttgcggac gacggtcccg ggtacgaggg aggccgtgac ctgccgccgc
     8941 ccgatccccg cgagcgtgcg gccaccgagg tcgagcgtga ccttcccctg gtcctgcagg
     9001 ctccagaagg agatggcgaa gagctgggcc gccaggtcct tgcgcttcac gttcgacgag
     9061 gtgcacggga ccgcgaagcc tgcgctgagc agcgtgtccg gttcgaccga tcggtcggcg
     9121 aacaggtaca gcagcgacgc agcgctcggc tgggcgaccg acatggccgg gaggataccg
     9181 ggggtgtccc gcggcggcca tccgcgcccg agtaggattg aggcgacgat cgaggaggaa
     9241 gcgatggcga gcgactatca gaaccacatc ggcggcaagt gggtcccggc ttcgtcgggc
     9301 gagacgttcg agtcgaggaa cccggcccag atggacgagg tgctgggcac cttcccgcgg
     9361 agcggtccgg atgacgtcga gcaggccgtg caggcggcga aggcggcgta ccacgactgg
     9421 atgatgacgc cggcgccgac gcgctcggac tacctgatgc gggtggggct cctcctcgag
     9481 cagcgtatgg acgagtacgc gaacctcatg acccgcgaga tgggcaagac cctgaaggag
     9541 agccgcggcg acatccagga gggcatcgac ttcctctact acatggcggg cgaaggccgg
     9601 cggatgttcg gccacaccgt cccctccgag ctccggaaca agttctcgat gacggtccgt
     9661 caccctctcg gcgtggtcgg gctcatcacg ccgtggaact tcccgatcgc gatcccgatc
     9721 tggaagatcg cgccggcgat cgtggccggc tgcacgatgg tgttcaagcc cgccgaggac
     9781 acgcccctgc tcgcgacgc
//
LOCUS       DATLGG010000330         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_110013, whole genome shotgun sequence.
ACCESSION   DATLGG010000330 DATLGG010000000
VERSION     DATLGG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110013"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VM638_09390"
     CDS             complement(<1..192)
                     /locus_tag="VM638_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12670.1"
                     /translation="MSRAPDVRAGIARLQNGAHELFIIAAVMPLFLAGLELAATGFSD
                     AGFTLAERGARAAFLDRVLY"
     gene            complement(199..414)
                     /locus_tag="VM638_09395"
     CDS             complement(199..414)
                     /locus_tag="VM638_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HVM12671.1"
                     /translation="MAHEIKSPMVGKVFRIECAVGQTINEGDVVIVLESMKMEMPVEA
                     DASGVVQSIPVQVEQTVQEGDVLVVLG"
     gene            complement(428..1291)
                     /locus_tag="VM638_09400"
     CDS             complement(428..1291)
                     /locus_tag="VM638_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVM12672.1"
                     /translation="MKTIISCALSGVAANRAQCPGIPYTPEEYAAEAKRAADEGCSIV
                     HIHARKPDGIPSQDVEDYRAIRDAIVAKVPNLIINFSTGAIGYTKEQRIASVPACKPD
                     IAALNMGTMNYAKYSEKRKDFVFKLVFENSFDDIIFYLKTMKEHGVRPEMECFDVGHV
                     GSAYPLIDMGVLEPPYEFSFILGVDGGVPATTKNIITMMDNLPPSSNWQVIGISRDQW
                     RLCAAALTLGGHIRVGFEDNFYLPEGTMAKSNGELVARAARLSRDVGREVASIDEARQ
                     MLHLPLKRAAA"
     gene            complement(1293..2117)
                     /locus_tag="VM638_09405"
     CDS             complement(1293..2117)
                     /locus_tag="VM638_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVM12673.1"
                     /translation="MRVNPGWDRRPMAYETLLYEVKDRIARITINRPDKRNALNATVI
                     AELVAAFRAADADANVGAIVLTGAGEKAFCAGADLGGGGMIGAQTVYERHEAGRNFLD
                     LFRTMRSIGKPIVCAANGHVLAGGLGLALACDALIAVDDADFGTPEINVGLFPYVIMA
                     TIVRSSTNRKRVLELLLTGDKIRGAEVERLGWANRVVPRAELAAAVDELAKKLASKSP
                     AILRLGRRAWYAMGDMTFEQAIEYLQACLTVNTLTEDAAEGIGAFLEKREAKWKGR"
BASE COUNT          322 a          756 c          747 g          366 t
ORIGIN      
        1 gtagagcacg cggtcgagga acgccgcgcg cgcgccgcgc tcggcgagcg tgaagccggc
       61 gtcggagaag ccggtggccg cgagctcgag cccggcgagg aagagcggca tcacggccgc
      121 gatgatgaag agctcgtgcg cgccgttctg caggcgcgcg atgccggccc gcacgtcggg
      181 cgcgcggctc atcggaccct agcccaggac cacgagcacg tccccctcct gcacggtctg
      241 ctcgacctgc acggggatcg actgcacgac gccggaggca tccgcctcga cgggcatctc
      301 catcttcatc gactcgagca cgatcacgac gtccccttcg ttgatcgtct gcccgaccgc
      361 gcactcgatg cggaacacct tgccgaccat cggggacttg atctcgtgag ccatgtcgcg
      421 cgggagatta cgcggcagcg cgcttcagag gcaaatgaag catctgccgc gcctcgtcga
      481 tcgaggcgac ctcgcgcccg acgtcgcggg agaggcgggc ggcgcgggcg acgagctcgc
      541 cgttcgactt cgccatcgtc ccctccggca ggtagaagtt gtcctcgaag ccgacccgga
      601 tgtggccgcc gagggtgagc gcggcggcgc agagcctcca ctggtcgcgg ctgatgccga
      661 tcacctgcca gttcgagctc ggcggcaggt tgtccatcat ggtgatgatg ttcttcgtcg
      721 tcgccgggac gccgccgtcc acgccgagga tgaagctgaa ctcgtagggc ggttcgagga
      781 cgcccatgtc gatgagcgga taggcgctgc cgacgtgacc gacgtcgaag cactccatct
      841 ccgggcggac gccgtgctcc ttcatcgtct tcaggtagaa gatgatgtcg tcgaaggagt
      901 tctcgaagac gagcttgaag acgaagtcct tccgcttctc ggagtacttc gcgtagttca
      961 tcgtccccat gttgagcgcc gcgatgtccg gcttgcacgc ggggacgctc gcgatccgct
     1021 gctccttcgt gtagccgatc gcacccgtcg agaagttgat gatgaggttc gggaccttcg
     1081 cgacgatcgc gtcgcggatc gcccggtagt cctcgacgtc ctgggacggg atgccgtcgg
     1141 gcttccgcgc gtggatgtgg acgatcgagc agccctcgtc cgcggcgcgc ttcgcctccg
     1201 cggcgtactc ctcgggcgtg taggggatgc cggggcactg cgcccggttg gcggcgacgc
     1261 cggagagcgc gcacgagatg atcgtcttca tcctatctgc ccttccattt cgcttcgcgc
     1321 ttctcgagga acgcgccgat cccctccgcg gcgtcctcgg tcagcgtgtt cacggtgagg
     1381 caggcctgca ggtactcgat cgcctgctcg aaggtcatgt cccccatcgc gtaccaggcg
     1441 cggcggccca ggcggaggat cgccgggctc ttcgaggcga gcttcttcgc cagctcgtcc
     1501 accgcggcgg cgagctccgc gcgcggcacg acccggttcg cccacccgag ccgctcgacc
     1561 tcggcgcccc ggatcttgtc cccggtaagg aggagctcga gcacccgctt ccggttcgtc
     1621 gacgagcgga cgatcgtcgc catgatcacg taggggaaga gcccgacgtt gatctcgggc
     1681 gtgccgaagt ccgcgtcgtc gacggcgatg agtgcgtcgc acgcgagggc cagccccagc
     1741 cctccggcga gcacgtgccc gttcgccgcg cacacgatcg gcttcccgat cgaccgcatc
     1801 gtgcggaaga ggtcgaggaa gttgcggccg gcctcgtgcc gctcgtacac cgtctgcgcg
     1861 ccgatcatcc cgccgccccc gaggtcggcg cccgcgcaga acgccttctc gccggcgccg
     1921 gtgaggacga tcgcgccgac gttcgcgtcc gcgtccgccg cgcggaacgc cgcgacgagc
     1981 tcggcgatga ccgtcgcgtt gagcgcgttg cgcttgtcgg gccggttgat cgtgatccgc
     2041 gcgatgcggt ccttcacctc gtagaggagc gtctcgtagg ccatcgggcg gcgatcccag
     2101 cccggattga cacgcatgtc aacatacggt ccgcgccggt cggacgccga ccggttggaa
     2161 ctctgccgga cgggcagcca cgcgtatgga c
//
LOCUS       DATLGG010000331         3173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_187975, whole genome shotgun sequence.
ACCESSION   DATLGG010000331 DATLGG010000000
VERSION     DATLGG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187975"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..585
                     /locus_tag="VM638_09410"
     CDS             172..585
                     /locus_tag="VM638_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVM12674.1"
                     /translation="MGAHASITTRRRVQWADTDASGWYHNTVVLRWIEETENELMDRI
                     GILHDIISRVPRVHIEVDYRKILRFWEEVDVTVEVEQVGGASVRFRFEVRAGDEVAAE
                     GWYAAVLVDDAGKPLPWSEGHRRALLSPVEGRVGG"
     gene            582..1391
                     /locus_tag="VM638_09415"
     CDS             582..1391
                     /locus_tag="VM638_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HVM12675.1"
                     /translation="MTDHAPQVRRMGRVSEDEEGRLSYGDYLRLDELLDLQSPLSDPP
                     AHDELLFIIVHQAYELWFRELVFELESVRDLIREGQIERARHFLTRVHAIERLMIEQI
                     EVLETMDPQDFLEFRSHLDPASGFQSVQFREIELLSGLKEPQYVRMTPPRERPRLERR
                     LEEPTLWDALRELMEQHGLPMPEDDEEVRRASLAKMALDRDAFPGLWYLADDLIKHDE
                     LFTLWRQRHVLMVERQIGFKTGTGGSSGASYLRTTLEKRFYPELWELRSQL"
     gene            1460..1975
                     /locus_tag="VM638_09420"
     CDS             1460..1975
                     /locus_tag="VM638_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12676.1"
                     /translation="MMLFVSIGGFFFVDTAAQPLGSPEQGGGGTRVAVVEGVEIELLP
                     GWQEAERFTDPPGVRLTRGIGSLDAFAIDFAGTPDALFESYFVEALEPQSSQLQRPVT
                     VEPLSIEGASAVRGPYTGSFGDLGPVEGEVTALVTPEGTGVVFDGWAHQGLYAQIAEE
                     VRVMVEGVGLG"
     gene            1968..3170
                     /locus_tag="VM638_09425"
     CDS             1968..3170
                     /locus_tag="VM638_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HVM12677.1"
                     /translation="MAEAPTRHPRWGHRTSLFQWHQPALYVYGLILLLTGLITIAEQG
                     LYQDMSPSGWALSWMLMVIYGVPVFLLIYWLDLYEREPLSLLFGALLWGGVAAVTMSG
                     IANMGWGLVLTQAAGPEFAARWAPALTAPWTEEILKASGVILIYLIARREIDDVMDGF
                     VYGAVVGLGFAIVEDVFYFMAVFGGQTEGVLFGFFIRVIASGLYGHVLYTGLAGMGIA
                     YFVSRHGERSFGNRLAVALGLFVVAVFGHFLWNSPLLDFFPEPPFEPLDYLQIMGATA
                     VKGVPLLVFAALMIVLAHRREHRWLEAALADEVGREGLTAEEWRDLGTTKSRRRAVRE
                     AKGRGGPVAARQLKRLHRQQVNLAMVRTRVDDPEHPDLVRQRAYCAALREALRSLPGM
                     HRDPVEAS"
BASE COUNT          429 a         1057 c         1167 g          520 t
ORIGIN      
        1 ggtcgagggc gccgtagccg gtccgctcga tcacgccgag gctcgtgaac ggcccggcct
       61 ccgctcgccg cgcccaggcc ggcagcagcg acgggtcggt gccggggatc ccgttcggca
      121 gcccgatccc gacgcgcatc ggcgaggagc ctaccgtgcg atgatgccgc cgtgggagcg
      181 cacgcctcga tcaccacgcg ccgccgcgtg cagtgggcgg acaccgacgc ttcgggctgg
      241 taccacaaca cggtcgtcct gcggtggatc gaggagaccg agaacgagct gatggaccgg
      301 atcgggatcc tccacgacat catctctcgg gtgcctcggg tccacatcga ggtcgactac
      361 cggaagatcc tgcggttctg ggaggaggtt gacgtcacgg tcgaggtgga gcaggtaggc
      421 ggcgcctcgg tccgcttccg gttcgaggtt cgcgcgggcg acgaggtcgc cgcggagggg
      481 tggtacgcgg ccgtgctcgt cgatgacgcg ggcaagcccc tgccctggtc cgaagggcat
      541 cgccgggcgc tgctctcccc ggtcgagggg agggttggag gatgaccgac cacgcaccgc
      601 aggtccgccg gatgggccgc gtctcggagg acgaagaggg acgcctctcc tacggcgact
      661 accttcggct cgacgagctc ctcgacctcc agtcgcccct gtcggatccg ccggcgcacg
      721 acgagctcct cttcatcatc gtccaccagg cgtacgagct ctggttccgg gagctggtgt
      781 tcgagctcga gtcggtccgt gacctgatcc gcgaagggca gatcgagcgg gcgcgccact
      841 tcctcacgcg cgtccacgcg atcgagcggc tcatgatcga gcagatcgag gtgctcgaga
      901 cgatggaccc gcaggacttc ctcgagttcc gttctcacct cgacccggcg agcgggttcc
      961 agtcggtcca gttccgggag atcgagctcc tgtcgggact caaggagccg cagtacgtgc
     1021 ggatgacccc gccgcgcgag cggccgcgcc tcgagcggcg gctggaggag ccgaccctgt
     1081 gggacgcgct gcgcgagctg atggagcagc acggcctgcc gatgccggag gacgacgagg
     1141 aggtgcggcg ggcgagcctc gcgaagatgg cgctggaccg cgacgcgttc ccggggctct
     1201 ggtacctggc ggacgatctg atcaagcacg acgagctgtt cacgctctgg cgccagcggc
     1261 acgtcctgat ggtcgagcgc cagatcgggt tcaagaccgg gacgggtggc tcgagcgggg
     1321 cgagctactt gcggacgacg ctcgagaagc ggttctatcc ggagctgtgg gagctcagga
     1381 gccagctgta ggcgctccgc ccccgccgcc ccccggcccg aaggagcggc ggtggctgcc
     1441 gccgttcctc ctcctcgggg tgatgctgtt cgtctcgatc ggcgggttct tcttcgtcga
     1501 caccgcggcg cagccgctcg gctccccgga gcagggcggc ggcggaacgc gggtcgcggt
     1561 ggtcgagggg gtcgagatcg agctcctgcc gggctggcag gaggccgagc ggttcaccga
     1621 tccgccgggc gtgcgcctga cgcgcggcat cggctcgctc gacgcgttcg cgatcgactt
     1681 cgccgggacg ccggacgcgc tcttcgagag ctacttcgtc gaggcgctcg agccacagtc
     1741 gagccagctc cagcggcccg tgacggtcga accactgtcg atcgagggag cgtccgcggt
     1801 gcgtggtccc tacacgggat ccttcggcga cctcggtccc gtcgagggcg aggtgacggc
     1861 gctcgtgacg ccggagggca ccggcgtcgt cttcgacggg tgggcgcatc aggggctgta
     1921 cgcgcagatc gcggaggagg tccgggtcat ggtcgagggg gtgggccttg gctgaggctc
     1981 cgacccgcca ccctcggtgg ggccaccgca cctcgctgtt ccagtggcat cagcccgcgc
     2041 tctacgtcta cgggctgatc ctgctcctca ccggactgat cacgatcgcg gagcaggggc
     2101 tctaccagga catgtccccg tccggctggg ccctgtcgtg gatgctcatg gtgatctacg
     2161 gcgtcccggt cttcctgctg atctactggc tcgacctcta cgaacgcgag cccttgtcgc
     2221 tgctgttcgg cgcgctcctg tgggggggcg tggcggcggt gacgatgtcc gggatcgcca
     2281 acatgggctg gggcctcgtc ctcacgcagg cggcgggccc cgagttcgcg gctcgctggg
     2341 cccccgcgct caccgcgccc tggaccgagg agatccttaa ggcgagcggc gtcatcctga
     2401 tctacctcat cgcgcgtcgc gagatcgacg acgtgatgga tgggttcgtc tacggcgccg
     2461 tcgtcggcct cggcttcgcg atcgtcgagg acgtcttcta cttcatggcc gtcttcgggg
     2521 gccagaccga aggcgtgttg ttcgggttct tcatccgcgt gatcgccagc gggctgtacg
     2581 ggcacgtgct ctacacgggc ctcgccggca tggggatcgc gtacttcgtg agccgccacg
     2641 gggaacgatc gttcgggaac cgactcgcgg tggcgctcgg cctgttcgtg gtcgctgtct
     2701 tcggccattt cctgtggaac tcaccgctgc tcgacttctt ccccgagccg ccgttcgagc
     2761 cgctcgacta cctccagatc atgggcgcga ccgcggtgaa gggcgtgccg ctgctcgtct
     2821 tcgccgcgct gatgatcgtg ctggcgcacc ggcgggagca ccgctggctc gaggctgccc
     2881 tggcggacga ggtcgggcgc gaggggctga cggccgagga gtggcgagat ctcgggacga
     2941 cgaagagccg ccgccgggcc gtgcgtgagg cgaaggggcg gggcggtccg gtcgcggcgc
     3001 gccagctcaa gcgtctccac cgccagcagg tgaacctcgc gatggttcgc acgcgcgtgg
     3061 acgatcccga gcatccggac ctcgtccgcc agcgcgccta ctgcgcggcg ctgcgggagg
     3121 cgctgcgttc gctcccgggc atgcaccgag acccggtgga ggcgtcgtga agg
//
LOCUS       DATLGG010000332         6143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_188029, whole genome shotgun sequence.
ACCESSION   DATLGG010000332 DATLGG010000000
VERSION     DATLGG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188029"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VM638_09430"
     CDS             complement(<1..321)
                     /locus_tag="VM638_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HVM12678.1"
                     /translation="MAEVFRSAIPEDTALTEAEHALARGDLVVVPTDTVYGLAAQPNV
                     PGATQRVFEVKGRSRDLILPVLVADVDDAAEIAVLDERARTLAADFWPGGLTLIVPRT
                     AASEG"
     gene            complement(314..1147)
                     /gene="prmC"
                     /locus_tag="VM638_09435"
     CDS             complement(314..1147)
                     /gene="prmC"
                     /locus_tag="VM638_09435"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVM12679.1"
                     /translation="MRPAEVVDRAADYLERHGVESPRATAEALLLYFLQTDRAGLYVR
                     REGLDTRTAKLFGRALCQRCHGVPLQYLTGEQQFFDLVLGVAPGVFVPRPETEVVVER
                     ALEVIEQVEVPVVVDVGTGTGAIALAVKRRRADAAVIAIDRSPDAVAAAGANASRHAL
                     EVEVLEGDLLDPVPERLRGRVDLLVSNPPYVTREEYEDLPDEVKAEPYEALVGGTEVH
                     ARLAAEAPRWLAPGGWLVMEIGETQGDEARVLLEGAGFDEIEVLPDLAGRDRVVRGRR
                     G"
     gene            1348..2457
                     /locus_tag="VM638_09440"
     CDS             1348..2457
                     /locus_tag="VM638_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HVM12680.1"
                     /translation="MVVLHEGADAEEVARGHAAAHGATVDHVYRHALRGYAARMSGAA
                     ARRVASDARVRSVTPDRVVRASAQTLPTGIDRVDAEASSTQAGNGSGSVGVDVAVLDS
                     GIDTKHRDLNVVGGKNCSTGKSYADGNGHGTHVAGTIAAKDDANGVVGVAPGARLWAV
                     RVLNNSGSGSWSSVICGVDWVTANKGTIEVANMSLGGSGSEPAATGCSTGDALHDAIC
                     RSVAAGVTYVVAAGNESKDSKNAVPAAYDEVITVSALADFDGNPGGLAAPTCRTDEDD
                     TFGSFSNFGADVDLIAPGVCILSTWKGGGYDTISGTSMASPHVAGGAALYKAANPGAS
                     PAQVQTALVAAGGTDWNNTDDRDAIKEPLLNVSSF"
     gene            complement(2468..3535)
                     /gene="prfA"
                     /locus_tag="VM638_09445"
     CDS             complement(2468..3535)
                     /gene="prfA"
                     /locus_tag="VM638_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVM12681.1"
                     /translation="MRDRLEEIEAAYEELNQELSSPEVASDPTKLRDLGKRHRELGEI
                     VAASRAWRKATADAEEARALAADESDPESRAFYEQEADRAAAQAEELRAQIEVLLLPR
                     DPNDEKNVVVEIRAGEGGEEAALWAGDLLEMYLKYAERRRWKTDVLASSPSSLGGYDK
                     VILEIRGDGAYSRLKHESGVHRVQRVPQTESQGRIHTSAASVAVLPEAEEVDVEIRPE
                     DIEVDVFRSSGPGGQSVNTTDSAVRLTHKPTGIVVSVQEERSQLQNRDKAMRYLRARL
                     LQMEQERQQQEQAEARRAQVGGGGRAEKIRTYNFPESRVTDHRINLKVHRLEQVLQGE
                     LDEFIDALLAEERAKQLRAGG"
     gene            complement(3540..4499)
                     /locus_tag="VM638_09450"
     CDS             complement(3540..4499)
                     /locus_tag="VM638_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HVM12682.1"
                     /translation="MPQKEHYYGGQAVIEGVMMRGRDLWAIAVRKPDGGIHTEAHEID
                     SVAKRVKILAKPGLRGVIALGQALAIGVKALTISANHSVEEEEKLTPRQMALSMTFAM
                     VLFIGVFIVLPAFLSNLAKDRVASGVWLNVGEGIFRVLLFLGYLWAIGRMRDIRRVFQ
                     YHGAEHKTIAAYEHDDDWSDPERVDRYSTLHVRCGTNFLLIVMVLTIFVFALFGTPGL
                     AWRIGSRIIAIPLIAGLAYEALRLGARYSESRFMRALMTPGLWLQKITTKPPTPDQIE
                     VAITSFGEVIRREQEAGAGAEAESPAPPVEGAAAPVEPDDGGR"
     gene            complement(4533..5897)
                     /locus_tag="VM638_09455"
     CDS             complement(4533..5897)
                     /locus_tag="VM638_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12683.1"
                     /translation="MKRIVVAMAPLLVLLPAAPGAVAGKRIDGLPANVVSQAFPAAVR
                     TYPVLDRTGQAIGTTRWRVVRNTGNCCENHFAATADGRLLDFGGTYIHFTDDKGATWR
                     RVAPIEPFMGGEGTITVAANGDVLGVAWDVYSGDRILGFKWDAATKLWTYQNNVLHTP
                     FYDREWVAVVERPGILPGRPMKNPYMSILKGGWPSKDVWSYSFDGVHYTLATNKRATG
                     VGQETVSRWLPTTRQAAADWTQSITASSITPLPGGGALARPADTLDSLFGGGWMILEP
                     GTLRWSPFRLGDGSVLPPGRLLIDSNGHAHLLNASGGTVTYRLSRDGGRTWDTTTASL
                     PAGSTVEEWDAKASGALGRAIVGVHAHRSSTNTDQDYVLEFATGCGAPQHLMTHVAGA
                     GNLNAGSGVGASIRFDFATVALLPDGTIVTSLQDQSQPQPSAAIALETTPNDPEAELP
                     QPSC"
BASE COUNT          862 a         2222 c         2167 g          892 t
ORIGIN      
        1 cccctccgaa gccgcggtcc tcggcacgat cagcgtgagg ccgccgggcc agaaatcggc
       61 ggccagcgtg cgggcccgct cgtcgaggac ggcgatctcg gcggcgtcgt cgacgtccgc
      121 gaccagcacc ggcaggatca ggtcgcgcga gcggcccttg acctcgaaga cgcgctgcgt
      181 ggcgccgggg acgttcggct gcgcggcgag gccgtacacg gtgtcggtcg gcacgacgac
      241 gaggtccccg cgagcgagcg cgtgttccgc ctcggtcagc gcggtgtcct cggggatcgc
      301 ggagcggaag acctcagcca cgacgacccc gcacgacgcg gtcccgcccc gccaggtccg
      361 gcaggacctc gatctcgtcg aagcccgcgc cctcgagcag cacccgcgct tcgtccccct
      421 gggtctcccc gatctccatc acgagccacc cgccgggcgc gagccatctc ggagcctcgg
      481 cggcgaggcg cgcgtggacc tcggtcccgc cgacgagcgc ctcgtagggc tcggccttca
      541 cctcgtccgg gaggtcctcg tactcctcgc gcgtgacgta cggggggttg ctcacgagca
      601 gatccacccg ccctcgcagc cgctccggaa cggggtcgag gaggtcaccc tcgaggacct
      661 ccacctcgag cgcgtgccgc gatgcgttcg cgccggccgc cgccacggcg tcgggggaac
      721 gatcgatggc gatgaccgcc gcgtccgcgc ggcggcgctt caccgccaga gcgatcgcgc
      781 cggtgccggt cccgacatcg accaccaccg gcacctcgac ctgttcgatg acctcgagcg
      841 cccgctccac caccacctcc gtctccggac ggggaacgaa tacccccggg gctaccccca
      901 gaaccaggtc gaagaactgc tgctcaccgg tgaggtactg gagcgggacg ccgtggcagc
      961 gctggcagag ggcgcggccg aagagcttcg cggtccgcgt gtccagcccc tccctccgga
     1021 cgtagagacc cgcgcggtcc gtctgcagga agtagaggag cagggcctcg gcggtggcgc
     1081 ggggggactc gaccccgtgc cgctccaggt agtcggcggc cctgtcgacc acctcggctg
     1141 gtctcatcgg agggccagga tagtccgttc ggcctattcc ccccgctgag aagggccggt
     1201 aaggtcgttc cagacatagc cgacaccccg gaggtcgccg tgctcagtcg ttcgctcgcc
     1261 gtcaccctca ccgccctcgt ctccttcgcc cttgcggttc cgtccgcctc ttcccgcccc
     1321 gcggacgacg tgatcccggg ggcgtacatc gtcgtgctcc acgagggcgc cgacgccgag
     1381 gaggtcgccc gcgggcacgc cgccgcgcac ggcgccacgg tcgaccacgt ctaccggcat
     1441 gcgctccgtg ggtacgcggc ccggatgtcg ggggccgccg cccggcgggt ggcctcggac
     1501 gcccgggtcc gctcggtgac gccggaccgg gtcgtgcggg cttccgcgca gaccctcccg
     1561 accgggatcg atcgcgtgga cgccgaagcc agcagcacgc aggccgggaa cgggtccggg
     1621 agcgtcggcg tggacgtcgc cgtgctcgac agcgggatcg acacgaagca ccgggacctc
     1681 aacgtcgtcg gcgggaagaa ctgctcgacc gggaagtcct acgctgacgg gaacggccac
     1741 ggcacgcacg tcgccggcac gatcgcggcg aaggacgacg cgaacggcgt cgtcggcgtg
     1801 gcccccgggg cacgcctgtg ggcggtgcgc gtgctgaaca actcggggag cgggtcgtgg
     1861 tcatcggtga tctgcggagt cgactgggtc accgcgaaca agggcacgat cgaggtcgcg
     1921 aacatgagcc tcggcgggtc cggcagcgaa cccgccgcga cgggatgctc gacgggcgac
     1981 gcgctccacg atgcgatctg ccgctccgtc gccgccggcg tgacgtacgt ggtggcggcg
     2041 ggcaacgagt cgaaggattc gaagaacgcg gtcccggccg cctacgacga ggtgatcacg
     2101 gtctcggcgc tcgccgactt cgacggcaac cccggcggcc tcgccgcgcc gacctgccgc
     2161 accgacgagg acgacacctt cgggagcttc tcgaacttcg gcgccgacgt ggatctgatc
     2221 gcgcccgggg tctgcatcct gtcgacctgg aagggcggag gctacgacac gatcagcggc
     2281 acctcgatgg cctcgccgca cgtcgccggc ggcgcggcgc tctacaaggc ggcgaacccg
     2341 ggcgcgagcc ccgcccaggt gcagacggcg ctcgtcgccg ccggcggcac cgactggaac
     2401 aacaccgacg accgcgacgc catcaaggag ccgctgctca acgtcagcag cttctagccg
     2461 aggtccgtca gccgcccgcg cggagctgct tggctcgttc ttcggcgagg agggcgtcga
     2521 tgaactcgtc gagttcgcct tgcagcacct gctcgaggcg gtggaccttg aggttgatgc
     2581 ggtggtcggt cacgcggctc tcggggaagt tgtaggtgcg gatcttctcg gcgcggccgc
     2641 cgccgccgac ctgggcgcgg cgcgcctcgg cctgctcctg ctgctgacgc tcctgctcca
     2701 tctggaggag ccgggcgcgg aggtagcgca tcgccttgtc gcggttctgg agctgtgagc
     2761 gttcctcctg cacgctcacc acgatccccg tcggcttgtg cgtcagcctg accgcggagt
     2821 ccgtcgtgtt caccgactgc ccgcccgggc cgctcgagcg gaagacgtcg acctcgatgt
     2881 cctcggggcg gatctcgacg tcgacctcct cggcctccgg caggaccgcc acgctcgcgg
     2941 ccgaggtgtg gatccggccc tgcgactccg tctgcggcac gcgctggacg cggtgcacgc
     3001 ccgactcgtg cttcagccgc gagtaggcgc cgtcgccgcg gatctccagg atcaccttgt
     3061 cgtacccgcc gaggctcgag gggctcgacg cgaggacgtc ggtcttccag cgccggcgct
     3121 cggcgtactt caggtacatc tcgaggaggt cgccggccca gagcgccgct tcctcacccc
     3181 cttcgcccgc gcggatctcc acgacgacgt tcttctcgtc gttggggtcc cgcggcagca
     3241 gcaggacctc gatctgcgcc cggagctcct cggcctgggc ggcagcgcgg tcggcctcct
     3301 gctcgtagaa ggcacggctc tccggatccg actcgtccgc cgcgagggcg cgagcctcct
     3361 cggcgtcggc ggtcgccttc ctccaggcgc gggaagcggc cacgatctcc ccgagctcgc
     3421 ggtggcgctt cccgagatcg cggagcttcg tgggatcaga ggcgacctcg ggcgaggaga
     3481 gctcctggtt cagctcttcg tacgcggcct cgatctcctc gagccggtcg cgcacggcct
     3541 cacctgccgc cgtcgtcggg ctcgaccggt gccgcggcgc cctcgaccgg cggagcggga
     3601 gactcggcct ccgcgccggc acccgcctcc tgctcgcggc ggatcacctc gccgaacgac
     3661 gtgatcgcca cctcgatctg gtcgggggtg ggcggcttcg tcgtgatctt ctggagccac
     3721 aagcccgggg tcatcagcgc gcgcatgaac ctcgactccg agtagcgggc cccgagacga
     3781 agggcctcgt aggcgagccc ggcgatcagc gggatcgcga tgatgcgcga gccgatccgc
     3841 cacgcgagcc cgggcgtgcc gaacagcgcg aacacgaaga tcgtcagcac catcacgatc
     3901 agcaggaagt tcgtcccgca ccgcacgtgc agggtcgagt agcggtcgac gcgctccggg
     3961 tcggaccagt cgtcgtcgtg ctcgtacgcg gcgatcgtct tgtgctccgc gccgtggtac
     4021 tggaacacgc ggcggatgtc ccgcatccgc ccgatcgccc acaggtagcc gaggaacagc
     4081 agcacgcgga agatcccctc ccccacgttc agccagacgc cggaggcgac gcggtccttc
     4141 gcgaggttcg acaggaacgc cgggaggacg atgaacaccc cgatgaacag caccatcgcg
     4201 aacgtcatcg acagggccat ctgacgcggg gtgagcttct cctcttcctc gaccgagtgg
     4261 ttcgccgaga tggtcagggc cttgacgccg atcgcaagcg cctgcccgag cgcgatgacc
     4321 ccgcgcaggc ccggcttcgc gaggatcttc acgcgcttgg cgaccgagtc gatctcgtgc
     4381 gcctcggtgt ggatcccgcc gtcgggcttg cggaccgcga tcgcccagag gtcgcggccc
     4441 cgcatcatca cgccttcgat gacggcctgg cccccgtagt agtgctcctt ctgcggcata
     4501 ggggcattct acggggccgc ggcgggcggc cctcagcagg agggctgggg cagctccgcc
     4561 tcggggtcgt tcggcgtggt ctcgagcgcg atcgcggccg agggctgcgg ctggctctgg
     4621 tcctggagcg acgtgacgat cgtcccgtcc ggcaggaggg caacggtcgc gaagtcgaag
     4681 cggatgctcg ccccgacgcc gctgcccgcg ttcaggttgc cagccccggc cacgtgcgtc
     4741 atcaggtgct gcggcgcgcc gcacccggtc gcgaactcga ggacgtagtc ctggtcggtg
     4801 ttcgtcgagg agcggtgggc gtgcaccccg acgatcgcgc ggccgagggc gccggaggcc
     4861 ttcgcgtccc actcctcgac cgtcgacccg gccggcagcg aggcggtcgt cgtgtcccac
     4921 gtccgtccgc cgtcacgcga gagccggtaa gtcacggtcc cgcccgaggc gttcagcaga
     4981 tgggcgtggc cgttcgagtc gatcagcagg cgccccggcg gcaggacgct cccgtcaccc
     5041 aggcggaagg gcgaccatcg gagcgttccc ggctcgagga tcatccaccc gccgccgaag
     5101 aggctgtcaa gcgtgtccgc gggccgcgcg agggcgccgc ccccgggcag gggcgtgatc
     5161 gaggacgccg tgatcgactg cgtccagtcg gccgcggcct ggcgcgtggt ggggagccaa
     5221 cgggagacgg tctcctgacc gacaccggtc gcgcgcttgt tcgtcgccag cgtgtagtgg
     5281 acgccgtcga agctgtagga ccagacgtcc ttcgacggcc atccaccctt gaggatcgac
     5341 atgtacgggt tcttcatcgg ccgacccggg aggatgcccg ggcgttcgac cacggcgacc
     5401 cactcgcggt cgtagaacgg cgtgtgcagg acgttgttct ggtacgtcca cagcttcgtc
     5461 gcggcatccc acttgaagcc gaggatccgg tcgccggaat agacgtccca cgccaccccg
     5521 aggacgtcgc cgttcgcggc gaccgtgatg gtgccctcgc cccccatgaa cggctcgatc
     5581 ggcgcgacgc ggcgccaggt ggcccccttg tcgtcggtga agtggatgta ggtcccgccg
     5641 aagtcgagga ggcgcccgtc ggcggtcgcc gcgaaatggt tctcgcagca gttgcccgtg
     5701 ttccggacga cgcgccaccg ggtcgtgccg atcgcctgcc ccgtccggtc gaggaccgga
     5761 taggtccgga ccgccgccgg gaaggcctgc gagacgacgt tcgccgggag gccatcgatc
     5821 ctcttgcccg ccaccgctcc cggcgctgcg ggcagcagga ccaggagggg ggccatcgcc
     5881 acgacgatcc gcttcatcgc cggaggattc gacgtccggc agccgacctc ctgcctcgcc
     5941 ctgcgacggg tccggcgggt gacccgccac ggaagcggga aaggaacccg ccgtgagccg
     6001 gaaacgaggg tcccgtcgat ggaccgagac ggtgtccgtg gtgagagagg cgctcgtccg
     6061 cggatacgac gaccgcatct acaacctcgc ggccgagatc gccttctacc tcgtcgtcgc
     6121 gctccccgcg gtcgtggtgg ggg
//
LOCUS       DATLGG010000333         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_125637, whole genome shotgun sequence.
ACCESSION   DATLGG010000333 DATLGG010000000
VERSION     DATLGG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125637"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VM638_09460"
     CDS             complement(<1..225)
                     /locus_tag="VM638_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12684.1"
                     /translation="MELSEPIAVERDFTTFSVRDGRLAPVTCVVCGCRLERRADDTWW
                     HFSGRGDRDARGCIVACAEQPHYGGVAAALG"
     gene            402..1817
                     /locus_tag="VM638_09465"
     CDS             402..1817
                     /locus_tag="VM638_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HVM12685.1"
                     /translation="MAIQQQDPARIASLELAADTLTPVAAALRLGSRCAFLLESVEEG
                     ARYGRYSIVGVHGRTLEVRDDQARLSGPDGRREFTARDPLEALREVLPASVPAVTDPA
                     FSLASGVGYLAYETAARWERVPVPSSDPLELPDAVFHLPSAVIVFDHLAQVARLATLD
                     DDAASERLEEVARALARPVAAEVPARFAAGAVPPEQDPAVRSRYESGVARLVEEIHAG
                     EMLQCVLARRFSQSTPRSPIEIYRSLRRINPSPYLFYLDLDGVADGAALVGASPELLV
                     RVRGDEVVTRPIAGTRPRAEDPAEDARLEEELRADPKELAEHAMLVDLGRNDVGRVAA
                     PASVEVTTLRAVERYRHVMHLVSEVHGRLRAELDAFDAVRAVFPAGTLTGAPKVRAMQ
                     AIAELEGERRGPYGGAVGYFSPHEVECAITIRSAVIRDGVATVHAGAGVVADSVPERE
                     AAEVAAKAASMLAAIGAEAGT"
     gene            1817..2401
                     /locus_tag="VM638_09470"
     CDS             1817..2401
                     /locus_tag="VM638_09470"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HVM12686.1"
                     /translation="MRVFLLDNYDSFTYNLVQLLARTGAEVRVVRNDEITVSEVAADA
                     PDAIVISPGPSRPERAGISVELVRTLGPTVPTLGVCLGHQAIGVAYGADVVRVPPVHG
                     KAWPVHHRHEGSFVDLASPFTAARYHSLAIDRASLPPELEVTAWSDDGLVMGVRHTEH
                     PVEGFQFHPESILTDDGEALMAAFLDRARARTPA"
     gene            2398..>2799
                     /locus_tag="VM638_09475"
     CDS             2398..>2799
                     /locus_tag="VM638_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HVM12687.1"
                     /translation="MSGRPEATAAIRLRFDDGAQTLLGIVAAIAEAGGTLRTLATPRI
                     SGGTAEAELEVAGIDGATLQALLGAVAGVREAEATRALDRVFGKRIIVVGGGAQVAQV
                     ALGAVSEADRHNLRGERISVELVRTLGPTVPT"
BASE COUNT          352 a         1033 c         1021 g          393 t
ORIGIN      
        1 gccgagggca gcggcgacgc ctccgtagtg cggctgctcg gcgcaggcca cgatacatcc
       61 cctcgcatcg cgatctccgc ggcccgagaa gtgccaccat gtgtcgtcgg cgcgccgctc
      121 gaggcggcag ccacagacga cgcaggtgac cggggcgagg cggccgtcgc ggaccgagaa
      181 ggtcgtgaag tctcgctcga cggcgatcgg ctcggacagt tccatggccc caccgtacga
      241 ccggcgacgc gagacgtcag cgacgcaccg gtgccggttg gattgcggcg cggttgcgat
      301 ccttgacgct cccgcggggc gcccctagca tgcccatccc gcggaagagc ctccgtcccc
      361 gttcggatgg aggctttgtc gttcccgaga gagaagaacc gatggcgatc cagcagcagg
      421 atccggcgcg gatcgcgagc ctcgagctcg cggccgacac gctcacgccc gtcgcggcgg
      481 cgctccgcct cgggtcgcgc tgcgcgttcc tcctggagag cgtggaggag ggcgcccggt
      541 acggtcgcta ctcgatcgtc ggcgtgcacg gccgcacgct cgaggtgcgc gacgaccagg
      601 cgcgtctcag cgggccggac gggcggcggg agttcaccgc tcgcgatccg ctcgaggccc
      661 tgcgcgaggt cctgcccgca tcggtcccgg cggtgacgga cccggcgttc tcgctggcga
      721 gcggagtcgg ctaccttgcc tacgagaccg cggcgcgctg ggagcgggtg cccgttccgt
      781 catcggaccc gctcgagctg cctgacgcgg tcttccacct tccctcggcg gtcatcgtgt
      841 tcgaccatct cgcgcaggtg gcgcggctcg ccaccctcga cgacgatgcc gcctccgagc
      901 ggctcgagga ggtcgcccgc gcgctggccc ggccggtcgc ggccgaggtc ccggctcgct
      961 tcgccgccgg cgcggtgccg ccggagcagg acccggccgt ccgctctcgc tacgagtcgg
     1021 gggtggcgcg gctggtggag gagatccacg ccggcgagat gctccagtgc gtgctcgctc
     1081 gtcgcttcag ccagtcgacc ccgcgcagcc cgatcgagat ataccgctcg ctgcgccgga
     1141 tcaacccgtc gccgtacctg ttctacctcg acctcgacgg cgtggccgat ggcgccgcgc
     1201 tggtcggcgc gtcgccggag ctcttggtgc gggtccgggg cgacgaggtc gtcacccggc
     1261 cgatcgccgg cacccggccg cgggcggagg acccggccga ggatgcccgc ctggaggagg
     1321 agctgcgtgc cgatccgaag gagctggccg agcacgcgat gctcgtcgac ctcggtcgca
     1381 acgacgtcgg ccgtgtcgcc gccccggcga gcgtcgaggt caccaccctg cgcgcggtgg
     1441 agcgctaccg gcacgtcatg cacctcgtgt cggaggtcca cggccggctc cgcgcggagc
     1501 tcgatgcctt cgacgccgtg cgcgccgtct tcccggccgg gaccctgacc ggtgcgccca
     1561 aggttcgcgc catgcaggcc atcgccgagc tcgaaggcga gcggcgcggg ccgtatggcg
     1621 gggcggtcgg ctacttcagc ccgcacgagg tcgagtgcgc catcaccatc cgcagcgccg
     1681 tgatccggga cggggtggcg accgtgcacg ccggcgccgg tgttgtcgcc gactcggtcc
     1741 cggagcggga ggcggccgag gtggcggcca aggccgcgtc gatgctggcc gccatcggcg
     1801 cggaggcggg cacctgatgc gcgtcttcct gctcgacaac tacgactcgt tcacctacaa
     1861 cctcgtccag ctgctggccc gcaccggcgc ggaggttcgt gtcgttcgca acgacgagat
     1921 cacggtgtcc gaggtcgcgg cggatgcgcc cgacgcgatc gtcatctcgc ccggcccgtc
     1981 gagacccgag cgggccggga tcagcgtcga gctggtccgg acgctcggcc ccaccgtccc
     2041 gacactcggg gtgtgcctgg gtcaccaggc catcggcgtc gcgtacggcg ccgacgtcgt
     2101 gcgtgtcccg ccggtgcacg gcaaggcctg gccggtccac catcggcacg agggctcctt
     2161 cgtcgacctg gcgtcgccgt tcaccgccgc ccgctaccac tcccttgcca tcgatcgcgc
     2221 gtcgctgcca cccgagctcg aggtgaccgc ctggtccgat gacggcctgg tcatgggggt
     2281 ccgccacacc gagcatccgg tcgagggatt ccagttccac cccgagtcga tcctcaccga
     2341 tgacggcgaa gcgctcatgg ccgccttcct ggaccgagcg cgcgcgcgga ccccggcgtg
     2401 agcgggcgtc ccgaagcgac cgccgcgatt cgtctgcgct tcgacgacgg cgcgcagacg
     2461 ctcctcggca tcgtcgcggc catcgccgag gcgggcggca cgctgcgcac gctggccacg
     2521 cccaggatca gcggcgggac cgccgaggcc gagctcgagg tcgccgggat cgacggcgcg
     2581 accctccagg cgcttctcgg tgccgtcgcc ggcgtgcgcg aggcggaggc cacgcgggcc
     2641 ctggaccgcg tcttcgggaa gcggatcatc gtcgtcggtg gcggcgcgca ggtggcgcag
     2701 gtcgcgctgg gcgcggtgag cgaggccgac cgtcacaacc tccgcggcga gcggatcagc
     2761 gtcgagctgg tccggacgct cggccccacc gtcccgaca
//
LOCUS       DATLGG010000334         6718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_188627, whole genome shotgun sequence.
ACCESSION   DATLGG010000334 DATLGG010000000
VERSION     DATLGG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188627"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VM638_09480"
     CDS             complement(<1..1220)
                     /locus_tag="VM638_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVM12688.1"
                     /translation="MSTLIIDRIDLFTPGATVESPGDQPSEIEFTVDLGDSGDYVWVS
                     SSGAASMALGTDGLNYNAGAESPGDVDVELAGVESFLLFGTAADDSLHADGSGNTGAP
                     ATMMVQMVGDDGKDVFVGGSGPDYFAGESGNDVFEGGPGNDRMRFYATSGVQVDLDAG
                     TATGEGSDVMSEVEEVVGTPGNDVIRGTAGADTLYGQEGDDVLEGRGGSDWLDGGDGA
                     DRASYASAPSYVWVDLAVPGPQDTHGAGSDVLESIEGLVGSAFSDILSGTSGPDHLEG
                     GPFRDTLVPRAGDDTVDGGSGKDLLLYEGAEAVTVNLVAGRATGQGDDRLSSIESVQG
                     TDGDDTFIASAGSNIFEGGAGFDTLSYARAGGGVTVDLAVAGAQATGGSGSDALAGVE
                     KLIGSRFGDRRRRA"
     gene            1219..3654
                     /locus_tag="VM638_09485"
     CDS             1219..3654
                     /locus_tag="VM638_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVM12689.1"
                     /translation="MDPALVPSTSISSTLVTVGVAPEQLSKVFEGARTSMTELPFTLI
                     EEVPVVERLTISVLPTSWHDGEAVAGEETTKLAPMTIVVVTIFASDRPRRFCTLHPSQ
                     TVSAYKFDDMGRYPASRMLSGRASTAILGIVPGRARWLVATTVLWLTIIALPVSAQRS
                     LRPGDITVPEGFSIEVAVSGLAAPTMVAFDDQGRILIAESGYCPDPERCPDGPGAPKV
                     TRIEADGRKAVLADGEDFGEEHPVTAVAFHEGQVYVVHAGTVSVIEGDGLRPIITGLP
                     GHGDHQANQLVFKDGFLYLAIGTVTNSAVVGPDNAVFGWLKKPDLRELHDVPCEDVTI
                     AGEPFTSPNPLIDETDVADPLGGEPEVRTSPYAAFGTTHPAGHVVPGNPKCNGAILRA
                     RPDGTDLEVFAWGLRNPYGLEIGPDGEVYATMHGFDARGSRQVENAWDCFYRIQEGAW
                     YGWPDYACDVPVTDPRFQPADQPQPGFVLADHPTTSPPEPIAKFGPHEATNGFAFSPS
                     SEWGPPTTAYIALFGDFTPATGTVDRPVGTEIVRVDTTSGQISDFIHNRVPGRASQQN
                     AGGLEHPSDVTFGPDGAMYIADWGVAHVSVDGLVLEPNSGVVWRVVASPGAAGTGPIG
                     TSMWIWLLVLAALAAATFVASRGERRTASAAQGAWTGALAAVVMGGFAMGVASPILNL
                     PWYAPPRVFATLVMGRGALQDILNFDLVSFVVGLVVVLVLGALLGALFALLLRATARP
                     RTTLAAVLFGLTVFVALHYFVLPLVQPLIPQRGFPPEWYAASFAVYGAALGTLLGLAS
                     QRRSAAPAATSRD"
     gene            3929..>6718
                     /locus_tag="VM638_09490"
     CDS             3929..>6718
                     /locus_tag="VM638_09490"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HVM12690.1"
                     /translation="MKGLDDTLPLTRLSIPGTHDTGAYNEGGAATLTQSMDLGEQLRS
                     GIRAWDIRLRDQGGDKGLGVFHGPVYQRLDFADNVLYDAYMFLEDHPKETVLIRIKYE
                     PENKDTEPDDFDRSIADDLQWYIDRGRVYVPPNPPPADSATTLGDVRGKIVILENFTS
                     RTLHRKFGLAYGRFDVQDVSSLNGFKGLGAKWRDHIRPHLEKAAADPNDQRFYVNYLS
                     GEDVAKGTYPYFVASGHASIDNASPRLLTGWDRYDDGGGSCSEDAACLSEFPCANPRD
                     DPCQVAYEGTNILARDYLIRLRPKRVGMVMADFPGAGLIDAIIWRNPAAPKADAGGSY
                     EVPEGETLTLAASRSSHPLSDTLSYEWDLDADGDYDEEAAGETVEYRWGNDGHRFVAL
                     RVTDSDGDQGFATAQVTVTAVSPTVTELDLWPAFPNLRVEGTAQMLSGKVVDPGWLDT
                     PKAQVAWGDGSEEEAAGSVENDPPEATLSFETDHTYGDDDEYTIVVCPSDEDGAGPCE
                     ERSLEILNLPPGVEISDSQATTVPEGTPLGVSASATDIGWLDELSATVDWGDGSTGAA
                     TVNVTGEGPPRLQANVTASHPYGDNGAHRVRLTVADDDGGEHADGFDVTVSNVAPAVQ
                     AGRSDVSLTEGPEGGLLEATATFTDPGWLDEHLAFIDWGDGTVDPVAVTRSDGRVGPG
                     GAAGALRGSHTYGDDGTFTGRFIVVDDDGGIGVDPFTVRVENVDPTAEIDRRSEITVN
                     GRAIFLGRGGTPIGFATRVTDPGSDDVEVRWDFGDGSPISVTTSLSDPPTPDADPSTD
                     LHPRDVLMEQEHVYAPCAWTLVVRSVDDDFGSSPQDQVGVVIRGNAERTLNRSAWRRS
                     FETSPEAEAATLECYLNSVGFLSDVFSELRSSDDAAAASEVLRGGGTADAETRSFDGE
                     LLAAWLNFAAGAIDLD"
BASE COUNT         1083 a         2316 c         2233 g         1086 t
ORIGIN      
        1 tctgcgcggc gacgacggtc cccgaacctg gagccgatca gcttctcgac gcccgcgagc
       61 gcatccgatc ccgaaccgcc ggtcgcctgc gcgccggcga ccgcgagatc caccgtcacg
      121 cctccgcccg ctcgggcgta cgagagggtg tcgaacccgg caccgccctc gaagatgttc
      181 gatcctgcgc tcgcgatgaa ggtgtcgtct ccgtccgtcc cctgaacgct ttcgatgctc
      241 gacagacgat cgtcaccctg gcccgtcgcg cgtcccgcca cgaggttgac cgtgacggcc
      301 tccgctcctt cgtagagcag caggtccttc ccgctcccgc catctacggt gtcgtccccg
      361 gcgcgaggca cgagcgtgtc ccggaacggc cccccctcga gatgatccgg cccggacgtc
      421 ccggagagga tgtcgctgaa cgcggacccg acgagtccct cgatgctttc gagcacgtcg
      481 ctcccggctc catgggtatc ctgcgggccc gggacggcga gatccaccca gacgtacgag
      541 ggtgccgagg cgtacgacgc tcggtcagcg ccatcgcccc catccagcca gtcggaccca
      601 ccgcgcccct cgagaacgtc gtcaccctcc tgaccgtaga gggtgtcggc tccagccgtt
      661 ccgcgtatca cgtcgttccc gggtgtcccg accacctctt ccacctcgct catcacgtcg
      721 cttccctcgc cggtcgctgt ccccgcgtcc aggtccacct ggaccccgga cgtcgcgtag
      781 aaccgcatcc ggtcgttccc gggacctccc tcgaacacgt cgttcccgga ctcccccgcg
      841 aagtagtcgg gtcccgaacc tcccacgaag acgtcctttc cgtcatcgcc gaccatctgg
      901 accatcatcg tcgccggggc ccccgtgttg ccggacccgt ccgcgtggag cgagtcatcc
      961 gccgcggttc cgaacaggag gaacgactcc acgcccgcaa gctccacatc cacatcaccg
     1021 ggcgattcgg cgccggcgtt gtagttcaac ccgtcggttc ccagcgccat ggaagccgcc
     1081 cccgaggagg agacccacac gtagtccccg gagtcgccca gatcgacggt gaactcgatc
     1141 tcgctcggct gatcccccgg cgattccacg gttgcccccg gggtgaagag gtcgatcctg
     1201 tcgatgatga gggtggacat ggatcccgcg ctcgtcccgt cgacgtcgat ctcgtcgacg
     1261 ttggtcaccg tgggcgtcgc accggagcag ctgagcaagg tgttcgaagg cgcacgcacc
     1321 tcgatgacgg agctgccatt caccctgatc gaggaggttc cggtcgtgga aaggctcacg
     1381 atcagcgtgt tgccgaccag ctggcacgac ggcgaagccg tcgccggcga ggagacgacc
     1441 aagctggctc ccatgacgat cgtggtggtg acgatcttcg cgagcgatcg gccgcgacgg
     1501 ttctgcaccc ttcatccctc ccagacggtt tcggcgtata agttcgacga catgggtcga
     1561 tatcctgctt ccaggatgct ctccggcaga gcttcaaccg ccatcctggg catcgtcccg
     1621 ggccgggcga ggtggctggt cgcgaccacc gtcctgtggc tgacgatcat cgcccttccc
     1681 gtgtctgcac agcggtcgct gcgccccggg gacatcacgg ttcctgaggg gttctcgatc
     1741 gaggtcgcgg tctccggtct cgccgcgccg acgatggtcg cgttcgatga tcaaggccgg
     1801 atcctgatcg ccgagtccgg gtactgcccc gatcccgagc ggtgcccgga cggcccagga
     1861 gccccgaagg tcacccgcat cgaggcggat ggccgcaagg cggtcctggc ggacggcgag
     1921 gacttcggcg aagagcaccc ggtgacggcg gtggccttcc acgaggggca ggtctacgtc
     1981 gtccacgccg ggaccgtctc ggtgatcgaa ggcgatggcc tgcgtccgat catcacgggt
     2041 ctccccggcc acggggacca ccaggccaac cagctcgtgt tcaaggacgg cttcctgtac
     2101 ctcgccatcg ggaccgttac gaactccgcg gtcgtgggcc cggacaacgc ggtgttcggc
     2161 tggctgaaga agcccgacct gcgcgagctg cacgacgtcc cgtgcgagga cgtgacgatc
     2221 gccggggagc cgttcacctc cccgaacccc ctgatcgacg aaaccgacgt cgccgatcca
     2281 ctcggggggg agcccgaggt caggacgagt ccgtacgcgg cgttcggaac cacccacccc
     2341 gctggccacg tggtgcccgg caacccgaag tgcaacggcg cgatcctccg cgcacggccg
     2401 gacggaacgg acctcgaggt tttcgcatgg gggctccgca acccgtacgg gctcgagatc
     2461 ggccccgacg gtgaggtcta cgcgacgatg cacggcttcg acgcgcgtgg ctcgcgccag
     2521 gtcgagaacg catgggactg cttctaccgg atccaggagg gcgcctggta cggctggccg
     2581 gactacgcgt gcgacgtgcc ggtcaccgac ccgaggttcc agcccgcgga tcagccccag
     2641 cccgggttcg tcctcgctga tcacccgacg acgtcgcccc ccgaacccat cgcgaagttc
     2701 gggccgcacg aggcaacgaa cggcttcgcc ttctccccct cgagcgagtg gggtccgccg
     2761 acgaccgcct acatcgcgct gttcggcgac ttcacgccgg cgacggggac cgtcgatcgt
     2821 cccgtgggca ccgagatcgt ccgcgtcgac accaccagcg ggcagatctc cgacttcatc
     2881 cacaaccggg tgccgggaag ggcctctcag cagaacgcag gtggactgga gcatccgtcg
     2941 gacgtcacct tcggccccga cggagcgatg tacatcgccg attggggagt ggcgcacgtg
     3001 agcgtcgacg gtctggtgct cgagccgaac tccggcgtcg tgtggagggt cgtcgcctct
     3061 ccgggcgccg ctggcaccgg tccgatcggt acgagcatgt ggatctggct gctcgtgctc
     3121 gcggcgctcg cggccgccac cttcgtcgcg tcgcgcgggg agcgccggac cgcctcagcc
     3181 gcgcagggag catggaccgg cgccctggcg gccgtggtca tgggcggttt cgccatgggc
     3241 gtagcgtccc cgatcctcaa cctcccgtgg tacgcgccgc cacgcgtctt cgcgacgctc
     3301 gtgatgggcc gcggcgcgct ccaggacatc ctgaacttcg atctggtctc gttcgtcgta
     3361 gggctcgtgg tcgtgctcgt cctcggggcg ttgctgggcg cgctgttcgc gctgctcctg
     3421 cgggcgaccg cccggccacg gactaccttg gcggccgtcc tcttcggcct cacggtcttc
     3481 gtcgcactcc actacttcgt cctgccgctc gtgcagccct tgatcccaca acgtggcttc
     3541 ccgcccgagt ggtacgcggc gtcgttcgcg gtgtacgggg cggcgctggg gaccctgctg
     3601 ggcctggcga gccagcgaag gagcgccgcg cctgcagcga caagcaggga ttgacagtaa
     3661 ctgctggtcc ggggacacta tccggacaca atcggatgaa gcgggcatct gggagggagc
     3721 tgagctcggg atccctcatc ccggccacct gccgcacggc caggatgtct cgcgctaggg
     3781 agggagcatg acccgatcgc gtcttcgcag gcccgtaacc gtttcgctgg cagcgctcgt
     3841 tacactcggg cccgctggtc cggcctcgtc gcatccgacg gacgcctacg acaccaccgg
     3901 gaaccaggta gcgtctcgag cggagtggat gaagggtctc gatgacacgc tcccattgac
     3961 gcgcctctcg atcccgggca cccatgacac cggggcgtac aacgaagggg gggccgctac
     4021 cctcacgcag tcgatggacc tcggcgagca gctgcgctcc gggatccggg cctgggacat
     4081 ccgactgagg gatcaaggcg gagacaaggg cctcggggtg ttccacggcc ccgtatacca
     4141 gaggctggac ttcgcggata acgtcctgta cgacgcctac atgttcctcg aggatcaccc
     4201 gaaggaaacg gtcctcatta ggatcaagta cgagccggag aataaggaca ccgagccgga
     4261 cgacttcgac cggagcatcg ctgacgacct gcagtggtac atcgaccggg gcagggtgta
     4321 cgtgccgccc aacccgccgc ctgcagactc cgcgacgacc ctgggggacg tgcgcgggaa
     4381 gatcgtcatc ctcgaaaact tcacaagccg taccctgcat cgcaagttcg ggctcgcgta
     4441 tgggcgcttc gacgtgcagg acgtctcgag tctcaacggc ttcaaggggc tgggagcgaa
     4501 gtggagggat cacatcaggc cgcacttgga gaaagcggcc gccgacccga acgaccagag
     4561 gttctacgtg aactacctca gcggcgagga tgtggcgaag ggcacgtacc cgtacttcgt
     4621 cgccagcgga catgcatcca tcgacaatgc ctcgccccga ctgctcacgg gttgggatcg
     4681 ctacgatgac ggcggcggga gttgcagcga agacgctgcg tgcctgtccg aattcccatg
     4741 cgcgaacccg cgcgatgatc cgtgtcaggt ggcgtacgag gggacgaaca tcctcgcgcg
     4801 tgactacctg atccgcttgc ggcctaagcg ggtgggcatg gtgatggcgg acttcccggg
     4861 cgccggtctc atcgacgcca tcatctggcg caaccccgcg gcgcccaagg ccgatgccgg
     4921 tggctcctac gaggtgccgg agggcgagac tttgacgctg gctgcttcca gatcgtccca
     4981 cccgttgtcc gacacgttgt cctacgagtg ggacctcgac gctgacggag actacgacga
     5041 agaagccgca ggcgagaccg tggagtaccg ctggggcaac gacgggcacc ggttcgtggc
     5101 ccttcgcgtg accgactccg acggtgacca gggcttcgcg accgctcagg tcaccgtaac
     5161 cgctgtgtcc ccgaccgtga ccgaactaga tctctggccg gcgttcccga acctgcgcgt
     5221 cgaaggaacg gcccagatgc taagcgggaa ggtcgtcgac ccgggctggt tggacacgcc
     5281 caaggcgcag gtcgcctggg gggacggctc cgaggaggaa gcggccggct cggtggagaa
     5341 cgacccgccc gaggccaccc tctcgttcga gacggaccac acctacgggg acgacgacga
     5401 gtacacgatc gtcgtgtgcc cctcagacga ggatggtgca ggaccatgcg aggagcgctc
     5461 cctggagatc ttgaacctgc ctccgggcgt cgagatctcc gacagccagg ctaccaccgt
     5521 ccctgagggg acccctctgg gcgtctcggc ctcggcgacc gatatcggct ggctcgatga
     5581 gctttcggcg acggtcgact ggggagatgg atcgaccggg gcggcgacag tcaacgtcac
     5641 cggtgagggt ccaccgcgac tgcaggcgaa cgtcacggca tcgcacccgt acggtgacaa
     5701 cggggcacat cgggtccggc tgacggtggc cgacgacgac ggcggcgagc acgccgacgg
     5761 cttcgacgtc acggtctcca acgtggcgcc cgccgtgcag gccggtcggt ctgacgtttc
     5821 gctcaccgag gggccggagg gtgggctgtt ggaggcaacg gccaccttca cggatcccgg
     5881 atggctcgat gagcacctgg cgttcatcga ctggggcgac gggacggtcg atcccgtcgc
     5941 tgtcacgcgg tcggatggcc gagtcgggcc cggcggggcc gcgggcgcgc tcaggggctc
     6001 ccacacgtac ggagacgacg gcacgttcac gggacgtttc atcgtggtgg atgacgatgg
     6061 agggatcggg gtcgatccgt tcaccgtccg cgtcgagaac gtcgatccga ccgcagagat
     6121 cgatcgtcgg tcggagatca ccgtcaatgg ccgggcgatc ttcctcggga gaggaggcac
     6181 tccgatcggc ttcgccaccc gcgtaaccga tccggggtcg gatgatgtcg aagtgcgatg
     6241 ggacttcggc gatggctcgc cgatctcggt cacgacctcg ctgagcgatc cgccgacccc
     6301 cgacgccgac ccatcgacgg acctgcatcc acgcgatgtc ttgatggagc aggagcatgt
     6361 ctacgctccc tgcgcctgga cgctcgtggt gcggtcggtc gatgacgact tcgggtcctc
     6421 gccgcaggat caagtgggcg tcgttatccg cgggaacgcg gagcgcaccc tcaaccgctc
     6481 cgcctggagg cggtcgttcg agacgagccc cgaagcggag gccgctacct tggagtgcta
     6541 cctcaacagc gtcgggttcc tgagcgatgt cttctcagag ctccggagct cggacgacgc
     6601 cgctgccgcc tccgaggtct tgaggggagg aggcaccgcc gacgccgaga cccgttcctt
     6661 cgacggagag ctgctcgccg cgtggctgaa cttcgcggcc ggcgccatcg atctcgac
//
LOCUS       DATLGG010000335         8068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_189166, whole genome shotgun sequence.
ACCESSION   DATLGG010000335 DATLGG010000000
VERSION     DATLGG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189166"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..2334
                     /gene="ligA"
                     /locus_tag="VM638_09495"
     CDS             310..2334
                     /gene="ligA"
                     /locus_tag="VM638_09495"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVM12691.1"
                     /translation="MATKEERRRAEAKRRVEELRDVIHHHDYRYYVLDDPELSDAEYD
                     ELMRELRALEEEFPELITPESPTQRVGGEPTQLFAPAEHRAPMLSLDNVFSWEELNAW
                     AKRVERVIGRQADFVCELKIDGLAVALTYEEGAYVRGATRGNGFVGDDITTNIRTIRA
                     VPVRLRGEEHPKLLEVRGEVYLPVKAFERLNEELLDAGQRPFANPRNAAAGSLRQKDP
                     RVTASRPLRLWCHGVGVADGRRFSRHSEALEHLREAGLPVNPTTERLGSLEEVFAYCE
                     RWEKERHSVDYEIDGIVVKVDQIALQEELGATAKAPRWAIAYKFPPEERTTQLRKIFV
                     HTGRTGVVTPFAMLEPVFVGGVTVTTATLHNEDEVARKDVREGDTVVVRRAGEVIPEV
                     IGPVVTKRKRGARRWRFPKECPSCGTALVRPEGEAAWRCPNKRGCPSQNIEWLFHFAS
                     RGAMDIEHLGYKTGMALMDRGWVKDPADVYALTAERLAELPGFKEKAIANLLAAIEGS
                     KDRPVWRLLVGLNIRHVGSTVAQMLTDAFPSIDDLAAASVEELEAVEGIGPIVARAVH
                     DWFRDRTNRRLLEKLRAAGVRMKDPRRPKKKGGPLDGKTIVLTGGLEGMSRDEAKRAA
                     EEAGAKVTGSVSKKTDFVVVGTDPGSKHDKALQLGVETIDEQQFLKRLGR"
     gene            complement(2350..4122)
                     /locus_tag="VM638_09500"
     CDS             complement(2350..4122)
                     /locus_tag="VM638_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12692.1"
                     /translation="MTLLGGVAPATASGARPHKGSGSTPHEELRRAVSCQQLSGRCVG
                     LAPLLPASEESLDVDRPAAPTTAPDGSGAVCQTPGLPSPVQDDWCERWMTKRGLGPHG
                     VTEVPRAAATSTDGSEVYVLAHAATSDPLGAGILAFDAATGAWRRTIPVPYRFLGPQS
                     EFFSYLLATGDDLLVVSGYVRVRVETPDGFTRQTNFLTVAYSPTGELRWWSRYEGPMS
                     GRGADHGDWPGSLTISPDGAQVIVTGISTRPGDYQPHPWYRDLSVAPDEVATISYDTS
                     TGSERWLERFDTGDLEFTYGMTIGPRGDALYINTMSWEFESTVLAYGDLGHPEGPDLL
                     WSHAEDPSSFLAPVAIASTSDGVVVGGYSLSNPSGTLDYGVVALDAHGKVRWRANHSN
                     VYTILGTDYTSSDIPVAMATDGDRIYVTGYAFEWDAAYAIGDPSPSIIGSTVAFDVAS
                     GALAWQRIYRFPGHRAHFPTSIHTAGGRVYVPAMDSECNFYVRDASYYCKSTTINLVT
                     LDIDASTGAVRRVARFNHARSVALPALQPSLPTAPGFNIFGTSDLHPDTQQMTIAALL
                     DSPCIDTCLPGARYRMGLISYDGS"
     gene            complement(4125..5819)
                     /locus_tag="VM638_09505"
     CDS             complement(4125..5819)
                     /locus_tag="VM638_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVM12693.1"
                     /translation="MKKVLCLLTAAIAISTAMTSLAAASEPTGGGSPVDCLIPGSDDR
                     CERWAHRYTEGEGDAPFGKHNQHRAEMGAISPTGDRAFIVGSVVDGSRICPGNPPTLC
                     MDMQTVAYDAVTGNILWVKQQSPPWENAHSLPRTVVVSADGSTVFTVSTVFVGSGSAR
                     DTLRDLHIVAYDAATGARRWVRTYDGPARMQEDAFAAAVSPIDDRLFVAAASTSTTLP
                     VSTYGYSPLHMTRGTTAAGLEVDVDFALLSFEGASGELMDERRYGGATFRVDGETRAS
                     WDVPVDIEVSPDGQRIVVSGLSDGRQAYATGSPHSTAVAWDASDEEPGTGWDLAWVRE
                     VAGIPLGLGVFPRQAMAVFAGAYGDGGTESNPYAVLGLDLHTGEPRWEKRYALGGLGG
                     QLEHPRGLLADAERGMVALPGISRYPTSSTGTMGVLALRASDGGTAWTKRLRDPMSNA
                     TIIFNYDVDGMPLGAIDARSGHVQVTSTRGGHLATFSLDGATGAQRWAALFEKPLRSG
                     SYFEESGGDWPVAMGAAGGTVVVFGNSYIPRRQTCSFGPAFTFCPRDDFLTVAYEV"
     gene            complement(5863..7365)
                     /locus_tag="VM638_09510"
     CDS             complement(5863..7365)
                     /locus_tag="VM638_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12694.1"
                     /translation="MIKRDLGPQGAAEAPYAITSSKDGSEIYVLASGATLDAYGAAVL
                     VFDAANGAWRRTIPVPYRFLGPNSDFSPYLLAAGDGSVFVSGFLRVREETPEGFARHQ
                     NFLTASYSPTGDLRWWRVYRGPMSGRGTEHGEFPVGMTLSPDGRQLIVTGVSTRPGPY
                     QADPRYIDRYVPPDDVATVAYDPATGTERWVERFDSGKLEFPYGFAVGPDGDDLYVNV
                     MSWRFESTLVAFRGLRDTEDGPELSWTHSEDPASFLAPIAIAATRDTVVLAGVSLSNP
                     SNTVDYGIVAVGLDGKVRWRASHSNVYTIAGTDYTSTDLPMAMVADDDRVYVTGYAYE
                     WDALYALGDTSPAMVPSTVAFDLATGSLSWQKTYRFPGHRIHFPFGLHLAGSRLYVPG
                     LDSECNWYVMNPNYYCKSTMINLVTLDHDAATGSLQRVARFNHARSVSVPGLQPSPPA
                     APGFALYYGSTLGPDRQLSIVTLFDAPCVECHPAARWRLGLVAYGPPQGS"
     gene            complement(7736..>8068)
                     /locus_tag="VM638_09515"
     CDS             complement(7736..>8068)
                     /locus_tag="VM638_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVM12695.1"
                     /translation="DPRSEATIIFNYDSDVRPMAALDPASGHVQLTMLREGRLAALSL
                     DHATGAQRWASVFDHPVGGTELPERASNFPFEIEAAGGTAVIFGESYMTSPLQAARSD
                     LLTIAYEV"
BASE COUNT         1291 a         2662 c         2896 g         1219 t
ORIGIN      
        1 ccctcgaggt cgccggcgat ctgcacctcg aggatccccg ctgcggggtc ctcgacgcgc
       61 cgctgcaggg tcagccgcaa cgtgtagggc agcgcggagc ggatgtgcag gcgtgcggcg
      121 tcgtcgtcgg ttcgctccac cgaacgaacc tgccgccacc acctcgggta tccggcgagg
      181 tcacggagga tctcgaagac ctcgccggtg ggcgcttgca ctcgccacgt ggaacggaag
      241 cggtagcggg tgcctcgcac ggcccgatcg tagcgggagg cgtcggcgac ggtcggtatc
      301 atcgccccga tggcgacgaa ggaggaacgg cggcgggccg aggcgaagcg ccgcgtcgag
      361 gagctccgcg acgtcatcca tcaccatgac taccggtatt acgtgctcga cgaccccgag
      421 ctctcggacg ccgagtacga cgagctcatg cgtgagctcc gtgcgctcga ggaggagttc
      481 cccgagctga tcacgccgga gagcccgacc cagcgggtcg gcggagagcc gacgcagctc
      541 ttcgcacccg ccgagcaccg tgcgccgatg ctctcgctcg acaacgtttt ctcctgggag
      601 gagctgaacg cgtgggcgaa gcgggtcgag cgcgtgatcg ggcgccaggc ggacttcgtg
      661 tgcgagctga agatcgacgg cctcgcggtg gcgctcacgt acgaggaggg cgcctacgtg
      721 cgcggggcga cgcgggggaa cgggttcgtc ggagacgaca tcaccacgaa catccgcacg
      781 atccgcgccg ttcccgtgcg cctgcgcggc gaggaacatc cgaagctcct cgaggtccgg
      841 ggtgaggtct acctaccggt gaaggccttc gagcggctga acgaggagct cctcgatgcc
      901 ggccagcggc ccttcgcgaa tccgaggaac gccgccgccg gcagcctccg gcagaaggat
      961 ccgcgcgtga cggcctcgcg gccgttgcgc ctctggtgcc acggcgtcgg ggtcgccgac
     1021 ggtcgccgct tctcgcgcca ctccgaggcg ctcgagcatc tccgcgaggc gggcctcccg
     1081 gtcaacccca cgaccgagcg cctcgggtcg ctcgaagagg tgttcgccta ctgcgagcgg
     1141 tgggagaagg agcgccactc ggtcgactac gagatcgacg ggatcgtcgt gaaggtcgat
     1201 cagatcgcgc tccaggaaga gctcggggcg accgcgaagg ccccgcgttg ggcgatcgcc
     1261 tacaagttcc ccccggagga gcggacgacg cagctgcgga agatcttcgt ccacacgggc
     1321 cggaccgggg tggtcacgcc gttcgcgatg ctcgagccgg tgttcgtcgg cggcgtcacg
     1381 gttacgaccg cgacgctgca caacgaggac gaggttgcgc gcaaggacgt gcgcgagggg
     1441 gacacggtcg tcgtccgtcg cgccggcgag gtgatccccg aggtgatcgg gccggtcgtc
     1501 acgaagcgta agcggggagc gcggcgctgg cggttcccga aggagtgccc gtcgtgcggg
     1561 accgctctcg tgcggcccga gggggaggcc gcgtggcggt gcccgaacaa gcgtggctgc
     1621 ccctcgcaga acatcgagtg gctgttccac ttcgcctcgc gcggcgcgat ggacatcgag
     1681 cacctcggct acaagaccgg gatggcgctg atggaccgag gctgggtgaa ggacccggcc
     1741 gacgtctacg cgctcacggc cgagcggctc gcggagctgc cgggcttcaa ggagaaagcg
     1801 atcgcgaacc tgctggccgc gatcgagggg tcgaaggacc ggccggtgtg gcggctgctc
     1861 gtggggctga acatccgcca cgtcggctcg acggtggccc agatgctcac cgacgcgttc
     1921 ccctcgatcg acgacctggc ggcggcttcg gtggaagagt tggaggcggt cgagggtatc
     1981 ggcccgatcg tcgcgcgagc cgtccacgac tggttccgcg accgaacgaa ccggaggctg
     2041 ctcgagaagc tccgcgcggc cggtgtccgg atgaaggacc cgcggcgacc gaagaagaag
     2101 ggtgggccgc tcgacggcaa gacgatcgtc ctcaccggcg gcctcgaggg tatgagccgc
     2161 gacgaggcga agcgcgccgc cgaagaggcc ggcgccaagg tgaccggcag cgtttccaag
     2221 aagaccgact tcgtcgtcgt cggcaccgac cccggctcga agcacgacaa ggccctccag
     2281 ctaggcgtcg agacgatcga cgagcagcag ttcctgaagc gcctcggccg gtgatggcgc
     2341 ggcgggaggt cagctcccgt cgtaggagat gaggcccatc cggtaacggg ctcccgggag
     2401 gcacgtgtcg atgcagggcg aatcgagcaa ggccgcgatc gtcatctgct gcgtatccgg
     2461 gtggaggtcc gaggttccga agatattgaa gccgggagct gtcggtaggc tcggctggag
     2521 cgcgggcaga gcgacgctcc gcgcgtggtt gaaccgcgcg acgcgacgga cggctcccgt
     2581 cgaagcgtcg atgtcgagcg tcaccaggtt gatcgtcgtc gacttgcagt agtagctcgc
     2641 gtctcggacg tagaagttgc actcggagtc catcgcggga acgtacacgc gccctcctgc
     2701 ggtgtggatg ctcgtcggga agtgggcgcg gtgcccaggg aaccgataga tcctctgcca
     2761 tgcgagggcg ccgctcgcga cgtcgaacgc gacggtcgag ccgatgatcg agggggaggg
     2821 gtcgccgatg gcgtacgccg cgtcccattc gaaggcgtag ccggtgacgt agatgcgatc
     2881 gccgtcggtg gccatcgcca cggggatgtc ggacgaggtg tagtccgtcc cgaggatcgt
     2941 gtatacgttc gagtggttcg cccgccatcg gaccttcccg tgcgcgtcca gggcgacgac
     3001 gccgtaatca agggttccgc tggggttgga gagggagtat ccccccacga cgacgccgtc
     3061 cgaggtgctc gcgatcgcga cgggggcgag gaacgagctc ggatcctcgg cgtgcgacca
     3121 caggagatcc ggaccctcgg ggtgaccgag gtcgccgtag gccagcacgg ttgactcgaa
     3181 ctcccacgac atcgtgttga tgtacagggc atccccgcgg ggaccgatgg tcatcccata
     3241 ggtgaactcg aggtcgccgg tgtcgaaccg ctcgagccat cgctcgctcc cggtggatgt
     3301 gtcgtaggag atcgtggcca cctcgtccgg ggcgaccgac aggtccctgt accaagggtg
     3361 gggctggtag tcaccgggtc gcgtgctgat gcccgtcacg atcacctgcg ccccatcggg
     3421 cgagatcgtg aggctcccgg gccaatcccc gtggtccgcc ccgcggccgc tcatcggtcc
     3481 ttcgtagcgg ctccaccagc gaagctcgcc ggtaggggag taggccacgg tcaggaagtt
     3541 cgtctggcgc gtgaagccgt ccggtgtctc gacgcgcacc ctgacgtagc cggagacgac
     3601 gagcaggtcg tcgcctgtgg ccaggaggta ggagaagaac tcgctctggg gtccgaggaa
     3661 ccggtacgga acggggatcg tccggcgcca tgctccggtc gcggcgtcga aggccaagat
     3721 gcccgcgccg agtgggtcgg aggtggcggc gtgtgccagc acgtacacct cgctgccatc
     3781 ggtcgacgtt gccgcggctc gcgggacctc ggtcacgccg tgcggaccga gcccgcgctt
     3841 cgtcatccac cgctcgcacc agtcgtcctg cacgggcgag ggcaggcccg gcgtctgaca
     3901 cacggcaccc gatccgtccg gtgccgtcgt gggagctgcc ggacgatcca cgtccagact
     3961 ttcctcggac gcgggaagga ggggcgccag cccgacgcag cgtcccgaga gctgctggca
     4021 cgagaccgcg cgtcgcagct cctcgtgagg cgtgcttcca ctgcccttgt gcggccgcgc
     4081 tcccgaggcc gtcgctggcg cgacgccgcc gaggagcgtc acgctcagac ctcgtaggcg
     4141 acggtcagga agtcgtcacg tgggcagaac gtgaacgcag gaccgaagga acacgtctgt
     4201 cgccgaggga tgtacgagtt cccgaagacg accacggtgc ctccagcagc acccatcgcg
     4261 accggccagt cgccacccga ctcttcgaag tacgaacccg accgcagggg cttctcgaag
     4321 agtgctgccc accgctgcgc gcccgtggcg ccgtcgagtg agaacgtggc gaggtggccg
     4381 ccgcgtgtgg aggtgacctg gacgtgcccg gaccgtgcgt cgatcgcgcc gaggggcatc
     4441 ccgtcgacgt cgtagttgaa gatgatcgtc gcgttgctca tcgggtcccg caggcgcttc
     4501 gtccaggcgg tcccaccatc cgaggctcgc agcgccagca cgcccatcgt tccggtgctg
     4561 gaggtcgggt agcggctgat accgggaagg gccaccatcc cgcgctcggc gtcggccagg
     4621 agccctcgcg gatgctcgag ctgtccgccg aggcctccga gcgcgtaccg cttctcccac
     4681 cggggctcgc ccgtgtggag atcgagcccg agaaccgcgt acgggttcga ctcggtgccg
     4741 ccgtcaccgt acgcgccggc gaagacggcc atcgcctgac gggggaagac gcccagtccc
     4801 agtgggatcc cggccacctc gcgcacccag gcgagatccc agcccgtccc cggctcctca
     4861 tcgctcgcgt cccaggcaac cgcggtcgaa tgcgggctgc cggtcgcgta cgcctggcgg
     4921 ccatcgctga ggccgctgac gacgatgcgt tgcccgtctg ggctgacctc gatgtcgacc
     4981 gggacgtccc aggacgcgcg ggtctcacca tcgaccctga aggtcgcgcc gccgtagcgg
     5041 cgctcatcca tcagctctcc gctggctcct tcgaaggaga gcagggcgaa gtcgacatcg
     5101 acctcgagtc ccgccgcagt cgtcccgcgt gtcatgtgga ggggggagta cccgtacgtc
     5161 gagacgggca gcgtggtcga ggtggacgcg gccgcgacga agagtcggtc atcgatcggg
     5221 gagaccgcgg ctgcgaaggc gtcctcctgc atccgcgccg ggccgtcgta ggtcctgacc
     5281 cagcggcgcg ctccggtcgc ggcgtcgtac gccacgatgt ggaggtcgcg cagcgtgtcc
     5341 ctcgcgctcc cgctcccgac gaagaccgtg gagacggtga acacggtgct cccgtccgcg
     5401 ctgaccacga ccgtgcgagg cagcgagtgc gcgttctccc agggcgggga ctgctgcttg
     5461 acccagagga tgtttcccgt gacagcgtcg taggcgaccg tctgcatgtc catgcacagg
     5521 gtcggggggt tcccggggca gatcctcgac ccgtccacga ccgatcccac gatgaacgcg
     5581 cggtcgccag tgggcgagat ggcgcccatc tcagcgcggt gctggttgtg cttcccgaac
     5641 ggggcatctc cttcaccctc ggtgtaccgg tgcgcccacc gctcgcaccg gtcgtcggac
     5701 ccagggatca gacagtcgac cggtgagcct ccgccggtcg gttcggacgc ggccgcgagc
     5761 gaggtcatcg ccgtcgagat ggcgatggca gcggtcagca ggcagagaac cttcttcatc
     5821 tcccgttcct tccttctcgt gttgtcgatc gtgtctccca catcacgatc cctgcggcgg
     5881 accgtaggcg acgagtccca ggcgccatcg agccgctggg tggcattcga cgcatggggc
     5941 atcgaacagg gtgacgatcg atagctgcct atccggaccg agcgtcgatc cgtaatagag
     6001 cgcgaacccg ggggcggccg gcgggctcgg ttgcaggccg ggaaccgaga cgctccgagc
     6061 gtggttgaag cgggccacac gttgcaggga gccggtcgct gcatcgtggt cgagcgtcac
     6121 gaggttgatc atcgttgact tgcagtagta gttcggattc atcacgtacc agttgcactc
     6181 ggagtcgagc cccggaacgt acaggcggct cccggcgaga tggagcccga aggggaagtg
     6241 gatcctgtgc ccggggaacc gataggtctt ctgccagctg aggctgccgg tcgccaggtc
     6301 gaaggcgacc gtcgagggca ccatcgcggg cgaggtatcc ccgagcgcgt acaacgcgtc
     6361 ccactcgtag gcgtagccgg tgacgtagac gcggtcgtcg tcggcgacca tcgccatggg
     6421 gagatcggtc gacgtgtagt cggtgcccgc gatcgtgtac acgttcgaat ggctggcccg
     6481 ccagcggacc ttcccatcga gcccgacagc cacgatcccg taatccacgg tgttgctcgg
     6541 gttcgacagc gacacgcccg cgaggacgac cgtatcccgc gtggcggcga tggcgatcgg
     6601 agccaagaaa gaggccggat cctcggaatg ggtccacgag agctccggac catcctcggt
     6661 gtcgcgcagc cctcggaacg cgacgagggt cgactcgaac cgccacgaca tgacgttgac
     6721 gtacagatcg tccccgtcgg gacccacggc gaagccatag gggaattcga gcttcccgct
     6781 gtcgaaccgc tcgacccacc gctccgtgcc ggtcgccgga tcgtaggcga ccgtcgcgac
     6841 gtcgtccggc gggacgtatc ggtcgatgta ccgcgggtcg gcctggtatg ggcctggtcg
     6901 cgtgctgacg ccggtcacga tcagctgccg cccgtccggg gagagcgtca tccctacggg
     6961 gaattcgccg tgttccgttc cccgcccgct catcggcccc cggtaaacgc gccaccagcg
     7021 aaggtcgccc gtgggggagt aggacgcggt caggaagttc tgatgccgcg cgaacccctc
     7081 gggcgtttcc tcccggaccc ggaggaagcc ggacacgaag accgatccgt cgccggccgc
     7141 gaggaggtac ggggagaagt cgctgttggg tccgaggaag cggtagggaa ccgggatcgt
     7201 gcggcgccag gctccattcg ctgcatcgaa gacgaggacg gcagcgccgt aggcgtccag
     7261 cgtcgcgccg gacgcgagca catagatctc tgagccatcc ttggaggacg tgatcgcgta
     7321 aggagcctct gcggcgccct ggggtcccag gtcgcgtttg atcacccacc gctcgcacca
     7381 gtcgtcctgg atcggcgagg gggtgcccgg gccggcacat gcaggacccg cgggcgtcgg
     7441 cggtggggtt ggggtagcag ggtccgatcc gggcttgttc gaacgggtct ggggcaggag
     7501 cggacgtatc ccgacgcagc ggccggcgat ctcctcgcac gataggacct gctggagctc
     7561 ctcacgaggc gactcgccca ccgcctgggg cccaggggtc cctgaggccc gcgcaggcgc
     7621 cgtcgccgcg agcagggcga ggaccgccgc ggccgcagcg agcgcacggc ttccccgacg
     7681 ggcacggcga aacgtggaga tcgatctgaa cctcatcgtt ggccccctct catcatcaga
     7741 cctcgtaggc gatcgtcagg aggtccgacc gcgccgcctg caacggggac gtcatgtacg
     7801 actctccgaa gatcaccgcg gtccctccgg cagcctcgat ctcgaagggg aagttcgatg
     7861 cccgttcggg tagctcggtt cctccgacgg gatggtcgaa gacgctcgcc caccgctgag
     7921 caccagtggc gtggtcgagg gagagcgccg cgagccgtcc ttcgcgcagc atcgtcagct
     7981 ggacgtgtcc cgacgcgggg tccagcgccg ccatcggccg cacatcgctg tcgtagttga
     8041 agatgatcgt cgcctcgctc cgcgggtc
//
LOCUS       DATLGG010000336         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13608
            k141_189531, whole genome shotgun sequence.
ACCESSION   DATLGG010000336 DATLGG010000000
VERSION     DATLGG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939490
            Sequence Read Archive: SRR8438990
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 07:59:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,905
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,855
            CDSs (with protein)               :: 1,855
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189531"
                     /isolate="SMAG_U13608"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VM638_09520"
     CDS             complement(<1..2053)
                     /locus_tag="VM638_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVM12696.1"
                     /translation="MLAVLAGAAVMFALALLPTVGSAGRAFAQVSNSFQALGGTEDIR
                     FPRFPERSTVYDVNGRVMATLFFDENRKHVKLDRINQVAREAVLAIEDTKFYEHDGVD
                     MVAVARAALKNLLAGEIEQGASTITQQLARNAFPSVGTERTITRKVAEAQMALRIEQE
                     YTKDEILELYLNRVYFGRGVYGIGTAAEYYFGTKAADLTLPQAATLAGLIASPEKYSP
                     ENDLDAATERRDQVLRRMARVGFITQEEADAAIAEPIELDITPPRKPKSAFFIDFIKR
                     RFLEDERFGATREERKQILFQGGLKIHTTFDPKLERHGLKVIRGHLPLKEDPEAAIAS
                     IDGRTGAIRALVSSQRFGRSQVNLATGEGGTGRQVGSSFKVVTLLAALEQKIPQMKVY
                     NGQHGQPVDCTPWGPPNYRANNADGSGGYMHLWEATRRSVNAVFVQLAVDAGVDNMID
                     VAKRAGIRSELPEVCSLTLGTGEVNALEMANVFQTISNQGKRCRPYAIARILKPNGKE
                     FFKQGRDNCKQVIDKKVAITVANMLKPVVESGTGTAAKLGQWPVFGKTGSTNDLRDAT
                     FGGCTVQICSFVWVGHQKALIPMQNVHGQRVFGGTFPARIWHDFMAEVMKGQKPLDWP
                     PAPKPEAEKVPDVVGMPQEKAERVLAKAGFTAVVKQVASNQPVGIVDRQNPPGGSSVT
                     AG"
     gene            2229..>3186
                     /locus_tag="VM638_09525"
     CDS             2229..>3186
                     /locus_tag="VM638_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HVM12697.1"
                     /translation="MKRTLFGLVALGLALGTAASGSAQEDAHVVEVVELSGVIDPTTA
                     GYLTERIRSAADDGSEVVIVQLDTPGGLVVSMREMVREILRARVPVVVWVGPPGARAA
                     SAGVFLVYASHVAAMAPGTNLGAAHPVDLGGELSGAAEEKAVNDAAKFLRSLAEQRGR
                     DPAFAEETVRRSTSLTSTEAQQRGVIEVVAASVPDLLGQLDGRRVETDAGSRVLVTEA
                     SGEQRVIVRFHKPGLMRRILHAVTDPSIAYLLLVLGFWAIVFEISQPGIGIAGIAGVV
                     ALILAFYALAVLPVNLAALLLVLLGLALLTIDVFTAGLGVFTI"
BASE COUNT          415 a         1123 c         1106 g          542 t
ORIGIN      
        1 tcccggcggt cacgctcgac cctcccggcg ggttctgccg gtcgacgatc ccgacgggct
       61 ggttcgacgc cacctgcttc acgacggcgg tgaagccggc cttcgccagc acccgctccg
      121 ccttctcctg tggcatcccg acgacgtcgg gcaccttctc ggcctccggc ttcggtgccg
      181 gtggccagtc gagcggcttc tgacccttca tcacctcggc catgaagtcg tgccagatcc
      241 gggcggggaa ggtcccgccg aagacgcgct ggccgtgcac gttctgcatc gggatgagcg
      301 ccttctggtg gcccacccag acgaaggagc agatctggac cgtgcagccc ccgaacgtcg
      361 cgtcccgcag gtcgttcgtc gaacccgtct tgccgaacac gggccactgc ccgagcttgg
      421 ccgcggtgcc ggtcccgctc tccacgaccg gcttcagcat gttcgcgacg gtgatcgcga
      481 ccttcttgtc gatcacctgc ttgcagttgt cccgcccctg cttgaagaac tccttgccgt
      541 tcggcttgag gatgcgcgcg atcgcgtagg gacggcagcg cttgccctgg ttcgagatcg
      601 tctggaagac gttcgccatc tcgagcgcgt tgacctcgcc ggtcccgagc gtgagcgagc
      661 agacctcggg gagctccgag cggatcccgg cgcgcttcgc gacgtcgatc atgttgtcga
      721 ccccggcgtc gacggcgagc tgcacgaaga cggcgttcac cgacctcctc gtcgcctccc
      781 acaggtgcat gtaccccccg ctcccgtcgg cgttgttcgc gcggtagttc gggggtcccc
      841 agggggtgca gtcgaccggc tgcccgtgct ggccgttgta gaccttcatc tgcgggatct
      901 tctgttcgag cgccgcgagc agcgtcacga ccttgaagga cgaaccgacc tgacggccgg
      961 tgccaccctc acccgtcgcg aggttgacct gcgagcggcc gaagcgctgg ctgctgacga
     1021 gcgcgcggat cgccccggtc cgcccatcga tcgacgcgat cgcggcctct ggatcctcct
     1081 tgagcggcag gtgcccgcgg atcaccttca gcccgtgacg ctcgagcttc gggtcgaagg
     1141 tcgtgtggat cttcagcccg ccctggaaca ggatctgctt ccgctcctcg cgcgtggcgc
     1201 cgaaccgctc gtcctcgagg aagcggcgct tgatgaagtc gatgaagaag gcggacttcg
     1261 gcttccgcgg cggggtgatg tcgagctcga tcggctcggc gatcgccgcg tcggcctcct
     1321 cctgggtgat gaagccgacg cgtgccatcc ggcgcaggac ttgatcccgc cgttcggtgg
     1381 ccgcgtccag gtcgttctcg ggcgagtact tctcgggcga cgcgatcaac ccggcgagcg
     1441 tggcggcctg cgggagcgtg agatcggcgg ccttcgtccc gaagtagtac tcggccgccg
     1501 tgccgatgcc gtagacgccg cggccgaagt agacccggtt caggtagagc tcgaggatct
     1561 cgtccttcgt gtactcctgc tcgatccgca gggccatctg cgcctccgcc accttccggg
     1621 tgatggtccg ctcggtcccg accgagggga aggcgttccg cgccagctgc tgcgtgatcg
     1681 tcgaggcgcc ctgctcgatc tcgccggcga gcaggttctt cagcgcggcc cgggccaccg
     1741 cgaccatgtc gacgccgtcg tgctcgtaga acttcgtgtc ctcgatcgcc aggaccgcct
     1801 ctcgcgccac ctggttgatc ctgtcgagct tgacgtgctt gcggttctcg tcgaagaaca
     1861 gcgtcgccat cacgcggccg ttcacgtcgt agaccgtcga gcgctcgggg aagcgcggga
     1921 accggatgtc ctcggtgcca ccgagcgcct ggaagctgtt cgagacctgc gcgaacgccc
     1981 gtcccgcgct gccgaccgtg gggagcagcg cgagggcgaa catcacggcg gcgccggcga
     2041 gaacggcgag caagaggacg gggcgcaggt aggcggggac gccttcgcgc cgcgcggcag
     2101 cacgacgagg cgcgcggcgt gtaccggcgc gacgtgcggc agaggctctg gggatcgtgg
     2161 ccatggtggt tgaggggccg gggcaccctc aggatactcg gtccgggagg ccgcgatagg
     2221 attcgcgggt gaagcggacc ctcttcggcc tcgtggcgct ggggctcgcc ctcggcaccg
     2281 ccgcatccgg gtccgcccag gaggatgccc acgtcgtcga ggtcgtcgag ctctcgggcg
     2341 tgatcgatcc cacgacggcc ggctacctca ccgagcggat ccgctccgca gccgacgacg
     2401 gctccgaggt cgtgatcgtg cagctcgaca cgccgggcgg cctcgtcgtg tcgatgcggg
     2461 agatggtgcg cgagatcctg cgcgcccggg tccccgtcgt cgtctgggtc ggtccgcccg
     2521 gcgcccgcgc cgcatcggcc ggggtcttcc tcgtctacgc ctcccacgtc gccgcgatgg
     2581 cgcccggaac gaacctcggc gcggcacacc ccgtcgatct cggcggcgag ctctcgggcg
     2641 ccgccgagga gaaggccgtg aacgacgcgg cgaagttcct gcggagcctg gcggagcagc
     2701 gggggaggga ccccgccttc gccgaggaga cggtccgtcg cagcacgtcg ctcacctcca
     2761 ccgaggcgca gcagcgcggg gtcatcgagg tggtcgcggc gagcgtcccc gacctgctgg
     2821 gccagctcga cggacgccgg gtcgagacgg acgcggggtc gcgcgtgctc gtgaccgagg
     2881 cctcgggcga gcagcgcgtg atcgtgcggt tccacaagcc cgggctcatg cggcggatcc
     2941 tgcacgcggt cacggacccg tcgatcgcct acctgctgct ggtgctcggg ttctgggcga
     3001 tcgtgttcga gatcagccag cccggcatcg gcatcgcggg gatcgccggc gtcgtcgcgc
     3061 tgatcctcgc cttctacgcg ctcgccgtcc ttcccgtgaa cctcgccgcg ctcctcctgg
     3121 tcttgctcgg gctcgcgctc ctgacgatcg acgtcttcac ggccgggctc ggcgtcttca
     3181 cgatcg
//
